PiggyBac transposable element-derived protein 4

Overview
NamePiggyBac transposable element-derived protein 4
Smed IDSMED30020369
Length (bp)1452
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of PiggyBac transposable element-derived protein 4 (SMED30020369) t-SNE clustered cells

Violin plots show distribution of expression levels for PiggyBac transposable element-derived protein 4 (SMED30020369) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of PiggyBac transposable element-derived protein 4 (SMED30020369) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for PiggyBac transposable element-derived protein 4 (SMED30020369) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30020369

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30020369SMESG000058633.1 Contig2529newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
head regionSMED30020369SMESG000063657.1 SmedASXL_018433SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
reproductive organSMED30020369SMESG000081574.1 SMESG000081529.1 SMESG000079557.1 SMESG000077556.1 SMESG000073961.1 SMESG000073618.1 SMESG000071198.1 SMESG000068262.1 SMESG000067334.1 SMESG000065669.1 SMESG000060260.1 SMESG000050479.1 SMESG000044971.1 SMESG000043973.1 SMESG000040932.1 SMESG000039792.1 SMESG000039741.1 SMESG000039356.1 SMESG000033763.1 SMESG000029286.1 SMESG000027381.1 SMESG000021522.1 SMESG000019968.1 SMESG000018320.1 SMESG000012464.1 SMESG000007021.1 SMESG000025201.1 Contig12382uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30020369SMESG000081574.1 SMESG000081529.1 SMESG000079557.1 SMESG000077556.1 SMESG000073961.1 SMESG000073618.1 SMESG000071198.1 SMESG000068262.1 SMESG000067334.1 SMESG000065669.1 SMESG000060260.1 SMESG000050479.1 SMESG000044971.1 SMESG000043973.1 SMESG000040932.1 SMESG000039792.1 SMESG000039741.1 SMESG000039356.1 SMESG000033763.1 SMESG000029286.1 SMESG000027381.1 SMESG000021522.1 SMESG000019968.1 SMESG000018320.1 SMESG000012464.1 SMESG000007021.1 SMESG000025201.1 Contig12382newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30020369SMESG000041246.1 SMESG000014208.1 SMESG000010825.1 SMESG000002682.1 Contig11632uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30020369SMESG000041246.1 SMESG000014208.1 SMESG000010825.1 SMESG000002682.1 Contig11632newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Zebrafish
Match: CABZ01072083.2 (dual specificity phosphatase 26 [Source:NCBI gene;Acc:570815])

HSP 1 Score: 63.929 bits (154), Expect = 2.316e-10
Identity = 50/199 (25.13%), Postives = 90/199 (45.23%), Query Frame = 3 
Query:  852 WRASPPNVTRVRDFQILRERQ-----GISVETAQNINTHLVAFKILLPETLINVIVAETNRKARHFYTINSNTARIWYDTNSNEIYALIGILIYAGAHKNRDERLNELY-HLDNRPFYXXXXXXXXXXXXXXXXXXDNYQTRAERLKEDKLASFRDVWNMFLANIKVPYKPSSFLTIDEQLVLTLGRCSLRQYIPSKPG 1430
            W  +PP     R    L E         +   AQ+I +    F++  P+ +  +++  +N + +  Y         W D +  ++ A IGILI  G ++++ E ++ L+  +  RP +RA ++L       R + F        +   DKLA   +VW+ ++  + + Y P   LT+D+ LV   GRC  + Y+  KP 
Sbjct:   90 WSPTPPPSGHKRFPSHLFENMTPGPTSFACSNAQDIKS---TFELFFPDEIKEMLMQISNLEGKRVY------GAAWKDLDWTDLQAFIGILILIGVYRSKHESVDSLWDGVLGRPIFRATMALYTFKSWSRVICFRKRDPEPGQKSGDKLAPILNVWDKWVERLPLMYNPGQNLTVDKCLVPFRGRCPFKIYMQCKPA 279
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BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Xenopus
Match: npffr2 (neuropeptide FF receptor 2 [Source:Xenbase;Acc:XB-GENE-994428])

