SMED30020566

Overview
NameSMED30020566
Smed IDSMED30020566
Length (bp)595
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30020566 (SMED30020566) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30020566 (SMED30020566) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top

 

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Homology
[Close]
BLAST of SMED30020566 vs. Ensembl Human
Match: DYNC1H1 (dynein cytoplasmic 1 heavy chain 1 [Source:HGNC Symbol;Acc:HGNC:2961])

HSP 1 Score: 87.4261 bits (215), Expect = 5.501e-20
Identity = 36/72 (50.00%), Postives = 54/72 (75.00%), Query Frame = -3 
Query:   90 LYQSTQTWLQILPKSVTPLQRTIENIKDPMYRCFEREVNSGCNLLASVRKDLEDVAEVCKGAKKPTNHHRSM 305
            L+ +   WL ++P++++ L+RT+ENIKDP++R FEREV  G  LL  VR+DL DV +VC+G KK TN+ R++
Sbjct:  221 LHTTASNWLHLIPQTLSHLKRTVENIKDPLFRFFEREVKMGAKLLQDVRQDLADVVQVCEGKKKQTNYLRTL 292
[Close]
BLAST of SMED30020566 vs. Ensembl Human
Match: DYNC1H1 (dynein cytoplasmic 1 heavy chain 1 [Source:HGNC Symbol;Acc:HGNC:2961])

HSP 1 Score: 87.8113 bits (216), Expect = 1.135e-19
Identity = 36/72 (50.00%), Postives = 54/72 (75.00%), Query Frame = -3 
Query:   90 LYQSTQTWLQILPKSVTPLQRTIENIKDPMYRCFEREVNSGCNLLASVRKDLEDVAEVCKGAKKPTNHHRSM 305
            L+ +   WL ++P++++ L+RT+ENIKDP++R FEREV  G  LL  VR+DL DV +VC+G KK TN+ R++
Sbjct: 1252 LHTTASNWLHLIPQTLSHLKRTVENIKDPLFRFFEREVKMGAKLLQDVRQDLADVVQVCEGKKKQTNYLRTL 1323
[Close]
BLAST of SMED30020566 vs. Ensembl Human
Match: DYNC1H1 (dynein cytoplasmic 1 heavy chain 1 [Source:HGNC Symbol;Acc:HGNC:2961])

HSP 1 Score: 87.8113 bits (216), Expect = 1.164e-19
Identity = 36/72 (50.00%), Postives = 54/72 (75.00%), Query Frame = -3 
Query:   90 LYQSTQTWLQILPKSVTPLQRTIENIKDPMYRCFEREVNSGCNLLASVRKDLEDVAEVCKGAKKPTNHHRSM 305
            L+ +   WL ++P++++ L+RT+ENIKDP++R FEREV  G  LL  VR+DL DV +VC+G KK TN+ R++
Sbjct: 4380 LHTTASNWLHLIPQTLSHLKRTVENIKDPLFRFFEREVKMGAKLLQDVRQDLADVVQVCEGKKKQTNYLRTL 4451
[Close]
BLAST of SMED30020566 vs. Ensembl Human
Match: DYNC1H1 (dynein cytoplasmic 1 heavy chain 1 [Source:HGNC Symbol;Acc:HGNC:2961])

HSP 1 Score: 87.4261 bits (215), Expect = 1.548e-19
Identity = 36/72 (50.00%), Postives = 54/72 (75.00%), Query Frame = -3 
Query:   90 LYQSTQTWLQILPKSVTPLQRTIENIKDPMYRCFEREVNSGCNLLASVRKDLEDVAEVCKGAKKPTNHHRSM 305
            L+ +   WL ++P++++ L+RT+ENIKDP++R FEREV  G  LL  VR+DL DV +VC+G KK TN+ R++
Sbjct: 1676 LHTTASNWLHLIPQTLSHLKRTVENIKDPLFRFFEREVKMGAKLLQDVRQDLADVVQVCEGKKKQTNYLRTL 1747
[Close]
BLAST of SMED30020566 vs. Ensembl Human
Match: DYNC1H1 (dynein cytoplasmic 1 heavy chain 1 [Source:HGNC Symbol;Acc:HGNC:2961])

HSP 1 Score: 87.0409 bits (214), Expect = 2.100e-19
Identity = 36/72 (50.00%), Postives = 54/72 (75.00%), Query Frame = -3 
Query:   90 LYQSTQTWLQILPKSVTPLQRTIENIKDPMYRCFEREVNSGCNLLASVRKDLEDVAEVCKGAKKPTNHHRSM 305
            L+ +   WL ++P++++ L+RT+ENIKDP++R FEREV  G  LL  VR+DL DV +VC+G KK TN+ R++
Sbjct:  784 LHTTASNWLHLIPQTLSHLKRTVENIKDPLFRFFEREVKMGAKLLQDVRQDLADVVQVCEGKKKQTNYLRTL 855
[Close]
BLAST of SMED30020566 vs. Ensembl Celegans
Match: dhc-1 (Dynein heavy chain, cytoplasmic [Source:UniProtKB/Swiss-Prot;Acc:Q19020])

