SMED30020519

Overview
NameSMED30020519
Smed IDSMED30020519
Length (bp)1653
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30020519 (SMED30020519) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30020519 (SMED30020519) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30020519 (SMED30020519) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30020519 (SMED30020519) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30020519

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30020519SMESG000016989.1 Contig4004GPL15192PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30020519SMESG000035551.1 Contig4004GPL15192PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
nervous systemSMED30020519SMESG000035551.1 dd_Smed_v4_8719_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30020519SMESG000035551.1 dd_Smed_v4_8719_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30020519SMESG000035551.1 dd_Smed_v4_8719_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30020519 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.004560:46126..47819 -8155
v31.017353:6204..7847 -8029
Homology
BLAST of SMED30020519 vs. Planmine SMEST
Match: SMESG000035551.1 (SMESG000035551.1)

HSP 1 Score: 875.159 bits (2260), Expect = 0.000e+0
Identity = 483/488 (98.98%), Postives = 485/488 (99.39%), Query Frame = 2
Query:  101 MEPEYHIDDFLGEIKTNVINKFXXXXXXXXXXXXXXXXFSLGLYLTYTHIAQTLDLSKQLNNEFWTPLSVSNSITNLTGVCIIVCHLYVNFKYRNFIETSKSHQPEXXXXXXXXXXXXXXXXMALGSIKDTLSDSIKFSTVTFNFTSVLFFIGTLMLLIEIRNGYDTGEMPKVSLVKLTCILYCGVGILNYIIFNVSIDQLENFRFSINRTFKQQNGTQKYIVLDNSYSTLEPYMIRCIYIVGVVSLIYMYEPLKQSFNIVERFVQYENEFGSLLNTIPPKRLSRFSAGFXXXXXXXXXXXXXFKKICKSYEYYYHILGFLGHIISILLMYFHFQDLKFWKKRLKTSIIDHWFITIGLIGSLIHCISSIDALAHNLHGWSVFYIIKYVTEIFQIVIQTTYIFMFINLENYGFFKNRLMEIICPIFCLFSINFGLFIGDYIIIESTPNSQLQKGFKSQLVEVGFYNFGNMFMIFMRFHASIVFGRYVFV 1564
            MEPEYHIDDFLGEIKTNVINKFKILLIIKVIYTI IIYFSLGLYLTY HIAQTLDLSKQLNNEFWTPLSVSNSITNLTGVCIIVCHLYVNFKYRNFIETSKSHQPEILFLIILPLFVLSIIIMALGSIKDTLSDSIKFSTVTFNFTSVLFFIGTLMLLIEIRNGYDTGEMPKVSLVKLTCILYCGVGILNYIIFNVSIDQLENFRFSINRTFKQQNGTQKYIVLDNSYSTLEPYMIRCIYIVGVVSLIYMYEPLKQSFNIVERFVQYENEFGSLLNTIPPKRLSRFSAGFSLIIIILITLSSIFKKICKSYEYYYHILGFLGHIISILLMYFHFQDLKFWKKRLKTSIIDHWFITIGLIGSLIHCISSIDALA+NLHGWSVFYIIKYVTEIFQIV QTTYIFMFINLENYGFFKNRLMEIICPIFCLFSINFGLFIGDYIIIESTPNSQLQKGFKSQLVE+GFYNFGNMFMIFMRFHASIVFGRYVFV
