Ss-alanyl conjugating enzyme
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30020538 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 4
Homology
BLAST of Ss-alanyl conjugating enzyme vs. Ensembl Human
Match: AASDH (aminoadipate-semialdehyde dehydrogenase [Source:HGNC Symbol;Acc:HGNC:23993]) HSP 1 Score: 69.707 bits (169), Expect = 2.127e-11 Identity = 101/477 (21.17%), Postives = 190/477 (39.83%), Query Frame = 1
Query: 259 CLNDCRKDNPNHIAAIHNDSFLTYSMLNTKANIVAKNLIRSCNLEIPMNECLVGVLLDEGFERLYSIMACLKLGLVFVPLAKNRNSDLLKRIIDKCNLSIII----------------------------------HKKCSDIDLLSNDNSIQTVLMDTLLDESKIIDSFNLLPREYNPPQSREKTVVLLHTSGSSGTPKTIKIT------SAQLFNRLFWQWRNLPFKSNEIVCHRTGFMFVDNIVECLSCILSTVTMVIVSPTEACDIVKLAEVI-QKYSVSWILTVPSLLQKWLKQLDNYSDIXXXXXXXXXXXXXGEMLFPSLAKKCLATFNRNSCKLVNLYGSTEVCGDVCCQTLYSINDVRMNSKNK--------FLSVGTPISNNQVFFESNSDNEGEVIVIGKN--------VSPTCGLAFQTGDVGFIADKKLFICGRIDDMVKVNGKKIFTKDISTAVMVHSDVDNC 1518
C ++C P + TY + A+ ++ L+ C+ + + E +G+ G + I+ L++ +VP+ + L + KCNL I+ H K ++++L+ ND + S+ ++ E+ + + +LHTSG++G PK +++ + Q F LF +++ + F ++VE + S +++IV + KLA V+ + V+ + P+LL+++ QL S +L+ +SL + GE FPSL N ++ N+YG TEV T+Y I + +NS K F +GT + + + G+V + G+N V+ G TGD + D ++F GR D +K +GK++ + + V++C
Sbjct: 21 CFDECNNQLP---------VYYTYKTVVNAASELSNFLLLHCDFQ-GIRE--IGLYCQPGIDLPSWILGILQVPAAYVPIEPDSPPSLSTHFMKKCNLKYILVEKKQINKFKSFHETLLNYDTFTVEHNDLVLFRLHWKNTEVNLMLNDGKEKYEKEKIKSISSEHVNEEK--AEEHMDLRLKHCLAYVLHTSGTTGIPKIVRVPHKCIVPNIQHFRVLF------DITQEDVLFLASPLTFDPSVVEIFLALSSGASLLIVPTSVKLLPSKLASVLFSHHRVTVLQATPTLLRRFGSQLIK-STVLSATTSLRVLALGGEA-FPSLTVLRSWRGEGNKTQIFNVYGITEVSS---WATIYRIPEKTLNSTLKCELPVQLGFPLLGTVVEVRDTNGFTIQEGSGQVFLGGRNRVCFLDDEVTVPLGTMRATGDFVTVKDGEIFFLGRKDSQIKRHGKRLNIELVQQVAEELQQVESC 472
BLAST of Ss-alanyl conjugating enzyme vs. Ensembl Human
Match: AASDH (aminoadipate-semialdehyde dehydrogenase [Source:HGNC Symbol;Acc:HGNC:23993]) HSP 1 Score: 69.707 bits (169), Expect = 2.217e-11 Identity = 101/477 (21.17%), Postives = 190/477 (39.83%), Query Frame = 1
Query: 259 CLNDCRKDNPNHIAAIHNDSFLTYSMLNTKANIVAKNLIRSCNLEIPMNECLVGVLLDEGFERLYSIMACLKLGLVFVPLAKNRNSDLLKRIIDKCNLSIII----------------------------------HKKCSDIDLLSNDNSIQTVLMDTLLDESKIIDSFNLLPREYNPPQSREKTVVLLHTSGSSGTPKTIKIT------SAQLFNRLFWQWRNLPFKSNEIVCHRTGFMFVDNIVECLSCILSTVTMVIVSPTEACDIVKLAEVI-QKYSVSWILTVPSLLQKWLKQLDNYSDIXXXXXXXXXXXXXGEMLFPSLAKKCLATFNRNSCKLVNLYGSTEVCGDVCCQTLYSINDVRMNSKNK--------FLSVGTPISNNQVFFESNSDNEGEVIVIGKN--------VSPTCGLAFQTGDVGFIADKKLFICGRIDDMVKVNGKKIFTKDISTAVMVHSDVDNC 1518
C ++C P + TY + A+ ++ L+ C+ + + E +G+ G + I+ L++ +VP+ + L + KCNL I+ H K ++++L+ ND + S+ ++ E+ + + +LHTSG++G PK +++ + Q F LF +++ + F ++VE + S +++IV + KLA V+ + V+ + P+LL+++ QL S +L+ +SL + GE FPSL N ++ N+YG TEV T+Y I + +NS K F +GT + + + G+V + G+N V+ G TGD + D ++F GR D +K +GK++ + + V++C
Sbjct: 21 CFDECNNQLP---------VYYTYKTVVNAASELSNFLLLHCDFQ-GIRE--IGLYCQPGIDLPSWILGILQVPAAYVPIEPDSPPSLSTHFMKKCNLKYILVEKKQINKFKSFHETLLNYDTFTVEHNDLVLFRLHWKNTEVNLMLNDGKEKYEKEKIKSISSEHVNEEK--AEEHMDLRLKHCLAYVLHTSGTTGIPKIVRVPHKCIVPNIQHFRVLF------DITQEDVLFLASPLTFDPSVVEIFLALSSGASLLIVPTSVKLLPSKLASVLFSHHRVTVLQATPTLLRRFGSQLIK-STVLSATTSLRVLALGGEA-FPSLTVLRSWRGEGNKTQIFNVYGITEVSS---WATIYRIPEKTLNSTLKCELPVQLGFPLLGTVVEVRDTNGFTIQEGSGQVFLGGRNRVCFLDDEVTVPLGTMRATGDFVTVKDGEIFFLGRKDSQIKRHGKRLNIELVQQVAEELQQVESC 472
BLAST of Ss-alanyl conjugating enzyme vs. Ensembl Human
Match: AASDH (aminoadipate-semialdehyde dehydrogenase [Source:HGNC Symbol;Acc:HGNC:23993]) HSP 1 Score: 68.9366 bits (167), Expect = 3.797e-11 Identity = 101/477 (21.17%), Postives = 190/477 (39.83%), Query Frame = 1
Query: 259 CLNDCRKDNPNHIAAIHNDSFLTYSMLNTKANIVAKNLIRSCNLEIPMNECLVGVLLDEGFERLYSIMACLKLGLVFVPLAKNRNSDLLKRIIDKCNLSIII----------------------------------HKKCSDIDLLSNDNSIQTVLMDTLLDESKIIDSFNLLPREYNPPQSREKTVVLLHTSGSSGTPKTIKIT------SAQLFNRLFWQWRNLPFKSNEIVCHRTGFMFVDNIVECLSCILSTVTMVIVSPTEACDIVKLAEVI-QKYSVSWILTVPSLLQKWLKQLDNYSDIXXXXXXXXXXXXXGEMLFPSLAKKCLATFNRNSCKLVNLYGSTEVCGDVCCQTLYSINDVRMNSKNK--------FLSVGTPISNNQVFFESNSDNEGEVIVIGKN--------VSPTCGLAFQTGDVGFIADKKLFICGRIDDMVKVNGKKIFTKDISTAVMVHSDVDNC 1518
C ++C P + TY + A+ ++ L+ C+ + + E +G+ G + I+ L++ +VP+ + L + KCNL I+ H K ++++L+ ND + S+ ++ E+ + + +LHTSG++G PK +++ + Q F LF +++ + F ++VE + S +++IV + KLA V+ + V+ + P+LL+++ QL S +L+ +SL + GE FPSL N ++ N+YG TEV T+Y I + +NS K F +GT + + + G+V + G+N V+ G TGD + D ++F GR D +K +GK++ + + V++C
Sbjct: 21 CFDECNNQLP---------VYYTYKTVVNAASELSNFLLLHCDFQ-GIRE--IGLYCQPGIDLPSWILGILQVPAAYVPIEPDSPPSLSTHFMKKCNLKYILVEKKQINKFKSFHETLLNYDTFTVEHNDLVLFRLHWKNTEVNLMLNDGKEKYEKEKIKSISSEHVNEEK--AEEHMDLRLKHCLAYVLHTSGTTGIPKIVRVPHKCIVPNIQHFRVLF------DITQEDVLFLASPLTFDPSVVEIFLALSSGASLLIVPTSVKLLPSKLASVLFSHHRVTVLQATPTLLRRFGSQLIK-STVLSATTSLRVLALGGEA-FPSLTVLRSWRGEGNKTQIFNVYGITEVSS---WATIYRIPEKTLNSTLKCELPVQLGFPLLGTVVEVRDTNGFTIQEGSGQVFLGGRNRVCFLDDEVTVPLGTMRATGDFVTVKDGEIFFLGRKDSQIKRHGKRLNIELVQQVAEELQQVESC 472
BLAST of Ss-alanyl conjugating enzyme vs. Ensembl Human
Match: AASDH (aminoadipate-semialdehyde dehydrogenase [Source:HGNC Symbol;Acc:HGNC:23993]) HSP 1 Score: 63.929 bits (154), Expect = 1.438e-9 Identity = 73/289 (25.26%), Postives = 127/289 (43.94%), Query Frame = 1
Query: 721 LLHTSGSSGTPKTIKITSA------QLFNRLFWQWRNLPFKSNEIVCHRTGFMFVDNIVECLSCILSTVTMVIVSPTEACDIVKLAEVI-QKYSVSWILTVPSLLQKWLKQLDNYSDIXXXXXXXXXXXXXGEMLFPSLAKKCLATFNRNSCKLVNLYGSTEVCGDVCCQTLYSINDVRMNSKNK--------FLSVGTPISNNQVFFESNSDNEGEVIVIGKN--------VSPTCGLAFQTGDVGFIADKKLFICGRIDDMVKVNGKKIFTKDISTAVMVHSDVDNC 1518
+LHTSG++G PK +++ Q F LF +++ + F ++VE + S +++IV + KLA V+ + V+ + P+LL+++ QL S +L+ +SL + GE FPSL N ++ N+YG TEV T+Y I + +NS K F +GT + + + G+V + G+N V+ G TGD + D ++F GR D +K +GK++ + + V++C
Sbjct: 95 VLHTSGTTGIPKIVRVPHKCIVPNIQHFRVLF------DITQEDVLFLASPLTFDPSVVEIFLALSSGASLLIVPTSVKLLPSKLASVLFSHHRVTVLQATPTLLRRFGSQLIK-STVLSATTSLRVLALGGEA-FPSLTVLRSWRGEGNKTQIFNVYGITEVSS---WATIYRIPEKTLNSTLKCELPVQLGFPLLGTVVEVRDTNGFTIQEGSGQVFLGGRNRVCFLDDEVTVPLGTMRATGDFVTVKDGEIFFLGRKDSQIKRHGKRLNIELVQQVAEELQQVESC 372
BLAST of Ss-alanyl conjugating enzyme vs. Ensembl Human
Match: AASDH (aminoadipate-semialdehyde dehydrogenase [Source:HGNC Symbol;Acc:HGNC:23993]) HSP 1 Score: 63.1586 bits (152), Expect = 2.051e-9 Identity = 73/289 (25.26%), Postives = 128/289 (44.29%), Query Frame = 1
Query: 721 LLHTSGSSGTPKTIKIT------SAQLFNRLFWQWRNLPFKSNEIVCHRTGFMFVDNIVECLSCILSTVTMVIVSPTEACDIVKLAEVI-QKYSVSWILTVPSLLQKWLKQLDNYSDIXXXXXXXXXXXXXGEMLFPSLAKKCLATFNRNSCKLVNLYGSTEVCGDVCCQTLYSINDVRMNSKNK--------FLSVGTPISNNQVFFESNSDNEGEVIVIGKN--------VSPTCGLAFQTGDVGFIADKKLFICGRIDDMVKVNGKKIFTKDISTAVMVHSDVDNC 1518
+LHTSG++G PK +++ + Q F LF +++ + F ++VE + S +++IV + KLA V+ + V+ + P+LL+++ QL S +L+ +SL + GE FPSL N ++ N+YG TEV T+Y I + +NS K F +GT + + + G+V + G+N V+ G TGD + D ++F GR D +K +GK++ + + V++C
Sbjct: 42 VLHTSGTTGIPKIVRVPHKCIVPNIQHFRVLF------DITQEDVLFLASPLTFDPSVVEIFLALSSGASLLIVPTSVKLLPSKLASVLFSHHRVTVLQATPTLLRRFGSQLIK-STVLSATTSLRVLALGGEA-FPSLTVLRSWRGEGNKTQIFNVYGITEVSS---WATIYRIPEKTLNSTLKCELPVQLGFPLLGTVVEVRDTNGFTIQEGSGQVFLGGRNRVCFLDDEVTVPLGTMRATGDFVTVKDGEIFFLGRKDSQIKRHGKRLNIELVQQVAEELQQVESC 319
BLAST of Ss-alanyl conjugating enzyme vs. Ensembl Celegans
Match: acs-11 (Fatty Acid CoA Synthetase family [Source:UniProtKB/TrEMBL;Acc:Q20264]) HSP 1 Score: 52.373 bits (124), Expect = 1.197e-6 Identity = 98/453 (21.63%), Postives = 176/453 (38.85%), Query Frame = 1
Query: 163 VMNQLSNTSHCLYGENDSYEHCNNLATYFEMNCLNDCRKDNPNHIAAIHNDSFLTYSMLNTKANIVAKNLIRSCNLEIPMNECLVGVLLDEGFERLYSIMACLKLGLVFVPLAKNRNSDLLKRIIDKCNLSIIIHKKC-SDIDLLSNDNSIQTVLMDTLLDESKIIDSFNLLPR-EYNPPQSREKTVVLLHTSGSSGTPKTIKITSAQLFNRLFWQWRNLPFKSNEIVCHRTGFMFVDNIVECLSC-ILSTVTMVIVSPTEACDIVKLAEVIQKYSVSWILTVPSLLQKWLKQLDNYSDIXXXXXXXXXXXXXGEMLFPSLAKKCLATFNRNSCKLV-NLYGSTEVCGDVCCQTLYSINDVRMNSKNKFLSVGTPISNNQVFFESNSDNEGE--VIVIGKNVSP-------TCGLAFQTGDVGFI-ADKKLFICGRIDDMVKVNGKKIFTKDI 1479
++++L ++ H G + NN+ + ++ + +P+ + I D TY + A L N++ + + V LY +ACL++G +++P+ I SI++ C ++D + D I+ + D L E+ +++ ++ E + P S + +TSG++G PK +T L N R+ F N+ H F V + L C + S TM+ S E D +K + + + ++ VP+ + L + V SG L+ + F + +++ YG TE G + L N + K +VG + N E + I G +P T F+TGDVG + D L I GR DMV G ++ K++
Sbjct: 1 MLSRLVSSVHPQIGLRAASSAANNIVSQAQL-------QSDPSKLLFIDGDRKTTYGEFVKRTGQYATALTEKYNIK-KGDRVMARVSKTTDTAALY--IACLQIGALYIPVNPGYTESEAAHYIKDATPSILV--SCNEELDKVFRDK-IRVINEDKLASEAGSLNACTMIEHVEKSDPAS------VCYTSGTTGLPKGAILTHGSLSNNAHDIVRDWGFTGNDYNLHALPFYHVHGLYYSLHCSLFSHSTMIWRSKFEVEDCIKYMK-----NATVMMGVPTFFSRLLAS----KNFNKEAFGNVRVFISGSA---PLSVSTIEEFRERTGQVILERYGMTE-AGVMTTNPL--------NGERKAGTVGPAVQGVGCRIAKNGGIEVKTNAIFAGYWKNPKKTAEEFTEDGWFKTGDVGHLDEDGYLTIGGRSKDMVITGGLNVYPKEL 413
BLAST of Ss-alanyl conjugating enzyme vs. Ensembl Celegans
Match: acs-11 (Fatty Acid CoA Synthetase family [Source:UniProtKB/TrEMBL;Acc:Q20264]) HSP 1 Score: 52.373 bits (124), Expect = 1.197e-6 Identity = 98/453 (21.63%), Postives = 176/453 (38.85%), Query Frame = 1