HSP 1 Score: 83.5741 bits (205), Expect = 1.132e-18
Identity = 33/65 (50.77%), Postives = 48/65 (73.85%), Query Frame = -3 
Query:   90 WLQILPKSVTPLQRTIENIKDPMYRCFEREVNSGCNLLASVRKDLEDVAEVCKGAKKPTNHHRSM 284
            WLQ+LPK +  ++RT+ENIKDP++R FEREVN G  LL  +R+DL +++ VC+  KK  N  R++
Sbjct: 4324 WLQLLPKEIVKMRRTVENIKDPLFRFFEREVNLGSQLLKDIRRDLNEISAVCRAEKKQNNETRAL 4388
[Close]
BLAST of SMED30020566 vs. Ensembl Celegans
Match: dhc-1 (Dynein heavy chain, cytoplasmic [Source:UniProtKB/Swiss-Prot;Acc:Q19020])

HSP 1 Score: 83.5741 bits (205), Expect = 1.132e-18
Identity = 33/65 (50.77%), Postives = 48/65 (73.85%), Query Frame = -3 
Query:   90 WLQILPKSVTPLQRTIENIKDPMYRCFEREVNSGCNLLASVRKDLEDVAEVCKGAKKPTNHHRSM 284
            WLQ+LPK +  ++RT+ENIKDP++R FEREVN G  LL  +R+DL +++ VC+  KK  N  R++
Sbjct: 4324 WLQLLPKEIVKMRRTVENIKDPLFRFFEREVNLGSQLLKDIRRDLNEISAVCRAEKKQNNETRAL 4388
[Close]
BLAST of SMED30020566 vs. Ensembl Celegans
Match: dhc-1 (Dynein heavy chain, cytoplasmic [Source:UniProtKB/Swiss-Prot;Acc:Q19020])

HSP 1 Score: 83.5741 bits (205), Expect = 1.236e-18
Identity = 33/65 (50.77%), Postives = 48/65 (73.85%), Query Frame = -3 
Query:   90 WLQILPKSVTPLQRTIENIKDPMYRCFEREVNSGCNLLASVRKDLEDVAEVCKGAKKPTNHHRSM 284
            WLQ+LPK +  ++RT+ENIKDP++R FEREVN G  LL  +R+DL +++ VC+  KK  N  R++
Sbjct: 3392 WLQLLPKEIVKMRRTVENIKDPLFRFFEREVNLGSQLLKDIRRDLNEISAVCRAEKKQNNETRAL 3456
[Close]
BLAST of SMED30020566 vs. Ensembl Fly
Match: Dhc64C (gene:FBgn0261797 transcript:FBtr0332746)

HSP 1 Score: 97.4413 bits (241), Expect = 2.453e-23
Identity = 43/72 (59.72%), Postives = 55/72 (76.39%), Query Frame = -3 
Query:   90 LYQSTQTWLQILPKSVTPLQRTIENIKDPMYRCFEREVNSGCNLLASVRKDLEDVAEVCKGAKKPTNHHRSM 305
            L+ S   WL++LPK++  L+RT+ENIKDP+YR FEREV SG  LL +V  DL+DV  +C+G KK TNHHRSM
Sbjct: 4378 LHNSATAWLELLPKNLQVLKRTVENIKDPLYRYFEREVTSGSRLLQTVILDLQDVVLICQGEKKQTNHHRSM 4449
[Close]
BLAST of SMED30020566 vs. Ensembl Fly
Match: Dhc64C (gene:FBgn0261797 transcript:FBtr0332745)

HSP 1 Score: 97.4413 bits (241), Expect = 2.501e-23
Identity = 43/72 (59.72%), Postives = 55/72 (76.39%), Query Frame = -3 
Query:   90 LYQSTQTWLQILPKSVTPLQRTIENIKDPMYRCFEREVNSGCNLLASVRKDLEDVAEVCKGAKKPTNHHRSM 305
            L+ S   WL++LPK++  L+RT+ENIKDP+YR FEREV SG  LL +V  DL+DV  +C+G KK TNHHRSM
Sbjct: 4382 LHNSATAWLELLPKNLQVLKRTVENIKDPLYRYFEREVTSGSRLLQTVILDLQDVVLICQGEKKQTNHHRSM 4453
[Close]
BLAST of SMED30020566 vs. Ensembl Fly
Match: Dhc64C (gene:FBgn0261797 transcript:FBtr0332749)

HSP 1 Score: 97.4413 bits (241), Expect = 2.501e-23
Identity = 43/72 (59.72%), Postives = 55/72 (76.39%), Query Frame = -3 
Query:   90 LYQSTQTWLQILPKSVTPLQRTIENIKDPMYRCFEREVNSGCNLLASVRKDLEDVAEVCKGAKKPTNHHRSM 305
            L+ S   WL++LPK++  L+RT+ENIKDP+YR FEREV SG  LL +V  DL+DV  +C+G KK TNHHRSM
Sbjct: 4391 LHNSATAWLELLPKNLQVLKRTVENIKDPLYRYFEREVTSGSRLLQTVILDLQDVVLICQGEKKQTNHHRSM 4462
[Close]
BLAST of SMED30020566 vs. Ensembl Fly
Match: Dhc64C (gene:FBgn0261797 transcript:FBtr0273370)

