MRP-S23 domain-containing protein

Overview
NameMRP-S23 domain-containing protein
Smed IDSMED30020040
Length (bp)515
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of MRP-S23 domain-containing protein (SMED30020040) t-SNE clustered cells

Violin plots show distribution of expression levels for MRP-S23 domain-containing protein (SMED30020040) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of MRP-S23 domain-containing protein (SMED30020040) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for MRP-S23 domain-containing protein (SMED30020040) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30020040

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30020040SMESG000056643.1 SmedASXL_019322SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
Smed sexual biotypeSMED30020040SMESG000056643.1 SMESG000020955.1 Contig38434newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30020040SMESG000056643.1 SMESG000020955.1 Contig38434uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of MRP-S23 domain-containing protein vs. Ensembl Human
Match: MRPS23 (mitochondrial ribosomal protein S23 [Source:HGNC Symbol;Acc:HGNC:14509])

HSP 1 Score: 50.8322 bits (120), Expect = 5.885e-8
Identity = 40/118 (33.90%), Postives = 55/118 (46.61%), Query Frame = 1 
Query:  121 MAGSRKEKFIPLIERATGLLKNKVIKDVDTPIWYDVYKAFPPEVSPNFNRPIPNQNVPK-----ILYPEDVHRAFFIGHPLYRNAIFNLFHIENKDNYSYKFLEEVKKLKNEFEDINE 459
            MAGSR E    +  R   L++  V+K+   P+W+DVY AFPP   P F RP       K     I Y ED  RA F          F+LF+  N  +   +F+E+  +L+   E   E
Sbjct:    1 MAGSRLETVGSIFSRTRDLVRAGVLKE--KPLWFDVYDAFPPLREPVFQRPRVRYGKAKAPIQDIWYHEDRIRAKFYSVYGSGQRAFDLFN-PNFKSTCQRFVEKYTELQKLGETDEE 115
[Close]
BLAST of MRP-S23 domain-containing protein vs. Ensembl Human
Match: MRPS23 (mitochondrial ribosomal protein S23 [Source:HGNC Symbol;Acc:HGNC:14509])

HSP 1 Score: 49.6766 bits (117), Expect = 2.683e-7
Identity = 40/118 (33.90%), Postives = 55/118 (46.61%), Query Frame = 1 
Query:  121 MAGSRKEKFIPLIERATGLLKNKVIKDVDTPIWYDVYKAFPPEVSPNFNRPIPNQNVPK-----ILYPEDVHRAFFIGHPLYRNAIFNLFHIENKDNYSYKFLEEVKKLKNEFEDINE 459
            MAGSR E    +  R   L++  V+K+   P+W+DVY AFPP   P F RP       K     I Y ED  RA F          F+LF+  N  +   +F+E+  +L+   E   E
Sbjct:    1 MAGSRLETVGSIFSRTRDLVRAGVLKE--KPLWFDVYDAFPPLREPVFQRPRVRYGKAKAPIQDIWYHEDRIRAKFYSVYGSGQRAFDLFN-PNFKSTCQRFVEKYTELQKLGETDEE 115
[Close]
BLAST of MRP-S23 domain-containing protein vs. Ensembl Celegans
Match: mrps-23 (Probable 28S ribosomal protein S23, mitochondrial [Source:UniProtKB/Swiss-Prot;Acc:P34748])

HSP 1 Score: 53.9138 bits (128), Expect = 1.405e-9
Identity = 34/106 (32.08%), Postives = 56/106 (52.83%), Query Frame = 1 
Query:  130 SRKEKFIPLIERATGLLKNKVIKDVDTPIWYDVYKAFPPEVSPNFNRPIPNQNVP--KILYPEDVHRAFFIGHPLYRNAIFNLFHIENKDNYSYKFLEEVKKLKNE 441
            +R E+   +  R TGL++   +   D P+WYDVY + PP   P++N  +   + P   I Y EDV RA F  +  YR+    +    ++ + S +F+ E K +K+E
Sbjct:    6 TRAERSGNIFSRVTGLIRAGQLNWADRPLWYDVYVSSPPLTPPDWNVKLAKYDEPIRSIFYEEDVLRAKF--YKTYRSTA-GIQVDSSRTSVSQQFINEYKLVKSE 108
[Close]
BLAST of MRP-S23 domain-containing protein vs. Ensembl Celegans
Match: mrps-23 (Probable 28S ribosomal protein S23, mitochondrial [Source:UniProtKB/Swiss-Prot;Acc:P34748])

