SAM and SH3 domain-containing protein

Overview
NameSAM and SH3 domain-containing protein
Smed IDSMED30020445
Length (bp)2009
Neoblast Clusters

Zeng et. al., 2018



 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30020445

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
cephalic gangliaSMED30020445SMESG000011174.1 dd_Smed_v4_16294_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30020445SMESG000011174.1 dd_Smed_v4_16294_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30020445SMESG000011174.1 Contig40774newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30020445SMESG000011174.1 Contig40774uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SAM and SH3 domain-containing protein vs. Ensembl Human
Match: SASH1 (SAM and SH3 domain containing 1 [Source:HGNC Symbol;Acc:HGNC:19182])

HSP 1 Score: 69.3218 bits (168), Expect = 1.272e-11
Identity = 41/104 (39.42%), Postives = 58/104 (55.77%), Query Frame = 1 
Query: 1423 VERKLQFEQIDFTDIPYSDQKGNSGIPLRLIERYAYELDRDIRDVALAMETSRSDKLL-EHNRDVRSNMDAILSDQFLNCDGIKTDSLDSFLTSIGLPMYKGKI 1731
             E KL  E ID T+ PYSD+ G  GIP  L++RYA +LD+  RDVA  M+  R  +L  +H   + S     +  + ++   I   S+  +L SIGLPMY G +
Sbjct:  507 TENKLHAEGIDLTEEPYSDKHGRCGIPEALVQRYAEDLDQPERDVAANMDQIRVKQLRKQHRMAIPSGGLTEICRKPVSPGCIS--SVSDWLISIGLPMYAGTL 608
[Close]
BLAST of SAM and SH3 domain-containing protein vs. Ensembl Human
Match: SASH1 (SAM and SH3 domain containing 1 [Source:HGNC Symbol;Acc:HGNC:19182])

HSP 1 Score: 68.1662 bits (165), Expect = 3.883e-11
Identity = 41/104 (39.42%), Postives = 58/104 (55.77%), Query Frame = 1 
Query: 1423 VERKLQFEQIDFTDIPYSDQKGNSGIPLRLIERYAYELDRDIRDVALAMETSRSDKLL-EHNRDVRSNMDAILSDQFLNCDGIKTDSLDSFLTSIGLPMYKGKI 1731
             E KL  E ID T+ PYSD+ G  GIP  L++RYA +LD+  RDVA  M+  R  +L  +H   + S     +  + ++   I   S+  +L SIGLPMY G +
Sbjct: 1097 TENKLHAEGIDLTEEPYSDKHGRCGIPEALVQRYAEDLDQPERDVAANMDQIRVKQLRKQHRMAIPSGGLTEICRKPVSPGCIS--SVSDWLISIGLPMYAGTL 1198
[Close]
BLAST of SAM and SH3 domain-containing protein vs. Ensembl Zebrafish
Match: sash1b (SAM and SH3 domain containing 1b [Source:ZFIN;Acc:ZDB-GENE-110228-1])

HSP 1 Score: 59.3066 bits (142), Expect = 1.356e-8
Identity = 26/62 (41.94%), Postives = 43/62 (69.35%), Query Frame = 1 
Query: 1423 VERKLQFEQIDFTDIPYSDQKGNSGIPLRLIERYAYELDRDIRDVALAMETSRSDKLLEHNR 1608
            VE KL  + ID T+ PYSD+ G  G+P  L++RY+ +LD+ + DVA+ ++ +R  +LL+ +R
Sbjct:  846 VEEKLCSDGIDLTEEPYSDKHGRYGVPFSLVQRYSEDLDKPLGDVAILIDQTRVQQLLKQHR 907
[Close]
BLAST of SAM and SH3 domain-containing protein vs. Ensembl Zebrafish
Match: sash1b (SAM and SH3 domain containing 1b [Source:ZFIN;Acc:ZDB-GENE-110228-1])

HSP 1 Score: 59.3066 bits (142), Expect = 1.415e-8
Identity = 26/62 (41.94%), Postives = 43/62 (69.35%), Query Frame = 1 
Query: 1423 VERKLQFEQIDFTDIPYSDQKGNSGIPLRLIERYAYELDRDIRDVALAMETSRSDKLLEHNR 1608
            VE KL  + ID T+ PYSD+ G  G+P  L++RY+ +LD+ + DVA+ ++ +R  +LL+ +R
Sbjct:  868 VEEKLCSDGIDLTEEPYSDKHGRYGVPFSLVQRYSEDLDKPLGDVAILIDQTRVQQLLKQHR 929
[Close]
BLAST of SAM and SH3 domain-containing protein vs. Ensembl Zebrafish
Match: sash1b (SAM and SH3 domain containing 1b [Source:ZFIN;Acc:ZDB-GENE-110228-1])

