Ribonuclease H1

Overview
NameRibonuclease H1
Smed IDSMED30020562
Length (bp)1159
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Ribonuclease H1 (SMED30020562) t-SNE clustered cells

Violin plots show distribution of expression levels for Ribonuclease H1 (SMED30020562) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Ribonuclease H1 (SMED30020562) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Ribonuclease H1 (SMED30020562) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30020562

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30020562SMESG000002048.1 SmedASXL_012102SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30020562SMESG000002048.1 SmedASXL_012102SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
neoblastSMED30020562SMESG000002048.1 dd_Smed_v4_3892_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Ribonuclease H1 vs. Ensembl Human
Match: RNASEH1 (ribonuclease H1 [Source:HGNC Symbol;Acc:HGNC:18466])

HSP 1 Score: 157.918 bits (398), Expect = 6.844e-45
Identity = 106/282 (37.59%), Postives = 141/282 (50.00%), Query Frame = 1 
Query:  103 FYVVINGREPGIYKTWLIGL*FWFLNNSFRPECQRQVDNFPNAKFKGFMTKEEADNYIDISKSGSGKNLFKRKIESDMNLFAPKRVKIDKPTTNPLEFEFCIKQQKESYIKGIKVDLLRDPIEP----------NRVCVYTDGSCRK-LKTGCIAGYGVYWGKNSPWNVGEKYVGVQTNNRAEIAAVISAVETASKNGIDKLRIITDSQFTINCMTKWIGKWKRNNWMTSGNKEAQCRDVLEKLDNLILSTKLDIIWEHVNGHSGNPGNEAADQLANLGADQ 915
            FY V  GR+ G++ TW               EC+ QVD FP A+FK F T++EA  ++  S S       + +   +    A KR++       PL+ +    +  E Y K +K  +  +P  P          + V VYTDG C    +    AG GVYWG   P NVG +  G QTN RAEI A   A+E A    I+KL + TDS FTIN +T W+  WK+N W TS  KE   ++    L+   L+  +DI W HV GHSG  GNE AD+LA  GA Q
Sbjct:   28 FYAVRRGRKTGVFLTW--------------NECRAQVDRFPAARFKKFATEDEAWAFVRKSASPEVSEGHENQHGQESEAKASKRLR------EPLDGDG--HESAEPYAKHMKPSV--EPAPPVSRDTFSYMGDFVVVYTDGCCSSNGRRRPRAGIGVYWGPGHPLNVGIRLPGRQTNQRAEIHAACKAIEQAKTQNINKLVLYTDSMFTINGITNWVQGWKKNGWKTSAGKEVINKEDFVALER--LTQGMDIQWMHVPGHSGFIGNEEADRLAREGAKQ 283
[Close]
BLAST of Ribonuclease H1 vs. Ensembl Human
Match: AC108488.2 (novel protein)

HSP 1 Score: 157.918 bits (398), Expect = 6.844e-45
Identity = 106/282 (37.59%), Postives = 141/282 (50.00%), Query Frame = 1 
Query:  103 FYVVINGREPGIYKTWLIGL*FWFLNNSFRPECQRQVDNFPNAKFKGFMTKEEADNYIDISKSGSGKNLFKRKIESDMNLFAPKRVKIDKPTTNPLEFEFCIKQQKESYIKGIKVDLLRDPIEP----------NRVCVYTDGSCRK-LKTGCIAGYGVYWGKNSPWNVGEKYVGVQTNNRAEIAAVISAVETASKNGIDKLRIITDSQFTINCMTKWIGKWKRNNWMTSGNKEAQCRDVLEKLDNLILSTKLDIIWEHVNGHSGNPGNEAADQLANLGADQ 915
            FY V  GR+ G++ TW               EC+ QVD FP A+FK F T++EA  ++  S S       + +   +    A KR++       PL+ +    +  E Y K +K  +  +P  P          + V VYTDG C    +    AG GVYWG   P NVG +  G QTN RAEI A   A+E A    I+KL + TDS FTIN +T W+  WK+N W TS  KE   ++    L+   L+  +DI W HV GHSG  GNE AD+LA  GA Q
Sbjct:   28 FYAVRRGRKTGVFLTW--------------NECRAQVDRFPAARFKKFATEDEAWAFVRKSASPEVSEGHENQHGQESEAKASKRLR------EPLDGDG--HESAEPYAKHMKPSV--EPAPPVSRDTFSYMGDFVVVYTDGCCSSNGRRRPRAGIGVYWGPGHPLNVGIRLPGRQTNQRAEIHAACKAIEQAKTQNINKLVLYTDSMFTINGITNWVQGWKKNGWKTSAGKEVINKEDFVALER--LTQGMDIQWMHVPGHSGFIGNEEADRLAREGAKQ 283
[Close]
BLAST of Ribonuclease H1 vs. Ensembl Human
Match: AC108488.2 (novel protein)

HSP 1 Score: 157.918 bits (398), Expect = 6.844e-45
Identity = 106/282 (37.59%), Postives = 141/282 (50.00%), Query Frame = 1 
Query:  103 FYVVINGREPGIYKTWLIGL*FWFLNNSFRPECQRQVDNFPNAKFKGFMTKEEADNYIDISKSGSGKNLFKRKIESDMNLFAPKRVKIDKPTTNPLEFEFCIKQQKESYIKGIKVDLLRDPIEP----------NRVCVYTDGSCRK-LKTGCIAGYGVYWGKNSPWNVGEKYVGVQTNNRAEIAAVISAVETASKNGIDKLRIITDSQFTINCMTKWIGKWKRNNWMTSGNKEAQCRDVLEKLDNLILSTKLDIIWEHVNGHSGNPGNEAADQLANLGADQ 915
            FY V  GR+ G++ TW               EC+ QVD FP A+FK F T++EA  ++  S S       + +   +    A KR++       PL+ +    +  E Y K +K  +  +P  P          + V VYTDG C    +    AG GVYWG   P NVG +  G QTN RAEI A   A+E A    I+KL + TDS FTIN +T W+  WK+N W TS  KE   ++    L+   L+  +DI W HV GHSG  GNE AD+LA  GA Q
Sbjct:   28 FYAVRRGRKTGVFLTW--------------NECRAQVDRFPAARFKKFATEDEAWAFVRKSASPEVSEGHENQHGQESEAKASKRLR------EPLDGDG--HESAEPYAKHMKPSV--EPAPPVSRDTFSYMGDFVVVYTDGCCSSNGRRRPRAGIGVYWGPGHPLNVGIRLPGRQTNQRAEIHAACKAIEQAKTQNINKLVLYTDSMFTINGITNWVQGWKKNGWKTSAGKEVINKEDFVALER--LTQGMDIQWMHVPGHSGFIGNEEADRLAREGAKQ 283
[Close]
BLAST of Ribonuclease H1 vs. Ensembl Celegans
Match: rnh-1.0 (Ribonuclease H1 [Source:UniProtKB/TrEMBL;Acc:Q86MG7])

HSP 1 Score: 140.584 bits (353), Expect = 4.228e-39
Identity = 98/281 (34.88%), Postives = 133/281 (47.33%), Query Frame = 1 
Query:  100 KFYVVINGREPGIYKTWLIGL*FWFLNNSFRPECQRQVDNFPNAKFKGFMTKEEADNYIDISKSGSGKNLFKRKIESDMNLFAPKRVKIDKPTTNPLEFEFCIKQQKESYIKGIKVDLLR------DPIEPNRVCVYTDGSCRKLKT-GCIAGYGVYWGKNSPWNVGEKYVGVQTNNRAEIAAVISAVETASKNGIDKLRIITDSQFTINCMTKWIGKWKRNNWMTSGNKEAQCRDVLEKLDNLILSTKLDIIWEHVNGHSGNPGNEAADQLANLGADQWL 921
            ++Y V  G++ GIY+TW               EC+ Q+D F NA+FK F T+ EA                 RK  +D N+  P      KP T  +      +++     K  +   ++      DP   N   VYTDG+C    T    AG+GVYWG +S  N      G  TNNR E+ AV  A+E A +  + K+ I TDS   +  M  WI  WKR  W TS   E   +DVL K+DN  L  KL + + HV GH+G  GNE AD+LA  GA  ++
Sbjct:    6 QYYAVARGKQVGIYRTW--------------NECKTQIDGFQNARFKKFATEAEA-----------------RKFVAD-NMSVPG----SKPVTPAVSTSSATRKRTHEGTKFTEAKKMKTEEEVIDPEFANAPVVYTDGACSSNGTKNAKAGWGVYWGDDSEDNEFGPVYGAPTNNRGELIAVQKAIEKAIEKRLPKVVIKTDSNLLVQSMNIWIHGWKRKGWKTSTGSEVLNQDVLMKIDN--LRQKLKVKFLHVRGHAGIDGNEKADELARKGAQMFI 248
[Close]
BLAST of Ribonuclease H1 vs. Ensembl Celegans
Match: rnh-1.3 (RNase H [Source:UniProtKB/TrEMBL;Acc:Q9XVE6])

HSP 1 Score: 71.2478 bits (173), Expect = 7.133e-15
Identity = 45/129 (34.88%), Postives = 68/129 (52.71%), Query Frame = 1 
Query:  490 YTDGSC-RKLKTGCIAGYGVYWGKNSPWNVGEKYV-GVQTNNRAEIAAVISAVETASKNGIDKLRIITDSQFTINCMTKWIGKWKRNNWMTSGNKEAQCRDVLEKLDNLILSTKLD---IIWEHVNGHS 861
            YTDGS     + G   GYGV+   ++  NV   Y  G QTNNR E+ A+  A + A       +RI TDS+   + +TKW   WK N + TS  ++ + +D++  +D  + + K     + +EHV GHS
Sbjct:    6 YTDGSALGNGQQGSRGGYGVHVPSDTSKNVSGSYPHGPQTNNRYELEAIKHATQMARDVPDSHVRIHTDSKNAKDSVTKWNDNWKSNGYKTSSGQDVKNQDLIRDIDRNVQALKHSGKTVEFEHVRGHS 134
[Close]
BLAST of Ribonuclease H1 vs. Ensembl Celegans
Match: rnh-1.3 (RNase H [Source:UniProtKB/TrEMBL;Acc:Q9XVE6])