Query: 163 VMNQLSNTSHCLYGENDSYEHCNNLATYFEMNCLNDCRKDNPNHIAAIHNDSFLTYSMLNTKANIVAKNLIRSCNLEIPMNECLVGVLLDEGFERLYSIMACLKLGLVFVPLAKNRNSDLLKRIIDKCNLSIIIHKKC-SDIDLLSNDNSIQTVLMDTLLDESKIIDSFNLLPR-EYNPPQSREKTVVLLHTSGSSGTPKTIKITSAQLFNRLFWQWRNLPFKSNEIVCHRTGFMFVDNIVECLSC-ILSTVTMVIVSPTEACDIVKLAEVIQKYSVSWILTVPSLLQKWLKQLDNYSDIXXXXXXXXXXXXXGEMLFPSLAKKCLATFNRNSCKLV-NLYGSTEVCGDVCCQTLYSINDVRMNSKNKFLSVGTPISNNQVFFESNSDNEGE--VIVIGKNVSP-------TCGLAFQTGDVGFI-ADKKLFICGRIDDMVKVNGKKIFTKDI 1479
++++L ++ H G + NN+ + ++ + +P+ + I D TY + A L N++ + + V LY +ACL++G +++P+ I SI++ C ++D + D I+ + D L E+ +++ ++ E + P S + +TSG++G PK +T L N R+ F N+ H F V + L C + S TM+ S E D +K + + + ++ VP+ + L + V SG L+ + F + +++ YG TE G + L N + K +VG + N E + I G +P T F+TGDVG + D L I GR DMV G ++ K++
Sbjct: 1 MLSRLVSSVHPQIGLRAASSAANNIVSQAQL-------QSDPSKLLFIDGDRKTTYGEFVKRTGQYATALTEKYNIK-KGDRVMARVSKTTDTAALY--IACLQIGALYIPVNPGYTESEAAHYIKDATPSILV--SCNEELDKVFRDK-IRVINEDKLASEAGSLNACTMIEHVEKSDPAS------VCYTSGTTGLPKGAILTHGSLSNNAHDIVRDWGFTGNDYNLHALPFYHVHGLYYSLHCSLFSHSTMIWRSKFEVEDCIKYMK-----NATVMMGVPTFFSRLLAS----KNFNKEAFGNVRVFISGSA---PLSVSTIEEFRERTGQVILERYGMTE-AGVMTTNPL--------NGERKAGTVGPAVQGVGCRIAKNGGIEVKTNAIFAGYWKNPKKTAEEFTEDGWFKTGDVGHLDEDGYLTIGGRSKDMVITGGLNVYPKEL 413
BLAST of Ss-alanyl conjugating enzyme vs. Ensembl Fly
Match: e (gene:FBgn0000527 transcript:FBtr0084106) HSP 1 Score: 154.836 bits (390), Expect = 3.451e-38 Identity = 181/839 (21.57%), Postives = 361/839 (43.03%), Query Frame = 1
Query: 325 TYSMLNTKANIVAKNLIRSCNLEIPM----NECLVGVLLDEGFERLYSIMACLKLGLVFVPLAKNRNSDLLKRIIDKCNLSIIIHKKCSDIDLLSNDNSIQTVLMDTLLDESKIIDSFNLLPREYNPPQSREKTVVLLHTSGSSGTPKTIKITSAQLFNRLFWQWRNLPFKSNEIV-CHRTGFMFVDNIVECLSCILSTVTMVIVSPTEACDIVKLAEVIQKYSVSWILTVPSLLQKWLK--QLDNYSDIXXXXXXXXXXXXXGEMLFPSLAKKCLATFNRNSCKLVNLYGSTEVCGDVCCQTLYSINDVRMNSKNKFLSVGTPISNNQVF-----FESNSDNE-GEVIVIGKN--------------------VSPTCGLAFQTGDVGFIADKKLFICGRIDDMVKVNGKKI----FTKDISTAVMVHSDVDNCYTIQLIISGRPQLVSFFTTKTEIGNKTKTKMEINNILMNHSLNVCLPRLEYIKSIPIQPQSMKPDKKMLCKIAKKILSENRRMKTKLTHGKHXXXXXXXXXXXXXXXXXXNSQKHYIYEILAKHLSLLPDEIDDSMKFYDIGGDSLTIVICIADLNRKGFSCTTEVFFHNHSIGELVECILEQSNSKIRNNSDYKLFKYDMKN-----DFTVEQIQYENKTEIIEFLVENFYTKELVVRYLNTPV--KVFVEVVSEIFDLSLRSGCSFCIRNSISKSIVGLQLSEDS--SYEIPNTSQLYKLDEENSLDCFLQNCKPRIAE-FLKTPK-----LNVSVVALSGTLNKSIVIQLLYFIEKHTIDLAQCKNYKTIETINTSEATKKICSELNYRLIKSTSMSHFLN 2685
+Y +N +AN A+ L+ + + +V V + + +++A K G ++P+ + ++ + I+ + +++I D ++ T L +S + NLL E + ++L+TSGS+G PK +++ + NRL WQW P+ +NE V +T FVD+I E ++ + +++V D +L ++++Y + ++ VP+LL+ L +++ LL +L V SGE L SLA F+ +L N YGSTEV GDV T ++ + S + +G P+SN V+ + + E GE+ G N V ++TGD G + + + GR D VK+ G ++ K+++ +V + CY + +++F + + T+ +ME L + + P++ ++ +P+ K D++ L K + + L + L D LA H FY++GG+SL + + L KG++ F ++GE++E K+ N D + + N ++ E++ E+I+ +V +FY K + ++L V + +++++I+++ + SF + ++ + I+G L+ D+ E+ S+L + E FL+ C+ I + +L PK L+ ++ + LN I ++F+E + +A+ K + I T NTS T+++ +Y+ + + ++ +++
Sbjct: 56 SYRQMNERANRAARLLVAETHGRFLQPNSDGDFIVAVCMQPSEGLVTTLLAIWKAGGAYLPIDPSFPANRIHHILLEAKPTLVIRDDDIDAGRFQGTPTLSTT---ELYAKSLQLAGSNLLSEEMLR-GGNDHIAIVLYTSGSTGVPKGVRLPHESILNRLQWQWATFPYTANEAVSVFKTALTFVDSIAELWGPLMCGLAILVVPKAVTKDPQRLVALLERYKIRRLVLVPTLLRSLLMYLKMEGGGAAQKLLYNLQIWVCSGEPLSVSLASSFFDYFDEGVHRLYNFYGSTEVLGDV---TYFACESKKQLSLYDNVPIGIPLSNTVVYLLDADYRPVKNGEIGEIFASGLNLAAGYVNGRDPERFLENPLAVEKKYARLYRTGDYGSLKNGSIMYEGRTDSQVKIRGHRVDLSEVEKNVAELPLVDKAIVLCYHAGQVDQA---ILAFVKLRDDAPMVTEMQMEAR--LKDKLADYMTPQVVILEHVPLLVNG-KVDRQALLKTYETANNNEGDSSIVLDFDYSQVPEDLKLTARDLFETVGGVIGRSTRATLAPH-----------SNFYELGGNSLNSIFTVTLLREKGYNIGISEFIAAKNLGEIIE--------KMAANHDAVQLEEESLNACPHLKMEAVPLRLEHRQEVIDIIVASFYNKADLEQWLKPGVLRTDYSDILNDIWNVLVERDLSFVVYDTNTDRIIGTALNFDARNEPEVDIKSKLLIVFE------FLEFCEGPIRDNYL--PKGLNQILHSFMMGTAEKLNPRENIACMHFMEHEVLRVAREKQFAGIFTTNTSPLTQQLADVYHYKTLLNFQVNEYVH 854
BLAST of Ss-alanyl conjugating enzyme vs. Ensembl Zebrafish
Match: aasdh (aminoadipate-semialdehyde dehydrogenase [Source:NCBI gene;Acc:570338]) HSP 1 Score: 53.5286 bits (127), Expect = 1.335e-6 Identity = 70/293 (23.89%), Postives = 130/293 (44.37%), Query Frame = 1
Query: 697 QSREKTVVLLHTSGSSGTPKTIKITSAQLFNRLFWQWRNLPFKSNEIVCHRTGFMFVDNIVECLSCILSTVTMVIVSPTEACDIVKLAEVIQKYSVSWIL-TVPSLLQKWLKQLDNYSDIXXXXXXXXXXXXXGEMLFPS--LAKKCLATFNRNSCKLVNLYGSTEVCGDVCCQTLYSINDVRM---NSKNKFLSVGTPISNN--QVFFESN---SDNEGEVIVIGKNV--------SPTCGLAFQTGDVGFIADKKLFICGRIDDMVKVNGKKIFTKDISTAVMVHSDVDNC 1518
Q E +LHTSG++G PK +K+ + + ++V + F ++VE + S ++IV +LA V+ K + + +L P+L++++ K + ++L+ SSL + GE PS L K NR + NLYG+TEV C + Y + D + + + + +G P+ + +V E+ ++ EG++ + G+N + G TGD + + L+ GR D +VK G+++ + + S V+ C
Sbjct: 170 QQGEPLAYILHTSGTTGLPKIVKVPHRCIVPNIIHLRSVFKMTPEDVVFLSSPLTFDPSVVEVFLALSSGACLLIVPSAVKKMPRRLAHVLFKRNTTTVLQATPTLVRRFGKVVLQ-EEVLSADSSLRILAFGGEPC-PSLNLVKSWRQEGNRT--HIYNLYGTTEVS---CWASWYKVPDEHLCLEDITDAPVPLGEPMLDTVMEVRDETGHLVTEGEGQLFIGGQNRVCLLDDEETVVKGTMRATGDWVQVQNSNLYFLGRKDRLVKRFGQRVHLDALQQMIESFSGVEAC 455
BLAST of Ss-alanyl conjugating enzyme vs. Ensembl Zebrafish
Match: aasdh (aminoadipate-semialdehyde dehydrogenase [Source:NCBI gene;Acc:570338]) HSP 1 Score: 53.5286 bits (127), Expect = 1.386e-6 Identity = 70/293 (23.89%), Postives = 130/293 (44.37%), Query Frame = 1
Query: 697 QSREKTVVLLHTSGSSGTPKTIKITSAQLFNRLFWQWRNLPFKSNEIVCHRTGFMFVDNIVECLSCILSTVTMVIVSPTEACDIVKLAEVIQKYSVSWIL-TVPSLLQKWLKQLDNYSDIXXXXXXXXXXXXXGEMLFPS--LAKKCLATFNRNSCKLVNLYGSTEVCGDVCCQTLYSINDVRM---NSKNKFLSVGTPISNN--QVFFESN---SDNEGEVIVIGKNV--------SPTCGLAFQTGDVGFIADKKLFICGRIDDMVKVNGKKIFTKDISTAVMVHSDVDNC 1518
Q E +LHTSG++G PK +K+ + + ++V + F ++VE + S ++IV +LA V+ K + + +L P+L++++ K + ++L+ SSL + GE PS L K NR + NLYG+TEV C + Y + D + + + + +G P+ + +V E+ ++ EG++ + G+N + G TGD + + L+ GR D +VK G+++ + + S V+ C
Sbjct: 170 QQGEPLAYILHTSGTTGLPKIVKVPHRCIVPNIIHLRSVFKMTPEDVVFLSSPLTFDPSVVEVFLALSSGACLLIVPSAVKKMPRRLAHVLFKRNTTTVLQATPTLVRRFGKVVLQ-EEVLSADSSLRILAFGGEPC-PSLNLVKSWRQEGNRT--HIYNLYGTTEVS---CWASWYKVPDEHLCLEDITDAPVPLGEPMLDTVMEVRDETGHLVTEGEGQLFIGGQNRVCLLDDEETVVKGTMRATGDWVQVQNSNLYFLGRKDRLVKRFGQRVHLDALQQMIESFSGVEAC 455
BLAST of Ss-alanyl conjugating enzyme vs. Ensembl Xenopus
Match: rapsn (receptor associated protein of the synapse [Source:Xenbase;Acc:XB-GENE-990995]) HSP 1 Score: 71.633 bits (174), Expect = 5.071e-12 Identity = 103/487 (21.15%), Postives = 213/487 (43.74%), Query Frame = 1
Query: 319 FLTYSMLNTKANIVAKNLIRSCNLEIPMNECLVGVLLDEGFERLYSIMACLKLGLVFVPLAKNRNSDLLKRIIDKCNLS-IIIHKKCSDIDLLSN--DNSIQTVLMDTLLDESKIIDSFNLLPREYNPPQSREKTVVLLHTSGSSGTPKTIKITSAQLFNRLFWQWRNLPFKSNEIVCHRTGFMFVDNIVECL------SCILSTVTMVIVSPTEACDIVKLAEVIQKYSVSWILTVPSLLQKWLKQLDNYSDIXXXXXXXXXXXXXGEMLFPSLAKKCLATFNRNSCKLVNLYGSTEVCGDVCCQTLYSINDVRMNSKNKF------LSVGTPISNNQVFFESNS-----DNEGEVIVIGKN---------VSPTCGLAFQTGDVGFIADKKLFICGRIDDMVKVNGKKIFTKDISTAVMVHSDVDNCYTIQLIISGRPQLVSFFTTKTEIGNKTKTKMEINNILMNHSLNVCLP-RLEYIKSIPI 1689
+ TYS + A +A L C C +G+ + G I+ L++ + P+ + +SD+ ++++K N+ +++HK ++ S I V+ + +E N++ +E+ + + +LHTSG++G PK + + + ++ ++++ + F +++E +C+L + + P + CDI + ++ V+ + P+ L+++ + S +L+ +SL + GE FPS++ N +L+NLYG TEV T Y I + +NS N F + +G P+ V ++ + EG+V++ G+ + P G +TGD + D +++ GR D+ +K +GK++ + + + + V+ C + QLV F I N K + L +H L+ +P L ++++P+
Sbjct: 26 YYTYSKMMRLAEELALFLKSHCR---HTETCKIGLYCNPGINLPSYIIGILQVPAAYFPVDPSNSSDVSIKLLEKSNVEYMLVHKDMVEVSATSVFLILCIGKVMFNDTPEEKH---KENVMYKEHMDMREKHCLAYILHTSGTTGAPKIVSVPHKCIVPNIYHLRSIFAISPDDLLFLASPLTFDPSVIELFLALSSGACLLVVPNPIKMMPHKLCDI-----LFHQHRVTVLQATPTFLRRFGSR-SIRSSVLSKGTSLRVLALGGEP-FPSVSVLRSWREPGNRTRLINLYGITEVSS---WATYYEITEQMINS-NLFYDIFSCVPLGFPLQGTIVEVQNEKGYKIDEGEGQVLLGGRERICFLDNEFILPY-GTMRKTGDWVTLKDGEMYFLGRKDNQIKRHGKRLNIEYVQQSAESLAHVEVCTALWF---QEKQLVMFV-----IPNGPLEKKFLWKELQSHLLSYAVPDDLVLVETLPL 486
BLAST of Ss-alanyl conjugating enzyme vs. Ensembl Xenopus
Match: rapsn (receptor associated protein of the synapse [Source:Xenbase;Acc:XB-GENE-990995]) HSP 1 Score: 67.3958 bits (163), Expect = 1.137e-10 Identity = 103/496 (20.77%), Postives = 210/496 (42.34%), Query Frame = 1
Query: 319 FLTYSMLNTKANIVAKNLIRSCNLEIPMNECLVGVLLDEGFERLYSIMACLKLGLVFVPLAKNRNSDLLKRIID--------------KCNLSIIIHKKCSDIDLLSNDNSIQTVLMDTLLDESKIIDSFNLLPREYNPPQSREKTVVLLHTSGSSGTPKTIKITSAQLFNRLFWQWRNLPFKSNEIVCHRTGFMFVDNIVECL------SCILSTVTMVIVSPTEACDIVKLAEVIQKYSVSWILTVPSLLQKWLKQLDNYSDIXXXXXXXXXXXXXGEMLFPSLAKKCLATFNRNSCKLVNLYGSTEVCGDVCCQTLYSIND-VRMNSKNKFLS---VGTPISNNQVFFESNS-----DNEGEVIVIGKN---------VSPTCGLAFQTGDVGFIADKKLFICGRIDDMVKVNGKKIFTKDISTAVMVHSDVDNCYTIQLIISGRPQLVSFFTTKTEIGNKTKTKMEINNILMNHSLNVCLP-RLEYIKSIPI 1689
+ TYS + A +A L C C +G+ + G I+ L++ + P+ + +SD++ ++ + + S I H + ++ N +I L D + N++ +E+ + + +LHTSG++G PK + + + ++ ++++ + F +++E +C+L + + P + CDI + ++ V+ + P+ L+++ + S +L+ +SL + GE FPS++ N +L+NLYG TEV T Y I + V+ NS+ K S +G P+ V ++ + EG+V++ G+ + P G +TGD + D +++ GR D+ +K +GK++ + + + + V+ C + QLV F I N K + L +H L+ +P L ++++P+
Sbjct: 26 YYTYSKMMRLAEELALFLKSHCR---HTETCKIGLYCNPGINLPSYIIGILQVPAAYFPVDPSNSSDVMTNLLSLTLQNFKLQFPGFVELDSSSIQHINVNLFEVKRNKKAIVRGLHRHQNDTPEEKHKENVMYKEHMDMREKHCLAYILHTSGTTGAPKIVSVPHKCIVPNIYHLRSIFAISPDDLLFLASPLTFDPSVIELFLALSSGACLLVVPNPIKMMPHKLCDI-----LFHQHRVTVLQATPTFLRRFGSR-SIRSSVLSKGTSLRVLALGGEP-FPSVSVLRSWREPGNRTRLINLYGITEVSS---WATYYEITESVQNNSRGKEESCVPLGFPLQGTIVEVQNEKGYKIDEGEGQVLLGGRERICFLDNEFILPY-GTMRKTGDWVTLKDGEMYFLGRKDNQIKRHGKRLNIEYVQQSAESLAHVEVCTALWF---QEKQLVMFV-----IPNGPLEKKFLWKELQSHLLSYAVPDDLVLVETLPL 499
