39S ribosomal protein L49 mitochondrial

Overview
Name39S ribosomal protein L49 mitochondrial
Smed IDSMED30020227
Length (bp)616
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of 39S ribosomal protein L49 mitochondrial (SMED30020227) t-SNE clustered cells

Violin plots show distribution of expression levels for 39S ribosomal protein L49 mitochondrial (SMED30020227) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of 39S ribosomal protein L49 mitochondrial (SMED30020227) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for 39S ribosomal protein L49 mitochondrial (SMED30020227) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30020227

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30020227SMESG000017394.1 SmedASXL_001304SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of 39S ribosomal protein L49 mitochondrial vs. Ensembl Human
Match: MRPL49 (mitochondrial ribosomal protein L49 [Source:HGNC Symbol;Acc:HGNC:1176])

HSP 1 Score: 58.5362 bits (140), Expect = 9.383e-11
Identity = 29/91 (31.87%), Postives = 52/91 (57.14%), Query Frame = 2 
Query:  302 HLPYQIARNRNHSLPVYLKEVLRHYAEKAGGKRELTEVKKISGNIWKLGAELKSLLEPIADGPVIMQVHEPGCQIKINGNFVEEICEFLLE 574
            +LPY + R+R H++PV        Y +   G R++T ++K+ G+IW L  +++  L P+     + QV+E    ++I G F +E+  +LLE
Sbjct:   42 NLPYFVRRSRMHNIPV--------YKDITHGNRQMTVIRKVEGDIWALQKDVEDFLSPLLGKTPVTQVNEVTGTLRIKGYFDQELKAWLLE 124
[Close]
BLAST of 39S ribosomal protein L49 mitochondrial vs. Ensembl Human
Match: MRPL49 (mitochondrial ribosomal protein L49 [Source:HGNC Symbol;Acc:HGNC:1176])

HSP 1 Score: 56.9954 bits (136), Expect = 8.512e-10
Identity = 40/126 (31.75%), Postives = 67/126 (53.17%), Query Frame = 2 
Query:  197 VYKYLPLATVPRPLKLLDGEATVSGWIAPKPEKTAHLPYQIARNRNHSLPVYLKEVLRHYAEKAGGKRELTEVKKISGNIWKLGAELKSLLEPIADGPVIMQVHEPGCQIKINGNFVEEICEFLLE 574
            V + LP   +P P K  +   T SGW  P  +   +LPY + R+R H++PV        Y +   G R++T ++K+ G+IW L  +++  L P+     + QV+E    ++I G F +E+  +LLE
Sbjct:   48 VERLLPATRIPDPPKH-EHYPTPSGW-QPPRDPPPNLPYFVRRSRMHNIPV--------YKDITHGNRQMTVIRKVEGDIWALQKDVEDFLSPLLGKTPVTQVNEVTGTLRIKGYFDQELKAWLLE 163
[Close]
BLAST of 39S ribosomal protein L49 mitochondrial vs. Ensembl Human
Match: MRPL49 (mitochondrial ribosomal protein L49 [Source:HGNC Symbol;Acc:HGNC:1176])

HSP 1 Score: 56.9954 bits (136), Expect = 8.512e-10
Identity = 40/126 (31.75%), Postives = 67/126 (53.17%), Query Frame = 2 
Query:  197 VYKYLPLATVPRPLKLLDGEATVSGWIAPKPEKTAHLPYQIARNRNHSLPVYLKEVLRHYAEKAGGKRELTEVKKISGNIWKLGAELKSLLEPIADGPVIMQVHEPGCQIKINGNFVEEICEFLLE 574
            V + LP   +P P K  +   T SGW  P  +   +LPY + R+R H++PV        Y +   G R++T ++K+ G+IW L  +++  L P+     + QV+E    ++I G F +E+  +LLE
Sbjct:   48 VERLLPATRIPDPPKH-EHYPTPSGW-QPPRDPPPNLPYFVRRSRMHNIPV--------YKDITHGNRQMTVIRKVEGDIWALQKDVEDFLSPLLGKTPVTQVNEVTGTLRIKGYFDQELKAWLLE 163
[Close]
BLAST of 39S ribosomal protein L49 mitochondrial vs. Ensembl Fly
Match: mRpL49 (gene:FBgn0030433 transcript:FBtr0073691)

HSP 1 Score: 70.4774 bits (171), Expect = 4.758e-15
Identity = 42/127 (33.07%), Postives = 72/127 (56.69%), Query Frame = 2 
Query:  197 VYKYLPLATVPRPLKLLDGEATVSGWIAPKPEKTAHLPYQIARNRNHSLPVYLKEVLRHYAEKAGGKRELTEVKKISGNIWKLGAELKSLLEPIADGPVI-MQVHEPGCQIKINGNFVEEICEFLLE 574
            V + LP  TVP+P+   +     SGW  P+ E  + L Y +AR +NH +PVYL    R       G+R +T V+++ G+IW L  +L++++E   +G V   +++E   QI  +G+ V+ + ++L E
Sbjct:   61 VERLLPAKTVPQPV---EKPKYPSGW-QPQKEDGSELGYHVARTKNHMVPVYLHTRFR-------GQRRITVVRRVQGDIWTLEKDLRAVVEQSRNGKVCATRINELSGQIHFHGDHVDVLRDYLKE 176
[Close]
BLAST of 39S ribosomal protein L49 mitochondrial vs. Ensembl Fly
Match: mRpL49 (gene:FBgn0030433 transcript:FBtr0343185)

