SMED30020153

Overview
NameSMED30020153
Smed IDSMED30020153
Length (bp)1375
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30020153 (SMED30020153) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30020153 (SMED30020153) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30020153 (SMED30020153) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30020153 (SMED30020153) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30020153

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 12

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30020153h1SMcG0005696 Contig4720uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30020153h1SMcG0005696 Contig4720newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
gutSMED30020153h1SMcG0005696 Contig1559GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
epidermisSMED30020153h1SMcG0005696 Contig1559GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30020153h1SMcG0005696 Contig1559GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
central nervous systemSMED30020153h1SMcG0005696 Contig1559GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30020153h1SMcG0010767 Contig4720uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30020153h1SMcG0010767 Contig4720newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
gutSMED30020153h1SMcG0005696 dd_Smed_v4_5510_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30020153h1SMcG0005696 dd_Smed_v4_5510_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30020153h1SMcG0005696 Contig29962newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30020153h1SMcG0005696 Contig29962uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30020153 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.004023:23841..25352 -6796
Homology
[Close]
BLAST of SMED30020153 vs. Ensembl Human
Match: TRAF3 (TNF receptor associated factor 3 [Source:HGNC Symbol;Acc:HGNC:12033])

HSP 1 Score: 50.447 bits (119), Expect = 4.691e-6
Identity = 62/267 (23.22%), Postives = 117/267 (43.82%), Query Frame = 2 
Query:  401 ANNQQIPI-EKSSSLQFIN------NFTSTKPGTLSEITVETASSKMELNDE--------ESLKDLLYKNAAKI----KLMDEEIRMLKSVNESLIHMMDHWRTMLDNKLESMNSEMRTTFQDIKLEIEGAAKRKQSQSDESDILSTSISK---------TKLTEPRISLE----RKIAGIYTWNIDRVMEKR-----GKRGSIWSEFFYTRNNHYKMRLQFHPNG-FVCTGTGLSIYLFLYPSETNSKGLWPFKADVTMKIVNKAN 1087
              NQQI   E SS++Q +N      N    K   L   +VE   S   L+++        E  K++L  N +KI    +++D +   LK +++ +     +W      + +SM S + +  Q+   E+E   K     +  + +L + +S+          +L +  +  +        G+  W I     ++     GK  S++S+ FYT    YKM  + + NG  +  GT LS++  +   E ++   WPFK  VT+ ++++ +
Sbjct:  243 GTNQQIKAHEASSAVQHVNLLKEWSNSLEKKVSLLQNESVEKNKSIQSLHNQICSFEIEIERQKEMLRNNESKILHLQRVIDSQAEKLKELDKEIRPFRQNW-----EEADSMKSSVES-LQNRVTELESVDKSAGQVARNTGLLESQLSRHDQMLSVHDIRLADMDLRFQVLETASYNGVLIWKIRDYKRRKQEAVMGKTLSLYSQPFYTGYFGYKMCARVYLNGDGMGKGTHLSLFFVIMRGEYDALLPWPFKQKVTLMLMDQGS 503
[Close]
BLAST of SMED30020153 vs. Ensembl Human
Match: TRAF3 (TNF receptor associated factor 3 [Source:HGNC Symbol;Acc:HGNC:12033])

HSP 1 Score: 50.447 bits (119), Expect = 4.691e-6
Identity = 62/267 (23.22%), Postives = 117/267 (43.82%), Query Frame = 2 
Query:  401 ANNQQIPI-EKSSSLQFIN------NFTSTKPGTLSEITVETASSKMELNDE--------ESLKDLLYKNAAKI----KLMDEEIRMLKSVNESLIHMMDHWRTMLDNKLESMNSEMRTTFQDIKLEIEGAAKRKQSQSDESDILSTSISK---------TKLTEPRISLE----RKIAGIYTWNIDRVMEKR-----GKRGSIWSEFFYTRNNHYKMRLQFHPNG-FVCTGTGLSIYLFLYPSETNSKGLWPFKADVTMKIVNKAN 1087
              NQQI   E SS++Q +N      N    K   L   +VE   S   L+++        E  K++L  N +KI    +++D +   LK +++ +     +W      + +SM S + +  Q+   E+E   K     +  + +L + +S+          +L +  +  +        G+  W I     ++     GK  S++S+ FYT    YKM  + + NG  +  GT LS++  +   E ++   WPFK  VT+ ++++ +
Sbjct:  243 GTNQQIKAHEASSAVQHVNLLKEWSNSLEKKVSLLQNESVEKNKSIQSLHNQICSFEIEIERQKEMLRNNESKILHLQRVIDSQAEKLKELDKEIRPFRQNW-----EEADSMKSSVES-LQNRVTELESVDKSAGQVARNTGLLESQLSRHDQMLSVHDIRLADMDLRFQVLETASYNGVLIWKIRDYKRRKQEAVMGKTLSLYSQPFYTGYFGYKMCARVYLNGDGMGKGTHLSLFFVIMRGEYDALLPWPFKQKVTLMLMDQGS 503
[Close]
BLAST of SMED30020153 vs. Ensembl Human
Match: TRAF3 (TNF receptor associated factor 3 [Source:HGNC Symbol;Acc:HGNC:12033])

HSP 1 Score: 50.447 bits (119), Expect = 4.918e-6
Identity = 62/267 (23.22%), Postives = 117/267 (43.82%), Query Frame = 2 
Query:  401 ANNQQIPI-EKSSSLQFIN------NFTSTKPGTLSEITVETASSKMELNDE--------ESLKDLLYKNAAKI----KLMDEEIRMLKSVNESLIHMMDHWRTMLDNKLESMNSEMRTTFQDIKLEIEGAAKRKQSQSDESDILSTSISK---------TKLTEPRISLE----RKIAGIYTWNIDRVMEKR-----GKRGSIWSEFFYTRNNHYKMRLQFHPNG-FVCTGTGLSIYLFLYPSETNSKGLWPFKADVTMKIVNKAN 1087
              NQQI   E SS++Q +N      N    K   L   +VE   S   L+++        E  K++L  N +KI    +++D +   LK +++ +     +W      + +SM S + +  Q+   E+E   K     +  + +L + +S+          +L +  +  +        G+  W I     ++     GK  S++S+ FYT    YKM  + + NG  +  GT LS++  +   E ++   WPFK  VT+ ++++ +
Sbjct:  218 GTNQQIKAHEASSAVQHVNLLKEWSNSLEKKVSLLQNESVEKNKSIQSLHNQICSFEIEIERQKEMLRNNESKILHLQRVIDSQAEKLKELDKEIRPFRQNW-----EEADSMKSSVES-LQNRVTELESVDKSAGQVARNTGLLESQLSRHDQMLSVHDIRLADMDLRFQVLETASYNGVLIWKIRDYKRRKQEAVMGKTLSLYSQPFYTGYFGYKMCARVYLNGDGMGKGTHLSLFFVIMRGEYDALLPWPFKQKVTLMLMDQGS 478
[Close]
BLAST of SMED30020153 vs. Ensembl Human
Match: TRAF3 (TNF receptor associated factor 3 [Source:HGNC Symbol;Acc:HGNC:12033])

