SMED30020016

Overview
NameSMED30020016
Smed IDSMED30020016
Length (bp)2111
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30020016 (SMED30020016) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30020016 (SMED30020016) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30020016

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parenchymaSMED30020016 dd_Smed_v4_26699_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30020016 vs. Ensembl Zebrafish
Match: BX511224.1 (pep chromosome:GRCz11:2:18017000:18022765:1 gene:ENSDARG00000113243.1 transcript:ENSDART00000186877.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:BX511224.1)

HSP 1 Score: 90.1225 bits (222), Expect = 4.809e-28
Identity = 40/113 (35.40%), Postives = 69/113 (61.06%), Query Frame = -2 
Query: 1718 MMPFGIAEAPAIFQRLMNKVLEEINWKIVIVYLDDIFICSRDYESHIRDIEHVFIKIEKAGLKISSSKCKFLRTEVRFLEHIINETGLKTDTSKIDSIKHFQTPQVSRNFEDF 2056
            ++PFG++ APA+FQ L+N VL ++  + + VYLDDI I S   + HI+ +  V  ++ + GL + + KC F    V+FL HI++  G++ D  KI ++ ++ TP   +  + F
Sbjct:  645 VLPFGLSNAPAVFQALVNDVLRDMIDQFIYVYLDDILIFSHSLQEHIQHVRRVLQRLLENGLYVKAEKCVFHAQSVQFLGHIVSVEGMRMDPEKIQAVVNWPTPDSRKALQRF 757

HSP 2 Score: 55.0694 bits (131), Expect = 4.809e-28
Identity = 32/84 (38.10%), Postives = 48/84 (57.14%), Query Frame = -3 
Query: 1474 KILGLTNCYRKFIDNDTKYSKPLESLTSSQSRKLLGSEECQKAFV*LKEKLCMAPILICTDLN*SFILDNDASFDRIGVVLDEK 1725
            + LG  N YR+FI N ++ + PL SLTSS++     S   + AF  LK     APILI  D +  F+++ DAS   +G +L ++
Sbjct:  756 RFLGFANFYRRFIHNFSQLAAPLTSLTSSKT-PFRWSSAAEAAFSKLKGCFVSAPILIAPDPSRQFVVEVDASEVGVGAILSQR 838
[Close]
BLAST of SMED30020016 vs. Ensembl Zebrafish
Match: BX546500.1 (pep chromosome:GRCz11:23:12926092:12931693:-1 gene:ENSDARG00000086495.3 transcript:ENSDART00000122176.3 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:BX546500.1)

HSP 1 Score: 88.9669 bits (219), Expect = 3.021e-27
Identity = 39/113 (34.51%), Postives = 68/113 (60.18%), Query Frame = -2 
Query: 1718 MMPFGIAEAPAIFQRLMNKVLEEINWKIVIVYLDDIFICSRDYESHIRDIEHVFIKIEKAGLKISSSKCKFLRTEVRFLEHIINETGLKTDTSKIDSIKHFQTPQVSRNFEDF 2056
            ++PFG++ APA+FQ L+N VL ++  + + VYLDDI I S   + H++ +  V  ++ + GL + + KC F    V+FL HI++  G++ D  KI ++  + TP   +  + F
Sbjct:  619 VLPFGLSNAPAVFQALVNDVLRDMIDQFIYVYLDDILIFSHSLQEHVQHVRRVLQRLLENGLYVKAEKCVFHAQSVQFLGHIVSVEGMRMDPEKIQAVVDWPTPDSRKALQRF 731

HSP 2 Score: 53.9138 bits (128), Expect = 3.021e-27
Identity = 32/84 (38.10%), Postives = 47/84 (55.95%), Query Frame = -3 
Query: 1474 KILGLTNCYRKFIDNDTKYSKPLESLTSSQSRKLLGSEECQKAFV*LKEKLCMAPILICTDLN*SFILDNDASFDRIGVVLDEK 1725
            + LG  N YR+FI N ++ + PL SLTSS+      S   + AF  LK     APILI  D +  F+++ DAS   +G +L ++
Sbjct:  730 RFLGFANFYRRFIRNFSQLAAPLTSLTSSK-MPFRWSSAAEAAFSKLKGCFVSAPILIAPDPSRQFVVEVDASEVGVGAILSQR 812
[Close]
BLAST of SMED30020016 vs. Ensembl Zebrafish
Match: CR855320.1 (pep chromosome:GRCz11:1:7956030:7961696:1 gene:ENSDARG00000099359.2 transcript:ENSDART00000159655.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CR855320.1)

HSP 1 Score: 88.1965 bits (217), Expect = 6.077e-27
Identity = 40/113 (35.40%), Postives = 67/113 (59.29%), Query Frame = -2 
Query: 1718 MMPFGIAEAPAIFQRLMNKVLEEINWKIVIVYLDDIFICSRDYESHIRDIEHVFIKIEKAGLKISSSKCKFLRTEVRFLEHIINETGLKTDTSKIDSIKHFQTPQVSRNFEDF 2056
            ++PFG++ APA+FQ  +N VL ++  + + VYLDDI I S   + H++ I  V  ++ + GL + + KC F    V FL HI++  GL+ D  KI ++ ++ TP   +  + F
Sbjct:  647 VLPFGLSNAPAVFQAFVNDVLRDMIDQFIYVYLDDILIFSHSLQEHVQHIRRVLQRLLENGLYVKAEKCVFHAQSVPFLGHIVSVEGLRMDPEKIKAVVNWPTPDSRKALQRF 759

HSP 2 Score: 53.5286 bits (127), Expect = 6.077e-27
Identity = 31/84 (36.90%), Postives = 48/84 (57.14%), Query Frame = -3 
Query: 1474 KILGLTNCYRKFIDNDTKYSKPLESLTSSQSRKLLGSEECQKAFV*LKEKLCMAPILICTDLN*SFILDNDASFDRIGVVLDEK 1725
            + LG  N YR+FI N ++ + PL +LTSS++     S   + AF  LK     APILI  D +  F+++ DAS   +G +L ++
Sbjct:  758 RFLGFANFYRRFIRNFSQLAAPLTALTSSKT-PFRWSSAAEAAFSKLKGCFVSAPILITPDPSRQFVVEVDASEVGVGAILSQR 840
[Close]
BLAST of SMED30020016 vs. Ensembl Zebrafish
Match: FO704673.1 (pep chromosome:GRCz11:12:14545475:14551077:-1 gene:ENSDARG00000112601.1 transcript:ENSDART00000188717.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:FO704673.1)

HSP 1 Score: 87.0409 bits (214), Expect = 1.579e-26
Identity = 37/114 (32.46%), Postives = 70/114 (61.40%), Query Frame = -2 
Query: 1718 LMMPFGIAEAPAIFQRLMNKVLEEINWKIVIVYLDDIFICSRDYESHIRDIEHVFIKIEKAGLKISSSKCKFLRTEVRFLEHIINETGLKTDTSKIDSIKHFQTPQVSRNFEDF 2059
            L+MP+G+A +PA+FQ  +N+V  ++  K VI Y+DDI I S + E HI+D+  V  ++++  L     KC+F  ++  FL +II+  G++ + +K+ ++  +  P+  +  + F
Sbjct:  623 LVMPYGLANSPAVFQSFINEVFRDLLNKCVIAYIDDILIYSPNLEQHIKDVRTVLTRLQENQLYAKLEKCEFHMSKTSFLGYIISHHGVEMNNTKVQAVTGWPLPKTVKELQRF 736