HSP 1 Score: 53.9138 bits (128), Expect = 1.405e-9
Identity = 34/106 (32.08%), Postives = 56/106 (52.83%), Query Frame = 1 
Query:  130 SRKEKFIPLIERATGLLKNKVIKDVDTPIWYDVYKAFPPEVSPNFNRPIPNQNVP--KILYPEDVHRAFFIGHPLYRNAIFNLFHIENKDNYSYKFLEEVKKLKNE 441
            +R E+   +  R TGL++   +   D P+WYDVY + PP   P++N  +   + P   I Y EDV RA F  +  YR+    +    ++ + S +F+ E K +K+E
Sbjct:    6 TRAERSGNIFSRVTGLIRAGQLNWADRPLWYDVYVSSPPLTPPDWNVKLAKYDEPIRSIFYEEDVLRAKF--YKTYRSTA-GIQVDSSRTSVSQQFINEYKLVKSE 108
[Close]
BLAST of MRP-S23 domain-containing protein vs. Ensembl Fly
Match: mRpS23 (gene:FBgn0260407 transcript:FBtr0080566)

HSP 1 Score: 74.7146 bits (182), Expect = 6.176e-17
Identity = 36/69 (52.17%), Postives = 43/69 (62.32%), Query Frame = 1 
Query:  121 MAGSRKEKFIPLIERATGLLKNKVIKDVDTPIWYDVYKAFPPEVSPNFNRPIPNQNVPKILYPEDVHRA 327
            MA SR EK   +  R  GLL+   +K  D PIWYDVY AFPP++ P F+RP P   V +I Y EDV RA
Sbjct:    1 MAQSRLEKIGTIFTRVQGLLRGGAMKTEDKPIWYDVYAAFPPKLEPRFDRPAPEIPVRQIFYAEDVVRA 69
[Close]
BLAST of MRP-S23 domain-containing protein vs. Ensembl Zebrafish
Match: mrps23 (mitochondrial ribosomal protein S23 [Source:ZFIN;Acc:ZDB-GENE-040801-134])

HSP 1 Score: 62.3882 bits (150), Expect = 3.581e-12
Identity = 35/76 (46.05%), Postives = 46/76 (60.53%), Query Frame = 1 
Query:  121 MAGSRKEKFIPLIERATGLLKNKVIKDVDTPIWYDVYKAFPPEVSPNFNRP----IPNQN-VPKILYPEDVHRAFF 333
            MAGSR EKF  +  R   LL+  V+K  + PIWYDVY AFPP+  P + +P    +P  + VP++ Y ED  RA F
Sbjct:    1 MAGSRLEKFGTVFTRVRDLLRAGVLKPEEKPIWYDVYAAFPPKKEPLYQKPRRPRVPAADPVPQLFYKEDEIRAKF 76
[Close]
BLAST of MRP-S23 domain-containing protein vs. Ensembl Zebrafish
Match: mrps23 (mitochondrial ribosomal protein S23 [Source:ZFIN;Acc:ZDB-GENE-040801-134])

HSP 1 Score: 62.3882 bits (150), Expect = 3.673e-12
Identity = 35/76 (46.05%), Postives = 46/76 (60.53%), Query Frame = 1 
Query:  121 MAGSRKEKFIPLIERATGLLKNKVIKDVDTPIWYDVYKAFPPEVSPNFNRP----IPNQN-VPKILYPEDVHRAFF 333
            MAGSR EKF  +  R   LL+  V+K  + PIWYDVY AFPP+  P + +P    +P  + VP++ Y ED  RA F
Sbjct:    1 MAGSRLEKFGTVFTRVRDLLRAGVLKPEEKPIWYDVYAAFPPKKEPLYQKPRRPRVPAADPVPQLFYKEDEIRAKF 76
[Close]
BLAST of MRP-S23 domain-containing protein vs. Ensembl Xenopus
Match: casz1 (castor zinc finger 1 [Source:Xenbase;Acc:XB-GENE-957461])

