Serine/threonine-protein kinase greatwall
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30020331 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 2
Homology
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Human
Match: MASTL (microtubule associated serine/threonine kinase like [Source:HGNC Symbol;Acc:HGNC:19042]) HSP 1 Score: 219.935 bits (559), Expect = 2.456e-62 Identity = 110/193 (56.99%), Postives = 144/193 (74.61%), Query Frame = 2
Query: 77 PLSINNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGLSKVTLTNTNLQINEILYTPSKSKSDINTEYLRTPNQLYSLM 655
P SI F+I+K I++GA+GKVYLG K K YA+K+V K +M NKN+ HQ+ AER+ALA+S P+IVHLYYSLQ+ NVYLVMEYL GGD+K+L+ + G + A Y E+ +AL+YLH H IIHRDLKPDN+LI+N+GH+KLTDFGLSKVTL N ++ + +IL TPS +K +Y RTP Q+ SL+
Sbjct: 29 PPSIEEFSIVKPISRGAFGKVYLGQKGG---KLYAVKVVKKADMINKNMTHQVQAERDALALSKSPFIVHLYYSLQSANNVYLVMEYLIGGDVKSLLHIYGYFDEEMAVKYISEVALALDYLHRHGIIHRDLKPDNMLISNEGHIKLTDFGLSKVTL-NRDINMMDILTTPSMAKP--RQDYSRTPGQVLSLI 215
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Human
Match: MASTL (microtubule associated serine/threonine kinase like [Source:HGNC Symbol;Acc:HGNC:19042]) HSP 1 Score: 219.55 bits (558), Expect = 5.691e-62 Identity = 110/193 (56.99%), Postives = 144/193 (74.61%), Query Frame = 2
Query: 77 PLSINNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGLSKVTLTNTNLQINEILYTPSKSKSDINTEYLRTPNQLYSLM 655
P SI F+I+K I++GA+GKVYLG K K YA+K+V K +M NKN+ HQ+ AER+ALA+S P+IVHLYYSLQ+ NVYLVMEYL GGD+K+L+ + G + A Y E+ +AL+YLH H IIHRDLKPDN+LI+N+GH+KLTDFGLSKVTL N ++ + +IL TPS +K +Y RTP Q+ SL+
Sbjct: 29 PPSIEEFSIVKPISRGAFGKVYLGQKGG---KLYAVKVVKKADMINKNMTHQVQAERDALALSKSPFIVHLYYSLQSANNVYLVMEYLIGGDVKSLLHIYGYFDEEMAVKYISEVALALDYLHRHGIIHRDLKPDNMLISNEGHIKLTDFGLSKVTL-NRDINMMDILTTPSMAKP--RQDYSRTPGQVLSLI 215 HSP 2 Score: 112.464 bits (280), Expect = 1.778e-25 Identity = 68/182 (37.36%), Postives = 97/182 (53.30%), Query Frame = 2
Query: 980 HPMKASTPLRKKQLNLNDKLPSR---------SSDVLHYLDLTNDGRVLGTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNITKINIDWPDFDLDLS--AVEVINGLLLYDTSKRWGFAQL------NSSDYIDYMRNCSDPPFIPNIAGNDDTGYHDFRN 1474
+PM A TP +K++ + + P++ V + +DGR+LGTPDYL PE+L+ R GP +DWWALGV +FE LTGI PF+ ET +++F NI K +I WP+ + LS A + LL D +KR G +L + D+ + PFIP DT Y + RN
Sbjct: 691 YPM-AITPTQKRRSCMPHQTPNQIKSGTPYRTPKSVRRGVAPVDDGRILGTPDYLAPELLLG---RAHGPAVDWWALGVCLFEFLTGIPPFNDETPQQVFQNILKRDIPWPEGEEKLSDNAQSAVEILLTIDDTKRAGMKELKRHPLFSDVDWENLQHQTM--PFIPQPDDETDTSYFEARN 866
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Human
Match: MASTL (microtubule associated serine/threonine kinase like [Source:HGNC Symbol;Acc:HGNC:19042]) HSP 1 Score: 219.55 bits (558), Expect = 5.919e-62 Identity = 110/193 (56.99%), Postives = 144/193 (74.61%), Query Frame = 2
Query: 77 PLSINNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGLSKVTLTNTNLQINEILYTPSKSKSDINTEYLRTPNQLYSLM 655
P SI F+I+K I++GA+GKVYLG K K YA+K+V K +M NKN+ HQ+ AER+ALA+S P+IVHLYYSLQ+ NVYLVMEYL GGD+K+L+ + G + A Y E+ +AL+YLH H IIHRDLKPDN+LI+N+GH+KLTDFGLSKVTL N ++ + +IL TPS +K +Y RTP Q+ SL+
Sbjct: 29 PPSIEEFSIVKPISRGAFGKVYLGQKGG---KLYAVKVVKKADMINKNMTHQVQAERDALALSKSPFIVHLYYSLQSANNVYLVMEYLIGGDVKSLLHIYGYFDEEMAVKYISEVALALDYLHRHGIIHRDLKPDNMLISNEGHIKLTDFGLSKVTL-NRDINMMDILTTPSMAKP--RQDYSRTPGQVLSLI 215 HSP 2 Score: 112.464 bits (280), Expect = 1.828e-25 Identity = 68/181 (37.57%), Postives = 94/181 (51.93%), Query Frame = 2
Query: 956 HFKTPERKHPMKASTPLRKKQLNLNDKLPSRSSDVLHYLDLTNDGRVLGTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNITKINIDWPDFDLDLS--AVEVINGLLLYDTSKRWGFAQL------NSSDYIDYMRNCSDPPFIPNIAGNDDTGYHDFRN 1474
H +TP + +K+ TP R + V + +DGR+LGTPDYL PE+L+ R GP +DWWALGV +FE LTGI PF+ ET +++F NI K +I WP+ + LS A + LL D +KR G +L + D+ + PFIP DT Y + RN
Sbjct: 707 HQQTPNQ---IKSGTPYRTPK------------SVRRGVAPVDDGRILGTPDYLAPELLLG---RAHGPAVDWWALGVCLFEFLTGIPPFNDETPQQVFQNILKRDIPWPEGEEKLSDNAQSAVEILLTIDDTKRAGMKELKRHPLFSDVDWENLQHQTM--PFIPQPDDETDTSYFEARN 867
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Human
Match: MAST3 (microtubule associated serine/threonine kinase 3 [Source:HGNC Symbol;Acc:HGNC:19036]) HSP 1 Score: 148.288 bits (373), Expect = 4.013e-37 Identity = 73/160 (45.62%), Postives = 108/160 (67.50%), Query Frame = 2
Query: 89 NNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGLSKVTLTN--TNL 562
++F IK I+ GAYG VYL + D + +AIK ++KQ + +N Q+ ER+ L + P++V ++ S +T +++ +VMEY+ GGD TL+ G L + A +Y E ++ALEYLHN+ I+HRDLKPDN+LI + GH+KLTDFGLSK+ L + TNL
Sbjct: 365 SDFETIKLISNGAYGAVYL-VRHRDTRQRFAIKKINKQNLILRNQIQQVFVERDILTFAENPFVVSMFCSFETRRHLCMVMEYVEGGDCATLLKNMGPLPVDMARLYFAETVLALEYLHNYGIVHRDLKPDNLLITSLGHIKLTDFGLSKIGLMSMATNL 523 HSP 2 Score: 96.6709 bits (239), Expect = 2.341e-20 Identity = 49/146 (33.56%), Postives = 78/146 (53.42%), Query Frame = 2
Query: 1085 DGRVLGTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNITKINIDWPDFD--LDLSAVEVINGLLLYDTSKRWGFAQLN---------SSDYIDYMRNCSDPPFIPNIAGNDDTGYHDFRNQIFEY 1489
D +V GTP+Y+ PE++ Y G P+DWWA+GV+++E L G PF G+T EE+FG + I WP+ D L A ++I LL R G + + D+ +R+ ++ F+P + DDT Y D R++ + +
Sbjct: 537 DKQVCGTPEYIAPEVIFRQGY---GKPVDWWAMGVVLYEFLVGCVPFFGDTPEELFGQVVSDEIMWPEGDEALPADAQDLITRLLRQSPLDRLGTGGTHEVKQHPFFLALDWAGLLRHKAE--FVPQLEAEDDTSYFDTRSERYRH 677
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Human
Match: MAST1 (microtubule associated serine/threonine kinase 1 [Source:HGNC Symbol;Acc:HGNC:19034]) HSP 1 Score: 144.821 bits (364), Expect = 5.975e-36 Identity = 74/164 (45.12%), Postives = 109/164 (66.46%), Query Frame = 2
Query: 77 PLSINNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGLSKVTLTN--TNL 562
P N+F IK I+ GAYG VYL + D + +A+K ++KQ + +N Q ER+ L + P++V ++ S +T +++ +VMEY+ GGD TL+ G L ++ A +Y E ++ALEYLHN+ I+HRDLKPDN+LI + GH+KLTDFGLSK+ L + TNL
Sbjct: 368 PPGENDFDTIKLISNGAYGAVYL-VRHRDTRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVGMFCSFETRRHLCMVMEYVEGGDCATLLKNIGALPVEMARMYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHIKLTDFGLSKMGLMSLTTNL 530 HSP 2 Score: 92.8189 bits (229), Expect = 4.231e-19 Identity = 50/146 (34.25%), Postives = 79/146 (54.11%), Query Frame = 2
Query: 1085 DGRVLGTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNITKINIDWPDFD--LDLSAVEVINGLLLYDTSKRWG---------FAQLNSSDYIDYMRNCSDPPFIPNIAGNDDTGYHDFRNQIFEY 1489
D +V GTP+Y+ PE+++ Y G P+DWWA+G+I++E L G PF G+T EE+FG + +I WP+ D L A +I+ LL + R G + D+ +R ++ FIP++ DDT Y D R+ + +
Sbjct: 544 DKQVCGTPEYIAPEVILRQGY---GKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDDILWPEGDEALPTEAQLLISSLLQTNPLVRLGAGGAFEVKQHSFFRDLDWTGLLRQKAE--FIPHLESEDDTSYFDTRSDRYHH 684
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Celegans
Match: wts-1 (Serine/threonine-protein kinase WARTS homolog [Source:UniProtKB/Swiss-Prot;Acc:O45797]) HSP 1 Score: 140.584 bits (353), Expect = 4.164e-35 Identity = 69/151 (45.70%), Postives = 98/151 (64.90%), Query Frame = 2
Query: 80 LSINNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGL 532
+S ++FT+I I GA+GKV L +K+D K YA+K + K ++ K + AER+ LA + P+IV L++S Q + +Y +MEY+ GGD+ TL++ G+ A Y E+ A+EY+HN IHRDLKPDNILI GH+KLTDFGL
Sbjct: 497 MSKSHFTVISHIGVGAFGKVSL-VRKNDTRKVYAMKSLEKADVIMKQQAAHVKAERDILAEADSPWIVRLFFSFQDDACLYFIMEYVPGGDMMTLLIQKGIFEEDLARFYIAELACAIEYVHNVGFIHRDLKPDNILIDQHGHIKLTDFGL 646
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Celegans
Match: kin-4 (KIN-4 protein; Protein KINase [Source:UniProtKB/TrEMBL;Acc:G5EFA4]) HSP 1 Score: 140.584 bits (353), Expect = 4.954e-35 Identity = 66/162 (40.74%), Postives = 105/162 (64.81%), Query Frame = 2
Query: 89 NNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGLSKVTLTNTNLQINE 574
++F I+ ++ GAYG VYL + R ++ A+K ++KQ + +N Q+ AER+ L ++ P++V Y S +T + + ++MEY+ GGD L+ G L ++ +Y E ++A+EYLH++ I+HRDLKPDN+LI GH+KLTDFGLSK+ L N + E
Sbjct: 368 DDFDTIRLVSNGAYGAVYLVRHRETRQRF-ALKKMNKQTLMLRNQVDQVFAERDILTMADNPFVVSFYGSFETRQYLCMLMEYVEGGDCAALLKSAGTLPVELVRLYVAETILAIEYLHSYGIVHRDLKPDNLLITAMGHIKLTDFGLSKIGLMNRTTLVAE 528 HSP 2 Score: 93.2041 bits (230), Expect = 1.266e-19 Identity = 48/150 (32.00%), Postives = 82/150 (54.67%), Query Frame = 2
Query: 1082 NDGRVLGTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNITKINIDWPDFD--LDLSAVEVINGLLLYDTSKRW----GFAQLNSS------DYIDYMRNCSDPPFIPNIAGNDDTGYHDFRNQIFEYSS 1495
D ++ GTP+Y+ PE+++ Y G P+DWWALG+I++E L GI PF GET E +F + ++++P+ D L A ++ LL + ++R G AQL + D+ +R ++ F+P + +DT Y D R + + +
Sbjct: 540 QDKQLCGTPEYIAPEVILRRGY---GKPVDWWALGIILYEFLVGIVPFFGETPEALFSKVISEDVEYPEEDEALPPEAADLCRRLLEKNPAERLGTLNGAAQLMAHEFFILLDFTSLLRQKAE--FVPQLDNEEDTSYFDTRTDRYNHEA 684
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Celegans
Match: sax-1 (Serine/threonine-protein kinase sax-1 [Source:UniProtKB/Swiss-Prot;Acc:Q2L6W9]) HSP 1 Score: 133.265 bits (334), Expect = 1.357e-33 Identity = 63/151 (41.72%), Postives = 99/151 (65.56%), Query Frame = 2
Query: 80 LSINNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGL 532
L++N+F +K I +GA+G+V L +K D YA+K++ K EM K + AER+ L+ + C ++V +YYS Q N+YLVME+L GGD+ TL++ L+ + Y E +A++++H+ IHRD+KPDN+L+ GH+KL+DFGL
Sbjct: 82 LTVNDFESLKVIGRGAFGEVRL-VQKHDTGHIYAMKILRKSEMVEKEQTAHVRAERDILSEADCDWVVKMYYSFQDYSNLYLVMEFLPGGDMMTLLIKKDTLTEEATQFYIAEAALAIQFIHSLGFIHRDIKPDNLLLDARGHVKLSDFGL 231 HSP 2 Score: 55.0694 bits (131), Expect = 7.385e-8 Identity = 25/63 (39.68%), Postives = 36/63 (57.14%), Query Frame = 2
Query: 1094 VLGTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNITKINIDW 1282
+GTPDY+ PE+ + Y DWW+LGVI++EML G PF E +E + + I+W
Sbjct: 280 TVGTPDYIAPEVFQPNGY---TKSCDWWSLGVIMYEMLIGYPPFCSELPQETYRKV----INW 335
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Celegans
Match: sax-1 (Serine/threonine-protein kinase sax-1 [Source:UniProtKB/Swiss-Prot;Acc:Q2L6W9]) HSP 1 Score: 133.265 bits (334), Expect = 1.443e-33 Identity = 63/151 (41.72%), Postives = 99/151 (65.56%), Query Frame = 2
Query: 80 LSINNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGL 532
L++N+F +K I +GA+G+V L +K D YA+K++ K EM K + AER+ L+ + C ++V +YYS Q N+YLVME+L GGD+ TL++ L+ + Y E +A++++H+ IHRD+KPDN+L+ GH+KL+DFGL
Sbjct: 82 LTVNDFESLKVIGRGAFGEVRL-VQKHDTGHIYAMKILRKSEMVEKEQTAHVRAERDILSEADCDWVVKMYYSFQDYSNLYLVMEFLPGGDMMTLLIKKDTLTEEATQFYIAEAALAIQFIHSLGFIHRDIKPDNLLLDARGHVKLSDFGL 231 HSP 2 Score: 54.6842 bits (130), Expect = 9.907e-8 Identity = 25/63 (39.68%), Postives = 36/63 (57.14%), Query Frame = 2
Query: 1094 VLGTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNITKINIDW 1282
+GTPDY+ PE+ + Y DWW+LGVI++EML G PF E +E + + I+W
Sbjct: 280 TVGTPDYIAPEVFQPNGY---TKSCDWWSLGVIMYEMLIGYPPFCSELPQETYRKV----INW 335
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Celegans
Match: kin-4 (KIN-4 protein; Protein KINase [Source:UniProtKB/TrEMBL;Acc:G5EFA4]) HSP 1 Score: 135.576 bits (340), Expect = 2.235e-33 Identity = 66/162 (40.74%), Postives = 105/162 (64.81%), Query Frame = 2
Query: 89 NNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGLSKVTLTNTNLQINE 574
++F I+ ++ GAYG VYL + R ++ A+K ++KQ + +N Q+ AER+ L ++ P++V Y S +T + + ++MEY+ GGD L+ G L ++ +Y E ++A+EYLH++ I+HRDLKPDN+LI GH+KLTDFGLSK+ L N + E
Sbjct: 554 DDFDTIRLVSNGAYGAVYLVRHRETRQRF-ALKKMNKQTLMLRNQVDQVFAERDILTMADNPFVVSFYGSFETRQYLCMLMEYVEGGDCAALLKSAGTLPVELVRLYVAETILAIEYLHSYGIVHRDLKPDNLLITAMGHIKLTDFGLSKIGLMNRTTLVAE 714 HSP 2 Score: 87.8113 bits (216), Expect = 6.151e-18 Identity = 48/149 (32.21%), Postives = 82/149 (55.03%), Query Frame = 2