HSP 2 Score: 53.1434 bits (126), Expect = 1.579e-26
Identity = 33/87 (37.93%), Postives = 45/87 (51.72%), Query Frame = -3 
Query: 1474 ETSKILGLTNCYRKFIDNDTKYSKPLESLTSSQSRKLLGSEECQKAFV*LKEKLCMAPILICTDLN*SFILDNDASFDRIGVVLDEK 1734
            E  + LG  N YR+FI N +  S PL SL   +  KL  + E  K+F  LK     APIL   +    F+++ DAS   IG VL ++
Sbjct:  732 ELQRFLGFANFYRRFIRNYSLISAPLTSLLKGKPSKLKWNPETVKSFEKLKTSFTTAPILKHPNPELPFVVEVDASDYGIGAVLSQR 818
[Close]
BLAST of SMED30020016 vs. Ensembl Zebrafish
Match: BX511082.1 (pep chromosome:GRCz11:9:14291932:14297132:1 gene:ENSDARG00000113678.1 transcript:ENSDART00000183119.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:BX511082.1)

HSP 1 Score: 87.0409 bits (214), Expect = 3.811e-26
Identity = 38/113 (33.63%), Postives = 67/113 (59.29%), Query Frame = -2 
Query: 1718 MMPFGIAEAPAIFQRLMNKVLEEINWKIVIVYLDDIFICSRDYESHIRDIEHVFIKIEKAGLKISSSKCKFLRTEVRFLEHIINETGLKTDTSKIDSIKHFQTPQVSRNFEDF 2056
            ++PFG++ APA+FQ L+N VL ++  + + VYLDDI I S   + H++ +  V  ++ + GL + + KC F    V FL HI++  G++ D  K+ ++  + TP   +  + F
Sbjct:  645 VLPFGLSNAPAVFQALVNDVLRDMLDQFIYVYLDDILIFSHSLQEHVQHVRRVLQRLLENGLYVKAEKCVFHAQSVPFLGHIVSVEGMRMDPEKVQAVVDWPTPDSRKALQRF 757

HSP 2 Score: 51.9878 bits (123), Expect = 3.811e-26
Identity = 31/84 (36.90%), Postives = 47/84 (55.95%), Query Frame = -3 
Query: 1474 KILGLTNCYRKFIDNDTKYSKPLESLTSSQSRKLLGSEECQKAFV*LKEKLCMAPILICTDLN*SFILDNDASFDRIGVVLDEK 1725
            + LG  N YR+FI N ++ + PL +LTS ++     S   Q AF  LK     APILI  D +  F+++ DAS   +G +L ++
Sbjct:  756 RFLGFANFYRRFIRNFSQLAAPLTALTSLKT-PFRWSNAAQVAFDRLKSCFVSAPILIAPDPSRQFVVEVDASEVGVGAILSQR 838
[Close]
BLAST of SMED30020016 vs. Ensembl Xenopus
Match: ENSXETT00000006041.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:6:37317867:37322724:1 gene:ENSXETG00000003070.1 transcript:ENSXETT00000006041.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 91.6633 bits (226), Expect = 1.698e-25
Identity = 41/112 (36.61%), Postives = 68/112 (60.71%), Query Frame = -2 
Query: 1718 MPFGIAEAPAIFQRLMNKVLEEINWKIVIVYLDDIFICSRDYESHIRDIEHVFIKIEKAGLKISSSKCKFLRTEVRFLEHIINETGLKTDTSKIDSIKHFQTPQVSRNFEDF 2053
            MP G+  APA FQRLM + + +++   V+VYLDD+ +  R  E H + +  VF ++EK GLK+S  KC+F +  V ++ H+++  G+ TD SKI+++  +  P+       F
Sbjct:  448 MPQGLCGAPATFQRLMERTVGDMHLLEVLVYLDDLIVFGRTLEEHEQRLLKVFDRLEKEGLKLSPEKCQFCQPSVNYVGHVVSAEGIATDPSKIEAVSSWPKPRTITELRSF 559

HSP 2 Score: 45.4394 bits (106), Expect = 1.698e-25
Identity = 45/176 (25.57%), Postives = 78/176 (44.32%), Query Frame = -3 
Query: 1303 ETSKILGLTNCYRKFIDNDTKYSKPLESLTSSQ-----------SRKLLG------------SEECQKAFV*LKEKLCMAPILICTDLN*SFILDNDASFDRIGVVLDEK*KG*LHMGQKHSININLNIALQEKFF*PH-------QFTVYFK--HYLYGKRFKLRSNQKALTFMI 1734
            E    LG    YR+F+   +K ++PL  L  S            +++L G            +EEC KAF  LK  L  AP+L   D    + L  DAS + +G +L ++    L    +    I+ +++  E+ +  H       ++ V  K   YLYG  F+++++   LT+++
Sbjct:  555 ELRSFLGFCGYYRRFVKGFSKVAQPLNQLLQSNTEVEGSDRDILAKRLRGQGWTRESIEDFWTEECDKAFDQLKYCLTHAPVLAYADATKPYTLHIDASREGLGGILYQEYNKEL----RPVAFISRSLSPSERNYPAHKLEFLALKWAVVDKLHEYLYGAEFEVQTDNNPLTYIL 726
[Close]
BLAST of SMED30020016 vs. Ensembl Xenopus
Match: anxa6 (annexin A6 [Source:Xenbase;Acc:XB-GENE-989741])

HSP 1 Score: 89.3521 bits (220), Expect = 9.152e-18
Identity = 40/113 (35.40%), Postives = 67/113 (59.29%), Query Frame = -2 
Query: 1718 MMPFGIAEAPAIFQRLMNKVLEEINWKIVIVYLDDIFICSRDYESHIRDIEHVFIKIEKAGLKISSSKCKFLRTEVRFLEHIINETGLKTDTSKIDSIKHFQTPQVSRNFEDF 2056
            +MPFG+  APA FQRL+N++LE +       YLDDI + S+ +E H++ ++ VF +I+ AGL +   KC     EV++L H +    L+ D +K+++I  +  P+  +    F
Sbjct:  458 VMPFGMRNAPATFQRLVNRLLEGMQ-DFAQAYLDDIAVFSQTWEEHLQHLQRVFAQIQDAGLTLKPEKCHLAMAEVQYLGHRVGGGQLRPDPAKVEAICQWPIPKTQKQVLAF 569

HSP 2 Score: 64.6994 bits (156), Expect = 3.821e-10
Identity = 38/92 (41.30%), Postives = 52/92 (56.52%), Query Frame = -3 
Query: 1477 PETSK----ILGLTNCYRKFIDNDTKYSKPLESLTSSQ-SRKLLGSEECQKAFV*LKEKLCMAPILICTDLN*SFILDNDASFDRIGVVLDE 1737
            P+T K     LG +  YRKFI N +  +KPL  LTS Q SR ++ + EC+ A   LK+ L  +P+L   D +  FIL  DAS   +G VL +
Sbjct:  560 PKTQKQVLAFLGTSGYYRKFIPNYSTVAKPLTDLTSRQRSRTIVWTPECESAMNALKQALASSPVLAAPDFSRRFILQTDASNFGLGAVLSQ 651
[Close]
BLAST of SMED30020016 vs. Ensembl Xenopus
Match: denr (density-regulated protein [Source:Xenbase;Acc:XB-GENE-1002798])