HSP 1 Score: 59.3066 bits (142), Expect = 1.543e-8
Identity = 26/62 (41.94%), Postives = 43/62 (69.35%), Query Frame = 1 
Query: 1423 VERKLQFEQIDFTDIPYSDQKGNSGIPLRLIERYAYELDRDIRDVALAMETSRSDKLLEHNR 1608
            VE KL  + ID T+ PYSD+ G  G+P  L++RY+ +LD+ + DVA+ ++ +R  +LL+ +R
Sbjct:  956 VEEKLCSDGIDLTEEPYSDKHGRYGVPFSLVQRYSEDLDKPLGDVAILIDQTRVQQLLKQHR 1017
[Close]
BLAST of SAM and SH3 domain-containing protein vs. Ensembl Zebrafish
Match: sash1a (SAM and SH3 domain containing 1a [Source:ZFIN;Acc:ZDB-GENE-030131-9960])

HSP 1 Score: 51.9878 bits (123), Expect = 2.682e-6
Identity = 40/148 (27.03%), Postives = 71/148 (47.97%), Query Frame = 1 
Query: 1423 VERKLQFEQIDFTDIPYSDQKGNSGIPLRLIERYAYELDRDIRDVALAMETSRSDKLLEHNRDVRSNMDAILSDQFLN-C----DGIKTDSLDSFLTSIGLPMYKGKIRIKCRNLQTFLLYSDEALIRNFNIHPS-HVRWIRREASFF 1848
            +E ++  E ID  + PYSD+ G  GIP  L++RY+ +  +  +DVA +++  R  +L + +R       AI S    + C      +   S+  +L SIG+PMY   +     +  + +   +EA  R+  +    H+R +  EA   
Sbjct: 1043 LEDRMHSEGIDLKEEPYSDKHGRCGIPQLLVQRYSEDFQQPAKDVASSLDQIRVKQLRKEHR------MAIPSGGLTDMCRKPVSLVHVSSVSDWLVSIGMPMYSAPLLAAGFDTLSRVSSLNEAQARDAGLKEEHHIRILLSEAQLV 1184
[Close]
BLAST of SAM and SH3 domain-containing protein vs. Ensembl Xenopus
Match: appbp2 (amyloid beta precursor protein (cytoplasmic tail) binding protein 2 [Source:Xenbase;Acc:XB-GENE-951373])

HSP 1 Score: 60.8474 bits (146), Expect = 6.755e-9
Identity = 40/104 (38.46%), Postives = 57/104 (54.81%), Query Frame = 1 
Query: 1423 VERKLQFEQIDFTDIPYSDQKGNSGIPLRLIERYAYELDRDIRDVALAMETSRSDKLLEHNRDVRSNMDAILSDQFLN-CDGIKT----DSLDSFLTSIGLPMY 1719
            +E KL  E+ID T+ PYSD+ G  GIP  L ERY+ +L +  +DVA  M+  R+  L++ +R       AILS      C    +     S+  +L S+GLPMY
Sbjct:  846 IENKLLSEKIDLTEEPYSDKHGRYGIPEALTERYSEDLGQPEKDVAATMDLIRAKHLMKLHR------MAILSGGLTEICRKPVSPRHISSVTDWLVSVGLPMY 943
[Close]
BLAST of SAM and SH3 domain-containing protein vs. Ensembl Mouse
Match: Sash1 (SAM and SH3 domain containing 1 [Source:MGI Symbol;Acc:MGI:1917347])

HSP 1 Score: 66.2402 bits (160), Expect = 1.052e-10
Identity = 42/101 (41.58%), Postives = 58/101 (57.43%), Query Frame = 1 
Query: 1423 VERKLQFEQIDFTDIPYSDQKGNSGIPLRLIERYAYELDRDIRDVALAMETSRSDKLL--EHNRDVRSNMDAILSDQFLNCDGIKTDSLDSFLTSIGLPMY 1719
             E KLQ E ID T+ PYSD+ G  GIP  L++RYA +L++  RDVA  M+  R  KLL  +H   + S     +  + L+   +   S+  +L SIGLPMY
Sbjct: 1080 TENKLQAEGIDLTEEPYSDKHGRCGIPEALVQRYAEDLEQPERDVATNMDQIRV-KLLRKQHRMAIPSGGLTEICRKPLSPGCVA--SMSDWLISIGLPMY 1177
[Close]
BLAST of SAM and SH3 domain-containing protein vs. UniProt/SwissProt
Match: sp|O94885|SASH1_HUMAN (SAM and SH3 domain-containing protein 1 OS=Homo sapiens OX=9606 GN=SASH1 PE=1 SV=3)