HSP 1 Score: 97.4413 bits (241), Expect = 2.525e-23
Identity = 43/72 (59.72%), Postives = 55/72 (76.39%), Query Frame = -3 
Query:   90 LYQSTQTWLQILPKSVTPLQRTIENIKDPMYRCFEREVNSGCNLLASVRKDLEDVAEVCKGAKKPTNHHRSM 305
            L+ S   WL++LPK++  L+RT+ENIKDP+YR FEREV SG  LL +V  DL+DV  +C+G KK TNHHRSM
Sbjct: 4369 LHNSATAWLELLPKNLQVLKRTVENIKDPLYRYFEREVTSGSRLLQTVILDLQDVVLICQGEKKQTNHHRSM 4440
[Close]
BLAST of SMED30020566 vs. Ensembl Fly
Match: Dhc64C (gene:FBgn0261797 transcript:FBtr0332748)

HSP 1 Score: 97.4413 bits (241), Expect = 2.525e-23
Identity = 43/72 (59.72%), Postives = 55/72 (76.39%), Query Frame = -3 
Query:   90 LYQSTQTWLQILPKSVTPLQRTIENIKDPMYRCFEREVNSGCNLLASVRKDLEDVAEVCKGAKKPTNHHRSM 305
            L+ S   WL++LPK++  L+RT+ENIKDP+YR FEREV SG  LL +V  DL+DV  +C+G KK TNHHRSM
Sbjct: 4379 LHNSATAWLELLPKNLQVLKRTVENIKDPLYRYFEREVTSGSRLLQTVILDLQDVVLICQGEKKQTNHHRSM 4450
[Close]
BLAST of SMED30020566 vs. Ensembl Xenopus
Match: thumpd2 (THUMP domain containing 2 [Source:Xenbase;Acc:XB-GENE-965047])

HSP 1 Score: 87.4261 bits (215), Expect = 1.434e-19
Identity = 36/65 (55.38%), Postives = 51/65 (78.46%), Query Frame = -3 
Query:   90 WLQILPKSVTPLQRTIENIKDPMYRCFEREVNSGCNLLASVRKDLEDVAEVCKGAKKPTNHHRSM 284
            WLQ++P+++  L+RT+ENIKDP++R FEREV  G  LL  VR+DL DV +VC+G KK TN+ R++
Sbjct: 4378 WLQVIPQALNHLKRTVENIKDPLFRFFEREVKMGAKLLHDVRQDLTDVVQVCEGKKKQTNYLRTL 4442
[Close]
BLAST of SMED30020566 vs. Ensembl Xenopus
Match: thumpd2 (THUMP domain containing 2 [Source:Xenbase;Acc:XB-GENE-965047])

HSP 1 Score: 87.4261 bits (215), Expect = 1.434e-19
Identity = 36/65 (55.38%), Postives = 51/65 (78.46%), Query Frame = -3 
Query:   90 WLQILPKSVTPLQRTIENIKDPMYRCFEREVNSGCNLLASVRKDLEDVAEVCKGAKKPTNHHRSM 284
            WLQ++P+++  L+RT+ENIKDP++R FEREV  G  LL  VR+DL DV +VC+G KK TN+ R++
Sbjct: 4378 WLQVIPQALNHLKRTVENIKDPLFRFFEREVKMGAKLLHDVRQDLTDVVQVCEGKKKQTNYLRTL 4442
[Close]
BLAST of SMED30020566 vs. Ensembl Mouse
Match: Dync1h1 (dynein cytoplasmic 1 heavy chain 1 [Source:MGI Symbol;Acc:MGI:103147])

HSP 1 Score: 91.6633 bits (226), Expect = 3.337e-21
Identity = 37/72 (51.39%), Postives = 55/72 (76.39%), Query Frame = -3 
Query:   90 LYQSTQTWLQILPKSVTPLQRTIENIKDPMYRCFEREVNSGCNLLASVRKDLEDVAEVCKGAKKPTNHHRSM 305
            L+ +   WL ++P++++PL+RT+ENIKDP++R FEREV  G  LL  VR+DL DV +VC+G KK TN+ R++
Sbjct: 4378 LHTTASNWLHLIPQTLSPLKRTVENIKDPLFRFFEREVKMGAKLLQDVRQDLADVVQVCEGKKKQTNYLRTL 4449
[Close]
BLAST of SMED30020566 vs. UniProt/SwissProt
Match: sp|P37276|DYHC_DROME (Dynein heavy chain, cytoplasmic OS=Drosophila melanogaster OX=7227 GN=Dhc64C PE=2 SV=2)

