Kinase D-interacting substrate
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30020234 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 11
Homology
BLAST of Kinase D-interacting substrate vs. Ensembl Human
Match: KIDINS220 (kinase D interacting substrate 220 [Source:HGNC Symbol;Acc:HGNC:29508]) HSP 1 Score: 173.326 bits (438), Expect = 5.318e-43 Identity = 193/849 (22.73%), Postives = 364/849 (42.87%), Query Frame = 2
Query: 428 YIQALADMLVCPNTNFPISIGLFSRVGAGIRTTHLEKLSDELISLTKINNQKTLQPSLLLFMFPXXXXXXXXXXXXXXTNWIVGVSLGASLSLMSFLLIEIIIFGSNHK--RWGKALRAARWLASFTQSLQLFLDILFRYPHE-NELTTK--PLSIIITHHNLSNYSSASHMTLSIVEKLWKAVEKKYSCLLSRL--QISCKNPESSTKFKNICCIPIFVLVIIFFSAIIINATLFTIYNKNPMDIFVNKGLLPLFVIMTCIVFLCIFGTLPLVCRT----ITFLVRSPSRQLKQFMES-ENIDFQASDTLVKNELLKLCQIISIFEAYLNSEKIRMFVFIDAADTESREKLANSIYLVNGLLQMSSNAPIALILAADFQTLNTEWXXXXXXXXXXXXXXXXIFQEISDNFH-LIHSTIHLPIYMEEGIDFNRNSSGKKKY----ASNGMFCIRGGNSFE-------SLNMVTTGTKQNSVPVSTRKTSIYXXXXXXXXXXXXXKGASKSIQQLFLTRGDLSDINNKMIKQLTAAAAFIGRLLRINLIEFKLENLISWLSISINWPYQTALMALFLEEQCANLTPKHTLLQIHEQVLSKIGSKDSNPAEIELLARDDRDFGKLDAFIKKDVGATLTFSILKYFVSWSPFMNPYLRYVLRDYLTNKNLAQTRGSRNQLT-------------SPPLTSMALSDPSDVPMDEDLPQP----IISPSTGSGLVNQITSWSKSSIGNLAHKGDRPKT-PIAIKRCMVKLKDGYPGKDLSQLDKYEIGILMCQIEGLSSEENIEKYKEKLIQLNVTGLVLSVCDMDQLQNEMEMNFGDWQLFKTLIYHLRSVEA 2848
Y ALAD+L P PI +GL+++ G+G ++ L+KL DE+ + + Q S L+ + L +G++ + +G+++ S + ++ +I FG + W A + LA L+L L ++F P E E TTK P+ + T +N + ++ L A E+++ L +RL ++ + K+K CC+P FV+ + II TL I+ +P + VN + + + +V L L CRT + L+ S ++L + + ++K E+ + ++ +++ ++ R+ V ID D ++K+ + V L S P I A+D + + + +++ ++ + N H + + +HLP+++ +R S +K+ A+NG + S N + TK S R+ + + ++ S + +L +T SDI+ + +++L + GRLLR N I F + L SW++++ WPY+T+ + L+LEE + + TL I+E++ I + ++E L D D + F+ L +K F+ + ++P LR ++ D R +R Q++ PP S P V P ++SP S + +T ++G P P+ + L+ L+ + + QIEGL + + +Y + + N+ G VL+ C++D+L+ EM MNFGDW LF++ + +R+ E+
Sbjct: 190 YSSALADILSEPTMQPPICVGLYAQWGSG-KSFLLKKLEDEMKTFAGQQIEPLFQFSWLIVFLTLLLCGGLGLLFAFTVHPNLGIAVSLSFLALLYIFFIVIYFGGRREGESWNWAWVLSTRLARHIGYLELLLKLMFVNPPELPEQTTKALPVRFLFTDYNRLSSVGGETSLAEMIATLSDACEREFGFLATRLFRVFKTEDTQGKKKWKKTCCLPSFVIFLFIIGCIISGITLLAIFRVDPKHLTVNA----VLISIASVVGLAFV----LNCRTWWQVLDSLLNSQRKRLHNAASKLHKLKSEGFMKVLKCEVELMARMAKTIDSFTQNQ-TRLVVIIDGLDACEQDKVLQMLDTVRVLF---SKGPFIAIFASDPHII----------IKAINQNLNSVLRDSNINGHDYMRNIVHLPVFLN-----SRGLSNARKFLVTSATNGDVPCSDTTGIQEDADRRVSQNSLGEMTKLGSKTALNRRDT----YRRRQMQRTITRQMSFDLTKLLVTEDWFSDISPQTMRRLLNIVSVTGRLLRANQISFNWDRLASWINLTEQWPYRTSWLILYLEE-TEGIPDQMTLKTIYERISKNIPTT----KDVEPLLEIDGDIRNFEVFLSSRT-PVLVARDVKVFLPCTVNLDPKLREIIAD---------VRAAREQISIGGLAYPPLPLHEGPPRAPSGYSQPPSVCSSTSFNGPFAGGVVSPQPHSSYYSGMTGPQHP----FYNRGSGPAPGPVVL---------------LNSLNVDAVCEKLKQIEGL-DQSMLPQYCTTIKKANINGRVLAQCNIDELKKEMNMNFGDWHLFRSTVLEMRNAES 971
BLAST of Kinase D-interacting substrate vs. Ensembl Human
Match: KIDINS220 (kinase D interacting substrate 220 [Source:HGNC Symbol;Acc:HGNC:29508]) HSP 1 Score: 173.711 bits (439), Expect = 7.451e-43 Identity = 194/868 (22.35%), Postives = 369/868 (42.51%), Query Frame = 2
Query: 428 YIQALADMLVCPNTNFPISIGLFSRVGAGIRTTHLEKLSDELISLTKINNQKTLQPSLLLFMFPXXXXXXXXXXXXXXTNWIVGVSLGASLSLMSFLLIEIIIFGSNHK--RWGKALRAARWLASFTQSLQLFLDILFRYPHE-NELTTK--PLSIIITHHNLSNYSSASHMTLSIVEKLWKAVEKKYSCLLSRL--QISCKNPESSTKFKNICCIPIFVLVIIFFSAIIINATLFTIYNKNPMDIFVNKGLLPLFVIMTCIVFLCIFGTLPLVCRT----ITFLVRSPSRQLKQFMES-ENIDFQASDTLVKNELLKLCQIISIFEAYLNSEKIRMFVFIDAADTESREKLANSIYLVNGLLQMSSNAPIALILAADFQTLNTEWXXXXXXXXXXXXXXXXIFQEISDNFH-LIHSTIHLPIYMEEGIDFNRNSSGKKKY----ASNGMFCIRGGNSFE-------SLNMVTTGTKQNSVPVSTRKTSIYXXXXXXXXXXXXXKGASKSIQQLFLTRGDLSDINNKMIKQLTAAAAFIGRLLRINLIEFKLENLISWLSISINWPYQTALMALFLEEQCANLTPKHTLLQIHEQVLSKIGSKDSNPAEIELLARDDRDFGKLDAFIKKDVGATLTFSILKYFVSWSPFMNPYLRYVLRDYLTNKNLAQTRGSRNQLT-------------SPPLTSMALSDPSDVPMDEDLPQP----IISPSTGSGLVNQITS----------------WSKSSIGNLAHKGDRPKTPIAIKRCMVKLKDGYPGKD----LSQLDKYEIGILMCQIEGLSSEENIEKYKEKLIQLNVTGLVLSVCDMDQLQNEMEMNFGDWQLFKTLIYHLRSVEA 2848
Y ALAD+L P PI +GL+++ G+G ++ L+KL DE+ + + Q S L+ + L +G++ + +G+++ S + ++ +I FG + W A + LA L+L L ++F P E E TTK P+ + T +N + ++ L A E+++ L +RL ++ + K+K CC+P FV+ + II TL I+ +P + VN + + + +V L L CRT + L+ S ++L + + ++K E+ + ++ +++ ++ R+ V ID D ++K+ + +++ + + S P I A+D + + + +++ ++ + N H + + +HLP+++ +R S +K+ A+NG + S N + TK S R+ + + ++ S + +L +T SDI+ + +++L + GRLLR N I F + L SW++++ WPY+T+ + L+LEE + + TL I+E++ I + ++E L D D + F+ L +K F+ + ++P LR ++ D R +R Q++ PP S P V P ++SP S + +T + G H RP ++ R P L+ L+ + + QIEGL + + +Y + + N+ G VL+ C++D+L+ EM MNFGDW LF++ + +R+ E+
Sbjct: 443 YSSALADILSEPTMQPPICVGLYAQWGSG-KSFLLKKLEDEMKTFAGQQIEPLFQFSWLIVFLTLLLCGGLGLLFAFTVHPNLGIAVSLSFLALLYIFFIVIYFGGRREGESWNWAWVLSTRLARHIGYLELLLKLMFVNPPELPEQTTKALPVRFLFTDYNRLSSVGGETSLAEMIATLSDACEREFGFLATRLFRVFKTEDTQGKKKWKKTCCLPSFVIFLFIIGCIISGITLLAIFRVDPKHLTVNA----VLISIASVVGLAFV----LNCRTWWQVLDSLLNSQRKRLHNAASKLHKLKSEGFMKVLKCEVELMARMAKTIDSFTQNQ-TRLVVIIDGLDACEQDKV---LQMLDTVRVLFSKGPFIAIFASDPHII----------IKAINQNLNSVLRDSNINGHDYMRNIVHLPVFLN-----SRGLSNARKFLVTSATNGDVPCSDTTGIQEDADRRVSQNSLGEMTKLGSKTALNRRDTY----RRRQMQRTITRQMSFDLTKLLVTEDWFSDISPQTMRRLLNIVSVTGRLLRANQISFNWDRLASWINLTEQWPYRTSWLILYLEE-TEGIPDQMTLKTIYERISKNIPTT----KDVEPLLEIDGDIRNFEVFLSSRT-PVLVARDVKVFLPCTVNLDPKLREIIAD---------VRAAREQISIGGLAYPPLPLHEGPPRAPSGYSQPPSVCSSTSFNGPFAGGVVSPQPHSSYYSGMTGPQHPFYNRPFFAPYLYTPRYYPGGSQHLISRPSVKTSLPRDQNNGLGSGPAPGPVVLLNSLNVDAVCEKLKQIEGL-DQSMLPQYCTTIKKANINGRVLAQCNIDELKKEMNMNFGDWHLFRSTVLEMRNAES 1262
BLAST of Kinase D-interacting substrate vs. Ensembl Human
Match: KIDINS220 (kinase D interacting substrate 220 [Source:HGNC Symbol;Acc:HGNC:29508]) HSP 1 Score: 173.326 bits (438), Expect = 8.584e-43 Identity = 193/849 (22.73%), Postives = 366/849 (43.11%), Query Frame = 2
Query: 428 YIQALADMLVCPNTNFPISIGLFSRVGAGIRTTHLEKLSDELISLTKINNQKTLQPSLLLFMFPXXXXXXXXXXXXXXTNWIVGVSLGASLSLMSFLLIEIIIFGSNHK--RWGKALRAARWLASFTQSLQLFLDILFRYPHE-NELTTK--PLSIIITHHNLSNYSSASHMTLSIVEKLWKAVEKKYSCLLSRL--QISCKNPESSTKFKNICCIPIFVLVIIFFSAIIINATLFTIYNKNPMDIFVNKGLLPLFVIMTCIVFLCIFGTLPLVCRT----ITFLVRSPSRQLKQFMES-ENIDFQASDTLVKNELLKLCQIISIFEAYLNSEKIRMFVFIDAADTESREKLANSIYLVNGLLQMSSNAPIALILAADFQTLNTEWXXXXXXXXXXXXXXXXIFQEISDNFH-LIHSTIHLPIYMEEGIDFNRNSSGKKKY----ASNGMFCIRGGNSFE-------SLNMVTTGTKQNSVPVSTRKTSIYXXXXXXXXXXXXXKGASKSIQQLFLTRGDLSDINNKMIKQLTAAAAFIGRLLRINLIEFKLENLISWLSISINWPYQTALMALFLEEQCANLTPKHTLLQIHEQVLSKIGSKDSNPAEIELLARDDRDFGKLDAFIKKDVGATLTFSILKYFVSWSPFMNPYLRYVLRDYLTNKNLAQTRGSRNQLT-------------SPPLTSMALSDPSDVPMDEDLPQP----IISPSTGSGLVNQITSWSKSSIGNLAHKGDRPK-TPIAIKRCMVKLKDGYPGKDLSQLDKYEIGILMCQIEGLSSEENIEKYKEKLIQLNVTGLVLSVCDMDQLQNEMEMNFGDWQLFKTLIYHLRSVEA 2848
Y ALAD+L P PI +GL+++ G+G ++ L+KL DE+ + + Q S L+ + L +G++ + +G+++ S + ++ +I FG + W A + LA L+L L ++F P E E TTK P+ + T +N + ++ L A E+++ L +RL ++ + K+K CC+P FV+ + II TL I+ +P + VN L+ + ++ + F+ L CRT + L+ S ++L + + ++K E+ + ++ +++ ++ R+ V ID D ++K+ + V L S P I A+D + + + +++ ++ + N H + + +HLP+++ +R S +K+ A+NG + S N + TK S R+ + + ++ S + +L +T SDI+ + +++L + GRLLR N I F + L SW++++ WPY+T+ + L+LEE + + TL I+E++ I + ++E L D D + F+ L +K F+ + ++P LR ++ D R +R Q++ PP S P V P ++SP S + +T ++G P P+ + L+ L+ + + QIEGL + + +Y + + N+ G VL+ C++D+L+ EM MNFGDW LF++ + +R+ E+
Sbjct: 444 YSSALADILSEPTMQPPICVGLYAQWGSG-KSFLLKKLEDEMKTFAGQQIEPLFQFSWLIVFLTLLLCGGLGLLFAFTVHPNLGIAVSLSFLALLYIFFIVIYFGGRREGESWNWAWVLSTRLARHIGYLELLLKLMFVNPPELPEQTTKALPVRFLFTDYNRLSSVGGETSLAEMIATLSDACEREFGFLATRLFRVFKTEDTQGKKKWKKTCCLPSFVIFLFIIGCIISGITLLAIFRVDPKHLTVNAVLISIASVVG-LAFV-------LNCRTWWQVLDSLLNSQRKRLHNAASKLHKLKSEGFMKVLKCEVELMARMAKTIDSFTQNQ-TRLVVIIDGLDACEQDKVLQMLDTVRVLF---SKGPFIAIFASDPHII----------IKAINQNLNSVLRDSNINGHDYMRNIVHLPVFLN-----SRGLSNARKFLVTSATNGDVPCSDTTGIQEDADRRVSQNSLGEMTKLGSKTALNRRDT----YRRRQMQRTITRQMSFDLTKLLVTEDWFSDISPQTMRRLLNIVSVTGRLLRANQISFNWDRLASWINLTEQWPYRTSWLILYLEE-TEGIPDQMTLKTIYERISKNIPTT----KDVEPLLEIDGDIRNFEVFLSSRT-PVLVARDVKVFLPCTVNLDPKLREIIAD---------VRAAREQISIGGLAYPPLPLHEGPPRAPSGYSQPPSVCSSTSFNGPFAGGVVSPQPHSSYYSGMTGPQHP----FYNRGSGPAPGPVVL---------------LNSLNVDAVCEKLKQIEGL-DQSMLPQYCTTIKKANINGRVLAQCNIDELKKEMNMNFGDWHLFRSTVLEMRNAES 1225
BLAST of Kinase D-interacting substrate vs. Ensembl Human
Match: KIDINS220 (kinase D interacting substrate 220 [Source:HGNC Symbol;Acc:HGNC:29508]) HSP 1 Score: 172.94 bits (437), Expect = 1.057e-42 Identity = 193/849 (22.73%), Postives = 366/849 (43.11%), Query Frame = 2
Query: 428 YIQALADMLVCPNTNFPISIGLFSRVGAGIRTTHLEKLSDELISLTKINNQKTLQPSLLLFMFPXXXXXXXXXXXXXXTNWIVGVSLGASLSLMSFLLIEIIIFGSNHK--RWGKALRAARWLASFTQSLQLFLDILFRYPHE-NELTTK--PLSIIITHHNLSNYSSASHMTLSIVEKLWKAVEKKYSCLLSRL--QISCKNPESSTKFKNICCIPIFVLVIIFFSAIIINATLFTIYNKNPMDIFVNKGLLPLFVIMTCIVFLCIFGTLPLVCRT----ITFLVRSPSRQLKQFMES-ENIDFQASDTLVKNELLKLCQIISIFEAYLNSEKIRMFVFIDAADTESREKLANSIYLVNGLLQMSSNAPIALILAADFQTLNTEWXXXXXXXXXXXXXXXXIFQEISDNFH-LIHSTIHLPIYMEEGIDFNRNSSGKKKY----ASNGMFCIRGGNSFE-------SLNMVTTGTKQNSVPVSTRKTSIYXXXXXXXXXXXXXKGASKSIQQLFLTRGDLSDINNKMIKQLTAAAAFIGRLLRINLIEFKLENLISWLSISINWPYQTALMALFLEEQCANLTPKHTLLQIHEQVLSKIGSKDSNPAEIELLARDDRDFGKLDAFIKKDVGATLTFSILKYFVSWSPFMNPYLRYVLRDYLTNKNLAQTRGSRNQLT-------------SPPLTSMALSDPSDVPMDEDLPQP----IISPSTGSGLVNQITSWSKSSIGNLAHKGDRPK-TPIAIKRCMVKLKDGYPGKDLSQLDKYEIGILMCQIEGLSSEENIEKYKEKLIQLNVTGLVLSVCDMDQLQNEMEMNFGDWQLFKTLIYHLRSVEA 2848
Y ALAD+L P PI +GL+++ G+G ++ L+KL DE+ + + Q S L+ + L +G++ + +G+++ S + ++ +I FG + W A + LA L+L L ++F P E E TTK P+ + T +N + ++ L A E+++ L +RL ++ + K+K CC+P FV+ + II TL I+ +P + VN L+ + ++ + F+ L CRT + L+ S ++L + + ++K E+ + ++ +++ ++ R+ V ID D ++K+ + V L S P I A+D + + + +++ ++ + N H + + +HLP+++ +R S +K+ A+NG + S N + TK S R+ + + ++ S + +L +T SDI+ + +++L + GRLLR N I F + L SW++++ WPY+T+ + L+LEE + + TL I+E++ I + ++E L D D + F+ L +K F+ + ++P LR ++ D R +R Q++ PP S P V P ++SP S + +T ++G P P+ + L+ L+ + + QIEGL + + +Y + + N+ G VL+ C++D+L+ EM MNFGDW LF++ + +R+ E+
Sbjct: 443 YSSALADILSEPTMQPPICVGLYAQWGSG-KSFLLKKLEDEMKTFAGQQIEPLFQFSWLIVFLTLLLCGGLGLLFAFTVHPNLGIAVSLSFLALLYIFFIVIYFGGRREGESWNWAWVLSTRLARHIGYLELLLKLMFVNPPELPEQTTKALPVRFLFTDYNRLSSVGGETSLAEMIATLSDACEREFGFLATRLFRVFKTEDTQGKKKWKKTCCLPSFVIFLFIIGCIISGITLLAIFRVDPKHLTVNAVLISIASVVG-LAFV-------LNCRTWWQVLDSLLNSQRKRLHNAASKLHKLKSEGFMKVLKCEVELMARMAKTIDSFTQNQ-TRLVVIIDGLDACEQDKVLQMLDTVRVLF---SKGPFIAIFASDPHII----------IKAINQNLNSVLRDSNINGHDYMRNIVHLPVFLN-----SRGLSNARKFLVTSATNGDVPCSDTTGIQEDADRRVSQNSLGEMTKLGSKTALNRRDTY----RRRQMQRTITRQMSFDLTKLLVTEDWFSDISPQTMRRLLNIVSVTGRLLRANQISFNWDRLASWINLTEQWPYRTSWLILYLEE-TEGIPDQMTLKTIYERISKNIPTTK----DVEPLLEIDGDIRNFEVFLSSRT-PVLVARDVKVFLPCTVNLDPKLREIIAD---------VRAAREQISIGGLAYPPLPLHEGPPRAPSGYSQPPSVCSSTSFNGPFAGGVVSPQPHSSYYSGMTGPQHP----FYNRGSGPAPGPVVL---------------LNSLNVDAVCEKLKQIEGL-DQSMLPQYCTTIKKANINGRVLAQCNIDELKKEMNMNFGDWHLFRSTVLEMRNAES 1224
BLAST of Kinase D-interacting substrate vs. Ensembl Human
Match: KIDINS220 (kinase D interacting substrate 220 [Source:HGNC Symbol;Acc:HGNC:29508]) HSP 1 Score: 167.933 bits (424), Expect = 4.583e-41 Identity = 196/887 (22.10%), Postives = 372/887 (41.94%), Query Frame = 2
Query: 428 YIQALADMLVCPNTNFPISIGLFSRVGAGIRTTHLEKLSDELISLTKINNQKTLQPSLLLFMFPXXXXXXXXXXXXXXTNWIVGVSLGASLSLMSFLLIEIIIFGSNHK--RWGKALRAARWLASFTQSLQLFLDILFRYPHE-NELTTK--PLSIIITHHNLSNYSSASHMTLSIVEKLWKAVEKKYSCLLSRL--QISCKNPESSTKFKNICCIPIFVLVIIFFSAIIINATLFTIYNKNPMDIFVNKGLLPLFVIMTCIVFLCIFGTLPLVCRT----ITFLVRSPSRQLKQFMES-ENIDFQASDTLVKNELLKLCQIISIFEAYLNSEKIRMFVFIDAADTESREKLANSIYLVNGLLQMSSNAPIALILAADFQTLNTEWXXXXXXXXXXXXXXXXIFQEISDNFH-LIHSTIHLPIYMEEGIDFNRNSSGKKKY----ASNGMFCIRGGNSFE-------SLNMVTTGTKQNSVPVSTRKTSIYXXXXXXXXXXXXXKGASKSIQQLFLTRGDLSDINNKMIKQLTAAAAFIGRLLRINLIEFKLENLISWLSISINWPYQTALMALFLEEQCANLTPKHTLLQIHEQVLSKIGSKDSNPAEIELLARDDRDFGKLDAFIKKDVGATLTFSILKYFVSWSPFMNPYLRYVLRDYLTNKNLAQTRGSRNQLT-------------SPPLTSMALSDPSDVPMDEDLPQP----IISPSTGSGLVNQITS----------------WSKSSIGNLAHKGDRPKTPIAIKR-----CMVKLKDGYPGKD------------------LSQLDKYEIGILMCQIEGLSSEENIEKYKEKLIQLNVTGLVLSVCDMDQLQNEMEMNFGDWQLFKTLIYHLRSVEA 2848
Y ALAD+L P PI +GL+++ G+G ++ L+KL DE+ + + Q S L+ + L +G++ + +G+++ S + ++ +I FG + W A + LA L+L L ++F P E E TTK P+ + T +N + ++ L A E+++ L +RL ++ + K+K CC+P FV+ + II TL I+ +P + VN + + + +V L L CRT + L+ S ++L + + ++K E+ + ++ +++ ++ R+ V ID D ++K+ + +++ + + S P I A+D + + + +++ ++ + N H + + +HLP+++ +R S +K+ A+NG + S N + TK S R+ + + ++ S + +L +T SDI+ + +++L + GRLLR N I F + L SW++++ WPY+T+ + L+LEE + + TL I+E++ I + ++E L D D + F+ L +K F+ + ++P LR ++ D R +R Q++ PP S P V P ++SP S + +T + G H RP ++ R V +D G L+ L+ + + QIEGL + + +Y + + N+ G VL+ C++D+L+ EM MNFGDW LF++ + +R+ E+
Sbjct: 443 YSSALADILSEPTMQPPICVGLYAQWGSG-KSFLLKKLEDEMKTFAGQQIEPLFQFSWLIVFLTLLLCGGLGLLFAFTVHPNLGIAVSLSFLALLYIFFIVIYFGGRREGESWNWAWVLSTRLARHIGYLELLLKLMFVNPPELPEQTTKALPVRFLFTDYNRLSSVGGETSLAEMIATLSDACEREFGFLATRLFRVFKTEDTQGKKKWKKTCCLPSFVIFLFIIGCIISGITLLAIFRVDPKHLTVNA----VLISIASVVGLAFV----LNCRTWWQVLDSLLNSQRKRLHNAASKLHKLKSEGFMKVLKCEVELMARMAKTIDSFTQNQ-TRLVVIIDGLDACEQDKV---LQMLDTVRVLFSKGPFIAIFASDPHII----------IKAINQNLNSVLRDSNINGHDYMRNIVHLPVFLN-----SRGLSNARKFLVTSATNGDVPCSDTTGIQEDADRRVSQNSLGEMTKLGSKTALNRRDTY----RRRQMQRTITRQMSFDLTKLLVTEDWFSDISPQTMRRLLNIVSVTGRLLRANQISFNWDRLASWINLTEQWPYRTSWLILYLEE-TEGIPDQMTLKTIYERISKNIPTT----KDVEPLLEIDGDIRNFEVFLSSRT-PVLVARDVKVFLPCTVNLDPKLREIIAD---------VRAAREQISIGGLAYPPLPLHEGPPRAPSGYSQPPSVCSSTSFNGPFAGGVVSPQPHSSYYSGMTGPQHPFYNRPFFAPYLYTPRYYPGGSQHLISRPSVKTSLPRDQNNGLEVIKEDAAEGLSSPTDSSRGSGPAPGPVVLLNSLNVDAVCEKLKQIEGL-DQSMLPQYCTTIKKANINGRVLAQCNIDELKKEMNMNFGDWHLFRSTVLEMRNAES 1281
BLAST of Kinase D-interacting substrate vs. Ensembl Celegans
Match: kdin-1 (KiDINs220 (Vertebrate scaffold protein) homolog [Source:UniProtKB/TrEMBL;Acc:A0A061AD72]) HSP 1 Score: 65.0846 bits (157), Expect = 3.907e-10 Identity = 113/602 (18.77%), Postives = 232/602 (38.54%), Query Frame = 2
Query: 380 PLDHCGSNIRAQKTVTYIQALADMLVCPNTNFPISIGLFSRVGAGIRTTHLEKLSDELISLTK---------INNQKTLQPSLLLFMFPXXXXXXXXXXXXXXTNWIVGVSLGASLSLMSFLL---IEIIIFGSNHKRWGKALRAARWLASFTQSLQLFLDIL-FRYPHENELTTK-PLSIIIT-HHNLSNYSSASHMTLSIVEKLWKAVEKKYSCLLSRLQISCKNPESS--TKFKNICCIPIFVLVIIFFSAIIINATLFTIY---NKNPMDIFVNKGLLPLFVIMTCIVFLCIF---GTLPLVCRTITFLVRSPSRQLKQFME-SENIDFQASDTLVKNELLKLCQIISIFEAYLNSEKIRMFVFIDAADTESREKLANSIYLVNGLLQMSSNAPIALILAADFQTLNTEWXXXXXXXXXXXXXXXXIFQEISDNFH------------LIHSTIHLPIYMEEGIDFNRNSSGKKKYASNGMFCIR--GGNSFESLNMV------TTGTKQNSVPVSTRKTSIYXXXXXXXXXXXXXKGASKSIQQLFLTRGDLSDINNKMIKQLTAAAAFIGRLLRINLIEFKLENLISWLSISINWP 2053
P+D A Y LAD++ P+ + P++IGL+++ G+G ++ L KL + + S ++ ++ L MF + ++ +N + + S+ L+ F++ + ++++ + K W ++ A + A ++L +IL P +E + P+S + +H LS+ + IV L++A E + L RL K P + C +P +L+I+ +I+ T++ +++P + F + I FLC F PL + P R++ + + F+ ++ E+ L +I +A+ S R+ V +D D +E++ ++ + L + P I+A D I I+ N H + + I +P Y+ S ++K S + R ++F ++ T K+++VP + + +++ L S++N + ++++ A GRL+R I+F +L W+S+ WP
Sbjct: 451 PIDAEDKMDTAMGYDVYSNVLADIVCEPSLSLPLTIGLYAKWGSG-KSALLAKLKEAMHSFSRDWLDGVSLSVSFALFFAIFLFFGMF-----SLTFTMLIAISNSVTAYLISWSVFLLIFIIFCSLIVVVYYGDRKNWYTSMDIANFFARVFSRIRLVYNILTLHAPMNSEDSASMPVSFLFADYHRLSSIGGEQALA-KIVATLFEAAETHFGVLPVRLFCCMKPPYPGIHGSLRRHCGVPHVILLIVAVFLLIMAQVFGTVWLLSDRDPNN----------FNLFIAIAFLCGFVMIAIYPLALIIMYSWTNVPRRRVNAAARNAHKLRFEGLMQKLQTEVDLLADMIRSLDAFTRSH-TRLVVVVDGLDNCEQERMVQTLDALELLFSARKHRPFITIIAVDPHV---------------------IVSAINHNMHSALSGTELTGHDYLKNIISMPFYLHNSALRQLQSKLREKRESMAEWKERFKRQDTFYGSHLSLREADGRTSRKKSTVPNMNSNSVV-----------------GRNMNDGILGEDYFSNMNPRAMRRIVNALTLTGRLMRAFEIDFSWMSLGHWVSLLEQWP 996
BLAST of Kinase D-interacting substrate vs. Ensembl Celegans
Match: kdin-1 (KiDINs220 (Vertebrate scaffold protein) homolog [Source:UniProtKB/TrEMBL;Acc:A0A061AD72]) HSP 1 Score: 65.0846 bits (157), Expect = 4.254e-10 Identity = 114/596 (19.13%), Postives = 233/596 (39.09%), Query Frame = 2
Query: 380 PLDHCGSNIRAQKTVTYIQALADMLVCPNTNFPISIGLFSRVGAGIRTTHLEKLSDELISLTK---------INNQKTLQPSLLLFMFPXXXXXXXXXXXXXXTNWIVGVSLGASLSLMSFLL---IEIIIFGSNHKRWGKALRAARWLASFTQSLQLFLDIL-FRYPHENELTTK-PLSIIIT-HHNLSNYSSASHMTLSIVEKLWKAVEKKYSCLLSRLQISCKNPESS--TKFKNICCIPIFVLVIIFFSAIIINATLFTIY---NKNPMDIFVNKGLLPLFVIMTCIVFLCIF---GTLPLVCRTITFLVRSPSRQLKQFME-SENIDFQASDTLVKNELLKLCQIISIFEAYLNSEKIRMFVFIDAADTESREKLANSIYLVNGLLQMSSNAPIALILAADFQTLNTEWXXXXXXXXXXXXXXXXIFQEISDNFH------------LIHSTIHLPIYMEEGIDFNRNSSGKKKYASNGMFCIR--GGNSFESLNMVTTGTKQNSVPVSTRKTSIYXXXXXXXXXXXXXKGASKSIQQLFLTRGDLSDINNKMIKQLTAAAAFIGRLLRINLIEFKLENLISWLSISINWP 2053
P+D A Y LAD++ P+ + P++IGL+++ G+G ++ L KL + + S ++ ++ L MF + ++ +N + + S+ L+ F++ + ++++ + K W ++ A + A ++L +IL P +E + P+S + +H LS+ + IV L++A E + L RL K P + C +P +L+I+ +I+ T++ +++P + F + I FLC F PL + P R++ + + F+ ++ E+ L +I +A+ S R+ V +D D +E++ ++ + L + P I+A D I I+ N H + + I +P Y+ S ++K S + R ++F ++ + S +K+++ NS+ + + G L S++N + ++++ A GRL+R I+F +L W+S+ WP
