Oxalate:formate antiporter

Overview
NameOxalate:formate antiporter
Smed IDSMED30020433
Length (bp)1400
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Oxalate:formate antiporter (SMED30020433) t-SNE clustered cells

Violin plots show distribution of expression levels for Oxalate:formate antiporter (SMED30020433) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Oxalate:formate antiporter (SMED30020433) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Oxalate:formate antiporter (SMED30020433) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30020433

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
gutSMED30020433h1SMcG0017153 dd_Smed_v4_4769_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30020433h1SMcG0017153 dd_Smed_v4_4769_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Oxalate:formate antiporter vs. Ensembl Celegans
Match: F10G7.5 (pep chromosome:WBcel235:II:4693414:4696408:1 gene:WBGene00017369.1 transcript:F10G7.5a.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:F10G7.5)

HSP 1 Score: 65.0846 bits (157), Expect = 4.293e-11
Identity = 50/170 (29.41%), Postives = 88/170 (51.76%), Query Frame = 3 
Query:  516 SAEIQMKPIKLMFQ---KDFWVLWLSYTFNSCVAILVLSLHKVFGETLSIIDINLTIASAIGAGFNGIGRIAWGFLIDKFSYKTIMTILTLSMSLMIGTFYLCSLIPNVHQKFIMFLIWVIILHFLLSGNFSIYPVICSRTFGQENSVVNYGVLFTAYALAGLITSSCTT 1016
            ++EI+++  ++  Q     F VL+LS T N+    L   L+K +G+     D  L++  +I + FN   R+ WG + D  SY+  M+I+    +++  +  L  ++ N     ++FL  V ++   + G FSI+P I  + F + N  V YG L  A ++AGLI    +T
Sbjct:  235 NSEIRVEQARVWPQLRTTTFLVLFLSLTCNAVWVQLTSGLYKAYGQQFIDSDFFLSLIGSISSIFNAGSRVFWGAIADTTSYQFSMSIVCSLGAVLAFSMPLVKMVSND----VLFLATVCLMFSCIGGTFSIFPYITHKCFSKANFSVMYGFLQCAVSVAGLIAGLIST 400
[Close]
BLAST of Oxalate:formate antiporter vs. Ensembl Celegans
Match: F10G7.5 (pep chromosome:WBcel235:II:4693542:4696408:1 gene:WBGene00017369.1 transcript:F10G7.5a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:F10G7.5)

HSP 1 Score: 65.0846 bits (157), Expect = 4.293e-11
Identity = 50/170 (29.41%), Postives = 88/170 (51.76%), Query Frame = 3 
Query:  516 SAEIQMKPIKLMFQ---KDFWVLWLSYTFNSCVAILVLSLHKVFGETLSIIDINLTIASAIGAGFNGIGRIAWGFLIDKFSYKTIMTILTLSMSLMIGTFYLCSLIPNVHQKFIMFLIWVIILHFLLSGNFSIYPVICSRTFGQENSVVNYGVLFTAYALAGLITSSCTT 1016
            ++EI+++  ++  Q     F VL+LS T N+    L   L+K +G+     D  L++  +I + FN   R+ WG + D  SY+  M+I+    +++  +  L  ++ N     ++FL  V ++   + G FSI+P I  + F + N  V YG L  A ++AGLI    +T
Sbjct:  235 NSEIRVEQARVWPQLRTTTFLVLFLSLTCNAVWVQLTSGLYKAYGQQFIDSDFFLSLIGSISSIFNAGSRVFWGAIADTTSYQFSMSIVCSLGAVLAFSMPLVKMVSND----VLFLATVCLMFSCIGGTFSIFPYITHKCFSKANFSVMYGFLQCAVSVAGLIAGLIST 400
[Close]
BLAST of Oxalate:formate antiporter vs. Ensembl Celegans
Match: F10G7.5 (pep chromosome:WBcel235:II:4693414:4696408:1 gene:WBGene00017369.1 transcript:F10G7.5b.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:F10G7.5)