HSP 1 Score: 71.2478 bits (173), Expect = 7.133e-15
Identity = 45/129 (34.88%), Postives = 68/129 (52.71%), Query Frame = 1 
Query:  490 YTDGSC-RKLKTGCIAGYGVYWGKNSPWNVGEKYV-GVQTNNRAEIAAVISAVETASKNGIDKLRIITDSQFTINCMTKWIGKWKRNNWMTSGNKEAQCRDVLEKLDNLILSTKLD---IIWEHVNGHS 861
            YTDGS     + G   GYGV+   ++  NV   Y  G QTNNR E+ A+  A + A       +RI TDS+   + +TKW   WK N + TS  ++ + +D++  +D  + + K     + +EHV GHS
Sbjct:    6 YTDGSALGNGQQGSRGGYGVHVPSDTSKNVSGSYPHGPQTNNRYELEAIKHATQMARDVPDSHVRIHTDSKNAKDSVTKWNDNWKSNGYKTSSGQDVKNQDLIRDIDRNVQALKHSGKTVEFEHVRGHS 134
[Close]
BLAST of Ribonuclease H1 vs. Ensembl Celegans
Match: rnh-1.1 (RNase H [Source:UniProtKB/TrEMBL;Acc:Q09633])

HSP 1 Score: 53.9138 bits (128), Expect = 9.693e-8
Identity = 44/154 (28.57%), Postives = 68/154 (44.16%), Query Frame = 1 
Query:  481 VCVYTDGSCRKLKTGCIAGYGVYWGKNSPWNVGEKYVG-VQTNNRAEIAAVISAVETASKN---GIDKLRIITDSQFTINCMTKWIGKWKRNNWMTSGNKEAQCRDVLEKLDNLILSTKLDIIWEHVNGHSGNPGNEAADQLANLGADQWLNEI 930
            + +YTDGS   LK    +G G++ G     N  ++  G +Q NN AE  AV +A++ A KN      K+ I TD    I  +               G +     DV  +++ L       + ++HV  H+G+PGNE AD  A   + +  N  
Sbjct:  327 LVMYTDGSY--LKRPPTSGIGIFVGPGHELNRSQRIRGPIQDNNYAEFIAVRTALQNALKNENYRDQKVVIRTDCLNVIEAL--------------QGTRPTAFVDVKSQVEFLSKQFPKGVHFQHVYAHAGDPGNEMADLFAGQASSKSQNSF 464
[Close]
BLAST of Ribonuclease H1 vs. Ensembl Fly
Match: rnh1 (gene:FBgn0023171 transcript:FBtr0088884)

HSP 1 Score: 135.191 bits (339), Expect = 2.016e-36
Identity = 102/313 (32.59%), Postives = 145/313 (46.33%), Query Frame = 1 
Query:   82 LLKMVKKFYVVINGREPGIYKTWLIGL*FWFLNNSFRPECQRQVDNFPNAKFKGFMTKEEADNYIDISKSGSGKNL-------------FKRKIESDMNLFAPKR-----------VKIDKP----------TTNPLEFEFCIKQQKESYIKGIK-VDLLRDPIEPN-RVCVYTDGSC-RKLKTGCIAGYGVYWGKNSPWNVGEKYVGVQTNNRAEIAAVISAVETASKNGIDKLRIITDSQFTINCMTKWIGKWKRNNWMTSGNKEAQCRDVLEKLDNLILSTKLDIIWEHVNGHSGNPGNEAADQLANLGA 909
            L +    FY V +GR  G+Y +W               EC+ QV  F NAK+K F T++EAD +++  KS + +++             +K  IE + N   PK             + D P          T+     +      + S   G+K V   +  I+    V VYTDGSC    + G  AGYGVY+GKN   N  +   G  TNN  EI A I A++TA   GI KL I TDSQF IN +T W+  WK+ +W    N+  +     ++LD L+    + + W +V  H G  GNE AD+LA  G+
Sbjct:   11 LQRCTMAFYAVASGRRSGVYGSW--------------AECEEQVKGFKNAKYKKFKTRQEADQFVNGCKSYAPQDVAVPLGKEKASLASWKNSIEVNKN---PKYTDEWPEEDHDLAEDDLPDILNRKRKGTTSGDKRNKIPRHASQVSEATGLKQVGAFQFEIDAEGYVIVYTDGSCIGNGRAGACAGYGVYFGKNHQLNAAKPVEGRVTNNVGEIQAAIHAIKTALDLGIQKLCISTDSQFLINSITLWVAGWKKRDWKLKNNQPVKNVVDFKELDKLLQENNITVKWNYVEAHKGIEGNEMADKLARQGS 306
[Close]
BLAST of Ribonuclease H1 vs. Ensembl Fly
Match: rnh1 (gene:FBgn0023171 transcript:FBtr0088883)

HSP 1 Score: 114.39 bits (285), Expect = 1.708e-28
Identity = 63/144 (43.75%), Postives = 82/144 (56.94%), Query Frame = 1 
Query:  481 VCVYTDGSC-RKLKTGCIAGYGVYWGKNSPWNVGEKYVGVQTNNRAEIAAVISAVETASKNGIDKLRIITDSQFTINCMTKWIGKWKRNNWMTSGNKEAQCRDVLEKLDNLILSTKLDIIWEHVNGHSGNPGNEAADQLANLGA 909
            V VYTDGSC    + G  AGYGVY+GKN   N  +   G  TNN  EI A I A++TA   GI KL I TDSQF IN +T W+  WK+ +W    N+  +     ++LD L+    + + W +V  H G  GNE AD+LA  G+
Sbjct:  182 VIVYTDGSCIGNGRAGACAGYGVYFGKNHQLNAAKPVEGRVTNNVGEIQAAIHAIKTALDLGIQKLCISTDSQFLINSITLWVAGWKKRDWKLKNNQPVKNVVDFKELDKLLQENNITVKWNYVEAHKGIEGNEMADKLARQGS 325

HSP 2 Score: 50.447 bits (119), Expect = 1.326e-6
Identity = 25/70 (35.71%), Postives = 36/70 (51.43%), Query Frame = 1 
Query:   82 LLKMVKKFYVVINGREPGIYKTWLIGL*FWFLNNSFRPECQRQVDNFPNAKFKGFMTKEEADNYIDISKS 291
            L +    FY V +GR  G+Y +W               EC+ QV  F NAK+K F T++EAD +++  KS
Sbjct:   11 LQRCTMAFYAVASGRRSGVYGSW--------------AECEEQVKGFKNAKYKKFKTRQEADQFVNGCKS 66
[Close]
BLAST of Ribonuclease H1 vs. Ensembl Zebrafish
Match: rnaseh1 (ribonuclease H1 [Source:ZFIN;Acc:ZDB-GENE-040718-407])

HSP 1 Score: 150.214 bits (378), Expect = 3.014e-42
Identity = 97/284 (34.15%), Postives = 141/284 (49.65%), Query Frame = 1 
Query:  103 FYVVINGREPGIYKTWLIGL*FWFLNNSFRPECQRQVDNFPNAKFKGFMTKEEADNYI---DISKSGSGKNLFKRKIESDMNL--------FAPKRVKIDKPTTNPLEFEFCIKQQKESYIKGIKVDLLRD---PIEPNRVCVYTDGSCR-KLKTGCIAGYGVYWGKNSPWNVGEKYVGVQTNNRAEIAAVISAVETASKNGIDKLRIITDSQFTINCMTKWIGKWKRNNWMTSGNKEAQCRDVLEKLDNLILSTKLDIIWEHVNGHSGNPGNEAADQLANLGA 909
            FY V  G +PG+Y+TW               EC+ QVD FP+A FK F ++++A +++     + S +G N  + ++    N+        F  KR   +     P     C K+ K   +   K             + V VYTDG C    K G  AG GVYWG++ P NV E+  G QTN RAE+ A   A+E A +    K+ + TDS+FTIN +T W+  WK N W          +D  ++LD   L+ +LD++W H+ GH+G  GNE AD+L+  GA
Sbjct:    8 FYAVRKGFKPGVYQTW--------------EECKHQVDKFPSASFKKFASEQDALDFVRSHSDASSSAGANGCELEVLPPRNVPCSSMAVSFGSKRTHDETEDEGPS----CPKRVKLIEVPQPKTGTTSSDGFTYMGDAVVVYTDGCCSGNGKHGARAGIGVYWGRDHPRNVAERLPGRQTNQRAELQAACKALEQAKEMNFKKVVLYTDSKFTINGVTSWVKTWKSNGWRLKSGGVIINKDDFQQLDK--LNAELDVVWMHIPGHAGYTGNEEADRLSREGA 271
[Close]
BLAST of Ribonuclease H1 vs. Ensembl Zebrafish
Match: rnaseh1 (ribonuclease H1 [Source:ZFIN;Acc:ZDB-GENE-040718-407])

HSP 1 Score: 150.214 bits (378), Expect = 3.014e-42
Identity = 97/284 (34.15%), Postives = 141/284 (49.65%), Query Frame = 1 
Query:  103 FYVVINGREPGIYKTWLIGL*FWFLNNSFRPECQRQVDNFPNAKFKGFMTKEEADNYI---DISKSGSGKNLFKRKIESDMNL--------FAPKRVKIDKPTTNPLEFEFCIKQQKESYIKGIKVDLLRD---PIEPNRVCVYTDGSCR-KLKTGCIAGYGVYWGKNSPWNVGEKYVGVQTNNRAEIAAVISAVETASKNGIDKLRIITDSQFTINCMTKWIGKWKRNNWMTSGNKEAQCRDVLEKLDNLILSTKLDIIWEHVNGHSGNPGNEAADQLANLGA 909
            FY V  G +PG+Y+TW               EC+ QVD FP+A FK F ++++A +++     + S +G N  + ++    N+        F  KR   +     P     C K+ K   +   K             + V VYTDG C    K G  AG GVYWG++ P NV E+  G QTN RAE+ A   A+E A +    K+ + TDS+FTIN +T W+  WK N W          +D  ++LD   L+ +LD++W H+ GH+G  GNE AD+L+  GA
Sbjct:    8 FYAVRKGFKPGVYQTW--------------EECKHQVDKFPSASFKKFASEQDALDFVRSHSDASSSAGANGCELEVLPPRNVPCSSMAVSFGSKRTHDETEDEGPS----CPKRVKLIEVPQPKTGTTSSDGFTYMGDAVVVYTDGCCSGNGKHGARAGIGVYWGRDHPRNVAERLPGRQTNQRAELQAACKALEQAKEMNFKKVVLYTDSKFTINGVTSWVKTWKSNGWRLKSGGVIINKDDFQQLDK--LNAELDVVWMHIPGHAGYTGNEEADRLSREGA 271
[Close]
BLAST of Ribonuclease H1 vs. Ensembl Zebrafish
Match: rnaseh1 (ribonuclease H1 [Source:ZFIN;Acc:ZDB-GENE-040718-407])