HSP 1 Score: 70.4774 bits (171), Expect = 4.758e-15
Identity = 42/127 (33.07%), Postives = 72/127 (56.69%), Query Frame = 2 
Query:  197 VYKYLPLATVPRPLKLLDGEATVSGWIAPKPEKTAHLPYQIARNRNHSLPVYLKEVLRHYAEKAGGKRELTEVKKISGNIWKLGAELKSLLEPIADGPVI-MQVHEPGCQIKINGNFVEEICEFLLE 574
            V + LP  TVP+P+   +     SGW  P+ E  + L Y +AR +NH +PVYL    R       G+R +T V+++ G+IW L  +L++++E   +G V   +++E   QI  +G+ V+ + ++L E
Sbjct:   61 VERLLPAKTVPQPV---EKPKYPSGW-QPQKEDGSELGYHVARTKNHMVPVYLHTRFR-------GQRRITVVRRVQGDIWTLEKDLRAVVEQSRNGKVCATRINELSGQIHFHGDHVDVLRDYLKE 176
[Close]
BLAST of 39S ribosomal protein L49 mitochondrial vs. Ensembl Zebrafish
Match: MRPL49 (mitochondrial ribosomal protein L49 [Source:NCBI gene;Acc:558658])

HSP 1 Score: 62.3882 bits (150), Expect = 6.098e-12
Identity = 45/130 (34.62%), Postives = 68/130 (52.31%), Query Frame = 2 
Query:  182 ELFDRVYKYLPLATVPRPLKLLDGEATVSGWIAPKPEKTAHLPYQIARNRNHSLPVYLKEVLRHYAEKAGGKRELTEVKKISGNIWKLGAELKSLLEPIADGPVIMQVHEPGCQIKINGNFVEEICEFLL 571
            E F  V + +P + +P P K  DG A  SGW  P  +    LPY I R+R H++PVY    ++H      G +  T ++KI G+IW L  E+K  L  +       QV+E    I++ G F +E+ ++LL
Sbjct:   54 EEFRFVERLIPPSRIPAPPKH-DGPAP-SGWTPPS-DTPPSLPYMIRRSRMHNVPVYSD--IKH------GNQHSTLIRKIEGDIWALNKEVKEFLLELTGKDPPTQVNEITGSIRVKGQFDKELKDWLL 172
[Close]
BLAST of 39S ribosomal protein L49 mitochondrial vs. Ensembl Zebrafish
Match: BX248510.5 (mitochondrial ribosomal protein L49 [Source:NCBI gene;Acc:558658])

HSP 1 Score: 62.3882 bits (150), Expect = 6.098e-12
Identity = 45/130 (34.62%), Postives = 68/130 (52.31%), Query Frame = 2 
Query:  182 ELFDRVYKYLPLATVPRPLKLLDGEATVSGWIAPKPEKTAHLPYQIARNRNHSLPVYLKEVLRHYAEKAGGKRELTEVKKISGNIWKLGAELKSLLEPIADGPVIMQVHEPGCQIKINGNFVEEICEFLL 571
            E F  V + +P + +P P K  DG A  SGW  P  +    LPY I R+R H++PVY    ++H      G +  T ++KI G+IW L  E+K  L  +       QV+E    I++ G F +E+ ++LL
Sbjct:   54 EEFRFVERLIPPSRIPAPPKH-DGPAP-SGWTPPS-DTPPSLPYMIRRSRMHNVPVYSD--IKH------GNQHSTLIRKIEGDIWALNKEVKEFLLELTGKDPPTQVNEITGSIRVKGQFDKELKDWLL 172
[Close]
BLAST of 39S ribosomal protein L49 mitochondrial vs. Ensembl Zebrafish
Match: BX088530.1 (mitochondrial ribosomal protein L49 [Source:NCBI gene;Acc:558658])

HSP 1 Score: 62.003 bits (149), Expect = 8.374e-12
Identity = 45/131 (34.35%), Postives = 68/131 (51.91%), Query Frame = 2 
Query:  182 ELFDRVYKYLPLATVPRPLKLLDGEATVSGWIAPKPEKTAHLPYQIARNRNHSLPVYLKEVLRHYAEKAGGKRELTEVKKISGNIWKLGAELKSLLEPIADGPVIMQVHEPGCQIKINGNFVEEICEFLLE 574
            E F  V + +P + +P P K  DG A  SGW  P  +    LPY I R+R H++PVY    ++H      G +  T ++KI G+IW L  E+K  L  +       QV+E    I++ G F +E+ ++LL 
Sbjct:   54 EEFRFVERLIPPSRIPAPPKH-DGPAP-SGWTPPS-DTPPSLPYMIRRSRMHNVPVYSD--IKH------GNQHSTLIRKIEGDIWALNKEVKEFLLGLTGKDPPTQVNEITGSIRVKGQFDKELKDWLLN 173
[Close]
BLAST of 39S ribosomal protein L49 mitochondrial vs. Ensembl Xenopus
Match: MRPL49 (mitochondrial ribosomal protein L49 [Source:NCBI gene;Acc:100486190])

