SMED30020464

Overview
NameSMED30020464
Smed IDSMED30020464
Length (bp)1235
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30020464 (SMED30020464) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30020464 (SMED30020464) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30020464 (SMED30020464) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30020464 (SMED30020464) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30020464

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30020464h1SMcG0003342 SmedASXL_016787SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
cephalic gangliaSMED30020464h1SMcG0003342 dd_Smed_v4_14808_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30020464h1SMcG0003342 dd_Smed_v4_14808_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30020464h1SMcG0003342 dd_Smed_v6_14808_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
zeta neoblastSMED30020464h1SMcG0003342 dd_Smed_v4_14808_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30020464 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.005425:25379..36832 -4103
v31.003057:3695..13995 +4042
Homology
BLAST of SMED30020464 vs. Planmine SMEST
Match: SMESG000013963.1 (SMESG000013963.1)

HSP 1 Score: 679.478 bits (1752), Expect = 0.000e+0
Identity = 377/397 (94.96%), Postives = 378/397 (95.21%), Query Frame = 2
Query:   35 MDVPIYKLDLNENSFGFDKNPNXXXXXXXXXHNVQKDSSDSNLFSQESLLNASNDKNESFWQSLRNLWRKKDCDEASVVNGNNLYHIISKIMTIIDQLENNVNDISSKVNSIESIINIFDNKVIKDVLKSTQSLNKKSEKTKIQCTDILSDMEFLKEGINSNLIAIQSAVEREELRNNADEISNDLSAQILMKFQSILENCLSELSADILVQWNDVLYNIYSDSSTRPXXXXXXXXXXXXXSPGIFLINSLRRSERLQNRNDLELTSFNRKEIMLIKENRFENIPNKSKSTKKVLPNKGVNNPPEINSHKSIPIKRSLEIFKYYEESQSDLISNELRFIYVQIISPNFIYYRKQKLTNKRSNVKIFKKDKSYDMVISANSCYDSEADLISLRKLFNC 1225
            MDVPIYKLDLNENSFGFDKNPNKKGKKSSSSHNVQKDSSDSNLFSQESLLNASNDKNESFWQSLRNLWRKKDCDEASVVNGNNLYHIISKIMTIIDQLENNVNDISSKVNSIESIINIFDNKVIKDVLKSTQSLNKKSEKTKIQCTDILSDMEFLKEGINSNLIAIQSAVE EELRNNADEISNDLSAQILMKFQSILENCLSELSADILVQWNDVLYNIYSDSSTRPKKKKEKHIKEIRKSPG FLINSLRRSERLQNRNDLELTSFNRKEIMLIKENRFENIPNKSKSTKKVLP+KGVNNPPEINSHKSIPIKRSLEIFKYYEESQSDLISNE                 KQKLTNKRSNVKIFKKDKSYDMVISANSCYDSEADLISLRKLFNC
Sbjct:    1 MDVPIYKLDLNENSFGFDKNPNKKGKKSSSSHNVQKDSSDSNLFSQESLLNASNDKNESFWQSLRNLWRKKDCDEASVVNGNNLYHIISKIMTIIDQLENNVNDISSKVNSIESIINIFDNKVIKDVLKSTQSLNKKSEKTKIQCTDILSDMEFLKEGINSNLIAIQSAVESEELRNNADEISNDLSAQILMKFQSILENCLSELSADILVQWNDVLYNIYSDSSTRPKKKKEKHIKEIRKSPGNFLINSLRRSERLQNRNDLELTSFNRKEIMLIKENRFENIPNKSKSTKKVLPSKGVNNPPEINSHKSIPIKRSLEIFKYYEESQSDLISNE-----------------KQKLTNKRSNVKIFKKDKSYDMVISANSCYDSEADLISLRKLFNC 380          
BLAST of SMED30020464 vs. Planmine SMEST
Match: SMESG000013963.1 (SMESG000013963.1)

