Coiled-coil domain-containing protein

Overview
NameCoiled-coil domain-containing protein
Smed IDSMED30020166
Length (bp)1317
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Coiled-coil domain-containing protein (SMED30020166) t-SNE clustered cells

Violin plots show distribution of expression levels for Coiled-coil domain-containing protein (SMED30020166) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Coiled-coil domain-containing protein (SMED30020166) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Coiled-coil domain-containing protein (SMED30020166) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30020166

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 11

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30020166SMESG000005080.1 SmedASXL_014089SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
pharynxSMED30020166SMESG000005080.1 dd_Smed_v4_9816_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30020166SMESG000005080.1 dd_Smed_v4_9816_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30020166SMESG000005080.1 dd_Smed_v4_9816_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30020166SMESG000005080.1 dd_Smed_v4_9816_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30020166SMESG000005080.1 dd_Smed_v4_9816_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30020166SMESG000005080.1 dd_Smed_v4_9816_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		whole organism asexual adult colorimetric in situ hybridization evidence
Smed sexual biotypeSMED30020166SMESG000005080.1 Contig34321newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30020166SMESG000005080.1 Contig34321uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
head regionSMED30020166SMESG000005080.1 dd_Smed_v6_9816_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
epidermisSMED30020166SMESG000005080.1 dd_Smed_v4_9816_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Coiled-coil domain-containing protein vs. TrEMBL
Match: A0A4E0RDV3 (Coiled-coil domain-containing protein OS=Fasciola hepatica OX=6192 GN=D915_004566 PE=4 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 4.683e-8
Identity = 27/47 (57.45%), Postives = 33/47 (70.21%), Query Frame = 3 
Query:  114 QIPQINLNNFDSDAFYNCEYVLTSPTSLEACKNLNIKPAELLAEYDE 254
            ++P I+LNNFD   F    YVLTSP SLEAC N+NIKP +LL +  E
Sbjct:   10 EVPNIDLNNFDDPGFVGSRYVLTSPRSLEACANMNIKPVDLLPKSKE 56
[Close]
BLAST of Coiled-coil domain-containing protein vs. TrEMBL
Match: A0A504YZI1 (Uncharacterized protein OS=Fasciola gigantica OX=46835 GN=FGIG_12448 PE=4 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 1.086e-7
Identity = 27/47 (57.45%), Postives = 34/47 (72.34%), Query Frame = 3 
Query:  114 QIPQINLNNFDSDAFYNCEYVLTSPTSLEACKNLNIKPAELLAEYDE 254
            ++P I+LNNFD  +F    YVLTSP SLEAC N+NIKP +LL +  E
Sbjct:   10 EVPNIDLNNFDDPSFVASRYVLTSPRSLEACANMNIKPVDLLPKSKE 56
[Close]
BLAST of Coiled-coil domain-containing protein vs. TrEMBL
Match: A0A4S2L7D4 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_009465 PE=4 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 1.182e-7
Identity = 48/150 (32.00%), Postives = 84/150 (56.00%), Query Frame = 3 
Query:  708 EKKAINVSHRRKQIEDEILIYYRELERFQNIT---------IKSAEEKFQKKLQAEQNKIQYEREYKELVNRHNRELIVEQDTNRICETMVKIIDREKQNDMFLQEKLNKMIESRCLANEAQKVREEIRKIYNLDSFDRKAVQAEIVNQV 1130
            E++      RR+++E  +  Y REL+  + IT         ++S+EE+  +K+Q  Q + Q+ RE  +++ +   E        R  E M K   +E+    FL+E+  K+ ESR LA  A+++R E++++YNLDSFD+KA    +VN++
Sbjct:  264 ERRLRQAQERRRELEKMLETYRRELQEAREITHQRARERAYLRSSEEQL-RKVQDRQQREQHIRENAKIIQQRQEEW------RRAAEAMQK--QKEEHVQKFLEEREAKIQESRNLAQAAEQLRAEMQRVYNLDSFDKKAQHVALVNEL 404