HSP 1 Score: 120.168 bits (300), Expect = 2.701e-31
Identity = 82/251 (32.67%), Postives = 118/251 (47.01%), Query Frame = 1 
Query:  103 FYVVINGREPGIYKTWLIGL*FWFLNNSFRPECQRQVDNFPNAKFKGFMTKEEADNYI---DISKSGSGKNLFKRKIESDMNL--------FAPKRVKIDKPTTNPLEFEFCIKQQKESYIKGIKVDLLRD---PIEPNRVCVYTDGSCR-KLKTGCIAGYGVYWGKNSPWNVGEKYVGVQTNNRAEIAAVISAVETASKNGIDKLRIITDSQFTINCMTKWIGKWKRNNWMTSGNKEAQCRDVLEKLDNL 810
            FY V  G +PG+Y+TW               EC+ QVD FP+A FK F ++++A +++     + S +G N  + ++    N+        F  KR   +     P     C K+ K   +   K             + V VYTDG C    K G  AG GVYWG++ P NV E+  G QTN RAE+ A   A+E A +    K+ + TDS+FTIN +T W+  WK N W          +D  ++LD L
Sbjct:    8 FYAVRKGFKPGVYQTW--------------EECKHQVDKFPSASFKKFASEQDALDFVRSHSDASSSAGANGCELEVLPPRNVPCSSMAVSFGSKRTHDETEDEGPS----CPKRVKLIEVPQPKTGTTSSDGFTYMGDAVVVYTDGCCSGNGKHGARAGIGVYWGRDHPRNVAERLPGRQTNQRAELQAACKALEQAKEMNFKKVVLYTDSKFTINGVTSWVKTWKSNGWRLKSGGVIINKDDFQQLDKL 240
[Close]
BLAST of Ribonuclease H1 vs. Ensembl Xenopus
Match: rnaseh1 (ribonuclease H1 [Source:NCBI gene;Acc:100124876])

HSP 1 Score: 165.622 bits (418), Expect = 9.582e-48
Identity = 111/298 (37.25%), Postives = 145/298 (48.66%), Query Frame = 1 
Query:   94 VKKFYVVINGREPGIYKTWLIGL*FWFLNNSFRPECQRQVDNFPNAKFKGFMTKEEADNYIDISKSGSGKNLFKRKIESDMNLFAPKRVKIDKPTTNPLEFEFCIKQQK--------------ESYIKGIK-VDLLRDPIEPN--------RVCVYTDGSCRK-LKTGCIAGYGVYWGKNSPWNVGEKYVGVQTNNRAEIAAVISAVETASKNGIDKLRIITDSQFTINCMTKWIGKWKRNNWMTSGNKEAQCRDVLEKLDNLILSTKLDIIWEHVNGHSGNPGNEAADQLANLGADQ 915
            ++ FY V  GR+PG+Y TW               EC+ QVD FP A++K F ++E+A N++             R  ES     +    +  +P T        + QQ+              E+  K  K +DL   P   N         V VYTDG C +  +    AG GVYWG N P N+ EK  G QTN RAEI A   A+E A    I KL + TDS FTIN +TKWI  WKRN W  +  K+   R+  EKLD   L+  LDI W H+ GH+G  GNEAAD+L+  GA +
Sbjct:   29 MRMFYAVRTGRKPGVYNTW--------------DECKEQVDKFPLARYKKFASEEDAWNFV-------------RNQESSSKSSSVDTGETKEPPTQATGLHNVVPQQRAKRPLYKAQSSSTEEAAPKRTKLIDLTELPSSHNGAFKYMGDSVVVYTDGCCSQNGRLKARAGIGVYWGLNHPLNLAEKLEGRQTNQRAEIQAACRALELAKSQNITKLVLYTDSMFTINGITKWIHSWKRNGWKLTTGKKVINREDFEKLDK--LTQGLDIKWMHIPGHAGFEGNEAADRLSREGAQK 297
[Close]
BLAST of Ribonuclease H1 vs. Ensembl Xenopus
Match: rnaseh1 (ribonuclease H1 [Source:NCBI gene;Acc:100124876])

HSP 1 Score: 139.428 bits (350), Expect = 7.083e-38
Identity = 100/280 (35.71%), Postives = 130/280 (46.43%), Query Frame = 1 
Query:   94 VKKFYVVINGREPGIYKTWLIGL*FWFLNNSFRPECQRQVDNFPNAKFKGFMTKEEADNYIDISKSGSGKNLFKRKIESDMNLFAPKRVKIDKPTTNPLEFEFCIKQQK--------------ESYIKGIK-VDLLRDPIEPN--------RVCVYTDGSCRK-LKTGCIAGYGVYWGKNSPWNVGEKYVGVQTNNRAEIAAVISAVETASKNGIDKLRIITDSQFTINCMTKWIGKWKRNNWMTSGNKEAQCRDVLEKLDNLILSTKLDIIWEHV-NGH 858
            ++ FY V  GR+PG+Y TW               EC+ QVD FP A++K F ++E+A N++             R  ES     +    +  +P T        + QQ+              E+  K  K +DL   P   N         V VYTDG C +  +    AG GVYWG N P N+ EK  G QTN RAEI A   A+E A    I KL + TDS FTIN +TKWI  WKRN W  +  K+   R+  EKLD   L+  LDI W  +  GH
Sbjct:   29 MRMFYAVRTGRKPGVYNTW--------------DECKEQVDKFPLARYKKFASEEDAWNFV-------------RNQESSSKSSSVDTGETKEPPTQATGLHNVVPQQRAKRPLYKAQSSSTEEAAPKRTKLIDLTELPSSHNGAFKYMGDSVVVYTDGCCSQNGRLKARAGIGVYWGLNHPLNLAEKLEGRQTNQRAEIQAACRALELAKSQNITKLVLYTDSMFTINGITKWIHSWKRNGWKLTTGKKVINREDFEKLDK--LTQGLDIKWVRILKGH 279
[Close]
BLAST of Ribonuclease H1 vs. Ensembl Xenopus
Match: lrrc1 (leucine rich repeat containing 1 [Source:Xenbase;Acc:XB-GENE-943417])

HSP 1 Score: 119.398 bits (298), Expect = 2.210e-31
Identity = 67/142 (47.18%), Postives = 84/142 (59.15%), Query Frame = 1 
Query:  487 VYTDGSC-RKLKTGCIAGYGVYWGKNSPWNVGEKYVGVQTNNRAEIAAVISAVETASKNGIDKLRIITDSQFTINCMTKWIGKWKRNNWMTSGNKEAQCRDVLEKLDNLILSTKLDIIWEHVNGHSGNPGNEAADQLANLGA 909
            VYTDG C R  + G   G GVYWG     NV  K  G QTN RAEI A  +AV+ A  + I  L+I TDS+FTIN MT+W+ KWK N W T   ++   +   +KLD       +DI W +V GHSG+ GN+ ADQLA  G+
Sbjct:   63 VYTDGCCSRNGQYGANGGIGVYWGPGDSRNVSAKLEGRQTNQRAEIEAARTAVKQARDDNITSLQINTDSKFTINGMTQWVPKWKENGWKTVDGRDVINKQDFQKLDKAC--ENMDIRWNYVPGHSGSVGNDRADQLAKAGS 202
[Close]
BLAST of Ribonuclease H1 vs. Ensembl Xenopus
Match: lrrc1 (leucine rich repeat containing 1 [Source:Xenbase;Acc:XB-GENE-943417])

HSP 1 Score: 119.398 bits (298), Expect = 2.210e-31
Identity = 67/142 (47.18%), Postives = 84/142 (59.15%), Query Frame = 1 
Query:  487 VYTDGSC-RKLKTGCIAGYGVYWGKNSPWNVGEKYVGVQTNNRAEIAAVISAVETASKNGIDKLRIITDSQFTINCMTKWIGKWKRNNWMTSGNKEAQCRDVLEKLDNLILSTKLDIIWEHVNGHSGNPGNEAADQLANLGA 909
            VYTDG C R  + G   G GVYWG     NV  K  G QTN RAEI A  +AV+ A  + I  L+I TDS+FTIN MT+W+ KWK N W T   ++   +   +KLD       +DI W +V GHSG+ GN+ ADQLA  G+
Sbjct:   63 VYTDGCCSRNGQYGANGGIGVYWGPGDSRNVSAKLEGRQTNQRAEIEAARTAVKQARDDNITSLQINTDSKFTINGMTQWVPKWKENGWKTVDGRDVINKQDFQKLDKAC--ENMDIRWNYVPGHSGSVGNDRADQLAKAGS 202
[Close]
BLAST of Ribonuclease H1 vs. Ensembl Xenopus
Match: lrrc1 (leucine rich repeat containing 1 [Source:Xenbase;Acc:XB-GENE-943417])

HSP 1 Score: 118.242 bits (295), Expect = 5.736e-31
Identity = 66/142 (46.48%), Postives = 82/142 (57.75%), Query Frame = 1 
Query:  487 VYTDGSC-RKLKTGCIAGYGVYWGKNSPWNVGEKYVGVQTNNRAEIAAVISAVETASKNGIDKLRIITDSQFTINCMTKWIGKWKRNNWMTSGNKEAQCRDVLEKLDNLILSTKLDIIWEHVNGHSGNPGNEAADQLANLGA 909
            VYTDG C R  + G   G GVYWG     NV  K  G QTN RAEI A  +AVE A  + I  L I TDS+FTI  MT+W+ +WK N W T   ++   +   +KLD       +DI W +V GHSG+ GN+ ADQLA  G+
Sbjct:   52 VYTDGCCYRNGQYGANGGIGVYWGPEDSRNVSAKLKGRQTNQRAEIEAACTAVEQARDDNITSLNINTDSKFTIKGMTQWVPRWKENGWKTIDGRDVINKQDFQKLDKAC--ENMDIKWNYVPGHSGSVGNDRADQLAKAGS 191
[Close]
BLAST of Ribonuclease H1 vs. Ensembl Mouse
Match: Rnaseh1 (ribonuclease H1 [Source:MGI Symbol;Acc:MGI:1335073])

