SMED30020533

Overview
NameSMED30020533
Smed IDSMED30020533
Length (bp)1981
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30020533 (SMED30020533) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30020533 (SMED30020533) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30020533 (SMED30020533) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30020533 (SMED30020533) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30020533

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
neuronSMED30020533 dd_Smed_v4_5963_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30020533 dd_Smed_v4_5963_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of SMED30020533 vs. Planmine SMEST
Match: SMESG000014554.1 (SMESG000014554.1)

HSP 1 Score: 105.145 bits (261), Expect = 3.602e-24
Identity = 76/297 (25.59%), Postives = 139/297 (46.80%), Query Frame = 3
Query:  207 YKLVIQNVDNNAKF---PYDNFVLAIELISQDTDEPIVCAITSLDYEGEYYRGFSVKNFVVGKNNMS----MMLTFSREAFNKKFIIRYGSEKSDTDKQLFFENRKKG-IIYNTNLNIYIPYKITLDATPDLGVVNKDDSMELEFQLIPEKYGLITCKATEGLPKSRVYFINGYILKGSTITHLNEEIT--SLKWKVMLEDHGKTIQCKAGSSVQNVTINVYHYVTKVHSSPQVSTKLARNSQLKCISEGGYPPPKTNWTLITGPPEAYKHYQFRDNNVTLFINNSAPINSNWTFEC 1067
            + L IQ+   N K    P D  V+ +  ++    E       +L +  + ++G  +   +  KN MS    + + F REA NK   I Y S  +   ++ + +N      +++  ++++IP  + L   P + V  KD        +I     + +C+A  G+P+ +  F +   L  + I + NE     S+  +  L+DH     C AG+  +   I ++H+ +K+H  P +   L  N  ++C ++ GYP P  ++ LI GP  A  +Y+ R N   ++I ++AP  S W F+C
Sbjct:   40 FSLDIQSRSENGKLSSDPSDTMVIRVVDLNFTEIERAYLVYGALYFMDDEFKGNLMDIKIWEKNQMSTKIDISIIFKREAHNKYLEIFYFSAPNKFVRRKYLKNLSLAKRLFHELIDLFIPSTLALTLLP-IKVDRKD--------IIINDMVMASCRAFGGIPQEKPRFEDHAQLNVTEIIY-NETANEISMNLRTSLDDHLTNFSCSAGNVTETFIICIHHHPSKIHIKPDIKDILQANRAIQCYAQDGYPQPSLHFQLIKGPSNASHYYETRKNGSEIYIRSNAPTRSAWMFKC 326          
BLAST of SMED30020533 vs. Planmine SMEST
Match: SMESG000014554.1 (SMESG000014554.1)

HSP 1 Score: 104.76 bits (260), Expect = 3.979e-24
Identity = 76/297 (25.59%), Postives = 139/297 (46.80%), Query Frame = 3
Query:  207 YKLVIQNVDNNAKF---PYDNFVLAIELISQDTDEPIVCAITSLDYEGEYYRGFSVKNFVVGKNNMS----MMLTFSREAFNKKFIIRYGSEKSDTDKQLFFENRKKG-IIYNTNLNIYIPYKITLDATPDLGVVNKDDSMELEFQLIPEKYGLITCKATEGLPKSRVYFINGYILKGSTITHLNEEIT--SLKWKVMLEDHGKTIQCKAGSSVQNVTINVYHYVTKVHSSPQVSTKLARNSQLKCISEGGYPPPKTNWTLITGPPEAYKHYQFRDNNVTLFINNSAPINSNWTFEC 1067
            + L IQ+   N K    P D  V+ +  ++    E       +L +  + ++G  +   +  KN MS    + + F REA NK   I Y S  +   ++ + +N      +++  ++++IP  + L   P + V  KD        +I     + +C+A  G+P+ +  F +   L  + I + NE     S+  +  L+DH     C AG+  +   I ++H+ +K+H  P +   L  N  ++C ++ GYP P  ++ LI GP  A  +Y+ R N   ++I ++AP  S W F+C
Sbjct:   36 FSLDIQSRSENGKLSSDPSDTMVIRVVDLNFTEIERAYLVYGALYFMDDEFKGNLMDIKIWEKNQMSTKIDISIIFKREAHNKYLEIFYFSAPNKFVRRKYLKNLSLAKRLFHELIDLFIPSTLALTLLP-IKVDRKD--------IIINDMVMASCRAFGGIPQEKPRFEDHAQLNVTEIIY-NETANEISMNLRTSLDDHLTNFSCSAGNVTETFIICIHHHPSKIHIKPDIKDILQANRAIQCYAQDGYPQPSLHFQLIKGPSNASHYYETRKNGSEIYIRSNAPTRSAWMFKC 322          
BLAST of SMED30020533 vs. Planmine SMEST
Match: SMESG000014554.1 (SMESG000014554.1)

