Protein rolling stone

Overview
NameProtein rolling stone
Smed IDSMED30020068
Length (bp)948
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Protein rolling stone (SMED30020068) t-SNE clustered cells

Violin plots show distribution of expression levels for Protein rolling stone (SMED30020068) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Protein rolling stone (SMED30020068) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Protein rolling stone (SMED30020068) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30020068

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
gutSMED30020068h1SMcG0003043 Contig174GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
dorsal ventral margin of the whole animalSMED30020068h1SMcG0003043 Contig174GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30020068h1SMcG0003043 Contig174GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
gutSMED30020068h1SMcG0003043 dd_Smed_v4_2511_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Protein rolling stone vs. Ensembl Fly
Match: CG43778 (gene:FBgn0264308 transcript:FBtr0331503)

HSP 1 Score: 71.2478 bits (173), Expect = 2.683e-13
Identity = 60/212 (28.30%), Postives = 98/212 (46.23%), Query Frame = 3 
Query:  243 FLAYRIIIGIFGLAYVI---FDITWETINFYKHNIGYWFIYATNWAFVIFSFSMAILAANCVIR--------FFKPRYEFNDNYCHGIFLLYTITAVSTLTVDLVYWVALNAEKGLMSESEARIKHTIPTIIILVDLMINRIVLELAHXXXXXXXXXXXXXFNAIYYHSGGTRYDGKPYVYEAVNWTHTESATTACLIVIVFTLIIHNILFC 845
            +L YR I  I  LA ++    DI   T   ++H+   W+IY T+W  ++F    A LAA  V +        F   R        H  ++LYT   V    V + YW+ ++  +    ++   + H + TII+L+DL I    ++++H  +    G+ Y IF  IY+ +GGT    +  +Y  ++WT    A       I+FT  +H   FC
Sbjct:  551 YLFYRWITAITCLAALVCSLLDIG-RTDEHFEHHYAKWWIYLTHWG-LLFCTVQAWLAAWIVTQGMMVEREDFEIVRQAKKSRLHHLYWVLYTCATVYAFIVTMCYWLLVHNPEIHKIDALNIMVHVLNTIIMLIDLAIVGHPIKMSHAYFTTGIGLAYAIFTGIYFLAGGTDRKNQTAIYPMMDWTKPGKAIIVTACAIIFTFFVH---FC 757
[Close]
BLAST of Protein rolling stone vs. Ensembl Fly
Match: CG13101 (gene:FBgn0032084 transcript:FBtr0079784)

HSP 1 Score: 60.4622 bits (145), Expect = 4.245e-10
Identity = 59/220 (26.82%), Postives = 102/220 (46.36%), Query Frame = 3 
Query:  243 FLAYRIIIGIFGLAYVIFDITWETINFYKHNIGYW--FIYATNWAFVIFSFSMAILAANCVIRFFKPRYE---FNDNYCHGIFLLY----TITAVSTLTVDLVYWVALNAEKGLMSESEARIKHTIPTIIILVDLMINRIVLELAHXXXXXXXXXXXXXFNAIYYHSGGTRYDGKPYVYEAVNWTHTESATTACLIVIVFTLIIHNILFCITFIKFLLKE 875
            +L YR  + +F +  VI  +  ET +       YW  FIY TNW   +   +  + A    I  + P Y     N       F LY     I+ V ++ + ++YW  L        ++   + H   +I + +DL I    L L H+  P++ GV++ IF+ IY+  GG    GKPY+Y  ++W+  ++A T  + V++ +  I+ +LF    ++  L  
Sbjct:   47 YLFYRWFMAMFFVVVVIISMWPETDD----ATSYWLYFIYMTNWGIWMCMLTNVLGAILVTIWHYHPEYADKLLNMKSYFSYFRLYWGMHIISLVLSIVITIIYWSILYDANESALDATNVLTHAFNSICMFIDLWIVAHPLRLLHIFLPVLFGVVFAIFSYIYHLCGGINKKGKPYIYYVIDWSKPQNAFTTVVGVLLLSCCIYVLLFAFFKLRLFLHR 262
[Close]
BLAST of Protein rolling stone vs. Ensembl Fly
Match: CG13101 (gene:FBgn0032084 transcript:FBtr0079783)