Sbjct: 451 PIDAEDKMDTAMGYDVYSNVLADIVCEPSLSLPLTIGLYAKWGSG-KSALLAKLKEAMHSFSRDWLDGVSLSVSFALFFAIFLFFGMF-----SLTFTMLIAISNSVTAYLISWSVFLLIFIIFCSLIVVVYYGDRKNWYTSMDIANFFARVFSRIRLVYNILTLHAPMNSEDSASMPVSFLFADYHRLSSIGGEQALA-KIVATLFEAAETHFGVLPVRLFCCMKPPYPGIHGSLRRHCGVPHVILLIVAVFLLIMAQVFGTVWLLSDRDPNN----------FNLFIAIAFLCGFVMIAIYPLALIIMYSWTNVPRRRVNAAARNAHKLRFEGLMQKLQTEVDLLADMIRSLDAFTRSH-TRLVVVVDGLDNCEQERMVQTLDALELLFSARKHRPFITIIAVDPHV---------------------IVSAINHNMHSALSGTELTGHDYLKNIISMPFYLHNSALRQLQSKLREKRESMAEWKERFKRQDTFYGSHLSLREADGRTSRKSFQKSTVPNMNSNSVVGRNMNDG--------ILGEDYFSNMNPRAMRRIVNALTLTGRLMRAFEIDFSWMSLGHWVSLLEQWP 999
BLAST of Kinase D-interacting substrate vs. Ensembl Celegans
Match: kdin-1 (KiDINs220 (Vertebrate scaffold protein) homolog [Source:UniProtKB/TrEMBL;Acc:A0A061AD72]) HSP 1 Score: 64.6994 bits (156), Expect = 6.028e-10 Identity = 113/602 (18.77%), Postives = 232/602 (38.54%), Query Frame = 2
Query: 380 PLDHCGSNIRAQKTVTYIQALADMLVCPNTNFPISIGLFSRVGAGIRTTHLEKLSDELISLTK---------INNQKTLQPSLLLFMFPXXXXXXXXXXXXXXTNWIVGVSLGASLSLMSFLL---IEIIIFGSNHKRWGKALRAARWLASFTQSLQLFLDIL-FRYPHENELTTK-PLSIIIT-HHNLSNYSSASHMTLSIVEKLWKAVEKKYSCLLSRLQISCKNPESS--TKFKNICCIPIFVLVIIFFSAIIINATLFTIY---NKNPMDIFVNKGLLPLFVIMTCIVFLCIF---GTLPLVCRTITFLVRSPSRQLKQFME-SENIDFQASDTLVKNELLKLCQIISIFEAYLNSEKIRMFVFIDAADTESREKLANSIYLVNGLLQMSSNAPIALILAADFQTLNTEWXXXXXXXXXXXXXXXXIFQEISDNFH------------LIHSTIHLPIYMEEGIDFNRNSSGKKKYASNGMFCIR--GGNSFESLNMV------TTGTKQNSVPVSTRKTSIYXXXXXXXXXXXXXKGASKSIQQLFLTRGDLSDINNKMIKQLTAAAAFIGRLLRINLIEFKLENLISWLSISINWP 2053
P+D A Y LAD++ P+ + P++IGL+++ G+G ++ L KL + + S ++ ++ L MF + ++ +N + + S+ L+ F++ + ++++ + K W ++ A + A ++L +IL P +E + P+S + +H LS+ + IV L++A E + L RL K P + C +P +L+I+ +I+ T++ +++P + F + I FLC F PL + P R++ + + F+ ++ E+ L +I +A+ S R+ V +D D +E++ ++ + L + P I+A D I I+ N H + + I +P Y+ S ++K S + R ++F ++ T K+++VP + + +++ L S++N + ++++ A GRL+R I+F +L W+S+ WP
Sbjct: 451 PIDAEDKMDTAMGYDVYSNVLADIVCEPSLSLPLTIGLYAKWGSG-KSALLAKLKEAMHSFSRDWLDGVSLSVSFALFFAIFLFFGMF-----SLTFTMLIAISNSVTAYLISWSVFLLIFIIFCSLIVVVYYGDRKNWYTSMDIANFFARVFSRIRLVYNILTLHAPMNSEDSASMPVSFLFADYHRLSSIGGEQALA-KIVATLFEAAETHFGVLPVRLFCCMKPPYPGIHGSLRRHCGVPHVILLIVAVFLLIMAQVFGTVWLLSDRDPNN----------FNLFIAIAFLCGFVMIAIYPLALIIMYSWTNVPRRRVNAAARNAHKLRFEGLMQKLQTEVDLLADMIRSLDAFTRSH-TRLVVVVDGLDNCEQERMVQTLDALELLFSARKHRPFITIIAVDPHV---------------------IVSAINHNMHSALSGTELTGHDYLKNIISMPFYLHNSALRQLQSKLREKRESMAEWKERFKRQDTFYGSHLSLREADGRTSRKKSTVPNMNSNSVV-----------------GRNMNDGILGEDYFSNMNPRAMRRIVNALTLTGRLMRAFEIDFSWMSLGHWVSLLEQWP 996
BLAST of Kinase D-interacting substrate vs. Ensembl Celegans
Match: kdin-1 (KiDINs220 (Vertebrate scaffold protein) homolog [Source:UniProtKB/TrEMBL;Acc:A0A061AD72]) HSP 1 Score: 64.3142 bits (155), Expect = 6.674e-10 Identity = 114/596 (19.13%), Postives = 233/596 (39.09%), Query Frame = 2
Query: 380 PLDHCGSNIRAQKTVTYIQALADMLVCPNTNFPISIGLFSRVGAGIRTTHLEKLSDELISLTK---------INNQKTLQPSLLLFMFPXXXXXXXXXXXXXXTNWIVGVSLGASLSLMSFLL---IEIIIFGSNHKRWGKALRAARWLASFTQSLQLFLDIL-FRYPHENELTTK-PLSIIIT-HHNLSNYSSASHMTLSIVEKLWKAVEKKYSCLLSRLQISCKNPESS--TKFKNICCIPIFVLVIIFFSAIIINATLFTIY---NKNPMDIFVNKGLLPLFVIMTCIVFLCIF---GTLPLVCRTITFLVRSPSRQLKQFME-SENIDFQASDTLVKNELLKLCQIISIFEAYLNSEKIRMFVFIDAADTESREKLANSIYLVNGLLQMSSNAPIALILAADFQTLNTEWXXXXXXXXXXXXXXXXIFQEISDNFH------------LIHSTIHLPIYMEEGIDFNRNSSGKKKYASNGMFCIR--GGNSFESLNMVTTGTKQNSVPVSTRKTSIYXXXXXXXXXXXXXKGASKSIQQLFLTRGDLSDINNKMIKQLTAAAAFIGRLLRINLIEFKLENLISWLSISINWP 2053
P+D A Y LAD++ P+ + P++IGL+++ G+G ++ L KL + + S ++ ++ L MF + ++ +N + + S+ L+ F++ + ++++ + K W ++ A + A ++L +IL P +E + P+S + +H LS+ + IV L++A E + L RL K P + C +P +L+I+ +I+ T++ +++P + F + I FLC F PL + P R++ + + F+ ++ E+ L +I +A+ S R+ V +D D +E++ ++ + L + P I+A D I I+ N H + + I +P Y+ S ++K S + R ++F ++ + S +K+++ NS+ + + G L S++N + ++++ A GRL+R I+F +L W+S+ WP
Sbjct: 451 PIDAEDKMDTAMGYDVYSNVLADIVCEPSLSLPLTIGLYAKWGSG-KSALLAKLKEAMHSFSRDWLDGVSLSVSFALFFAIFLFFGMF-----SLTFTMLIAISNSVTAYLISWSVFLLIFIIFCSLIVVVYYGDRKNWYTSMDIANFFARVFSRIRLVYNILTLHAPMNSEDSASMPVSFLFADYHRLSSIGGEQALA-KIVATLFEAAETHFGVLPVRLFCCMKPPYPGIHGSLRRHCGVPHVILLIVAVFLLIMAQVFGTVWLLSDRDPNN----------FNLFIAIAFLCGFVMIAIYPLALIIMYSWTNVPRRRVNAAARNAHKLRFEGLMQKLQTEVDLLADMIRSLDAFTRSH-TRLVVVVDGLDNCEQERMVQTLDALELLFSARKHRPFITIIAVDPHV---------------------IVSAINHNMHSALSGTELTGHDYLKNIISMPFYLHNSALRQLQSKLREKRESMAEWKERFKRQDTFYGSHLSLREADGRTSRKSFQKSTVPNMNSNSVVGRNMNDG--------ILGEDYFSNMNPRAMRRIVNALTLTGRLMRAFEIDFSWMSLGHWVSLLEQWP 999
BLAST of Kinase D-interacting substrate vs. Ensembl Celegans
Match: kdin-1 (KiDINs220 (Vertebrate scaffold protein) homolog [Source:UniProtKB/TrEMBL;Acc:A0A061AD72]) HSP 1 Score: 56.6102 bits (135), Expect = 1.787e-7 Identity = 116/625 (18.56%), Postives = 239/625 (38.24%), Query Frame = 2
Query: 380 PLDHCGSNIRAQKTVTYIQALADMLVCPNTNFPISIGLFSRVGAGIRTTHLEKLSDELISLTK---------INNQKTLQPSLLLFMFPXXXXXXXXXXXXXXTNWIVGVSLGASLSLMSFLL---IEIIIFGSNHKRWGKALRAARWLASFTQSLQLFLDIL-FRYPHENELTTK-PLSIIIT-HHNLSNYSSASHMTLSIVEKLWKAVEKKYSCLLSRLQISCK-------------NPESSTKFKNI------------------CCIPIFVLVIIFFSAIIINATLFTIY---NKNPMDIFVNKGLLPLFVIMTCIVFLCIF---GTLPLVCRTITFLVRSPSRQLKQFME-SENIDFQASDTLVKNELLKLCQIISIFEAYLNSEKIRMFVFIDAADTESREKLANSIYLVNGLLQMSSNAPIALILAADFQTLNTEWXXXXXXXXXXXXXXXXIFQEISDNFH------------LIHSTIHLPIYMEEGIDFNRNSSGKKKYASNGMFCIR--GGNSFESLNMVTTGTKQNSVPVSTRKTSIYXXXXXXXXXXXXXKGASKSIQQLFLTRGDLSDINNKMIKQLTAAAAFIGRLLRINLIEFKLENLISWLSISINWP 2053
P+D A Y LAD++ P+ + P++IGL+++ G+G ++ L KL + + S ++ ++ L MF + ++ +N + + S+ L+ F++ + ++++ + K W ++ A + A ++L +IL P +E + P+S + +H LS+ + IV L++A E + L RL K +P S T ++++ C +P +L+I+ +I+ T++ +++P + F + I FLC F PL + P R++ + + F+ ++ E+ L +I +A+ S R+ V +D D +E++ ++ + L + P I+A D I I+ N H + + I +P Y+ S ++K S + R ++F ++ + S +K+++ NS+ + + G L S++N + ++++ A GRL+R I+F +L W+S+ WP
Sbjct: 451 PIDAEDKMDTAMGYDVYSNVLADIVCEPSLSLPLTIGLYAKWGSG-KSALLAKLKEAMHSFSRDWLDGVSLSVSFALFFAIFLFFGMF-----SLTFTMLIAISNSVTAYLISWSVFLLIFIIFCSLIVVVYYGDRKNWYTSMDIANFFARVFSRIRLVYNILTLHAPMNSEDSASMPVSFLFADYHRLSSIGGEQALA-KIVATLFEAAETHFGVLPVRLFCCMKPPYQYIDTGFGFTSPFSRTTYRSLQQPNAHQQAGIHGSLRRHCGVPHVILLIVAVFLLIMAQVFGTVWLLSDRDPNN----------FNLFIAIAFLCGFVMIAIYPLALIIMYSWTNVPRRRVNAAARNAHKLRFEGLMQKLQTEVDLLADMIRSLDAFTRSH-TRLVVVVDGLDNCEQERMVQTLDALELLFSARKHRPFITIIAVDPHV---------------------IVSAINHNMHSALSGTELTGHDYLKNIISMPFYLHNSALRQLQSKLREKRESMAEWKERFKRQDTFYGSHLSLREADGRTSRKSFQKSTVPNMNSNSVVGRNMNDG--------ILGEDYFSNMNPRAMRRIVNALTLTGRLMRAFEIDFSWMSLGHWVSLLEQWP 1028
BLAST of Kinase D-interacting substrate vs. Ensembl Fly
Match: CG42672 (gene:FBgn0261554 transcript:FBtr0302707) HSP 1 Score: 133.265 bits (334), Expect = 7.570e-31 Identity = 193/855 (22.57%), Postives = 368/855 (43.04%), Query Frame = 2
Query: 428 YIQALADMLVCPNTNFPISIGLFSRVGAGIRTTHLEKLSDELISLTKINNQKTLQPSLLLFMFPXXXXXXXXXXXXXXTNW--IVGVSLGASLSLMSFLLIEIIIFGSNHKRWGKALRAARWLASFTQSLQLFLDILFRYP--HENELTTKPLSIIITHHNLSNYS---SASHMTLSIVEKLWKAVEKKYSCLLSRL----QISCKNPESSTKFKNICCIPIFVLVIIFFSAIIINATLFTIYNKNPMDIFVNKGLLPLFVIMTCI-VFLCIFGTLPLVCRTITFLVRSPSRQLKQFMESENIDFQASDTLVKNELLKLCQIISIFEAYLNSEKIRMFVFIDAADTESREKLANSIYLVNGLLQMSSNAPIALILAADFQTLNTEWXXXXXXXXXXXXXXXXIFQEISDNFH-LIHSTIHLPIYMEEGIDFNRNSSGKKKYASNGM----FCIRGGNSFESLNMVTTGTKQNSVPVSTRKTSIYXXXXXXXXXXXXXKGASKSIQ---------------------------QLFLTRGDLSDINNKMIKQLTAAAAFIGRLLRINLIEFKLENLISWLSISINWPYQTALMALFLEE-QCANLTPKHTLLQIHEQVLSKIGSKDSNPAEIELLARDDRDFGKLDAFIKKDVGATLTFSILKYFVSWSPFMNPYLRYVLRDYLTNKNLAQTRGSRNQLTSPPLTSMALSDPSDVPMDEDLPQPIISPS----TGSGLVNQITSWSKSSIGNLAHKGDRPKTPIAIKRCMVKLKDGYPGKDLSQLDKYEIGILMCQIEGLSSEENIEKYKEKLIQLNVTGLVLSVCDMDQLQNEMEMNFGDWQLFKTLIYHLRSVE 2845
Y ALAD+L P PI++GL+++ G+G ++ L KL DE+ + + + ++ S LLF+ + + +++G IVGL T W +VGVS L+++LL+ + + + A L L+L L + F +P +++ KP+ N ++ + + +HM ++++ A+E Y L +RL + C + +++ +CCIPI ++ + ++ +L Y + L+ L+VI + +C L ++ + L S R LK+ + S A T++ E+ + ++ +A+ N + R+ IDA D+ E++ + V LL S N P L+++ D + + + +F E H + + +HLP+Y++ +SG +K M F GG +++ + T G +SV + + + G K ++ ++ LT SD+N + +++L RLL+ IEF L SW++++ WP + +++ L ++ +N +L ++E++ K+ +EL DRD KLDAF++ + L + L+ F+ ++ ++PYLR VL++ ++ + GS P +++ P+ +P P P N++ S + S+ P TP+ I D L+ L + L+ +IE + + + K L + + G VL CDM L++ + ++FG W+LF+ LI LR E
Sbjct: 518 YSSALADVLSEPTLTTPITVGLYAKWGSG-KSFLLNKLRDEMNNFARQWAEPPIRTSGLLFIVCLHVALLIGTIVGLST-WSAVVGVSAAVGFLLLAYLLLAAVRYCNYQMDMQWAYSVQHGLEKRMTRLRLILQVAFCHPPGPQSDSQAKPVRFHFAEANSASPTGDGAVAHMLAALLD----AIESHYGWLATRLYRAFRPKCLKVDVGWRWRRMCCIPIVLIFELALVTVVTGISLTVAYFTFADEKEKEHILVALYVIAAVMGTLICTH--LHVLAKVFVSLFTSHIRVLKRAVRSSE---SAPLTMLGAEVAVMTDMVKCLDAFTNQQS-RLVGVIDALDSCDTERILTLLNAVQTLLS-SPNRPFVLLISVDPHVI----------AKAAEANSRRLFTEGGIGGHDFLRNLVHLPVYLQ--------NSGLRKVQRAQMTALLFKRSGGGDYQTDDGPTLG---HSVSARRLSNASEIISSQEKLRGPARGGGGKKLRLSESVASSTGSNLHRLGQNPQTVLDLSRIVLTDDYFSDVNPRSMRRLMNVIYITVRLLKAFQIEFSWYRLSSWINLTEQWPLRASMIVLHHDQFMDSNADESVSLQSVYEKLRPKLAYLREAAPLLEL----DRDERKLDAFLQLH-KSDLLVADLRIFLPFTINLDPYLRKVLKE---DQQTIEDEGSLVIQARPSVSNTMRQFPAPTTY---VPSPQAYPPYQMFQNEYPANELRSRNLST-------STEPVTPL-INSPSDSFGDDILQTKLTDLTVEGVISLLDRIEDM--KPALPKLAPVLRENAINGRVLKHCDMPDLKSVLGLSFGHWELFRLLITTLRECE 1317
BLAST of Kinase D-interacting substrate vs. Ensembl Fly
Match: CG42672 (gene:FBgn0261554 transcript:FBtr0302703) HSP 1 Score: 133.265 bits (334), Expect = 7.759e-31 Identity = 193/855 (22.57%), Postives = 368/855 (43.04%), Query Frame = 2
Query: 428 YIQALADMLVCPNTNFPISIGLFSRVGAGIRTTHLEKLSDELISLTKINNQKTLQPSLLLFMFPXXXXXXXXXXXXXXTNW--IVGVSLGASLSLMSFLLIEIIIFGSNHKRWGKALRAARWLASFTQSLQLFLDILFRYP--HENELTTKPLSIIITHHNLSNYS---SASHMTLSIVEKLWKAVEKKYSCLLSRL----QISCKNPESSTKFKNICCIPIFVLVIIFFSAIIINATLFTIYNKNPMDIFVNKGLLPLFVIMTCI-VFLCIFGTLPLVCRTITFLVRSPSRQLKQFMESENIDFQASDTLVKNELLKLCQIISIFEAYLNSEKIRMFVFIDAADTESREKLANSIYLVNGLLQMSSNAPIALILAADFQTLNTEWXXXXXXXXXXXXXXXXIFQEISDNFH-LIHSTIHLPIYMEEGIDFNRNSSGKKKYASNGM----FCIRGGNSFESLNMVTTGTKQNSVPVSTRKTSIYXXXXXXXXXXXXXKGASKSIQ---------------------------QLFLTRGDLSDINNKMIKQLTAAAAFIGRLLRINLIEFKLENLISWLSISINWPYQTALMALFLEE-QCANLTPKHTLLQIHEQVLSKIGSKDSNPAEIELLARDDRDFGKLDAFIKKDVGATLTFSILKYFVSWSPFMNPYLRYVLRDYLTNKNLAQTRGSRNQLTSPPLTSMALSDPSDVPMDEDLPQPIISPS----TGSGLVNQITSWSKSSIGNLAHKGDRPKTPIAIKRCMVKLKDGYPGKDLSQLDKYEIGILMCQIEGLSSEENIEKYKEKLIQLNVTGLVLSVCDMDQLQNEMEMNFGDWQLFKTLIYHLRSVE 2845
Y ALAD+L P PI++GL+++ G+G ++ L KL DE+ + + + ++ S LLF+ + + +++G IVGL T W +VGVS L+++LL+ + + + A L L+L L + F +P +++ KP+ N ++ + + +HM ++++ A+E Y L +RL + C + +++ +CCIPI ++ + ++ +L Y + L+ L+VI + +C L ++ + L S R LK+ + S A T++ E+ + ++ +A+ N + R+ IDA D+ E++ + V LL S N P L+++ D + + + +F E H + + +HLP+Y++ +SG +K M F GG +++ + T G +SV + + + G K ++ ++ LT SD+N + +++L RLL+ IEF L SW++++ WP + +++ L ++ +N +L ++E++ K+ +EL DRD KLDAF++ + L + L+ F+ ++ ++PYLR VL++ ++ + GS P +++ P+ +P P P N++ S + S+ P TP+ I D L+ L + L+ +IE + + + K L + + G VL CDM L++ + ++FG W+LF+ LI LR E
Sbjct: 518 YSSALADVLSEPTLTTPITVGLYAKWGSG-KSFLLNKLRDEMNNFARQWAEPPIRTSGLLFIVCLHVALLIGTIVGLST-WSAVVGVSAAVGFLLLAYLLLAAVRYCNYQMDMQWAYSVQHGLEKRMTRLRLILQVAFCHPPGPQSDSQAKPVRFHFAEANSASPTGDGAVAHMLAALLD----AIESHYGWLATRLYRAFRPKCLKVDVGWRWRRMCCIPIVLIFELALVTVVTGISLTVAYFTFADEKEKEHILVALYVIAAVMGTLICTH--LHVLAKVFVSLFTSHIRVLKRAVRSSE---SAPLTMLGAEVAVMTDMVKCLDAFTNQQS-RLVGVIDALDSCDTERILTLLNAVQTLLS-SPNRPFVLLISVDPHVI----------AKAAEANSRRLFTEGGIGGHDFLRNLVHLPVYLQ--------NSGLRKVQRAQMTALLFKRSGGGDYQTDDGPTLG---HSVSARRLSNASEIISSQEKLRGPARGGGGKKLRLSESVASSTGSNLHRLGQNPQTVLDLSRIVLTDDYFSDVNPRSMRRLMNVIYITVRLLKAFQIEFSWYRLSSWINLTEQWPLRASMIVLHHDQFMDSNADESVSLQSVYEKLRPKLAYLREAAPLLEL----DRDERKLDAFLQLH-KSDLLVADLRIFLPFTINLDPYLRKVLKE---DQQTIEDEGSLVIQARPSVSNTMRQFPAPTTY---VPSPQAYPPYQMFQNEYPANELRSRNLST-------STEPVTPL-INSPSDSFGDDILQTKLTDLTVEGVISLLDRIEDM--KPALPKLAPVLRENAINGRVLKHCDMPDLKSVLGLSFGHWELFRLLITTLRECE 1317
BLAST of Kinase D-interacting substrate vs. Ensembl Fly
Match: CG42672 (gene:FBgn0261554 transcript:FBtr0342886) HSP 1 Score: 133.265 bits (334), Expect = 9.547e-31 Identity = 193/855 (22.57%), Postives = 368/855 (43.04%), Query Frame = 2
Query: 428 YIQALADMLVCPNTNFPISIGLFSRVGAGIRTTHLEKLSDELISLTKINNQKTLQPSLLLFMFPXXXXXXXXXXXXXXTNW--IVGVSLGASLSLMSFLLIEIIIFGSNHKRWGKALRAARWLASFTQSLQLFLDILFRYP--HENELTTKPLSIIITHHNLSNYS---SASHMTLSIVEKLWKAVEKKYSCLLSRL----QISCKNPESSTKFKNICCIPIFVLVIIFFSAIIINATLFTIYNKNPMDIFVNKGLLPLFVIMTCI-VFLCIFGTLPLVCRTITFLVRSPSRQLKQFMESENIDFQASDTLVKNELLKLCQIISIFEAYLNSEKIRMFVFIDAADTESREKLANSIYLVNGLLQMSSNAPIALILAADFQTLNTEWXXXXXXXXXXXXXXXXIFQEISDNFH-LIHSTIHLPIYMEEGIDFNRNSSGKKKYASNGM----FCIRGGNSFESLNMVTTGTKQNSVPVSTRKTSIYXXXXXXXXXXXXXKGASKSIQ---------------------------QLFLTRGDLSDINNKMIKQLTAAAAFIGRLLRINLIEFKLENLISWLSISINWPYQTALMALFLEE-QCANLTPKHTLLQIHEQVLSKIGSKDSNPAEIELLARDDRDFGKLDAFIKKDVGATLTFSILKYFVSWSPFMNPYLRYVLRDYLTNKNLAQTRGSRNQLTSPPLTSMALSDPSDVPMDEDLPQPIISPS----TGSGLVNQITSWSKSSIGNLAHKGDRPKTPIAIKRCMVKLKDGYPGKDLSQLDKYEIGILMCQIEGLSSEENIEKYKEKLIQLNVTGLVLSVCDMDQLQNEMEMNFGDWQLFKTLIYHLRSVE 2845
Y ALAD+L P PI++GL+++ G+G ++ L KL DE+ + + + ++ S LLF+ + + +++G IVGL T W +VGVS L+++LL+ + + + A L L+L L + F +P +++ KP+ N ++ + + +HM ++++ A+E Y L +RL + C + +++ +CCIPI ++ + ++ +L Y + L+ L+VI + +C L ++ + L S R LK+ + S A T++ E+ + ++ +A+ N + R+ IDA D+ E++ + V LL S N P L+++ D + + + +F E H + + +HLP+Y++ +SG +K M F GG +++ + T G +SV + + + G K ++ ++ LT SD+N + +++L RLL+ IEF L SW++++ WP + +++ L ++ +N +L ++E++ K+ +EL DRD KLDAF++ + L + L+ F+ ++ ++PYLR VL++ ++ + GS P +++ P+ +P P P N++ S + S+ P TP+ I D L+ L + L+ +IE + + + K L + + G VL CDM L++ + ++FG W+LF+ LI LR E
Sbjct: 484 YSSALADVLSEPTLTTPITVGLYAKWGSG-KSFLLNKLRDEMNNFARQWAEPPIRTSGLLFIVCLHVALLIGTIVGLST-WSAVVGVSAAVGFLLLAYLLLAAVRYCNYQMDMQWAYSVQHGLEKRMTRLRLILQVAFCHPPGPQSDSQAKPVRFHFAEANSASPTGDGAVAHMLAALLD----AIESHYGWLATRLYRAFRPKCLKVDVGWRWRRMCCIPIVLIFELALVTVVTGISLTVAYFTFADEKEKEHILVALYVIAAVMGTLICTH--LHVLAKVFVSLFTSHIRVLKRAVRSSE---SAPLTMLGAEVAVMTDMVKCLDAFTNQQS-RLVGVIDALDSCDTERILTLLNAVQTLLS-SPNRPFVLLISVDPHVI----------AKAAEANSRRLFTEGGIGGHDFLRNLVHLPVYLQ--------NSGLRKVQRAQMTALLFKRSGGGDYQTDDGPTLG---HSVSARRLSNASEIISSQEKLRGPARGGGGKKLRLSESVASSTGSNLHRLGQNPQTVLDLSRIVLTDDYFSDVNPRSMRRLMNVIYITVRLLKAFQIEFSWYRLSSWINLTEQWPLRASMIVLHHDQFMDSNADESVSLQSVYEKLRPKLAYLREAAPLLEL----DRDERKLDAFLQLH-KSDLLVADLRIFLPFTINLDPYLRKVLKE---DQQTIEDEGSLVIQARPSVSNTMRQFPAPTTY---VPSPQAYPPYQMFQNEYPANELRSRNLST-------STEPVTPL-INSPSDSFGDDILQTKLTDLTVEGVISLLDRIEDM--KPALPKLAPVLRENAINGRVLKHCDMPDLKSVLGLSFGHWELFRLLITTLRECE 1283
BLAST of Kinase D-interacting substrate vs. Ensembl Fly
Match: CG42672 (gene:FBgn0261554 transcript:FBtr0342885) HSP 1 Score: 132.88 bits (333), Expect = 9.667e-31 Identity = 193/855 (22.57%), Postives = 368/855 (43.04%), Query Frame = 2
Query: 428 YIQALADMLVCPNTNFPISIGLFSRVGAGIRTTHLEKLSDELISLTKINNQKTLQPSLLLFMFPXXXXXXXXXXXXXXTNW--IVGVSLGASLSLMSFLLIEIIIFGSNHKRWGKALRAARWLASFTQSLQLFLDILFRYP--HENELTTKPLSIIITHHNLSNYS---SASHMTLSIVEKLWKAVEKKYSCLLSRL----QISCKNPESSTKFKNICCIPIFVLVIIFFSAIIINATLFTIYNKNPMDIFVNKGLLPLFVIMTCI-VFLCIFGTLPLVCRTITFLVRSPSRQLKQFMESENIDFQASDTLVKNELLKLCQIISIFEAYLNSEKIRMFVFIDAADTESREKLANSIYLVNGLLQMSSNAPIALILAADFQTLNTEWXXXXXXXXXXXXXXXXIFQEISDNFH-LIHSTIHLPIYMEEGIDFNRNSSGKKKYASNGM----FCIRGGNSFESLNMVTTGTKQNSVPVSTRKTSIYXXXXXXXXXXXXXKGASKSIQ---------------------------QLFLTRGDLSDINNKMIKQLTAAAAFIGRLLRINLIEFKLENLISWLSISINWPYQTALMALFLEE-QCANLTPKHTLLQIHEQVLSKIGSKDSNPAEIELLARDDRDFGKLDAFIKKDVGATLTFSILKYFVSWSPFMNPYLRYVLRDYLTNKNLAQTRGSRNQLTSPPLTSMALSDPSDVPMDEDLPQPIISPS----TGSGLVNQITSWSKSSIGNLAHKGDRPKTPIAIKRCMVKLKDGYPGKDLSQLDKYEIGILMCQIEGLSSEENIEKYKEKLIQLNVTGLVLSVCDMDQLQNEMEMNFGDWQLFKTLIYHLRSVE 2845
Y ALAD+L P PI++GL+++ G+G ++ L KL DE+ + + + ++ S LLF+ + + +++G IVGL T W +VGVS L+++LL+ + + + A L L+L L + F +P +++ KP+ N ++ + + +HM ++++ A+E Y L +RL + C + +++ +CCIPI ++ + ++ +L Y + L+ L+VI + +C L ++ + L S R LK+ + S A T++ E+ + ++ +A+ N + R+ IDA D+ E++ + V LL S N P L+++ D + + + +F E H + + +HLP+Y++ +SG +K M F GG +++ + T G +SV + + + G K ++ ++ LT SD+N + +++L RLL+ IEF L SW++++ WP + +++ L ++ +N +L ++E++ K+ +EL DRD KLDAF++ + L + L+ F+ ++ ++PYLR VL++ ++ + GS P +++ P+ +P P P N++ S + S+ P TP+ I D L+ L + L+ +IE + + + K L + + G VL CDM L++ + ++FG W+LF+ LI LR E
Sbjct: 444 YSSALADVLSEPTLTTPITVGLYAKWGSG-KSFLLNKLRDEMNNFARQWAEPPIRTSGLLFIVCLHVALLIGTIVGLST-WSAVVGVSAAVGFLLLAYLLLAAVRYCNYQMDMQWAYSVQHGLEKRMTRLRLILQVAFCHPPGPQSDSQAKPVRFHFAEANSASPTGDGAVAHMLAALLD----AIESHYGWLATRLYRAFRPKCLKVDVGWRWRRMCCIPIVLIFELALVTVVTGISLTVAYFTFADEKEKEHILVALYVIAAVMGTLICTH--LHVLAKVFVSLFTSHIRVLKRAVRSSE---SAPLTMLGAEVAVMTDMVKCLDAFTNQQS-RLVGVIDALDSCDTERILTLLNAVQTLLS-SPNRPFVLLISVDPHVI----------AKAAEANSRRLFTEGGIGGHDFLRNLVHLPVYLQ--------NSGLRKVQRAQMTALLFKRSGGGDYQTDDGPTLG---HSVSARRLSNASEIISSQEKLRGPARGGGGKKLRLSESVASSTGSNLHRLGQNPQTVLDLSRIVLTDDYFSDVNPRSMRRLMNVIYITVRLLKAFQIEFSWYRLSSWINLTEQWPLRASMIVLHHDQFMDSNADESVSLQSVYEKLRPKLAYLREAAPLLEL----DRDERKLDAFLQLH-KSDLLVADLRIFLPFTINLDPYLRKVLKE---DQQTIEDEGSLVIQARPSVSNTMRQFPAPTTY---VPSPQAYPPYQMFQNEYPANELRSRNLST-------STEPVTPL-INSPSDSFGDDILQTKLTDLTVEGVISLLDRIEDM--KPALPKLAPVLRENAINGRVLKHCDMPDLKSVLGLSFGHWELFRLLITTLRECE 1243
BLAST of Kinase D-interacting substrate vs. Ensembl Fly
Match: CG42672 (gene:FBgn0261554 transcript:FBtr0344189) HSP 1 Score: 133.265 bits (334), Expect = 9.838e-31 Identity = 193/855 (22.57%), Postives = 368/855 (43.04%), Query Frame = 2
