ATP-dependent DNA helicase PIF1
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30020142 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 7
Homology
BLAST of ATP-dependent DNA helicase PIF1 vs. Ensembl Human
Match: PIF1 (PIF1 5'-to-3' DNA helicase [Source:HGNC Symbol;Acc:HGNC:26220]) HSP 1 Score: 377.481 bits (968), Expect = 3.828e-121 Identity = 234/612 (38.24%), Postives = 338/612 (55.23%), Query Frame = 3
Query: 243 YPLVDMQIFERFVTEGKATIKLPK------SSVQIMINNCPPGDLKLFLSTLKYKTKIAVNTVQXXXXXXXXXXXXPRSISSLDTISPIRKSDKFKLQNKALENHFPDIN----PITPEKKRPLRDINEDFNTPPNKKLKIKQYTSTPNAKTSIKEIDNIFDSSEKLKKSATEFDVSLNDSGLTEYYQLSNEQRKIIECVRSGENVFYTGSAGTGKTYLLKKIIGTLVPQVXXXXXXXXXXXXLIDGCTLHNFAGIGLKMESLTASANIIMRKPQLLFQWRKCKHLIIDEISLISAEFFDHLDQVARVVRNNIKVPFGGIQLIISGDFFQLPPILPRNADKHYKFVFQSKVFEQAFKEIHFLTEVKRQSDQEFVAILSEIRMGRCSEATEKRLRTTANQNIERNGIIPTQLCTHKDDVESKNQSNLNSLPGEPEDFFAIDSIPGHRNTVDSVCMVPFKLTLKVGAQVMLLKNIDIAGGLANGSRGVVVGFEPAIKNRYPLVRFSSGKVYTMKPESFSVSISSSGQTLLIARSQIPLKLAYSISIHKSQGMTLDCVDLYLANTFECGQAYVALSRCKSLDSLRVMSWSNHCVKANHDVIKFFNKYQTDDILGL 2048
+PL ++F RF G++T++LP +VQ+++++CPP D A + L PR TISP++ ++ +L+ PD P+ P+ E P L +K+ S P+ K QLS EQ ++ V G+++F+TGSAGTGK+YLLK+I+G+L P T TASTG+AA I G TLH FAGIG L A + ++P + W C+ L+IDEIS++ A+ FD L+ VAR VR K PFGGIQLII GDF QLPP+ + +F FQSK +++ LT+V RQ+DQ F+++L +R+GRCS+ ++L+ TA+ + R+GI+ T+LCTH+DDV N+ L LPG+ F A+DS P +T+D+ C V L LK+GAQVML+KN+ ++ GL NG+RGVVVGFE A P VRF G + + ++V ++ GQ L++R Q+PL+LA+++SIHKSQGMTLDCV++ L F GQAYVALSR +SL LRV+ + V+ + V+ F+ + L L
Sbjct: 69 FPLRAARLFTRFAEAGRSTLRLPAHDTPGAGAVQLLLSDCPP-DRLRRFLRTLRLKLAAAPGPGPASARAQLLGPRPRDFV---TISPVQPEER-RLRAA---TRVPDTTLVKRPVEPQAG------AEPSTEAPRWPLPVKRL-SLPSTKP-----------------------------------QLSEEQAAVLRAVLKGQSIFFTGSAGTGKSYLLKRILGSLPPTGTVATASTGVAACHIGGTTLHAFAGIGSGQAPL-AQCVALAQRPGVRQGWLNCQRLVIDEISMVEADLFDKLEAVARAVRQQNK-PFGGIQLIICGDFLQLPPVT--KGSQPPRFCFQSKSWKRCVPVTLELTKVWRQADQTFISLLQAVRLGRCSDEVTRQLQATASHKVGRDGIVATRLCTHQDDVALTNERRLQELPGKVHRFEAMDSNPELASTLDAQCPVSQLLQLKLGAQVMLVKNLSVSRGLVNGARGVVVGFE-AEGRGLPQVRFLCGVTEVIHADRWTVQ-ATGGQ--LLSRQQLPLQLAWAMSIHKSQGMTLDCVEISLGRVFASGQAYVALSRARSLQGLRVLDFDPMAVRCDPRVLHFYATLRRGRSLSL 622
BLAST of ATP-dependent DNA helicase PIF1 vs. Ensembl Human
Match: PIF1 (PIF1 5'-to-3' DNA helicase [Source:HGNC Symbol;Acc:HGNC:26220]) HSP 1 Score: 377.481 bits (968), Expect = 3.828e-121 Identity = 234/612 (38.24%), Postives = 338/612 (55.23%), Query Frame = 3
Query: 243 YPLVDMQIFERFVTEGKATIKLPK------SSVQIMINNCPPGDLKLFLSTLKYKTKIAVNTVQXXXXXXXXXXXXPRSISSLDTISPIRKSDKFKLQNKALENHFPDIN----PITPEKKRPLRDINEDFNTPPNKKLKIKQYTSTPNAKTSIKEIDNIFDSSEKLKKSATEFDVSLNDSGLTEYYQLSNEQRKIIECVRSGENVFYTGSAGTGKTYLLKKIIGTLVPQVXXXXXXXXXXXXLIDGCTLHNFAGIGLKMESLTASANIIMRKPQLLFQWRKCKHLIIDEISLISAEFFDHLDQVARVVRNNIKVPFGGIQLIISGDFFQLPPILPRNADKHYKFVFQSKVFEQAFKEIHFLTEVKRQSDQEFVAILSEIRMGRCSEATEKRLRTTANQNIERNGIIPTQLCTHKDDVESKNQSNLNSLPGEPEDFFAIDSIPGHRNTVDSVCMVPFKLTLKVGAQVMLLKNIDIAGGLANGSRGVVVGFEPAIKNRYPLVRFSSGKVYTMKPESFSVSISSSGQTLLIARSQIPLKLAYSISIHKSQGMTLDCVDLYLANTFECGQAYVALSRCKSLDSLRVMSWSNHCVKANHDVIKFFNKYQTDDILGL 2048
+PL ++F RF G++T++LP +VQ+++++CPP D A + L PR TISP++ ++ +L+ PD P+ P+ E P L +K+ S P+ K QLS EQ ++ V G+++F+TGSAGTGK+YLLK+I+G+L P T TASTG+AA I G TLH FAGIG L A + ++P + W C+ L+IDEIS++ A+ FD L+ VAR VR K PFGGIQLII GDF QLPP+ + +F FQSK +++ LT+V RQ+DQ F+++L +R+GRCS+ ++L+ TA+ + R+GI+ T+LCTH+DDV N+ L LPG+ F A+DS P +T+D+ C V L LK+GAQVML+KN+ ++ GL NG+RGVVVGFE A P VRF G + + ++V ++ GQ L++R Q+PL+LA+++SIHKSQGMTLDCV++ L F GQAYVALSR +SL LRV+ + V+ + V+ F+ + L L
Sbjct: 69 FPLRAARLFTRFAEAGRSTLRLPAHDTPGAGAVQLLLSDCPP-DRLRRFLRTLRLKLAAAPGPGPASARAQLLGPRPRDFV---TISPVQPEER-RLRAA---TRVPDTTLVKRPVEPQAG------AEPSTEAPRWPLPVKRL-SLPSTKP-----------------------------------QLSEEQAAVLRAVLKGQSIFFTGSAGTGKSYLLKRILGSLPPTGTVATASTGVAACHIGGTTLHAFAGIGSGQAPL-AQCVALAQRPGVRQGWLNCQRLVIDEISMVEADLFDKLEAVARAVRQQNK-PFGGIQLIICGDFLQLPPVT--KGSQPPRFCFQSKSWKRCVPVTLELTKVWRQADQTFISLLQAVRLGRCSDEVTRQLQATASHKVGRDGIVATRLCTHQDDVALTNERRLQELPGKVHRFEAMDSNPELASTLDAQCPVSQLLQLKLGAQVMLVKNLSVSRGLVNGARGVVVGFE-AEGRGLPQVRFLCGVTEVIHADRWTVQ-ATGGQ--LLSRQQLPLQLAWAMSIHKSQGMTLDCVEISLGRVFASGQAYVALSRARSLQGLRVLDFDPMAVRCDPRVLHFYATLRRGRSLSL 622
BLAST of ATP-dependent DNA helicase PIF1 vs. Ensembl Human
Match: PIF1 (PIF1 5'-to-3' DNA helicase [Source:HGNC Symbol;Acc:HGNC:26220]) HSP 1 Score: 377.481 bits (968), Expect = 2.339e-120 Identity = 234/612 (38.24%), Postives = 347/612 (56.70%), Query Frame = 3
Query: 243 YPLVDMQIFERFVTEGKATIKLPK------SSVQIMINNCPPGDLKLFLSTLKYKTKIAVNTVQXXXXXXXXXXXXPRSISSLDTISPIRKSDKFKLQNKALENHFPDINPITPEKKRPLRDINEDFNTPPNKKLKIKQYTSTPNAKTSIKEIDNIFDSSEKLKKSATEFDVSLNDSGL-TEYYQLSNEQRKIIECVRSGENVFYTGSAGTGKTYLLKKIIGTLVPQVXXXXXXXXXXXXLIDGCTLHNFAGIGLKMESLTASANIIMRKPQLLFQWRKCKHLIIDEISLISAEFFDHLDQVARVVRNNIKVPFGGIQLIISGDFFQLPPILPRNADKHYKFVFQSKVFEQAFKEIHFLTEVKRQSDQEFVAILSEIRMGRCSEATEKRLRTTANQNIERNGIIPTQLCTHKDDVESKNQSNLNSLPGEPEDFFAIDSIPGHRNTVDSVCMVPFKLTLKVGAQVMLLKNIDIAGGLANGSRGVVVGFEPAIKNRYPLVRFSSGKVYTMKPESFSVSISSSGQTLLIARSQIPLKLAYSISIHKSQGMTLDCVDLYLANTFECGQAYVALSRCKSLDSLRVMSWSNHCVKANHDVIKFFNKYQTDDILGLYED 2057
+PL ++F RF G++T++LP +VQ+++++CPP L+ FL TL+ K A P S+ + R D F I+P+ PE++R ++ T P+ + ++ + + A + + + L + QLS EQ ++ V G+++F+TGSAGTGK+YLLK+I+G+L P T TASTG+AA I G TLH FAGIG L A + ++P + W C+ L+IDEIS++ A+ FD L+ VAR VR K PFGGIQLII GDF QLPP+ + +F FQSK +++ LT+V RQ+DQ F+++L +R+GRCS+ ++L+ TA+ + R+GI+ T+LCTH+DDV N+ L LPG+ F A+DS P +T+D+ C V L LK+GAQVML+KN+ ++ GL NG+RGVVVGFE A P VRF G + + ++V ++ GQ L++R Q+PL+LA+++SIHKSQGMTLDCV++ L F GQAYVALSR +SL LRV+ + V+ + V+ F+ + L L +
Sbjct: 69 FPLRAARLFTRFAEAGRSTLRLPAHDTPGAGAVQLLLSDCPPDRLRRFLRTLRLKLAAAPG---------------PGPASARAQLLGPRPRD------------FVTISPVQPEERR------------------LRAATRVPDTTLVKRPVEP--QAGAEPSTEAPRWPLPVKRLSLPSTKPQLSEEQAAVLRAVLKGQSIFFTGSAGTGKSYLLKRILGSLPPTGTVATASTGVAACHIGGTTLHAFAGIGSGQAPL-AQCVALAQRPGVRQGWLNCQRLVIDEISMVEADLFDKLEAVARAVRQQNK-PFGGIQLIICGDFLQLPPVT--KGSQPPRFCFQSKSWKRCVPVTLELTKVWRQADQTFISLLQAVRLGRCSDEVTRQLQATASHKVGRDGIVATRLCTHQDDVALTNERRLQELPGKVHRFEAMDSNPELASTLDAQCPVSQLLQLKLGAQVMLVKNLSVSRGLVNGARGVVVGFE-AEGRGLPQVRFLCGVTEVIHADRWTVQ-ATGGQ--LLSRQQLPLQLAWAMSIHKSQGMTLDCVEISLGRVFASGQAYVALSRARSLQGLRVLDFDPMAVRCDPRVLHFYATLRRGRSLSLAAE 625
BLAST of ATP-dependent DNA helicase PIF1 vs. Ensembl Celegans
Match: pif-1 (ATP-dependent DNA helicase PIF1 [Source:UniProtKB/TrEMBL;Acc:V6CIU3]) HSP 1 Score: 343.969 bits (881), Expect = 2.198e-108 Identity = 231/626 (36.90%), Postives = 346/626 (55.27%), Query Frame = 3
Query: 186 MYVRITLISTSKKVTGQYGYPLVDMQIFERFVTEGKATIKLPKSSVQIMINNCPPGDLKLFLSTLKYKTKIAVNTVQXXXXXXXXXXXXPRSISSLD--TISPIRKSDKFKLQNKALENHFPDINPITPEKKRPLRDINEDF--NTPPNKKLKIKQYTSTPNAKTSIKEIDNIFDSSEKLKKSATEFDVSLNDS-GLTEYYQLSNEQRKIIECV-RSGENVFYTGSAGTGKTYLLKKIIGTLVPQVXXXXXXXXXXXXLIDGCTLHNFAGIGLKMESLTASANIIMRKPQLLFQWRKCKHLIIDEISLISAEFFDHLDQVARVVRNNIKVPFGGIQLIISGDFFQLPPILPRNADKHYKFVFQSKVFEQAFKEIHFLTEVKRQSDQEFVAILSEIRMGRCSEATEKRLRTTANQNIERNGIIPTQLCTHKDDVESKNQSNLNSLPGEPEDFFAID--SIPGHRNTVDSVCMVPFKLTLKVGAQVMLLKNIDIAGGLANGSRGVVVGFEPAIKNRYPLVRFSSGKVYTM--KPESFSVSISSSGQTLLIARSQIPLKLAYSISIHKSQGMTLDCVDLYLANTFECGQAYVALSRCKSLDSLRVMSWSNHCVKANHDVIKFFNKYQTD 2033
++++I L +T+ Y + D + + V GK T+++P S+ + NC P L +F+ +L+ K I + P S L TIS +RK K + + AL P TP+++ ++ N N+ + +K+ TS F ++ K S D+ ++E QLS+EQ+ ++ CV S +VF+TGSAGTGK+ +L++II L T ITA+TG+AAS I G TLH F G + + ++R+ ++ QW++C HLIIDEIS+I +FF+ L+ VAR VRNN K PFGGIQLII+GDFFQLPP+ + F F+S+ + + ++ L VKRQ+D FV IL+ +R+G+C + L+ ++ + +IPT+LCTH DD + N S++ + G+ + F A D S H + + KL LKVGAQVML+KNID+ GL NGSRG V F +N P++RF S ++ + FSV I S L+ R Q+PL+LA++ISIHKSQGMTLDC ++ L F GQAYVALSR +SL ++R++ + CV+AN VI F+ + +
Sbjct: 61 IHLQIELKTTATGQPAVVCYDVTDAVVHLQSVANGKCTVEIPSLSLMFQMFNCAPRKLNVFMKSLQAKLDIMKMEKSPISAVPRQFSRPPAVFSVLSPLTISEMRKVKKLR-EPSALAR--PSKEATTPKRRTSSMNLLAGGLENRIMNRSIGLKRTTS--------------FARDDREKAETLVSLKSFKDAPAISERIQLSDEQKSVVRCVINSRTSVFFTGSAGTGKSVILRRIIEMLPAGNTYITAATGVAASQIGGITLHAFCGFRYENSTPEQCLKQVLRQNHMVRQWKQCSHLIIDEISMIDRDFFEALEYVARTVRNNDK-PFGGIQLIITGDFFQLPPV----SKDEPVFCFESEAWSRCIQKTIVLKNVKRQNDNVFVKILNNVRVGKCDFKSADILKESSKNQFP-SSVIPTKLCTHSDDADRINSSSIETTQGDAKTFHAYDDESFDTH---AKARTLAQKKLVLKVGAQVMLIKNIDVIKGLCNGSRGFVEKFS---ENGNPMIRFVSQADASIEIRRSKFSVRIPGSDAPLI--RRQLPLQLAWAISIHKSQGMTLDCAEISLERVFADGQAYVALSRARSLAAIRIIGFDASCVRANSKVIDFYKSIEAE 655
BLAST of ATP-dependent DNA helicase PIF1 vs. Ensembl Celegans
Match: pif-1 (ATP-dependent DNA helicase PIF1 [Source:UniProtKB/TrEMBL;Acc:V6CIU3]) HSP 1 Score: 327.405 bits (838), Expect = 3.415e-103 Identity = 220/575 (38.26%), Postives = 321/575 (55.83%), Query Frame = 3
Query: 339 NCPPGDLKLFLSTLKYKTKIAVNTVQXXXXXXXXXXXXPRSISSLD--TISPIRKSDKFKLQNKALENHFPDINPITPEKKRPLRDINEDF--NTPPNKKLKIKQYTSTPNAKTSIKEIDNIFDSSEKLKKSATEFDVSLNDS-GLTEYYQLSNEQRKIIECV-RSGENVFYTGSAGTGKTYLLKKIIGTLVPQVXXXXXXXXXXXXLIDGCTLHNFAGIGLKMESLTASANIIMRKPQLLFQWRKCKHLIIDEISLISAEFFDHLDQVARVVRNNIKVPFGGIQLIISGDFFQLPPILPRNADKHYKFVFQSKVFEQAFKEIHFLTEVKRQSDQEFVAILSEIRMGRCSEATEKRLRTTANQNIERNGIIPTQLCTHKDDVESKNQSNLNSLPGEPEDFFAID--SIPGHRNTVDSVCMVPFKLTLKVGAQVMLLKNIDIAGGLANGSRGVVVGFEPAIKNRYPLVRFSSGKVYTM--KPESFSVSISSSGQTLLIARSQIPLKLAYSISIHKSQGMTLDCVDLYLANTFECGQAYVALSRCKSLDSLRVMSWSNHCVKANHDVIKFFNKYQTD 2033
NC P L +F+ +L+ K I + P S L TIS +RK K + + AL P TP+++ ++ N N+ + +K+ TS F ++ K S D+ ++E QLS+EQ+ ++ CV S +VF+TGSAGTGK+ +L++II L T ITA+TG+AAS I G TLH F G + + ++R+ ++ QW++C HLIIDEIS+I +FF+ L+ VAR VRNN K PFGGIQLII+GDFFQLPP+ + F F+S+ + + ++ L VKRQ+D FV IL+ +R+G+C + L+ ++ + +IPT+LCTH DD + N S++ + G+ + F A D S H + + KL LKVGAQVML+KNID+ GL NGSRG V F +N P++RF S ++ + FSV I S L+ R Q+PL+LA++ISIHKSQGMTLDC ++ L F GQAYVALSR +SL ++R++ + CV+AN VI F+ + +
Sbjct: 6 NCAPRKLNVFMKSLQAKLDIMKMEKSPISAVPRQFSRPPAVFSVLSPLTISEMRKVKKLR-EPSALAR--PSKEATTPKRRTSSMNLLAGGLENRIMNRSIGLKRTTS--------------FARDDREKAETLVSLKSFKDAPAISERIQLSDEQKSVVRCVINSRTSVFFTGSAGTGKSVILRRIIEMLPAGNTYITAATGVAASQIGGITLHAFCGFRYENSTPEQCLKQVLRQNHMVRQWKQCSHLIIDEISMIDRDFFEALEYVARTVRNNDK-PFGGIQLIITGDFFQLPPV----SKDEPVFCFESEAWSRCIQKTIVLKNVKRQNDNVFVKILNNVRVGKCDFKSADILKESSKNQFP-SSVIPTKLCTHSDDADRINSSSIETTQGDAKTFHAYDDESFDTH---AKARTLAQKKLVLKVGAQVMLIKNIDVIKGLCNGSRGFVEKFS---ENGNPMIRFVSQADASIEIRRSKFSVRIPGSDAPLI--RRQLPLQLAWAISIHKSQGMTLDCAEISLERVFADGQAYVALSRARSLAAIRIIGFDASCVRANSKVIDFYKSIEAE 549
BLAST of ATP-dependent DNA helicase PIF1 vs. Ensembl Celegans
Match: pif-1 (ATP-dependent DNA helicase PIF1 [Source:UniProtKB/TrEMBL;Acc:V6CIU3]) HSP 1 Score: 283.108 bits (723), Expect = 1.493e-88 Identity = 158/367 (43.05%), Postives = 226/367 (61.58%), Query Frame = 3
Query: 945 IDGCTLHNFAGIGLKMESLTASANIIMRKPQLLFQWRKCKHLIIDEISLISAEFFDHLDQVARVVRNNIKVPFGGIQLIISGDFFQLPPILPRNADKHYKFVFQSKVFEQAFKEIHFLTEVKRQSDQEFVAILSEIRMGRCSEATEKRLRTTANQNIERNGIIPTQLCTHKDDVESKNQSNLNSLPGEPEDFFAID--SIPGHRNTVDSVCMVPFKLTLKVGAQVMLLKNIDIAGGLANGSRGVVVGFEPAIKNRYPLVRFSSGKVYTM--KPESFSVSISSSGQTLLIARSQIPLKLAYSISIHKSQGMTLDCVDLYLANTFECGQAYVALSRCKSLDSLRVMSWSNHCVKANHDVIKFFNKYQTD 2033
I G TLH F G + + ++R+ ++ QW++C HLIIDEIS+I +FF+ L+ VAR VRNN K PFGGIQLII+GDFFQLPP+ + F F+S+ + + ++ L VKRQ+D FV IL+ +R+G+C + L+ ++ +N + +IPT+LCTH DD + N S++ + G+ + F A D S H + + KL LKVGAQVML+KNID+ GL NGSRG V F +N P++RF S ++ + FSV I S L+ R Q+PL+LA++ISIHKSQGMTLDC ++ L F GQAYVALSR +SL ++R++ + CV+AN VI F+ + +
Sbjct: 20 IGGITLHAFCGFRYENSTPEQCLKQVLRQNHMVRQWKQCSHLIIDEISMIDRDFFEALEYVARTVRNNDK-PFGGIQLIITGDFFQLPPV----SKDEPVFCFESEAWSRCIQKTIVLKNVKRQNDNVFVKILNNVRVGKCDFKSADILKESS-KNQFPSSVIPTKLCTHSDDADRINSSSIETTQGDAKTFHAYDDESFDTH---AKARTLAQKKLVLKVGAQVMLIKNIDVIKGLCNGSRGFVEKFS---ENGNPMIRFVSQADASIEIRRSKFSVRIPGSDAPLI--RRQLPLQLAWAISIHKSQGMTLDCAEISLERVFADGQAYVALSRARSLAAIRIIGFDASCVRANSKVIDFYKSIEAE 372