HSP 1 Score: 60.4622 bits (145), Expect = 4.245e-10
Identity = 59/220 (26.82%), Postives = 102/220 (46.36%), Query Frame = 3 
Query:  243 FLAYRIIIGIFGLAYVIFDITWETINFYKHNIGYW--FIYATNWAFVIFSFSMAILAANCVIRFFKPRYE---FNDNYCHGIFLLY----TITAVSTLTVDLVYWVALNAEKGLMSESEARIKHTIPTIIILVDLMINRIVLELAHXXXXXXXXXXXXXFNAIYYHSGGTRYDGKPYVYEAVNWTHTESATTACLIVIVFTLIIHNILFCITFIKFLLKE 875
            +L YR  + +F +  VI  +  ET +       YW  FIY TNW   +   +  + A    I  + P Y     N       F LY     I+ V ++ + ++YW  L        ++   + H   +I + +DL I    L L H+  P++ GV++ IF+ IY+  GG    GKPY+Y  ++W+  ++A T  + V++ +  I+ +LF    ++  L  
Sbjct:   47 YLFYRWFMAMFFVVVVIISMWPETDD----ATSYWLYFIYMTNWGIWMCMLTNVLGAILVTIWHYHPEYADKLLNMKSYFSYFRLYWGMHIISLVLSIVITIIYWSILYDANESALDATNVLTHAFNSICMFIDLWIVAHPLRLLHIFLPVLFGVVFAIFSYIYHLCGGINKKGKPYIYYVIDWSKPQNAFTTVVGVLLLSCCIYVLLFAFFKLRLFLHR 262
[Close]
BLAST of Protein rolling stone vs. Ensembl Fly
Match: CG43778 (gene:FBgn0264308 transcript:FBtr0331505)

HSP 1 Score: 60.4622 bits (145), Expect = 5.397e-10
Identity = 60/212 (28.30%), Postives = 98/212 (46.23%), Query Frame = 3 
Query:  243 FLAYRIIIGIFGLAYVI---FDITWETINFYKHNIGYWFIYATNWAFVIFSFSMAILAANCVIR--------FFKPRYEFNDNYCHGIFLLYTITAVSTLTVDLVYWVALNAEKGLMSESEARIKHTIPTIIILVDLMINRIVLELAHXXXXXXXXXXXXXFNAIYYHSGGTRYDGKPYVYEAVNWTHTESATTACLIVIVFTLIIHNILFC 845
            +L YR I  I  LA ++    DI   T   ++H+   W+IY T+W  ++F    A LAA  V +        F   R        H  ++LYT   V    V + YW+ ++  +    ++   + H + TII+L+DL I    ++++H  +    G+ Y IF  IY+ +GGT    +  +Y  ++WT    A       I+FT  +H   FC
Sbjct:   39 YLFYRWITAITCLAALVCSLLDIG-RTDEHFEHHYAKWWIYLTHWG-LLFCTVQAWLAAWIVTQGMMVEREDFEIVRQAKKSRLHHLYWVLYTCATVYAFIVTMCYWLLVHNPEIHKIDALNIMVHVLNTIIMLIDLAIVGHPIKMSHAYFTTGIGLAYAIFTGIYFLAGGTDRKNQTAIYPMMDWTKPGKAIIVTACAIIFTFFVH---FC 245
[Close]
BLAST of Protein rolling stone vs. Ensembl Fly
Match: rost (gene:FBgn0011705 transcript:FBtr0331454)

HSP 1 Score: 55.4546 bits (132), Expect = 1.420e-8
Identity = 55/240 (22.92%), Postives = 105/240 (43.75%), Query Frame = 3 
Query:  207 FDSMWSRLNVKP-FLAYRIIIGIFGLAYVIFDITWETINFYKHNIGYWFIYATNWAFVIFSFSMAILAANCVIRFFKPRYE--------FNDNYCHGI---FLLYTITAVSTLTVDLVYWVALNAE--KGLMSESEARIKHTIPTIIILVDLMINRIVLELAHXXXXXXXXXXXXXFNAIYYHSGGTRYDGKPYVYEAVNWTHTESATTACLIVIVFTLIIHNILFCITFIKFLLKESYS 884
            + S W +  +   +L YR I  +F L   I  +  +  +      G +FIY TNW F + + +M I A       F  R                 G+   + LY +T    L +  VYWV L+ +  K +   + + I H + ++++L+D ++    L + H++Y +   + + +F  IY+  GGT   G  YVY  ++W +        + + +  +    +LF +  +K +   ++S
Sbjct:   31 YRSQWQKDEINTIYLLYRWIWALFFLGVYIMCVIVQFCD------GKFFIYMTNWGFGLCTITMLISAVQVTCWHFDVRSTRSLVQESGHKAETSRGLKIYWWLYNMTLSLALIISTVYWVFLHGKMNKPMRFPAISIITHGMNSVMMLIDFLVIAFPLRILHMVYGMSLAIFFFLFTLIYHLCGGTDEFGNHYVYPILDWNNPNRCMVTFVGIFLLIMCYWVLLFGLYKLKRMFNRAFS 264
[Close]
BLAST of Protein rolling stone vs. UniProt/SwissProt
Match: sp|O44252|ROST_DROME (Protein rolling stone OS=Drosophila melanogaster OX=7227 GN=rost PE=2 SV=2)