HSP 1 Score: 643.269 bits (1658), Expect = 0.000e+0
Identity = 361/397 (90.93%), Postives = 363/397 (91.44%), Query Frame = 2
Query:   35 MDVPIYKLDLNENSFGFDKNPNXXXXXXXXXHNVQKDSSDSNLFSQESLLNASNDKNESFWQSLRNLWRKKDCDEASVVNGNNLYHIISKIMTIIDQLENNVNDISSKVNSIESIINIFDNKVIKDVLKSTQSLNKKSEKTKIQCTDILSDMEFLKEGINSNLIAIQSAVEREELRNNADEISNDLSAQILMKFQSILENCLSELSADILVQWNDVLYNIYSDSSTRPXXXXXXXXXXXXXSPGIFLINSLRRSERLQNRNDLELTSFNRKEIMLIKENRFENIPNKSKSTKKVLPNKGVNNPPEINSHKSIPIKRSLEIFKYYEESQSDLISNELRFIYVQIISPNFIYYRKQKLTNKRSNVKIFKKDKSYDMVISANSCYDSEADLISLRKLFNC 1225
            MDVPIYKLDLNENSFGFDKNPNKKGKKSSSSHNVQKDSSDSNLFSQESLLNASNDKNESFWQSLRNLWRKKDCDEASVVNGNNLYHIISKIMTIIDQLENNVNDISSKVNSIESIINIFDNKVIKDVLKSTQSLNKKSEKTKIQCTDILSDMEFLKEGINSNLIAIQSAVE EELRNNADEISNDLSAQILMKFQSILENCLSELSADILVQWNDVLYNIYSDSSTRPKKKKEKHIKEIRKSPG FLINSLRRSERLQNRNDLELTSFNRKEIMLIKENRFENIPNKSKSTKKVLP+KGVNNPPEINSHKSIPIKRSLE+                                KQKLTNKRSNVKIFKKDKSYDMVISANSCYDSEADLISLRKLFNC
Sbjct:    1 MDVPIYKLDLNENSFGFDKNPNKKGKKSSSSHNVQKDSSDSNLFSQESLLNASNDKNESFWQSLRNLWRKKDCDEASVVNGNNLYHIISKIMTIIDQLENNVNDISSKVNSIESIINIFDNKVIKDVLKSTQSLNKKSEKTKIQCTDILSDMEFLKEGINSNLIAIQSAVESEELRNNADEISNDLSAQILMKFQSILENCLSELSADILVQWNDVLYNIYSDSSTRPKKKKEKHIKEIRKSPGNFLINSLRRSERLQNRNDLELTSFNRKEIMLIKENRFENIPNKSKSTKKVLPSKGVNNPPEINSHKSIPIKRSLEM--------------------------------KQKLTNKRSNVKIFKKDKSYDMVISANSCYDSEADLISLRKLFNC 365          
BLAST of SMED30020464 vs. Planmine SMEST
Match: SMESG000013955.1 (SMESG000013955.1)

HSP 1 Score: 445.277 bits (1144), Expect = 1.161e-157
Identity = 248/275 (90.18%), Postives = 252/275 (91.64%), Query Frame = 2
Query:  401 VIKDVLKSTQSLNKKSEKTKIQCTDILSDMEFLKEGINSNLIAIQSAVEREELRNNADEISNDLSAQILMKFQSILENCLSELSADILVQWNDVLYNIYSDSSTRPXXXXXXXXXXXXXSPGIFLINSLRRSERLQNRNDLELTSFNRKEIMLIKENRFENIPNKSKSTKKVLPNKGVNNPPEINSHKSIPIKRSLEIFKYYEESQSDLISNELRFIYVQIISPNFIYYRKQKLTNKRSNVKIFKKDKSYDMVISANSCYDSEADLISLRKLFNC 1225
            V K++LKSTQSLNKKSEKTKIQCTDILSDMEFLKEGIN+NLIAIQSAVE EELRNNADE+SNDLSAQILMKFQSILENCLSELSADILVQWNDVLYNIYSDSSTRPKKKKEKHIKEI KSPG FLINSLRRSERLQNRNDLELTSFNRKEIMLIKENRFENIPNKSKSTKKVLPNKGVNNPPEINSHKSIPIKRSLEIFKYYEESQSDLISNE                 KQKLTNKRSNVKIFKKDKS DMVISANS YDSEADLISLRKLFNC
Sbjct:    4 VDKNILKSTQSLNKKSEKTKIQCTDILSDMEFLKEGINNNLIAIQSAVESEELRNNADELSNDLSAQILMKFQSILENCLSELSADILVQWNDVLYNIYSDSSTRPKKKKEKHIKEIHKSPGNFLINSLRRSERLQNRNDLELTSFNRKEIMLIKENRFENIPNKSKSTKKVLPNKGVNNPPEINSHKSIPIKRSLEIFKYYEESQSDLISNE-----------------KQKLTNKRSNVKIFKKDKSCDMVISANSYYDSEADLISLRKLFNC 261          
BLAST of SMED30020464 vs. Planmine SMEST
Match: SMESG000041991.1 (SMESG000041991.1)

