SMED30020160

Overview
NameSMED30020160
Smed IDSMED30020160
Length (bp)2402
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30020160 (SMED30020160) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30020160 (SMED30020160) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30020160 (SMED30020160) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30020160 (SMED30020160) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30020160

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 20

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30020160SMESG000005587.1 SmedASXL_013574SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
pharynxSMED30020160SMESG000005587.1 dd_Smed_v4_8117_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30020160SMESG000005587.1 dd_Smed_v4_8117_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30020160SMESG000005587.1 dd_Smed_v4_8117_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30020160SMESG000005587.1 dd_Smed_v4_8117_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30020160SMESG000005587.1 dd_Smed_v4_8117_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30020160SMESG000005587.1 dd_Smed_v4_8117_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		whole organism asexual adult colorimetric in situ hybridization evidence
cilated neuronSMED30020160SMESG000005587.1 dd_Smed_v4_8117_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30020160SMESG000005587.1 Contig42612newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30020160SMESG000005587.1 Contig42612uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30020160SMESG000005587.1 Contig30440newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30020160SMESG000005587.1 Contig30440uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30020160SMESG000005587.1 Contig33342newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30020160SMESG000005587.1 Contig33342uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30020160SMESG000005587.1 Contig40739newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30020160SMESG000005587.1 Contig40739uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
head regionSMED30020160SMESG000005587.1 dd_Smed_v6_8117_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
reproductive organSMED30020160SMESG000005587.1 Contig33342newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30020160SMESG000005587.1 Contig33342uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
epidermisSMED30020160SMESG000005587.1 dd_Smed_v4_8117_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30020160 vs. TrEMBL