HSP 1 Score: 55.4546 bits (132), Expect = 1.423e-7
Identity = 55/240 (22.92%), Postives = 105/240 (43.75%), Query Frame = 3 
Query:  207 FDSMWSRLNVKP-FLAYRIIIGIFGLAYVIFDITWETINFYKHNIGYWFIYATNWAFVIFSFSMAILAANCVIRFFKPRYE--------FNDNYCHGI---FLLYTITAVSTLTVDLVYWVALNAE--KGLMSESEARIKHTIPTIIILVDLMINRIVLELAHXXXXXXXXXXXXXFNAIYYHSGGTRYDGKPYVYEAVNWTHTESATTACLIVIVFTLIIHNILFCITFIKFLLKESYS 884
            + S W +  +   +L YR I  +F L   I  +  +  +      G +FIY TNW F + + +M I A       F  R                 G+   + LY +T    L +  VYWV L+ +  K +   + + I H + ++++L+D ++    L + H++Y +   + + +F  IY+  GGT   G  YVY  ++W +        + + +  +    +LF +  +K +   ++S
Sbjct:   31 YRSQWQKDEINTIYLLYRWIWALFFLGVYIMCVIVQFCD------GKFFIYMTNWGFGLCTITMLISAVQVTCWHFDVRSTRSLVQESGHKAETSRGLKIYWWLYNMTLSLALIISTVYWVFLHGKMNKPMRFPAISIITHGMNSVMMLIDFLVIAFPLRILHMVYGMSLAIFFFLFTLIYHLCGGTDEFGNHYVYPILDWNNPNRCMVTFVGIFLLIMCYWVLLFGLYKLKRMFNRAFS 264
[Close]
BLAST of Protein rolling stone vs. TrEMBL
Match: G7YA02 (Protein rolling stone OS=Clonorchis sinensis OX=79923 GN=rost PE=4 SV=1)

HSP 1 Score: 102.449 bits (254), Expect = 2.333e-21
Identity = 81/253 (32.02%), Postives = 142/253 (56.13%), Query Frame = 3 
Query:  210 DSMWSRLNVKPFL--AYRIIIGIFGLAYVIFDITWETINFYKHNIGYWFIYATNWAFVIFSFSMAILAANCVIRFFKPRYEFNDNYCHG-IFLLYTITAVSTLTVDLVYWVAL-NAEKGLMSESEARIKHTIPTIIILVDLMINRIVLELAHXXXXXXXXXXXXXFNAIYYHSGGTRYDGKPYVYEAVNWTHTESATTACLIVIVFTLIIHNILFCITFIKFLL---------------KESYSDIKKSVNSD 911
            DS W  L +KP+L   YR+++ +  L ++ FDI  E  N++      WF+YATNW+F + + +  +LA  C+I +  PR +      +G I+L+Y I   + + V +VYWVAL N+  G+ S    ++KH++   + ++DL I+ I + LAH +YPI+ G+IY +F+ +++  GG    G   VY  +NW H ++A  +CL  ++F+ +IH +++   +I+  L               KE Y D++  V ++
Sbjct:   44 DSQW--LWMKPWLFTTYRVLLSLGCLVWICFDIPREIHNYFHDKTHIWFVYATNWSFGLLTLAFFLLALYCLI-YNCPRRKDATLILYGPIWLIYIIALDTIIVVSIVYWVALANSNLGVFSSIWGKLKHSLAACLAVLDLFISSIPVRLAHAVYPILIGLIYAVFSYLFWLGGGEGPLGVGQVYPGLNWIHPKTAILSCLCALLFSFLIHILIYVTYYIRVKLSTCVGGRQAETVKQMKEGYKDLRSDVENE 293
[Close]
BLAST of Protein rolling stone vs. TrEMBL
Match: A0A074ZRW1 (Uncharacterized protein OS=Opisthorchis viverrini OX=6198 GN=T265_03644 PE=4 SV=1)