HSP 1 Score: 87.8113 bits (216), Expect = 3.560e-17
Identity = 39/114 (34.21%), Postives = 67/114 (58.77%), Query Frame = -2 
Query: 1718 LMMPFGIAEAPAIFQRLMNKVLEEINWKIVIVYLDDIFICSRDYESHIRDIEHVFIKIEKAGLKISSSKCKFLRTEVRFLEHIINETGLKTDTSKIDSIKHFQTPQVSRNFEDF 2059
            L+MPFG+  APA+FQ  +N +  ++  K V+VYLDDI I S+D E+H   ++    ++ +  L     KC F   ++ FL +II+  G + D +K+ +I+ +  PQ ++  + F
Sbjct:  640 LVMPFGLCNAPAVFQEFVNDIFRDLLGKSVVVYLDDILIFSQDLETHRSQVKEALSRLRENSLFAKLDKCTFEVPKISFLGYIISSRGFEMDPAKVSAIQKWPLPQSTKAIQRF 753
[Close]
BLAST of SMED30020016 vs. Ensembl Xenopus
Match: castor1 (cytosolic arginine sensor for mTORC1 subunit 1 [Source:Xenbase;Acc:XB-GENE-960689])

HSP 1 Score: 87.8113 bits (216), Expect = 4.064e-17
Identity = 39/114 (34.21%), Postives = 67/114 (58.77%), Query Frame = -2 
Query: 1718 LMMPFGIAEAPAIFQRLMNKVLEEINWKIVIVYLDDIFICSRDYESHIRDIEHVFIKIEKAGLKISSSKCKFLRTEVRFLEHIINETGLKTDTSKIDSIKHFQTPQVSRNFEDF 2059
            L+MPFG+  APA+FQ  +N +  ++  K V+VYLDDI I S+D E+H   ++    ++ +  L     KC F   ++ FL +II+  G + D +K+ +I+ +  PQ ++  + F
Sbjct:  640 LVMPFGLCNAPAVFQEFVNDIFRDLLGKSVVVYLDDILIFSQDLETHRSQVKEALSRLRENSLFAKLDKCTFEVPKISFLGYIISSRGFEMDPAKVSAIQKWPLPQSTKAIQRF 753
[Close]
BLAST of SMED30020016 vs. Ensembl Xenopus
Match: ENSXETT00000020886.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:3:49613601:49618752:-1 gene:ENSXETG00000011904.1 transcript:ENSXETT00000020886.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 87.8113 bits (216), Expect = 4.064e-17
Identity = 39/114 (34.21%), Postives = 67/114 (58.77%), Query Frame = -2 
Query: 1718 LMMPFGIAEAPAIFQRLMNKVLEEINWKIVIVYLDDIFICSRDYESHIRDIEHVFIKIEKAGLKISSSKCKFLRTEVRFLEHIINETGLKTDTSKIDSIKHFQTPQVSRNFEDF 2059
            L+MPFG+  APA+FQ  +N +  ++  K V+VYLDDI I S+D E+H   ++    ++ +  L     KC F   ++ FL +II+  G + D +K+ +I+ +  PQ ++  + F
Sbjct:  640 LVMPFGLCNAPAVFQEFVNDIFRDLLGKSVVVYLDDILIFSQDLETHRSQVKEALSRLRENSLFAKLDKCTFEVPKISFLGYIISSRGFEMDPAKVSAIQKWPLPQSTKAIQRF 753
[Close]
BLAST of SMED30020016 vs. UniProt/SwissProt
Match: sp|P20825|POL2_DROME (Retrovirus-related Pol polyprotein from transposon 297 OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 92.0485 bits (227), Expect = 1.923e-27
Identity = 46/114 (40.35%), Postives = 63/114 (55.26%), Query Frame = -2 
Query: 1718 LMMPFGIAEAPAIFQRLMNKVLEEINWKIVIVYLDDIFICSRDYESHIRDIEHVFIKIEKAGLKISSSKCKFLRTEVRFLEHIINETGLKTDTSKIDSIKHFQTPQVSRNFEDF 2059
            L MPFG+  APA FQR MN +L  +  K  +VYLDDI I S     H+  I+ VF K+  A LK+   KC+FL+ E  FL HI+   G+K +  K+ +I  +  P   +    F
Sbjct:  331 LRMPFGLRNAPATFQRCMNNILRPLLNKHCLVYLDDIIIFSTSLTEHLNSIQLVFTKLADANLKLQLDKCEFLKKEANFLGHIVTPDGIKPNPIKVKAIVSYPIPTKDKEIRAF 444

HSP 2 Score: 53.9138 bits (128), Expect = 1.923e-27
Identity = 43/146 (29.45%), Postives = 69/146 (47.26%), Query Frame = -3 
Query: 1306 ETSKILGLTNCYRKFIDNDTKYSKPLESLTSSQSRKLLGSEECQKAFV*LKEKLCMAPILICTDLN*SFILDNDASFDRIGVVLDEK*KG*LHMGQ---KHSININLNIALQEKFF*PHQFTVYFKHYLYGKRFKLRSNQKALTFM 1734
            E    LGLT  YRKFI N    +KP+ S    +++      E  +AF  LK  +   PIL   D    F+L  DAS   +G VL +       + +    H +N +   A++++       T  F+HYL G++F + S+ + L ++
Sbjct:  440 EIRAFLGLTGYYRKFIPNYADIAKPMTSCLKKRTKIDTQKLEYIEAFEKLKALIIRDPILQLPDFEKKFVLTTDASNLALGAVLSQNGHPISFISRTLNDHELNYS---AIEKELLAIVWATKTFRHYLLGRQFLIASDHQPLRWL 582
[Close]
BLAST of SMED30020016 vs. UniProt/SwissProt
Match: sp|Q8I7P9|POL5_DROME (Retrovirus-related Pol polyprotein from transposon opus OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 1.906e-25
Identity = 42/114 (36.84%), Postives = 68/114 (59.65%), Query Frame = -2 
Query: 1718 LMMPFGIAEAPAIFQRLMNKVLEEINWKIVIVYLDDIFICSRDYESHIRDIEHVFIKIEKAGLKISSSKCKFLRTEVRFLEHIINETGLKTDTSKIDSIKHFQTPQVSRNFEDF 2059
            L +PFG+  APAIFQR+++ +L E   K+  VY+DDI + S DY++H +++  V   + KA L+++  K  FL T+V FL +I+   G+K D  K+ +I     P   +  + F
Sbjct:  248 LRLPFGLKNAPAIFQRMIDDILREHIGKVCYVYIDDIIVFSEDYDTHWKNLRLVLASLSKANLQVNLEKSHFLDTQVEFLGYIVTADGIKADPKKVRAISEMPPPTSVKELKRF 361