HSP 2 Score: 63.1586 bits (152), Expect = 5.431e-7
Identity = 27/40 (67.50%), Postives = 29/40 (72.50%), Query Frame = 3 
Query:  120 PQINLNNFDSDAFYNCEYVLTSPTSLEACKNLNIKPAELL 239
            P INL NFD   F +C YVLTSP SLEAC  LNI+P ELL
Sbjct:   12 PDINLYNFDDPQFQSCRYVLTSPRSLEACARLNIRPVELL 51
[Close]
BLAST of Coiled-coil domain-containing protein vs. TrEMBL
Match: A0A4V3SD13 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_009465 PE=4 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 1.841e-7
Identity = 48/150 (32.00%), Postives = 84/150 (56.00%), Query Frame = 3 
Query:  708 EKKAINVSHRRKQIEDEILIYYRELERFQNIT---------IKSAEEKFQKKLQAEQNKIQYEREYKELVNRHNRELIVEQDTNRICETMVKIIDREKQNDMFLQEKLNKMIESRCLANEAQKVREEIRKIYNLDSFDRKAVQAEIVNQV 1130
            E++      RR+++E  +  Y REL+  + IT         ++S+EE+  +K+Q  Q + Q+ RE  +++ +   E        R  E M K   +E+    FL+E+  K+ ESR LA  A+++R E++++YNLDSFD+KA    +VN++
Sbjct:  297 ERRLRQAQERRRELEKMLETYRRELQEAREITHQRARERAYLRSSEEQL-RKVQDRQQREQHIRENAKIIQQRQEEW------RRAAEAMQK--QKEEHVQKFLEEREAKIQESRNLAQAAEQLRAEMQRVYNLDSFDKKAQHVALVNEL 437

HSP 2 Score: 63.1586 bits (152), Expect = 5.788e-7
Identity = 27/40 (67.50%), Postives = 29/40 (72.50%), Query Frame = 3 
Query:  120 PQINLNNFDSDAFYNCEYVLTSPTSLEACKNLNIKPAELL 239
            P INL NFD   F +C YVLTSP SLEAC  LNI+P ELL
Sbjct:   12 PDINLYNFDDPQFQSCRYVLTSPRSLEACARLNIRPVELL 51
[Close]
BLAST of Coiled-coil domain-containing protein vs. TrEMBL
Match: A0A4S2L6P1 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_009465 PE=4 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 2.045e-7
Identity = 48/150 (32.00%), Postives = 84/150 (56.00%), Query Frame = 3 
Query:  708 EKKAINVSHRRKQIEDEILIYYRELERFQNIT---------IKSAEEKFQKKLQAEQNKIQYEREYKELVNRHNRELIVEQDTNRICETMVKIIDREKQNDMFLQEKLNKMIESRCLANEAQKVREEIRKIYNLDSFDRKAVQAEIVNQV 1130
            E++      RR+++E  +  Y REL+  + IT         ++S+EE+  +K+Q  Q + Q+ RE  +++ +   E        R  E M K   +E+    FL+E+  K+ ESR LA  A+++R E++++YNLDSFD+KA    +VN++
Sbjct:  268 ERRLRQAQERRRELEKMLETYRRELQEAREITHQRARERAYLRSSEEQL-RKVQDRQQREQHIRENAKIIQQRQEEW------RRAAEAMQK--QKEEHVQKFLEEREAKIQESRNLAQAAEQLRAEMQRVYNLDSFDKKAQHVALVNEL 408

HSP 2 Score: 63.1586 bits (152), Expect = 4.601e-7
Identity = 27/40 (67.50%), Postives = 29/40 (72.50%), Query Frame = 3 
Query:  120 PQINLNNFDSDAFYNCEYVLTSPTSLEACKNLNIKPAELL 239
            P INL NFD   F +C YVLTSP SLEAC  LNI+P ELL
Sbjct:   12 PDINLYNFDDPQFQSCRYVLTSPRSLEACARLNIRPVELL 51
[Close]
BLAST of Coiled-coil domain-containing protein vs. Ensembl Medaka
Match: ENSORLT00000012507.2 (coiled-coil domain containing 177 [Source:NCBI gene;Acc:101172777])