HSP 1 Score: 97.4413 bits (241), Expect = 2.605e-22
Identity = 43/72 (59.72%), Postives = 55/72 (76.39%), Query Frame = -3 
Query:   90 LYQSTQTWLQILPKSVTPLQRTIENIKDPMYRCFEREVNSGCNLLASVRKDLEDVAEVCKGAKKPTNHHRSM 305
            L+ S   WL++LPK++  L+RT+ENIKDP+YR FEREV SG  LL +V  DL+DV  +C+G KK TNHHRSM
Sbjct: 4370 LHNSATAWLELLPKNLQVLKRTVENIKDPLYRYFEREVTSGSRLLQTVILDLQDVVLICQGEKKQTNHHRSM 4441
[Close]
BLAST of SMED30020566 vs. UniProt/SwissProt
Match: sp|P38650|DYHC1_RAT (Cytoplasmic dynein 1 heavy chain 1 OS=Rattus norvegicus OX=10116 GN=Dync1h1 PE=1 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 2.312e-20
Identity = 37/72 (51.39%), Postives = 55/72 (76.39%), Query Frame = -3 
Query:   90 LYQSTQTWLQILPKSVTPLQRTIENIKDPMYRCFEREVNSGCNLLASVRKDLEDVAEVCKGAKKPTNHHRSM 305
            L+ +   WL ++P++++PL+RT+ENIKDP++R FEREV  G  LL  VR+DL DV +VC+G KK TN+ R++
Sbjct: 4378 LHTTASNWLHLIPQTLSPLKRTVENIKDPLFRFFEREVKMGAKLLQDVRQDLADVVQVCEGKKKQTNYLRTL 4449
[Close]
BLAST of SMED30020566 vs. UniProt/SwissProt
Match: sp|Q9JHU4|DYHC1_MOUSE (Cytoplasmic dynein 1 heavy chain 1 OS=Mus musculus OX=10090 GN=Dync1h1 PE=1 SV=2)

HSP 1 Score: 91.6633 bits (226), Expect = 2.334e-20
Identity = 37/72 (51.39%), Postives = 55/72 (76.39%), Query Frame = -3 
Query:   90 LYQSTQTWLQILPKSVTPLQRTIENIKDPMYRCFEREVNSGCNLLASVRKDLEDVAEVCKGAKKPTNHHRSM 305
            L+ +   WL ++P++++PL+RT+ENIKDP++R FEREV  G  LL  VR+DL DV +VC+G KK TN+ R++
Sbjct: 4378 LHTTASNWLHLIPQTLSPLKRTVENIKDPLFRFFEREVKMGAKLLQDVRQDLADVVQVCEGKKKQTNYLRTL 4449
[Close]
BLAST of SMED30020566 vs. UniProt/SwissProt
Match: sp|Q14204|DYHC1_HUMAN (Cytoplasmic dynein 1 heavy chain 1 OS=Homo sapiens OX=9606 GN=DYNC1H1 PE=1 SV=5)

HSP 1 Score: 87.8113 bits (216), Expect = 5.590e-19
Identity = 36/72 (50.00%), Postives = 54/72 (75.00%), Query Frame = -3 
Query:   90 LYQSTQTWLQILPKSVTPLQRTIENIKDPMYRCFEREVNSGCNLLASVRKDLEDVAEVCKGAKKPTNHHRSM 305
            L+ +   WL ++P++++ L+RT+ENIKDP++R FEREV  G  LL  VR+DL DV +VC+G KK TN+ R++
Sbjct: 4380 LHTTASNWLHLIPQTLSHLKRTVENIKDPLFRFFEREVKMGAKLLQDVRQDLADVVQVCEGKKKQTNYLRTL 4451
[Close]
BLAST of SMED30020566 vs. UniProt/SwissProt
Match: sp|Q19020|DYHC_CAEEL (Dynein heavy chain, cytoplasmic OS=Caenorhabditis elegans OX=6239 GN=dhc-1 PE=1 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 1.439e-17
Identity = 33/65 (50.77%), Postives = 48/65 (73.85%), Query Frame = -3 
Query:   90 WLQILPKSVTPLQRTIENIKDPMYRCFEREVNSGCNLLASVRKDLEDVAEVCKGAKKPTNHHRSM 284
            WLQ+LPK +  ++RT+ENIKDP++R FEREVN G  LL  +R+DL +++ VC+  KK  N  R++
Sbjct: 4324 WLQLLPKEIVKMRRTVENIKDPLFRFFEREVNLGSQLLKDIRRDLNEISAVCRAEKKQNNETRAL 4388
[Close]
BLAST of SMED30020566 vs. TrEMBL
Match: G7Y725 (Dynein heavy chain 1 cytosolic OS=Clonorchis sinensis OX=79923 GN=CLF_102002 PE=4 SV=1)