HSP 1 Score: 62.7734 bits (151), Expect = 4.881e-12
Identity = 44/126 (34.92%), Postives = 63/126 (50.00%), Query Frame = 2 
Query:  197 VYKYLPLATVPRPLKLLDGEATVSGWIAPKPEKTAHLPYQIARNRNHSLPVYLKEVLRHYAEKAGGKRELTEVKKISGNIWKLGAELKSLLEPIADGPVIMQVHEPGCQIKINGNFVEEICEFLLE 574
            V + +P   VP P K      T SGWI PK E    LPY + R+R H++PV        Y +   G R +T V+KI G+IW L  E+++ L  +       QV+E    I+I G   +E+  +L E
Sbjct:   48 VERLIPPTQVPNPPK--HSAPTPSGWIPPK-ELPPQLPYFVRRSRMHNIPV--------YTDITHGNRHMTVVRKIEGDIWALELEVRNFLTELTGKTPPTQVNEISRTIRIKGYHDKELRTWLAE 162
[Close]
BLAST of 39S ribosomal protein L49 mitochondrial vs. Ensembl Mouse
Match: Mrpl49 (mitochondrial ribosomal protein L49 [Source:MGI Symbol;Acc:MGI:108180])

HSP 1 Score: 66.2402 bits (160), Expect = 2.120e-13
Identity = 42/129 (32.56%), Postives = 67/129 (51.94%), Query Frame = 2 
Query:  188 FDRVYKYLPLATVPRPLKLLDGEATVSGWIAPKPEKTAHLPYQIARNRNHSLPVYLKEVLRHYAEKAGGKRELTEVKKISGNIWKLGAELKSLLEPIADGPVIMQVHEPGCQIKINGNFVEEICEFLLE 574
            +  V + LP   +P P K      T SGW  P+ +    LPY + R+R H++PV        Y E   G R++T ++K+ G+IW L  +++  L P+     I QV+E    ++I G F E++  +LLE
Sbjct:   45 YQFVERLLPPTKIPEPPKH-KHYPTPSGWQPPR-DPLPSLPYFVRRSRMHNIPV--------YKEITHGNRQMTLIRKVEGDIWALQKDVEEFLSPLLGKTPITQVNEVTGTLRIKGYFDEQLKAWLLE 163
[Close]
BLAST of 39S ribosomal protein L49 mitochondrial vs. Ensembl Mouse
Match: Mrpl49 (mitochondrial ribosomal protein L49 [Source:MGI Symbol;Acc:MGI:108180])

HSP 1 Score: 46.595 bits (109), Expect = 1.256e-6
Identity = 36/104 (34.62%), Postives = 54/104 (51.92%), Query Frame = 2 
Query:  188 FDRVYKYLPLATVPRPLKLLDGEATVSGWIAPKPEKTAHLPYQIARNRNHSLPVYLKEVLRHYAEKAGGKRELTEVKKISGNIWKLGAELKSLLEPI-ADGPVI 496
            +  V + LP   +P P K      T SGW  P+ +    LPY + R+R H++PVY KE+         G R++T ++K+ G+IW  G  +   L  + A GPVI
Sbjct:   19 YQFVERLLPPTKIPEPPKHKH-YPTPSGWQPPR-DPLPSLPYFVRRSRMHNIPVY-KEITH-------GNRQMTLIRKVEGDIWVSGLGVYPNLPGMKAWGPVI 112
[Close]
BLAST of 39S ribosomal protein L49 mitochondrial vs. UniProt/SwissProt
Match: sp|Q9VYI3|RM49_DROME (Probable 39S ribosomal protein L49, mitochondrial OS=Drosophila melanogaster OX=7227 GN=mRpL49 PE=3 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 4.770e-14
Identity = 42/127 (33.07%), Postives = 72/127 (56.69%), Query Frame = 2 
Query:  197 VYKYLPLATVPRPLKLLDGEATVSGWIAPKPEKTAHLPYQIARNRNHSLPVYLKEVLRHYAEKAGGKRELTEVKKISGNIWKLGAELKSLLEPIADGPVI-MQVHEPGCQIKINGNFVEEICEFLLE 574
            V + LP  TVP+P+   +     SGW  P+ E  + L Y +AR +NH +PVYL    R       G+R +T V+++ G+IW L  +L++++E   +G V   +++E   QI  +G+ V+ + ++L E
Sbjct:   61 VERLLPAKTVPQPV---EKPKYPSGW-QPQKEDGSELGYHVARTKNHMVPVYLHTRFR-------GQRRITVVRRVQGDIWTLEKDLRAVVEQSRNGKVCATRINELSGQIHFHGDHVDVLRDYLKE 176
[Close]
BLAST of 39S ribosomal protein L49 mitochondrial vs. UniProt/SwissProt
Match: sp|Q9CQ40|RM49_MOUSE (39S ribosomal protein L49, mitochondrial OS=Mus musculus OX=10090 GN=Mrpl49 PE=1 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 1.483e-12
Identity = 42/129 (32.56%), Postives = 67/129 (51.94%), Query Frame = 2 
Query:  188 FDRVYKYLPLATVPRPLKLLDGEATVSGWIAPKPEKTAHLPYQIARNRNHSLPVYLKEVLRHYAEKAGGKRELTEVKKISGNIWKLGAELKSLLEPIADGPVIMQVHEPGCQIKINGNFVEEICEFLLE 574
            +  V + LP   +P P K      T SGW  P+ +    LPY + R+R H++PV        Y E   G R++T ++K+ G+IW L  +++  L P+     I QV+E    ++I G F E++  +LLE
Sbjct:   45 YQFVERLLPPTKIPEPPKH-KHYPTPSGWQPPR-DPLPSLPYFVRRSRMHNIPV--------YKEITHGNRQMTLIRKVEGDIWALQKDVEEFLSPLLGKTPITQVNEVTGTLRIKGYFDEQLKAWLLE 163
[Close]
BLAST of 39S ribosomal protein L49 mitochondrial vs. UniProt/SwissProt
Match: sp|Q13405|RM49_HUMAN (39S ribosomal protein L49, mitochondrial OS=Homo sapiens OX=9606 GN=MRPL49 PE=1 SV=1)