HSP 1 Score: 51.6026 bits (122), Expect = 1.376e-6
Identity = 24/45 (53.33%), Postives = 27/45 (60.00%), Query Frame = 3 
Query:  105 DLHQIPQINLNNFDSDAFYNCEYVLTSPTSLEACKNLNIKPAELL 239
            DL     ++L NFDS A     YVLTSP SLEAC    +KP ELL
Sbjct:   85 DLPPKMHLDLYNFDSPAAEGSRYVLTSPRSLEACARCGVKPVELL 129
[Close]
BLAST of Coiled-coil domain-containing protein vs. Planmine SMEST
Match: SMESG000005080.1 (SMESG000005080.1)

HSP 1 Score: 613.994 bits (1582), Expect = 0.000e+0
Identity = 346/350 (98.86%), Postives = 348/350 (99.43%), Query Frame = 3 
Query:   93 MSKLDLHQIPQINLNNFDSDAFYNCEYVLTSPTSLEACKNLNIKPAELLAEYDENAVYNPXXXXXXXXXXXXXXXXXXERQRLINEICSSKSQLMVEGKLLGPDKNNQAKGISDKDYHYLSQDKHERNSNNKLRRRISMQDEKKTNSFTRELSFHXXXXXXXXXXXXXXXXLCRQMKIRISRVERNRMELQMKKNREIEVKRRSEEKKAINVSHRRKQIEDEILIYYRELERFQNITIKSAEEKFQKKLQAEQNKIQYEREYKELVNRHNRELIVEQDTNRICETMVKIIDREKQNDMFLQEKLNKMIESRCLANEAQKVREEIRKIYNLDSFDRKAVQAEIVNQVMKSV 1142
            MSKLDLHQIPQINLNNFDSDAFYNCEYVLTSPTSLEACKNLNIKPAELLAEYDENAVYNPEKEKREKERKLKILRAKIERQRLINEICSSKSQLMVEGKLLGPDKNNQAKGISDKD HYLSQDKHERNS NKLRRRISMQDEKKTNSFTRELSFHEKRRELEEQAKREKEELC+QMKIR+SRVERNRMELQMKKNREIEVKRRSEEKKAINVSHRRKQIEDEILIYYRELERFQNITIKSAEEKFQKKLQAEQNKIQYEREYKELVNRHNRELIVEQDTNRICETMVKIIDREKQNDMFLQEKLNKMIESRCLANEAQKVREEIRKIYNLDSFDRKAVQAEIVNQVMKSV
Sbjct:    1 MSKLDLHQIPQINLNNFDSDAFYNCEYVLTSPTSLEACKNLNIKPAELLAEYDENAVYNPEKEKREKERKLKILRAKIERQRLINEICSSKSQLMVEGKLLGPDKNNQAKGISDKDDHYLSQDKHERNSINKLRRRISMQDEKKTNSFTRELSFHEKRRELEEQAKREKEELCQQMKIRMSRVERNRMELQMKKNREIEVKRRSEEKKAINVSHRRKQIEDEILIYYRELERFQNITIKSAEEKFQKKLQAEQNKIQYEREYKELVNRHNRELIVEQDTNRICETMVKIIDREKQNDMFLQEKLNKMIESRCLANEAQKVREEIRKIYNLDSFDRKAVQAEIVNQVMKSV 350
[Close]
BLAST of Coiled-coil domain-containing protein vs. Planmine SMEST
Match: SMESG000005080.1 (SMESG000005080.1)