HSP 2 Score: 48.1358 bits (113), Expect = 1.906e-25
Identity = 38/100 (38.00%), Postives = 51/100 (51.00%), Query Frame = -3 
Query: 1465 ETSKILGLTNCYRKFIDNDTKYSKPLESLT--------SSQSRKLLGS--EECQKAFV*LKEKLCMAPILICTDLN*SFILDNDASFDRIGVVLDEK*KG 1734
            E  + LG+T+ YRKFI +  K +KPL +LT        SSQS K+  +  E   ++F  LK  LC + IL        F L  DAS   IG VL +  +G
Sbjct:  357 ELKRFLGMTSYYRKFIQDYAKVAKPLTNLTRGLYANIKSSQSSKVPITLDETALQSFNDLKSILCSSEILAFPCFTKPFHLTTDASNWAIGAVLSQDDQG 456
[Close]
BLAST of SMED30020016 vs. UniProt/SwissProt
Match: sp|P10394|POL4_DROME (Retrovirus-related Pol polyprotein from transposon 412 OS=Drosophila melanogaster OX=7227 GN=POL PE=4 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 1.020e-19
Identity = 32/103 (31.07%), Postives = 51/103 (49.51%), Query Frame = -2 
Query: 1745 MPFGIAEAPAIFQRLMNKVLEEINWKIVIVYLDDIFICSRDYESHIRDIEHVFIKIEKAGLKISSSKCKFLRTEVRFLEHIINETGLKTDTSKIDSIKHFQTP 2053
            +PFG+  AP  FQR+M      I      +Y+DD+ +     +  ++++  VF K  +  LK+   KC F   EV FL H   + G+  D  K D I+++  P
Sbjct:  442 LPFGLKIAPNSFQRMMTIAFSGIEPSQAFLYMDDLIVIGCSEKHMLKNLTEVFGKCREYNLKLHPEKCSFFMHEVTFLGHKCTDKGILPDDKKYDVIQNYPVP 544

HSP 2 Score: 55.4546 bits (132), Expect = 1.020e-19
Identity = 37/144 (25.69%), Postives = 70/144 (48.61%), Query Frame = -3 
Query: 1306 KILGLTNCYRKFIDNDTKYSKPLESLTSSQSRKLLGSEECQKAFV*LKEKLCMAPILICTDLN*SFILDNDASFDRIGVVLDEK*KG----*LHMGQKHSININLNIALQEKFF*PHQFTVYFKHYLYGKRFKLRSNQKALTFM 1725
            + +   N YR+FI N   YS+ +  L   ++     ++ECQKAF+ LK +L    +L   D +  F +  DAS    G VL +   G      +  +  +   +     +++    H   ++F+ Y+YGK F ++++ + LT++
Sbjct:  552 RFVAFCNYYRRFIKNFADYSRHITRL-CKKNVPFEWTDECQKAFIHLKSQLINPTLLQYPDFSKEFCITTDASKQACGAVLTQNHNGHQLPVAYASRAFTKGESNKSTTEQELAAIHWAIIHFRPYIYGKHFTVKTDHRPLTYL 694
[Close]
BLAST of SMED30020016 vs. UniProt/SwissProt
Match: sp|P04323|POL3_DROME (Retrovirus-related Pol polyprotein from transposon 17.6 OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 1.097e-18
Identity = 45/114 (39.47%), Postives = 68/114 (59.65%), Query Frame = -2 
Query: 1718 LMMPFGIAEAPAIFQRLMNKVLEEINWKIVIVYLDDIFICSRDYESHIRDIEHVFIKIEKAGLKISSSKCKFLRTEVRFLEHIINETGLKTDTSKIDSIKHFQTPQVSRNFEDF 2059
            L MPFG+  APA FQR MN +L  +  K  +VYLDDI + S   + H++ +  VF K+ KA LK+   KC+FL+ E  FL H++   G+K +  KI++I+ +  P   +  + F
Sbjct:  332 LRMPFGLKNAPATFQRCMNDILRPLLNKHCLVYLDDIIVFSTSLDEHLQSLGLVFEKLAKANLKLQLDKCEFLKQETTFLGHVLTPDGIKPNPEKIEAIQKYPIPTKPKEIKAF 445

HSP 2 Score: 54.6842 bits (130), Expect = 2.734e-6
Identity = 46/158 (29.11%), Postives = 73/158 (46.20%), Query Frame = -3 
Query: 1306 SIFKHP---KCPETSKILGLTNCYRKFIDNDTKYSKPLESLTSSQSRKLLGSEECQKAFV*LKEKLCMAPILICTDLN*SFILDNDASFDRIGVVLDEK*KG*LHMGQ---KHSININLNIALQEKFF*PHQFTVYFKHYLYGKRFKLRSNQKALTFM 1761
            +I K+P   K  E    LGLT  YRKFI N    +KP+        +    + E   AF  LK  +   PIL   D    F L  DAS   +G VL +      ++ +   +H IN +    ++++       T  F+HYL G+ F++ S+ + L+++
Sbjct:  429 AIQKYPIPTKPKEIKAFLGLTGYYRKFIPNFADIAKPMTKCLKKNMKIDTTNPEYDSAFKKLKYLISEDPILKVPDFTKKFTLTTDASDVALGAVLSQDGHPLSYISRTLNEHEINYS---TIEKELLAIVWATKTFRHYLLGRHFEISSDHQPLSWL 583
[Close]
BLAST of SMED30020016 vs. UniProt/SwissProt
Match: sp|P0CT39|TF26_SCHPO (Transposon Tf2-6 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=Tf2-6 PE=3 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 1.670e-14
Identity = 38/114 (33.33%), Postives = 67/114 (58.77%), Query Frame = -2 
Query: 1718 LMMPFGIAEAPAIFQRLMNKVLEEINWKIVIVYLDDIFICSRDYESHIRDIEHVFIKIEKAGLKISSSKCKFLRTEVRFLEHIINETGLKTDTSKIDSIKHFQTPQVSRNFEDF 2059
            L+MP+GI+ APA FQ  +N +L E     V+ Y+DDI I S+    H++ ++ V  K++ A L I+ +KC+F +++V+F+ + I+E G       ID +  ++ P+  +    F
Sbjct:  532 LVMPYGISTAPAHFQYFINTILGEAKESHVVCYMDDILIHSKSESEHVKHVKDVLQKLKNANLIINQAKCEFHQSQVKFIGYHISEKGFTPCQENIDKVLQWKQPKNRKELRQF 645
[Close]
BLAST of SMED30020016 vs. TrEMBL
Match: A0A087SUZ9 (Retrovirus-related Pol polyprotein from transposon 412 (Fragment) OS=Stegodyphus mimosarum OX=407821 GN=X975_16630 PE=4 SV=1)

HSP 1 Score: 105.145 bits (261), Expect = 3.079e-35
Identity = 43/113 (38.05%), Postives = 74/113 (65.49%), Query Frame = -2 
Query: 1718 MMPFGIAEAPAIFQRLMNKVLEEINWKIVIVYLDDIFICSRDYESHIRDIEHVFIKIEKAGLKISSSKCKFLRTEVRFLEHIINETGLKTDTSKIDSIKHFQTPQVSRNFEDF 2056
            +MPFG+  APA F+RLM  VL+ ++++  ++YLDDI I  + +E H+ ++  V  K+++A LK+S +KCK  R EV +L H+I+  G++TD  K+ ++K ++ P+       F
Sbjct:  791 VMPFGLCNAPATFERLMETVLKGLSYEACLIYLDDIIIVGKSFEEHLENLRKVLQKLKEANLKLSPAKCKLFRQEVTYLGHVISAEGVRTDPEKVSAVKDWRRPENVHQLRSF 903