HSP 1 Score: 50.447 bits (119), Expect = 4.918e-6
Identity = 62/267 (23.22%), Postives = 117/267 (43.82%), Query Frame = 2 
Query:  401 ANNQQIPI-EKSSSLQFIN------NFTSTKPGTLSEITVETASSKMELNDE--------ESLKDLLYKNAAKI----KLMDEEIRMLKSVNESLIHMMDHWRTMLDNKLESMNSEMRTTFQDIKLEIEGAAKRKQSQSDESDILSTSISK---------TKLTEPRISLE----RKIAGIYTWNIDRVMEKR-----GKRGSIWSEFFYTRNNHYKMRLQFHPNG-FVCTGTGLSIYLFLYPSETNSKGLWPFKADVTMKIVNKAN 1087
              NQQI   E SS++Q +N      N    K   L   +VE   S   L+++        E  K++L  N +KI    +++D +   LK +++ +     +W      + +SM S + +  Q+   E+E   K     +  + +L + +S+          +L +  +  +        G+  W I     ++     GK  S++S+ FYT    YKM  + + NG  +  GT LS++  +   E ++   WPFK  VT+ ++++ +
Sbjct:  218 GTNQQIKAHEASSAVQHVNLLKEWSNSLEKKVSLLQNESVEKNKSIQSLHNQICSFEIEIERQKEMLRNNESKILHLQRVIDSQAEKLKELDKEIRPFRQNW-----EEADSMKSSVES-LQNRVTELESVDKSAGQVARNTGLLESQLSRHDQMLSVHDIRLADMDLRFQVLETASYNGVLIWKIRDYKRRKQEAVMGKTLSLYSQPFYTGYFGYKMCARVYLNGDGMGKGTHLSLFFVIMRGEYDALLPWPFKQKVTLMLMDQGS 478
[Close]
BLAST of SMED30020153 vs. Ensembl Xenopus
Match: ssh2 (slingshot protein phosphatase 2 [Source:Xenbase;Acc:XB-GENE-993093])

HSP 1 Score: 49.6766 bits (117), Expect = 9.497e-6
Identity = 55/239 (23.01%), Postives = 112/239 (46.86%), Query Frame = 2 
Query:  425 EKSSSLQFINNFTSTKPGTLSEITVETASSKMELNDEES----LKDLLYKNAAKIKLMDEEIRMLKSVNESLIHMMDHWRTMLDNKLESMNSEMRTTFQDIKLEIEGAAKRKQSQSDESDILSTSISKTKLTEPRISLERKIA--GIYTWNIDRVMEKR-----GKRGSIWSEFFYTRNNHYKMRLQFHPNG-FVCTGTGLSIYLFLYPSETNSKGLWPFKADVTMKIVNKANPSKSKI 1105
            EK+ S+Q ++N        +    +E    K +L + E+    L+ ++   A K+K +D+EIR L+   +    M  ++ + L N+L    S++    ++      G  + + ++ D+  +LS    +    + R  +    +  G+  W I     ++     GK  S++S+ FYT    YKM  + + NG  +  GT LS++  +   E ++   WPFK  V++ ++++  PS+  +
Sbjct:  278 EKNKSIQILHN-------QMCSFEIEIERHKEQLRNNEARIIQLQRVIEGQADKLKDLDKEIRNLRQTGDEADGMKSNYES-LQNRL----SQLEVMAKNAASWNPGVLETQINRHDQ--MLSVHDIRLADMDLRFQVLETASYNGVLIWKIRDYKRRKQEAVMGKTLSLYSQPFYTGYFGYKMCARVYLNGDGMGKGTHLSLFFVIMRGEYDALLPWPFKQKVSLMLMDQG-PSRRHL 501
[Close]
BLAST of SMED30020153 vs. Ensembl Xenopus
Match: ssh2 (slingshot protein phosphatase 2 [Source:Xenbase;Acc:XB-GENE-993093])

HSP 1 Score: 49.2914 bits (116), Expect = 9.618e-6
Identity = 55/239 (23.01%), Postives = 112/239 (46.86%), Query Frame = 2 
Query:  425 EKSSSLQFINNFTSTKPGTLSEITVETASSKMELNDEES----LKDLLYKNAAKIKLMDEEIRMLKSVNESLIHMMDHWRTMLDNKLESMNSEMRTTFQDIKLEIEGAAKRKQSQSDESDILSTSISKTKLTEPRISLERKIA--GIYTWNIDRVMEKR-----GKRGSIWSEFFYTRNNHYKMRLQFHPNG-FVCTGTGLSIYLFLYPSETNSKGLWPFKADVTMKIVNKANPSKSKI 1105
            EK+ S+Q ++N        +    +E    K +L + E+    L+ ++   A K+K +D+EIR L+   +    M  ++ + L N+L    S++    ++      G  + + ++ D+  +LS    +    + R  +    +  G+  W I     ++     GK  S++S+ FYT    YKM  + + NG  +  GT LS++  +   E ++   WPFK  V++ ++++  PS+  +
Sbjct:  253 EKNKSIQILHN-------QMCSFEIEIERHKEQLRNNEARIIQLQRVIEGQADKLKDLDKEIRNLRQTGDEADGMKSNYES-LQNRL----SQLEVMAKNAASWNPGVLETQINRHDQ--MLSVHDIRLADMDLRFQVLETASYNGVLIWKIRDYKRRKQEAVMGKTLSLYSQPFYTGYFGYKMCARVYLNGDGMGKGTHLSLFFVIMRGEYDALLPWPFKQKVSLMLMDQG-PSRRHL 476
[Close]
BLAST of SMED30020153 vs. Ensembl Mouse
Match: Traf3 (TNF receptor-associated factor 3 [Source:MGI Symbol;Acc:MGI:108041])