HSP 1 Score: 102.449 bits (254), Expect = 2.581e-21
Identity = 78/251 (31.08%), Postives = 141/251 (56.18%), Query Frame = 3 
Query:  210 DSMWSRLNVKPFLAYRIIIGIFGLAYVIFDITWETINFYKHNIGYWFIYATNWAFVIFSFSMAILAANCVIRFFKPRYEFNDNYCHG-IFLLYTITAVSTLTVDLVYWVAL-NAEKGLMSESEARIKHTIPTIIILVDLMINRIVLELAHXXXXXXXXXXXXXFNAIYYHSGGTRYDGKPYVYEAVNWTHTESATTACLIVIVFTLIIHNILFCITFIKFLL---------------KESYSDIKKSVNSD 911
            DS W  +    F  YR+++ +  L ++ FDI  E  N++      WF+YATNW+F + + +  +LA  C+I +  PR +      +G I+L+Y I   + + V +VYWVAL N+  G+ S    ++KH++   + ++DL+I+ I + LAHV+YPI+ G+IY +F+ +++  GG    G   VY  +NW H ++A  +CL  ++F+ +IH +++   +I+  L               +E Y+D++  V ++
Sbjct:   44 DSQWLWMKPWLFTTYRVLLSLGTLVWICFDIPREIHNYFHDKTHIWFVYATNWSFGLLTLAFFLLALYCLI-YNCPRRKDATLILYGPIWLIYIIALDTIIVVSIVYWVALANSSLGVFSSIWGKLKHSLAACLAILDLLISSIPVRLAHVVYPILMGLIYGVFSYLFWLGGGEGPLGVGQVYPGLNWIHPKTAILSCLCALLFSFLIHILIYVTYYIRVKLSTCFGGRHAETVNQMEEGYNDLRDDVENE 293
[Close]
BLAST of Protein rolling stone vs. TrEMBL
Match: A0A4S2MEF6 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_000642 PE=4 SV=1)

HSP 1 Score: 102.449 bits (254), Expect = 2.688e-21
Identity = 82/253 (32.41%), Postives = 141/253 (55.73%), Query Frame = 3 
Query:  210 DSMWSRLNVKPFL--AYRIIIGIFGLAYVIFDITWETINFYKHNIGYWFIYATNWAFVIFSFSMAILAANCVIRFFKPRYEFNDNYCHG-IFLLYTITAVSTLTVDLVYWVAL-NAEKGLMSESEARIKHTIPTIIILVDLMINRIVLELAHXXXXXXXXXXXXXFNAIYYHSGGTRYDGKPYVYEAVNWTHTESATTACLIVIVFTLIIHNILFCITFIKFLL---------------KESYSDIKKSVNSD 911
            DS W  L +KP+L   YR+++ +  L ++ FDI  E  N++      WF+YATNW+F + + +  +LA  C+I +  PR        +G I+L+Y I   +   V +VYWVAL N+  G+ S    +IKH++   + ++DL+I+ I + LAH +YPI+ G+IY +F+ +++  GG    G   VY  +NW H ++A  +CL  I+ + +IH +++   +I+  L               +E Y+DI+  V ++
Sbjct:   44 DSQW--LWMKPWLFTTYRVLLSLGSLVWICFDIPREIHNYFHDKTHIWFVYATNWSFGLLTLAFFLLALYCLI-YNCPRRRDATLILYGPIWLIYIIALDTIFVVSIVYWVALANSNLGVFSSIWGKIKHSLAACLAVLDLLISSIPVRLAHAVYPILIGLIYAVFSYLFWLGGGEGPLGVGQVYPGLNWIHPKTAILSCLCAILLSFLIHILIYVTYYIRVKLSTCFDGRHAETVKQMEEGYNDIRGDVENE 293
[Close]
BLAST of Protein rolling stone vs. TrEMBL
Match: A0A183JDN5 (Uncharacterized protein OS=Schistosoma curassoni OX=6186 GN=SCUD_LOCUS798 PE=4 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 3.997e-20
Identity = 64/210 (30.48%), Postives = 119/210 (56.67%), Query Frame = 3 
Query:  237 KPFLAYRIIIGIFGLAYVIFDITWETINFYKHNIGYWFIYATNWAFVIFSFSMAILAANCVIRFFKPRYEFND-NYCHGIFLLYTITAVSTLTVDLVYWVALNA-EKGLMSESEARIKHTIPTIIILVDLMINRIVLELAHXXXXXXXXXXXXXFNAIYYHSGGTRYDGKPYVYEAVNWTHTESATTACLIVIVFTLIIHNILFCITFIK 860
            K F  YR+ + I  L ++I+DI  ETI  + H+   W  Y TNWAF+I +     LA  C+I  +  +Y  N+  Y   I+ LY +++   L V +++W +L+     + +  ++R+KH++ ++++++D  +  I + + HVIYP + GVIY +F  +++  GGT    K  +Y +++W+  +S   AC   ++  ++ H  L+ I FI+
Sbjct:    6 KYFTTYRVSLAILLLVWIIYDIVIETIKHFDHSEYSWITYGTNWAFLILTIFHISLAIYCLI--YNVKYSNNEPRYYQPIWFLYNLSSTCILVVSIIFWFSLSTIPLNVFASWQSRVKHSLTSVLVVIDTCLCAIPMRMIHVIYPCLLGVIYSLFTYLFWLLGGTGPYNKGQIYPSIDWSKPQSTILACFSALMSVILCHATLYIIYFIR 213
[Close]
BLAST of Protein rolling stone vs. TrEMBL
Match: A0A5J4NKD3 (Uncharacterized protein OS=Paragonimus westermani OX=34504 GN=DEA37_0003886 PE=4 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 8.130e-20
Identity = 72/246 (29.27%), Postives = 126/246 (51.22%), Query Frame = 3 
Query:  141 NLLLNQFNVEKCLPILSEKAAIF-DSMWSRLNVKPFLAYRIIIGIFGLAYVIFDITWETINFYKHNIGYWFIYATNWAFVIFSFSMAILAANCVIRFFKPRYEFNDNYCHGIFLLYTITAVSTLTVDLVYWVALNAEK-GLMSESEARIKHTIPTIIILVDLMINRIVLELAHXXXXXXXXXXXXXFNAIYYHSGGTRYDGKPYVYEAVNWTHTESATTACLIVIVFTLIIHNILFCITFIKFLLK 872
            N  + +F+  +CL     +   F +S W  +    F  YR+++ +  L ++ FDI  ET  ++ +    WF+YATNW F +      +L   C++     R   +      ++LLYT+     L V LV+WVAL++   G+ +    R+KH++  I+ ++DL I+ I   L HV YPI+ G++Y +F+  ++    T       VY  +NW H  +A  ACL V++ + ++H  L+   F++  L 
Sbjct:   21 NFFITEFS-WRCLGFNHSRPTDFTESQWVWMKPWLFTIYRVLLALSMLIWISFDIPRETHRYFGNRTYIWFVYATNWTFGLLVLVFFLLTVQCLVYNCPKRDNASVILYQPLWLLYTVAVNGILVVCLVFWVALSSSDLGVFASGWGRLKHSLSGILAILDLGISAIPFRLVHVTYPILAGILYSMFSYFFWLGDLTGPVAVGQVYPGLNWAHPRAAILACLCVLLVSFLVHICLYVTYFLRVQLS 265
[Close]
BLAST of Protein rolling stone vs. Ensembl Nematostella
Match: EDO35079 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SMI1])