HSP 2 Score: 75.0998 bits (183), Expect = 3.079e-35
Identity = 53/148 (35.81%), Postives = 84/148 (56.76%), Query Frame = -3 
Query: 1288 LGLTNCYRKFIDNDTKYSKPLESLTSSQSRKLLGSEECQKAFV*LKEKLCMAPILICTDLN*SFILDNDASFDRIGVVLDEK*KG*LHMGQKHSININ---LNIALQEKFF*PHQFTV-YFKHYLYGKRFKLRSNQKALTFMILNKEN 1719
            LGL   YR+F+ + +  ++PL  LT S+ +K + ++EC+ AF  LKE L  APIL    L+  FILD DAS + +G VL ++ +G   +    S  ++    N  +  K        V +F HYLYG++F LR++  +L ++ LN +N
Sbjct:  904 LGLCTYYRRFVKDFSSIARPLHKLTESK-QKFVWTKECENAFKNLKEALTSAPILTYPQLDRPFILDTDASNESVGAVLSQEIEGQERVVAYWSKCLSKPERNYCVTRKELLAIVKAVEHFHHYLYGRKFLLRTDHASLAWL-LNFKN 1049
[Close]
BLAST of SMED30020016 vs. TrEMBL
Match: A0A4Y2EP60 (Transposon Ty3-G Gag-Pol polyprotein OS=Araneus ventricosus OX=182803 GN=TY3B-G_616 PE=4 SV=1)

HSP 1 Score: 106.301 bits (264), Expect = 4.791e-35
Identity = 46/112 (41.07%), Postives = 72/112 (64.29%), Query Frame = -2 
Query: 1718 MPFGIAEAPAIFQRLMNKVLEEINWKIVIVYLDDIFICSRDYESHIRDIEHVFIKIEKAGLKISSSKCKFLRTEVRFLEHIINETGLKTDTSKIDSIKHFQTPQVSRNFEDF 2053
            MPFG+   PA F+RLM  VL  ++ +  +VYLDDI I  R +E H+ ++  VF +++KA LK+S  KC+F +TEV +L H+I+  G+KTD  KI ++  +  P++  +   F
Sbjct:    1 MPFGLCNTPATFERLMEAVLRGLSSEACLVYLDDIIIVGRTFEEHLNNLRKVFQRLQKANLKLSPKKCRFFQTEVTYLGHVISAEGVKTDPGKIKAVVDWPRPEMVHDLRSF 112

HSP 2 Score: 73.1738 bits (178), Expect = 4.791e-35
Identity = 46/154 (29.87%), Postives = 82/154 (53.25%), Query Frame = -3 
Query: 1285 ILGLTNCYRKFIDNDTKYSKPLESLTSSQSRKLLGSEECQKAFV*LKEKLCMAPILICTDLN*SFILDNDASFDRIGVVLDEK*KG*LHMGQKHSININLNIALQEKFF*PHQFTV--------YFKHYLYGKRFKLRSNQKALTFMILNKENQ 1722
             LG+   YR+F+ N +  ++PL  LT ++S     +EECQK+F+ L + L  +PIL C   +  FILD DA+ + IG VL +     +   ++     + ++   E+ +   +  +        +F HYLYG++F LR++  +L + +  KE +
Sbjct:  112 FLGMCTYYRRFVKNFSTIARPLHKLTEAKS-NFNWTEECQKSFISLNQALTSSPILTCPRTDKDFILDTDANNEGIGAVLSQN----IENEERVIAYFSKSLGKPERNYCVTRKELLAIVKSLEHFHHYLYGQKFLLRTDHASLRWPLNFKEPE 260
[Close]
BLAST of SMED30020016 vs. TrEMBL
Match: A0A087T293 (Retrovirus-related Pol polyprotein from transposon 412 (Fragment) OS=Stegodyphus mimosarum OX=407821 GN=X975_23274 PE=4 SV=1)

HSP 1 Score: 107.842 bits (268), Expect = 1.286e-34
Identity = 48/104 (46.15%), Postives = 72/104 (69.23%), Query Frame = -2 
Query: 1745 MMPFGIAEAPAIFQRLMNKVLEEINWKIVIVYLDDIFICSRDYESHIRDIEHVFIKIEKAGLKISSSKCKFLRTEVRFLEHIINETGLKTDTSKIDSIKHFQTP 2056
            +MPFG+  APA F+RLM  VL  ++++  +VYLDDI I  R +E H+ +I  VF K++ A LK+S SKCK  R+EV +L HII+  G++TD  K+ ++K ++ P
Sbjct:  821 VMPFGLCNAPATFERLMETVLRGLSYESCLVYLDDIIIVGRTFEEHLENIRKVFQKLKDANLKLSPSKCKLFRSEVTYLGHIISAEGVRTDPEKVLAVKDWERP 924

HSP 2 Score: 70.0922 bits (170), Expect = 1.286e-34
Identity = 49/148 (33.11%), Postives = 79/148 (53.38%), Query Frame = -3 
Query: 1288 LGLTNCYRKFIDNDTKYSKPLESLTSSQSRKLLGSEECQKAFV*LKEKLCMAPILICTDLN*SFILDNDASFDRIGVVLDEK*KG---*LHMGQKHSININLNIALQEK-FF*PHQFTVYFKHYLYGKRFKLRSNQKALTFMILNKEN 1719
            LGL   YR+F+   +  ++PL  LT ++ +K + +EEC+K+F  LK  L   P+L    ++  FILD DAS + +G VL +   G    +    K       N  +  K      +   +F HYLYG++F LR++  +LT++ LN +N
Sbjct:  934 LGLCTYYRRFVKGFSNIARPLHRLTENK-QKFVWTEECEKSFNELKTALTSTPVLAYPQIDKQFILDTDASNESVGAVLSQMIDGEERVIAYWSKCLSKPERNYCVTRKELLAIVKAIEHFHHYLYGRKFLLRTDHASLTWL-LNFKN 1079
[Close]
BLAST of SMED30020016 vs. TrEMBL
Match: A0A4Y2EIJ5 (Retrovirus-related Pol polyprotein from transposon 297 OS=Araneus ventricosus OX=182803 GN=pol_397 PE=4 SV=1)

HSP 1 Score: 106.301 bits (264), Expect = 1.798e-34
Identity = 48/113 (42.48%), Postives = 69/113 (61.06%), Query Frame = -2 
Query: 1718 MMPFGIAEAPAIFQRLMNKVLEEINWKIVIVYLDDIFICSRDYESHIRDIEHVFIKIEKAGLKISSSKCKFLRTEVRFLEHIINETGLKTDTSKIDSIKHFQTPQVSRNFEDF 2056
            +MPFG+  APA F+RLM  VL  +  +  +VYLDDI I  R ++ H+ +I  VF +++KA LK+S  KC F R EV +L HII+  G+KTD  K  ++  +  PQ   +   F
Sbjct:   99 VMPFGLCNAPATFERLMETVLRGLTSEACLVYLDDIIIVGRTFQEHLNNIRKVFQRLQKANLKLSPKKCSFFRKEVSYLGHIISADGVKTDPGKTKAVVDWPRPQTVHDLRSF 211

HSP 2 Score: 71.2478 bits (173), Expect = 1.798e-34
Identity = 46/154 (29.87%), Postives = 81/154 (52.60%), Query Frame = -3 
Query: 1291 ILGLTNCYRKFIDNDTKYSKPLESLTSSQSRKLLGSEECQKAFV*LKEKLCMAPILICTDLN*SFILDNDASFDRIGVVLDEK*KG*LHMGQKHSININLNIAL----------QEKFF*PHQFTVYFKHYLYGKRFKLRSNQKALTFMILNKE 1722
             LGL   YR+F+ N +  ++PL  LT +++     +EEC K+F  LK+ L  +P+L     +  FILD DAS + IG VL +K      +G +  +    + +L          +++     +   +F HYLYG++F LR++  +L +++  +E
Sbjct:  211 FLGLCTYYRRFVRNFSAIARPLHKLTEART-NFNWTEECDKSFNSLKQALITSPVLTYPRTDKEFILDTDASNEGIGAVLSQK------IGNEECVIAYFSKSLGKPERNYCVTRKELLAIVKSIEHFHHYLYGRKFLLRTDHASLRWLLNFRE 357
[Close]
BLAST of SMED30020016 vs. TrEMBL
Match: A0A4Y2DW63 (Retrovirus-related Pol polyprotein from transposon 297 (Fragment) OS=Araneus ventricosus OX=182803 GN=pol_77 PE=4 SV=1)