Query: 1085 DGRVLGTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNITKINIDWPDFD--LDLSAVEVINGLLLYDTSKRWGF----AQLNSS------DYIDYMRNCSDPPFIPNIAGNDDTGYHDFRNQIFEYSS 1495
D ++ GTP+Y+ PE+++ Y G P+DWWALG+I++E L GI PF GET E +F + ++++P+ D L A ++ LL + ++R G AQL + D+ +R ++ F+P + +DT Y D R + + +
Sbjct: 727 DKQLCGTPEYIAPEVILRRGY---GKPVDWWALGIILYEFLVGIVPFFGETPEALFSKVISEDVEYPEEDEALPPEAADLCRRLLEKNPAERLGTLNGAAQLMAHEFFILLDFTSLLRQKAE--FVPQLDNEEDTSYFDTRTDRYNHEA 870
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Fly
Match: gwl (gene:FBgn0260399 transcript:FBtr0331352) HSP 1 Score: 187.963 bits (476), Expect = 2.176e-51 Identity = 95/207 (45.89%), Postives = 138/207 (66.67%), Query Frame = 2
Query: 83 SINNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGLSKVTLTNTNLQINEILYTPSKSKSDINTEYLRTPNQLYSLMDKFELCNTMKTHVFSS 703
+I +F IIK I++GA+GKV+LG K +D + +AIK++ K EM NKN+ Q+ ERNALA+S + V L+YSLQ+ VYLVMEY+ GGDLK+L+ M G A Y E+++AL+YLH H I+HRD+KPDN+L+++ GH+KLTDFGLSK+ + +L+I++++ ++N RTP QL SL + K + F S
Sbjct: 53 TIKDFVIIKPISRGAFGKVFLGYKNNDSKRLFAIKVMRKSEMINKNMVSQVITERNALALSRSQFCVSLFYSLQSLSYVYLVMEYMVGGDLKSLLAMFGYFDEPTARFYVAEMVMALQYLHQHGIVHRDIKPDNMLLSSSGHVKLTDFGLSKIDM-RRDLEISDLI----NCSPNLNA---RTPGQLLSLTSHLSFGSEKKLNDFGS 251 HSP 2 Score: 113.62 bits (283), Expect = 3.072e-26 Identity = 62/165 (37.58%), Postives = 92/165 (55.76%), Query Frame = 2
Query: 1004 LRKKQLNLNDKLPSRSSDVLHYLDLTNDGRVLGTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNITKINIDWPDFD--LDLSAVEVINGLLLYDTSKRWG---FAQLNSSDYIDYMR-NCSDPPFIPNIAGNDDTGYHDFRNQI 1480
L K N+N P R+ + ++ R+LGTPDYL PE+L+ + GP +DWWALGV +E +TGI PF+ ET +++F NI NI+WP+ D L + ++E + LL D ++R Q+ ID+ ++PPF+P DTGY D RN +
Sbjct: 630 LPKTPKNVNINTPFRTPKSVRRGARVSNERILGTPDYLAPELLLKQGH---GPAVDWWALGVCFYEFMTGIPPFNDETPQKVFDNILNKNIEWPEGDEALSVESMEAVELLLTMDPNERPAAKEVQQMRHFACIDWENIGNTEPPFVPTPDNPTDTGYFDARNNL 791
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Fly
Match: gwl (gene:FBgn0260399 transcript:FBtr0083674) HSP 1 Score: 187.578 bits (475), Expect = 3.697e-51 Identity = 95/207 (45.89%), Postives = 138/207 (66.67%), Query Frame = 2
Query: 83 SINNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGLSKVTLTNTNLQINEILYTPSKSKSDINTEYLRTPNQLYSLMDKFELCNTMKTHVFSS 703
+I +F IIK I++GA+GKV+LG K +D + +AIK++ K EM NKN+ Q+ ERNALA+S + V L+YSLQ+ VYLVMEY+ GGDLK+L+ M G A Y E+++AL+YLH H I+HRD+KPDN+L+++ GH+KLTDFGLSK+ + +L+I++++ ++N RTP QL SL + K + F S
Sbjct: 53 TIKDFVIIKPISRGAFGKVFLGYKNNDSKRLFAIKVMRKSEMINKNMVSQVITERNALALSRSQFCVSLFYSLQSLSYVYLVMEYMVGGDLKSLLAMFGYFDEPTARFYVAEMVMALQYLHQHGIVHRDIKPDNMLLSSSGHVKLTDFGLSKIDM-RRDLEISDLI----NCSPNLNA---RTPGQLLSLTSHLSFGSEKKLNDFGS 251 HSP 2 Score: 113.62 bits (283), Expect = 3.611e-26 Identity = 62/165 (37.58%), Postives = 92/165 (55.76%), Query Frame = 2
Query: 1004 LRKKQLNLNDKLPSRSSDVLHYLDLTNDGRVLGTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNITKINIDWPDFD--LDLSAVEVINGLLLYDTSKRWG---FAQLNSSDYIDYMR-NCSDPPFIPNIAGNDDTGYHDFRNQI 1480
L K N+N P R+ + ++ R+LGTPDYL PE+L+ + GP +DWWALGV +E +TGI PF+ ET +++F NI NI+WP+ D L + ++E + LL D ++R Q+ ID+ ++PPF+P DTGY D RN +
Sbjct: 673 LPKTPKNVNINTPFRTPKSVRRGARVSNERILGTPDYLAPELLLKQGH---GPAVDWWALGVCFYEFMTGIPPFNDETPQKVFDNILNKNIEWPEGDEALSVESMEAVELLLTMDPNERPAAKEVQQMRHFACIDWENIGNTEPPFVPTPDNPTDTGYFDARNNL 834
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Fly
Match: dop (gene:FBgn0267390 transcript:FBtr0075562) HSP 1 Score: 142.895 bits (359), Expect = 1.327e-35 Identity = 77/182 (42.31%), Postives = 117/182 (64.29%), Query Frame = 2
Query: 89 NNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGLSKVTLTNTNLQINEILYTPSKSKSDINTEYLRTP 634
N+F I+K I+ GAYG VYL K+ R ++ A+K ++K + +N Q+ AER+ L+ + P++V +Y S +T+K++ LVMEY+ GGD TL+ G L A Y E ++A+EYLH++ I+HRDLKPDN+LI GH+KLTDFGLSK+ L + + E Y S+++ + + TP
Sbjct: 833 NDFDIVKLISNGAYGAVYLVKHKTTRQRF-AMKKINKNNLILRNQVEQVFAERDILSFADNPFVVSMYCSFETKKHLCLVMEYVEGGDCGTLLKNIGPLPADMARFYFAETVLAVEYLHSYGIVHRDLKPDNLLITALGHIKLTDFGLSKMGLMSLATNLYE-GYIDSETRQFSDKQVYGTP 1012 HSP 2 Score: 92.4337 bits (228), Expect = 3.094e-19 Identity = 48/141 (34.04%), Postives = 78/141 (55.32%), Query Frame = 2
Query: 1082 NDGRVLGTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNITKINIDWP---DFDLDLSAVEVINGLLLYDTSKRWGFA----QLNSSDYIDYMRNCS----DPPFIPNIAGNDDTGYHDFR 1471
+D +V GTP+Y+ PE+++ Y G P+DWW++G+I++E L G PF GET EE+F + +I+WP D+ + A ++I+ LL + R G ++ +Y M S F+P ++ +DDT Y D R
Sbjct: 1004 SDKQVYGTPEYIAPEVILRQGY---GKPVDWWSMGIILYEFLIGCVPFFGETTEELFAHTVNDDIEWPDSEDWPVQAEAKDIISQLLQQNPRDRLGTQSGALEMKEHEYFLGMDWNSLLRQKAEFVPQLSHDDDTSYFDTR 1141
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Fly
Match: dop (gene:FBgn0267390 transcript:FBtr0332091) HSP 1 Score: 142.895 bits (359), Expect = 1.327e-35 Identity = 77/182 (42.31%), Postives = 117/182 (64.29%), Query Frame = 2
Query: 89 NNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGLSKVTLTNTNLQINEILYTPSKSKSDINTEYLRTP 634
N+F I+K I+ GAYG VYL K+ R ++ A+K ++K + +N Q+ AER+ L+ + P++V +Y S +T+K++ LVMEY+ GGD TL+ G L A Y E ++A+EYLH++ I+HRDLKPDN+LI GH+KLTDFGLSK+ L + + E Y S+++ + + TP
Sbjct: 833 NDFDIVKLISNGAYGAVYLVKHKTTRQRF-AMKKINKNNLILRNQVEQVFAERDILSFADNPFVVSMYCSFETKKHLCLVMEYVEGGDCGTLLKNIGPLPADMARFYFAETVLAVEYLHSYGIVHRDLKPDNLLITALGHIKLTDFGLSKMGLMSLATNLYE-GYIDSETRQFSDKQVYGTP 1012 HSP 2 Score: 92.4337 bits (228), Expect = 3.094e-19 Identity = 48/141 (34.04%), Postives = 78/141 (55.32%), Query Frame = 2
Query: 1082 NDGRVLGTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNITKINIDWP---DFDLDLSAVEVINGLLLYDTSKRWGFA----QLNSSDYIDYMRNCS----DPPFIPNIAGNDDTGYHDFR 1471
+D +V GTP+Y+ PE+++ Y G P+DWW++G+I++E L G PF GET EE+F + +I+WP D+ + A ++I+ LL + R G ++ +Y M S F+P ++ +DDT Y D R
Sbjct: 1004 SDKQVYGTPEYIAPEVILRQGY---GKPVDWWSMGIILYEFLIGCVPFFGETTEELFAHTVNDDIEWPDSEDWPVQAEAKDIISQLLQQNPRDRLGTQSGALEMKEHEYFLGMDWNSLLRQKAEFVPQLSHDDDTSYFDTR 1141
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Fly
Match: trc (gene:FBgn0003744 transcript:FBtr0074922) HSP 1 Score: 124.79 bits (312), Expect = 1.254e-30 Identity = 61/151 (40.40%), Postives = 93/151 (61.59%), Query Frame = 2
Query: 80 LSINNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGL 532
L + +F +K I +GA+G+V L +K D YA+K++ K +M K + AER+ L + ++V +YYS Q N+YL+ME+L GGD+ TL++ LS + Y E +A++ +H IHRD+KPDN+L+ GHLKL+DFGL
Sbjct: 88 LGVEDFEALKVIGRGAFGEVRL-VQKKDTGHVYAMKVLRKADMLEKEQVAHVRAERDVLVEADHQWVVKMYYSFQDPVNLYLIMEFLPGGDMMTLLMKKDTLSEEGTQFYISETALAIDSIHKLGFIHRDIKPDNLLLDARGHLKLSDFGL 237 HSP 2 Score: 61.6178 bits (148), Expect = 7.380e-10 Identity = 23/56 (41.07%), Postives = 36/56 (64.29%), Query Frame = 2
Query: 1094 VLGTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNI 1261
+GTPDY+ PE+ + + Y GP DWW+LGVI++EML G PF + ++ + +
Sbjct: 289 TVGTPDYIAPEVFLQTGY---GPACDWWSLGVIMYEMLMGYPPFCSDNPQDTYRKV 341
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Zebrafish
Match: mastl (microtubule associated serine/threonine kinase-like [Source:NCBI gene;Acc:445215]) HSP 1 Score: 211.075 bits (536), Expect = 3.081e-59 Identity = 114/225 (50.67%), Postives = 152/225 (67.56%), Query Frame = 2
Query: 83 SINNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGLSKVTLTNTNLQINEILYTPSKSKSDINTEYLRTPNQLYSLMDKFELCNTM---KTHVFSSKRIPQTEWKVKQSHR 748
SI +F ++K I++GA+GKVYL KK + +K YAIK+V K EM +KN+ Q+ AER+ALA+S P+IVHL+YSLQT VYLVMEYL GGD+K+L+ + G + Y E+ +AL+YLH H IIHRDLKPDN+LI+N+GH+KLTDFGLSKV L + L + +IL TPS K +Y RTP Q+ SL+ L + K H + P + KVKQ +R
Sbjct: 19 SIEDFVLVKPISRGAFGKVYLARKKCN-SKLYAIKVVKKAEMVDKNMTEQMRAERDALALSKSPFIVHLFYSLQTATKVYLVMEYLIGGDVKSLLHIYGYFDEDMSLKYISEVALALDYLHRHSIIHRDLKPDNMLISNEGHIKLTDFGLSKVKL-DRELNLMDILTTPSLVKP--TKDYFRTPGQVLSLISSLGLNTPVIEGKRHSSTVLGSPMSCGKVKQRNR 239 HSP 2 Score: 102.064 bits (253), Expect = 2.674e-22 Identity = 64/180 (35.56%), Postives = 91/180 (50.56%), Query Frame = 2
Query: 992 ASTPLRKKQLNLNDKL------------PSRS-SDVLHYLDLTNDGRVLGTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNITKINIDWPDFDLDLS--AVEVINGLLLYDTSKRWGFAQLNSSDYIDYMR----NCSDPPFIPNIAGNDDTGYHDFRN 1474
A TP++KK+ +LN+ L P R+ V + +LGTPDYL PE+L+ + +DWWALGV +FE LTG+ PF+ ET + +F NI +I WPD + +L+ + I LL DT KR G +L + D + + PFIP DT Y + RN
Sbjct: 672 AVTPVQKKRPSLNNSLYQTPQQMVVSHTPYRTPKSVRRGPERVEGAPILGTPDYLAPELLLGKPHDFM---VDWWALGVCLFEFLTGVPPFNDETPQLVFQNILNRDIPWPDGEEELTPNSRNAIEILLTMDTLKRAGLKELKDHPFFDGVDWENLHHQTMPFIPQPDNETDTSYFEARN 848
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Zebrafish
Match: CR356234.1 (microtubule associated serine/threonine kinase 3a [Source:NCBI gene;Acc:100534685]) HSP 1 Score: 145.591 bits (366), Expect = 1.690e-36 Identity = 72/159 (45.28%), Postives = 106/159 (66.67%), Query Frame = 2
Query: 92 NFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGLSKVTLTN--TNL 562
+F IK I+ GAYG VYL + R ++ A+K +++Q + +N Q+ ER+ L + P++V +Y S +T +++ +VMEY+ GGD L+ G L + A +Y E ++ALEYLHN+ I+HRDLKPDN+LI + GH+KLTDFGLSK+ L N TNL
Sbjct: 365 DFETIKLISNGAYGAVYLVRHRETRQRF-AMKKINRQNLILRNQIQQVFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGGDCANLLKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHIKLTDFGLSKIGLMNMTTNL 522 HSP 2 Score: 85.1149 bits (209), Expect = 8.206e-17 Identity = 37/97 (38.14%), Postives = 58/97 (59.79%), Query Frame = 2
Query: 1085 DGRVLGTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNITKINIDWP--DFDLDLSAVEVINGLLLYDTSKRWGFAQ 1369
D ++ GTP+Y+ PE+++ Y G P+DWWA+G+I++E L G PF G+T EE+FG + I+WP D L + ++I LL +R G +
Sbjct: 536 DKQLCGTPEYIAPEVILRQGY---GKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVVSDEIEWPVGDDALPAESQDLITKLLRQSPLERLGTGE 629
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Zebrafish
Match: mast3a (microtubule associated serine/threonine kinase 3a [Source:ZFIN;Acc:ZDB-GENE-040914-35]) HSP 1 Score: 145.591 bits (366), Expect = 2.021e-36 Identity = 72/159 (45.28%), Postives = 106/159 (66.67%), Query Frame = 2
Query: 92 NFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGLSKVTLTN--TNL 562
+F IK I+ GAYG VYL + R ++ A+K +++Q + +N Q+ ER+ L + P++V +Y S +T +++ +VMEY+ GGD L+ G L + A +Y E ++ALEYLHN+ I+HRDLKPDN+LI + GH+KLTDFGLSK+ L N TNL
Sbjct: 376 DFETIKLISNGAYGAVYLVRHRETRQRF-AMKKINRQNLILRNQIQQVFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGGDCANLLKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHIKLTDFGLSKIGLMNMTTNL 533 HSP 2 Score: 85.5001 bits (210), Expect = 6.608e-17 Identity = 37/97 (38.14%), Postives = 58/97 (59.79%), Query Frame = 2
Query: 1085 DGRVLGTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNITKINIDWP--DFDLDLSAVEVINGLLLYDTSKRWGFAQ 1369
D ++ GTP+Y+ PE+++ Y G P+DWWA+G+I++E L G PF G+T EE+FG + I+WP D L + ++I LL +R G +
Sbjct: 547 DKQLCGTPEYIAPEVILRQGY---GKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVVSDEIEWPVGDDALPAESQDLITKLLRQSPLERLGTGE 640
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Zebrafish
Match: mast1a (microtubule associated serine/threonine kinase 1a [Source:ZFIN;Acc:ZDB-GENE-121214-221]) HSP 1 Score: 145.206 bits (365), Expect = 2.424e-36 Identity = 73/164 (44.51%), Postives = 108/164 (65.85%), Query Frame = 2
Query: 77 PLSINNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGLSKVTLTN--TNL 562
P ++F IK I+ GAYG VYL + R ++ A+K ++KQ + +N Q ER+ L + P++V ++ S +T +++ +VMEY+ GGD TL+ G L + A +Y E ++ALEY+HN+ I+HRDLKPDN+LI + GH+KLTDFGLSK+ L N TNL