HSP 1 Score: 104.76 bits (260), Expect = 4.060e-24
Identity = 76/297 (25.59%), Postives = 139/297 (46.80%), Query Frame = 3
Query:  207 YKLVIQNVDNNAKF---PYDNFVLAIELISQDTDEPIVCAITSLDYEGEYYRGFSVKNFVVGKNNMS----MMLTFSREAFNKKFIIRYGSEKSDTDKQLFFENRKKG-IIYNTNLNIYIPYKITLDATPDLGVVNKDDSMELEFQLIPEKYGLITCKATEGLPKSRVYFINGYILKGSTITHLNEEIT--SLKWKVMLEDHGKTIQCKAGSSVQNVTINVYHYVTKVHSSPQVSTKLARNSQLKCISEGGYPPPKTNWTLITGPPEAYKHYQFRDNNVTLFINNSAPINSNWTFEC 1067
            + L IQ+   N K    P D  V+ +  ++    E       +L +  + ++G  +   +  KN MS    + + F REA NK   I Y S  +   ++ + +N      +++  ++++IP  + L   P + V  KD        +I     + +C+A  G+P+ +  F +   L  + I + NE     S+  +  L+DH     C AG+  +   I ++H+ +K+H  P +   L  N  ++C ++ GYP P  ++ LI GP  A  +Y+ R N   ++I ++AP  S W F+C
Sbjct:   38 FSLDIQSRSENGKLSSDPSDTMVIRVVDLNFTEIERAYLVYGALYFMDDEFKGNLMDIKIWEKNQMSTKIDISIIFKREAHNKYLEIFYFSAPNKFVRRKYLKNLSLAKRLFHELIDLFIPSTLALTLLP-IKVDRKD--------IIINDMVMASCRAFGGIPQEKPRFEDHAQLNVTEIIY-NETANEISMNLRTSLDDHLTNFSCSAGNVTETFIICIHHHPSKIHIKPDIKDILQANRAIQCYAQDGYPQPSLHFQLIKGPSNASHYYETRKNGSEIYIRSNAPTRSAWMFKC 324          
BLAST of SMED30020533 vs. Planmine SMEST
Match: SMESG000014542.1 (SMESG000014542.1)

HSP 1 Score: 106.301 bits (264), Expect = 1.612e-23
Identity = 81/322 (25.16%), Postives = 150/322 (46.58%), Query Frame = 3
Query:  207 YKLVIQNVDNNAKF---PYDNFVLAIELISQDTDEPIVCAITSLDYEGEYYRGFSVKNFVVGKNNMS----MMLTFSREAFNKKFIIRYGSEKSDTDKQLFFENRKKG-IIYNTNLNIYIPYKITLDATPDLGVVNKDDSMELEFQLIPEKYGLITCKATEGLPKSRVYFINGYILKGSTITHLNEEIT--SLKWKVMLEDHGKTIQCKAGSSVQNVTINVYHYVTKVHSSPQVSTKLARNSQLKCISEGGYPPPKTNWTLITGPPEAYKHYQFRDNNVTLFINNSAPINSNWTFEC---RGMNEMPLVVQKYLPIPLPNYP 1133
            + L IQ+   N K    P D  V+ I  ++    E       +L +  + ++G  +   +  KN MS    + + F REA NK   I Y S  +   ++ + +N      +++  ++++IP  + L   P + V  K        ++I     + +C+A  G+P+ +  F +   L  + I + NE     S+  +  L+DH     C AG+  +   I ++H+ +K+H +P +   L  N  ++C  + GYP P  ++ LI GP  A  +Y+ R N   +FI +SAP  S W F+C     + E+ +  +K +   + ++P
Sbjct:   36 FSLDIQSRSENGKLSSDPSDTMVIRIVDLNFTEIERAYLVYGALYFMDDEFKGNLMDIKIWEKNQMSTKIDISIIFKREAHNKYLEIFYFSAPNKFVRRKYLKNLSLAKRLFHELIDLFIPSTLALTLLP-IKVDRK--------EIIINDMVMASCRAFSGIPQEKPRFEDHAQLNVTEIIY-NETANEISMNLRASLDDHLTNFSCSAGNVTETFIIRIHHHPSKIHITPDIKDILQANRAIQCYVQDGYPQPSLHFQLIKGPSNASHYYETRKNGSEIFIRSSAPTGSAWMFKCLMKSTIAEVAMTKEKNITFRIASHP 347          