HSP 1 Score: 103.99 bits (258), Expect = 2.196e-34
Identity = 47/113 (41.59%), Postives = 69/113 (61.06%), Query Frame = -2 
Query: 1718 MMPFGIAEAPAIFQRLMNKVLEEINWKIVIVYLDDIFICSRDYESHIRDIEHVFIKIEKAGLKISSSKCKFLRTEVRFLEHIINETGLKTDTSKIDSIKHFQTPQVSRNFEDF 2056
            +MPFG+  APA F+RLM  VL  +  +  +VYLDDI I  R ++ H+ +I  VF +++KA LK+S  KC+F R EV +L HII+  G+KTD  K  ++  +  P    +   F
Sbjct:  141 VMPFGLCNAPATFERLMETVLRGLTSEACLVYLDDIIIVGRTFQEHLNNIRKVFQRLQKANLKLSPKKCRFFRKEVSYLGHIISADGVKTDPEKTKAVVDWPRPDTVHDVRSF 253

HSP 2 Score: 73.1738 bits (178), Expect = 2.196e-34
Identity = 47/156 (30.13%), Postives = 83/156 (53.21%), Query Frame = -3 
Query: 1285 ILGLTNCYRKFIDNDTKYSKPLESLTSSQSRKLLGSEECQKAFV*LKEKLCMAPILICTDLN*SFILDNDASFDRIGVVLDEK*KG*LHMGQKHSININLNIAL----------QEKFF*PHQFTVYFKHYLYGKRFKLRSNQKALTFMILNKENQ 1722
             LGL   YR+F+ N +  ++PL  LT ++S     +EEC+K+F  LK+ L  +P+L     +  FILD DAS + IG VL +K      +G +  +    + +L          +++     +   +F HYLYG++F LR++  +L +++  +E +
Sbjct:  253 FLGLCTYYRRFVRNFSAIARPLHKLTEARS-NFNWTEECEKSFNSLKQALITSPVLTYPRTDKEFILDTDASNEGIGAVLSQK------IGNEECVIAYFSKSLGKPERNHCVTRKELLAIVKSIEHFHHYLYGRKFLLRTDHASLRWLLNFREPE 401
[Close]
BLAST of SMED30020016 vs. Ensembl Cavefish
Match: ENSAMXT00000038088.1 (pep primary_assembly:Astyanax_mexicanus-2.0:23:13869500:13874588:-1 gene:ENSAMXG00000037864.1 transcript:ENSAMXT00000038088.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 104.375 bits (259), Expect = 2.606e-34
Identity = 46/112 (41.07%), Postives = 68/112 (60.71%), Query Frame = -2 
Query: 1718 MPFGIAEAPAIFQRLMNKVLEEINWKIVIVYLDDIFICSRDYESHIRDIEHVFIKIEKAGLKISSSKCKFLRTEVRFLEHIINETGLKTDTSKIDSIKHFQTPQVSRNFEDF 2053
            MPFG+  APA FQRLM   L     + + VYLDDI I S D+  H++ +E VF ++ + GLK+ S KCK  R +V+FL H++N+ G+  D  K+ +++ + TP   R    F
Sbjct:  459 MPFGLCNAPATFQRLMQHCLRGQVAEFLFVYLDDIIIHSADFSLHVQHLEQVFERLSRYGLKLRSDKCKLFRRQVKFLGHVVNKQGVHPDPEKVAAVQEWATPTTIREVRAF 570

HSP 2 Score: 62.003 bits (149), Expect = 2.606e-34
Identity = 51/168 (30.36%), Postives = 71/168 (42.26%), Query Frame = -3 
Query: 1306 ETSKILGLTNCYRKFIDNDTKYSKPLESL-------TSSQSRKLLGSEECQKAFV*LKEKLCMAPILICTDLN*SFILDNDASFDRIGVVLDEK*KG----------*LHMGQKHSIN--------INLNIALQEKFF*PHQFTVYFKHYLYGKRFKLRSNQKALTFM 1734
            E    LGL   YR+FID   K ++PL SL         S  R +  + ECQ AF  LK  L  APIL   D +  FI+  DAS   +G VL +   G           LH  +K+  N        + L  A+ EK          F+ YL G +F + ++   +  +
Sbjct:  566 EVRAFLGLAGYYRRFIDGFAKLARPLNSLLVGVPVNRKSSHRGINWTPECQNAFECLKSCLLQAPILAYADFSAPFIVYTDASSQGLGAVLSQMQDGRERVIAYASRSLHPSEKNDANYSSFKLELLALKWAVTEK----------FRDYLMGSKFMVMTDNNPVAHL 723
[Close]
BLAST of SMED30020016 vs. Ensembl Cavefish
Match: ENSAMXT00000037150.1 (pep primary_assembly:Astyanax_mexicanus-2.0:10:22265216:22268869:1 gene:ENSAMXG00000033912.1 transcript:ENSAMXT00000037150.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 101.293 bits (251), Expect = 5.604e-32
Identity = 40/103 (38.83%), Postives = 69/103 (66.99%), Query Frame = -2 
Query: 1745 MPFGIAEAPAIFQRLMNKVLEEINWKIVIVYLDDIFICSRDYESHIRDIEHVFIKIEKAGLKISSSKCKFLRTEVRFLEHIINETGLKTDTSKIDSIKHFQTP 2053
            MPFG+  APA FQRLM ++  +  ++ V++YLDD+ + S   E H+  +  VF +++K GLK+  SKC F + +V +L H+++  G+ TD +KID+++ ++ P
Sbjct:  445 MPFGLCNAPATFQRLMERMFGDCRYQSVLLYLDDVIVFSSSVEQHLERLAEVFSRLQKQGLKVKLSKCHFFQPQVNYLGHVVSREGVATDPAKIDAVRGWRRP 547

HSP 2 Score: 57.3806 bits (137), Expect = 5.604e-32
Identity = 35/106 (33.02%), Postives = 52/106 (49.06%), Query Frame = -3 
Query: 1465 ETSKILGLTNCYRKFIDNDTKYSKPLESLTSSQSRKLLGS----------------EECQKAFV*LKEKLCMAPILICTDLN*SFILDNDASFDRIGVVLDEK*KG 1734
            E    LG  + YR+F++  +K + PL  L      KL G+                E C+KAF  LKE+L  AP+L   D +  FI++ DAS   +G VL ++ +G
Sbjct:  552 ELRSFLGFASYYRRFVEGFSKLAAPLHQLVG----KLGGARRKGKTLPVPLAASWDERCEKAFQSLKERLTSAPVLAYADFSKPFIVEVDASHGGLGAVLSQEQEG 653
[Close]
BLAST of SMED30020016 vs. Ensembl Cavefish
Match: ENSAMXT00000034038.1 (pep primary_assembly:Astyanax_mexicanus-2.0:14:3120146:3124090:1 gene:ENSAMXG00000040885.1 transcript:ENSAMXT00000034038.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 99.7525 bits (247), Expect = 9.336e-32
Identity = 41/104 (39.42%), Postives = 68/104 (65.38%), Query Frame = -2 
Query: 1742 MPFGIAEAPAIFQRLMNKVLEEINWKIVIVYLDDIFICSRDYESHIRDIEHVFIKIEKAGLKISSSKCKFLRTEVRFLEHIINETGLKTDTSKIDSIKHFQTPQ 2053
            MPFG+  +P+ FQRLM     ++N++ +++YLDDI I SR ++ H+  ++ VF ++ K GLK+   KC  LR EV +L H+++  G+KTD  KI  +  ++ P+
Sbjct:  360 MPFGLQNSPSTFQRLMTCCFGDLNFESLLIYLDDIVIFSRTFDEHLERLQMVFDRLRKYGLKLKPQKCHLLRREVLYLGHVVSSDGIKTDPDKISKVAGWKVPE 463