Sbjct: 343 PPCESDFQTIKLISNGAYGAVYLVRHRETRQRF-AMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMFCSFETRRHLCMVMEYVEGGDCATLLKNIGALPVDMARMYFAETVLALEYIHNYGIVHRDLKPDNLLITSMGHIKLTDFGLSKMGLMNLTTNL 505 HSP 2 Score: 96.2857 bits (238), Expect = 2.607e-20 Identity = 49/146 (33.56%), Postives = 80/146 (54.79%), Query Frame = 2
Query: 1085 DGRVLGTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNITKINIDWPDFD--LDLSAVEVINGLLLYDTSKRWGFAQ---------LNSSDYIDYMRNCSDPPFIPNIAGNDDTGYHDFRNQIFEY 1489
D +V GTP+Y+ PE+++ Y G P+DWWA+G+I++E L G PF G+T EE+FG + I+WP+ D L A +I+ LL + R G + D+ +R ++ FIP++ +DT Y D R++ + +
Sbjct: 519 DKQVCGTPEYIAPEVILRQGY---GKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVITDYIEWPNGDEALPADAQSLISSLLQTNPLVRLGTGGAFEVKQHSFFSGLDWNGLLRQKAE--FIPHLESEEDTSYFDTRSERYRH 659
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Zebrafish
Match: mast1a (microtubule associated serine/threonine kinase 1a [Source:ZFIN;Acc:ZDB-GENE-121214-221]) HSP 1 Score: 145.206 bits (365), Expect = 2.827e-36 Identity = 73/164 (44.51%), Postives = 108/164 (65.85%), Query Frame = 2
Query: 77 PLSINNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGLSKVTLTN--TNL 562
P ++F IK I+ GAYG VYL + R ++ A+K ++KQ + +N Q ER+ L + P++V ++ S +T +++ +VMEY+ GGD TL+ G L + A +Y E ++ALEY+HN+ I+HRDLKPDN+LI + GH+KLTDFGLSK+ L N TNL
Sbjct: 355 PPCESDFQTIKLISNGAYGAVYLVRHRETRQRF-AMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMFCSFETRRHLCMVMEYVEGGDCATLLKNIGALPVDMARMYFAETVLALEYIHNYGIVHRDLKPDNLLITSMGHIKLTDFGLSKMGLMNLTTNL 517 HSP 2 Score: 96.2857 bits (238), Expect = 2.742e-20 Identity = 49/146 (33.56%), Postives = 80/146 (54.79%), Query Frame = 2
Query: 1085 DGRVLGTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNITKINIDWPDFD--LDLSAVEVINGLLLYDTSKRWGFAQ---------LNSSDYIDYMRNCSDPPFIPNIAGNDDTGYHDFRNQIFEY 1489
D +V GTP+Y+ PE+++ Y G P+DWWA+G+I++E L G PF G+T EE+FG + I+WP+ D L A +I+ LL + R G + D+ +R ++ FIP++ +DT Y D R++ + +
Sbjct: 531 DKQVCGTPEYIAPEVILRQGY---GKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVITDYIEWPNGDEALPADAQSLISSLLQTNPLVRLGTGGAFEVKQHSFFSGLDWNGLLRQKAE--FIPHLESEEDTSYFDTRSERYRH 671
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Xenopus
Match: cachd1 (cache domain containing 1 [Source:Xenbase;Acc:XB-GENE-1010398]) HSP 1 Score: 212.616 bits (540), Expect = 1.520e-59 Identity = 105/198 (53.03%), Postives = 145/198 (73.23%), Query Frame = 2
Query: 62 WILNMPLSINNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGLSKVTLTNTNLQINEILYTPSKSKSDINTEYLRTPNQLYSLM 655
+ + P SI F+I+K I++GA+GKVYL +K++ K +A+K+V K +M NKN+ Q+ AER+ALA+S P+IVHLYYSLQ+ N+YL+MEYL GGD+K+L+ + G + A Y E+ +AL+YLH H IIHRDLKPDN+LI+N+GH+KLTDFGLSKVTL L + +IL TPS +K +Y RTP Q+ SL+
Sbjct: 22 FTVPQPPSIEEFSIVKPISRGAFGKVYLARRKNN-NKLFAVKVVKKADMINKNMVQQVQAERDALALSKSPFIVHLYYSLQSANNIYLIMEYLIGGDVKSLLHIYGYFDEEMAVKYISEVALALDYLHRHGIIHRDLKPDNMLISNEGHIKLTDFGLSKVTLKR-ELSMMDILTTPSMAKP--KRDYSRTPGQVLSLI 215 HSP 2 Score: 107.071 bits (266), Expect = 9.975e-24 Identity = 68/172 (39.53%), Postives = 92/172 (53.49%), Query Frame = 2
Query: 983 PMKASTPLRKKQLNLNDKLPSRSSDVLHYLDL-TNDGRVLGTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNITKINIDWPDFD--LDLSAVEVINGLLLYDTSKRWGFAQLNSSDYI-----DYMRNCSDPPFIPNIAGNDDTGYHDFRN 1474
PM A TP +KK L + P R+ + L + R+LGTPDYL PE+L+ + GP +DWWALGV +FE LTGI PF+ ET ++F NI +I WP+ + L ++A I LL D +KR G L + D ++N PFIP DT Y + RN
Sbjct: 713 PM-AVTPAQKKVQGLTET-PYRTPKTVRRGGLQAENERILGTPDYLAPELLLGKSH---GPAVDWWALGVCLFEFLTGIPPFNDETPSQVFQNILNRDIPWPEEEETLSVNAQSAIEILLAIDQTKRAGLKDLKAHHLFHAIEWDDLQNLPM-PFIPQPDDETDTTYFEARN 878
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Xenopus
Match: mast2 (microtubule associated serine/threonine kinase 2 [Source:NCBI gene;Acc:779595]) HSP 1 Score: 142.51 bits (358), Expect = 2.754e-35 Identity = 73/162 (45.06%), Postives = 107/162 (66.05%), Query Frame = 2
Query: 83 SINNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGLSKVTLTN--TNL 562
S F IK I+ GAYG V+L K R ++ A+K ++KQ + +N Q ER+ L + P++V ++ S +T++++ +VMEY+ GGD TL+ G L + A +Y E ++ALEYLHN+ I+HRDLKPDN+LI + GH+KLTDFGLSK+ L + TNL
Sbjct: 456 SEEQFENIKLISNGAYGAVHLVRHKETRQRF-AMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMFCSFETKRHLCMVMEYVEGGDCATLLKSIGALPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHIKLTDFGLSKIGLMSLTTNL 616 HSP 2 Score: 103.605 bits (257), Expect = 1.625e-22 Identity = 52/146 (35.62%), Postives = 80/146 (54.79%), Query Frame = 2
Query: 1085 DGRVLGTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNITKINIDWPDFD--LDLSAVEVINGLLLYDTSKRWGFAQ---------LNSSDYIDYMRNCSDPPFIPNIAGNDDTGYHDFRNQIFEY 1489
D +V GTP+Y+ PE+++ Y G P+DWWA+GVI++E L G PF G+T EE+FG + I WPD D L A ++I+ LL + +R G D+ +R ++ FIP + DDT Y D R++ +++
Sbjct: 630 DKQVCGTPEYIAPEVILRQGY---GKPVDWWAMGVILYEFLVGCVPFFGDTPEELFGQVISDEIAWPDGDDALPADAQDLISRLLRQNPLERLGTGSAIEVKQHRFFKDLDWNGLLRQKAE--FIPQLESEDDTSYFDTRSERYQH 770
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Xenopus
Match: LATS1 (large tumor suppressor kinase 1 [Source:NCBI gene;Acc:100145677]) HSP 1 Score: 128.642 bits (322), Expect = 8.172e-31 Identity = 63/146 (43.15%), Postives = 93/146 (63.70%), Query Frame = 2
Query: 95 FTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGL 532
F IK + GA+G+V L +K+D YA+K + K+++ +N + AER+ LA + ++V LYYS Q + N+Y VM+Y+ GGD+ +L++ G+ A Y E+ A+E +H IHRD+KPDNILI DGH+KLTDFGL
Sbjct: 690 FVKIKTLGIGAFGEVCLA-RKTDTNALYAMKTLRKKDVLLRNQVAHVKAERDILAEADNEWVVRLYYSFQDKDNLYFVMDYIPGGDMMSLLIRMGVFPEDLARFYIAELTCAVESVHKMDFIHRDIKPDNILIDRDGHIKLTDFGL 834 HSP 2 Score: 51.2174 bits (121), Expect = 3.729e-6 Identity = 21/51 (41.18%), Postives = 34/51 (66.67%), Query Frame = 2
Query: 1094 VLGTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEE 1246
++GTP+Y+ PE+L+ + Y DWW++GVI++EML G PF ++ E
Sbjct: 895 LVGTPNYIAPEVLLRTGYTQL---CDWWSVGVILYEMLVGQPPFLAQSPVE 942
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Xenopus
Match: stk38l (serine/threonine kinase 38 like [Source:NCBI gene;Acc:448427]) HSP 1 Score: 124.79 bits (312), Expect = 1.311e-30 Identity = 59/151 (39.07%), Postives = 96/151 (63.58%), Query Frame = 2
Query: 80 LSINNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGL 532
L + +F +K I +GA+G+V L +K D YA+K++ K +M K I AER+ L + ++V ++YS Q ++N+YL+ME+L GGD+ TL++ L+ + Y E ++A++ +H IHRD+KPDN+L+ GH+KL+DFGL
Sbjct: 33 LGLEDFESLKVIGRGAFGEVRL-VQKKDTGHIYAMKILRKADMLEKEQVAHIRAERDILVEADGAWVVKMFYSFQDKRNLYLIMEFLPGGDMMTLLMKKDTLTEEATQFYIAETVLAIDAIHQLGFIHRDIKPDNLLLDAKGHVKLSDFGL 182 HSP 2 Score: 58.151 bits (139), Expect = 1.744e-8 Identity = 24/56 (42.86%), Postives = 35/56 (62.50%), Query Frame = 2
Query: 1094 VLGTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNI 1261
+GTPDY+ PE+ + + Y DWW+LGVI++EML G PF ET +E + +
Sbjct: 231 TVGTPDYIAPEVFMQTGY---NKLCDWWSLGVIMYEMLIGYPPFCSETPQETYRKV 283
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Xenopus
Match: stk38l (serine/threonine kinase 38 like [Source:NCBI gene;Acc:448427]) HSP 1 Score: 124.79 bits (312), Expect = 1.800e-30 Identity = 59/151 (39.07%), Postives = 96/151 (63.58%), Query Frame = 2
Query: 80 LSINNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGL 532
L + +F +K I +GA+G+V L +K D YA+K++ K +M K I AER+ L + ++V ++YS Q ++N+YL+ME+L GGD+ TL++ L+ + Y E ++A++ +H IHRD+KPDN+L+ GH+KL+DFGL
Sbjct: 81 LGLEDFESLKVIGRGAFGEVRL-VQKKDTGHIYAMKILRKADMLEKEQVAHIRAERDILVEADGAWVVKMFYSFQDKRNLYLIMEFLPGGDMMTLLMKKDTLTEEATQFYIAETVLAIDAIHQLGFIHRDIKPDNLLLDAKGHVKLSDFGL 230 HSP 2 Score: 58.5362 bits (140), Expect = 1.332e-8 Identity = 24/56 (42.86%), Postives = 35/56 (62.50%), Query Frame = 2
Query: 1094 VLGTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNI 1261
+GTPDY+ PE+ + + Y DWW+LGVI++EML G PF ET +E + +
Sbjct: 279 TVGTPDYIAPEVFMQTGY---NKLCDWWSLGVIMYEMLIGYPPFCSETPQETYRKV 331
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Mouse
Match: Mastl (microtubule associated serine/threonine kinase-like [Source:MGI Symbol;Acc:MGI:1914371]) HSP 1 Score: 219.55 bits (558), Expect = 2.689e-62 Identity = 109/193 (56.48%), Postives = 144/193 (74.61%), Query Frame = 2
Query: 77 PLSINNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGLSKVTLTNTNLQINEILYTPSKSKSDINTEYLRTPNQLYSLM 655
P SI FTI+K I++GA+GKVYLG K K YA+K+V K +M NKN+ HQ+ AER+ALA+S P++VHLYYSLQ+ N+YL+MEYL GGD+K+L+ + G + A Y E+ +AL+YLH H IIHRDLKPDN+LI+N+GH+KLTDFGLSKVTL N ++ + +IL TPS SK +Y RTP Q+ SL+
Sbjct: 28 PPSIEEFTIVKPISRGAFGKVYLGQKGG---KLYAVKVVKKADMINKNMTHQVQAERDALALSKSPFVVHLYYSLQSASNIYLIMEYLIGGDVKSLLHIYGYFDEEMAIKYISEVALALDYLHRHGIIHRDLKPDNMLISNEGHIKLTDFGLSKVTL-NRDINMMDILTTPSMSKP--KQDYSRTPGQVLSLI 214 HSP 2 Score: 112.464 bits (280), Expect = 1.297e-25 Identity = 59/139 (42.45%), Postives = 82/139 (58.99%), Query Frame = 2
Query: 1082 NDGRVLGTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNITKINIDWPDFDLDLS--AVEVINGLLLYDTSKRWGFAQL------NSSDYIDYMRNCSDPPFIPNIAGNDDTGYHDFRN 1474
+DGR+LGTPDYL PE+L+ + + GP +DWWALGV +FE LTGI PF+ ET +++F NI K +I WP+ + LS A ++ LL D SKR G +L + D+ + PF+P DT Y + RN
Sbjct: 720 DDGRILGTPDYLAPELLLGTAH---GPAVDWWALGVCLFEFLTGIPPFNDETPQQVFQNILKRDIPWPEGEEKLSDNAQSAMDMLLTIDDSKRAGMRELKQHPLFSEVDWENLQHQTM--PFVPQPDDETDTSYFEARN 853
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Mouse
Match: Mast3 (microtubule associated serine/threonine kinase 3 [Source:MGI Symbol;Acc:MGI:2683541]) HSP 1 Score: 147.902 bits (372), Expect = 3.517e-37 Identity = 73/160 (45.62%), Postives = 108/160 (67.50%), Query Frame = 2
Query: 89 NNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGLSKVTLTN--TNL 562
++F IK I+ GAYG VYL + D + +AIK ++KQ + +N Q+ ER+ L + P++V ++ S +T +++ +VMEY+ GGD TL+ G L + A +Y E ++ALEYLHN+ I+HRDLKPDN+LI + GH+KLTDFGLSK+ L + TNL
Sbjct: 387 SDFETIKLISNGAYGAVYL-VRHRDTRQRFAIKKINKQNLILRNQIQQVFVERDILTFAENPFVVGMFCSFETRRHLCMVMEYVEGGDCATLLKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLITSLGHIKLTDFGLSKIGLMSMATNL 545 HSP 2 Score: 99.3673 bits (246), Expect = 2.617e-21 Identity = 51/146 (34.93%), Postives = 79/146 (54.11%), Query Frame = 2
Query: 1085 DGRVLGTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNITKINIDWPDFD--LDLSAVEVINGLLLYDTSKRWGFAQLN---------SSDYIDYMRNCSDPPFIPNIAGNDDTGYHDFRNQIFEY 1489
D +V GTP+Y+ PE++ Y G P+DWWA+GVI++E L G PF G+T EE+FG + I WP+ D L L A ++I LL R G + + D+ +R+ ++ F+P + DDT Y D R++ + +
Sbjct: 559 DKQVCGTPEYIAPEVIFRQGY---GKPVDWWAMGVILYEFLVGCVPFFGDTPEELFGQVVSDEIMWPEGDEALPLDAQDLITRLLRQSPMDRLGTGGTHEVKQHPFFLALDWAGLLRHKAE--FVPQLEAEDDTSYFDTRSERYRH 699
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Mouse
Match: Mast3 (microtubule associated serine/threonine kinase 3 [Source:MGI Symbol;Acc:MGI:2683541]) HSP 1 Score: 147.902 bits (372), Expect = 3.517e-37 Identity = 73/160 (45.62%), Postives = 108/160 (67.50%), Query Frame = 2
Query: 89 NNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGLSKVTLTN--TNL 562
++F IK I+ GAYG VYL + D + +AIK ++KQ + +N Q+ ER+ L + P++V ++ S +T +++ +VMEY+ GGD TL+ G L + A +Y E ++ALEYLHN+ I+HRDLKPDN+LI + GH+KLTDFGLSK+ L + TNL
Sbjct: 387 SDFETIKLISNGAYGAVYL-VRHRDTRQRFAIKKINKQNLILRNQIQQVFVERDILTFAENPFVVGMFCSFETRRHLCMVMEYVEGGDCATLLKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLITSLGHIKLTDFGLSKIGLMSMATNL 545 HSP 2 Score: 99.3673 bits (246), Expect = 2.617e-21 Identity = 51/146 (34.93%), Postives = 79/146 (54.11%), Query Frame = 2
Query: 1085 DGRVLGTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNITKINIDWPDFD--LDLSAVEVINGLLLYDTSKRWGFAQLN---------SSDYIDYMRNCSDPPFIPNIAGNDDTGYHDFRNQIFEY 1489