Match: R7TM76 (Uncharacterized protein (Fragment) OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_131554 PE=4 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 3.962e-12
Identity = 35/60 (58.33%), Postives = 48/60 (80.00%), Query Frame = 2 
Query: 2096 NKAFREWVKKKRQSNQMKQKEEKQKARITRLAMRQCRKSQNLAQAIHQANSFRYLDFLGY 2275
            N+AF+EW+KKK+Q  Q  +KEEKQKAR+ RL  R+CR+S  LA+AI+ + SFRY+D+ GY
Sbjct:  124 NQAFKEWLKKKKQDQQNIEKEEKQKARVHRLLARRCRRSMALARAINVSQSFRYVDYYGY 183
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BLAST of SMED30020160 vs. TrEMBL
Match: A0A267DLV5 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig003067g3 PE=4 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 5.136e-10
Identity = 38/77 (49.35%), Postives = 54/77 (70.13%), Query Frame = 2 
Query: 2045 RIQEMEQTMVIHTATERNKAFREWVKKKRQSNQMKQKEEKQKARITRLAMRQCRKSQNLAQAIHQANSFRYLDFLGY 2275
            R +E      IH+  E  KAFR W+K+K +  + ++K E+Q+ARITRLA+R+ RKSQ LA A+  ANS+RY+D+ GY
Sbjct:  388 RTEEDPPPEAIHSPEECRKAFRSWLKRKEEQRRQEEKLERQRARITRLALRRSRKSQALASALLAANSYRYMDYYGY 464
[Close]
BLAST of SMED30020160 vs. TrEMBL
Match: A0A1I8FBS7 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 5.182e-10
Identity = 38/77 (49.35%), Postives = 54/77 (70.13%), Query Frame = 2 
Query: 2045 RIQEMEQTMVIHTATERNKAFREWVKKKRQSNQMKQKEEKQKARITRLAMRQCRKSQNLAQAIHQANSFRYLDFLGY 2275
            R +E      IH+  E  KAFR W+K+K +  + ++K E+Q+ARITRLA+R+ RKSQ LA A+  ANS+RY+D+ GY
Sbjct:  338 RSEEDPPPEAIHSPEECRKAFRSWLKRKEEQRRQEEKLERQRARITRLALRRSRKSQALASALLAANSYRYMDYYGY 414
[Close]
BLAST of SMED30020160 vs. TrEMBL
Match: A0A1I8HPL5 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 73.559 bits (179), Expect = 6.552e-10
Identity = 38/77 (49.35%), Postives = 54/77 (70.13%), Query Frame = 2 
Query: 2045 RIQEMEQTMVIHTATERNKAFREWVKKKRQSNQMKQKEEKQKARITRLAMRQCRKSQNLAQAIHQANSFRYLDFLGY 2275
            R +E      IH+  E  KAFR W+K+K +  + ++K E+Q+ARITRLA+R+ RKSQ LA A+  ANS+RY+D+ GY
Sbjct:  337 RSEEDPPPEAIHSPEECRKAFRSWLKRKEEQRRQEEKLERQRARITRLALRRSRKSQALASALLAANSYRYMDYYGY 413
[Close]
BLAST of SMED30020160 vs. TrEMBL
Match: A0A267FV66 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig009650g2 PE=4 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 8.256e-10
Identity = 31/66 (46.97%), Postives = 51/66 (77.27%), Query Frame = 2 
Query: 2078 HTATERNKAFREWVKKKRQSNQMKQKEEKQKARITRLAMRQCRKSQNLAQAIHQANSFRYLDFLGY 2275
            H+  +  +AF++W+++K +  + ++K E+Q+ARITRLA+R+ RKSQ LA A+  A+S+RYLD+ GY