HSP 1 Score: 68.1662 bits (165), Expect = 1.865e-10
Identity = 41/104 (39.42%), Postives = 58/104 (55.77%), Query Frame = 1 
Query: 1423 VERKLQFEQIDFTDIPYSDQKGNSGIPLRLIERYAYELDRDIRDVALAMETSRSDKLL-EHNRDVRSNMDAILSDQFLNCDGIKTDSLDSFLTSIGLPMYKGKI 1731
             E KL  E ID T+ PYSD+ G  GIP  L++RYA +LD+  RDVA  M+  R  +L  +H   + S     +  + ++   I   S+  +L SIGLPMY G +
Sbjct: 1097 TENKLHAEGIDLTEEPYSDKHGRCGIPEALVQRYAEDLDQPERDVAANMDQIRVKQLRKQHRMAIPSGGLTEICRKPVSPGCIS--SVSDWLISIGLPMYAGTL 1198
[Close]
BLAST of SAM and SH3 domain-containing protein vs. UniProt/SwissProt
Match: sp|P59808|SASH1_MOUSE (SAM and SH3 domain-containing protein 1 OS=Mus musculus OX=10090 GN=Sash1 PE=1 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 7.299e-10
Identity = 42/101 (41.58%), Postives = 58/101 (57.43%), Query Frame = 1 
Query: 1423 VERKLQFEQIDFTDIPYSDQKGNSGIPLRLIERYAYELDRDIRDVALAMETSRSDKLL--EHNRDVRSNMDAILSDQFLNCDGIKTDSLDSFLTSIGLPMY 1719
             E KLQ E ID T+ PYSD+ G  GIP  L++RYA +L++  RDVA  M+  R  KLL  +H   + S     +  + L+   +   S+  +L SIGLPMY
Sbjct: 1080 TENKLQAEGIDLTEEPYSDKHGRCGIPEALVQRYAEDLEQPERDVATNMDQIRV-KLLRKQHRMAIPSGGLTEICRKPLSPGCVA--SMSDWLISIGLPMY 1177
[Close]
BLAST of SAM and SH3 domain-containing protein vs. TrEMBL
Match: A0A5J4NW38 (Uncharacterized protein OS=Paragonimus westermani OX=34504 GN=DEA37_0002565 PE=4 SV=1)

HSP 1 Score: 124.02 bits (310), Expect = 4.579e-27
Identity = 79/208 (37.98%), Postives = 105/208 (50.48%), Query Frame = 1 
Query: 1366 PLLQWKLPDPRESEKTRQSVERKLQFEQIDFTDIPYSDQKGNSGIPLRLIERYAYELDRDIRDVALAMETSRSDKLLEHNRDVRSNMDAILSDQFLNCDGIKTDSLDSFLTSIGLPMYKGKIRIKCRNLQT------------------FLLYSDEALIRNFNIHPSHVRWIRREASFFPIGFLSQIS----HVPPQRFRTTSDAKYF 1923
            PL  W+L            V  KLQ E ID T  PYS++ G + IPLRL +RYA EL  +   VA+A+E  R  KL E  R V S MD I S ++LNCDGIKT +++ FL S+GLPMY  +I+    N Q                    L  SD+ LI++     +H+RW+R EA+  P    +Q S    H+ P      S A+ F
Sbjct:  108 PLFLWRL------------VRVKLQSEGIDLTKKPYSNEIGQADIPLRLTQRYASELGLEWTTVAIALEAEREAKLREAGRPVISFMDDIQSQRYLNCDGIKTGTVEDFLISLGLPMYIDRIKQLPMNGQPSPDPSIHSPSGYPVGPVDILKMSDQDLIKHLGFTWNHIRWLRIEAAMIPSIMRAQASFGTTHMKPSGSHMNSLAREF 303
[Close]
BLAST of SAM and SH3 domain-containing protein vs. TrEMBL
Match: A0A0R3UID5 (Uncharacterized protein OS=Mesocestoides corti OX=53468 GN=MCOS_LOCUS7182 PE=4 SV=1)

HSP 1 Score: 117.087 bits (292), Expect = 1.246e-25
Identity = 65/156 (41.67%), Postives = 90/156 (57.69%), Query Frame = 1 
Query: 1423 VERKLQFEQIDFTDIPYSDQKGNSGIPLRLIERYAYELDRDIRDVALAMETSRSDKLLEHNRDVRSNMDAILSDQFLNCDGIKTDSLDSFLTSIGLPMYKGKIRIKCR--------NLQTFLLYSDEALIRNFNIHPSHVRWIRREASFFPIGFLS 1866
            + + L+ E+ID ++ PY++  G SGIPLRLIERYA E ++D   +AL +E  R   L +      SN++ I S ++LNC+GIKTD+L  FLT+IGLPMY   +    R        NL T    +D  +I +  I   HVR IR EA+  P   LS
Sbjct:   50 IVKWLRLERIDLSEPPYTNDAGGSGIPLRLIERYACEAEKDCTTIALTLERIREQILRKAQLPFASNLEEIHSRRYLNCNGIKTDNLGDFLTTIGLPMYAECLTTALRGNNAPIPANLSTL---TDAEMIFSLGIPVEHVRRIRAEAALIPRNHLS 202
[Close]
BLAST of SAM and SH3 domain-containing protein vs. TrEMBL
Match: A0A183MFF1 (Uncharacterized protein OS=Schistosoma margrebowiei OX=48269 GN=SMRZ_LOCUS14776 PE=4 SV=1)

