DUF3421 domain-containing protein

Overview
NameDUF3421 domain-containing protein
Smed IDSMED30020231
Length (bp)573
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of DUF3421 domain-containing protein (SMED30020231) t-SNE clustered cells

Violin plots show distribution of expression levels for DUF3421 domain-containing protein (SMED30020231) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of DUF3421 domain-containing protein (SMED30020231) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for DUF3421 domain-containing protein (SMED30020231) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30020231

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 9

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
gutSMED30020231SMESG000046728.1 SmedASXL_004676SmedAsxl_ww_GCZZ01PMID:29158443
He et al., 2017		whole organism asexual adult colorimetric in situ hybridization evidence
pigment cellSMED30020231SMESG000046728.1 SmedASXL_004676SmedAsxl_ww_GCZZ01PMID:29158443
He et al., 2017		whole organism asexual adult RNA-sequencing evidence
gutSMED30020231SMESG000046728.1 dd_Smed_v4_1984_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30020231SMESG000046728.1 dd_Smed_v4_1984_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30020231SMESG000046728.1 dd_Smed_v4_1984_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30020231SMESG000046728.1 Contig1054newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30020231SMESG000046728.1 Contig1054uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30020231SMESG000046728.1 Contig50153newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30020231SMESG000046728.1 Contig50153uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of DUF3421 domain-containing protein vs. Ensembl Fly
Match: CG13321 (gene:FBgn0033787 transcript:FBtr0345371)

HSP 1 Score: 91.6633 bits (226), Expect = 2.461e-22
Identity = 53/139 (38.13%), Postives = 71/139 (51.08%), Query Frame = 2 
Query:  140 YGQIPYNAISAG-----DGVFIARAKTGSDVTPGMVRIGYNNAFFPFDGREEFITNYEVLCDTGLHVFGPEIVWKHMGGGCVPSGAIVGGNTSNGEPLYIARAHVNGELCVGKVCQSHKCAYMPNQSKEHRIQNYEILC 541
            YG +P  AI AG     D +F+ RA    ++ P  V  G   A+ P+ G+E    ++EVL        G    W    GG VP  AI  G T  GEPLY+ R +  G L  GKV  SH+C Y+P   +EHR++ YE+L 
Sbjct:   12 YGSLPPGAILAGHDSDQDPIFVGRAYHNGEMLPAKVVPGKQQAYVPWGGQEISKHDFEVLV-------GDHFSWIPSSGGSVPPHAIQVGQTGEGEPLYVGRGYFQGSLTPGKVHPSHQCLYIPYGGQEHRLEAYEVLV 143

HSP 2 Score: 87.4261 bits (215), Expect = 8.233e-21
Identity = 49/125 (39.20%), Postives = 69/125 (55.20%), Query Frame = 2 
Query:  167 SAGDGVFIARAKTGSDVTPGMVRIGYNNAFFPFDGREEFITNYEVLCDTGLHVFGPEIVWKHMGGGCVPSGAIVGGNTSNGEPLYIARAHVNGELCVGKVCQSHKCAYMPNQSKEHRIQNYEILC 541
            + GD +++ RA    D+ P  V      A+ P+ G E    +YE+L   G   +G    W H   G VP  A++ G TS+GEPL+I RAH +G L  GK+ QSH C Y+P   +E RI +YE+L 
Sbjct:  167 ADGDQIYVGRAYHEGDLLPAKVIPNKGCAYVPYGGGEVVKHDYELLAGYG---YG----WVHDSHGNVPGNAVLCGRTSDGEPLFIGRAHHHGSLTPGKIHQSHHCLYIPFDGEEVRIDHYEVLV 284

HSP 3 Score: 75.0998 bits (183), Expect = 3.253e-16
Identity = 46/145 (31.72%), Postives = 72/145 (49.66%), Query Frame = 2 
Query:  122 SWLPGQYGQIPYNAISAG-----DGVFIARAKTGSDVTPGMVRIGYNNAFFPFDGREEFITNYEVLCDTGLHVFGPEIVWKHMGGGCVPSGAIVGGNTSNGEPLYIARAHVNGELCVGKVCQSHKCAYMPNQSKEHRIQNYEILC 541
            SW+P   G +P +AI  G     + +++ R      +TPG V   +   + P+ G+E  +  YEVL         PE      G G VP G +VGG+ ++G+ +Y+ RA+  G+L   KV  +  CAY+P    E    +YE+L 
Sbjct:   77 SWIPSSGGSVPPHAIQVGQTGEGEPLYVGRGYFQGSLTPGKVHPSHQCLYIPYGGQEHRLEAYEVLVQ-------PETWIASSGRGIVP-GTVVGGHDADGDQIYVGRAYHEGDLLPAKVIPNKGCAYVPYGGGEVVKHDYELLA 213