HSP 1 Score: 67.781 bits (164), Expect = 5.329e-14
Identity = 34/76 (44.74%), Postives = 45/76 (59.21%), Query Frame = 1 
Query:  121 MAGSRKEKFIPLIERATGLLKNKVIKDVDTPIWYDVYKAFPPEVSPNFNRPIPNQN-----VPKILYPEDVHRAFF 333
            MAGSR EK   +  R   LL+  +IK  + P+WYDVY AFPP+  P + +P+  +      VP ILY ED+ RA F
Sbjct:    1 MAGSRLEKLGTVFSRVRDLLRAGIIKQNEKPVWYDVYAAFPPKREPLYEKPLRRKQITSDIVPSILYKEDIIRAKF 76
[Close]
BLAST of MRP-S23 domain-containing protein vs. Ensembl Mouse
Match: Mrps23 (mitochondrial ribosomal protein S23 [Source:MGI Symbol;Acc:MGI:1928138])

HSP 1 Score: 59.6918 bits (143), Expect = 3.140e-11
Identity = 41/118 (34.75%), Postives = 58/118 (49.15%), Query Frame = 1 
Query:  121 MAGSRKEKFIPLIERATGLLKNKVIKDVDTPIWYDVYKAFPPEVSPNFNRPI-----PNQNVPKILYPEDVHRAFFIGHPLYRNAIFNLFHIENKDNYSYKFLEEVKKLKNEFEDINE 459
            MAGSR E    +  R   L++  V+K+   P+WYD+YKAFPP   P F RP         ++  I Y ED  RA F          F+LF+  N  +   +F+E+  +L+N  E   E
Sbjct:    1 MAGSRLETVGSVFSRTRDLMRAGVLKE--KPLWYDIYKAFPPLREPVFRRPRLRYGKAKADIQDIFYQEDQIRAKFFATYGSGQKAFDLFN-PNFKSTCQRFVEKYTELQNLGETDEE 115
[Close]
BLAST of MRP-S23 domain-containing protein vs. Ensembl Mouse
Match: Mrps23 (mitochondrial ribosomal protein S23 [Source:MGI Symbol;Acc:MGI:1928138])

HSP 1 Score: 59.6918 bits (143), Expect = 3.140e-11
Identity = 41/118 (34.75%), Postives = 58/118 (49.15%), Query Frame = 1 
Query:  121 MAGSRKEKFIPLIERATGLLKNKVIKDVDTPIWYDVYKAFPPEVSPNFNRPI-----PNQNVPKILYPEDVHRAFFIGHPLYRNAIFNLFHIENKDNYSYKFLEEVKKLKNEFEDINE 459
            MAGSR E    +  R   L++  V+K+   P+WYD+YKAFPP   P F RP         ++  I Y ED  RA F          F+LF+  N  +   +F+E+  +L+N  E   E
Sbjct:    1 MAGSRLETVGSVFSRTRDLMRAGVLKE--KPLWYDIYKAFPPLREPVFRRPRLRYGKAKADIQDIFYQEDQIRAKFFATYGSGQKAFDLFN-PNFKSTCQRFVEKYTELQNLGETDEE 115
[Close]
BLAST of MRP-S23 domain-containing protein vs. Ensembl Mouse
Match: Mrps23 (mitochondrial ribosomal protein S23 [Source:MGI Symbol;Acc:MGI:1928138])

HSP 1 Score: 56.9954 bits (136), Expect = 8.077e-11
Identity = 34/91 (37.36%), Postives = 45/91 (49.45%), Query Frame = 1 
Query:  121 MAGSRKEKFIPLIERATGLLKNKVIKDVDTPIWYDVYKAFPPEVSPNFNRPI-----PNQNVPKILYPEDVHRAFFIGHPLYRNAIFNLFH 378
            MAGSR E    +  R   L++  V+K+   P+WYD+YKAFPP   P F RP         ++  I Y ED  RA F          F+LF+
Sbjct:    1 MAGSRLETVGSVFSRTRDLMRAGVLKE--KPLWYDIYKAFPPLREPVFRRPRLRYGKAKADIQDIFYQEDQIRAKFFATYGSGQKAFDLFN 89
[Close]
BLAST of MRP-S23 domain-containing protein vs. Ensembl Mouse
Match: Mrps23 (mitochondrial ribosomal protein S23 [Source:MGI Symbol;Acc:MGI:1928138])