D +V GTP+Y+ PE++ Y G P+DWWA+GVI++E L G PF G+T EE+FG + I WP+ D L L A ++I LL R G + + D+ +R+ ++ F+P + DDT Y D R++ + +
Sbjct: 559 DKQVCGTPEYIAPEVIFRQGY---GKPVDWWAMGVILYEFLVGCVPFFGDTPEELFGQVVSDEIMWPEGDEALPLDAQDLITRLLRQSPMDRLGTGGTHEVKQHPFFLALDWAGLLRHKAE--FVPQLEAEDDTSYFDTRSERYRH 699
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Mouse
Match: Mast3 (microtubule associated serine/threonine kinase 3 [Source:MGI Symbol;Acc:MGI:2683541]) HSP 1 Score: 147.517 bits (371), Expect = 4.206e-37 Identity = 73/160 (45.62%), Postives = 108/160 (67.50%), Query Frame = 2
Query: 89 NNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGLSKVTLTN--TNL 562
++F IK I+ GAYG VYL + D + +AIK ++KQ + +N Q+ ER+ L + P++V ++ S +T +++ +VMEY+ GGD TL+ G L + A +Y E ++ALEYLHN+ I+HRDLKPDN+LI + GH+KLTDFGLSK+ L + TNL
Sbjct: 371 SDFETIKLISNGAYGAVYL-VRHRDTRQRFAIKKINKQNLILRNQIQQVFVERDILTFAENPFVVGMFCSFETRRHLCMVMEYVEGGDCATLLKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLITSLGHIKLTDFGLSKIGLMSMATNL 529 HSP 2 Score: 99.3673 bits (246), Expect = 2.629e-21 Identity = 51/146 (34.93%), Postives = 79/146 (54.11%), Query Frame = 2
Query: 1085 DGRVLGTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNITKINIDWPDFD--LDLSAVEVINGLLLYDTSKRWGFAQLN---------SSDYIDYMRNCSDPPFIPNIAGNDDTGYHDFRNQIFEY 1489
D +V GTP+Y+ PE++ Y G P+DWWA+GVI++E L G PF G+T EE+FG + I WP+ D L L A ++I LL R G + + D+ +R+ ++ F+P + DDT Y D R++ + +
Sbjct: 543 DKQVCGTPEYIAPEVIFRQGY---GKPVDWWAMGVILYEFLVGCVPFFGDTPEELFGQVVSDEIMWPEGDEALPLDAQDLITRLLRQSPMDRLGTGGTHEVKQHPFFLALDWAGLLRHKAE--FVPQLEAEDDTSYFDTRSERYRH 683
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Mouse
Match: Mast3 (microtubule associated serine/threonine kinase 3 [Source:MGI Symbol;Acc:MGI:2683541]) HSP 1 Score: 147.517 bits (371), Expect = 4.206e-37 Identity = 73/160 (45.62%), Postives = 108/160 (67.50%), Query Frame = 2
Query: 89 NNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGLSKVTLTN--TNL 562
++F IK I+ GAYG VYL + D + +AIK ++KQ + +N Q+ ER+ L + P++V ++ S +T +++ +VMEY+ GGD TL+ G L + A +Y E ++ALEYLHN+ I+HRDLKPDN+LI + GH+KLTDFGLSK+ L + TNL
Sbjct: 371 SDFETIKLISNGAYGAVYL-VRHRDTRQRFAIKKINKQNLILRNQIQQVFVERDILTFAENPFVVGMFCSFETRRHLCMVMEYVEGGDCATLLKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLITSLGHIKLTDFGLSKIGLMSMATNL 529 HSP 2 Score: 99.3673 bits (246), Expect = 2.629e-21 Identity = 51/146 (34.93%), Postives = 79/146 (54.11%), Query Frame = 2
Query: 1085 DGRVLGTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNITKINIDWPDFD--LDLSAVEVINGLLLYDTSKRWGFAQLN---------SSDYIDYMRNCSDPPFIPNIAGNDDTGYHDFRNQIFEY 1489
D +V GTP+Y+ PE++ Y G P+DWWA+GVI++E L G PF G+T EE+FG + I WP+ D L L A ++I LL R G + + D+ +R+ ++ F+P + DDT Y D R++ + +
Sbjct: 543 DKQVCGTPEYIAPEVIFRQGY---GKPVDWWAMGVILYEFLVGCVPFFGDTPEELFGQVVSDEIMWPEGDEALPLDAQDLITRLLRQSPMDRLGTGGTHEVKQHPFFLALDWAGLLRHKAE--FVPQLEAEDDTSYFDTRSERYRH 683
BLAST of Serine/threonine-protein kinase greatwall vs. UniProt/SwissProt
Match: sp|E2RJI4|GWL_CANLF (Serine/threonine-protein kinase greatwall OS=Canis lupus familiaris OX=9615 GN=MASTL PE=3 SV=1) HSP 1 Score: 225.328 bits (573), Expect = 1.866e-63 Identity = 118/227 (51.98%), Postives = 155/227 (68.28%), Query Frame = 2
Query: 77 PLSINNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGLSKVTLTNTNLQIN--EILYTPSKSKSDINTEYLRTPNQLYSLMDKFEL-----------CNTMKTHVFSSKRIPQ 718
P SI FTI+K I++GA+GKVYLG K K YA+K+V K +M NKN+ HQ+ AER+ALA+S P+IVHLYYSLQ+ NVYLVMEYL GGD+K+L+ + G + A Y E+ +AL+YLH H IIHRDLKPDN+LI+N+GH+KLTDFGLSK+TL N ++ IN +IL TPS +K +Y RTP Q+ SL+ N + THVF + ++ Q
Sbjct: 29 PPSIEEFTIVKPISRGAFGKVYLGQKGG---KLYAVKVVKKADMINKNMTHQVQAERDALALSKSPFIVHLYYSLQSANNVYLVMEYLIGGDVKSLLHIYGYFDEEMAVKYISEVALALDYLHRHGIIHRDLKPDNMLISNEGHIKLTDFGLSKITL-NRDIDINMMDILTTPSMAKP--RQDYSRTPGQVLSLISSLRFYTPAAEKDKDSANILSTHVFETSQLSQ 249 HSP 2 Score: 110.153 bits (274), Expect = 4.531e-24 Identity = 61/138 (44.20%), Postives = 80/138 (57.97%), Query Frame = 2
Query: 1082 NDGRVLGTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNITKINIDWPDFDLDLS--AVEVINGLLLYDTSKRWGFAQLNS----SDYIDYMR-NCSDPPFIPNIAGNDDTGYHDFRN 1474
+D R+LGTPDYL PE+L+ R GP +DWWALGV +FE LTGI PF+ ET +++F NI K +I WP+ + LS A + LL D +KR G +L SD +D+ PFIP DT Y + RN
Sbjct: 738 DDARILGTPDYLAPELLLG---RAHGPAVDWWALGVCLFEFLTGIPPFNDETSQQVFQNILKRDIPWPEGEEKLSDNAQNAVEILLTIDNAKRAGIKELKCHPLFSD-VDWENLQHQTMPFIPQPDDETDTSYFEARN 871
BLAST of Serine/threonine-protein kinase greatwall vs. UniProt/SwissProt
Match: sp|D2HXI8|GWL_AILME (Serine/threonine-protein kinase greatwall OS=Ailuropoda melanoleuca OX=9646 GN=MASTL PE=3 SV=1) HSP 1 Score: 222.246 bits (565), Expect = 2.378e-62 Identity = 116/225 (51.56%), Postives = 154/225 (68.44%), Query Frame = 2
Query: 77 PLSINNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGLSKVTLTNTNLQINEILYTPSKSKSDINTEYLRTPNQLYSLMDKF-----------ELCNTMKTHVFSSKRIPQ 718
P SI FTI+K I++GA+GKVYLG K K YA+K+V K +M NKN+ HQ+ AER+ALA+S P+IVHLYYSLQ+ NVYLVMEYL GGD+K+L+ + G + A Y E+ +AL+YLH H IIHRDLKPDN+LI+N+GH+KLTDFGLSKVTL N ++ + +IL TPS +K +Y RTP Q+ SL+ + N + THV + ++ Q
Sbjct: 29 PPSIEEFTIVKPISRGAFGKVYLGQKGG---KLYAVKVVKKADMINKNMTHQVQAERDALALSKSPFIVHLYYSLQSANNVYLVMEYLIGGDVKSLLHIYGYFDEEMAVKYISEVALALDYLHRHGIIHRDLKPDNMLISNEGHIKLTDFGLSKVTL-NRDINMMDILTTPSMAKP--RQDYSRTPGQVLSLISSLGFFTPVAEKNKDSANILSTHVSETSQLSQ 247 HSP 2 Score: 111.309 bits (277), Expect = 2.146e-24 Identity = 62/142 (43.66%), Postives = 82/142 (57.75%), Query Frame = 2
Query: 1082 NDGRVLGTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNITKINIDWPDFDLDLS-----AVEVINGLLLYDTSKRWGFAQL------NSSDYIDYMRNCSDPPFIPNIAGNDDTGYHDFRN 1474
+DGR+LGTPDYL PE+L+ R GP +DWWALGV +FE LTGI PF+ ET +++F NI K +I WP+ + LS AVE+ LL D +KR G +L + D+ + PFIP DT Y + RN
Sbjct: 737 DDGRILGTPDYLAPELLLG---RAHGPAVDWWALGVCLFEFLTGIPPFNDETPQQVFQNILKRDIPWPEGEEKLSDNSQNAVEI---LLTIDNAKRAGMKELKRHHLFSDVDWENLQHQTM--PFIPQPDDETDTSYFEARN 870
BLAST of Serine/threonine-protein kinase greatwall vs. UniProt/SwissProt
Match: sp|Q8C0P0|GWL_MOUSE (Serine/threonine-protein kinase greatwall OS=Mus musculus OX=10090 GN=Mastl PE=2 SV=2) HSP 1 Score: 219.55 bits (558), Expect = 1.881e-61 Identity = 109/193 (56.48%), Postives = 144/193 (74.61%), Query Frame = 2
Query: 77 PLSINNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGLSKVTLTNTNLQINEILYTPSKSKSDINTEYLRTPNQLYSLM 655
P SI FTI+K I++GA+GKVYLG K K YA+K+V K +M NKN+ HQ+ AER+ALA+S P++VHLYYSLQ+ N+YL+MEYL GGD+K+L+ + G + A Y E+ +AL+YLH H IIHRDLKPDN+LI+N+GH+KLTDFGLSKVTL N ++ + +IL TPS SK +Y RTP Q+ SL+
Sbjct: 28 PPSIEEFTIVKPISRGAFGKVYLGQKGG---KLYAVKVVKKADMINKNMTHQVQAERDALALSKSPFVVHLYYSLQSASNIYLIMEYLIGGDVKSLLHIYGYFDEEMAIKYISEVALALDYLHRHGIIHRDLKPDNMLISNEGHIKLTDFGLSKVTL-NRDINMMDILTTPSMSKP--KQDYSRTPGQVLSLI 214 HSP 2 Score: 112.464 bits (280), Expect = 9.073e-25 Identity = 59/139 (42.45%), Postives = 82/139 (58.99%), Query Frame = 2
Query: 1082 NDGRVLGTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNITKINIDWPDFDLDLS--AVEVINGLLLYDTSKRWGFAQL------NSSDYIDYMRNCSDPPFIPNIAGNDDTGYHDFRN 1474
+DGR+LGTPDYL PE+L+ + + GP +DWWALGV +FE LTGI PF+ ET +++F NI K +I WP+ + LS A ++ LL D SKR G +L + D+ + PF+P DT Y + RN
Sbjct: 720 DDGRILGTPDYLAPELLLGTAH---GPAVDWWALGVCLFEFLTGIPPFNDETPQQVFQNILKRDIPWPEGEEKLSDNAQSAMDMLLTIDDSKRAGMRELKQHPLFSEVDWENLQHQTM--PFVPQPDDETDTSYFEARN 853
BLAST of Serine/threonine-protein kinase greatwall vs. UniProt/SwissProt
Match: sp|Q96GX5|GWL_HUMAN (Serine/threonine-protein kinase greatwall OS=Homo sapiens OX=9606 GN=MASTL PE=1 SV=1) HSP 1 Score: 219.55 bits (558), Expect = 2.842e-61 Identity = 110/193 (56.99%), Postives = 144/193 (74.61%), Query Frame = 2
Query: 77 PLSINNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGLSKVTLTNTNLQINEILYTPSKSKSDINTEYLRTPNQLYSLM 655
P SI F+I+K I++GA+GKVYLG K K YA+K+V K +M NKN+ HQ+ AER+ALA+S P+IVHLYYSLQ+ NVYLVMEYL GGD+K+L+ + G + A Y E+ +AL+YLH H IIHRDLKPDN+LI+N+GH+KLTDFGLSKVTL N ++ + +IL TPS +K +Y RTP Q+ SL+
Sbjct: 29 PPSIEEFSIVKPISRGAFGKVYLGQKGG---KLYAVKVVKKADMINKNMTHQVQAERDALALSKSPFIVHLYYSLQSANNVYLVMEYLIGGDVKSLLHIYGYFDEEMAVKYISEVALALDYLHRHGIIHRDLKPDNMLISNEGHIKLTDFGLSKVTL-NRDINMMDILTTPSMAKP--RQDYSRTPGQVLSLI 215 HSP 2 Score: 112.464 bits (280), Expect = 8.779e-25 Identity = 68/181 (37.57%), Postives = 94/181 (51.93%), Query Frame = 2
Query: 956 HFKTPERKHPMKASTPLRKKQLNLNDKLPSRSSDVLHYLDLTNDGRVLGTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNITKINIDWPDFDLDLS--AVEVINGLLLYDTSKRWGFAQL------NSSDYIDYMRNCSDPPFIPNIAGNDDTGYHDFRN 1474
H +TP + +K+ TP R + V + +DGR+LGTPDYL PE+L+ R GP +DWWALGV +FE LTGI PF+ ET +++F NI K +I WP+ + LS A + LL D +KR G +L + D+ + PFIP DT Y + RN
Sbjct: 707 HQQTPNQ---IKSGTPYRTPK------------SVRRGVAPVDDGRILGTPDYLAPELLLG---RAHGPAVDWWALGVCLFEFLTGIPPFNDETPQQVFQNILKRDIPWPEGEEKLSDNAQSAVEILLTIDDTKRAGMKELKRHPLFSDVDWENLQHQTM--PFIPQPDDETDTSYFEARN 867
BLAST of Serine/threonine-protein kinase greatwall vs. UniProt/SwissProt
Match: sp|E1BFR5|GWL_BOVIN (Serine/threonine-protein kinase greatwall OS=Bos taurus OX=9913 GN=MASTL PE=3 SV=1) HSP 1 Score: 219.164 bits (557), Expect = 3.563e-61 Identity = 112/195 (57.44%), Postives = 146/195 (74.87%), Query Frame = 2
Query: 77 PLSINNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGLSKVTLTNTNLQIN--EILYTPSKSKSDINTEYLRTPNQLYSLM 655
P SI FTI+K I++GA+GKVYLG K + + YA+K+V K +M NKN+ HQ+ AER+ALA+S P+IVHLYYSLQ+ NVYLVMEYL GGD+K+L+ + G + A Y E+ +AL+YLH H IIHRDLKPDN+LI+N+GH+KLTDFGLSKVTL N ++ IN +IL TPS +K +Y RTP Q+ SL+
Sbjct: 29 PPSIEEFTIVKPISRGAFGKVYLGQKGN---RLYAVKVVKKADMINKNMTHQVQAERDALALSKSPFIVHLYYSLQSANNVYLVMEYLIGGDVKSLLHIYGYFDEEMAVKYISEVALALDYLHRHGIIHRDLKPDNMLISNEGHIKLTDFGLSKVTL-NRDIDINMMDILTTPSMAKP--RQDYSRTPGQVLSLI 217 HSP 2 Score: 111.694 bits (278), Expect = 1.485e-24 Identity = 70/192 (36.46%), Postives = 99/192 (51.56%), Query Frame = 2
Query: 923 ENENEDVQFQNHFKTPERKHPMKASTPLRKKQLNLNDKLPSRSSDVLHYLDLTNDGRVLGTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNITKINIDWPDFDLDLS--AVEVINGLLLYDTSKRWGFAQL------NSSDYIDYMRNCSDPPFIPNIAGNDDTGYHDFRN 1474
+ E D+ +Q +TP + +K+ TP R + P +DGR+LGTPDYL PE+L+ R GP +DWWALGV +FE LTGI PF+ ET +++F NI K +I WP+ + LS A ++ LL D +KR G +L + D+ + PFIP DT Y + RN
Sbjct: 703 QRERSDMPYQ---QTPNQ---VKSETPYRTPKSVRRGAAP------------VDDGRILGTPDYLAPELLL---ARAHGPAVDWWALGVCLFEFLTGIPPFNDETPQQVFQNILKRDIPWPEGEEKLSDNAQSAVDILLTIDDTKRAGMKELKHHPLFSGVDWENLQHQ--KMPFIPQPDDETDTSYFEARN 871
BLAST of Serine/threonine-protein kinase greatwall vs. TrEMBL
Match: L7M301 (Putative microtubule-associated serine/threonine kinase OS=Rhipicephalus pulchellus OX=72859 PE=2 SV=1) HSP 1 Score: 283.493 bits (724), Expect = 1.418e-86 Identity = 167/472 (35.38%), Postives = 254/472 (53.81%), Query Frame = 2
Query: 83 SINNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGLSKVTLTNTNLQINEILYTPSKSKSDINTEYLRTPNQLYSLMDKFELCNTMKTHVFSSKRIPQTEWKVKQSHRRKSDVLGDSWDTFISANNRNFYEDKQNSLASHVKNESEILSGIRNCSLDVKSPSKLKQENENEDVQFQNHFKTPERKHPMKASTPLRKKQLNLNDKLPSRSSDVLHYLDLTNDGRVLGTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNITKINIDWP--DFDLDLSAVEVINGLLLYDTSKRWGFAQLNSSDYIDYMRNCS----DPPFIPNIAGNDDTGYHDFRNQI 1480
SI++F ++K I++GA+GKVYL KK + +AIK V K EM +KN+ Q+ AERNALA+S P++VHL+Y LQT V+LVMEY+ GGD+K+L+ G A Y+ E +AL+YLH IIHRDLKPDN+LI+ +GH+KLTDFGLS+V +L +++ TP+ S RTP QL SL ++ + +RIP+ S +R S + + N + + D +S +S L N V SP + +++V F + P P+ ++ + ++ ++ R+LGTPDYL PE+L+ K+ G P+DWWALG+ ++E +TG+ PF ++ E +F NI ++WP + L +AV ++ LL + R GF +L S + + + S +PPF+PN + DT Y D RNQ+