HSP 1 Score: 56.6102 bits (135), Expect = 4.844e-8
Identity = 27/62 (43.55%), Postives = 35/62 (56.45%), Query Frame = 3 
Query: 1242 DNYQTRAERLKEDKLASFRDVWNMFLANIKVPYKPSSFLTIDEQLVLTLGRCSLRQYIPSKP 1427
            D+  TRAER+K DK A+  D+   F  N    + P   +TIDEQL  T  RC   QY+ +KP
Sbjct:   51 DDKDTRAERVKTDKFAAISDIRTRFNKNCAESFTPGEHMTIDEQLFPTKVRCPFTQYVATKP 112
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. TrEMBL
Match: A0A0V1C353 (PiggyBac transposable element-derived protein 4 (Fragment) OS=Trichinella britovi OX=45882 GN=PGBD4 PE=4 SV=1)

HSP 1 Score: 152.525 bits (384), Expect = 3.353e-38
Identity = 90/204 (44.12%), Postives = 128/204 (62.75%), Query Frame = 3 
Query:  837 KYKRKWRASPPNVTRVRDFQILRERQGISVETAQNINTHLVAFKILLPETLINVIVAETNRKA----RHFYTINSNTARIWYDTNSNEIYALIGILIYAGAHKNRDERLNELYHLDN-RPFYXXXXXXXXXXXXXXXXXXDNYQTRAERLKEDKLASFRDVWNMFLANIKVPYKPSSFLTIDEQLVLTLGRCSLRQYIPSKPGH 1433
            K  R W  S P+ T+ R   I+R+   +   TA  I     A K+L+   ++NVIV  TN+KA    R +   N +  ++W  T+  EIYA IG+L+ +G  ++ DE +++L+ L+N RP +R+ ++  R  +LLRF RFD+  TRA R+K DKLA+FRD+W MF  N K  YKPS+FLT+DEQLV T  R SL+QY+P KPG 
Sbjct:   68 KSGRVWTTSVPSATQTRSHNIVRQMCAV-FTTASGIQCPGDAIKLLITPAMVNVIVQNTNKKAEKVRREWNEKNPDAPKMWKPTDEEEIYAFIGLLLISGVFQSSDESVSDLWSLNNGRPIFRSVMTENRFKELLRFCRFDDNSTRAARIKCDKLAAFRDMWTMFQTNFKKMYKPSAFLTVDEQLVSTRARSSLKQYMPCKPGK 270
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. TrEMBL
Match: A0A0V1C316 (PiggyBac transposable element-derived protein 4 (Fragment) OS=Trichinella britovi OX=45882 GN=PGBD4 PE=4 SV=1)

HSP 1 Score: 152.525 bits (384), Expect = 4.075e-38
Identity = 90/204 (44.12%), Postives = 128/204 (62.75%), Query Frame = 3 
Query:  837 KYKRKWRASPPNVTRVRDFQILRERQGISVETAQNINTHLVAFKILLPETLINVIVAETNRKA----RHFYTINSNTARIWYDTNSNEIYALIGILIYAGAHKNRDERLNELYHLDN-RPFYXXXXXXXXXXXXXXXXXXDNYQTRAERLKEDKLASFRDVWNMFLANIKVPYKPSSFLTIDEQLVLTLGRCSLRQYIPSKPGH 1433
            K  R W  S P+ T+ R   I+R+   +   TA  I     A K+L+   ++NVIV  TN+KA    R +   N +  ++W  T+  EIYA IG+L+ +G  ++ DE +++L+ L+N RP +R+ ++  R  +LLRF RFD+  TRA R+K DKLA+FRD+W MF  N K  YKPS+FLT+DEQLV T  R SL+QY+P KPG 
Sbjct:   73 KSGRVWTTSVPSATQTRSHNIVRQMCAV-FTTASGIQCPGDAIKLLITPAMVNVIVQNTNKKAEKVRREWNEKNPDAPKMWKPTDEEEIYAFIGLLLISGVFQSSDESVSDLWSLNNGRPIFRSVMTENRFKELLRFCRFDDNSTRAARIKCDKLAAFRDMWTMFQTNFKKMYKPSAFLTVDEQLVSTRARSSLKQYMPCKPGK 275
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. TrEMBL
Match: A0A0V0T086 (PiggyBac transposable element-derived protein 4 (Fragment) OS=Trichinella murrelli OX=144512 GN=PGBD4 PE=4 SV=1)