HSP 1 Score: 51.2174 bits (121), Expect = 2.011e-6
Identity = 62/267 (23.22%), Postives = 117/267 (43.82%), Query Frame = 2 
Query:  401 ANNQQIPI-EKSSSLQFIN------NFTSTKPGTLSEITVETASSKMELNDE--------ESLKDLLYKNAAKI----KLMDEEIRMLKSVNESLIHMMDHWRTMLDNKLESMNSEMRTTFQDIKLEIEGAAKRKQSQSDESDILSTSISK---------TKLTEPRISLE----RKIAGIYTWNIDRVMEKR-----GKRGSIWSEFFYTRNNHYKMRLQFHPNG-FVCTGTGLSIYLFLYPSETNSKGLWPFKADVTMKIVNKAN 1087
              NQQI   E SS++Q +N      N    K   L   +VE   S   L+++        E  K++L  N +KI    +++D +   LK +++ +     +W      + +SM S + +  Q+   E+E   K     +  + +L + +S+          +L +  +  +        G+  W I     ++     GK  S++S+ FYT    YKM  + + NG  +  GT LS++  +   E ++   WPFK  VT+ ++++ +
Sbjct:  242 GTNQQIKAHEASSAVQHVNLLKEWSNSLEKKVSLLQNESVEKNKSIQSLHNQICSFEIEIERQKEMLRNNESKILHLQRVIDSQAEKLKELDKEIRPFRQNW-----EEADSMKSSVES-LQNRVTELESVDKSAGQAARNTGLLESQLSRHDQMLSVHDIRLADMDLRFQVLETASYNGVLIWKIRDYKRRKQEAVMGKTLSLYSQPFYTGYFGYKMCARVYLNGDGMGKGTHLSLFFVIMRGEYDALLPWPFKQKVTLMLMDQGS 502
[Close]
BLAST of SMED30020153 vs. Ensembl Mouse
Match: Traf3 (TNF receptor-associated factor 3 [Source:MGI Symbol;Acc:MGI:108041])

HSP 1 Score: 51.2174 bits (121), Expect = 2.182e-6
Identity = 62/267 (23.22%), Postives = 117/267 (43.82%), Query Frame = 2 
Query:  401 ANNQQIPI-EKSSSLQFIN------NFTSTKPGTLSEITVETASSKMELNDE--------ESLKDLLYKNAAKI----KLMDEEIRMLKSVNESLIHMMDHWRTMLDNKLESMNSEMRTTFQDIKLEIEGAAKRKQSQSDESDILSTSISK---------TKLTEPRISLE----RKIAGIYTWNIDRVMEKR-----GKRGSIWSEFFYTRNNHYKMRLQFHPNG-FVCTGTGLSIYLFLYPSETNSKGLWPFKADVTMKIVNKAN 1087
              NQQI   E SS++Q +N      N    K   L   +VE   S   L+++        E  K++L  N +KI    +++D +   LK +++ +     +W      + +SM S + +  Q+   E+E   K     +  + +L + +S+          +L +  +  +        G+  W I     ++     GK  S++S+ FYT    YKM  + + NG  +  GT LS++  +   E ++   WPFK  VT+ ++++ +
Sbjct:  217 GTNQQIKAHEASSAVQHVNLLKEWSNSLEKKVSLLQNESVEKNKSIQSLHNQICSFEIEIERQKEMLRNNESKILHLQRVIDSQAEKLKELDKEIRPFRQNW-----EEADSMKSSVES-LQNRVTELESVDKSAGQAARNTGLLESQLSRHDQMLSVHDIRLADMDLRFQVLETASYNGVLIWKIRDYKRRKQEAVMGKTLSLYSQPFYTGYFGYKMCARVYLNGDGMGKGTHLSLFFVIMRGEYDALLPWPFKQKVTLMLMDQGS 477
[Close]
BLAST of SMED30020153 vs. Ensembl Mouse
Match: Traf3 (TNF receptor-associated factor 3 [Source:MGI Symbol;Acc:MGI:108041])

HSP 1 Score: 51.2174 bits (121), Expect = 2.182e-6
Identity = 62/267 (23.22%), Postives = 117/267 (43.82%), Query Frame = 2 
Query:  401 ANNQQIPI-EKSSSLQFIN------NFTSTKPGTLSEITVETASSKMELNDE--------ESLKDLLYKNAAKI----KLMDEEIRMLKSVNESLIHMMDHWRTMLDNKLESMNSEMRTTFQDIKLEIEGAAKRKQSQSDESDILSTSISK---------TKLTEPRISLE----RKIAGIYTWNIDRVMEKR-----GKRGSIWSEFFYTRNNHYKMRLQFHPNG-FVCTGTGLSIYLFLYPSETNSKGLWPFKADVTMKIVNKAN 1087
              NQQI   E SS++Q +N      N    K   L   +VE   S   L+++        E  K++L  N +KI    +++D +   LK +++ +     +W      + +SM S + +  Q+   E+E   K     +  + +L + +S+          +L +  +  +        G+  W I     ++     GK  S++S+ FYT    YKM  + + NG  +  GT LS++  +   E ++   WPFK  VT+ ++++ +
Sbjct:  217 GTNQQIKAHEASSAVQHVNLLKEWSNSLEKKVSLLQNESVEKNKSIQSLHNQICSFEIEIERQKEMLRNNESKILHLQRVIDSQAEKLKELDKEIRPFRQNW-----EEADSMKSSVES-LQNRVTELESVDKSAGQAARNTGLLESQLSRHDQMLSVHDIRLADMDLRFQVLETASYNGVLIWKIRDYKRRKQEAVMGKTLSLYSQPFYTGYFGYKMCARVYLNGDGMGKGTHLSLFFVIMRGEYDALLPWPFKQKVTLMLMDQGS 477
[Close]
BLAST of SMED30020153 vs. Ensembl Mouse
Match: Traf1 (TNF receptor-associated factor 1 [Source:MGI Symbol;Acc:MGI:101836])