Query: 428 YIQALADMLVCPNTNFPISIGLFSRVGAGIRTTHLEKLSDELISLTKINNQKTLQPSLLLFMFPXXXXXXXXXXXXXXTNW--IVGVSLGASLSLMSFLLIEIIIFGSNHKRWGKALRAARWLASFTQSLQLFLDILFRYP--HENELTTKPLSIIITHHNLSNYS---SASHMTLSIVEKLWKAVEKKYSCLLSRL----QISCKNPESSTKFKNICCIPIFVLVIIFFSAIIINATLFTIYNKNPMDIFVNKGLLPLFVIMTCI-VFLCIFGTLPLVCRTITFLVRSPSRQLKQFMESENIDFQASDTLVKNELLKLCQIISIFEAYLNSEKIRMFVFIDAADTESREKLANSIYLVNGLLQMSSNAPIALILAADFQTLNTEWXXXXXXXXXXXXXXXXIFQEISDNFH-LIHSTIHLPIYMEEGIDFNRNSSGKKKYASNGM----FCIRGGNSFESLNMVTTGTKQNSVPVSTRKTSIYXXXXXXXXXXXXXKGASKSIQ---------------------------QLFLTRGDLSDINNKMIKQLTAAAAFIGRLLRINLIEFKLENLISWLSISINWPYQTALMALFLEE-QCANLTPKHTLLQIHEQVLSKIGSKDSNPAEIELLARDDRDFGKLDAFIKKDVGATLTFSILKYFVSWSPFMNPYLRYVLRDYLTNKNLAQTRGSRNQLTSPPLTSMALSDPSDVPMDEDLPQPIISPS----TGSGLVNQITSWSKSSIGNLAHKGDRPKTPIAIKRCMVKLKDGYPGKDLSQLDKYEIGILMCQIEGLSSEENIEKYKEKLIQLNVTGLVLSVCDMDQLQNEMEMNFGDWQLFKTLIYHLRSVE 2845
Y ALAD+L P PI++GL+++ G+G ++ L KL DE+ + + + ++ S LLF+ + + +++G IVGL T W +VGVS L+++LL+ + + + A L L+L L + F +P +++ KP+ N ++ + + +HM ++++ A+E Y L +RL + C + +++ +CCIPI ++ + ++ +L Y + L+ L+VI + +C L ++ + L S R LK+ + S A T++ E+ + ++ +A+ N + R+ IDA D+ E++ + V LL S N P L+++ D + + + +F E H + + +HLP+Y++ +SG +K M F GG +++ + T G +SV + + + G K ++ ++ LT SD+N + +++L RLL+ IEF L SW++++ WP + +++ L ++ +N +L ++E++ K+ +EL DRD KLDAF++ + L + L+ F+ ++ ++PYLR VL++ ++ + GS P +++ P+ +P P P N++ S + S+ P TP+ I D L+ L + L+ +IE + + + K L + + G VL CDM L++ + ++FG W+LF+ LI LR E
Sbjct: 608 YSSALADVLSEPTLTTPITVGLYAKWGSG-KSFLLNKLRDEMNNFARQWAEPPIRTSGLLFIVCLHVALLIGTIVGLST-WSAVVGVSAAVGFLLLAYLLLAAVRYCNYQMDMQWAYSVQHGLEKRMTRLRLILQVAFCHPPGPQSDSQAKPVRFHFAEANSASPTGDGAVAHMLAALLD----AIESHYGWLATRLYRAFRPKCLKVDVGWRWRRMCCIPIVLIFELALVTVVTGISLTVAYFTFADEKEKEHILVALYVIAAVMGTLICTH--LHVLAKVFVSLFTSHIRVLKRAVRSSE---SAPLTMLGAEVAVMTDMVKCLDAFTNQQS-RLVGVIDALDSCDTERILTLLNAVQTLLS-SPNRPFVLLISVDPHVI----------AKAAEANSRRLFTEGGIGGHDFLRNLVHLPVYLQ--------NSGLRKVQRAQMTALLFKRSGGGDYQTDDGPTLG---HSVSARRLSNASEIISSQEKLRGPARGGGGKKLRLSESVASSTGSNLHRLGQNPQTVLDLSRIVLTDDYFSDVNPRSMRRLMNVIYITVRLLKAFQIEFSWYRLSSWINLTEQWPLRASMIVLHHDQFMDSNADESVSLQSVYEKLRPKLAYLREAAPLLEL----DRDERKLDAFLQLH-KSDLLVADLRIFLPFTINLDPYLRKVLKE---DQQTIEDEGSLVIQARPSVSNTMRQFPAPTTY---VPSPQAYPPYQMFQNEYPANELRSRNLST-------STEPVTPL-INSPSDSFGDDILQTKLTDLTVEGVISLLDRIEDM--KPALPKLAPVLRENAINGRVLKHCDMPDLKSVLGLSFGHWELFRLLITTLRECE 1407
BLAST of Kinase D-interacting substrate vs. Ensembl Zebrafish
Match: kidins220b (kinase D-interacting substrate 220b [Source:NCBI gene;Acc:335881]) HSP 1 Score: 177.178 bits (448), Expect = 4.648e-44 Identity = 204/840 (24.29%), Postives = 374/840 (44.52%), Query Frame = 2
Query: 428 YIQALADMLVCPNTNFPISIGLFSRVGAGIRTTHLEKLSDELISLTKINNQKTLQPSLLLFMFPXXXXXXXXXXXXXXTNWIVGVSLGASLSLMSFLLIEIIIFGSNHK--RWGKALRAARWLASFTQSLQLFLDILFRYPHE-NELTTK--PLSIIITHHN-LSNYSSASHMTLSIVEKLWKAVEKKYSCLLSRL--QISCKNPESSTKFKNICCIPIFVLVIIFFSAIIINATLFTIYNKNPMDIFVNKGLLPLFVIMTCIVFLCIFGTLPLVCRTITFLVRSP-SRQLKQFMESEN----IDFQASDTLVKNELLKLCQIISIFEAYLNSEKIRMFVFIDAADTESREKLANSIYLVNGLLQMSSNAPIALILAADFQTLNTEWXXXXXXXXXXXXXXXXIFQEISDNFH-LIHSTIHLPIYMEEGIDFNRNSSGKKKYASNGMFCIRGGNSFESLNMVTTGTKQNSVPVSTR---KTSI--YXXXXXXXXXXXXXKGASKSIQQLFLTRGDLSDINNKMIKQLTAAAAFIGRLLRINLIEFKLENLISWLSISINWPYQTALMALFLEEQCANLTPKHTLLQ-IHEQVLSKIGSKDSNPAEIELLARDDRDFGKLDAFIKKDVGATLTFSILKYFVSWSPFMNPYLRYVLRDYLTNKNLAQTRGSRNQLTSPPLTSMALSDPSDVPMDEDLPQPIIS--------------PSTGSGLVNQITSWSKSSIGNLAHKGDRPKTPIAIKRCMVKLKDGYPGKDLSQLDKYEIGILMCQIEGLSSEENIEKYKEKLIQLNVTGLVLSVCDMDQLQNEMEMNFGDWQLFKTLIYHLRSVE 2845
Y ALAD+L P PI +GL+++ G+G ++ L+KL DE+ + + Q S L+ + + L V +++G + + +++ S+ + ++ ++ FGS + W A + LA L+L L ++F P E E TT+ P+ + T +N LS+ + M ++ L A E+++ L +RL ++ + K+K CCIP FV+ + +I+ L ++ + + VN + V M +V L + L CRT + S + Q K+ + N + + ++KNE+ + ++ + + ++ R+ V ID D+ ++K+ + V L S P I A+D + + + +++ ++ + N H + + +HLP+++ +R S KK + GNS + QNS+ T+ KT++ + S ++ S + +L +T SDI+ + +++L + GRLLR N I F + L SW++++ WPY+T+ + L+LEE + P T L+ I+E++ I + ++E L D D + F+ L ++ F+ + ++P LR ++ D R +R Q+ +T L P+ E P + S P G+ Q S S + P+ P R + G P LS + I + I+G+ + I +Y + + N+ G VLS C++D+L+ EM MNFGDWQLF+T + +R VE
Sbjct: 452 YSSALADILSEPTMQPPICVGLYAQWGSG-KSFLLKKLEDEMKTFAGQQVEPLFQFSWLVVLLSLLLCGSVALVLGFTVDPKLAIAISLSILALLYVFFVVVYFGSRREGESWNWAWVISTRLARHIGYLELLLKLMFVNPPELPEQTTRALPVRFLFTDYNRLSSVGGETSMA-EMIATLSDACEREFGFLATRLFRVFKTEDTQGKKKWKKTCCIPSFVIFLFILGCLIMGMALLAVFKVDGQNQTVNA----VLVSMASVVGLALL----LNCRTWWQVTDSVLNSQRKRLHSAANKMHKLKSEGFMKVLKNEVELMAKMAKTIDGFTQNQ-TRLVVIIDGLDSCEQDKVLQMLDTVRVLF---SKGPFISIFASDPHII----------IKAINQNLNSVLRDSNINGHDYMRNIVHLPVFLN-----SRGLSSAKKMCAPAPANGETGNSEGWHEELDRKLSQNSLGDQTKFGSKTTLNRRDTYRRRQMQRSVTRQMSFDLTKLLVTEDWFSDISPQTMRRLLNIVSVTGRLLRANQISFNWDRLASWINLTEQWPYRTSWLILYLEE--TDGIPDQTNLKTIYERISKNIPTT----KDVEPLLEIDGDVRSFEVFLSSRT-PVLAARDIRTFLPCTVNLDPKLREIIAD---------VRAAREQVNMAGVTYPTL------PLQEGRPISMYSQQSSACSPTASFNGPYNPPGVSPQPHSAYFSGMAG-------PQHPF-YNRGSASVVSGTPSILLSSMSTDVICERVKLIDGID-QNLISQYTATIKKANINGRVLSQCNIDELKKEMNMNFGDWQLFRTSVLEMRHVE 1231
BLAST of Kinase D-interacting substrate vs. Ensembl Zebrafish
Match: kidins220a (kinase D-interacting substrate 220a [Source:ZFIN;Acc:ZDB-GENE-041014-170]) HSP 1 Score: 170.629 bits (431), Expect = 4.946e-42 Identity = 210/850 (24.71%), Postives = 375/850 (44.12%), Query Frame = 2
Query: 428 YIQALADMLVCPNTNFPISIGLFSRVGAGIRTTHLEKLSDELISLTKINNQKTLQPSLLLFMFPXXXXXXXXXXXXXXTNWIVGVSLGASLSLMSFLLIEIIIFGSNH--KRWGKALRAARWLASFTQSLQLFLDILFRYPHE-NELTTK--PLSIIITHHN-LSNYSSASHMTLSIVEKLWKAVEKKYSCLLSRLQISCKNPES-STKFKNICCIPIFVLVIIFFSAIIINATLFTIYNKNPMDIFVNKGLLPLFVIMTCIVFLCIFGTLPLVCRTITFLVRSP-SRQLKQFMESEN----IDFQASDTLVKNELLKLCQIISIFEAYLNSEKIRMFVFIDAADTESREKLANSIYLVNGLLQMSSNAPIALILAADFQTLNTEWXXXXXXXXXXXXXXXXIFQEISDNFH-LIHSTIHLPIYMEEGIDFNRNSSGKKKYASNGMFCIR-GGNSFESLNM------VTTGTKQNSVPVSTRKTSIYX-----XXXXXXXXXXXXKGASKSIQQLFLTRGDLSDINNKMIKQLTAAAAFIGRLLRINLIEFKLENLISWLSISINWPYQTALMALFLEEQCANLTPKHTLLQIHEQVLSKIGSKDSNPAEIELLARDDRDFGKLDAFIKKDVGATLTFSILKYFVSWSPFMNPYLRYVLRDYLTNKNLAQTRGSRNQ--LTSPPLTSMALSDPSDVPMDEDLPQPIISPSTGSGLVNQITSWSKSSIGNLAHKGDRPKT---------------PIAIKRCMVKLKDGYPGKD--LSQLDKYEIGILMCQIEGLSSEENIEKYKEKLIQLNVTGLVLSVCDMDQLQNEMEMNFGDWQLFKTLIYHLRSVE 2845
Y ALAD+L P PI +GL+++ G+G ++ L+KL DE+ + + Q S L+ + + L V +++G + + +++ +L + ++ ++ FG + W A + LA L+L L ++F P E E TTK P+ + T +N LS+ + M ++ L A E+++ L +RL KN E+ K+K CC+P FVL ++ +I L I+ + ++ VN + + M +V L + L CRT + S Q ++ + + N + + ++K E+ + ++ +++ ++ R+ V ID D+ ++K+ + V L S P I A+D + + + +++ ++ + N H + + IHLP+++ NS G ++ CI N + LN V QNS+ R+ S + S ++ S + +L +T SDI+ + +++L + GRLLR N I F + L SW++++ WPY+T+ + LFLEE +T TL I+E++ I + ++E L D D + F+ LT ++ F+ + ++P LR ++ D R +R Q L ++ L D S + P +G G + S + S G+ + G P T P + +V G LS L + + QI+GL S +E+Y + + NV G VLS C++D+L+ EM MNFGDW LF++ + R E
Sbjct: 451 YSSALADILSEPTMQPPICVGLYAQWGSG-KSFLLKKLEDEMKTFAGQQVEPLFQFSWLVVVLTLLLCGSVALVLGFAVDTKLAIAVALTLLALMYIFFVLVYFGGRKAGESWSWAWVLSTRLARHIGYLELLLKLMFVNPPELPEQTTKALPVRFLFTDYNRLSSVGGETSMA-EMIATLSDACEREFGFLATRLFRVFKNDEAQGKKWKKTCCVPSFVLFVLTSGCLITGMALLAIFKVDSENLTVNA----VLISMASVVGLALL----LNCRTWWQVADSVLHSQRRRLLNAANRMHKLKSEGFMKVLKCEVELMAKMAKTIDSFTQNQ-TRLAVIIDGLDSCEQDKVLQMLDTVRVLF---SKGPFISIFASDPHII----------IKAINQNLNSVLRDSNINGHDYMRNIIHLPVFL--------NSRG---LSTAKRLCISTAANGADQLNNDAWQEDVDRKLSQNSLGDQGRQGSKTALNRRDTYRRRQMQRSVTRQMSFDLTKLLVTEDWFSDISPQTMRRLLNIVSVTGRLLRANQIIFNWDRLASWINLTEEWPYRTSWIILFLEE-TDGVTDLVTLKTIYERISKNIPTT----KDVEPLLEIDGDIRSFEVFLSSHT-PVLTARDVEMFLPCTVNLDPKLREIIAD---------VRDAREQMHLGGVSFPTLPLQDAS------------VRPHSGFGQQSLACSPTGSFPGSFPNAGVLPATQPHSSYFSGLTGPQHPFYNRGSVVS-----SGSQVLLSSLTIDGVCEKLRQIQGLDS-SMLEQYIGTIKKANVNGRVLSQCNLDELKKEMNMNFGDWHLFRSTVVEQRHAE 1232
BLAST of Kinase D-interacting substrate vs. Ensembl Zebrafish
Match: kidins220a (kinase D-interacting substrate 220a [Source:ZFIN;Acc:ZDB-GENE-041014-170]) HSP 1 Score: 167.162 bits (422), Expect = 5.194e-41 Identity = 212/875 (24.23%), Postives = 378/875 (43.20%), Query Frame = 2
Query: 428 YIQALADMLVCPNTNFPISIGLFSRVGAGIRTTHLEKLSDELISLTKINNQKTLQPSLLLFMFPXXXXXXXXXXXXXXTNWIVGVSLGASLSLMSFLLIEIIIFGSNH--KRWGKALRAARWLASFTQSLQLFLDILFRYPHE-NELTTK--PLSIIITHHN-LSNYSSASHMTLSIVEKLWKAVEKKYSCLLSRLQISCKNPES-STKFKNICCIPIFVLVIIFFSAIIINATLFTIYNKNPMDIFVNKGLLPLFVIMTCIVFLCIFGTLPLVCRTITFLVRSP-SRQLKQFMESEN----IDFQASDTLVKNELLKLCQIISIFEAYLNSEKIRMFVFIDAADTESREKLANSIYLVNGLLQMSSNAPIALILAADFQTLNTEWXXXXXXXXXXXXXXXXIFQEISDNFH-LIHSTIHLPIYMEEGIDFNRNSSGKKKYASNGMFCIR-GGNSFESLNM------VTTGTKQNSVPVSTRKTSIYX-----XXXXXXXXXXXXKGASKSIQQLFLTRGDLSDINNKMIKQLTAAAAFIGRLLRINLIEFKLENLISWLSISINWPYQTALMALFLEEQCANLTPKHTLLQIHEQVLSKIGSKDSNPAEIELLARDDRDFGKLDAFIKKDVGATLTFSILKYFVSWSPFMNPYLRYVLRDYLTNKNLAQTRG-------------------SRNQLTSPPLTSMALSDPSDVPMDEDLPQPIISPSTGSGLV-------------NQITSWSKSSIGNLAHKGDRPKTPIA-------IKRCMVKLKDGYPGKDLSQLDKYEIGI-LMC----QIEGLSSEENIEKYKEKLIQLNVTGLVLSVCDMDQLQNEMEMNFGDWQLFKTLIYHLRSVE 2845
Y ALAD+L P PI +GL+++ G+G ++ L+KL DE+ + + Q S L+ + + L V +++G + + +++ +L + ++ ++ FG + W A + LA L+L L ++F P E E TTK P+ + T +N LS+ + M ++ L A E+++ L +RL KN E+ K+K CC+P FVL ++ +I L I+ + ++ VN + + M +V L + L CRT + S Q ++ + + N + + ++K E+ + ++ +++ ++ R+ V ID D+ ++K+ + V L S P I A+D + + + +++ ++ + N H + + IHLP+++ NS G ++ CI N + LN V QNS+ R+ S + S ++ S + +L +T SDI+ + +++L + GRLLR N I F + L SW++++ WPY+T+ + LFLEE +T TL I+E++ I + ++E L D D + F+ LT ++ F+ + ++P LR ++ D + G + L P S P P LP S SGL + + ++ G+ H RP + + IK ++K G SQ+ + I +C QI+GL S +E+Y + + NV G VLS C++D+L+ EM MNFGDW LF++ + R E
Sbjct: 451 YSSALADILSEPTMQPPICVGLYAQWGSG-KSFLLKKLEDEMKTFAGQQVEPLFQFSWLVVVLTLLLCGSVALVLGFAVDTKLAIAVALTLLALMYIFFVLVYFGGRKAGESWSWAWVLSTRLARHIGYLELLLKLMFVNPPELPEQTTKALPVRFLFTDYNRLSSVGGETSMA-EMIATLSDACEREFGFLATRLFRVFKNDEAQGKKWKKTCCVPSFVLFVLTSGCLITGMALLAIFKVDSENLTVNA----VLISMASVVGLALL----LNCRTWWQVADSVLHSQRRRLLNAANRMHKLKSEGFMKVLKCEVELMAKMAKTIDSFTQNQ-TRLAVIIDGLDSCEQDKVLQMLDTVRVLF---SKGPFISIFASDPHII----------IKAINQNLNSVLRDSNINGHDYMRNIIHLPVFL--------NSRG---LSTAKRLCISTAANGADQLNNDAWQEDVDRKLSQNSLGDQGRQGSKTALNRRDTYRRRQMQRSVTRQMSFDLTKLLVTEDWFSDISPQTMRRLLNIVSVTGRLLRANQIIFNWDRLASWINLTEEWPYRTSWIILFLEE-TDGVTDLVTLKTIYERISKNIPTT----KDVEPLLEIDGDIRSFEVFLSSHT-PVLTARDVEMFLPCTVNLDPKLREIIADVRDAREQMHLGGVSFPTLPLQDASVRPHSGFGQQSLACSPTGSF----PGSFPNAGVLPATQPHSSYFSGLTGPQHPFYNRPYFPHHVYHLPRNYPGHPHHAPTRPASKLHKDPGLGLIKSQPFRMKQGSVVSSGSQVLLSSLTIDGVCEKLRQIQGLDS-SMLEQYIGTIKKANVNGRVLSQCNLDELKKEMNMNFGDWHLFRSTVVEQRHAE 1279
BLAST of Kinase D-interacting substrate vs. Ensembl Xenopus
Match: rab4b (RAB4B member RAS oncogene family [Source:Xenbase;Acc:XB-GENE-946075]) HSP 1 Score: 185.652 bits (470), Expect = 1.651e-46 Identity = 207/894 (23.15%), Postives = 400/894 (44.74%), Query Frame = 2
Query: 428 YIQALADMLVCPNTNFPISIGLFSRVGAGIRTTHLEKLSDELISLTKINNQKTLQPSLLLFMFPXXXXXXXXXXXXXXTNWIVGVSLGASLSLMSFLLIEIIIFGSNHK--RWGKALRAARWLASFTQSLQLFLDILFRYPHE-NELTTK--PLSIIITHHN-LSNYSSASHMTLSIVEKLWKAVEKKYSCLLSRLQISCKNPESS--TKFKNICCIPIFVLVIIFFSAIIINATLFTIYNKNPMDIFVNKGLLPLFVIMTCIVFLCIFGTLPLVCRT----ITFLVRSPSRQLKQFMES-ENIDFQASDTLVKNELLKLCQIISIFEAYLNSEKIRMFVFIDAADTESREKLANSIYLVNGLLQMSSNAPIALILAADFQTLNTEWXXXXXXXXXXXXXXXXIFQEISDNFH-LIHSTIHLPIYMEEGIDFNRNSSGKKKY----ASNG-MFCIRGGNSFESLNMVTTGTKQNSVPVSTR---KTSI--YXXXXXXXXXXXXXKGASKSIQQLFLTRGDLSDINNKMIKQLTAAAAFIGRLLRINLIEFKLENLISWLSISINWPYQTALMALFLEEQCANLTPKHTLLQIHEQVLSKIGSKDSNPAEIELLARDDRDFGKLDAFIKKDVGATLTFSILKYFVSWSPFMNPYLRYVLRDYLTNKNLAQTRGSRNQLT--SPPLTSMALSDP---------------SDVPMDEDLPQPIISPSTGSGLVNQITS----------------WSKSSIGNLAHKGDRP--KTPIA-------------IKRCMVKLKDGYPGKDLSQLDKYEIGILMCQIEGLSSEEN--IEKYKEKLIQLNVTGLVLSVCDMDQLQNEMEMNFGDWQLFKTLIYHLRSVEA-FKSDDLSIVGDP 2884
Y ALAD+L P PI +GL+++ G+G ++ L+KL DE+ + + Q S L+ + L +G+++ + +G+S+ SL + ++ + FG + W A + LA L+L L ++F P E E TTK P+ + T +N LS+ + M ++ L A E+++ L +RL K E+ K+K CC+P FV+ I I L I+ +P ++ VN + + + C+V L L CRT + ++ S ++L + + ++K E+ + ++ +++ ++ R+ V ID D ++K+ +++++ + + S P I A+D + + + +++ ++ + N H + + +HLP+++ +R S KK A+NG + C+ G+ E L+ + Q+S+ +++ KT++ + + ++ S + +L +T SDI+ + +++L + GRLLR N I F + L SW++++ WPY+T+ + L+LEE + + TL I+E++ I + ++E L D D + F+ + + K F+ + ++P LR ++ D R +R+Q+ P ++ L D S + P ++SP S + +T + GN H RP K ++ ++R V L+ G G S L + + +++G+ E + +Y + + N+ G VL+ C MD+L+ EM MNFGDW LFK+ ++ +RS + + ++ +VG+P
Sbjct: 444 YSSALADILSEPTMQPPICVGLYAQWGSG-KSFLLKKLEDEMKTFAGQQIEPLFQFSWLIVFLILMLCSTIGLLLSFTVDPKLGISVALSLLTVLYIFFAAVYFGGRREGENWNWAWVLSTRLARQIGYLELLLKLMFVNPPELPEQTTKALPVRFLFTDYNRLSSVGGETSMA-EMIATLSDACEREFGFLATRLFRVFKTEETQGKKKWKKTCCLPSFVIFIFIMLCIFAGVLLLAIFKVDPKNMTVNA----ILIAVACVVGLVFV----LNCRTWWQVMDSILNSQRKRLHGAASKLHKLKSEGFMKVLKWEVELMAKMAKTIDSFTQNQ-TRLVVIIDGLDACEQDKV---LHMLDTVRILFSKGPFIAIFASDPHII----------IKAINQNLNSVLRDSNINGHDYMRNIVHLPVFLN-----SRGLSNAKKLCSISATNGDVGCMERGH--EDLDRRVS---QHSLAEASKLGSKTALNRRDTYRRRQMQRTITRQMSFDLTKLLVTEDWFSDISPQTMRRLLNIVSVTGRLLRANQISFHWDRLASWINLTEQWPYRTSWLILYLEE-TEGVPDQSTLKTIYERISKNIPTT----KDVEPLLEIDSDIRSFEVFLASRTPVLIARDV-KTFLPCTVNLDPKLREIIAD---------VRAARDQMNIGGFPYPTLPLQDAPPRATSVFSQPSSVCSSSSFNGPFPAGVMSPQPPSSYYSGMTGPQHPFYNRPFFAPHVYLPRYYFGNPQHPMSRPSFKHSVSRDQNNGLVSIVTNVQRATVSLQAGETGAHSSALAAMNVDGVCEKLKGIDGIEQSMLPQYTATIRKANINGRVLTQCTMDELKKEMNMNFGDWHLFKSALHEMRSSDTQIQPEEGRMVGEP 1288
BLAST of Kinase D-interacting substrate vs. Ensembl Xenopus
Match: rab4b (RAB4B member RAS oncogene family [Source:Xenbase;Acc:XB-GENE-946075]) HSP 1 Score: 182.185 bits (461), Expect = 2.133e-45 Identity = 203/883 (22.99%), Postives = 394/883 (44.62%), Query Frame = 2
Query: 428 YIQALADMLVCPNTNFPISIGLFSRVGAGIRTTHLEKLSDELISLTKINNQKTLQPSLLLFMFPXXXXXXXXXXXXXXTNWIVGVSLGASLSLMSFLLIEIIIFGSNHK--RWGKALRAARWLASFTQSLQLFLDILFRYPHE-NELTTK--PLSIIITHHN-LSNYSSASHMTLSIVEKLWKAVEKKYSCLLSRLQISCKNPESS--TKFKNICCIPIFVLVIIFFSAIIINATLFTIYNKNPMDIFVNKGLLPLFVIMTCIVFLCIFGTLPLVCRT----ITFLVRSPSRQLKQFMES-ENIDFQASDTLVKNELLKLCQIISIFEAYLNSEKIRMFVFIDAADTESREKLANSIYLVNGLLQMSSNAPIALILAADFQTLNTEWXXXXXXXXXXXXXXXXIFQEISDNFH-LIHSTIHLPIYMEEGIDFNRNSSGKKKY----ASNG-MFCIRGGNS-FESLNMVTTGTKQNSVPVSTR---KTSI--YXXXXXXXXXXXXXKGASKSIQQLFLTRGDLSDINNKMIKQLTAAAAFIGRLLRINLIEFKLENLISWLSISINWPYQTALMALFLEEQCANLTPKHTLLQIHEQVLSKIGSKDSNPAEIELLARDDRDFGKLDAFIKKDVGATLTFSILKYFVSWSPFMNPYLRYVLRDYLTNKNLAQTRGSRNQLT--SPPLTSMALSDP---------------SDVPMDEDLPQPIISPSTGSGLVNQITS----------------WSKSSIGNLAHKGDRPKTPIAIKRCM---VKLKDGYPGKDLSQLDKYEIGILMCQIEGLSSEEN--IEKYKEKLIQLNVTGLVLSVCDMDQLQNEMEMNFGDWQLFKTLIYHLRSVEA-FKSDDLSIVGDP 2884
Y ALAD+L P PI +GL+++ G+G ++ L+KL DE+ + + Q S L+ + L +G+++ + +G+S+ SL + ++ + FG + W A + LA L+L L ++F P E E TTK P+ + T +N LS+ + M ++ L A E+++ L +RL K E+ K+K CC+P FV+ I I L I+ +P ++ VN + + + C+V L L CRT + ++ S ++L + + ++K E+ + ++ +++ ++ R+ V ID D ++K+ +++++ + + S P I A+D + + + +++ ++ + N H + + +HLP+++ +R S KK A+NG + C+ G+ E L+ + Q+S+ +++ KT++ + + ++ S + +L +T SDI+ + +++L + GRLLR N I F + L SW++++ WPY+T+ + L+LEE + + TL I+E++ I + ++E L D D + F+ + + K F+ + ++P LR ++ D R +R+Q+ P ++ L D S + P ++SP S + +T + GN H RP ++ R + G S L + + +++G+ E + +Y + + N+ G VL+ C MD+L+ EM MNFGDW LFK+ ++ +RS + + ++ +VG+P
Sbjct: 444 YSSALADILSEPTMQPPICVGLYAQWGSG-KSFLLKKLEDEMKTFAGQQIEPLFQFSWLIVFLILMLCSTIGLLLSFTVDPKLGISVALSLLTVLYIFFAAVYFGGRREGENWNWAWVLSTRLARQIGYLELLLKLMFVNPPELPEQTTKALPVRFLFTDYNRLSSVGGETSMA-EMIATLSDACEREFGFLATRLFRVFKTEETQGKKKWKKTCCLPSFVIFIFIMLCIFAGVLLLAIFKVDPKNMTVNA----ILIAVACVVGLVFV----LNCRTWWQVMDSILNSQRKRLHGAASKLHKLKSEGFMKVLKWEVELMAKMAKTIDSFTQNQ-TRLVVIIDGLDACEQDKV---LHMLDTVRILFSKGPFIAIFASDPHII----------IKAINQNLNSVLRDSNINGHDYMRNIVHLPVFLN-----SRGLSNAKKLCSISATNGDVGCMESGSGGHEDLDRRVS---QHSLAEASKLGSKTALNRRDTYRRRQMQRTITRQMSFDLTKLLVTEDWFSDISPQTMRRLLNIVSVTGRLLRANQISFHWDRLASWINLTEQWPYRTSWLILYLEE-TEGVPDQSTLKTIYERISKNIPTT----KDVEPLLEIDSDIRSFEVFLASRTPVLIARDV-KTFLPCTVNLDPKLREIIAD---------VRAARDQMNIGGFPYPTLPLQDAPPRATSVFSQPSSVCSSSSFNGPFPAGVMSPQPPSSYYSGMTGPQHPFYNRPFFAPHVYLPRYYFGNPQHPMSRPSFKHSVSRDQNNGLATSSSVAGAHSSALAAMNVDGVCEKLKGIDGIEQSMLPQYTATIRKANINGRVLTQCTMDELKKEMNMNFGDWHLFKSALHEMRSSDTQIQPEEGRMVGEP 1279
BLAST of Kinase D-interacting substrate vs. Ensembl Xenopus
Match: rab4b (RAB4B member RAS oncogene family [Source:Xenbase;Acc:XB-GENE-946075]) HSP 1 Score: 181.03 bits (458), Expect = 4.328e-45 Identity = 202/881 (22.93%), Postives = 389/881 (44.15%), Query Frame = 2
Query: 428 YIQALADMLVCPNTNFPISIGLFSRVGAGIRTTHLEKLSDELISLTKINNQKTLQPSLLLFMFPXXXXXXXXXXXXXXTNWIVGVSLGASLSLMSFLLIEIIIFGSNHK--RWGKALRAARWLASFTQSLQLFLDILFRYPHE-NELTTK--PLSIIITHHN-LSNYSSASHMTLSIVEKLWKAVEKKYSCLLSRLQISCKNPESS--TKFKNICCIPIFVLVIIFFSAIIINATLFTIYNKNPMDIFVNKGLLPLFVIMTCIVFLCIFGTLPLVCRT----ITFLVRSPSRQLKQFMES-ENIDFQASDTLVKNELLKLCQIISIFEAYLNSEKIRMFVFIDAADTESREKLANSIYLVNGLLQMSSNAPIALILAADFQTLNTEWXXXXXXXXXXXXXXXXIFQEISDNFH-LIHSTIHLPIYMEEGIDFNRNSSGKKKY----ASNG-MFCIRGGNS-FESLNMVTTGTKQNSVPVSTRKTSIYXXXXXXXXXXXXXKGASKSIQQLFLTRGDLSDINNKMIKQLTAAAAFIGRLLRINLIEFKLENLISWLSISINWPYQTALMALFLEEQCANLTPKHTLLQIHEQVLSKIGSKDSNPAEIELLARDDRDFGKLDAFIKKDVGATLTFSILKYFVSWSPFMNPYLRYVLRDYLTNKNLAQTRGSRNQLT--SPPLTSMALSDP---------------SDVPMDEDLPQPIISPSTGSGLVNQITS----------------WSKSSIGNLAHKGDRPKTPIAIKR----CMVKLKDGYP--GKDLSQLDKYEIGILMCQIEGLSSEEN--IEKYKEKLIQLNVTGLVLSVCDMDQLQNEMEMNFGDWQLFKTLIYHLRSVEA-FKSDDLSIVGDP 2884