HSP 1 Score: 65.0846 bits (157), Expect = 4.803e-11
Identity = 50/170 (29.41%), Postives = 88/170 (51.76%), Query Frame = 3 
Query:  516 SAEIQMKPIKLMFQ---KDFWVLWLSYTFNSCVAILVLSLHKVFGETLSIIDINLTIASAIGAGFNGIGRIAWGFLIDKFSYKTIMTILTLSMSLMIGTFYLCSLIPNVHQKFIMFLIWVIILHFLLSGNFSIYPVICSRTFGQENSVVNYGVLFTAYALAGLITSSCTT 1016
            ++EI+++  ++  Q     F VL+LS T N+    L   L+K +G+     D  L++  +I + FN   R+ WG + D  SY+  M+I+    +++  +  L  ++ N     ++FL  V ++   + G FSI+P I  + F + N  V YG L  A ++AGLI    +T
Sbjct:  249 NSEIRVEQARVWPQLRTTTFLVLFLSLTCNAVWVQLTSGLYKAYGQQFIDSDFFLSLIGSISSIFNAGSRVFWGAIADTTSYQFSMSIVCSLGAVLAFSMPLVKMVSND----VLFLATVCLMFSCIGGTFSIFPYITHKCFSKANFSVMYGFLQCAVSVAGLIAGLIST 414
[Close]
BLAST of Oxalate:formate antiporter vs. Ensembl Celegans
Match: Y59A8B.21 (pep chromosome:WBcel235:V:17986260:17994546:1 gene:WBGene00013353.1 transcript:Y59A8B.21.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Y59A8B.21)

HSP 1 Score: 56.9954 bits (136), Expect = 1.496e-8
Identity = 37/165 (22.42%), Postives = 79/165 (47.88%), Query Frame = 3 
Query:  513 DSAEIQMKPIKLMFQKDFWVLWLSYTFNSCVAILVLSLHKVFGETLSIIDINLTIASAIGAGFNGIGRIAWGFLIDKFSYKTIMTILTLSMSLMIGTFYLCSLIPNVHQKFIMFLIWVIILHFLLSGNFSIYPVICSRTFGQENSVVNYGVLFTAYALAGLITSS 1007
            +   IQM   +++    F+ L+ S    S  A +  +L+K +GE+    D+ + +A +I +  N I RI WG+L D+ S++     + LS +  + + +L ++        + FL W++     +    +++     + FG  +   NYG L  +   +G++ ++
Sbjct:  259 EDTTIQMTSSEMLKSPTFYCLFASLFCCSFYANMFYNLYKTYGESFIEDDMFMAMAFSIASVANAIARIGWGYLTDRTSFQ-----IALSTATCLASVFLLTMPMTRELGKMAFLFWLVGTFICMGATHALFITATVKCFGNRHKANNYGYLILSTTTSGILLAA 418
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BLAST of Oxalate:formate antiporter vs. UniProt/SwissProt
Match: sp|Q51330|OXLT_OXAFO (Oxalate:formate antiporter OS=Oxalobacter formigenes OX=847 GN=oxlT PE=1 SV=3)

HSP 1 Score: 56.6102 bits (135), Expect = 2.501e-7
Identity = 53/214 (24.77%), Postives = 95/214 (44.39%), Query Frame = 3 
Query:  543 KLMFQKDFWVLWLSYTFNSCVAILVLSLHKVFGETLSIIDINLTIASAIGAGFNGIGRIAWGFLIDKFS-YKTIMTILTLSMSLMIGTFYLCSLIPNVHQ-KFIMFLIWVIILHFLLSGNFSIYPVICSRTFGQENSVVNYGVLFTAYALAGLITSSCTTVIFKMTKNHNKLNENTKSNIYLIPFLVGSALSLLSAIVTRMYRG-RNILRSRPE 1175
            +++    FWVLW ++   +   +L+++    +G +L +    LTI  +I   FNG  R  WGF+ DK   YKT+  +  ++  ++       +L P +     + F+  + I  F   G+++++P   S  FG   S  NYG  + A A A +        I            NT   I  I   +  A +L + ++ RM R  + +++  PE
Sbjct:  215 EMLRTPQFWVLWTAFFSVNFGGLLLVANSVPYGRSLGLAAGVLTIGVSIQNLFNGGCRPFWGFVSDKIGRYKTMSVVFGINAVVL-------ALFPTIAALGDVAFIAMLAIAFFTWGGSYALFPSTNSDIFGTAYSARNYGFFWAAKATASIFGGGLGAAI------ATNFGWNTAFLITAITSFI--AFALATFVIPRMGRPVKKMVKLSPE 413
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BLAST of Oxalate:formate antiporter vs. UniProt/SwissProt
Match: sp|A0LNN5|SFMCT_SYNFM (L-lactate transporter OS=Syntrophobacter fumaroxidans (strain DSM 10017 / MPOB) OX=335543 GN=Sfum_3364 PE=1 SV=1)