HSP 1 Score: 608.986 bits (1569), Expect = 0.000e+0
Identity = 346/353 (98.02%), Postives = 348/353 (98.58%), Query Frame = 3 
Query:   93 MSKLDLHQIPQINLNNFDSDAFYNCEYVLTSPTSLEACKNLNIKPAELLAEYDENAVYNPXXXXXXXXXXXXXXXXXXERQRLINEICSSKSQLMVEGKLLGPDKNNQAKGISDKDYHYLSQDKHERNSNNKLRRRISMQ---DEKKTNSFTRELSFHXXXXXXXXXXXXXXXXLCRQMKIRISRVERNRMELQMKKNREIEVKRRSEEKKAINVSHRRKQIEDEILIYYRELERFQNITIKSAEEKFQKKLQAEQNKIQYEREYKELVNRHNRELIVEQDTNRICETMVKIIDREKQNDMFLQEKLNKMIESRCLANEAQKVREEIRKIYNLDSFDRKAVQAEIVNQVMKSV 1142
            MSKLDLHQIPQINLNNFDSDAFYNCEYVLTSPTSLEACKNLNIKPAELLAEYDENAVYNPEKEKREKERKLKILRAKIERQRLINEICSSKSQLMVEGKLLGPDKNNQAKGISDKD HYLSQDKHERNS NKLRRRISMQ   DEKKTNSFTRELSFHEKRRELEEQAKREKEELC+QMKIR+SRVERNRMELQMKKNREIEVKRRSEEKKAINVSHRRKQIEDEILIYYRELERFQNITIKSAEEKFQKKLQAEQNKIQYEREYKELVNRHNRELIVEQDTNRICETMVKIIDREKQNDMFLQEKLNKMIESRCLANEAQKVREEIRKIYNLDSFDRKAVQAEIVNQVMKSV
Sbjct:    1 MSKLDLHQIPQINLNNFDSDAFYNCEYVLTSPTSLEACKNLNIKPAELLAEYDENAVYNPEKEKREKERKLKILRAKIERQRLINEICSSKSQLMVEGKLLGPDKNNQAKGISDKDDHYLSQDKHERNSINKLRRRISMQKTKDEKKTNSFTRELSFHEKRRELEEQAKREKEELCQQMKIRMSRVERNRMELQMKKNREIEVKRRSEEKKAINVSHRRKQIEDEILIYYRELERFQNITIKSAEEKFQKKLQAEQNKIQYEREYKELVNRHNRELIVEQDTNRICETMVKIIDREKQNDMFLQEKLNKMIESRCLANEAQKVREEIRKIYNLDSFDRKAVQAEIVNQVMKSV 353
The following BLAST results are available for this feature:
BLAST of Coiled-coil domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Coiled-coil domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Coiled-coil domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Coiled-coil domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Coiled-coil domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Coiled-coil domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Coiled-coil domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Coiled-coil domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A4E0RDV34.683e-857.45Coiled-coil domain-containing protein OS=Fasciola ... [more]
A0A504YZI11.086e-757.45Uncharacterized protein OS=Fasciola gigantica OX=4... [more]
A0A4S2L7D41.182e-732.00Uncharacterized protein OS=Opisthorchis felineus O... [more]
A0A4V3SD131.841e-732.00Uncharacterized protein OS=Opisthorchis felineus O... [more]
A0A4S2L6P12.045e-732.00Uncharacterized protein OS=Opisthorchis felineus O... [more]
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BLAST of Coiled-coil domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Coiled-coil domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Coiled-coil domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Coiled-coil domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Coiled-coil domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSORLT00000012507.21.376e-653.33coiled-coil domain containing 177 [Source:NCBI gen... [more]
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BLAST of Coiled-coil domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000005080.10.000e+098.86SMESG000005080.1[more]
SMESG000005080.10.000e+098.02SMESG000005080.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30020166 ID=SMED30020166|Name=Coiled-coil domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1317bp
GTGAATATTATGAGAATTGATAATTCAATAAATCTTATTTACAAACTTAT
TTGTTTTACTGTATCAATCAATTTATTAAATGCTATAGAAACATGTCAAA
ATTAGATCTCCATCAGATTCCTCAGATCAATTTGAATAATTTCGACTCGG
ACGCTTTTTATAATTGTGAATATGTGCTAACAAGTCCAACATCTCTCGAA
GCGTGCAAGAATCTCAACATTAAACCCGCAGAATTACTGGCAGAATATGA
TGAAAACGCCGTTTACAATCCTGAGAAGGAAAAGAGAGAAAAGGAAAGAA
AATTAAAAATCTTGCGAGCAAAAATTGAGCGGCAAAGATTGATCAACGAA
ATTTGCTCTTCGAAATCTCAATTGATGGTCGAGGGTAAATTACTTGGCCC
AGATAAAAATAACCAGGCAAAGGGGATTTCAGATAAAGATTATCACTATC
TCAGTCAAGATAAGCACGAGAGGAACTCAAATAACAAACTAAGACGGCGA
ATATCAATGCAAGATGAAAAGAAAACAAATTCTTTTACGAGAGAATTGTC
TTTCCACGAGAAACGCCGAGAGCTAGAAGAACAGGCAAAACGAGAAAAAG
AAGAACTTTGCCGACAAATGAAGATACGAATATCAAGAGTTGAAAGGAAT
AGAATGGAATTGCAAATGAAAAAGAATAGAGAAATTGAAGTCAAAAGAAG
GAGTGAGGAGAAAAAAGCGATCAACGTTTCACATCGACGGAAACAAATTG
AGGATGAGATCTTGATTTACTATCGAGAATTGGAGAGATTTCAGAACATT
ACCATCAAATCTGCGGAAGAAAAATTTCAAAAGAAGTTGCAAGCCGAGCA
GAACAAAATTCAATACGAGCGAGAATACAAAGAACTCGTCAATCGCCATA
ACCGGGAATTGATAGTAGAGCAGGACACTAACAGAATCTGTGAGACAATG
GTGAAAATCATTGACAGGGAAAAACAAAATGACATGTTCTTGCAGGAGAA
ATTGAATAAAATGATTGAGTCTCGATGTTTAGCTAATGAGGCACAAAAGG
TGCGCGAGGAAATCAGGAAAATCTATAACTTAGATTCATTTGATAGGAAA
GCGGTCCAAGCAGAAATTGTTAACCAAGTCATGAAATCCGTGTGATTGTG
TTGCCTATGAGAGCTGACGGAATCGAGTCTCCAATTTATATGCATAGTGT
ATTTTTTCTGGCATTATTACTTATATGTTTTATTAATGGTAGATAACAGG
CGAAAATCTCGCCGCTTTCATTTTGAAAAATCTTTTAATTTGGTCACATT
TATGAGCTCGACCAAGT
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protein sequence of SMED30020166-orf-1