BLAST of Ss-alanyl conjugating enzyme vs. Ensembl Xenopus
Match: rapsn (receptor associated protein of the synapse [Source:Xenbase;Acc:XB-GENE-990995]) HSP 1 Score: 63.929 bits (154), Expect = 1.228e-9 Identity = 98/492 (19.92%), Postives = 205/492 (41.67%), Query Frame = 1
Query: 319 FLTYSMLNTKANIVAKNLIRSCNLEIPMNECLVGVLLDEGFERLYSIMACLKLGLVFVPLAKNRNSDLLKRIID--------------KCNLSIIIHKKCSDIDLLSNDNSIQTVLMDTLLDESKIIDSFNLLPREYNPPQSREKTVVLLHTSGSSGTPKTIKITSAQLFNRLFWQWRNLPFKSNEIVCHRTGFMFVDNIVECL------SCILSTVTMVIVSPTEACDIVKLAEVIQKYSVSWILTVPSLLQKWLKQLDNYSDIXXXXXXXXXXXXXGEMLFPSLAKKCLATFNRNSCKLVNLYGSTEVCGDVCCQTLYSINDVRMNSKNKFLSVGTPISNNQVFFESNS-----DNEGEVIVIGKN---------VSPTCGLAFQTGDVGFIADKKLFICGRIDDMVKVNGKKIFTKDISTAVMVHSDVDNCYTIQLIISGRPQLVSFFTTKTEIGNKTKTKMEINNILMNHSLNVCLP-RLEYIKSIPI 1689
+ TYS + A +A L C C +G+ + G I+ L++ + P+ + +SD++ ++ + + S I H + ++ N +I L D + N++ +E+ + + +LHTSG++G PK + + + ++ ++++ + F +++E +C+L + + P + CDI + ++ V+ + P+ L+++ + S +L+ +SL + GE FPS++ N +L+NLYG TEV T Y I + + + +G P+ V ++ + EG+V++ G+ + P G +TGD + D +++ GR D+ +K +GK++ + + + + V+ C + QLV F I N K + L +H L+ +P L ++++P+
Sbjct: 26 YYTYSKMMRLAEELALFLKSHCR---HTETCKIGLYCNPGINLPSYIIGILQVPAAYFPVDPSNSSDVMTNLLSLTLQNFKLQFPGFVELDSSSIQHINVNLFEVKRNKKAIVRGLHRHQNDTPEEKHKENVMYKEHMDMREKHCLAYILHTSGTTGAPKIVSVPHKCIVPNIYHLRSIFAISPDDLLFLASPLTFDPSVIELFLALSSGACLLVVPNPIKMMPHKLCDI-----LFHQHRVTVLQATPTFLRRFGSR-SIRSSVLSKGTSLRVLALGGEP-FPSVSVLRSWREPGNRTRLINLYGITEVSS---WATYYEITE-----EESCVPLGFPLQGTIVEVQNEKGYKIDEGEGQVLLGGRERICFLDNEFILPY-GTMRKTGDWVTLKDGEMYFLGRKDNQIKRHGKRLNIEYVQQSAESLAHVEVCTALWF---QEKQLVMFV-----IPNGPLEKKFLWKELQSHLLSYAVPDDLVLVETLPL 490
BLAST of Ss-alanyl conjugating enzyme vs. Ensembl Xenopus
Match: rapsn (receptor associated protein of the synapse [Source:Xenbase;Acc:XB-GENE-990995]) HSP 1 Score: 57.3806 bits (137), Expect = 1.312e-7 Identity = 77/352 (21.88%), Postives = 155/352 (44.03%), Query Frame = 1
Query: 703 REKTVV--LLHTSGSSGTPKTIKITSAQLFNRLFWQWRNLPFKSNEIVCHRTGFMFVDNIVECL------SCILSTVTMVIVSPTEACDIVKLAEVIQKYSVSWILTVPSLLQKWLKQLDNYSDIXXXXXXXXXXXXXGEMLFPSLAKKCLATFNRNSCKLVNLYGSTEVCGDVCCQTLYSINDVRMNSKNKFLSVGTPISNNQVFFESNS-----DNEGEVIVIGKN---------VSPTCGLAFQTGDVGFIADKKLFICGRIDDMVKVNGKKIFTKDISTAVMVHSDVDNCYTIQLIISGRPQLVSFFTTKTEIGNKTKTKMEINNILMNHSLNVCLP-RLEYIKSIPI 1689
REK + +LHTSG++G PK + + + ++ ++++ + F +++E +C+L + + P + CDI+ ++ V+ + P+ L+++ + S +L+ +SL + GE FPS++ N +L+NLYG TEV T Y I + + + +G P+ V ++ + EG+V++ G+ + P G +TGD + D +++ GR D+ +K +GK++ + + + + V+ C + QLV F I N K + L +H L+ +P L ++++P+
Sbjct: 9 REKHCLAYILHTSGTTGAPKIVSVPHKCIVPNIYHLRSIFAISPDDLLFLASPLTFDPSVIELFLALSSGACLLVVPNPIKMMPHKLCDIL-----FHQHRVTVLQATPTFLRRFGSR-SIRSSVLSKGTSLRVLALGGEP-FPSVSVLRSWREPGNRTRLINLYGITEVSS---WATYYEITE-----EESCVPLGFPLQGTIVEVQNEKGYKIDEGEGQVLLGGRERICFLDNEFILPY-GTMRKTGDWVTLKDGEMYFLGRKDNQIKRHGKRLNIEYVQQSAESLAHVEVCTALWF---QEKQLVMFV-----IPNGPLEKKFLWKELQSHLLSYAVPDDLVLVETLPL 336
BLAST of Ss-alanyl conjugating enzyme vs. Ensembl Mouse
Match: Aasdh (aminoadipate-semialdehyde dehydrogenase [Source:MGI Symbol;Acc:MGI:2442517]) HSP 1 Score: 85.1149 bits (209), Expect = 2.088e-16 Identity = 140/621 (22.54%), Postives = 251/621 (40.42%), Query Frame = 1
Query: 325 TYSMLNTKANIVAKNLIRSCNLEIPMNECLVGVLLDEGFERLYSIMACLKLGLVFVPLAKNRNSDLLKRIIDKCNLSIII-------------------------HKKCSDIDLLSNDNSIQTVLMDTLLDESKIIDSFNLLPREYNPPQSREKTV-------VLLHTSGSSGTPKTIKITSA------QLFNRLFWQWRNLPFKSNEIVCHRTGFMFVDNIVECL------SCILSTVTMVIVSPTEACDIVKLAEVIQKYSVSWILTVPSLLQKWLKQLDNYSDIXXXXXXXXXXXXXGEMLFPSLAKKCLATFNRNSCKLVNLYGSTEVCGDVCCQTLYSINDVRMNSKNKFLS---VGTPISNNQVFFESNS-----DNEGEVIVIGKN--------VSPTCGLAFQTGDVGFIADKKLFICGRIDDMVKVNGKKIFTKDISTAVMVHSDVDNCYTIQLIISGRPQLVSFFTTKTEIGNKTKTKMEINNILMNHSLNVCLPRLEYIKSIPIQPQSMKPDKKMLCKIAKKILSENRRMKTKLTHGKHXXXXXXXXXXXXXXXXXXNSQKHYIYEILAKHLSLLPDEIDDSMK------FYDIGGDSLTIVICIADLNR 1989
+Y L + A+ ++ LI C+ + E +G+ G I+ L++ + P+ + L + KC+L ++ HK + L D + TVL D D+ K+ D + + E P+ + +LHTSG++GTPK +++ A Q F LF +I+ + F ++VE +C+L T V V P++ DI + ++ V+ + P+LL+++ +L S +L+ +SL + GE FPSL N ++ N+YG TEV T Y I + +NS K S +G+P+ + + + G+V + GKN ++ G TGD + D ++F GR D +K +GK++ +I+ V ++ + + + +L+ F +K ++ K E+ L H+L + I ++P K D L KI +S R + HGK L + Y++ K + LP++ +D++K F D GGDSL + ++++ R
Sbjct: 34 SYKALLSAASELSHFLIAHCDFG-GIRE--IGLYCQPGINLPSWILGILQVPAAYAPIDPDSPPSLSTYFMKKCDLKYVLVEKQQLSKFKSSHETVLNYDTVSVEHKDLALFRLHWEDGRVSTVLGDRA-DQHKVTDREDRVSAESRTPEKEHMDMRHDGCLAYVLHTSGTTGTPKIVRVPHACILPNIQHFRSLF------DITQEDILFLASPLTFDPSVVEIFVSLSSGACLLIVPTSVKVLPSKLADI-----LFSRHRVTVLQATPTLLRRFGSELIK-STVLSAHTSLRVLALGGEA-FPSLTILKSWRGKGNRTQIFNIYGITEVS---SWATFYRIPEEILNSAVKHESPVQLGSPLLGTVIEVRDQNGSPVLEGTGQVFLGGKNRVCFLDDEMTVPLGTMRATGDFVTVKDGEIFFLGRKDSQIKRHGKRL---NIALVQQVAEELRQVESCAVTWYNQERLILFIVSKVDLVKDCIFK-ELQKHLPAHALP---DDMVLIDTLPFTCHG-KVDVSELNKIYLDYISSQPRNE---LHGKEELW----------------GKLQYLW----KSILCLPEDPEDTLKVPANSVFLDSGGDSLKSMRLLSEIER 603
BLAST of Ss-alanyl conjugating enzyme vs. Ensembl Mouse
Match: Aasdh (aminoadipate-semialdehyde dehydrogenase [Source:MGI Symbol;Acc:MGI:2442517]) HSP 1 Score: 83.9593 bits (206), Expect = 6.435e-16 Identity = 142/623 (22.79%), Postives = 253/623 (40.61%), Query Frame = 1
Query: 325 TYSMLNTKANIVAKNLIRSCNLEIPMNECLVGVLLDEGFERLYSIMACLKLGLVFVPLAKNRNSDLLKRIIDKCNLSIII-------------------------HKKCSDIDLLSNDNSIQTVLMDTLLDESKIIDSFNLLPREYNPPQSREKTV-------VLLHTSGSSGTPKTIKITSA------QLFNRLFWQWRNLPFKSNEIVCHRTGFMFVDNIVECL------SCILSTVTMVIVSPTEACDIVKLAEVIQKYSVSWILTVPSLLQKWLKQLDNYSDIXXXXXXXXXXXXXGEMLFPSLA--KKCLATFNRNSCKLVNLYGSTEVCGDVCCQTLYSINDVRMNSKNKFLS---VGTPISNNQVFFESNS-----DNEGEVIVIGKN--------VSPTCGLAFQTGDVGFIADKKLFICGRIDDMVKVNGKKIFTKDISTAVMVHSDVDNCYTIQLIISGRPQLVSFFTTKTEIGNKTKTKMEINNILMNHSLNVCLPRLEYIKSIPIQPQSMKPDKKMLCKIAKKILSENRRMKTKLTHGKHXXXXXXXXXXXXXXXXXXNSQKHYIYEILAKHLSLLPDEIDDSMK------FYDIGGDSLTIVICIADLNR 1989
+Y L + A+ ++ LI C+ + E +G+ G I+ L++ + P+ + L + KC+L ++ HK + L D + TVL D D+ K+ D + + E P+ + +LHTSG++GTPK +++ A Q F LF +I+ + F ++VE +C+L T V V P++ DI + ++ V+ + P+LL+++ +L S +L+ +SL + GE FPSL K NR ++ N+YG TEV T Y I + +NS K S +G+P+ + + + G+V + GKN ++ G TGD + D ++F GR D +K +GK++ +I+ V ++ + + + +L+ F +K ++ K E+ L H+L + I ++P K D L KI +S R + HGK L + Y++ K + LP++ +D++K F D GGDSL + ++++ R
Sbjct: 34 SYKALLSAASELSHFLIAHCDFG-GIRE--IGLYCQPGINLPSWILGILQVPAAYAPIDPDSPPSLSTYFMKKCDLKYVLVEKQQLSKFKSSHETVLNYDTVSVEHKDLALFRLHWEDGRVSTVLGDRA-DQHKVTDREDRVSAESRTPEKEHMDMRHDGCLAYVLHTSGTTGTPKIVRVPHACILPNIQHFRSLF------DITQEDILFLASPLTFDPSVVEIFVSLSSGACLLIVPTSVKVLPSKLADI-----LFSRHRVTVLQATPTLLRRFGSELIK-STVLSAHTSLRVLALGGEA-FPSLTILKSWRGKGNRT--QIFNIYGITEVSS---WATFYRIPEEILNSAVKHESPVQLGSPLLGTVIEVRDQNGSPVLEGTGQVFLGGKNRVCFLDDEMTVPLGTMRATGDFVTVKDGEIFFLGRKDSQIKRHGKRL---NIALVQQVAEELRQVESCAVTWYNQERLILFIVSKVDLVKDCIFK-ELQKHLPAHALP---DDMVLIDTLPFTCHG-KVDVSELNKIYLDYISSQPRNE---LHGKEELW----------------GKLQYLW----KSILCLPEDPEDTLKVPANSVFLDSGGDSLKSMRLLSEIER 603
BLAST of Ss-alanyl conjugating enzyme vs. Ensembl Mouse
Match: Aasdh (aminoadipate-semialdehyde dehydrogenase [Source:MGI Symbol;Acc:MGI:2442517]) HSP 1 Score: 83.9593 bits (206), Expect = 6.435e-16 Identity = 142/623 (22.79%), Postives = 253/623 (40.61%), Query Frame = 1
Query: 325 TYSMLNTKANIVAKNLIRSCNLEIPMNECLVGVLLDEGFERLYSIMACLKLGLVFVPLAKNRNSDLLKRIIDKCNLSIII-------------------------HKKCSDIDLLSNDNSIQTVLMDTLLDESKIIDSFNLLPREYNPPQSREKTV-------VLLHTSGSSGTPKTIKITSA------QLFNRLFWQWRNLPFKSNEIVCHRTGFMFVDNIVECL------SCILSTVTMVIVSPTEACDIVKLAEVIQKYSVSWILTVPSLLQKWLKQLDNYSDIXXXXXXXXXXXXXGEMLFPSLA--KKCLATFNRNSCKLVNLYGSTEVCGDVCCQTLYSINDVRMNSKNKFLS---VGTPISNNQVFFESNS-----DNEGEVIVIGKN--------VSPTCGLAFQTGDVGFIADKKLFICGRIDDMVKVNGKKIFTKDISTAVMVHSDVDNCYTIQLIISGRPQLVSFFTTKTEIGNKTKTKMEINNILMNHSLNVCLPRLEYIKSIPIQPQSMKPDKKMLCKIAKKILSENRRMKTKLTHGKHXXXXXXXXXXXXXXXXXXNSQKHYIYEILAKHLSLLPDEIDDSMK------FYDIGGDSLTIVICIADLNR 1989
+Y L + A+ ++ LI C+ + E +G+ G I+ L++ + P+ + L + KC+L ++ HK + L D + TVL D D+ K+ D + + E P+ + +LHTSG++GTPK +++ A Q F LF +I+ + F ++VE +C+L T V V P++ DI + ++ V+ + P+LL+++ +L S +L+ +SL + GE FPSL K NR ++ N+YG TEV T Y I + +NS K S +G+P+ + + + G+V + GKN ++ G TGD + D ++F GR D +K +GK++ +I+ V ++ + + + +L+ F +K ++ K E+ L H+L + I ++P K D L KI +S R + HGK L + Y++ K + LP++ +D++K F D GGDSL + ++++ R
Sbjct: 34 SYKALLSAASELSHFLIAHCDFG-GIRE--IGLYCQPGINLPSWILGILQVPAAYAPIDPDSPPSLSTYFMKKCDLKYVLVEKQQLSKFKSSHETVLNYDTVSVEHKDLALFRLHWEDGRVSTVLGDRA-DQHKVTDREDRVSAESRTPEKEHMDMRHDGCLAYVLHTSGTTGTPKIVRVPHACILPNIQHFRSLF------DITQEDILFLASPLTFDPSVVEIFVSLSSGACLLIVPTSVKVLPSKLADI-----LFSRHRVTVLQATPTLLRRFGSELIK-STVLSAHTSLRVLALGGEA-FPSLTILKSWRGKGNRT--QIFNIYGITEVSS---WATFYRIPEEILNSAVKHESPVQLGSPLLGTVIEVRDQNGSPVLEGTGQVFLGGKNRVCFLDDEMTVPLGTMRATGDFVTVKDGEIFFLGRKDSQIKRHGKRL---NIALVQQVAEELRQVESCAVTWYNQERLILFIVSKVDLVKDCIFK-ELQKHLPAHALP---DDMVLIDTLPFTCHG-KVDVSELNKIYLDYISSQPRNE---LHGKEELW----------------GKLQYLW----KSILCLPEDPEDTLKVPANSVFLDSGGDSLKSMRLLSEIER 603
BLAST of Ss-alanyl conjugating enzyme vs. Ensembl Mouse
Match: Aasdh (aminoadipate-semialdehyde dehydrogenase [Source:MGI Symbol;Acc:MGI:2442517]) HSP 1 Score: 62.003 bits (149), Expect = 1.888e-9 Identity = 86/365 (23.56%), Postives = 150/365 (41.10%), Query Frame = 1