Sbjct: 8 SIDDFVVLKPISRGAFGKVYLAQKKGSEGQLFAIKTVKKSEMVHKNMVDQVLAERNALAVSRSPFVVHLFYCLQTPTCVHLVMEYMIGGDVKSLLHAYGFFEEPMARFYTAEAALALDYLHRRGIIHRDLKPDNMLISAEGHVKLTDFGLSQVECKKIDLA--DLIGTPASRAS--RPVQGRTPGQLLSLTSDLSFTGPQRS---ARRRIPRGSCSAGGSTKRPS-------SSPLVVNAKRLHFDLGSSPPLRSPLDSSSLQSPNN----VLSPEWADGASHSDEV-FTSR---PADTTPVCSAGSMERQTPKVS----------------RGQHRLLGTPDYLAPELLLGQKH---GAPVDWWALGICLYEFMTGLPPFCDQSPEAVFANILHGELEWPIGEEALSPAAVHAVSELLTREPEARPGFQELCSLPFFESVTWSSLLDKEPPFVPNPDDDTDTCYFDARNQM 438
BLAST of Serine/threonine-protein kinase greatwall vs. TrEMBL
Match: A0A293M3F5 (Non-specific serine/threonine protein kinase (Fragment) OS=Ornithodoros erraticus OX=265619 PE=4 SV=1) HSP 1 Score: 280.796 bits (717), Expect = 2.193e-85 Identity = 169/481 (35.14%), Postives = 253/481 (52.60%), Query Frame = 2
Query: 83 SINNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGLSKVTLTNTNLQINEILYTPSKSKSDINTEYL-RTPNQLYSLMDKFELCNTMKTHVFSSKRIPQTEWKVKQSHRRKSDVLGDSWDTFISANNRNFYEDKQNSLASHVKNESEILSGIRNCSLDVKSPSK--LKQENENEDVQFQNHFKTPERKHPMKASTPLRKKQLNLNDKLPSRSSDVLHYLDLTNDGRVLGTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNITKINIDWPDFD--LDLSAVEVINGLLLYDTSKRWGFAQLNSS---DYIDYMRNCSDP-PFIPNIAGNDDTGYHDFRNQIFEYSSN 1498
SI +F+IIK I+KGA+GKV+L KK + +A+K+V K EM NKN+ Q+ AERNALA S P+IV ++Y LQT N+YLVMEY+ GGD+K+L+ + + A+ Y+ ++ +ALEYLH H I+HRDLKPDN+LI++ GH+KLTDFGLS V + +Q +I+ TP + T L RTP QL SL K + +P T + Q R +++ R ++ S + ++ ++ LD+ SPS L +++D F+ T + + ++ + G +LGTPDYL PE+L+ + G +DWW+LGV ++E LTG+ PF ET E +F NI + +++WP+ + L +A + LL D S R +L S +D+ + P PF+P + DTGY + RNQI S N
Sbjct: 32 SIEDFSIIKPISKGAFGKVFLARKKGRDAQVFAVKVVKKSEMVNKNMVGQVFAERNALATSQSPFIVQIFYCLQTVHNIYLVMEYMIGGDVKSLLHVYNYFTEPMATFYAAQVALALEYLHGHGIVHRDLKPDNMLISSTGHIKLTDFGLSHVQV--KKIQPMDIVRTP-----NCGTRLLARTPGQLLSLTSGLHFSGRRK------RSLPDTPSAISQHER------------IVASPTRTPGRCQKRSFLEAASPPARMIRKVKRLQLDLGSPSTDGLNSRVDHDDDPFEEVSGTGKDTYGASVAS--------------------------LSSGHLLGTPDYLAPELLLGQPH---GSAVDWWSLGVCLYEFLTGVPPFCDETPEAVFNNILESDLEWPEGEECLTEAATSAVEALLTRDPSLRASSQELRSMLLFSQVDWDKLHEAPSPFVPCPHDDTDTGYFEARNQILFGSDN 458
BLAST of Serine/threonine-protein kinase greatwall vs. TrEMBL
Match: A0A224Z4G2 (Microtubule associated serine/threonine kinase OS=Rhipicephalus zambeziensis OX=60191 PE=4 SV=1) HSP 1 Score: 276.174 bits (705), Expect = 8.847e-84 Identity = 166/472 (35.17%), Postives = 252/472 (53.39%), Query Frame = 2
Query: 83 SINNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGLSKVTLTNTNLQINEILYTPSKSKSDINTEYLRTPNQLYSLMDKFELCNTMKTHVFSSKRIPQTEWKVKQSHRRKSDVLGDSWDTFISANNRNFYEDKQNSLASHVKNESEILSGIRNCSLDVKSPSKLKQENENEDVQFQNHFKTPERKHPMKASTPLRKKQLNLNDKLPSRSSDVLHYLDLTNDGRVLGTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNITKINIDWP--DFDLDLSAVEVINGLLLYDTSKRWGFAQLNSSDYIDYMRNCS----DPPFIPNIAGNDDTGYHDFRNQI 1480
SI++F ++K I++GA+GKVYL KK + +AIK V K EM +KN+ Q+ AERNALA+S P++VHL+Y LQT V+LVMEY+ GGD+K+L+ G A Y+ E +AL+YLH IIHRDLKPDN+LI+ +GH+KLTDFGLS+V +L +++ TP+ S RTP QL SL ++ + +R+P+ S +R S + + N++ + D S S L S +V SP + +++V F + P P ++ L ++ ++ R+LGTPDYL PE+L+ K+ G P+DWWALG+ ++E +TG+ PF ++ E +F NI ++WP + L +AV ++ LL + R GF +L + + + S +PPF+PN + DT Y D RNQ+
Sbjct: 8 SIDDFVVLKPISRGAFGKVYLAHKKGSVGQLFAIKTVKKSEMVHKNMVDQVLAERNALAVSRSPFVVHLFYCLQTPTCVHLVMEYMIGGDVKSLLHAYGFFEEPMARFYTAEAALALDYLHRRGIIHRDLKPDNMLISAEGHVKLTDFGLSQVECKKIDLA--DLMGTPASRAS--RPVQGRTPGQLLSLTSDLSFTGPQRS---ARRRLPRGSCSAGGSMKRPS-------SSPLLVNSKRLHFDLGTSPPLRSPLGSSSLP-----SPNVLSPEWADGASHSDEV-FTSR---PADTAPACSAGSLERRTPKVS----------------RGQHRLLGTPDYLAPELLLGQKH---GAPVDWWALGICLYEFMTGLPPFCDQSPEAVFANILHGELEWPIGEEALSAAAVHAVSELLTREPEARPGFQELCVLPFFESVTWSSLLDKEPPFVPNPDDDTDTCYFDARNQM 437
BLAST of Serine/threonine-protein kinase greatwall vs. TrEMBL
Match: V4C6X5 (Uncharacterized protein (Fragment) OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_115088 PE=4 SV=1) HSP 1 Score: 276.559 bits (706), Expect = 2.381e-83 Identity = 182/501 (36.33%), Postives = 269/501 (53.69%), Query Frame = 2
Query: 83 SINNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGLSKVTLTNTNLQINEILYTPSKSKSDINTEYLRTPNQLYSLMD--KFELCNTMKTHVFSSKRIPQTEWKVKQSHRRKSDVLGDSWDTFISAN-----------------NRNF-YEDKQNSLASHVKNESEILSGIRNCSLDVKSPSKLKQE-------------NENEDVQFQNHFKTPERKHPMKASTPLRKKQLNLNDKLPSRSS-DVLHYLDLTNDGRVLGTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNITKINIDWPDFDLDLS--AVEVINGLLLYDTSKR---WGFAQLNSSDYIDYMRNC-SDPPFIPNIAGNDDTGYHD 1465
SI +F +K I++GA+GKVYLG KK+ K YAIK + K ++ NKNL Q+ ER+ALA SH P++V+LYYSLQ+E+N+YL+MEY+ GGD+K+L+ M G A +Y E+ +ALEYL+++ IIHRDLKPDN+LI+N+GH+KLTDFGLSK+++ NL+ + S +N Y RTP Q+ SL F C+ + P + + DS ++++A+ + +F D++ S+ K +S SG ++ +++PS +Q +E ++ N TP MK TP + N+ K P R+ V + R+LGTPDYL PEIL+ + G +DWWALGV +FE LTGI PF+ +T E +F NI K +I WP+ + +LS A I+ LL D ++R G + +D+ ++P FIP DT Y D
Sbjct: 2 SIEDFVFLKPISRGAFGKVYLGCKKTAPDKVYAIKAMKKSDLFNKNLVKQVTTERDALATSHSPFVVNLYYSLQSEQNIYLIMEYMIGGDVKSLLTMYGYFDEDMAKMYIAEVTLALEYLNDNGIIHRDLKPDNMLISNEGHIKLTDFGLSKISIDVGNLK---------PASSSVN--YHRTPGQILSLKSSLAFVSCSQNLNPANAKHSTPPIQCLTPTLEDSLNWTSTDSDSSYVTAHSSSLSRPISSLAMNADQSASFTISDRKKSITKPEKRDSRFDSGAEVTTVKIQAPSSNQQTIQSTASSSQDSSMDETPQLRLSNESVTPAE---MK-KTPFKTPHKNIM-KTPFRTPKSVRRGPKDEEEERILGTPDYLAPEILMQKPH---GAGVDWWALGVCLFEFLTGIPPFNDQTPELVFQNILKRDIPWPEEEEELSEQAKSAIDELLTMDPAQRPQAQGVKNMALFADVDWEHVLETEPAFIPQPDDELDTTYFD 483
BLAST of Serine/threonine-protein kinase greatwall vs. TrEMBL
Match: A0A1Z5KXU6 (Protein kinase (Fragment) OS=Ornithodoros moubata OX=6938 PE=4 SV=1) HSP 1 Score: 269.626 bits (688), Expect = 3.608e-81 Identity = 170/475 (35.79%), Postives = 254/475 (53.47%), Query Frame = 2
Query: 83 SINNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGLSKVTLTNTNLQINEILYTPSKSKSDINTEYLRTPNQLYSLMDKFELCNTMKTHVFSSKR---IPQTEWKVKQSHRRKSDVLGDSWDTFISANNRNFYEDKQNSLASHVKNESEILSGIRNCSLDVKSPSKLKQENENEDVQFQNHFKTPERKHPMKASTPLRKKQLNLNDKLPSRSSDVLHYLDLTNDGRVLGTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNITKINIDWPDFD--LDLSAVEVINGLLLYDTSKRWGFAQLNS---SDYIDYMRNCSDP-PFIPNIAGNDDTGYHDFRNQI 1480
+I +F+IIK I+KGA+GKV+L K+ + +A+K+V K EM NKN+ Q+ AERNALA+S P+IV ++Y LQT N+YLVMEY+ GGD+K+L+ + L A+ Y+ E+ +ALEYLH I+HRDLKPDN+LI++ GH+KLTDFGLS+V + +Q +I+ TP+ S + + RTP QL SL FS ++ +P T + Q R S T R+F E + + +L CS + P +++ + P + KK+L ++P+ SS R+LGTPDYL PE+L+ + G +DWW+LGV ++E LTG+ PFS +T E +F NI + ++WP+ + L +A + LL D S R G +L S ID+ P PF+P+ + DTGY + +N +
Sbjct: 32 TIEDFSIIKPISKGAFGKVFLARKQGRDDQVFAVKVVKKFEMVNKNMVDQVFAERNALAMSQSPFIVQIFYCLQTVHNIYLVMEYMIGGDVKSLLHVYNFLPEAMATFYAAEVALALEYLHGRNIVHRDLKPDNMLISSGGHIKLTDFGLSQVEV--KKVQPTDIVRTPN-SGTRLQA---RTPGQLLSLTSGL---------YFSGRKERCLPPTPVGILQHERLTS----SPATTPGRPLKRSFIEATSPPSRVMRRAKRLLLDAESPCSGGLSCPLEMEGD-------------------PFDDISDFGKKRLQ---RIPASSS-----------SRLLGTPDYLAPELLLGQPH---GAAVDWWSLGVCLYEFLTGVPPFSDQTPEAVFNNILQAELEWPEGEECLSEAATNAVEALLTRDPSIRAGSRELRSLALFSQIDWDNLHEAPAPFVPHPHDDTDTGYFEVKNDL 451
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Cavefish
Match: mastl (microtubule associated serine/threonine kinase like [Source:NCBI gene;Acc:103045715]) HSP 1 Score: 208.379 bits (529), Expect = 3.894e-58 Identity = 107/211 (50.71%), Postives = 147/211 (69.67%), Query Frame = 2
Query: 77 PLSINNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGLSKVTLTNTNLQINEILYTPSKSKSDINTEYLRTPNQLYSLMDKFELCNTMKTHVFSSKR 709
P SI +FT++K I++GA+GKV+L KKS+ ++ YA+K+V K +M +KN+ Q+ AER+ALA+S P+IV L+YSLQT VYLVMEYL GGD+K+L+ + G + Y E+ +AL+YLH H IIHRDLKPDN+L++N+GH+KLTDFGLSKV L + L + +IL TPS +K +Y RTP Q+ SL+ L T V KR
Sbjct: 20 PPSIEDFTVVKPISRGAFGKVFLARKKSN-SRLYAVKVVKKADMVDKNMSDQMRAERDALALSKSPFIVRLFYSLQTATKVYLVMEYLIGGDVKSLLHIYGYFEEDMSLKYISEVALALDYLHRHGIIHRDLKPDNMLVSNEGHIKLTDFGLSKVKL-DRELSLMDILTTPSLAKP--KKDYFRTPGQVLSLISSLGL----NTPVMEGKR 222 HSP 2 Score: 102.449 bits (254), Expect = 1.900e-22 Identity = 66/181 (36.46%), Postives = 95/181 (52.49%), Query Frame = 2
Query: 992 ASTPLRKKQLNLNDKL------------PSRSS-DVLHYLDLTNDGRVLGTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNITKINIDWPDFDLDLS--AVEVINGLLLYDTSKRWGFAQLNSSDYI-----DYMRNCSDPPFIPNIAGNDDTGYHDFRN 1474
A TP++KK+ +LN L P R+ V + +LGTPDYL PE+L+ ++ +DWWALGV +FE LTG+ PF+ ET + +F NI +I WPD + +LS A I+ LL D +KR G +L + + D ++N + PFIP DT Y + RN
Sbjct: 721 AMTPVQKKRPSLNSSLYQTPQPMVLSHTPYRTPKSVRRGPEPVVGAPILGTPDYLAPELLLGKQHDFM---VDWWALGVCLFEFLTGVPPFNDETPQLVFQNILNRDIPWPDEEEELSHNARNAIDILLTTDMNKRAGLKELKAHSFFAGLDWDNLQNQAM-PFIPQPDDETDTSYFEARN 897
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Cavefish
Match: mast3b (microtubule associated serine/threonine kinase 3b [Source:ZFIN;Acc:ZDB-GENE-111209-1]) HSP 1 Score: 143.28 bits (360), Expect = 9.045e-36 Identity = 71/160 (44.38%), Postives = 107/160 (66.88%), Query Frame = 2
Query: 89 NNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGLSKVTLTN--TNL 562
++F IK I+ GAYG VYL K R ++ A+K +++Q + +N Q+ ER+ L + P++V ++ S +T +++ +VMEY+ GGD L+ G L + A +Y E ++ALEYLH++ I+HRDLKPDN+LI + GH+KLTDFGLSK+ L N TNL
Sbjct: 380 SDFETIKLISNGAYGAVYLVRHKDTRQRF-AMKKINRQNLILRNQIQQVFVERDILTFAENPFVVSMFCSFETRRHLCMVMEYVEGGDCANLLKNMGPLPVDMARLYFAETVLALEYLHSYGIVHRDLKPDNLLITSMGHIKLTDFGLSKIGLMNMTTNL 538 HSP 2 Score: 103.219 bits (256), Expect = 1.303e-22 Identity = 54/149 (36.24%), Postives = 82/149 (55.03%), Query Frame = 2
Query: 1085 DGRVLGTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNITKINIDWPDFD--LDLSAVEVINGLLLYDTSKRWG------------FAQLNSSDYIDYMRNCSDPPFIPNIAGNDDTGYHDFRNQIFEY 1489
D +V GTP+Y+ PE+++ Y G P+DWWA+G+I++E L G PF G+T EE+FG + +I WPD D L + A ++I LL +R G F +L D+ +R ++ FIP + G DDT Y D R+ + +
Sbjct: 552 DKQVCGTPEYIAPEVILRQGY---GKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVVSDDIIWPDGDDALPVDAQDMITRLLWQSPLERLGTGGATEVKQHTFFTRL---DWNSLLRQKAE--FIPQLEGEDDTSYFDTRSDRYHH 692
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Cavefish
Match: mast2 (microtubule associated serine/threonine kinase 2 [Source:ZFIN;Acc:ZDB-GENE-050309-150]) HSP 1 Score: 138.658 bits (348), Expect = 2.880e-34 Identity = 70/161 (43.48%), Postives = 105/161 (65.22%), Query Frame = 2
Query: 92 NFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGLSKVTLTNTNLQINE 574
+F IK I+ GAYG V+L K R ++ A+K ++KQ + +N Q ER+ L + P++V ++ S +T +++ +VMEY+ GGD TL+ G L + A +Y E ++ALEYLHN+ I+HRDLKPDN+LI + GH+KLTDFGLSK+ L + + E
Sbjct: 428 DFETIKLISNGAYGAVFLVRHKETRQRF-AMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMFCSFETRRHLCMVMEYVEGGDCATLLKHIGALPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHIKLTDFGLSKIGLMSLTTNLYE 587 HSP 2 Score: 92.4337 bits (228), Expect = 3.299e-19 Identity = 51/146 (34.93%), Postives = 78/146 (53.42%), Query Frame = 2
Query: 1085 DGRVLGTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNITKINIDWPDFD--LDLSAVEVINGLLLYDTSKRWGFAQ---------LNSSDYIDYMRNCSDPPFIPNIAGNDDTGYHDFRNQIFEY 1489
D +V GTP+Y+ PE+++ Y G P+DWWA+GVI++E L G PF G+T EE+FG + I WP+ D L A ++I+ LL + +R G D+ +R ++ FIP + DDT Y D R+ + +
Sbjct: 599 DKQVCGTPEYIAPEVILRQGY---GKPVDWWAMGVILYEFLVGCAPFFGDTPEELFGQVISDEIIWPEEDEALPPDAQDLISKLLRQNPLERLGTGSAFEVKQHRFFTDLDWNSLLRQKAE--FIPQLESEDDTSYFDTRSDRYHH 739
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Cavefish
Match: stk38b (serine/threonine kinase 38b [Source:ZFIN;Acc:ZDB-GENE-081031-2]) HSP 1 Score: 129.028 bits (323), Expect = 4.160e-32 Identity = 62/151 (41.06%), Postives = 100/151 (66.23%), Query Frame = 2