HSP 1 Score: 56.9954 bits (136), Expect = 8.077e-11
Identity = 34/91 (37.36%), Postives = 45/91 (49.45%), Query Frame = 1 
Query:  121 MAGSRKEKFIPLIERATGLLKNKVIKDVDTPIWYDVYKAFPPEVSPNFNRPI-----PNQNVPKILYPEDVHRAFFIGHPLYRNAIFNLFH 378
            MAGSR E    +  R   L++  V+K+   P+WYD+YKAFPP   P F RP         ++  I Y ED  RA F          F+LF+
Sbjct:    1 MAGSRLETVGSVFSRTRDLMRAGVLKE--KPLWYDIYKAFPPLREPVFRRPRLRYGKAKADIQDIFYQEDQIRAKFFATYGSGQKAFDLFN 89
[Close]
BLAST of MRP-S23 domain-containing protein vs. Ensembl Mouse
Match: Mrps23 (mitochondrial ribosomal protein S23 [Source:MGI Symbol;Acc:MGI:1928138])

HSP 1 Score: 48.9062 bits (115), Expect = 1.219e-7
Identity = 35/102 (34.31%), Postives = 48/102 (47.06%), Query Frame = 1 
Query:  169 TGLLKNKVIKDVDTPIWYDVYKAFPPEVSPNFNRPI-----PNQNVPKILYPEDVHRAFFIGHPLYRNAIFNLFHIENKDNYSYKFLEEVKKLKNEFEDINE 459
             G+LK K       P+WYD+YKAFPP   P F RP         ++  I Y ED  RA F          F+LF+  N  +   +F+E+  +L+N  E   E
Sbjct:    3 AGVLKEK-------PLWYDIYKAFPPLREPVFRRPRLRYGKAKADIQDIFYQEDQIRAKFFATYGSGQKAFDLFN-PNFKSTCQRFVEKYTELQNLGETDEE 96
[Close]
BLAST of MRP-S23 domain-containing protein vs. UniProt/SwissProt
Match: sp|Q8VE22|RT23_MOUSE (28S ribosomal protein S23, mitochondrial OS=Mus musculus OX=10090 GN=Mrps23 PE=1 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 2.197e-10
Identity = 41/118 (34.75%), Postives = 58/118 (49.15%), Query Frame = 1 
Query:  121 MAGSRKEKFIPLIERATGLLKNKVIKDVDTPIWYDVYKAFPPEVSPNFNRPI-----PNQNVPKILYPEDVHRAFFIGHPLYRNAIFNLFHIENKDNYSYKFLEEVKKLKNEFEDINE 459
            MAGSR E    +  R   L++  V+K+   P+WYD+YKAFPP   P F RP         ++  I Y ED  RA F          F+LF+  N  +   +F+E+  +L+N  E   E
Sbjct:    1 MAGSRLETVGSVFSRTRDLMRAGVLKE--KPLWYDIYKAFPPLREPVFRRPRLRYGKAKADIQDIFYQEDQIRAKFFATYGSGQKAFDLFN-PNFKSTCQRFVEKYTELQNLGETDEE 115
[Close]
BLAST of MRP-S23 domain-containing protein vs. UniProt/SwissProt
Match: sp|P34748|RT23_CAEEL (Probable 28S ribosomal protein S23, mitochondrial OS=Caenorhabditis elegans OX=6239 GN=mrps-23 PE=3 SV=1)

HSP 1 Score: 53.9138 bits (128), Expect = 1.787e-8
Identity = 34/106 (32.08%), Postives = 56/106 (52.83%), Query Frame = 1 
Query:  130 SRKEKFIPLIERATGLLKNKVIKDVDTPIWYDVYKAFPPEVSPNFNRPIPNQNVP--KILYPEDVHRAFFIGHPLYRNAIFNLFHIENKDNYSYKFLEEVKKLKNE 441
            +R E+   +  R TGL++   +   D P+WYDVY + PP   P++N  +   + P   I Y EDV RA F  +  YR+    +    ++ + S +F+ E K +K+E
Sbjct:    6 TRAERSGNIFSRVTGLIRAGQLNWADRPLWYDVYVSSPPLTPPDWNVKLAKYDEPIRSIFYEEDVLRAKF--YKTYRSTA-GIQVDSSRTSVSQQFINEYKLVKSE 108
[Close]
BLAST of MRP-S23 domain-containing protein vs. UniProt/SwissProt
Match: sp|Q9Y3D9|RT23_HUMAN (28S ribosomal protein S23, mitochondrial OS=Homo sapiens OX=9606 GN=MRPS23 PE=1 SV=2)

