WD_REPEATS_REGION domain-containing protein

Overview
NameWD_REPEATS_REGION domain-containing protein
Smed IDSMED30020322
Length (bp)426
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of WD_REPEATS_REGION domain-containing protein (SMED30020322) t-SNE clustered cells

Violin plots show distribution of expression levels for WD_REPEATS_REGION domain-containing protein (SMED30020322) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of WD_REPEATS_REGION domain-containing protein (SMED30020322) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for WD_REPEATS_REGION domain-containing protein (SMED30020322) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30020322

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30020322SMESG000053685.1 SmedASXL_011596SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30020322SMESG000053685.1 SmedASXL_011596SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
neoblastSMED30020322SMESG000053685.1 dd_Smed_v4_5567_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of WD_REPEATS_REGION domain-containing protein vs. Ensembl Human
Match: WDR33 (WD repeat domain 33 [Source:HGNC Symbol;Acc:HGNC:25651])

HSP 1 Score: 52.373 bits (124), Expect = 3.589e-8
Identity = 21/33 (63.64%), Postives = 25/33 (75.76%), Query Frame = 2 
Query:  326 SSFCPSDKKFVTCSNDNTVRIWDFQTYKEERVL 424
            +SF P+D KF TCS+D TVRIWDF    EER+L
Sbjct:  208 ASFSPTDNKFATCSDDGTVRIWDFLRCHEERIL 240
[Close]
BLAST of WD_REPEATS_REGION domain-containing protein vs. Ensembl Human
Match: WDR33 (WD repeat domain 33 [Source:HGNC Symbol;Acc:HGNC:25651])

HSP 1 Score: 49.6766 bits (117), Expect = 1.678e-7
Identity = 21/33 (63.64%), Postives = 25/33 (75.76%), Query Frame = 2 
Query:  326 SSFCPSDKKFVTCSNDNTVRIWDFQTYKEERVL 424
            +SF P+D KF TCS+D TVRIWDF    EER+L
Sbjct:  130 ASFSPTDNKFATCSDDGTVRIWDFLRCHEERIL 162
[Close]
BLAST of WD_REPEATS_REGION domain-containing protein vs. Ensembl Human
Match: WDR33 (WD repeat domain 33 [Source:HGNC Symbol;Acc:HGNC:25651])

HSP 1 Score: 49.2914 bits (116), Expect = 2.965e-7
Identity = 21/33 (63.64%), Postives = 25/33 (75.76%), Query Frame = 2 
Query:  326 SSFCPSDKKFVTCSNDNTVRIWDFQTYKEERVL 424
            +SF P+D KF TCS+D TVRIWDF    EER+L
Sbjct:  208 ASFSPTDNKFATCSDDGTVRIWDFLRCHEERIL 240
[Close]
BLAST of WD_REPEATS_REGION domain-containing protein vs. Ensembl Celegans
Match: pfs-2 (Polyadenylation Factor Subunit homolog [Source:UniProtKB/TrEMBL;Acc:U4PN22])

HSP 1 Score: 48.9062 bits (115), Expect = 2.036e-7
Identity = 19/32 (59.38%), Postives = 23/32 (71.88%), Query Frame = 2 
Query:  329 SFCPSDKKFVTCSNDNTVRIWDFQTYKEERVL 424
            +F P+D KF T S+D T R+WDF  Y EERVL
Sbjct:   19 AFAPTDVKFATASDDGTARVWDFARYTEERVL 50
[Close]
BLAST of WD_REPEATS_REGION domain-containing protein vs. Ensembl Celegans
Match: pfs-2 (Polyadenylation Factor Subunit homolog [Source:UniProtKB/TrEMBL;Acc:U4PN22])

HSP 1 Score: 48.9062 bits (115), Expect = 2.115e-7
Identity = 19/32 (59.38%), Postives = 23/32 (71.88%), Query Frame = 2 
Query:  329 SFCPSDKKFVTCSNDNTVRIWDFQTYKEERVL 424
            +F P+D KF T S+D T R+WDF  Y EERVL
Sbjct:  235 AFAPTDVKFATASDDGTARVWDFARYTEERVL 266
[Close]
BLAST of WD_REPEATS_REGION domain-containing protein vs. Ensembl Fly
Match: Wdr33 (gene:FBgn0046222 transcript:FBtr0078713)