BLAST of ATP-dependent DNA helicase PIF1 vs. Ensembl Celegans
Match: ZK250.9 (ATP-dependent DNA helicase [Source:UniProtKB/TrEMBL;Acc:G5ED32]) HSP 1 Score: 97.4413 bits (241), Expect = 1.629e-20 Identity = 94/337 (27.89%), Postives = 167/337 (49.55%), Query Frame = 3
Query: 1068 LIIDEISLISAEFFDHLDQVARVVRNNIKVPFGGIQLIISGDFFQLPPILPRN--ADKHYKFVFQSKVFEQAFKEIHFLTEVKRQS-DQEFVAILSEIRMGRCSEATEK---RLRTTANQNI--ERNGII--PTQ-------LCTHKD-DVESKNQSNLNSLPGEPEDFFAIDS-IPGHR-----------NTVDSVCMVPFKLTLKVGAQVMLLKNIDIAGGLANGSRGVVVGFEPAIKNRYPLVRFSSGKV---YTMKPESFSVSISSSGQTLLIARSQIPLKLAYSISIHKSQGMTLDCVDLYL-ANTFECGQAYVALSRCKSLDSLRVMSWSN 1976
I DE S++ D +D + R + I PFGG LI+ GDF Q+ P++ R+ AD+ + +S ++ + F+ +H ++ ++ S D +++ L + G +++ K L + NI E G + PT + T K+ DV N N + GE + + D I H+ N + + P L LK G+ ++LL+N+D++ GL NGSR +V + + RF++G+ +T+ P + + R+Q P++L++++SI+K+QG + + L++ + F GQ YVALSR ++ + L V S SN
Sbjct: 1127 FIWDEASMVPKTALDTVDVLLRDL-TKIDQPFGGKILILGGDFRQILPVVERSSRADQVDACIKRSPLWTE-FQILHLISNMRVTSGDSDWIQFLLNVGDGSANDSDSKVTLPLSVMCDHNIVEEVFGAVIDPTTSDPCDNVILTPKNVDVAQLNDDVHNRMVGEERIYLSRDEVIVEHQADTMHYPTEFLNKMSPSSLPPHILKLKKGSVIILLRNLDVSAGLCNGSRFIV----ETLASHSLGCRFATGERKGHFTIIPR--IDCYDDKNISFQLRRTQFPVRLSFALSINKAQGQSFSKIGLWIPTDVFTHGQLYVALSRVRTKEGLIVKSSSN 1455
BLAST of ATP-dependent DNA helicase PIF1 vs. Ensembl Celegans
Match: Y16E11A.2 (ATP-dependent DNA helicase [Source:UniProtKB/TrEMBL;Acc:G5ED32]) HSP 1 Score: 97.4413 bits (241), Expect = 1.629e-20 Identity = 94/337 (27.89%), Postives = 167/337 (49.55%), Query Frame = 3
Query: 1068 LIIDEISLISAEFFDHLDQVARVVRNNIKVPFGGIQLIISGDFFQLPPILPRN--ADKHYKFVFQSKVFEQAFKEIHFLTEVKRQS-DQEFVAILSEIRMGRCSEATEK---RLRTTANQNI--ERNGII--PTQ-------LCTHKD-DVESKNQSNLNSLPGEPEDFFAIDS-IPGHR-----------NTVDSVCMVPFKLTLKVGAQVMLLKNIDIAGGLANGSRGVVVGFEPAIKNRYPLVRFSSGKV---YTMKPESFSVSISSSGQTLLIARSQIPLKLAYSISIHKSQGMTLDCVDLYL-ANTFECGQAYVALSRCKSLDSLRVMSWSN 1976
I DE S++ D +D + R + I PFGG LI+ GDF Q+ P++ R+ AD+ + +S ++ + F+ +H ++ ++ S D +++ L + G +++ K L + NI E G + PT + T K+ DV N N + GE + + D I H+ N + + P L LK G+ ++LL+N+D++ GL NGSR +V + + RF++G+ +T+ P + + R+Q P++L++++SI+K+QG + + L++ + F GQ YVALSR ++ + L V S SN
Sbjct: 1127 FIWDEASMVPKTALDTVDVLLRDL-TKIDQPFGGKILILGGDFRQILPVVERSSRADQVDACIKRSPLWTE-FQILHLISNMRVTSGDSDWIQFLLNVGDGSANDSDSKVTLPLSVMCDHNIVEEVFGAVIDPTTSDPCDNVILTPKNVDVAQLNDDVHNRMVGEERIYLSRDEVIVEHQADTMHYPTEFLNKMSPSSLPPHILKLKKGSVIILLRNLDVSAGLCNGSRFIV----ETLASHSLGCRFATGERKGHFTIIPR--IDCYDDKNISFQLRRTQFPVRLSFALSINKAQGQSFSKIGLWIPTDVFTHGQLYVALSRVRTKEGLIVKSSSN 1455
BLAST of ATP-dependent DNA helicase PIF1 vs. Ensembl Fly
Match: Pif1 (gene:FBgn0031540 transcript:FBtr0077569) HSP 1 Score: 372.859 bits (956), Expect = 1.792e-119 Identity = 236/599 (39.40%), Postives = 338/599 (56.43%), Query Frame = 3
Query: 264 IFERFVTEGKATIKLPKSSVQIMINNCPPGDLKLFLSTLKYKTKIAVNTVQ---XXXXXXXXXXXXPRSISSLDTISPIRKSDKFKLQNKALENHFPDINPITPE--KKRPLRDINEDFNTPPNKKLKIKQYTSTPNAKTSIKEIDNIFDSSEKLKKSATEFDVSLNDSGLTEYYQLSNEQRKIIECVRSGENVFYTGSAGTGKTYLLKKIIGTLVPQVXXXXXXXXXXXXLIDGCTLHNFAGIGLKMESLTASANIIMRKPQLLFQWRKCKHLIIDEISLISAEFFDHLDQVARVVRNNIKVPFGGIQLIISGDFFQLPPILPRN------ADKHYKFVFQSKVFEQAFKEIHFLTEVKRQSDQEFVAILSEIRMGRCSEATEKRLRTTANQNIERNGIIPTQLCTHKDDVESKNQSNLNSLPGEPEDFFAIDSIPGHRNTVDSVCMVPFKLTLKVGAQVMLLKNIDIAGGLANGSRGVVVGFEPAIKNRYPLVRFSSGKVYTMKPESFSVSISSSGQTLLIARSQIPLKLAYSISIHKSQGMTLDCVDLYLANTFECGQAYVALSRCKSLDSLRVMSWSNHCVKANHDVIKFFNKYQ 2027
+ ++F++EGKAT + ++N PPG L FL T+ K Q +KK + ++ SS + +SP+ ++ + KA + +P+ KKR ++ E+ K+ + P AK KL S T+ E +LS EQ +++ SG++VF+TGSAGTGK++LL++II L P T TASTG+AA LI G TLH FAGIG ++ + R P WRKCK LIIDEIS++ +FF+ ++ VAR +R N + PFGGIQLI+ GDF QLPP++ + A +F FQS +E + ++ L +V RQSD EFV IL+ +R+G +++ RL T+ Q IE NGI+ TQLC+H +D S N+S L +L G+ F A DS T+D P +L LKV AQVMLLKNI+I+ GL NG+RGVVV E + P+VRF + + Y K E + + +S I R Q+PLKLA++ SIHKSQG+TLDCV++ L+ FE GQAYVALSR KSL S+R++ + V AN V++F+ ++
Sbjct: 65 VHKKFMSEGKATFNFKAEKCTLYLSNAPPGTLMFFLRTIFVKMNGNEGGSQPDDATLQKKLREHLMSGKPSSFNDVSPVTTAEMILARKKAGLLSKGSVTTPSPQGAKKRRFEELKEE-----------KERGTMPAAK--------------KLYASTTD-----------ESLRLSEEQMEVLRACTSGKSVFFTGSAGTGKSFLLRRIISALPPDGTVATASTGVAACLIGGTTLHAFAGIGGGDATMQRCLELASR-PANAQTWRKCKRLIIDEISMVDGQFFEKIEAVARHIRRNDR-PFGGIQLILCGDFLQLPPVIKGDFGAAPTATPQQRFCFQSSAWETCIQCVYELKQVHRQSDPEFVKILNHLRIGHVNDSITSRLAATSKQKIEGNGILATQLCSHTNDANSINESKLENLDGDKILFKADDSDASMTRTLDQQIQAPSQLYLKVNAQVMLLKNINISNGLVNGARGVVVRMEKDL----PVVRFKNNQEYVCKHERWIIKTASGNH---ITRRQVPLKLAWAFSIHKSQGLTLDCVEMSLSKVFEAGQAYVALSRAKSLQSIRILDFDAKQVWANPHVLQFYKGFR 618
BLAST of ATP-dependent DNA helicase PIF1 vs. Ensembl Fly
Match: Pif1 (gene:FBgn0031540 transcript:FBtr0343239) HSP 1 Score: 372.859 bits (956), Expect = 1.792e-119 Identity = 236/599 (39.40%), Postives = 338/599 (56.43%), Query Frame = 3
Query: 264 IFERFVTEGKATIKLPKSSVQIMINNCPPGDLKLFLSTLKYKTKIAVNTVQ---XXXXXXXXXXXXPRSISSLDTISPIRKSDKFKLQNKALENHFPDINPITPE--KKRPLRDINEDFNTPPNKKLKIKQYTSTPNAKTSIKEIDNIFDSSEKLKKSATEFDVSLNDSGLTEYYQLSNEQRKIIECVRSGENVFYTGSAGTGKTYLLKKIIGTLVPQVXXXXXXXXXXXXLIDGCTLHNFAGIGLKMESLTASANIIMRKPQLLFQWRKCKHLIIDEISLISAEFFDHLDQVARVVRNNIKVPFGGIQLIISGDFFQLPPILPRN------ADKHYKFVFQSKVFEQAFKEIHFLTEVKRQSDQEFVAILSEIRMGRCSEATEKRLRTTANQNIERNGIIPTQLCTHKDDVESKNQSNLNSLPGEPEDFFAIDSIPGHRNTVDSVCMVPFKLTLKVGAQVMLLKNIDIAGGLANGSRGVVVGFEPAIKNRYPLVRFSSGKVYTMKPESFSVSISSSGQTLLIARSQIPLKLAYSISIHKSQGMTLDCVDLYLANTFECGQAYVALSRCKSLDSLRVMSWSNHCVKANHDVIKFFNKYQ 2027
+ ++F++EGKAT + ++N PPG L FL T+ K Q +KK + ++ SS + +SP+ ++ + KA + +P+ KKR ++ E+ K+ + P AK KL S T+ E +LS EQ +++ SG++VF+TGSAGTGK++LL++II L P T TASTG+AA LI G TLH FAGIG ++ + R P WRKCK LIIDEIS++ +FF+ ++ VAR +R N + PFGGIQLI+ GDF QLPP++ + A +F FQS +E + ++ L +V RQSD EFV IL+ +R+G +++ RL T+ Q IE NGI+ TQLC+H +D S N+S L +L G+ F A DS T+D P +L LKV AQVMLLKNI+I+ GL NG+RGVVV E + P+VRF + + Y K E + + +S I R Q+PLKLA++ SIHKSQG+TLDCV++ L+ FE GQAYVALSR KSL S+R++ + V AN V++F+ ++
Sbjct: 65 VHKKFMSEGKATFNFKAEKCTLYLSNAPPGTLMFFLRTIFVKMNGNEGGSQPDDATLQKKLREHLMSGKPSSFNDVSPVTTAEMILARKKAGLLSKGSVTTPSPQGAKKRRFEELKEE-----------KERGTMPAAK--------------KLYASTTD-----------ESLRLSEEQMEVLRACTSGKSVFFTGSAGTGKSFLLRRIISALPPDGTVATASTGVAACLIGGTTLHAFAGIGGGDATMQRCLELASR-PANAQTWRKCKRLIIDEISMVDGQFFEKIEAVARHIRRNDR-PFGGIQLILCGDFLQLPPVIKGDFGAAPTATPQQRFCFQSSAWETCIQCVYELKQVHRQSDPEFVKILNHLRIGHVNDSITSRLAATSKQKIEGNGILATQLCSHTNDANSINESKLENLDGDKILFKADDSDASMTRTLDQQIQAPSQLYLKVNAQVMLLKNINISNGLVNGARGVVVRMEKDL----PVVRFKNNQEYVCKHERWIIKTASGNH---ITRRQVPLKLAWAFSIHKSQGLTLDCVEMSLSKVFEAGQAYVALSRAKSLQSIRILDFDAKQVWANPHVLQFYKGFR 618
BLAST of ATP-dependent DNA helicase PIF1 vs. Ensembl Zebrafish
Match: pif1 (PIF1 5'-to-3' DNA helicase homolog (S. cerevisiae) [Source:NCBI gene;Acc:326729]) HSP 1 Score: 419.468 bits (1077), Expect = 1.203e-137 Identity = 254/600 (42.33%), Postives = 355/600 (59.17%), Query Frame = 3
Query: 243 YPLVDMQIFERFVTEGKATIKLPKSSVQIMINNCPPGDLKLFLSTLKYKTKIAVNTVQXXXXXXXXXXXXPRSISSLDTISPIRKSDKFKLQNKALENHFPDINPITPEKKRPLRDINEDFNTPPNKKLKIKQYTSTPNAKTSIKEIDNIFDSSEKLKKSATEFDVSLNDSGLTEYYQLSNEQRKIIECVRSGENVFYTGSAGTGKTYLLKKIIGTLVPQVXXXXXXXXXXXXLIDGCTLHNFAGIGLKMESLTASANIIMRKPQLLFQWRKCKHLIIDEISLISAEFFDHLDQVARVVRNNIKVPFGGIQLIISGDFFQLPPILPRNADKHYKFVFQSKVFEQAFKEIHFLTEVKRQSDQEFVAILSEIRMGRCSEATEKRLRTTANQNIERNGIIPTQLCTHKDDVESKNQSNLNSLPGEPEDFFAIDSIPGHRNTVDSVCMVPFKLTLKVGAQVMLLKNIDIAGGLANGSRGVVVGFEPAIKNRYPLVRFSSGKVYTMKPESFSVSISSSGQTLLIARSQIPLKLAYSISIHKSQGMTLDCVDLYLANTFECGQAYVALSRCKSLDSLRVMSWSNHCVKANHDVIKFFNKYQTDDIL 2042
+ L D Q+F RF +GK T+K + Q+++++CPP LK+FL TL K + A + + L ++ L +PR+ +T+SP++ D KA E P+ P R + E P NK L ++ P + + + KK V+ +LS EQ ++ V SG+NVF+TGSAGTGK++LLK+I+G+L P+ T TASTG+AA I G TLH+FAGIG L + R P +L W CKHLIIDEIS++ AEFFD L+ +AR +R + + PFGGIQLI+ GDF QLPP+ + F FQS+ + + L EV+RQ+D+ F+++L +R+GR +E +L +AN IER+GI+ T+LCTHKDDVE N++ L LPG + A+DS P T+D+ V L LKVGAQVML KN+D+ GL NG+RGVVV F+P + P VRF G V MK E + L ++R Q+PLKLA++ISIHKSQGMTLDCV++ LA FE GQAYVALSR +SL+ LRVM + V+AN DV+ F+ + + + +L
Sbjct: 59 FMLRDFQLFTRFAKDGKCTVKFIPENTQVLVSDCPPDRLKMFLKTLSIKHQ-ASQSSKPLSDREKLRAGLPRA---FETVSPLQLKDV----EKANELRSRVNAPMQP------RCLAE---RPANKVLGDRKQVKRPRPDCDASPVKALHPN----KKPVLVLPVA---------QKLSKEQTAVLNAVLSGKNVFFTGSAGTGKSFLLKRIVGSLPPKSTYATASTGVAACHIGGTTLHSFAGIGSGSAPLEQCIELAQR-PGVLRHWTSCKHLIIDEISMVEAEFFDKLEAIARSIRRSTE-PFGGIQLIVCGDFLQLPPVT--KGKEKANFCFQSRSWRKCIHMNMELMEVRRQTDKTFISLLQAVRVGRVTEEVTAQLLKSANHCIERDGILATRLCTHKDDVELTNENKLKQLPGVVRMYEAVDSDPMLVQTIDAQSPVSRLLQLKVGAQVMLTKNLDVQRGLVNGARGVVVDFQPGNQG-LPRVRFLCGAVEVMKRERWMFKAPGG---LYLSRQQLPLKLAWAISIHKSQGMTLDCVEISLARVFESGQAYVALSRARSLEGLRVMDFDPRVVQANQDVLIFYKRLRKERLL 620
BLAST of ATP-dependent DNA helicase PIF1 vs. Ensembl Zebrafish
Match: pif1 (PIF1 5'-to-3' DNA helicase homolog (S. cerevisiae) [Source:NCBI gene;Acc:326729]) HSP 1 Score: 419.083 bits (1076), Expect = 2.018e-137 Identity = 254/600 (42.33%), Postives = 355/600 (59.17%), Query Frame = 3
Query: 243 YPLVDMQIFERFVTEGKATIKLPKSSVQIMINNCPPGDLKLFLSTLKYKTKIAVNTVQXXXXXXXXXXXXPRSISSLDTISPIRKSDKFKLQNKALENHFPDINPITPEKKRPLRDINEDFNTPPNKKLKIKQYTSTPNAKTSIKEIDNIFDSSEKLKKSATEFDVSLNDSGLTEYYQLSNEQRKIIECVRSGENVFYTGSAGTGKTYLLKKIIGTLVPQVXXXXXXXXXXXXLIDGCTLHNFAGIGLKMESLTASANIIMRKPQLLFQWRKCKHLIIDEISLISAEFFDHLDQVARVVRNNIKVPFGGIQLIISGDFFQLPPILPRNADKHYKFVFQSKVFEQAFKEIHFLTEVKRQSDQEFVAILSEIRMGRCSEATEKRLRTTANQNIERNGIIPTQLCTHKDDVESKNQSNLNSLPGEPEDFFAIDSIPGHRNTVDSVCMVPFKLTLKVGAQVMLLKNIDIAGGLANGSRGVVVGFEPAIKNRYPLVRFSSGKVYTMKPESFSVSISSSGQTLLIARSQIPLKLAYSISIHKSQGMTLDCVDLYLANTFECGQAYVALSRCKSLDSLRVMSWSNHCVKANHDVIKFFNKYQTDDIL 2042
+ L D Q+F RF +GK T+K + Q+++++CPP LK+FL TL K + A + + L ++ L +PR+ +T+SP++ D KA E P+ P R + E P NK L ++ P + + + KK V+ +LS EQ ++ V SG+NVF+TGSAGTGK++LLK+I+G+L P+ T TASTG+AA I G TLH+FAGIG L + R P +L W CKHLIIDEIS++ AEFFD L+ +AR +R + + PFGGIQLI+ GDF QLPP+ + F FQS+ + + L EV+RQ+D+ F+++L +R+GR +E +L +AN IER+GI+ T+LCTHKDDVE N++ L LPG + A+DS P T+D+ V L LKVGAQVML KN+D+ GL NG+RGVVV F+P + P VRF G V MK E + L ++R Q+PLKLA++ISIHKSQGMTLDCV++ LA FE GQAYVALSR +SL+ LRVM + V+AN DV+ F+ + + + +L
Sbjct: 59 FMLRDFQLFTRFAKDGKCTVKFIPENTQVLVSDCPPDRLKMFLKTLSIKHQ-ASQSSKPLSDREKLRAGLPRA---FETVSPLQLKDV----EKANELRSRVNAPMQP------RCLAE---RPANKVLGDRKQVKRPRPDCDASPVKALHPN----KKPVLVLPVA---------QKLSKEQTAVLNAVLSGKNVFFTGSAGTGKSFLLKRIVGSLPPKSTYATASTGVAACHIGGTTLHSFAGIGSGSAPLEQCIELAQR-PGVLRHWTSCKHLIIDEISMVEAEFFDKLEAIARSIRRSTE-PFGGIQLIVCGDFLQLPPVT--KGKEKANFCFQSRSWRKCIHMNMELMEVRRQTDKTFISLLQAVRVGRVTEEVTAQLLKSANHCIERDGILATRLCTHKDDVELTNENKLKQLPGVVRMYEAVDSDPMLVQTIDAQSPVSRLLQLKVGAQVMLTKNLDVQRGLVNGARGVVVDFQPGNQG-LPRVRFLCGAVEVMKRERWMFKAPGG---LYLSRQQLPLKLAWAISIHKSQGMTLDCVEISLARVFESGQAYVALSRARSLEGLRVMDFDPRVVQANQDVLIFYKRLRKERLL 620
BLAST of ATP-dependent DNA helicase PIF1 vs. Ensembl Zebrafish
Match: BX927253.3 (pep chromosome:GRCz11:4:43367796:43374443:-1 gene:ENSDARG00000114317.1 transcript:ENSDART00000191022.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:BX927253.3) HSP 1 Score: 130.568 bits (327), Expect = 1.404e-30 Identity = 129/465 (27.74%), Postives = 209/465 (44.95%), Query Frame = 3
Query: 831 FYTGSAGTGKTYLLK-------KIIGTLVPQVXXXXXXXXXXXXL----------IDGCTLHN-----------FAGIGLKMESLTASANIIMRKPQLLFQWRKCKHLIIDEISLISAEFFDHLDQVARVVRNNIKVPFGGIQLIISGDFFQLPPILPRNADKHYKFVFQSKVFEQAFKEIHF----LTEVKRQ-SDQEFVAILSEIRMGRCSEATEKRLRTTANQNIERNGIIPTQ----LCTHKDDVESKNQSNLNSLPGE-----PEDFFAIDSIPGHRNTVDSVCMVPFKL----TLKVGAQVMLLKNIDIAGGLANGSRGVVVGFEPAIKNRYPLVRF--------SSGKVYTMK---PESFSVSISSSGQTLL--IARSQIPLKLAYSISIHKSQGMTLDCVDLYLANTFECGQAYVALSRCKSLDSLRVMSWSNHCVKANHDVIKFFNKYQTDDILGL 2048
F +G AG GK++++K KI+ L PQ+ + L I G TLH+ + G+G ++ + A + R + LIIDEIS+IS +FF +++ + +R + K PFGGI +I+ GDF+QLPP P A V++ V + F + HF LTE+ RQ D F +L+ +R+ R S+A ++ R+ Q ++ P T+K+ V S N +N+L + ED+ G + V L + VG ++M+++N+D+ GL NG G + ++ V ++G+ Y + E V I S + L R Q P+KLAY+ + HK QGMT+ + L FE G AYVALSR SL L + + + A+ ++ + + + G+