HSP 1 Score: 53.1434 bits (126), Expect = 2.862e-6
Identity = 55/201 (27.36%), Postives = 83/201 (41.29%), Query Frame = 3 
Query:  561 DFWVLWLSYTFNSCVAILVLSLHKVFGETLSIIDINLTIASAIGA----GF-NGIGRIAWGFLIDKFSYKTIMTIL-TLSMSLMIGTFYLCSLIPNVHQKFIMFLIWVIILHFLLSGNFSIYPVICSRTFGQENSVVNYGVLFTAYALAGLITSSCTTVIFKMTKNHNKLNENTKSNIYLIPFLVGSALSLLSAIVTRMYR 1145
             FW+L+L+Y   S   ++V+     FG      D  LT  +A GA     F N   RI  G+ +DK   +     L  L  + MI  F L   +  +        I  I++ +     F+++P  C + +G      NYG+LFTA  LAG          F        L + T +  Y +PFL  +AL  L   +  M +
Sbjct:  229 KFWLLYLAYFCGSFAGLMVIGHLAGFGR-----DAGLTAMAAAGAVSSLAFSNAATRILSGWFVDKIGIRVYFAALFALQTAAMIAIFQLGGSVVGLS-------IVAIVIGWNYGAMFTLFPATCLQFYGPTAQGSNYGLLFTACGLAG----------FAGPWVGGWLKDTTGT--YYLPFLCAAALCALGTAIVFMTK 405
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BLAST of Oxalate:formate antiporter vs. TrEMBL
Match: A0A0H3YF10 (Slc16a-10 OS=Schmidtea mediterranea OX=79327 GN=slc16a-10 PE=2 SV=1)

HSP 1 Score: 154.451 bits (389), Expect = 4.256e-38
Identity = 72/169 (42.60%), Postives = 115/169 (68.05%), Query Frame = 3 
Query:  513 DSAEIQMKPIKLMFQKDFWVLWLSYTFNSCVAILVLSLHKVFGETLSIIDINLTIASAIGAGFNGIGRIAWGFLIDKFSYKTIMTILTLSMSLMIGTFYLCSLI-----PNVHQKFIMFLIWVIILHFLLSGNFSIYPVICSRTFGQENSVVNYGVLFTAYALAGLITS 1004
            ++ +I++KP +L+F  +FW++W++Y  NS  ++L + L K++G+ L I D+ L IA+  G  F+  GRI WG+L D+F YK ++   TL  ++M  TFY+CS +     PN + +F M+LIWVII+ FL+SGNFSI+P IC++ FG ++   N+GVL+T Y+   +IT 
Sbjct:  228 ETTKIELKPHQLIFYVNFWIIWITYALNSAFSLLFILLQKMWGKYLEISDLRLLIATCTGGIFSIFGRIIWGYLTDRFGYKIVIWTSTLLFAIMFSTFYICSEVGKLSTPNGNNRFAMYLIWVIIMFFLISGNFSIFPAICAKVFGIQHFTGNFGVLYTGYSFTAVITP 396
[Close]
BLAST of Oxalate:formate antiporter vs. TrEMBL
Match: A0A0H3YF06 (Slc16a-5 (Fragment) OS=Schmidtea mediterranea OX=79327 GN=slc16a-5 PE=2 SV=1)