HSP 1 Score: 49.6766 bits (117), Expect = 1.288e-6
Identity = 40/118 (33.90%), Postives = 55/118 (46.61%), Query Frame = 1 
Query:  121 MAGSRKEKFIPLIERATGLLKNKVIKDVDTPIWYDVYKAFPPEVSPNFNRPIPNQNVPK-----ILYPEDVHRAFFIGHPLYRNAIFNLFHIENKDNYSYKFLEEVKKLKNEFEDINE 459
            MAGSR E    +  R   L++  V+K+   P+W+DVY AFPP   P F RP       K     I Y ED  RA F          F+LF+  N  +   +F+E+  +L+   E   E
Sbjct:    1 MAGSRLETVGSIFSRTRDLVRAGVLKE--KPLWFDVYDAFPPLREPVFQRPRVRYGKAKAPIQDIWYHEDRIRAKFYSVYGSGQRAFDLFN-PNFKSTCQRFVEKYTELQKLGETDEE 115
[Close]
BLAST of MRP-S23 domain-containing protein vs. UniProt/SwissProt
Match: sp|Q2NL27|RT23_BOVIN (28S ribosomal protein S23, mitochondrial OS=Bos taurus OX=9913 GN=MRPS23 PE=1 SV=1)

HSP 1 Score: 49.6766 bits (117), Expect = 1.452e-6
Identity = 42/121 (34.71%), Postives = 58/121 (47.93%), Query Frame = 1 
Query:  121 MAGSRKEKFIPLIERATGLLKNKVIKDVDTPIWYDVYKAFPPEVSPNFNRP--------IPNQNVPKILYPEDVHRAFFIGHPLYRNAIFNLFHIENKDNYSYKFLE---EVKKLKNEFED 450
            MAGSR E    +  R   L++  V+K+   P+W+DVY AFPP   P F RP         P Q+   I Y ED  RA F          F+LF+  N  +   +F+E   E++KL    E+
Sbjct:    1 MAGSRLETVGSIFTRTRDLIRAGVLKE--KPLWFDVYNAFPPLREPVFRRPRLRYGKAKSPTQD---IYYHEDQIRAKFYAAYGSGPKAFDLFN-PNFKSTCQRFVEKYIELQKLGETDEE 115
[Close]
BLAST of MRP-S23 domain-containing protein vs. TrEMBL
Match: A0A430QUH6 (Small subunit ribosomal protein S23 OS=Schistosoma bovis OX=6184 GN=DC041_0007144 PE=4 SV=1)

HSP 1 Score: 97.4413 bits (241), Expect = 9.576e-23
Identity = 46/113 (40.71%), Postives = 69/113 (61.06%), Query Frame = 1 
Query:  121 MAGSRKEKFIPLIERATGLLKNKVIKDVDTPIWYDVYKAFPPEVSPNFNRPIPNQNVPKILYPEDVHR-AFFIGHPLYRNAIFNLFHIENKDNYSYKFLEEVKKLKNEFEDIN 456
            M GSRKE+F  + +R  GL++   +   D P+WYDVYKAFPP + P+++RP P   V  I+YPED  R AFF GH  +R    N+F + +  +   K L++ + L+  + D+N
Sbjct:    1 MLGSRKERFASIFKRVKGLIEKGALDYEDRPLWYDVYKAFPPRIDPSYDRPCPTTTVQNIIYPEDCERAAFFKGH--HRLETLNMFKLVDNRSTLSKLLKKCRNLRELYPDLN 111
[Close]
BLAST of MRP-S23 domain-containing protein vs. TrEMBL
Match: A0A183LPI2 (MRP-S23 domain-containing protein OS=Schistosoma margrebowiei OX=48269 GN=SMRZ_LOCUS5705 PE=4 SV=1)

HSP 1 Score: 96.6709 bits (239), Expect = 1.811e-22
Identity = 47/113 (41.59%), Postives = 68/113 (60.18%), Query Frame = 1 
Query:  121 MAGSRKEKFIPLIERATGLLKNKVIKDVDTPIWYDVYKAFPPEVSPNFNRPIPNQNVPKILYPEDVHR-AFFIGHPLYRNAIFNLFHIENKDNYSYKFLEEVKKLKNEFEDIN 456
            M GSRKE+F  + +R  G+++   +   D P+WYDVYKAFPP V P+++RP P   V  I+YPED  R AFF GH  +R    N+F + +  +   K L++ K L+    D+N
Sbjct:    1 MLGSRKERFASIFKRVKGMIEKGALDYEDRPLWYDVYKAFPPRVDPSYDRPCPTTTVQNIIYPEDCERAAFFKGH--HRLETLNMFKLVDNRSTLSKLLKKCKNLRELHPDLN 111
[Close]
BLAST of MRP-S23 domain-containing protein vs. TrEMBL
Match: A0A183P5S7 (MRP-S23 domain-containing protein OS=Schistosoma mattheei OX=31246 GN=SMTD_LOCUS9713 PE=4 SV=1)