Sbjct: 1536 FVSGGAGCGKSHVIKCVYTEATKILRQL-PQLREDGDLSIPTVLLSAFTGTAAFNISGKTLHSILKLPKNLKPPYQGLGNSLDDVRA-------------ELRHVEILIIDEISMISKDFFAYINWRFQQIRGS-KKPFGGISVIVVGDFYQLPP--PGKAKP--LCVYEEDVLD--FWKDHFQIVTLTEIMRQKEDLSFAQLLNRLRVKRKSDALKEEDRSLLLQAVKNPQDCPRDALHIFATNKE-VHSHNCETVNALHADIVTIDAEDYRKDPRTGGMKRQTKPVMGKKDNLLDTIQVAVGVRIMVIRNLDVEDGLVNGCFGKIGNIVTKTRDGIAFVHMLGIQPDNPNAGQKYRRRLQGEEDTLVYIERSEEPLKKGTVRRQFPIKLAYACTAHKVQGMTMQSAVVSLKKIFEPGMAYVALSRTTSLGGLYIRDYDEKKIYADPEITTSLERMKMATLEGI 1978
BLAST of ATP-dependent DNA helicase PIF1 vs. Ensembl Zebrafish
Match: BX537305.1 (pep chromosome:GRCz11:3:42497814:42504515:1 gene:ENSDARG00000113834.1 transcript:ENSDART00000184653.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:BX537305.1) HSP 1 Score: 130.183 bits (326), Expect = 1.896e-30 Identity = 128/464 (27.59%), Postives = 209/464 (45.04%), Query Frame = 3
Query: 831 FYTGSAGTGKTYLLK-------KIIGTLVPQVXXXXXXXXXXXXL----------IDGCTLHN-----------FAGIGLKMESLTASANIIMRKPQLLFQWRKCKHLIIDEISLISAEFFDHLDQVARVVRNNIKVPFGGIQLIISGDFFQLPPILPRNADKHYKFVFQSKVFEQAFKEIHF----LTEVKRQ-SDQEFVAILSEIRMGRCSEATEKR-----LRTTAN-QNIERNG--IIPTQLCTHKDDVESKNQSNLNSLPGEPEDFFAIDSIPGHRNTVDSVCMVPFKL----TLKVGAQVMLLKNIDIAGGLANGSRGVVVGFEPAIKNRYPLVRF--------SSGKVYTMK---PESFSVSISSSGQTLL--IARSQIPLKLAYSISIHKSQGMTLDCVDLYLANTFECGQAYVALSRCKSLDSLRVMSWSNHCVKANHDVIKFFNKYQTDDILGL 2048
F +G AG GK++++K KI+ L PQ+ + L I G TLH+ + G+G ++ + A + R + LIIDEIS+IS +FF +++ + +R + K PFGGI +I+ GDF+QLPP P A V++ V + F + HF LTE+ RQ D F +L+ +R+ R S+A ++ L+ N Q+ R+ I T H + E+ N + + + + ED+ G + V L + VG ++M+++N+D+ GL NG G + ++ V ++G+ Y + E V I S + L R Q P+KLAY+ + HK QGMT+ + L FE G AYVALSR SL L + + + A+ ++ + + + G+
Sbjct: 1554 FVSGGAGCGKSHVIKCVYTEATKILRQL-PQLREDGDLSIPTVLLSAFTGTAAFNISGKTLHSILKLPKNLKPPYQGLGNSLDDVRA-------------ELRHVEILIIDEISMISKDFFAYINWRFQQIRGS-KKPFGGISVIVVGDFYQLPP--PGKAKP--LCVYEEDVLD--FWKDHFQIVTLTEIMRQKEDLSFAQLLNRLRVKRKSDALKEEDRALLLQAVKNPQDCPRDALHIFATNKEVHSHNCETVNALHADIVTIDAEDYRKDPRTGGMKRQTKPVMGKKDNLLDTIQVAVGVRIMVIRNLDVEDGLVNGCFGKIGNIVTKTRDGIAFVHMLGIQPDNPNAGQKYRRRLQGEEDTLVYIERSEEPLKKGTVRRQFPIKLAYACTAHKVQGMTMQSAVVSLKKIFEPGMAYVALSRTTSLGGLYIRDYDEKKIYADPEITTSLERMKMATLEGI 1996
BLAST of ATP-dependent DNA helicase PIF1 vs. Ensembl Zebrafish
Match: BX324227.2 (pep chromosome:GRCz11:15:29527420:29534022:-1 gene:ENSDARG00000109743.1 transcript:ENSDART00000182658.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:BX324227.2) HSP 1 Score: 129.028 bits (323), Expect = 4.651e-30 Identity = 129/472 (27.33%), Postives = 211/472 (44.70%), Query Frame = 3
Query: 831 FYTGSAGTGKTYLLK-------KIIGTLVPQVXXXXXXXXXXXXL----------IDGCTLHN-----------FAGIGLKMESLTASANIIMRKPQLLFQWRKCKHLIIDEISLISAEFFDHLDQVARVVRNNIKVPFGGIQLIISGDFFQLPPILPRNADKHYKFVFQSKVFEQAFKEIHF----LTEVKRQ-SDQEFVAILSEIRMGRCSEATEKRLRTTANQNIERNGIIPTQ----LCTHKDDVESKNQSNLNSLPGE-----PEDFFAIDSIPGHRNTVDSVCMVPFKL----TLKVGAQVMLLKNIDIAGGLANGSRGVVVGFEPAIKNRYPLVRF--------SSGKVYTMK---PESFSVSISSSGQTL--LIARSQIPLKLAYSISIHKSQGMTLDCVDLYLANTFECGQAYVALSRCKSLDSLRVMSWSNHCVKANHDVIKFFNKYQTDDILGLYEDF*YR 2069
F +G AG GK++++K KI+ L PQ+ + L I G TLH+ + G+G ++ + A R + LIIDEIS+IS +FF +++ + +R + K PFGGI +I+ GDF+QLPP P A V++ V + F + HF LTE+ RQ D F +L+ +R+ R S+A ++ R Q + P T+K+ V S N + +N+L + ED+ G + V L + VG ++M+++N+D+ GL NG G + ++ V ++G+ Y + + V I S + + R Q P+KLAY+ + HK QGMT+ + L FE G AYVALSR SL L + + + A+ ++ + +T + G+ F ++
Sbjct: 1521 FVSGGAGCGKSHVIKCVYTEATKILQQL-PQLREDGDLSIPTVILSAFTGTAAFNISGKTLHSILKLPRNLKPPYQGLGNSLDDVRAG-------------LRHVEILIIDEISMISKDFFAYINWRFQQIRGS-KKPFGGISVIVVGDFYQLPP--PGKAKP--LCVYEEDVLD--FWKDHFQIVTLTEIMRQKEDLAFAQLLNRLRVKRKSDALKEEDRALLLQAVRNPQDCPRDALHIFATNKE-VHSHNSNTVNALHADIITIHAEDYRKDPRTGGMKRQTKPVMGNKDDLLDTIQVAVGVRIMMIRNLDVEDGLVNGCFGNIGNIVTKTRDGIAFVHMLGIQPDNPNAGQKYRRRLQGDQDTLVYIERSEEPVKKRAVRRQFPIKLAYACTAHKVQGMTMQSAVVSLKRIFEPGMAYVALSRTTSLGGLYIRDFDEKKIYADPEITTSLERMKTATLEGIMPLFDWK 1970
BLAST of ATP-dependent DNA helicase PIF1 vs. Ensembl Xenopus
Match: ENSXETT00000049911.1 (ATP-dependent DNA helicase PIF1 [Source:NCBI gene;Acc:101730335]) HSP 1 Score: 415.231 bits (1066), Expect = 5.009e-135 Identity = 264/668 (39.52%), Postives = 389/668 (58.23%), Query Frame = 3
Query: 72 ITASVNLQSLGKDNNTVINTKQFKQALVESVREDDGQVMYVRITLISTSKKVTGQYGYPLVDMQIFERFVTEGKATIKLPKSSVQIMINNCPPGDLKLFLSTLKYKTKIAVNTVQXXXXXXXXXXXXPRSISSLDTISPIRKSD---KFKLQNKALENHFPDINPITPEKKRPLRDINEDFNTPPNKKLKIKQYTSTPNAKTSIKEIDNIFDSSEKLKKSATEFDVSLNDSGLTEYYQLSNEQRKIIECVRSGENVFYTGSAGTGKTYLLKKIIGTLVPQVXXXXXXXXXXXXLIDGCTLHNFAGIGLKMESLTASANIIMRKPQLLFQWRKCKHLIIDEISLISAEFFDHLDQVARVVRNNIKVPFGGIQLIISGDFFQLPPILPRNADKHYKFVFQSKVFEQAFKEIHFLTEVKRQSDQEFVAILSEIRMGRCSEATEKRLRTTANQNIERNGIIPTQLCTHKDDVESKNQSNLNSLPGEPEDFFAIDSIPGHRNTVDSVCMVPFKLTLKVGAQVMLLKNIDIAGGLANGSRGVVVGFEPAIKNRYPLVRFSSGKVYTMKPESFSVSISSSGQTLLIARSQIPLKLAYSISIHKSQGMTLDCVDLYLANTFECGQAYVALSRCKSLDSLRVMSWSNHCVKANHDVIKFFNKYQTDDIL--GLYEDF 2060
I +S+ ++ L T + K + ALV R + + + L + K+ Q + L +++F RF+ +GKA++ L +VQ++I+NCP L+ F+ TL K + A T + + ++ L +PR +TISP++K D +++ KA N TP K R L N N + +Q + + S+ I D K + + + + QLS EQ ++ V SG NVF+TGSAGTGK+YLLK+I+G L P+ T TASTG+AA I G TLH FAGIG S+ + ++P + W CKHLIIDEIS++ EFFD L+ VAR VR + PFGGIQLI+ GDF QLPP+ A KF FQ++ + + LTEV+RQ+D+ F+++L IR+GRC++ ++L T++ +ER+GI+ T+LCTHKDDVE N+ L LPGE + A+DS P T+++ C V ++ LK GAQVML KN+D++ GL NG+RGVV FE KN P+VRF G +KP+ + + + ++R Q+PLKLA++ISIHKSQGM+LDCV++ L+ FE GQAYVALSR ++L+ LRVM + V+AN V++F+++ + + L EDF
Sbjct: 79 IQSSITIEYLNSSGQT-LKRKAIRNALVSLGRNE-----FRDLVLKVSDGKL--QQNFVLKQIKLFTRFIRDGKASVVLLPENVQLLISNCPSDKLQHFMKTLLIKHE-AGKTEKPVNERTRLLSGLPR---VFETISPVQKKDVEQANEMRAKA--------NSETPVKGRGLS------NKGVNGVNRCQQKRTRTESSNSL-----IADLRPSKKPTLS----------MPKQVQLSTEQSLVLNTVLSGRNVFFTGSAGTGKSYLLKRIVGALPPKSTYATASTGVAACHIGGTTLHAFAGIGTGKASIEQCIE-LAKRPGVRQHWTGCKHLIIDEISMVEGEFFDKLEAVARAVRGKDE-PFGGIQLIVCGDFLQLPPVT--QASSQTKFCFQARSWRKCIHLTMELTEVRRQTDKNFISLLQAIRLGRCTDDVSRKLLQTSSHKVERDGILATRLCTHKDDVEITNERRLQQLPGESHLYEALDSDPMLVKTINAQCPVNQQIQLKKGAQVMLAKNLDVSRGLVNGARGVVTKFEEGNKN-LPVVRFLCGVTEVIKPDRW---VFKGHGGIYLSRQQLPLKLAWAISIHKSQGMSLDCVEISLSRVFESGQAYVALSRARNLEGLRVMDFDPKVVRANPYVLQFYSQMRKERALRQTCLEDF 697
BLAST of ATP-dependent DNA helicase PIF1 vs. Ensembl Xenopus
Match: ENSXETT00000049940.1 (ATP-dependent DNA helicase PIF1 [Source:NCBI gene;Acc:101730335]) HSP 1 Score: 410.223 bits (1053), Expect = 3.338e-134 Identity = 262/675 (38.81%), Postives = 385/675 (57.04%), Query Frame = 3
Query: 30 LF*FIGIIFMSHS-SITASVNLQSLGKDNNTVINTKQFKQALVESVREDDGQVMYVRITLISTSKKVTGQYGYPLVDMQIFERFVTEGKATIKLPKSSVQIMINNCPPGDLKLFLSTLKYKTKIAVNTVQXXXXXXXXXXXXPRSISSLDTISPIRKSD---KFKLQNKALENHFPDINPITPEKKRPLRDINEDFNTPPNKKLKIKQYTSTPNAKTSIKEIDNIFDSSEKLKKSATEFDVSLNDSGLTEYYQLSNEQRKIIECVRSGENVFYTGSAGTGKTYLLKKIIGTLVPQVXXXXXXXXXXXXLIDGCTLHNFAGIGLKMESLTASANIIMRKPQLLFQWRKCKHLIIDEISLISAEFFDHLDQVARVVRNNIKVPFGGIQLIISGDFFQLPPILPRNADKHYKFVFQSKVFEQAFKEIHFLTEVKRQSDQEFVAILSEIRMGRCSEATEKRLRTTANQNIERNGIIPTQLCTHKDDVESKNQSNLNSLPGEPEDFFAIDSIPGHRNTVDSVCMVPFKLTLKVGAQVMLLKNIDIAGGLANGSRGVVVGFEPAIKNRYPLVRFSSGKVYTMKPESFSVSISSSGQTLLIARSQIPLKLAYSISIHKSQGMTLDCVDLYLANTFECGQAYVALSRCKSLDSLRVMSWSNHCVKANHDVIKFFNKYQTDDIL 2042
LF II++ S I +S+ ++ L T + K + ALV R + + + L + K+ Q + L +++F RF+ +GKA++ L +VQ++I+NCP L+ F+ TL K + A T + + ++ L +PR +TISP++K D +++ KA N TP K R L NK + + + P + + QLS EQ ++ V SG NVF+TGSAGTGK+YLLK+I+G L P+ T TASTG+AA I G TLH FAGIG S+ + ++P + W CKHLIIDEIS++ EFFD L+ VAR VR + PFGGIQLI+ GDF QLPP+ A KF FQ++ + + LTEV+RQ+D+ F+++L IR+GRC++ ++L T++ +ER+GI+ T+LCTHKDDVE N+ L LPGE + A+DS P T+++ C V ++ LK GAQVML KN+D++ GL NG+RGVV FE KN P+VRF G +KP+ + + + ++R Q+PLKLA++ISIHKSQGM+LDCV++ L+ FE GQAYVALSR ++L+ LRVM + V+AN V++F+++ + + L
Sbjct: 2 LFGISAIIWLLLSPEIQSSITIEYLNSSGQT-LKRKAIRNALVSLGRNE-----FRDLVLKVSDGKL--QQNFVLKQIKLFTRFIRDGKASVVLLPENVQLLISNCPSDKLQHFMKTLLIKHE-AGKTEKPVNERTRLLSGLPRV---FETISPVQKKDVEQANEMRAKA--------NSETPVKGRGL----------SNKGIADLRPSKKPTL-------------------------------SMPKQVQLSTEQSLVLNTVLSGRNVFFTGSAGTGKSYLLKRIVGALPPKSTYATASTGVAACHIGGTTLHAFAGIGTGKASIEQCIEL-AKRPGVRQHWTGCKHLIIDEISMVEGEFFDKLEAVARAVRGKDE-PFGGIQLIVCGDFLQLPPVT--QASSQTKFCFQARSWRKCIHLTMELTEVRRQTDKNFISLLQAIRLGRCTDDVSRKLLQTSSHKVERDGILATRLCTHKDDVEITNERRLQQLPGESHLYEALDSDPMLVKTINAQCPVNQQIQLKKGAQVMLAKNLDVSRGLVNGARGVVTKFEEGNKN-LPVVRFLCGVTEVIKPDRW---VFKGHGGIYLSRQQLPLKLAWAISIHKSQGMSLDCVEISLSRVFESGQAYVALSRARNLEGLRVMDFDPKVVRANPYVLQFYSQMRKERAL 607
BLAST of ATP-dependent DNA helicase PIF1 vs. Ensembl Xenopus
Match: ENSXETT00000049935.1 (ATP-dependent DNA helicase PIF1 [Source:NCBI gene;Acc:101730335]) HSP 1 Score: 384.03 bits (985), Expect = 2.044e-124 Identity = 203/422 (48.10%), Postives = 282/422 (66.82%), Query Frame = 3
Query: 777 LSNEQRKIIECVRSGENVFYTGSAGTGKTYLLKKIIGTLVPQVXXXXXXXXXXXXLIDGCTLHNFAGIGLKMESLTASANIIMRKPQLLFQWRKCKHLIIDEISLISAEFFDHLDQVARVVRNNIKVPFGGIQLIISGDFFQLPPILPRNADKHYKFVFQSKVFEQAFKEIHFLTEVKRQSDQEFVAILSEIRMGRCSEATEKRLRTTANQNIERNGIIPTQLCTHKDDVESKNQSNLNSLPGEPEDFFAIDSIPGHRNTVDSVCMVPFKLTLKVGAQVMLLKNIDIAGGLANGSRGVVVGFEPAIKNRYPLVRFSSGKVYTMKPESFSVSISSSGQTLLIARSQIPLKLAYSISIHKSQGMTLDCVDLYLANTFECGQAYVALSRCKSLDSLRVMSWSNHCVKANHDVIKFFNKYQTDDIL 2042
LS EQ ++ V SG NVF+TGSAGTGK+YLLK+I+G L P+ T TASTG+AA I G TLH FAGIG S+ + ++P + W CKHLIIDEIS++ EFFD L+ VAR VR + PFGGIQLI+ GDF QLPP+ A KF FQ++ + + LTEV+RQ+D+ F+++L IR+GRC++ ++L T++ +ER+GI+ T+LCTHKDDVE N+ L LPGE + A+DS P T+++ C V ++ LK GAQVML KN+D++ GL NG+RGVV FE KN P+VRF G +KP+ + + + ++R Q+PLKLA++ISIHKSQGM+LDCV++ L+ FE GQAYVALSR ++L+ LRVM + V+AN V++F+++ + + L
Sbjct: 169 LSTEQSLVLNTVLSGRNVFFTGSAGTGKSYLLKRIVGALPPKSTYATASTGVAACHIGGTTLHAFAGIGTGKASIEQCIEL-AKRPGVRQHWTGCKHLIIDEISMVEGEFFDKLEAVARAVRGKDE-PFGGIQLIVCGDFLQLPPVT--QASSQTKFCFQARSWRKCIHLTMELTEVRRQTDKNFISLLQAIRLGRCTDDVSRKLLQTSSHKVERDGILATRLCTHKDDVEITNERRLQQLPGESHLYEALDSDPMLVKTINAQCPVNQQIQLKKGAQVMLAKNLDVSRGLVNGARGVVTKFEEGNKN-LPVVRFLCGVTEVIKPDRW---VFKGHGGIYLSRQQLPLKLAWAISIHKSQGMSLDCVEISLSRVFESGQAYVALSRARNLEGLRVMDFDPKVVRANPYVLQFYSQMRKERAL 582 HSP 2 Score: 50.447 bits (119), Expect = 8.728e-6 Identity = 42/144 (29.17%), Postives = 76/144 (52.78%), Query Frame = 3
Query: 72 ITASVNLQSLGKDNNTVINTKQFKQALVESVREDDGQVMYVRITLISTSKKVTGQYGYPLVDMQIFERFVTEGKATIKLPKSSVQIMINNCPPGDLKLFLSTLKYKTKIAVNTVQXXXXXXXXXXXXPRSISSLDTISPIRKSD 503
I +S+ ++ L T + K + ALV R + + + L + K+ Q + L +++F RF+ +GKA++ L +VQ++I+NCP L+ F+ TL K + A T + + ++ L +PR +TISP++K D
Sbjct: 11 IQSSITIEYLNSSGQT-LKRKAIRNALVSLGRNE-----FRDLVLKVSDGKL--QQNFVLKQIKLFTRFIRDGKASVVLLPENVQLLISNCPSDKLQHFMKTLLIKHE-AGKTEKPVNERTRLLSGLPR---VFETISPVQKKD 142
BLAST of ATP-dependent DNA helicase PIF1 vs. Ensembl Xenopus
Match: decr1 (2,4-dienoyl-CoA reductase 1 [Source:Xenbase;Acc:XB-GENE-985109]) HSP 1 Score: 350.517 bits (898), Expect = 1.178e-111 Identity = 231/598 (38.63%), Postives = 341/598 (57.02%), Query Frame = 3
Query: 72 ITASVNLQSLGKDNNTVINTKQFKQALVESVREDDGQVMYVRITLISTSKKVTGQYGYPLVDMQIFERFVTEGKATIKLPKSSVQIMINNCPPGDLKLFLSTLKYKTKIAVNTVQXXXXXXXXXXXXPRSISSLDTISPIRKSD---KFKLQNKALENHFPDINPITPEKKRPLRDINEDFNTPPNKKLKIKQYTSTPNAKTSIKEIDNIFDSSEKLKKSATEFDVSLNDSGLTEYYQLSNEQRKIIECVRSGENVFYTGSAGTGKTYLLKKIIGTLVPQVXXXXXXXXXXXXLIDGCTLHNFAGIGLKMESLTASANIIMRKPQLLFQWRKCKHLIIDEISLISAEFFDHLDQVARVVRNNIKVPFGGIQLIISGDFFQLPPILPRNADKHYKFVFQSKVFEQAFKEIHFLTEVKRQSDQEFVAILSEIRMGRCSEATEKRLRTTANQNIERNGIIPTQLCTHKDDVESKNQSNLNSLPGEPEDFFAIDSIPGHRNTVDSVCMVPFKLTLKVGAQVMLLKNIDIAGGLANGSRGVVVGFEPAIKNRYPLVRFSSGKVYTMKPESFSVSISSSGQTLLIARSQIPLKLAYSISIHKSQ 1856