HSP 1 Score: 48.521 bits (114), Expect = 3.973e-7
Identity = 20/32 (62.50%), Postives = 25/32 (78.12%), Query Frame = 2 
Query:  329 SFCPSDKKFVTCSNDNTVRIWDFQTYKEERVL 424
            SF P+D KFV+ S+D T+RIWDF   +EERVL
Sbjct:  236 SFSPTDSKFVSGSDDGTLRIWDFMRCQEERVL 267
[Close]
BLAST of WD_REPEATS_REGION domain-containing protein vs. Ensembl Fly
Match: Wdr33 (gene:FBgn0046222 transcript:FBtr0078714)

HSP 1 Score: 48.521 bits (114), Expect = 3.973e-7
Identity = 20/32 (62.50%), Postives = 25/32 (78.12%), Query Frame = 2 
Query:  329 SFCPSDKKFVTCSNDNTVRIWDFQTYKEERVL 424
            SF P+D KFV+ S+D T+RIWDF   +EERVL
Sbjct:  236 SFSPTDSKFVSGSDDGTLRIWDFMRCQEERVL 267
[Close]
BLAST of WD_REPEATS_REGION domain-containing protein vs. Ensembl Zebrafish
Match: wdr33 (WD repeat domain 33 [Source:ZFIN;Acc:ZDB-GENE-040924-3])

HSP 1 Score: 52.373 bits (124), Expect = 2.253e-8
Identity = 21/33 (63.64%), Postives = 25/33 (75.76%), Query Frame = 2 
Query:  326 SSFCPSDKKFVTCSNDNTVRIWDFQTYKEERVL 424
            +SF P+D KF TCS+D TVRIWDF    EER+L
Sbjct:  208 ASFSPTDNKFATCSDDGTVRIWDFLRCHEERIL 240
[Close]
BLAST of WD_REPEATS_REGION domain-containing protein vs. Ensembl Zebrafish
Match: wdr33 (WD repeat domain 33 [Source:ZFIN;Acc:ZDB-GENE-040924-3])

HSP 1 Score: 52.373 bits (124), Expect = 2.256e-8
Identity = 21/33 (63.64%), Postives = 25/33 (75.76%), Query Frame = 2 
Query:  326 SSFCPSDKKFVTCSNDNTVRIWDFQTYKEERVL 424
            +SF P+D KF TCS+D TVRIWDF    EER+L
Sbjct:  208 ASFSPTDNKFATCSDDGTVRIWDFLRCHEERIL 240
[Close]
BLAST of WD_REPEATS_REGION domain-containing protein vs. Ensembl Xenopus
Match: ntf4 (neurotrophin 4 [Source:Xenbase;Acc:XB-GENE-1013492])

HSP 1 Score: 52.373 bits (124), Expect = 3.193e-8
Identity = 21/33 (63.64%), Postives = 25/33 (75.76%), Query Frame = 2 
Query:  326 SSFCPSDKKFVTCSNDNTVRIWDFQTYKEERVL 424
            +SF P+D KF TCS+D TVRIWDF    EER+L
Sbjct:  208 ASFSPTDNKFATCSDDGTVRIWDFLRCHEERIL 240
[Close]
BLAST of WD_REPEATS_REGION domain-containing protein vs. Ensembl Xenopus
Match: ntf4 (neurotrophin 4 [Source:Xenbase;Acc:XB-GENE-1013492])

HSP 1 Score: 52.373 bits (124), Expect = 3.196e-8
Identity = 21/33 (63.64%), Postives = 25/33 (75.76%), Query Frame = 2 
Query:  326 SSFCPSDKKFVTCSNDNTVRIWDFQTYKEERVL 424
            +SF P+D KF TCS+D TVRIWDF    EER+L
Sbjct:  208 ASFSPTDNKFATCSDDGTVRIWDFLRCHEERIL 240
[Close]
BLAST of WD_REPEATS_REGION domain-containing protein vs. Ensembl Xenopus
Match: ntf4 (neurotrophin 4 [Source:Xenbase;Acc:XB-GENE-1013492])