HSP 1 Score: 150.214 bits (378), Expect = 3.253e-42
Identity = 101/273 (37.00%), Postives = 130/273 (47.62%), Query Frame = 1 
Query:  103 FYVVINGREPGIYKTWLIGL*FWFLNNSFRPECQRQVDNFPNAKFKGFMTKEEADNYIDISKSGSGKNLFKRKIESDMNLFAPKRVKIDKPTTNPLEF-EFCIKQQKESYIKGIKVDLLRDPIEPNRVCVYTDGSCRK-LKTGCIAGYGVYWGKNSPWNVGEKYVGVQTNNRAEIAAVISAVETASKNGIDKLRIITDSQFTINCMTKWIGKWKRNNWMTSGNKEAQCRDVLEKLDNLILSTKLDIIWEHVNGHSGNPGNEAADQLANLGADQ 915
            FY V  GR  G++ +W               EC+ QVD FP A+FK F T++EA  ++  S S  G    +   E        KR +  +P     E  E   K  ++       V           V VYTDG C    +    AG GVYWG   P NVG +  G QTN RAEI A   A+  A    I KL + TDS FTIN +T W+  WK+N W TS  K+   ++   +LD   L+  +DI W H+ GHSG  GNE AD+LA  GA Q
Sbjct:   28 FYAVRRGRRTGVFLSW--------------SECKAQVDRFPAARFKKFATEDEAWAFVRSSSSPDGSKGQESAHEQKSQAKTSKRPR--EPLGEGEELPEPGPKHTRQDTEPSAVVSKDAFSYMGESVVVYTDGCCSSNGRKRARAGIGVYWGPGHPLNVGIRLPGRQTNQRAEIHAACKAIMQAKAQNISKLVLYTDSMFTINGITNWVQGWKKNGWRTSTGKDVINKEDFMELDE--LTQGMDIQWMHIPGHSGFVGNEEADRLAREGAKQ 282
[Close]
BLAST of Ribonuclease H1 vs. Ensembl Mouse
Match: Rnaseh1 (ribonuclease H1 [Source:MGI Symbol;Acc:MGI:1335073])

HSP 1 Score: 56.9954 bits (136), Expect = 4.481e-9
Identity = 48/162 (29.63%), Postives = 64/162 (39.51%), Query Frame = 1 
Query:  103 FYVVINGREPGIYKTWLIGL*FWFLNNSFRPECQRQVDNFPNAKFKGFMTKEEADNYIDISKSGSGKNLFKRKIESDMNLFAPKRVKIDKPTTNPLEF-EFCIKQQKESYIKGIKVDLLRDPIEPNRVCVYTDGSCRK-LKTGCIAGYGVYWGKNSPWNVGE 582
            FY V  GR  G++ +W               EC+ QVD FP A+FK F T++EA  ++  S S  G    +   E        KR +  +P     E  E   K  ++       V           V VYTDG C    +    AG GVYWG   P+  G 
Sbjct:   28 FYAVRRGRRTGVFLSWS--------------ECKAQVDRFPAARFKKFATEDEAWAFVRSSSSPDGSKGQESAHEQKSQAKTSKRPR--EPLGEGEELPEPGPKHTRQDTEPSAVVSKDAFSYMGESVVVYTDGCCSSNGRKRARAGIGVYWGPGHPFLQGH 173
[Close]
BLAST of Ribonuclease H1 vs. UniProt/SwissProt
Match: sp|O60930|RNH1_HUMAN (Ribonuclease H1 OS=Homo sapiens OX=9606 GN=RNASEH1 PE=1 SV=2)

HSP 1 Score: 157.918 bits (398), Expect = 3.286e-44
Identity = 106/282 (37.59%), Postives = 141/282 (50.00%), Query Frame = 1 
Query:  103 FYVVINGREPGIYKTWLIGL*FWFLNNSFRPECQRQVDNFPNAKFKGFMTKEEADNYIDISKSGSGKNLFKRKIESDMNLFAPKRVKIDKPTTNPLEFEFCIKQQKESYIKGIKVDLLRDPIEP----------NRVCVYTDGSCRK-LKTGCIAGYGVYWGKNSPWNVGEKYVGVQTNNRAEIAAVISAVETASKNGIDKLRIITDSQFTINCMTKWIGKWKRNNWMTSGNKEAQCRDVLEKLDNLILSTKLDIIWEHVNGHSGNPGNEAADQLANLGADQ 915
            FY V  GR+ G++ TW               EC+ QVD FP A+FK F T++EA  ++  S S       + +   +    A KR++       PL+ +    +  E Y K +K  +  +P  P          + V VYTDG C    +    AG GVYWG   P NVG +  G QTN RAEI A   A+E A    I+KL + TDS FTIN +T W+  WK+N W TS  KE   ++    L+   L+  +DI W HV GHSG  GNE AD+LA  GA Q
Sbjct:   28 FYAVRRGRKTGVFLTW--------------NECRAQVDRFPAARFKKFATEDEAWAFVRKSASPEVSEGHENQHGQESEAKASKRLR------EPLDGDG--HESAEPYAKHMKPSV--EPAPPVSRDTFSYMGDFVVVYTDGCCSSNGRRRPRAGIGVYWGPGHPLNVGIRLPGRQTNQRAEIHAACKAIEQAKTQNINKLVLYTDSMFTINGITNWVQGWKKNGWKTSAGKEVINKEDFVALER--LTQGMDIQWMHVPGHSGFIGNEEADRLAREGAKQ 283
[Close]
BLAST of Ribonuclease H1 vs. UniProt/SwissProt
Match: sp|O70338|RNH1_MOUSE (Ribonuclease H1 OS=Mus musculus OX=10090 GN=Rnaseh1 PE=2 SV=1)

HSP 1 Score: 150.599 bits (379), Expect = 1.943e-41
Identity = 101/273 (37.00%), Postives = 130/273 (47.62%), Query Frame = 1 
Query:  103 FYVVINGREPGIYKTWLIGL*FWFLNNSFRPECQRQVDNFPNAKFKGFMTKEEADNYIDISKSGSGKNLFKRKIESDMNLFAPKRVKIDKPTTNPLEF-EFCIKQQKESYIKGIKVDLLRDPIEPNRVCVYTDGSCRK-LKTGCIAGYGVYWGKNSPWNVGEKYVGVQTNNRAEIAAVISAVETASKNGIDKLRIITDSQFTINCMTKWIGKWKRNNWMTSGNKEAQCRDVLEKLDNLILSTKLDIIWEHVNGHSGNPGNEAADQLANLGADQ 915
            FY V  GR  G++ +W               EC+ QVD FP A+FK F T++EA  ++  S S  G    +   E        KR +  +P     E  E   K  ++       V           V VYTDG C    +    AG GVYWG   P NVG +  G QTN RAEI A   A+  A    I KL + TDS FTIN +T W+  WK+N W TS  K+   ++   +LD   L+  +DI W H+ GHSG  GNE AD+LA  GA Q
Sbjct:   28 FYAVRRGRRTGVFLSW--------------SECKAQVDRFPAARFKKFATEDEAWAFVRSSSSPDGSKGQESAHEQKSQAKTSKRPR--EPLGEGEELPEPGPKHTRQDTEPAAVVSKDTFSYMGESVIVYTDGCCSSNGRKRARAGIGVYWGPGHPLNVGIRLPGRQTNQRAEIHAACKAIMQAKAQNISKLVLYTDSMFTINGITNWVQGWKKNGWRTSTGKDVINKEDFMELDE--LTQGMDIQWMHIPGHSGFVGNEEADRLAREGAKQ 282
[Close]
BLAST of Ribonuclease H1 vs. UniProt/SwissProt
Match: sp|Q5BK46|RNH1_RAT (Ribonuclease H1 OS=Rattus norvegicus OX=10116 GN=Rnaseh1 PE=2 SV=1)

HSP 1 Score: 149.443 bits (376), Expect = 5.446e-41
Identity = 103/304 (33.88%), Postives = 139/304 (45.72%), Query Frame = 1 
Query:   64 FKFVVILLKMVKK-------FYVVINGREPGIYKTWLIGL*FWFLNNSFRPECQRQVDNFPNAKFKGFMTKEEADNYIDISKSGSGKNLFKRKIESDMNLFAPKRVKID------------KPTTNPLEFEFCIKQQKESYIKGIKVDLLRDPIEPNRVCVYTDGSCRK-LKTGCIAGYGVYWGKNSPWNVGEKYVGVQTNNRAEIAAVISAVETASKNGIDKLRIITDSQFTINCMTKWIGKWKRNNWMTSGNKEAQCRDVLEKLDNLILSTKLDIIWEHVNGHSGNPGNEAADQLANLGADQ 915
            F+ V + +  V++       FY V  GR  G++ +W               EC+ QVD FP A+FK F T++EA  ++  S S  G    +      + +   KR +              K T    E    + +   SY+                V VYTDG C    +    AG GVYWG   P NVG +  G QTN RAEI A   A+  A    I KL + TDS FTIN +T W+  WK+N W TS  K+   ++   +LD   L+  +DI W H+ GHSG  GNE AD+LA  GA Q
Sbjct:    8 FRTVTLAVVRVRRGVCGLGMFYAVRRGRRTGVFLSW--------------SECKAQVDRFPAARFKKFATEDEAWAFVRSSSSPDGSKGQESAHVQKLQVKTSKRPREPLGEEEEPPEPGAKHTRQDTEPAALVSKDAFSYMG-------------ESVVVYTDGCCSSNGRKRARAGIGVYWGPGHPLNVGIRLPGRQTNQRAEIHAACKAITQAKAQNISKLVLYTDSMFTINGITNWVQGWKKNGWRTSTGKDVINKEDFMELDE--LTQGMDIQWMHIPGHSGFVGNEEADRLAREGAKQ 282
[Close]
BLAST of Ribonuclease H1 vs. UniProt/SwissProt
Match: sp|Q9UST8|RNH1_SCHPO (Ribonuclease H OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=rnh1 PE=1 SV=1)