I +S+ ++ L T + K + ALV R + + + L + K+ Q + L +++F RF+ +GKA++ L +VQ++I+NCP L+ F+ TL K + A T + + ++ L +PR +TISP++K D +++ KA N TP K R L N N + +Q + + S+ I D K + + + + +LS EQ ++ V SG NVF+TGSAGTGK+YLLK+I+G L P+ T TASTG+AA I G TLH FAGIG S+ + ++P + W CKHLIIDEIS++ EFFD L+ VAR VR + PFGGIQLI+ GDF QLPP+ A KF FQ++ + + LTEV+RQ+D+ F+++L IR+GRC++ ++L T++ +ER+GI+ T+LCTHKDDVE N+ L LPGE + A+DS P T+++ C V ++ LK GAQVML KN+D++ GL NG+RGVV FE KN P+VRF G +KP+ + + + ++R Q+PLKLA++ISIHKSQ
Sbjct: 11 IQSSITIEYLNSSGQT-LKRKAIRNALVSLGRNE-----FRDLVLKVSDGKL--QQNFVLNQIKLFTRFIRDGKASVVLLPENVQLLISNCPSDKLQHFMKTLLIKHE-AGKTEKPVNERTRLLSGLPR---VFETISPVQKKDVEQANEMRAKA--------NSETPVKGRGLS------NKGVNGVNRCQQKRTRTESSNSL-----IADLRPSKKPTLS----------MPKQVRLSTEQSLVLNTVLSGRNVFFTGSAGTGKSYLLKRIVGALPPKSTYATASTGVAACHIGGTTLHAFAGIGTGKASIEQCIE-LAKRPGVRQHWTGCKHLIIDEISMVEGEFFDKLEAVARAVRGKDE-PFGGIQLIVCGDFLQLPPVT--QASSQTKFCFQARSWRKCIHLTMELTEVRRQTDKNFISLLQAIRLGRCTDDVSRKLLQTSSHKVERDGILATRLCTHKDDVEITNERRLQQLPGESHLYEALDSDPMLVKTINAQCPVNQQIQLKKGAQVMLAKNLDVSRGLVNGARGVVTKFEEGNKN-LPVVRFLCGVTEVIKPDRW---VFKGHGGIYLSRQQLPLKLAWAISIHKSQ 559
BLAST of ATP-dependent DNA helicase PIF1 vs. Ensembl Xenopus
Match: decr1 (2,4-dienoyl-CoA reductase 1 [Source:Xenbase;Acc:XB-GENE-985109]) HSP 1 Score: 350.132 bits (897), Expect = 2.984e-111 Identity = 231/600 (38.50%), Postives = 342/600 (57.00%), Query Frame = 3
Query: 72 ITASVNLQSLGKDNNTVINTKQFKQALVESVREDDGQVMYVRITLISTSKKVTGQYGYPLVDMQIFERFVTEGKATIKLPKSSVQIMINNCPPGDLKLFLSTLKYKTKIAVNTVQXXXXXXXXXXXXPRSISSLDTISPIRKSD---KFKLQNKALENHFPDINPITPEKKRPLRDINEDFNTPPNKKLKIKQYTSTPNAKTSIKEIDNIFDSSEKLKKSATEFDVSLNDSGLTEYYQLSNEQRKIIECVRSGENVFYTGSAGTGKTYLLKKIIGTLVPQVXXXXXXXXXXXXLIDGCTLHNFAGIGLKMESLTASANIIMRKPQLLFQWRKCKHLIIDEISLISAEFFDHLDQVARVVRNNIKVPFGGIQLIISGDFFQLPPILPRNADKHYKFVFQSKVFEQAFKEIHFLTEVKRQSDQEFVAILSEIRMGRCSEATEKRLRTTANQNIERNGIIPTQLCTHKDDVESKNQSNLNSLPGEPEDFFAIDSIPGHRNTVDSVCMVPFKLTLKVGAQVMLLKNIDIAGGLANGSRGVVVGFEPAIKNRYPLVRFSSGKVYTMKPESFSVSISSSGQTLLIARSQIPLKLAYSISIHKSQGM 1862
I +S+ ++ L T + K + ALV R + + + L + K+ Q + L +++F RF+ +GKA++ L +VQ++I+NCP L+ F+ TL K + A T + + ++ L +PR +TISP++K D +++ KA N TP K R L N N + +Q + + S+ I D K + + + + +LS EQ ++ V SG NVF+TGSAGTGK+YLLK+I+G L P+ T TASTG+AA I G TLH FAGIG S+ + ++P + W CKHLIIDEIS++ EFFD L+ VAR VR + PFGGIQLI+ GDF QLPP+ A KF FQ++ + + LTEV+RQ+D+ F+++L IR+GRC++ ++L T++ +ER+GI+ T+LCTHKDDVE N+ L LPGE + A+DS P T+++ C V ++ LK GAQVML KN+D++ GL NG+RGVV FE KN P+VRF G +KP+ + + + ++R Q+PLKLA++ISIHKSQ +
Sbjct: 11 IQSSITIEYLNSSGQT-LKRKAIRNALVSLGRNE-----FRDLVLKVSDGKL--QQNFVLNQIKLFTRFIRDGKASVVLLPENVQLLISNCPSDKLQHFMKTLLIKHE-AGKTEKPVNERTRLLSGLPR---VFETISPVQKKDVEQANEMRAKA--------NSETPVKGRGLS------NKGVNGVNRCQQKRTRTESSNSL-----IADLRPSKKPTLS----------MPKQVRLSTEQSLVLNTVLSGRNVFFTGSAGTGKSYLLKRIVGALPPKSTYATASTGVAACHIGGTTLHAFAGIGTGKASIEQCIE-LAKRPGVRQHWTGCKHLIIDEISMVEGEFFDKLEAVARAVRGKDE-PFGGIQLIVCGDFLQLPPVT--QASSQTKFCFQARSWRKCIHLTMELTEVRRQTDKNFISLLQAIRLGRCTDDVSRKLLQTSSHKVERDGILATRLCTHKDDVEITNERRLQQLPGESHLYEALDSDPMLVKTINAQCPVNQQIQLKKGAQVMLAKNLDVSRGLVNGARGVVTKFEEGNKN-LPVVRFLCGVTEVIKPDRW---VFKGHGGIYLSRQQLPLKLAWAISIHKSQAI 561
BLAST of ATP-dependent DNA helicase PIF1 vs. Ensembl Mouse
Match: Pif1 (PIF1 5'-to-3' DNA helicase [Source:MGI Symbol;Acc:MGI:2143057]) HSP 1 Score: 382.874 bits (982), Expect = 2.810e-123 Identity = 240/610 (39.34%), Postives = 345/610 (56.56%), Query Frame = 3
Query: 243 YPLVDMQIFERFVTEGKATIKLP------KSSVQIMINNCPPGDLKLFLSTLKYKTKIAVNTVQXXXXXXXXXXXXPRSISSLDTISP--IRKSDKFKLQNKALENHFPDINPITPEKKRPLRDINEDFNTPPNKKLKIKQYTSTPNAKTSIKEIDNIFDSSEKLKKSATEFDVSLNDSGLTEYYQLSNEQRKIIECVRSGENVFYTGSAGTGKTYLLKKIIGTLVPQVXXXXXXXXXXXXLIDGCTLHNFAGIGLKMESLTASANIIMRKPQLLFQWRKCKHLIIDEISLISAEFFDHLDQVARVVRNNIKVPFGGIQLIISGDFFQLPPILPRNADKHYKFVFQSKVFEQAFKEIHFLTEVKRQSDQEFVAILSEIRMGRCSEATEKRLRTTANQNIERNGIIPTQLCTHKDDVESKNQSNLNSLPGEPEDFFAIDSIPGHRNTVDSVCMVPFKLTLKVGAQVMLLKNIDIAGGLANGSRGVVVGFEPAIKNRYPLVRFSSGKVYTMKPESFSVSISSSGQTLLIARSQIPLKLAYSISIHKSQGMTLDCVDLYLANTFECGQAYVALSRCKSLDSLRVMSWSNHCVKANHDVIKFFNKYQTDDILGL 2048
+PL +++F RF G++T++LP SVQ+++++CPP L+ FL TL+ K +A + L PR ++ + P ++++ K + ALE + T + PL P KKL++ P+ K +LS EQ ++ V G+++F+TGSAGTGK+YLLK I+G+L P T TASTG+AA I G TLH FAGIG L A + +P + W C+ L+IDEIS++ A+FFD L+ VAR VR K PFGGIQLII GDF QLPP+ + +F FQ+K + + I LTEV RQ+DQ F+++L +R+GRCS+ ++LR TA + R+GI+ T+LCTH+DDV N+ L +LPG+ F AIDS P T+D+ C V L LK+GAQVML+KN+ ++ GL NG+RGVVVGFE + P VRF G ++ + ++V + + GQ L +R Q+PL+LA++ISIHKSQGM+LDCV++ L F GQAYVALSR +SL LRV+ + V+ + V+ F+ + L L
Sbjct: 82 FPLRAVRLFTRFAATGRSTLRLPTDGVPGAGSVQLLLSDCPPERLRRFLRTLRLKLAVAPGP-GPASARAQLLGPRPRDFVTISPVQPEELQRAAATKAPDSALEKRPMESQTSTEAPRWPL----------PVKKLRM------PSTKP-----------------------------------KLSEEQAAVLRMVLKGQSIFFTGSAGTGKSYLLKHILGSLPPTGTVATASTGVAACHIGGTTLHAFAGIGSGQAPL-AQCMALANRPGVRQGWLNCQRLVIDEISMVEADFFDKLEAVARAVRQQ-KKPFGGIQLIICGDFLQLPPVT--KGSQQPQFCFQAKSWRRCVPVILELTEVWRQADQTFISLLQAVRLGRCSDEVTRQLRATAAHKVGRDGIVATRLCTHQDDVALTNEKWLKALPGDVHSFEAIDSDPELSRTLDAQCPVSRVLQLKLGAQVMLVKNLAVSRGLVNGARGVVVGFESEGRG-LPRVRFLCGITEVIRTDRWTVQV-TGGQYL--SRQQLPLQLAWAISIHKSQGMSLDCVEISLGRVFASGQAYVALSRARSLQGLRVLDFDPTVVRCDSRVLHFYATLRQGRGLSL 631
BLAST of ATP-dependent DNA helicase PIF1 vs. Ensembl Mouse
Match: Pif1 (PIF1 5'-to-3' DNA helicase [Source:MGI Symbol;Acc:MGI:2143057]) HSP 1 Score: 382.874 bits (982), Expect = 2.810e-123 Identity = 240/610 (39.34%), Postives = 345/610 (56.56%), Query Frame = 3
Query: 243 YPLVDMQIFERFVTEGKATIKLP------KSSVQIMINNCPPGDLKLFLSTLKYKTKIAVNTVQXXXXXXXXXXXXPRSISSLDTISP--IRKSDKFKLQNKALENHFPDINPITPEKKRPLRDINEDFNTPPNKKLKIKQYTSTPNAKTSIKEIDNIFDSSEKLKKSATEFDVSLNDSGLTEYYQLSNEQRKIIECVRSGENVFYTGSAGTGKTYLLKKIIGTLVPQVXXXXXXXXXXXXLIDGCTLHNFAGIGLKMESLTASANIIMRKPQLLFQWRKCKHLIIDEISLISAEFFDHLDQVARVVRNNIKVPFGGIQLIISGDFFQLPPILPRNADKHYKFVFQSKVFEQAFKEIHFLTEVKRQSDQEFVAILSEIRMGRCSEATEKRLRTTANQNIERNGIIPTQLCTHKDDVESKNQSNLNSLPGEPEDFFAIDSIPGHRNTVDSVCMVPFKLTLKVGAQVMLLKNIDIAGGLANGSRGVVVGFEPAIKNRYPLVRFSSGKVYTMKPESFSVSISSSGQTLLIARSQIPLKLAYSISIHKSQGMTLDCVDLYLANTFECGQAYVALSRCKSLDSLRVMSWSNHCVKANHDVIKFFNKYQTDDILGL 2048
+PL +++F RF G++T++LP SVQ+++++CPP L+ FL TL+ K +A + L PR ++ + P ++++ K + ALE + T + PL P KKL++ P+ K +LS EQ ++ V G+++F+TGSAGTGK+YLLK I+G+L P T TASTG+AA I G TLH FAGIG L A + +P + W C+ L+IDEIS++ A+FFD L+ VAR VR K PFGGIQLII GDF QLPP+ + +F FQ+K + + I LTEV RQ+DQ F+++L +R+GRCS+ ++LR TA + R+GI+ T+LCTH+DDV N+ L +LPG+ F AIDS P T+D+ C V L LK+GAQVML+KN+ ++ GL NG+RGVVVGFE + P VRF G ++ + ++V + + GQ L +R Q+PL+LA++ISIHKSQGM+LDCV++ L F GQAYVALSR +SL LRV+ + V+ + V+ F+ + L L
Sbjct: 82 FPLRAVRLFTRFAATGRSTLRLPTDGVPGAGSVQLLLSDCPPERLRRFLRTLRLKLAVAPGP-GPASARAQLLGPRPRDFVTISPVQPEELQRAAATKAPDSALEKRPMESQTSTEAPRWPL----------PVKKLRM------PSTKP-----------------------------------KLSEEQAAVLRMVLKGQSIFFTGSAGTGKSYLLKHILGSLPPTGTVATASTGVAACHIGGTTLHAFAGIGSGQAPL-AQCMALANRPGVRQGWLNCQRLVIDEISMVEADFFDKLEAVARAVRQQ-KKPFGGIQLIICGDFLQLPPVT--KGSQQPQFCFQAKSWRRCVPVILELTEVWRQADQTFISLLQAVRLGRCSDEVTRQLRATAAHKVGRDGIVATRLCTHQDDVALTNEKWLKALPGDVHSFEAIDSDPELSRTLDAQCPVSRVLQLKLGAQVMLVKNLAVSRGLVNGARGVVVGFESEGRG-LPRVRFLCGITEVIRTDRWTVQV-TGGQYL--SRQQLPLQLAWAISIHKSQGMSLDCVEISLGRVFASGQAYVALSRARSLQGLRVLDFDPTVVRCDSRVLHFYATLRQGRGLSL 631
BLAST of ATP-dependent DNA helicase PIF1 vs. Ensembl Mouse
Match: Pif1 (PIF1 5'-to-3' DNA helicase [Source:MGI Symbol;Acc:MGI:2143057]) HSP 1 Score: 382.874 bits (982), Expect = 2.810e-123 Identity = 240/610 (39.34%), Postives = 345/610 (56.56%), Query Frame = 3
Query: 243 YPLVDMQIFERFVTEGKATIKLP------KSSVQIMINNCPPGDLKLFLSTLKYKTKIAVNTVQXXXXXXXXXXXXPRSISSLDTISP--IRKSDKFKLQNKALENHFPDINPITPEKKRPLRDINEDFNTPPNKKLKIKQYTSTPNAKTSIKEIDNIFDSSEKLKKSATEFDVSLNDSGLTEYYQLSNEQRKIIECVRSGENVFYTGSAGTGKTYLLKKIIGTLVPQVXXXXXXXXXXXXLIDGCTLHNFAGIGLKMESLTASANIIMRKPQLLFQWRKCKHLIIDEISLISAEFFDHLDQVARVVRNNIKVPFGGIQLIISGDFFQLPPILPRNADKHYKFVFQSKVFEQAFKEIHFLTEVKRQSDQEFVAILSEIRMGRCSEATEKRLRTTANQNIERNGIIPTQLCTHKDDVESKNQSNLNSLPGEPEDFFAIDSIPGHRNTVDSVCMVPFKLTLKVGAQVMLLKNIDIAGGLANGSRGVVVGFEPAIKNRYPLVRFSSGKVYTMKPESFSVSISSSGQTLLIARSQIPLKLAYSISIHKSQGMTLDCVDLYLANTFECGQAYVALSRCKSLDSLRVMSWSNHCVKANHDVIKFFNKYQTDDILGL 2048
+PL +++F RF G++T++LP SVQ+++++CPP L+ FL TL+ K +A + L PR ++ + P ++++ K + ALE + T + PL P KKL++ P+ K +LS EQ ++ V G+++F+TGSAGTGK+YLLK I+G+L P T TASTG+AA I G TLH FAGIG L A + +P + W C+ L+IDEIS++ A+FFD L+ VAR VR K PFGGIQLII GDF QLPP+ + +F FQ+K + + I LTEV RQ+DQ F+++L +R+GRCS+ ++LR TA + R+GI+ T+LCTH+DDV N+ L +LPG+ F AIDS P T+D+ C V L LK+GAQVML+KN+ ++ GL NG+RGVVVGFE + P VRF G ++ + ++V + + GQ L +R Q+PL+LA++ISIHKSQGM+LDCV++ L F GQAYVALSR +SL LRV+ + V+ + V+ F+ + L L
Sbjct: 82 FPLRAVRLFTRFAATGRSTLRLPTDGVPGAGSVQLLLSDCPPERLRRFLRTLRLKLAVAPGP-GPASARAQLLGPRPRDFVTISPVQPEELQRAAATKAPDSALEKRPMESQTSTEAPRWPL----------PVKKLRM------PSTKP-----------------------------------KLSEEQAAVLRMVLKGQSIFFTGSAGTGKSYLLKHILGSLPPTGTVATASTGVAACHIGGTTLHAFAGIGSGQAPL-AQCMALANRPGVRQGWLNCQRLVIDEISMVEADFFDKLEAVARAVRQQ-KKPFGGIQLIICGDFLQLPPVT--KGSQQPQFCFQAKSWRRCVPVILELTEVWRQADQTFISLLQAVRLGRCSDEVTRQLRATAAHKVGRDGIVATRLCTHQDDVALTNEKWLKALPGDVHSFEAIDSDPELSRTLDAQCPVSRVLQLKLGAQVMLVKNLAVSRGLVNGARGVVVGFESEGRG-LPRVRFLCGITEVIRTDRWTVQV-TGGQYL--SRQQLPLQLAWAISIHKSQGMSLDCVEISLGRVFASGQAYVALSRARSLQGLRVLDFDPTVVRCDSRVLHFYATLRQGRGLSL 631
BLAST of ATP-dependent DNA helicase PIF1 vs. Ensembl Mouse
Match: Pif1 (PIF1 5'-to-3' DNA helicase [Source:MGI Symbol;Acc:MGI:2143057]) HSP 1 Score: 189.504 bits (480), Expect = 5.376e-53 Identity = 137/384 (35.68%), Postives = 200/384 (52.08%), Query Frame = 3
Query: 243 YPLVDMQIFERFVTEGKATIKLP------KSSVQIMINNCPPGDLKLFLSTLKYKTKIAVNTVQXXXXXXXXXXXXPRSISSLDTISP--IRKSDKFKLQNKALENHFPDINPITPEKKRPLRDINEDFNTPPNKKLKIKQYTSTPNAKTSIKEIDNIFDSSEKLKKSATEFDVSLNDSGLTEYYQLSNEQRKIIECVRSGENVFYTGSAGTGKTYLLKKIIGTLVPQVXXXXXXXXXXXXLIDGCTLHNFAGIGLKMESLTASANIIMRKPQLLFQWRKCKHLIIDEISLISAEFFDHLDQVARVVRNNIKVPFGGIQLIISGDFFQLPPILPRNADKHYKFVFQSKVFEQAFKEIHFLTEVKRQSDQEFVAILSEIRMGRCS 1370
+PL +++F RF G++T++LP SVQ+++++CPP L+ FL TL+ K +A + L PR ++ + P ++++ K + ALE KRP+ + T P +K KL+ +T+ +LS EQ ++ V G+++F+TGSAGTGK+YLLK I+G+L P T TASTG+AA I G TLH FAGIG L A + +P + W C+ L+IDEIS++ A+FFD L+ VAR VR K PFGGIQLII GDF QLPP+ + +F FQ+K + + I LTEV RQ+DQ F+++L +R+GR S
Sbjct: 82 FPLRAVRLFTRFAATGRSTLRLPTDGVPGAGSVQLLLSDCPPERLRRFLRTLRLKLAVAPGP-GPASARAQLLGPRPRDFVTISPVQPEELQRAAATKAPDSALE-------------KRPMES---------------QTSTEAPRWPLPVK----------KLRMPSTK-------------PKLSEEQAAVLRMVLKGQSIFFTGSAGTGKSYLLKHILGSLPPTGTVATASTGVAACHIGGTTLHAFAGIGSGQAPL-AQCMALANRPGVRQGWLNCQRLVIDEISMVEADFFDKLEAVARAVRQQ-KKPFGGIQLIICGDFLQLPPVT--KGSQQPQFCFQAKSWRRCVPVILELTEVWRQADQTFISLLQAVRLGRGS 409
BLAST of ATP-dependent DNA helicase PIF1 vs. UniProt/SwissProt
Match: sp|Q7ZV90|PIF1_DANRE (ATP-dependent DNA helicase PIF1 OS=Danio rerio OX=7955 GN=pif1 PE=2 SV=1) HSP 1 Score: 418.698 bits (1075), Expect = 1.730e-136 Identity = 254/600 (42.33%), Postives = 355/600 (59.17%), Query Frame = 3
Query: 243 YPLVDMQIFERFVTEGKATIKLPKSSVQIMINNCPPGDLKLFLSTLKYKTKIAVNTVQXXXXXXXXXXXXPRSISSLDTISPIRKSDKFKLQNKALENHFPDINPITPEKKRPLRDINEDFNTPPNKKLKIKQYTSTPNAKTSIKEIDNIFDSSEKLKKSATEFDVSLNDSGLTEYYQLSNEQRKIIECVRSGENVFYTGSAGTGKTYLLKKIIGTLVPQVXXXXXXXXXXXXLIDGCTLHNFAGIGLKMESLTASANIIMRKPQLLFQWRKCKHLIIDEISLISAEFFDHLDQVARVVRNNIKVPFGGIQLIISGDFFQLPPILPRNADKHYKFVFQSKVFEQAFKEIHFLTEVKRQSDQEFVAILSEIRMGRCSEATEKRLRTTANQNIERNGIIPTQLCTHKDDVESKNQSNLNSLPGEPEDFFAIDSIPGHRNTVDSVCMVPFKLTLKVGAQVMLLKNIDIAGGLANGSRGVVVGFEPAIKNRYPLVRFSSGKVYTMKPESFSVSISSSGQTLLIARSQIPLKLAYSISIHKSQGMTLDCVDLYLANTFECGQAYVALSRCKSLDSLRVMSWSNHCVKANHDVIKFFNKYQTDDIL 2042