HSP 1 Score: 122.094 bits (305), Expect = 2.037e-26
Identity = 70/201 (34.83%), Postives = 117/201 (58.21%), Query Frame = 3 
Query:  543 KLMFQKDFWVLWLSYTFNSCVAILVLSLHKVFGETLSIIDINLTIASAIGAGFNGIGRIAWGFLIDKFSYKTIMTILTLSMSLMIGTFYLCSLIPNVHQKFIMFLIWVIILHFLLSGNFSIYPVICSRTFGQENSVVNYGVLFTAYALAGLITSSCTTVIFKMTKNHNKLNENTKSNIYLIPFLVGSALSLLSAIVTRMYR 1145
            K + Q+DFW+LW  + F   + +   SL+K FG+     D  L +  ++ + FN +GRI WG ++D+FSYK  +  + ++ ++++GTFYLC L+PN     +MFLIWV  +   + G F++ P I ++ FG +  V  YG+LF+A A AG I  + TT IF      +K+N       Y   F +G+ L+ ++ I+T +++
Sbjct:  272 KAIKQRDFWILWFIFMFCGTINVYATSLYKTFGQLFITSDYYLALVGSVASIFNAMGRIFWGQVMDRFSYKVAIRSIIINSTILMGTFYLCHLVPNSIGARVMFLIWVCGIFANICGIFAVMPAITAKLFGLKKVVTIYGILFSANAFAG-IFGALTTSIF-----LSKIN-------YFGIFGIGAGLTTIALILTFLHK 459
[Close]
BLAST of Oxalate:formate antiporter vs. TrEMBL
Match: A0A1I8HL50 (MFS domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 115.931 bits (289), Expect = 2.458e-24
Identity = 68/211 (32.23%), Postives = 116/211 (54.98%), Query Frame = 3 
Query:  516 SAEIQMKPIKLMFQKDFWVLWLSYTFNSCVAILVLSLHKVFGETLSIIDINLTIASAIGAGFNGIGRIAWGFLIDKFSYKTIMTILTLSMSLMIGTFYLCSLIPNVHQKFIMFLIWVIILHFLLSGNFSIYPVICSRTFGQENSVVNYGVLFTAYALAGLITSSCTTVIFKMTKNHNKLNENTKSNI-YLIPFLVGSALSLLSAIVTRMYR 1145
              E+   P +++ +  FW+LW ++  NS   + + + +K FG +  + D  L I  ++ A FN   R+ WG L D+ +Y+ +M +L+  M++++ TF L S  P        +L+WV  +  L +GNF+++P  C++ +GQ N  VNYG+LFTA A +G+I +  TTV+              K  I +L  FL+G+A S L A+ T ++ 
Sbjct:  263 GGELDSTPGQMVRKPQFWLLWATFLVNSQSVVYINTQYKSFGLSKGLNDHYLAIVGSLAAAFNAASRVMWGHLHDRVAYRALMFVLSFGMAVLMSTFTL-SGAP------WYYLLWVCAMFSLFAGNFALFPAACAKLYGQTNLTVNYGLLFTANATSGIIGALLTTVL--------------KDAIGWLGLFLLGAAFSTLGAVFTLLFE 452
[Close]
BLAST of Oxalate:formate antiporter vs. TrEMBL
Match: A0A267H5U2 (MFS domain-containing protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig000477g2 PE=4 SV=1)

HSP 1 Score: 115.931 bits (289), Expect = 2.557e-24
Identity = 68/211 (32.23%), Postives = 116/211 (54.98%), Query Frame = 3 
Query:  516 SAEIQMKPIKLMFQKDFWVLWLSYTFNSCVAILVLSLHKVFGETLSIIDINLTIASAIGAGFNGIGRIAWGFLIDKFSYKTIMTILTLSMSLMIGTFYLCSLIPNVHQKFIMFLIWVIILHFLLSGNFSIYPVICSRTFGQENSVVNYGVLFTAYALAGLITSSCTTVIFKMTKNHNKLNENTKSNI-YLIPFLVGSALSLLSAIVTRMYR 1145
              E+   P +++ +  FW+LW ++  NS   + + + +K FG +  + D  L I  ++ A FN   R+ WG L D+ +Y+ +M +L+  M++++ TF L S  P        +L+WV  +  L +GNF+++P  C++ +GQ N  VNYG+LFTA A +G+I +  TTV+              K  I +L  FL+G+A S L A+ T ++ 
Sbjct:  265 GGELDSTPGQMVRKPQFWLLWATFLVNSQSVVYINTQYKSFGLSKGLNDHYLAIVGSLAAAFNAASRVMWGHLHDRVAYRALMFVLSFGMAVLMSTFTL-SGAP------WYYLLWVCAMFSLFAGNFALFPAACAKLYGQTNLTVNYGLLFTANATSGIIGALLTTVL--------------KDAIGWLGLFLLGAAFSTLGAVFTLLFE 454
[Close]
BLAST of Oxalate:formate antiporter vs. TrEMBL
Match: A0A267DK45 (MFS domain-containing protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig000477g4 PE=4 SV=1)