HSP 1 Score: 106.686 bits (265), Expect = 2.949e-25
Identity = 83/290 (28.62%), Postives = 130/290 (44.83%), Query Frame = 1 
Query:   97 KKFYVVINGREPGIYKTWLIGL*FWFLNNSFRPECQRQVDNFPNAKFKGFMTKEEADNY--IDISKSGSGKNLFKRKIESDMNLFAPK-------------RVKIDKPTTNPLEFEFCIKQQKESYIKGIKVDLLRDPIEPNRVCVYTDGSC-RKLKTGCIAGYGVYWGKNSPWNVGEKYVGV-QTNNRAEIAAVISAVETASKNGIDKLRIITDSQFTINCMTKWIGKWKRNNWMTSGNKEAQCRDVLEKLDNLILSTKLDIIWEHVNGHSGNPGNEAADQLANLGADQ 915
            + +Y V  GR  GIY TW               E   QV  +   ++K F + E A  +   + S+  S    ++R   S                     R KI +  +   + E       E  I              +R  VY DGS  R  K G +AG GV++G + P N+     G  QTNNRAE+ A+I A+E  S +    L I +DS ++I  +T W+ KWK+N++ TS ++  +  D++ +  +L+  +  ++  E+V GHS + GN+ AD LA  GA +
Sbjct:    6 RAYYAVARGRNTGIYSTW--------------DEASDQVKGYGGNRYKKFDSYEAAQEFCRTEGSRYSSSSGPYRRSTTSYGYSPYSSSSSNYSARHSDKYRKKISRSYSTEKDIEIFSNDTHEKSIAC-----------SDRQVVYADGSSLRNGKKGAVAGCGVFFGNDDPRNISVPLAGEEQTNNRAELQAIILALENTSGD----LTIRSDSNYSIKSLTTWLPKWKKNDFKTSNSQPVKNLDLINRASDLM--SDRNVSLEYVKGHSTDYGNQQADMLARRGASE 264
[Close]
BLAST of Ribonuclease H1 vs. UniProt/SwissProt
Match: sp|A5CX29|RNH_VESOH (Ribonuclease H OS=Vesicomyosocius okutanii subsp. Calyptogena okutanii (strain HA) OX=412965 GN=rnhA PE=3 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.408e-21
Identity = 60/150 (40.00%), Postives = 88/150 (58.67%), Query Frame = 1 
Query:  475 NRVCVYTDGSCRKLKTGCIAGYGVYWGKNSPWNVGEKYVGVQ---TNNRAEIAAVISAVETASKNGIDKLRIITDSQFTINCMTKWIGKWKRNNWMTSGNKEAQCRDVLEKLDNLILSTKLDIIWEHVNGHSGNPGNEAADQLANLGADQ 915
            NR+ +YTDG CR   TG I G+GV W K   ++  +K  G +   TNNR E+ A I A+E    N I  + + TDS++ I  + +W+  WK  NW TS  +  +  D+ ++LD  +L+ + D+IW  V  HS N GN+ AD+LANL  D+
Sbjct:    3 NRIVIYTDGGCR-CNTG-IGGWGV-WLKYGDYD--KKLKGNEKKTTNNRMELTATIKALEEIKSNQIG-IDLFTDSKYVITGINEWMKNWKVKNWETSKKRPVKNVDLWQRLD--VLNKQHDVIWYWVKSHSNNKGNDIADELANLAMDK 144
[Close]
BLAST of Ribonuclease H1 vs. TrEMBL
Match: A0A564YWP4 (Ribonuclease H1 OS=Hymenolepis diminuta OX=6216 GN=WMSIL1_LOCUS10510 PE=3 SV=1)

HSP 1 Score: 180.259 bits (456), Expect = 1.929e-50
Identity = 108/271 (39.85%), Postives = 147/271 (54.24%), Query Frame = 1 
Query:  103 FYVVINGREPGIYKTWLIGL*FWFLNNSFRPECQRQVDNFPNAKFKGFMTKEEADNYIDISKSGSGKNLFKRKIESDMNLFAPKRVKIDKPTTNPLEFEFCIKQQKESYIKGIK-VDLLRDPIEPNRVCVYTDGSCRKLKTGCIAGYGVYWGKNSPWNVGEKYVGVQTNNRAEIAAVISAVETASKNGIDKLRIITDSQFTINCMTKWIGKWKRNNWMTSGNKEAQCRDVLEKLDNLILSTKLDIIWEHVNGHSGNPGNEAADQLANLGAD 912
            FYVV  G+ PGIY TW               ECQ+QV  F  A F+ F T  EA+ +++     S   + KR I +D     P  +KI+K           ++    + +  +  V  + D  EPN+V VYTDGSC+  +    AG GV+WG+N PWNV E+  G QTNNRAEI A I A++ A+K GI  ++I+TDS+FTINC T W  KW +N W  +       +  +++L + + S  L + W H  GH G  GNE ADQLAN GAD
Sbjct:    3 FYVVKAGKTPGIYGTW--------------SECQKQVHGFTGAIFRKFSTMAEAEKFLEDDVPVS--FVRKRLIATDHTGEPP--LKINK-----------VRVVDSTAVDNLSPVAFMHDVHEPNKVLVYTDGSCKGQQNNRRAGIGVFWGENHPWNVAERLSGRQTNNRAEIEACIRALQQANKAGIMGVKIVTDSKFTINCATIWYFKWIKNGWKLANGAPVLLKADIQRLADELASPGLRVSWCHSPGHRGKWGNEQADQLANAGAD 244
[Close]
BLAST of Ribonuclease H1 vs. TrEMBL
Match: A0A4Z2DL37 (Ribonuclease H1 OS=Schistosoma japonicum OX=6182 GN=EWB00_011253 PE=3 SV=1)

HSP 1 Score: 176.792 bits (447), Expect = 2.344e-49
Identity = 92/246 (37.40%), Postives = 135/246 (54.88%), Query Frame = 1 
Query:  196 ECQRQVDNFPNAKFKGFMTKEEADNYIDISKSGSGKNLFKRKIESDMNLFAPKRVKIDKPTTNPLEFEFCIKQQKESYIKGIK----VDLLRDPIEPNRVCVYTDGSCRKLKTGCIAGYGVYWGKNSPWNVGEKYVGVQTNNRAEIAAVISAVETASKNGIDKLRIITDSQFTINCMTKWIGKWKRNNWMTSGNKEAQCRDVLEKLDNLILSTKLDIIWEHVNGHSGNPGNEAADQLANLGADQWL 921
            EC+R V  +  A++K F + +EA  +++               E +      KR+K+    ++           +E+ +        +    D   P RV VYTDGSC+       AGYGVYWG + PWNV E+  G QTNNRAEI AVI A++ A  +GI  L I+TDS+FT NC T+W  +W RN+W  +  ++A+ ++ +++L  LI  + ++I W HV GH G  GNE AD LAN+GAD+ L
Sbjct:   27 ECERHVKGYTGARYKKFASYDEAKKFVEN--------------EHETETIPSKRLKVANGQSSQANLLLTTSSDEENSVHSDHSPHMITFYHDATCPRRVMVYTDGSCKGALDDRHAGYGVYWGPSHPWNVSERLEGEQTNNRAEIEAVICAIKQAHASGITHLGIVTDSKFTQNCATEWGKRWARNSWKLANGEDAKLKEPVKRLLELIAESGIEITWHHVRGHKGVHGNEEADCLANIGADKPL 258
[Close]
BLAST of Ribonuclease H1 vs. TrEMBL
Match: A0A3P4M4I4 (Ribonuclease H1 OS=Gulo gulo OX=48420 GN=BN2614_LOCUS1 PE=3 SV=1)

HSP 1 Score: 172.94 bits (437), Expect = 1.764e-47
Identity = 107/272 (39.34%), Postives = 140/272 (51.47%), Query Frame = 1 
Query:  103 FYVVINGREPGIYKTWLIGL*FWFLNNSFRPECQRQVDNFPNAKFKGFMTKEEADNYIDISKSGSGKNLFKRKIESDMNLFAPKRVKIDKPTTNPLEFEFCIKQQKESYIKGIKVDLLRDPIEPNRVCVYTDGSCRK-LKTGCIAGYGVYWGKNSPWNVGEKYVGVQTNNRAEIAAVISAVETASKNGIDKLRIITDSQFTINCMTKWIGKWKRNNWMTSGNKEAQCRDVLEKLDNLILSTKLDIIWEHVNGHSGNPGNEAADQLANLGADQ 915
            FY V  GR+ G+++TW               EC+ QVD FP A+FK F T+EEA  ++    S  G    + K + +  + A KR++  +P+      E C KQ +++      VD          V VYTDG C    +    AG GVYWG   P N+G +  G QTN RAEI A   A+E A    I KL + TDS FTIN +T WI  WK+N W TS  KE   ++    L+   L+  +DI W HV GHSG  GNE AD+LA  GA Q
Sbjct:   29 FYAVRRGRKAGVFQTW--------------SECRAQVDRFPAARFKKFATEEEAWAFVRKPASPGGSEGTENKRDQESQVKANKRLR--EPSDGEGNTEPCAKQARQTAESVPSVDKDTFSYMGESVVVYTDGCCSSNGRKRARAGIGVYWGPGHPLNIGIRLPGRQTNQRAEIHAACKAIEQAKAQNIKKLVLYTDSMFTINGVTNWIQGWKKNGWKTSAGKEVTNKEDFAVLEQ--LTQGMDIQWMHVPGHSGFVGNEEADRLAREGAKQ 282
[Close]
BLAST of Ribonuclease H1 vs. TrEMBL
Match: F1N2X2 (Ribonuclease H1 OS=Bos taurus OX=9913 GN=RNASEH1 PE=3 SV=3)

HSP 1 Score: 172.17 bits (435), Expect = 3.233e-47
Identity = 110/304 (36.18%), Postives = 150/304 (49.34%), Query Frame = 1 
Query:   37 NRIILDYYNFKFVVILLKMVKKFYVVINGREPGIYKTWLIGL*FWFLNNSFRPECQRQVDNFPNAKFKGFMTKEEADNYIDISKSGSGKNLFKRKIESDMNLFAPKRVKIDKPTTNPLEFEFCIKQQKESYIKGIKVDLLRDPIEP----------NRVCVYTDGSCRK-LKTGCIAGYGVYWGKNSPWNVGEKYVGVQTNNRAEIAAVISAVETASKNGIDKLRIITDSQFTINCMTKWIGKWKRNNWMTSGNKEAQCRDVLEKLDNLILSTKLDIIWEHVNGHSGNPGNEAADQLANLGADQ 915
             R++   +      +  + +  FY V  GR+ G++ TW               EC+ QVD FP A+FK F T+EEA  ++  S+S S     K K   +  + A KR++  +P     + E C K  ++S           +P+ P          + V VYTDG C    +    AG GVYWG   P NVG +  G QTN RAEI A   A+E A    I KL + TDS FTIN +T W+  WK+N W TS  KE   ++   +L+   L+  +DI W HV GHSG  GNE AD+LA  GA Q
Sbjct:    2 TRLLGVVHRVALAFVGCRSLAMFYAVRRGRKAGVFLTW--------------DECRAQVDRFPAARFKKFATEEEAWAFVRRSESPSDSEGQKTKHVEEPQVKASKRLR--EPLDEDEDAEACAKHVRQSA----------EPLPPVSKDTFSYMGDSVVVYTDGCCSSNGRKRARAGIGVYWGPGHPLNVGIRLPGRQTNQRAEIHAACKAIEQAKAQDITKLVLYTDSMFTINGITTWVKGWKQNGWRTSTGKEVTNKEDFAELER--LARGMDIQWMHVPGHSGFRGNEEADRLAREGAKQ 277
[Close]
BLAST of Ribonuclease H1 vs. TrEMBL
Match: A0A4W2H8H3 (Ribonuclease H1 OS=Bos indicus x Bos taurus OX=30522 GN=RNASEH1 PE=3 SV=1)