+ L D Q+F RF +GK T+K + Q+++++CPP LK+FL TL K + A + + L ++ L +PR + +T+SP++ D KA E P+ P R + E P NK L ++ P + + + KK V+ +LS EQ ++ V SG+NVF+TGSAGTGK++LLK+I+G+L P+ T TASTG+AA I G TLH+FAGIG L + R P +L W CKHLIIDEIS++ AEFFD L+ +AR +R + + PFGGIQLI+ GDF QLPP+ + F FQS+ + + L EV+RQ+D+ F+++L +R+GR +E +L +AN IER+GI+ T+LCTHKDDVE N++ L LPG + A+DS P T+D+ V L LKVGAQVML KN+D+ GL NG+RGVVV F+P + P VRF G V MK E + L ++R Q+PLKLA++ISIHKSQGMTLDCV++ LA FE GQAYVALSR +SL+ LRVM + V+AN DV+ F+ + + + +L
Sbjct: 59 FMLRDFQLFTRFAKDGKCTVKFVPENTQVLVSDCPPDRLKMFLKTLSIKHQ-ASQSSKPLSDREKLRAGLPR---AFETVSPLQLKDV----EKANELRSRVNAPMQP------RCLAE---RPVNKVLGDRKQVKRPRPDCDASPVKALHPN----KKPVLVLPVA---------QKLSKEQTAVLNAVLSGKNVFFTGSAGTGKSFLLKRIVGSLPPKSTYATASTGVAACHIGGTTLHSFAGIGSGSAPLEQCIELAQR-PGVLRHWTSCKHLIIDEISMVEAEFFDKLEAIARSIRRSTE-PFGGIQLIVCGDFLQLPPVT--KGKEKANFCFQSRSWRKCIHMNMELMEVRRQTDKTFISLLQAVRVGRVTEEVTAQLLKSANHCIERDGILATRLCTHKDDVELTNENKLKQLPGVVRMYEAVDSDPMLVQTIDAQSPVSRLLQLKVGAQVMLTKNLDVQRGLVNGARGVVVDFQPGNQG-LPRVRFLCGAVEVMKRERWMFKAPGG---LYLSRQQLPLKLAWAISIHKSQGMTLDCVEISLARVFESGQAYVALSRARSLEGLRVMDFDPRVVQANQDVLIFYKRLRKERLL 620
BLAST of ATP-dependent DNA helicase PIF1 vs. UniProt/SwissProt
Match: sp|Q0R4F1|PIF1_XENLA (ATP-dependent DNA helicase PIF1 OS=Xenopus laevis OX=8355 GN=pif1 PE=2 SV=1) HSP 1 Score: 414.461 bits (1064), Expect = 7.156e-135 Identity = 252/606 (41.58%), Postives = 362/606 (59.74%), Query Frame = 3
Query: 234 QYGYPLVDMQIFERFVTEGKATIKLPKSSVQIMINNCPPGDLKLFLSTLKYKTKIAVNTVQXXXXXXXXXXXXPRSISSLDTISPIRKSD---KFKLQNKALENHFPDINPITPEKKRPLRDINEDFNTPPNKKLKIKQYTSTPNAKTSIKEIDNIFDSSEKLKKSATEFDVSLNDSGLTEYYQLSNEQRKIIECVRSGENVFYTGSAGTGKTYLLKKIIGTLVPQVXXXXXXXXXXXXLIDGCTLHNFAGIGLKMESLTASANIIMRKPQLLFQWRKCKHLIIDEISLISAEFFDHLDQVARVVRNNIKVPFGGIQLIISGDFFQLPPILPRNADKHYKFVFQSKVFEQAFKEIHFLTEVKRQSDQEFVAILSEIRMGRCSEATEKRLRTTANQNIERNGIIPTQLCTHKDDVESKNQSNLNSLPGEPEDFFAIDSIPGHRNTVDSVCMVPFKLTLKVGAQVMLLKNIDIAGGLANGSRGVVVGFEPAIKNRYPLVRFSSGKVYTMKPESFSVSISSSGQTLLIARSQIPLKLAYSISIHKSQGMTLDCVDLYLANTFECGQAYVALSRCKSLDSLRVMSWSNHCVKANHDVIKFFNKYQTDDIL 2042
Q + + +Q+F RF+ +GKA++ L ++Q++I+NCP LK F+ TL K + A + + ++ L +PR +TISP++K D +++ KA N TP K + L + +K T T ++ + I ++ S+K S + + +LS EQ ++ V SG NVF+TGSAGTGK+YLLK+I+G L P+ T TASTG+AA I G TLH FAGIG SL + R P + W CKHLIIDEIS++ EFFD L+ VAR VR + PFGGIQLI+ GDF QLPP+ A KF FQ K + + LTEV+RQ+D+ F+++L IR+GRC++ ++L T N +ER+GI+ T+LCTHKDDVE N+ L LPGE + A+DS P T+++ C V ++ LK GAQVML KN+D++ GL NG+RGVV+ FE KN P+VRF G +KP+ + + + ++R Q+PLKLA++ISIHKSQGM+LDCV++ L+ FE GQAYVALSR ++L+ LRVM + V+AN V++F+++ Q + L
Sbjct: 57 QQNFVIKQIQLFTRFIRDGKASVVLLPENIQLLISNCPADKLKHFMKTLLIKHE-AGKKEKPVNERTRLLSGLPRM---FETISPVQKKDVEQANEMRAKA--------NSETPVKGKGLSHKGVNGGNRCQQK-----RTRTESSNSLIADLR----PSKKPTLS------------MPKQIRLSTEQSLVLNTVLSGRNVFFTGSAGTGKSYLLKRIVGALPPKSTYATASTGVAACHIGGTTLHAFAGIGSGKASLEQCIELAKR-PGVRQHWTSCKHLIIDEISMVEGEFFDKLEAVARAVRGKDE-PFGGIQLIVCGDFLQLPPVT--QASSQTKFCFQGKSWRKCIHLTMELTEVRRQTDKNFISLLQAIRLGRCTDDVARQLLQTTNHKVERDGILATRLCTHKDDVEITNERRLQQLPGESHSYEALDSDPMLVKTINAQCPVNQQIQLKKGAQVMLAKNLDVSRGLVNGARGVVIKFEEGNKN-LPVVRFLCGVTEVIKPDRW---VFKGHGGIYLSRQQLPLKLAWAISIHKSQGMSLDCVEISLSRVFESGQAYVALSRARNLEGLRVMDFDPKVVRANPYVLQFYHQMQKERAL 621
BLAST of ATP-dependent DNA helicase PIF1 vs. UniProt/SwissProt
Match: sp|Q1HG60|PIF1_RAT (ATP-dependent DNA helicase PIF1 OS=Rattus norvegicus OX=10116 GN=Pif1 PE=2 SV=1) HSP 1 Score: 387.497 bits (994), Expect = 2.125e-124 Identity = 241/610 (39.51%), Postives = 346/610 (56.72%), Query Frame = 3
Query: 243 YPLVDMQIFERFVTEGKATIKLPK------SSVQIMINNCPPGDLKLFLSTLKYKTKIAVNTVQXXXXXXXXXXXXPRSISSLDTISP--IRKSDKFKLQNKALENHFPDINPITPEKKRPLRDINEDFNTPPNKKLKIKQYTSTPNAKTSIKEIDNIFDSSEKLKKSATEFDVSLNDSGLTEYYQLSNEQRKIIECVRSGENVFYTGSAGTGKTYLLKKIIGTLVPQVXXXXXXXXXXXXLIDGCTLHNFAGIGLKMESLTASANIIMRKPQLLFQWRKCKHLIIDEISLISAEFFDHLDQVARVVRNNIKVPFGGIQLIISGDFFQLPPILPRNADKHYKFVFQSKVFEQAFKEIHFLTEVKRQSDQEFVAILSEIRMGRCSEATEKRLRTTANQNIERNGIIPTQLCTHKDDVESKNQSNLNSLPGEPEDFFAIDSIPGHRNTVDSVCMVPFKLTLKVGAQVMLLKNIDIAGGLANGSRGVVVGFEPAIKNRYPLVRFSSGKVYTMKPESFSVSISSSGQTLLIARSQIPLKLAYSISIHKSQGMTLDCVDLYLANTFECGQAYVALSRCKSLDSLRVMSWSNHCVKANHDVIKFFNKYQTDDILGL 2048
+PL +++F RF G++T++LP SVQ+++++CPP L+ FL TL+ K +A + L PR ++ + P +R++ K + ALE + P + PL P KKL++ P++K +LS EQ ++ V G+++F+TGSAGTGK+YLLK I+G+L P T TASTG+AA I G TLH FAGIG L A + +P + W C+ L+IDEIS++ A+FFD L+ VAR VR K PFGGIQLII GDF QLPP+ +H +F FQ+K + + LTEV RQ+DQ F+++L +R+GRCS+ ++LR TA + R+GII T+LCTH+DDV N+ L LPG+ F AIDS P T+D+ C V L LK+GAQVML+KN+ ++ GL NG+RGVVVGFE + P VRF G ++ + ++V + + GQ L +R Q+PL+LA+++SIHKSQGM+LDCV++ L F GQAYVALSR +SL LRV+ + V+ + V++F+ + L L
Sbjct: 69 FPLRAVRLFTRFAAVGRSTLRLPADGVPRAGSVQLLLSDCPPERLRRFLRTLRLKLAVAPGP-GPASARAQLLGPRPRDFVTISPVQPEELRRAAATKAPDSALEKRPMESQPSMEAPRWPL----------PVKKLRM------PSSKP-----------------------------------KLSEEQAAVLRMVLKGQSIFFTGSAGTGKSYLLKHILGSLPPTGTVATASTGVAACHIGGTTLHAFAGIGSGQAPL-AQCVALAHRPGVRQGWLNCQRLVIDEISMVEADFFDKLEAVARAVRQQ-KKPFGGIQLIICGDFLQLPPVT--KGSQHPRFCFQAKSWRKCVPVTLELTEVWRQADQTFISLLKAVRLGRCSDEVTRQLRATAAHKVGRDGIIATRLCTHQDDVALTNEKRLKELPGDVHSFEAIDSDPELSRTLDAQCPVGRVLQLKLGAQVMLVKNLAVSRGLVNGARGVVVGFESEGRG-LPRVRFLCGITEVIRTDRWTVQV-TGGQYL--SRQQLPLQLAWAMSIHKSQGMSLDCVEISLGRVFASGQAYVALSRARSLQGLRVLDFDPTVVRCDSRVLQFYATLRQGRGLSL 618
BLAST of ATP-dependent DNA helicase PIF1 vs. UniProt/SwissProt
Match: sp|Q80SX8|PIF1_MOUSE (ATP-dependent DNA helicase PIF1 OS=Mus musculus OX=10090 GN=Pif1 PE=1 SV=2) HSP 1 Score: 382.489 bits (981), Expect = 2.766e-122 Identity = 240/610 (39.34%), Postives = 345/610 (56.56%), Query Frame = 3
Query: 243 YPLVDMQIFERFVTEGKATIKLP------KSSVQIMINNCPPGDLKLFLSTLKYKTKIAVNTVQXXXXXXXXXXXXPRSISSLDTISP--IRKSDKFKLQNKALENHFPDINPITPEKKRPLRDINEDFNTPPNKKLKIKQYTSTPNAKTSIKEIDNIFDSSEKLKKSATEFDVSLNDSGLTEYYQLSNEQRKIIECVRSGENVFYTGSAGTGKTYLLKKIIGTLVPQVXXXXXXXXXXXXLIDGCTLHNFAGIGLKMESLTASANIIMRKPQLLFQWRKCKHLIIDEISLISAEFFDHLDQVARVVRNNIKVPFGGIQLIISGDFFQLPPILPRNADKHYKFVFQSKVFEQAFKEIHFLTEVKRQSDQEFVAILSEIRMGRCSEATEKRLRTTANQNIERNGIIPTQLCTHKDDVESKNQSNLNSLPGEPEDFFAIDSIPGHRNTVDSVCMVPFKLTLKVGAQVMLLKNIDIAGGLANGSRGVVVGFEPAIKNRYPLVRFSSGKVYTMKPESFSVSISSSGQTLLIARSQIPLKLAYSISIHKSQGMTLDCVDLYLANTFECGQAYVALSRCKSLDSLRVMSWSNHCVKANHDVIKFFNKYQTDDILGL 2048
+PL +++F RF G++T++LP SVQ+++++CPP L+ FL TL+ K +A + L PR ++ + P ++++ K + ALE + T + PL P KKL++ P+ K +LS EQ ++ V G+++F+TGSAGTGK+YLLK I+G+L P T TASTG+AA I G TLH FAGIG L A + +P + W C+ L+IDEIS++ A+FFD L+ VAR VR K PFGGIQLII GDF QLPP+ + +F FQ+K + + I LTEV RQ+DQ F+++L +R+GRCS+ ++LR TA + R+GI+ T+LCTH+DDV N+ L +LPG+ F AIDS P T+D+ C V L LK+GAQVML+KN+ ++ GL NG+RGVVVGFE + P VRF G ++ + ++V + + GQ L +R Q+PL+LA++ISIHKSQGM+LDCV++ L F GQAYVALSR +SL LRV+ + V+ + V+ F+ + L L
Sbjct: 82 FPLRAVRLFTRFAATGRSTLRLPTDGVPGAGSVQLLLSDCPPERLRRFLRTLRLKLAVAPGP-GPASARAQLLGPRPRDFVTISPVQPEELQRAAATKAPDSALEKRPMESQTSTEAPRWPL----------PVKKLRM------PSTKP-----------------------------------KLSEEQAAVLRMVLKGQSIFFTGSAGTGKSYLLKHILGSLPPTGTVATASTGVAACHIGGTTLHAFAGIGSGQAPL-AQCMALANRPGVRQGWLNCQRLVIDEISMVEADFFDKLEAVARAVRQQ-KKPFGGIQLIICGDFLQLPPVT--KGSQQPQFCFQAKSWRRCVPVILELTEVWRQADQTFISLLQAVRLGRCSDEVTRQLRATAAHKVGRDGIVATRLCTHQDDVALTNEKWLKALPGDVHSFEAIDSDPELSRTLDAQCPVSRVLQLKLGAQVMLVKNLAVSRGLVNGARGVVVGFESEGRG-LPRVRFLCGITEVIRTDRWTVQV-TGGQYL--SRQQLPLQLAWAISIHKSQGMSLDCVEISLGRVFASGQAYVALSRARSLQGLRVLDFDPTVVRCDSRVLHFYATLRQGRGLSL 631
BLAST of ATP-dependent DNA helicase PIF1 vs. UniProt/SwissProt
Match: sp|Q9H611|PIF1_HUMAN (ATP-dependent DNA helicase PIF1 OS=Homo sapiens OX=9606 GN=PIF1 PE=1 SV=2) HSP 1 Score: 377.481 bits (968), Expect = 1.838e-120 Identity = 234/612 (38.24%), Postives = 338/612 (55.23%), Query Frame = 3
Query: 243 YPLVDMQIFERFVTEGKATIKLPK------SSVQIMINNCPPGDLKLFLSTLKYKTKIAVNTVQXXXXXXXXXXXXPRSISSLDTISPIRKSDKFKLQNKALENHFPDIN----PITPEKKRPLRDINEDFNTPPNKKLKIKQYTSTPNAKTSIKEIDNIFDSSEKLKKSATEFDVSLNDSGLTEYYQLSNEQRKIIECVRSGENVFYTGSAGTGKTYLLKKIIGTLVPQVXXXXXXXXXXXXLIDGCTLHNFAGIGLKMESLTASANIIMRKPQLLFQWRKCKHLIIDEISLISAEFFDHLDQVARVVRNNIKVPFGGIQLIISGDFFQLPPILPRNADKHYKFVFQSKVFEQAFKEIHFLTEVKRQSDQEFVAILSEIRMGRCSEATEKRLRTTANQNIERNGIIPTQLCTHKDDVESKNQSNLNSLPGEPEDFFAIDSIPGHRNTVDSVCMVPFKLTLKVGAQVMLLKNIDIAGGLANGSRGVVVGFEPAIKNRYPLVRFSSGKVYTMKPESFSVSISSSGQTLLIARSQIPLKLAYSISIHKSQGMTLDCVDLYLANTFECGQAYVALSRCKSLDSLRVMSWSNHCVKANHDVIKFFNKYQTDDILGL 2048
+PL ++F RF G++T++LP +VQ+++++CPP D A + L PR TISP++ ++ +L+ PD P+ P+ E P L +K+ S P+ K QLS EQ ++ V G+++F+TGSAGTGK+YLLK+I+G+L P T TASTG+AA I G TLH FAGIG L A + ++P + W C+ L+IDEIS++ A+ FD L+ VAR VR K PFGGIQLII GDF QLPP+ + +F FQSK +++ LT+V RQ+DQ F+++L +R+GRCS+ ++L+ TA+ + R+GI+ T+LCTH+DDV N+ L LPG+ F A+DS P +T+D+ C V L LK+GAQVML+KN+ ++ GL NG+RGVVVGFE A P VRF G + + ++V ++ GQ L++R Q+PL+LA+++SIHKSQGMTLDCV++ L F GQAYVALSR +SL LRV+ + V+ + V+ F+ + L L
Sbjct: 69 FPLRAARLFTRFAEAGRSTLRLPAHDTPGAGAVQLLLSDCPP-DRLRRFLRTLRLKLAAAPGPGPASARAQLLGPRPRDFV---TISPVQPEER-RLRAA---TRVPDTTLVKRPVEPQAG------AEPSTEAPRWPLPVKRL-SLPSTKP-----------------------------------QLSEEQAAVLRAVLKGQSIFFTGSAGTGKSYLLKRILGSLPPTGTVATASTGVAACHIGGTTLHAFAGIGSGQAPL-AQCVALAQRPGVRQGWLNCQRLVIDEISMVEADLFDKLEAVARAVRQQNK-PFGGIQLIICGDFLQLPPVT--KGSQPPRFCFQSKSWKRCVPVTLELTKVWRQADQTFISLLQAVRLGRCSDEVTRQLQATASHKVGRDGIVATRLCTHQDDVALTNERRLQELPGKVHRFEAMDSNPELASTLDAQCPVSQLLQLKLGAQVMLVKNLSVSRGLVNGARGVVVGFE-AEGRGLPQVRFLCGVTEVIHADRWTVQ-ATGGQ--LLSRQQLPLQLAWAMSIHKSQGMTLDCVEISLGRVFASGQAYVALSRARSLQGLRVLDFDPMAVRCDPRVLHFYATLRRGRSLSL 622
BLAST of ATP-dependent DNA helicase PIF1 vs. TrEMBL
Match: A0A401PM54 (ATP-dependent DNA helicase PIF1 OS=Scyliorhinus torazame OX=75743 GN=PIF1 PE=3 SV=1) HSP 1 Score: 434.491 bits (1116), Expect = 7.376e-140 Identity = 264/600 (44.00%), Postives = 369/600 (61.50%), Query Frame = 3
Query: 243 YPLVDMQIFERFVTEGKATIKLPKSSVQIMINNCPPGDLKLFLSTLKYKTKIAVNTVQXXXXXXXXXXXXPRSISSLDTISPIRKSDKFKLQNKALENHFPDINPITPEKKRPLRDINEDFNTPPNKKLKIKQYTSTPNAKTSIKEIDNIFDSSEKLKKSATEFDVSLNDSGLTEYYQLSNEQRKIIECVRSGENVFYTGSAGTGKTYLLKKIIGTLVPQVXXXXXXXXXXXXLIDGCTLHNFAGIGLKMESLTASANIIMRKPQLLFQWRKCKHLIIDEISLISAEFFDHLDQVARVVRNNIKVPFGGIQLIISGDFFQLPPILPRNADKHYKFVFQSKVFEQAFKEIHFLTEVKRQSDQEFVAILSEIRMGRCSEATEKRLRTTANQNIERNGIIPTQLCTHKDDVESKNQSNLNSLPGEPEDFFAIDSIPGHRNTVDSVCMVPFKLTLKVGAQVMLLKNIDIAGGLANGSRGVVVGFEPAIKNRYPLVRFSSGKVYTMKPESFSVSISSSGQTLLIARSQIPLKLAYSISIHKSQGMTLDCVDLYLANTFECGQAYVALSRCKSLDSLRVMSWSNHCVKANHDVIKFFNKYQTDDIL 2042
+ L ++Q+F +F+ +GK++IKL ++QI+++NCPP LK+ TL K A + + + L +PRS DTISP++ D K N+ L++ PI+ + + N+ P+K++K + S+ +N + KL K T + +L+ EQ I+ + G+NVF+TGSAGTGK++LLK+I+G L P+ T TASTG+AA I G TLH FAGIG L + R +L QW+ C+HLIIDEIS+I EFFD L+ VARVVRN PFGGIQLI+ GDF QLPP+ +N + KF FQ+K +++ LT+V+RQ+D++F+A+L IR+GRC E +L TANQ I+R+GI+ T+LCTHKDDVE NQ L LPGE F A+DS P T+++ C L LK GAQV+L KN+DI G+ NG+RGVVV F+ K P VRF G T+KPE + I + G T L R Q+PLKLA++ISIHKSQGM+LDCV++ LA FE GQAYVALSR +SL+ LRVM + + V AN DV++F+++ + + +L
Sbjct: 76 FALREIQLFTQFIRDGKSSIKLIPENIQILVSNCPPDKLKVLFKTLAIKYA-ADKISRPVSGRTRLLSTLPRS---FDTISPVQVQD-VKQANQ-LQSQGTVQTPISAKGLK-----NQVGIVGPSKQIKRSRTISS----------ENCLVTEMKLSKKPT--------LTMPVIRKLTKEQSHILGMILKGKNVFFTGSAGTGKSFLLKRIVGALPPKNTYTTASTGVAACHIGGTTLHAFAGIGSGKAPLQQCVELAQRSG-ILKQWQSCRHLIIDEISMIEGEFFDKLEAVARVVRN-CNEPFGGIQLIVCGDFLQLPPVSRKN--EKSKFCFQAKSWQKCIHVNMELTDVRRQTDKKFIAMLQAIRLGRCPEEVAVQLIKTANQKIDRDGILATRLCTHKDDVELTNQQKLQQLPGESHTFEAVDSDPMLIQTLNTQCPAGQTLQLKKGAQVILTKNLDIERGMVNGARGVVVDFDLGNKT-LPRVRFLCGVTETIKPERW--MIKALGGTYL-TRHQLPLKLAWAISIHKSQGMSLDCVEISLARVFESGQAYVALSRARSLEGLRVMDFDSRVVHANADVLRFYSRLKKERVL 638
BLAST of ATP-dependent DNA helicase PIF1 vs. TrEMBL
Match: K1PV98 (ATP-dependent DNA helicase PIF1 OS=Crassostrea gigas OX=29159 GN=PIF1 PE=3 SV=1) HSP 1 Score: 432.95 bits (1112), Expect = 7.502e-140 Identity = 257/596 (43.12%), Postives = 366/596 (61.41%), Query Frame = 3