Sbjct:  340 HSQEDCRRAFKQWLRRKEEQRRQEEKLERQRARITRLALRRSRKSQALANALMAADSYRYLDYHGY 405
[Close]
BLAST of SMED30020160 vs. Planmine SMEST
Match: SMESG000005587.1 (SMESG000005587.1)

HSP 1 Score: 1307.35 bits (3382), Expect = 0.000e+0
Identity = 712/719 (99.03%), Postives = 714/719 (99.30%), Query Frame = 2 
Query:  122 MDQVDFQDSNSQSRPKSPLKLVHSDINNVEKAETPTESLNEIIMSHSKPFEKDNGENFNEKHFSTTINNRNGKISPDEMVKNPNENSFRRGIDSEDNGEIQWEIETVKDHSNSIEKRKSDESSIEIVKGKYDIKKEILETQHRDYGLAEPKPNSNIDFSRQSSENPSPILSGSNFSDYNLKSGNPNLDDQYMSNRFFIDDTNESDHIKYSDNNINLFELTPDQQLAIRELELRKPQSDFEEDYDYSMNYDNIPDYDVKEKLESLNAQLANEPSPPEPEHRNSRVAFKEELVSLVAPVQEYPLSESEQEEYNDTPEYLRYHETRNENVIEIESPVSLSRENYENAQPNLEKKDNENSNAAFESYNYERNDNKSEQDPEPISQPNVQSDFLVKSQPEKTYETKFIEKNELNLETNLHSENEINLENKKTVKELEANSNKEKHNDYNSDQEIDIVVSRGGKFQLMDQSDMVDSSGNIVNPDEEILEPTNKQQSRPISAPATKGNRNEIWSSMHTGSPNNSVRSEMNYQSKTTTRPNSNVPKVDXXXXXXXXXXXXXXXXXXXXXDEIELKNKREMNEKCFNAWLQNKNKVLXXXXXXXXXXXXXXXXXXXXXXXXNKAQEIETLKELARNXXXXXXXXXXXXXYRIQEMEQTMVIHTATERNKAFREWVKKKRQSNQMKQKEEKQKARITRLAMRQCRKSQNLAQAIHQANSFRYLDFLGYP 2278
            MDQVDFQD NSQ RPKSPLKLVHSDINNVEKAETPTESLNEIIMS SKPFEKDNGENFNEKHFSTTINNRNGKISPDEMVKNPNENSFRRGIDSEDNGEIQWEIETVKDHSNSIEKRKSDESSIEIVKGKYDIKKEILETQHRDYGL+EPKPNS+IDFS QSSENPSPILSGSNFSDYNLKSGNPNLDDQYMSNRFFIDDTNESDHIKYSDNNINLFELTPDQQLAIRELELRKPQSDFEEDYDYSMNYDNIPDYDVKEKLESLNAQLANEPSPPEPEHRNSRVAFKEELVSLVAPVQEYPLSESEQEEYNDTPEYLRYHETRNENVIEIESPVSLSRENYENAQPNLEKKDNENSNAAF SYNYERNDNKSEQDPEPISQPNVQSDFLVKSQPEKTYETKFIEKNELNLETNLHSENEINLENKKTVKELEANSNKEKHNDYNSDQEIDIVVSRGGKFQLMDQSDMVDSSGNIVNPDEEILEPTNKQQSRPISAPATKGNRNEIWSSMHTGSPNNSVRSEMNYQSKTTTRPNSNVPKVDRELLQRQKEKEEKRKKEQQERDEIELKNKREMNEKCFNAWLQNKNKVLKEETERKKKEDEEERKQQEKTREENKAQEIETLKELARNKELQRRREEKLRQYRIQEMEQTMVIHTATERNKAFREWVKKKRQSNQMKQKEEKQKARITRLAMRQCRKSQNLAQAIHQANSFRYLDFLGYP
Sbjct:    1 MDQVDFQDRNSQGRPKSPLKLVHSDINNVEKAETPTESLNEIIMSPSKPFEKDNGENFNEKHFSTTINNRNGKISPDEMVKNPNENSFRRGIDSEDNGEIQWEIETVKDHSNSIEKRKSDESSIEIVKGKYDIKKEILETQHRDYGLSEPKPNSDIDFSGQSSENPSPILSGSNFSDYNLKSGNPNLDDQYMSNRFFIDDTNESDHIKYSDNNINLFELTPDQQLAIRELELRKPQSDFEEDYDYSMNYDNIPDYDVKEKLESLNAQLANEPSPPEPEHRNSRVAFKEELVSLVAPVQEYPLSESEQEEYNDTPEYLRYHETRNENVIEIESPVSLSRENYENAQPNLEKKDNENSNAAFISYNYERNDNKSEQDPEPISQPNVQSDFLVKSQPEKTYETKFIEKNELNLETNLHSENEINLENKKTVKELEANSNKEKHNDYNSDQEIDIVVSRGGKFQLMDQSDMVDSSGNIVNPDEEILEPTNKQQSRPISAPATKGNRNEIWSSMHTGSPNNSVRSEMNYQSKTTTRPNSNVPKVDRELLQRQKEKEEKRKKEQQERDEIELKNKREMNEKCFNAWLQNKNKVLKEETERKKKEDEEERKQQEKTREENKAQEIETLKELARNKELQRRREEKLRQYRIQEMEQTMVIHTATERNKAFREWVKKKRQSNQMKQKEEKQKARITRLAMRQCRKSQNLAQAIHQANSFRYLDFLGYP 719
[Close]
BLAST of SMED30020160 vs. Planmine SMEST