HSP 1 Score: 172.17 bits (435), Expect = 3.233e-47
Identity = 110/304 (36.18%), Postives = 150/304 (49.34%), Query Frame = 1 
Query:   37 NRIILDYYNFKFVVILLKMVKKFYVVINGREPGIYKTWLIGL*FWFLNNSFRPECQRQVDNFPNAKFKGFMTKEEADNYIDISKSGSGKNLFKRKIESDMNLFAPKRVKIDKPTTNPLEFEFCIKQQKESYIKGIKVDLLRDPIEP----------NRVCVYTDGSCRK-LKTGCIAGYGVYWGKNSPWNVGEKYVGVQTNNRAEIAAVISAVETASKNGIDKLRIITDSQFTINCMTKWIGKWKRNNWMTSGNKEAQCRDVLEKLDNLILSTKLDIIWEHVNGHSGNPGNEAADQLANLGADQ 915
             R++   +      +  + +  FY V  GR+ G++ TW               EC+ QVD FP A+FK F T+EEA  ++  S+S S     K K   +  + A KR++  +P     + E C K  ++S           +P+ P          + V VYTDG C    +    AG GVYWG   P NVG +  G QTN RAEI A   A+E A    I KL + TDS FTIN +T W+  WK+N W TS  KE   ++   +L+   L+  +DI W HV GHSG  GNE AD+LA  GA Q
Sbjct:    2 TRLLGVVHRVALAFVGCRSLAMFYAVRRGRKAGVFLTW--------------DECRAQVDRFPAARFKKFATEEEAWAFVRRSESPSDSEGQKTKHVEEPQVKASKRLR--EPLDEDEDAEACAKHVRQSA----------EPLPPVSKDTFSYMGDSVVVYTDGCCSSNGRKRARAGIGVYWGPGHPLNVGIRLPGRQTNQRAEIHAACKAIEQAKAQDITKLVLYTDSMFTINGITTWVKGWKQNGWRTSTGKEVTNKEDFAELER--LARGMDIQWMHVPGHSGFRGNEEADRLAREGAKQ 277
[Close]
BLAST of Ribonuclease H1 vs. Ensembl Cavefish
Match: rnaseh1 (ribonuclease H1 [Source:ZFIN;Acc:ZDB-GENE-040718-407])

HSP 1 Score: 145.206 bits (365), Expect = 1.074e-40
Identity = 91/286 (31.82%), Postives = 137/286 (47.90%), Query Frame = 1 
Query:  103 FYVVINGREPGIYKTWLIGL*FWFLNNSFRPECQRQVDNFPNAKFKGFMTKEEA-DNYIDISKSGSGKNLFKRKIESDMNLFAPKRVKIDKPTTNPLEFEFCIKQQKESYIKGIKVDLLRDPIEP---------------NRVCVYTDGSC-RKLKTGCIAGYGVYWGKNSPWNVGEKYVGVQTNNRAEIAAVISAVETASKNGIDKLRIITDSQFTINCMTKWIGKWKRNNWMTSGNKEAQCRDVLEKLDNLILSTKLDIIWEHVNGHSGNPGNEAADQLANLGA 909
            FY V  G  PG+Y TW               EC+ QV+ FP A++K F ++E+A    + +S   +  +  +++  +  +L     V   +P  + ++ +  +K+ K+           ++P +P               + V VYTDG C R  KTG  AG GVYWG + P NV E+  G QTN RAE+ A   A+E A      K+ + TDS+FTIN +T W+ KWK N W                   L    +L+++W H+ GH+G  GNE AD+L+  GA
Sbjct:    8 FYAVRKGHNPGVYHTW--------------DECKFQVEKFPRAQYKKFASEEDAWLTTVHLSAQAANSSRLEQRPSNTSHLSTSVPVVAKRPIEDDIDDQSRLKRFKQ----------FKEPAQPKTSSSASTNGFTYMGDAVVVYTDGCCSRNGKTGARAGIGVYWGPDHPLNVAERLPGRQTNQRAELQAACKALEQARDMDFKKIVLYTDSKFTINGITSWVKKWKTNGW------------------KLNTGGELEVVWLHIAGHAGFKGNEEADRLSREGA 251
[Close]
BLAST of Ribonuclease H1 vs. Ensembl Cavefish
Match: rnaseh1 (ribonuclease H1 [Source:ZFIN;Acc:ZDB-GENE-040718-407])

HSP 1 Score: 130.568 bits (327), Expect = 4.114e-35
Identity = 89/277 (32.13%), Postives = 131/277 (47.29%), Query Frame = 1 
Query:  103 FYVVINGREPGIYKTWLIGL*FWFLNNSFRPECQRQVDNFPNAKFKGFMTKEEADNYIDISKSGSGKNLFKRKIESDMNLFAPKRVKIDKPTTNPLEFEFCIKQQKESYIKGIKVDLLRDPIEP-------NRVCVYTDGSC-RKLKTGCIAGYGVYWGKNSPWNVGEKYVGVQTNNRAEIAAVISAVETASKNGIDKLRIITDSQFTINCMTKWIGKWKRNNWMTSGNKEAQCRDVLEKLDNLILSTKLDIIWEHVNGHSGNPGNEAADQLANLGA 909
            FY V  G  PG+Y TW               EC+ QV+ FP A++K F ++E+A  ++     G+          +   +     V   +P  + ++ +  +K+ K+           ++P +P       + V VYTDG C R  KTG  AG GVYWG + P NV E+  G QTN RAE+ A   A+E A      K+ + TDS+FTIN +T W+ KWK N W                     L+T  +++  H+ GH+G  GNE AD+L+  GA
Sbjct:   29 FYAVRKGHNPGVYHTW--------------DECKFQVEKFPRAQYKKFASEEDAWAFV-----GNKAPELPSTTTTATEVSTSVPVVAKRPIEDDIDDQSRLKRFKQ----------FKEPAQPKTSSSARDAVVVYTDGCCSRNGKTGARAGIGVYWGPDHPLNVAERLPGRQTNQRAELQAACKALEQARDMDFKKIVLYTDSKFTINGITSWVKKWKTNGWK--------------------LNTGGEVL--HIAGHAGFKGNEEADRLSREGA 254
[Close]
BLAST of Ribonuclease H1 vs. Ensembl Sea Lamprey
Match: rnaseh1 (ribonuclease H1 [Source:ZFIN;Acc:ZDB-GENE-040718-407])

HSP 1 Score: 157.532 bits (397), Expect = 4.723e-46
Identity = 97/279 (34.77%), Postives = 133/279 (47.67%), Query Frame = 1 
Query:   97 KKFYVVINGREPGIYKTWLIGL*FWFLNNSFRPECQRQVDNFPNAKFKGFMTKEEADNYIDISKSGSGKNL-----FKRKIESDMNLFAPKRVKIDKPTTNPLEFEFCIKQQKESYIKGIKVDLLRDPIEPNRVCVYTDGSC-RKLKTGCIAGYGVYWGKNSPWNVGEKYVGVQTNNRAEIAAVISAVETASKNGIDKLRIITDSQFTINCMTKWIGKWKRNNWMTSGNKEAQCRDVLEKLDNLILSTKLDIIWEHVNGHSGNPGNEAADQLANLGADQ 915
            + +Y V  GR PG+Y TW               EC+ QV+ FP A F+ F+ +  A  Y+   + G G         +R+ E +           ++P            Q + +Y+ G+ V             V+TDG C    +    AG GV+WG N+P N+ E+ VG QTN RAEI A   AV+ AS+ G+ KL + TDS F +N M  W+  WKR+ W          R     LD+L   + +DI W HV GHSGN GNE ADQLA  GAD+
Sbjct:   20 RGYYAVRIGRSPGLYATW--------------DECRTQVEGFPGADFRKFLCEGTAHAYLAQGRGGDGAATERPPQLRRRWEDE-----------EQPADRG-------DQPRFTYVGGVPV-------------VFTDGCCISNGRQAARAGLGVFWGTNNPLNLSERLVGRQTNQRAEIMAACRAVQQASEQGLRKLIVCTDSMFIVNGMGSWVHTWKRSGWRQGSGSLVVNRADFALLDDLC--SDMDITWVHVQGHSGNLGNEHADQLAKEGADK 251
[Close]
BLAST of Ribonuclease H1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000003109.1 (pep scaffold:Pmarinus_7.0:GL476998:45325:46367:1 gene:ENSPMAG00000002846.1 transcript:ENSPMAT00000003109.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 140.198 bits (352), Expect = 4.650e-39
Identity = 106/306 (34.64%), Postives = 140/306 (45.75%), Query Frame = 1 
Query:  103 FYVVINGREPGIYKTWLIGL*FWFLNNSFRPECQRQVDNFPNAKFKGFMTKEEADNYI-------------DISKSGSGKNLF--------------------KRK--IESDMNLFAPKRVKIDKPTTNPLEFEFCIKQQKESYIKGIKVDLLRD-PIEPNRVCVYTDGSCR-KLKTGCIAGYGVYWGKNSPWNVGEKYVGVQTNNRAEIAAVISAVETASKNGIDKLRIITDSQFTINCMTKWIGKWKRNNWMTSGNKEAQCRDVLEKLDNLILSTKLDIIWEHVNGHSGNPGNEAADQLANLGA 909
            FY V  GR+PG+Y TW               EC  QV  F    FK F T+EEA N++                  GSG N                      KR+  + SD +    KR+K       P        + K         + L D P+      VYTDG C    K G  AG GVYWG +SP N+ EK  G QTN RAEI A   A+E A    ++KL + TDS+FTIN +TKW+  WK+  W+ S       ++  E+LD   L+  +++ W +V GHSG+ GNEAAD+LA  G+
Sbjct:    8 FYAVRRGRQPGVYLTW--------------EECLAQVTEFRGPAFKKFPTEEEAWNFVGPDEGGSKKPGGSSAGAKGSGPNKASTSRAAAAAATSTAAAKSGGKRRADLSSDEDEEPAKRMKATPAQPPPTTTTVATTELKPQ-------EFLGDVPV------VYTDGCCTMNGKHGACAGIGVYWGDDSPLNLSEKLQGRQTNQRAEIIAACRALEQAHSMKLEKLVLYTDSKFTINGITKWVKNWKKKGWVLSTGGPVVNKEDFERLDK--LNEGIEVSWMYVPGHSGHKGNEAADKLAKCGS 284
[Close]
BLAST of Ribonuclease H1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000562.1 (pep scaffold:Pmarinus_7.0:GL477719:55472:62226:1 gene:ENSPMAG00000000507.1 transcript:ENSPMAT00000000562.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 132.88 bits (333), Expect = 6.660e-38
Identity = 71/144 (49.31%), Postives = 90/144 (62.50%), Query Frame = 1 
Query:  481 VCVYTDGSC-RKLKTGCIAGYGVYWGKNSPWNVGEKYVGVQTNNRAEIAAVISAVETASKNGIDKLRIITDSQFTINCMTKWIGKWKRNNWMTSGNKEAQCRDVLEKLDNLILSTKLDIIWEHVNGHSGNPGNEAADQLANLGA 909
            V VYTDG C R  + G   G GVYWG  +P NV EK  G  TN RAEI A   A+E A +  +DKL I TDS+FTI+  TKW+  WK+N W T   +  + +   +KLD   LS  +D+ W+HV GHSG+ GNEAAD+LA  G+
Sbjct:    3 VSVYTDGCCTRNGQPGASGGVGVYWGDGNPMNVSEKLGGRPTNQRAEIEAASRALEQAREMNLDKLTIHTDSKFTIDGATKWVNTWKQNGWKTVTGEPVKNKAEFQKLDR--LSQGIDVTWKHVPGHSGHKGNEAADRLAREGS 144
[Close]
BLAST of Ribonuclease H1 vs. Ensembl Yeast
Match: RNH1 (Ribonuclease H1; able to bind double-stranded RNAs and RNA-DNA hybrids; associates with RNAse polymerase I. [Source:SGD;Acc:S000004847])