Query: 243 YPLVDMQIFERFVTEGKATIKLPKSSVQIMINNCPPGDLKLFLSTLKYKTKIAVNTVQXXXXXXXXXXXXPRSISSLDTISPIRKSDKFKLQNKALENHFPDINPITPEKKRPLRDINEDFNTPPNKKLKIKQYTSTPNAKTSIKEIDNIFDSSEKLKKSATEFDVSLNDSGLTEYYQLSNEQRKIIECVRSGENVFYTGSAGTGKTYLLKKIIGTLVPQVXXXXXXXXXXXXLIDGCTLHNFAGIGLKMESLTASANIIMRKPQLLFQWRKCKHLIIDEISLISAEFFDHLDQVARVVRNNIKVPFGGIQLIISGDFFQLPPILPRNADKHYKFVFQSKVFEQAFKEIHFLTEVKRQSDQEFVAILSEIRMGRCSEATEKRLRTTANQNIERNGIIPTQLCTHKDDVESKNQSNLNSLPGEPEDFFAIDSIPGHRNTVDSVCMVPFKLTLKVGAQVMLLKNIDIAGGLANGSRGVVVGFEPAIKNR-YPLVRFSSGKVYTMKPESFSVSISSSGQTLLIARSQIPLKLAYSISIHKSQGMTLDCVDLYLANTFECGQAYVALSRCKSLDSLRVMSWSNHCVKANHDVIKFFNKYQ 2027
+ L D+ I ++F +GK+TIK+P ++ ++I+N PP L FL L TK ++ S++ KL + + F +I+P+T + L+ ++E K+L+ K TP K + K + D + + + SLN S + +LS EQ I++ V G+NVF+TGSAGTGK++L+++IIG+L PQ T TASTG+AA I G TLH FAGIG L + R PQ+ QWRKC HL+IDEIS++S FFD L+ VARVVR N PFGGIQLII GDF QLPP+ + DK F FQ+K + + + L EV+RQ+D F++IL IR+G+CSE T + LR T + I++NGI+ T+LCTHK+DV NQ +L L GE + F A+D +++ ++ + VP K+ LKVGAQVML KN+D+ GL NG+RGVVVGF+ KN+ YPLV+F SG+ ++P ++ +S + + I R Q+PLKLA++ISIHKSQGMTLDCV++ L+ FE GQ YVALSR KSL+ LR++ + CV AN DV++F++K +
Sbjct: 53 FALRDLTIHKKFAKDGKSTIKIPDRNMNLLISNSPPDKLIYFLKLLS--TKFECGRLKGFI------------------------SERKKLLSD-VPKSFEEISPLTI---KDLKTVHE----ARAKELESKPDMFTPKGKGTGKRKRDDDDKENRPVQRSKLMCSSLNPSSIALPVKLSKEQSTILDAVLKGKNVFFTGSAGTGKSFLMRRIIGSLPPQHTYATASTGVAACHIGGTTLHAFAGIGSGSAPLEQCVQLASR-PQIAQQWRKCHHLVIDEISMVSGAFFDKLETVARVVRKNDN-PFGGIQLIICGDFLQLPPV-TKGTDKKT-FCFQAKSWSRCVQVNMELKEVRRQNDLSFISILQNIRLGKCSEETHQILRQTVHHEIQKNGILATRLCTHKEDVNKINQYHLGKLQGEEKTFVAVDGEEAYKDQLEVLSPVPKKVVLKVGAQVMLAKNLDVQRGLVNGARGVVVGFDK--KNQDYPLVKFVSGEEEVIRPVRWTFKVSGA---VFINRKQVPLKLAWAISIHKSQGMTLDCVEMSLSRVFEAGQTYVALSRAKSLEGLRILDFDRSCVLANPDVLRFYHKLE 605
BLAST of ATP-dependent DNA helicase PIF1 vs. TrEMBL
Match: A0A4W3GQR2 (ATP-dependent DNA helicase PIF1 OS=Callorhinchus milii OX=7868 GN=pif1 PE=3 SV=1) HSP 1 Score: 432.95 bits (1112), Expect = 1.269e-139 Identity = 256/604 (42.38%), Postives = 369/604 (61.09%), Query Frame = 3
Query: 243 YPLVDMQIFERFVTEGKATIKLPKSSVQIMINNCPPGDLKLFLSTLKYKTKIAVNTVQXXXXXXXXXXXXPRSISSLDTISPIRKSD-KFKLQNKALENHFPDINPITPEKKRPLRDINEDFNTPPNKKLKIKQYTSTPNAKTSIKEIDNIFDSSEKLKKSATEFDVSLNDSGLTEYYQLSNEQRKIIECVRSGENVFYTGSAGTGKTYLLKKIIGTLVPQVXXXXXXXXXXXXLIDGCTLHNFAGIGLKMESLTASANIIMRKPQLLFQWRKCKHLIIDEISLISAEFFDHLDQVARVVRNNIKVPFGGIQLIISGDFFQLPPILPRNADKHYKFVFQSKVFEQAFKEIHF---LTEVKRQSDQEFVAILSEIRMGRCSEATEKRLRTTANQNIERNGIIPTQLCTHKDDVESKNQSNLNSLPGEPEDFFAIDSIPGHRNTVDSVCMVPFKLTLKVGAQVMLLKNIDIAGGLANGSRGVVVGFEPAIKNRYPLVRFSSGKVYTMKPESFSVSISSSGQTLLIARSQIPLKLAYSISIHKSQGMTLDCVDLYLANTFECGQAYVALSRCKSLDSLRVMSWSNHCVKANHDVIKFFNKYQTDDIL 2042
+ L ++Q+F RF+ +GK+ IK+ +VQI+I+NCPP L++ + TL K + + + L ++P++ D ISP++ D K + + L+N ++ P+ N N P +++K + TS+ N + + KK+ V +LS EQ ++ V G+NVF+TGSAGTGK++LLK+I+G L P+ T TASTG+AA I G TLH+FAGIG + + R P +L W+ CKHLIIDEIS+++ EFFD L+ VAR+VR +I+ PFGGIQLI+ GDF QLPP++ N K F FQ+K + K IH LTEV+RQ+D+ F+AIL IR+GRC+E +L TA+ I+++GI+ T+LCTHKDDVE N+ L LPG+ F A DS P T+++ C L LK GAQVML KN+D+ G+ NG+RGVV+GFE K P VRF G T+KPE +++ SS + + R Q+PL+LA++ISIHKSQGM+LDCV++ LA FE GQAYVALSR +SL+ LRVM + V+AN DV++F+++ + + +
Sbjct: 58 FVLREIQLFTRFIRDGKSCIKIVPDNVQILISNCPPDKLQVLVKTLAIKYGADKKSSKQASDRTRLLSVLPKT---FDNISPVQLHDVKRANELRMLQNVHTPVSTKAPK--------NRMGNACPGRQVKRPRTTSSENCPVT---------GLKPAKKAILSMPVQ---------RKLSKEQSHVLRTVLCGKNVFFTGSAGTGKSFLLKRIVGALPPKSTYTTASTGVAACHIGGTTLHSFAGIGSGKAPMQQCVELAQR-PGVLQHWQNCKHLIIDEISMVNGEFFDKLEAVARIVRKSIE-PFGGIQLIVCGDFLQLPPVIRTNEKK--TFCFQAKSWR---KCIHLNIELTEVRRQTDKAFIAILQAIRIGRCTEEVAAQLIKTASHKIDQDGILATRLCTHKDDVELTNERRLQHLPGKVHTFNASDSDPMLVQTLNAQCPAGQTLELKKGAQVMLTKNLDVQRGMVNGARGVVLGFESENKA-LPQVRFLCGVTETIKPERWTIKASSG---IYLTRQQLPLRLAWAISIHKSQGMSLDCVEISLARVFESGQAYVALSRARSLEGLRVMDFDPKVVRANPDVLQFYSRMKRERLF 621
BLAST of ATP-dependent DNA helicase PIF1 vs. TrEMBL
Match: A0A4W3H7L5 (ATP-dependent DNA helicase PIF1 OS=Callorhinchus milii OX=7868 GN=pif1 PE=3 SV=1) HSP 1 Score: 432.565 bits (1111), Expect = 1.966e-139 Identity = 253/601 (42.10%), Postives = 367/601 (61.06%), Query Frame = 3
Query: 243 YPLVDMQIFERFVTEGKATIKLPKSSVQIMINNCPPGDLKLFLSTLKYKTKIAVNTVQXXXXXXXXXXXXPRSISSLDTISPIRKSD-KFKLQNKALENHFPDINPITPEKKRPLRDINEDFNTPPNKKLKIKQYTSTPNAKTSIKEIDNIFDSSEKLKKSATEFDVSLNDSGLTEYYQLSNEQRKIIECVRSGENVFYTGSAGTGKTYLLKKIIGTLVPQVXXXXXXXXXXXXLIDGCTLHNFAGIGLKMESLTASANIIMRKPQLLFQWRKCKHLIIDEISLISAEFFDHLDQVARVVRNNIKVPFGGIQLIISGDFFQLPPILPRNADKHYKFVFQSKVFEQAFKEIHFLTEVKRQSDQEFVAILSEIRMGRCSEATEKRLRTTANQNIERNGIIPTQLCTHKDDVESKNQSNLNSLPGEPEDFFAIDSIPGHRNTVDSVCMVPFKLTLKVGAQVMLLKNIDIAGGLANGSRGVVVGFEPAIKNRYPLVRFSSGKVYTMKPESFSVSISSSGQTLLIARSQIPLKLAYSISIHKSQGMTLDCVDLYLANTFECGQAYVALSRCKSLDSLRVMSWSNHCVKANHDVIKFFNKYQTDDIL 2042
+ L ++Q+F RF+ +GK+ IK+ +VQI+I+NCPP L++ + TL K + + + L ++P++ D ISP++ D K + + L+N ++ P+ N N P +++K + TS+ N + + KK+ V +LS EQ ++ V G+NVF+TGSAGTGK++LLK+I+G L P+ T TASTG+AA I G TLH+FAGIG + + R P +L W+ CKHLIIDEIS+++ EFFD L+ VAR+VR +I+ PFGGIQLI+ GDF QLPP++ N K F FQ+K + + LTEV+RQ+D+ F+AIL IR+GRC+E +L TA+ I+++GI+ T+LCTHKDDVE N+ L LPG+ F A DS P T+++ C L LK GAQVML KN+D+ G+ NG+RGVV+GFE K P VRF G T+KPE +++ SS + + R Q+PL+LA++ISIHKSQGM+LDCV++ LA FE GQAYVALSR +SL+ LRVM + V+AN DV++F+++ + + +
Sbjct: 65 FVLREIQLFTRFIRDGKSCIKIVPDNVQILISNCPPDKLQVLVKTLAIKYGADKKSSKQASDRTRLLSVLPKT---FDNISPVQLHDVKRANELRMLQNVHTPVSTKAPK--------NRMGNACPGRQVKRPRTTSSENCPVT---------GLKPAKKAILSMPVQ---------RKLSKEQSHVLRTVLCGKNVFFTGSAGTGKSFLLKRIVGALPPKSTYTTASTGVAACHIGGTTLHSFAGIGSGKAPMQQCVELAQR-PGVLQHWQNCKHLIIDEISMVNGEFFDKLEAVARIVRKSIE-PFGGIQLIVCGDFLQLPPVIRTNEKK--TFCFQAKSWRKCIHLNIELTEVRRQTDKAFIAILQAIRIGRCTEEVAAQLIKTASHKIDQDGILATRLCTHKDDVELTNERRLQHLPGKVHTFNASDSDPMLVQTLNAQCPAGQTLELKKGAQVMLTKNLDVQRGMVNGARGVVLGFESENKA-LPQVRFLCGVTETIKPERWTIKASSG---IYLTRQQLPLRLAWAISIHKSQGMSLDCVEISLARVFESGQAYVALSRARSLEGLRVMDFDPKVVRANPDVLQFYSRMKRERLF 628
BLAST of ATP-dependent DNA helicase PIF1 vs. TrEMBL
Match: A0A1S3QZA8 (ATP-dependent DNA helicase PIF1 OS=Salmo salar OX=8030 GN=pif1 PE=3 SV=1) HSP 1 Score: 431.409 bits (1108), Expect = 5.688e-139 Identity = 260/610 (42.62%), Postives = 362/610 (59.34%), Query Frame = 3
Query: 243 YPLVDMQIFERFVTEGKATIKLPKSSVQIMINNCPPGDLKLFLSTLKYKTKIAVNTVQXXXXXXXXXXXXPRSISSLDTISPIRKSDKFKLQNKALENHFPDINPITPEKKRPLRDINEDFNTPP-NKKLKIKQYTSTPNAKTSIKEIDNIFDSSEKLKKSATEFDVS----LNDS-----GLTEYYQLSNEQRKIIECVRSGENVFYTGSAGTGKTYLLKKIIGTLVPQVXXXXXXXXXXXXLIDGCTLHNFAGIGLKMESLTASANIIMRKPQLLFQWRKCKHLIIDEISLISAEFFDHLDQVARVVRNNIKVPFGGIQLIISGDFFQLPPILPRNADKHYKFVFQSKVFEQAFKEIHFLTEVKRQSDQEFVAILSEIRMGRCSEATEKRLRTTANQNIERNGIIPTQLCTHKDDVESKNQSNLNSLPGEPEDFFAIDSIPGHRNTVDSVCMVPFKLTLKVGAQVMLLKNIDIAGGLANGSRGVVVGFEPAIKNRYPLVRFSSGKVYTMKPESFSVSISSSGQTLLIARSQIPLKLAYSISIHKSQGMTLDCVDLYLANTFECGQAYVALSRCKSLDSLRVMSWSNHCVKANHDVIKFFNKYQTDDIL 2042
Y L + Q+F RFV +GK T+KL ++Q++I++CPP L +FL TL K + + T P+ +K K L F I+P+ + + ++ N P ++ L ++ T N T + E++K+ T+ D S L+ S L +L+ EQ ++ V SG+NVF+TGSAGTGK++LLK+I+G+L P+ T TASTG+AA I G TLHNFAGIG L + R P +L W C+HLIIDEIS++ A+FFD L+ +AR +R + + PFGGIQLI+ GDF QLPP+ + DK F FQS+ + + LTEV+RQ+DQ F+++L +R+GR +E +L +A IER+GI+ T+LCTHKDDVE N + L LPG F A+DS P T+DS V L LKVGAQVML KN+D+ GL NG+RGVVV F+P K P VRF G +KPE + + +G L ++R Q+PLKLA++ISIHKSQGMTLDCV++ LA FE GQAYVALSR KSL+ LRVM + H V+A+ DV+ F++K + + +L
Sbjct: 59 YTLKEFQLFTRFVKDGKCTVKLLPENIQVLISDCPPHLLNIFLKTLSIKHQ------------------------AWQTSKPVTDREKLK---ACLPRSFETISPLQQKDVQKANELRSKVNAPVLSRGLVVR----TGNKMTGGPQ-------GEQVKRPRTDCDSSPLKALHPSKKPTLSLPTARKLNKEQAAVLSAVLSGKNVFFTGSAGTGKSFLLKRIVGSLPPKSTYATASTGVAACHIGGTTLHNFAGIGSGSAPLEQCIELAQR-PGVLQHWTSCRHLIIDEISMVEAQFFDKLEAIARSLRRSTE-PFGGIQLIVCGDFLQLPPV-SKGKDK-AAFCFQSRSWRKCIHLNMELTEVRRQTDQSFISLLQAVRVGRVTEEFTAKLMKSAYHKIERDGILATRLCTHKDDVELTNDNKLQQLPGSVRVFEAVDSDPALVKTIDSQSPVSRLLQLKVGAQVMLTKNLDVQRGLVNGARGVVVDFQPG-KLGLPRVRFLCGTTEALKPERW---MFKAGGGLYLSRQQLPLKLAWAISIHKSQGMTLDCVEISLARVFERGQAYVALSRAKSLEGLRVMDFDPHVVRADPDVLHFYSKLRKERLL 622
BLAST of ATP-dependent DNA helicase PIF1 vs. Ensembl Cavefish
Match: pif1 (PIF1 5'-to-3' DNA helicase homolog (S. cerevisiae) [Source:NCBI gene;Acc:326729]) HSP 1 Score: 259.996 bits (663), Expect = 4.808e-78 Identity = 161/421 (38.24%), Postives = 237/421 (56.29%), Query Frame = 3
Query: 243 YPLVDMQIFERFVTEGKATIKLPKSSVQIMINNCPPGDLKLFLSTLKYKTKIAVNTVQXXXXXXXXXXXXPRSISSLDTISPIRKSDKFKLQNKALENHFPDINPITPEKKRPLRDINEDFNTPPNKKLKIKQYTSTPNAKTSIKEIDNIFDSSEKLKKSATEFDVSLNDSGLTEYYQLSNEQRKIIECVRSGENVFYTGSAGTGKTYLLKKIIGTLVPQVXXXXXXXXXXXXLIDGCTLHNFAGIGLKMESLTASANIIMRKPQLLFQWRKCKHLIIDEISLISAEFFDHLDQVARVVRNNIKVPFGGIQLIISGDFFQLPPILPRNADKHYKFVFQSKVFEQAFKEIHFLTEVKRQSDQEFVAILSEIRMGRCSEATEKRLRTTANQNIERNGIIPTQLCTHKDDVESKNQSNLNSLPG 1505
+ L D Q+F RF +GK T+KL Q++I++CPP L FL TL+ K + A + + + L +PRS +TISP+++ D K N + + PL+ + + K Q P A + + + KK V+ +LS EQ +++ V SG+N+F+TGSAGTGK++LLK+I+G+L P+ T TASTG+AA I G TLH+FAGIG L + R P +L W CKHLIIDE+S++ A+FFD L+ +AR +R + + PFGGIQLI+ GDF QLPP+ + F FQ++ + + LTEV+RQ+D+ F+++L +R+GR +E +L +AN IER+GI+ T+LCTHKDDVE N++ L LPG
Sbjct: 62 FMLRDFQLFTRFAKDGKCTVKLEPDGTQVLISDCPPDRLSGFLRTLRIKHE-ASRDRKPVSDRVRLKATLPRS---FNTISPLQQKDVQK------------ANELRSKVNAPLQLKGLSVRSGLSNKAGGSQQVKRPRADCDTSPVKALHPN----KKPVLTLPVA---------RKLSKEQAAVLDAVLSGKNIFFTGSAGTGKSFLLKRIVGSLPPKSTYATASTGVAACHIGGTTLHSFAGIGSGSAPLEQCLELAQR-PGVLQHWTGCKHLIIDEVSMVEAQFFDKLEAIARSIRRSTE-PFGGIQLIVCGDFLQLPPVT--KGKEKPSFCFQARSWRKCIHANMELTEVRRQTDRAFISLLQAVRVGRVTEEVTTQLLKSANNRIERDGILATRLCTHKDDVELTNENKLKQLPG 449
BLAST of ATP-dependent DNA helicase PIF1 vs. Ensembl Cavefish
Match: ENSAMXT00000033960.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02001433.1:35398:42348:-1 gene:ENSAMXG00000030720.1 transcript:ENSAMXT00000033960.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 125.561 bits (314), Expect = 4.702e-29 Identity = 151/558 (27.06%), Postives = 242/558 (43.37%), Query Frame = 3
Query: 519 NKALENHFPDINPITPEKKRPLRDINEDFNTPPNKKLKIK-QYTSTPNAKTSIKEIDNIFDSSEKLKKSATEFDV----SLNDSGLTEYYQLSNEQRKII------ECVRSGEN-----VFY--TGSAGTGKTYLLKKIIGTL------VPQVXXXXXXXXXXXXL--IDGCTLHNFAGIGLK-MESLTASANIIMRKP---------QLLFQWRKCKHLIIDEISLISAEFFDHLDQVARVVRNNIKVPFGGIQLIISGDFFQLPPILPRNADKHYKFVFQSKVFEQAFKEIHF----LTEVKRQSDQ-EFVAILSEIRMGRCSEATEKRLRTTANQNIERNGIIPTQLC---------THKDDVESKNQSNLNSLPGEPEDFFAIDSIPGHRNTVDSVCMVPFK---------LTLKVGAQVMLLKNIDIAGGLANGSRGVVVGFEPAIKNRYPLVR--------FSSGKVYTMK----PESFSVSISSSGQTLL---IARSQIPLKLAYSISIHKSQGMTLDCVDLYLANTFECGQAYVALSRCKSLDSLRVMSW 1970
+KA+EN E+ P+ D + P N+ ++++ P T I E D++ D + ++ T V +N + + + YQ NE + + C R +N FY G+AGTGK++L+K + +P V + L GC HN G L + L S ++ P ++ + + +IIDEIS++S F +++ + ++ N K PFG I ++ GDF QLPP+ V++ V + F HF LTE+ RQ D F +L+ +R+ + EA T A++ + I P + C T+K+ V+ N + + S + A D R+ PFK L + VGA+VML +NID+ GL NGS G V +N P+V+ +G+ + + ++F V I ++L R Q P+KLA++ + HK QGMT + L FE G AYVALSR SL L ++ +
Sbjct: 1157 DKAIENF---------EQNGPIEDAWTNL-APTNELIRLECMIEQEPTDPTDITEADDVPDFAPSTSENRTAMPVIEAPQVNPAVIRKMYQSLNETQAAVFYEIRDWCKRKVQNENPEPFFYYVAGTAGTGKSHLIKSVYAEATKILQKLPCVREEADMSKPTVLLSAFTGCASHNINGKTLHALLKLPRS----LKPPYQGLGNSLDEIRANFSNLQIMIIDEISMVSKPLFAYVNWRLQQIKGNNK-PFGNISIMAVGDFQQLPPL----GKAKPLCVYEDHVLD--FWRDHFKMITLTEIMRQKDDLAFAELLNRLRVKQKHEAL-----TDADRRMLEQVIKPIENCPPEALHIFPTNKE-VDDFNSAAITSRFSDITKIDAEDYKKDPRSGQMQRQGAPFKGEKGDLMDTLQVAVGARVMLTRNIDVESGLVNGSFGKVAKIITQTRNSVPVVQKIGVELDNADAGRKHRSEVPGETDNF-VYIEREEESLKKKGTVRRQFPMKLAFACTAHKVQGMTTHSAVVSLKKIFEPGMAYVALSRTTSLSGLNIIDF 1686
BLAST of ATP-dependent DNA helicase PIF1 vs. Ensembl Cavefish
Match: ENSAMXT00000047502.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02001744.1:2246:5800:1 gene:ENSAMXG00000038708.1 transcript:ENSAMXT00000047502.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 114.775 bits (286), Expect = 7.183e-26 Identity = 130/484 (26.86%), Postives = 202/484 (41.74%), Query Frame = 3