HSP 1 Score: 61.6178 bits (148), Expect = 1.998e-11
Identity = 50/186 (26.88%), Postives = 94/186 (50.54%), Query Frame = 3 
Query:  345 WFIYATNWAFVIFSFSMAILAANCVIRFFKPRYEFNDNYCHGIFLLYTITAVSTLTVDLVYWVALNAEKGLMSESEARIKHTIPTIIILVDLMINRIVLELAHXXXXXXXXXXXXXFNAIYYHSGGTRYDGKPYVYEAVNWTHTE--SATTACLIVIVFTLIIHNILFCI-TFIKFLLKESYSDIK 893
            WFI+ TNW++ I +    + A N  +             C   ++++ I A     V  +YW   N   G   +S     H +  +++  D+M++ + + + HVIY ++CG  Y+ F  IY+ +GG  ++G  Y+Y A+++T +   +AT A L V++   + H +L+ +  F  +L+K S   I+
Sbjct:    9 WFIFLTNWSYTILNIHFILGALNTKLTTLPQNSRMFILACKVSWVVFNIAANVAPLVTFMYW-GFNYVPGQYVDSSNINAHALNCVLMFADVMLSNLPVRVLHVIYALVCGFAYVTFTVIYWAAGGVNHEGNSYIYSALDYTGSPGMAATMAVLFVLLAQPLSHLLLYGLYCFRDWLVKRSTRAIE 193
[Close]
BLAST of Protein rolling stone vs. Ensembl Nematostella
Match: EDO34937 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SMX4])

HSP 1 Score: 54.6842 bits (130), Expect = 6.881e-9
Identity = 54/224 (24.11%), Postives = 114/224 (50.89%), Query Frame = 3 
Query:  246 LAYRIIIGIFGLAYVIFDITWETINFYKHNIGYWFIYATNWAFVIFSFSMAILAANCVIRFFKPR---------YEFND--NYCHGI-FLLYTITAVSTLTVDLVYWVALNAEKGLMSESEARIKHTIPTIIILVDLMINRIVLELAHXXXXXXXXXXXXXFNAIYYHSGGTRYDGKPYVYEAVNWTHTESATTACLIVIVFTL--IIHNILFCITFIKFLLKE 875
            L +R ++  +   ++I+    E     K     WFIY TNW +++ +  +  + A+ V  +F  R         + F+D   Y H + ++L+ I A + + + ++YW  +      +  S     H + T+ ++++L+++++ + L H IYP++ G+ Y+IF  +Y+ +GGT  + KPY+Y  V++  +       L+  +  L  I+H++ F +  ++  L E
Sbjct:    2 LVFRFLVAAYNTGWLIYSGFHEANGKTK-----WFIYLTNWGYLLET--VYFIYASAVTLWFHIREKKNKDQSDFNFSDLMRYHHSLMWILFNIAADTAVLISIIYWGWMVGPIDGLDVST----HALNTVFMILELVLSKLPIRLLHCIYPLVFGLCYMIFTVVYWAAGGTNANNKPYIYSIVDYKDSPGMAVGILLGALLVLLPIVHSVFFGVYKLRCYLSE 214
[Close]
BLAST of Protein rolling stone vs. Ensembl Nematostella
Match: EDO34938 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SMX5])