HSP 1 Score: 52.373 bits (124), Expect = 3.241e-8
Identity = 21/33 (63.64%), Postives = 25/33 (75.76%), Query Frame = 2 
Query:  326 SSFCPSDKKFVTCSNDNTVRIWDFQTYKEERVL 424
            +SF P+D KF TCS+D TVRIWDF    EER+L
Sbjct:  208 ASFSPTDNKFATCSDDGTVRIWDFLRCHEERIL 240
[Close]
BLAST of WD_REPEATS_REGION domain-containing protein vs. Ensembl Xenopus
Match: ntf4 (neurotrophin 4 [Source:Xenbase;Acc:XB-GENE-1013492])

HSP 1 Score: 52.373 bits (124), Expect = 3.287e-8
Identity = 21/33 (63.64%), Postives = 25/33 (75.76%), Query Frame = 2 
Query:  326 SSFCPSDKKFVTCSNDNTVRIWDFQTYKEERVL 424
            +SF P+D KF TCS+D TVRIWDF    EER+L
Sbjct:  208 ASFSPTDNKFATCSDDGTVRIWDFLRCHEERIL 240
[Close]
BLAST of WD_REPEATS_REGION domain-containing protein vs. Ensembl Xenopus
Match: ntf4 (neurotrophin 4 [Source:Xenbase;Acc:XB-GENE-1013492])

HSP 1 Score: 49.6766 bits (117), Expect = 2.247e-7
Identity = 21/33 (63.64%), Postives = 25/33 (75.76%), Query Frame = 2 
Query:  326 SSFCPSDKKFVTCSNDNTVRIWDFQTYKEERVL 424
            +SF P+D KF TCS+D TVRIWDF    EER+L
Sbjct:  208 ASFSPTDNKFATCSDDGTVRIWDFLRCHEERIL 240
[Close]
BLAST of WD_REPEATS_REGION domain-containing protein vs. Ensembl Mouse
Match: Wdr33 (WD repeat domain 33 [Source:MGI Symbol;Acc:MGI:1921570])

HSP 1 Score: 52.373 bits (124), Expect = 2.463e-8
Identity = 21/33 (63.64%), Postives = 25/33 (75.76%), Query Frame = 2 
Query:  326 SSFCPSDKKFVTCSNDNTVRIWDFQTYKEERVL 424
            +SF P+D KF TCS+D TVRIWDF    EER+L
Sbjct:  208 ASFSPTDNKFATCSDDGTVRIWDFLRCHEERIL 240
[Close]
BLAST of WD_REPEATS_REGION domain-containing protein vs. Ensembl Mouse
Match: Wdr33 (WD repeat domain 33 [Source:MGI Symbol;Acc:MGI:1921570])

HSP 1 Score: 49.6766 bits (117), Expect = 1.364e-7
Identity = 21/33 (63.64%), Postives = 25/33 (75.76%), Query Frame = 2 
Query:  326 SSFCPSDKKFVTCSNDNTVRIWDFQTYKEERVL 424
            +SF P+D KF TCS+D TVRIWDF    EER+L
Sbjct:  208 ASFSPTDNKFATCSDDGTVRIWDFLRCHEERIL 240
[Close]
BLAST of WD_REPEATS_REGION domain-containing protein vs. Ensembl Mouse
Match: Wdr33 (WD repeat domain 33 [Source:MGI Symbol;Acc:MGI:1921570])

HSP 1 Score: 49.2914 bits (116), Expect = 2.095e-7
Identity = 21/33 (63.64%), Postives = 25/33 (75.76%), Query Frame = 2 
Query:  326 SSFCPSDKKFVTCSNDNTVRIWDFQTYKEERVL 424
            +SF P+D KF TCS+D TVRIWDF    EER+L
Sbjct:  208 ASFSPTDNKFATCSDDGTVRIWDFLRCHEERIL 240
[Close]
BLAST of WD_REPEATS_REGION domain-containing protein vs. UniProt/SwissProt
Match: sp|Q8K4P0|WDR33_MOUSE (pre-mRNA 3' end processing protein WDR33 OS=Mus musculus OX=10090 GN=Wdr33 PE=1 SV=1)