Query: 741 SLNDSGLTEYYQLSNEQRKIIECVRSGE----NVFYTGSAGTGKTYLLKKI---IGTLVPQVXXXXXXXXXXXXLIDGC--------TLHNFAGIGLKME-SLTASANIIMRKPQLLFQWRKCKHLIIDEISLISAEFFDHLDQVARVVRNNIKV-PFGGIQLIISGDFFQLPPILPRNADKHYKFVFQSKVFEQAFKEIHFLTEVKRQSDQEFVAILSEIRMGRCS-----------------EATEKRLRTTANQNIERNGI------IPTQLCTHKDDVESKNQSNLNSLPGEPEDFFAIDSIPGHRNTVDSVCMVPFKLTLKVGAQVMLLKNIDIAGGLANGSRGVVVGF---EPAIKNRYPLVRFSSGKV------YTMKPESFSVS---------ISSSGQTLLIARSQIPLKLAYSISIHKSQGMTLDCVDLYLANTFECGQAYVALSRCKSLDSLRVMSWSNHCVKANHDVIKFFN 2018
SLN +YQ+ ++ IE VR + ++F TG AGTGKT+L++ I L+ QV++ + + G T+H+ IG ++ T + ++LF LIIDEIS++ ++ R ++ PFG + +I GDFFQLPP+ Y ++ F + LT + RQ D F IL+ +R S E T N +E + + P +C D E Q+ L GH + V + C+ P KL + GA+VML+KNID+ GL NG G V + + + V+F KV T P S ++S G R Q PLKLA++ ++HK QG+T+ + L F GQAYVALSR ++L L + +++ + ++ + N
Sbjct: 488 SLNSEQKDIFYQV---RQWCIEKVRGKQPEPFHLFITGGAGTGKTHLIRSIHYEATRLLSQVSSAPDKLSVLLTAPTGIAAFNLGAGTIHSTFSIGTDVKLPYTPLGEEKLNTLRVLFS--DLHILIIDEISMVDHSLLAYIHGRLRQIKQTGDYSPFGKVSIIAVGDFFQLPPV---KGKPLYLENAPLDLWNGMFSKSE-LTTIVRQKDSAFAEILNRLRTHHRSTPLSLQDVKTLKQRETGETTSALHIFATNAQVEEHNVRQIIASCPEHICVEAQDYEKNRQTGKMQLKN------------GHFSKVFNSCL-PEKLLIGEGARVMLIKNIDVTDGLVNGVCGTVTDIAHDKNSTLVKTVFVQFDDQKVGQNSRKRTPPPRHIIHSTPINLEEERVNSKGGL----RRQFPLKLAWACTVHKVQGLTVKQAVVSLKKIFSPGQAYVALSRVETLSGLIIQDFADKAIYCRSNIQECIN 945
BLAST of ATP-dependent DNA helicase PIF1 vs. Ensembl Cavefish
Match: ENSAMXT00000047800.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02001870.1:12181:15308:-1 gene:ENSAMXG00000035975.1 transcript:ENSAMXT00000047800.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 110.153 bits (274), Expect = 1.713e-24 Identity = 126/485 (25.98%), Postives = 207/485 (42.68%), Query Frame = 3
Query: 741 SLNDSGLTEYYQLSNEQRKIIECVRSGE----NVFYTGSAGTGKTYLLKKI---IGTLVPQVXXXXXXXXXXXXL--------IDGCTLHNFAGIGLKME-SLTASANIIMRKPQLLFQWRKCKHLIIDEISLISAEFFDHLDQVARVVRNNIKV-PFGGIQLIISGDFFQLPPILPRNAD-KHYKFVFQSKVFEQAFKEIHFLTEVKRQSDQEFVAILSEIRMG----------------RCSEATEKRLRTTA-NQNIERNGI------IPTQLCTHKDDVESKNQSNLNSLPGEPEDFFAIDSIPGHRNTVDSVCMVPFKLTLKVGAQVMLLKNIDIAGGLANGSRGVVVGF---EPAIKNRYPLVRFSSGKV------YTMKPESFSVS---------ISSSGQTLLIARSQIPLKLAYSISIHKSQGMTLDCVDLYLANTFECGQAYVALSRCKSLDSLRVMSWSNHCVKANHDVIKFFN 2018
SLN +YQ+ ++ +E VR + ++F TG AGTGKT+L++ I L+ QV++ + + ++ T+H+ IG ++ T + ++L LIIDEIS++ ++ R ++ PFG + +I GDFFQLPP+ + ++ + +F ++ LT + RQ D F IL+ +R R + T L A N +E + + P +C D E Q+ L GH + V + C+ P KL + GA+VML+KNID+ GL NG G V + + + V+F KV T P S ++S G R Q PLKLA++ ++HK QG+T+ + L F GQAYVALS+ ++L L + +++ + ++ + N
Sbjct: 198 SLNSEQKDTFYQV---RQWCLEKVRGKQPEPFHMFITGGAGTGKTHLIRSIHYEATRLLSQVSSAPDKVSVLLTAPTGIAAFNLEAGTIHSTFSIGTDVKLPYTPLGEEKLNTLRVLLS--DLHILIIDEISMVDHSLLAYIHGRLRQIKQTGDYSPFGKVSVIAVGDFFQLPPVKGKPLYLENAPLDLWNGIFSKSE-----LTTIVRQKDSAFAEILNRLRTHHRSTPLSLQDVHALKQRETGETSSALHIFATNAQVEEHNVKQIIAFCPEHICVEAQDYEKNRQTGKMQLKN------------GHFSKVFNSCL-PEKLLIGEGARVMLIKNIDVTDGLVNGVCGTVTDITHDKNSTLVKTVFVQFDDQKVGQNSRKRTPPPRHILHSTPVNLEEERVNSKGG----LRRQFPLKLAWACTVHKVQGLTVKQAVVSLKKIFAPGQAYVALSQVETLSGLIIQDFADKAIYCRSNIQECIN 655
BLAST of ATP-dependent DNA helicase PIF1 vs. Ensembl Sea Lamprey
Match: pif1 (PIF1 5'-to-3' DNA helicase homolog (S. cerevisiae) [Source:NCBI gene;Acc:326729]) HSP 1 Score: 265.388 bits (677), Expect = 1.197e-83 Identity = 137/296 (46.28%), Postives = 189/296 (63.85%), Query Frame = 3
Query: 1131 RVVRNNIKVPFGGIQLIISGDFFQLPPI-LPRNADKHYKFVFQSKVFEQAFKEIHFLTEVKRQSDQEFVAILSEIRMGRCSEATEKRLRTTANQNIERNGIIPTQLCTHKDDVESKNQSNLNSLPGEPEDFFAIDSIPGHRNTVDSVCMVPFKLTLKVGAQVMLLKNIDIAGGLANGSRGVVVGFEPAIKNRYPLVRFSSGKVYTMKPESFSVSISSSGQTLLIARSQIPLKLAYSISIHKSQGMTLDCVDLYLANTFECGQAYVALSRCKSLDSLRVMSWSNHCVKANHDVIKFF 2015
R VR + PFGGIQL++SGDF QLPP+ R + + F Q+K + + LT+V RQ DQ F+ +L +R GRC++ + L T + IE++GI+ T+LCTH+DDV+ N L LPGE F A+DS P +D++C V L L+ GAQVML KN+D+ GL NG+RGVV F K PLVRF+ G V ++ E + V +G +I R Q+PL+LA++ISIHKSQGM+LDCV++ L FECGQAYVALSR +SL LRV+ + V+A+ DV++F+
Sbjct: 1 RTVRGRDE-PFGGIQLVLSGDFLQLPPVSRDRTVKRTFSFHVQAKSWRRCVHLAMELTQVHRQKDQAFIRVLQMVRTGRCTDEVTRCLVATKDNAIEKDGILATRLCTHRDDVQLINTCQLAKLPGETHMFDALDSDPSQAKALDAMCPVEASLQLRRGAQVMLTKNLDVQRGLVNGARGVVTDFSTE-KFGLPLVRFACGVVQAVQVERWVV---RAGGGNVITRKQLPLRLAWAISIHKSQGMSLDCVEVCLGRVFECGQAYVALSRARSLQGLRVVDFDPCVVRADPDVLRFY 291
BLAST of ATP-dependent DNA helicase PIF1 vs. Ensembl Yeast
Match: RRM3 (DNA helicase involved in rDNA replication and Ty1 transposition; binds to and suppresses DNA damage at G4 motifs in vivo; relieves replication fork pauses at telomeric regions; structurally and functionally related to Pif1p [Source:SGD;Acc:S000001073]) HSP 1 Score: 233.032 bits (593), Expect = 3.866e-67 Identity = 167/516 (32.36%), Postives = 258/516 (50.00%), Query Frame = 3
Query: 711 LKKSATEFDVSLNDSGLTEYYQLSNEQRKIIEC-VRSGENVFYTGSAGTGKTYLLKKIIGTLVPQVXXXXXXXXXXXXL----IDGCTLHNFAGIGLKMESLTASANIIMRKPQLLFQWRKCKHLIIDEISLISAEFFDHLDQVARVVRNNIKVPFGGIQLIISGDFFQLPPILPRNADKHYKFVFQSKVFEQAFKEIHFLTEVKRQSDQEFVAILSEIRMGRCSEATEKRLRTTANQNIE-RNGIIPTQLCTHKDDVESKNQSNLNSLPGEPEDFFAIDSIPG-HRNTVDSVCMVPFKLTLKVGAQVMLLKNI-DIAGGLANGSRGVVVGF--------------------------------EPAIK---------NRYPLVRFSSGKV---YTMK----PESFSVSISSSGQTLLIARSQIP--------------------LKLAYSISIHKSQGMTLDCVDLYLANTFECGQAYVALSRCKSLDSLRVMSWSNHCVKANHDVIKFFNKYQT 2030
L+K+ + D S+N + L+ EQ +++ V+ NVFYTGSAGTGK+ +L+ II L + + + L I G TLH ++GIG+ +++ I + LL WR K LIIDEIS++ D L+Q+AR +R N PFGGIQL+++GDFFQLPP+ ++ KF F+S+++++ ++ LT+V RQ D + + IL+ IR G + K +R N++I+ +GI PT+L + +VE N L SLPG+ +F A+D+ P ++ +DS MV + LK AQVM+LKN D+ L NGS G V+ F P +K N PL R K+ Y +K E F + G+ I +P L L +++SIHK+QG T+ + + L FE GQ YVALSR ++D+L+V+++ ++ N V F+ + +T
Sbjct: 211 LRKTVSNMD-SMNHRSASSPVVLTMEQERVVNLIVKKRTNVFYTGSAGTGKSVILQTIIRQLSSLYGKESIAITASTGLAAVTIGGSTLHKWSGIGIGNKTIDQLVKKIQSQKDLLAAWRYTKVLIIDEISMVDGNLLDKLEQIARRIRKNDD-PFGGIQLVLTGDFFQLPPVAKKDEHNVVKFCFESEMWKRCIQKTILLTKVFRQQDNKLIDILNAIRYGELTVDIAKTIRNL-NRDIDYADGIAPTELYATRREVELSNVKKLQSLPGDLYEFKAVDNAPERYQAILDSSLMVEKVVALKEDAQVMMLKNKPDVE--LVNGSLGKVLFFVTESLVVKMKEIYKIVDDEVVMDMRLVSRVIGNPLLKESKEFRQDLNARPLARLERLKILINYAVKISPHKEKFPYVRWTVGKNKYIHELMVPERFPIDIPRENVGLERTQIPLMLCWALSIHKAQGQTIQRLKVDLRRIFEAGQVYVALSRAVTMDTLQVLNFDPGKIRTNERVKDFYKRLET 721
BLAST of ATP-dependent DNA helicase PIF1 vs. Ensembl Yeast
Match: PIF1 (DNA helicase, potent G-quadruplex DNA binder/unwinder; possesses strand annealing activity; promotes DNA synthesis during break-induced replication; important for crossover recombination; translation from different start sites produces mitochondrial and nuclear forms; nuclear form is a catalytic inhibitor of telomerase; mitochondrial form involved in DNA repair and recombination; mutations affect Zn, Fe homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells [Source:SGD;Acc:S000004526]) HSP 1 Score: 194.126 bits (492), Expect = 8.537e-53 Identity = 119/311 (38.26%), Postives = 176/311 (56.59%), Query Frame = 3
Query: 777 LSNEQRKIIECVRSGENVFYTGSAGTGKTYLLKKIIGTLVPQVXXXXXXXXXXXXL----IDGCTLHNFAGIGLKMESLTASANIIMRKPQLLFQWRKCKHLIIDEISLISAEFFDHLDQVARVVRNNIKVPFGGIQLIISGDFFQLPPILPRNADKHYKFVFQSKVFEQAFKEIHFLTEVKRQ-SDQEFVAILSEIRMGRCSEATEKRLRTTANQNIERNGIIPTQLCTHKDDVESKNQSNLNSLPGEPEDFFAIDSIPGHRNTVDSVC----MVPFKLTLKVGAQVMLLKNIDIAGGLANGSRGVVVGF 1682
LS EQ II+ +G N+FYTGSAGTGK+ LL+++I L + + L I G T+H+FAGIGL + R + L +W L++DEIS++ AE D LD +AR +R N + PFGGIQLI GDFFQLPP+ ++ ++ KF F+SK +++ K L +V RQ D +F+ +L+ +R+G + TE+ + + + + + IIP +L + + +VE N S L+ LPG+ F AID + + P +L LKVGAQVM++KN+D L NGS G V+ F
Sbjct: 237 LSKEQESIIKLAENGHNIFYTGSAGTGKSILLREMIKVLKGIYGRENVAVTASTGLAACNIGGITIHSFAGIGLGKGDADKLYKKVRRSRKHLRRWENIGALVVDEISMLDAELLDKLDFIARKIRKNHQ-PFGGIQLIFCGDFFQLPPV-SKDPNRPTKFAFESKAWKEGVKMTIMLQKVFRQRGDVKFIDMLNRMRLGNIDDETEREFKKLS-RPLPDDEIIPAELYSTRMEVERANNSRLSKLPGQVHIFNAIDGGALEDEELKERLLQNFLAPKELHLKVGAQVMMVKNLD--ATLVNGSLGKVIEF 542 HSP 2 Score: 90.5077 bits (223), Expect = 3.132e-19 Identity = 50/113 (44.25%), Postives = 71/113 (62.83%), Query Frame = 3
Query: 1689 AIKNRYPLVRFSSGKVYT----MKPESFSVSISSSGQTLLIARSQIPLKLAYSISIHKSQGMTLDCVDLYLANTFECGQAYVALSRCKSLDSLRVMSWSNHCVKANHDVIKFF 2015
A K R PLVRF + + T ++PE ++ I + L++R Q+PL LA+S+SIHKSQG TL V + L FE GQAYVALSR S + L+V+++ +KA+ VI F+
Sbjct: 651 AGKRRLPLVRFKASDMSTRMVLVEPEDWA--IEDENEKPLVSRVQLPLMLAWSLSIHKSQGQTLPKVKVDLRRVFEKGQAYVALSRAVSREGLQVLNFDRTRIKAHQKVIDFY 761
BLAST of ATP-dependent DNA helicase PIF1 vs. Ensembl Nematostella
Match: EDO45143 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RTT7]) HSP 1 Score: 95.9005 bits (237), Expect = 1.962e-22 Identity = 72/221 (32.58%), Postives = 100/221 (45.25%), Query Frame = 3
Query: 243 YPLVDMQIFERFVTEGKATIKLPKSSVQIMINNCPPGDLKLFLSTLKYKTKIAVNTVQXXXXXXXXXXXXPRS--ISSLDTISPIRKSDKFKLQNKALENHFPDINPITPEKKRPLRDINEDFNTPPNKKLKIKQYTSTPNAKTSIKEIDNIFDSSEKLKKSATEFDVSLNDSGLTEYYQLSNEQRKIIECVRSGENVFYTGSAGTGKTYLLKKIIGTLVP 899
Y L ++ I +RFV+EGKATIKL +Q+++ NCPP L FL I V + + A ++ RS + DTISP+ + D K TP KKR +IN N K+ K + P K +I + F + EQ +I VRSG NVF+TGSAGTGK++LL+++IG L P
Sbjct: 57 YRLREITIHKRFVSEGKATIKLTTLGIQLLLANCPPAKLTAFLRC------IVVKLAKKKEDGFASERTRMRSDLPKNFDTISPLVEKDFLKRT--------------TPNKKRAFGEINGGNNGQTPKRCKREALNDVPRKKLNIASLQMKFTA----------------------------EQSAVINAVRSGRNVFFTGSAGTGKSFLLRRLIGMLPP 229
BLAST of ATP-dependent DNA helicase PIF1 vs. Ensembl Nematostella
Match: EDO36997 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SGY7]) HSP 1 Score: 53.5286 bits (127), Expect = 2.554e-7 Identity = 56/191 (29.32%), Postives = 86/191 (45.03%), Query Frame = 3
Query: 1263 VFEQAFKEIHFLTEVKRQ--SDQEFVAILSEIRMGRCSEATE---KRLRTTANQNIERNGIIPTQLCTHKDDVESKNQSNLNSLPGEPEDFFAIDSIPGHRNTVDSVCMVPFKLTLKVGAQVMLLKNIDIAGGLANGSRGVVVGFEPAIKNRYPLVRFSSGKVYTMKPESFSVSISSSGQTLLIARSQIPL 1820
+F+ AF L ++ RQ ++ EFV L E+R+G S+++ KRL I ++ TQ+ + + + N + LPG+ F A D NT + C L LK G +VML+ N + L NGS GV VG E + LV F + KP+ + S ++ R+Q P
Sbjct: 30 IFQHAFHHRFELLDIVRQDQAETEFVTCLKEVRIGYSSDSSTQLLKRLERDLPDEINQSA---TQIFFTRLEAQLYNLKRIRKLPGQILRFEASD----QGNTDNISCAAEKMLLLKPGCKVMLIYN--KSDELRNGSSGVFVGVE----SESVLVEFKD--IGITKPKRETWHKKSMIGEVVATRTQFPF 205
BLAST of ATP-dependent DNA helicase PIF1 vs. Ensembl Medaka
Match: pif1 (PIF1 5'-to-3' DNA helicase [Source:NCBI gene;Acc:101166662]) HSP 1 Score: 420.239 bits (1079), Expect = 3.482e-138 Identity = 254/603 (42.12%), Postives = 364/603 (60.36%), Query Frame = 3
Query: 243 YPLVDMQIFERFVTEGKATIKLPKSSVQIMINNCPPGDLKLFLSTLKYKTKIAVNTVQXXXXXXXXXXXXPRSISSLDTISPIRKSDKFK---LQNKALENHFPDINPITPEKKRPLRDINEDFNTPPNKKLKIKQYTSTPNAKTSIKEIDNIFDSSEKLKKSATEFDVSLNDSGLTEYYQLSNEQRKIIECVRSGENVFYTGSAGTGKTYLLKKIIGTLVPQVXXXXXXXXXXXXLIDGCTLHNFAGIGLKMESLTASANIIMRKPQLLFQWRKCKHLIIDEISLISAEFFDHLDQVARVVRNNIKVPFGGIQLIISGDFFQLPPILPRNADKHYKFVFQSKVFEQAFKEIHFLTEVKRQSDQEFVAILSEIRMGRCSEATEKRLRTTANQNIERNGIIPTQLCTHKDDVESKNQSNLNSLPGEPEDFFAIDSIPGHRNTVDSVCMVPFKLTLKVGAQVMLLKNIDIAGGLANGSRGVVVGFEPAIKNRYPLVRFSSGKVYTMKPESFSVSISSSGQTLLIARSQIPLKLAYSISIHKSQGMTLDCVDLYLANTFECGQAYVALSRCKSLDSLRVMSWSNHCVKANHDVIKFFNKYQTDDIL 2042
Y L + Q+F +FV +GK T+KL + Q++++NCPP L LFL TL K + A + + L +++ L +PRS + ISP+++ D K L++KA + P K+ ++ D ++ P K + PN K ++ +LS EQ ++ V G+NVF+TGSAGTGK++LLK+I+G+L P+ T +TASTG+AA I G TLHNFAGIG L + R P +L W C+HLIIDEIS++ A+FFD L+ VAR V+ + + PFGGIQLI+ GDF QLPP+ + F FQ++ + + + LTEV+RQ+DQ F+++L +R+GR +E +L T++ IER+G++ T+LCTHKDDVE N+ L LPG F A+DS P +D+ V + LKVGAQVML KN+D+A GL NG+RGVV FE K P VRF G ++PE + V S+SG + ++R+Q+PLKLA++ISIHKSQGMTLDCV++ LA FE GQAYVALSR +SL+ LRVM ++ H V+A+ DV+ F+ K + + +L