Match: SMESG000005587.1 (SMESG000005587.1)

HSP 1 Score: 617.461 bits (1591), Expect = 0.000e+0
Identity = 369/370 (99.73%), Postives = 369/370 (99.73%), Query Frame = 2 
Query: 1169 KKDNENSNAAFESYNYERNDNKSEQDPEPISQPNVQSDFLVKSQPEKTYETKFIEKNELNLETNLHSENEINLENKKTVKELEANSNKEKHNDYNSDQEIDIVVSRGGKFQLMDQSDMVDSSGNIVNPDEEILEPTNKQQSRPISAPATKGNRNEIWSSMHTGSPNNSVRSEMNYQSKTTTRPNSNVPKVDXXXXXXXXXXXXXXXXXXXXXDEIELKNKREMNEKCFNAWLQNKNKVLXXXXXXXXXXXXXXXXXXXXXXXXNKAQEIETLKELARNXXXXXXXXXXXXXYRIQEMEQTMVIHTATERNKAFREWVKKKRQSNQMKQKEEKQKARITRLAMRQCRKSQNLAQAIHQANSFRYLDFLGYP 2278
            KKDNENSNAAF SYNYERNDNKSEQDPEPISQPNVQSDFLVKSQPEKTYETKFIEKNELNLETNLHSENEINLENKKTVKELEANSNKEKHNDYNSDQEIDIVVSRGGKFQLMDQSDMVDSSGNIVNPDEEILEPTNKQQSRPISAPATKGNRNEIWSSMHTGSPNNSVRSEMNYQSKTTTRPNSNVPKVDRELLQRQKEKEEKRKKEQQERDEIELKNKREMNEKCFNAWLQNKNKVLKEETERKKKEDEEERKQQEKTREENKAQEIETLKELARNKELQRRREEKLRQYRIQEMEQTMVIHTATERNKAFREWVKKKRQSNQMKQKEEKQKARITRLAMRQCRKSQNLAQAIHQANSFRYLDFLGYP
Sbjct:   39 KKDNENSNAAFISYNYERNDNKSEQDPEPISQPNVQSDFLVKSQPEKTYETKFIEKNELNLETNLHSENEINLENKKTVKELEANSNKEKHNDYNSDQEIDIVVSRGGKFQLMDQSDMVDSSGNIVNPDEEILEPTNKQQSRPISAPATKGNRNEIWSSMHTGSPNNSVRSEMNYQSKTTTRPNSNVPKVDRELLQRQKEKEEKRKKEQQERDEIELKNKREMNEKCFNAWLQNKNKVLKEETERKKKEDEEERKQQEKTREENKAQEIETLKELARNKELQRRREEKLRQYRIQEMEQTMVIHTATERNKAFREWVKKKRQSNQMKQKEEKQKARITRLAMRQCRKSQNLAQAIHQANSFRYLDFLGYP 408
[Close]
BLAST of SMED30020160 vs. Planmine SMEST
Match: SMESG000005587.1 (SMESG000005587.1)

HSP 1 Score: 613.224 bits (1580), Expect = 0.000e+0
Identity = 369/370 (99.73%), Postives = 369/370 (99.73%), Query Frame = 2 
Query: 1169 KKDNENSNAAFESYNYERNDNKSEQDPEPISQPNVQSDFLVKSQPEKTYETKFIEKNELNLETNLHSENEINLENKKTVKELEANSNKEKHNDYNSDQEIDIVVSRGGKFQLMDQSDMVDSSGNIVNPDEEILEPTNKQQSRPISAPATKGNRNEIWSSMHTGSPNNSVRSEMNYQSKTTTRPNSNVPKVDXXXXXXXXXXXXXXXXXXXXXDEIELKNKREMNEKCFNAWLQNKNKVLXXXXXXXXXXXXXXXXXXXXXXXXNKAQEIETLKELARNXXXXXXXXXXXXXYRIQEMEQTMVIHTATERNKAFREWVKKKRQSNQMKQKEEKQKARITRLAMRQCRKSQNLAQAIHQANSFRYLDFLGYP 2278
            KKDNENSNAAF SYNYERNDNKSEQDPEPISQPNVQSDFLVKSQPEKTYETKFIEKNELNLETNLHSENEINLENKKTVKELEANSNKEKHNDYNSDQEIDIVVSRGGKFQLMDQSDMVDSSGNIVNPDEEILEPTNKQQSRPISAPATKGNRNEIWSSMHTGSPNNSVRSEMNYQSKTTTRPNSNVPKVDRELLQRQKEKEEKRKKEQQERDEIELKNKREMNEKCFNAWLQNKNKVLKEETERKKKEDEEERKQQEKTREENKAQEIETLKELARNKELQRRREEKLRQYRIQEMEQTMVIHTATERNKAFREWVKKKRQSNQMKQKEEKQKARITRLAMRQCRKSQNLAQAIHQANSFRYLDFLGYP
Sbjct:   58 KKDNENSNAAFISYNYERNDNKSEQDPEPISQPNVQSDFLVKSQPEKTYETKFIEKNELNLETNLHSENEINLENKKTVKELEANSNKEKHNDYNSDQEIDIVVSRGGKFQLMDQSDMVDSSGNIVNPDEEILEPTNKQQSRPISAPATKGNRNEIWSSMHTGSPNNSVRSEMNYQSKTTTRPNSNVPKVDRELLQRQKEKEEKRKKEQQERDEIELKNKREMNEKCFNAWLQNKNKVLKEETERKKKEDEEERKQQEKTREENKAQEIETLKELARNKELQRRREEKLRQYRIQEMEQTMVIHTATERNKAFREWVKKKRQSNQMKQKEEKQKARITRLAMRQCRKSQNLAQAIHQANSFRYLDFLGYP 427