HSP 4 Score: 46.9802 bits (110), Expect = 2.971e-6
Identity = 24/66 (36.36%), Postives = 36/66 (54.55%), Query Frame = 2 
Query:  125 WLPGQYGQIPYNAI-----SAGDGVFIARAKTGSDVTPGMVRIGYNNAFFPFDGREEFITNYEVLC 307
            W+   +G +P NA+     S G+ +FI RA     +TPG +   ++  + PFDG E  I +YEVL 
Sbjct:  219 WVHDSHGNVPGNAVLCGRTSDGEPLFIGRAHHHGSLTPGKIHQSHHCLYIPFDGEEVRIDHYEVLV 284
[Close]
BLAST of DUF3421 domain-containing protein vs. Ensembl Fly
Match: CG13321 (gene:FBgn0033787 transcript:FBtr0087792)

HSP 1 Score: 91.6633 bits (226), Expect = 2.461e-22
Identity = 53/139 (38.13%), Postives = 71/139 (51.08%), Query Frame = 2 
Query:  140 YGQIPYNAISAG-----DGVFIARAKTGSDVTPGMVRIGYNNAFFPFDGREEFITNYEVLCDTGLHVFGPEIVWKHMGGGCVPSGAIVGGNTSNGEPLYIARAHVNGELCVGKVCQSHKCAYMPNQSKEHRIQNYEILC 541
            YG +P  AI AG     D +F+ RA    ++ P  V  G   A+ P+ G+E    ++EVL        G    W    GG VP  AI  G T  GEPLY+ R +  G L  GKV  SH+C Y+P   +EHR++ YE+L 
Sbjct:   12 YGSLPPGAILAGHDSDQDPIFVGRAYHNGEMLPAKVVPGKQQAYVPWGGQEISKHDFEVLV-------GDHFSWIPSSGGSVPPHAIQVGQTGEGEPLYVGRGYFQGSLTPGKVHPSHQCLYIPYGGQEHRLEAYEVLV 143

HSP 2 Score: 87.4261 bits (215), Expect = 8.233e-21
Identity = 49/125 (39.20%), Postives = 69/125 (55.20%), Query Frame = 2 
Query:  167 SAGDGVFIARAKTGSDVTPGMVRIGYNNAFFPFDGREEFITNYEVLCDTGLHVFGPEIVWKHMGGGCVPSGAIVGGNTSNGEPLYIARAHVNGELCVGKVCQSHKCAYMPNQSKEHRIQNYEILC 541
            + GD +++ RA    D+ P  V      A+ P+ G E    +YE+L   G   +G    W H   G VP  A++ G TS+GEPL+I RAH +G L  GK+ QSH C Y+P   +E RI +YE+L 
Sbjct:  167 ADGDQIYVGRAYHEGDLLPAKVIPNKGCAYVPYGGGEVVKHDYELLAGYG---YG----WVHDSHGNVPGNAVLCGRTSDGEPLFIGRAHHHGSLTPGKIHQSHHCLYIPFDGEEVRIDHYEVLV 284

HSP 3 Score: 75.0998 bits (183), Expect = 3.253e-16
Identity = 46/145 (31.72%), Postives = 72/145 (49.66%), Query Frame = 2 
Query:  122 SWLPGQYGQIPYNAISAG-----DGVFIARAKTGSDVTPGMVRIGYNNAFFPFDGREEFITNYEVLCDTGLHVFGPEIVWKHMGGGCVPSGAIVGGNTSNGEPLYIARAHVNGELCVGKVCQSHKCAYMPNQSKEHRIQNYEILC 541
            SW+P   G +P +AI  G     + +++ R      +TPG V   +   + P+ G+E  +  YEVL         PE      G G VP G +VGG+ ++G+ +Y+ RA+  G+L   KV  +  CAY+P    E    +YE+L 
Sbjct:   77 SWIPSSGGSVPPHAIQVGQTGEGEPLYVGRGYFQGSLTPGKVHPSHQCLYIPYGGQEHRLEAYEVLVQ-------PETWIASSGRGIVP-GTVVGGHDADGDQIYVGRAYHEGDLLPAKVIPNKGCAYVPYGGGEVVKHDYELLA 213