HSP 1 Score: 120.168 bits (300), Expect = 8.515e-28
Identity = 66/151 (43.71%), Postives = 89/151 (58.94%), Query Frame = -3 
Query:   90 EKVLLTNLAARLKKDVLTLVATDEAATEDAKQEK-GQSLIGNILKMQQSL--EXXXXXXXXXXXXXXXXXXXXXXXXXXLYQSTQTWLQILPKSVTPLQRTIENIKDPMYRCFEREVNSGCNLLASVRKDLEDVAEVCKGAKKPTNHHRSM 533
            EKVLLTNLA   ++++  LVA ++  +E  K E  GQ + GN+LK+Q++   +                          L  S   WL  LPK+V  L RT+EN++DP+YR FEREVNSG NLLASVR+DLE ++ VC G +KPTN +R M
Sbjct: 4084 EKVLLTNLAITFEQNIHKLVALEQEDSEAGKPEPFGQVMTGNLLKLQEAAADDESLPISAFDRKKSSVVSDARPPWMRQLLGSASAWLSSLPKTVKRLTRTVENLRDPLYRFFEREVNSGSNLLASVRQDLEVISAVCAGERKPTNRYRLM 4234
[Close]
BLAST of SMED30020566 vs. TrEMBL
Match: A0A4S2M0W7 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_003509 PE=4 SV=1)

HSP 1 Score: 117.857 bits (294), Expect = 6.977e-27
Identity = 65/151 (43.05%), Postives = 89/151 (58.94%), Query Frame = -3 
Query:   90 EKVLLTNLAARLKKDVLTLVATDEAATEDAKQEK-GQSLIGNILKMQQSL--EXXXXXXXXXXXXXXXXXXXXXXXXXXLYQSTQTWLQILPKSVTPLQRTIENIKDPMYRCFEREVNSGCNLLASVRKDLEDVAEVCKGAKKPTNHHRSM 533
            EKVLLTNLA   ++++  L+A ++  +E  K E  GQ + GN+LK+Q++   +                          L  S   WL  LPK+V  L RT+EN++DP+YR FEREVNSG NLLASVR+DLE ++ VC G +KPTN +R M
Sbjct: 4401 EKVLLTNLAITCEQNIRKLLALEQEDSEAGKPEPFGQVMTGNLLKLQEAAADDESLPISAFDRKKSSVVSDARPPWMRQLLGSASAWLSSLPKTVKRLTRTVENLRDPLYRFFEREVNSGSNLLASVRQDLEVISAVCAGERKPTNRYRLM 4551
[Close]
BLAST of SMED30020566 vs. TrEMBL
Match: A0A183AC47 (Uncharacterized protein OS=Echinostoma caproni OX=27848 GN=ECPE_LOCUS4532 PE=4 SV=1)

HSP 1 Score: 114.39 bits (285), Expect = 8.842e-26
Identity = 61/151 (40.40%), Postives = 85/151 (56.29%), Query Frame = -3 
Query:   96 EKVLLTNLAARLKKDVLTLVATDEAATEDAKQ--EKGQSLIGNILKMQQSL---EXXXXXXXXXXXXXXXXXXXXXXXXXXLYQSTQTWLQILPKSVTPLQRTIENIKDPMYRCFEREVNSGCNLLASVRKDLEDVAEVCKGAKKPTNHHR 533
            EKVLLTNLA   ++D+  L+A D     D KQ    G  + GN+LK+Q+++   +                          L  +  +WL +LP+S+  L RT +N++DP+YR FEREVNSG NLL +VR+DLE V  +C G +KPTN HR
Sbjct: 3729 EKVLLTNLAMFFERDLRKLIALDLEDDADHKQLAGAGHEMTGNLLKLQEAVTDDDSLVSAGVLDRKKSSVVADSRPVWMKQLQSTASSWLSLLPQSLKRLTRTTDNLRDPLYRFFEREVNSGANLLITVRQDLETVIAICSGERKPTNRHR 3879
[Close]
BLAST of SMED30020566 vs. TrEMBL
Match: A0A564YGD2 (Uncharacterized protein OS=Hymenolepis diminuta OX=6216 GN=WMSIL1_LOCUS5729 PE=4 SV=1)

HSP 1 Score: 114.39 bits (285), Expect = 1.016e-25
Identity = 61/155 (39.35%), Postives = 84/155 (54.19%), Query Frame = -3 
Query:   90 EKVLLTNLAARLKKDVLTLVATDEAATEDAKQEKGQSLIGNILKMQQSLEXXXXXXXXXXXXXXXXXXXXX-------XXXXXLYQSTQTWLQILPKSVTPLQRTIENIKDPMYRCFEREVNSGCNLLASVRKDLEDVAEVCKGAKKPTNHHRSM 533
            EKVLLTN+A   +  +L  +  +    EDA  EKGQ +IGN+LKMQQ++                                  L  S  TW  +LP+ + PL+RT ENI DP++RCF+RE+N+G  LL +VR DL +V +VC G  KP N+HR +
Sbjct: 4221 EKVLLTNMARNFQAHLLRFITVE---AEDAN-EKGQEVIGNLLKMQQAVSTDDVLSGSEMAATTLVKRASVEEGDTRPTWMRQLQASADTWKSLLPERLRPLERTTENIADPLFRCFDREINAGTTLLHTVRDDLTNVIKVCSGTIKPINYHREL 4371
[Close]
BLAST of SMED30020566 vs. TrEMBL
Match: A0A0L8I729 (Uncharacterized protein OS=Octopus bimaculoides OX=37653 GN=OCBIM_22033392mg PE=4 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 5.425e-24
Identity = 46/72 (63.89%), Postives = 58/72 (80.56%), Query Frame = -3 
Query:   90 LYQSTQTWLQILPKSVTPLQRTIENIKDPMYRCFEREVNSGCNLLASVRKDLEDVAEVCKGAKKPTNHHRSM 305
            L +S   WLQ++PKS+  L+RT+ENIKDP++R FEREVN+GC LL+ VR+DL D+  VC G KKPTNHHRSM
Sbjct: 4307 LQKSVSGWLQLVPKSLGSLKRTMENIKDPLFRFFEREVNAGCRLLSDVRQDLTDIMMVCSGEKKPTNHHRSM 4378
[Close]
BLAST of SMED30020566 vs. Ensembl Cavefish
Match: DYNC1H1 (dynein cytoplasmic 1 heavy chain 1 [Source:HGNC Symbol;Acc:HGNC:2961])