HSP 1 Score: 52.373 bits (124), Expect = 1.723e-7
Identity = 21/33 (63.64%), Postives = 25/33 (75.76%), Query Frame = 2 
Query:  326 SSFCPSDKKFVTCSNDNTVRIWDFQTYKEERVL 424
            +SF P+D KF TCS+D TVRIWDF    EER+L
Sbjct:  208 ASFSPTDNKFATCSDDGTVRIWDFLRCHEERIL 240
[Close]
BLAST of WD_REPEATS_REGION domain-containing protein vs. UniProt/SwissProt
Match: sp|Q9C0J8|WDR33_HUMAN (pre-mRNA 3' end processing protein WDR33 OS=Homo sapiens OX=9606 GN=WDR33 PE=1 SV=2)

HSP 1 Score: 52.373 bits (124), Expect = 1.724e-7
Identity = 21/33 (63.64%), Postives = 25/33 (75.76%), Query Frame = 2 
Query:  326 SSFCPSDKKFVTCSNDNTVRIWDFQTYKEERVL 424
            +SF P+D KF TCS+D TVRIWDF    EER+L
Sbjct:  208 ASFSPTDNKFATCSDDGTVRIWDFLRCHEERIL 240
[Close]
BLAST of WD_REPEATS_REGION domain-containing protein vs. UniProt/SwissProt
Match: sp|U4PCM1|WDR33_CAEEL (pre-mRNA 3' end processing protein pfs-2 OS=Caenorhabditis elegans OX=6239 GN=pfs-2 PE=1 SV=1)

HSP 1 Score: 48.9062 bits (115), Expect = 2.691e-6
Identity = 19/32 (59.38%), Postives = 23/32 (71.88%), Query Frame = 2 
Query:  329 SFCPSDKKFVTCSNDNTVRIWDFQTYKEERVL 424
            +F P+D KF T S+D T R+WDF  Y EERVL
Sbjct:  235 AFAPTDVKFATASDDGTARVWDFARYTEERVL 266
[Close]
BLAST of WD_REPEATS_REGION domain-containing protein vs. UniProt/SwissProt
Match: sp|Q75AV4|PFS2_ASHGO (Polyadenylation factor subunit 2 OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) OX=284811 GN=PSF2 PE=3 SV=1)

HSP 1 Score: 47.7506 bits (112), Expect = 7.071e-6
Identity = 19/32 (59.38%), Postives = 26/32 (81.25%), Query Frame = 2 
Query:  329 SFCPSDKKFVTCSNDNTVRIWDFQTYKEERVL 424
            SF  SD+KFVTCS+DN ++IW+F   ++ERVL
Sbjct:  170 SFSYSDQKFVTCSDDNVLKIWNFSNGQQERVL 201
[Close]
BLAST of WD_REPEATS_REGION domain-containing protein vs. UniProt/SwissProt
Match: sp|Q6CP71|PFS2_KLULA (Polyadenylation factor subunit 2 OS=Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) OX=284590 GN=PFS2 PE=3 SV=1)