HSP 1 Score: 53.1434 bits (126), Expect = 4.083e-8
Identity = 63/257 (24.51%), Postives = 112/257 (43.58%), Query Frame = 3 
Query:  246 LAYRIIIGIFGLAYVIFDITWETINFYKHNIGYWFIYATNWAFVI----FSFSMAILAANCVIRFFKPRYEFNDN-------------------------------------------YCHGIFLLYTITAVSTLTVDLVYW-VALNAEKGLMSESEARIKHTIPTIIILVDLMINRIVLELAHXXXXXXXXXXXXXFNAIYYHSGGTRYDGKPYVYEAVNWTHTESATTACLIVIVFTLIIHNILFCITFIKFLLK 872
            L YR++  I+  A++I      +    K     WFIY TNW+++I    F+ S A+ A      +F   Y+  D+                                           Y + ++LL+ + A S + V LVYW ++  + +G   +      H +  + +++DL ++ + + L  +IYP +  +IY+IF  +Y+ S GT  +G+PYVY++ ++  TE    A    I F LI   +   I F  + L+
Sbjct:   45 LIYRLVFAIYCFAWLIAGTQHPSNGQEK-----WFIYLTNWSYLILTLYFACSTALTAVYYRGEWFVGEYDVQDHDALKPRSRSRPGDPRDPSCDQSRERLGSHVSPLHVKVSEPMNWYHYPLWLLHIVAANSAVAVCLVYWMISYPSYQGYTPDGLDISTHLLNALFVVIDLCLSSLPVRLLQIIYPFVYFIIYLIFLVLYWASDGTNANGQPYVYKSTDF--TERPARAAGFAIGFPLIFQMLAQVILFSLYKLR 294
[Close]
BLAST of Protein rolling stone vs. Ensembl Nematostella
Match: EDO45426 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RT93])

HSP 1 Score: 49.2914 bits (116), Expect = 7.619e-7
Identity = 57/229 (24.89%), Postives = 96/229 (41.92%), Query Frame = 3 
Query:  204 IFDSMWSRLNVKPFLAYRIIIGIFGLAYVIFDITWETINFYKHNIGYWFIYATNWAFVIFSF-----------------------------SMAILAANCVIRFFKPRYEFND--NYCHGI-FLLYTITAVSTLTVDLVYWVALNAEKGLMSESEARIKHTIPTIIILVDLMINRIVLELAHXXXXXXXXXXXXXFNAIYYHSGGTRYDGKPYVYEAVNWTHTESATTA 794
            I +S W  L V  F+ +R++I I      ++++          + G +FI+ TNW+F + +                               + +   N      +P  E  D     H I +LLY I A  TL + + YW  +N      +      KH    I  ++D  ++RI + + H +Y +I   +Y+IF  IY+  GGT   G PY+Y  +++   +S TTA
Sbjct:   20 ITESAWLPLPV--FVGFRVVISILCCVIWLYNVI---------SKGKFFIFYTNWSFTVATIYFIVSSVVSMMQYLAKRKYPEKPGASKAVDVEMQPVNASPNLPRPDNEHQDAIGAHHMITWLLYNIAANMTLFLTIGYWTMINDPDKNPNTFVGVFKHLFVAIFCVIDTFMSRIPVRIYHFVYTLIATAVYLIFTIIYWSVGGTDLHGNPYIYTVLDY---DSMTTA 234
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BLAST of Protein rolling stone vs. Planmine SMEST
Match: SMESG000042952.1 (SMESG000042952.1)