HSP 1 Score: 56.9954 bits (136), Expect = 4.088e-9
Identity = 40/126 (31.75%), Postives = 67/126 (53.17%), Query Frame = 2 
Query:  197 VYKYLPLATVPRPLKLLDGEATVSGWIAPKPEKTAHLPYQIARNRNHSLPVYLKEVLRHYAEKAGGKRELTEVKKISGNIWKLGAELKSLLEPIADGPVIMQVHEPGCQIKINGNFVEEICEFLLE 574
            V + LP   +P P K  +   T SGW  P  +   +LPY + R+R H++PV        Y +   G R++T ++K+ G+IW L  +++  L P+     + QV+E    ++I G F +E+  +LLE
Sbjct:   48 VERLLPATRIPDPPKH-EHYPTPSGW-QPPRDPPPNLPYFVRRSRMHNIPV--------YKDITHGNRQMTVIRKVEGDIWALQKDVEDFLSPLLGKTPVTQVNEVTGTLRIKGYFDQELKAWLLE 163
[Close]
BLAST of 39S ribosomal protein L49 mitochondrial vs. UniProt/SwissProt
Match: sp|Q5R8X0|RM49_PONAB (39S ribosomal protein L49, mitochondrial OS=Pongo abelii OX=9601 GN=MRPL49 PE=2 SV=1)

HSP 1 Score: 56.9954 bits (136), Expect = 4.344e-9
Identity = 40/129 (31.01%), Postives = 68/129 (52.71%), Query Frame = 2 
Query:  188 FDRVYKYLPLATVPRPLKLLDGEATVSGWIAPKPEKTAHLPYQIARNRNHSLPVYLKEVLRHYAEKAGGKRELTEVKKISGNIWKLGAELKSLLEPIADGPVIMQVHEPGCQIKINGNFVEEICEFLLE 574
            +  V + LP   +P P K  +   T SGW  P  +   +LPY + R+R H++PV        Y +   G R++T ++K+ G+IW L  +++  L P+     + QV+E    ++I G F +E+  +LLE
Sbjct:   45 YQFVERLLPATRIPDPPKH-EHYPTPSGW-QPPRDPPPNLPYFVRRSRMHNIPV--------YKDITHGNRQMTVIRKVEGDIWALQKDVEDFLSPLLGKTPVTQVNEVTGTLRIKGYFDQELKAWLLE 163
[Close]
BLAST of 39S ribosomal protein L49 mitochondrial vs. UniProt/SwissProt
Match: sp|Q4R5K7|RM49_MACFA (39S ribosomal protein L49, mitochondrial OS=Macaca fascicularis OX=9541 GN=MRPL49 PE=2 SV=1)

HSP 1 Score: 56.6102 bits (135), Expect = 4.663e-9
Identity = 40/126 (31.75%), Postives = 67/126 (53.17%), Query Frame = 2 
Query:  197 VYKYLPLATVPRPLKLLDGEATVSGWIAPKPEKTAHLPYQIARNRNHSLPVYLKEVLRHYAEKAGGKRELTEVKKISGNIWKLGAELKSLLEPIADGPVIMQVHEPGCQIKINGNFVEEICEFLLE 574
            V + LP   +P P K  +   T SGW  P  +   +LPY + R+R H++PV        Y +   G R++T ++K+ G+IW L  +++  L P+     + QV+E    ++I G F +E+  +LLE
Sbjct:   48 VERLLPATRIPDPPKH-EHYPTPSGW-QPPRDPPPNLPYFVRRSRMHNVPV--------YKDITHGNRQMTVIRKVEGDIWALQKDVEDFLSPLLGKTPVTQVNEVTGTLRIKGYFDQELKAWLLE 163
[Close]
BLAST of 39S ribosomal protein L49 mitochondrial vs. TrEMBL
Match: G7Y370 (39S ribosomal protein L49 mitochondrial OS=Clonorchis sinensis OX=79923 GN=CLF_100321 PE=4 SV=1)

HSP 1 Score: 112.464 bits (280), Expect = 2.459e-27
Identity = 66/183 (36.07%), Postives = 99/183 (54.10%), Query Frame = 2 
Query:   50 IDAVRFSSQPTLWPTLKNPW-------VQELEKGEIKFDXXXXXXXXXXXXELFDRVYKYLPLATVPRPLKLLDGEATVSGWIAPKPEKTAHLPYQIARNRNHSLPVYLKEVLRHYAEKAGGKRELTEVKKISGNIWKLGAELKSLLEPIADGPVIM-QVHEPGCQIKINGNFVEEICEFLLE 574
            +  +++S     WP L+NPW         +L+  ++ FD S            F+ V K + +  +P P + +   AT SGW+ P PE + +  Y + R++NH LPVY KE  R  AE++ G R LT +K I G++W L  +L+ LL+P  DG + + QV E   QIKI G F+EE+  FLL 
Sbjct:   28 VSLIQYSINVRWWPNLQNPWEEASKSQSSKLQVPKVPFDISKSD---------FEWVRKIITINDIPLPNENIKA-ATPSGWVPPNPEISKNYSYSVRRSKNHMLPVYFKEKERKKAERSHGTRNLTIIKHIDGDMWALAEDLRQLLQPKCDGGLFLCQVDEATRQIKIEGIFLEEVSSFLLS 200
[Close]
BLAST of 39S ribosomal protein L49 mitochondrial vs. TrEMBL
Match: A0A3R7D7X4 (39S ribosomal protein L49 OS=Clonorchis sinensis OX=79923 GN=Mrpl49 PE=4 SV=1)