Sbjct:    1 MEPEYHIDDFLGEIKTNVINKFKILLIIKVIYTIFIIYFSLGLYLTYKHIAQTLDLSKQLNNEFWTPLSVSNSITNLTGVCIIVCHLYVNFKYRNFIETSKSHQPEILFLIILPLFVLSIIIMALGSIKDTLSDSIKFSTVTFNFTSVLFFIGTLMLLIEIRNGYDTGEMPKVSLVKLTCILYCGVGILNYIIFNVSIDQLENFRFSINRTFKQQNGTQKYIVLDNSYSTLEPYMIRCIYIVGVVSLIYMYEPLKQSFNIVERFVQYENEFGSLLNTIPPKRLSRFSAGFSLIIIILITLSSIFKKICKSYEYYYHILGFLGHIISILLMYFHFQDLKFWKKRLKTSIIDHWFITIGLIGSLIHCISSIDALAYNLHGWSVFYIIKYVTEIFQIVTQTTYIFMFINLENYGFFKNRLMEIICPIFCLFSINFGLFIGDYIIIESTPNSQLQKGFKSQLVEIGFYNFGNMFMIFMRFHASIVFGRYVFV 488          
BLAST of SMED30020519 vs. Planmine SMEST
Match: SMESG000040945.1 (SMESG000040945.1)

HSP 1 Score: 81.6481 bits (200), Expect = 3.758e-16
Identity = 118/462 (25.54%), Postives = 217/462 (46.97%), Query Frame = 2
Query:  215 FSLGLYLTYTHIAQTLDLSKQLNNEFWTPLSVSNSITNLTGVCIIVCHLYVNFKYRNFIETSKSHQPEXXXXXXXXXXXXXXXXMALGSIKDTL-SDSIKFSTVTFNFTSVLFFIGTLMLLIEIRNGYDTGEMPKVSLVKLTCILYCGVGILNYIIFNVSIDQLENFRFSINRTFKQQNGTQKYIVLDNSYSTLEPYMIRCIYIVGVVSLIYMYEPLKQSF-----NIVERFVQYENEFGSLLNTIPPK----RLSRFSAGFXXXXXXXXXXXXXFKKICKSYEYYYHILGFLGHIISILLMYFHFQDLKFWKKRLKTSIIDHWFITIGLIGSLIHCI----SSIDALAHNLHGWSVFYIIKYVTEIFQIVIQTTYIFMFINLENYGFFKNRLMEII-CPIFCLFSINFGLFIGDYIIIESTPNSQLQKGFKSQLVEVGFYNFGNMFMIFMRFHASIVFGRY 1555
            FS G+   Y  +AQ+L    + +  F  PL+V  + TN+    +I+ ++Y NF  RNF E  K     IL+LI LP  VL ++ +++  +K+ L  ++       F+ +S++ F+  L LLIE     D+GE   ++L+ +T IL+  + ++N +I+ + IDQ++N+  ++N +  +   +  Y  L++ +      + + I I  +  + ++  PL   F     + V + VQ E         IP      +L   S   S I I+ I +  I+    K      ++L    +I S + +YF  ++++   ++ K + I + F+ +GL G  I  I    + I  +  N        I++Y T++ +   Q   +   I  +   F K      + CP+F + S    +F+  Y++     +S +   F  QL E GF + G +F +  R H +IVF  Y
Sbjct:   34 FSFGILFNYAFVAQSLSFDSKFDKVFSDPLAVVGAFTNVMSSVLIIANMYHNFNNRNFFEKHKIPNQSILYLICLPSLVLILVGLSIVIMKEVLLKENSSVGKEIFSVSSLILFLSILSLLIEANMYKDSGEKWSINLLNMTTILFTIISLVNNLIYPLLIDQVQNYIVALNVSMAKSETSNLYSALESLFELNSNLVYKIIIIFNISCIFFLINPLVYHFKNLQNSKVSQLVQEE---------IPEDEEQPKLPNSSLILSFIFIVAICVCIIYDWTSKQSLELINLLIVSTYIASFMHIYFILKNIQKCPRKDKVNSIHYVFMWLGLFGQFIQSIAGSYAGIRLMLSNSDQNYWTRILQYFTQVIECFAQNICVTFIIERKWQPFNKKLWYSFLSCPLFLVCSNTGFMFLSFYLLYS---HSAVSGQFHVQLFESGFNHLGMIFSVLHRIHGAIVFVYY 483          
BLAST of SMED30020519 vs. Planmine SMEST
Match: SMESG000051898.1 (SMESG000051898.1)

HSP 1 Score: 69.707 bits (169), Expect = 2.282e-12
Identity = 112/463 (24.19%), Postives = 213/463 (46.00%), Query Frame = 2
Query:  215 FSLGLYLTYTHIAQTLDLSKQLNNEF-WTPLSVSNSITNLTGVCIIVCHLYVNFKYRNFIETSKSHQPEXXXXXXXXXXXXXXXXMALGSIKDT-LSDSIKFSTVTFNFTSVLFFIGTLMLLIEIRNGYDTGEMPKVSLVKLTCILYCGVGILNYIIFNVSIDQLENFRFSINRTFKQQNGTQKYIVLDNS----YSTLEPYMIRCIYIVGVVSLIYMYEPLKQSFNIVERFVQYENEFGSLL-NTIPPKRLSR-------FSAGFXXXXXXXXXXXXXFKKICKSYEYYYHIL---GFLGHIISILLMYFHFQDLKFWKKRL-KTSIIDHWFITIGLIGSLIHCISSIDA----LAHNLHGWSVFYIIKYVTEIFQIVIQTTYIFMFIN-LENYGFFKNRLMEIICPIFCLFSINFGLFIGDYIIIESTPNSQLQKGFKSQLVEVGFYNFGNMFMIFMRFHA 1534