HSP 1 Score: 95.9005 bits (237), Expect = 3.898e-22
Identity = 46/113 (40.71%), Postives = 68/113 (60.18%), Query Frame = 1 
Query:  121 MAGSRKEKFIPLIERATGLLKNKVIKDVDTPIWYDVYKAFPPEVSPNFNRPIPNQNVPKILYPEDVHR-AFFIGHPLYRNAIFNLFHIENKDNYSYKFLEEVKKLKNEFEDIN 456
            M GSRKE+F  + +R  G+++   +   D P+WYDVYKAFPP V P+++RP P   V  I+YPED  R AFF GH  +R    N+F + +  +   K L++ + L+    D+N
Sbjct:    1 MLGSRKERFASIFKRVKGMIEKGALDYEDRPLWYDVYKAFPPRVDPSYDRPCPTTTVQNIIYPEDCERAAFFKGH--HRLETLNMFKLVDNRSTLSKLLKKCRNLRELHPDLN 111
[Close]
BLAST of MRP-S23 domain-containing protein vs. TrEMBL
Match: A0A183QVY7 (MRP-S23 domain-containing protein OS=Schistosoma rodhaini OX=6188 PE=4 SV=1)

HSP 1 Score: 95.1301 bits (235), Expect = 7.612e-22
Identity = 45/113 (39.82%), Postives = 68/113 (60.18%), Query Frame = 1 
Query:  121 MAGSRKEKFIPLIERATGLLKNKVIKDVDTPIWYDVYKAFPPEVSPNFNRPIPNQNVPKILYPEDVHR-AFFIGHPLYRNAIFNLFHIENKDNYSYKFLEEVKKLKNEFEDIN 456
            M GSR+E+F  + +R  G+++   +   D P+WYDVYKAFPP V P+++RP P   V  I+YPED  R AFF G   +R    N+F++ +  +   K L E +KL+    D++
Sbjct:    1 MLGSRRERFASIFKRVKGMIEKGALDYEDRPLWYDVYKAFPPRVDPSYDRPCPTTTVQNIIYPEDCERAAFFKGQ--HRLETLNMFNLVDNRSTLSKLLRECRKLREVHPDLS 111
[Close]
BLAST of MRP-S23 domain-containing protein vs. TrEMBL
Match: G4V6P8 (MRP-S23 domain-containing protein OS=Schistosoma mansoni OX=6183 GN=Smp_068080 PE=4 SV=1)

HSP 1 Score: 93.5893 bits (231), Expect = 3.448e-21
Identity = 44/113 (38.94%), Postives = 68/113 (60.18%), Query Frame = 1 
Query:  121 MAGSRKEKFIPLIERATGLLKNKVIKDVDTPIWYDVYKAFPPEVSPNFNRPIPNQNVPKILYPEDVHR-AFFIGHPLYRNAIFNLFHIENKDNYSYKFLEEVKKLKNEFEDIN 456
            M GSR+E+F  + +R  G+++   +   D P+WYDVYKAFPP V P+++RP P   V  I+YPED  R AFF G   +R    N+F++ +  +   K L + +KL+    D++
Sbjct:    1 MLGSRRERFASIFKRVKGMIEKGALDYEDRPLWYDVYKAFPPRVDPSYDRPCPTTTVQNIIYPEDCERAAFFKGQ--HRLETLNMFNLVDNRSTLSKLLRKCRKLREVHPDLS 111
[Close]
BLAST of MRP-S23 domain-containing protein vs. Ensembl Cavefish
Match: mrps23 (mitochondrial ribosomal protein S23 [Source:NCBI gene;Acc:111194926])