Sbjct: 59 YRLKEFQLFTKFVRDGKCTVKLLPENTQVLLSNCPPDRLSLFLKTLSIKHQ-AWRSCKPLSEREKLKAALPRS---FEAISPLQQKDIQKANELRSKA--SGGPADCTNRAGGKQQVKRGRGDTSSSPEK--------TNPNKKPTLT----------------------------LPSRRLSKEQTAVLSAVLRGKNVFFTGSAGTGKSFLLKRIMGSLPPKSTFVTASTGVAACHIGGTTLHNFAGIGSGSAPLEQCIELAQR-PSVLQHWTSCRHLIIDEISMVEAQFFDKLEAVARSVKRSTQ-PFGGIQLIVCGDFLQLPPV--SKGKEKASFCFQARCWRKVIQVNMELTEVRRQTDQNFISLLQAVRLGRVTEEISMKLMTSSYHRIERDGVLATRLCTHKDDVELTNEDKLQQLPGSVRLFEAVDSDPELVKNIDAHSPVSRLIQLKVGAQVMLTKNLDVARGLVNGARGVVEAFESEKKG-LPRVRFLCGVTEVLRPERW-VFKSASG--VHLSRTQLPLKLAWAISIHKSQGMTLDCVEISLARVFESGQAYVALSRARSLEGLRVMDFNPHVVRADPDVLLFYKKLRKERLL 611
BLAST of ATP-dependent DNA helicase PIF1 vs. Ensembl Medaka
Match: pif1 (PIF1 5'-to-3' DNA helicase [Source:NCBI gene;Acc:101166662]) HSP 1 Score: 419.853 bits (1078), Expect = 5.362e-138 Identity = 254/603 (42.12%), Postives = 364/603 (60.36%), Query Frame = 3
Query: 243 YPLVDMQIFERFVTEGKATIKLPKSSVQIMINNCPPGDLKLFLSTLKYKTKIAVNTVQXXXXXXXXXXXXPRSISSLDTISPIRKSDKFK---LQNKALENHFPDINPITPEKKRPLRDINEDFNTPPNKKLKIKQYTSTPNAKTSIKEIDNIFDSSEKLKKSATEFDVSLNDSGLTEYYQLSNEQRKIIECVRSGENVFYTGSAGTGKTYLLKKIIGTLVPQVXXXXXXXXXXXXLIDGCTLHNFAGIGLKMESLTASANIIMRKPQLLFQWRKCKHLIIDEISLISAEFFDHLDQVARVVRNNIKVPFGGIQLIISGDFFQLPPILPRNADKHYKFVFQSKVFEQAFKEIHFLTEVKRQSDQEFVAILSEIRMGRCSEATEKRLRTTANQNIERNGIIPTQLCTHKDDVESKNQSNLNSLPGEPEDFFAIDSIPGHRNTVDSVCMVPFKLTLKVGAQVMLLKNIDIAGGLANGSRGVVVGFEPAIKNRYPLVRFSSGKVYTMKPESFSVSISSSGQTLLIARSQIPLKLAYSISIHKSQGMTLDCVDLYLANTFECGQAYVALSRCKSLDSLRVMSWSNHCVKANHDVIKFFNKYQTDDIL 2042
Y L + Q+F +FV +GK T+KL + Q++++NCPP L LFL TL K + A + + L +++ L +PRS + ISP+++ D K L++KA + P K+ ++ D ++ P K + PN K ++ +LS EQ ++ V G+NVF+TGSAGTGK++LLK+I+G+L P+ T +TASTG+AA I G TLHNFAGIG L + R P +L W C+HLIIDEIS++ A+FFD L+ VAR V+ + + PFGGIQLI+ GDF QLPP+ + F FQ++ + + + LTEV+RQ+DQ F+++L +R+GR +E +L T++ IER+G++ T+LCTHKDDVE N+ L LPG F A+DS P +D+ V + LKVGAQVML KN+D+A GL NG+RGVV FE K P VRF G ++PE + V S+SG + ++R+Q+PLKLA++ISIHKSQGMTLDCV++ LA FE GQAYVALSR +SL+ LRVM ++ H V+A+ DV+ F+ K + + +L
Sbjct: 59 YRLKEFQLFTKFVRDGKCTVKLLPENTQVLLSNCPPDRLSLFLKTLSIKHQ-AWRSCKPLSEREKLKAALPRS---FEAISPLQQKDIQKANELRSKA--SGGPADCTNRAGGKQQVKRGRGDTSSSPEK--------TNPNKKPTLT----------------------------LPSRRLSKEQTAVLSAVLRGKNVFFTGSAGTGKSFLLKRIMGSLPPKSTFVTASTGVAACHIGGTTLHNFAGIGSGSAPLEQCIELAQR-PSVLQHWTSCRHLIIDEISMVEAQFFDKLEAVARSVKRSTQ-PFGGIQLIVCGDFLQLPPV--SKGKEKASFCFQARCWRKVIQVNMELTEVRRQTDQNFISLLQAVRLGRVTEEISMKLMTSSYHRIERDGVLATRLCTHKDDVELTNEDKLQQLPGSVRLFEAVDSDPELVKNIDAHSPVSRLIQLKVGAQVMLTKNLDVARGLVNGARGVVEAFESEKKG-LPRVRFLCGVTEVLRPERW-VFKSASG--VHLSRTQLPLKLAWAISIHKSQGMTLDCVEISLARVFESGQAYVALSRARSLEGLRVMDFNPHVVRADPDVLLFYKKLRKERLL 611
BLAST of ATP-dependent DNA helicase PIF1 vs. Ensembl Medaka
Match: ENSORLT00000017442.2 (pep primary_assembly:ASM223467v1:21:13302614:13303912:1 gene:ENSORLG00000013908.2 transcript:ENSORLT00000017442.2 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 126.331 bits (316), Expect = 1.201e-30 Identity = 116/424 (27.36%), Postives = 191/424 (45.05%), Query Frame = 3
Query: 828 VFYTGSAGTGKTYLLK-------KIIGTLVPQVXXXXXXXXXXXXL----IDGCTLHNFAGIGLKMESLTASANIIMRKPQLLFQWRKCKHLIIDEISLISAEFFDHLDQVARVVRNNIKV-PFGGIQLIISGDFFQLPPIL--PRNADKHYKFVFQSKVFEQAFKEIHFLTEVKRQSDQEFVAILSEIRMGRCSEATEKRLRTTANQNIERN---------GIIPTQLCTHKDDVESKNQSNLNSLPGEPEDFF------AIDSIPGHRNTVDSVCMVPFKLTLKVGAQVMLLKNIDIAGGLANGSRGVVVGFEPAIKNRYP---LVRFSS---GKVY-------------TMKPESFSVSISSSGQTLLIARSQIPLKLAYSISIHKSQGMTLDCVDLYLANTFECGQAYVALSRCKSLDSL 1955
+F TG AGTGK++L++ +++ L Q A++ + + T+H GIG + L S + + ++ + LIIDEIS++ ++ R ++ PFG + +I GDF+QLPP+ P A+K + ++ FK + LTE+ RQ DQ F +L+ +R + + +L + Q +++ I PT + ++E + N EDF + + GH V + C+ +L L V A+VML KN+DI GL NG+ G+V E + +P V+F GK+ + PE VS R Q PLKLA++ ++HK QG+T+D + F GQ YVA+SR ++++ L
Sbjct: 1 MFVTGGAGTGKSHLIRAIQYEATRLLSPLCLQPDAMSVLLTAPTGIAAYSLKAATIHTTFGIGTEFR-LPYSPLGEEKLNSMRVKYHDLQILIIDEISMVDHRLLTYIHGRLRQIKQTGDFSPFGKVSIIAVGDFYQLPPVKGKPLYAEKS-----ELNLWSMCFKVVE-LTEIVRQKDQAFAQMLNRLR----TRTKDTKLLPSDVQMLKQCETGEDSTALHIFPTNAQVFQHNLEQLKITCENYCEIVAEDFIPSKETGKLQLMSGHHKKVKNSCLEE-RLLLGVNARVMLCKNLDITDGLVNGACGIVTHIESYTEGGFPKYVYVKFDDEEIGKIARRTCAKADLPGSTAIGPEEERVSQKGG------MRRQFPLKLAWACTVHKVQGLTVDKAVVSFNKIFAAGQVYVAVSRVRTIEGL 406
BLAST of ATP-dependent DNA helicase PIF1 vs. Ensembl Medaka
Match: ENSORLT00000028913.1 (pep primary_assembly:ASM223467v1:10:27563519:27584335:-1 gene:ENSORLG00000027083.1 transcript:ENSORLT00000028913.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 128.257 bits (321), Expect = 7.403e-30 Identity = 120/446 (26.91%), Postives = 199/446 (44.62%), Query Frame = 3
Query: 816 SGEN-----VFYTGSAGTGKTYLLK-------KIIGTLVPQVXXXXXXXXXXXXL----IDGCTLHNFAGIGLKMESLTASANIIMRKPQLLFQWRKCKHLIIDEISLISAEFFDHLDQVARVVRNNIKV-PFGGIQLIISGDFFQLPPIL--PRNADKHYKFVFQSKVFEQAFKEIHFLTEVKRQSDQEFVAILSEIRMGRCSEATEKRLRTTANQNIERN---------GIIPTQLCTHKDDVESKNQSNLNSLPGEPEDFF------AIDSIPGHRNTVDSVCMVPFKLTLKVGAQVMLLKNIDIAGGLANGSRGVVVGFEPAIKNRYP---LVRFSS---GKVY-------------TMKPESFSVSISSSGQTLLIARSQIPLKLAYSISIHKSQGMTLDCVDLYLANTFECGQAYVALSRCKSLDSLRVMSWSNHCVKAN 1994
SG+N +F TG AGTGK++L++ +++ L Q A++ + + T+H GIG + L S + + ++ + LIIDEIS++ ++ R ++ PFG + +I GDF+QLPP+ P ADK + ++ FK + L E+ RQ DQ F +L+ +R + + +L + Q +++ I PT + ++E + N EDF + + GH V + C+ +L L V A+VML KN+DI GL NG+ G+V E + +P V+F GK+ ++PE VS R Q PLKLA++ ++HK QG+T+D + F GQ YVA+SR ++++ L + + + N
Sbjct: 2005 SGQNPDPLHMFVTGGAGTGKSHLIRAIQYEATRLLSPLCLQPDAVSVLLTAPTGIAAYSLKAATIHTTFGIGTEFR-LPYSPLGEEKLNSMRVKYHDLQILIIDEISMVDHRLLIYIHGRLRQIKQTGDFSPFGKVSIIAVGDFYQLPPVRGKPLYADKS-----ELNLWSMCFKVVE-LREIVRQKDQAFAQMLNRLR----TRTKDTKLLPSDVQMLKQCETGEDSTALHIFPTNAQVFQHNLEQLKITCENYCEIVAEDFIPSKETGKLQLMSGHHKKVKNSCLEE-RLLLGVNARVMLCKNLDITDGLVNGACGIVTHIESYTEGGFPKYVYVKFDDEEIGKIARRTCAKADLPGSTAIEPEEERVSQKGG------MRRQFPLKLAWACTVHKVQGLTVDKAVVSFNKIFAAGQVYVAVSRVRTIEGLIIRDFKEKAIYCN 2432
BLAST of ATP-dependent DNA helicase PIF1 vs. Ensembl Medaka
Match: ENSORLT00000031383.1 (pep primary_assembly:ASM223467v1:10:27563519:27584335:-1 gene:ENSORLG00000027083.1 transcript:ENSORLT00000031383.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 128.257 bits (321), Expect = 7.403e-30 Identity = 120/446 (26.91%), Postives = 199/446 (44.62%), Query Frame = 3
Query: 816 SGEN-----VFYTGSAGTGKTYLLK-------KIIGTLVPQVXXXXXXXXXXXXL----IDGCTLHNFAGIGLKMESLTASANIIMRKPQLLFQWRKCKHLIIDEISLISAEFFDHLDQVARVVRNNIKV-PFGGIQLIISGDFFQLPPIL--PRNADKHYKFVFQSKVFEQAFKEIHFLTEVKRQSDQEFVAILSEIRMGRCSEATEKRLRTTANQNIERN---------GIIPTQLCTHKDDVESKNQSNLNSLPGEPEDFF------AIDSIPGHRNTVDSVCMVPFKLTLKVGAQVMLLKNIDIAGGLANGSRGVVVGFEPAIKNRYP---LVRFSS---GKVY-------------TMKPESFSVSISSSGQTLLIARSQIPLKLAYSISIHKSQGMTLDCVDLYLANTFECGQAYVALSRCKSLDSLRVMSWSNHCVKAN 1994
SG+N +F TG AGTGK++L++ +++ L Q A++ + + T+H GIG + L S + + ++ + LIIDEIS++ ++ R ++ PFG + +I GDF+QLPP+ P ADK + ++ FK + L E+ RQ DQ F +L+ +R + + +L + Q +++ I PT + ++E + N EDF + + GH V + C+ +L L V A+VML KN+DI GL NG+ G+V E + +P V+F GK+ ++PE VS R Q PLKLA++ ++HK QG+T+D + F GQ YVA+SR ++++ L + + + N
Sbjct: 2005 SGQNPDPLHMFVTGGAGTGKSHLIRAIQYEATRLLSPLCLQPDAVSVLLTAPTGIAAYSLKAATIHTTFGIGTEFR-LPYSPLGEEKLNSMRVKYHDLQILIIDEISMVDHRLLIYIHGRLRQIKQTGDFSPFGKVSIIAVGDFYQLPPVRGKPLYADKS-----ELNLWSMCFKVVE-LREIVRQKDQAFAQMLNRLR----TRTKDTKLLPSDVQMLKQCETGEDSTALHIFPTNAQVFQHNLEQLKITCENYCEIVAEDFIPSKETGKLQLMSGHHKKVKNSCLEE-RLLLGVNARVMLCKNLDITDGLVNGACGIVTHIESYTEGGFPKYVYVKFDDEEIGKIARRTCAKADLPGSTAIEPEEERVSQKGG------MRRQFPLKLAWACTVHKVQGLTVDKAVVSFNKIFAAGQVYVAVSRVRTIEGLIIRDFKEKAIYCN 2432
BLAST of ATP-dependent DNA helicase PIF1 vs. Planmine SMEST
Match: SMESG000057255.1 (SMESG000057255.1) HSP 1 Score: 1324.3 bits (3426), Expect = 0.000e+0 Identity = 668/668 (100.00%), Postives = 668/668 (100.00%), Query Frame = 3
Query: 57 MSHSSITASVNLQSLGKDNNTVINTKQFKQALVESVREDDGQVMYVRITLISTSKKVTGQYGYPLVDMQIFERFVTEGKATIKLPKSSVQIMINNCPPGDLKLFLSTLKYKTKIAVNTVQXXXXXXXXXXXXPRSISSLDTISPIRKSDKFKLQNKALENHFPDINPITPEKKRPLRDINEDFNTPPNKKLKIKQYTSTPNAKTSIKEIDNIFDSSEKLKKSATEFDVSLNDSGLTEYYQLSNEQRKIIECVRSGENVFYTGSAGTGKTYLLKKIIGTLVPQVXXXXXXXXXXXXLIDGCTLHNFAGIGLKMESLTASANIIMRKPQLLFQWRKCKHLIIDEISLISAEFFDHLDQVARVVRNNIKVPFGGIQLIISGDFFQLPPILPRNADKHYKFVFQSKVFEQAFKEIHFLTEVKRQSDQEFVAILSEIRMGRCSEATEKRLRTTANQNIERNGIIPTQLCTHKDDVESKNQSNLNSLPGEPEDFFAIDSIPGHRNTVDSVCMVPFKLTLKVGAQVMLLKNIDIAGGLANGSRGVVVGFEPAIKNRYPLVRFSSGKVYTMKPESFSVSISSSGQTLLIARSQIPLKLAYSISIHKSQGMTLDCVDLYLANTFECGQAYVALSRCKSLDSLRVMSWSNHCVKANHDVIKFFNKYQTDDILGLYEDF 2060
MSHSSITASVNLQSLGKDNNTVINTKQFKQALVESVREDDGQVMYVRITLISTSKKVTGQYGYPLVDMQIFERFVTEGKATIKLPKSSVQIMINNCPPGDLKLFLSTLKYKTKIAVNTVQDLKKKKALDKIIPRSISSLDTISPIRKSDKFKLQNKALENHFPDINPITPEKKRPLRDINEDFNTPPNKKLKIKQYTSTPNAKTSIKEIDNIFDSSEKLKKSATEFDVSLNDSGLTEYYQLSNEQRKIIECVRSGENVFYTGSAGTGKTYLLKKIIGTLVPQVTAITASTGIAASLIDGCTLHNFAGIGLKMESLTASANIIMRKPQLLFQWRKCKHLIIDEISLISAEFFDHLDQVARVVRNNIKVPFGGIQLIISGDFFQLPPILPRNADKHYKFVFQSKVFEQAFKEIHFLTEVKRQSDQEFVAILSEIRMGRCSEATEKRLRTTANQNIERNGIIPTQLCTHKDDVESKNQSNLNSLPGEPEDFFAIDSIPGHRNTVDSVCMVPFKLTLKVGAQVMLLKNIDIAGGLANGSRGVVVGFEPAIKNRYPLVRFSSGKVYTMKPESFSVSISSSGQTLLIARSQIPLKLAYSISIHKSQGMTLDCVDLYLANTFECGQAYVALSRCKSLDSLRVMSWSNHCVKANHDVIKFFNKYQTDDILGLYEDF
Sbjct: 1 MSHSSITASVNLQSLGKDNNTVINTKQFKQALVESVREDDGQVMYVRITLISTSKKVTGQYGYPLVDMQIFERFVTEGKATIKLPKSSVQIMINNCPPGDLKLFLSTLKYKTKIAVNTVQDLKKKKALDKIIPRSISSLDTISPIRKSDKFKLQNKALENHFPDINPITPEKKRPLRDINEDFNTPPNKKLKIKQYTSTPNAKTSIKEIDNIFDSSEKLKKSATEFDVSLNDSGLTEYYQLSNEQRKIIECVRSGENVFYTGSAGTGKTYLLKKIIGTLVPQVTAITASTGIAASLIDGCTLHNFAGIGLKMESLTASANIIMRKPQLLFQWRKCKHLIIDEISLISAEFFDHLDQVARVVRNNIKVPFGGIQLIISGDFFQLPPILPRNADKHYKFVFQSKVFEQAFKEIHFLTEVKRQSDQEFVAILSEIRMGRCSEATEKRLRTTANQNIERNGIIPTQLCTHKDDVESKNQSNLNSLPGEPEDFFAIDSIPGHRNTVDSVCMVPFKLTLKVGAQVMLLKNIDIAGGLANGSRGVVVGFEPAIKNRYPLVRFSSGKVYTMKPESFSVSISSSGQTLLIARSQIPLKLAYSISIHKSQGMTLDCVDLYLANTFECGQAYVALSRCKSLDSLRVMSWSNHCVKANHDVIKFFNKYQTDDILGLYEDF 668 The following BLAST results are available for this feature:
BLAST of ATP-dependent DNA helicase PIF1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 3
BLAST of ATP-dependent DNA helicase PIF1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of ATP-dependent DNA helicase PIF1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 2
BLAST of ATP-dependent DNA helicase PIF1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of ATP-dependent DNA helicase PIF1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of ATP-dependent DNA helicase PIF1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 4
BLAST of ATP-dependent DNA helicase PIF1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of ATP-dependent DNA helicase PIF1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of ATP-dependent DNA helicase PIF1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 4
BLAST of ATP-dependent DNA helicase PIF1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 1
BLAST of ATP-dependent DNA helicase PIF1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 2
BLAST of ATP-dependent DNA helicase PIF1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 2
BLAST of ATP-dependent DNA helicase PIF1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of ATP-dependent DNA helicase PIF1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 1
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30020142 ID=SMED30020142|Name=ATP-dependent DNA helicase PIF1|organism=Schmidtea mediterranea sexual|type=transcript|length=2112bpback to top protein sequence of SMED30020142-orf-1 >SMED30020142-orf-1 ID=SMED30020142-orf-1|Name=SMED30020142-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=669bp MSHSSITASVNLQSLGKDNNTVINTKQFKQALVESVREDDGQVMYVRITLback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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