[Close]
BLAST of SMED30020160 vs. Planmine SMEST
Match: SMESG000005587.1 (SMESG000005587.1)

HSP 1 Score: 605.905 bits (1561), Expect = 0.000e+0
Identity = 368/372 (98.92%), Postives = 369/372 (99.19%), Query Frame = 2 
Query: 1169 KKDNENSNAAFESYNYERNDNKSEQDPEPISQPNVQSDFLVKSQPEKTYETKFIEKNELNLETNLHSENEINLENKKTVKELEANSNKEKHNDYNSDQEIDIVVSRGGKFQLMDQSDMVDSSGNIVNPDEEILEPTNKQQSRPISAPATKGNRNEIWSSMHTGSPNNSVRSEMNYQSKTTTRPNS--NVPKVDXXXXXXXXXXXXXXXXXXXXXDEIELKNKREMNEKCFNAWLQNKNKVLXXXXXXXXXXXXXXXXXXXXXXXXNKAQEIETLKELARNXXXXXXXXXXXXXYRIQEMEQTMVIHTATERNKAFREWVKKKRQSNQMKQKEEKQKARITRLAMRQCRKSQNLAQAIHQANSFRYLDFLGYP 2278
            KKDNENSNAAF SYNYERNDNKSEQDPEPISQPNVQSDFLVKSQPEKTYETKFIEKNELNLETNLHSENEINLENKKTVKELEANSNKEKHNDYNSDQEIDIVVSRGGKFQLMDQSDMVDSSGNIVNPDEEILEPTNKQQSRPISAPATKGNRNEIWSSMHTGSPNNSVRSEMNYQSKTTTRPNS  +VPKVDRELLQRQKEKEEKRKKEQQERDEIELKNKREMNEKCFNAWLQNKNKVLKEETERKKKEDEEERKQQEKTREENKAQEIETLKELARNKELQRRREEKLRQYRIQEMEQTMVIHTATERNKAFREWVKKKRQSNQMKQKEEKQKARITRLAMRQCRKSQNLAQAIHQANSFRYLDFLGYP
Sbjct:   58 KKDNENSNAAFISYNYERNDNKSEQDPEPISQPNVQSDFLVKSQPEKTYETKFIEKNELNLETNLHSENEINLENKKTVKELEANSNKEKHNDYNSDQEIDIVVSRGGKFQLMDQSDMVDSSGNIVNPDEEILEPTNKQQSRPISAPATKGNRNEIWSSMHTGSPNNSVRSEMNYQSKTTTRPNSKYDVPKVDRELLQRQKEKEEKRKKEQQERDEIELKNKREMNEKCFNAWLQNKNKVLKEETERKKKEDEEERKQQEKTREENKAQEIETLKELARNKELQRRREEKLRQYRIQEMEQTMVIHTATERNKAFREWVKKKRQSNQMKQKEEKQKARITRLAMRQCRKSQNLAQAIHQANSFRYLDFLGYP 429
The following BLAST results are available for this feature:
BLAST of SMED30020160 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020160 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020160 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020160 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020160 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020160 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020160 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020160 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
R7TM763.962e-1258.33Uncharacterized protein (Fragment) OS=Capitella te... [more]
A0A267DLV55.136e-1049.35Uncharacterized protein (Fragment) OS=Macrostomum ... [more]
A0A1I8FBS75.182e-1049.35Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8HPL56.552e-1049.35Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267FV668.256e-1046.97Uncharacterized protein (Fragment) OS=Macrostomum ... [more]