Query: 325 TYSMLNTKANIVAKNLIRSCNLEIPMNECLVGVLLDEGFERLYSIMACLKLGLVFVPLAKNRNSDLLKRIIDKCNLSIII-------------------------HKKCSDIDLLSNDNSIQTVLMDTLLDESKIIDSFNLLPREYNPPQSREKTV-------VLLHTSGSSGTPKTIKITSA------QLFNRLFWQWRNLPFKSNEIVCHRTGFMFVDNIVECL------SCILSTVTMVIVSPTEACDIVKLAEVIQKYSVSWILTVPSLLQKWLKQLDNYSDIXXXXXXXXXXXXXGEMLFPSLAKKCLATFNRNSCKLVNLYGSTEVCGDVCCQTLYSINDVRMNSKNKFLS---VGTPI 1278
+Y L + A+ ++ LI C+ + E +G+ G I+ L++ + P+ + L + KC+L ++ HK + L D + TVL D D+ K+ D + + E P+ + +LHTSG++GTPK +++ A Q F LF +I+ + F ++VE +C+L T V V P++ DI + ++ V+ + P+LL+++ +L S +L+ +SL + GE FPSL N ++ N+YG TEV T Y I + +NS K S +G+P+
Sbjct: 34 SYKALLSAASELSHFLIAHCDFG-GIRE--IGLYCQPGINLPSWILGILQVPAAYAPIDPDSPPSLSTYFMKKCDLKYVLVEKQQLSKFKSSHETVLNYDTVSVEHKDLALFRLHWEDGRVSTVLGDRA-DQHKVTDREDRVSAESRTPEKEHMDMRHDGCLAYVLHTSGTTGTPKIVRVPHACILPNIQHFRSLF------DITQEDILFLASPLTFDPSVVEIFVSLSSGACLLIVPTSVKVLPSKLADI-----LFSRHRVTVLQATPTLLRRFGSELIK-STVLSAHTSLRVLALGGEA-FPSLTILKSWRGKGNRTQIFNIYGITEVS---SWATFYRIPEEILNSAVKHESPVQLGSPL 378
BLAST of Ss-alanyl conjugating enzyme vs. UniProt/SwissProt
Match: sp|O68006|BACA_BACLI (Bacitracin synthase 1 OS=Bacillus licheniformis OX=1402 GN=bacA PE=3 SV=1) HSP 1 Score: 102.834 bits (255), Expect = 1.104e-20 Identity = 107/464 (23.06%), Postives = 202/464 (43.53%), Query Frame = 1
Query: 286 PNHIAAIHNDSFLTYSMLNTKANIVAKNLIRSCNLEIPMNECLVGVLLDEGFERLYSIMACLKLGLVFVPLAKNRNSDLLKRIIDKCNLSIIIHKKCSDIDLLSNDNSIQTVLMDTLLDESKIIDSFNLLPREYNPPQSREK-TVVLLHTSGSSGTPKTIKITSAQLFNRLFWQWRNLPFKSNEIVCHRTGFMFVDNIVECLSCILSTVTMVIVSPTEACDIVKLAEVIQKYSVSWILTVPSLLQKWLKQLDNYSDIXXX-XXXXXXXXXXGEMLFPSLAKKCLATFNRNSCKLVNLYGSTEVCGDVCCQTLYSINDVRMNSKNKFLSVGTPISNNQVFFESNSDN------EGEVIVIGKNVS--------------------PTCGLAFQTGDVG-FIADKKLFICGRIDDMVKVNGKKIFTKDISTAVMVHSDV-DNCYTIQLIISGRPQLVSFFTTKTEI 1587
P+H A + +TY LN KAN A+ L+R + + ++ D FE + I+ LK G ++P+ D + ++ ++++ D L I+TV + + D +LL E P +R T +++TSGS+GTPK + I R+ + N+++ + + F ++ + +L+ ++V++ + +LAEVI+K VS + +L + +DI L+ L ++ GE +K L R+ KL+++YG TE T Y IN++ + + + + +G+P++N V + GE+ + G +S P L ++TGD+ ++ D + GRID VK+ G +I +I + + +H D+ + T++ RP + ++ T EI
Sbjct: 4680 PDHTALVFGAQRMTYRELNEKANQTAR-LLREKGI---GRGSIAAIIADRSFEMIIGIIGILKAGGTYLPIDPETPRDRIDYMLKNSGAALLV-----TTDSLLKPFDIKTVDLCS--------DELHLLSEENLPRVNRSSDTAYIVYTSGSTGTPKGVVIPHYSA-ARVVKNTNYIDITGNDVILQLSNYSFDGSVFDIFGALLNGASLVLIEKETVLNTHELAEVIKKEQVSVMFITTAL-------FNTLADINIGCLAKLRKILFGGERASIPHVRKVLDHVGRD--KLIHVYGPTE---STVYATYYFINEI--DDEAETIPIGSPLANTSVLIMDEAGKLLPIGVPGELCIAGDGLSKGYLNREELTAEKFIPHPFIPGERL-YKTGDLAKWLPDGNIEFIGRIDHQVKIRGFRIELGEIESRLEMHEDINETIVTVREDEESRPYICAYITANREI 5110 HSP 2 Score: 91.2781 bits (225), Expect = 3.409e-17 Identity = 104/493 (21.10%), Postives = 201/493 (40.77%), Query Frame = 1
Query: 223 HCNNLATYFEMN-----CLNDCRKDNPNHIAAIHNDSFLTYSMLNTKANIVAKNLIRSCNLEIPMNECLVGVLLDEGFERLYSIMACLKLGLVFVPLAKNRNSDLLKRIIDKCNLSIII--HKKCSDIDLLSNDNSIQTVLMDTLLDE--SKIIDSFNLLPREYNPPQSREKTVVLLHTSGSSGTPKTIKITSAQLFNRLFWQWRNLPFKSNEIVCHRTGFMFVDNIVECLSCILSTVTMVIVSPTEACDIVKLAEVIQKYSVSWILTVPSLLQKWLK-QLDNYSDIXXXXXXXXXXXXXGEMLFPSLAKKCLATFNRNSCKLVNLYGSTEVCGDVCCQTLYSINDVRMNSKNKFLSVGTPISNNQVFFESNSDN------EGEVIVIGKNVS--------------------PTCGLAFQTGDVG-FIADKKLFICGRIDDMVKVNGKKIFTKDISTAVMVHSDV-DNCYTIQLIISGRPQLVSFFTTKTEI 1587
H NN T + N + P+H A + +TY LN KAN A+ L+R + + ++ D FE + I+ LK G ++P+ D + ++ ++++ K ID ++ + D E S + DS + T +++TSGS+GTPK + IT R+ + + ++ + + F ++ + +L+ ++V++ +I +L I + VS + +L +D S++ ++ GE +K L R+ KL+++YG TE T Y IN++ + + + + +G+P++N V + GE+ + G +S P L ++TGD+ ++ D + GRID VK+ G +I +I + + +H D+ + T++ RP + ++ T EI
Sbjct: 25 HFNNTKTDYPKNKTLHELFEEQAMKTPDHTALVFGAQRMTYRELNEKANQTAR-LLREKGI---GRGSIAAIIADRSFEMIIGIIGILKAGGAYLPIDPETPKDRIAFMLSDTKAAVLLTQGKAADGIDCEADIVQLDREASDGFSKEPLSSVNDSGD--------------TAYIIYTSGSTGTPKGV-ITPHYSVIRVVQNTNYIDITEDNVILQLSNYSFDGSVFDIFGALLNGASLVMIEKEALLNINRLGSAINEEKVSVMFITTALFNMIADIHVDCLSNL-------RKILFGGERASIPHVRKVLNHVGRD--KLIHVYGPTE---STVYATYYFINEI--DDEAETIPIGSPLANTSVLIMDEAGKLVPIGVPGELCIAGDGLSKGYLNREELTAEKFIPHPFIPGERL-YKTGDLAKWLPDGNIEFIGRIDHQVKIRGFRIELGEIESRLEMHEDINETIVTVREDEESRPYICAYITANREI 483 HSP 3 Score: 87.4261 bits (215), Expect = 5.863e-16 Identity = 110/469 (23.45%), Postives = 195/469 (41.58%), Query Frame = 1
Query: 286 PNHIAAIHNDSFLTYSMLNTKANIVAKNLIRSCNLEIPMNECLVGVLLDEGFERLYSIMACLKLGLVFVPLAKNRNSDLLKRIIDKCNLSIIIHKKCSDIDLLSN--DNSIQTVLMD----TLLDESKIIDSFNLLPREYNPPQSREKTVVLLHTSGSSGTPKTIKITSAQLFNRLFWQWRNLPFKSNEIVCHRTGFMFVDNIVECLSCILSTVTMVIVSPTEACDIVKLAEVIQKYSVSWILTVPSLLQKWLKQLDNYSDIXXXXXXXXXXXXXGEMLFPSLAKKCLATFNRNSCKLVNLYGSTEVCGDVCCQTLYSINDVRMNSKNKFLSVGTPISNNQVFFESNSD-------NEGEVIVIGKNVS--------------------PTCGLAFQTGDVG-FIADKKLFICGRIDDMVKVNGKKIFTKDISTAVMVHSDVDNCYTIQLIISGR-PQLVSFFTTKTEI 1587
P+H A + D LTY LN KAN VA+ L+R ++ + LVG++++ + + +I+ LK G ++P+ + ++ + + +II SD+ L SI+ + MD D S ID+ N QS + V ++TSGS+G PK + I L N W N+ F + E I + T+ +++ D+ KL + ++ I +P+ + + LDN+S L T+++ G+ L F S ++VN YG TE + T + I+ N + +G PI N +V+ N D GE+ + G+ ++ P + ++TGD+ + D + GR+D VK+ G +I +I ++ + ++ I P L ++ T K E+
Sbjct: 2139 PDHTAVVFEDQKLTYRQLNEKANQVAR-LLREKGVK---PDTLVGIMMERSSDMIAAILGVLKAGGAYLPIDPEYPPERMRYMAFDSEVKVII----SDVPLAEELTAESIELIHMDDERIAGQDRSD-IDNVN---------QSGDLAYV-IYTSGSTGKPKGVMIEHQSLINLCSWHQSCFEVGQNDNSSIYASISFDAFVWELFPYITAGATVHVLNQETRLDVEKLNRYFHDHHIT-ISFLPTPVCEQFTALDNHS--------LRTLLTGGDK---------LNVFKEKSYQIVNNYGPTE---NTVVATSFPIDKSHQN-----IPIGKPIDNVKVYI-LNKDLQLCPLGASGELCIAGEGLARGYVNRPELTREKFIGNPFVPGERM-YRTGDLAKMLPDGNIQFLGRVDQQVKIRGYRIEPGEIENRLLKYEKIEEAAVIAREDGDHDPYLCAYVTVKKEV 2560 HSP 4 Score: 83.9593 bits (206), Expect = 6.432e-15 Identity = 96/457 (21.01%), Postives = 187/457 (40.92%), Query Frame = 1
Query: 286 PNHIAAIHNDSFLTYSMLNTKANIVAKNLIRSCNLEIPMNECLVGVLLDEGFERLYSIMACLKLGLVFVPLAKNRNSDLLKRIIDKCNLSIIIHKKCSDIDLLSNDNSIQTVLMDTLLDESKIIDSFNLLPREYNPPQSREKTVVLLHTSGSSGTPKTIKITSAQLFNRLFWQ----WRNLPFKSNEIVCHRTGFMFVDNIVECLSCILSTVTMVIVSPTEACDIVKLAEVIQKYSVSWILTVPSLLQKWLKQLDNYSDIXXXXXXXXXXXXXGEMLFPSLAKKCLATFNRNSCKLVNLYGSTEVCGDVCCQTLYSINDVRMNSKNKFLSVGTPISNNQVFFESNSDNE---------GEVIVIGKNVS--------------------PTCGLAFQTGDVG-FIADKKLFICGRIDDMVKVNGKKIFTKDISTAVMVHSDVDNCYTIQLIISGRPQ 1554
P+H A D ++Y LN +AN +A L R + + + G+L + E + IM LK G ++P+ + + I+ ++S++ C+ D+ + ++ +D++ D L + P + +++TSGS+G PK + I + N + +++L N V ++F ++ + + +L T+ IV A D + L E K +++ P+ L + Y D L + ++ G+ L P + F SC + N+YG TE C D + S + S + +G P+ N ++ D E GE+ + G+ V+ P + ++TGD+ ++ D ++ GR D VK+ G +I ++ ++ H + +I+G+ Q
Sbjct: 3163 PDHPAVAFGDETISYRELNERANSLAFTL-RQKGV---GPDVIAGILTERSIEMIVGIMGILKAGGAYLPIDPAYPQERISYIVKDSDVSVL----CAAGDVDPGEAYTGDIIR---IDQTGQNDHVENLKHDIKP----QHLAYVIYTSGSTGKPKGVMIEHHSVNNLVHGLNERIYQHLDAHLN--VALVAPYIFDASVKQIFAALLFGHTLCIVPRETAWDAMSLIEYYSKNNINVSDMTPAHLN-----MLAYVDKTELEFDVKELIVGGDALTPDVIGGLFHKFPNLSCNITNVYGPTECCVDAASHQIESGKVPQTPS----IPIGRPLLNTSIYI---VDKELRPLPVGIAGELCIAGEGVARGYVNRPELTAEKFVDHPFEPGKKM-YKTGDLAMWLPDGQIEFLGRADHQVKIRGYRIELGEVEQQLLTHEKIKEA----AVIAGKDQ 3585 HSP 5 Score: 82.4185 bits (202), Expect = 1.618e-14 Identity = 122/598 (20.40%), Postives = 238/598 (39.80%), Query Frame = 1
Query: 277 KDNPNHIAAIHNDSFLTYSMLNTKANIVAKNLIRSCNLEIPMNECLVGVLLDEGFERLYSIMACLKLGLVFVPLAKNRNSDLLKRIIDKCNLSIIIHKKCSDIDLLSNDNSIQTVLMDTLLDESKIIDSFNLLPREYNPPQSREKTVVLLHTSGSSGTPKTIKITSAQLFNRLFWQWRNLPFKSNEIVCHRTGFMFVDNIVECLSCILSTVTMVIVSPTEACDIVKLAEVIQKYSVSWILTVPSLLQKWLKQLDNYSDIXXXXXXXXXXXXXGEMLFPSLAKKCLATFNRNSCKLVNLYGSTEVCGDVCCQTLYSINDVRMNSKNKFLSVGTPISNNQVFFESNSDN------EGEVIVIGKNVSP-----------------TCGLAFQTGDVGFI-ADKKLFICGRIDDMVKVNGKKIFTKDISTAVMVHSDVDNCYTIQLI-ISGRPQLVSFFTTKTEIGNKTKTKMEINNILMNHSLNVCLP-RLEYIKSIPIQPQSMKPDKKMLCKIAKKILSENRRMKTKLTHGKHXXXXXXXXXXXXXXXXXXNSQKHYIYEILAKHLSLLPDEIDDSMKFYDIGGDSLTIVICIADLNRK 1992
K P+ +A H+D +TY L+ K+N VA+ LI +E +GV+ + +++A LK G ++PL + + I K N I D + I+ F+ P + + ++ TSGS+G PK ++IT N + + + + F ++ + + S ++VI+ + D+ L E +K ++ +VP+++ D Y D L L ++ SG+ + L TF + ++++L G+TE G + Y I V + K+ + G P++N +++ + + EGE+ + G V+ G ++TGD G + D + GR D VK+ G ++ +I ++ H ++ I G L +F + I +++++ L + +P + I+ IP+ K DK+ L +A++ ++ + ++ N + + EI L D I+ + +Y+IGGDSL + I ++N++
Sbjct: 1099 KHGPDRMALKHHDEVMTYQELDEKSNQVARFLI-GKGVE---KGDYIGVIGKRSLGTIVNLLAVLKTGAAYIPLDPDYPEERKAYIQSKSNCKFFISHDVYD---------------------KEHIERFSKAPVDRK--VDLDDMAYVIFTSGSTGKPKGVQITHVPQRNTILDINEKFNVTEQDNIMGISSLCFDLSVYDVFGALSSGASLVIID--DQRDVFSLKETAEKERITIWNSVPAIMG---MTADVYPD-NELNHHLRLILLSGDWIPLQLPATIKKTF--KNAEVISLGGATE--GSIWS-IYYPIQKVEEDWKS--IPYGKPLANQKIYVLNQNKQLCPVGVEGELYIGGAGVASGYIHDQEKTEHSFIQHQELGYIYKTGDYGVLKEDGYVEFLGRKDSQVKIRGYRVEMGEIENTLVSHQEITKASVIDYTSPDGIKNLYAFVVAENAI-----SQLDVKEFLQKTLPDYMIPAKFVQIEEIPLTVNG-KVDKRTLHDLAEQHTADEGQRGGRM--------------------LPENETQAMLLEIWKDIFGL--DSINLDVSYYEIGGDSLKAISIITEINKR 1628
BLAST of Ss-alanyl conjugating enzyme vs. UniProt/SwissProt
Match: sp|P39581|DLTA_BACSU (D-alanine--D-alanyl carrier protein ligase OS=Bacillus subtilis (strain 168) OX=224308 GN=dltA PE=1 SV=1) HSP 1 Score: 98.9821 bits (245), Expect = 3.248e-20 Identity = 92/362 (25.41%), Postives = 166/362 (45.86%), Query Frame = 1