Y ALAD+L P PI +GL+++ G+G ++ L+KL DE+ + + Q S L+ + L +G+++ + +G+S+ SL + ++ + FG + W A + LA L+L L ++F P E E TTK P+ + T +N LS+ + M ++ L A E+++ L +RL K E+ K+K CC+P FV+ I I L I+ +P ++ VN + + + C+V L L CRT + ++ S ++L + + ++K E+ + ++ +++ ++ R+ V ID D ++K+ +++++ + + S P I A+D + + + +++ ++ + N H + + +HLP+++ +R S KK A+NG + C+ G+ E L+ + + R+ + + ++ S + +L +T SDI+ + +++L + GRLLR N I F + L SW++++ WPY+T+ + L+LEE + + TL I+E++ I + ++E L D D + F+ + + K F+ + ++P LR ++ D R +R+Q+ P ++ L D S + P ++SP S + +T + GN H RP ++ R +V + P G S L + + +++G+ E + +Y + + N+ G VL+ C MD+L+ EM MNFGDW LFK+ ++ +RS + + ++ +VG+P
Sbjct: 444 YSSALADILSEPTMQPPICVGLYAQWGSG-KSFLLKKLEDEMKTFAGQQIEPLFQFSWLIVFLILMLCSTIGLLLSFTVDPKLGISVALSLLTVLYIFFAAVYFGGRREGENWNWAWVLSTRLARQIGYLELLLKLMFVNPPELPEQTTKALPVRFLFTDYNRLSSVGGETSMA-EMIATLSDACEREFGFLATRLFRVFKTEETQGKKKWKKTCCLPSFVIFIFIMLCIFAGVLLLAIFKVDPKNMTVNA----ILIAVACVVGLVFV----LNCRTWWQVMDSILNSQRKRLHGAASKLHKLKSEGFMKVLKWEVELMAKMAKTIDSFTQNQ-TRLVVIIDGLDACEQDKV---LHMLDTVRILFSKGPFIAIFASDPHII----------IKAINQNLNSVLRDSNINGHDYMRNIVHLPVFLN-----SRGLSNAKKLCSISATNGDVGCMESGSGGHEDLDRRVSHKSILCTCDTYRRRQM---------QRTITRQMSFDLTKLLVTEDWFSDISPQTMRRLLNIVSVTGRLLRANQISFHWDRLASWINLTEQWPYRTSWLILYLEE-TEGVPDQSTLKTIYERISKNIPTT----KDVEPLLEIDSDIRSFEVFLASRTPVLIARDV-KTFLPCTVNLDPKLREIIAD---------VRAARDQMNIGGFPYPTLPLQDAPPRATSVFSQPSSVCSSSSFNGPFPAGVMSPQPPSSYYSGMTGPQHPFYNRPFFAPHVYLPRYYFGNPQHPMSRPSFKHSVSRDQNNGLVSIVTNVPTAGAHSSALAAMNVDGVCEKLKGIDGIEQSMLPQYTATIRKANINGRVLTQCTMDELKKEMNMNFGDWHLFKSALHEMRSSDTQIQPEEGRMVGEP 1271
BLAST of Kinase D-interacting substrate vs. Ensembl Xenopus
Match: rab4b (RAB4B member RAS oncogene family [Source:Xenbase;Acc:XB-GENE-946075]) HSP 1 Score: 179.489 bits (454), Expect = 1.333e-44 Identity = 196/856 (22.90%), Postives = 380/856 (44.39%), Query Frame = 2
Query: 428 YIQALADMLVCPNTNFPISIGLFSRVGAGIRTTHLEKLSDELISLTKINNQKTLQPSLLLFMFPXXXXXXXXXXXXXXTNWIVGVSLGASLSLMSFLLIEIIIFGSNHK--RWGKALRAARWLASFTQSLQLFLDILFRYPHE-NELTTK--PLSIIITHHN-LSNYSSASHMTLSIVEKLWKAVEKKYSCLLSRLQISCKNPESS--TKFKNICCIPIFVLVIIFFSAIIINATLFTIYNKNPMDIFVNKGLLPLFVIMTCIVFLCIFGTLPLVCRT----ITFLVRSPSRQLKQFMES-ENIDFQASDTLVKNELLKLCQIISIFEAYLNSEKIRMFVFIDAADTESREKLANSIYLVNGLLQMSSNAPIALILAADFQTLNTEWXXXXXXXXXXXXXXXXIFQEISDNFH-LIHSTIHLPIYMEEGIDFNRNSSGKKKY----ASNG-MFCIRGGNSFESLNMVTTGTKQNSVPVSTRKTSIYXXXXXXXXXXXXXKGASKSIQQLFLTRGDLSDINNKMIKQLTAAAAFIGRLLRINLIEFKLENLISWLSISINWPYQTALMALFLEEQCANLTPKHTLLQIHEQVLSKIGSKDSNPAEIELLARDDRDFGKLDAFIKKDVGATLTFSILKYFVSWSPFMNPYLRYVLRDYLTNKNLAQTRGSRNQLT--SPPLTSMALSDP---------------SDVPMDEDLPQPIISPSTGSGLVNQITSWSKSSIGNLAHKGDRPKTPIAIKRCMVKLKDGYPGKDLSQLDKYEIGILMCQIEGLSSEENIEKYKEKLIQLNVTGLVLSVCDMDQLQNEMEMNFGDWQLFKTLIYHLRSVEA-FKSDDLSIVGDP 2884
Y ALAD+L P PI +GL+++ G+G ++ L+KL DE+ + + Q S L+ + L +G+++ + +G+S+ SL + ++ + FG + W A + LA L+L L ++F P E E TTK P+ + T +N LS+ + M ++ L A E+++ L +RL K E+ K+K CC+P FV+ I I L I+ +P ++ VN + + + C+V L L CRT + ++ S ++L + + ++K E+ + ++ +++ ++ R+ V ID D ++K+ +++++ + + S P I A+D + + + +++ ++ + N H + + +HLP+++ +R S KK A+NG + C+ G + V+ + + R+ + ++ S + +L +T SDI+ + +++L + GRLLR N I F + L SW++++ WPY+T+ + L+LEE + + TL I+E++ I + ++E L D D + F+ + + K F+ + ++P LR ++ D R +R+Q+ P ++ L D S + P ++SP S + +T P+ P R L G L+ ++ + + I+G+ + + +Y + + N+ G VL+ C MD+L+ EM MNFGDW LFK+ ++ +RS + + ++ +VG+P
Sbjct: 411 YSSALADILSEPTMQPPICVGLYAQWGSG-KSFLLKKLEDEMKTFAGQQIEPLFQFSWLIVFLILMLCSTIGLLLSFTVDPKLGISVALSLLTVLYIFFAAVYFGGRREGENWNWAWVLSTRLARQIGYLELLLKLMFVNPPELPEQTTKALPVRFLFTDYNRLSSVGGETSMA-EMIATLSDACEREFGFLATRLFRVFKTEETQGKKKWKKTCCLPSFVIFIFIMLCIFAGVLLLAIFKVDPKNMTVNA----ILIAVACVVGLVFV----LNCRTWWQVMDSILNSQRKRLHGAASKLHKLKSEGFMKVLKWEVELMAKMAKTIDSFTQNQ-TRLVVIIDGLDACEQDKV---LHMLDTVRILFSKGPFIAIFASDPHII----------IKAINQNLNSVLRDSNINGHDYMRNIVHLPVFLN-----SRGLSNAKKLCSISATNGDVGCMESGGHEDLDRRVSHKSILCTCDTYRRR----------QMQRTITRQMSFDLTKLLVTEDWFSDISPQTMRRLLNIVSVTGRLLRANQISFHWDRLASWINLTEQWPYRTSWLILYLEE-TEGVPDQSTLKTIYERISKNIPTT----KDVEPLLEIDSDIRSFEVFLASRTPVLIARDV-KTFLPCTVNLDPKLREIIAD---------VRAARDQMNIGGFPYPTLPLQDAPPRATSVFSQPSSVCSSSSFNGPFPAGVMSPQPPSSYYSGMTG---------------PQHPF-YNRVGAVLVAGAHSSALAAMNVDGVCEKLKGIDGI-EQSMLPQYTATIRKANINGRVLTQCTMDELKKEMNMNFGDWHLFKSALHEMRSSDTQIQPEEGRMVGEP 1195
BLAST of Kinase D-interacting substrate vs. Ensembl Xenopus
Match: rab4b (RAB4B member RAS oncogene family [Source:Xenbase;Acc:XB-GENE-946075]) HSP 1 Score: 179.104 bits (453), Expect = 1.779e-44 Identity = 199/864 (23.03%), Postives = 389/864 (45.02%), Query Frame = 2
Query: 428 YIQALADMLVCPNTNFPISIGLFSRVGAGIRTTHLEKLSDELISLTKINNQKTLQPSLLLFMFPXXXXXXXXXXXXXXTNWIVGVSLGASLSLMSFLLIEIIIFGSNHK--RWGKALRAARWLASFTQSLQLFLDILFRYPHE-NELTTK--PLSIIITHHN-LSNYSSASHMTLSIVEKLWKAVEKKYSCLLSRLQISCKNPESS--TKFKNICCIPIFVLVIIFFSAIIINATLFTIYNKNPMDIFVNKGLLPLFVIMTCIVFLCIFGTLPLVCRT----ITFLVRSPSRQLKQFMES-ENIDFQASDTLVKNELLKLCQIISIFEAYLNSEKIRMFVFIDAADTESREKLANSIYLVNGLLQMSSNAPIALILAADFQTLNTEWXXXXXXXXXXXXXXXXIFQEISDNFH-LIHSTIHLPIYMEEGIDFNRNSSGKKKY----ASNG-MFCIRGGNS-FESLNMVTTGTKQNSVPVSTR---KTSI--YXXXXXXXXXXXXXKGASKSIQQLFLTRGDLSDINNKMIKQLTAAAAFIGRLLRINLIEFKLENLISWLSISINWPYQTALMALFLEEQCANLTPKHTLLQIHEQVLSKIGSKDSNPAEIELLARDDRDFGKLDAFIKKDVGATLTFSILKYFVSWSPFMNPYLRYVLRDYLTNKNLAQTRGSRNQLT--SPPLTSMALSDP---------------SDVPMDEDLPQPIISPSTGSGLVNQITSWSKSSIGNLAHKGDRPKTPIAIKRCMVKLKDGYPGKDLSQLDKYEIGILMCQIEGLSSEEN--IEKYKEKLIQLNVTGLVLSVCDMDQLQNEMEMNFGDWQLFKTLIYHLRSVEA-FKSDDLSIVGDP 2884
Y ALAD+L P PI +GL+++ G+G ++ L+KL DE+ + + Q S L+ + L +G+++ + +G+S+ SL + ++ + FG + W A + LA L+L L ++F P E E TTK P+ + T +N LS+ + M ++ L A E+++ L +RL K E+ K+K CC+P FV+ I I L I+ +P ++ VN + + + C+V L L CRT + ++ S ++L + + ++K E+ + ++ +++ ++ R+ V ID D ++K+ +++++ + + S P I A+D + + + +++ ++ + N H + + +HLP+++ +R S KK A+NG + C+ G+ E L+ + Q+S+ +++ KT++ + + ++ S + +L +T SDI+ + +++L + GRLLR N I F + L SW++++ WPY+T+ + L+LEE + + TL I+E++ I + ++E L D D + F+ + + K F+ + ++P LR ++ D R +R+Q+ P ++ L D S + P ++SP S + +T P+ P + + G S L + + +++G+ E + +Y + + N+ G VL+ C MD+L+ EM MNFGDW LFK+ ++ +RS + + ++ +VG+P
Sbjct: 444 YSSALADILSEPTMQPPICVGLYAQWGSG-KSFLLKKLEDEMKTFAGQQIEPLFQFSWLIVFLILMLCSTIGLLLSFTVDPKLGISVALSLLTVLYIFFAAVYFGGRREGENWNWAWVLSTRLARQIGYLELLLKLMFVNPPELPEQTTKALPVRFLFTDYNRLSSVGGETSMA-EMIATLSDACEREFGFLATRLFRVFKTEETQGKKKWKKTCCLPSFVIFIFIMLCIFAGVLLLAIFKVDPKNMTVNA----ILIAVACVVGLVFV----LNCRTWWQVMDSILNSQRKRLHGAASKLHKLKSEGFMKVLKWEVELMAKMAKTIDSFTQNQ-TRLVVIIDGLDACEQDKV---LHMLDTVRILFSKGPFIAIFASDPHII----------IKAINQNLNSVLRDSNINGHDYMRNIVHLPVFLN-----SRGLSNAKKLCSISATNGDVGCMESGSGGHEDLDRRVS---QHSLAEASKLGSKTALNRRDTYRRRQMQRTITRQMSFDLTKLLVTEDWFSDISPQTMRRLLNIVSVTGRLLRANQISFHWDRLASWINLTEQWPYRTSWLILYLEE-TEGVPDQSTLKTIYERISKNIPTT----KDVEPLLEIDSDIRSFEVFLASRTPVLIARDV-KTFLPCTVNLDPKLREIIAD---------VRAARDQMNIGGFPYPTLPLQDAPPRATSVFSQPSSVCSSSSFNGPFPAGVMSPQPPSSYYSGMTG---------------PQHPFYNRATSSSVA----GAHSSALAAMNVDGVCEKLKGIDGIEQSMLPQYTATIRKANINGRVLTQCTMDELKKEMNMNFGDWHLFKSALHEMRSSDTQIQPEEGRMVGEP 1241
BLAST of Kinase D-interacting substrate vs. Ensembl Mouse
Match: Kidins220 (kinase D-interacting substrate 220 [Source:MGI Symbol;Acc:MGI:1924730]) HSP 1 Score: 174.481 bits (441), Expect = 2.904e-43 Identity = 201/853 (23.56%), Postives = 374/853 (43.85%), Query Frame = 2
Query: 428 YIQALADMLVCPNTNFPISIGLFSRVGAGIRTTHLEKLSDELISLTKINNQKTLQPSLLLFMFPXXXXXXXXX--XXXXXTNWIVGVSLGASLSLMSFLLIEIIIFGSNHK--RWGKALRAARWLASFTQSLQLFLDILFRYPHE-NELTTK--PLSIIITHHNLSNYSSASHMTLSIVEKLWKAVEKKYSCLLSRL--QISCKNPESSTKFKNICCIPIFVLVIIFFSAIIINATLFTIYNKNPMDIFVNKGLLPLFVIMTCIVFLCIFGTLPLVCRT----ITFLVRSPSRQLKQFMES-ENIDFQASDTLVKNELLKLCQIISIFEAYLNSEKIRMFVFIDAADTESREKLANSIYLVNGLLQMSSNAPIALILAADFQTLNTEWXXXXXXXXXXXXXXXXIFQEISDNFH-LIHSTIHLPIYMEEGIDFNRNSSGKKKY----ASNG-MFCIRGGNSFESLNMVTTGTKQNSVPVSTR---KTSI--YXXXXXXXXXXXXXKGASKSIQQLFLTRGDLSDINNKMIKQLTAAAAFIGRLLRINLIEFKLENLISWLSISINWPYQTALMALFLEEQCANLTPKHTLLQIHEQVLSKIGSKDSNPAEIELLARDDRDFGKLDAFIKKDVGATLTFSILKYFVSWSPFMNPYLRYVLRDYLTNKNLAQTRGSRNQLT-------------SPPLTSMALSDPSDV-----PMDEDLPQPIISPSTGSGLVNQITSWSKSSIGNLAHKGDRPKTPIAIKRCMVKLKDGYPGKDLSQLDKYEIGILMC----QIEGLSSEENIEKYKEKLIQLNVTGLVLSVCDMDQLQNEMEMNFGDWQLFKTLIYHLRSVE 2845
Y ALAD+L P PI +GL+++ G+G ++ L+KL DE+ + + Q S L+ + L +G+ + TN + VSL + L+L+ I +I FG + W A + LA L+L ++F P E E TTK P+ + T +N + ++ L A E+++ L +RL ++ + K+K CC+P F++ + II TL I+ +P + VN + + + IV L L CRT + L+ S ++L + + ++K E+ + ++ +++ ++ R+ V ID D ++K+ + +++ + + S P I A+D + + + +++ ++ + N H + + +HLP+++ +R S +K+ A+NG + C E + + QNS+ T+ KT++ + + ++ S + +L +T SDI+ + +++L + GRLLR N I F + L SW++++ WPY+T+ + L+L E + + TL I+E++ I + ++E L D D + F+ L +K F+ + ++P LR ++ D R +R Q+ +PP S P+ V + P ++SP S + ++ P+ P R V P S L ++C QIEGL + + +Y + + N+ G VL+ C++D+L+ EM MNFGDW LF++++ +RSVE
Sbjct: 401 YSSALADILSEPTMQPPICVGLYAQWGSG-KSFLLKKLEDEMKTFAGQQIEPLFQFSWLIVFLILLLCGGLGLVFAFTVDTNLAIAVSL-SFLALLYIFFI-VIYFGGRQEGESWNWAWALSTRLARHIGYLELLFKLMFVNPPELPEQTTKALPVRFLFTDYNRLSSVGGETSLAEMIATLSDACEREFGFLATRLFRVFKTEDSQGKKKWKKTCCLPSFIIFLFIVGCIIAGITLLAIFRVDPKHLTVNA----ILISIASIVGLAFV----LNCRTWWQVLDSLLNSQRKRLHSAASKLHKLKSEGFMKVLKCEVELMARMAKTIDSFTQNQ-TRLVVIIDGLDACEQDKV---LQMLDTVRVLFSKGPFIAIFASDPHII----------IKAINQNLNSVLRDSNINGHDYMRNIVHLPVFLN-----SRGLSNARKFLVTSATNGDISCSEATGVQEDADRRVS---QNSLGEMTKLGSKTALNRRDTYRRRQMQRTITRQMSFDLTKLLVTEDWFSDISPQTMRRLLNIVSVTGRLLRANQITFNWDRLASWINLTEQWPYRTSWLILYLGE-TEGIPDQMTLKTIYERISKNIPTT----KDVEPLLEIDGDIRNFEVFLSSRT-PVLVARDVKTFLPCTVNLDPKLREIIAD---------VRAAREQINIGGLAYPPLPLHEAPPRPPSGYSQPASVCSSSASFNGPFPGGVVSPQPHSSYYSGLSG---------------PQHPF-YNRASV------PATGTSLLLSSMTVDIVCEKLRQIEGL-DQGMLPQYCTTIKKANINGRVLAQCNIDELKKEMAMNFGDWHLFRSMVLEMRSVE 1182
BLAST of Kinase D-interacting substrate vs. Ensembl Mouse
Match: Kidins220 (kinase D-interacting substrate 220 [Source:MGI Symbol;Acc:MGI:1924730]) HSP 1 Score: 169.859 bits (429), Expect = 5.796e-42 Identity = 196/867 (22.61%), Postives = 370/867 (42.68%), Query Frame = 2
Query: 428 YIQALADMLVCPNTNFPISIGLFSRVGAGIRTTHLEKLSDELISLTKINNQKTLQPSLLLFMFPXXXXXXXXXXXXXXTNWIVGVSLGASLSLMSFLLIEIIIFGSNHK--RWGKALRAARWLASFTQSLQLFLDILFRYPHE-NELTTK--PLSIIITHHNLSNYSSASHMTLSIVEKLWKAVEKKYSCLLSRL--QISCKNPESSTKFKNICCIPIFVLVIIFFSAIIINATLFTIYNKNPMDIFVNKGLLPLFVIMTCIVFLCIFGTLPLVCRT----ITFLVRSPSRQLKQFMES-ENIDFQASDTLVKNELLKLCQIISIFEAYLNSEKIRMFVFIDAADTESREKLANSIYLVNGLLQMSSNAPIALILAADFQTLNTEWXXXXXXXXXXXXXXXXIFQEISDNFH-LIHSTIHLPIYMEEGIDFNRNSSGKKKY----ASNG-MFCIRGGNSFESLNMVTTGTKQNSVPVSTR---KTSI--YXXXXXXXXXXXXXKGASKSIQQLFLTRGDLSDINNKMIKQLTAAAAFIGRLLRINLIEFKLENLISWLSISINWPYQTALMALFLEEQCANLTPKHTLLQIHEQVLSKIGSKDSNPAEIELLARDDRDFGKLDAFIKKDVGATLTFSILKYFVSWSPFMNPYLRYVLRDYLTNKNLAQTRGSRNQLT-------------SPPLTSMALSDPSDV-----PMDEDLPQPIISPSTGSGLVNQITS----------------WSKSSIGNLAHKGDRPKTPIAIKRCMVKLKDGYPGKDLSQLDKYEIGILMC----QIEGLSSEENIEKYKEKLIQLNVTGLVLSVCDMDQLQNEMEMNFGDWQLFKTLIYHLRSVE 2845
Y ALAD+L P PI +GL+++ G+G ++ L+KL DE+ + + Q S L+ + L +G++ + + +++ S + ++ +I FG + W A + LA L+L ++F P E E TTK P+ + T +N + ++ L A E+++ L +RL ++ + K+K CC+P F++ + II TL I+ +P + VN + + + IV L L CRT + L+ S ++L + + ++K E+ + ++ +++ ++ R+ V ID D ++K+ + V L S P I A+D + + + +++ ++ + N H + + +HLP+++ +R S +K+ A+NG + C E + + QNS+ T+ KT++ + + ++ S + +L +T SDI+ + +++L + GRLLR N I F + L SW++++ WPY+T+ + L+L E + + TL I+E++ I + ++E L D D + F+ L +K F+ + ++P LR ++ D R +R Q+ +PP S P+ V + P ++SP S + ++ + G H R ++ R P S L ++C QIEGL + + +Y + + N+ G VL+ C++D+L+ EM MNFGDW LF++++ +RSVE
Sbjct: 273 YSSALADILSEPTMQPPICVGLYAQWGSG-KSFLLKKLEDEMKTFAGQQIEPLFQFSWLIVFLILLLCGGLGLVFAFTVDTNLAIAVSLSFLALLYIFFIVIYFGGRQEGESWNWAWALSTRLARHIGYLELLFKLMFVNPPELPEQTTKALPVRFLFTDYNRLSSVGGETSLAEMIATLSDACEREFGFLATRLFRVFKTEDSQGKKKWKKTCCLPSFIIFLFIVGCIIAGITLLAIFRVDPKHLTVNA----ILISIASIVGLAFV----LNCRTWWQVLDSLLNSQRKRLHSAASKLHKLKSEGFMKVLKCEVELMARMAKTIDSFTQNQT-RLVVIIDGLDACEQDKVLQMLDTVRVLF---SKGPFIAIFASDPHII----------IKAINQNLNSVLRDSNINGHDYMRNIVHLPVFLN-----SRGLSNARKFLVTSATNGDISCSEATGVQEDADRRVS---QNSLGEMTKLGSKTALNRRDTYRRRQMQRTITRQMSFDLTKLLVTEDWFSDISPQTMRRLLNIVSVTGRLLRANQITFNWDRLASWINLTEQWPYRTSWLILYLGE-TEGIPDQMTLKTIYERISKNIPTTK----DVEPLLEIDGDIRNFEVFLSSRT-PVLVARDVKTFLPCTVNLDPKLREIIAD---------VRAAREQINIGGLAYPPLPLHEAPPRPPSGYSQPASVCSSSASFNGPFPGGVVSPQPHSSYYSGLSGPQHPFYNRPFFAPYLYTPRYYPGGSQHLISRSSVKTSLPRDQNNGLASVPATGTSLLLSSMTVDIVCEKLRQIEGL-DQGMLPQYCTTIKKANINGRVLAQCNIDELKKEMAMNFGDWHLFRSMVLEMRSVE 1092
BLAST of Kinase D-interacting substrate vs. Ensembl Mouse
Match: Kidins220 (kinase D-interacting substrate 220 [Source:MGI Symbol;Acc:MGI:1924730]) HSP 1 Score: 166.392 bits (420), Expect = 8.686e-41 Identity = 194/878 (22.10%), Postives = 375/878 (42.71%), Query Frame = 2
Query: 428 YIQALADMLVCPNTNFPISIGLFSRVGAGIRTTHLEKLSDELISLTKINNQKTLQPSLLLFMFPXXXXXXXXXXXXXXTNWIVGVSLGASLSLMSFLLIEIIIFGSNHK--RWGKALRAARWLASFTQSLQLFLDILFRYPHE-NELTTK--PLSIIITHHNLSNYSSASHMTLSIVEKLWKAVEKKYSCLLSRL--QISCKNPESSTKFKNICCIPIFVLVIIFFSAIIINATLFTIYNKNPMDIFVNKGLLPLFVIMTCIVFLCIFGTLPLVCRT----ITFLVRSPSRQLKQFMES-ENIDFQASDTLVKNELLKLCQIISIFEAYLNSEKIRMFVFIDAADTESREKLANSIYLVNGLLQMSSNAPIALILAADFQTLNTEWXXXXXXXXXXXXXXXXIFQEISDNFH-LIHSTIHLPIYMEEGIDFNRNSSGKKKY----ASNG-MFCIRGGNSFESLNMVTTGTKQNSVPVSTR---KTSI--YXXXXXXXXXXXXXKGASKSIQQLFLTRGDLSDINNKMIKQLTAAAAFIGRLLRINLIEFKLENLISWLSISINWPYQTALMALFLEEQCANLTPKHTLLQIHEQVLSKIGSKDSNPAEIELLARDDRDFGKLDAFIKKDVGATLTFSILKYFVSWSPFMNPYLRYVLRDYLTNKNLAQTRGSRNQLT-------------SPPLTSMALSDPSDV-----PMDEDLPQPIISPSTGSGLVNQITS----------------WSKSSIGNLAHKGDRPKTPIAIKRCM-----------VKLKDGYPGKDLSQLDKYEIGILMC----QIEGLSSEENIEKYKEKLIQLNVTGLVLSVCDMDQLQNEMEMNFGDWQLFKTLIYHLRSVE 2845
Y ALAD+L P PI +GL+++ G+G ++ L+KL DE+ + + Q S L+ + L +G++ + + +++ S + ++ +I FG + W A + LA L+L ++F P E E TTK P+ + T +N + ++ L A E+++ L +RL ++ + K+K CC+P F++ + II TL I+ +P + VN + + + IV L L CRT + L+ S ++L + + ++K E+ + ++ +++ ++ R+ V ID D ++K+ + +++ + + S P I A+D + + + +++ ++ + N H + + +HLP+++ +R S +K+ A+NG + C E + + QNS+ T+ KT++ + + ++ S + +L +T SDI+ + +++L + GRLLR N I F + L SW++++ WPY+T+ + L+L E + + TL I+E++ I + ++E L D D + F+ L +K F+ + ++P LR ++ D R +R Q+ +PP S P+ V + P ++SP S + ++ + G H R ++ R + + P S L ++C QIEGL + + +Y + + N+ G VL+ C++D+L+ EM MNFGDW LF++++ +RSVE
Sbjct: 443 YSSALADILSEPTMQPPICVGLYAQWGSG-KSFLLKKLEDEMKTFAGQQIEPLFQFSWLIVFLILLLCGGLGLVFAFTVDTNLAIAVSLSFLALLYIFFIVIYFGGRQEGESWNWAWALSTRLARHIGYLELLFKLMFVNPPELPEQTTKALPVRFLFTDYNRLSSVGGETSLAEMIATLSDACEREFGFLATRLFRVFKTEDSQGKKKWKKTCCLPSFIIFLFIVGCIIAGITLLAIFRVDPKHLTVNA----ILISIASIVGLAFV----LNCRTWWQVLDSLLNSQRKRLHSAASKLHKLKSEGFMKVLKCEVELMARMAKTIDSFTQNQ-TRLVVIIDGLDACEQDKV---LQMLDTVRVLFSKGPFIAIFASDPHII----------IKAINQNLNSVLRDSNINGHDYMRNIVHLPVFLN-----SRGLSNARKFLVTSATNGDISCSEATGVQEDADRRVS---QNSLGEMTKLGSKTALNRRDTYRRRQMQRTITRQMSFDLTKLLVTEDWFSDISPQTMRRLLNIVSVTGRLLRANQITFNWDRLASWINLTEQWPYRTSWLILYLGE-TEGIPDQMTLKTIYERISKNIPTT----KDVEPLLEIDGDIRNFEVFLSSRT-PVLVARDVKTFLPCTVNLDPKLREIIAD---------VRAAREQINIGGLAYPPLPLHEAPPRPPSGYSQPASVCSSSASFNGPFPGGVVSPQPHSSYYSGLSGPQHPFYNRPFFAPYLYTPRYYPGGSQHLISRSSVKTSLPRDQNNGLLCDSGFNKQRQASVPATGTSLLLSSMTVDIVCEKLRQIEGLD-QGMLPQYCTTIKKANINGRVLAQCNIDELKKEMAMNFGDWHLFRSMVLEMRSVE 1273
BLAST of Kinase D-interacting substrate vs. Ensembl Mouse
Match: Kidins220 (kinase D-interacting substrate 220 [Source:MGI Symbol;Acc:MGI:1924730]) HSP 1 Score: 134.806 bits (338), Expect = 4.254e-31 Identity = 165/708 (23.31%), Postives = 312/708 (44.07%), Query Frame = 2
Query: 428 YIQALADMLVCPNTNFPISIGLFSRVGAGIRTTHLEKLSDELISLTKINNQKTLQPSLLLFMFPXXXXXXXXX--XXXXXTNWIVGVSLGASLSLMSFLLIEIIIFGSNHK--RWGKALRAARWLASFTQSLQLFLDILFRYPHE-NELTTK--PLSIIITHHNLSNYSSASHMTLSIVEKLWKAVEKKYSCLLSRL--QISCKNPESSTKFKNICCIPIFVLVIIFFSAIIINATLFTIYNKNPMDIFVNKGLLPLFVIMTCIVFLCIFGTLPLVCRT----ITFLVRSPSRQLKQFMES-ENIDFQASDTLVKNELLKLCQIISIFEAYLNSEKIRMFVFIDAADTESREKLANSIYLVNGLLQMSSNAPIALILAADFQTLNTEWXXXXXXXXXXXXXXXXIFQEISDNFH-LIHSTIHLPIYMEEGIDFNRNSSGKKKY----ASNG-MFCIRGGNSFESLNMVTTGTKQNSVPVSTR---KTSI--YXXXXXXXXXXXXXKGASKSIQQLFLTRGDLSDINNKMIKQLTAAAAFIGRLLRINLIEFKLENLISWLSISINWPYQTALMALFLEEQCANLTPKHTLLQIHEQVLSKIGSKDSNPAEIELLARDDRDFGKLDAFIKKDVGATLTFSILKYFVSWSPFMNPYLRYVLRDYLTNKNLAQTRGSRNQ-----LTSPPLTSMALSDPSDVPMDEDLPQP 2461
Y ALAD+L P PI +GL+++ G+G ++ L+KL DE+ + + Q S L+ + L +G+ + TN + VSL + L+L+ I +I FG + W A + LA L+L ++F P E E TTK P+ + T +N + ++ L A E+++ L +RL ++ + K+K CC+P F++ + II TL I+ +P + VN + + + IV L L CRT + L+ S ++L + + ++K E+ + ++ +++ ++ R+ V ID D ++K+ + +++ + + S P I A+D + + + +++ ++ + N H + + +HLP+++ +R S +K+ A+NG + C E + + QNS+ T+ KT++ + + ++ S + +L +T SDI+ + +++L + GRLLR N I F + L SW++++ WPY+T+ + L+L E + + TL I+E++ I + ++E L D D + F+ L +K F+ + ++P LR ++ D R +R Q L PPL P+ E P+P