HSP 1 Score: 122.479 bits (306), Expect = 1.734e-25
Identity = 73/168 (43.45%), Postives = 92/168 (54.76%), Query Frame = 1 
Query: 1414 RQSVERKLQFEQIDFTDIPYSDQKGNSGIPLRLIERYAYELDRDIRDVALAMETSRSDKLLEHNRDVRSNMDAILSDQFLNCDGIKTDSLDSFLTSIGLPMYKGKI-------------RIKCRNLQT---FLLYSDEALIRNFNIHPSHVRWIRREASFFPIGFLSQ 1869
            R  + +KL  E ID T  PYSD+ G + IPLRLI+RY++E + D+  VA+A+E  R   L E  R V S  + I S +FLNCDGIKT SL  FL +IGLPMY   I               KC  L T    L  S+  L   FN  P H++W+R+EAS  P   L Q
Sbjct:  136 RHILRKKLNTEHIDLTQPPYSDETGRANIPLRLIQRYSFETNLDLLTVAMALEAERDTNLREIRRPVISFNEEIRSQRFLNCDGIKTGSLQEFLITIGLPMYINHILHYNNITTNNNNHNHKCL-LMTPADLLNMSNCQLQEKFNFLPIHIQWLRQEASVIPWILLGQ 302
[Close]
BLAST of SAM and SH3 domain-containing protein vs. TrEMBL
Match: A0A5K3EXX5 (Uncharacterized protein OS=Mesocestoides corti OX=53468 PE=4 SV=1)

HSP 1 Score: 116.701 bits (291), Expect = 2.522e-25
Identity = 65/156 (41.67%), Postives = 90/156 (57.69%), Query Frame = 1 
Query: 1423 VERKLQFEQIDFTDIPYSDQKGNSGIPLRLIERYAYELDRDIRDVALAMETSRSDKLLEHNRDVRSNMDAILSDQFLNCDGIKTDSLDSFLTSIGLPMYKGKIRIKCR--------NLQTFLLYSDEALIRNFNIHPSHVRWIRREASFFPIGFLS 1866
            + + L+ E+ID ++ PY++  G SGIPLRLIERYA E ++D   +AL +E  R   L +      SN++ I S ++LNC+GIKTD+L  FLT+IGLPMY   +    R        NL T    +D  +I +  I   HVR IR EA+  P   LS
Sbjct:   71 IVKWLRLERIDLSEPPYTNDAGGSGIPLRLIERYACEAEKDCTTIALTLERIREQILRKAQLPFASNLEEIHSRRYLNCNGIKTDNLGDFLTTIGLPMYAECLTTALRGNNAPIPANLSTL---TDAEMIFSLGIPVEHVRRIRAEAALIPRNHLS 223
[Close]
BLAST of SAM and SH3 domain-containing protein vs. TrEMBL
Match: A0A419Q707 (Uncharacterized protein (Fragment) OS=Clonorchis sinensis OX=79923 GN=CSKR_4952s PE=4 SV=1)

HSP 1 Score: 123.25 bits (308), Expect = 2.607e-25
Identity = 67/164 (40.85%), Postives = 90/164 (54.88%), Query Frame = 1 
Query: 1417 QSVERKLQFEQIDFTDIPYSDQKGNSGIPLRLIERYAYELDRDIRDVALAMETSRSDKLLEHNRDVRSNMDAILSDQFLNCDGIKTDSLDSFLTSIGLPMYKGKIRIKCRNLQT------------FLLYSDEALIRNFNIHPSHVRWIRREASFFPIGFLSQI 1872
            Q++   LQ E ID T  PYS++ G + IPLRL +RYA+EL  D+  VA+ ME  R  +L E  R V S +D I S ++LNCDGIKT S++ FL  IGLPMY  +++    +                L  SD  LI        H+ W+RRE+S  P   LSQ+
Sbjct:  547 QTLRMCLQSEGIDLTQKPYSNEMGQANIPLRLTQRYAFELGWDLSTVAILMEAEREHRLREAGRPVVSFIDDIQSQRYLNCDGIKTGSIEDFLIGIGLPMYIDRVKRMSGSFSKSSQPEQSITPVQLLKMSDTELINEIGFTKGHLDWLRRESSVIPSVLLSQL 710
[Close]
BLAST of SAM and SH3 domain-containing protein vs. Ensembl Cavefish
Match: sash1a (SAM and SH3 domain containing 1 [Source:NCBI gene;Acc:103044068])

HSP 1 Score: 56.225 bits (134), Expect = 9.863e-8
Identity = 42/155 (27.10%), Postives = 69/155 (44.52%), Query Frame = 1 
Query: 1423 VERKLQFEQIDFTDIPYSDQKGNSGIPLRLIERYAYELDRDIRDVALAMETSRSDKLLEHNRDVRSNMDAILSDQFLN-C----DGIKTDSLDSFLTSIGLPMYKGKIRIKCRNLQTFLLYSDEALIRNFNIHPS-HVRWIRREASFFPIGFLSQ 1869
            +E +L  E ID T  PYSD+ G  GIP  L++RY+ +  + ++D+A  ++  R  +L + +R       AI S    + C      +   S+  +L SIG+PMY   +          L    E  +R   +    H+R +  EA        +Q
Sbjct: 1009 LEERLGSEGIDLTAEPYSDKHGRCGIPQALVQRYSEDFQQPVKDMASTLDQIRVKQLRKDHR------MAIPSGGLTDLCRKPTSPVHLSSVSDWLVSIGMPMYSDLLLAAGYETLGHLSALTETGVREAGLQEERHIRRLLSEARLVTASSATQ 1157
[Close]
BLAST of SAM and SH3 domain-containing protein vs. Ensembl Cavefish
Match: sash1a (SAM and SH3 domain containing 1 [Source:NCBI gene;Acc:103044068])