HSP 1 Score: 90.1225 bits (222), Expect = 9.061e-21
Identity = 39/72 (54.17%), Postives = 53/72 (73.61%), Query Frame = -3 
Query:   90 LYQSTQTWLQILPKSVTPLQRTIENIKDPMYRCFEREVNSGCNLLASVRKDLEDVAEVCKGAKKPTNHHRSM 305
            L+ +   WLQ++P SV PL+RT+ENIKDP++R FEREV  G  +L  VR+DL DV +VC+G KK TN  R++
Sbjct: 3464 LHTTACNWLQLIPLSVNPLKRTVENIKDPLFRFFEREVKMGARMLQDVRQDLTDVVQVCQGKKKQTNDLRTL 3535
[Close]
BLAST of SMED30020566 vs. Ensembl Nematostella
Match: EDO28787 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T5H6])

HSP 1 Score: 88.5817 bits (218), Expect = 9.980e-21
Identity = 37/72 (51.39%), Postives = 53/72 (73.61%), Query Frame = -3 
Query:   90 LYQSTQTWLQILPKSVTPLQRTIENIKDPMYRCFEREVNSGCNLLASVRKDLEDVAEVCKGAKKPTNHHRSM 305
            L+ +   WL ++PKS+T L+RT++NIKDP++R FEREVN+G  LL +V +DL D   VC+  KKPTN+ R +
Sbjct:  696 LHTTAANWLTMIPKSLTGLKRTLDNIKDPLFRYFEREVNTGIQLLNTVHQDLSDTVLVCEAQKKPTNYLRGL 767
[Close]
BLAST of SMED30020566 vs. Ensembl Nematostella
Match: EDO43994 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RXA1])

HSP 1 Score: 88.5817 bits (218), Expect = 1.405e-20
Identity = 45/117 (38.46%), Postives = 65/117 (55.56%), Query Frame = -3 
Query:   90 EKGQSLIGNILKMQQ-SLEXXXXXXXXXXXXXXXXXXXXXXXXXXLYQSTQTWLQILPKSVTPLQRTIENIKDPMYRCFEREVNSGCNLLASVRKDLEDVAEVCKGAKKPTNHHRSM 437
            ++GQ LI  +LKMQ    +                          L+ +   WL ++PKS+T L+RT++NIKDP++R FEREVN+G  LL +V +DL D   VC+  KKPTN+ R +
Sbjct: 4336 QRGQDLIAKLLKMQLLGDDDELAYSPDATRQKRRESDPRPAWMRTLHTTAANWLTMIPKSLTGLKRTLDNIKDPLFRYFEREVNTGIQLLNTVHQDLSDTVLVCEAQKKPTNYLRGL 4452
[Close]
BLAST of SMED30020566 vs. Ensembl Medaka
Match: ENSORLT00000042889.1 (pep primary_assembly:ASM223467v1:22:6677116:6680253:1 gene:ENSORLG00000026696.1 transcript:ENSORLT00000042889.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 90.8929 bits (224), Expect = 8.234e-23
Identity = 38/72 (52.78%), Postives = 55/72 (76.39%), Query Frame = -3 
Query:   90 LYQSTQTWLQILPKSVTPLQRTIENIKDPMYRCFEREVNSGCNLLASVRKDLEDVAEVCKGAKKPTNHHRSM 305
            L+ ++  WLQ++P++V PL+RT+ENIKDP++R FEREV  G  +L  VR+DL DV +VC+G KK TN  R++
Sbjct:   41 LHTTSCNWLQLIPQTVNPLKRTVENIKDPLFRFFEREVKMGGKMLQEVRQDLSDVVQVCEGKKKQTNDLRTL 112
[Close]
BLAST of SMED30020566 vs. Planmine SMEST
Match: SMESG000038602.1 (SMESG000038602.1)