Sbjct: 443 YSSALADILSEPTMQPPICVGLYAQWGSG-KSFLLKKLEDEMKTFAGQQIEPLFQFSWLIVFLILLLCGGLGLVFAFTVDTNLAIAVSL-SFLALLYIFFI-VIYFGGRQEGESWNWAWALSTRLARHIGYLELLFKLMFVNPPELPEQTTKALPVRFLFTDYNRLSSVGGETSLAEMIATLSDACEREFGFLATRLFRVFKTEDSQGKKKWKKTCCLPSFIIFLFIVGCIIAGITLLAIFRVDPKHLTVNA----ILISIASIVGLAFV----LNCRTWWQVLDSLLNSQRKRLHSAASKLHKLKSEGFMKVLKCEVELMARMAKTIDSFTQNQ-TRLVVIIDGLDACEQDKV---LQMLDTVRVLFSKGPFIAIFASDPHII----------IKAINQNLNSVLRDSNINGHDYMRNIVHLPVFLN-----SRGLSNARKFLVTSATNGDISCSEATGVQEDADRRVS---QNSLGEMTKLGSKTALNRRDTYRRRQMQRTITRQMSFDLTKLLVTEDWFSDISPQTMRRLLNIVSVTGRLLRANQITFNWDRLASWINLTEQWPYRTSWLILYLGE-TEGIPDQMTLKTIYERISKNIPTT----KDVEPLLEIDGDIRNFEVFLSSRT-PVLVARDVKTFLPCTVNLDPKLREIIAD---------VRAAREQINIGGLAYPPL-----------PLHEAPPRP 1091 HSP 2 Score: 63.1586 bits (152), Expect = 3.508e-9 Identity = 27/61 (44.26%), Postives = 43/61 (70.49%), Query Frame = 2
Query: 2663 QIEGLSSEENIEKYKEKLIQLNVTGLVLSVCDMDQLQNEMEMNFGDWQLFKTLIYHLRSVE 2845
QIEGL + + +Y + + N+ G VL+ C++D+L+ EM MNFGDW LF++++ +RSVE
Sbjct: 1244 QIEGLD-QGMLPQYCTTIKKANINGRVLAQCNIDELKKEMAMNFGDWHLFRSMVLEMRSVE 1303
BLAST of Kinase D-interacting substrate vs. UniProt/SwissProt
Match: sp|Q7T163|KDISB_DANRE (Kinase D-interacting substrate of 220 kDa B OS=Danio rerio OX=7955 GN=kidins220b PE=1 SV=3) HSP 1 Score: 177.178 bits (448), Expect = 3.450e-43 Identity = 204/840 (24.29%), Postives = 374/840 (44.52%), Query Frame = 2
Query: 428 YIQALADMLVCPNTNFPISIGLFSRVGAGIRTTHLEKLSDELISLTKINNQKTLQPSLLLFMFPXXXXXXXXXXXXXXTNWIVGVSLGASLSLMSFLLIEIIIFGSNHK--RWGKALRAARWLASFTQSLQLFLDILFRYPHE-NELTTK--PLSIIITHHN-LSNYSSASHMTLSIVEKLWKAVEKKYSCLLSRL--QISCKNPESSTKFKNICCIPIFVLVIIFFSAIIINATLFTIYNKNPMDIFVNKGLLPLFVIMTCIVFLCIFGTLPLVCRTITFLVRSP-SRQLKQFMESEN----IDFQASDTLVKNELLKLCQIISIFEAYLNSEKIRMFVFIDAADTESREKLANSIYLVNGLLQMSSNAPIALILAADFQTLNTEWXXXXXXXXXXXXXXXXIFQEISDNFH-LIHSTIHLPIYMEEGIDFNRNSSGKKKYASNGMFCIRGGNSFESLNMVTTGTKQNSVPVSTR---KTSI--YXXXXXXXXXXXXXKGASKSIQQLFLTRGDLSDINNKMIKQLTAAAAFIGRLLRINLIEFKLENLISWLSISINWPYQTALMALFLEEQCANLTPKHTLLQ-IHEQVLSKIGSKDSNPAEIELLARDDRDFGKLDAFIKKDVGATLTFSILKYFVSWSPFMNPYLRYVLRDYLTNKNLAQTRGSRNQLTSPPLTSMALSDPSDVPMDEDLPQPIIS--------------PSTGSGLVNQITSWSKSSIGNLAHKGDRPKTPIAIKRCMVKLKDGYPGKDLSQLDKYEIGILMCQIEGLSSEENIEKYKEKLIQLNVTGLVLSVCDMDQLQNEMEMNFGDWQLFKTLIYHLRSVE 2845
Y ALAD+L P PI +GL+++ G+G ++ L+KL DE+ + + Q S L+ + + L V +++G + + +++ S+ + ++ ++ FGS + W A + LA L+L L ++F P E E TT+ P+ + T +N LS+ + M ++ L A E+++ L +RL ++ + K+K CCIP FV+ + +I+ L ++ + + VN + V M +V L + L CRT + S + Q K+ + N + + ++KNE+ + ++ + + ++ R+ V ID D+ ++K+ + V L S P I A+D + + + +++ ++ + N H + + +HLP+++ +R S KK + GNS + QNS+ T+ KT++ + S ++ S + +L +T SDI+ + +++L + GRLLR N I F + L SW++++ WPY+T+ + L+LEE + P T L+ I+E++ I + ++E L D D + F+ L ++ F+ + ++P LR ++ D R +R Q+ +T L P+ E P + S P G+ Q S S + P+ P R + G P LS + I + I+G+ + I +Y + + N+ G VLS C++D+L+ EM MNFGDWQLF+T + +R VE
Sbjct: 452 YSSALADILSEPTMQPPICVGLYAQWGSG-KSFLLKKLEDEMKTFAGQQVEPLFQFSWLVVLLSLLLCGSVALVLGFTVDPKLAIAISLSILALLYVFFVVVYFGSRREGESWNWAWVISTRLARHIGYLELLLKLMFVNPPELPEQTTRALPVRFLFTDYNRLSSVGGETSMA-EMIATLSDACEREFGFLATRLFRVFKTEDTQGKKKWKKTCCIPSFVIFLFILGCLIMGMALLAVFKVDGQNQTVNA----VLVSMASVVGLALL----LNCRTWWQVTDSVLNSQRKRLHSAANKMHKLKSEGFMKVLKNEVELMAKMAKTIDGFTQNQ-TRLVVIIDGLDSCEQDKVLQMLDTVRVLF---SKGPFISIFASDPHII----------IKAINQNLNSVLRDSNINGHDYMRNIVHLPVFLN-----SRGLSSAKKMCAPAPANGETGNSEGWHEELDRKLSQNSLGDQTKFGSKTTLNRRDTYRRRQMQRSVTRQMSFDLTKLLVTEDWFSDISPQTMRRLLNIVSVTGRLLRANQISFNWDRLASWINLTEQWPYRTSWLILYLEE--TDGIPDQTNLKTIYERISKNIPTT----KDVEPLLEIDGDVRSFEVFLSSRT-PVLAARDIRTFLPCTVNLDPKLREIIAD---------VRAAREQVNMAGVTYPTL------PLQEGRPISMYSQQSSACSPTASFNGPYNPPGVSPQPHSAYFSGMAG-------PQHPF-YNRGSASVVSGTPSILLSSMSTDVICERVKLIDGID-QNLISQYTATIKKANINGRVLSQCNIDELKKEMNMNFGDWQLFRTSVLEMRHVE 1231
BLAST of Kinase D-interacting substrate vs. UniProt/SwissProt
Match: sp|Q9ULH0|KDIS_HUMAN (Kinase D-interacting substrate of 220 kDa OS=Homo sapiens OX=9606 GN=KIDINS220 PE=1 SV=3) HSP 1 Score: 167.933 bits (424), Expect = 2.201e-40 Identity = 196/887 (22.10%), Postives = 372/887 (41.94%), Query Frame = 2
Query: 428 YIQALADMLVCPNTNFPISIGLFSRVGAGIRTTHLEKLSDELISLTKINNQKTLQPSLLLFMFPXXXXXXXXXXXXXXTNWIVGVSLGASLSLMSFLLIEIIIFGSNHK--RWGKALRAARWLASFTQSLQLFLDILFRYPHE-NELTTK--PLSIIITHHNLSNYSSASHMTLSIVEKLWKAVEKKYSCLLSRL--QISCKNPESSTKFKNICCIPIFVLVIIFFSAIIINATLFTIYNKNPMDIFVNKGLLPLFVIMTCIVFLCIFGTLPLVCRT----ITFLVRSPSRQLKQFMES-ENIDFQASDTLVKNELLKLCQIISIFEAYLNSEKIRMFVFIDAADTESREKLANSIYLVNGLLQMSSNAPIALILAADFQTLNTEWXXXXXXXXXXXXXXXXIFQEISDNFH-LIHSTIHLPIYMEEGIDFNRNSSGKKKY----ASNGMFCIRGGNSFE-------SLNMVTTGTKQNSVPVSTRKTSIYXXXXXXXXXXXXXKGASKSIQQLFLTRGDLSDINNKMIKQLTAAAAFIGRLLRINLIEFKLENLISWLSISINWPYQTALMALFLEEQCANLTPKHTLLQIHEQVLSKIGSKDSNPAEIELLARDDRDFGKLDAFIKKDVGATLTFSILKYFVSWSPFMNPYLRYVLRDYLTNKNLAQTRGSRNQLT-------------SPPLTSMALSDPSDVPMDEDLPQP----IISPSTGSGLVNQITS----------------WSKSSIGNLAHKGDRPKTPIAIKR-----CMVKLKDGYPGKD------------------LSQLDKYEIGILMCQIEGLSSEENIEKYKEKLIQLNVTGLVLSVCDMDQLQNEMEMNFGDWQLFKTLIYHLRSVEA 2848
Y ALAD+L P PI +GL+++ G+G ++ L+KL DE+ + + Q S L+ + L +G++ + +G+++ S + ++ +I FG + W A + LA L+L L ++F P E E TTK P+ + T +N + ++ L A E+++ L +RL ++ + K+K CC+P FV+ + II TL I+ +P + VN + + + +V L L CRT + L+ S ++L + + ++K E+ + ++ +++ ++ R+ V ID D ++K+ + +++ + + S P I A+D + + + +++ ++ + N H + + +HLP+++ +R S +K+ A+NG + S N + TK S R+ + + ++ S + +L +T SDI+ + +++L + GRLLR N I F + L SW++++ WPY+T+ + L+LEE + + TL I+E++ I + ++E L D D + F+ L +K F+ + ++P LR ++ D R +R Q++ PP S P V P ++SP S + +T + G H RP ++ R V +D G L+ L+ + + QIEGL + + +Y + + N+ G VL+ C++D+L+ EM MNFGDW LF++ + +R+ E+
Sbjct: 443 YSSALADILSEPTMQPPICVGLYAQWGSG-KSFLLKKLEDEMKTFAGQQIEPLFQFSWLIVFLTLLLCGGLGLLFAFTVHPNLGIAVSLSFLALLYIFFIVIYFGGRREGESWNWAWVLSTRLARHIGYLELLLKLMFVNPPELPEQTTKALPVRFLFTDYNRLSSVGGETSLAEMIATLSDACEREFGFLATRLFRVFKTEDTQGKKKWKKTCCLPSFVIFLFIIGCIISGITLLAIFRVDPKHLTVNA----VLISIASVVGLAFV----LNCRTWWQVLDSLLNSQRKRLHNAASKLHKLKSEGFMKVLKCEVELMARMAKTIDSFTQNQ-TRLVVIIDGLDACEQDKV---LQMLDTVRVLFSKGPFIAIFASDPHII----------IKAINQNLNSVLRDSNINGHDYMRNIVHLPVFLN-----SRGLSNARKFLVTSATNGDVPCSDTTGIQEDADRRVSQNSLGEMTKLGSKTALNRRDTY----RRRQMQRTITRQMSFDLTKLLVTEDWFSDISPQTMRRLLNIVSVTGRLLRANQISFNWDRLASWINLTEQWPYRTSWLILYLEE-TEGIPDQMTLKTIYERISKNIPTT----KDVEPLLEIDGDIRNFEVFLSSRT-PVLVARDVKVFLPCTVNLDPKLREIIAD---------VRAAREQISIGGLAYPPLPLHEGPPRAPSGYSQPPSVCSSTSFNGPFAGGVVSPQPHSSYYSGMTGPQHPFYNRPFFAPYLYTPRYYPGGSQHLISRPSVKTSLPRDQNNGLEVIKEDAAEGLSSPTDSSRGSGPAPGPVVLLNSLNVDAVCEKLKQIEGL-DQSMLPQYCTTIKKANINGRVLAQCNIDELKKEMNMNFGDWHLFRSTVLEMRNAES 1281
BLAST of Kinase D-interacting substrate vs. UniProt/SwissProt
Match: sp|Q9EQG6|KDIS_RAT (Kinase D-interacting substrate of 220 kDa OS=Rattus norvegicus OX=10116 GN=Kidins220 PE=1 SV=2) HSP 1 Score: 140.584 bits (353), Expect = 4.895e-32 Identity = 164/706 (23.23%), Postives = 308/706 (43.63%), Query Frame = 2
Query: 428 YIQALADMLVCPNTNFPISIGLFSRVGAGIRTTHLEKLSDELISLTKINNQKTLQPSLLLFMFPXXXXXXXXXXXXXXTNWIVGVSLGASLSLMSFLLIEIIIFGSNHK--RWGKALRAARWLASFTQSLQLFLDILFRYPHE-NELTTK--PLSIIITHHNLSNYSSASHMTLSIVEKLWKAVEKKYSCLLSRLQISCKNPESS--TKFKNICCIPIFVLVIIFFSAIIINATLFTIYNKNPMDIFVNKGLLPLFVIMTCIVFLCIFGTLPLVCRT----ITFLVRSPSRQLKQFMES-ENIDFQASDTLVKNELLKLCQIISIFEAYLNSEKIRMFVFIDAADTESREKLANSIYLVNGLLQMSSNAPIALILAADFQTLNTEWXXXXXXXXXXXXXXXXIFQEISDNFH-LIHSTIHLPIYMEEGIDFNRNSSGKKKY----ASNG-MFCIRGGNSFESLNMVTTGTKQNSVPVSTR---KTSI--YXXXXXXXXXXXXXKGASKSIQQLFLTRGDLSDINNKMIKQLTAAAAFIGRLLRINLIEFKLENLISWLSISINWPYQTALMALFLEEQCANLTPKHTLLQIHEQVLSKIGSKDSNPAEIELLARDDRDFGKLDAFIKKDVGATLTFSILKYFVSWSPFMNPYLRYVLRDYLTNKNLAQTRGSRNQ-----LTSPPLTSMALSDPSDVPMDEDLPQP 2461
Y ALAD+L P PI +GL+++ G+G ++ L+KL DE+ + + Q S L+ + L +G++ + + +++ S + ++ +I FG + W A + LA L+L ++F P E E TTK P+ + T +N + ++ L A E+++ L +RL + ES K+K CC+P FV+ + II TL I+ +P + VN + + + +V L L CRT + L+ S ++L + + ++K E+ + ++ +++ ++ R+ V ID D ++K+ + V L S P I A+D + + + +++ ++ + N H + + +HLP+++ +R S +K+ A+NG + C + E + + QNS+ T+ KT++ + + ++ S + +L +T SDI+ + +++L + GRLLR N I F + L SW++++ WPY+T+ + L+LEE L + TL I+E++ I + ++E L D D + F+ L +K F+ + ++P LR ++ D R +R Q L PPL P+ E P+P
Sbjct: 443 YSSALADILSEPTMQPPICVGLYAQWGSG-KSFLLKKLEDEMKTFAGQQTEPLFQFSWLIVFLTLLLCGGLGLVFAFTVDTNLAIAISLSFLALIYIFFIVIYFGGRREGESWNWAWALSTRLARHIGYLELLFKLMFVNPPELPEQTTKALPVRFLFTDYNRLSSVGGETSLAEMIATLSDACEREFGFLATRLFRVFRTEESQGKKKWKKTCCLPSFVIFLFIVGCIIAGITLLAIFRVDPKHLTVNA----ILISIASVVGLAFV----LNCRTWWQVLDSLLNSQRKRLHSAASKLHKLKSEGFMKVLKCEVELMARMAKTIDSFTQNQ-TRLVVIIDGLDACEQDKVLQMLDTVRVLF---SKGPFIAIFASDPHII----------IKAINQNLNSVLRDSNINGHDYMRNIVHLPVFLN-----SRGLSNARKFLVTSATNGDITCSDTTGTQEDTDRRVS---QNSLGEMTKLGSKTALNRRDTYRRRQMQRTITRQMSFDLTKLLVTEDWFSDISPQTMRRLLNIVSVTGRLLRANQITFNWDRLASWINLTEQWPYRTSWLILYLEE-TEGLPDQMTLKTIYERISKNIPTT----KDVEPLLEIDGDIRNFEVFLSSRT-PVLVARDVKTFLPCTVNLDPKLREIIAD---------VRAAREQINIGGLAYPPL-----------PLHEGPPRP 1091 HSP 2 Score: 64.6994 bits (156), Expect = 7.511e-9 Identity = 28/62 (45.16%), Postives = 44/62 (70.97%), Query Frame = 2
Query: 2663 QIEGLSSEENIEKYKEKLIQLNVTGLVLSVCDMDQLQNEMEMNFGDWQLFKTLIYHLRSVEA 2848
QIEGL + + +Y + + N+ G VLS C++D+L+ EM MNFGDW LF++++ +RSVE+
Sbjct: 1213 QIEGLD-QSMMPQYCTTIKKANINGRVLSQCNIDELKKEMAMNFGDWHLFRSMVLEMRSVES 1273
BLAST of Kinase D-interacting substrate vs. TrEMBL
Match: A0A4Z2DVE7 (Kinase D-interacting substrate OS=Schistosoma japonicum OX=6182 GN=EWB00_004527 PE=4 SV=1) HSP 1 Score: 301.212 bits (770), Expect = 2.045e-79 Identity = 253/952 (26.58%), Postives = 451/952 (47.37%), Query Frame = 2
Query: 344 VEDFIGLNYFNYPLDHCGSNIRAQKTVTYIQALADMLVCPNTNFPISIGLFSRVGAGIRTTHLEKLSDELISLTKINNQKTLQPSLLLFMFPXXXXXXXXXXXXXXTNWIVGVSLGASLSLMSFLLIEIIIFGSNHKRWGKALRAARWLASFTQSLQLFLDILFRYPHENELTTKPLSIIITHHNLSNYSSASHMTLSIVEKLWKAVEKKYSCLLSRLQISCKNPES-STKFKNICCIPIFVLVIIFFSAIIINATLFTIYNKNPMDIFVNKGLLPLFVIMTCIVFLCIFGTLPLVCRTITFLVRSP----SRQLKQFMESEN-----------------IDFQAS-------------------DTLVKNELLKLCQIISIFEAYLNSEKIRMFVFIDAADTESREKLANSIYLVNGLLQMSSNAPIALILAADFQTLNTEWXXXXXXXXXXXX-------XXXXIFQEISDNFHLIHSTIHLPIYMEEGI--DFNRNSSGKKKYASNGMFCIRGGNSFESL------NMVTTGTKQNSVPVSTRKTSI-----------------YXXXXXXXXXXXXXKGASKS------------------IQQLFLTRGDLSDINNKMIKQLTAAAAFIGRLLRINLIEFKLENLISWLSISINWPYQTALMALFLEEQCANLTPKHTLLQIHE------QVLSKIGSKDSNPAEI-ELLARDDRDFGKLDAFIKKDVGATLTFSILKYFVSWSPFMNPYLRYVLRDYLTNKNLAQTRGSRNQLTSPPLTSMALSDPSDVPMDEDLPQ----------PIISPSTGSGLVNQITSWSKSSIGNLAHKGDR-----PKTPIAI------KRCMVKLKDGYPGKDLSQLDKYEIGILMCQIEGLSSEENIEKYKEKLIQLNVTGLVLSVCDMDQLQNEMEMNFGDWQLFKTLIYHLRSV 2842
+E F +N P+++ ++ T I+ LADM C +NFP+++G+FS+ +L L+ EL +L + N L+ S LLF+FP+ + ++G VG+ TNW VG+S+G + + +II GS A+RA+ +A F ++LFL + F YP ++ T P+ +I+ +H + S+ + S+VEK+W + + Y +L RLQ + K S S K+K +CC+P FV++I F+ + +I+ ++ +Y+ + + GLL LFVIM+ I+FL FG+LP + R I L+ +P L F+++ N F AS D VK+E K+C + F+ + N+++ R + +DA+ T ++ LA IY ++ L+ +AP+A++L A+F+ L + S+ + ++ I Q +SDN HL+H+++HLPIY+E I DFN + +++ N + + ++TG N + ++RK S Y + +N+ S++ I ++FL +L+D N K+IKQL + F RLL++ + + +++W+++ +WPY T + ++LEE + + Q HE + +I + S EI E LA +DRD +L+ F+ + + LK ++ + NP++RY ++ + + N Q+ G ++ + PL+S+ + + L + I +T NQ+ + SS+ + + P IA K C + LK YP ++ ++ L I G +++ Y K+ QLN+ G+VLSVCD+++L+ E+ +N DWQ L+ LRS+
Sbjct: 43 LESFADTGAYNIPIENNSPDVNNHAANT-IRVLADMFTCRLSNFPMTVGIFSQQN---NKEYLSHLASELHNLDRFGNPIPLKFSKLLFIFPLVISAILGYFVGMLTNWQVGLSIGHIGMFIQITFLALIITGSRFCHKPMAIRASYSVACFLHWVKLFLSVCFCYPIKSVNTKTPVRLIVVYHRICPSSTTNIALFSLVEKMWFGLLRHYGQILIRLQRAGKPSGSPSCKYKRVCCLPAFVVMIAFWISFLISTSIMRLYSGQLAKLLGHGGLLSLFVIMSSIMFLSFFGSLPSLFRIIKGLLINPITPVKSALAAFLQTNNEMDKEFYNTKSAGKVNEAAFLASSTSFKDLAISAEEQRACKADLFVKSEFAKICNLSVTFDRFTNTQQTRFILCLDASSTSQKDLLAKLIYQIHNLILSDISAPVAVVLEANFKILLGDLVCSAILSSYSAAFCSNLTLEPKLITQIVSDNVHLVHASLHLPIYLEPPIFTDFNELTKSYNEHSKQNQHSHSSLNDYSPMIPTLQPKTISTGVISN-INTTSRKVSCNPNCISSNIPRAMFNNSYGTHMNNQQKSKIIPVNSETEQNKIDFTGEYEIINFSDIARMFLHNHELADWNGKVIKQLVLSTTFTYRLLKLYNMNIETGLIVTWITLVHHWPYHTTWLIIYLEELNTSNSMNSNNEQNHEVKINLMEAFLEIKKRVSESIEILESLAIEDRDLERLNKFLYSKTSTQIPLNTLKQLIACTLIHNPFIRYSIQALVGSMNRRQSSGLQHGFVN-PLSSLVEEKSGNAIYNISLDKFGQSSSVTVNTSIQSNTSKVQANQMGCKTASSLSSAENTPSNTYWPCPSDTIAAGDNALNKSCQLTLKKMYPTLPFNKFTVNDVCELFSSI-GSIHPTHLQSYISKIKQLNINGIVLSVCDLEKLKQELMINTEDWQQIYKLLTWLRSL 987
BLAST of Kinase D-interacting substrate vs. TrEMBL
Match: A0A504YHK8 (Uncharacterized protein OS=Fasciola gigantica OX=46835 GN=FGIG_03514 PE=4 SV=1) HSP 1 Score: 268.47 bits (685), Expect = 5.295e-69 Identity = 249/958 (25.99%), Postives = 438/958 (45.72%), Query Frame = 2
Query: 335 KNNVEDFIGLNYFNYPLDHCGSNIRAQKTVTYIQALADMLVCPNTNFPISIGLFSRVGAGIRTTHLEKLSDELISLTKINNQKTLQPSLLLFMFPXXXXXXXXXXXXXXTNWIVGVSLGASLSLMSFLLIEIIIFGSNHKRWGKALRAARWLASFTQSLQLFLDILFRYPHENELTTKPLSIIITHHNLSNYSSASHMTLSIVEKLWKAVEKKYSCLLSRLQISCK-NPESSTKFKNICCIPIFVLVIIFFSAIIINATLFTIYNKNPMDIFVNKGLLPLFVIMTCIVFLCIFGTLPLVCRTITFLVRSPSRQLK----QFMESE--------------------------------------NIDFQA-------SDTLVKNELLKLCQIISIFEAYLNSEKIRMFVFIDAADTESREKLANSIYLVNGLLQMSSNAPIALILAADFQTLNTEWXXXXXXXXXXXXXXXXIFQE----ISDNFHLIHSTIHLPIYMEEGI-----------DFNRNS---------------SGKKK----YASNGMFCIRG------GNSFESLNMVTTGTKQNSVPVSTRKTSIYXXXXXXXXXXXXXKGASKSIQQLFLTRGDLSDINNKMIKQLTAAAAFIGRLLRINLIEFKLENLISWLSISINWPYQTALMALFLE---EQCANLTPKH-------TLLQIHEQVLSKIGSKDSNPAEIELLARDDRDFGKLDAFIKKDVGATLTFSILKYFVSWSPFMNPYLRYVLRDYLTNKNLAQT----RGSRNQLTSPPLTSMALSDPSDVPMDEDLPQPIISPSTGSGLVNQITSWSKSSIGNLAHK-GDRPKTPIAIKRCMVKL--------KDGYPG--KDLSQ---LDKYEIGILMCQIEGLSS--EENIEKYKEKLIQLNVTGLVLSVCDMDQLQNEMEMNFGDWQLFKTLIYHLRSVEA 2848
+N+++ + L F+ PLD ++++ T T I+ALADM C TNFP++ G+F+ +L +L+ EL ++++++ L+ S+LLF+FP+F+ +G +VG+ T W VG+S+G + + III GS A R + +A F + LQL L++ F YP + T P+ I+ +H L ++ + S+V+K+W + + + + RLQ + K +P + +FK +CC+P F++V+ F + + + Y+ + KG + +FVI + I+FL +FG++P + R I L +P + L+ F+ E N+ A +D L+K +LC++ F+ +L+ ++ R + +DA++T+ + LA IY ++ L+ S AP+A +L A+F+ L + +S+ P + + +E +SDN HLI+S+IHLPIY+E + D NS S K+ Y +F RG G +S + TG +S +R + I LFL +L+D N KM+KQL +A +F R LR+ +E +E +I W+++ WPY A + ++LE E + T K L+ ++V +++ + ++E L DDRD +L+ +++ ++ LK +S NP+LR+ +R + N + RG SP LT++ V Q I S+G ++N+ S S G L+ G + C L + Y G K++ Q L +Y + + I+ + E+ Y+ + NV GLV+ D ++L++E+ MN DWQ F + +L E+
Sbjct: 37 RNDLDTLLELGAFHCPLDLITTDVKNHGTNT-IRALADMFTCRLTNFPVTTGIFTTQND---KEYLSRLASELRNISRLSGPVALRFSVLLFLFPLFVSTFLGYLVGMLTTWQVGLSIGHVGMFLQITFLVIIITGSRVCHNHIATRISHSVAQFLRWLQLILNVCFCYPTQQIDTKAPVKTIVVYHRLWPSTTPNVALFSMVDKMWTGLIRMHGEISIRLQRASKPSPLPNRRFKRVCCLPAFLVVLAFCVSFMTETGMIRYYSGEFTAMLTEKGTIVIFVISSSILFLGLFGSIPSLYRLIRSLTINPVQPLRTALADFLSDEANEEEYSHLRPQGKHTCLPRRAAQPSEPVVLGNSTSLKNMAITAEEERARRADILIKEVFTQLCKLSITFDRFLSDQQTRFIICLDASETKQCDVLAKLIYQIHSLVLTESTAPVAFVLEANFKVLLGDTVASAICPPYLVKPNNGVPEEAPRTLSDNLHLINSSIHLPIYLEAPLFTDVESEPHSKDLTPNSVNVSPNGFPHPGPAQSAKRTSGIMYTPRSIFGNRGSVAPFRGGPEKSSTQLLTGQLSHSNSTLSRSVE-----------------TCQGIAGLFLRDHELADWNGKMMKQLISATSFTSRFLRLCNLEVPIEEMILWITLVEYWPYHIAWLVVYLEGLIEGKSYGTGKPISNQTGINLIDCFQEVKARVAASID---QLEPLVEDDRDLERLEGYLRAKASRPISIGNLKKLISCVLIHNPFLRHCIRAQILNPDRRSASEDRRGGHLGPFSPALTTLV---EGQVDSFRKTNQNI--GSSGPYVMNR----SNSGKGRLSIPLGSTDAQGVHFFPCNADLLTSAEQTPRISYEGRTKEIDQHLLLSQYTVSDVCNLIDNIREIDPEHRYIYQTNVRDHNVNGLVICRTDSERLKHEIGMNQSDWQAFCRSVQYLHGQES 961
BLAST of Kinase D-interacting substrate vs. TrEMBL
Match: A0A4E0RMV2 (Uncharacterized protein OS=Fasciola hepatica OX=6192 GN=D915_001102 PE=4 SV=1) HSP 1 Score: 266.544 bits (680), Expect = 2.135e-68 Identity = 248/972 (25.51%), Postives = 442/972 (45.47%), Query Frame = 2
Query: 296 SSSDATILSGDMNKNNVEDFIGLNYFNYPLDHCGSNIRAQKTVTYIQALADMLVCPNTNFPISIGLFSRVGAGIRTTHLEKLSDELISLTKINNQKTLQPSLLLFMFPXXXXXXXXXXXXXXTNWIVGVSLGASLSLMSFLLIEIIIFGSNHKRWGKALRAARWLASFTQSLQLFLDILFRYPHENELTTKPLSIIITHHNLSNYSSASHMTLSIVEKLWKAVEKKYSCLLSRLQISCK-NPESSTKFKNICCIPIFVLVIIFFSAIIINATLFTIYNKNPMDIFVNKGLLPLFVIMTCIVFLCIFGTLPLVCRTITFLVRSPSRQLK----QFMESE--------------------------------------NIDFQA-------SDTLVKNELLKLCQIISIFEAYLNSEKIRMFVFIDAADTESREKLANSIYLVNGLLQMSSNAPIALILAADFQTLNTEWXXXXXXXXXXXXXXXXIFQE----ISDNFHLIHSTIHLPIYME-----------EGIDFNRNS---------------SGKKK----YASNGMFCIRG------GNSFESLNMVTTGTKQNSVPVSTRKTSIYXXXXXXXXXXXXXKGASKSIQQLFLTRGDLSDINNKMIKQLTAAAAFIGRLLRINLIEFKLENLISWLSISINWPYQTALMALFLE-----------EQCANLTPKHTLLQIHEQVLSKIGSKDSNPAEIELLARDDRDFGKLDAFIKKDVGATLTFSILKYFVSWSPFMNPYLRYVLRDYLTNKNLAQT----RGSRNQLTSPPLTSMALSDPSDVPMDEDLPQPIISPSTGSGLVNQITSWSKSSIGNLAHK-GDRPKTPIAIKRCMVKL--------KDGYPG--KDLSQ---LDKYEIGILMCQIEGLSSEENIEK--YKEKLIQLNVTGLVLSVCDMDQLQNEMEMNFGDWQLFKTLIYHLRSVEA 2848
S T S +N+++ + L F+ PLD ++++ T T I+ALADM C TNFP++ G+F+ +L +L+ EL ++++++ L+ S+LLF+FP+F+ +G +VG+ T W VG+S+G + + III GS A R + +A F + LQL L++ F YP + T P+ I+ +H L ++ + S+++K+W + + + + RLQ + + +P + +FK +CC+P F++V+ F + + + Y+ + KG + +FVI + I+FL +FG++P + R I L +P + L+ F+ E N+ A +D L+K +LC++ F+ +L+ ++ R + +DA++T+ + LA IY ++ L+ S AP+A +L A+F+ L + +S+ P + + + ++ +SDN HLI+S+IHLPIY+E + D NS S K+ Y +F RG G +S + TG +S +R + I +FL +L+D N KM+KQL +A +F R LR+ +E +E +I W+++ WPY A + ++LE + +N T L+ ++V +++ + ++E L DDRD +L+ +++ ++ LK +S NP+LR+ +R + N + RG SP LT++ V Q I S+G +N+ S S G L+ G + C L + Y G K++ Q L +Y + + I+ + E K Y+ + NV+GLV+ D ++L++E+ MN DWQ F + +L E+