HSP 1 Score: 59.3066 bits (142), Expect = 3.653e-11
Identity = 44/131 (33.59%), Postives = 65/131 (49.62%), Query Frame = 1 
Query:  121 MAGSRKEKFIPLIERATGLLKNKVIKDVDTPIWYDVYKAFPPEVSP----NFNRPIPNQN---VPKILYPEDVHRAFFIGHPLYRNAIFNLFHIENK--DNYSYKFLEEVKKLKNEFE------DINELNL 468
            MAGSR EKF  +  R   L+++ VIK+   P+W+DVY AFPP+  P    +  R  P +    VP+I Y ED  RA F          F ++ IE++  D     FL   ++  +++       D+N  NL
Sbjct:    1 MAGSRLEKFGTVYTRVRDLMRSGVIKESQKPLWFDVYTAFPPKREPLYVKSSKRIWPQKRDDHVPEIFYKEDEIRAKF----------FKVYGIESRPFDLSKTYFLSTCQRFVDKYSELESRGDVNPENL 121
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BLAST of MRP-S23 domain-containing protein vs. Ensembl Medaka
Match: mrps23 (mitochondrial ribosomal protein S23 [Source:NCBI gene;Acc:101162150])

HSP 1 Score: 56.6102 bits (135), Expect = 9.358e-10
Identity = 32/74 (43.24%), Postives = 44/74 (59.46%), Query Frame = 1 
Query:  130 SRKEKFIPLIERATGLLKNKVIKDVDTPIWYDVYKAFPPEVSPNF----NRPIPNQN--VPKILYPEDVHRAFF 333
            SR E+F  +  R   L+++ VI+  D PIWYDVY+ FPP+  P +    +RP  N+   VP+I Y ED  RA F
Sbjct:   85 SRLERFGTVFSRVRDLMRSGVIQKADKPIWYDVYEVFPPKREPLYVKQQSRPAVNKQDPVPEIFYREDEVRAKF 158
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BLAST of MRP-S23 domain-containing protein vs. Planmine SMEST
Match: SMESG000056643.1 (SMESG000056643.1)