            FS+GL +TY     TL L+     EF W PL +  S+ NL  V +I+  LY+N KYRN          +IL+   +P  +  I  + +  +K   +++      V F FTSV++F+  ++ + E  + +D GE   ++ V +   L     ++  I+FN S++Q++ F   IN++   Q+       +       + ++  ++ + I + G+ S +++++ L +++ +  +F +Y+ +   L  N     R S+       +   FS ++  ++ L+       +S EY  HIL       +I  I L  F  ++L F    + +  + +   I +GL G L+HC + I A    + + ++   +  +IKYV +I   ++Q+ +  + +  L N    KN    +    F +   NF     D I++        +   K +L    F+NFGN+F +  R H 
Sbjct:   24 FSIGLLITYGW--NTLTLASNSFLEFVWAPLYICGSVFNLLVVFLILFALYMNHKYRNLFNKCPMENQDILYYFCIPATIFVISTLIITLVKSLYIAECNGKMIVLFIFTSVIYFVVIVVFIAETNSVFDFGESWALNFVNMMVSLIIVSSLVFNIVFNTSLEQMDYFAAVINKSLINQSCIDNLPFIAQRTYYLWVSVRIFLYKSIVLFGLFSTVFIFKSLYRNY-LYLQFYKYDGKTNDLKSNDEDVNRNSQKNNLKKLYLKIFSTLLFGILFLAINIAVYIRSKEYLEHILIGMEMFSYISMIGLTIFMMKNLIFTDFSISRIYVFNEVIIWLGLCGQLVHCFAVIAAYIYLMYNEVNAIYIAILIKYVLQILSNIVQSVFEAILVEKLWNNKIGKN-FSTMAFGAFYIAVNNFVYIFTDLILVYKEFLLPFEGSVKLKLFVCLFFNFGNIFSVLQRIHG 482          
BLAST of SMED30020519 vs. Planmine SMEST
Match: SMESG000040908.1 (SMESG000040908.1)

HSP 1 Score: 61.6178 bits (148), Expect = 7.770e-10
Identity = 48/171 (28.07%), Postives = 82/171 (47.95%), Query Frame = 2
Query: 1067 HIISILLMYFHFQDLKFWKKRLKT-SIIDHWFITIGLIGSLIHCISSIDALAHNLHGWSVFYIIKYVTEIFQIVIQTTYIFMFINLENYGFFKNRLMEIICPIFCLFSINFG-LFIGDYIIIESTPNSQLQKGFKS-------QLVEVGFYNFGNMFMIFMRFHASIVFGR 1552
            ++ISI+L+     ++K W+K  +  +I+D     IG+ G L+   + I    +     S F +I+Y   I QI++Q   + + ++ +          E ICP   + SIN G LF+  + +I    N+  QK F S       Q+   GF +FG    I  R H+ ++FG+
Sbjct:  333 YLISIVLLILQIFEMKRWRKNEENFNIVDKILTWIGVCGQLMISTAQISLNIYKFEPASAFLLIEYFFLIVQILLQYVTVTLALDRQCIPPKIKTWQEFICPAVGILSINLGLLFLSLFDVI----NANSQKSFNSYETDMFIQIFGAGFQSFGETLSILQRLHSLLIFGK 499          
BLAST of SMED30020519 vs. Planmine SMEST
Match: SMESG000040928.1 (SMESG000040928.1)

HSP 1 Score: 56.6102 bits (135), Expect = 2.759e-8
Identity = 88/373 (23.59%), Postives = 156/373 (41.82%), Query Frame = 2
Query:  536 TSVLFFIGTLMLLIEIRNGYDTGEMPKVSLVKLTCILYCGVGILNYIIFNVSIDQLENF-RFSINRTFKQ---QNGTQKYIVLDNSYSTLEPYMIRCIYIVGVVSLIYMYEP--------LKQSFNIVERF----VQYENE-----FGSLLNTIPPKRLSRFSAGFXXXXXXXXXXXXXFKKICKSYEYYYHIL---GFLGHIISILLMYFHFQDLKFWKKRLK-TSIIDHWFITIGLIGSLIHCISSIDALAHNLHG--WSVFYIIKYVTEIFQIVIQTTYIFMFINLENYGFFKNRLMEIICPIFCLFSINFGLFIGDYIIIESTPNSQLQKGFKS--QLVEVGFYNFGNMFMIFMRFHASIVF----GRY 1555