HSP 4 Score: 46.9802 bits (110), Expect = 2.971e-6
Identity = 24/66 (36.36%), Postives = 36/66 (54.55%), Query Frame = 2 
Query:  125 WLPGQYGQIPYNAI-----SAGDGVFIARAKTGSDVTPGMVRIGYNNAFFPFDGREEFITNYEVLC 307
            W+   +G +P NA+     S G+ +FI RA     +TPG +   ++  + PFDG E  I +YEVL 
Sbjct:  219 WVHDSHGNVPGNAVLCGRTSDGEPLFIGRAHHHGSLTPGKIHQSHHCLYIPFDGEEVRIDHYEVLV 284
[Close]
BLAST of DUF3421 domain-containing protein vs. Ensembl Fly
Match: CG10527 (gene:FBgn0034583 transcript:FBtr0071541)

HSP 1 Score: 83.9593 bits (206), Expect = 2.330e-19
Identity = 48/141 (34.04%), Postives = 72/141 (51.06%), Query Frame = 2 
Query:  125 WLPGQYGQIPYNAISAG----DGVFIARAKTGSDVTPGMVRIGYNNAFFPFDGREEFITNYEVLCDTGLHVFGPEIVWKHMGGGCVPSGAIVGGNTSNGEPLYIARAHVNGELCVGKVCQSHKCAYMPNQSKEHRIQNYEI 535
            W+P   G++P NA+  G    + ++IARA+   D+ PG +   +   +  + G E     YEVLC  G         W  +  G +P  A+  G T+ GEPL+I RA  +G + VGKV  SH C Y+P   +E   + +EI
Sbjct:  159 WVPAANGEVPPNALEGGFDSSEQLYIARARHEGDLIPGKLHPSHGVTYVAWGGGEHGHAEYEVLCAGGGQ-------WLPVDAGNIPPNALPAGETAEGEPLFIGRATHDGTITVGKVQPSHGCCYIPYGGEELAYKEFEI 292

HSP 2 Score: 46.595 bits (109), Expect = 4.523e-6
Identity = 28/66 (42.42%), Postives = 34/66 (51.52%), Query Frame = 2 
Query:  344 WKHMGGGCVPSGAIVGGNTSNGEPLYIARAHVNGELCVGKVCQSHKCAYMPNQSKEHRIQNYEILC 541
            W     G VP  A+ GG  S+ E LYIARA   G+L  GK+  SH   Y+     EH    YE+LC
Sbjct:  159 WVPAANGEVPPNALEGGFDSS-EQLYIARARHEGDLIPGKLHPSHGVTYVAWGGGEHGHAEYEVLC 223
[Close]
BLAST of DUF3421 domain-containing protein vs. Ensembl Fly
Match: CG31086 (gene:FBgn0051086 transcript:FBtr0084989)

HSP 1 Score: 78.5666 bits (192), Expect = 1.340e-18
Identity = 53/145 (36.55%), Postives = 69/145 (47.59%), Query Frame = 2 
Query:  122 SWLPGQYGQIPYNAI-----SAGDGVFIARAKTGSDVTPGMVRIGYNNAFFPFDGREEFITNYEVLCDTGLHVFGPEIVWKHMGGGCVPSGAIVGGNTSNGEPLYIARAHVNGELCVGKVCQSHKCAYMPNQSKEHRIQNYEILC 541
            SWL    G IP  A+     S GD +FI RA   +D+ P  +      A+  +  +E  + NYEVL        G    W     G VP GA+  G   +GE LY  R +  G L VGKV  SH C Y+P  S+E +I  YE+L 
Sbjct:    5 SWLHFSNGAIPQAAVVAGHDSDGDTIFIGRAFYCNDMLPAKIIPNKGKAYVAYANQEVELENYEVLS-------GFNYEWLPAENGEVPPGAVKVGQNVDGETLYAGRGYHAGSLTVGKVHPSHGCLYIPYDSEEVKIFAYEVLS 142
[Close]
BLAST of DUF3421 domain-containing protein vs. Ensembl Fly
Match: CG31086 (gene:FBgn0051086 transcript:FBtr0307341)