HSP 1 Score: 47.7506 bits (112), Expect = 7.574e-6
Identity = 18/32 (56.25%), Postives = 27/32 (84.38%), Query Frame = 2 
Query:  329 SFCPSDKKFVTCSNDNTVRIWDFQTYKEERVL 424
            SF P+D+KFV+CS+DN ++IW+F   ++ERVL
Sbjct:  172 SFSPTDQKFVSCSDDNVLKIWNFSNGQQERVL 203
[Close]
BLAST of WD_REPEATS_REGION domain-containing protein vs. TrEMBL
Match: A0A267GFK9 (WD_REPEATS_REGION domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig012484g1 PE=4 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 3.374e-7
Identity = 24/32 (75.00%), Postives = 27/32 (84.38%), Query Frame = 2 
Query:  329 SFCPSDKKFVTCSNDNTVRIWDFQTYKEERVL 424
            SFCP+D KFVTCS+D TVRIWDF   +EERVL
Sbjct:  187 SFCPTDTKFVTCSDDATVRIWDFVRCREERVL 218
[Close]
BLAST of WD_REPEATS_REGION domain-containing protein vs. TrEMBL
Match: A0A267F6P0 (WD_REPEATS_REGION domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig018914g1 PE=4 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 3.374e-7
Identity = 24/32 (75.00%), Postives = 27/32 (84.38%), Query Frame = 2 
Query:  329 SFCPSDKKFVTCSNDNTVRIWDFQTYKEERVL 424
            SFCP+D KFVTCS+D TVRIWDF   +EERVL
Sbjct:  187 SFCPTDTKFVTCSDDATVRIWDFVRCREERVL 218
[Close]
BLAST of WD_REPEATS_REGION domain-containing protein vs. TrEMBL
Match: A0A267DH23 (WD_REPEATS_REGION domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig000955g1 PE=4 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 3.396e-7
Identity = 24/32 (75.00%), Postives = 27/32 (84.38%), Query Frame = 2 
Query:  329 SFCPSDKKFVTCSNDNTVRIWDFQTYKEERVL 424
            SFCP+D KFVTCS+D TVRIWDF   +EERVL
Sbjct:  187 SFCPTDTKFVTCSDDATVRIWDFVRCREERVL 218
[Close]
BLAST of WD_REPEATS_REGION domain-containing protein vs. TrEMBL
Match: A0A419PEB0 (Pre-mRNA 3' end processing protein WDR33 (Fragment) OS=Clonorchis sinensis OX=79923 GN=WDR33 PE=4 SV=1)

HSP 1 Score: 58.151 bits (139), Expect = 4.955e-7
Identity = 24/32 (75.00%), Postives = 26/32 (81.25%), Query Frame = 2 
Query:  329 SFCPSDKKFVTCSNDNTVRIWDFQTYKEERVL 424
            SFCP D KFVTCS+D+TVRIWDF    EERVL
Sbjct:   43 SFCPFDNKFVTCSDDSTVRIWDFHRCAEERVL 74
[Close]
BLAST of WD_REPEATS_REGION domain-containing protein vs. TrEMBL
Match: U4U5R8 (WD_REPEATS_REGION domain-containing protein OS=Dendroctonus ponderosae OX=77166 GN=D910_02726 PE=4 SV=1)

HSP 1 Score: 58.151 bits (139), Expect = 5.052e-7
Identity = 24/32 (75.00%), Postives = 27/32 (84.38%), Query Frame = 2 
Query:  329 SFCPSDKKFVTCSNDNTVRIWDFQTYKEERVL 424
            SF PSD KFVTCS+D T+RIWDF  Y+EERVL
Sbjct:  227 SFSPSDNKFVTCSDDGTLRIWDFFRYQEERVL 258
[Close]
BLAST of WD_REPEATS_REGION domain-containing protein vs. Ensembl Cavefish
Match: WDR33 (WD repeat domain 33 [Source:HGNC Symbol;Acc:HGNC:25651])

HSP 1 Score: 52.373 bits (124), Expect = 1.898e-8
Identity = 21/33 (63.64%), Postives = 25/33 (75.76%), Query Frame = 2 
Query:  326 SSFCPSDKKFVTCSNDNTVRIWDFQTYKEERVL 424
            +SF P+D KF TCS+D TVRIWDF    EER+L
Sbjct:  117 ASFSPTDNKFATCSDDGTVRIWDFLRCHEERIL 149
[Close]
BLAST of WD_REPEATS_REGION domain-containing protein vs. Ensembl Cavefish
Match: WDR33 (WD repeat domain 33 [Source:HGNC Symbol;Acc:HGNC:25651])