Sbjct: 24 SKQPVTPSSTQSKRNDLDTLLELGAFHCPLDLITADVKNHGTNT-IRALADMFTCRLTNFPVTTGIFTTQND---KEYLSRLASELRNISRLSGPVALRFSVLLFLFPLFVSTFLGYLVGMLTTWQVGLSIGHVGMFLQITFLVIIITGSRVCHNHIATRISHSVAQFLRWLQLILNVCFCYPTQQIDTKAPVKTIVVYHRLWPSTTPNVALFSMIDKMWTGLIRMHGEISIRLQRASRASPLPNRRFKRVCCLPAFLVVLAFCVSFMTETGMIRYYSGEFTAMLTEKGTIVIFVISSSILFLGLFGSIPSLYRLIRSLTINPVQPLRTALADFLSDEANEEEYNHLRPQGKHTCLPRRVAQPSEPVVLGNSTSLKNMAITAEEERARRADILIKEVFTQLCKLSITFDRFLSDQQTRFIICLDASETKQCDVLAKLIYQIHSLVLTESTAPVAFVLEANFKVLLGDTVASAICPPYLGTPNTGVPEDAPRTLSDNLHLINSSIHLPIYLEAPLFTDVESEPQSKDLTPNSVNVSPNGFPHPAPAQSAKRTSGIMYTPRSIFGNRGSVAPFRGGPEKSSTQLLTGQLSHSNSTLSRSVE-----------------TCQGIAGIFLRDHELADWNGKMMKQLISATSFTSRFLRLCNLEVPIEEMILWITLVEYWPYHIAWLVVYLEGLVEGKSYGMGKPVSNQT-GINLIDCFQEVKARVAASID---QLEPLVEDDRDLERLEGYLRAKASRPISIGNLKKLISCVLIHNPFLRHCIRAQILNPDRRSASEDRRGGHLGPFSPALTTLV---EGQVDSFRKTNQNI--GSSGPYAMNR----SNSGKGRLSIPLGPTDAQGVHFFPCNADLLTSAEQTPRISYEGHTKEIGQHLLLSQYTVSDVCNLIDNIREIEPEHKYIYQTNVRDHNVSGLVICRTDSERLKHEIGMNQSDWQAFCRSVQYLHGQES 961
BLAST of Kinase D-interacting substrate vs. TrEMBL
Match: A0A4S2LEA8 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_008201 PE=4 SV=1) HSP 1 Score: 248.054 bits (632), Expect = 1.002e-62 Identity = 248/1004 (24.70%), Postives = 447/1004 (44.52%), Query Frame = 2
Query: 329 MNKNNVEDFIGLNYFNYPLDHCGSNIRAQKTVTYIQALADMLVCPNTNFPISIGLFSRVGAGIRTTHLEKLSDELISLTKINNQKTLQPSLLLFMFPXXXXXXXXXXXXXXTNWIVGVSLGASLSLMSFLLIEIIIFGSNHKRWGKALRAARWLASFTQSLQLFLDILFRYPHENELTTKPLSIIITHHNLSNYSSASHMTLSIVEKLWKAVEKKYSCLLSRLQISCKNP-ESSTKFKNICCIPIFVLVIIFFSAIIINATLFTIYNKNPMDIFVNKG-LLPLFVIMTCIVFLCIFGTLPLVCRTITFLVRSPSRQLK----QFMESENI--------------------------DFQ-------------ASDTLVKN-------------------ELLKLCQIISIFEAYLNSEKIRMFVFIDAADTESREKLANSIYLVNGLLQMSSNAPIALILAADFQTLNTE-------WXXXXXXXXXXXXXXXXIFQEISDNFHLIHSTIHLPIYME-------EGIDFNRNSSGKKKYASNGMFCIRGGNSFESLNMVT----TGTKQNSVPVSTRKTSIYXXXX---XXXXXXXXXKGASKSI------QQLFLTRGD-----------------------LSDINNKMIKQLTAAAAFIGRLLRINLIEFKLENLISWLSISINWPYQTALMALFLEEQCAN-----LTPKHTLLQIHEQVLSKIGSKDSNPAE-IELLARDDRDFGKLDAFIKKDVGATLTFSILKYFVSWSPFMNPYLRYVLRDYLTNKNLAQTRGSRNQLTSPPLTSMALSDPSDVPM---DEDLPQPI---------ISPSTGSGLVNQITSWSKSSI-----GNLAHKGDRP-KTPIAIKRCMVKLKDGYPGKDLSQ-LDKYEIGILMCQIEGLSSEENIEK---YKEKLIQLNVTGLVLSVCDMDQLQNEMEMNFGDWQLFKTLIYHLRSVEAFKSDDLS-IVGDPFVD--PNSF 2905
++KN++E + L ++YPL+ ++R T ++ALADM C TNFP+++G+F++ + HL L+ EL ++ + + TL+ + LLF+FP+F+ +G + G+ ++W VG+S+G M + + +II GS + A+R + +A F L+L L++ F YP + T + I+ +H L S+ + SI++K+W + + + + RL + P E ++K +CC+P F++VI+F+ I+ + N + + +++ ++ FV M+ I+FL I G+LP + R I L SP + L+ F+ E DFQ + T +KN E KLC++ F+++L ++ R + +DA++T R+ LA IY ++ L+ NAP+A +L ADF+ L E +++ S + + + ++DN HL+ ++I LPIY++ E + + +++ N + G S S N VT G + P ++ Y N + + KG+ KSI ++L TR L+D N K +QL AA AF R LR+ + +++++ W+S+ WPY A + L +EE + P L+ + E L ++ S E +E L DDR +L+++IK + +L LK S NPYLR +R L T + PP+ S +P+ ++D + + SP+ GL+ + +S + I N D+ P+ + + G L++ L ++ + I +C + +++ Y+ + +++G L D+++++ E+ M DWQ F ++ HLRS KS S I G+ D PN+
Sbjct: 44 LDKNDLETVLELGVYHYPLELHTPDVRNHGANT-VRALADMFTCRMTNFPLTVGIFNQQN---KNDHLSDLASELHNINSLMDPTTLKFNPLLFLFPLFISTTLGYLFGMVSSWQVGLSIGHLAMFMQIIFLVVIITGSRIFQKNFAIRLSHKVAEFLLWLRLVLNVCFCYPTQRLDKTSSVRTIVVYHRLFPSSTPTVALFSILDKMWTGLTRLHGEIPIRLLRASPLPREPRYRYKRVCCLPAFLVVIVFWIVFIVGTAVLRD-NSGQLKVLLSRATIMITFVAMSSIMFLTILGSLPSIYRLIRGLAVSPIKPLRTALADFLSDETTSSAAGTAVGLASAGEDVDQLLNKIKPDFQKNRRGTVNEGVQLGTSTSLKNLAISAEEERARRADIRIKEEFGKLCRLSLTFDSFLGEQQTRFIMCLDASETNQRDVLAKLIYQIHSLVLTDVNAPVAFVLEADFKVLLGEVAAGAIFHPTNAYPAASSAQLARVVSKTLNDNLHLVRTSIQLPIYLDTPVCSDVEHCEAPCTTHPRQQPQLNSIITSPNGYSQTSPNSVTPFCEAGNQPGFAPFRRSTSTTYTPRALFTNRTSVPMSPKGSGKSIGLPLKVKKLSFTRSSSTVSRAAECSAGIASLFIQDDNPLADWNKKTSRQLVAAVAFTSRFLRLCNLNIPMDDVVFWISLVHYWPYHVAWLVLNIEEHTRGELPRTVPPNDALVNL-EACLHQVKSSIGETLELLEPLMADDRSLERLESYIK--LNPSLPLMTLKKLTSCILTHNPYLRQCIR-ALAGSASTSTYNKDEIVFYPPVG-------SGLPVLIEEQDTGEKMGRSKSRGDGRSPTNMFGLMERTSSNKSTPIFFREMTNSTDSADKVFANPMWEQTGAIGPSSNQTGPQLAKALHQFSV-IDVCNLLANLPNLPLDRLRRYQNNVRHHHISGAALLASDLEKVRRELAMECEDWQNFSAILAHLRS--RVKSHQTSGIFGNGLADSIPNTL 1028
BLAST of Kinase D-interacting substrate vs. TrEMBL
Match: A0A419Q125 (Uncharacterized protein OS=Clonorchis sinensis OX=79923 GN=CSKR_4307s PE=4 SV=1) HSP 1 Score: 239.58 bits (610), Expect = 4.871e-60 Identity = 244/991 (24.62%), Postives = 446/991 (45.01%), Query Frame = 2
Query: 329 MNKNNVEDFIGLNYFNYPLDHCGSNIRAQKTVTYIQALADMLVCPNTNFPISIGLFSRVGAGIRTTHLEKLSDELISLTKINNQKTLQPSLLLFMFPXXXXXXXXXXXXXXTNWIVGVSLGASLSLMSFLLIEIIIFGSNHKRWGKALRAARWLASFTQSLQLFLDILFRYPHENELTTKPLSIIITHHNLSNYSSASHMTLSIVEKLWKAVEKKYSCLLSRLQISCKNP-ESSTKFKNICCIPIFVLVIIFFSAIIINATLFTIYNKNPMDIFVNKGLLPLFVIMTCIVFLCIFGTLPLVCRTITFLVRSPSRQLK------------------------------QFMESENIDFQ-------------ASDTLVKN-------------------ELLKLCQIISIFEAYLNSEKIRMFVFIDAADTESREKLANSIYLVNGLLQMSSNAPIALILAADFQTLNTE-------WXXXXXXXXXXXXXXXXIFQEISDNFHLIHSTIHLPIYME-------EGIDFNRNSSGKKKYASNGMFCIRGGNSFESLNMVTTGTK--------------------------QNSVPVSTRKTS---------IYXXXXXXXXXXXXXKGASKSIQQLFLTRGD-LSDINNKMIKQLTAAAAFIGRLLRINLIEFKLENLISWLSISINWPYQTALMALFLEEQCANLTPK----HTLLQIHEQVLSKIGSKDSNPAE-IELLARDDRDFGKLDAFIKKDVGATLTFSILKYFVSWSPFMNPYLRYVLRDYLTNKNLAQTRGSRNQLTSPPLTS--MALSDPSDVPMDEDLPQPII-----SPSTGSGLVNQITSWSKSSI-----GNLAHKGDRPKT-PIAIKRCMVKLKDGYPGKDLSQ-LDKYEIGILMC---QIEGLSSEENIEKYKEKLIQLNVTGLVLSVCDMDQLQNEMEMNFGDWQLFKTLIYHLRS-VEAFKSDDLSIVGDPFVD 2893
++KN++E + L ++YPL+ ++R T ++ALADM C T+FP+++G+F++ + HL L+ EL ++ + + TL+ + LLF+FP+F+ +G ++G+ ++W VG+S+G + + + +II GS + A+R + +A F L+L L++ F YP + + I+ +H L S+ + SI++K+W + + + + RL + P E ++K +CC+P F++VI+F+ A I+ + + + ++ FV M+ I+FL I G+LP + R I L SP + L+ Q + DFQ + T +KN E KLC++ F+++L ++ R + +DA++T R+ LA IY ++ L+ NAP+A +L ADF+ L E +++ S + + + ++DN HL+ ++I LPIY++ E + + +++ N + G S S N VT+ + + SVP+S + + LS S + S + S I LF+ + L+D N K KQL AA AF R LR+ ++ +++++ W+S+ WPY A + L +EE P+ + L E L ++ S E +E L DDR +L+++IK + +L LK S NPYL +R N +++ L PP+ S AL + D E + + SP+T GL+ + +S + I N D+ T P+ + + G L++ L ++ + I +C + + +Y+ + +++G L D+++++ E+ M DWQ F ++ HL+S V++ ++ D I G+ D
Sbjct: 36 LDKNDLETVLELGVYHYPLELHTPDVRNHGANT-VRALADMFTCRMTHFPLTVGIFNQQN---KNDHLSDLASELHNINSLMDPTTLKFNPLLFLFPLFISTTLGYLIGMVSSWQVGLSIGHLAMFIQIIFLVVIITGSRIFQKTFAIRLSHKVAEFLLWLRLVLNVCFCYPTQQLDKKSSVRTIVVYHRLFPSSTPTVALFSILDKMWTGLIRLHGEIPIRLLRASPVPREPRHRYKRVCCLPAFLVVIVFWIAFIVGTAVLRDNSGQLKMLLSRAAIMITFVAMSSIMFLTILGSLPSIYRLIRGLAVSPIKPLRTALADFLSDETSSSAAGTTVGLASAGEDVDQLLNKIKPDFQKNRRGTVNEGVQLGTSTSLKNLAISAEEERARRADIRIKEEFGKLCRLSLTFDSFLGEQQTRFIMCLDASETNQRDVLAKLIYQIHSLVLTDVNAPVAFVLEADFKVLLGEVAAGAIFHPTNAYPAASSAQLARVVSKTLNDNLHLVRTSIQLPIYLDTPVCSDVEHCEAPCITHPRQQPQLNSIITSPNGYSQTSPNYVTSFCEAENQPGFAPFRRSTDTTYTPRALFANRTSVPISPKSSGKSIGLPLKVKKLSFTRSSSTTSRAAECSAGIAGLFIQDDNPLADWNRKTSKQLVAAMAFTSRFLRLCNLDIPMDDVVFWISLVHYWPYHMAWLVLNIEEHTRGELPRTVSSNDALVNLEACLHQVKSSIGETLELLEPLMADDRSLERLESYIKSN--PSLPLMTLKKLTSCVLMHNPYLLQCIRALAGNASVS-IHTKDEILFYPPIGSGLPALIEEQDT--GEKIGRSKTRGDGRSPTTMYGLMERTSSNKSTPIFLREMTNSTDSADKVFTNPMWEQTGATEPSFNQTGPQLAKALHQFSV-IDVCNLLANLPNLPLDRLRRYQNNVRHHHISGAALLASDLEKVRRELAMECEDWQNFSAILAHLKSRVKSHQNSD--IFGNGLAD 1014
BLAST of Kinase D-interacting substrate vs. Ensembl Cavefish
Match: kidins220a (kinase D-interacting substrate of 220 kDa [Source:NCBI gene;Acc:103043425]) HSP 1 Score: 177.178 bits (448), Expect = 3.450e-44 Identity = 213/852 (25.00%), Postives = 378/852 (44.37%), Query Frame = 2
Query: 428 YIQALADMLVCPNTNFPISIGLFSRVGAGIRTTHLEKLSDELISLTKINNQKTLQPSLLLFMFPXXXXXXXXXXXXXXTNWIVGVSLGASLSLMSFLLIEIIIFGSNH--KRWGKALRAARWLASFTQSLQLFLDILFRYPHE-NELTTK--PLSIIITHHN-LSNYSSASHMTLSIVEKLWKAVEKKYSCLLSRLQISCKNPES-STKFKNICCIPIFVLVIIFFSAIIINATLFTIYNKNPMDIFVNKGLLPLFVIMTCIVFLCIFGTLPLVCRT----ITFLVRSPSRQLKQFMES-ENIDFQASDTLVKNELLKLCQIISIFEAYLNSEKIRMFVFIDAADTESREKLANSIYLVNGLLQMSSNAPIALILAADFQTLNTEWXXXXXXXXXXXXXXXXIFQEISDNFH-LIHSTIHLPIYMEEGIDFNRNSSGKKKYA----SNG------MFCIRGGNSFESLNMVTTGTKQNSVPVSTRKTSIYXXXXXXXXXXXXXKGASKSIQQLFLTRGDLSDINNKMIKQLTAAAAFIGRLLRINLIEFKLENLISWLSISINWPYQTALMALFLEEQCANLTPKHTLLQIHEQVLSKIGSKDSNPAEIELLARDDRDFGKLDAFIKKDVGATLTFSILKYFVSWSPFMNPYLRYVLRDYLTNKNLAQTRGSRN---QLTSPPLTSMAL--SDPSDVPMDEDLP-QPIISPSTGSGLVN-------------QITSWSKSSIGNLAHKGDRPKTPIAIKRCMVK-LKDGY--PGKDLSQLDKYEIGILMCQIEGLSSEENIEKYKEKLIQLNVTGLVLSVCDMDQLQNEMEMNFGDWQLFKTLIYHLRSVEA 2848
Y ALAD+L P PI +GL+++ G+G ++ L+KL DE+ + + LQ S L+ + + L V +I+G + + V++ SL + ++ ++ FG + W A + LA L+L L ++F P E E TTK P+ + T +N LS+ + M ++ L A E+++ + +RL KN ES K+K CC+P FVL + + ++ L I+ P +I VN + V M +V L + L CRT ++ S R+L S + + ++K E+ + ++ + + ++ R+ V ID D+ ++K+ + V L S P I A+D + + + +++ ++ + N H + + +HLP+++ +R S KK + +NG + F+S N + KQ S R+ + + ++ S + +L +T SDI+ + +++L + GRLLR N I F + L SW++++ WPY+T+ + LFLEE ++ + TL I+E++ I + ++E L D D + F+ LT ++ F+ + ++P LR ++ D + G L P + ++ P+ P P QP S SGL + + GN H RP + +A + + L G P LS L + + I+GL + +E+Y + + N+ G VL C++D+L+ EM MNFGDW LFK ++ R VE+
Sbjct: 451 YSSALADILSEPTMQPPICVGLYAQWGSG-KSFLLKKLEDEMKTFAGQQVEPLLQFSWLVVVLSLLLCGSVALILGFTVDTKLAVAVALSLLALLYIFFVLVYFGGRKGGESWSWAWNISTRLARHIGYLELLLKLMFVNPPELPEQTTKALPVRFLFTDYNRLSSVGGETSMA-EMIATLSDACEREFGFMATRLFRVFKNDESQGKKWKKTCCVPSFVLFALTLACLVTGMALLAIFKVEPSNITVNA----VLVAMASVVGLALL----LNCRTWWQVADSVLHSQRRRLHSAANSMHKLKSEGFMKVLKCEVELMAKMAKTIDGFTQNQ-TRLVVIIDGLDSCEQDKVLQMLDTVRVLF---SKGPFISIFASDPHII----------IKAINQNLNSVLRDSNINGHDYMRNIVHLPVFLN-----SRGLSTAKKLSMAAPTNGDLLSAEAWHEEMDRKFQSQNSLGEQAKQGSKTALNRRDTY----RRRQMQRTVTRQMSFDLTKLLVTEDWFSDISPQTMRRLLNIVSVTGRLLRANQIIFNWDRLASWINLTEEWPYRTSWLILFLEENDI-VSEQVTLKTIYERISKNIPTTK----DVEPLLEIDGDIRSFEVFLSSRT-PVLTAHDVEMFLPCTVNLDPKLREIIADVRDAREQMHVGGVSYPTLPLQEAPARTHSVFGQAPAACPPTASFPTQP--HSSYFSGLTGPQHPFYNRPYFPQHVYHVPRHYPGNPLHAPSRPPSKLAFHKDLSSGLGSGAAIPPGPLSSLSIEGVCERLRHIDGL-DDSMLEQYTTTVKKANINGRVLCQCNLDELRKEMNMNFGDWHLFKAVVLEQRQVES 1260
BLAST of Kinase D-interacting substrate vs. Ensembl Cavefish
Match: kidins220b (kinase D interacting substrate 220 [Source:NCBI gene;Acc:103040274]) HSP 1 Score: 176.022 bits (445), Expect = 8.861e-44 Identity = 202/844 (23.93%), Postives = 379/844 (44.91%), Query Frame = 2
Query: 428 YIQALADMLVCPNTNFPISIGLFSRVGAGIRTTHLEKLSDELISLTKINNQKTLQPSLLLFMFPXXXXXXXXXXXXXXTNWIVGVSLGASLSLMSFLLIEIIIFGSNHK--RWGKALRAARWLASFTQSLQLFLDILFRYPHE-NELTTK--PLSIIITHHN-LSNYSSASHMTLSIVEKLWKAVEKKYSCLLSRL--QISCKNPESSTKFKNICCIPIFVLVIIFFSAIIINATLFTIYNKNPMDIFVNKGLLPLFVIMTCIVFLCIFGTLPLVCRTITFLVRSP-SRQLKQFMESEN----IDFQASDTLVKNELLKLCQIISIFEAYLNSEKIRMFVFIDAADTESREKLANSIYLVNGLLQMSSNAPIALILAADFQTLNTEWXXXXXXXXXXXXXXXXIFQEISDNFH-LIHSTIHLPIYMEEGIDFNRNSSGKKKYASNGMFCIRGGNSFESLNMVTTGTKQNSVPVSTR---KTSI--YXXXXXXXXXXXXXKGASKSIQQLFLTRGDLSDINNKMIKQLTAAAAFIGRLLRINLIEFKLENLISWLSISINWPYQTALMALFLEEQCANLTPKHTLLQIHEQVLSKIGSKDSNPAEIELLARDDRDFGKLDAFIKKDVGATLTFSILKYFVSWSPFMNPYLRYVLRDYLTNKNLAQTRGSRNQLTSPPLTSMALSDPSDVPMDEDLPQPI---------ISPSTG-------SGLVNQITSWSKSSIGNLAHK-GDRPKT-PIAIKRCMVKLKDGYPGKDLSQLDKYEIGILMCQIEGLSSEENIEKYKEKLIQLNVTGLVLSVCDMDQLQNEMEMNFGDWQLFKTLIYHLRSVEA 2848
Y ALAD+L P PI +GL+++ G+G ++ L+KL DE+ + + Q S L+ + L V +++G + + +++ SL + ++ ++ FGS + W A + LA L+L L ++F P E E TT+ P+ + T +N LS+ + M ++ L A E+++ + SRL ++ + K+K CCIP FV+ + +I L I+ + + VN + V M +V L + L CRT + S + Q K+ + N + + ++K+E+ + ++ +++ ++ R+ V ID D+ ++K+ + V L S P I A+D + + + +++ ++ + N H + + +HLP+++ +R S KK + GNS + Q+S+ + + KT++ + S ++ S + +L +T SDI+ + +++L + GRLLR N I F + L SW++++ WPY+T+ + L+LEE + + TL I+E++ I + ++E L D D + F+ L ++ F+ + ++P LR ++ D R +R Q+ +T L P+ E P+P SP+ G+ Q S S + H +R K P+ K+ + G P LS ++ + + I+G+ + + +Y + + NV G VLS C++D+L+ EM MNFGDWQLF+ + +R +E+
Sbjct: 451 YSSALADILSEPTMQPPICVGLYAQWGSG-KSFLLKKLEDEMKTFAGQQIEPLFQFSWLVVFLSLMLCGSVALLLGFTVDPKLAIAVSLSLLALLYVFFVVVYFGSRREGESWNWAWVLSTRLARHIGYLELLLKLMFVNPPELPEQTTRALPVRFLFTDYNRLSSVGGETSMA-EMIATLSDACEREFGFMASRLFRVFKTEDTQGKKKWKKTCCIPSFVIFLFILVCLITGMALLAIFKVDGENQTVNA----ILVAMVSVVGLAVL----LNCRTWWQVTDSVLNSQRKRLHSAANKMHKLKSEGFMKVLKSEVELMAKMAKTIDSFTQNQ-TRLVVIIDGLDSCEQDKVLQMLDTVRVLF---SKGPFISIFASDPHII----------IKAINQNLNSVLRDSNINGHDYMRNIVHLPVFLN-----SRGLSSAKKMCAPAPANGETGNSEGWHEELDRKLSQHSLSEAAKLGSKTALNRRDTYRRRQMQRSVTRQMSFDLTKLLVTEDWFSDISPQTMRRLLNIVSVTGRLLRANQISFNWDRLASWINLTEQWPYRTSWLILYLEE-TDGIPDQATLKTIYERISKNIPTT----KDVEPLLEIDGDVRSFEVFLSSRT-PVLAARDIRTFLPCTVNLDPKLREIIAD---------VRAAREQMNMGGVTYPTL------PLHEAGPRPTSIYSQQSSACSPTASYNGPYNPPGVSPQPHSAYYSGMAGPQHPFYNRFKVVPLKTKQGTASVVSGAPSVLLSSMNTDMVCERLKVIDGMD-QAMLAQYTATIKKANVNGRVLSQCNLDELKKEMSMNFGDWQLFRGTVMEMRHIES 1243
BLAST of Kinase D-interacting substrate vs. Ensembl Cavefish
Match: kidins220a (kinase D-interacting substrate of 220 kDa [Source:NCBI gene;Acc:103043425]) HSP 1 Score: 176.022 bits (445), Expect = 9.383e-44 Identity = 213/862 (24.71%), Postives = 380/862 (44.08%), Query Frame = 2
Query: 428 YIQALADMLVCPNTNFPISIGLFSRVGAGIRTTHLEKLSDELISLTKINNQKTLQPSLLLFMFPXXXXXXXXXXXXXXTNWIVGVSLGASLSLMSFLLIEIIIFGSNH--KRWGKALRAARWLASFTQSLQLFLDILFRYPHE-NELTTK--PLSIIITHHN-LSNYSSASHMTLSIVEKLWKAVEKKYSCLLSRLQISCKNPES-STKFKNICCIPIFVLVIIFFSAIIINATLFTIYNKNPMDIFVNKGLLPLFVIMTCIVFLCIFGTLPLVCRT----ITFLVRSPSRQLKQFMES-ENIDFQASDTLVKNELLKLCQIISIFEAYLNSEKIRMFVFIDAADTESREKLANSIYLVNGLLQMSSNAPIALILAADFQTLNTEWXXXXXXXXXXXXXXXXIFQEISDNFH-LIHSTIHLPIYMEEGIDFNRNSSGKKKYA----SNG------MFCIRGGNSFESLNMVTTGTKQNSVPVSTRKTSIYXXXXXXXXXXXXXKGASKSIQQLFLTRGDLSDINNKMIKQLTAAAAFIGRLLRINLIEFKLENLISWLSISINWPYQTALMALFLEEQCANLTPKHTLLQIHEQVLSKIGSKDSNPAEIELLARDDRDFGKLDAFIKKDVGATLTFSILKYFVSWSPFMNPYLRYVLRDYLTNKNLAQTRGSRN---QLTSPPLTSMAL--SDPSDVPMDEDLP-QPIISPSTGSGLVN-------------QITSWSKSSIGNLAHKGDRPKTPIAIKRCMV---------KLKDG----YPGKDLSQLDKYEIGILMCQIEGLSSEENIEKYKEKLIQLNVTGLVLSVCDMDQLQNEMEMNFGDWQLFKTLIYHLRSVEA 2848
Y ALAD+L P PI +GL+++ G+G ++ L+KL DE+ + + LQ S L+ + + L V +I+G + + V++ SL + ++ ++ FG + W A + LA L+L L ++F P E E TTK P+ + T +N LS+ + M ++ L A E+++ + +RL KN ES K+K CC+P FVL + + ++ L I+ P +I VN + V M +V L + L CRT ++ S R+L S + + ++K E+ + ++ + + ++ R+ V ID D+ ++K+ + V L S P I A+D + + + +++ ++ + N H + + +HLP+++ +R S KK + +NG + F+S N + KQ S R+ + + ++ S + +L +T SDI+ + +++L + GRLLR N I F + L SW++++ WPY+T+ + LFLEE ++ + TL I+E++ I + ++E L D D + F+ LT ++ F+ + ++P LR ++ D + G L P + ++ P+ P P QP S SGL + + GN H RP + +A + + ++K G P LS L + + I+GL + +E+Y + + N+ G VL C++D+L+ EM MNFGDW LFK ++ R VE+
Sbjct: 451 YSSALADILSEPTMQPPICVGLYAQWGSG-KSFLLKKLEDEMKTFAGQQVEPLLQFSWLVVVLSLLLCGSVALILGFTVDTKLAVAVALSLLALLYIFFVLVYFGGRKGGESWSWAWNISTRLARHIGYLELLLKLMFVNPPELPEQTTKALPVRFLFTDYNRLSSVGGETSMA-EMIATLSDACEREFGFMATRLFRVFKNDESQGKKWKKTCCVPSFVLFALTLACLVTGMALLAIFKVEPSNITVNA----VLVAMASVVGLALL----LNCRTWWQVADSVLHSQRRRLHSAANSMHKLKSEGFMKVLKCEVELMAKMAKTIDGFTQNQ-TRLVVIIDGLDSCEQDKVLQMLDTVRVLF---SKGPFISIFASDPHII----------IKAINQNLNSVLRDSNINGHDYMRNIVHLPVFLN-----SRGLSTAKKLSMAAPTNGDLLSAEAWHEEMDRKFQSQNSLGEQAKQGSKTALNRRDTY----RRRQMQRTVTRQMSFDLTKLLVTEDWFSDISPQTMRRLLNIVSVTGRLLRANQIIFNWDRLASWINLTEEWPYRTSWLILFLEENDI-VSEQVTLKTIYERISKNIPTTK----DVEPLLEIDGDIRSFEVFLSSRT-PVLTAHDVEMFLPCTVNLDPKLREIIADVRDAREQMHVGGVSYPTLPLQEAPARTHSVFGQAPAACPPTASFPTQP--HSSYFSGLTGPQHPFYNRPYFPQHVYHVPRHYPGNPLHAPSRPPSKLAFHKDLSSGLTKSQPPRVKQGSGAAIPPGPLSSLSIEGVCERLRHIDGL-DDSMLEQYTTTVKKANINGRVLCQCNLDELRKEMNMNFGDWHLFKAVVLEQRQVES 1270
BLAST of Kinase D-interacting substrate vs. Ensembl Cavefish
Match: kidins220b (kinase D interacting substrate 220 [Source:NCBI gene;Acc:103040274]) HSP 1 Score: 174.481 bits (441), Expect = 2.445e-43 Identity = 198/835 (23.71%), Postives = 374/835 (44.79%), Query Frame = 2
Query: 428 YIQALADMLVCPNTNFPISIGLFSRVGAGIRTTHLEKLSDELISLTKINNQKTLQPSLLLFMFPXXXXXXXXXXXXXXTNWIVGVSLGASLSLMSFLLIEIIIFGSNHK--RWGKALRAARWLASFTQSLQLFLDILFRYPHE-NELTTK--PLSIIITHHN-LSNYSSASHMTLSIVEKLWKAVEKKYSCLLSRL--QISCKNPESSTKFKNICCIPIFVLVIIFFSAIIINATLFTIYNKNPMDIFVNKGLLPLFVIMTCIVFLCIFGTLPLVCRTITFLVRSP-SRQLKQFMESEN----IDFQASDTLVKNELLKLCQIISIFEAYLNSEKIRMFVFIDAADTESREKLANSIYLVNGLLQMSSNAPIALILAADFQTLNTEWXXXXXXXXXXXXXXXXIFQEISDNFH-LIHSTIHLPIYMEEGIDFNRNSSGKKKYASNGMFCIRGGNSFESLNMVTTGTKQNSVPVSTR---KTSI--YXXXXXXXXXXXXXKGASKSIQQLFLTRGDLSDINNKMIKQLTAAAAFIGRLLRINLIEFKLENLISWLSISINWPYQTALMALFLEEQCANLTPKHTLLQIHEQVLSKIGSKDSNPAEIELLARDDRDFGKLDAFIKKDVGATLTFSILKYFVSWSPFMNPYLRYVLRDYLTNKNLAQTRGSRNQLTSPPLTSMALSDPSDVPMDEDLPQPIISPSTGSGLVNQITSWSKS----SIGNLAHKG-----DRPKTPIAIKRCMVKLKDGYPGKDLSQLDKYEIGILMCQIEGLSSEENIEKYKEKLIQLNVTGLVLSVCDMDQLQNEMEMNFGDWQLFKTLIYHLRSVEA 2848
Y ALAD+L P PI +GL+++ G+G ++ L+KL DE+ + + Q S L+ + L V +++G + + +++ SL + ++ ++ FGS + W A + LA L+L L ++F P E E TT+ P+ + T +N LS+ + M ++ L A E+++ + SRL ++ + K+K CCIP FV+ + +I L I+ + + VN + V M +V L + L CRT + S + Q K+ + N + + ++K+E+ + ++ +++ ++ R+ V ID D+ ++K+ + V L S P I A+D + + + +++ ++ + N H + + +HLP+++ +R S KK + GNS + Q+S+ + + KT++ + S ++ S + +L +T SDI+ + +++L + GRLLR N I F + L SW++++ WPY+T+ + L+LEE + + TL I+E++ I + ++E L D D + F+ L ++ F+ + ++P LR ++ D R +R Q+ +T L P+ E P+P S S + S++ + H P+ P R + G P LS ++ + + I+G+ + + +Y + + NV G VLS C++D+L+ EM MNFGDWQLF+ + +R +E+