Query: 80 LSINNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGL 532
L +++F +K I +GA+G+V L +K D YA+K++ K +M K I AER+ L + ++V ++YS Q ++N+YL+ME+L GGD+ TL++ LS +EA Y E ++A++ +H IHRD+KPDN+L+ + GH+KL+DFGL
Sbjct: 84 LGLDDFESLKVIGRGAFGEVRL-VQKKDTGHVYAMKILRKADMLEKEQVGHIRAERDILVQADSLWVVKMFYSFQDKQNLYLLMEFLPGGDMMTLLMKKDTLSEEEAQFYVAETVLAIDSIHQLGFIHRDIKPDNLLLDSKGHVKLSDFGL 233 HSP 2 Score: 59.6918 bits (143), Expect = 3.762e-9 Identity = 24/56 (42.86%), Postives = 35/56 (62.50%), Query Frame = 2
Query: 1094 VLGTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNI 1261
+GTPDY+ PE+ + + Y DWW+LGVI++EML G PF ET +E + +
Sbjct: 282 TVGTPDYIAPEVFMQTGY---NKLCDWWSLGVIMYEMLIGYPPFCSETPQETYKKV 334
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Cavefish
Match: lats2 (large tumor suppressor kinase 2 [Source:NCBI gene;Acc:103025207]) HSP 1 Score: 131.724 bits (330), Expect = 4.744e-32 Identity = 65/146 (44.52%), Postives = 95/146 (65.07%), Query Frame = 2
Query: 95 FTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGL 532
F IK + GA+G+V L T+K D YA+K + K+++ N+N + AER+ LA + ++V LYYS Q ++Y VM+Y+ GGD+ +L++ G+ Q A Y E+ +A+E +H IHRD+KPDNILI DGH+KLTDFGL
Sbjct: 705 FVKIKTLGIGAFGEVCL-TRKVDTGALYAMKTLRKKDVLNRNQVAHVKAERDILAEADNEWVVRLYYSFQDRDSLYFVMDYIPGGDMMSLLIRMGVFPEQLARFYVAELTLAIESVHKMGFIHRDIKPDNILIDLDGHIKLTDFGL 849 HSP 2 Score: 58.9214 bits (141), Expect = 1.020e-8 Identity = 45/140 (32.14%), Postives = 66/140 (47.14%), Query Frame = 2
Query: 1094 VLGTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNITKIN-IDW-------PDFDLDLSAVEVINGLLLYDTSKRW---GFAQLNSSDY---IDYMRN--CSDPPFIPNIAGNDDTGYHD 1465
++GTP+Y+ PE+L+ Y DWW++GVI+FEML G PF T E T+I I+W P L AV++I G L +R G ++ + + +D+ N P+ P IA DT D
Sbjct: 910 LVGTPNYIAPEVLLRKGYTQL---CDWWSVGVILFEMLVGQPPFLAPTPTE-----TQIKVINWESTLQVPPQVKLSPEAVDII-GRLCCSAEERLGSNGAGEIKAHPFFSQVDFSSNLRTQPAPYRPKIAHPMDTSNFD 1040
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Sea Lamprey
Match: mast2 (microtubule associated serine/threonine kinase 2 [Source:ZFIN;Acc:ZDB-GENE-050309-150]) HSP 1 Score: 145.206 bits (365), Expect = 3.959e-37 Identity = 73/160 (45.62%), Postives = 107/160 (66.88%), Query Frame = 2
Query: 89 NNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGLSKVTLTN--TNL 562
+F IK I+ GAYG VYL K R ++ A+K ++KQ + +N Q ER+ L + P++V ++ S +T++++ +VMEY+ GGD TL+ G L + A +Y E ++ALEYLHN+ I+HRDLKPDN+LI + GH+KLTDFGLSK+ L + TNL
Sbjct: 212 EDFETIKLISNGAYGAVYLVRHKETRQRF-AMKKINKQNLILRNQIQQAFVERDILTFAENPFVVGMFCSFETKRHLCMVMEYVEGGDCATLLKNLGALPVDMAQLYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHVKLTDFGLSKIGLMSLTTNL 370 HSP 2 Score: 95.5153 bits (236), Expect = 5.780e-21 Identity = 49/146 (33.56%), Postives = 79/146 (54.11%), Query Frame = 2
Query: 1085 DGRVLGTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNITKINIDWPDFD--LDLSAVEVINGLLLYDTSKRWGFAQ---------LNSSDYIDYMRNCSDPPFIPNIAGNDDTGYHDFRNQIFEY 1489
D +V GTP+Y+ PE+++ Y G P+DWWA+GVI++E L G PF G+T EE+FG + I WP+ + L ++I GLL + +R G +S D+ +R ++ F+P + +DT Y D R+ + +
Sbjct: 384 DKQVCGTPEYIAPEVILRQGY---GKPVDWWAMGVILYEFLVGCVPFFGDTPEELFGQVISDEIVWPEGEDALPSDVQDLITGLLERNPLERLGTGGAFEVKQHQFFHSLDWDGLLRQKAE--FVPQLESEEDTSYFDTRSDRYHH 524
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Sea Lamprey
Match: ENSPMAT00000003561.1 (pep scaffold:Pmarinus_7.0:GL478057:5187:43749:1 gene:ENSPMAG00000003167.1 transcript:ENSPMAT00000003561.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 120.939 bits (302), Expect = 4.431e-29 Identity = 67/195 (34.36%), Postives = 118/195 (60.51%), Query Frame = 2
Query: 14 FLRSKIIL*VIN---KLLFWILNMPLSINNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGLSKVTLTNTNLQINEILYTP 589
F R+K I +N K + + ++ + N++ ++K I +GA+G+V L K+ + K YA+K++SK EM ++ ER+ +A ++ P++V L+Y+ Q + +Y+VMEY+ GGDL L+ + + A Y+ E+++AL+ +H+ IHRD+KPDN+L+ +GHLKL DFG T ++ + + TP
Sbjct: 46 FFRNKNIDNFLNRYEKAVGKVRDLRMRANDYDVVKVIGRGAFGEVQLVRHKATK-KVYAMKLLSKFEMTKRSDSAFFWEERDIMAFANSPWVVQLFYAFQDHRYLYMVMEYMPGGDLVNLMSNYDVPE-KWARFYTAEVVLALDAIHSMGFIHRDVKPDNMLLDKNGHLKLADFGTCMKMDTEGMVRCDTAVGTP 238 HSP 2 Score: 57.3806 bits (137), Expect = 5.660e-9 Identity = 40/138 (28.99%), Postives = 66/138 (47.83%), Query Frame = 2
Query: 1094 VLGTPDYLPPEILINSKYR-LQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNIT--KINIDWPDFDLDLS--AVEVINGLLLYDTSKRWGFAQLNSSDYIDYMRN---------CSDPPFIPNIAGNDDTGYHD 1465
+GTPDY+ PE+L + G DWW++GV ++EML G TPF +++ + I K +++PD D+D+S A +I L+ D R G + + +N + P +P + + DT D
Sbjct: 234 AVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMDHKNALNFPD-DVDISKDAKNLICAFLI-DREVRVGRNGVGEIKRHPFFKNDQWTWENIRETAAPVVPELVSDTDTSNFD 369
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005485.1 (pep scaffold:Pmarinus_7.0:GL480333:786:25860:1 gene:ENSPMAG00000004915.1 transcript:ENSPMAT00000005485.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 113.62 bits (283), Expect = 7.576e-29 Identity = 61/151 (40.40%), Postives = 94/151 (62.25%), Query Frame = 2
Query: 92 NFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALA--ISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGLSK 538
+F +K I KG++GKV L K+D K+YA+K++ K + + I AERN L + H P++V L++S QT +Y +++Y+ GG+L + S Q A Y+ EI A+ +LH+ I++RDLKP+NIL+ + GH+ LTDFGL K
Sbjct: 100 DFDFLKVIGKGSFGKVLLAKHKTD-GKFYAVKVLQKSAILKRREQKHIMAERNVLLKNVKH-PFLVGLHFSFQTADKLYFILDYVNGGELFLHLQRERCFSEQRARFYAAEIASAIGHLHSLNIVYRDLKPENILLDSKGHIVLTDFGLCK 248
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Sea Lamprey
Match: stk38l (serine/threonine kinase 38 like [Source:ZFIN;Acc:ZDB-GENE-040426-798]) HSP 1 Score: 110.538 bits (275), Expect = 6.827e-27 Identity = 52/128 (40.62%), Postives = 82/128 (64.06%), Query Frame = 2
Query: 149 TKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGL 532
+K D YA+K++ K +M K I AER+ L + ++V ++YS Q KN+YL+ME+L GGD+ TL++ L+ +E Y E ++A++ +H IHRD+KPDN+L+ + GH+KL+DFGL
Sbjct: 4 VQKKDTGHIYAMKVLRKADMLEKEQVAHIRAERDILVEADNLWVVKMFYSFQDPKNLYLIMEFLPGGDMMTLLMKKDTLTEEETQFYIAESILAIDSIHQLGFIHRDIKPDNLLLDSKGHVKLSDFGL 131 HSP 2 Score: 58.9214 bits (141), Expect = 1.108e-9 Identity = 24/56 (42.86%), Postives = 35/56 (62.50%), Query Frame = 2
Query: 1094 VLGTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNI 1261
+GTPDY+ PE+ + + Y DWW+LGVI+FEML G PF E+ +E + +
Sbjct: 182 TVGTPDYIAPEVFLQTGY---NKLCDWWSLGVIMFEMLIGYPPFCSESPQETYRKV 234
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006766.1 (pep scaffold:Pmarinus_7.0:GL478342:9725:27922:-1 gene:ENSPMAG00000006086.1 transcript:ENSPMAT00000006766.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 111.309 bits (277), Expect = 7.319e-27 Identity = 63/158 (39.87%), Postives = 93/158 (58.86%), Query Frame = 2
Query: 71 NMPLSINNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALA--ISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGLSK 538
N+ ++F + + KG++GKV L KSD +YA+K++ K + K I AERN L + H P++V L+YS Q+ +Y VM+Y+ GG+L + G A Y+ EI A+ YLH+ I++RDLKP+NIL+ GH+ LTDFGL K
Sbjct: 84 NLQAKPSDFDFLTVLGKGSFGKVLLAKHKSDGI-FYAVKVMKKSIILQKKEEKHIMAERNVLVKNVRH-PFLVGLHYSFQSADKLYFVMDYVNGGELFFHLQRQGSFPESRAQFYAAEIGSAIGYLHSLNIVYRDLKPENILLDYQGHIVLTDFGLCK 239 HSP 2 Score: 59.6918 bits (143), Expect = 7.065e-10 Identity = 41/136 (30.15%), Postives = 69/136 (50.74%), Query Frame = 2
Query: 1100 GTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNITKINIDWPDFDLDLSAVEVINGLLLYDTSKRWG-------------FAQLNSSDYIDYMRNCSDPPFIPNIAGNDDTGYHDF 1468
GTP+YL PE+L Y +DWW LG +++EML G+ PF + +++++ +I + + +A+ V+ GLL D +R G F+ +N +D +D R S P+ P ++G D H+F
Sbjct: 253 GTPEYLAPEVLKKHPYDRT---VDWWCLGCVLYEMLYGLPPFYSKNLKQMYESILHQPLQLRPCTTN-AAISVLEGLLHKDPHQRLGATSDILEIKNHVFFSTINWND-LDAKRIPS--PYNPGVSGPCDL--HNF 379
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Yeast
Match: RIM15 (Protein kinase involved in cell proliferation in response to nutrients; glucose-repressible; involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; identified as a regulator of IME2; phosphorylates Igo1p and Igo2p; substrate of Pho80p-Pho85p kinase [Source:SGD;Acc:S000001861]) HSP 1 Score: 145.976 bits (367), Expect = 1.516e-37 Identity = 82/185 (44.32%), Postives = 109/185 (58.92%), Query Frame = 2
Query: 44 INKLLFWILNMPLS----------------INNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAI-SHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGLSKVTL 547
IN + NMPLS I ++ I+K I+KGAYG VYL KK Y+AIK++ K +M KN + +ER + + S PY+ L+ S Q + N++LVMEYL GGDL TLI M G L Q A Y EI++ + +H + IIH DLKP+N+LI N GH+KLTDFGLS+ L
Sbjct: 761 INNAMLTSPNMPLSPLLLATNQTVKSPTPSIKDYDILKPISKGAYGSVYLARKKL-TGDYFAIKVLRKSDMIAKNQVTNVKSERAIMMVQSDKPYVARLFASFQNKDNLFLVMEYLPGGDLATLIKMMGYLPDQWAKQYLTEIVVGVNDMHQNGIIHHDLKPENLLIDNAGHVKLTDFGLSRAGL 944
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Yeast
Match: CBK1 (Serine/threonine protein kinase of the the RAM signaling network; Ndr/LATS family member; binds regulatory subunit Mob2p; involved in regulation of cellular morphogenesis, polarized growth, and septum destruction; phosphorylation by Cbk1p regulates localization and activity of Ace2p transcription factor and Ssd1p translational repressor; Cbk1p activity is regulated by both phosphorylation and specific localization; relocalizes to cytoplasm upon DNA replication stress [Source:SGD;Acc:S000005105]) HSP 1 Score: 133.265 bits (334), Expect = 1.427e-33 Identity = 67/148 (45.27%), Postives = 95/148 (64.19%), Query Frame = 2
Query: 92 NFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGLS 535
+F +K I KGA+G+V L +K D K YA+K + K EM K+ + AER+ LA S P++V LYYS Q + +YL+ME+L GGDL T+++ + + Y E ++A+E +H IHRD+KPDNILI GH+KL+DFGLS
Sbjct: 351 DFHTVKVIGKGAFGEVRL-VQKKDTGKIYAMKTLLKSEMYKKDQLAHVKAERDVLAGSDSPWVVSLYYSFQDAQYLYLIMEFLPGGDLMTMLIRWQLFTEDVTRFYMAECILAIETIHKLGFIHRDIKPDNILIDIRGHIKLSDFGLS 497 HSP 2 Score: 61.2326 bits (147), Expect = 2.234e-10 Identity = 44/134 (32.84%), Postives = 72/134 (53.73%), Query Frame = 2
Query: 1094 VLGTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNITKI--NIDWPDFDLDLS--AVEVINGLLLYDTSK--RWGFA-QLNSSDY---IDY--MRNCSDPPFIPNIAGNDDTGY 1459
+GTPDY+ PEI + Y+ G DWW+LG I++E L G PF ET +E + I + +PD D+ +S A ++I LL + + R G A ++ S + +D+ +R + P+IP ++ DT +
Sbjct: 571 TVGTPDYIAPEIFL---YQGYGQECDWWSLGAIMYECLIGWPPFCSETPQETYRKIMNFEQTLQFPD-DIHISYEAEDLIRRLLTHADQRLGRHGGADEIKSHPFFRGVDWNTIRQV-EAPYIPKLSSITDTRF 699
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Yeast
Match: TPK2 (cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk3p; localizes to P-bodies during stationary phase; relocalizes to the cytosol in response to hypoxia [Source:SGD;Acc:S000006124]) HSP 1 Score: 125.561 bits (314), Expect = 2.624e-32 Identity = 57/158 (36.08%), Postives = 101/158 (63.92%), Query Frame = 2
Query: 83 SINNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGLSKVTLTNT 556
++++F I++ + G++G+V+L + +YYAIK++ KQ++ N ER L + P+++ ++ + Q +N+++VM+Y+ GG+L +L+ A Y+ E+++ALEYLH H II+RDLKP+NIL+ +GH+K+TDFG +K T T
Sbjct: 66 TLHDFQIMRTLGTGSFGRVHL-VRSVHNGRYYAIKVLKKQQVVKMKQVEHTNDERRMLKLVEHPFLIRMWGTFQDARNIFMVMDYIEGGELFSLLRKSQRFPNPVAKFYAAEVILALEYLHAHNIIYRDLKPENILLDRNGHIKITDFGFAKEVQTVT 222 HSP 2 Score: 64.3142 bits (155), Expect = 1.289e-11 Identity = 33/95 (34.74%), Postives = 53/95 (55.79%), Query Frame = 2
Query: 1100 GTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNITKINIDWPDFDLDLSAVEVINGLLLYDTSKRWGFAQLNSSD 1384