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BLAST of SMED30020160 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020160 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020160 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020160 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020160 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020160 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4
Match NameE-valueIdentityDescription
SMESG000005587.10.000e+099.03SMESG000005587.1[more]
SMESG000005587.10.000e+099.73SMESG000005587.1[more]
SMESG000005587.10.000e+099.73SMESG000005587.1[more]
SMESG000005587.10.000e+098.92SMESG000005587.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30020160 ID=SMED30020160|Name=SMED30020160|organism=Schmidtea mediterranea sexual|type=transcript|length=2402bp
ATATATTTTTAGCTAATTTTAAGCAATTTCATAGCTTTAATAAATTTTAA
ATTATTCATCATTTAATTTTAAAATTTAATTGATTCTGCATAAAGAAGCT
TGGAAAATTTTTAATAATTCGATGGATCAAGTTGATTTTCAAGATAGCAA
TTCCCAAAGTCGTCCAAAATCTCCATTGAAATTGGTTCACAGCGATATCA
ATAATGTGGAAAAAGCAGAAACTCCAACCGAAAGTCTCAATGAAATAATT
ATGTCTCATTCGAAACCATTTGAAAAAGATAATGGGGAAAATTTTAATGA
AAAACATTTTTCAACAACAATTAATAATAGAAATGGAAAAATAAGTCCGG
ATGAAATGGTGAAAAATCCAAATGAAAATAGTTTTAGAAGAGGAATTGAC
AGTGAAGACAATGGGGAAATTCAGTGGGAAATTGAAACAGTAAAAGATCA
CTCAAACTCCATAGAGAAACGGAAGAGTGATGAATCAAGTATAGAAATTG
TAAAGGGAAAATATGATATAAAGAAAGAAATACTAGAAACCCAACACAGA
GATTATGGACTTGCTGAACCAAAACCAAATTCAAACATAGATTTTTCGAG
GCAATCATCCGAAAATCCTAGTCCCATTTTATCTGGTTCAAATTTCTCAG
ATTATAATTTGAAATCTGGAAATCCAAATCTTGATGATCAGTATATGAGC
AATAGATTTTTCATAGACGATACAAATGAGTCTGATCATATTAAATACTC
TGATAACAACATCAATCTCTTTGAATTGACCCCCGATCAACAGTTGGCTA
TACGTGAACTGGAATTGCGAAAACCACAGTCAGACTTTGAAGAAGATTAT
GATTATTCAATGAATTACGACAACATACCAGATTATGATGTAAAAGAGAA
ACTTGAATCATTAAATGCACAATTGGCAAATGAACCATCCCCACCAGAGC
CGGAACATCGCAATAGTCGGGTAGCATTTAAAGAGGAACTGGTTAGTTTG
GTTGCCCCTGTTCAAGAATATCCTTTGTCGGAAAGTGAACAAGAAGAATA
TAACGATACACCTGAATACCTCAGATATCATGAAACCCGAAATGAAAATG
TCATAGAAATAGAATCACCTGTTTCTTTGTCGCGGGAAAATTATGAAAAT
GCACAACCAAATCTAGAAAAAAAAGACAATGAAAATTCCAATGCTGCTTT
TGAATCTTACAACTATGAAAGAAATGATAATAAATCAGAACAAGATCCAG
AACCAATATCACAACCAAATGTTCAGTCTGATTTCTTAGTGAAATCACAA
CCTGAAAAAACCTATGAAACAAAATTTATAGAGAAAAATGAATTAAATTT
GGAAACAAATCTACATTCGGAAAATGAGATAAATTTGGAAAACAAAAAGA
CAGTAAAAGAACTTGAAGCAAATTCAAATAAAGAAAAACACAATGATTAC
AACAGCGATCAAGAAATTGACATTGTTGTAAGTAGAGGTGGAAAGTTTCA
GTTAATGGACCAAAGTGATATGGTCGATTCATCAGGAAATATAGTTAATC
CCGATGAAGAAATCCTCGAGCCAACCAATAAGCAACAGTCGAGACCTATT
TCAGCTCCAGCCACGAAAGGAAACCGTAATGAGATTTGGTCAAGTATGCA
TACAGGATCGCCAAATAATTCAGTGAGATCAGAAATGAATTATCAGTCAA
AAACCACTACCAGACCAAATTCCAATGTTCCAAAAGTTGACAGAGAGCTT