HSP 1 Score: 78.5666 bits (192), Expect = 1.340e-18
Identity = 53/145 (36.55%), Postives = 69/145 (47.59%), Query Frame = 2 
Query:  122 SWLPGQYGQIPYNAI-----SAGDGVFIARAKTGSDVTPGMVRIGYNNAFFPFDGREEFITNYEVLCDTGLHVFGPEIVWKHMGGGCVPSGAIVGGNTSNGEPLYIARAHVNGELCVGKVCQSHKCAYMPNQSKEHRIQNYEILC 541
            SWL    G IP  A+     S GD +FI RA   +D+ P  +      A+  +  +E  + NYEVL        G    W     G VP GA+  G   +GE LY  R +  G L VGKV  SH C Y+P  S+E +I  YE+L 
Sbjct:    5 SWLHFSNGAIPQAAVVAGHDSDGDTIFIGRAFYCNDMLPAKIIPNKGKAYVAYANQEVELENYEVLS-------GFNYEWLPAENGEVPPGAVKVGQNVDGETLYAGRGYHAGSLTVGKVHPSHGCLYIPYDSEEVKIFAYEVLS 142
[Close]
BLAST of DUF3421 domain-containing protein vs. TrEMBL
Match: A0A4Z2D656 (DM9 domain-containing protein OS=Schistosoma japonicum OX=6182 GN=EWB00_004219 PE=4 SV=1)

HSP 1 Score: 144.436 bits (363), Expect = 7.342e-39
Identity = 79/175 (45.14%), Postives = 102/175 (58.29%), Query Frame = 2 
Query:   29 LSLLTFDSHARFLKYYKLNMSRAGKGRQACVSWLPGQYGQIPY-NAISAGDGVFIARAKTGSDVTPGMVRIGYNNAFFPFDGREEFITNYEVLCDTGLHVFGPEIVWKHMGGGCVPSGAIVGGNTSNGEPLYIARAHVNGELCVGKVCQSHKCAYMPNQSKEHRIQNYEILCFHE 550
             +LLT+       K  K+ M    K RQA +SWLPG  GQIP  +AI A  G+++ R +T  DV PG   I Y   +  +DGRE  ++N+EVLC+T L   G    W    GG VP  A+  G TS+ EPLY+AR  VNGE C+GKV  SH CAY P    EH ++ YE+LCF +
Sbjct:  134 FALLTYGG-----KELKMKMWPQNKTRQAHLSWLPGCAGQIPPPHAIEATPGIYVIRGRTCDDVIPGKWVINYGLGYISYDGREIELSNFEVLCNTSLQSTGNLYTWIPCSGGNVPDKALHAGETSSSEPLYVARGIVNGETCIGKVHPSHGCAYFPWGGDEHAVKCYEVLCFTD 303

HSP 2 Score: 88.5817 bits (218), Expect = 1.387e-17
Identity = 50/149 (33.56%), Postives = 80/149 (53.69%), Query Frame = 2 
Query:   86 MSRAGKGRQACVSWLPGQYGQIP-YNAISAGDGVFIARAKTGSDVTPGMVRIGYNNAFFPFDGREEFITNYEVLCDTGLHVFGPEIVWKHMGGGCVPSGAIVGGNTSNGEPLYIARAHVNGELCVGKVCQSHKCAYMPNQSKEHRIQNY 529
            M+ A   R+A +SW+P   G  P  N + A  G+ + R K G+++ PG + +     + P++G+E  I  YEVLC T +        W     G VP GA++ G T + E LY+ARA + GE+ +GK+  S K A +    KE +++ +
Sbjct:    1 MNVAHNAREANLSWIPYSTGSPPPSNIVQAESGICVIRGKKGTNLIPGKMLLKNGVGYIPYEGKEVQINEYEVLCQTNIFPHQTLYNWVPGSRGKVPVGALLAGITMHEELLYVARATIEGEMSIGKINCSQKFALLTYGGKELKMKMW 149
[Close]
BLAST of DUF3421 domain-containing protein vs. TrEMBL
Match: G4V7P2 (DUF3421 domain-containing protein OS=Schistosoma mansoni OX=6183 GN=Smp_035560.2 PE=4 SV=1)