HSP 2 Score: 57.7658 bits (138), Expect = 9.336e-32
Identity = 51/177 (28.81%), Postives = 77/177 (43.50%), Query Frame = -3 
Query: 1303 KCPETS----KILGLTNCYRKFIDNDTKYSKPLESLTSSQSRK--------------LLGSEECQKAFV*LKEKLCMAPILICTDLN*SFILDNDASFDRIGVVLDEK*KG*LHMGQKHSI---NINLNIALQEKFF*PHQF---------TVYFKHYLYGKRFKLRSNQKALTFMI 1743
            K PE      + LG    YR+FI+     + PL  LTS   RK               + +EEC+ AF  LK KL  AP+L   D +  F+L  DAS   +G VL +     +  G++  I   +  LN A  E  +  H+          T  F  +LYG +F + ++   L +++
Sbjct:  460 KVPENRHELLQFLGFAGYYRRFIEGYASIAAPLYRLTSGDPRKKKRGRKGPPAVGLPFIWTEECENAFQTLKTKLTTAPVLGYADYSQPFVLQTDASLAGLGAVLAQ-----VQDGRERVIAYASRGLNPA--ETRYPAHKLEFLALKWAVTDKFYDHLYGHKFSVLTDNNPLKYVM 629
[Close]
BLAST of SMED30020016 vs. Ensembl Cavefish
Match: ENSAMXT00000052546.1 (pep primary_assembly:Astyanax_mexicanus-2.0:2:13031363:13036570:1 gene:ENSAMXG00000033629.1 transcript:ENSAMXT00000052546.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 104.76 bits (260), Expect = 3.812e-31
Identity = 48/108 (44.44%), Postives = 72/108 (66.67%), Query Frame = -2 
Query: 1730 MPFGIAEAPAIFQRLMNKVLEEINWKIVIVYLDDIFICSRDYESHIRDIEHVFIKIEKAGLKISSSKCKFLRTEVRFLEHIINETGLKTDTSKIDSIKHFQTPQVSRN 2053
            MPFG+  APA FQRLM  VL ++ + I++VYLDDI I SRD++SH   +E VF ++   GLK+  SKC  L+ EV+FL HII+  G++ D  K+  ++ +  P+  ++
Sbjct:  844 MPFGLCNAPATFQRLMGVVLGDLAFDILLVYLDDIIIFSRDFKSHCERLELVFNRLRHHGLKLKPSKCFLLKPEVKFLGHIISAKGVQVDAEKVRVLETWPIPKTVKD 951

HSP 2 Score: 50.8322 bits (120), Expect = 3.812e-31
Identity = 34/101 (33.66%), Postives = 48/101 (47.52%), Query Frame = -3 
Query: 1465 PETSK----ILGLTNCYRKFIDNDTKYSKPLESLTSSQSRK------LLGSEECQKAFV*LKEKLCMAPILICTDLN*SFILDNDASFDRIGVVLDEK*KG 1737
            P+T K    +LG  + YR+F+    + +KPL +L   +  +      +  S ECQ AF  LK  L   P+L   D    FIL  D S   +G VL +K  G
Sbjct:  946 PKTVKDIRQVLGFMSYYRRFVPKFAQLAKPLHALVGGKMNRGRATGPITWSGECQTAFDKLKGCLMSPPVLAYPDFTQPFILTTDGSLHGLGAVLSQKQGG 1046
[Close]
BLAST of SMED30020016 vs. Ensembl Cavefish
Match: ENSAMXT00000048059.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02001896.1:45492:48938:1 gene:ENSAMXG00000034382.1 transcript:ENSAMXT00000048059.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 99.7525 bits (247), Expect = 6.337e-31
Identity = 38/104 (36.54%), Postives = 71/104 (68.27%), Query Frame = -2 
Query: 1742 MPFGIAEAPAIFQRLMNKVLEEINWKIVIVYLDDIFICSRDYESHIRDIEHVFIKIEKAGLKISSSKCKFLRTEVRFLEHIINETGLKTDTSKIDSIKHFQTPQ 2053
            MPFG+  APA FQRLM   L + N++ +++YLDD+ + + D+ SH+  ++ VF ++ + GLK+   KCK LR+ V++L H++++ G+  D  K+ +++ ++ P+
Sbjct:  262 MPFGLCNAPASFQRLMESCLGDQNYQSLLIYLDDVIVFAADFNSHLDRLDFVFSRLSQQGLKLKPDKCKLLRSSVQYLGHVVSDAGIAPDPDKVMAVQQWRVPK 365

HSP 2 Score: 55.0694 bits (131), Expect = 6.337e-31
Identity = 33/100 (33.00%), Postives = 44/100 (44.00%), Query Frame = -3 
Query: 1465 ETSKILGLTNCYRKFIDNDTKYSKPLESLTSSQSRK----------LLGSEECQKAFV*LKEKLCMAPILICTDLN*SFILDNDASFDRIGVVLDEK*KG 1734
            E    LGL   YR+F+   ++ + PL  L      K             S EC+ AF  LK+ L  AP+L   D    F+L  DAS   +G VL +K  G
Sbjct:  369 ELRAFLGLAGYYRRFVKGFSQIAAPLHRLLGGHPTKKSKAKALCPPWKWSNECETAFESLKQGLVQAPVLAFADFTLPFLLYTDASHRGLGAVLSQKQDG 468
[Close]
BLAST of SMED30020016 vs. Ensembl Nematostella
Match: EDO33875 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SR01])

HSP 1 Score: 46.595 bits (109), Expect = 9.656e-7
Identity = 23/71 (32.39%), Postives = 40/71 (56.34%), Query Frame = -2 
Query: 1745 DDIFICSRDYESHIRDIEHVFIKIEKAGLKISSSKCKFLRTEVRFLEHIINETGLKTDTSKIDSIKHFQTP 1957
            DD+      +E H+R IE +   +  +G K S  K +F    V+FL H+I++ G++    K+D I+ ++TP
Sbjct:    1 DDVICFHSSFEEHLRGIERMLQAVRASGFK-SIKKSQFATRSVKFLGHVIDQNGVRPQPEKLD-IRQWETP 69
[Close]
BLAST of SMED30020016 vs. Ensembl Medaka
Match: ENSORLT00000043031.1 (pep primary_assembly:ASM223467v1:7:14435445:14438777:-1 gene:ENSORLG00000023674.1 transcript:ENSORLT00000043031.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 87.8113 bits (216), Expect = 4.757e-30
Identity = 42/114 (36.84%), Postives = 67/114 (58.77%), Query Frame = -2 
Query: 1718 LMMPFGIAEAPAIFQRLMNKVLEEINWKIVIVYLDDIFICSRDYESHIRDIEHVFIKIEKAGLKISSSKCKFLRTEVRFLEHIINETGLKTDTSKIDSIKHFQTPQVSRNFEDF 2059
            L+MPFG+  APA+FQRL+N VL +   + V VYLDDI I S D   H   +  V  ++ +  L +   KC+F  T V+FL +I+    ++ D SKI+++ +++ P   +  + F
Sbjct:  334 LVMPFGLTNAPAVFQRLVNDVLRDFLNRFVFVYLDDILIYSNDQAQHEHHVRLVLERLLENQLYVKVEKCEFHVTTVQFLGYIVEAGCIRPDPSKIEAVANWEPPDNRKKLQQF 447