HSP 1 Score: 55.8398 bits (133), Expect = 1.295e-7
Identity = 42/155 (27.10%), Postives = 69/155 (44.52%), Query Frame = 1 
Query: 1423 VERKLQFEQIDFTDIPYSDQKGNSGIPLRLIERYAYELDRDIRDVALAMETSRSDKLLEHNRDVRSNMDAILSDQFLN-C----DGIKTDSLDSFLTSIGLPMYKGKIRIKCRNLQTFLLYSDEALIRNFNIHPS-HVRWIRREASFFPIGFLSQ 1869
            +E +L  E ID T  PYSD+ G  GIP  L++RY+ +  + ++D+A  ++  R  +L + +R       AI S    + C      +   S+  +L SIG+PMY   +          L    E  +R   +    H+R +  EA        +Q
Sbjct: 1209 LEERLGSEGIDLTAEPYSDKHGRCGIPQALVQRYSEDFQQPVKDMASTLDQIRVKQLRKDHR------MAIPSGGLTDLCRKPTSPVHLSSVSDWLVSIGMPMYSDLLLAAGYETLGHLSALTETGVREAGLQEERHIRRLLSEARLVTASSATQ 1357
[Close]
BLAST of SAM and SH3 domain-containing protein vs. Ensembl Cavefish
Match: sash1a (SAM and SH3 domain containing 1 [Source:NCBI gene;Acc:103044068])

HSP 1 Score: 55.8398 bits (133), Expect = 1.356e-7
Identity = 42/155 (27.10%), Postives = 69/155 (44.52%), Query Frame = 1 
Query: 1423 VERKLQFEQIDFTDIPYSDQKGNSGIPLRLIERYAYELDRDIRDVALAMETSRSDKLLEHNRDVRSNMDAILSDQFLN-C----DGIKTDSLDSFLTSIGLPMYKGKIRIKCRNLQTFLLYSDEALIRNFNIHPS-HVRWIRREASFFPIGFLSQ 1869
            +E +L  E ID T  PYSD+ G  GIP  L++RY+ +  + ++D+A  ++  R  +L + +R       AI S    + C      +   S+  +L SIG+PMY   +          L    E  +R   +    H+R +  EA        +Q
Sbjct: 1176 LEERLGSEGIDLTAEPYSDKHGRCGIPQALVQRYSEDFQQPVKDMASTLDQIRVKQLRKDHR------MAIPSGGLTDLCRKPTSPVHLSSVSDWLVSIGMPMYSDLLLAAGYETLGHLSALTETGVREAGLQEERHIRRLLSEARLVTASSATQ 1324
[Close]
BLAST of SAM and SH3 domain-containing protein vs. Ensembl Medaka
Match: sash1a (SAM and SH3 domain containing 1 [Source:NCBI gene;Acc:101164614])

HSP 1 Score: 65.0846 bits (157), Expect = 2.031e-10
Identity = 42/144 (29.17%), Postives = 72/144 (50.00%), Query Frame = 1 
Query: 1423 VERKLQFEQIDFTDIPYSDQKGNSGIPLRLIERYAYELDRDIRDVALAMETSRSDKLL-EHNRDVRSNMDAILSDQFLNCDGIKTDSLDSFLTSIGLPMYKGKIRIKCRNLQTFLLYSDEALIRNFNIHPS-HVRWIRREASFF 1848
            +E++LQ E ID T+ PYSD+ G  GIP  L++RY+ +L++ ++DVA  ++  R  +L  +H   + S     +  +  +   I T  +  +L SIGLPMY   +     N  +      E+ ++   +    H R +  EA   
Sbjct: 1076 LEQRLQTEGIDLTEDPYSDKHGRCGIPQLLVQRYSEDLEQPVKDVAPLLDQLRVKELRKQHRMAIPSGGLTEMCRKPASLGNIST--VSDWLISIGLPMYASSLSAAGVNTLSRAALLTESSVKEAGVRDERHARRLVSEARLI 1217
[Close]
BLAST of SAM and SH3 domain-containing protein vs. Planmine SMEST
Match: SMESG000011174.1 (SMESG000011174.1)