HSP 1 Score: 139.813 bits (351), Expect = 8.329e-39
Identity = 73/155 (47.10%), Postives = 96/155 (61.94%), Query Frame = 2 
Query:   86 MSRAGKGRQACVSWLPGQYGQIPYNAISAGDGVFIARAKTGSDVTPGMVRIGYNNAFFPFDGREEFITNYEVLCDTGLHVFGPE--IVWKHMGGGCVPSGAIVGGNTSNGEPLYIARAHVNGELCVGKVCQSHKCAYMPNQSKEHRIQNYEILCF 544
            M+R GKG Q+C+SW+    G++P NAI AG  V+IAR     DV PG V      A+  +DG E    +YEVLCDT L  +GP+    W+    G VP  A+VGG TS+ EPLYIAR H+NGE  VGKV +SH CAY P   +E ++ +Y++L  
Sbjct:    1 MARIGKGIQSCLSWIHQTDGRVPSNAIDAGHDVYIARIYHSGDVIPGKVVPHLGKAYISYDGTEYEFDSYEVLCDTKL-PYGPQHCYKWERHSNGYVPKYAVVGGITSSNEPLYIAREHINGERVVGKVHESHGCAYFPYGGQERKMNHYDVLVL 154
[Close]
BLAST of DUF3421 domain-containing protein vs. TrEMBL
Match: A0A183RBG8 (DUF3421 domain-containing protein OS=Schistosoma rodhaini OX=6188 PE=4 SV=1)

HSP 1 Score: 140.198 bits (352), Expect = 9.789e-39
Identity = 76/171 (44.44%), Postives = 101/171 (59.06%), Query Frame = 2 
Query:   38 LTFDSHARFLKYYKLNMSRAGKGRQACVSWLPGQYGQIPYNAISAGDGVFIARAKTGSDVTPGMVRIGYNNAFFPFDGREEFITNYEVLCDTGLHVFGPE--IVWKHMGGGCVPSGAIVGGNTSNGEPLYIARAHVNGELCVGKVCQSHKCAYMPNQSKEHRIQNYEILCF 544
            L  D H+R     +  M+R GKG Q+C+SW+    G++P NAI AG  V+I R     DV PG V      A+  +DG E    +YEVLCDT L  +GP+    W+    G VP  A+VGG TS+ EPLYIAR ++NGE  VGKV +SH CAY P   +E ++ +YE+L  
Sbjct:    5 LRVDVHSRT----RTQMARIGKGIQSCLSWIHQTDGRVPTNAIDAGHAVYITRICHSGDVIPGKVVPHLGKAYISYDGTEYEFDSYEVLCDTKL-PYGPQHCYKWERHSNGYVPKYAVVGGITSSNEPLYIAREYINGERVVGKVHESHGCAYFPYGGQERKMNHYEVLVL 170
[Close]
BLAST of DUF3421 domain-containing protein vs. TrEMBL
Match: A0A095AJD7 (DUF3421 domain-containing protein OS=Schistosoma haematobium OX=6185 GN=MS3_02359 PE=4 SV=1)

HSP 1 Score: 139.428 bits (350), Expect = 1.070e-38
Identity = 72/155 (46.45%), Postives = 98/155 (63.23%), Query Frame = 2 
Query:   86 MSRAGKGRQACVSWLPGQYGQIPYNAISAGDGVFIARAKTGSDVTPGMVRIGYNNAFFPFDGREEFITNYEVLCDTGLHVFGPE--IVWKHMGGGCVPSGAIVGGNTSNGEPLYIARAHVNGELCVGKVCQSHKCAYMPNQSKEHRIQNYEILCF 544
            M+R GKG Q+C+SW+  + G++P NAI AG  V+IAR     D+ PG V    + A+  +DG E    +YEVLCDT L   GP+    W+    G VP  A+VGG TS+ EPLYIAR ++NGE  VGKV +SH+CAY P   +E ++ +YE+L  
Sbjct:    1 MARIGKGIQSCLSWIHQRDGRVPSNAIDAGHAVYIARIYHSGDLIPGKVVPHLSKAYVSYDGTEYEFDSYEVLCDTKL-PHGPQQCYKWERHSNGYVPKYAVVGGITSSNEPLYIAREYINGERVVGKVHESHECAYFPYGGQERKMHHYEVLVL 154
[Close]
BLAST of DUF3421 domain-containing protein vs. TrEMBL
Match: A0A430QF84 (DUF3421 domain-containing protein OS=Schistosoma bovis OX=6184 GN=DC041_0007183 PE=4 SV=1)