HSP 1 Score: 152.14 bits (383), Expect = 7.450e-38
Identity = 90/204 (44.12%), Postives = 128/204 (62.75%), Query Frame = 3 
Query:  837 KYKRKWRASPPNVTRVRDFQILRERQGISVETAQNINTHLVAFKILLPETLINVIVAETNRKA----RHFYTINSNTARIWYDTNSNEIYALIGILIYAGAHKNRDERLNELYHLDN-RPFYXXXXXXXXXXXXXXXXXXDNYQTRAERLKEDKLASFRDVWNMFLANIKVPYKPSSFLTIDEQLVLTLGRCSLRQYIPSKPGH 1433
            K  R W  S P+ T+ R   I+R+   +   TA  I     A K+L+   ++NVIV  TN+KA    R +   N +  ++W  T+  EIYA IG+L+ +G  ++ DE +++L+ L+N RP +R+ ++  R  +LLRF RFD+  TRA R+K DKLA+FRD+W MF  N K  YKPS+FLT+DEQLV T  R SL+QY+P KPG 
Sbjct:   26 KSGRVWTTSVPSATQTRSHNIVRQMCAV-FTTASGIQCPGDAIKLLITPAMVNVIVQNTNKKAEKVRREWNEKNPDAPKMWKPTDEEEIYAFIGLLLISGVFQSSDESVSDLWSLNNGRPIFRSVMTENRFKELLRFCRFDDNSTRAARIKCDKLAAFRDMWTMFQTNFKKMYKPSAFLTVDEQLVSTRARSSLKQYMPCKPGK 228
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. TrEMBL
Match: A0A0V1PN29 (PiggyBac transposable element-derived protein 4 OS=Trichinella sp. T8 OX=92180 GN=PGBD4 PE=4 SV=1)

HSP 1 Score: 152.91 bits (385), Expect = 3.312e-37
Identity = 90/204 (44.12%), Postives = 129/204 (63.24%), Query Frame = 3 
Query:  837 KYKRKWRASPPNVTRVRDFQILRERQGISVETAQNINTHLVAFKILLPETLINVIVAETNRKA----RHFYTINSNTARIWYDTNSNEIYALIGILIYAGAHKNRDERLNELYHLDN-RPFYXXXXXXXXXXXXXXXXXXDNYQTRAERLKEDKLASFRDVWNMFLANIKVPYKPSSFLTIDEQLVLTLGRCSLRQYIPSKPGH 1433
            K  R W  S P+ T+ R   I+R+   +   TA  I     A K+L+   ++NVIV  TN+KA    R +   N +  ++W  T+  EIYA IG+L+ +G  ++ DE +++L+ L+N RP +R+ ++  R  +LLRF RFD+  TRA R+K DKLA+FRD+W MF  N+K  YKPS+FLT+DEQLV T  R SL+QY+P KPG 
Sbjct:   68 KSGRVWTTSVPSATQTRSHNIVRQMCAV-FTTASGIQCPGDAIKLLITPAMVNVIVQNTNKKAEKVRREWNEKNPDAPKMWKPTDEEEIYAFIGLLLISGVFQSSDESVSDLWSLNNGRPIFRSVMTENRFKELLRFCRFDDNSTRAARIKCDKLAAFRDMWTMFQTNLKKMYKPSAFLTVDEQLVSTRARSSLKQYMPCKPGK 270
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. TrEMBL
Match: A0A0V0RF73 (PiggyBac transposable element-derived protein 4 OS=Trichinella nelsoni OX=6336 GN=PGBD4 PE=4 SV=1)