HSP 1 Score: 1130.55 bits (2923), Expect = 0.000e+0
Identity = 585/606 (96.53%), Postives = 587/606 (96.86%), Query Frame = 1 
Query:  121 MSFNENAGSSSLTNFIRSRVSRFGRFGLSKKYQRVLMTPLSDDQRKTVIDKLTKKGLQPQPEWNKQNKSEIPLPKKPDEFDRKKVTFESHSRNSVYFDSTSRLMEELLSSAPSSPTMSERSKFIRPGALNLNLDPGXXXXXXXXXXXXXXXXXGYYDTIPLLPECRDNSKEFFHSTNVPFKSFFPFPANESPFNQIPHYPDDRSRFVIGSEEPTTATNENIHVRRSRVQSLSHLLEKLQIKDLLPTFKIFGIEDLRAFTKLTEVMLDRMHITSTEVKTKILTALELLSETEDCPENEVFEKSKSIDPSTKSNSSHLQPQLDISDQGQMNQSVIRKPSSYHLSRSHRAARDMKGSSTRQPSGHRKHIXXXXXXXXXQKNRPYQTQISDLPHCKETTXXXXXXXXXXXVGSSGFFGQPLLQWKLPDPRESEKTRQSVERKLQFEQIDFTDIPYSDQKGNSGIPLRLIERYAYELDRDIRDVALAMETSRSDKLLEHNRDVRSNMDAILSDQFLNCDGIKTDSLDSFLTSIGLPMYKGKIRIKCRNLQTFLLYSDEALIRNFNIHPSHVRWIRREASFFPIGFLSQISHVPPQRFRTTSDAKYFALDRV 1938
            MSFNENAGSSSLTNFIRSRVSRFGRFGLSKKYQRVLMTPLSDDQRKTVIDKLTKKGLQPQPEWNKQNKSEIPLPKKPDEFDRKKVTFESHSRNSVYFDSTSRLMEELLSSAPSSPTMSERSKFIRPGALNLNLDPGQISDVSQSAPSSPS+DIGYYDTIPLLPECRDNSKEFFHSTNVPFKSFFPFPANESPFNQIPHYPDDRSRFVIGSEEPTTATNENIHVRRSRVQSLSHLLEKLQIKDLLPTFKIFGIEDLRAFTKLTEVMLDRM ITSTEVKTKILTALELLSETEDCPENE FEKSKSIDPSTKSNSSHLQPQLDI+DQGQMNQSVIRKPSSYHLSRSHRAARDMKGSSTRQPSGHRKHISSKRSGSSSQKNRPYQTQISDLPHC                 SSGFFGQPLLQWKLPDPRESEKTRQSVERKLQFEQIDFTDIPYSDQKGNSGIPLRLIERYAYELDRDIRDVALAMETSRSDKLLEHNRDVRSNMDAILSDQFLNCDGIKTDSLDSFLTSIGLPMYKGKIRIKCRNLQTFLLYSDEALIRNFNIHPSHVRWIRREASFFPIGFLSQISHVPPQRFRTTSDAKYFALDRV
Sbjct:    1 MSFNENAGSSSLTNFIRSRVSRFGRFGLSKKYQRVLMTPLSDDQRKTVIDKLTKKGLQPQPEWNKQNKSEIPLPKKPDEFDRKKVTFESHSRNSVYFDSTSRLMEELLSSAPSSPTMSERSKFIRPGALNLNLDPGQISDVSQSAPSSPSIDIGYYDTIPLLPECRDNSKEFFHSTNVPFKSFFPFPANESPFNQIPHYPDDRSRFVIGSEEPTTATNENIHVRRSRVQSLSHLLEKLQIKDLLPTFKIFGIEDLRAFTKLTEVMLDRMQITSTEVKTKILTALELLSETEDCPENEGFEKSKSIDPSTKSNSSHLQPQLDINDQGQMNQSVIRKPSSYHLSRSHRAARDMKGSSTRQPSGHRKHISSKRSGSSSQKNRPYQTQISDLPHC-----------------SSGFFGQPLLQWKLPDPRESEKTRQSVERKLQFEQIDFTDIPYSDQKGNSGIPLRLIERYAYELDRDIRDVALAMETSRSDKLLEHNRDVRSNMDAILSDQFLNCDGIKTDSLDSFLTSIGLPMYKGKIRIKCRNLQTFLLYSDEALIRNFNIHPSHVRWIRREASFFPIGFLSQISHVPPQRFRTTSDAKYFALDRV 589
The following BLAST results are available for this feature:
BLAST of SAM and SH3 domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2
Match NameE-valueIdentityDescription
SASH11.272e-1139.42SAM and SH3 domain containing 1 [Source:HGNC Symbo... [more]
SASH13.883e-1139.42SAM and SH3 domain containing 1 [Source:HGNC Symbo... [more]
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BLAST of SAM and SH3 domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SAM and SH3 domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SAM and SH3 domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 4
Match NameE-valueIdentityDescription
sash1b1.356e-841.94SAM and SH3 domain containing 1b [Source:ZFIN;Acc:... [more]
sash1b1.415e-841.94SAM and SH3 domain containing 1b [Source:ZFIN;Acc:... [more]
sash1b1.543e-841.94SAM and SH3 domain containing 1b [Source:ZFIN;Acc:... [more]
sash1a2.682e-627.03SAM and SH3 domain containing 1a [Source:ZFIN;Acc:... [more]
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BLAST of SAM and SH3 domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
appbp26.755e-938.46amyloid beta precursor protein (cytoplasmic tail) ... [more]
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BLAST of SAM and SH3 domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Sash11.052e-1041.58SAM and SH3 domain containing 1 [Source:MGI Symbol... [more]
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BLAST of SAM and SH3 domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 2
Match NameE-valueIdentityDescription
sp|O94885|SASH1_HUMAN1.865e-1039.42SAM and SH3 domain-containing protein 1 OS=Homo sa... [more]
sp|P59808|SASH1_MOUSE7.299e-1041.58SAM and SH3 domain-containing protein 1 OS=Mus mus... [more]