HSP 2 Score: 77.411 bits (189), Expect = 2.122e-14
Identity = 47/167 (28.14%), Postives = 76/167 (45.51%), Query Frame = 3
Query:  624 IPEKYGLITCKATEGLPKSRVYF--INGY--------ILKGSTITHLNEEI-TSLKWKVMLEDHGKTIQCKAGSSVQNVTI--------NVYHYVTKVHSSPQVSTKLARNSQLKCISEGGYPPPKTNWTLITGPPEAYKHYQFRDNNVTLFINNSAPINSNWTFEC 1067
            I E    I C   +G P +R  +  I G         I++  T  HL E   T  +W+         I+C   S ++NV +         + ++ T ++  P +  +   NS +KC ++ GYP P+ NW LI GPP A + Y        + IN+++P+ S WT +C
Sbjct:  359 IMEPNSTIACGPADGYPVARATWEVIAGLESASLYYNIMRNGTQIHLKENCPTKSEWE---------IKCSVKSCLENVCVILNRTISFRIANHPTALYIQPIIKKRFRSNSTIKCFTKDGYPDPEINWQLIEGPPNADRFYNIPGKGDQIHINSNSPVGSKWTLKC 516          
BLAST of SMED30020533 vs. Planmine SMEST
Match: SMESG000014542.1 (SMESG000014542.1)

HSP 1 Score: 106.301 bits (264), Expect = 1.723e-23
Identity = 81/322 (25.16%), Postives = 150/322 (46.58%), Query Frame = 3
Query:  207 YKLVIQNVDNNAKF---PYDNFVLAIELISQDTDEPIVCAITSLDYEGEYYRGFSVKNFVVGKNNMS----MMLTFSREAFNKKFIIRYGSEKSDTDKQLFFENRKKG-IIYNTNLNIYIPYKITLDATPDLGVVNKDDSMELEFQLIPEKYGLITCKATEGLPKSRVYFINGYILKGSTITHLNEEIT--SLKWKVMLEDHGKTIQCKAGSSVQNVTINVYHYVTKVHSSPQVSTKLARNSQLKCISEGGYPPPKTNWTLITGPPEAYKHYQFRDNNVTLFINNSAPINSNWTFEC---RGMNEMPLVVQKYLPIPLPNYP 1133
            + L IQ+   N K    P D  V+ I  ++    E       +L +  + ++G  +   +  KN MS    + + F REA NK   I Y S  +   ++ + +N      +++  ++++IP  + L   P + V  K        ++I     + +C+A  G+P+ +  F +   L  + I + NE     S+  +  L+DH     C AG+  +   I ++H+ +K+H +P +   L  N  ++C  + GYP P  ++ LI GP  A  +Y+ R N   +FI +SAP  S W F+C     + E+ +  +K +   + ++P
Sbjct:   40 FSLDIQSRSENGKLSSDPSDTMVIRIVDLNFTEIERAYLVYGALYFMDDEFKGNLMDIKIWEKNQMSTKIDISIIFKREAHNKYLEIFYFSAPNKFVRRKYLKNLSLAKRLFHELIDLFIPSTLALTLLP-IKVDRK--------EIIINDMVMASCRAFSGIPQEKPRFEDHAQLNVTEIIY-NETANEISMNLRASLDDHLTNFSCSAGNVTETFIIRIHHHPSKIHITPDIKDILQANRAIQCYVQDGYPQPSLHFQLIKGPSNASHYYETRKNGSEIFIRSSAPTGSAWMFKCLMKSTIAEVAMTKEKNITFRIASHP 351          