HSP 1 Score: 152.525 bits (384), Expect = 2.066e-36
Identity = 90/203 (44.33%), Postives = 129/203 (63.55%), Query Frame = 3 
Query:  837 KYKRKWRASPPNVTRVRDFQILRERQGISVETAQNINTHLVAFKILLPETLINVIVAETNRKA----RHFYTINSNTARIWYDTNSNEIYALIGILIYAGAHKNRDERLNELYHLDN-RPFYXXXXXXXXXXXXXXXXXXDNYQTRAERLKEDKLASFRDVWNMFLANIKVPYKPSSFLTIDEQLVLTLGRCSLRQYIPSKPG 1430
            K  R W  S P+ T+ R   I+R+   +   TA  I     A K+L+   ++NVIV  TN+KA    R +   N +  ++W  T+  EIYA IG+L+ +G  ++ DE +++L+ L+N RP +R+ ++  R  +LLRF RFD+  TRA R+K DKLA+FRD+W MF  N+K  YKPS+FLT+DEQLV T  R SL+QY+P KPG
Sbjct:   73 KSGRVWTTSVPSATQTRSHNIVRQMCAV-FTTASGIQCPGDAIKLLITPAMVNVIVQNTNKKAEKVRREWNEKNPDAPKMWKPTDEEEIYAFIGLLLISGVFQSSDESVSDLWSLNNGRPIFRSVMTENRFKELLRFCRFDDNSTRAARIKCDKLAAFRDMWTMFQTNLKKMYKPSAFLTVDEQLVSTRARSSLKQYMPCKPG 274
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Cavefish
Match: nsd3 (nuclear receptor binding SET domain protein 3 [Source:ZFIN;Acc:ZDB-GENE-050324-2])

HSP 1 Score: 85.1149 bits (209), Expect = 3.141e-17
Identity = 54/157 (34.39%), Postives = 86/157 (54.78%), Query Frame = 3 
Query:  960 AFKILLPETLINVIVAETNRKARHFYTINSNTARIWYDTNSNEIYALIGILIYAGAHKNRDERLNELYHLDN-RPFYXXXXXXXXXXXXXXXXXXDNYQTRAERLKEDKLASFRDVWNMFLANIKVPYKPSSFLTIDEQLVLTLGRCSLRQYIPSKP 1427
            +F +     +I +I+  TN   R        +   W D +  EI A +G+LI AG ++++ E    L+   N RP +RA +S +R   +   LRFD+  TR  R KEDKL++F ++W+ ++  + + + P   + +DEQLV   GRC   QY+PSKP
Sbjct:  328 SFDLFFTPDMIQLILQMTNLHGRR-------SVSGWSDVDETEIRAYMGLLILAGVYRSKGESTRSLWDDQNGRPVFRATMSHKRFQLISASLRFDDRLTRPCRHKEDKLSAFCNMWDKWVQRLPLLFNPGENICVDEQLVPFRGRCKFGQYMPSKP 477
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Medaka
Match: ENSORLT00000037833.1 (piggyBac transposable element-derived protein 4-like [Source:NCBI gene;Acc:101160710])

HSP 1 Score: 94.7449 bits (234), Expect = 2.595e-20
Identity = 66/209 (31.58%), Postives = 111/209 (53.11%), Query Frame = 3 
Query:  810 GIWYYAQNKKYKRKWRASP-PNVTRVRDFQILRERQGISVETAQNINTHLVAFKILLPETLINVIVAETNRKARHFYTINSNTARIWYDTNSNEIYALIGILIYAGAHKNRDERLNELYHLDN-RPFYXXXXXXXXXXXXXXXXXXDNYQTRAERLKEDKLASFRDVWNMFLANIKVPYKPSSFLTIDEQLVLTLGRCSLRQYIPSKPG 1430
            G W    +K    KW  SP  +  R+    I++   G +      ++    AF++ + + +  +I+  TN + R  +         W   +  ++ A IGILI AG ++++ E  + L++ +N RP +RA +SL+    + R +RFDN  TR  R + DKLA+ RDVW+ ++  + + Y P   +T+DE+LV   GRC  RQY+P+KP 
Sbjct:   76 GTW---ASKDGNIKWSTSPHQSRGRLSSSNIIKMTPGPTRFAVTRVDDIQSAFQLFISQPIERIILDMTNLEGRRVF------QEKWKSLDQTDLNAYIGILILAGVYRSKGEATSSLWNEENGRPIFRATMSLETFHMISRVIRFDNRDTRVGRRESDKLAAIRDVWDKWVEILPLLYNPGPHVTVDERLVPFRGRCPFRQYMPNKPA 275
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Medaka
Match: ENSORLT00000029842.1 (piggyBac transposable element-derived protein 4 [Source:NCBI gene;Acc:101157644])