HSP 1 Score: 238.039 bits (606), Expect = 2.136e-72
Identity = 122/150 (81.33%), Postives = 122/150 (81.33%), Query Frame = -3 
Query:   90 EKVLLTNLAARLKKDVLTLVATDEAATEDAKQEKGQSLIGNILKMQQSLEXXXXXXXXXXXXXXXXXXXXX--XXXXXLYQSTQTWLQILPKSVTPLQRTIENIKDPMYRCFEREVNSGCNLLASVRKDLEDVAEVCKGAKKPTNHHRSM 533
            EKVLLTNLAARLKKDVLTLVATDEAATEDAKQEKGQSLIGNILKMQQSLE                            LYQSTQTWLQILPKSVTPLQRTIENIKDPMYRCFEREVNSGCNLLASVRKDLEDVAEVCKGAKKPTNHHRSM
Sbjct: 3763 EKVLLTNLAARLKKDVLTLVATDEAATEDAKQEKGQSLIGNILKMQQSLEDDEELVVGQSKTKRTIEVGDGRPAWMRQLYQSTQTWLQILPKSVTPLQRTIENIKDPMYRCFEREVNSGCNLLASVRKDLEDVAEVCKGAKKPTNHHRSM 3912
[Close]
BLAST of SMED30020566 vs. Planmine SMEST
Match: SMESG000038602.1 (SMESG000038602.1)

HSP 1 Score: 176.407 bits (446), Expect = 7.299e-51
Identity = 88/116 (75.86%), Postives = 88/116 (75.86%), Query Frame = -3 
Query:   90 GQSLIGNILKMQQSLEXXXXXXXXXXXXXXXXXXXXX--XXXXXLYQSTQTWLQILPKSVTPLQRTIENIKDPMYRCFEREVNSGCNLLASVRKDLEDVAEVCKGAKKPTNHHRSM 431
            GQSLIGNILKMQQSLE                            LYQSTQTWLQILPKSVTPLQRTIENIKDPMYRCFEREVNSGCNLLASVRKDLEDVAEVCKGAKKPTNHHRSM
Sbjct: 3771 GQSLIGNILKMQQSLEDDEELVVGQSKTKRTIEVGDGRPAWMRQLYQSTQTWLQILPKSVTPLQRTIENIKDPMYRCFEREVNSGCNLLASVRKDLEDVAEVCKGAKKPTNHHRSM 3886
[Close]
BLAST of SMED30020566 vs. Planmine SMEST
Match: SMESG000064358.1 (SMESG000064358.1)