HSP 1 Score: 50.0618 bits (118), Expect = 3.694e-6
Identity = 46/190 (24.21%), Postives = 81/190 (42.63%), Query Frame = 2 
Query:  590 EEIRMLKSVNESLIHMMDHWRTMLDNKLESMNSEMRTTFQDIKLEIEGAAKRKQSQSDESDILSTSISKTKLTEP---------------RISLERKIAGIYTWNIDRVMEKR-----GKRGSIWSEFFYTRNNHYKMRLQFHPNGFVCTGTG----LSIYLFLYPSETNSKGLWPFKADVTMKIVNKAN 1087
            EE+ +   V E L+  ++    +  N +  +N E+  +       +  AA   QSQ D   ILS      +L +                R+  E    G + W I  V ++      G+  S++S  FYT    YK+ L+ + NG    G+G    LS+++ +   E ++   WPF+  VT  ++++ N
Sbjct:  167 EELALQHLVKEKLLAQLEEKLRVFANIVAVLNKEVEAS------HLALAASIHQSQLDREHILSLEQRVVELQQTLAQKDQVLGKLEHSLRLMEEASFDGTFLWKITNVTKRCHESVCGRTVSLFSPAFYTAKYGYKLCLRLYLNG---DGSGKKTHLSLFIVIMRGEYDALLPWPFRNKVTFMLLDQNN 347
[Close]
BLAST of SMED30020153 vs. Ensembl Mouse
Match: Traf1 (TNF receptor-associated factor 1 [Source:MGI Symbol;Acc:MGI:101836])

HSP 1 Score: 50.0618 bits (118), Expect = 3.694e-6
Identity = 46/190 (24.21%), Postives = 81/190 (42.63%), Query Frame = 2 
Query:  590 EEIRMLKSVNESLIHMMDHWRTMLDNKLESMNSEMRTTFQDIKLEIEGAAKRKQSQSDESDILSTSISKTKLTEP---------------RISLERKIAGIYTWNIDRVMEKR-----GKRGSIWSEFFYTRNNHYKMRLQFHPNGFVCTGTG----LSIYLFLYPSETNSKGLWPFKADVTMKIVNKAN 1087
            EE+ +   V E L+  ++    +  N +  +N E+  +       +  AA   QSQ D   ILS      +L +                R+  E    G + W I  V ++      G+  S++S  FYT    YK+ L+ + NG    G+G    LS+++ +   E ++   WPF+  VT  ++++ N
Sbjct:  167 EELALQHLVKEKLLAQLEEKLRVFANIVAVLNKEVEAS------HLALAASIHQSQLDREHILSLEQRVVELQQTLAQKDQVLGKLEHSLRLMEEASFDGTFLWKITNVTKRCHESVCGRTVSLFSPAFYTAKYGYKLCLRLYLNG---DGSGKKTHLSLFIVIMRGEYDALLPWPFRNKVTFMLLDQNN 347
[Close]
BLAST of SMED30020153 vs. TrEMBL
Match: I1ZID7 (TNF receptor associated factor-3 (Fragment) OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 106.686 bits (265), Expect = 7.026e-23
Identity = 70/213 (32.86%), Postives = 118/213 (55.40%), Query Frame = 2 
Query:  683 NSEMRTTFQDIKLEIEGAA---------KRKQSQSDESDILSTSISKTKLT----EPRISLE---------RKI---AGIYTWNIDRVMEKRGKRGSIWSEFFYTRNNHYKMRLQFHPN-GFVCTGTGLSIYLFLYPSETNSKGLWPFKADVTMKIVNKANPSKSKILTKYCHIEKPVINPFKCCASFDFIYQELYLEGLFQDDYFSIECFVN 1243
            N E    F+++K+E+E               +++ + ++I++  +SK +      + RI L+         +KI   +GI++WN++ +  KR +  S++SE+FYT+ + +K+ LQF  N        G +++LF++     SK  WPF+A+VT+KIVN++ P  SKI+TK   IE+P  N +     F F Y EL   GL  +D F+IECFV+
Sbjct:    3 NIECINEFKNLKIEVEDKMVDLVEKIKLAETKTKQECAEIVTAEVSKLRRDFQEFQERIELKVQNIESYLKQKIDETSGIFSWNVNGISAKRKENQSMFSEYFYTKVSKHKVSLQFTANVSNDINEIGFNVFLFIFSGIQESKLNWPFQANVTIKIVNRSVPDSSKIVTKCFRIEEPPCN-YNWSDPFYFCYDELSGAGLLLNDSFTIECFVD 214
[Close]
BLAST of SMED30020153 vs. TrEMBL
Match: T1DF65 (TRAF-2 OS=Dendrocoelum lacteum OX=27895 PE=2 SV=1)

HSP 1 Score: 67.3958 bits (163), Expect = 3.120e-8
Identity = 43/147 (29.25%), Postives = 76/147 (51.70%), Query Frame = 2 
Query:  686 SEMRTTFQDIKLEIEGAAKRKQSQSDESDILSTSISKTKLTEPRISLERKIAGIYTWNIDRVMEK-----RGKRGSIWSEFFYTRNNHYKMRLQFHPNGFVCT-GTGLSIYLFLYPSETNSKGLWPFKADVTMKIVNKANPSKSKIL 1108
            S + T  +D+K +++      +   +E ++   S+S+TK        ER   G YTW ++ +MEK     +G    I SE FY+ N  Y+M L+ +PNG     G  LS+Y  L   + + +  WPF + +++++VN A   + ++L
Sbjct:  281 SSLLTEIEDLKTKLKQFQSMYEKLENERNLAKCSLSETK--------ER--YGEYTWKVENIMEKVNDAKKGVNTQILSESFYSSNPGYRMCLKIYPNGDGSAFGNYLSVYFVLMKGKYDDQIQWPFNSIISIRLVNIARRGEDRVL 417
[Close]
BLAST of SMED30020153 vs. TrEMBL
Match: D5JG60 (TNF receptor-associated factor-2-like protein B OS=Dugesia japonica OX=6161 GN=TRAF-2B PE=2 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 3.267e-7
Identity = 34/90 (37.78%), Postives = 52/90 (57.78%), Query Frame = 2 
Query:  842 GIYTWNIDRVME-----KRGKRGSIWSEFFYTRNNHYKMRLQFHPNG-FVCTGTGLSIYLFLYPSETNSKGLWPFKADVTMKIVNKANPS 1093
            G++ W I+R  E     +RG R SI S++FYT+   YKM ++ +P G  V  GT LS++  L   E +    WPFK  VT+ I+++ N +
Sbjct:  305 GVFIWKIERFSELKSEAERGNRISITSDYFYTKEFGYKMSMKIYPAGDGVGKGTHLSVFFTLMRGEFDDLLQWPFKNKVTLTILDQKNKT 394
[Close]
BLAST of SMED30020153 vs. Ensembl Nematostella
Match: EDO35237 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SM27])