HSP 1 Score: 110.923 bits (276), Expect = 6.796e-27
Identity = 66/178 (37.08%), Postives = 97/178 (54.49%), Query Frame = 2 
Query:   62 RFSSQPTLWPTLKNPW-------VQELEKGEIKFDXXXXXXXXXXXXELFDRVYKYLPLATVPRPLKLLDGEATVSGWIAPKPEKTAHLPYQIARNRNHSLPVYLKEVLRHYAEKAGGKRELTEVKKISGNIWKLGAELKSLLEPIADGPVIM-QVHEPGCQIKINGNFVEEICEFLL 571
            ++S     WP L+NPW         +L+  ++ FD S            F+ V K + +  +P P + +   AT SGW+ P PE + +  Y + R++NH LPVY KE  R  AE++ G R LT +K I G++W L  +L+ LL+P  DG + + QV E   QIKI G F+EE+  FLL
Sbjct:   19 KYSINVRWWPNLQNPWEEASKSQSSKLQVPKVPFDISKSD---------FEWVRKIITINDIPLPNENIKA-ATPSGWVPPNPEISKNYSYSVRRSKNHMLPVYFKEKERKKAERSHGTRNLTIIKHIDGDMWALAEDLRQLLQPKCDGGLFLCQVDEATRQIKIEGIFLEEVSSFLL 186
[Close]
BLAST of 39S ribosomal protein L49 mitochondrial vs. TrEMBL
Match: A0A074ZDA3 (Uncharacterized protein OS=Opisthorchis viverrini OX=6198 GN=T265_07255 PE=4 SV=1)

HSP 1 Score: 108.227 bits (269), Expect = 7.271e-26
Identity = 63/178 (35.39%), Postives = 99/178 (55.62%), Query Frame = 2 
Query:   62 RFSSQPTLWPTLKNPW-------VQELEKGEIKFDXXXXXXXXXXXXELFDRVYKYLPLATVPRPLKLLDGEATVSGWIAPKPEKTAHLPYQIARNRNHSLPVYLKEVLRHYAEKAGGKRELTEVKKISGNIWKLGAELKSLLEPIADGPVIM-QVHEPGCQIKINGNFVEEICEFLL 571
            ++S     WP L+NPW         +L+  ++ FD S          + F+ V K + +  +P P + ++  AT SGW+ P PE + +  Y + R++NH LPVY K+  R  AE++ G R LT ++ I G++W L  +L+ LL+P  DG + + QV E   QIK+ G F+EE+  FLL
Sbjct:   19 KYSINVRWWPNLQNPWEEASRSQSSKLQVPKVPFDISK---------DDFEWVRKVITINDIPLPNENINS-ATPSGWVPPNPEISKNYSYSVRRSKNHMLPVYYKQKERKKAERSHGTRNLTIIRHIDGDMWALAEDLRQLLQPKCDGGLFLCQVDEATRQIKMEGIFLEEVSSFLL 186
[Close]
BLAST of 39S ribosomal protein L49 mitochondrial vs. TrEMBL
Match: A0A5J4NCG7 (Large subunit ribosomal protein L49 (Fragment) OS=Paragonimus westermani OX=34504 GN=DEA37_0010250 PE=4 SV=1)

HSP 1 Score: 105.531 bits (262), Expect = 1.053e-24
Identity = 68/185 (36.76%), Postives = 104/185 (56.22%), Query Frame = 2 
Query:   26 FKLVKFIRIDAVRFSSQPTLWPTLKNPWVQ--ELEKGEIKFDXXXXXXXXXXXXELFDRVYKYLPLATVPRPLKLLDGEATVSGWIAPKPEKTAHLPYQIARNRNHSLPVYLKEVLRHYAEKAGGKRELTEVKKISGNIWKLGAELKSLLEPIADGPVIM-QVHEPGCQIKINGNFVEEICEFLL 571
            F +  +I    ++FS     WPTL+NPW +  + + GE +  K  Y+   S     F+ V K +    +P P + L    T SGW+ P PE + +  Y + R++NH LPVY KE  R  AE++ G R LT ++ I G++W L  +L++LL+P  +G + + QV E    IKI G F+EE+ +FLL
Sbjct:   21 FLISSYIFTIILQFSVNIRWWPTLQNPWEERSKFKPGETELPKIEYEISKSD----FEWVKKIISTGEIPLPNEGL-VTPTPSGWVPPNPELSKNCSYHVRRSKNHMLPVYYKEKPRKAAERSHGTRPLTVIRHIDGDMWALADDLRALLQPKCEGGLFLCQVDEATRVIKIEGIFLEEVSQFLL 200
[Close]
BLAST of 39S ribosomal protein L49 mitochondrial vs. TrEMBL
Match: A0A0R3UPZ3 (Uncharacterized protein OS=Mesocestoides corti OX=53468 GN=MCOS_LOCUS9934 PE=4 SV=1)