HSP 1 Score: 52.373 bits (124), Expect = 1.905e-8
Identity = 21/33 (63.64%), Postives = 25/33 (75.76%), Query Frame = 2 
Query:  326 SSFCPSDKKFVTCSNDNTVRIWDFQTYKEERVL 424
            +SF P+D KF TCS+D TVRIWDF    EER+L
Sbjct:  117 ASFSPTDNKFATCSDDGTVRIWDFLRCHEERIL 149
[Close]
BLAST of WD_REPEATS_REGION domain-containing protein vs. Ensembl Sea Lamprey
Match: WDR33 (WD repeat domain 33 [Source:HGNC Symbol;Acc:HGNC:25651])

HSP 1 Score: 52.7582 bits (125), Expect = 3.047e-9
Identity = 20/33 (60.61%), Postives = 25/33 (75.76%), Query Frame = 2 
Query:  326 SSFCPSDKKFVTCSNDNTVRIWDFQTYKEERVL 424
            +SF P+D KF TCS+D TVR+WDF    EER+L
Sbjct:  208 ASFAPTDNKFATCSDDGTVRVWDFMRCHEERIL 240
[Close]
BLAST of WD_REPEATS_REGION domain-containing protein vs. Ensembl Sea Lamprey
Match: WDR33 (WD repeat domain 33 [Source:HGNC Symbol;Acc:HGNC:25651])

HSP 1 Score: 52.7582 bits (125), Expect = 3.052e-9
Identity = 20/33 (60.61%), Postives = 25/33 (75.76%), Query Frame = 2 
Query:  326 SSFCPSDKKFVTCSNDNTVRIWDFQTYKEERVL 424
            +SF P+D KF TCS+D TVR+WDF    EER+L
Sbjct:  208 ASFAPTDNKFATCSDDGTVRVWDFMRCHEERIL 240
[Close]
BLAST of WD_REPEATS_REGION domain-containing protein vs. Ensembl Yeast
Match: PFS2 (Integral subunit of the pre-mRNA CPF complex; the cleavage and polyadenylation factor (CPF) complex plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex [Source:SGD;Acc:S000005261])

HSP 1 Score: 46.2098 bits (108), Expect = 3.820e-7
Identity = 18/32 (56.25%), Postives = 25/32 (78.12%), Query Frame = 2 
Query:  329 SFCPSDKKFVTCSNDNTVRIWDFQTYKEERVL 424
            +F  +D KFVTCS+DN ++IW+F   K+ERVL
Sbjct:  184 AFSSNDSKFVTCSDDNILKIWNFSNGKQERVL 215
[Close]
BLAST of WD_REPEATS_REGION domain-containing protein vs. Ensembl Medaka
Match: WDR33 (WD repeat domain 33 [Source:NCBI gene;Acc:101171438])

HSP 1 Score: 52.373 bits (124), Expect = 1.891e-8
Identity = 21/33 (63.64%), Postives = 25/33 (75.76%), Query Frame = 2 
Query:  326 SSFCPSDKKFVTCSNDNTVRIWDFQTYKEERVL 424
            +SF P+D KF TCS+D TVRIWDF    EER+L
Sbjct:  208 ASFSPTDNKFATCSDDGTVRIWDFLRCHEERIL 240
[Close]
BLAST of WD_REPEATS_REGION domain-containing protein vs. Ensembl Medaka
Match: WDR33 (WD repeat domain 33 [Source:NCBI gene;Acc:101171438])

HSP 1 Score: 52.373 bits (124), Expect = 1.891e-8
Identity = 21/33 (63.64%), Postives = 25/33 (75.76%), Query Frame = 2 
Query:  326 SSFCPSDKKFVTCSNDNTVRIWDFQTYKEERVL 424
            +SF P+D KF TCS+D TVRIWDF    EER+L
Sbjct:  208 ASFSPTDNKFATCSDDGTVRIWDFLRCHEERIL 240
[Close]
BLAST of WD_REPEATS_REGION domain-containing protein vs. Planmine SMEST
Match: SMESG000053685.1 (SMESG000053685.1)