            T  +FFIGT+ L + +     T    K+   K   +L   +G+  Y +FN+   Q+++F + + N +F      N + +   LD+    LE Y+     I   VS  Y+               F+   R     + +EN        S ++     ++  FS    ++   +  +  +     KS E    I     F  +I+SI+++     ++K W K+ K  +++D   + +G  G +   IS+     +N     +S+  II+ +  I QI +Q   I + +  +          E ICP F LF  N G F   + + E   N+ +Q+ F    ++   GFY+      I  RFH+SI+F    G+Y
Sbjct:  129 TESVFFIGTMTLNMYMITTRRTFNKTKIKEYKSFILLISAIGMFKYFLFNIVTHQIQSFIKVAENYSFYSYFYNNYSMENRNLDSHSPDLEIYLQPLTIIFCFVSFSYLIRSEFSKTLGDTDTQFDECSRLRGDSLSHENRESIESTNSNISNYYYFKIPSFSIILGILFFTMTLILVLIASTLKSSESGKTITVSYAFSIYIVSIIILVVQMFEMKRWPKKTKIENVVDPALLYLGTAGQIFVSISTAKLYMYNYQNKEFSILTIIQNLILIVQISLQFLVIRLSLERKWIPPVVKTWQEFICPSFTLFCFNLGYF--GFSVFEIMNNNIVQESFPLFLKIFGNGFYSISMTLAILQRFHSSIIFYKLIGKY 499          
The following BLAST results are available for this feature:
BLAST of SMED30020519 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020519 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020519 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020519 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020519 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020519 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020519 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020519 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020519 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020519 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020519 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020519 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020519 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020519 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000035551.10.000e+098.98SMESG000035551.1[more]
SMESG000040945.13.758e-1625.54SMESG000040945.1[more]
SMESG000051898.12.282e-1224.19SMESG000051898.1[more]
SMESG000040908.17.770e-1028.07SMESG000040908.1[more]
SMESG000040928.12.759e-823.59SMESG000040928.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30020519 ID=SMED30020519|Name=SMED30020519|organism=Schmidtea mediterranea sexual|type=transcript|length=1653bp
CAGGTCGCATCAACACATAGATAACAAGTGAAATAATATTTATAGATTTT
CAGACATTATCGATTTATAATAACATTAAATTCAATAGTTTAATCTCCAA
ATGGAGCCTGAATATCATATTGATGATTTTTTGGGTGAAATTAAAACAAA
TGTCATCAATAAATTCAAAATTCTGCTTATTATAAAGGTAATTTATACGA
TTTGCATAATTTATTTTTCACTCGGTCTCTATTTGACCTATACGCACATT
GCTCAGACTTTGGATTTATCTAAACAGTTAAACAATGAATTTTGGACACC
TTTATCGGTTTCCAATTCAATAACAAATCTTACTGGAGTTTGTATAATTG
TTTGTCATCTCTATGTCAATTTCAAGTATAGAAATTTTATTGAAACTTCA
AAATCTCATCAGCCAGAAATTCTATTCTTGATCATTCTACCACTTTTTGT
CTTGTCCATTATCATCATGGCGCTTGGTTCGATTAAGGACACGCTGAGTG
ACTCTATAAAATTTTCAACAGTCACATTTAATTTCACATCGGTTTTGTTT