HSP 1 Score: 103.99 bits (258), Expect = 2.830e-24
Identity = 64/164 (39.02%), Postives = 93/164 (56.71%), Query Frame = 2 
Query:   86 WPTLKNPWVQELEKGEIKFDXXXXXXXXXXXXELFDRVYKYLPLATVPRPLKLLDGEATVSGWIAPKPEKTAHLPYQIARNRNHSLPVYLKEVLRHYAEKAGGKRELTEVKKISGNIWKLGAELKSLLEPIAD-GPVIMQVHEPGCQIKINGNFVEEICEFLLE 574
            WPTL NPW + L+  +    K  +   Y  S E F  V    P   +P P   +D   T SGW+ P+PE ++  PY + R+RNH LPVYL +  R   E+  G R LT + K+ G++W L ++L++LL+P  + G  + QV E   +I I+G F+EE+  FLLE
Sbjct:   30 WPTLDNPWRERLDVSK----KGTFPPNYVVSKEEFKFVEMLKPADLIPPPPAGVD--VTPSGWVPPRPELSSKKPYTVTRSRNHMLPVYLHQKGRKRREQTHGTRMLTVITKVGGDMWALASDLEALLKPKCEAGQFLCQVDEATQKIIIDGIFLEEVAAFLLE 187
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BLAST of 39S ribosomal protein L49 mitochondrial vs. Ensembl Cavefish
Match: MRPL49 (mitochondrial ribosomal protein L49 [Source:HGNC Symbol;Acc:HGNC:1176])

HSP 1 Score: 61.2326 bits (147), Expect = 1.434e-11
Identity = 43/131 (32.82%), Postives = 67/131 (51.15%), Query Frame = 2 
Query:  182 ELFDRVYKYLPLATVPRPLKLLDGEATVSGWIAPKPEKTAHLPYQIARNRNHSLPVYLKEVLRHYAEKAGGKRELTEVKKISGNIWKLGAELKSLLEPIADGPVIMQVHEPGCQIKINGNFVEEICEFLLE 574
            E +  V + +P   VP P K      T SGW  P  E   +LPY I R+R H++PVY        ++   G ++ T V+K+ G+IW L  ++K  L+ +       QV+E    I+I G F  E+ E+L++
Sbjct:   48 EEYKFVERLIPPTRVPTPPK--HEGPTPSGWTPPS-ETPPNLPYMIRRSRMHNVPVY--------SDIKHGNQKSTIVRKVEGDIWALDKDVKKYLQQLTGREPPTQVNEVTGSIRIKGEFDTELKEWLVK 167
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BLAST of 39S ribosomal protein L49 mitochondrial vs. Ensembl Cavefish
Match: MRPL49 (mitochondrial ribosomal protein L49 [Source:HGNC Symbol;Acc:HGNC:1176])

HSP 1 Score: 61.2326 bits (147), Expect = 1.606e-11
Identity = 44/131 (33.59%), Postives = 68/131 (51.91%), Query Frame = 2 
Query:  182 ELFDRVYKYLPLATVPRPLKLLDGEATVSGWIAPKPEKTAHLPYQIARNRNHSLPVYLKEVLRHYAEKAGGKRELTEVKKISGNIWKLGAELKSLLEPIADGPVIMQVHEPGCQIKINGNFVEEICEFLLE 574
            E +  V + +P   VP P K      T SGW  P  E   +LPY I R+R H++PVY    ++H      G ++ T V+K+ G+IW L  ++K  L+ +       QV+E    I+I G F  E+ E+L++
Sbjct:   51 EEYKFVERLIPPTRVPTPPK--HEGPTPSGWTPPS-ETPPNLPYMIRRSRMHNVPVYSD--IKH------GNQKSTIVRKVEGDIWALDKDVKKYLQQLTGREPPTQVNEVTGSIRIKGEFDTELKEWLVK 170
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BLAST of 39S ribosomal protein L49 mitochondrial vs. Ensembl Sea Lamprey
Match: MRPL49 (mitochondrial ribosomal protein L49 [Source:HGNC Symbol;Acc:HGNC:1176])

HSP 1 Score: 55.8398 bits (133), Expect = 1.365e-10
Identity = 44/129 (34.11%), Postives = 67/129 (51.94%), Query Frame = 2 
Query:  188 FDRVYKYLPLATVPRPLKLLDGEATVSGWIAPKPEKTAHLPYQIARNRNHSLPVYLKEVLRHYAEKAGGKRELTEVKKISGNIWKLGAELKSLLEPIADGPVIMQVHEPGCQIKINGNFVEEICEFLLE 574
            F  V + +P   VP P K  +   T SGW +P  +    LPY + R R H++PVY K           G RELT +  I G+IW L  E++ LL+ ++    +++V+E   +I I G F  ++  +LLE
Sbjct:   17 FHYVERLIPPLRVPTPPKH-ERYPTPSGW-SPPRDPPPALPYFVKRTRMHNIPVYQK--------MTHGNRELTVISNIEGDIWVLEKEVRELLKTLSGEEPLIRVNEVSMKITIRGYFDTQLKAWLLE 135
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BLAST of 39S ribosomal protein L49 mitochondrial vs. Ensembl Nematostella
Match: EDO33268 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SSQ9])