Query: 454 SIMACLKLGLVFVPLAKNRNSDLLKRIIDKCNLSIIIHKKCSDIDLLSNDNSIQTVLMDTLLDESKIIDSFNLLPREYNPPQSREKTVVLLHTSGSSGTPKTIKITSAQLFNRLFWQWRNLPFKSNEIVCHRTGFMFVDNIVECLSCILSTVTMVIVSPTEACDIVKLAEVIQKYSVSWILTVPSLLQKWLKQLDNYSDIXXXXXXXXXXXXXGEMLFPSLAKKCLATFNRNSCKLVNLYGSTEVCGDVCCQTLYSINDVRMNSKNKFLSVGTPISNNQVFFESNS----DNEGEVIVIGKNVS------PTC----------GLAFQTGDVGFIADKKLFICGRIDDMVKVNGKKIFTKDI 1479
S + +K G ++P+ + S+ + +II+ ++IH ID + IQTV + LL+ S + +E+ +T +++TSGS+G PK ++I++A L + W + P +I ++ F F ++++ C+ S T+ V+ L E ++K ++ + PS +Q L D+ L T + GE+L S+AK L F + K+ N YG TE V ++ NDV S++ + P N + E +GE+++ G +VS P A++TGD GFI D ++F GR+D +K++G ++ ++I
Sbjct: 68 SFLGSVKAGHPYIPVDLSIPSERIAKIIESSGAELLIHAAGLSIDAVGQQ--IQTVSAEELLENEGGSVSQDQWVKEH-------ETFYIIYTSGSTGNPKGVQISAANLQSFTDWICADFPVSGGKIFLNQAPFSFDLSVMDLYPCLQSGGTLHCVTKDAVNKPKVLFEELKKSGLNVWTSTPSFVQMCLMDPGFSQDL---LPHADTFMFCGEVLPVSVAKALLERFPK--AKIFNTYGPTEAT--VAVTSVEITNDVISRSESLPVGFAKPDMNIFIMDEEGQPLPEGEKGEIVIAGPSVSRGYLGEPELTEKAFFSHEGQWAYRTGDAGFIQDGQIFCQGRLDFQIKLHGYRMELEEI 413
BLAST of Ss-alanyl conjugating enzyme vs. UniProt/SwissProt
Match: sp|D4AU31|SWNK_ARTBC (Reducing polyketide synthase swnK OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=swnK PE=3 SV=1) HSP 1 Score: 100.908 bits (250), Expect = 3.827e-20 Identity = 120/457 (26.26%), Postives = 211/457 (46.17%), Query Frame = 1
Query: 286 PNHIAAIHNDSFLTYSMLNTKANIVAKNLIRSCNLEIPMNECLVGVLLDEGFERLYSIMACLKLGLVFVPLAKNRNSDLLKRIIDKCNLSIIIHKKCSDIDLLSNDNSIQTVLMDTLLDESKIIDSFNLLPREYNPPQSREKTVVLLHTSGSSGTPKTIKITSAQLFNRLFWQWRNLP-FKSNEIVCHRTGFMFVDNIVECLSCILSTVTMVIVSPTEACDIVKLAEVIQKYSVSWILTVPS----LLQKWLKQLDNYSDIXXXXXXXXXXXXXGEMLFPSLAKKCLATFNRNSCKLVNLYGSTEVCGDVCCQTLYSINDVRMNSKNKFLSVGTPISNNQVFFESNSDN------EGEVIVIGKNVSPTC------------------GLAFQTGDVG-FIADKKLFICGRIDDMVKVNGKKIFTKDISTAVMVHSDVDNCYTIQLIISGRPQLVSF 1566
P + A IH D+ +T+S LN ANI+A++LI+ L+ + LVG+ + + + ++A LKLG +VP+ ++ + +++ +I+ L S + +V D D S I+D+ NL + P +++TSGS+G PK ++I+ N L + P ++I+ T F + +E L ++S TMVI T + +L E+++++ V+ + P+ LL+ K S I+ GE L LA + LA + + N+YG +E T S+ V + VG P+ N +++ ++ + EGE+ + G +VS GL F+TGD+G F+A KL + GRID +VK+ G +I DI ++ H+ V + ++IS +LV++
Sbjct: 47 PKNTALIHRDTEITFSELNESANILARSLIKR-GLK---HGDLVGLAVSRSIDLIVVVLAVLKLGAAYVPIDPLFPAERINQMVSDAGPKLILLSGSPSKGLASWKDICISV--DEARDSS-IVDTTNL-EADIQP----HDLSYVIYTSGSTGKPKGVEISHGAAANFLSSLRKYEPGCNEHDILLAITTISFDMSALELLLPLVSGTTMVIADTTAVKNPRELLELMRRHHVTILQATPATWTMLLESGWKGDPRLSKIICG----------GEPLTRQLADRLLAAAD----SVWNVYGPSET-------TYGSVGRVGEGD----IVVGKPVVNGRIYVLDDNLSPAPVGCEGEIYIGGGSVSNGYRNNAELTRARFLANPFHGGLFFRTGDLGRFLAPGKLQVVGRIDGVVKIRGHRIDVGDIEAVLLDHASV----SAAVVISHDDRLVAY 462
BLAST of Ss-alanyl conjugating enzyme vs. UniProt/SwissProt
Match: sp|P94459|PPSD_BACSU (Plipastatin synthase subunit D OS=Bacillus subtilis (strain 168) OX=224308 GN=ppsD PE=1 SV=2) HSP 1 Score: 100.908 bits (250), Expect = 4.405e-20 Identity = 138/629 (21.94%), Postives = 252/629 (40.06%), Query Frame = 1
Query: 286 PNHIAAIHNDSFLTYSMLNTKANIVAKNL-IRSCNLEIPMNECLVGVLLDEGFERLYSIMACLKLGLVFVPLAKNRNSDLLKRIIDKCNLSIIIHKKCSDIDLLSN-DNSIQTVLMDTLLDESKIIDSFNLLPREYNPPQSREKTVVLLHTSGSSGTPKTIKITSAQLFNRLFWQWRNLPFKSNEIVCHRTGFMFVDNIVECLSCILSTVTMVIVSPTEACDIVKLAEVIQKYSVSWILTVPSLLQKWLKQLDNYSDIXXXXXXXXXXXXXGEMLFPSLAKKCLATFNRNSCKLVNLYGSTEVCGDVCCQTLYSINDVRMNSKNKFLSVGTPISNNQVFFESNSDNEGEVIVIGKNVSPTCGLA-------------------------FQTGDVG-FIADKKLFICGRIDDMVKVNGKKIFTKDISTAVMVHSDV-DNCYTIQLIISGRPQLVSFFTTKTEIGNKTKTKMEINN---ILMNHSLNVCLPRLEYIKSIPIQPQSMKPDKKMLCKIAKKILSENRRMKTKLTHGKHXXXXXXXXXXXXXXXXXXNSQKHYIYEILAKHLSLLPDEIDDSMKFYDIGGDSLTIVICIADLNRKGFSCTTEVFFHNHSIGELVECILEQSNS 2076
P A + D LTY L+ +N +A+ L R + P VG+++ F + SI+ K G +VP+ + + I+ +++ +D+ +L++ + I T+ ES D + LP+ S +++TSG++G PK + + + N L W+ F + + F F I + +LS V++ EA DI+ + + + ++ ++ VP L + L + + +L+ + E++ SLA C T +L N YG TE + +R K ++S+G PI QV +++ + V G+ GLA ++TGD ++AD L GRIDD VK+ G ++ TK+I + + V D + SG+ +L ++ TK + + E+ N + M+ + +E + S+P+ P K D+ L K E + +S+ I + K + L ++I + F+++GGDS+ + A L+R G + F + +I EL I + S
Sbjct: 2547 PKASALVSGDKTLTYQELDEWSNGIARALRSRGVKPDTP-----VGIMMHRSFSMIASILGVWKAGGCYVPIDPEYPKERKRYILSDSGTKLLMTINEADLGVLADFEGEILTI-------ESVEEDDKSPLPQM----SSAHHLAYIIYTSGTTGRPKGVMVEHKGIANTLQWRRNAYAFNETDTILQLFSFSFDGFITSMFTPLLSGAKAVLLHEEEAKDILAIKHQLSRQRITHMIIVPVLYRALLDVVQPEDVKTLRVVTLAGEAADRELIARSLA-ICPHT------ELANEYGPTE--------NSVATTVMRHMEKQAYVSIGQPIDGTQVLILNSNHQLQPIGVAGELCIAGTGLARGYVNLPELTERAFTQNPFKPEARMYRTGDAARWMADGTLEYLGRIDDQVKIRGYRVETKEIESVIRCIKGVKDAAVVAHVTASGQTELSAYVVTKPGLSTNA-VRSELQNKLPVFMHPAF------IEKLDSLPLSPNG-KLDRGALPKPVYNHEGERPFLPP-------------------------SSKMEQILADIWKEV-LGAEKIGTADSFFELGGDSIKALQVSARLHRIGKQMAVKDLFSHPTIQELAAYIRDSDTS 3110 HSP 2 Score: 71.633 bits (174), Expect = 3.399e-11 Identity = 109/500 (21.80%), Postives = 194/500 (38.80%), Query Frame = 1
Query: 205 ENDSYEHCNNLA-TYFEMN-----CLNDCRKDNPNHIAAIHNDSFLTYSMLNTKANIVAKNLI-RSCNLEIPMNECLVGVLLDEGFERLYSIMACLKLGLVFVPLAKNRNSDLLKRIIDKCNLSIIIHKKCS--------DIDLLSNDNSIQTVLMDTLLDESKIIDSFNLLPREYNP-----PQSREKTVVLLHTSGSSGTPKTIKITSAQLFNRLFWQWRNLPFKSNEIVCHRTGFMFVDNIVECLSCILSTVTMVIVSPTEACDIVKLAEVIQKYSVSWILTVPSLLQKWLKQLDNYSDIXXXXXXXXXXXXXGEMLFPSLAKKCLATFNRNSCKLVNLYGSTEVCGDVCCQTLYSINDVRMNSKNKFLSVGTPISNNQVFFESNSDNEGEVIVIGKNVSPTCGLA-------------------------FQTGDVG-FIADKKLFICGRIDDMVKVNGKKIFTKDISTAVMVHSDVDNCYTIQLIISGRPQLVSF 1566
E D E C+ T + MN L + P+ A I D +TY LN +AN +A LI R E V ++ E L I LK G ++P+ + + + +++ +I++ + +I L+ NS L R NP PQS +++TSGS+G PK ++I N L K ++++ H+T + F +I E + ++ ++ + +A+ I++ ++ + VPS+L +L+ + Y + + L V S GE L L + S + N YG TE + + D + K + + +G P+ + +++ + + V IG+ G+A ++TGDV ++ D + GR DD VK+ G +I +I A+ V SG P+L ++
Sbjct: 1475 ERDFIESCHLFEETGYSMNQTLHYALEQQAEKTPDQAAVIFEDGVMTYKELNEQANRIAWELIGRGVK-----PETTVAIIGKRSPEMLLGIYGILKAGGAYLPIDPDYPEERISFLLEDSGTNILLLQSAGLHVPEFTGEIVYLNQTNS-------------------GLAHRLSNPNVDVLPQS---LAYVIYTSGSTGMPKGVEIEHRSAVNFLNSLQSRYQLKHSDMIMHKTSYSFDASIWELFWWPYAGASVYLLPQGGEKEPEVIAKAIEEQKITAMHFVPSMLHAFLEHI-KYRSVPIKTNRLKRVFSGGEQLGTHLVSRFYELLPNVS--ITNSYGPTEATVEA------AFFDCPPHEKLERIPIGKPVHHVRLYLLNQNQRMLPVGCIGELYIAGAGVARGYLNRPALTEERFLEDPFYPGERMYKTGDVARWLPDGNVEFLGRTDDQVKIRGYRIEPGEIEAALRSIEGVREAAVTVRTDSGEPELCAY 1938
BLAST of Ss-alanyl conjugating enzyme vs. UniProt/SwissProt
Match: sp|F4K1G2|AEE19_ARATH (Putative acyl-activating enzyme 19 OS=Arabidopsis thaliana OX=3702 GN=At5g35930 PE=2 SV=1) HSP 1 Score: 99.7525 bits (247), Expect = 6.257e-20 Identity = 139/556 (25.00%), Postives = 232/556 (41.73%), Query Frame = 1
Query: 412 LVGVLLDEGFERLYSIMACLKLGLVFVPLAKNRNSDLLKRIIDKCNLSIIIHKKCSDID--LLSNDNSIQTVL--MDTLLDESKIIDSFNLLPREYNPPQSREKTVVLLHTSGSSGTPKTIKITSAQLFNRLFWQWRNLPFKSNEIVCHRTGFMFVDNIVECLSCILSTVTMVIVSPTEACD-IVKLAEVIQKYSVSWILTVPSLLQKWLKQLDNYSDIXXXXXXXXXXXXXGEMLFPSLAKKCLATFNRNSCKLVNLYGSTEVCGDVCCQTLYSINDVRMNSKNKFLSVGTPISNNQVFFESNSDN--EGEVIVIG--------------------------KNVSPTCG--LAFQTGDVGF-IADKKLFICGRIDDMVKVNGKKIFTKDISTAVMVHSDVDNCYTIQLIISGRPQLVS---FFTTKTEIGNKTKTKMEINNILMNHSLNVCLP-RLEYIKSIPIQPQSMKPDKKMLCKIAKKILSENRRMKTKLTHGKHXXXXXXXXXXXXXXXXXXNSQKHYIYEILAKHLSLLPDEIDDSMKFYDIGGDSLT 1959
+V + + E + S+ + L+ G F+PL + + + +I N+S++I S ++ L N +L MD L SF ++ + + K L++TSGS+G PK + T L NR W P + +T F+D+I E L ILS+ +VI T + ++ + + +++YS+S +L VPS+++ L L + L S L VV SGE FP L + +C +NLYGSTEV GD + + + + +G ISN +V + D EGE+ V G +++ CG L ++TGD G ++ L GR D VK+NGK++ ++I T + ++ D+ + L+ +L S F E + I N + V +P ++ +P+ S K D + L ++ M++ T NS I + + +LL E+ D F+ IGGDSL
Sbjct: 4 VVALYMPPSVEYVISVFSVLRCGEAFLPLDPSWPRERVLSLISSSNISLVIACGLSSVESHWLVERNVCPVLLFSMDEKLSVETGCSSFVWPCKK----ERQRKFCYLMYTSGSTGKPKGVCGTEQGLLNRFLWMQELYPVVGEQRFAFKTSVGFIDHIQEFLGAILSSTALVIPPFTLLKENMISIIDFLEEYSISRLLAVPSMIRAILPTLQHRGHNNKLQSCLKLVVLSGEP-FPVSLWDSLHSLLPETC-FLNLYGSTEVSGDCTYFDCSELPRLLKTEEIGSVPIGKSISNCKVVLLGDEDKPYEGEICVSGLCLSQGYMHSSIESEGYVKLHNNSLCNHLTNDCGSQLYYRTGDYGRQLSSGDLIFIGRRDRTVKLNGKRMALEEIETTLELNPDIAE--AVVLLSRDETELASLKAFVVLNKESNSSDGIIFSIRNWMGGKLPPVMIPNHFVLVEKLPLT-SSGKVDYEALARLKCPTTGAQDMMQSNGT----------------------NSLLQNIKKAVCD--ALLVKEVSDDDDFFAIGGDSLA 526
BLAST of Ss-alanyl conjugating enzyme vs. TrEMBL
Match: N1PB61 (Carrier domain-containing protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_210260 PE=4 SV=1) HSP 1 Score: 208.379 bits (529), Expect = 1.964e-52 Identity = 196/775 (25.29%), Postives = 356/775 (45.94%), Query Frame = 1
Query: 412 LVGVLLDEGFERLYSIMACLKLGLVFVPLAKNRNSDLLKRIIDKCNLSIIIHKKCSDIDLLSNDNSIQTVLMDTLLDESKIIDSFNLLPREYNPPQSREKTVVLLHTSGSSGTPKTIKITSAQLFNRLFWQWRNLPFKSNEIVCHRTGFMFVDNIVECLSCILSTVTMVIVSPTEACDIVKLAEVIQKYSVSWILTVPSLLQKWL--KQLDNYSDIXXXXXXXXXXXXXGEMLFPSLAKKCLATFNRNSCKLVNLYGSTEVCGDVCCQTLYSINDVRMNSKNKFLSVGTPISNNQVFFESNSDNE-----GEVIVIGKNVSPTCGLAFQTGDVGFIADKKLFICGRIDDMVKVNGKKIFTKDISTAVMVHSDVDNCYTIQLIISGRPQLVSFFTTKTEIGNKTKTKMEINNILMNHSLNV---CLPRLEYIKSIPIQPQSMKPDKKMLCKIAKKILSENRRMKTKLT-HGKHXXXXXXXXXXXXXXXXXXNSQKHYIYEILAKHLSLLPDEIDDSMKFYDIGGDSLTIVICIADLNRKGFSCTTEVFFHNHSIGELVECILEQSNSKIRNNSDYKLFKYDMKNDFTVEQIQYENKTEIIEFLVENFYTKELVVRYLNTPVKVFVEVVSEIFDLSLRSGCSFCIRNSISKSIVGLQLSEDSSYEIPNTSQLYKLDEENSLDCFLQNCKPRIAEFLKTPK---LNVSVVALSGTLNKSIVIQLLYFIEKHTIDLAQCKNYKTIETINTSEATKKICSELNYRLIKSTSMSHFLNESQ 2694