HSP 1 Score: 114.775 bits (286), Expect = 7.340e-24
Identity = 68/211 (32.23%), Postives = 115/211 (54.50%), Query Frame = 3 
Query:  516 SAEIQMKPIKLMFQKDFWVLWLSYTFNSCVAILVLSLHKVFGETLSIIDINLTIASAIGAGFNGIGRIAWGFLIDKFSYKTIMTILTLSMSLMIGTFYLCSLIPNVHQKFIMFLIWVIILHFLLSGNFSIYPVICSRTFGQENSVVNYGVLFTAYALAGLITSSCTTVIFKMTKNHNKLNENTKSNI-YLIPFLVGSALSLLSAIVTRMYR 1145
              E    P +++ +  FW+LW ++  NS   + + + +K FG +  + D  L I  ++ A FN   R+ WG L D+ +Y+ +M +L+  M++++ TF L S  P        +L+WV  +  L +GNF+++P  C++ +GQ N  VNYG+LFTA A +G+I +  TTV+              K  I +L  FL+G+A S L A+ T ++ 
Sbjct:  270 GGESDSTPGQMVRKPQFWLLWATFLVNSQSVVYINTQYKSFGLSKGLNDHYLAIVGSLAAAFNAASRVMWGHLHDRVAYRALMFVLSFGMAVLMSTFTL-SGAP------WYYLLWVCAMFSLFAGNFALFPAACAKLYGQTNLTVNYGLLFTANATSGIIGALLTTVL--------------KDAIGWLGLFLLGAAFSTLGAVFTLLFE 459
[Close]
BLAST of Oxalate:formate antiporter vs. Planmine SMEST
Match: SMESG000061390.1 (SMESG000061390.1)

HSP 1 Score: 442.965 bits (1138), Expect = 4.150e-153
Identity = 219/221 (99.10%), Postives = 220/221 (99.55%), Query Frame = 3 
Query:  513 DSAEIQMKPIKLMFQKDFWVLWLSYTFNSCVAILVLSLHKVFGETLSIIDINLTIASAIGAGFNGIGRIAWGFLIDKFSYKTIMTILTLSMSLMIGTFYLCSLIPNVHQKFIMFLIWVIILHFLLSGNFSIYPVICSRTFGQENSVVNYGVLFTAYALAGLITSSCTTVIFKMTKNHNKLNENTKSNIYLIPFLVGSALSLLSAIVTRMYRGRNILRSRPE 1175
            DSAEIQMKPIKLMFQKDFWVLWLSYTFNSCVAILVLSLHKVFGETLSIIDINLTIASAIGAGFNGIGRIAWGFLIDKFSYKTIMTILTLSMSLMIGTFYLCSLIPNVHQKFIMFLIWVIILHFLLSGNFSIYPVICSRTFGQENSVVNYGVLFTAYALAGLITSSCTTVIFKMTKNHNKLNENTKS IYLIPFLVGSALSLLSAIVTRMY+GRNILRSRPE
Sbjct:  229 DSAEIQMKPIKLMFQKDFWVLWLSYTFNSCVAILVLSLHKVFGETLSIIDINLTIASAIGAGFNGIGRIAWGFLIDKFSYKTIMTILTLSMSLMIGTFYLCSLIPNVHQKFIMFLIWVIILHFLLSGNFSIYPVICSRTFGQENSVVNYGVLFTAYALAGLITSSCTTVIFKMTKNHNKLNENTKSYIYLIPFLVGSALSLLSAIVTRMYQGRNILRSRPE 449
[Close]
BLAST of Oxalate:formate antiporter vs. Planmine SMEST
Match: SMESG000061390.1 (SMESG000061390.1)

HSP 1 Score: 429.869 bits (1104), Expect = 1.647e-151
Identity = 213/215 (99.07%), Postives = 214/215 (99.53%), Query Frame = 3 
Query:  531 MKPIKLMFQKDFWVLWLSYTFNSCVAILVLSLHKVFGETLSIIDINLTIASAIGAGFNGIGRIAWGFLIDKFSYKTIMTILTLSMSLMIGTFYLCSLIPNVHQKFIMFLIWVIILHFLLSGNFSIYPVICSRTFGQENSVVNYGVLFTAYALAGLITSSCTTVIFKMTKNHNKLNENTKSNIYLIPFLVGSALSLLSAIVTRMYRGRNILRSRPE 1175
            MKPIKLMFQKDFWVLWLSYTFNSCVAILVLSLHKVFGETLSIIDINLTIASAIGAGFNGIGRIAWGFLIDKFSYKTIMTILTLSMSLMIGTFYLCSLIPNVHQKFIMFLIWVIILHFLLSGNFSIYPVICSRTFGQENSVVNYGVLFTAYALAGLITSSCTTVIFKMTKNHNKLNENTKS IYLIPFLVGSALSLLSAIVTRMY+GRNILRSRPE
Sbjct:    1 MKPIKLMFQKDFWVLWLSYTFNSCVAILVLSLHKVFGETLSIIDINLTIASAIGAGFNGIGRIAWGFLIDKFSYKTIMTILTLSMSLMIGTFYLCSLIPNVHQKFIMFLIWVIILHFLLSGNFSIYPVICSRTFGQENSVVNYGVLFTAYALAGLITSSCTTVIFKMTKNHNKLNENTKSYIYLIPFLVGSALSLLSAIVTRMYQGRNILRSRPE 215
[Close]
BLAST of Oxalate:formate antiporter vs. Planmine SMEST
Match: SMESG000061390.1 (SMESG000061390.1)