Sbjct: 451 YSSALADILSEPTMQPPICVGLYAQWGSG-KSFLLKKLEDEMKTFAGQQIEPLFQFSWLVVFLSLMLCGSVALLLGFTVDPKLAIAVSLSLLALLYVFFVVVYFGSRREGESWNWAWVLSTRLARHIGYLELLLKLMFVNPPELPEQTTRALPVRFLFTDYNRLSSVGGETSMA-EMIATLSDACEREFGFMASRLFRVFKTEDTQGKKKWKKTCCIPSFVIFLFILVCLITGMALLAIFKVDGENQTVNA----ILVAMVSVVGLAVL----LNCRTWWQVTDSVLNSQRKRLHSAANKMHKLKSEGFMKVLKSEVELMAKMAKTIDSFTQNQ-TRLVVIIDGLDSCEQDKVLQMLDTVRVLF---SKGPFISIFASDPHII----------IKAINQNLNSVLRDSNINGHDYMRNIVHLPVFLN-----SRGLSSAKKMCAPAPANGETGNSEGWHEELDRKLSQHSLSEAAKLGSKTALNRRDTYRRRQMQRSVTRQMSFDLTKLLVTEDWFSDISPQTMRRLLNIVSVTGRLLRANQISFNWDRLASWINLTEQWPYRTSWLILYLEE-TDGIPDQATLKTIYERISKNIPTT----KDVEPLLEIDGDVRSFEVFLSSRT-PVLAARDIRTFLPCTVNLDPKLREIIAD---------VRAAREQMNMGGVTYPTL------PLHEAGPRPTSIYSQQSSACSPTASYNGPYNPPGVSPQPHSAYYSGMAGPQHPF-YNRGTASVVSGAPSVLLSSMNTDMVCERLKVIDGMD-QAMLAQYTATIKKANVNGRVLSQCNLDELKKEMSMNFGDWQLFRGTVMEMRHIES 1233
BLAST of Kinase D-interacting substrate vs. Ensembl Cavefish
Match: kidins220a (kinase D-interacting substrate of 220 kDa [Source:NCBI gene;Acc:103043425]) HSP 1 Score: 173.711 bits (439), Expect = 3.642e-43 Identity = 211/844 (25.00%), Postives = 377/844 (44.67%), Query Frame = 2
Query: 428 YIQALADMLVCPNTNFPISIGLFSRVGAGIRTTHLEKLSDELISLTKINNQKTLQPSLLLFMFPXXXXXXXXXXXXXXTNWIVGVSLGASLSLMSFLLIEIIIFGSNH--KRWGKALRAARWLASFTQSLQLFLDILFRYPHE-NELTTK--PLSIIITHHN-LSNYSSASHMTLSIVEKLWKAVEKKYSCLLSRLQISCKNPES-STKFKNICCIPIFVLVIIFFSAIIINATLFTIYNKNPMDIFVNKGLLPLFVIMTCIVFLCIFGTLPLVCRT----ITFLVRSPSRQLKQFMES-ENIDFQASDTLVKNELLKLCQIISIFEAYLNSEKIRMFVFIDAADTESREKLANSIYLVNGLLQMSSNAPIALILAADFQTLNTEWXXXXXXXXXXXXXXXXIFQEISDNFH-LIHSTIHLPIYMEEGIDFNRNSSGKKKYA----SNG------MFCIRGGNSFESLNMVTTGTKQNSVPVSTRKTSIYXXXXXXXXXXXXXKGASKSIQQLFLTRGDLSDINNKMIKQLTAAAAFIGRLLRINLIEFKLENLISWLSISINWPYQTALMALFLEEQCANLTPKHTLLQIHEQVLSKIGSKDSNPAEIELLARDDRDFGKLDAFIKKDVGATLTFSILKYFVSWSPFMNPYLRYVLRDYLTNKNLAQTRGSRNQLTSPPLTSMALSDPSDVPMDEDLPQ--------PIISPSTGSGLVNQITSWSKSSIGNLAHKGDRPKTPI--AIKRCMVKLKDG----YPGKDLSQLDKYEIGILMCQIEGLSSEENIEKYKEKLIQLNVTGLVLSVCDMDQLQNEMEMNFGDWQLFKTLIYHLRSVEA 2848
Y ALAD+L P PI +GL+++ G+G ++ L+KL DE+ + + LQ S L+ + + L V +I+G + + V++ SL + ++ ++ FG + W A + LA L+L L ++F P E E TTK P+ + T +N LS+ + M ++ L A E+++ + +RL KN ES K+K CC+P FVL + + ++ L I+ P +I VN + V M +V L + L CRT ++ S R+L S + + ++K E+ + ++ + + ++ R+ V ID D+ ++K+ + V L S P I A+D + + + +++ ++ + N H + + +HLP+++ +R S KK + +NG + F+S N + KQ S R+ + + ++ S + +L +T SDI+ + +++L + GRLLR N I F + L SW++++ WPY+T+ + LFLEE ++ + TL I+E++ I + ++E L D D + F+ LT ++ F+ + ++P LR ++ D R +R Q+ +S P+ +P+ E + P P T S +S+ G P+ P K ++K G P LS L + + I+GL + +E+Y + + N+ G VL C++D+L+ EM MNFGDW LFK ++ R VE+
Sbjct: 451 YSSALADILSEPTMQPPICVGLYAQWGSG-KSFLLKKLEDEMKTFAGQQVEPLLQFSWLVVVLSLLLCGSVALILGFTVDTKLAVAVALSLLALLYIFFVLVYFGGRKGGESWSWAWNISTRLARHIGYLELLLKLMFVNPPELPEQTTKALPVRFLFTDYNRLSSVGGETSMA-EMIATLSDACEREFGFMATRLFRVFKNDESQGKKWKKTCCVPSFVLFALTLACLVTGMALLAIFKVEPSNITVNA----VLVAMASVVGLALL----LNCRTWWQVADSVLHSQRRRLHSAANSMHKLKSEGFMKVLKCEVELMAKMAKTIDGFTQNQ-TRLVVIIDGLDSCEQDKVLQMLDTVRVLF---SKGPFISIFASDPHII----------IKAINQNLNSVLRDSNINGHDYMRNIVHLPVFLN-----SRGLSTAKKLSMAAPTNGDLLSAEAWHEEMDRKFQSQNSLGEQAKQGSKTALNRRDTY----RRRQMQRTVTRQMSFDLTKLLVTEDWFSDISPQTMRRLLNIVSVTGRLLRANQIIFNWDRLASWINLTEEWPYRTSWLILFLEENDI-VSEQVTLKTIYERISKNIPTTK----DVEPLLEIDGDIRSFEVFLSSRT-PVLTAHDVEMFLPCTVNLDPKLREIIAD---------VRDAREQMHVG-----GVSYPT-LPLQEAPARTHSVFGQAPAACPPTASFPTQPHSSYFSGLTG--------PQHPFYNRTKSQPPRVKQGSGAAIPPGPLSSLSIEGVCERLRHIDGL-DDSMLEQYTTTVKKANINGRVLCQCNLDELRKEMNMNFGDWHLFKAVVLEQRQVES 1231
BLAST of Kinase D-interacting substrate vs. Ensembl Nematostella
Match: EDO35353 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SLT1]) HSP 1 Score: 51.9878 bits (123), Expect = 1.226e-6 Identity = 55/261 (21.07%), Postives = 112/261 (42.91%), Query Frame = 2
Query: 785 WLASFTQSLQLFLDILFRYPHENELTTKPLSIIITHHNLSNYSSASHMTLSIV---EKLWKAVEKKYSCLLSRLQISCKNPESSTKFKNI-----------CCIPIFVLVIIFFSAIIINATLFTIYNKNPMDIFVNKGLLPLFVIMTCIVFLCIFGTLPLVCRTITFLVRSPSRQLKQFMESENIDFQASDTLVKNELLKLCQIISIFEAYLNSEKIRMFVFIDAADTESREKLANSIYLVNGLLQMSSNAPIALILAAD 1525
++A+ +L+L ++F P L + + S + + S+V E L VE ++ ++RL + P S ++++I CCIP F++ + +++A+LFT+Y+ + I L + IV + + LP +C + LV S +++ + + +K E+ L +++ + + + R+ V ID D + K+ + VN LL +AP +++A D
Sbjct: 76 YVATSVTTLKLLSQLIFCIPPCPSAMKPALPVRFLFTDFSKLTCSGSEAASLVGMIETLCDVVEAEFGFFVTRLYRVFRAP--SAEYEDIGYTEARWKRCCCCIPTFIIFLFIIVCGLMSASLFTVYDTSGGSIVTG-----LEISTASIVGVAVLVHLPTLCSILYSLVFSQKKRISVVATQLGLKEEGFIHALKQEVELLTDLVNCVDGF-TRHQTRIVVVIDGLDNSEQSKVLQLLDSVN-LLFTDPDAPFIILMALD 327
BLAST of Kinase D-interacting substrate vs. Ensembl Medaka
Match: ENSORLT00000022722.2 (kinase D interacting substrate 220 [Source:NCBI gene;Acc:101155812]) HSP 1 Score: 174.481 bits (441), Expect = 2.131e-43 Identity = 195/828 (23.55%), Postives = 365/828 (44.08%), Query Frame = 2
Query: 428 YIQALADMLVCPNTNFPISIGLFSRVGAGIRTTHLEKLSDELISLTKINNQKTLQPSLLLFMFPXXXXXXXXXXXXXXTNWIVGVSLGASLSLMSFLLIEIIIFGSNHKR--WGKALRAARWLASFTQSLQLFLDILFRYPHE-NELTTK--PLSIIITHHNLSNYSSASHMTLSIVEKLWKAVEKKYSCLLSRLQISCKNPE-SSTKFKNICCIPIFVLVIIFFSAIIINATLFTIYNKNPMDIFVNKGLLPLFVIMTCIVFLCIFGTLPLVCRTITFLVRSPSRQLKQFMES-ENIDFQASDTLVKNELLKLCQIISIFEAYLNSEKIRMFVFIDAADTESREKLANSIYLVNGLLQMSSNAPIALILAADFQTLNTEWXXXXXXXXXXXXXXXXIFQEISDNFH-LIHSTIHLPIYMEEGIDFNRNSSGKKKY----ASNG-MFCIRGGNSFESLNMVTTGTKQNSVPVSTRKT-SIYXXXXXXXXXXXXXKGASKSIQQLFLTRGDLSDINNKMIKQLTAAAAFIGRLLRINLIEFKLENLISWLSISINWPYQTALMALFLEEQCANLTPKHTLLQIHEQVLSKIGSKDSNPAEIELLARDDRDFGKLDAFIKKDVGATLTFSILKYFVSWSPFMNPYLRYVLRDYLTNKNLAQTRGSRNQLTSPPLTSMALSDPSDVPMDEDLP-------QPIISPSTGSGLVNQITSWSKSSIGNLAHKGDRPKTPIAIKRCMVKLKDGYPGKDLSQLDKYEIGILMCQIEGLSSEENIEKYKEKLIQLNVTGLVLSVCDMDQLQNEMEMNFGDWQLFKTLIYHLRSVEA 2848
Y ALAD+L P PI +GL+++ G+G ++ L+KL DE+ + + Q S L+ + L +V +++G + + +++ SL + ++ ++ FGS +R W A + LA L+L L ++F P E E TT+ P+ + T +N + ++ L A E+++ L +RL N E K+K CCIP FVL + +I L I+ +P ++ VN + + M +V L + + ++ S ++L + + + ++K+E+ + ++ + + ++ RM V ID D ++K+ + V L S P I A+D + + + +++ ++ + N H + + +HLP+++ +R S KK A++G G E+ + Q+ + +R T + + + ++ S + +L +T DI+ + +++L + GRLLR N I F + L SW+ ++ WPY+T+ + LFLEE A ++ +HTL ++E++ I + ++E L D D + F+ LT ++ F+ + ++P LR ++ D R +R Q+ +T L P+ E P QP + S + + SS + P +K K+K P LS L + + Q +G+ + +Y + + N+ G VL C++D+L+ EMEMNFGDWQLF+ ++ R E+
Sbjct: 451 YSSALADILSEPTMQPPICVGLYAQWGSG-KSFLLKKLEDEMKTFAGQQIEPLFQFSWLVIFLTLLLSGMVAVVLGFTVDHKLAIAVSLSLLALLYIFFVLVYFGSRRERESWRWAWLISTRLARQIGYLELLLKLMFVNPLELPEQTTRALPVRFLFTDYNRLSSVGGETSLAEMIATLSDACEREFGFLATRLFRVFMNDEIKGQKWKKTCCIPSFVLFSLTVCCLITGMALLVIFKVDPENMTVNA----VLIAMASVVGLALLLNSRTWWQVAISVLNSQRKRLHSAANNLHKLKSEGFMKVLKHEVEMMSKMAKTIDGFTQNQ-TRMAVIIDGLDACEQDKVLQMLDTVRVLF---SKGPFISIFASDPHII----------IKAINQNLNSVLRDSNINGHDYMRNIVHLPVFLN-----SRGFSTAKKLFMATATSGEALPTEGSLDEENRKLSQNSLAQDQGKLGSRNTLNRRDTYRRRHMQRTITRQMSFDLTKLLVTEDWFCDISPQTMRRLLNIVSITGRLLRANQIIFHWDRLASWIYLTEEWPYRTSWIILFLEETDA-VSDQHTLKNVYERISKNIPTTK----DVEPLLEIDGDIRSFEVFLSSRT-PFLTPRDVEMFLPCTVNLDPKLREIIAD---------VRAAREQMHMGGVTFPTL------PLQEAAPRHQPGYGQPSAACSPTGSFAGSLPAQPHSSYFSGMTGPQHPFYNRHLKSLPFKVKKVGPAVMLSSLSTEAVCERLKQTDGIDPN-MLPQYTATIKKANINGRVLLHCNLDELKKEMEMNFGDWQLFRGMVMEQRYAES 1232
BLAST of Kinase D-interacting substrate vs. Ensembl Medaka
Match: ENSORLT00000018862.2 (kinase D interacting substrate 220 [Source:NCBI gene;Acc:101155812]) HSP 1 Score: 169.088 bits (427), Expect = 1.126e-41 Identity = 196/841 (23.31%), Postives = 367/841 (43.64%), Query Frame = 2
Query: 428 YIQALADMLVCPNTNFPISIGLFSRVGAGIRTTHLEKLSDELISLTKINNQKTLQPSLLLFMFPXXXXXXXXXXXXXXTNWIVGVSLGASLSLMSFLLIEIIIFGSNHKR--WGKALRAARWLASFTQSLQLFLDILFRYPHE-NELTTK--PLSIIITHHNLSNYSSASHMTLSIVEKLWKAVEKKYSCLLSRLQISCKNPE-SSTKFKNICCIPIFVLVIIFFSAIIINATLFTIYNKNPMDIFVNKGLLPLFVIMTCIVFLCIFGTLPLVCRTITFLVRSPSRQLKQFMES-ENIDFQASDTLVKNELLKLCQIISIFEAYLNSEKIRMFVFIDAADTESREKLANSIYLVNGLLQMSSNAPIALILAADFQTLNTEWXXXXXXXXXXXXXXXXIFQEISDNFH-LIHSTIHLPIYMEEGIDFNRNSSGKKKY----ASNG-MFCIRGGNSFESLNMVTTGTKQNSVPVSTRKT-SIYXXXXXXXXXXXXXKGASKSIQQLFLTRGDLSDINNKMIKQLTAAAAFIGRLLRINLIEFKLENLISWLSISINWPYQTALMALFLEEQCANLTPKHTLLQIHEQVLSKIGSKDSNPAEIELLARDDRDFGKLDAFIKKDVGATLTFSILKYFVSWSPFMNPYLRYVLRDYLTNKNLAQTRGSRNQLTSPPLTSMALSDPSDVPMDE---DLPQPIISPSTG--------------SGLVN-QITSWSKSSIGNLAHKGD--RPKTPIAIKRCMVKLKDGYPGKDLSQLDKYEIGILMCQIEGLSSEENIEKYKEKLIQLNVTGLVLSVCDMDQLQNEMEMNFGDWQLFKTLIYHLRSVEA 2848
Y ALAD+L P PI +GL+++ G+G ++ L+KL DE+ + + Q S L+ + L +V +++G + + +++ SL + ++ ++ FGS +R W A + LA L+L L ++F P E E TT+ P+ + T +N + ++ L A E+++ L +RL N E K+K CCIP FVL + +I L I+ +P ++ VN + + M +V L + + ++ S ++L + + + ++K+E+ + ++ + + ++ RM V ID D ++K+ + V L S P I A+D + + + +++ ++ + N H + + +HLP+++ +R S KK A++G G E+ + Q+ + +R T + + + ++ S + +L +T DI+ + +++L + GRLLR N I F + L SW+ ++ WPY+T+ + LFLEE A ++ +HTL ++E++ I + ++E L D D + F+ LT ++ F+ + ++P LR ++ D R +R Q+ +T L P + P SP+ SG+ Q +++ I A +G P PI + P LS L + + Q +G+ + +Y + + N+ G VL C++D+L+ EMEMNFGDWQLF+ ++ R E+
Sbjct: 451 YSSALADILSEPTMQPPICVGLYAQWGSG-KSFLLKKLEDEMKTFAGQQIEPLFQFSWLVIFLTLLLSGMVAVVLGFTVDHKLAIAVSLSLLALLYIFFVLVYFGSRRERESWRWAWLISTRLARQIGYLELLLKLMFVNPLELPEQTTRALPVRFLFTDYNRLSSVGGETSLAEMIATLSDACEREFGFLATRLFRVFMNDEIKGQKWKKTCCIPSFVLFSLTVCCLITGMALLVIFKVDPENMTVNA----VLIAMASVVGLALLLNSRTWWQVAISVLNSQRKRLHSAANNLHKLKSEGFMKVLKHEVEMMSKMAKTIDGFTQNQ-TRMAVIIDGLDACEQDKVLQMLDTVRVLF---SKGPFISIFASDPHII----------IKAINQNLNSVLRDSNINGHDYMRNIVHLPVFLN-----SRGFSTAKKLFMATATSGEALPTEGSLDEENRKLSQNSLAQDQGKLGSRNTLNRRDTYRRRHMQRTITRQMSFDLTKLLVTEDWFCDISPQTMRRLLNIVSITGRLLRANQIIFHWDRLASWIYLTEEWPYRTSWIILFLEETDA-VSDQHTLKNVYERISKNIPTT----KDVEPLLEIDGDIRSFEVFLSSRT-PFLTPRDVEMFLPCTVNLDPKLREIIAD---------VRAAREQMHMGGVTFPTLPLQEAAPRHQPGYGQPSAACSPTGSFAGSLPAQPHSSYFSGMTGPQHPFYNRDVIAEDAREGLPWSPSDPIVVG----------PAVMLSSLSTEAVCERLKQTDGIDPN-MLPQYTATIKKANINGRVLLHCNLDELKKEMEMNFGDWQLFRGMVMEQRYAES 1241
BLAST of Kinase D-interacting substrate vs. Ensembl Medaka
Match: ENSORLT00000018863.2 (kinase D interacting substrate 220 [Source:NCBI gene;Acc:101155812]) HSP 1 Score: 167.548 bits (423), Expect = 3.306e-41 Identity = 196/827 (23.70%), Postives = 366/827 (44.26%), Query Frame = 2
Query: 428 YIQALADMLVCPNTNFPISIGLFSRVGAGIRTTHLEKLSDELISLTKINNQKTLQPSLLLFMFPXXXXXXXXXXXXXXTNWIVGVSLGASLSLMSFLLIEIIIFGSNHKR--WGKALRAARWLASFTQSLQLFLDILFRYPHE-NELTTK--PLSIIITHHNLSNYSSASHMTLSIVEKLWKAVEKKYSCLLSRLQISCKNPE-SSTKFKNICCIPIFVLVIIFFSAIIINATLFTIYNKNPMDIFVNKGLLPLFVIMTCIVFLCIFGTLPLVCRTITFLVRSPSRQLKQFMES-ENIDFQASDTLVKNELLKLCQIISIFEAYLNSEKIRMFVFIDAADTESREKLANSIYLVNGLLQMSSNAPIALILAADFQTLNTEWXXXXXXXXXXXXXXXXIFQEISDNFH-LIHSTIHLPIYMEEGIDFNRNSSGKKKY----ASNG-MFCIRGGNSFESLNMVTTGTKQNSVPVSTRKT-SIYXXXXXXXXXXXXXKGASKSIQQLFLTRGDLSDINNKMIKQLTAAAAFIGRLLRINLIEFKLENLISWLSISINWPYQTALMALFLEEQCANLTPKHTLLQIHEQVLSKIGSKDSNPAEIELLARDDRDFGKLDAFIKKDVGATLTFSILKYFVSWSPFMNPYLRYVLRDYLTNKNLAQTRGSRNQLTSPPLTSMALSDPSDVPMDEDLPQPIISPSTGSGLVNQITSWSKSSIGNLAHKGDRPKTP-IAIKRCMVKLKDGY-----PGKDLSQLDKYEIGILMCQIEGLSSEENIEKYKEKLIQLNVTGLVLSVCDMDQLQNEMEMNFGDWQLFKTLIYHLRSVEA 2848
Y ALAD+L P PI +GL+++ G+G ++ L+KL DE+ + + Q S L+ + L +V +++G + + +++ SL + ++ ++ FGS +R W A + LA L+L L ++F P E E TT+ P+ + T +N + ++ L A E+++ L +RL N E K+K CCIP FVL + +I L I+ +P ++ VN + + M +V L + + ++ S ++L + + + ++K+E+ + ++ + + ++ RM V ID D ++K+ + V L S P I A+D + + + +++ ++ + N H + + +HLP+++ +R S KK A++G G E+ + Q+ + +R T + + + ++ S + +L +T DI+ + +++L + GRLLR N I F + L SW+ ++ WPY+T+ + LFLEE A ++ +HTL ++E++ I + ++E L D D + F+ LT ++ F+ + ++P LR ++ D R +R Q+ +T L P+ E P+ P G S + S G+ A G P P + M + + P LS L + + Q +G+ + +Y + + N+ G VL C++D+L+ EMEMNFGDWQLF+ ++ R E+
Sbjct: 451 YSSALADILSEPTMQPPICVGLYAQWGSG-KSFLLKKLEDEMKTFAGQQIEPLFQFSWLVIFLTLLLSGMVAVVLGFTVDHKLAIAVSLSLLALLYIFFVLVYFGSRRERESWRWAWLISTRLARQIGYLELLLKLMFVNPLELPEQTTRALPVRFLFTDYNRLSSVGGETSLAEMIATLSDACEREFGFLATRLFRVFMNDEIKGQKWKKTCCIPSFVLFSLTVCCLITGMALLVIFKVDPENMTVNA----VLIAMASVVGLALLLNSRTWWQVAISVLNSQRKRLHSAANNLHKLKSEGFMKVLKHEVEMMSKMAKTIDGFTQNQ-TRMAVIIDGLDACEQDKVLQMLDTVRVLF---SKGPFISIFASDPHII----------IKAINQNLNSVLRDSNINGHDYMRNIVHLPVFLN-----SRGFSTAKKLFMATATSGEALPTEGSLDEENRKLSQNSLAQDQGKLGSRNTLNRRDTYRRRHMQRTITRQMSFDLTKLLVTEDWFCDISPQTMRRLLNIVSITGRLLRANQIIFHWDRLASWIYLTEEWPYRTSWIILFLEETDA-VSDQHTLKNVYERISKNIPTT----KDVEPLLEIDGDIRSFEVFLSSRT-PFLTPRDVEMFLPCTVNLDPKLREIIAD---------VRAAREQMHMGGVTFPTL------PLQEAAPRH--QPGYGQ------PSAACSPTGSFA--GSLPAQPHSSYFSGMTGPQHPFYNRVGPAVMLSSLSTEAVCERLKQTDGIDP-NMLPQYTATIKKANINGRVLLHCNLDELKKEMEMNFGDWQLFRGMVMEQRYAES 1221
BLAST of Kinase D-interacting substrate vs. Ensembl Medaka
Match: ENSORLT00000033617.1 (kinase D interacting substrate 220 [Source:NCBI gene;Acc:101155812]) HSP 1 Score: 166.392 bits (420), Expect = 8.224e-41 Identity = 199/853 (23.33%), Postives = 367/853 (43.02%), Query Frame = 2
Query: 428 YIQALADMLVCPNTNFPISIGLFSRVGAGIRTTHLEKLSDELISLTKINNQKTLQPSLLLFMFPXXXXXXXXXXXXXXTNWIVGVSLGASLSLMSFLLIEIIIFGSNHKR--WGKALRAARWLASFTQSLQLFLDILFRYPHE-NELTTK--PLSIIITHHNLSNYSSASHMTLSIVEKLWKAVEKKYSCLLSRLQISCKNPE-SSTKFKNICCIPIFVLVIIFFSAIIINATLFTIYNKNPMDIFVNKGLLPLFVIMTCIVFLCIFGTLPLVCRTI-TFLVRSPSRQLKQFMESEN----IDFQASDTLVKNELLKLCQIISIFEAYLNSEKIRMFVFIDAADTESREKLANSIYLVNGLLQMSSNAPIALILAADFQTLNTEWXXXXXXXXXXXXXXXXIFQEISDNFH-LIHSTIHLPIYMEEGIDFNRNSSGKKKY----ASNG-MFCIRGGNSFESLNMVTTGTKQNSVPVSTRKT-SIYXXXXXXXXXXXXXKGASKSIQQLFLTRGDLSDINNKMIKQLTAAAAFIGRLLRINLIEFKLENLISWLSISINWPYQTALMALFLEEQCANLTPKHTLLQIHEQVLSKIGSKDSNPAEIELLARDDRDFGKLDAFIKKDVGATLTFSILKYFVSWSPFMNPYLRYVLRDYLTNKNLAQTRGSRNQLTSPPLTSMALSDPSDVPMDE---DLPQPIISPS---TGSGLVNQITSWSKSSIG--------------------NLAHKGDRPKTPIAIKRCMVKLKDGY--PGKDLSQLDKYEIGILMCQIEGLSSEENIEKYKEKLIQLNVTGLVLSVCDMDQLQNEMEMNFGDWQLFKTLIYHLRSVEA 2848
Y ALAD+L P PI +GL+++ G+G ++ L+KL DE+ + + Q S L+ + L +V +++G + + +++ SL + ++ ++ FGS +R W A + LA L+L L ++F P E E TT+ P+ + T +N + ++ L A E+++ L +RL N E K+K CCIP FVL + +I L I+ +P ++ VN + + M +V L + L RT + + Q K+ + N + + ++K+E+ + ++ + + ++ RM V ID D ++K+ + V L S P I A+D + + + +++ ++ + N H + + +HLP+++ +R S KK A++G G E+ + Q+ + +R T + + + ++ S + +L +T DI+ + +++L + GRLLR N I F + L SW+ ++ WPY+T+ + LFLEE A ++ +HTL ++E++ I + ++E L D D + F+ LT ++ F+ + ++P LR ++ D R +R Q+ +T L P + P SP+ GS +S+ G + +H P P + G P LS L + + Q +G+ + +Y + + N+ G VL C++D+L+ EMEMNFGDWQLF+ ++ R E+
Sbjct: 451 YSSALADILSEPTMQPPICVGLYAQWGSG-KSFLLKKLEDEMKTFAGQQIEPLFQFSWLVIFLTLLLSGMVAVVLGFTVDHKLAIAVSLSLLALLYIFFVLVYFGSRRERESWRWAWLISTRLARQIGYLELLLKLMFVNPLELPEQTTRALPVRFLFTDYNRLSSVGGETSLAEMIATLSDACEREFGFLATRLFRVFMNDEIKGQKWKKTCCIPSFVLFSLTVCCLITGMALLVIFKVDPENMTVNA----VLIAMASVVGLALL----LNSRTWWQVAISVLNSQRKRLHSAANNLHKLKSEGFMKVLKHEVEMMSKMAKTIDGFTQNQ-TRMAVIIDGLDACEQDKVLQMLDTVRVLF---SKGPFISIFASDPHII----------IKAINQNLNSVLRDSNINGHDYMRNIVHLPVFLN-----SRGFSTAKKLFMATATSGEALPTEGSLDEENRKLSQNSLAQDQGKLGSRNTLNRRDTYRRRHMQRTITRQMSFDLTKLLVTEDWFCDISPQTMRRLLNIVSITGRLLRANQIIFHWDRLASWIYLTEEWPYRTSWIILFLEETDA-VSDQHTLKNVYERISKNIPTT----KDVEPLLEIDGDIRSFEVFLSSRT-PFLTPRDVEMFLPCTVNLDPKLREIIAD---------VRAAREQMHMGGVTFPTLPLQEAAPRHQPGYGQPSAACSPTGSFAGSLPAQPHSSYFSGMTGPQHPFYNRPYFPHHVYHLPRHFSHHAPPPSRPTIKPPGQAQDTSGLVGPAVMLSSLSTEAVCERLKQTDGIDP-NMLPQYTATIKKANINGRVLLHCNLDELKKEMEMNFGDWQLFRGMVMEQRYAES 1259
BLAST of Kinase D-interacting substrate vs. Ensembl Medaka
Match: ENSORLT00000022720.2 (kinase D interacting substrate 220 [Source:NCBI gene;Acc:101155812]) HSP 1 Score: 159.073 bits (401), Expect = 1.143e-38 Identity = 202/873 (23.14%), Postives = 370/873 (42.38%), Query Frame = 2
Query: 428 YIQALADMLVCPNTNFPISIGLFSRVGAGIRTTHLEKLSDELISLTKINNQKTLQPSLLLFMFPXXXXXXXXXXXXXXTNWIVGVSLGASLSLMSFLLIEIIIFGSNHKR--WGKALRAARWLASFTQSLQLFLDILFRYPHE-NELTTK--PLSIIITHHNLSNYSSASHMTLSIVEKLWKAVEKKYSCLLSRLQISCKNPE-SSTKFKNICCIPIFVLVIIFFSAIIINATLFTIYNKNPMDIFVNKGLLPLFVIMTCIVFLCIFGTLPLVCRTI-TFLVRSPSRQLKQFMESEN----IDFQASDTLVKNELLKLCQIISIFEAYLNSEKIRMFVFIDAADTESREKLANSIYLVNGLLQMSSNAPIALILAADFQTLNTEWXXXXXXXXXXXXXXXXIFQEISDNFH-LIHSTIHLPIYMEEGIDFNRNSSGKKKY----ASNG-MFCIRGGNSFESLNMVTTGTKQNSVPVSTRKT-SIYXXXXXXXXXXXXXKGASKSIQQLFLTRGDLSDINNKMIKQLTAAAAFIGRLLRINLIEFKLENLISWLSISINWPYQTALMALFLEEQCANLTPKHTLLQIHEQVLSKIGSKDSNPAEIELLARDDRDFGKLDAFIKKDVGATLTFSILKYFVSWSPFMNPYLRYVLRDYLTNKNLAQTRGSRNQLTSPPLTSMALSDPSDVPMDE---DLPQPIISPS---TGSGLVNQITSWSKSSIG--------------------NLAHKGDRPKTPIA--------IKRCMVKLKDGYPGKDLSQLDKYEIGILMCQIEGLSSEENIEKYKE--------------KLIQLNVTGLVLSVCDMDQLQNEMEMNFGDWQLFKTLIYHLRSVEA 2848
Y ALAD+L P PI +GL+++ G+G ++ L+KL DE+ + + Q S L+ + L +V +++G + + +++ SL + ++ ++ FGS +R W A + LA L+L L ++F P E E TT+ P+ + T +N + ++ L A E+++ L +RL N E K+K CCIP FVL + +I L I+ +P ++ VN + + M +V L + L RT + + Q K+ + N + + ++K+E+ + ++ + + ++ RM V ID D ++K+ + V L S P I A+D + + + +++ ++ + N H + + +HLP+++ +R S KK A++G G E+ + Q+ + +R T + + + ++ S + +L +T DI+ + +++L + GRLLR N I F + L SW+ ++ WPY+T+ + LFLEE A ++ +HTL ++E++ I + ++E L D D + F+ LT ++ F+ + ++P LR ++ D R +R Q+ +T L P + P SP+ GS +S+ G + +H P P V +D G S D +G + + LS+E E+ K+ + + N+ G VL C++D+L+ EMEMNFGDWQLF+ ++ R E+