++G++ ER+ ++A +K+G FV L + +K +++ + ++ + + + I V LL ++K+ +P Y P ++ V +L+TSGS+G PK + + + + NR+ W W+ PF + C ++ +FVD++ E L+ +L + +++V + D I + V+ I+ VPS LQ L +D D L ++ V+SSGE L LAK+ L + +NS L N YGSTE GD+ S+ D+ S L++G P+ N + + + GEV V GKN+S L ++ GD+GFI D L+ CGR D +K+ G +I ++ V +++ LV+ + T+ KT M + IL N + +P L + ++P+Q + K DK+ML + K+++T HG + + I+A L++ +E+ + F+D+GG+SL V ++ LN + F + +SI E +E IL NN D ++ FT+ + + +++ E L E F T + + T F++ +S I L G SFCIR + ++ +V ++ D+S I ++ + E N + + + E LK ++ + A++ + + ++LL IE + LA+ K ++ I T+N++ T+ + + Y+L + F ESQ
Sbjct: 75 VLGLMFSASVERVAYMLAIIKIGSAFVSLDPSLPPPRMKAMLNASRVHFVLASRTMAEYVPETPDVITLV---DLLHDTKMSSG---IP--YVIP--KDPLVAVLYTSGSTGAPKAVHLKAMNIINRVVWSWKEYPFAREDRCCMKSSPLFVDSLTELLTPLLRGIPILMVDRRDVIDTQVFLRRIHQGKVTRIVVVPSQLQAMLTMNAIDTSIDF---LEFITMVISSGEELPVGLAKQFLQS-TKNSV-LCNFYGSTETTGDIASINFSSLEDIEKRSVGDHLALGKPVINTHITIKEPVETAFGVRVGEVCVSGKNIS-GAELEYRMGDLGFIQDNHLYYCGRNDKQIKIRGHRIDANEVEKVVY---QSHPAVKQVVVVGVDTDLVAIYETEA----KTYPGMLEDEILRNCGRKLPAYMMPVLFRVDAMPLQEHTGKLDKQMLKSL----------WKSRITCHGDNS-----------------------VAAIIANVLNISQEEMKMNRSFFDLGGNSLLAVTALSKLNDHDLKISFSDFINTNSIHE-IEQILSNKTEIYSNNFD--------RSAFTIHEFREDDEVEAFGLLAEEFTTNNPLDTAIGTTKSAFLKWMSSISQEMLADGLSFCIRETKTQRLVACCVNFDTSTVINPIAEHSAVLEIN------EGVEAPVRELLKEEGGRWMDSYLNAVTSSAAPDLKVRLLTLIEDEELRLAKEKGFRGIVTVNSNLVTQCLAEDYGYQLRNQIHVKSF--ESQ 776
BLAST of Ss-alanyl conjugating enzyme vs. TrEMBL
Match: A0A2H1VGR8 (SFRICE_007321 OS=Spodoptera frugiperda OX=7108 GN=SFRICE_007321 PE=4 SV=1) HSP 1 Score: 197.208 bits (500), Expect = 1.695e-48 Identity = 192/833 (23.05%), Postives = 380/833 (45.62%), Query Frame = 1
Query: 322 LTYSMLNTKANIVAKNLI-----RSCNLEIPMNECLVGVLLDEGFERLYSIMACLKLGLVFVPLAKNRNSDLLKRIIDKCNLSIIIHKKCSDIDLLSNDNSIQTVLMDTLLDESKIIDSFNLLPREYNPPQSREKTVVLLHTSGSSGTPKTIKITSAQLFNRLFWQWRNLPFKSNEIVC-HRTGFMFVDNIVECLSCILSTVTMVIVSPTEACDIVKLAEVIQKYSVSWILTVPSLLQKWLKQLDNYSDIXXXXXXXXXXXXXGEMLFPSLAKKCLATF-NRNSCKLVNLYGSTEVCGDVCCQTLYSINDVRMNSKNKFLSVGTPISNNQVFF---ESNSDNE---GEVIVIGKN--------------------VSPTCGLAFQTGDVGFIADKKLFICGRIDDMVKVNGKKIFTKDISTAVMVHSDVDNCYTI-QLIISGRPQLVSFFTTKTEIGNKTKTKMEINNILMNHSLNVCLPRLEYIKSIPIQPQSMKPDKKMLCKIAKKI-LSENRRMKTKLTHGKHXXXXXXXXXXXXXXXXXXNSQKHYIYEILAKHLSLLPDEIDDSMKFYDIGGDSLTIVICIADLNRKGFSCTTEVFFHNHSIGELVECILEQSNSKIRNNSDYKLFKYDMKNDFTVEQIQYENKTEIIEFLVENFYTKELVVRYLNTPVKV--FVEVVSEIFDLSLRSGCSFCIRNSISKSIVGLQLSEDSSYEIPNTSQLYKLDEENSL---DCFLQNCKPRIAEFLKTPK----LNVSVVALSGTLNKSIVIQLLYFIEKHTIDLAQCKNYKTIETINTSEATKKICSE-LNYRLIKSTSMSHFLN 2685
++Y+ L + N +A+ + + N + + ++ V + + +++A K G +VP+ + + I+ +++I+ + ++ + S + I V + L E+ + + + E P E ++L+TSGS+G PK ++++ + + NRL+WQ++ P+ +E+ C +T FVD++ E +L T+VI+S D KL V+ + ++ VP+LL+ L L + L L V SGE L LA + F + N KL N YGSTEV GDV T Y + + R + + +GTP++N V+ E N +E GEV V G+N V P G ++TGD G + + GR D +K+ G ++ +++ AV + VD C + + G P++++F T K E I L N + +P++ ++SIP+ K D++ L K+ + +++ + +L + S + + E+L + I FY++GG+SL + I L KG+ F ++GE++ + S S +++ + +L F+ E + E+K E+IE +V +FY K + ++L + + ++S+ + +++ S ++NS S + + L+ D+ E P+ DE L FL+ + + + + P L+ ++A + +L + + +E T+++A+ + + + T NTS T+++ ++ L Y+ + ++ +++
Sbjct: 48 VSYAELEARTNALARAITSRAAPKGANRD---GDYVIAVCMQPTHNTITTLLATWKAGAAYVPMEPSFPQARISHILKDAEPALVIYDETANPAMFSG-SGIPAVSFEELSHEASGLSADKVKDMEALSPARSESIAIVLYTSGSTGIPKGVRLSYSAVCNRLWWQFQTFPYTDSEVHCVWKTALTFVDSVCEIWGPLLHGRTLVILSRETTRDPQKLVNVLADNQIQRLVLVPTLLRSILMYL-SLKPSERPLKHLKLWVCSGETLSKDLAAQFFKNFGDHNGYKLANFYGSTEVMGDV---TYYVLENARQLDIHPTIPIGTPLNNCAVYLLDEEMNPAHESEPGEVWVAGRNLAEGYVGGQGADKFIDNPHAVHPDFGRLYRTGDFGVLQKGVILYAGRTDSQIKIRGHRVDLQEVDRAVAGVNGVDKCVVLCYGLERGNPEILAFVTIKPE---ARIAAQHIEAALKNSLTHYMIPQVIEVESIPLLVNG-KVDRQALLKMYENTNNNDDSEIPLELDYSSVSPSDMQAAKVLFET----------VGEVLGRAAR---GAISVRAGFYELGGNSLNSIYTITRLRDKGYYIDISDFLGASNLGEVLSHM---STSPQQDDGEKEL--------FSAEPLADEHKQEVIEMIVSSFYNKAELEQFLKHEIDTNDYAVLISDTWPALIKAQLSVVLKNS-SGVPIAVALNFDARDE-PDVES----DETGGLMKIMIFLEFVESSVRDAMLPPGKGTILHSFMMATAESLTPRENVAAIRALETATMNIAKERGFHGVFTTNTSPLTQQLGTDVLGYQTLLDYQINQYVD 838
BLAST of Ss-alanyl conjugating enzyme vs. TrEMBL
Match: A0A2A4J0M6 (Uncharacterized protein (Fragment) OS=Heliothis virescens OX=7102 GN=B5V51_8779 PE=4 SV=1) HSP 1 Score: 195.282 bits (495), Expect = 4.979e-48 Identity = 191/830 (23.01%), Postives = 368/830 (44.34%), Query Frame = 1
Query: 322 LTYSMLNTKANIVAKNLIRSCNLEIPMNEC--LVGVLLDEGFERLYSIMACLKLGLVFVPLAKNRNSDLLKRIIDKCNLSIIIHKKCSDIDLLSNDNSIQTVLMDTLLDESKIIDSFNLLPREYNPPQSREKTVVLLHTSGSSGTPKTIKITSAQLFNRLFWQWRNLPFKSNEIVC-HRTGFMFVDNIVECLSCILSTVTMVIVSPTEACDIVKLAEVIQKYSVSWILTVPSLLQKWLKQLDNYSDIXXXXXXXXXXXXXGEMLFPSLAKKCLATF-NRNSCKLVNLYGSTEVCGDVCCQTLYSINDVRMNSKNKFLSVGTPISNNQVFF------ESNSDNEGEVIVIGKNVS--------------------PTCGLAFQTGDVGFIADKKLFICGRIDDMVKVNGKKIFTKDISTAVMVHSDVDNCYTI-QLIISGRPQLVSFFTTKTEIGNKTKTKMEINNILMNHSLNVCLPRLEYIKSIPIQPQSMKPDKKMLCKIAKKILSENRRMKTKLTHGKHXXXXXXXXXXXXXXXXXXNSQKHYIYEILAKHLSLLPDEIDDSMKFYDIGGDSLTIVICIADLNRKGFSCTTEVFFHNHSIGELVECILEQSNSKIRNNSDYKLFKYDMKNDFTVEQIQYENKTEIIEFLVENFYTKELVVRYLNTPVKV--FVEVVSEIFDLSLRSGCSFCIRNSISKSIVGLQLSEDS----SYEIPNTSQLYKLDEENSLDCFLQNCKPRIAEFLKTPK----LNVSVVALSGTLNKSIVIQLLYFIEKHTIDLAQCKNYKTIETINTSEATKKICSE-LNYRLIKSTSMSHFLN 2685
++Y+ L + N +A+ + P + +V V + + +++A K G +VP+ + I+ S++I+ ++ + S + + +V + L E+ + + L E E ++L+TSGS+G PK ++++ + + NRL+WQ+R P+ +E+ C +T FVD++ E +L T++I+S D KL V+ + + ++ VP+LL+ L L + L L V SGE L LA + F + N KL N YGSTEV GDV L + + + M+ + +G+P+ N V+ + GEV V G N++ P G ++TGD G + + GR D VK+ G ++ +++ AV VD C + + G P+++SF T + + I L N + +P++ + SIP+ K D++ L K + EN T + ++ + + E+L + I FY++GG+SL + I L KG+ F ++GE++ + S S +++D +L FT E + E+K ++IE +V +FY K + ++L + + ++S+ + + + S ++NS S + V + L+ D+ E T L K + FL+ + + + + P L+ ++A + +L+ + + +E T+ LAQ + ++ + T NTS T+++ ++ L Y+ + ++ +++
Sbjct: 7 ISYAELWARTNALARAITARAQPSGPNRDGDFVVAVCMQPTHNTITTLLATWKAGAAYVPMEPTFPQARISHILKDAEPSLVIYDDTANPAMFSG-SGVPSVSYEELAHEASGLSADELQDGEVLAVARSESIGIVLYTSGSTGIPKGVRLSYSAVCNRLWWQFRTFPYSDSEVNCVWKTALTFVDSVCEIWGPLLHGRTLIILSRETTRDPQKLVNVLAENQIQRLVLVPTLLRSILMYL-SLKPTERPLQHLKLWVCSGETLSKDLAAQFFKNFGDHNGYKLANFYGSTEVMGDVTYYVLENTSQLDMHPT---IPIGSPLDNCAVYLLDEEMNPARQSEPGEVWVAGANLAAGYVGGQGADKFLDNPHAAHPDFGRLYRTGDFGVLEKGVILFAGRTDSQVKIRGHRVDLQEVDRAVAAVDGVDKCVVLCYGLERGNPEILSFVTIRP---DARVAAQHIEAALKNSLTHYMIPQVIEVDSIPLLVNG-KVDRQALLK-----MYEN----TNNNDDSEIPLDIDYTSVSQSELVAARVLFETVGEVLGRAAR---GAISLKAGFYELGGNSLNSIYTITRLRDKGYYIDISDFLGASNLGEVLSHM---STSPHSDDADNEL--------FTAEPLADEHKQQVIEMIVSSFYNKAELEQFLKHEIDTNDYAVLISDTWPALISAKLSVVLKNS-SGTAVAVALNFDARDEPDVESEETGGLMK------IMIFLEFVESSVRDTMLPPGKGTILHSFMMATAESLSPRENVAAIRALEHATMTLAQEQGFRGVFTTNTSPLTQQLGTDVLGYKTLLDYQINQYVD 797
BLAST of Ss-alanyl conjugating enzyme vs. TrEMBL
Match: A0A0R3SIC4 (Carrier domain-containing protein OS=Hymenolepis diminuta OX=6216 GN=HDID_LOCUS4687 PE=4 SV=1) HSP 1 Score: 196.438 bits (498), Expect = 5.780e-48 Identity = 234/843 (27.76%), Postives = 363/843 (43.06%), Query Frame = 1
Query: 415 VGVLLDEGFERLYSIMACLKLGLVFVPLAKNRNSDLLKRIIDKCNLSIIIHKKCSDIDLLSNDNSIQ-------TVLMDTLLDESKIIDSFNLLPREYNPPQSREKTVVLL-HTSGS-SGTPKTIKITSAQLFNRLFWQWRN-LPFKS-NEIVCHRTGFMFVDNIVECLSCILSTVTMVIVSPTE------ACDIVKLAEVIQKYSVSWILTVPSLLQKWLKQLDNYSDIXXXXXXXXXXXXXGEMLFPSLAKKCLATFNRNSCKLVNLYGSTEVCGDVCCQTLYSINDV-----RMNSKNKFLSVGTPISNNQVFFESNSDN----------EGEVIVIGKNV-------------------------SPTCGLAFQTGDVGFIADKK--LFICGRIDDMVKVNGKKIFTKDISTAVMVHSDVDNC--------YTIQLIIS---GRPQLVSFFTTKTEIGNKTKTKMEINNILMNHSLNVCLPRLEYIKSIPIQPQSMKPDKKMLCKIAKKILSENRRMKTKLTHGKHXXXXXXXXXXXXXXXXXXNSQKHYIYEILAKHLSLLP-----DEIDDSMKFYDIGGDSLTIVICIADLNRKGFSCTTEVFFHNHSIGELVECILEQSN---------SK---IRNNSDYKLFKYDMKNDFT----VEQIQYENKTEIIEFLVENFYTKELVVRYLNTPVKVFVEVVSEI-----FDLSLRSGCSFCIRNSISKSIVGLQLSEDSSYEIPNTSQ-LYKLDEENSLDCFLQNCKPRIAEFLKTPKLNVSVVALSG-TLNKS-IVIQLLYFIEKHTIDLAQCKNYKTIETINTSEATKKICSELNY 2646
V L+ G R+Y +AC+ L L ++P+ +S+L K D+ NL I I +++D +++ T +D LL ++ D +P E + E ++LL TSGS S PK + + ++QL NRL WQW P +T +FVD+ E +L +++ +E DI L +I +Y ++ I +VP+ L+ W+ Q+ SL +VSSG+ML +LA K A F + +L+NLYGSTEV GDV + S +V ++++ + L VG PISN +++ EGEV+ G + SP FQTGD+GF+ K L+I GR+DD+VKVNG KI ++ + + +D TI L + QLV F+ T GNKT T ++ I+ H ++ + + PI S K DK L + K E R + T T + + ++ I I A++L+L E DD+ F+ IGGDS+ + I++L +KG+ +F N IG+L++ + +N SK IR K F D D + I ++ E + +E V + +K F EV+ + F LS +G + +S +G L + E P S LY+ EE + C R AE L+ P S L LN +IQLL IE+ I+ A+ + I +INTS T+ + EL Y
Sbjct: 111 VATYLNAGINRVYIQLACMYLRLTYIPM----DSNLPK---DRINL---IFNSFEPIAFITDDTNVKIEDIETLTFTVDELLKKANAAD----IPAEEPLFKGMENPLILLLFTSGSTSPIPKGVPLRNSQLANRLSWQWSTESPLAGITGPSLIKTSCLFVDSFTEMFGPLLGGRPIIVSGTSEITSERLVTDIEILKRLISQYQITQITSVPTQLELWMNQMAGDG---TAFKSLRVIVSSGQMLPYTLALKIFAVFGSGNLRLLNLYGSTEVAGDVTAISFDSKEEVSKAAIQLSTGSLVLPVGKPISNTEIYVVEGGTGSSSKVAPMGLEGEVLTSGAGILIEEPILTLSDKKLAPTLSRNYLMKSPCFPRLFQTGDIGFVCSKSENLYITGRVDDVVKVNGVKISVGNVD---QILAGLDRSSGKFASLGATITLALEKGETSSQLVCFYQTS---GNKTFTDRDLAAIVKTHLMSFLHVIFVEVPTFPILKHSGKIDKVKLGQNYKTGAYEKRSIVTSATKRE------------------TSDEREVIRRIFARNLALSEIAVENGEPDDNADFFLIGGDSILAAVVISELRKKGYKVNMNLFTENQKIGDLLDALTNVANPIAQSSLNGSKVPFIREFQPGKTFASDSALDAQWRNLIANILADSFVEFEPMAIAINLNRETVYQIAIGRMKQF-EVMPGLLFVAGFTLSDPNGPLNDLFPEVSAVTLGYPL---LTPESPGVSDPLYQKIEEA-----FEICNSR-AEKLEDPSKCFSSAMLGARRLNAGNDIIQLLILIEESLINGAKRLGFNAIRSINTSVVTRIMSDELGY 902