HSP 1 Score: 138.658 bits (348), Expect = 2.145e-38
Identity = 72/155 (46.45%), Postives = 97/155 (62.58%), Query Frame = 2 
Query:   86 MSRAGKGRQACVSWLPGQYGQIPYNAISAGDGVFIARAKTGSDVTPGMVRIGYNNAFFPFDGREEFITNYEVLCDTGLHVFGPE--IVWKHMGGGCVPSGAIVGGNTSNGEPLYIARAHVNGELCVGKVCQSHKCAYMPNQSKEHRIQNYEILCF 544
            M+R GKG Q+C+SW+  + G++P NAI AG  V+IAR     D+ PG V      A+  +DG E    +YEVLCDT L   GP+    W+    G VP  A+VGG TS+ EPLYIAR ++NGE  VGKV +SH+CAY P   +E ++ +YE+L  
Sbjct:    1 MARIGKGIQSCLSWIHQRDGRVPSNAIDAGHAVYIARIYHSGDLIPGKVVPHLGKAYVSYDGTEYEFDSYEVLCDTKL-PHGPQQCYKWERHSNGYVPKYAVVGGITSSNEPLYIAREYINGERVVGKVHESHECAYFPYGGQERKMHHYEVLVL 154
[Close]
BLAST of DUF3421 domain-containing protein vs. Planmine SMEST
Match: SMESG000046728.1 (SMESG000046728.1)

HSP 1 Score: 325.479 bits (833), Expect = 1.340e-115
Identity = 155/155 (100.00%), Postives = 155/155 (100.00%), Query Frame = 2 
Query:   86 MSRAGKGRQACVSWLPGQYGQIPYNAISAGDGVFIARAKTGSDVTPGMVRIGYNNAFFPFDGREEFITNYEVLCDTGLHVFGPEIVWKHMGGGCVPSGAIVGGNTSNGEPLYIARAHVNGELCVGKVCQSHKCAYMPNQSKEHRIQNYEILCFHE 550
            MSRAGKGRQACVSWLPGQYGQIPYNAISAGDGVFIARAKTGSDVTPGMVRIGYNNAFFPFDGREEFITNYEVLCDTGLHVFGPEIVWKHMGGGCVPSGAIVGGNTSNGEPLYIARAHVNGELCVGKVCQSHKCAYMPNQSKEHRIQNYEILCFHE
Sbjct:    1 MSRAGKGRQACVSWLPGQYGQIPYNAISAGDGVFIARAKTGSDVTPGMVRIGYNNAFFPFDGREEFITNYEVLCDTGLHVFGPEIVWKHMGGGCVPSGAIVGGNTSNGEPLYIARAHVNGELCVGKVCQSHKCAYMPNQSKEHRIQNYEILCFHE 155
[Close]
BLAST of DUF3421 domain-containing protein vs. Planmine SMEST
Match: SMESG000046742.1 (SMESG000046742.1)

HSP 1 Score: 219.55 bits (558), Expect = 8.561e-74
Identity = 97/153 (63.40%), Postives = 120/153 (78.43%), Query Frame = 2 
Query:   86 MSRAGKGRQACVSWLPGQYGQIPYNAISAGDGVFIARAKTGSDVTPGMVRIGYNNAFFPFDGREEFITNYEVLCDTGLHVFGPEIVWKHMGGGCVPSGAIVGGNTSNGEPLYIARAHVNGELCVGKVCQSHKCAYMPNQSKEHRIQNYEILCF 544
            M   G GRQA +SW PG  GQ+PYNAI A  G+FI RA+TG DVTPG +++GYN  +F + G+EE I+NYEVLCDTGLHVFGPE +W HM  GCVP  A+VGG+TS+ EPLYI RA ++G++CVGK+CQSH CAYMP   +EHRI NYE+LC+
Sbjct:    1 MCSVGSGRQASLSWQPGNNGQVPYNAIQADGGLFIGRARTGGDVTPGKLKVGYNTGYFSYAGKEECISNYEVLCDTGLHVFGPETIWIHMSNGCVPPNALVGGHTSSNEPLYIGRARISGDMCVGKICQSHGCAYMPYGGQEHRINNYEVLCY 153
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BLAST of DUF3421 domain-containing protein vs. Planmine SMEST
Match: SMESG000046710.1 (SMESG000046710.1)