HSP 1 Score: 58.5362 bits (140), Expect = 9.910e-12
Identity = 36/103 (34.95%), Postives = 57/103 (55.34%), Query Frame = 2 
Query:  266 SGWIAPKPEKTAHLPYQIARNRNHSLPVYLKEVLRHYAEKAGGKRELTEVKKISGNIWKLGAELKSLLEPIADGPVIMQVHEPGCQIKINGNFVEEICEFLLE 574
            SGW+ P  E T  LP+ I R+R +++PVY          KAGG R+LT ++K+ G++  L   L+S L       +++Q +E   Q+KI GN+ + +   L E
Sbjct:    3 SGWVPPS-ENTPALPFSIRRSRLNNIPVYTD-------IKAGGTRQLTVIRKVKGDLEALEKLLRSRL----GDDLVVQRNELTGQVKIKGNYKDRVVTILKE 93
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BLAST of 39S ribosomal protein L49 mitochondrial vs. Ensembl Medaka
Match: MRPL49 (mitochondrial ribosomal protein L49 [Source:HGNC Symbol;Acc:HGNC:1176])

HSP 1 Score: 54.6842 bits (130), Expect = 3.164e-9
Identity = 38/129 (29.46%), Postives = 63/129 (48.84%), Query Frame = 2 
Query:  182 ELFDRVYKYLPLATVPRPLKLLDGEATVSGWIAPKPEKTAHLPYQIARNRNHSLPVYLKEVLRHYAEKAGGKRELTEVKKISGNIWKLGAELKSLLEPIADGPVIMQVHEPGCQIKINGNFVEEICEFL 568
            E F  V + +P + +P P K        SGW  P         Y I R+R H++PVY         +   G R++T V+K+ G+IW L  ++K  L+ +    +  QV+E    ++I G+F +E+  +L
Sbjct:   51 EEFVFVERLIPPSRIPDPPK--HAGPAPSGWTPPAETPPPLP-YMIRRSRMHNIPVY--------TDLTHGNRKMTLVRKVEGDIWALEKDVKQFLQELTGKDMPTQVNEVTMTLRIKGHFDQELKSWL 168
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BLAST of 39S ribosomal protein L49 mitochondrial vs. Planmine SMEST
Match: SMESG000017394.1 (SMESG000017394.1)