BLAST of Ss-alanyl conjugating enzyme vs. TrEMBL
Match: A0A564ZE13 (Carrier domain-containing protein (Fragment) OS=Hymenolepis diminuta OX=6216 GN=WMSIL1_LOCUS14546 PE=4 SV=1) HSP 1 Score: 195.667 bits (496), Expect = 7.729e-48 Identity = 234/843 (27.76%), Postives = 363/843 (43.06%), Query Frame = 1
Query: 415 VGVLLDEGFERLYSIMACLKLGLVFVPLAKNRNSDLLKRIIDKCNLSIIIHKKCSDIDLLSNDNSIQ-------TVLMDTLLDESKIIDSFNLLPREYNPPQSREKTVVLL-HTSGS-SGTPKTIKITSAQLFNRLFWQWRN-LPFKS-NEIVCHRTGFMFVDNIVECLSCILSTVTMVIVSPTE------ACDIVKLAEVIQKYSVSWILTVPSLLQKWLKQLDNYSDIXXXXXXXXXXXXXGEMLFPSLAKKCLATFNRNSCKLVNLYGSTEVCGDVCCQTLYSINDV-----RMNSKNKFLSVGTPISNNQVFFESNSDN----------EGEVIVIGKNV-------------------------SPTCGLAFQTGDVGFIADKK--LFICGRIDDMVKVNGKKIFTKDISTAVMVHSDVDNC--------YTIQLIIS---GRPQLVSFFTTKTEIGNKTKTKMEINNILMNHSLNVCLPRLEYIKSIPIQPQSMKPDKKMLCKIAKKILSENRRMKTKLTHGKHXXXXXXXXXXXXXXXXXXNSQKHYIYEILAKHLSLLP-----DEIDDSMKFYDIGGDSLTIVICIADLNRKGFSCTTEVFFHNHSIGELVECILEQSN---------SK---IRNNSDYKLFKYDMKNDFT----VEQIQYENKTEIIEFLVENFYTKELVVRYLNTPVKVFVEVVSEI-----FDLSLRSGCSFCIRNSISKSIVGLQLSEDSSYEIPNTSQ-LYKLDEENSLDCFLQNCKPRIAEFLKTPKLNVSVVALSG-TLNKS-IVIQLLYFIEKHTIDLAQCKNYKTIETINTSEATKKICSELNY 2646
V L+ G R+Y +AC+ L L ++P+ +S+L K D+ NL I I +++D +++ T +D LL ++ D +P E + E ++LL TSGS S PK + + ++QL NRL WQW P +T +FVD+ E +L +++ +E DI L +I +Y ++ I +VP+ L+ W+ Q+ SL +VSSG+ML +LA K A F + +L+NLYGSTEV GDV + S +V ++++ + L VG PISN +++ EGEV+ G + SP FQTGD+GF+ K L+I GR+DD+VKVNG KI ++ + + +D TI L + QLV F+ T GNKT T ++ I+ H ++ + + PI S K DK L + K E R + T T + + ++ I I A++L+L E DD+ F+ IGGDS+ + I++L +KG+ +F N IG+L++ + +N SK IR K F D D + I ++ E + +E V + +K F EV+ + F LS +G + +S +G L + E P S LY+ EE + C R AE L+ P S L LN +IQLL IE+ I+ A+ + I +INTS T+ + EL Y
Sbjct: 106 VATYLNAGINRVYIQLACMYLRLTYIPM----DSNLPK---DRINL---IFNSFEPIAFITDDTNVKIEDIETLTFTVDELLKKANAAD----IPAEEPLFKGMENPLILLLFTSGSTSPIPKGVPLRNSQLANRLSWQWSTESPLAGITGPSLIKTSCLFVDSFTEMFGPLLGGRPIIVSGTSEITSERLVTDIEILKRLISQYQITQITSVPTQLELWMNQMAGDG---TAFKSLRVIVSSGQMLPYTLALKIFAVFGSGNLRLLNLYGSTEVAGDVTAISFDSKEEVSKAAIQLSTGSLVLPVGKPISNTEIYVVEGGTGSSSKVAPMGLEGEVLTSGAGILIEEPILTLSDKKLAPTLSRNYLMKSPCFPRLFQTGDIGFVCSKSENLYITGRVDDVVKVNGVKISVGNVD---QILAGLDRSSGKFASLGATITLALEKGETSSQLVCFYQTS---GNKTFTDRDLAAIVKTHLMSFLHVIFVEVPTFPILKHSGKIDKVKLGQNYKTGAYEKRSIVTSATKRE------------------TSDEREVIRRIFARNLALSEIAVENGEPDDNADFFLIGGDSILAAVVISELRKKGYKVNMNLFTENQKIGDLLDALTNVANPIAQSSLNGSKVPFIREFQPGKTFASDSALDAQWRNLIANILADSFVEFEPMAIAINLNRETVYQIAIGRMKQF-EVMPGLLFVAGFTLSDPNGPLNDLFPEVSAVTLGYPL---LTPESPGVSDPLYQKIEEA-----FEICNSR-AEKLEDPSKCFSSAMLGARRLNAGNDIIQLLILIEESLINGAKRLGFNAIRSINTSVVTRIMSDELGY 897
BLAST of Ss-alanyl conjugating enzyme vs. Ensembl Cavefish
Match: aasdh (aminoadipate-semialdehyde dehydrogenase [Source:NCBI gene;Acc:103042755]) HSP 1 Score: 61.2326 bits (147), Expect = 5.281e-9 Identity = 101/477 (21.17%), Postives = 187/477 (39.20%), Query Frame = 1
Query: 262 LNDCRKDNPNHIAAIHN-DSF---LTYSMLNTKANIVAKNLIRSCNLEIPMN-ECLVGVLLDEGFERLYSIMACLKLGLVFVPLAKNRNSDLLKRIIDKCNL------------------SIIIHKKCSDID-------------LLSNDNSIQTVLMDTLLDESKIIDSFNLLPREYNPPQSREKTVV--------LLHTSGSSGTPKTIKITSAQLFNRLFWQWRNLPFKSNEIVCHRTGFMFVDNIVECLSCILSTVTMVIVSPTEACDIVKLAEVIQKYSVSWIL-TVPSLLQKWLKQLDNYSDIXXXXXXXXXXXXXGEMLFPSLAKKCLATFNRNSCKLVNLYGSTEVCGDVCCQTLYSINDVRMNSKNKFLSVGTPISNNQVFFESN-----SDNEGEVIVIGKN--------VSPTCGLAFQTGDVGFIADKKLFICGRIDDMVKVNGKKIFTKDISTAVMVHSDVDNC 1518
L R+ N A +H+ DS LTY L T V++ L ++ + + E +G+ I+ L+L +VPL L RI++KC+L ++ K C+ ++S+ S +T D + + K P E P + T V +LHTSG++G PK +K+ + + ++V + F ++VE + S +++V +LA V+ K + S +L P+L++ + ++ +++ S V++ G PSLA RN ++ NLYG+TEV CC L + N + + +G P+ + + ++ EG+ + G++ ++ G TGD + + + GR D +VK +G+++ + VD+C
Sbjct: 10 LQAARRHAGNTAAVLHSGDSARTCLTYRELIT----VSQQLTEQLHISVGEHREQSIGLFCTAHIHLPAWIIGVLQLPAAYVPLDPVSPPLLTLRIMEKCSLRFCLIQNSLLQQFQGAFSGLLSLKVCTTFSTSNLTLVKMQRNSVISSHPSSETQPADDQIWQKK--------PEEKQHPSAAVPTAVNQEEQLAYILHTSGTTGLPKIVKVPHKCIVPNILHLRTLFQMTPEDVVFLASPLTFDPSVVEMFLALTSGACLLMVPSAVKRMPGRLARVLFKENKSTVLQATPALVRSFGGRV--LREVVLSADSSLRVLALGGEACPSLAVLRSWKQERNKTRIFNLYGTTEVSCWACCYELPNNLLSPDNMDDGSVPLGGPLMDTALEVRDKGGGLVTEGEGQAFIGGQDRVCLLDDEITAARGTMRATGDWVMVKNSHTYYLGRKDRLVKRHGQRVNLDALQQIAESLPQVDSC 472
BLAST of Ss-alanyl conjugating enzyme vs. Ensembl Sea Lamprey
Match: aasdh (aminoadipate-semialdehyde dehydrogenase [Source:NCBI gene;Acc:570338]) HSP 1 Score: 60.4622 bits (145), Expect = 1.502e-9 Identity = 77/345 (22.32%), Postives = 145/345 (42.03%), Query Frame = 1
Query: 721 LLHTSGSSGTPKTIKITSAQLFNRLFWQWRNLPFKSNEIVCHRTGFMFVDNIVECLSCILSTVTMVIVSPTEACDIVKLAEVIQKYSVSWIL-TVPSLLQKWLKQLDNYSDIXXXXXXXXXXXXXGEMLFPSLAKKCLATFNR--NSCKLVNLYGSTEVCGDVCCQTLYSINDVRM--NSKNKFLSVGTPISNNQVFFESNSD-----NEGEVIVIGKNVSPTC----------GLAFQTGDVGFIADKKLFICGRIDDMVKVNGKKIFTKDISTAVMVHSDVDNCYTIQLIISGRPQLVSFFTTKTEIGNKTKTKMEINNILMNHSLNVCLPRLEYIKSIPIQP 1695
+LHTSG++G PK + + A + + ++V + F ++VE + S +++V P L V+ + S + +L P+LL+++ + D++ S V++ G FP L+ L T+ N +L NLYG TEV C T + I + + + VG P+ +V + +G++ + G+ TC G TGDVG +A + ++ GR D +VK +G+++ + + V+ C + + G+ L+ F + E+ ++L +H+ R + + IP P
Sbjct: 4 VLHTSGTTGPPKIVHVPHACIVPNITHLRSLFAMSPEDVVVLVSALTFDPSVVELFVALASGAMLLLVPPYVRRSPRDLQRVLFQRSRATVLQATPTLLRRFGAAM--LRDVVLACGSPLRVLALGGEPFPPLS--VLRTWKSMGNRTRLFNLYGITEVS---CWATCHEIPTQHLLPGCEQCAVPVGKPLLGTRVEVRDGAGLVLHHGQGQIYIGGERR--TCLLDGEAELVPGTMRATGDVGRVAGRLVYYVGRSDGLVKRHGQQLDPLLLQQTAECLAAVETCAAV--MTPGQ-TLLLLFVVPAMDALRGGIMQELASLLPSHA------RPDSVMLIPTLP 330
BLAST of Ss-alanyl conjugating enzyme vs. Ensembl Sea Lamprey
Match: aasdh (aminoadipate-semialdehyde dehydrogenase [Source:NCBI gene;Acc:570338]) HSP 1 Score: 60.077 bits (144), Expect = 2.247e-9 Identity = 110/536 (20.52%), Postives = 206/536 (38.43%), Query Frame = 1
Query: 721 LLHTSGSSGTPKTIKITSAQLFNRLFWQWRNLPFKSNEIVCHRTGFMFVDNIVECLSCILSTVTMVIVSPTEACDIVKLAEVI-QKYSVSWILTVPSLLQKWLKQLDNYSDIXXXXXXXXXXXXXGEMLFPSLAKKCLATFNR--NSCKLVNLYGSTEVCGDVCCQTLYSINDVRM--NSKNKFLSVGTPISNNQVFFESNSD-----NEGEVIVIGKNVSPTC----------GLAFQTGDVGFIADKKLFICGRIDDMVKVNGKKIFTKDISTAVMVHSDVDNCYTIQLIISGRPQLVSFFTTKTEIGNKTKTKMEINNILMNHSLNVCLPRLEYIKSIPIQPQSMKPDKKMLCKIAKKILSENRRMKTKLTHGKHXXXXXXXXXXXXXXXXXXNSQKHYIYEILAKHLSLL-----PDEI---------------------DDSMKFYDIGGDSLTIVICIADLNRK-GFSCT--TEVFFHNHSIGELVECILEQSNSKIRNNS--DYKLFKYDMKNDFTVEQIQYENKTEII 2175
+LHTSG++G PK + + A + + ++V + F ++VE + S +++V P L V+ Q+ + + P+LL+++ + D++ S V++ G FP L+ L T+ N +L NLYG TEV C T + I + + + VG P+ +V + +G++ + G+ TC G TGDVG +A + ++ GR D +VK +G+++ + + V+ C + + G+ L+ F + + + E+ ++L +H+ +PD ML I L+ +HGK +++ +YE+ H+S + PD++ D + F +GGDSL +V D+ R+ G S ++ S + E +N+ N+ K+ K ++ D EQ E K ++
Sbjct: 4 VLHTSGTTGPPKIVHVPHACIVPNITHLRSLFAMSPEDVVVLVSALTFDPSVVELFVALASGAMLLLVPPYVRRSPRDLQRVLFQRSRATVLQATPTLLRRFGAAM--LRDVVLACGSPLRVLALGGEPFPPLS--VLRTWKSMGNRTRLFNLYGITEV---SCWATCHEIPTQHLLPGCEQCAVPVGKPLLGTRVEVRDGAGLVLHHGQGQIYIGGERR--TCLLDGEAELVPGTMRATGDVGRVAGRLVYYVGRSDGLVKRHGQQLDPLLLQQTAECLAAVETCAAV--MTPGQTLLLLFVVPASCNVSLAVEREELASLLPSHA---------------------RPDSVML--IPTLPLT---------SHGK-----------------VSSAELLALYEVENAHISRVGKPWEPDQLIRTLWACWQEALGRGVSAEAADLAASFLRVGGDSLGVVRLSGDVERRLGASLPGLPDLLLQYSSSSGIAENTGHSTNTNSENSGKEPRKILKRKVEGDIP-EQEAPEGKRTVM 478
BLAST of Ss-alanyl conjugating enzyme vs. Ensembl Nematostella
Match: EDO29940 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T272]) HSP 1 Score: 93.5893 bits (231), Expect = 1.263e-19 Identity = 139/550 (25.27%), Postives = 227/550 (41.27%), Query Frame = 1
Query: 217 YEHCNNLATYFEMNCLNDCRKDNPNHIAAIHNDS-FLTYSMLNTKANIVAKNLI-RSCNLEIPMNECLVGVLLDEGFERLYSIMACLKLGLVFVPLAKNRNSDLLKRIIDKCNLSIIIHKKCSDIDLLSNDNSIQTVLMDTLLDESKIIDSFNLLPREYNPP--QSREKTVVLLHTSGSSGTPKTIKITSAQLFNRLFWQWRNLPFKSNEIVCHRTGFMFVDNIVECLSCILSTVTMVIVSPTEACDIVKLAEVIQKYSVSWILTVPSLLQKWLKQLDNY--SDIXXXXXXXXXXXXXGEMLFPSLAKKCLATFNRNSCKLVNLYGSTEVCGDVCCQTLYSIN-----DVRMNSKNKFLSVGTPISNNQVFFESNSDN------EGEVIVIGKNVS-----------------PTCGLA------FQTGDVGF-IADKKLFICGRIDDMVKVNGKKIFTKDISTAVMVHSDVDNCYTIQLIISGRPQ-LVSFF-----TTKTEIGNKTKTKMEINNILMNHSLNVCLPRLEYIKSIPIQPQSMKPDKKML 1725
YE L F L P A + +D L +S LN +I+A NL + C + +VG+ ++ E + ++ LK G ++PL + +LLK I + + II L S S+ +++ DE ++ L Y PP S + ++++SG++G PK I W++ N P+K E V F++ E L +L + + ++ D L +QK+ ++ IL PSL + L NY DI + LSSL V GE++ +L + + + KL+NLY +E C DV L ++ + +K KF VG + Q+ GE+ V G ++ P A + TGD G+ ++ L ICGR D MVK+ G I + + A++ ++ C + L G + LV++ TK +I KT++ I + L ++SIP+ P S K DKK L
Sbjct: 3 YEKYGCLHEIFTRQALK-----TPEKTAVVCDDGRHLNFSELNRLTDILASNLRHKGCG-----KDKVVGIYMERCLEYPIAYISALKAGGAYMPLELSYPENLLKSIFEDSSPVAIITTDELKSRLPS---SVPVLVLSKGWDERLQKENKEL---GYLPPVESSLDDLAYVVYSSGTTGKPKGIACPHRGAVFSYTWRFTNYPYKDGERVACNVFFVW-----EMLRSLLVGIPLYVIPDDIIYDPPLLLRFLQKHKITRILFTPSLFEAIL----NYEEEDINSYLSSLQVVWLCGEVVTSALRDRAMRAIPQ--IKLLNLYSISE-CHDVAVADLTNMKFQEELPEKDENKRKFCPVGKLLPGVQIVIMDKKMKPLPVGLPGEIFVGGPTLARGYLNRPELNKERFISRPASVPASVGERLYSTGDWGYMLSSGALEICGRCDSMVKIRGYSIEIQAVEAALLQLPMINACVVLALGEEGTDKYLVAYVVPEGKVTKKDIRATLKTRLPFYMIPAHFVL---------LQSIPVAP-SGKLDKKRL 514
