Neuropeptide Y prohormone-8
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny AppSub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30020594 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 16Note: Hover over icons to view figure legend
Homology
BLAST of Neuropeptide Y prohormone-8 vs. UniProt/SwissProt
Match: sp|Q27441|NPY_APLCA (Pro-neuropeptide Y OS=Aplysia californica OX=6500 GN=NPY PE=1 SV=1) HSP 1 Score: 45.4394 bits (106), Expect = 1.547e-6 Identity = 27/74 (36.49%), Postives = 36/74 (48.65%), Query Frame = 1 Query: 7 INKCYFLVFILCFMSFIHLNTCNQK------RPM-FDSADAFRNYLRKLNNEYMIAGRPRFGKRRSDFEKELFY 207 + + +V +L M+ + + N + RP F SA R YL LN Y I GRPRFGKR F K F+ Sbjct: 1 MQRVILVVLLLSCMAVLSVRADNSEMLAPPPRPEEFTSAQQLRQYLAALNEYYSIMGRPRFGKRGDSFRKREFF 74
BLAST of Neuropeptide Y prohormone-8 vs. TrEMBL
Match: E3CTI5 (Neuropeptide Y prohormone-8 OS=Schmidtea mediterranea OX=79327 PE=2 SV=1) HSP 1 Score: 154.451 bits (389), Expect = 3.246e-47 Identity = 75/75 (100.00%), Postives = 75/75 (100.00%), Query Frame = 1 Query: 1 MIINKCYFLVFILCFMSFIHLNTCNQKRPMFDSADAFRNYLRKLNNEYMIAGRPRFGKRRSDFEKELFYNLKSNL 225 MIINKCYFLVFILCFMSFIHLNTCNQKRPMFDSADAFRNYLRKLNNEYMIAGRPRFGKRRSDFEKELFYNLKSNL Sbjct: 1 MIINKCYFLVFILCFMSFIHLNTCNQKRPMFDSADAFRNYLRKLNNEYMIAGRPRFGKRRSDFEKELFYNLKSNL 75
BLAST of Neuropeptide Y prohormone-8 vs. Planmine SMEST
Match: SMESG000066803.1 (SMESG000066803.1) HSP 1 Score: 154.451 bits (389), Expect = 9.028e-51 Identity = 75/75 (100.00%), Postives = 75/75 (100.00%), Query Frame = 1 Query: 1 MIINKCYFLVFILCFMSFIHLNTCNQKRPMFDSADAFRNYLRKLNNEYMIAGRPRFGKRRSDFEKELFYNLKSNL 225 MIINKCYFLVFILCFMSFIHLNTCNQKRPMFDSADAFRNYLRKLNNEYMIAGRPRFGKRRSDFEKELFYNLKSNL Sbjct: 1 MIINKCYFLVFILCFMSFIHLNTCNQKRPMFDSADAFRNYLRKLNNEYMIAGRPRFGKRRSDFEKELFYNLKSNL 75
BLAST of Neuropeptide Y prohormone-8 vs. Planmine SMEST
Match: SMESG000069090.1 (SMESG000069090.1) HSP 1 Score: 43.8986 bits (102), Expect = 3.520e-7 Identity = 22/54 (40.74%), Postives = 30/54 (55.56%), Query Frame = 1 Query: 16 CYFLVFILCFMSFIHLNTCNQKRPMFDSADAFRNYLRKLNNEYMIAGRPRFGKR 177 C LV ++C + + K FD + RNY++KLN + I GRPRFGKR Sbjct: 9 CLTLVNVICSQKSLFIEP-PAKPEFFDDPELLRNYIKKLNEYFAIVGRPRFGKR 61
BLAST of Neuropeptide Y prohormone-8 vs. Planmine SMEST
Match: SMESG000058298.1 (SMESG000058298.1) HSP 1 Score: 40.4318 bits (93), Expect = 6.702e-6 Identity = 24/62 (38.71%), Postives = 36/62 (58.06%), Query Frame = 1 Query: 22 FLVFIL----CFMSFIHLNTCNQ------KRPMFDSADAFRNYLRKLNNEYMIAGRPRFGKR 177 F++FIL C +S ++ NQ + +F++A YL+KL+ Y IAGRPR+GKR Sbjct: 10 FVIFILFQNICTLSDYETSSFNQWYDKRDSKLLFNNAKQLLWYLQKLDKMYAIAGRPRYGKR 71
BLAST of Neuropeptide Y prohormone-8 vs. Planmine SMEST
Match: SMESG000050248.1 (SMESG000050248.1) HSP 1 Score: 40.4318 bits (93), Expect = 8.230e-6 Identity = 18/34 (52.94%), Postives = 22/34 (64.71%), Query Frame = 1 Query: 97 SADAFRNYLRKLNNEYMIAGRPRFGKRRSDFEKE 198 S +N L LN EY+IAGRPRFGKR F++ Sbjct: 27 SLAELKNLLSDLNEEYLIAGRPRFGKRNMIFKRS 60 The following BLAST results are available for this feature:
BLAST of Neuropeptide Y prohormone-8 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of Neuropeptide Y prohormone-8 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of Neuropeptide Y prohormone-8 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of Neuropeptide Y prohormone-8 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of Neuropeptide Y prohormone-8 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of Neuropeptide Y prohormone-8 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of Neuropeptide Y prohormone-8 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 1
BLAST of Neuropeptide Y prohormone-8 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 1
BLAST of Neuropeptide Y prohormone-8 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of Neuropeptide Y prohormone-8 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of Neuropeptide Y prohormone-8 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Neuropeptide Y prohormone-8 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of Neuropeptide Y prohormone-8 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of Neuropeptide Y prohormone-8 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 4
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30020594 ID=SMED30020594|Name=Neuropeptide Y prohormone-8|organism=Schmidtea mediterranea sexual|type=transcript|length=280bpback to top Aliases
The feature 'Neuropeptide Y prohormone-8' has the following synonyms
Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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