HSP 1 Score: 200.675 bits (509), Expect = 5.505e-63
Identity = 108/128 (84.38%), Postives = 122/128 (95.31%), Query Frame = 2
Query:   35 MDVPIYKLDLNENSFGFDKNPNXXXXXXXXXHNVQKDSSDSNLFSQESLLNASNDKNESFWQSLRNLWRKKDCDEASVVNGNNLYHIISKIMTIIDQLENNVNDISSKVNSIESIINIFDNKVIKDVL 418
            MDVPIYKLDL++NSFG D+NPNKKGKKSSSSHNVQKDSSDSNLFS+ESLLNASNDKNESFWQ LRNLWRKKDC EAS+VNGNN YHIISKI TIIDQLENN++DISSK+NS++ IINI+DNKVIK+++
Sbjct:    1 MDVPIYKLDLHKNSFGLDENPNKKGKKSSSSHNVQKDSSDSNLFSKESLLNASNDKNESFWQILRNLWRKKDCHEASLVNGNNFYHIISKITTIIDQLENNIDDISSKINSMKFIINIYDNKVIKELV 128          
BLAST of SMED30020464 vs. Planmine SMEST
Match: SMESG000053896.1 (SMESG000053896.1)

HSP 1 Score: 189.119 bits (479), Expect = 1.617e-58
Identity = 101/128 (78.91%), Postives = 115/128 (89.84%), Query Frame = 2
Query:   35 MDVPIYKLDLNENSFGFDKNPNXXXXXXXXXHNVQKDSSDSNLFSQESLLNASNDKNESFWQSLRNLWRKKDCDEASVVNGNNLYHIISKIMTIIDQLENNVNDISSKVNSIESIINIFDNKVIKDVL 418
            M+VPIYKLDL++N FGFD+NP KKGK S  S+NVQ D SDSNLFSQESLLNASNDKNE+FWQS R LWRKKDC+EAS+VNGNN YH ISKIMTIIDQLENNV+DISSKVNS+ SIINI+DNK IK+++
Sbjct:    1 MNVPIYKLDLHKNCFGFDENPIKKGKISLFSYNVQNDRSDSNLFSQESLLNASNDKNENFWQSFRYLWRKKDCNEASLVNGNNFYHKISKIMTIIDQLENNVDDISSKVNSMASIINIYDNKEIKELV 128          
The following BLAST results are available for this feature:
BLAST of SMED30020464 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020464 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020464 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020464 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020464 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020464 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020464 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020464 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020464 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020464 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020464 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020464 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020464 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020464 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000013963.10.000e+094.96SMESG000013963.1[more]
SMESG000013963.10.000e+090.93SMESG000013963.1[more]
SMESG000013955.11.161e-15790.18SMESG000013955.1[more]