HSP 1 Score: 347.821 bits (891), Expect = 8.413e-124
Identity = 183/185 (98.92%), Postives = 183/185 (98.92%), Query Frame = 2 
Query:   20 MNFKLVKFIRIDAVRFSSQPTLWPTLKNPWVQELEKGEIKFDXXXXXXXXXXXXELFDRVYKYLPLATVPRPLKLLDGEATVSGWIAPKPEKTAHLPYQIARNRNHSLPVYLKEVLRHYAEKAGGKRELTEVKKISGNIWKLGAELKSLLEPIADGPVIMQVHEPGCQIKINGNFVEEICEFLLE 574
            MNFKLVKFIRIDAVRFSSQPTLWPTLKNPWVQELEKGEIKFDKSNYKYKYSTSSELFDRVYKYLPLATVPRP KLLDGEATVSGWIAPKPEKTAHLPYQIARNRNHSLPVYLKEVLRHYAEKAGGKRELTEVKKISGNIWKLGAELKSLLEPIADGPVIMQVHEPGCQIKINGNFVEEICEFL E
Sbjct:    1 MNFKLVKFIRIDAVRFSSQPTLWPTLKNPWVQELEKGEIKFDKSNYKYKYSTSSELFDRVYKYLPLATVPRPPKLLDGEATVSGWIAPKPEKTAHLPYQIARNRNHSLPVYLKEVLRHYAEKAGGKRELTEVKKISGNIWKLGAELKSLLEPIADGPVIMQVHEPGCQIKINGNFVEEICEFLFE 185
The following BLAST results are available for this feature:
BLAST of 39S ribosomal protein L49 mitochondrial vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 3
Match NameE-valueIdentityDescription
MRPL499.383e-1131.87mitochondrial ribosomal protein L49 [Source:HGNC S... [more]
MRPL498.512e-1031.75mitochondrial ribosomal protein L49 [Source:HGNC S... [more]
MRPL498.512e-1031.75mitochondrial ribosomal protein L49 [Source:HGNC S... [more]
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BLAST of 39S ribosomal protein L49 mitochondrial vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of 39S ribosomal protein L49 mitochondrial vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
mRpL494.758e-1533.07gene:FBgn0030433 transcript:FBtr0073691[more]
mRpL494.758e-1533.07gene:FBgn0030433 transcript:FBtr0343185[more]
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BLAST of 39S ribosomal protein L49 mitochondrial vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
MRPL496.098e-1234.62mitochondrial ribosomal protein L49 [Source:NCBI g... [more]
BX248510.56.098e-1234.62mitochondrial ribosomal protein L49 [Source:NCBI g... [more]
BX088530.18.374e-1234.35mitochondrial ribosomal protein L49 [Source:NCBI g... [more]
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BLAST of 39S ribosomal protein L49 mitochondrial vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
MRPL494.881e-1234.92mitochondrial ribosomal protein L49 [Source:NCBI g... [more]
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BLAST of 39S ribosomal protein L49 mitochondrial vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Mrpl492.120e-1332.56mitochondrial ribosomal protein L49 [Source:MGI Sy... [more]
Mrpl491.256e-634.62mitochondrial ribosomal protein L49 [Source:MGI Sy... [more]
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BLAST of 39S ribosomal protein L49 mitochondrial vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q9VYI3|RM49_DROME4.770e-1433.07Probable 39S ribosomal protein L49, mitochondrial ... [more]
sp|Q9CQ40|RM49_MOUSE1.483e-1232.5639S ribosomal protein L49, mitochondrial OS=Mus mu... [more]
sp|Q13405|RM49_HUMAN4.088e-931.7539S ribosomal protein L49, mitochondrial OS=Homo s... [more]
sp|Q5R8X0|RM49_PONAB4.344e-931.0139S ribosomal protein L49, mitochondrial OS=Pongo ... [more]
sp|Q4R5K7|RM49_MACFA4.663e-931.7539S ribosomal protein L49, mitochondrial OS=Macaca... [more]
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BLAST of 39S ribosomal protein L49 mitochondrial vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
G7Y3702.459e-2736.0739S ribosomal protein L49 mitochondrial OS=Clonorc... [more]
A0A3R7D7X46.796e-2737.0839S ribosomal protein L49 OS=Clonorchis sinensis O... [more]
A0A074ZDA37.271e-2635.39Uncharacterized protein OS=Opisthorchis viverrini ... [more]
A0A5J4NCG71.053e-2436.76Large subunit ribosomal protein L49 (Fragment) OS=... [more]
A0A0R3UPZ32.830e-2439.02Uncharacterized protein OS=Mesocestoides corti OX=... [more]
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BLAST of 39S ribosomal protein L49 mitochondrial vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
MRPL491.434e-1132.82mitochondrial ribosomal protein L49 [Source:HGNC S... [more]
MRPL491.606e-1133.59mitochondrial ribosomal protein L49 [Source:HGNC S... [more]
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BLAST of 39S ribosomal protein L49 mitochondrial vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
MRPL491.365e-1034.11mitochondrial ribosomal protein L49 [Source:HGNC S... [more]
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BLAST of 39S ribosomal protein L49 mitochondrial vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of 39S ribosomal protein L49 mitochondrial vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO332689.910e-1234.95Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of 39S ribosomal protein L49 mitochondrial vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
MRPL493.164e-929.46mitochondrial ribosomal protein L49 [Source:HGNC S... [more]
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BLAST of 39S ribosomal protein L49 mitochondrial vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000017394.18.413e-12498.92SMESG000017394.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30020227 ID=SMED30020227|Name=39S ribosomal protein L49 mitochondrial|organism=Schmidtea mediterranea sexual|type=transcript|length=616bp
CTAATTATCAATTTAAATTATGAATTTCAAATTGGTGAAATTTATTAGAA
TTGATGCTGTTCGATTTTCTTCTCAGCCAACTTTATGGCCAACTTTGAAA
AACCCGTGGGTACAGGAGTTGGAAAAAGGAGAAATTAAATTCGATAAATC
AAATTATAAATATAAATATAGCACATCAAGTGAATTATTTGACAGAGTTT
ATAAATATCTACCACTGGCTACTGTTCCTAGACCTCTGAAATTATTAGAT
GGAGAAGCAACTGTCAGTGGATGGATTGCTCCAAAACCGGAAAAAACTGC
ACATTTACCATATCAAATCGCTCGAAACCGAAATCATAGTTTACCTGTAT
ATTTGAAGGAAGTGCTTCGGCATTATGCAGAGAAAGCCGGAGGAAAACGA
GAACTCACTGAAGTGAAAAAGATTAGTGGAAATATTTGGAAACTCGGGGC
TGAATTGAAGTCACTTTTGGAACCGATAGCGGACGGCCCGGTAATCATGC
AAGTCCACGAGCCGGGATGTCAAATTAAAATCAATGGCAATTTTGTAGAA
GAAATATGTGAATTTTTGTTGGAAAAAGGCTTTTGAAATAAATTATTTTT
AGTTCTTAAAAAAAAA
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protein sequence of SMED30020227-orf-1

>SMED30020227-orf-1 ID=SMED30020227-orf-1|Name=SMED30020227-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=189bp
MNFKLVKFIRIDAVRFSSQPTLWPTLKNPWVQELEKGEIKFDKSNYKYKY
STSSELFDRVYKYLPLATVPRPLKLLDGEATVSGWIAPKPEKTAHLPYQI
ARNRNHSLPVYLKEVLRHYAEKAGGKRELTEVKKISGNIWKLGAELKSLL
EPIADGPVIMQVHEPGCQIKINGNFVEEICEFLLEKGF*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR036877SUI1_dom_sf
IPR007740Ribosomal_L49/IMG2
IPR001950SUI1
Vocabulary: biological process
TermDefinition
GO:0006412translation
GO:0006413translational initiation
Vocabulary: cellular component
TermDefinition
GO:0005840ribosome
GO:0005622intracellular
Vocabulary: molecular function
TermDefinition
GO:0003735structural constituent of ribosome
GO:0003743translation initiation factor activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:3.30.780.10coord: 45..188
e-value: 1.3E-31
score: 111.3
IPR007740Ribosomal protein L49/IMG2PFAMPF05046Img2coord: 96..188
e-value: 2.0E-19
score: 69.7
IPR007740Ribosomal protein L49/IMG2PANTHERPTHR13477MITOCHONDRIAL 39S RIBOSOMAL PROTEIN L49coord: 45..188
IPR036877SUI1 domain superfamilySUPERFAMILYSSF55159eIF1-likecoord: 112..188