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BLAST of SAM and SH3 domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A5J4NW384.579e-2737.98Uncharacterized protein OS=Paragonimus westermani ... [more]
A0A0R3UID51.246e-2541.67Uncharacterized protein OS=Mesocestoides corti OX=... [more]
A0A183MFF11.734e-2543.45Uncharacterized protein OS=Schistosoma margrebowie... [more]
A0A5K3EXX52.522e-2541.67Uncharacterized protein OS=Mesocestoides corti OX=... [more]
A0A419Q7072.607e-2540.85Uncharacterized protein (Fragment) OS=Clonorchis s... [more]
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BLAST of SAM and SH3 domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
sash1a9.863e-827.10SAM and SH3 domain containing 1 [Source:NCBI gene;... [more]
sash1a1.295e-727.10SAM and SH3 domain containing 1 [Source:NCBI gene;... [more]
sash1a1.356e-727.10SAM and SH3 domain containing 1 [Source:NCBI gene;... [more]
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BLAST of SAM and SH3 domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SAM and SH3 domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SAM and SH3 domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SAM and SH3 domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
sash1a2.031e-1029.17SAM and SH3 domain containing 1 [Source:NCBI gene;... [more]
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BLAST of SAM and SH3 domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000011174.10.000e+096.53SMESG000011174.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30020445 ID=SMED30020445|Name=SAM and SH3 domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=2009bp
TGTGAAATCAATTCGAGATTTTCAATTATAATCAGCGACTGCGGTGCTAT
AGCTTCATTTAATATTTCGTTATGTTGGTGCTTGATTATTATTGTATAAA
TACTGCCGGTTGCTGTTGCTATGAGTTTTAATGAAAATGCCGGATCGTCG
AGTCTCACCAACTTCATCCGGAGCAGGGTTTCCAGGTTCGGGAGGTTTGG
ATTATCTAAAAAATATCAGCGAGTCCTAATGACACCCTTGTCGGATGACC
AAAGAAAAACAGTCATTGACAAATTAACCAAAAAAGGTTTACAGCCTCAA
CCTGAATGGAATAAACAGAACAAGTCAGAGATTCCACTTCCAAAGAAACC
AGATGAGTTTGATAGAAAAAAAGTTACATTTGAAAGTCATTCGAGAAATA
GTGTTTATTTTGATTCGACATCTAGATTAATGGAAGAGTTATTATCTTCT
GCTCCAAGCAGTCCAACGATGAGTGAAAGATCAAAATTTATACGACCAGG
AGCTTTGAATTTAAATCTTGACCCTGGTCAAATTTCTGACGTGAGCCAAT
CTGCTCCAAGCAGTCCAAGTGTTGACATTGGTTATTACGACACAATTCCA
CTTCTTCCTGAATGTAGAGATAACTCAAAAGAATTTTTCCATTCAACTAA
TGTTCCTTTTAAGTCTTTCTTTCCATTTCCAGCAAATGAATCACCATTCA
ATCAAATACCTCATTATCCCGATGACCGTTCACGATTTGTGATTGGCTCG
GAAGAACCAACAACGGCTACCAATGAAAATATTCATGTACGAAGAAGCAG
AGTTCAGTCTTTGTCTCACTTGCTTGAAAAGCTTCAAATTAAAGATTTGT
TACCGACTTTTAAAATATTTGGAATAGAAGATCTCAGGGCTTTCACAAAA
CTTACCGAAGTCATGCTGGATCGAATGCATATAACCAGCACGGAAGTAAA
AACAAAAATACTAACCGCATTGGAACTGCTTTCAGAAACAGAAGACTGTC
CAGAAAATGAGGTGTTCGAAAAGTCAAAATCAATTGATCCCTCCACAAAA
TCCAACTCGAGCCATTTACAACCTCAGTTGGATATTAGTGACCAAGGACA
AATGAATCAATCTGTCATAAGGAAGCCCAGCAGTTATCATTTGAGTAGAT
CGCACAGGGCTGCAAGGGACATGAAAGGCAGCTCTACTAGACAGCCTAGT
GGACACAGAAAGCATATTTCCTCAAAGAGATCTGGATCAAGTTCGCAGAA
AAATAGACCATATCAAACTCAGATTTCTGATTTACCTCATTGTAAGGAAA
CAACCATCATCCATCAGCATATTCATCATCACCATCATGTAGGCAGCTCT
GGATTTTTTGGTCAACCTTTACTTCAATGGAAGCTTCCTGATCCAAGAGA
ATCTGAAAAGACAAGACAATCGGTAGAAAGAAAACTCCAATTCGAACAAA
TAGATTTCACTGATATTCCCTATTCAGACCAAAAAGGGAACTCGGGCATA
CCACTGCGTTTAATTGAAAGATATGCCTATGAATTAGACAGAGACATCAG
AGATGTTGCATTGGCAATGGAAACGAGTCGAAGTGACAAGTTGTTAGAGC
ATAATCGTGATGTGCGGTCAAACATGGACGCAATTCTGTCAGATCAGTTC
CTCAACTGTGACGGCATTAAAACAGACAGTCTTGACAGTTTCCTGACATC
AATCGGTTTGCCGATGTACAAAGGGAAAATCCGTATTAAATGTCGAAATC
TTCAAACTTTTTTGTTATATTCTGATGAAGCCTTGATAAGAAATTTCAAC
ATCCACCCGTCCCATGTCCGGTGGATCCGACGAGAGGCTTCTTTTTTTCC
AATTGGATTTCTTAGTCAAATATCCCATGTTCCACCGCAGAGATTTAGAA
CGACGTCCGACGCAAAGTATTTTGCTCTTGATCGAGTTTAATTAAATATG
TTGATCTTCATGTTACATTTTGAAGTATTGATACTGATGTATAAATTGTT
TTGCTAGAG
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protein sequence of SMED30020445-orf-1