HSP 1 Score: 81.6481 bits (200), Expect = 5.935e-16
Identity = 62/203 (30.54%), Postives = 102/203 (50.25%), Query Frame = 3 
Query:  822 YAQNKKYKRKWRASPPNVTRVRDFQILRERQGISVETAQNINTHLVAFKILLPETLINVIVAETNRKARHFYTINSNTARIWYDTNSNEIYALIGILIYAGAHKNRDERLNELYHLD-NRPFYXXXXXXXXXXXXXXXXXXDNYQTRAERLKEDKLASFRDVWNMFLANIKVPYKPSSFLTIDEQLVLTLGRCSLRQYIPSKP 1427
            ++ N++ +   RASPP+           +  G + E  +     L +F +     +  +I+  TNR +       ++  +     +  E+ A +G+LI AG +++R E L  L+  +  RP + A +SL+   Q    LRFD+  TRA R   D+LA+ R+VW  +   +   Y     +T+DE++V   GRCS RQYIPSKP
Sbjct:  338 WSANERDRSSVRASPPH-----------QASGPTTEAREGAQDILSSFLLFFTPNVERLILIATNRHS----ADKAHALKTPKPMDEIELRAYVGLLILAGVYRSRGEALVSLWEPECGRPIFGATMSLRSFYQRSATLRFDDRSTRASRSASDRLAAVREVWEEWCDRLPRLYDIGPEVTVDERMVPFKGRCSFRQYIPSKP 525
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Medaka
Match: ENSORLT00000027888.1 (pep primary_assembly:ASM223467v1:17:9217998:9219211:-1 gene:ENSORLG00000025878.1 transcript:ENSORLT00000027888.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 62.3882 bits (150), Expect = 3.455e-10
Identity = 27/63 (42.86%), Postives = 41/63 (65.08%), Query Frame = 3 
Query: 1242 DNYQTRAERLKEDKLASFRDVWNMFLANIKVPYKPSSFLTIDEQLVLTLGRCSLRQYIPSKPG 1430
            DN  TR  R + DKLA+ RD+W+ ++  + + Y P   +T+DE+LV   GRC  +QY+P+KP 
Sbjct:   16 DNRDTRVGRRESDKLAAIRDLWDKWVEILPLLYNPGPHVTVDERLVPFRGRCPFQQYMPNKPA 78
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. Planmine SMEST
Match: SMESG000032949.1 (SMESG000032949.1)

HSP 1 Score: 73.559 bits (179), Expect = 7.696e-15
Identity = 35/42 (83.33%), Postives = 37/42 (88.10%), Query Frame = 3 
Query:    3 NKLFQEF*NKKYIKIYSTFNQPDERTQGPSHNPIIERFNRTL 128
            NKLFQE  ++  IKIYSTFNQPDERTQG SHNPIIERFNRTL
Sbjct:  144 NKLFQELLDENEIKIYSTFNQPDERTQGSSHNPIIERFNRTL 185
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. Planmine SMEST
Match: SMESG000078863.1 (SMESG000078863.1)

HSP 1 Score: 68.9366 bits (167), Expect = 1.697e-12
Identity = 33/42 (78.57%), Postives = 37/42 (88.10%), Query Frame = 3 
Query:    3 NKLFQEF*NKKYIKIYSTFNQPDERTQGPSHNPIIERFNRTL 128
            NKLFQEF ++  IKIYSTFNQPDERTQGP HN II+RFN+TL
Sbjct:   97 NKLFQEFLDEIDIKIYSTFNQPDERTQGPLHNSIIKRFNKTL 138
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. Planmine SMEST
Match: SMESG000081807.1 (SMESG000081807.1)