GTPDY+ PE++ Y +DWW+LGV+I+EML G TPF T + + I + + +P + V++++ L+ D ++R G Q S D
Sbjct: 227 GTPDYIAPEVITTKPYNKS---VDWWSLGVLIYEMLAGYTPFYDTTPMKTYEKILQGKVVYPPY-FHPDVVDLLSKLITADLTRRIGNLQSGSRD 317
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Yeast
Match: YPK1 (S/T protein kinase; phosphorylates, downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p by phosphorylation in response to compromised sphingolipid synthesis; involved in the TORC-dependent phosphorylation of ribosomal proteins Rps6a/b (S6); mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; human homolog SGK1 can complement a null mutant; human homolog SGK2 can complement a ypk1 ypk2 double mutant [Source:SGD;Acc:S000001609]) HSP 1 Score: 126.716 bits (317), Expect = 1.738e-31 Identity = 63/161 (39.13%), Postives = 99/161 (61.49%), Query Frame = 2
Query: 71 NMPLSINNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGLSKVTLTN 553
N PLSI++F ++K I KG++GKV + +K D K YA+K + K + +K+ AER LA CP+IV L +S Q+ + +Y V+ ++ GG+L + G L A Y+ E++ AL+ LH +++RDLKP+NIL+ GH+ L DFGL K+ + +
Sbjct: 339 NKPLSIDDFDLLKVIGKGSFGKV-MQVRKKDTQKVYALKAIRKSYIVSKSEVTHTLAERTVLARVDCPFIVPLKFSFQSPEKLYFVLAFINGGELFYHLQKEGRFDLSRARFYTAELLCALDNLHKLDVVYRDLKPENILLDYQGHIALCDFGLCKLNMKD 498 HSP 2 Score: 70.4774 bits (171), Expect = 2.289e-13 Identity = 45/135 (33.33%), Postives = 72/135 (53.33%), Query Frame = 2
Query: 1100 GTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNITKINIDWPD-FDLDLSAVEVINGLLLYDTSKRWGFAQLNSSDYI------------DYMRNCSDPPFIPNIAGNDDTGYHD 1465
GTP+YL PE+L+ Y +DWW LGV+++EMLTG+ P+ E V +++ I + + +PD FD D A +++ GLL D ++R G+ N +D I + PP+ P ++ + DT D
Sbjct: 507 GTPEYLAPELLLGLGYT---KAVDWWTLGVLLYEMLTGLPPYYDEDVPKMYKKILQEPLVFPDGFDRD--AKDLLIGLLSRDPTRRLGY---NGADEIRNHPFFSQLSWKRLLMKGYIPPYKPAVSNSMDTSNFD 633
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Yeast
Match: YPK2 (Protein kinase similar to S/T protein kinase Ypk1p; functionally redundant with YPK1 at the genetic level; participates in a signaling pathway required for optimal cell wall integrity; involved in the TORC-dependent phosphorylation of ribosomal proteins Rps6a/b (S6); human homolog SGK2 can complement a ypk1 ypk2 double mutant [Source:SGD;Acc:S000004710]) HSP 1 Score: 125.561 bits (314), Expect = 4.447e-31 Identity = 65/163 (39.88%), Postives = 100/163 (61.35%), Query Frame = 2
Query: 71 NMPLSINNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGLSKVTLTNTN 559
N PLSI++F ++K I KG++GKV + +K D K YA+K + K + +K AER LA CP+IV L +S Q+ + +YLV+ ++ GG+L + G SL + Y E++ AL+ LH +I+RDLKP+NIL+ GH+ L DFGL K+ + + +
Sbjct: 336 NKPLSIDDFDLLKVIGKGSFGKV-MQVRKKDTQKIYALKALRKAYIVSKCEVTHTLAERTVLARVDCPFIVPLKFSFQSPEKLYLVLAFINGGELFYHLQHEGRFSLARSRFYIAELLCALDSLHKLDVIYRDLKPENILLDYQGHIALCDFGLCKLNMKDND 497 HSP 2 Score: 70.8626 bits (172), Expect = 1.916e-13 Identity = 44/134 (32.84%), Postives = 71/134 (52.99%), Query Frame = 2
Query: 1100 GTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNITKINIDWPDFDLDLSAVEVINGLLLYDTSKRWGFAQLNSSDYI---DYMRNCS---------DPPFIPNIAGNDDTGYHD 1465
GTP+YL PEIL+ Y +DWW LG++++EM+TG+ P+ E V ++ I + + +PD D +A +++ GLL D S+R G +N +D I + ++ S PP+ P + DT D
Sbjct: 504 GTPEYLAPEILLGQGYTKT---VDWWTLGILLYEMMTGLPPYYDENVPVMYKKILQQPLLFPD-GFDPAAKDLLIGLLSRDPSRRLG---VNGTDEIRNHPFFKDISWKKLLLKGYIPPYKPIVKSEIDTANFD 630
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Nematostella
Match: EDO38489 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SCT2]) HSP 1 Score: 199.904 bits (507), Expect = 1.767e-61 Identity = 97/197 (49.24%), Postives = 138/197 (70.05%), Query Frame = 2
Query: 86 INNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGLSKVTLTNTNLQINEILYTPS----KSKSDINTEYLRTPNQLYSLMDKF 664
I++F IIK I++GA+G+VYLG K+ + YAIK++ K+++ NKN+ Q+ AER+ALAIS PY+VHL+YS Q+ ++LVMEYL GGDLK+L+ G + A Y+ +I++ALEYLH+H IIHRDLKPDN+L++N G +KLTDFGLS++T +++ TPS +S+S Y RTP Q+ SL F
Sbjct: 1 IDDFDIIKPISRGAFGQVYLGRKRDKSGQLYAIKVMKKKDVINKNMIEQVVAERDALAISKSPYVVHLFYSFQSRDKIFLVMEYLVGGDLKSLLHNIGYFDEEMARFYTAQIILALEYLHSHGIIHRDLKPDNMLLSNTGKVKLTDFGLSQLT-RERKPSFQDVMSTPSVATPRSQSQRRPAYHRTPGQILSLTSNF 196
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Nematostella
Match: EDO42523 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S134]) HSP 1 Score: 144.436 bits (363), Expect = 4.297e-37 Identity = 71/162 (43.83%), Postives = 108/162 (66.67%), Query Frame = 2
Query: 89 NNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGLSKVTLTNTNLQINE 574
+F IK I+ GA+G VYL K T++ AIK ++K M +KN Q+ AER+ L + P++V L+ +T+K++ +VMEY+ GGD +L+ G L A +Y E ++A+EYLH++ I+HRD+KPDN+LI + GH+KLTDFGLSK+ L N+ ++ E
Sbjct: 286 EDFDYIKLISNGAFGAVYLVRHKETHTRF-AIKKINKHAMLHKNQVEQVFAERDILTFAENPFVVGLWCCFETKKHLCMVMEYVEGGDCASLLKNIGALPADLARMYFAETVLAVEYLHSYGIVHRDIKPDNLLITSLGHIKLTDFGLSKIGLMNSTTRMYE 446 HSP 2 Score: 96.2857 bits (238), Expect = 4.809e-21 Identity = 54/148 (36.49%), Postives = 82/148 (55.41%), Query Frame = 2
Query: 1085 DGRVLGTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNITKINI---DWPDFDLDLSAVEVINGLLLYDTSKRWGFAQ---------LNSSDYIDYMRNCSDPPFIPNIAGNDDTGYHDFRNQIFEYS 1492
D +V GTPDYL PE+++ Y G +DWW++G+I++E L G+ PF G+T EE+F + +I D D D AV++I GLL D ++R G A D+ +R ++ FIP + G DDT Y D R++ + +S
Sbjct: 458 DKQVFGTPDYLAPEVILRQGY---GRAVDWWSMGIILYEFLMGVPPFYGDTPEELFAHTLSGDIEGLDGEDAPPD-DAVDLIRGLLEQDPTQRLGSAGALEVKEHVFFTGLDWTALLRQKAE--FIPQLEGEDDTSYFDSRSERYNHS 599
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Nematostella
Match: EDO28503 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T6A2]) HSP 1 Score: 138.658 bits (348), Expect = 1.175e-36 Identity = 71/167 (42.51%), Postives = 109/167 (65.27%), Query Frame = 2
Query: 89 NNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKN-----LFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGLSKVTLTNTNLQINE 574
+F IK I+ GA+G VYL K T++ AIK ++K M +KN + Q+ AER+ L + P++V L+ +T+K++ +VMEY+ GGD +L+ G L A +Y E ++A+EYLH++ I+HRD+KPDN+LI + GH+KLTDFGLSK+ L N+ ++ E
Sbjct: 99 EDFDYIKLISNGAFGAVYLVRHKETHTRF-AIKKINKHAMLHKNQVVISMLKQVFAERDILTFAENPFVVGLWCCFETKKHLCMVMEYVEGGDCASLLKNIGALPADLARMYFAETVLAVEYLHSYGIVHRDIKPDNLLITSLGHIKLTDFGLSKIGLMNSTTRMYE 264 HSP 2 Score: 73.9442 bits (180), Expect = 2.546e-14 Identity = 38/89 (42.70%), Postives = 55/89 (61.80%), Query Frame = 2
Query: 1085 DGRVLGTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNITKINI---DWPDFDLDLSAVEVINGLLLYD 1342
D +V GTPDYL PE+++ Y G +DWW++G+I++E L G+ PF G+T EE+F + +I D D D AV++I GLL D
Sbjct: 276 DKQVFGTPDYLAPEVILRQGY---GRAVDWWSMGIILYEFLMGVPPFYGDTPEELFAHTLSGDIEGLDGEDAPPD-DAVDLIRGLLEQD 360
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Nematostella
Match: EDO36034 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SJR1]) HSP 1 Score: 134.42 bits (337), Expect = 1.474e-34 Identity = 64/151 (42.38%), Postives = 101/151 (66.89%), Query Frame = 2
Query: 80 LSINNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGL 532
+ I+ F I+ I GA+G+V+L +K+D + YA+K++ K E+ +N + AER+ LA + ++V LYYS Q ++N+Y VM+Y+ GGDL +L++ G+ A Y E+++A++ +H +HRD+KPDNILI DGH+KLTDFGL
Sbjct: 33 MDISMFEKIRTIGIGAFGEVWL-VRKTDTSMLYAMKILRKSEVFRRNQAAHVKAERDILAEADNEWVVKLYYSFQDQENLYFVMDYVPGGDLMSLLIKFGIFPEDYAKFYIAELVLAIDSVHRMGFVHRDIKPDNILIDKDGHIKLTDFGL 182 HSP 2 Score: 54.299 bits (129), Expect = 6.786e-8 Identity = 23/51 (45.10%), Postives = 33/51 (64.71%), Query Frame = 2
Query: 1094 VLGTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEE 1246
++GTP+Y+ PE+L+ YR DWW++GVI++EML G PF T E
Sbjct: 253 LVGTPNYIAPEVLLRIGYRQS---CDWWSVGVILYEMLVGQPPFLAPTPAE 300
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Nematostella
Match: EDO37800 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SEW2]) HSP 1 Score: 121.324 bits (303), Expect = 3.591e-30 Identity = 64/152 (42.11%), Postives = 98/152 (64.47%), Query Frame = 2
Query: 89 NNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALA--ISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGLSK 538
++F +K I KG++GKV+L +K K+YAIK+++K ++ +N I AERN L ++H P++V L+YS QT+ +Y V++Y+ GG+L + A Y+ EI A+ YLH +II+RDLKP+NIL+ + GH+ LTDFGL K
Sbjct: 72 SDFLFLKVIGKGSFGKVFL-SKNKQEDKFYAIKVLNKSAIRKRNEAKHIMAERNVLVQNVTH-PFLVGLHYSFQTKDKLYFVLDYVNGGELFFHLQRERYFPESRARFYAAEIASAIGYLHGLQIIYRDLKPENILLDSKGHIVLTDFGLCK 221 HSP 2 Score: 67.3958 bits (163), Expect = 4.443e-12 Identity = 39/133 (29.32%), Postives = 64/133 (48.12%), Query Frame = 2
Query: 1100 GTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNITKINIDWPDFDLDLSAVEVINGLLLYDTSKRWGFAQ-----------LNSSDYIDYMRNCSDPPFIPNIAGNDDTGYHD 1465
GTP+YL PE+L Y +DWW LG +++EM+ G+ PF E++ NI + ++ SA ++ GLL D +KR G + S ++ + +PP+ PN++G D + D
Sbjct: 235 GTPEYLAPEVLRKQDYDKS---VDWWCLGAVLYEMMYGLPPFYSRDTAEMYDNILHKPLRLRT-NISQSARHILEGLLQKDKTKRLGSGEGDFEDIRTHSFFRSINWQQLVARGIEPPYNPNVSGQLDLKHFD 363
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Medaka
Match: mastl (microtubule associated serine/threonine kinase like [Source:NCBI gene;Acc:101170130]) HSP 1 Score: 207.994 bits (528), Expect = 2.476e-58 Identity = 103/196 (52.55%), Postives = 142/196 (72.45%), Query Frame = 2
Query: 77 PLSINNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGLSKVTLTNTNLQINEILYTPSKSKSDINTEYLRTPNQLYSLMDKF 664
P SI++F ++K I++GA+GKVYL KKS+ + YAIK++ K +M +KN+ +Q+ AER+ALA+S P++VHL+YSLQT +YLVMEYL GGD+K+L+ + G A Y E+ +AL+YLH H IIHRDLKPDN+LI+N GH+KLTDFGLSKV L + L + +IL TPS +K +Y RTP Q+ SL+
Sbjct: 21 PPSIDDFVVLKPISRGAFGKVYLARKKSN-ARLYAIKVMKKADMVDKNMTNQMKAERDALALSKSPFVVHLFYSLQTATKIYLVMEYLIGGDVKSLLHIYGYFDQDMAVKYISEVALALDYLHRHGIIHRDLKPDNMLISNVGHIKLTDFGLSKVKL-DRELSLMDILTTPSLAKP--KKDYFRTPGQVLSLISSL 212 HSP 2 Score: 95.9005 bits (237), Expect = 2.032e-20 Identity = 62/191 (32.46%), Postives = 93/191 (48.69%), Query Frame = 2
Query: 941 VQFQNHFKTPERKHPMKASTPLRKKQLNLNDKLPSRSSDVLHYLDLTNDGR-VLGTPDYLPPEILINSKY-RLQGPP---IDWWALGVIIFEMLTGITPFSGETVEEIFGNITKINIDWPDFDLDLSA--VEVINGLLLYDTSKRWGFAQLNSS------DYIDYMRNCSDPPFIPNIAGNDDTGYHDFRN 1474
+ + TP ++ P +S + + P R+ + +G +LGTPDYL PE+L+ + +GP +DWWALGV +FE LTG+ PF+ ET + +F NI +I WP+ D +LSA I LL D ++R L S D+++ + PFIP DT Y + RN
Sbjct: 626 ASYHSGAATPVQRRPYSSSPAHQTPRPTSTSHTPFRTPKSVRRGVAPAEGAPILGTPDYLAPELLLGKPHVSGKGPSDCMVDWWALGVCLFEFLTGVPPFNDETPQLVFQNILNRDIPWPEGDEELSANSRNAIEILLTMDMTRRASLKDLESHPLFAGLDWVNLQNQ--EMPFIPQPEDETDTSYFEARN 814
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Medaka
Match: mastl (microtubule associated serine/threonine kinase like [Source:NCBI gene;Acc:101170130]) HSP 1 Score: 207.994 bits (528), Expect = 2.505e-58 Identity = 103/196 (52.55%), Postives = 142/196 (72.45%), Query Frame = 2
Query: 77 PLSINNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGLSKVTLTNTNLQINEILYTPSKSKSDINTEYLRTPNQLYSLMDKF 664
P SI++F ++K I++GA+GKVYL KKS+ + YAIK++ K +M +KN+ +Q+ AER+ALA+S P++VHL+YSLQT +YLVMEYL GGD+K+L+ + G A Y E+ +AL+YLH H IIHRDLKPDN+LI+N GH+KLTDFGLSKV L + L + +IL TPS +K +Y RTP Q+ SL+
Sbjct: 21 PPSIDDFVVLKPISRGAFGKVYLARKKSN-ARLYAIKVMKKADMVDKNMTNQMKAERDALALSKSPFVVHLFYSLQTATKIYLVMEYLIGGDVKSLLHIYGYFDQDMAVKYISEVALALDYLHRHGIIHRDLKPDNMLISNVGHIKLTDFGLSKVKL-DRELSLMDILTTPSLAKP--KKDYFRTPGQVLSLISSL 212 HSP 2 Score: 95.9005 bits (237), Expect = 1.691e-20 Identity = 60/187 (32.09%), Postives = 90/187 (48.13%), Query Frame = 2
Query: 941 VQFQNHFKTPERKHPMKASTPLRKKQLNLNDKLPSRSSDVLHYLDLTNDGR-VLGTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNITKINIDWPDFDLDLSA--VEVINGLLLYDTSKRWGFAQLNSS------DYIDYMRNCSDPPFIPNIAGNDDTGYHDFRN 1474