HSP 1 Score: 292.352 bits (747), Expect = 1.059e-100
Identity = 142/142 (100.00%), Postives = 142/142 (100.00%), Query Frame = 3 
Query:  129 MATSNLLLNQFNVEKCLPILSEKAAIFDSMWSRLNVKPFLAYRIIIGIFGLAYVIFDITWETINFYKHNIGYWFIYATNWAFVIFSFSMAILAANCVIRFFKPRYEFNDNYCHGIFLLYTITAVSTLTVDLVYWVALNAEKG 554
            MATSNLLLNQFNVEKCLPILSEKAAIFDSMWSRLNVKPFLAYRIIIGIFGLAYVIFDITWETINFYKHNIGYWFIYATNWAFVIFSFSMAILAANCVIRFFKPRYEFNDNYCHGIFLLYTITAVSTLTVDLVYWVALNAEKG
Sbjct:    1 MATSNLLLNQFNVEKCLPILSEKAAIFDSMWSRLNVKPFLAYRIIIGIFGLAYVIFDITWETINFYKHNIGYWFIYATNWAFVIFSFSMAILAANCVIRFFKPRYEFNDNYCHGIFLLYTITAVSTLTVDLVYWVALNAEKG 142
[Close]
BLAST of Protein rolling stone vs. Planmine SMEST
Match: SMESG000027691.1 (SMESG000027691.1)

HSP 1 Score: 210.305 bits (534), Expect = 6.816e-69
Identity = 102/107 (95.33%), Postives = 103/107 (96.26%), Query Frame = 3 
Query:  129 MATSNLLLNQFNVEKCLPILSEKAAIFDSMWSRLNVKPFLAYRIIIGIFGLAYVIFDITWETINFYKHNIGYWFIYATNWAFVIFSFSMAILAANCVIRFFKPRYEF 449
            MA S+LLLNQFNVEKCLPILSEKAAIFDSMWSRLNVKPFLAYRIIIGIFGLAYVIFDITWETINFYKHNIGYWFIYATNWAFVIFSFSMAILAANCVIRFFKP   F
Sbjct:    1 MANSSLLLNQFNVEKCLPILSEKAAIFDSMWSRLNVKPFLAYRIIIGIFGLAYVIFDITWETINFYKHNIGYWFIYATNWAFVIFSFSMAILAANCVIRFFKPSLRF 107
[Close]
BLAST of Protein rolling stone vs. Planmine SMEST
Match: SMESG000077396.1 (SMESG000077396.1)