SMESG000041991.15.505e-6384.38SMESG000041991.1[more]
SMESG000053896.11.617e-5878.91SMESG000053896.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30020464 ID=SMED30020464|Name=SMED30020464|organism=Schmidtea mediterranea sexual|type=transcript|length=1235bp
CTAAAATTTGTTATTTTAAAAATTTTAACTAAAAATGGATGTTCCTATTT
ATAAGTTAGATTTGAACGAAAACTCATTTGGTTTTGATAAAAATCCTAAT
AAAAAAGGTAAAAAATCTTCCTCTAGTCATAATGTGCAGAAAGACAGTAG
TGATTCAAATTTATTTTCTCAAGAAAGTCTCTTAAATGCATCTAATGATA
AAAATGAAAGTTTCTGGCAAAGTTTGCGCAATTTATGGCGAAAGAAAGAT
TGTGATGAAGCTTCTGTAGTCAATGGAAATAACCTTTATCATATAATTTC
AAAAATAATGACAATAATTGATCAATTGGAAAATAATGTCAATGATATAT
CTTCGAAGGTTAATAGTATAGAATCCATTATTAATATATTTGACAATAAA
GTGATTAAAGATGTATTGAAATCTACCCAAAGTTTAAACAAAAAATCGGA
AAAGACAAAAATTCAATGTACTGATATTCTTTCCGACATGGAATTTCTAA
AGGAAGGTATCAATAGTAATTTAATCGCTATTCAATCAGCAGTGGAAAGG
GAAGAATTAAGAAATAACGCGGATGAGATATCGAATGATTTATCAGCTCA
AATTCTAATGAAATTTCAATCAATTTTAGAAAATTGTCTAAGTGAATTGT
CAGCAGACATTCTTGTGCAGTGGAACGATGTTTTATATAATATCTATTCC
GATTCTTCTACAAGACCTAAAAAGAAAAAAGAAAAGCATATTAAAGAAAT
TCGTAAAAGTCCAGGAATTTTTTTAATCAACTCTCTAAGGCGATCTGAAC
GATTGCAAAATAGAAATGATCTGGAATTAACTTCTTTTAATAGGAAAGAA
ATCATGCTTATAAAAGAAAATAGATTTGAAAATATTCCAAACAAGAGTAA
ATCGACTAAGAAAGTTCTCCCTAATAAAGGTGTCAATAATCCTCCGGAAA
TCAATTCCCACAAATCAATTCCTATTAAGCGTTCTTTAGAAATTTTCAAA
TACTATGAAGAAAGTCAAAGTGATTTAATTAGCAATGAGTTACGTTTTAT
TTACGTTCAAATTATATCACCGAATTTCATTTATTATAGGAAACAGAAAT
TGACTAACAAGAGAAGTAATGTGAAAATATTTAAAAAGGACAAAAGTTAC
GATATGGTTATTAGTGCTAATTCGTGTTACGATAGTGAAGCGGATTTGAT
TTCTTTGAGAAAATTGTTTAATTGTTAAATATATC
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protein sequence of SMED30020464-orf-1

>SMED30020464-orf-1 ID=SMED30020464-orf-1|Name=SMED30020464-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=398bp
MDVPIYKLDLNENSFGFDKNPNKKGKKSSSSHNVQKDSSDSNLFSQESLL
NASNDKNESFWQSLRNLWRKKDCDEASVVNGNNLYHIISKIMTIIDQLEN
NVNDISSKVNSIESIINIFDNKVIKDVLKSTQSLNKKSEKTKIQCTDILS
DMEFLKEGINSNLIAIQSAVEREELRNNADEISNDLSAQILMKFQSILEN
CLSELSADILVQWNDVLYNIYSDSSTRPKKKKEKHIKEIRKSPGIFLINS
LRRSERLQNRNDLELTSFNRKEIMLIKENRFENIPNKSKSTKKVLPNKGV
NNPPEINSHKSIPIKRSLEIFKYYEESQSDLISNELRFIYVQIISPNFIY
YRKQKLTNKRSNVKIFKKDKSYDMVISANSCYDSEADLISLRKLFNC*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000014zeta neoblast
PLANA:0000420parapharyngeal region
PLANA:0000429neoblast
PLANA:0002109X1 cell