HSP 1 Score: 417.927 bits (1073), Expect = 6.460e-147
Identity = 207/209 (99.04%), Postives = 208/209 (99.52%), Query Frame = 3 
Query:  549 MFQKDFWVLWLSYTFNSCVAILVLSLHKVFGETLSIIDINLTIASAIGAGFNGIGRIAWGFLIDKFSYKTIMTILTLSMSLMIGTFYLCSLIPNVHQKFIMFLIWVIILHFLLSGNFSIYPVICSRTFGQENSVVNYGVLFTAYALAGLITSSCTTVIFKMTKNHNKLNENTKSNIYLIPFLVGSALSLLSAIVTRMYRGRNILRSRPE 1175
            MFQKDFWVLWLSYTFNSCVAILVLSLHKVFGETLSIIDINLTIASAIGAGFNGIGRIAWGFLIDKFSYKTIMTILTLSMSLMIGTFYLCSLIPNVHQKFIMFLIWVIILHFLLSGNFSIYPVICSRTFGQENSVVNYGVLFTAYALAGLITSSCTTVIFKMTKNHNKLNENTKS IYLIPFLVGSALSLLSAIVTRMY+GRNILRSRPE
Sbjct:    1 MFQKDFWVLWLSYTFNSCVAILVLSLHKVFGETLSIIDINLTIASAIGAGFNGIGRIAWGFLIDKFSYKTIMTILTLSMSLMIGTFYLCSLIPNVHQKFIMFLIWVIILHFLLSGNFSIYPVICSRTFGQENSVVNYGVLFTAYALAGLITSSCTTVIFKMTKNHNKLNENTKSYIYLIPFLVGSALSLLSAIVTRMYQGRNILRSRPE 209
[Close]
BLAST of Oxalate:formate antiporter vs. Planmine SMEST
Match: SMESG000061391.1 (SMESG000061391.1)

HSP 1 Score: 221.476 bits (563), Expect = 9.092e-67
Identity = 113/225 (50.22%), Postives = 156/225 (69.33%), Query Frame = 3 
Query:  510 RDSAEIQMKPIKLMFQKDFWVLWLSYTFNSCVAILVLSLHKVFGETLSIIDINLTIASAIGAGFNGIGRIAWGFLIDKFSYKTIMTILTLSMSLMIGTFYLCSLI-----PNVHQKFIMFLIWVIILHFLLSGNFSIYPVICSRTFGQENSVVNYGVLFTAYALAGLITSSCTTVIFKMTKNHNKLNENTKSNIYLIPFLVGSALSLLSAIVTRMYRGRNILRSR 1169
            +++ +I +K  +L+F++DFW+LW++Y  NS VAILVLSLHK FG TL I D+ LT  +A GA  NG+GR+ WG L D+  YK +M+ L+L MSLM+GTFYLC L      P   ++F M+ +WVI+LHFLLSGNF+++P IC++TFG E+   NYG+LFTAY+  G++TS+ TT          KL EN  S  +L PFLVG  LS LS+ VT  YRG++++  R
Sbjct:  232 KENYKISLKTHELLFERDFWILWITYMLNSVVAILVLSLHKTFGFTLKITDLYLTYFTASGAFANGVGRVIWGILNDRIPYKVLMSSLSLCMSLMLGTFYLCVLAGSSNKPESGREF-MYCVWVIVLHFLLSGNFTLFPTICAKTFGSEHFAGNYGLLFTAYSFGGIVTSAVTT----------KLLENEHSIAFLTPFLVGCGLSFLSSGVTLFYRGKSLMYYR 445
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BLAST of Oxalate:formate antiporter vs. Planmine SMEST
Match: SMESG000061391.1 (SMESG000061391.1)