HSP 1 Score: 73.1738 bits (178), Expect = 9.318e-16
Identity = 32/32 (100.00%), Postives = 32/32 (100.00%), Query Frame = 2 
Query:  329 SFCPSDKKFVTCSNDNTVRIWDFQTYKEERVL 424
            SFCPSDKKFVTCSNDNTVRIWDFQTYKEERVL
Sbjct:  212 SFCPSDKKFVTCSNDNTVRIWDFQTYKEERVL 243
The following BLAST results are available for this feature:
BLAST of WD_REPEATS_REGION domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 3
Match NameE-valueIdentityDescription
WDR333.589e-863.64WD repeat domain 33 [Source:HGNC Symbol;Acc:HGNC:2... [more]
WDR331.678e-763.64WD repeat domain 33 [Source:HGNC Symbol;Acc:HGNC:2... [more]
WDR332.965e-763.64WD repeat domain 33 [Source:HGNC Symbol;Acc:HGNC:2... [more]
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BLAST of WD_REPEATS_REGION domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2
Match NameE-valueIdentityDescription
pfs-22.036e-759.38Polyadenylation Factor Subunit homolog [Source:Un... [more]
pfs-22.115e-759.38Polyadenylation Factor Subunit homolog [Source:Un... [more]
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BLAST of WD_REPEATS_REGION domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Wdr333.973e-762.50gene:FBgn0046222 transcript:FBtr0078713[more]
Wdr333.973e-762.50gene:FBgn0046222 transcript:FBtr0078714[more]
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BLAST of WD_REPEATS_REGION domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
wdr332.253e-863.64WD repeat domain 33 [Source:ZFIN;Acc:ZDB-GENE-0409... [more]
wdr332.256e-863.64WD repeat domain 33 [Source:ZFIN;Acc:ZDB-GENE-0409... [more]
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BLAST of WD_REPEATS_REGION domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ntf43.193e-863.64neurotrophin 4 [Source:Xenbase;Acc:XB-GENE-1013492... [more]
ntf43.196e-863.64neurotrophin 4 [Source:Xenbase;Acc:XB-GENE-1013492... [more]
ntf43.241e-863.64neurotrophin 4 [Source:Xenbase;Acc:XB-GENE-1013492... [more]
ntf43.287e-863.64neurotrophin 4 [Source:Xenbase;Acc:XB-GENE-1013492... [more]
ntf42.247e-763.64neurotrophin 4 [Source:Xenbase;Acc:XB-GENE-1013492... [more]
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BLAST of WD_REPEATS_REGION domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 3
Match NameE-valueIdentityDescription
Wdr332.463e-863.64WD repeat domain 33 [Source:MGI Symbol;Acc:MGI:192... [more]
Wdr331.364e-763.64WD repeat domain 33 [Source:MGI Symbol;Acc:MGI:192... [more]
Wdr332.095e-763.64WD repeat domain 33 [Source:MGI Symbol;Acc:MGI:192... [more]
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BLAST of WD_REPEATS_REGION domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q8K4P0|WDR33_MOUSE1.723e-763.64pre-mRNA 3' end processing protein WDR33 OS=Mus mu... [more]
sp|Q9C0J8|WDR33_HUMAN1.724e-763.64pre-mRNA 3' end processing protein WDR33 OS=Homo s... [more]
sp|U4PCM1|WDR33_CAEEL2.691e-659.38pre-mRNA 3' end processing protein pfs-2 OS=Caenor... [more]
sp|Q75AV4|PFS2_ASHGO7.071e-659.38Polyadenylation factor subunit 2 OS=Ashbya gossypi... [more]
sp|Q6CP71|PFS2_KLULA7.574e-656.25Polyadenylation factor subunit 2 OS=Kluyveromyces ... [more]
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BLAST of WD_REPEATS_REGION domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A267GFK93.374e-775.00WD_REPEATS_REGION domain-containing protein OS=Mac... [more]
A0A267F6P03.374e-775.00WD_REPEATS_REGION domain-containing protein OS=Mac... [more]
A0A267DH233.396e-775.00WD_REPEATS_REGION domain-containing protein OS=Mac... [more]
A0A419PEB04.955e-775.00Pre-mRNA 3' end processing protein WDR33 (Fragment... [more]
U4U5R85.052e-775.00WD_REPEATS_REGION domain-containing protein OS=Den... [more]
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BLAST of WD_REPEATS_REGION domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
WDR331.898e-863.64WD repeat domain 33 [Source:HGNC Symbol;Acc:HGNC:2... [more]
WDR331.905e-863.64WD repeat domain 33 [Source:HGNC Symbol;Acc:HGNC:2... [more]
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BLAST of WD_REPEATS_REGION domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 2
Match NameE-valueIdentityDescription
WDR333.047e-960.61WD repeat domain 33 [Source:HGNC Symbol;Acc:HGNC:2... [more]
WDR333.052e-960.61WD repeat domain 33 [Source:HGNC Symbol;Acc:HGNC:2... [more]
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BLAST of WD_REPEATS_REGION domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
PFS23.820e-756.25Integral subunit of the pre-mRNA CPF complex; the ... [more]
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BLAST of WD_REPEATS_REGION domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of WD_REPEATS_REGION domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
WDR331.891e-863.64WD repeat domain 33 [Source:NCBI gene;Acc:10117143... [more]
WDR331.891e-863.64WD repeat domain 33 [Source:NCBI gene;Acc:10117143... [more]
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BLAST of WD_REPEATS_REGION domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000053685.19.318e-16100.00SMESG000053685.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30020322 ID=SMED30020322|Name=WD_REPEATS_REGION domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=426bp
AAAGACTCTGTCCGTGGTGTAAGGTACTAAAAATAATTCATGACAACTCT
TTGTAAAAACATATCTTTAATTACTATTTTTAATATTTTGTAAATAAAAT
TCGATTTTCTTTGATTTTTCTATGATTTTTTATCAGAAAGAGAGAGAGAA
AGAAAGAGAGATAGCTTTGCAAATATATTCGGTGTTCTTTCTGTTTTCAA
TCATCATAATAATAATTATATCAATAATTGTGCTAATCAATAACTTCATT
AGGTTTCATGGTTTGAAAAATTCAAATTCAAATTTGATGCTTTGTGTTGT
TTGTTCATTAATTTTTTTTAATCGCTCTAGTTTTTGTCCGTCGGATAAGA
AATTTGTTACATGTAGTAATGACAATACCGTCAGAATATGGGACTTTCAG
ACCTACAAAGAAGAACGGGTTTTAAG
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protein sequence of SMED30020322-orf-1