Sbjct: 451 YSSALADILSEPTMQPPICVGLYAQWGSG-KSFLLKKLEDEMKTFAGQQIEPLFQFSWLVIFLTLLLSGMVAVVLGFTVDHKLAIAVSLSLLALLYIFFVLVYFGSRRERESWRWAWLISTRLARQIGYLELLLKLMFVNPLELPEQTTRALPVRFLFTDYNRLSSVGGETSLAEMIATLSDACEREFGFLATRLFRVFMNDEIKGQKWKKTCCIPSFVLFSLTVCCLITGMALLVIFKVDPENMTVNA----VLIAMASVVGLALL----LNSRTWWQVAISVLNSQRKRLHSAANNLHKLKSEGFMKVLKHEVEMMSKMAKTIDGFTQNQ-TRMAVIIDGLDACEQDKVLQMLDTVRVLF---SKGPFISIFASDPHII----------IKAINQNLNSVLRDSNINGHDYMRNIVHLPVFLN-----SRGFSTAKKLFMATATSGEALPTEGSLDEENRKLSQNSLAQDQGKLGSRNTLNRRDTYRRRHMQRTITRQMSFDLTKLLVTEDWFCDISPQTMRRLLNIVSITGRLLRANQIIFHWDRLASWIYLTEEWPYRTSWIILFLEETDA-VSDQHTLKNVYERISKNIPTT----KDVEPLLEIDGDIRSFEVFLSSRT-PFLTPRDVEMFLPCTVNLDPKLREIIAD---------VRAAREQMHMGGVTFPTLPLQEAAPRHQPGYGQPSAACSPTGSFAGSLPAQPHSSYFSGMTGPQHPFYNRPYFPHHVYHLPRHFSHHAPPPSRPTIKPPGQAQDTSGLDVIAEDAREGLPWSPSDPIVVGPAV-MLSSLSTEAVCERLKQTDGIDPNMLPQYTATIKKANINGRVLLHCNLDELKKEMEMNFGDWQLFRGMVMEQRYAES 1279
BLAST of Kinase D-interacting substrate vs. Planmine SMEST
Match: SMESG000079991.1 (SMESG000079991.1) HSP 1 Score: 2377.82 bits (6161), Expect = 0.000e+0 Identity = 1216/1219 (99.75%), Postives = 1217/1219 (99.84%), Query Frame = 2
Query: 158 MHFSNGKTGMNSIDPSMSGYRSPTGVIISNNEENSKNVGIGAFGPLSSSDATILSGDMNKNNVEDFIGLNYFNYPLDHCGSNIRAQKTVTYIQALADMLVCPNTNFPISIGLFSRVGAGIRTTHLEKLSDELISLTKINNQKTLQPSLLLFMFPXXXXXXXXXXXXXXTNWIVGVSLGASLSLMSFLLIEIIIFGSNHKRWGKALRAARWLASFTQSLQLFLDILFRYPHENELTTKPLSIIITHHNLSNYSSASHMTLSIVEKLWKAVEKKYSCLLSRLQISCKNPESSTKFKNICCIPIFVLVIIFFSAIIINATLFTIYNKNPMDIFVNKGLLPLFVIMTCIVFLCIFGTLPLVCRTITFLVRSPSRQLKQFMESENIDFQASDTLVKNELLKLCQIISIFEAYLNSEKIRMFVFIDAADTESREKLANSIYLVNGLLQMSSNAPIALILAADFQTLNTEWXXXXXXXXXXXXXXXXIFQEISDNFHLIHSTIHLPIYMEEGIDFNRNSSGKKKYASNGMFCIRGGNSFESLNMVTTGTKQNSVPVSTRKTSIYXXXXXXXXXXXXXKGASKSIQQLFLTRGDLSDINNKMIKQLTAAAAFIGRLLRINLIEFKLENLISWLSISINWPYQTALMALFLEEQCANLTPKHTLLQIHEQVLSKIGSKDSNPAEIELLARDDRDFGKLDAFIKKDVGATLTFSILKYFVSWSPFMNPYLRYVLRDYLTNKNLAQTRGSRNQLTSPPLTSMALSDPSDVPMDEDLPQPIISPSTGSGLVNQITSWSKSSIGNLAHKGDRPKTPIAIKRCMVKLKDGYPGKDLSQLDKYEIGILMCQIEGLSSEENIEKYKEKLIQLNVTGLVLSVCDMDQLQNEMEMNFGDWQLFKTLIYHLRSVEAFKSDDLSIVGDPFVDPNSFWXXXXXXXXXXXXXXRISQTCWAGYGRSNKAISKRIRSTMSKSRSQTNNLRSISKRNEVLKVKVNQSNSMKNSSTNTQRKSSLNYSKPGYIYSPLATEDNVISNNYPSESDKSNYPSVPNHHSLSNQNQPNQTSSKYPYSTDNHDPVAFLVKDIKSSSKMKEGSNASLCIHPPGHNDKGEVMKKKSKHSYRSLSQSQERIHLVRARTCSSNNMDRCATYIPVLLQTSLGGCMETGDQECTCDYWFRIEHQNGLCHKIATSEPASRVPSRPQTSPEGSDEEENPRTTAYWPESVTNRPFNGSAV 3814
MHFSNGKTGMNSIDPSMSGYRSPTGVIISNNEENSKNVGIGAFGPLSSSDATILSGDMNKNNVEDFIGLNYFNYPLDHCGSNIRAQKTVTYIQALADMLVCPNTNFPISIGLFSRVGAGIRTTHLEKLSDELISLTKINNQKTLQPSLLLFMFPVFLFIVVGIIVGLGTNWIVGVSLGASLSLMSFLLIEIIIFGSNHKRWGKALRAARWLASFTQSLQLFLDILFRYPHENELTTKPLSIIITHHNLSNYSSASHM LSIVEKLWKAVEKKYSCLLSRLQISCKNPESSTKFKNICCIPIFVLVIIFFSAIIINATLFTIYNKNPMDIFVNKGLLPLFVIMTCIVFLCIFGTLPLVCRTITFLVRSPSRQLKQFMESENIDFQASDTLVKNELLKLCQIISIFEAYLNSEKIRMFVFIDAADTESREKLANSIYLVNGLLQMSSNAPIALILAADFQTLNTEWNSSSNNPNSCSSAKSNIFQEISDNFHLIHSTIHLPIYMEEGIDFNRNSSGKKKYASNGMFCIRGGNSFESLNMVTTGTKQNSVPVSTRKTSIYLSALNSLNADSNSKGASKSIQQLFLTRGDLSDINNKMIKQLTAAAAFIGRLLRINLIEFKLENLISWLSISINWPYQTALMALFLEEQCANLTPKHTLLQIHEQVLSKIGSKDSNPAEIELLARDDRDFGKLDAFIKKDVGATLTFSILKYFVSWSPFMNPYLRYVLRDYLTNKNLAQTRGSRNQLTSPPLTSMALSDPSDVPMDEDLPQPIISPSTGSGLVNQITSWSKSSIGNLAHKGDRPKTPIAIKRCMVKLKDGYPGKDLSQLDKYEIGILMCQIEGLSSEENIEKYKEKLIQLNVTGLVLSVCDMDQLQNEMEMNFGDWQLFKTLIYHLRSVEAFKSDDLSIVGDPFVDPNSFWEEADIDGESDEDDDRISQTCWAGYGRSNKAISKRIRSTMSKSRSQTNNLRSISKRNEVLKVKVNQSNSMKNSSTNTQRKSSLNYSKPGYIYSPLATEDNVISNNYP+ESDKSNYPSVPNH SLSNQNQPNQTSSKYPYSTDNHDPVAFLVKDIKSSSKMKEGSNASLCIHPPGHNDKGEVMKKKSKHSYRSLSQSQERIHLVRARTCSSNNMDRCATYIPVLLQTSLGGCMETGDQECTCDYWFRIEHQNGLCHKIATSEPASRVPSRPQTSPEGSDEEENPRTTAYWPESVTNRPFNGSAV
Sbjct: 1 MHFSNGKTGMNSIDPSMSGYRSPTGVIISNNEENSKNVGIGAFGPLSSSDATILSGDMNKNNVEDFIGLNYFNYPLDHCGSNIRAQKTVTYIQALADMLVCPNTNFPISIGLFSRVGAGIRTTHLEKLSDELISLTKINNQKTLQPSLLLFMFPVFLFIVVGIIVGLGTNWIVGVSLGASLSLMSFLLIEIIIFGSNHKRWGKALRAARWLASFTQSLQLFLDILFRYPHENELTTKPLSIIITHHNLSNYSSASHMNLSIVEKLWKAVEKKYSCLLSRLQISCKNPESSTKFKNICCIPIFVLVIIFFSAIIINATLFTIYNKNPMDIFVNKGLLPLFVIMTCIVFLCIFGTLPLVCRTITFLVRSPSRQLKQFMESENIDFQASDTLVKNELLKLCQIISIFEAYLNSEKIRMFVFIDAADTESREKLANSIYLVNGLLQMSSNAPIALILAADFQTLNTEWNSSSNNPNSCSSAKSNIFQEISDNFHLIHSTIHLPIYMEEGIDFNRNSSGKKKYASNGMFCIRGGNSFESLNMVTTGTKQNSVPVSTRKTSIYLSALNSLNADSNSKGASKSIQQLFLTRGDLSDINNKMIKQLTAAAAFIGRLLRINLIEFKLENLISWLSISINWPYQTALMALFLEEQCANLTPKHTLLQIHEQVLSKIGSKDSNPAEIELLARDDRDFGKLDAFIKKDVGATLTFSILKYFVSWSPFMNPYLRYVLRDYLTNKNLAQTRGSRNQLTSPPLTSMALSDPSDVPMDEDLPQPIISPSTGSGLVNQITSWSKSSIGNLAHKGDRPKTPIAIKRCMVKLKDGYPGKDLSQLDKYEIGILMCQIEGLSSEENIEKYKEKLIQLNVTGLVLSVCDMDQLQNEMEMNFGDWQLFKTLIYHLRSVEAFKSDDLSIVGDPFVDPNSFWEEADIDGESDEDDDRISQTCWAGYGRSNKAISKRIRSTMSKSRSQTNNLRSISKRNEVLKVKVNQSNSMKNSSTNTQRKSSLNYSKPGYIYSPLATEDNVISNNYPNESDKSNYPSVPNHRSLSNQNQPNQTSSKYPYSTDNHDPVAFLVKDIKSSSKMKEGSNASLCIHPPGHNDKGEVMKKKSKHSYRSLSQSQERIHLVRARTCSSNNMDRCATYIPVLLQTSLGGCMETGDQECTCDYWFRIEHQNGLCHKIATSEPASRVPSRPQTSPEGSDEEENPRTTAYWPESVTNRPFNGSAV 1219
BLAST of Kinase D-interacting substrate vs. Planmine SMEST
Match: SMESG000051607.1 (SMESG000051607.1) HSP 1 Score: 624.009 bits (1608), Expect = 0.000e+0 Identity = 350/903 (38.76%), Postives = 541/903 (59.91%), Query Frame = 2
Query: 227 TGVIISNNEENSKNVGIGAFGPLSSSDATILSGDMNKNNVEDFIGLNYFNYPLDHCGSNIRAQKTVTYIQALADMLVCPNTNFPISIGLFSRVGAGIRTTHLEKLSDELISLTKINNQKTLQPSLLLFMFPXXXXXXXXXXXXXXTNWIVGVSLGASLSLMSFLLIEIIIFGSNHKRWGKALRAARWLASFTQSLQLFLDILFRYPHENELTTKPLSIIITHHNLSNYSSASHMTLSIVEKLWKAVEKKYSCLLSRLQISCKNPESSTKFKNICCIPIFVLVIIFFSAIIINATLFTIYNKNPMDIFVNKGLLPLFVIMTCIVFLCIFGTLPLVCRTITFLVRSPSRQLKQFMESENIDFQASDTLVKNELLKLCQIISIFEAYLNSEKIRMFVFIDAADTESREKLANSIYLVNGLLQMSSNAPIALILAADFQTLNTEWXXXXXXXXXXXXXXXXIFQEISDNFHLIHSTIHLPIYMEEGIDFNRNSSGKKKYA-SNGMFCIRGGNSFESLNMVTTGTKQNSVPVSTRKTSIYXXXXXXXXXXXXXKGASKSIQQLFLTRGDLSDINNKMIKQLTAAAAFIGRLLRINLIEFKLENLISWLSISINWPYQTALMALFLEEQCANLTPKHTLLQIHEQVLSKIGSKDSNPAEIELLARDDRDFGKLDAFIKKDVGATLTFSILKYFVSWSPFMNPYLRYVLRDYLTNKNLAQTRGSRNQLTSPPLTSMALSDPSDVPMDEDLPQPIISPSTGSGLVNQITSWSKSSIGNLAHK--GDRPKTPIAIKRCMVKLKDGYPGKDLSQLDKYEIGILMCQIEGLSSEENIEKYKEKLIQLNVTGLVLSVCDMDQLQNEMEMNFGDWQLFKTLIYHLRSVEAFK------SDDLSIVGDPFVDPNSFW 2908
+G+II N + N A PL+S D + S + +K N ++ LN F + H + ++ + I+ LAD++ PN P+ +G+F+R GIRTTHL KL++E+ N+ LQPS LLF+FP I+ G ++G+ NW+ G+ LG + ++ F LI +I+FGS +R KAL A++ LA F + + L L ILF YP + +L KP+ II HNL++Y SA H+TL+IVE +W AVEKKY +L+RLQ SCK+ +S ++FK +CC+P FVLVI+FF A++IN+TL +IY+K + +F + LLP+FV+MTCI+FL FG LP+ C+ + L +SP+R + + EN D+ D LVK EL ++C +IS FEAY E++R+F+ +++ D +R+KLAN +YL+ L M + P ILAADF TL +W + + + +FQ+ SD+FHL+HST HLP+YM+E F K ++ +N MFC+RGG+SFESLNM +G+K +P+ S + + K IQQLFL++ DL +IN K+IKQL +A F+ ++L+IN IEF + LI W+ + INWP++ L+ F+ ++ +++PK +L ++H+ +L S+ N + LLA+DD+ F++ + + L+ LK F S S F+NPY++ ++ ++++ K + S P + D+P +I + V+QI S +H G+ KTPI +++C K+KD KDLSQL K E+ L+ I+ + S + + +Y+ +LI LNVTGLVLS+CD+D LQ E+EMNFGD+Q+FK LI +LR +E+ K S+D+ + D +FW
Sbjct: 11 SGLIIPNEDCIKMNYNEQA-SPLTSPD--MFSSNKDKKN---WLNLNSFQF---HNQTQSARERVINIIKVLADLMTEPNVPHPLYVGIFNRTTPGIRTTHLNKLAEEISHTCAKQNEILLQPSRLLFLFPCASSIISGFVLGITINWVFGIVLGVASMVLIFCLIGMILFGSKGRRSSKALFASKKLAKFMERVDLLLQILFCYPSDTDLINKPIVPIILTHNLADYPSAHHLTLAIVESIWIAVEKKYLKILTRLQCSCKDTDSESRFKQVCCVPTFVLVIVFFLALMINSTLLSIYHKREITMFTDTTLLPIFVLMTCIIFLGFFGCLPVFCKVLIMLFKSPARAISEMKNIENPDYSLVDNLVKLELQRVCMLISTFEAYQAKEQLRIFIMVNSTDNCNRQKLANCVYLLQNLTTMRTTVPTVSILAADFYTLINDWTLLTPISSEIN-----LFQQFSDSFHLLHSTFHLPVYMDEIFSFIPTLPQKTSFSNTNEMFCLRGGSSFESLNMAISGSKSTPLPI----VSANKQNCSIKMESIEMDYSLKKIQQLFLSKPDLLEINTKVIKQLLISAIFLEKILKINKIEFVWDRLIDWIILCINWPFRINLIINFVIDEFPSISPKASLKKLHDDILECFNSEHLNNKDFLLLAQDDKSISIFGNFLESESFSNLSIVDLKIFSSHSSFLNPYIQSIITEHMSQK-------------------LYSSRPKSL---GDIPTTVIQTN-----VSQINS---------SHNINGETLKTPIFVRKCFTKVKDNMRIKDLSQLSKKEVINLLNHIDTMDSTK-LGRYQTRLIDLNVTGLVLSICDLDNLQRELEMNFGDFQIFKALILYLRELESLKMISLTDSEDIGELSPQIFDNLAFW 858
BLAST of Kinase D-interacting substrate vs. Planmine SMEST
Match: SMESG000051607.1 (SMESG000051607.1) HSP 1 Score: 621.313 bits (1601), Expect = 0.000e+0 Identity = 355/904 (39.27%), Postives = 546/904 (60.40%), Query Frame = 2
Query: 227 TGVIISNNEENSKNVGIGAFGPLSSSDATILSGDMNKNNVEDFIGLNYFNYPLDHCGSNIRAQKTVTYIQALADMLVCPNTNFPISIGLFSRVGAGIRTTHLEKLSDELISLTKINNQKTLQPSLLLFMFPXXXXXXXXXXXXXXTNWIVGVSLGASLSLMSFLLIEIIIFGSNHKRWGKALRAARWLASFTQSLQLFLDILFRYPHENELTTKPLSIIITHHNLSNYSSASHMTLSIVEKLWKAVEKKYSCLLSRLQISCKNPESSTKFKNICCIPIFVLVIIFFSAIIINATLFTIYNKNPMDIFVNKGLLPLFVIMTCIVFLCIFGTLPLVCRTITFLVRSPSRQLKQFMESENIDFQASDTLVKNELLKLCQIISIFEAYLNSEKIRMFVFIDAADTESREKLANSIYLVNGLLQMSSNAPIALILAADFQTLNTEWXXXXXXXXXXXXXXXXIFQEISDNFHLIHSTIHLPIYMEEGIDFNRNSSGKKKYA-SNGMFCIRGGNSFESLNMVTTGTKQNSVPVSTRKTSIYXXXXXXXXXXXXXKGASKSIQQLFLTRGDLSDINNKMIKQLTAAAAFIGRLLRINLIEFKLENLISWLSISINWPYQTALMALFLEEQCANLTPKHTLLQIHEQVLSKIGSKDSNPAEIELLARDDRDFGKLDAFIKKDVGATLTFSILKYFVSWSPFMNPYLRYVLRD-YLTNKNLAQTRGSRNQLTSPPLTSMALSDPSDVPMDEDLPQPIISPSTGSGLVNQITSWSKSSIGNLAH--KGDRPKTPIAIKRCMVKLKDGYPGKDLSQLDKYEIGILMCQIEGLSSEENIEKYKEKLIQLNVTGLVLSVCDMDQLQNEMEMNFGDWQLFKTLIYHLRSVEAFK------SDDLSIVGDPFVDPNSFW 2908
+G+II N + N A PL+S D + S + +K N ++ LN F + H + ++ + I+ LAD++ PN P+ +G+F+R GIRTTHL KL++E+ N+ LQPS LLF+FP I+ G ++G+ NW+ G+ LG + ++ F LI +I+FGS +R KAL A++ LA F + + L L ILF YP + +L KP+ II HNL++Y SA H+TL+IVE +W AVEKKY +L+RLQ SCK+ +S ++FK +CC+P FVLVI+FF A++IN+TL +IY+K + +F + LLP+FV+MTCI+FL FG LP+ C+ + L +SP+R + + EN D+ D LVK EL ++C +IS FEAY E++R+F+ +++ D +R+KLAN +YL+ L M + P ILAADF TL +W + S++ N+FQ+ SD+FHL+HST HLP+YM+E F K ++ +N MFC+RGG+SFESLNM +G+K +P+ S + + K IQQLFL++ DL +IN K+IKQL +A F+ ++L+IN IEF + LI W+ + INWP++ L+ F+ ++ +++PK +L ++H+ +L S+ N + LLA+DD+ F++ + + L+ LK F S S F+NPY++ ++ + YL ++++Q S S P + D+P +I + V+QI S +H G+ KTPI +++C K+KD KDLSQL K E+ L+ I+ + S + + +Y+ +LI LNVTGLVLS+CD+D LQ E+EMNFGD+Q+FK LI +LR +E+ K S+D+ + D +FW
Sbjct: 11 SGLIIPNEDCIKMNYNEQA-SPLTSPD--MFSSNKDKKN---WLNLNSFQF---HNQTQSARERVINIIKVLADLMTEPNVPHPLYVGIFNRTTPGIRTTHLNKLAEEISHTCAKQNEILLQPSRLLFLFPCASSIISGFVLGITINWVFGIVLGVASMVLIFCLIGMILFGSKGRRSSKALFASKKLAKFMERVDLLLQILFCYPSDTDLINKPIVPIILTHNLADYPSAHHLTLAIVESIWIAVEKKYLKILTRLQCSCKDTDSESRFKQVCCVPTFVLVIVFFLALMINSTLLSIYHKREITMFTDTTLLPIFVLMTCIIFLGFFGCLPVFCKVLIMLFKSPARAISEMKNIENPDYSLVDNLVKLELQRVCMLISTFEAYQAKEQLRIFIMVNSTDNCNRQKLANCVYLLQNLTTMRTTVPTVSILAADFYTLINDW-----TLLTPISSEINLFQQFSDSFHLLHSTFHLPVYMDEIFSFIPTLPQKTSFSNTNEMFCLRGGSSFESLNMAISGSKSTPLPI----VSANKQNCSIKMESIEMDYSLKKIQQLFLSKPDLLEINTKVIKQLLISAIFLEKILKINKIEFVWDRLIDWIILCINWPFRINLIINFVIDEFPSISPKASLKKLHDDILECFNSEHLNNKDFLLLAQDDKSISIFGNFLESESFSNLSIVDLKIFSSHSSFLNPYIQSIITEIYL--EHMSQKLYS--------------SRPKSL---GDIPTTVIQTN-----VSQINS---------SHNINGETLKTPIFVRKCFTKVKDNMRIKDLSQLSKKEVINLLNHIDTMDSTK-LGRYQTRLIDLNVTGLVLSICDLDNLQRELEMNFGDFQIFKALILYLRELESLKMISLTDSEDIGELSPQIFDNLAFW 862
BLAST of Kinase D-interacting substrate vs. Planmine SMEST
Match: SMESG000080369.1 (SMESG000080369.1) HSP 1 Score: 125.176 bits (313), Expect = 1.695e-28 Identity = 103/373 (27.61%), Postives = 181/373 (48.53%), Query Frame = 2
Query: 434 QALADMLVCPNTNFPISIGLFSRVGAGIRTTHLEKLSD----ELISLTKINNQKTLQPSLLLFMFPXXXXXXXXXXXXXXTNWIVGVSLGASLSLMSFLLIEIIIFGSNHKRWGKALRAARWLASFTQSLQLFLDILFRYPHENE-LTTKPLSIIITHHNLSNYSSASHMTLSIVEKLWKAVEKKYSCLLSRLQISCKNPE-SSTKFKNICCIPIFVLVIIFFSAIIINATLFTIYNKNPMDIFVNKGLLPLFVIMTCIVFLCIFGTLPLVCRTITFLVRSPSRQLKQFMESENIDFQAS-DTLVKNELLKLCQIISIFEAYLNSEKIRMFVFIDAADTESREKLANSIYLVNGLLQMSSNAPIALILAADFQ 1531
+ L D ++ TN+ IG+F + +R ++ +L D + S T INN K L F I + + + W VG+++G ++ + F+ I +I+ G+ + W AL+A L + +S +L+L + F P + + + L I TH LSNYS+ H +E +W ++EK Y+ RL + N + KFK ICC+P FV I ++ T+ + N +F NK +L F + ++F+CI L L + I ++ SP+ LK F++S NI +AS T + +E + Q++S + Y N + + F+DA T + L + LL+ +++ + ++ D Q
Sbjct: 105 ETLIDSVLLCQTNYSFIIGIFFKYAKFLRGQNILRLKDFIEKKYFSST-INNIKFRLTPFLSFTICFLSVICGYLCGLIIS-WEVGLAVGFTILFVLFIGIILILIGTYCQEWRMALQAGIVLCNAIRSFKLWLRLFFLNPSQGQNYNSNELRFIYTHLILSNYSNNFHAIAKFIESIWSSIEKNYNNFFIRLMKASLNQDYGERKFKRICCLPTFVYFIGTLICVLGIGTILKVNETNLSTVFNNKLILIGFSFLAIVLFICIIILLFLTVKIIFCVIHSPTHNLKGFLKSYNIYGEASTQTFLMSEFNSVLQLLSQLDKYENDHRSFIVTFVDATGTTDLKVLMEFCSFCHVLLKNNNDITMCFLILIDNQ 475 HSP 2 Score: 100.908 bits (250), Expect = 5.270e-21 Identity = 79/322 (24.53%), Postives = 151/322 (46.89%), Query Frame = 2
Query: 1880 KSIQQLFLTRGDLSDINNKMIKQLTAAAAFIGRLLRINLIEFKLENLISWLSISINWPYQTALMALFLEEQCANLTPKHTLLQIHEQVLSKIGSKDSNPAEIELLARDDRDFGKLDAFIKKDVGATLTFSILKYFVSWSPFMNPYLRYVLRDYLTNKNLAQTRGSRNQLTSPPLTSMALSDPSDVPMDEDLPQPIISPSTGSGLVNQITSWSKSSIGNLAHKGDRPKTPIAIKRCMVKLKDGYPGKDLSQLDKYEIGILMCQIEGLSSEENIEKYKEKLIQLNVTGLVLSVCDMDQLQNEMEMNFGDWQLFKTLIYHLRSVE 2845
++I + + LSD N +K+L + F +L ++ I + LI ++ +WP+ + M LF+ L +L +++E ++ + + L++ D D L++ + + LT N ++ L++ + + +T+ + N L SD S + P I P + N+I L KTP +++C+ +K+ + LS++ + ++ QI+ +S +ENI KY+EK+ ++N GLVL+ CD ++L+ E++M+FGDW LF TLI +L+S+E
Sbjct: 578 QNISDYLIIKSKLSDFNVTYVKELVITSMFSYKLSQLQNINTDIYKLIDLVTFYYHWPFLYSWMTLFIVNNIYVLKKSLSLRKLYENIMRNMLQSMIEEQFLLELSKLDLDLNVLESMMMHNYIGELTIEEFLILTHACHLQNIPMQNFLKNAVKKNEMKKTKCT-NSLYD------GFSDRSYSSKTNNCP---IYPPNNT---NEI----------LLDSEKHLKTPKNVQKCIQNIKELFSSLILSEISLDNVIWMISQIDEIS-KENIAKYQEKIKEINANGLVLATCDPNELRKELQMSFGDWHLFYTLISYLKSIE 875
BLAST of Kinase D-interacting substrate vs. Planmine SMEST
Match: SMESG000080369.1 (SMESG000080369.1) HSP 1 Score: 125.176 bits (313), Expect = 2.015e-28 Identity = 103/373 (27.61%), Postives = 181/373 (48.53%), Query Frame = 2
Query: 434 QALADMLVCPNTNFPISIGLFSRVGAGIRTTHLEKLSD----ELISLTKINNQKTLQPSLLLFMFPXXXXXXXXXXXXXXTNWIVGVSLGASLSLMSFLLIEIIIFGSNHKRWGKALRAARWLASFTQSLQLFLDILFRYPHENE-LTTKPLSIIITHHNLSNYSSASHMTLSIVEKLWKAVEKKYSCLLSRLQISCKNPE-SSTKFKNICCIPIFVLVIIFFSAIIINATLFTIYNKNPMDIFVNKGLLPLFVIMTCIVFLCIFGTLPLVCRTITFLVRSPSRQLKQFMESENIDFQAS-DTLVKNELLKLCQIISIFEAYLNSEKIRMFVFIDAADTESREKLANSIYLVNGLLQMSSNAPIALILAADFQ 1531
+ L D ++ TN+ IG+F + +R ++ +L D + S T INN K L F I + + + W VG+++G ++ + F+ I +I+ G+ + W AL+A L + +S +L+L + F P + + + L I TH LSNYS+ H +E +W ++EK Y+ RL + N + KFK ICC+P FV I ++ T+ + N +F NK +L F + ++F+CI L L + I ++ SP+ LK F++S NI +AS T + +E + Q++S + Y N + + F+DA T + L + LL+ +++ + ++ D Q
Sbjct: 111 ETLIDSVLLCQTNYSFIIGIFFKYAKFLRGQNILRLKDFIEKKYFSST-INNIKFRLTPFLSFTICFLSVICGYLCGLIIS-WEVGLAVGFTILFVLFIGIILILIGTYCQEWRMALQAGIVLCNAIRSFKLWLRLFFLNPSQGQNYNSNELRFIYTHLILSNYSNNFHAIAKFIESIWSSIEKNYNNFFIRLMKASLNQDYGERKFKRICCLPTFVYFIGTLICVLGIGTILKVNETNLSTVFNNKLILIGFSFLAIVLFICIIILLFLTVKIIFCVIHSPTHNLKGFLKSYNIYGEASTQTFLMSEFNSVLQLLSQLDKYENDHRSFIVTFVDATGTTDLKVLMEFCSFCHVLLKNNNDITMCFLILIDNQ 481 HSP 2 Score: 100.908 bits (250), Expect = 5.605e-21 Identity = 79/322 (24.53%), Postives = 151/322 (46.89%), Query Frame = 2
Query: 1880 KSIQQLFLTRGDLSDINNKMIKQLTAAAAFIGRLLRINLIEFKLENLISWLSISINWPYQTALMALFLEEQCANLTPKHTLLQIHEQVLSKIGSKDSNPAEIELLARDDRDFGKLDAFIKKDVGATLTFSILKYFVSWSPFMNPYLRYVLRDYLTNKNLAQTRGSRNQLTSPPLTSMALSDPSDVPMDEDLPQPIISPSTGSGLVNQITSWSKSSIGNLAHKGDRPKTPIAIKRCMVKLKDGYPGKDLSQLDKYEIGILMCQIEGLSSEENIEKYKEKLIQLNVTGLVLSVCDMDQLQNEMEMNFGDWQLFKTLIYHLRSVE 2845
++I + + LSD N +K+L + F +L ++ I + LI ++ +WP+ + M LF+ L +L +++E ++ + + L++ D D L++ + + LT N ++ L++ + + +T+ + N L SD S + P I P + N+I L KTP +++C+ +K+ + LS++ + ++ QI+ +S +ENI KY+EK+ ++N GLVL+ CD ++L+ E++M+FGDW LF TLI +L+S+E
Sbjct: 584 QNISDYLIIKSKLSDFNVTYVKELVITSMFSYKLSQLQNINTDIYKLIDLVTFYYHWPFLYSWMTLFIVNNIYVLKKSLSLRKLYENIMRNMLQSMIEEQFLLELSKLDLDLNVLESMMMHNYIGELTIEEFLILTHACHLQNIPMQNFLKNAVKKNEMKKTKCT-NSLYD------GFSDRSYSSKTNNCP---IYPPNNT---NEI----------LLDSEKHLKTPKNVQKCIQNIKELFSSLILSEISLDNVIWMISQIDEIS-KENIAKYQEKIKEINANGLVLATCDPNELRKELQMSFGDWHLFYTLISYLKSIE 881 The following BLAST results are available for this feature:
BLAST of Kinase D-interacting substrate vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Kinase D-interacting substrate vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Kinase D-interacting substrate vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Kinase D-interacting substrate vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 3
BLAST of Kinase D-interacting substrate vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Kinase D-interacting substrate vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 4
BLAST of Kinase D-interacting substrate vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 3
BLAST of Kinase D-interacting substrate vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Kinase D-interacting substrate vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Kinase D-interacting substrate vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of Kinase D-interacting substrate vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Kinase D-interacting substrate vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 1
BLAST of Kinase D-interacting substrate vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Kinase D-interacting substrate vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30020234 ID=SMED30020234|Name=Kinase D-interacting substrate|organism=Schmidtea mediterranea sexual|type=transcript|length=4257bpback to top protein sequence of SMED30020234-orf-1 >SMED30020234-orf-1 ID=SMED30020234-orf-1|Name=SMED30020234-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1220bp MHFSNGKTGMNSIDPSMSGYRSPTGVIISNNEENSKNVGIGAFGPLSSSDback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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