HSP 2 Score: 77.411 bits (189), Expect = 2.039e-14
Identity = 47/167 (28.14%), Postives = 76/167 (45.51%), Query Frame = 3
Query:  624 IPEKYGLITCKATEGLPKSRVYF--INGY--------ILKGSTITHLNEEI-TSLKWKVMLEDHGKTIQCKAGSSVQNVTI--------NVYHYVTKVHSSPQVSTKLARNSQLKCISEGGYPPPKTNWTLITGPPEAYKHYQFRDNNVTLFINNSAPINSNWTFEC 1067
            I E    I C   +G P +R  +  I G         I++  T  HL E   T  +W+         I+C   S ++NV +         + ++ T ++  P +  +   NS +KC ++ GYP P+ NW LI GPP A + Y        + IN+++P+ S WT +C
Sbjct:  363 IMEPNSTIACGPADGYPVARATWEVIAGLESASLYYNIMRNGTQIHLKENCPTKSEWE---------IKCSVKSCLENVCVILNRTISFRIANHPTALYIQPIIKKRFRSNSTIKCFTKDGYPDPEINWQLIEGPPNADRFYNIPGKGDQIHINSNSPVGSKWTLKC 520          
The following BLAST results are available for this feature:
BLAST of SMED30020533 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020533 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020533 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020533 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020533 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020533 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020533 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020533 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020533 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020533 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020533 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020533 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020533 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020533 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000014554.13.602e-2425.59SMESG000014554.1[more]
SMESG000014554.13.979e-2425.59SMESG000014554.1[more]
SMESG000014554.14.060e-2425.59SMESG000014554.1[more]
SMESG000014542.11.612e-2325.16SMESG000014542.1[more]
SMESG000014542.11.723e-2325.16SMESG000014542.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30020533 ID=SMED30020533|Name=SMED30020533|organism=Schmidtea mediterranea sexual|type=transcript|length=1981bp
TTTTATTAATTCTAGTTTAATAAAATATTTGATTAAATTTCATAGTTTAT
ATTATTATTTTTTTTTACTTAGATAATTTATCAGTTTATATTGAAATGTC
CAATTTTTTTATAATATTATTAGGATTTATGTATTATACATATTCTGTGG
AATCGACAGTAGTTGTTAGTTTTAGTATTCCAAATCAAATTCTTCTAAAA
GTTCCATATAAATTAGTTATTCAAAATGTAGATAATAATGCAAAATTTCC
ATATGATAATTTTGTGCTCGCTATTGAATTAATAAGCCAAGATACCGATG
AACCTATTGTTTGCGCTATTACATCATTAGACTATGAAGGAGAATACTAT
CGTGGATTTTCTGTTAAAAATTTTGTAGTCGGAAAAAATAATATGTCAAT
GATGTTGACTTTCAGTCGAGAAGCATTTAATAAAAAATTTATCATTCGAT
ATGGATCAGAAAAAAGCGACACTGACAAGCAATTATTTTTTGAAAACAGA
AAAAAAGGAATAATTTACAACACAAATCTCAATATATATATTCCATACAA
AATAACTTTGGACGCAACACCAGATTTAGGAGTCGTTAATAAGGATGATT
CGATGGAATTAGAGTTCCAATTGATTCCAGAGAAATACGGCTTAATTACC
TGCAAAGCAACAGAGGGACTGCCGAAAAGTCGCGTATATTTCATAAACGG
ATACATTTTGAAAGGCTCGACAATTACACATTTAAATGAAGAAATTACAA
GTCTGAAGTGGAAAGTTATGCTGGAAGATCACGGGAAAACAATCCAATGC
AAGGCGGGCAGCAGTGTGCAAAACGTTACAATAAACGTCTATCATTATGT
TACAAAAGTGCATTCGAGTCCTCAAGTCTCGACTAAATTGGCCCGAAACT
CTCAATTAAAGTGCATTAGTGAGGGCGGATATCCCCCGCCAAAAACTAAT
TGGACATTGATTACGGGACCCCCTGAAGCTTACAAACATTATCAATTCAG
AGACAACAATGTAACATTATTTATTAACAATTCCGCTCCGATCAATTCTA
ATTGGACGTTTGAATGCCGGGGAATGAATGAAATGCCGCTGGTGGTGCAA
AAATACTTGCCCATCCCGCTGCCAAATTACCCAATAAAAACTGTCTACAA
TTTCACAATAATCGATACAGACGAATCTATTAAATCATCTCAAACTGGGC
TCGTGATCATTTTGATTATTCTTTTCACAACAATGTTGGTAATGATTGTT
CTGGGTGTAAAATATCGGTCACAAATCCGACAATTTCTAGTCGACCGTAA
TTTACAAATATGGAAATTTTTAAATTCGAGATTTTCTCAATGTCTGGCCC
GACTCAGATCATCCATCTGTGTATTAAAACCAGTTAAAGAAGCAGATAAA
CCTTCCATGACGTCAGCACCCATGTATAAATACTCGGGAGTGAAAAAGGA
CGAATCTCAGCCAAACGGTACCGAAGATACGGCATATTCTGAGATAGTGG
CGGTCGTCCAAAAGAAACACGATCCGTCATTTGGACCAATTCATCGGGTT
TGAAATCCCGGAAACCGATGGAGAAATTATACATTTTCTCAGAATAGCAT
TTACTGCCACTACTTCCGTTTGCCTAAAATAGATTGTACAGAAGGAAAGA
TTCGATTTTTGGCAACGATGTTGTGAATGTGATTTGTTGTCTGCGTGCTG
CATTGTGTATCATTATTGATCATTATTTATCAACATTTGTCAATCCAATA
TCATCCTGTTGTGTAATAAATGTGATGTTAGCTCTATTGTTAGTCGGACA
TTCTTTTATCGATTCGTCTATTTTATTGTGATCAGTTTTGCCTAGCATCG
AACTTTCTGTCAATTATCGATCGTTAATTCTCTCTATTTATTGATTATTT
TAATTATTTTTTGTTTAAATTTGAAAAATTTCCTCGATCAATTTGCAAAT
TTCGATTTTTTAGATTTCATTATTACTGTTG
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protein sequence of SMED30020533-orf-1