HSP 1 Score: 56.225 bits (134), Expect = 1.498e-8
Identity = 39/116 (33.62%), Postives = 61/116 (52.59%), Query Frame = 2 
Query:  779 STSISKTKLTEP--RISLERKIA--GIYTWNIDRVME-----KRGKRGSIWSEFFYTRNNHYKMRLQFHPNGFVCTGTG----LSIYLFLYPSETNSKGLWPFKADVTMKIVNKAN 1087
            S +I KTKL +   RI +++ I+  G + W ID+  E     K G   SI+S  FYT    YK+  +  PNG    G+     LSI++ L   E +S   WPF+  +T+ ++++ N
Sbjct:  355 SITIHKTKLFQEDLRIQIQQAISYDGTFLWKIDKFTERYAEAKSGNATSIYSSPFYTHRFGYKLCARLFPNG---DGSARDNYLSIFIALMRGEYDSILPWPFQETITITLIDQDN 467
[Close]
BLAST of SMED30020153 vs. Ensembl Nematostella
Match: EDO36181 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SJA2])

HSP 1 Score: 48.1358 bits (113), Expect = 4.775e-7
Identity = 26/84 (30.95%), Postives = 46/84 (54.76%), Query Frame = 2 
Query:  848 YTWNIDRVMEKRGKRGSIWSEFFYTRNNHYKMRLQFHPNGF-VCTGTGLSIYLFLYPSETNSKGLWPFKADVTMKIVNKANPSK 1096
            + W I    ++  K+ +I S  FYT    YKMRL+ H NG     GT LS+++ +   E ++   WPFK  +T+ ++++ +  +
Sbjct:   17 FVWRIRNYNDQYEKKQTISSRLFYTSRYGYKMRLKSHLNGNGDGKGTHLSLFVQITKGEYDALLEWPFKRKITLYLIDQIDQQR 100
[Close]
BLAST of SMED30020153 vs. Ensembl Nematostella
Match: EDO49464 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RG36])

HSP 1 Score: 48.9062 bits (115), Expect = 2.820e-6
Identity = 28/86 (32.56%), Postives = 43/86 (50.00%), Query Frame = 2 
Query:  842 GIYTWNIDRVMEK-----RGKRGSIWSEFFYTRNNHYKMRLQFHPNG-FVCTGTGLSIYLFLYPSETNSKGLWPFKADVTMKIVNK 1081
            G Y W ID    +      GK  SI+S  FY     YK   + +PNG  +  G+ LS++  L   E ++   WPF+  VT K++++
Sbjct:  378 GTYIWKIDEYTRRYQEGVSGKTPSIYSPPFYVGRYGYKACARVYPNGDGMGKGSHLSLFFVLMRGEFDALLPWPFRQKVTFKLLDQ 463
[Close]
BLAST of SMED30020153 vs. Ensembl Nematostella
Match: EDO37750 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SER6])

HSP 1 Score: 48.9062 bits (115), Expect = 3.276e-6
Identity = 29/89 (32.58%), Postives = 45/89 (50.56%), Query Frame = 2 
Query:  854 WNIDRVME-----KRGKRGSIWSEFFYTRNNHYKMRLQFHPNG-FVCTGTGLSIYLFLYPSETNSKGLWPFKADVTMKIVNKANPSKSK 1102
            W ID   E     KR     + S  FYT N  YK ++   P G     GT LS+Y+ L P E +S   WPF+  +T+ +++++   ++K
Sbjct:  317 WRIDDFWEAFDEGKRKPGTELHSPVFYTSNYGYKFKVVLFPYGNGSGEGTHLSLYIRLLPGEYDSLLPWPFEGQITLTLLDQSTDKRAK 405
[Close]
BLAST of SMED30020153 vs. Ensembl Nematostella
Match: EDO48394 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RJK8])

HSP 1 Score: 48.9062 bits (115), Expect = 3.412e-6
Identity = 35/120 (29.17%), Postives = 58/120 (48.33%), Query Frame = 2 
Query:  914 FYTRNNHYKMRLQFHPNGFV-CTGTGLSIYLFLYPSETNSKGLWPFKADVTMKIVNKANPSKSKILTKY---------CHIEKPVINPFKCCASFDFI-YQELYLEGLFQDDYFSIECFV 1240
            FYT    YKMRL+ +PNG+    GT LS+ + +   E ++   WPF+  +T+ ++N++   +S+ +  Y            E+P+ N    C    FI + EL      +DD   IE  +
Sbjct:  368 FYTSRYGYKMRLKAYPNGYGDGKGTHLSLSIRIMKGEYDAILEWPFRRKITLSLINQSEQWRSETVEIYPDDATPDKKHKFERPIENENVGCGYSCFISHMELNSGDYIKDDVIFIEAVI 487
[Close]
BLAST of SMED30020153 vs. Planmine SMEST
Match: SMESG000025120.1 (SMESG000025120.1)