>SMED30020166-orf-1 ID=SMED30020166-orf-1|Name=SMED30020166-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=351bp
MSKLDLHQIPQINLNNFDSDAFYNCEYVLTSPTSLEACKNLNIKPAELLA
EYDENAVYNPEKEKREKERKLKILRAKIERQRLINEICSSKSQLMVEGKL
LGPDKNNQAKGISDKDYHYLSQDKHERNSNNKLRRRISMQDEKKTNSFTR
ELSFHEKRRELEEQAKREKEELCRQMKIRISRVERNRMELQMKKNREIEV
KRRSEEKKAINVSHRRKQIEDEILIYYRELERFQNITIKSAEEKFQKKLQ
AEQNKIQYEREYKELVNRHNRELIVEQDTNRICETMVKIIDREKQNDMFL
QEKLNKMIESRCLANEAQKVREEIRKIYNLDSFDRKAVQAEIVNQVMKSV
*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000020protonephridia
PLANA:0000034epidermis
PLANA:0000099neuron
PLANA:0000418head
PLANA:0002032epidermal cell
Vocabulary: INTERPRO
TermDefinition
IPR029090DUF4659
Vocabulary: molecular function
TermDefinition
GO:0000166nucleotide binding
GO:0000287magnesium ion binding
GO:0004012phospholipid-translocating ATPase activity
GO:0005524ATP binding
GO:0046872metal ion binding
Vocabulary: cellular component
TermDefinition
GO:0005802trans-Golgi network
GO:0016020membrane
GO:0016021integral component of membrane
Vocabulary: biological process
TermDefinition
GO:0015914phospholipid transport
GO:0045332phospholipid translocation
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 64..84
NoneNo IPR availableCOILSCoilCoilcoord: 241..268
NoneNo IPR availableCOILSCoilCoilcoord: 151..193
IPR029090Protein of unknown function DUF4659PFAMPF15558DUF4659coord: 55..345
e-value: 1.7E-8
score: 33.8
IPR029090Protein of unknown function DUF4659PANTHERPTHR33663FAMILY NOT NAMEDcoord: 57..344
coord: 11..86