>SMED30020322-orf-1 ID=SMED30020322-orf-1|Name=SMED30020322-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=101bp
MIFYQKEREKEREIALQIYSVFFLFSIIIIIIISIIVLINNFIRFHGLKN
SNSNLMLCVVCSLIFFNRSSFCPSDKKFVTCSNDNTVRIWDFQTYKEERV
L
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002109X1 cell
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR036322WD40_repeat_dom_sf
IPR015943WD40/YVTN_repeat-like_dom_sf
IPR001680WD40_repeat
IPR017986WD40_repeat_dom
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
GO:0003723RNA binding
Vocabulary: cellular component
TermDefinition
GO:0001650fibrillar center
GO:0005581collagen trimer
GO:0005634nucleus
GO:0005654nucleoplasm
GO:0005847mRNA cleavage and polyadenylation specificity factor complex
Vocabulary: biological process
TermDefinition
GO:0006379mRNA cleavage
GO:0006397mRNA processing
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015943WD40/YVTN repeat-like-containing domain superfamilyGENE3DG3DSA:2.130.10.10coord: 44..101
e-value: 1.7E-7
score: 32.7
IPR017986WD40-repeat-containing domainPROSITEPS50294WD_REPEATS_REGIONcoord: 70..101
score: 9.283
IPR001680WD40 repeatPROSITEPS50082WD_REPEATS_2coord: 70..100
score: 10.742
IPR036322WD40-repeat-containing domain superfamilySUPERFAMILYSSF50978WD40 repeat-likecoord: 70..96
NoneNo IPR availableTMHMMTMhelixcoord: 53..72
NoneNo IPR availableTMHMMTMhelixcoord: 21..43