HSP 1 Score: 215.698 bits (548), Expect = 2.720e-72
Identity = 95/155 (61.29%), Postives = 122/155 (78.71%), Query Frame = 2 
Query:   86 MSRAGKGRQACVSWLPGQYGQIPYNAISAGDGVFIARAKTGSDVTPGMVRIGYNNAFFPFDGREEFITNYEVLCDTGLHVFGPEIVWKHMGGGCVPSGAIVGGNTSNGEPLYIARAHVNGELCVGKVCQSHKCAYMPNQSKEHRIQNYEILCFHE 550
            MSRAG GRQA +SW PGQYGQ+PYNAI A  G++I R +TG D+T G  ++G+N+ +FP+ GRE+ I+NYEVLCDTGLHVFG E  W+HM  G VP  A+VGG+T++ EPLYIA+A +NGE+CVGKVCQSH CA+MP    EH+  +YE+LCF +
Sbjct:    1 MSRAGSGRQASLSWQPGQYGQVPYNAIQADAGLYIVRTRTGGDLTIGKTKVGHNSGYFPYAGREDSISNYEVLCDTGLHVFGGEHHWQHMANGSVPHNAVVGGHTASNEPLYIAKARINGEMCVGKVCQSHGCAFMPYGGAEHKAHHYEVLCFSD 155
[Close]
BLAST of DUF3421 domain-containing protein vs. Planmine SMEST
Match: SMESG000046697.1 (SMESG000046697.1)

HSP 1 Score: 215.698 bits (548), Expect = 2.720e-72
Identity = 95/155 (61.29%), Postives = 122/155 (78.71%), Query Frame = 2 
Query:   86 MSRAGKGRQACVSWLPGQYGQIPYNAISAGDGVFIARAKTGSDVTPGMVRIGYNNAFFPFDGREEFITNYEVLCDTGLHVFGPEIVWKHMGGGCVPSGAIVGGNTSNGEPLYIARAHVNGELCVGKVCQSHKCAYMPNQSKEHRIQNYEILCFHE 550
            MSRAG GRQA +SW PGQYGQ+PYNAI A  G++I R +TG D+T G  ++G+N+ +FP+ GRE+ I+NYEVLCDTGLHVFG E  W+HM  G VP  A+VGG+T++ EPLYIA+A +NGE+CVGKVCQSH CA+MP    EH+  +YE+LCF +
Sbjct:    1 MSRAGSGRQASLSWQPGQYGQVPYNAIQADAGLYIVRTRTGGDLTIGKTKVGHNSGYFPYAGREDSISNYEVLCDTGLHVFGGEHHWQHMANGSVPHNAVVGGHTASNEPLYIAKARINGEMCVGKVCQSHGCAFMPYGGAEHKAHHYEVLCFSD 155
[Close]
BLAST of DUF3421 domain-containing protein vs. Planmine SMEST
Match: SMESG000057816.1 (SMESG000057816.1)