+ + TP ++ P +S + + P R+ + +G +LGTPDYL PE+L+ + +DWWALGV +FE LTG+ PF+ ET + +F NI +I WP+ D +LSA I LL D ++R L S D+++ + PFIP DT Y + RN
Sbjct: 626 ASYHSGAATPVQRRPYSSSPAHQTPRPTSTSHTPFRTPKSVRRGVAPAEGAPILGTPDYLAPELLLGKPHDCM---VDWWALGVCLFEFLTGVPPFNDETPQLVFQNILNRDIPWPEGDEELSANSRNAIEILLTMDMTRRASLKDLESHPLFAGLDWVNLQNQ--EMPFIPQPEDETDTSYFEARN 807
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Medaka
Match: mast3b (microtubule associated serine/threonine kinase 3 [Source:NCBI gene;Acc:101161044]) HSP 1 Score: 144.821 bits (364), Expect = 2.828e-36 Identity = 71/160 (44.38%), Postives = 108/160 (67.50%), Query Frame = 2
Query: 89 NNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGLSKVTLTN--TNL 562
++F +K I+ GAYG VYL K R ++ A+K +++Q + +N Q ER+ L + P++V ++ S +T +++ +VMEY+ GGD TL+ G L ++ + +Y E ++ALEYLHN+ I+HRDLKPDN+LI + GH+KLTDFGLSK+ L N TNL
Sbjct: 347 SDFETMKLISNGAYGAVYLVRHKETRQRF-AMKKINRQNLVLRNQIQQAFVERDILTFAENPFVVSMFCSFETRRHLCMVMEYVEGGDCATLLKNMGPLPVEMSRMYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHIKLTDFGLSKIGLMNMTTNL 505 HSP 2 Score: 97.8265 bits (242), Expect = 5.843e-21 Identity = 50/146 (34.25%), Postives = 78/146 (53.42%), Query Frame = 2
Query: 1085 DGRVLGTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNITKINIDWPDFD--LDLSAVEVINGLLLYDTSKRWGFAQLNSS---------DYIDYMRNCSDPPFIPNIAGNDDTGYHDFRNQIFEY 1489
D +V GTP+Y+ PE+++ Y G P+DWWA+G+I++E L G PF G+T EE+FG + I WP+ D L A ++I LL + +R G + D+ +R ++ FIP + DDT Y D R+ + +
Sbjct: 519 DKQVCGTPEYIAPEVILRQGY---GKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVVSDEIVWPEGDDALPADAQDLITRLLSQNPLERLGTGGASEVKQHPFFLGLDWNGLLRQKAE--FIPQLEAEDDTSYFDTRSDRYHH 659
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Medaka
Match: stk38l (serine/threonine kinase 38 like [Source:NCBI gene;Acc:101163624]) HSP 1 Score: 126.716 bits (317), Expect = 2.150e-31 Identity = 60/151 (39.74%), Postives = 98/151 (64.90%), Query Frame = 2
Query: 80 LSINNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGL 532
L +++F +K I +GA+G+V L +K D YA+K++ K +M K I AER+ L + ++V ++YS Q ++N+YL+ME+L GGD+ TL++ LS + Y E ++A++ +H IHRD+KPDN+L+ + GH+KL+DFGL
Sbjct: 85 LGLDDFESLKVIGRGAFGEVRL-VQKKDTGHIYAMKILRKADMLEKEQVAHIRAERDILVEADSAWVVKMFYSFQDKRNLYLIMEFLPGGDMMTLLMKKDTLSEEATQFYIAETVLAIDSIHQLGFIHRDIKPDNLLLDSRGHVKLSDFGL 234 HSP 2 Score: 59.3066 bits (142), Expect = 4.605e-9 Identity = 24/56 (42.86%), Postives = 35/56 (62.50%), Query Frame = 2
Query: 1094 VLGTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNI 1261
+GTPDY+ PE+ + + Y DWW+LGVI++EML G PF ET +E + +
Sbjct: 283 TVGTPDYIAPEVFMQTGY---NKLCDWWSLGVIMYEMLIGYPPFCSETPQETYRKV 335
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Medaka
Match: lats1 (large tumor suppressor kinase 1 [Source:NCBI gene;Acc:101157749]) HSP 1 Score: 129.798 bits (325), Expect = 2.182e-31 Identity = 64/146 (43.84%), Postives = 92/146 (63.01%), Query Frame = 2
Query: 95 FTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGL 532
F IK + GA+G+V L +K D YA+K + K+++ +N + AER+ LA + ++V LYYS Q + N+Y VMEY+ GGD+ +L++ G+ A Y E+ A+E +H IHRD+KPDNILI DGH+KLTDFGL
Sbjct: 651 FKQIKTLGIGAFGQVCL-ARKEDTGSLYAMKTLRKKDVLLRNQVAHVKAERDILAEADNEWVVRLYYSFQDKDNLYFVMEYIPGGDMMSLLIRLGIFKEDLAQFYIAELTCAVESVHKMGFIHRDIKPDNILIDRDGHIKLTDFGL 795 HSP 2 Score: 54.299 bits (129), Expect = 3.018e-7 Identity = 46/139 (33.09%), Postives = 68/139 (48.92%), Query Frame = 2
Query: 1094 VLGTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSG----ETVEEIFGNITKINIDWPDFDLDLSAVEVINGLLLYDTSKRWGFAQLNSSDYI---DYMRN---CSD-----PPFIPNIAGNDDTGYHD 1465
++GTP+Y+ PE+L+ S Y DWW++GVI++EM+ G PF ET +++ T ++I P L A ++I L D R G N +D I + R SD P+IP IA + DT D
Sbjct: 856 LVGTPNYIAPEVLLRSGYTQL---CDWWSVGVILYEMVVGQPPFFANTPLETQKKVINWKTTLHIP-PQAKLSPEASDLIV-KLCRDPKDRLG---TNGADEIKAHSFFRTIDFSSDLRQQVAPYIPTIAHSMDTSNFD 986
BLAST of Serine/threonine-protein kinase greatwall vs. Planmine SMEST
Match: SMESG000073737.1 (SMESG000073737.1) HSP 1 Score: 991.875 bits (2563), Expect = 0.000e+0 Identity = 477/480 (99.38%), Postives = 480/480 (100.00%), Query Frame = 2
Query: 74 MPLSINNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGLSKVTLTNTNLQINEILYTPSKSKSDINTEYLRTPNQLYSLMDKFELCNTMKTHVFSSKRIPQTEWKVKQSHRRKSDVLGDSWDTFISANNRNFYEDKQNSLASHVKNESEILSGIRNCSLDVKSPSKLKQENENEDVQFQNHFKTPERKHPMKASTPLRKKQLNLNDKLPSRSSDVLHYLDLTNDGRVLGTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNITKINIDWPDFDLDLSAVEVINGLLLYDTSKRWGFAQLNSSDYIDYMRNCSDPPFIPNIAGNDDTGYHDFRNQIFEYSSNLNIME 1513
MPLSINNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGLSKVTLTNTNLQINEILYTPSKSKSDINTEYLRTPNQLYSLMDKFELCNTMKTHVFSSKRIPQTEWKVKQSHRRKSDVLGDSWDTFISANNRNFYEDKQNSLASHVKNESEILSGIRNCSLDVKSP+KLKQENENEDVQFQNHFKTPERKHPMKASTPLRKKQLNLNDKLPSRSSDVLHYLDLTNDGRVLGTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNITKINIDWPDFDLDLSAVEVINGLLLYDTSKRWGFAQLNSSDYIDYMRNCSDPPFIP+IAGNDDTGYHDFRNQIF+YSSNLNIME
Sbjct: 1 MPLSINNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGLSKVTLTNTNLQINEILYTPSKSKSDINTEYLRTPNQLYSLMDKFELCNTMKTHVFSSKRIPQTEWKVKQSHRRKSDVLGDSWDTFISANNRNFYEDKQNSLASHVKNESEILSGIRNCSLDVKSPNKLKQENENEDVQFQNHFKTPERKHPMKASTPLRKKQLNLNDKLPSRSSDVLHYLDLTNDGRVLGTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNITKINIDWPDFDLDLSAVEVINGLLLYDTSKRWGFAQLNSSDYIDYMRNCSDPPFIPDIAGNDDTGYHDFRNQIFDYSSNLNIME 480
BLAST of Serine/threonine-protein kinase greatwall vs. Planmine SMEST
Match: SMESG000019748.1 (SMESG000019748.1) HSP 1 Score: 142.895 bits (359), Expect = 8.791e-36 Identity = 73/155 (47.10%), Postives = 104/155 (67.10%), Query Frame = 2
Query: 89 NNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGLSKVTLTN 553
++F IK I+ GAYG V+L +K + K +A+K + K + +N QI AER+ + + P++V LY + +T K + ++MEY+ GGD+ TLI G L + A +Y E ++ALEYLHN I+HRDLKPDN+LI +GH+KLTDFGLSKV L N
Sbjct: 873 SDFEDIKLISNGAYGAVHLVREKKTK-KRFAMKKIRKDNLLLRNQVEQIFAERDIMTFADSPFVVGLYCTFETRKYLCMIMEYVEGGDVATLIKSIGPLPDELARMYFAETVLALEYLHNFGIVHRDLKPDNLLITLEGHIKLTDFGLSKVGLMN 1026 HSP 2 Score: 92.4337 bits (228), Expect = 2.648e-19 Identity = 51/147 (34.69%), Postives = 76/147 (51.70%), Query Frame = 2
Query: 1085 DGRVLGTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNITKINIDW---PDFDLDLSAVEVINGLLLYDTSKRWGFAQLNSSDYIDYMRNC-------SDPPFIPNIAGNDDTGYHDFRNQIFEYSS 1495
D +V GTPDY+ PE+++ Y IDWWA+G+I++E + G TPF G++ EE+FGNI I+W PD + +AV +I LL D R G + ++ + FIP + DDT Y D R +++ S
Sbjct: 1045 DKQVFGTPDYIAPEVILRQGYDKM---IDWWAMGIILYEFIVGCTPFYGDSTEELFGNIISGEIEWPQDPDPCVPDNAVNLITELLKRDPLVRLGAEGTHEVKEHEFFSDVDWQGLLRQKALFIPVLDHEDDTSYFDPRTSRYQHES 1188
BLAST of Serine/threonine-protein kinase greatwall vs. Planmine SMEST
Match: SMESG000019748.1 (SMESG000019748.1) HSP 1 Score: 142.895 bits (359), Expect = 9.008e-36 Identity = 73/155 (47.10%), Postives = 104/155 (67.10%), Query Frame = 2
Query: 89 NNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGLSKVTLTN 553
++F IK I+ GAYG V+L +K + K +A+K + K + +N QI AER+ + + P++V LY + +T K + ++MEY+ GGD+ TLI G L + A +Y E ++ALEYLHN I+HRDLKPDN+LI +GH+KLTDFGLSKV L N
Sbjct: 674 SDFEDIKLISNGAYGAVHLVREKKTK-KRFAMKKIRKDNLLLRNQVEQIFAERDIMTFADSPFVVGLYCTFETRKYLCMIMEYVEGGDVATLIKSIGPLPDELARMYFAETVLALEYLHNFGIVHRDLKPDNLLITLEGHIKLTDFGLSKVGLMN 827 HSP 2 Score: 92.0485 bits (227), Expect = 3.524e-19 Identity = 53/149 (35.57%), Postives = 78/149 (52.35%), Query Frame = 2
Query: 1085 DGRVLGTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNITKINIDW---PDFDLDLSAVEVINGLLLYDTSKRWGFAQ---------LNSSDYIDYMRNCSDPPFIPNIAGNDDTGYHDFRNQIFEYSS 1495
D +V GTPDY+ PE+++ Y IDWWA+G+I++E + G TPF G++ EE+FGNI I+W PD + +AV +I LL D R G + D+ +R + FIP + DDT Y D R +++ S
Sbjct: 846 DKQVFGTPDYIAPEVILRQGYDKM---IDWWAMGIILYEFIVGCTPFYGDSTEELFGNIISGEIEWPQDPDPCVPDNAVNLITELLKRDPLVRLGAEGTHEVKEHEFFSDVDWQGLLRQKAL--FIPVLDHEDDTSYFDPRTSRYQHES 989
BLAST of Serine/threonine-protein kinase greatwall vs. Planmine SMEST
Match: SMESG000019748.1 (SMESG000019748.1) HSP 1 Score: 142.895 bits (359), Expect = 9.097e-36 Identity = 73/155 (47.10%), Postives = 104/155 (67.10%), Query Frame = 2
Query: 89 NNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGLSKVTLTN 553
++F IK I+ GAYG V+L +K + K +A+K + K + +N QI AER+ + + P++V LY + +T K + ++MEY+ GGD+ TLI G L + A +Y E ++ALEYLHN I+HRDLKPDN+LI +GH+KLTDFGLSKV L N
Sbjct: 677 SDFEDIKLISNGAYGAVHLVREKKTK-KRFAMKKIRKDNLLLRNQVEQIFAERDIMTFADSPFVVGLYCTFETRKYLCMIMEYVEGGDVATLIKSIGPLPDELARMYFAETVLALEYLHNFGIVHRDLKPDNLLITLEGHIKLTDFGLSKVGLMN 830 HSP 2 Score: 92.0485 bits (227), Expect = 3.404e-19 Identity = 53/149 (35.57%), Postives = 78/149 (52.35%), Query Frame = 2
Query: 1085 DGRVLGTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNITKINIDW---PDFDLDLSAVEVINGLLLYDTSKRWGFAQ---------LNSSDYIDYMRNCSDPPFIPNIAGNDDTGYHDFRNQIFEYSS 1495
D +V GTPDY+ PE+++ Y IDWWA+G+I++E + G TPF G++ EE+FGNI I+W PD + +AV +I LL D R G + D+ +R + FIP + DDT Y D R +++ S
Sbjct: 849 DKQVFGTPDYIAPEVILRQGYDKM---IDWWAMGIILYEFIVGCTPFYGDSTEELFGNIISGEIEWPQDPDPCVPDNAVNLITELLKRDPLVRLGAEGTHEVKEHEFFSDVDWQGLLRQKAL--FIPVLDHEDDTSYFDPRTSRYQHES 992
BLAST of Serine/threonine-protein kinase greatwall vs. Planmine SMEST
Match: SMESG000019748.1 (SMESG000019748.1) HSP 1 Score: 142.895 bits (359), Expect = 9.518e-36 Identity = 73/155 (47.10%), Postives = 104/155 (67.10%), Query Frame = 2
Query: 89 NNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLFHQINAERNALAISHCPYIVHLYYSLQTEKNVYLVMEYLCGGDLKTLILMCGMLSLQEASIYSREIMIALEYLHNHKIIHRDLKPDNILIANDGHLKLTDFGLSKVTLTN 553
++F IK I+ GAYG V+L +K + K +A+K + K + +N QI AER+ + + P++V LY + +T K + ++MEY+ GGD+ TLI G L + A +Y E ++ALEYLHN I+HRDLKPDN+LI +GH+KLTDFGLSKV L N
Sbjct: 635 SDFEDIKLISNGAYGAVHLVREKKTK-KRFAMKKIRKDNLLLRNQVEQIFAERDIMTFADSPFVVGLYCTFETRKYLCMIMEYVEGGDVATLIKSIGPLPDELARMYFAETVLALEYLHNFGIVHRDLKPDNLLITLEGHIKLTDFGLSKVGLMN 788 HSP 2 Score: 92.0485 bits (227), Expect = 3.231e-19 Identity = 53/149 (35.57%), Postives = 78/149 (52.35%), Query Frame = 2
Query: 1085 DGRVLGTPDYLPPEILINSKYRLQGPPIDWWALGVIIFEMLTGITPFSGETVEEIFGNITKINIDW---PDFDLDLSAVEVINGLLLYDTSKRWGFAQ---------LNSSDYIDYMRNCSDPPFIPNIAGNDDTGYHDFRNQIFEYSS 1495
D +V GTPDY+ PE+++ Y IDWWA+G+I++E + G TPF G++ EE+FGNI I+W PD + +AV +I LL D R G + D+ +R + FIP + DDT Y D R +++ S
Sbjct: 807 DKQVFGTPDYIAPEVILRQGYDKM---IDWWAMGIILYEFIVGCTPFYGDSTEELFGNIISGEIEWPQDPDPCVPDNAVNLITELLKRDPLVRLGAEGTHEVKEHEFFSDVDWQGLLRQKA--LFIPVLDHEDDTSYFDPRTSRYQHES 950 The following BLAST results are available for this feature:
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Serine/threonine-protein kinase greatwall vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Serine/threonine-protein kinase greatwall vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 5
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of Serine/threonine-protein kinase greatwall vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Serine/threonine-protein kinase greatwall vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30020331 ID=SMED30020331|Name=Serine/threonine-protein kinase greatwall|organism=Schmidtea mediterranea sexual|type=transcript|length=1540bpback to top protein sequence of SMED30020331-orf-1 >SMED30020331-orf-1 ID=SMED30020331-orf-1|Name=SMED30020331-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=481bp MPLSINNFTIIKQITKGAYGKVYLGTKKSDRTKYYAIKMVSKQEMKNKNLback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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