HSP 1 Score: 729.939 bits (1883), Expect = 0.000e+0
Identity = 353/358 (98.60%), Postives = 355/358 (99.16%), Query Frame = 2 
Query:  176 MTDPNIIHTNNDLEFPIGVFGVFRESADGRHEESLSNSMDINSTVNEMLFGIAEPNSISPLKISRANADLWNNLSANNQQIPIEKSSSLQFINNFTSTKPGTLSEITVETASSKMELNDEESLKDLLYKNAAKIKLMDEEIRMLKSVNESLIHMMDHWRTMLDNKLESMNSEMRTTFQDIKLEIEGAAKRKQSQSDESDILSTSISKTKLTEPRISLERKIAGIYTWNIDRVMEKRGKRGSIWSEFFYTRNNHYKMRLQFHPNGFVCTGTGLSIYLFLYPSETNSKGLWPFKADVTMKIVNKANPSKSKILTKYCHIEKPVINPFKCCASFDFIYQELYLEGLFQDDYFSIECFVNVR 1249
            MTDPNIIHTNNDLEFPIGVFGVFRESADGRHEESLSNSMDINSTVNEMLFGIAEPNSISPL   RANADLWNNLSANN+QIPIEKSSSLQFINNFTSTKPGTLSEITVETASSKMELNDEESLKDLLYKNAAKIKLMDEEIRMLKSVNESLIHMMDHWRTMLDNKLESMNSEMRTTFQDIKLEIEGAAKRKQSQSDE+DILSTSISKTKLTEPRISLERKIAGIYTWNIDRVMEKRGKRGSIWSEFFYTRNNHYKMRLQFHPNGFVCTGTGLSIYLFLYPSETNSKGLWPFKADVTMKIVNKANPSKSKILTKYCHIEKPVINPFKCCASFDFIYQELYLEGLFQDDYFSIECFVNVR
Sbjct:    1 MTDPNIIHTNNDLEFPIGVFGVFRESADGRHEESLSNSMDINSTVNEMLFGIAEPNSISPL---RANADLWNNLSANNEQIPIEKSSSLQFINNFTSTKPGTLSEITVETASSKMELNDEESLKDLLYKNAAKIKLMDEEIRMLKSVNESLIHMMDHWRTMLDNKLESMNSEMRTTFQDIKLEIEGAAKRKQSQSDENDILSTSISKTKLTEPRISLERKIAGIYTWNIDRVMEKRGKRGSIWSEFFYTRNNHYKMRLQFHPNGFVCTGTGLSIYLFLYPSETNSKGLWPFKADVTMKIVNKANPSKSKILTKYCHIEKPVINPFKCCASFDFIYQELYLEGLFQDDYFSIECFVNVR 355
[Close]
BLAST of SMED30020153 vs. Planmine SMEST
Match: SMESG000025120.1 (SMESG000025120.1)

HSP 1 Score: 691.034 bits (1782), Expect = 0.000e+0
Identity = 339/358 (94.69%), Postives = 341/358 (95.25%), Query Frame = 2 
Query:  176 MTDPNIIHTNNDLEFPIGVFGVFRESADGRHEESLSNSMDINSTVNEMLFGIAEPNSISPLKISRANADLWNNLSANNQQIPIEKSSSLQFINNFTSTKPGTLSEITVETASSKMELNDEESLKDLLYKNAAKIKLMDEEIRMLKSVNESLIHMMDHWRTMLDNKLESMNSEMRTTFQDIKLEIEGAAKRKQSQSDESDILSTSISKTKLTEPRISLERKIAGIYTWNIDRVMEKRGKRGSIWSEFFYTRNNHYKMRLQFHPNGFVCTGTGLSIYLFLYPSETNSKGLWPFKADVTMKIVNKANPSKSKILTKYCHIEKPVINPFKCCASFDFIYQELYLEGLFQDDYFSIECFVNVR 1249
            MTDPNIIHTNNDLEFPIGVFGVFRESADGRHEESLSNSMDINSTVNEMLFGIAEPNSISPL   RANADLWNNLSANN+QIPIEKSSSLQFINNFTSTKPGTLSEITVETASSKMELNDEESLKDLLYKNAAKIKLMDEEIRMLKSVNESLIHMMDHWRTMLDNKLESMNSEMRTTFQDIKLEIEGAAKRKQSQSDE+DILSTSISKTKLTEPRISLERKIAGIYTWNIDRVMEKRGKRGSIWSEFFYTRNNHYKMRLQFHPNGFVCTGTGLS              LWPFKADVTMKIVNKANPSKSKILTKYCHIEKPVINPFKCCASFDFIYQELYLEGLFQDDYFSIECFVNVR
Sbjct:    1 MTDPNIIHTNNDLEFPIGVFGVFRESADGRHEESLSNSMDINSTVNEMLFGIAEPNSISPL---RANADLWNNLSANNEQIPIEKSSSLQFINNFTSTKPGTLSEITVETASSKMELNDEESLKDLLYKNAAKIKLMDEEIRMLKSVNESLIHMMDHWRTMLDNKLESMNSEMRTTFQDIKLEIEGAAKRKQSQSDENDILSTSISKTKLTEPRISLERKIAGIYTWNIDRVMEKRGKRGSIWSEFFYTRNNHYKMRLQFHPNGFVCTGTGLS--------------LWPFKADVTMKIVNKANPSKSKILTKYCHIEKPVINPFKCCASFDFIYQELYLEGLFQDDYFSIECFVNVR 341
[Close]
BLAST of SMED30020153 vs. Planmine SMEST
Match: SMESG000025120.1 (SMESG000025120.1)

HSP 1 Score: 689.493 bits (1778), Expect = 0.000e+0
Identity = 337/356 (94.66%), Postives = 339/356 (95.22%), Query Frame = 2 
Query:  176 MTDPNIIHTNNDLEFPIGVFGVFRESADGRHEESLSNSMDINSTVNEMLFGIAEPNSISPLKISRANADLWNNLSANNQQIPIEKSSSLQFINNFTSTKPGTLSEITVETASSKMELNDEESLKDLLYKNAAKIKLMDEEIRMLKSVNESLIHMMDHWRTMLDNKLESMNSEMRTTFQDIKLEIEGAAKRKQSQSDESDILSTSISKTKLTEPRISLERKIAGIYTWNIDRVMEKRGKRGSIWSEFFYTRNNHYKMRLQFHPNGFVCTGTGLSIYLFLYPSETNSKGLWPFKADVTMKIVNKANPSKSKILTKYCHIEKPVINPFKCCASFDFIYQELYLEGLFQDDYFSIECFVN 1243
            MTDPNIIHTNNDLEFPIGVFGVFRESADGRHEESLSNSMDINSTVNEMLFGIAEPNSISPL   RANADLWNNLSANN+QIPIEKSSSLQFINNFTSTKPGTLSEITVETASSKMELNDEESLKDLLYKNAAKIKLMDEEIRMLKSVNESLIHMMDHWRTMLDNKLESMNSEMRTTFQDIKLEIEGAAKRKQSQSDE+DILSTSISKTKLTEPRISLERKIAGIYTWNIDRVMEKRGKRGSIWSEFFYTRNNHYKMRLQFHPNGFVCTGTGLS              LWPFKADVTMKIVNKANPSKSKILTKYCHIEKPVINPFKCCASFDFIYQELYLEGLFQDDYFSIECFVN
Sbjct:    1 MTDPNIIHTNNDLEFPIGVFGVFRESADGRHEESLSNSMDINSTVNEMLFGIAEPNSISPL---RANADLWNNLSANNEQIPIEKSSSLQFINNFTSTKPGTLSEITVETASSKMELNDEESLKDLLYKNAAKIKLMDEEIRMLKSVNESLIHMMDHWRTMLDNKLESMNSEMRTTFQDIKLEIEGAAKRKQSQSDENDILSTSISKTKLTEPRISLERKIAGIYTWNIDRVMEKRGKRGSIWSEFFYTRNNHYKMRLQFHPNGFVCTGTGLS--------------LWPFKADVTMKIVNKANPSKSKILTKYCHIEKPVINPFKCCASFDFIYQELYLEGLFQDDYFSIECFVN 339
[Close]
BLAST of SMED30020153 vs. Planmine SMEST
Match: SMESG000024584.1 (SMESG000024584.1)