HSP 1 Score: 220.32 bits (560), Expect = 5.628e-66
Identity = 113/224 (50.45%), Postives = 155/224 (69.20%), Query Frame = 3 
Query:  513 DSAEIQMKPIKLMFQKDFWVLWLSYTFNSCVAILVLSLHKVFGETLSIIDINLTIASAIGAGFNGIGRIAWGFLIDKFSYKTIMTILTLSMSLMIGTFYLCSLI-----PNVHQKFIMFLIWVIILHFLLSGNFSIYPVICSRTFGQENSVVNYGVLFTAYALAGLITSSCTTVIFKMTKNHNKLNENTKSNIYLIPFLVGSALSLLSAIVTRMYRGRNILRSR 1169
            ++ +I +K  +L+F++DFW+LW++Y  NS VAILVLSLHK FG TL I D+ LT  +A GA  NG+GR+ WG L D+  YK +M+ L+L MSLM+GTFYLC L      P   ++F M+ +WVI+LHFLLSGNF+++P IC++TFG E+   NYG+LFTAY+  G++TS+ TT          KL EN  S  +L PFLVG  LS LS+ VT  YRG++++  R
Sbjct:  237 ENYKISLKTHELLFERDFWILWITYMLNSVVAILVLSLHKTFGFTLKITDLYLTYFTASGAFANGVGRVIWGILNDRIPYKVLMSSLSLCMSLMLGTFYLCVLAGSSNKPESGREF-MYCVWVIVLHFLLSGNFTLFPTICAKTFGSEHFAGNYGLLFTAYSFGGIVTSAVTT----------KLLENEHSIAFLTPFLVGCGLSFLSSGVTLFYRGKSLMYYR 449
The following BLAST results are available for this feature:
BLAST of Oxalate:formate antiporter vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Oxalate:formate antiporter vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 4
Match NameE-valueIdentityDescription
F10G7.54.293e-1129.41pep chromosome:WBcel235:II:4693414:4696408:1 gene:... [more]
F10G7.54.293e-1129.41pep chromosome:WBcel235:II:4693542:4696408:1 gene:... [more]
F10G7.54.803e-1129.41pep chromosome:WBcel235:II:4693414:4696408:1 gene:... [more]
Y59A8B.211.496e-822.42pep chromosome:WBcel235:V:17986260:17994546:1 gene... [more]
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BLAST of Oxalate:formate antiporter vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Oxalate:formate antiporter vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Oxalate:formate antiporter vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Oxalate:formate antiporter vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Oxalate:formate antiporter vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 2
Match NameE-valueIdentityDescription
sp|Q51330|OXLT_OXAFO2.501e-724.77Oxalate:formate antiporter OS=Oxalobacter formigen... [more]
sp|A0LNN5|SFMCT_SYNFM2.862e-627.36L-lactate transporter OS=Syntrophobacter fumaroxid... [more]
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BLAST of Oxalate:formate antiporter vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0H3YF104.256e-3842.60Slc16a-10 OS=Schmidtea mediterranea OX=79327 GN=sl... [more]
A0A0H3YF062.037e-2634.83Slc16a-5 (Fragment) OS=Schmidtea mediterranea OX=7... [more]
A0A1I8HL502.458e-2432.23MFS domain-containing protein OS=Macrostomum ligna... [more]
A0A267H5U22.557e-2432.23MFS domain-containing protein (Fragment) OS=Macros... [more]
A0A267DK457.340e-2432.23MFS domain-containing protein (Fragment) OS=Macros... [more]
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BLAST of Oxalate:formate antiporter vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Oxalate:formate antiporter vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Oxalate:formate antiporter vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Oxalate:formate antiporter vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Oxalate:formate antiporter vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Oxalate:formate antiporter vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000061390.14.150e-15399.10SMESG000061390.1[more]
SMESG000061390.11.647e-15199.07SMESG000061390.1[more]
SMESG000061390.16.460e-14799.04SMESG000061390.1[more]
SMESG000061391.19.092e-6750.22SMESG000061391.1[more]
SMESG000061391.15.628e-6650.45SMESG000061391.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30020433 ID=SMED30020433|Name=Oxalate:formate antiporter|organism=Schmidtea mediterranea sexual|type=transcript|length=1400bp
TGATAGAGAAATAAAATAATGCATACAAACACAACATATAACATAAAACT
TATGCAAACTTGCAACGTATAATCAATCAGCGTTTAGACAAGCGGTTCCC
AACCTTTCTGGTGCTGTGACCCCTTGATAACATTTGCCAAGTTGTAGCGA
CCCCAAAAATAAAATTAATCGGGTTGCTCCTCAGTTACTGCATTTTTGTA
GCTAACTATTATGTTTTTGACTTCTAAATATCTGATTTTATTTATATTTT
ATAATTTATACTATAAAGTCAGAGTAGGAGTATCGCTTAAATTTAAATCG
CTTAAAATGATACTAAGAAACCACTGACTAGCGTAGGTAAATTATTTTCA
AAAGGTCTCAGGTGACCCGTGGCAATTGTCACGCTACCCCCGCTGGTTTA
GAGGAAAANNNNNNNNNNTNTNNTNNANNTNANNNNNNNNNNAANTNNTN
ANCTNANANTNTNCTNCTNANNNNAATNNTNACNAATNTNNATNANTGNC
NNNNTTNTTAGAGATTCCGCTGAAATCCAAATGAAACCTATTAAGTTAAT
GTTCCAAAAAGATTTTTGGGTGCTGTGGTTATCTTACACTTTTAACTCGT
GCGTAGCGATTCTTGTTTTATCTCTTCATAAAGTTTTTGGAGAGACTTTA
AGCATTATAGACATAAATTTAACAATAGCTTCAGCAATCGGAGCTGGATT
CAATGGAATAGGAAGAATTGCTTGGGGATTCCTTATTGATAAATTTTCAT
ATAAGACCATTATGACAATTTTAACGCTATCTATGTCGTTGATGATTGGA
ACATTTTACCTATGTAGCTTGATACCCAATGTCCATCAAAAATTCATAAT
GTTTTTAATTTGGGTCATTATTTTACATTTCTTGTTGTCGGGAAACTTTT
CCATCTATCCTGTAATTTGTAGTCGAACCTTTGGTCAAGAAAATTCAGTC
GTCAATTATGGAGTCCTCTTCACAGCTTATGCTCTAGCAGGATTAATTAC
AAGCTCATGTACAACTGTCATTTTTAAAATGACTAAAAATCACAATAAAT
TGAACGAAAATACAAAAAGTAATATCTATTTAATACCATTTCTAGTCGGA
AGTGCCCTTTCATTGCTATCGGCTATTGTAACTCGAATGTACCGAGGAAG
GAATATATTAAGAAGTCGACCGGAATAAAGGATATCAAAACTTTGAAAAC
TTTTATGTATATATTAAAAAATTACTAATGAATTTATTTTGATACTATTA
CTTATATTTGAGTTGGTATAAAAAATGATTATGATTAAAAATTCCGATTT
CTAAGCCCACATTCAACAAGGCTCATGCACACGCGCACACTTAAAATCTC
GAGTGGCAGCCAATAGAAAGAACCAATGGCGAAGCGAACCGTTCGGTGTG
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protein sequence of SMED30020433-orf-1