HSP 1 Score: 255.373 bits (651), Expect = 1.045e-88
Identity = 127/127 (100.00%), Postives = 127/127 (100.00%), Query Frame = 1 
Query:  121 MAGSRKEKFIPLIERATGLLKNKVIKDVDTPIWYDVYKAFPPEVSPNFNRPIPNQNVPKILYPEDVHRAFFIGHPLYRNAIFNLFHIENKDNYSYKFLEEVKKLKNEFEDINELNLNAIRNLSSNLS 501
            MAGSRKEKFIPLIERATGLLKNKVIKDVDTPIWYDVYKAFPPEVSPNFNRPIPNQNVPKILYPEDVHRAFFIGHPLYRNAIFNLFHIENKDNYSYKFLEEVKKLKNEFEDINELNLNAIRNLSSNLS
Sbjct:    1 MAGSRKEKFIPLIERATGLLKNKVIKDVDTPIWYDVYKAFPPEVSPNFNRPIPNQNVPKILYPEDVHRAFFIGHPLYRNAIFNLFHIENKDNYSYKFLEEVKKLKNEFEDINELNLNAIRNLSSNLS 127
The following BLAST results are available for this feature:
BLAST of MRP-S23 domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2
Match NameE-valueIdentityDescription
MRPS235.885e-833.90mitochondrial ribosomal protein S23 [Source:HGNC S... [more]
MRPS232.683e-733.90mitochondrial ribosomal protein S23 [Source:HGNC S... [more]
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BLAST of MRP-S23 domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2
Match NameE-valueIdentityDescription
mrps-231.405e-932.08Probable 28S ribosomal protein S23, mitochondrial ... [more]
mrps-231.405e-932.08Probable 28S ribosomal protein S23, mitochondrial ... [more]
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BLAST of MRP-S23 domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mRpS236.176e-1752.17gene:FBgn0260407 transcript:FBtr0080566[more]
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BLAST of MRP-S23 domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
mrps233.581e-1246.05mitochondrial ribosomal protein S23 [Source:ZFIN;A... [more]
mrps233.673e-1246.05mitochondrial ribosomal protein S23 [Source:ZFIN;A... [more]
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BLAST of MRP-S23 domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
casz15.329e-1444.74castor zinc finger 1 [Source:Xenbase;Acc:XB-GENE-9... [more]
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BLAST of MRP-S23 domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Mrps233.140e-1134.75mitochondrial ribosomal protein S23 [Source:MGI Sy... [more]
Mrps233.140e-1134.75mitochondrial ribosomal protein S23 [Source:MGI Sy... [more]
Mrps238.077e-1137.36mitochondrial ribosomal protein S23 [Source:MGI Sy... [more]
Mrps238.077e-1137.36mitochondrial ribosomal protein S23 [Source:MGI Sy... [more]
Mrps231.219e-734.31mitochondrial ribosomal protein S23 [Source:MGI Sy... [more]
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BLAST of MRP-S23 domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 4
Match NameE-valueIdentityDescription
sp|Q8VE22|RT23_MOUSE2.197e-1034.7528S ribosomal protein S23, mitochondrial OS=Mus mu... [more]
sp|P34748|RT23_CAEEL1.787e-832.08Probable 28S ribosomal protein S23, mitochondrial ... [more]
sp|Q9Y3D9|RT23_HUMAN1.288e-633.9028S ribosomal protein S23, mitochondrial OS=Homo s... [more]
sp|Q2NL27|RT23_BOVIN1.452e-634.7128S ribosomal protein S23, mitochondrial OS=Bos ta... [more]
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BLAST of MRP-S23 domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A430QUH69.576e-2340.71Small subunit ribosomal protein S23 OS=Schistosoma... [more]
A0A183LPI21.811e-2241.59MRP-S23 domain-containing protein OS=Schistosoma m... [more]
A0A183P5S73.898e-2240.71MRP-S23 domain-containing protein OS=Schistosoma m... [more]
A0A183QVY77.612e-2239.82MRP-S23 domain-containing protein OS=Schistosoma r... [more]
G4V6P83.448e-2138.94MRP-S23 domain-containing protein OS=Schistosoma m... [more]
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BLAST of MRP-S23 domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mrps233.653e-1133.59mitochondrial ribosomal protein S23 [Source:NCBI g... [more]
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BLAST of MRP-S23 domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MRP-S23 domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MRP-S23 domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MRP-S23 domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mrps239.358e-1043.24mitochondrial ribosomal protein S23 [Source:NCBI g... [more]
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BLAST of MRP-S23 domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000056643.11.045e-88100.00SMESG000056643.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30020040 ID=SMED30020040|Name=MRP-S23 domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=515bp
ATTTTTGAATTTCATTCTAAGTTATTTTTATATTTAAAATTACATTTTAA
CTATAAAATTACATTATTAATTTAGTATCTTATAATTATAAATATTTTTT
TGTTTACATAATAAAAATTAATGGCTGGAAGTCGCAAAGAAAAGTTTATT
CCTTTAATCGAACGAGCAACAGGTCTTTTGAAGAATAAAGTTATTAAAGA
TGTAGATACACCGATATGGTATGATGTATATAAAGCATTTCCTCCTGAAG
TTTCTCCTAATTTTAACCGCCCTATTCCAAATCAAAATGTACCTAAGATT
CTTTATCCTGAAGATGTACATAGAGCATTTTTTATTGGGCATCCGTTGTA
TCGTAACGCAATTTTCAATTTATTTCATATTGAAAATAAGGATAATTATT
CATATAAATTTTTAGAAGAAGTCAAGAAGCTCAAAAATGAATTTGAAGAT
ATCAATGAACTAAATTTAAACGCAATCCGCAATTTAAGCAGTAATTTAAG
TTAGGTAAAAAAGAC
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protein sequence of SMED30020040-orf-1

>SMED30020040-orf-1 ID=SMED30020040-orf-1|Name=SMED30020040-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=128bp
MAGSRKEKFIPLIERATGLLKNKVIKDVDTPIWYDVYKAFPPEVSPNFNR
PIPNQNVPKILYPEDVHRAFFIGHPLYRNAIFNLFHIENKDNYSYKFLEE
VKKLKNEFEDINELNLNAIRNLSSNLS*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: cellular component
TermDefinition
GO:0005739mitochondrion
GO:0005840ribosome
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR019520Ribosomal_S23
IPR023611Ribosomal_S23/S25_mit
Vocabulary: molecular function
TermDefinition
GO:0003735structural constituent of ribosome
Vocabulary: biological process
TermDefinition
GO:0006412translation
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 101..121
IPR023611Ribosomal protein S23/S25, mitochondrialPFAMPF10484MRP-S23coord: 2..113
e-value: 1.1E-26
score: 93.6
IPR019520Ribosomal protein S23, mitochondrialPANTHERPTHR15925MITOCHONDRIAL RIBOSOMAL PROTEIN S23coord: 1..113