HSP 1 Score: 103.219 bits (256), Expect = 1.880e-25
Identity = 73/218 (33.49%), Postives = 119/218 (54.59%), Query Frame = 3 
Query:  210 DSMWSRLNVKPFLAYRIIIGIFGLAYVIFDITWETINFYKHNIGYWFIYATNWAFVIFSFSMAILAANCVIRFFKPRYEFNDNYCHGI------FLLYTITAVSTLTVDLVYWVAL-NAEKGLMSESEARIKHTIPTIIILVDLMINRIVLELAHXXXXXXXXXXXXXFNAIYYHSGGTRYDGKPYVYEAVNWTHTESATTACLIVIVFTLIIHNILF 842
            +S W  ++ K F  YR+I+ I  + + I D   E +N Y+    +W + ATNWAF      MA++ A+  + F       +D     +      ++ Y I   S LT  ++Y  AL N+E+ + + ++++IKH +  ++IL DL I  I + L H IYP + G++Y I   IYY+ GG   +GKPY+YEA+ W +  SA  A + VI+ +++I   LF
Sbjct:   21 ESQWDFISPKLFTCYRVIMAIGMVLWAISDFASEWLNSYRGYPYFWLLMATNWAF------MAMVIAHIALAFVNILTNCHDVRSQNVVGYKMCWIFYNIACNSVLTTSIIYLFALVNSEQEIFNTTQSKIKHCLNALLILFDLAICSIPIRLFHFIYPFMLGILYTIMLVIYYYGGGIGPNGKPYIYEALYWKYPGSAIVAVVCVIIMSILIQLFLF 232
The following BLAST results are available for this feature:
BLAST of Protein rolling stone vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Protein rolling stone vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Protein rolling stone vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CG437782.683e-1328.30gene:FBgn0264308 transcript:FBtr0331503[more]
CG131014.245e-1026.82gene:FBgn0032084 transcript:FBtr0079784[more]
CG131014.245e-1026.82gene:FBgn0032084 transcript:FBtr0079783[more]
CG437785.397e-1028.30gene:FBgn0264308 transcript:FBtr0331505[more]
rost1.420e-822.92gene:FBgn0011705 transcript:FBtr0331454[more]
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BLAST of Protein rolling stone vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Protein rolling stone vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Protein rolling stone vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Protein rolling stone vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 1
Match NameE-valueIdentityDescription
sp|O44252|ROST_DROME1.423e-722.92Protein rolling stone OS=Drosophila melanogaster O... [more]
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BLAST of Protein rolling stone vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
G7YA022.333e-2132.02Protein rolling stone OS=Clonorchis sinensis OX=79... [more]
A0A074ZRW12.581e-2131.08Uncharacterized protein OS=Opisthorchis viverrini ... [more]
A0A4S2MEF62.688e-2132.41Uncharacterized protein OS=Opisthorchis felineus O... [more]
A0A183JDN53.997e-2030.48Uncharacterized protein OS=Schistosoma curassoni O... [more]
A0A5J4NKD38.130e-2029.27Uncharacterized protein OS=Paragonimus westermani ... [more]
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BLAST of Protein rolling stone vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Protein rolling stone vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Protein rolling stone vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Protein rolling stone vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 4
Match NameE-valueIdentityDescription
EDO350791.998e-1126.88Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO349376.881e-924.11Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO349384.083e-824.51Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO454267.619e-724.89Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Protein rolling stone vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Protein rolling stone vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000042952.11.059e-100100.00SMESG000042952.1[more]
SMESG000027691.16.816e-6995.33SMESG000027691.1[more]
SMESG000077396.11.880e-2533.49SMESG000077396.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30020068 ID=SMED30020068|Name=Protein rolling stone|organism=Schmidtea mediterranea sexual|type=transcript|length=948bp
TTGAATCATTTTGTGATACACATGACACTTGAGATACAAATCATATCAAT
GACCTTTGCCATTTATTAATTGCATTAGGATTTGATTTCACTTGATTACT
TTTACATTTGTTTGATCACATCATTTATATGGCAACTAGCAATCTTCTAC
TGAATCAATTTAATGTGGAAAAATGCTTACCAATATTGTCCGAAAAGGCT
GCAATATTCGATTCAATGTGGAGTAGACTCAATGTGAAACCATTTTTAGC
GTATAGAATCATAATTGGTATATTTGGACTGGCATATGTTATATTTGACA
TCACATGGGAAACAATTAATTTCTATAAGCATAACATTGGATATTGGTTC
ATTTATGCTACAAATTGGGCGTTCGTCATCTTTTCTTTTTCAATGGCTAT
CTTAGCTGCCAATTGCGTAATACGATTTTTCAAACCAAGATATGAATTTA
ATGACAATTATTGTCATGGTATATTTCTGTTGTATACGATCACGGCTGTG
AGCACTCTGACTGTCGATTTAGTTTATTGGGTTGCACTTAATGCCGAGAA
AGGACTTATGAGCGAGTCCGAAGCCAGGATCAAGCATACAATTCCTACAA
TTATCATCTTAGTGGATCTTATGATTAACAGAATAGTTTTAGAACTGGCT
CATGTAATCTATCCCATAATTTGCGGAGTTATATATATTATTTTCAATGC
TATTTATTATCATTCTGGTGGCACGCGTTATGATGGTAAGCCATACGTTT
ACGAAGCTGTCAATTGGACTCATACTGAATCAGCAACTACTGCCTGCTTA
ATTGTAATCGTATTTACTTTGATCATCCACAATATTCTTTTCTGCATTAC
TTTCATCAAATTTCTTCTCAAAGAATCCTATTCAGATATCAAAAAATCAG
TAAATTCAGATTCAGTTTGTTTAGTAAAATAAAACAAATAAACTTTTG
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protein sequence of SMED30020068-orf-1

>SMED30020068-orf-1 ID=SMED30020068-orf-1|Name=SMED30020068-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=268bp
MATSNLLLNQFNVEKCLPILSEKAAIFDSMWSRLNVKPFLAYRIIIGIFG
LAYVIFDITWETINFYKHNIGYWFIYATNWAFVIFSFSMAILAANCVIRF
FKPRYEFNDNYCHGIFLLYTITAVSTLTVDLVYWVALNAEKGLMSESEAR
IKHTIPTIIILVDLMINRIVLELAHVIYPIICGVIYIIFNAIYYHSGGTR
YDGKPYVYEAVNWTHTESATTACLIVIVFTLIIHNILFCITFIKFLLKES
YSDIKKSVNSDSVCLVK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000043dorsal ventral margin of the whole animal
PLANA:0000070intestinal phagocyte
Vocabulary: INTERPRO
TermDefinition
IPR039860Rost
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR12242UNCHARACTERIZEDcoord: 21..247
NoneNo IPR availableTMHMMTMhelixcoord: 73..95
NoneNo IPR availableTMHMMTMhelixcoord: 224..246
NoneNo IPR availableTMHMMTMhelixcoord: 172..194
NoneNo IPR availableTMHMMTMhelixcoord: 39..58
NoneNo IPR availableTMHMMTMhelixcoord: 115..137
IPR039860Protein rolling stonePANTHERPTHR12242:SF1LD06593P-RELATEDcoord: 21..247