>SMED30020433-orf-1 ID=SMED30020433-orf-1|Name=SMED30020433-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=216bp
MKPIKLMFQKDFWVLWLSYTFNSCVAILVLSLHKVFGETLSIIDINLTIA
SAIGAGFNGIGRIAWGFLIDKFSYKTIMTILTLSMSLMIGTFYLCSLIPN
VHQKFIMFLIWVIILHFLLSGNFSIYPVICSRTFGQENSVVNYGVLFTAY
ALAGLITSSCTTVIFKMTKNHNKLNENTKSNIYLIPFLVGSALSLLSAIV
TRMYRGRNILRSRPE*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0022857transmembrane transporter activity
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR036259MFS_trans_sf
IPR011701MFS
IPR020846MFS_dom
Vocabulary: biological process
TermDefinition
GO:0055085transmembrane transport
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
GO:0016020membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:1.20.1250.20coord: 1..211
e-value: 3.7E-15
score: 57.3
NoneNo IPR availablePANTHERPTHR11360:SF3OXALATE/FORMATE ANTIPORTERcoord: 7..199
NoneNo IPR availablePANTHERPTHR11360MONOCARBOXYLATE TRANSPORTERcoord: 7..199
NoneNo IPR availableCDDcd17353MFS_OFA_likecoord: 1..201
e-value: 1.34736E-25
score: 100.377
NoneNo IPR availableTMHMMTMhelixcoord: 47..69
NoneNo IPR availableTMHMMTMhelixcoord: 108..130
NoneNo IPR availableTMHMMTMhelixcoord: 76..98
NoneNo IPR availableTMHMMTMhelixcoord: 13..32
NoneNo IPR availableTMHMMTMhelixcoord: 180..199
NoneNo IPR availableTMHMMTMhelixcoord: 143..165
IPR036259MFS transporter superfamilySUPERFAMILYSSF103473MFS general substrate transportercoord: 5..207