>SMED30020533-orf-1 ID=SMED30020533-orf-1|Name=SMED30020533-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=486bp
MSNFFIILLGFMYYTYSVESTVVVSFSIPNQILLKVPYKLVIQNVDNNAK
FPYDNFVLAIELISQDTDEPIVCAITSLDYEGEYYRGFSVKNFVVGKNNM
SMMLTFSREAFNKKFIIRYGSEKSDTDKQLFFENRKKGIIYNTNLNIYIP
YKITLDATPDLGVVNKDDSMELEFQLIPEKYGLITCKATEGLPKSRVYFI
NGYILKGSTITHLNEEITSLKWKVMLEDHGKTIQCKAGSSVQNVTINVYH
YVTKVHSSPQVSTKLARNSQLKCISEGGYPPPKTNWTLITGPPEAYKHYQ
FRDNNVTLFINNSAPINSNWTFECRGMNEMPLVVQKYLPIPLPNYPIKTV
YNFTIIDTDESIKSSQTGLVIILIILFTTMLVMIVLGVKYRSQIRQFLVD
RNLQIWKFLNSRFSQCLARLRSSICVLKPVKEADKPSMTSAPMYKYSGVK
KDESQPNGTEDTAYSEIVAVVQKKHDPSFGPIHRV*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR013783Ig-like_fold
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..20
score: 0.719
NoneNo IPR availableTMHMMTMhelixcoord: 366..388