HSP 1 Score: 53.9138 bits (128), Expect = 1.373e-8
Identity = 56/184 (30.43%), Postives = 79/184 (42.93%), Query Frame = 1 
Query:  442 KVDLLRDPIEPNRVCVYTDGSCRKLKTGCI-AGYGVYWGKNSPWNVGEKYV-GVQTNNRAEIAAVISAVE------TASKNGIDKLRIITDSQFTINCMTKWIGKWKRNNWMTSGNKEAQC---RDVLEKLDNLILSTK---------------LDIIWEHVNGHSGNPGNEAADQLANLGADQ 915
            K  L  + +    + VY DGS     T    AGYG Y+      N+ E  + G QTNNRAEI AV  A++      T  K  ++  +I TDS++    +         + +MT  NK+ +     D++  L    +  K                 I W  V GH G+PGNE AD LA  GA +
Sbjct:  175 KYKLSSNTMYNKSMNVYCDGSSFGNGTSSSRAGYGAYFEGAPEENISEPLLSGAQTNNRAEIEAVSEALKKIWEKLTNEKEKVN-YQIKTDSEYVTKLLN--------DRYMTYDNKKLEGLPNSDLIVPLVQRFVKVKKYYELNKECFKNNGKFQIEW--VKGHDGDPGNEMADFLAKKGASR 347
[Close]
BLAST of Ribonuclease H1 vs. Ensembl Nematostella
Match: EDO39323 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SAK2])

HSP 1 Score: 153.295 bits (386), Expect = 5.596e-44
Identity = 99/278 (35.61%), Postives = 138/278 (49.64%), Query Frame = 1 
Query:  103 FYVVINGREPGIYKTWLIGL*FWFLNNSFRPECQRQVDNFPNAKFKGFMTKEEADNYID----ISKSGSGKNLFKRKIESDMNLFAPKRVKIDKP-TTNPLEFEFCIKQQKESYIKGIKVDL-LRDPIEPNRVCVYTDGSC-RKLKTGCIAGYGVYWGKNSPWNVGEKYVGVQTNNRAEIAAVISAVETASKNGIDKLRIITDSQFTINCMTKWIGKWKRNNWMTSGNKEAQCRDVLEKLDNLILSTKLDIIWEHVNGHSGNPGNEAADQLANLGADQ 915
            FY V  GR+ G++ TW               EC+  V  +  AKFK F + EEA ++++     S   +                 P   K     TT+        + +   Y+  +   +      E +R  VYTDG C R   +G  AG GVYWG N   N+ E+  G QTN RAEI A   A+E+A + G  K+ I TDS++TIN MT WI  WK+N W T+ NK+ +  +  +KLD L   +++D+ W HV GH+G  GNE ADQLA  GAD+
Sbjct:    8 FYAVRVGRQTGVFDTW--------------EECKSYVSGYAGAKFKKFGSNEEALDFVNGGSSSSHFATSSLSSSHGSSGSSGYVQPCPTKRHHSETTSSFGHSGTKRFKSTQYLDTVPASVGCTTSSERDRPVVYTDGCCTRNGFSGAKAGVGVYWGPNHKRNISERLPGRQTNQRAEIVAATMALESAKEMGFTKVEIRTDSKYTINGMTSWIRSWKKNGWKTADNKDVKNLEDFQKLDKLC--SEIDVHWTHVAGHAGIEGNEIADQLAKGGADK 269
[Close]
BLAST of Ribonuclease H1 vs. Ensembl Medaka
Match: rnaseh1 (ribonuclease H1 [Source:NCBI gene;Acc:101171879])

HSP 1 Score: 147.517 bits (371), Expect = 2.436e-41
Identity = 100/292 (34.25%), Postives = 143/292 (48.97%), Query Frame = 1 
Query:   97 KKFYVVINGREPGIYKTWLIGL*FWFLNNSFRPECQRQVDNFPNAKFKGFMTKEEADNYIDISKSGSGKNLFKRKIESDMNLFAPKRVKIDKPTTNPLEF--------------EFCIKQQKESYIKGIKVDLLRDPIEPNRVCVYTDGSCR-KLKTGCIAGYGVYWGKNSPWNVGEKYVGVQTNNRAEIAAVISAVETASKNGIDKLRIITDSQFTINCMTKWIGKWKRNNWMTSGNKEAQCRDVLEKLDNLILSTKLDIIWEHVNGHSGNPGNEAADQLANLGADQWLNE 927
            K FY V  G +PG+Y +W               EC+ QV+ FP A FK F ++ +A  ++   +  +     ++  E+++ L  PKR         PL +              E   K+ +ES     +          + V VYTDG C    K G  AG GVYWG N P NV E+  G QTN RAEI A   A++ A ++GI KL + TDS+FTIN +T W+  WK N W          ++   KLD   L+++L+++W H+ GHSG  GNE AD+L+  GA + L  
Sbjct:    5 KFFYAVRKGFKPGVYSSW--------------DECRSQVEKFPAASFKKFASERDAWAFVRGVELSAPP---RQSAEAEVPLL-PKR------GPEPLHYIPLGKRSRSSEEEEELHAKRPRESGAPSSEGTSDGFTYMGDAVVVYTDGCCSCNGKAGARAGIGVYWGHNHPLNVAERLEGRQTNQRAEIQAACKALQQAKEHGIQKLVLYTDSKFTINGVTSWVKNWKLNGWRLKSGGLVTNKEDFVKLDE--LNSELEVVWLHIPGHSGYTGNEQADRLSREGAGKPLQH 270
[Close]
BLAST of Ribonuclease H1 vs. Planmine SMEST
Match: SMESG000002048.1 (SMESG000002048.1)

HSP 1 Score: 542.347 bits (1396), Expect = 0.000e+0
Identity = 267/282 (94.68%), Postives = 267/282 (94.68%), Query Frame = 1 
Query:   91 MVKKFYVVINGREPGIYKTWLIGL*FWFLNNSFRPECQRQVDNFPNAKFKGFMTKEEADNYIDISKSGSGKNLFKRKIESDMNLFAPKRVKIDKPTTNPLEFEFCIKQQKESYIKGIKVDLLRDPIEPNRVCVYTDGSCRKLKTGCIAGYGVYWGKNSPWNVGEKYVGVQTNNRAEIAAVISAVETASKNGIDKLRIITDSQFTINCMTKWIGKWKRNNWMTSGNKEAQCRDVLEKLDNLILSTKLDIIWEHVNGHSGNPGNEAADQLANLGADQWLNEIIH 936
            MVKKFYVVINGREPGIYKTW              PECQRQVDNFPNAKFKGFMTKEEADNYIDISKS SGKNLFKRKIESDMNLFAPKRVKIDKPTTNPLEFEFCIKQQKESYIKGIKVDLLRDPIEPNRVCVYTDGSCRKLKTGCIAGYGVYWGKNSPWNVGEKYVGVQTNNRAEIAAVISAVETASKNGIDKLRIITDSQFTINCMTKWIGKWKRNNWMTSGNKEAQCRDVLEKLDNLILSTKLDIIWEHVNGHSGNPGNEAADQLANLGADQWLNEIIH
Sbjct:    1 MVKKFYVVINGREPGIYKTW--------------PECQRQVDNFPNAKFKGFMTKEEADNYIDISKSDSGKNLFKRKIESDMNLFAPKRVKIDKPTTNPLEFEFCIKQQKESYIKGIKVDLLRDPIEPNRVCVYTDGSCRKLKTGCIAGYGVYWGKNSPWNVGEKYVGVQTNNRAEIAAVISAVETASKNGIDKLRIITDSQFTINCMTKWIGKWKRNNWMTSGNKEAQCRDVLEKLDNLILSTKLDIIWEHVNGHSGNPGNEAADQLANLGADQWLNEIIH 268
[Close]
BLAST of Ribonuclease H1 vs. Planmine SMEST
Match: SMESG000002048.1 (SMESG000002048.1)

HSP 1 Score: 366.311 bits (939), Expect = 7.667e-128
Identity = 177/177 (100.00%), Postives = 177/177 (100.00%), Query Frame = 1 
Query:  406 IKQQKESYIKGIKVDLLRDPIEPNRVCVYTDGSCRKLKTGCIAGYGVYWGKNSPWNVGEKYVGVQTNNRAEIAAVISAVETASKNGIDKLRIITDSQFTINCMTKWIGKWKRNNWMTSGNKEAQCRDVLEKLDNLILSTKLDIIWEHVNGHSGNPGNEAADQLANLGADQWLNEIIH 936
            IKQQKESYIKGIKVDLLRDPIEPNRVCVYTDGSCRKLKTGCIAGYGVYWGKNSPWNVGEKYVGVQTNNRAEIAAVISAVETASKNGIDKLRIITDSQFTINCMTKWIGKWKRNNWMTSGNKEAQCRDVLEKLDNLILSTKLDIIWEHVNGHSGNPGNEAADQLANLGADQWLNEIIH
Sbjct:   24 IKQQKESYIKGIKVDLLRDPIEPNRVCVYTDGSCRKLKTGCIAGYGVYWGKNSPWNVGEKYVGVQTNNRAEIAAVISAVETASKNGIDKLRIITDSQFTINCMTKWIGKWKRNNWMTSGNKEAQCRDVLEKLDNLILSTKLDIIWEHVNGHSGNPGNEAADQLANLGADQWLNEIIH 200