BLAST of Ss-alanyl conjugating enzyme vs. Ensembl Nematostella
Match: EDO25952 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7TDA1]) HSP 1 Score: 76.2554 bits (186), Expect = 3.708e-15 Identity = 71/283 (25.09%), Postives = 120/283 (42.40%), Query Frame = 1
Query: 721 LLHTSGSSGTPKTIKITSAQLFNRLFWQWRNLPFKSNEIVCHRTGFMFVDNIVECLSCILSTVTMVIVSPTEACDIVKLAEVIQKYSVSWILTVPSLLQKWLKQLDNYSDIXXXXXXXXXXXXXGEMLFPSLAKKCLATFNRNSCKLVNLYGSTEVCGDVCCQTLYSINDVRMNSKNKFLSVGTPISNNQVFFESNSDN------EGEVIVIGKNVS-------------------PTCGLAFQTGDVG-FIADKKLFICGRIDDMVKVNGKKIFTKDISTAV 1491
+++TSGS+G PK I + NRLFW + + +T + F ++ E ++ +V P D L ++I+ ++ + VPS+LQ +L+ S+ V SGE L L ++ +F +S +L NLYG TE DV + + + +G P++N Q++ N N GE+ + G V+ G ++TGD+G + D + GRID VK+ G +I +I A+
Sbjct: 4 MIYTSGSTGKPKGAMIEHRSIVNRLFWMQDEYKVDKTDKILQKTPYSFDVSVWEFFLPLMQGAQLVFAKPEGHKDPGYLIDLIESEKITILHFVPSMLQVFLE-----VKTKLQCKSIKKVFCSGEALPFELVQRFFKSF--DSAELHNLYGPTEAAVDVTYW------HCKREDSSGIIPIGFPVANTQIYVLDNYGNIVPDGVIGEIHIGGIQVARGYHNKESLTNEKFVKDPFSEHGKLYKTGDLGRRLKDGSVEYKGRIDFQVKIRGMRIELGEIEAAL 273
BLAST of Ss-alanyl conjugating enzyme vs. Ensembl Nematostella
Match: EDO33420 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SS95]) HSP 1 Score: 68.1662 bits (165), Expect = 1.525e-11 Identity = 104/461 (22.56%), Postives = 190/461 (41.21%), Query Frame = 1
Query: 286 PNHIAAIHNDSFLTYSMLNTKANIVAKNLIRSCNLEIPMNECLVGVLLDEGFERLYSIMACLKLGLVFVPLAKNRNSDLLKRIIDKCNLSIIIHKKCSDIDLLSNDNSIQTVLMDTLLDESKIIDSFNLLPREYNPPQSREKTVV-------LLHTSGSSGTPKTIKITSAQLFNRLFWQWRNLPFKSNEIVCHRTGFMFVDNIVECLSCILSTVTMVIVSPTEACDIVKLAEVIQKYSVSWILTVPSLLQKWLKQLDNYSDIXXXXXXXXXXXXXGEMLFPSLAKKC---LATFNRNSCKLVNLYGSTE--VCGDVCCQTLYSINDVRMNSKNKFLSVGTPISN-NQVFFESN-----SDNEGEVIVIGKNVSPTC-----------------GLAFQTGD-VGFIADKKLFICGRIDDMVKVNGKKIFTKDISTAVMVHSDVDNCYTIQLIISGRPQLV 1560
PN+IA + ++Y L+ K+N A+ LI +++ E +V +LL E ++++ LK G +V + L I+ N +I K + + +D + ++LD I S L + + P + TVV +++TSGS+G PK ++I + N + + P S + +C F ++ E + T+V P L + +++ ++ + TVP+LL+ D+ + ++ G + C L + + L+N YG TE V C +++ + K +++G P+ N N +SN + EGE+ + GK ++ G ++TGD V + D ++ GRIDD VK+ G ++ I T + +V + L S QLV
Sbjct: 42 PNNIAVELKNRQMSYEALDQKSNRFARLLIEKFGIKVE-EERVVAILLPRCPELYLTMLSVLKAGGAYVCIDPQHQISRLPYILQDTNAVALITTKQVESEFGLSD-----LTKVSILD----IQSRALNTQIASKPSNALATVVPSSHLCYIIYTSGSTGQPKGVEIEHRTVVNFIKGELEIFPVWSQDRICQGFSPSFDASVEEIWLAFGTGATLVAAPPEVMQSGPDLPDHLEQLRITVLSTVPTLLRT--------VDVTDTKLNRLKLIILG-------GEACGEDLVAWA-DGRMLINSYGPTETTVVATTC--------EIK---RGKKITIGRPLGNYNAHILDSNLVDVEGEKEGELCISGKCLARGYRNLPEKTKQQFVDHQLHGRIYRTGDLVRWTPDGEIEYLGRIDDQVKIRGFRVEIGSIETHLQRQENVKMAAVVVLSPSPGQQLV 465
BLAST of Ss-alanyl conjugating enzyme vs. Ensembl Medaka
Match: aasdh (aminoadipate-semialdehyde dehydrogenase [Source:NCBI gene;Acc:101171235]) HSP 1 Score: 57.7658 bits (138), Expect = 6.123e-8 Identity = 100/465 (21.51%), Postives = 183/465 (39.35%), Query Frame = 1
Query: 721 LLHTSGSSGTPKTIKITSAQLFNRLFWQWRNLPFKSNEIVCHRTGFMFVDNIVECLSCILSTVTMVIVSPTEACDIVKLAEVIQK-YSVSWILTVPSLLQKWLKQLDNYSDIXXXXXXXXXXXXXGEMLFPSLAKKCLATFNRNSCKLVNLYGSTEVCGDVCCQTLYSINDVRMNSKNKF-LSVGTPISNNQVFFESN-----SDNEGEVIVIGKN----------VSPTCGLAFQTGDVGFIADKKLFICGRIDDMVKVNGKKIFTKDISTAVMVHSDVDNCYTIQLIISGRPQLVSFFTTKTEIGNKTKTKMEINNI-------------------LMNHSLNVCLPR------LEYIKSIPIQPQSMKPDKKMLCKIAKKILSENRRMKTKLTHGKHXXXXXXXXXXXXXXXXXXNSQKHYIYEILAKHLSLLPD-EIDDSMKFYDIGGDSLTIVICIADLN 1986
+LHTSG++G PKT+++ + + L ++++V + F ++V+ + S ++IV +LA ++ K + V+ + PSLL ++ + + L+ SSL + GE P + +C + NLYG TEV CC Y I + + S + + +G P+ + +V ++ EG++ + G + V P G TGD I D++L GR D +K NGK++ + ++ +V C L + +L++F + + + E++ + + L+V LP LE + ++P+ P K D +A L E +R G+ + K + + L L D ++D+ F GGDSL V D+
Sbjct: 188 VLHTSGTTGPPKTVRVPHTCIVPNILHLRSLLQMSASDVVFLASPLTFDPSVVDIFLALSSGAQLLIVPSLIKKTPRRLAGLLFKDHKVTVLQVTPSLLLRFGHRTLK-EEALSPGSSLRVLALGGEACPPPALLRSWKHQENKTC-IFNLYGITEVSCWACC---YKIPETLLQSSDPGEVPLGAPLMDTEVEVRDELGRVVTEGEGQLFLGGGDRVCLLDGESTVVP--GTMRATGDWVKIRDQQLHYQGRKDRTIKRNGKRVNLDRLQQLLLGLPEVAAC---ALGLHSSSRLLAFVVSAGAAERRCSSSAELDLLSSVRQQSEEVRGAGGDGKRRILEQLSVLLPSHGIPDTLELLPALPVTPHG-KVD-----MVALMTLYERQREHLGFPRGE------------------AHKLKQRLQALWKDALGLPGDADLDEQSNFLQSGGDSLKAVRLCEDIG 618
BLAST of Ss-alanyl conjugating enzyme vs. Planmine SMEST
Match: SMESG000023215.1 (SMESG000023215.1) HSP 1 Score: 1680.61 bits (4351), Expect = 0.000e+0 Identity = 857/867 (98.85%), Postives = 861/867 (99.31%), Query Frame = 1
Query: 157 MKVMNQLSNTSHCLYGENDSYEHCNNLATYFEMNCLNDCRKDNPNHIAAIHNDSFLTYSMLNTKANIVAKNLIRSCNLEIPMNECLVGVLLDEGFERLYSIMACLKLGLVFVPLAKNRNSDLLKRIIDKCNLSIIIHKKCSDIDLLSNDNSIQTVLMDTLLDESKIIDSFNLLPREYNPPQSREKTVVLLHTSGSSGTPKTIKITSAQLFNRLFWQWRNLPFKSNEIVCHRTGFMFVDNIVECLSCILSTVTMVIVSPTEACDIVKLAEVIQKYSVSWILTVPSLLQKWLKQLDNYSDIXXXXXXXXXXXXXGEMLFPSLAKKCLATFNRNSCKLVNLYGSTEVCGDVCCQTLYSINDVRMNSKNKFLSVGTPISNNQVFFESNSDNEGEVIVIGKNVSPTCGLAFQTGDVGFIADKKLFICGRIDDMVKVNGKKIFTKDISTAVMVHSDVDNCYTIQLIISGRPQLVSFFTTKTEIGNKTKTKMEINNILMNHSLNVCLPRLEYIKSIPIQPQSMKPDKKMLCKIAKKILSENRRMKTKLTHGKHXXXXXXXXXXXXXXXXXXNSQKHYIYEILAKHLSLLPDEIDDSMKFYDIGGDSLTIVICIADLNRKGFSCTTEVFFHNHSIGELVECILEQSNSKIRNNSDYKLFKYDMKNDFTVEQIQYENKTEIIEFLVENFYTKELVVRYLNTPVKVFVEVVSEIFDLSLRSGCSFCIRNSISKSIVGLQLSEDSSYEIPNTSQLYKLDEENSLDCFLQNCKPRIAEFLKTPKLNVSVVALSGTLNKSIVIQLLYFIEKHTIDLAQCKNYKTIETINTSEATKKICSELNYRLIKSTSMSHFLNESQCQYLRSLSMSDVYGHYMVLDL 2757
MKVMNQLSNTSHCLYGENDSYEHCNNLATYFEMNCLNDCRKDNPNHIAAIHNDSFLTYSMLNTKANIVAKNLIRSCNLEIP+NECLVGVLLDEGFERLYSIMACLKLGLVFVPLAKNRNSDLLKRIIDKCNLSIIIHKKCSDIDLLSNDNSIQTVLMDTLLDESKIIDSFNLLPREYNPPQSREKTVVLLHTSGSSGTPK IKITSAQLFNRLFWQWRNLPFKSNEIVCHRTGFMFVDNIVECLSCILSTVTMVIVSPTEACDIVKLAEVIQKYSVSWILTVPSLLQKWLKQLDNYSDILALLSSLSTVVSSGEMLFPSLAKKCLATFNRNSCKLVNLYGSTEVCGDVCCQTLYSINDVRMNSKNKFLSVGTPISNNQVFFESNSDNEGEVIVIGKNVSPTCGLAFQTGDVGFIADKKL+ICGR+DDMVKVNGKKIFTKDISTAVMVHSDVDNCYTIQLIISGRPQLVSFFTTKTEIGNKTKTKMEINNILMNHSLNVCLPRLEYIKSIPIQPQSMKPDKKMLCKIAKKILSENRRMKTKLTHGKHLLSSQTTLDLTDGLIDDI SQK YIYEILAKHLSLLPDEIDDSM FYDIGGDSLTIVICIADLNRKGFSCTTEVFFHNHSIGELVECILEQSNSKIRNNSDYKLFKYDMKNDFTVEQIQYENKTEIIEFLVENFYTKELVVRYLNTPVKVFVEVVSEIFDLSLRSGCSFCIRNSISKSIVGLQLSEDSSYEIPNTSQLYKLDEENSLDCFLQNCKPRIAEFLKTPKLNVSVVALSGTLNKSIVIQLLYFIEKHTIDLAQCKNYKTIETINTSEATKKICSELNYRLIKSTS+SHFLNE QCQYLRSLSM DVYGHYMVLDL
Sbjct: 1 MKVMNQLSNTSHCLYGENDSYEHCNNLATYFEMNCLNDCRKDNPNHIAAIHNDSFLTYSMLNTKANIVAKNLIRSCNLEIPINECLVGVLLDEGFERLYSIMACLKLGLVFVPLAKNRNSDLLKRIIDKCNLSIIIHKKCSDIDLLSNDNSIQTVLMDTLLDESKIIDSFNLLPREYNPPQSREKTVVLLHTSGSSGTPKAIKITSAQLFNRLFWQWRNLPFKSNEIVCHRTGFMFVDNIVECLSCILSTVTMVIVSPTEACDIVKLAEVIQKYSVSWILTVPSLLQKWLKQLDNYSDILALLSSLSTVVSSGEMLFPSLAKKCLATFNRNSCKLVNLYGSTEVCGDVCCQTLYSINDVRMNSKNKFLSVGTPISNNQVFFESNSDNEGEVIVIGKNVSPTCGLAFQTGDVGFIADKKLYICGRVDDMVKVNGKKIFTKDISTAVMVHSDVDNCYTIQLIISGRPQLVSFFTTKTEIGNKTKTKMEINNILMNHSLNVCLPRLEYIKSIPIQPQSMKPDKKMLCKIAKKILSENRRMKTKLTHGKHLLSSQTTLDLTDGLIDDIKSQKRYIYEILAKHLSLLPDEIDDSMNFYDIGGDSLTIVICIADLNRKGFSCTTEVFFHNHSIGELVECILEQSNSKIRNNSDYKLFKYDMKNDFTVEQIQYENKTEIIEFLVENFYTKELVVRYLNTPVKVFVEVVSEIFDLSLRSGCSFCIRNSISKSIVGLQLSEDSSYEIPNTSQLYKLDEENSLDCFLQNCKPRIAEFLKTPKLNVSVVALSGTLNKSIVIQLLYFIEKHTIDLAQCKNYKTIETINTSEATKKICSELNYRLIKSTSISHFLNECQCQYLRSLSMLDVYGHYMVLDL 867 The following BLAST results are available for this feature:
BLAST of Ss-alanyl conjugating enzyme vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Ss-alanyl conjugating enzyme vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 2
BLAST of Ss-alanyl conjugating enzyme vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 1
BLAST of Ss-alanyl conjugating enzyme vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 2
BLAST of Ss-alanyl conjugating enzyme vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 4
BLAST of Ss-alanyl conjugating enzyme vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 4
BLAST of Ss-alanyl conjugating enzyme vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Ss-alanyl conjugating enzyme vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Ss-alanyl conjugating enzyme vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 1
BLAST of Ss-alanyl conjugating enzyme vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 2
BLAST of Ss-alanyl conjugating enzyme vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Ss-alanyl conjugating enzyme vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 3
BLAST of Ss-alanyl conjugating enzyme vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 1
BLAST of Ss-alanyl conjugating enzyme vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 1
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30020538 ID=SMED30020538|Name=Ss-alanyl conjugating enzyme|organism=Schmidtea mediterranea sexual|type=transcript|length=2768bpback to top protein sequence of SMED30020538-orf-1 >SMED30020538-orf-1 ID=SMED30020538-orf-1|Name=SMED30020538-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=868bp MKVMNQLSNTSHCLYGENDSYEHCNNLATYFEMNCLNDCRKDNPNHIAAIback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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