HSP 1 Score: 129.413 bits (324), Expect = 1.453e-34
Identity = 60/116 (51.72%), Postives = 74/116 (63.79%), Query Frame = -3 
Query:   90 GQSLIGNILKMQQSLEXXXXXXXXXXXXXXXXXXXXX--XXXXXLYQSTQTWLQILPKSVTPLQRTIENIKDPMYRCFEREVNSGCNLLASVRKDLEDVAEVCKGAKKPTNHHRSM 431
            G SLIGNILK+ QSLE                            L+Q+TQ WL +LP+S+ PL+RT +NIKDP+YRCFERE+  GC LL  VR+DLED+AE+C   KKPTNHHRS+
Sbjct: 4337 GNSLIGNILKLHQSLENDEEIMVDQNKSQQKIRHGNGQPTWMQQLFQTTQEWLHLLPRSIVPLRRTTDNIKDPIYRCFEREIILGCKLLVEVRQDLEDIAEICNALKKPTNHHRSL 4452
The following BLAST results are available for this feature:
BLAST of SMED30020566 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
DYNC1H15.501e-2050.00dynein cytoplasmic 1 heavy chain 1 [Source:HGNC Sy... [more]
DYNC1H11.135e-1950.00dynein cytoplasmic 1 heavy chain 1 [Source:HGNC Sy... [more]
DYNC1H11.164e-1950.00dynein cytoplasmic 1 heavy chain 1 [Source:HGNC Sy... [more]
DYNC1H11.548e-1950.00dynein cytoplasmic 1 heavy chain 1 [Source:HGNC Sy... [more]
DYNC1H12.100e-1950.00dynein cytoplasmic 1 heavy chain 1 [Source:HGNC Sy... [more]
back to top
BLAST of SMED30020566 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 3
Match NameE-valueIdentityDescription
dhc-11.132e-1850.77Dynein heavy chain, cytoplasmic [Source:UniProtKB... [more]
dhc-11.132e-1850.77Dynein heavy chain, cytoplasmic [Source:UniProtKB... [more]
dhc-11.236e-1850.77Dynein heavy chain, cytoplasmic [Source:UniProtKB... [more]
back to top
BLAST of SMED30020566 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Dhc64C2.453e-2359.72gene:FBgn0261797 transcript:FBtr0332746[more]
Dhc64C2.501e-2359.72gene:FBgn0261797 transcript:FBtr0332745[more]
Dhc64C2.501e-2359.72gene:FBgn0261797 transcript:FBtr0332749[more]
Dhc64C2.525e-2359.72gene:FBgn0261797 transcript:FBtr0273370[more]
Dhc64C2.525e-2359.72gene:FBgn0261797 transcript:FBtr0332748[more]
back to top
BLAST of SMED30020566 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30020566 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
thumpd21.434e-1955.38THUMP domain containing 2 [Source:Xenbase;Acc:XB-G... [more]
thumpd21.434e-1955.38THUMP domain containing 2 [Source:Xenbase;Acc:XB-G... [more]
back to top
BLAST of SMED30020566 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Dync1h13.337e-2151.39dynein cytoplasmic 1 heavy chain 1 [Source:MGI Sym... [more]
back to top
BLAST of SMED30020566 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P37276|DYHC_DROME2.605e-2259.72Dynein heavy chain, cytoplasmic OS=Drosophila mela... [more]
sp|P38650|DYHC1_RAT2.312e-2051.39Cytoplasmic dynein 1 heavy chain 1 OS=Rattus norve... [more]
sp|Q9JHU4|DYHC1_MOUSE2.334e-2051.39Cytoplasmic dynein 1 heavy chain 1 OS=Mus musculus... [more]
sp|Q14204|DYHC1_HUMAN5.590e-1950.00Cytoplasmic dynein 1 heavy chain 1 OS=Homo sapiens... [more]
sp|Q19020|DYHC_CAEEL1.439e-1750.77Dynein heavy chain, cytoplasmic OS=Caenorhabditis ... [more]
back to top
BLAST of SMED30020566 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
G7Y7258.515e-2843.71Dynein heavy chain 1 cytosolic OS=Clonorchis sinen... [more]
A0A4S2M0W76.977e-2743.05Uncharacterized protein OS=Opisthorchis felineus O... [more]
A0A183AC478.842e-2640.40Uncharacterized protein OS=Echinostoma caproni OX=... [more]
A0A564YGD21.016e-2539.35Uncharacterized protein OS=Hymenolepis diminuta OX... [more]
A0A0L8I7295.425e-2463.89Uncharacterized protein OS=Octopus bimaculoides OX... [more]
back to top
BLAST of SMED30020566 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
DYNC1H19.061e-2154.17dynein cytoplasmic 1 heavy chain 1 [Source:HGNC Sy... [more]
back to top
BLAST of SMED30020566 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30020566 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30020566 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2
Match NameE-valueIdentityDescription
EDO287879.980e-2151.39Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO439941.405e-2038.46Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
back to top
BLAST of SMED30020566 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSORLT00000042889.18.234e-2352.78pep primary_assembly:ASM223467v1:22:6677116:668025... [more]
back to top
BLAST of SMED30020566 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000038602.12.136e-7281.33SMESG000038602.1[more]
SMESG000038602.17.299e-5175.86SMESG000038602.1[more]
SMESG000064358.11.453e-3451.72SMESG000064358.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30020566 ID=SMED30020566|Name=SMED30020566|organism=Schmidtea mediterranea sexual|type=transcript|length=595bp
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCATCGATCTAT
GATGATTGGTCGGTTTCTTGGCTCCTTTACAGACTTCAGCAACGTCTTCA
AGATCTTTTCGAACAGAGGCTAATAAATTACATCCCGAATTAACTTCTCT
TTCAAAACATCGATACATAGGATCTTTTATATTTTCGATGGTTCGTTGAA
GAGGTGTTACAGATTTCGGTAATATTTGTAGCCATGTTTGAGTGCTTTGA
TATAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCTTCCAATGACTGTTGCA
TTTTCAGAATATTTCCAATTAATGATTGTCCTTTTTCTTGTTTAGCGTCC
TCTGTTGCTGCTTCGTCGGTAGCAACCAGGGTTAAAACATCTTTTTTTAA
TCTTGCAGCTAAGTTAGTCAACAAAACTTTCTCNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
back to top

protein sequence of SMED30020566-orf-1

>SMED30020566-orf-1 ID=SMED30020566-orf-1|Name=SMED30020566-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=197bp
XXXXXXXXXXXXXXXXXXXXEKVLLTNLAARLKKDVLTLVATDEAATEDA
KQEKGQSLIGNILKMQQSLEXXXXXXXXXXXXXXXXXXXXXXXXXXLYQS
TQTWLQILPKSVTPLQRTIENIKDPMYRCFEREVNSGCNLLASVRKDLED
VAEVCKGAKKPTNHHRSMXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0000418head
PLANA:0000484secretory system
PLANA:0003116parenchymal cell
PLANA:0003117parapharyngeal cell
Vocabulary: INTERPRO
TermDefinition
IPR041228Dynein_C
IPR004273Dynein_heavy_D6_P-loop
Vocabulary: biological process
TermDefinition
GO:0007018microtubule-based movement
Vocabulary: molecular function
TermDefinition
GO:0003777microtubule motor activity
Vocabulary: cellular component
TermDefinition
GO:0030286dynein complex
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:1.20.1270.280coord: 1..72
e-value: 2.0E-28
score: 101.3
NoneNo IPR availablePANTHERPTHR45703:SF14coord: 2..71
NoneNo IPR availablePANTHERPTHR45703FAMILY NOT NAMEDcoord: 2..71
IPR041228Dynein heavy chain, C-terminal domainPFAMPF18199Dynein_Ccoord: 3..71
e-value: 8.5E-8
score: 31.8