HSP 2 Score: 51.6026 bits (122), Expect = 1.489e-7
Identity = 23/24 (95.83%), Postives = 24/24 (100.00%), Query Frame = 1 
Query:   91 MVKKFYVVINGREPGIYKTWLIGL 162
            MVKKFYVVINGREPGIYKTWLIG+
Sbjct:    1 MVKKFYVVINGREPGIYKTWLIGI 24
The following BLAST results are available for this feature:
BLAST of Ribonuclease H1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 3
Match NameE-valueIdentityDescription
RNASEH16.844e-4537.59ribonuclease H1 [Source:HGNC Symbol;Acc:HGNC:18466... [more]
AC108488.26.844e-4537.59novel protein[more]
AC108488.26.844e-4537.59novel protein[more]
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BLAST of Ribonuclease H1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 4
Match NameE-valueIdentityDescription
rnh-1.04.228e-3934.88Ribonuclease H1 [Source:UniProtKB/TrEMBL;Acc:Q86M... [more]
rnh-1.37.133e-1534.88RNase H [Source:UniProtKB/TrEMBL;Acc:Q9XVE6][more]
rnh-1.37.133e-1534.88RNase H [Source:UniProtKB/TrEMBL;Acc:Q9XVE6][more]
rnh-1.19.693e-828.57RNase H [Source:UniProtKB/TrEMBL;Acc:Q09633][more]
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BLAST of Ribonuclease H1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
rnh12.016e-3632.59gene:FBgn0023171 transcript:FBtr0088884[more]
rnh11.708e-2843.75gene:FBgn0023171 transcript:FBtr0088883[more]
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BLAST of Ribonuclease H1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
rnaseh13.014e-4234.15ribonuclease H1 [Source:ZFIN;Acc:ZDB-GENE-040718-4... [more]
rnaseh13.014e-4234.15ribonuclease H1 [Source:ZFIN;Acc:ZDB-GENE-040718-4... [more]
rnaseh12.701e-3132.67ribonuclease H1 [Source:ZFIN;Acc:ZDB-GENE-040718-4... [more]
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BLAST of Ribonuclease H1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
rnaseh19.582e-4837.25ribonuclease H1 [Source:NCBI gene;Acc:100124876][more]
rnaseh17.083e-3835.71ribonuclease H1 [Source:NCBI gene;Acc:100124876][more]
lrrc12.210e-3147.18leucine rich repeat containing 1 [Source:Xenbase;A... [more]
lrrc12.210e-3147.18leucine rich repeat containing 1 [Source:Xenbase;A... [more]
lrrc15.736e-3146.48leucine rich repeat containing 1 [Source:Xenbase;A... [more]
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BLAST of Ribonuclease H1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Rnaseh13.253e-4237.00ribonuclease H1 [Source:MGI Symbol;Acc:MGI:1335073... [more]
Rnaseh14.481e-929.63ribonuclease H1 [Source:MGI Symbol;Acc:MGI:1335073... [more]
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BLAST of Ribonuclease H1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|O60930|RNH1_HUMAN3.286e-4437.59Ribonuclease H1 OS=Homo sapiens OX=9606 GN=RNASEH1... [more]
sp|O70338|RNH1_MOUSE1.943e-4137.00Ribonuclease H1 OS=Mus musculus OX=10090 GN=Rnaseh... [more]
sp|Q5BK46|RNH1_RAT5.446e-4133.88Ribonuclease H1 OS=Rattus norvegicus OX=10116 GN=R... [more]
sp|Q9UST8|RNH1_SCHPO2.949e-2528.62Ribonuclease H OS=Schizosaccharomyces pombe (strai... [more]
sp|A5CX29|RNH_VESOH1.408e-2140.00Ribonuclease H OS=Vesicomyosocius okutanii subsp. ... [more]
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BLAST of Ribonuclease H1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A564YWP41.929e-5039.85Ribonuclease H1 OS=Hymenolepis diminuta OX=6216 GN... [more]
A0A4Z2DL372.344e-4937.40Ribonuclease H1 OS=Schistosoma japonicum OX=6182 G... [more]
A0A3P4M4I41.764e-4739.34Ribonuclease H1 OS=Gulo gulo OX=48420 GN=BN2614_LO... [more]
F1N2X23.233e-4736.18Ribonuclease H1 OS=Bos taurus OX=9913 GN=RNASEH1 P... [more]
A0A4W2H8H33.233e-4736.18Ribonuclease H1 OS=Bos indicus x Bos taurus OX=305... [more]
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BLAST of Ribonuclease H1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
rnaseh11.074e-4031.82ribonuclease H1 [Source:ZFIN;Acc:ZDB-GENE-040718-4... [more]
rnaseh14.114e-3532.13ribonuclease H1 [Source:ZFIN;Acc:ZDB-GENE-040718-4... [more]
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BLAST of Ribonuclease H1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 3
Match NameE-valueIdentityDescription
rnaseh14.723e-4634.77ribonuclease H1 [Source:ZFIN;Acc:ZDB-GENE-040718-4... [more]
ENSPMAT00000003109.14.650e-3934.64pep scaffold:Pmarinus_7.0:GL476998:45325:46367:1 g... [more]
ENSPMAT00000000562.16.660e-3849.31pep scaffold:Pmarinus_7.0:GL477719:55472:62226:1 g... [more]
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BLAST of Ribonuclease H1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
RNH11.373e-830.43Ribonuclease H1; able to bind double-stranded RNAs... [more]
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BLAST of Ribonuclease H1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO393235.596e-4435.61Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Ribonuclease H1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rnaseh12.436e-4134.25ribonuclease H1 [Source:NCBI gene;Acc:101171879][more]
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BLAST of Ribonuclease H1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000002048.10.000e+094.68SMESG000002048.1[more]
SMESG000002048.17.667e-128100.00SMESG000002048.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30020562 ID=SMED30020562|Name=Ribonuclease H1|organism=Schmidtea mediterranea sexual|type=transcript|length=1159bp
GAAATATTTAGATTCGTTAAAAGAAATTGTTTTTATAATCGAATTATTTT
AGATTATTATAATTTTAAATTTGTCGTAATTCTACTTAAAATGGTCAAGA
AATTTTACGTTGTAATAAACGGTCGAGAACCAGGAATATATAAAACATGG
TTAATAGGTTTGTGATTTTGGTTTTTAAACAATTCATTTAGGCCTGAATG
TCAACGACAAGTTGATAATTTCCCAAATGCTAAGTTTAAGGGGTTTATGA
CTAAAGAAGAGGCAGATAATTATATTGATATTTCTAAAAGTGGCTCGGGA
AAAAATTTATTTAAAAGAAAAATTGAATCAGACATGAACTTATTTGCTCC
TAAGCGCGTGAAAATAGATAAACCTACAACTAACCCTTTAGAATTTGAAT
TTTGTATTAAACAACAGAAAGAATCGTATATAAAAGGCATCAAAGTGGAT
TTATTGCGTGATCCGATTGAACCGAATAGAGTTTGTGTTTACACCGACGG
TTCATGTAGGAAACTGAAAACCGGCTGCATTGCAGGTTATGGAGTTTATT
GGGGCAAAAATAGTCCGTGGAATGTCGGTGAGAAATATGTTGGAGTTCAG
ACAAATAATCGGGCAGAAATAGCTGCTGTCATCAGTGCTGTGGAAACGGC
GAGCAAAAACGGCATCGATAAATTGCGGATTATCACAGACAGCCAATTCA
CGATCAACTGCATGACGAAATGGATCGGCAAATGGAAGCGAAACAATTGG
ATGACATCGGGAAACAAGGAAGCTCAATGCAGAGATGTTCTGGAGAAATT
GGATAACCTGATTCTCAGCACAAAGCTGGACATCATTTGGGAGCACGTGA
ACGGTCACTCCGGTAACCCGGGTAACGAGGCGGCAGATCAGCTGGCAAAT
CTCGGTGCAGATCAATGGCTCAATGAGATTATCCATTGATATTTCAATTA
ATAAATGCAATGAATTGTTTTGTGAATTCAACCTTTTTTTGCTAACTGTT
GCTATGGTGATAGCTGACCTAATTTAATGCAGTAAAATCTCGATATAACG
GACTAATCGGCATCCGGTGGTAAATCCTCTGGGCCTGAATTCACATTATT
ATCTCTATTTAACTGTCGTTATGTTTGTCTCGGAACTTGAATATCTATAT
TGAGTAAAT
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protein sequence of SMED30020562-orf-1

>SMED30020562-orf-1 ID=SMED30020562-orf-1|Name=SMED30020562-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=231bp
MTKEEADNYIDISKSGSGKNLFKRKIESDMNLFAPKRVKIDKPTTNPLEF
EFCIKQQKESYIKGIKVDLLRDPIEPNRVCVYTDGSCRKLKTGCIAGYGV
YWGKNSPWNVGEKYVGVQTNNRAEIAAVISAVETASKNGIDKLRIITDSQ
FTINCMTKWIGKWKRNNWMTSGNKEAQCRDVLEKLDNLILSTKLDIIWEH
VNGHSGNPGNEAADQLANLGADQWLNEIIH*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000429neoblast
PLANA:0002109X1 cell
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR036397RNaseH_sf
IPR012337RNaseH-like_sf
IPR002156RNaseH_domain
IPR017067RNase_H1_euk
IPR011320RNase_H1_N
IPR009027Ribosomal_L9/RNase_H1_N
Vocabulary: molecular function
TermDefinition
GO:0003676nucleic acid binding
GO:0004523RNA-DNA hybrid ribonuclease activity
GO:0000287magnesium ion binding
GO:0004518nuclease activity
GO:0004519endonuclease activity
GO:0016787hydrolase activity
GO:0046872metal ion binding
Vocabulary: biological process
TermDefinition
GO:0090502RNA phosphodiester bond hydrolysis, endonucleolytic
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036397Ribonuclease H superfamilyGENE3DG3DSA:3.30.420.10coord: 75..224
e-value: 1.7E-49
score: 169.5
IPR002156Ribonuclease H domainPFAMPF00075RNase_Hcoord: 77..221
e-value: 1.8E-35
score: 122.2
IPR002156Ribonuclease H domainPROSITEPS50879RNASE_Hcoord: 75..222
score: 24.705
IPR017067Ribonuclease H1, eukaryotePIRSFPIRSF036852RNase_H1_eukcoord: 1..226
e-value: 5.2E-50
score: 168.4
NoneNo IPR availablePANTHERPTHR10642:SF23RIBONUCLEASE H1coord: 40..222
NoneNo IPR availablePANTHERPTHR10642RIBONUCLEASE H1coord: 40..222
NoneNo IPR availableCDDcd09280RNase_HI_eukaryote_likecoord: 81..222
e-value: 2.78063E-67
score: 202.026
IPR012337Ribonuclease H-like superfamilySUPERFAMILYSSF53098Ribonuclease H-likecoord: 77..223