HSP 1 Score: 69.3218 bits (168), Expect = 2.058e-12
Identity = 45/115 (39.13%), Postives = 65/115 (56.52%), Query Frame = 3 
Query: 1086 EIYALIGILIYAGAHKNRDERLNEL-YHLDNRPFYXXXXXXXXXXXXXXXXXXDNYQTRAERLKEDKLASFRDVWNMFLANIKVPYKPSSFLTIDEQLVLTLGRCSLRQYIPSKP 1427
            E+ A +GI+I  G   ++ E LN+L + +  RP  R  + L R    L   RFD+  TR +R K DK A+ R++++ F+ N +  Y PS +L +DE  V   G C  R YIPSKP
Sbjct:   36 EMKAAVGIMILVGVMCSKKESLNQLWFEVYGRPLLRTTIPLNRFRIFLSSARFDDKTTRNDRQKTDKFAAIRELFDHFVDNRQELYTPSPYLCVDETQVEFRGGCPFRVYIPSKP 150
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. Planmine SMEST
Match: SMESG000064207.1 (SMESG000064207.1)

HSP 1 Score: 67.781 bits (164), Expect = 2.995e-12
Identity = 32/42 (76.19%), Postives = 36/42 (85.71%), Query Frame = 3 
Query:    3 NKLFQEF*NKKYIKIYSTFNQPDERTQGPSHNPIIERFNRTL 128
            NKLF +F  +  IK+YSTFNQPDERT+G SHNPIIERFNRTL
Sbjct:  224 NKLFSQFLKENDIKMYSTFNQPDERTRGSSHNPIIERFNRTL 265
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. Planmine SMEST
Match: SMESG000027949.1 (SMESG000027949.1)