HSP 1 Score: 202.601 bits (514), Expect = 3.934e-67
Identity = 89/155 (57.42%), Postives = 117/155 (75.48%), Query Frame = 2 
Query:   86 MSRAGKGRQACVSWLPGQYGQIPYNAISAGDGVFIARAKTGSDVTPGMVRIGYNNAFFPFDGREEFITNYEVLCDTGLHVFGPEIVWKHMGGGCVPSGAIVGGNTSNGEPLYIARAHVNGELCVGKVCQSHKCAYMPNQSKEHRIQNYEILCFHE 550
            MSRAG GRQ  +SWLPG+YGQ+P NA+ A  G++I RA+T  DVT G  ++G+ + FFP+ GRE+ +  YEVLCDTGLHV+G E  W++MG G VP  A+VGG+TS  EPLYIARA ++GE+CVGKV  SHKC +MP   KEH++  YE+LC+ +
Sbjct:    1 MSRAGTGRQISLSWLPGEYGQVPPNAVQADPGLYIVRARTDGDVTVGKTKVGHQSGFFPYAGREDMVPRYEVLCDTGLHVWGSEFHWQYMGNGAVPHNAVVGGHTSTYEPLYIARARIHGEMCVGKVANSHKCGFMPYGGKEHKVYKYEVLCYRD 155
The following BLAST results are available for this feature:
BLAST of DUF3421 domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DUF3421 domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DUF3421 domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CG133212.461e-2238.13gene:FBgn0033787 transcript:FBtr0345371[more]
CG133212.461e-2238.13gene:FBgn0033787 transcript:FBtr0087792[more]
CG105272.330e-1934.04gene:FBgn0034583 transcript:FBtr0071541[more]
CG310861.340e-1836.55gene:FBgn0051086 transcript:FBtr0084989[more]
CG310861.340e-1836.55gene:FBgn0051086 transcript:FBtr0307341[more]
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BLAST of DUF3421 domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DUF3421 domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DUF3421 domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DUF3421 domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DUF3421 domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A4Z2D6567.342e-3945.14DM9 domain-containing protein OS=Schistosoma japon... [more]
G4V7P28.329e-3947.10DUF3421 domain-containing protein OS=Schistosoma m... [more]
A0A183RBG89.789e-3944.44DUF3421 domain-containing protein OS=Schistosoma r... [more]
A0A095AJD71.070e-3846.45DUF3421 domain-containing protein OS=Schistosoma h... [more]
A0A430QF842.145e-3846.45DUF3421 domain-containing protein OS=Schistosoma b... [more]
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BLAST of DUF3421 domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DUF3421 domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DUF3421 domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DUF3421 domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DUF3421 domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DUF3421 domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000046728.11.340e-115100.00SMESG000046728.1[more]
SMESG000046742.18.561e-7463.40SMESG000046742.1[more]
SMESG000046710.12.720e-7261.29SMESG000046710.1[more]
SMESG000046697.12.720e-7261.29SMESG000046697.1[more]
SMESG000057816.13.934e-6757.42SMESG000057816.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30020231 ID=SMED30020231|Name=DUF3421 domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=573bp
TGTTTGGGCACCCTGAAAATTTATATTTTTGTCTTTACTGACCTTTGATT
CGCACGCTAGATTTCTCAAATATTATAAATTAAACATGTCTAGAGCTGGA
AAAGGACGTCAAGCATGCGTATCATGGCTACCAGGACAATACGGCCAGAT
ACCTTACAACGCCATTTCTGCTGGGGATGGAGTATTTATTGCACGCGCCA
AAACCGGAAGCGACGTGACTCCTGGAATGGTACGAATAGGTTATAATAAT
GCATTTTTTCCATTTGATGGAAGGGAAGAATTCATAACCAATTACGAAGT
TTTATGCGACACTGGACTCCATGTGTTTGGCCCTGAGATTGTTTGGAAAC
ATATGGGTGGAGGATGCGTTCCTTCTGGTGCAATTGTTGGAGGAAATACT
TCAAATGGAGAACCCTTATATATTGCCAGAGCTCATGTTAATGGGGAGTT
GTGTGTTGGAAAGGTTTGCCAATCTCACAAATGTGCATATATGCCAAATC
AGTCCAAGGAACATCGAATTCAAAATTATGAAATTTTATGTTTCCATGAA
TAAATTTTTTTCTTAACTAAAAA
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protein sequence of SMED30020231-orf-1

>SMED30020231-orf-1 ID=SMED30020231-orf-1|Name=SMED30020231-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=156bp
MSRAGKGRQACVSWLPGQYGQIPYNAISAGDGVFIARAKTGSDVTPGMVR
IGYNNAFFPFDGREEFITNYEVLCDTGLHVFGPEIVWKHMGGGCVPSGAI
VGGNTSNGEPLYIARAHVNGELCVGKVCQSHKCAYMPNQSKEHRIQNYEI
LCFHE*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0002075pigment cell
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR006616DM9_repeat
IPR024518DUF3421
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006616DM9 repeatSMARTSM00696dm9coord: 11..76
e-value: 1.0E-5
score: 27.5
coord: 84..154
e-value: 9.5E-21
score: 84.9
IPR024518Domain of unknown function DUF3421PFAMPF11901DUF3421coord: 30..144
e-value: 3.1E-31
score: 107.7
NoneNo IPR availablePANTHERPTHR31649FAMILY NOT NAMEDcoord: 10..75
coord: 84..154