HSP 1 Score: 106.301 bits (264), Expect = 9.855e-25
Identity = 77/238 (32.35%), Postives = 130/238 (54.62%), Query Frame = 2 
Query:  581 LMDEEIRMLKSVNESLIHMMDHWRTMLDNKLESMNSEMRTTFQDIKLEIEGAAKRKQSQSDESDILSTSISKTKLT----EPRISLE---------RKI---AGIYTWNIDRVMEKRGKRGSIWSEFFYTRNNHYKMRLQFHPN-GFVCTGTGLSIYLFLYPSETNSKGLWPFKADVTMKIVNKANPSKSKILTKYCHIEKPVINPFKCCASFDFIYQELYLEGLFQDDYFSIECFVN 1243
            L++ EI ++K  N   I+   + +  +++K+  +        + IKL      K KQ   + ++I++  +SK +      + RI L+         +KI   +GI++WN++ +  KR +  S++SE+FYT+ + +K+ LQF  N        G +++LF++     SK  WPF+A+VT+KIVN++ P  SKI+TK   IE+P  N +     F F Y EL   GL  +D F+IECFV+
Sbjct:  198 LINSEIEIIKKENIECINEFKNLKIEVEDKMVDL-------VEKIKL---AETKTKQ---ECAEIVTAEVSKLRRDFQEFQERIELKVQNIESYLKQKIDETSGIFSWNVNGISAKRKENQSMFSEYFYTKVSKHKVSLQFTANVSNDINEIGFNVFLFIFSGIQESKLNWPFQANVTIKIVNRSVPDSSKIVTKCFRIEEPPCN-YNWSDPFYFCYDELSGAGLLLNDSFTIECFVD 421
[Close]
BLAST of SMED30020153 vs. Planmine SMEST
Match: SMESG000072057.1 (SMESG000072057.1)