TTCATTGGCACATTAATGTTACTTATTGAAATCAGAAACGGTTACGATAC
TGGAGAAATGCCCAAAGTAAGTTTAGTAAAACTAACGTGCATTTTGTATT
GTGGTGTTGGCATTTTGAATTACATAATTTTCAATGTGTCCATAGATCAA
CTAGAGAACTTTCGATTTTCAATCAACAGAACCTTCAAGCAACAAAATGG
TACTCAGAAATACATCGTTTTGGATAACTCATATTCAACACTAGAACCTT
ACATGATTCGATGTATTTATATAGTAGGAGTGGTTTCTTTAATTTACATG
TACGAGCCGCTTAAACAAAGTTTTAATATTGTTGAAAGATTTGTTCAATA
TGAAAATGAATTTGGAAGTTTACTTAACACCATTCCACCGAAAAGACTAT
CAAGATTTTCCGCTGGATTTTCATTAATAATAATAATTCTGATTACTTTA
TCATCGATATTTAAAAAGATATGCAAAAGCTATGAATACTATTACCATAT
CCTAGGATTCCTCGGTCATATAATATCGATATTGTTAATGTATTTCCATT
TTCAAGATCTGAAATTTTGGAAGAAACGCCTGAAAACCTCGATAATCGAC
CACTGGTTCATAACAATCGGTTTAATTGGCAGTCTCATTCACTGTATATC
AAGCATAGACGCTCTCGCTCACAACCTACATGGGTGGTCGGTTTTTTACA
TCATTAAATATGTTACGGAAATATTCCAAATTGTCATACAAACAACATAC
ATATTTATGTTTATCAATTTGGAAAATTATGGATTTTTTAAAAACAGGTT
AATGGAAATTATCTGTCCAATCTTTTGTTTGTTTTCGATCAATTTTGGAT
TGTTCATTGGGGATTACATCATTATAGAATCAACACCAAACTCTCAACTC
CAAAAAGGTTTCAAAAGTCAATTAGTAGAAGTCGGTTTCTACAATTTTGG
GAACATGTTTATGATTTTTATGCGATTCCATGCATCGATTGTGTTTGGAC
GTTATGTTTTCGTGAATAACTAGTCTTTAATTTTTATCAGCAATAATATT
AACTATATTACATATTTAATTAATAAACAAAATTTTGTTTTTATTGTTTA
AAA
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protein sequence of SMED30020519-orf-1

>SMED30020519-orf-1 ID=SMED30020519-orf-1|Name=SMED30020519-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=491bp
MEPEYHIDDFLGEIKTNVINKFKILLIIKVIYTICIIYFSLGLYLTYTHI
AQTLDLSKQLNNEFWTPLSVSNSITNLTGVCIIVCHLYVNFKYRNFIETS
KSHQPEILFLIILPLFVLSIIIMALGSIKDTLSDSIKFSTVTFNFTSVLF
FIGTLMLLIEIRNGYDTGEMPKVSLVKLTCILYCGVGILNYIIFNVSIDQ
LENFRFSINRTFKQQNGTQKYIVLDNSYSTLEPYMIRCIYIVGVVSLIYM
YEPLKQSFNIVERFVQYENEFGSLLNTIPPKRLSRFSAGFSLIIIILITL
SSIFKKICKSYEYYYHILGFLGHIISILLMYFHFQDLKFWKKRLKTSIID
HWFITIGLIGSLIHCISSIDALAHNLHGWSVFYIIKYVTEIFQIVIQTTY
IFMFINLENYGFFKNRLMEIICPIFCLFSINFGLFIGDYIIIESTPNSQL
QKGFKSQLVEVGFYNFGNMFMIFMRFHASIVFGRYVFVNN*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000070intestinal phagocyte
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableTMHMMTMhelixcoord: 286..303
NoneNo IPR availableTMHMMTMhelixcoord: 67..89
NoneNo IPR availableTMHMMTMhelixcoord: 25..47
NoneNo IPR availableTMHMMTMhelixcoord: 394..413
NoneNo IPR availableTMHMMTMhelixcoord: 138..160
NoneNo IPR availableTMHMMTMhelixcoord: 233..251
NoneNo IPR availableTMHMMTMhelixcoord: 466..488
NoneNo IPR availableTMHMMTMhelixcoord: 106..128
NoneNo IPR availableTMHMMTMhelixcoord: 420..442
NoneNo IPR availableTMHMMTMhelixcoord: 173..195
NoneNo IPR availableTMHMMTMhelixcoord: 352..374
NoneNo IPR availableTMHMMTMhelixcoord: 313..332