>SMED30020445-orf-1 ID=SMED30020445-orf-1|Name=SMED30020445-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=607bp
MSFNENAGSSSLTNFIRSRVSRFGRFGLSKKYQRVLMTPLSDDQRKTVID
KLTKKGLQPQPEWNKQNKSEIPLPKKPDEFDRKKVTFESHSRNSVYFDST
SRLMEELLSSAPSSPTMSERSKFIRPGALNLNLDPGQISDVSQSAPSSPS
VDIGYYDTIPLLPECRDNSKEFFHSTNVPFKSFFPFPANESPFNQIPHYP
DDRSRFVIGSEEPTTATNENIHVRRSRVQSLSHLLEKLQIKDLLPTFKIF
GIEDLRAFTKLTEVMLDRMHITSTEVKTKILTALELLSETEDCPENEVFE
KSKSIDPSTKSNSSHLQPQLDISDQGQMNQSVIRKPSSYHLSRSHRAARD
MKGSSTRQPSGHRKHISSKRSGSSSQKNRPYQTQISDLPHCKETTIIHQH
IHHHHHVGSSGFFGQPLLQWKLPDPRESEKTRQSVERKLQFEQIDFTDIP
YSDQKGNSGIPLRLIERYAYELDRDIRDVALAMETSRSDKLLEHNRDVRS
NMDAILSDQFLNCDGIKTDSLDSFLTSIGLPMYKGKIRIKCRNLQTFLLY
SDEALIRNFNIHPSHVRWIRREASFFPIGFLSQISHVPPQRFRTTSDAKY
FALDRV*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000101muscle cell
Vocabulary: INTERPRO
TermDefinition
IPR013761SAM/pointed_sf
Vocabulary: biological process
TermDefinition
GO:0000209protein polyubiquitination
GO:0010595positive regulation of endothelial cell migration
GO:0031666positive regulation of lipopolysaccharide-mediated signaling pathway
GO:0043507positive regulation of JUN kinase activity
GO:0045766positive regulation of angiogenesis
GO:1900044regulation of protein K63-linked ubiquitination
GO:1900745positive regulation of p38MAPK cascade
GO:1901224positive regulation of NIK/NF-kappaB signaling
GO:1902498regulation of protein autoubiquitination
Vocabulary: molecular function
TermDefinition
GO:0008022protein C-terminus binding
GO:0019901protein kinase binding
GO:0031435mitogen-activated protein kinase kinase kinase binding
GO:0032947protein complex scaffold
Vocabulary: cellular component
TermDefinition
GO:0043234protein complex
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013761Sterile alpha motif/pointed domain superfamilyGENE3DG3DSA:1.10.150.50coord: 220..292
e-value: 3.0E-7
score: 32.3
IPR013761Sterile alpha motif/pointed domain superfamilySUPERFAMILYSSF47769SAM/Pointed domaincoord: 223..290
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 291..316
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 53..77
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 335..382
NoneNo IPR availablePANTHERPTHR12301SAM-DOMAIN, SH3 AND NUCLEAR LOCALIZATION SIGNALS PROTEIN RELATEDcoord: 418..535
NoneNo IPR availablePANTHERPTHR12301SAM-DOMAIN, SH3 AND NUCLEAR LOCALIZATION SIGNALS PROTEIN RELATEDcoord: 219..298
NoneNo IPR availablePANTHERPTHR12301:SF3SAM AND SH3 DOMAIN-CONTAINING PROTEIN 1coord: 219..298
NoneNo IPR availablePANTHERPTHR12301:SF3SAM AND SH3 DOMAIN-CONTAINING PROTEIN 1coord: 418..535