HSP 1 Score: 105.916 bits (263), Expect = 1.363e-24
Identity = 93/332 (28.01%), Postives = 160/332 (48.19%), Query Frame = 2 
Query:  269 EESLSNSMDINSTVNEMLFGIAEPNSIS--PLKISRANADLWN------NLSANNQQIPIEKSSSLQFINNFTSTKPGTLSEITVETASSKMELNDEESLKDLLYKNAAKIKLMDEEIRMLKSVNESLIHMMDHWRTMLDNKLESMNSEMRTTFQDIKLEIEGAAKRKQSQSDESDILSTSISKTKLTEPRISLERKIAGIYTWNIDRVMEKRGKRGSIWSEFFYTRNNHYKMRLQFHPNGFVCTGTGLSIYLFLYPSETNSKGLWPFKADVTMKIVNKANPSKSKILTKYCHIEKPVINPFKCCASFDFIYQELYLEGLFQDDYFSIECFV 1240
            E+ L++SM+ +S +          NSI    L+I +  A+L +       ++A  Q I IE+   L  + N   T    ++EI+ E             L +L+  +  +I  +  EI ++K+  +   +M   +  +  +  E    EM    + ++ EIE  ++ K S+  E + ++    + +L+E   +L   I GI+ W I+++ E + K G IWSE+FY+  N Y+ RL+         G  L I   LY S  +    WPF+A+VT+K+ +K  P+  K +TK C I+KP     +C   F F Y +  L+GL  +D F +EC +
Sbjct:  144 EKHLADSMEKHSKI--------VVNSIETWKLRIEKLEAELASKQNKIEQMTAEMQTIKIEQQELLNEMENLKPTVENKINEISAE-------------LVNLVRDD--RIDSIWNEIDLMKAEGQ---NMSRGFEVIKHDIGEKFKLEMNGKLKAMQAEIECQSEMKMSKLCEREKVNQYDFEKRLSEVEKNLNL-IDGIFMWKINKIEEIK-KCGYIWSEYFYSNANSYRQRLRLDA-----YGDHLWIRFCLYSSPVDIDLDWPFQAEVTLKVASKLWPNSGKTITKNCTIDKPPFGSCECSDEFKFSYSDFILKGLTLNDIFFVECHI 442
The following BLAST results are available for this feature:
BLAST of SMED30020153 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 4
Match NameE-valueIdentityDescription
TRAF34.691e-623.22TNF receptor associated factor 3 [Source:HGNC Symb... [more]
TRAF34.691e-623.22TNF receptor associated factor 3 [Source:HGNC Symb... [more]
TRAF34.918e-623.22TNF receptor associated factor 3 [Source:HGNC Symb... [more]
TRAF34.918e-623.22TNF receptor associated factor 3 [Source:HGNC Symb... [more]
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BLAST of SMED30020153 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020153 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020153 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020153 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ssh29.497e-623.01slingshot protein phosphatase 2 [Source:Xenbase;Ac... [more]
ssh29.618e-623.01slingshot protein phosphatase 2 [Source:Xenbase;Ac... [more]
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BLAST of SMED30020153 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Traf32.011e-623.22TNF receptor-associated factor 3 [Source:MGI Symbo... [more]
Traf32.182e-623.22TNF receptor-associated factor 3 [Source:MGI Symbo... [more]
Traf32.182e-623.22TNF receptor-associated factor 3 [Source:MGI Symbo... [more]
Traf13.694e-624.21TNF receptor-associated factor 1 [Source:MGI Symbo... [more]
Traf13.694e-624.21TNF receptor-associated factor 1 [Source:MGI Symbo... [more]
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BLAST of SMED30020153 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020153 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 3
Match NameE-valueIdentityDescription
I1ZID77.026e-2332.86TNF receptor associated factor-3 (Fragment) OS=Sch... [more]
T1DF653.120e-829.25TRAF-2 OS=Dendrocoelum lacteum OX=27895 PE=2 SV=1[more]
D5JG603.267e-737.78TNF receptor-associated factor-2-like protein B OS... [more]
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BLAST of SMED30020153 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020153 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020153 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020153 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO352371.498e-833.62Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO361814.775e-730.95Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO494642.820e-632.56Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO377503.276e-632.58Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO483943.412e-629.17Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SMED30020153 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020153 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000025120.10.000e+098.60SMESG000025120.1[more]
SMESG000025120.10.000e+094.69SMESG000025120.1[more]
SMESG000025120.10.000e+094.66SMESG000025120.1[more]
SMESG000024584.19.855e-2532.35SMESG000024584.1[more]
SMESG000072057.11.363e-2428.01SMESG000072057.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30020153 ID=SMED30020153|Name=SMED30020153|organism=Schmidtea mediterranea sexual|type=transcript|length=1375bp
CTTATTGAAAATAATTGTTAAATTCATGTACGTAATCTAAGACCATGAGA
TCAGCAGGGTCAGGGTTCATAATCCACAATTTGTTTCTCTATGTAATTCA
TCTGCAAAATACCAATTTAGACTTTCATTCGATATTTATAGAATAAATAT
ATGACTTTTTATACATAACATTTTCATGACCGATCCTAATATTATTCATA
CCAATAATGACTTAGAATTCCCAATTGGAGTTTTCGGAGTATTTCGGGAG
TCAGCAGACGGGCGTCATGAAGAAAGTTTATCAAACTCTATGGACATAAA
TTCCACTGTAAATGAAATGTTATTTGGTATAGCAGAACCAAATTCCATAT
CTCCTTTGAAAATATCGAGAGCCAATGCAGATCTTTGGAATAATTTATCC
GCCAACAACCAGCAAATTCCAATTGAAAAGTCTTCTTCTCTTCAGTTTAT
CAATAATTTTACTTCAACAAAACCCGGAACCTTATCGGAGATTACTGTGG
AAACAGCATCCTCTAAAATGGAACTTAATGACGAAGAATCTTTGAAAGAT
CTTCTATACAAAAACGCTGCTAAAATAAAGTTAATGGACGAAGAGATTAG
GATGTTAAAGAGCGTTAACGAATCCTTAATACATATGATGGATCACTGGC
GAACAATGTTGGATAATAAGTTGGAGTCCATGAATTCAGAAATGCGAACA
ACATTTCAAGACATAAAACTAGAAATTGAAGGAGCAGCGAAAAGAAAACA
ATCTCAAAGTGACGAAAGCGATATCCTCTCAACATCGATTAGCAAGACCA
AGTTAACGGAACCGAGAATATCTTTAGAGAGAAAAATTGCAGGAATTTAC
ACATGGAACATAGATCGAGTCATGGAAAAACGCGGAAAACGTGGGTCAAT
CTGGAGTGAGTTTTTCTATACGAGAAATAACCACTACAAAATGCGGCTGC
AATTTCATCCAAATGGATTTGTCTGCACCGGAACTGGATTAAGTATTTAT
CTATTTCTATATCCAAGCGAGACTAATTCCAAAGGTTTGTGGCCATTCAA
GGCGGACGTGACAATGAAAATTGTCAACAAAGCAAATCCGAGCAAAAGTA
AAATTTTAACGAAGTATTGCCACATTGAGAAACCGGTGATAAACCCCTTT
AAGTGTTGTGCCTCTTTTGATTTCATTTATCAAGAGTTATATTTGGAAGG
ACTTTTTCAAGATGATTATTTTTCAATTGAATGTTTTGTGAATGTGAGAT
AAATATTTTTTATTTAGTTTCACCATTTAATAATCCTCCTTCTGCTCAAA
GTCAAGACCAATTGGAGGGAATTATATAAATTCTGACCTTGTTTGAGCAT
AACGAAACCATCTATTTGATATGTC
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protein sequence of SMED30020153-orf-1

>SMED30020153-orf-1 ID=SMED30020153-orf-1|Name=SMED30020153-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=359bp
MTDPNIIHTNNDLEFPIGVFGVFRESADGRHEESLSNSMDINSTVNEMLF
GIAEPNSISPLKISRANADLWNNLSANNQQIPIEKSSSLQFINNFTSTKP
GTLSEITVETASSKMELNDEESLKDLLYKNAAKIKLMDEEIRMLKSVNES
LIHMMDHWRTMLDNKLESMNSEMRTTFQDIKLEIEGAAKRKQSQSDESDI
LSTSISKTKLTEPRISLERKIAGIYTWNIDRVMEKRGKRGSIWSEFFYTR
NNHYKMRLQFHPNGFVCTGTGLSIYLFLYPSETNSKGLWPFKADVTMKIV
NKANPSKSKILTKYCHIEKPVINPFKCCASFDFIYQELYLEGLFQDDYFS
IECFVNVR*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000034epidermis
PLANA:0000070intestinal phagocyte
PLANA:0000103central nervous system
Vocabulary: INTERPRO
TermDefinition
IPR008974TRAF-like
IPR002083MATH/TRAF_dom
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
GO:0004842ubiquitin-protein transferase activity
GO:0008270zinc ion binding
GO:0046872metal ion binding
Vocabulary: biological process
TermDefinition
GO:0016567protein ubiquitination
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008974TRAF-likeGENE3DG3DSA:2.60.210.10coord: 196..355
e-value: 8.6E-19
score: 69.7
IPR008974TRAF-likeSUPERFAMILYSSF49599TRAF domain-likecoord: 220..354
IPR002083MATH/TRAF domainPROSITEPS50144MATHcoord: 219..352
score: 11.8
IPR002083MATH/TRAF domainCDDcd00121MATHcoord: 220..352
e-value: 8.17771E-14
score: 65.4782