HSP 1 Score: 64.3142 bits (155), Expect = 3.674e-11
Identity = 30/37 (81.08%), Postives = 33/37 (89.19%), Query Frame = 3 
Query:   15 QEF*NKKYIKIYSTFNQPDERTQGPSHNPIIERFNRT 125
            +EF ++  IKIYSTFNQPDERTQ PSHNPIIERFNRT
Sbjct:  179 KEFLDENDIKIYSTFNQPDERTQSPSHNPIIERFNRT 215
The following BLAST results are available for this feature:
BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
CABZ01072083.22.316e-1025.13dual specificity phosphatase 26 [Source:NCBI gene;... [more]
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BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
npffr24.844e-843.55neuropeptide FF receptor 2 [Source:Xenbase;Acc:XB-... [more]
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BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of PiggyBac transposable element-derived protein 4 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of PiggyBac transposable element-derived protein 4 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0V1C3533.353e-3844.12PiggyBac transposable element-derived protein 4 (F... [more]
A0A0V1C3164.075e-3844.12PiggyBac transposable element-derived protein 4 (F... [more]
A0A0V0T0867.450e-3844.12PiggyBac transposable element-derived protein 4 (F... [more]
A0A0V1PN293.312e-3744.12PiggyBac transposable element-derived protein 4 OS... [more]
A0A0V0RF732.066e-3644.33PiggyBac transposable element-derived protein 4 OS... [more]
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BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
nsd33.141e-1734.39nuclear receptor binding SET domain protein 3 [Sou... [more]
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BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 3
Match NameE-valueIdentityDescription
ENSORLT00000037833.12.595e-2031.58piggyBac transposable element-derived protein 4-li... [more]
ENSORLT00000029842.15.935e-1630.54piggyBac transposable element-derived protein 4 [S... [more]
ENSORLT00000027888.13.455e-1042.86pep primary_assembly:ASM223467v1:17:9217998:921921... [more]
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BLAST of PiggyBac transposable element-derived protein 4 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000032949.17.696e-1583.33SMESG000032949.1[more]
SMESG000078863.11.697e-1278.57SMESG000078863.1[more]
SMESG000081807.12.058e-1239.13SMESG000081807.1[more]
SMESG000064207.12.995e-1276.19SMESG000064207.1[more]
SMESG000027949.13.674e-1181.08SMESG000027949.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30020369 ID=SMED30020369|Name=PiggyBac transposable element-derived protein 4|organism=Schmidtea mediterranea sexual|type=transcript|length=1452bp
ACAATAAGTTATTCCAGGAGTTTTAAAATAAAAAATATATAAAAATCTAT
TCAACATTCAATCAACCAGATGAAAGAACACAAGGTCCATCACATAATCC
TATTATCGAACGATTCAACAGAACACTTAACCTTCAGTCTGTGCATTGGG
GTCAAATGGACCCCAGTCTTTGTAATAAGTGATATAATAAGTTTTTACAC
CAACTAACTATGTCAAGCAACTAGATTTGCCTCAACTAAGGTTTTCCACA
CACATTACAAAAGAAATAATCAAAAATACATTATATATGACGTAGCTACG
ATAGTTTTAAGCAATTTAATTATATTTTGGTGCATTGGGGTTAATCTGAC
CCCAATTTTTTTTCCGCCTAACAATCTATAGAAGTTGTCTCTTTTTCAAA
TGTAAAAAGTGAAATTCAGTGAATATTATTTTTGAATAGAAGTGTTGTTA
TTATAAATTAATTTGTTTGTGATAATTATATATTCATATAAAAAACTATT
TGGTGACTATTTTTATAAGGTGAGAATATTTATATATTTTTGTGGTTGTT
GTGTTTAGTCTAATTTTCTAGGCATATAATAATTTGGATGGCCCGAAAAA
GACAAACTAGCCAGGATATTCGAAATCTATTCGAAAGCATTATCGATTCT
GATGATGAAGAGCTAGCAAATGATAACTTTGATAGTGACTTTTCTGATTT
CGATATAGCAACAGATGAAGCTGCTGTTGAAATGCAAAATATAGAAACTG
ATGAAGAACAAGAAGTACAAAATATAGAAGCTGCTGAAGAAGCTGAAGTG
GAAGAAGAAGGAATTTGGTATTACGCGCAAAATAAAAAGTACAAAAGAAA
ATGGCGGGCAAGTCCACCAAATGTAACTAGAGTTCGCGACTTTCAAATCT
TGAGAGAAAGACAAGGAATTTCTGTTGAAACAGCTCAAAATATAAATACT
CATCTGGTCGCGTTTAAAATATTACTTCCCGAGACATTAATAAATGTAAT
AGTGGCCGAAACAAATAGAAAAGCTCGGCATTTCTACACTATAAATAGTA
ACACTGCACGTATTTGGTATGATACAAATTCAAACGAAATTTATGCACTG
ATTGGTATACTTATATACGCTGGGGCTCATAAGAACCGGGATGAAAGATT
GAATGAATTATACCATCTGGACAACCGTCCTTTTTATCGGGCTGCACTGT
CATTACAAAGAGTGTAGCAGCTTTTACGATTCTTGCGCTTCGACAATTAT
CAAACTAGAGCAGAACGTTTGAAGGAAGATAAGCTAGCATCTTTCCGTGA
TGTATGGAATATGTTTTTGGCAAATATCAAAGTACCTTATAAACCATCAT
CATTCTTAACTATAGATGAACAACTGGTATTAACTCTTGGCCGATGTTCA
CTCAGACAATATATACCATCAAAGCCAGGACATAAAAATTGATCAGACCC
GA
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protein sequence of SMED30020369-orf-1

>SMED30020369-orf-1 ID=SMED30020369-orf-1|Name=SMED30020369-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=210bp
MARKRQTSQDIRNLFESIIDSDDEELANDNFDSDFSDFDIATDEAAVEMQ
NIETDEEQEVQNIEAAEEAEVEEEGIWYYAQNKKYKRKWRASPPNVTRVR
DFQILRERQGISVETAQNINTHLVAFKILLPETLINVIVAETNRKARHFY
TINSNTARIWYDTNSNEIYALIGILIYAGAHKNRDERLNELYHLDNRPFY
RAALSLQRV*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000070intestinal phagocyte
PLANA:0000418head
PLANA:0002089reproductive organ
Vocabulary: INTERPRO
TermDefinition
IPR029526PGBD
IPR019774Aromatic-AA_hydroxylase_C
Vocabulary: molecular function
TermDefinition
GO:0016714oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced pteridine as one donor, and incorporation of one atom of oxygen
Vocabulary: biological process
TermDefinition
GO:0055114oxidation-reduction process