TTACAGCGCCAAAAAGAAAAAGAAGAAAAAAGAAAAAAAGAACAACAAGA
ACGAGATGAAATCGAATTGAAGAATAAAAGAGAAATGAATGAAAAATGTT
TTAATGCCTGGCTTCAAAACAAAAACAAAGTTTTAAAAGAAGAAACAGAG
CGCAAAAAGAAAGAGGATGAGGAAGAGAGAAAACAACAAGAAAAAACGAG
AGAAGAGAATAAAGCACAAGAAATAGAAACATTGAAAGAATTAGCGCGAA
ACAAAGAACTTCAAAGACGCAGAGAAGAGAAATTGCGACAATATCGAATC
CAAGAGATGGAACAAACCATGGTAATTCATACGGCAACTGAGCGCAATAA
AGCTTTTAGAGAGTGGGTCAAAAAGAAGAGACAGAGTAACCAAATGAAGC
AGAAAGAAGAAAAACAGAAAGCTCGCATTACCAGACTGGCTATGAGACAG
TGCAGAAAATCCCAAAATCTTGCTCAGGCAATACATCAAGCCAACTCATT
TCGATACCTTGATTTCCTCGGCTACCCATGATAAGCACCTTTGCCACAAA
ATTATGAATGATTATTTGAATCACAGTTAATTTTGCACTTGTATATCAGC
TGCATATTTCCGTGGCCATGATTTTGGCATCGTTCTATAATGATGATACT
AA
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protein sequence of SMED30020160-orf-1

>SMED30020160-orf-1 ID=SMED30020160-orf-1|Name=SMED30020160-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=720bp
MDQVDFQDSNSQSRPKSPLKLVHSDINNVEKAETPTESLNEIIMSHSKPF
EKDNGENFNEKHFSTTINNRNGKISPDEMVKNPNENSFRRGIDSEDNGEI
QWEIETVKDHSNSIEKRKSDESSIEIVKGKYDIKKEILETQHRDYGLAEP
KPNSNIDFSRQSSENPSPILSGSNFSDYNLKSGNPNLDDQYMSNRFFIDD
TNESDHIKYSDNNINLFELTPDQQLAIRELELRKPQSDFEEDYDYSMNYD
NIPDYDVKEKLESLNAQLANEPSPPEPEHRNSRVAFKEELVSLVAPVQEY
PLSESEQEEYNDTPEYLRYHETRNENVIEIESPVSLSRENYENAQPNLEK
KDNENSNAAFESYNYERNDNKSEQDPEPISQPNVQSDFLVKSQPEKTYET
KFIEKNELNLETNLHSENEINLENKKTVKELEANSNKEKHNDYNSDQEID
IVVSRGGKFQLMDQSDMVDSSGNIVNPDEEILEPTNKQQSRPISAPATKG
NRNEIWSSMHTGSPNNSVRSEMNYQSKTTTRPNSNVPKVDRELLQRQKEK
EEKRKKEQQERDEIELKNKREMNEKCFNAWLQNKNKVLKEETERKKKEDE
EERKQQEKTREENKAQEIETLKELARNKELQRRREEKLRQYRIQEMEQTM
VIHTATERNKAFREWVKKKRQSNQMKQKEEKQKARITRLAMRQCRKSQNL
AQAIHQANSFRYLDFLGYP*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000020protonephridia
PLANA:0000034epidermis
PLANA:0000099neuron
PLANA:0000418head
PLANA:0002032epidermal cell
PLANA:0002089reproductive organ
Vocabulary: INTERPRO
TermDefinition
IPR026687CCDC181
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 539..574
NoneNo IPR availableCOILSCoilCoilcoord: 578..647
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 64..83
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..39
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 541..566
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 142..164
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 590..619
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 480..566
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 482..536
IPR026687Coiled-coil domain-containing protein 181PANTHERPTHR14320FAMILY NOT NAMEDcoord: 222..534
coord: 481..714