HSP 2 Score: 63.929 bits (154), Expect = 4.757e-30
Identity = 36/84 (42.86%), Postives = 49/84 (58.33%), Query Frame = -3 
Query: 1474 KILGLTNCYRKFIDNDTKYSKPLESLTSSQSRKLLGSEECQKAFV*LKEKLCMAPILICTDLN*SFILDNDASFDRIGVVLDEK 1725
            + LG  N YR+FI N +  + PL  LTS+ +R  L S E Q+AF  LK     APILI  D +  F+++ DAS   +G VL +K
Sbjct:  446 QFLGFANFYRRFIRNYSSIASPLTRLTST-NRPYLWSTEAQQAFDTLKRLFVSAPILIQPDPSRQFVVEVDASDSGVGAVLSQK 528
[Close]
BLAST of SMED30020016 vs. Ensembl Medaka
Match: ENSORLT00000034171.1 (pep primary_assembly:ASM223467v1:20:12459257:12462301:-1 gene:ENSORLG00000022683.1 transcript:ENSORLT00000034171.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 91.2781 bits (225), Expect = 2.756e-29
Identity = 40/114 (35.09%), Postives = 71/114 (62.28%), Query Frame = -2 
Query: 1718 LMMPFGIAEAPAIFQRLMNKVLEEINWKIVIVYLDDIFICSRDYESHIRDIEHVFIKIEKAGLKISSSKCKFLRTEVRFLEHIINETGLKTDTSKIDSIKHFQTPQVSRNFEDF 2059
            L+MPFG+  APA+FQ L+N VL+++  K V VYLDDI I S D ++H++ +  V  ++ +  L   + KC+F   + RFL H+++  G++ D++K+ ++  +  P   +  + F
Sbjct:  226 LVMPFGLTNAPAVFQCLINDVLKDMINKFVFVYLDDILIFSPDLDTHVQHVRTVLQRLLENQLYCKAEKCEFHTKQTRFLGHVVSPGGVQMDSAKVKAVLEWPVPSGRKQLQRF 339

HSP 2 Score: 58.151 bits (139), Expect = 2.756e-29
Identity = 31/87 (35.63%), Postives = 46/87 (52.87%), Query Frame = -3 
Query: 1474 ETSKILGLTNCYRKFIDNDTKYSKPLESLTSSQSRKLLGSEECQKAFV*LKEKLCMAPILICTDLN*SFILDNDASFDRIGVVLDEK 1734
            +  + LG  N YR+FI   +  + PL  LTS++ R  +  E   K F  LK +   APIL   D +  FI++ DAS   +G VL ++
Sbjct:  335 QLQRFLGFANFYRRFIRGFSGVAAPLHKLTSAKVR-FVWDEGADKTFTELKRRFSTAPILTQPDPSKQFIVEVDASESGVGAVLSQR 420
[Close]
BLAST of SMED30020016 vs. Ensembl Medaka
Match: ENSORLT00000042077.1 (pep primary_assembly:ASM223467v1:8:23690816:23694139:1 gene:ENSORLG00000027877.1 transcript:ENSORLT00000042077.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 85.8853 bits (211), Expect = 3.090e-29
Identity = 41/114 (35.96%), Postives = 67/114 (58.77%), Query Frame = -2 
Query: 1718 LMMPFGIAEAPAIFQRLMNKVLEEINWKIVIVYLDDIFICSRDYESHIRDIEHVFIKIEKAGLKISSSKCKFLRTEVRFLEHIINETGLKTDTSKIDSIKHFQTPQVSRNFEDF 2059
            L+MPFG+  APA+FQRL+N +L +     V V+LDDI I S+D   H   +  V  ++ +  L +   KC+F  T V+FL +II    ++ D SKI+++ +++ P   +  + F
Sbjct:  331 LVMPFGLTNAPAVFQRLVNDILRDFLNHFVFVHLDDILIYSKDQAQHEHHVRLVLERLLENQLFVKVEKCEFHVTTVQFLGYIIEAGCIRPDPSKIEAVANWEPPDSRKKLQQF 444

HSP 2 Score: 63.5438 bits (153), Expect = 3.090e-29
Identity = 36/84 (42.86%), Postives = 49/84 (58.33%), Query Frame = -3 
Query: 1474 KILGLTNCYRKFIDNDTKYSKPLESLTSSQSRKLLGSEECQKAFV*LKEKLCMAPILICTDLN*SFILDNDASFDRIGVVLDEK 1725
            + LG  N YR+FI N +  + PL  LTS+ +R  L S E Q+AF  LK     APILI  D +  F+++ DAS   +G VL +K
Sbjct:  443 QFLGFANFYRRFIRNYSSIASPLTRLTST-NRPYLWSTEAQQAFDKLKGLFVTAPILIRPDPSSQFVVEVDASDSGVGAVLSQK 525
[Close]
BLAST of SMED30020016 vs. Ensembl Medaka
Match: ENSORLT00000036564.1 (pep primary_assembly:ASM223467v1:16:11419237:11425467:1 gene:ENSORLG00000022696.1 transcript:ENSORLT00000036564.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 89.3521 bits (220), Expect = 7.254e-29
Identity = 42/114 (36.84%), Postives = 67/114 (58.77%), Query Frame = -2 
Query: 1718 LMMPFGIAEAPAIFQRLMNKVLEEINWKIVIVYLDDIFICSRDYESHIRDIEHVFIKIEKAGLKISSSKCKFLRTEVRFLEHIINETGLKTDTSKIDSIKHFQTPQVSRNFEDF 2059
            L+MPFG+  APA+FQRL+N VL +   + V VYLDDI I S +   H + +  V  ++ +  L + + KC+F    V+FL +II    +K D SKI+++  ++ P   +  + F
Sbjct:  661 LVMPFGLTNAPAVFQRLVNNVLRDFLNRFVFVYLDDILIYSNNLTQHEQHVRQVLTRLLENQLYVKAEKCEFHVKSVQFLGYIIEAGRIKPDPSKIEAVSAWEPPTNRKKLQQF 774

HSP 2 Score: 58.5362 bits (140), Expect = 7.254e-29
Identity = 34/84 (40.48%), Postives = 49/84 (58.33%), Query Frame = -3 
Query: 1474 KILGLTNCYRKFIDNDTKYSKPLESLTSSQSRKLLGSEECQKAFV*LKEKLCMAPILICTDLN*SFILDNDASFDRIGVVLDEK 1725
            + LG  N YR+FI N +  + PL  LTS+  +    ++E Q+AF  LK+    APILI  D +  FI++ DAS   +G VL +K
Sbjct:  773 QFLGFANFYRRFIRNYSSIAAPLTRLTSTH-QNFQWTQEAQQAFDHLKKLFVTAPILIQPDPSRQFIVEVDASDSGVGAVLSQK 855
[Close]
BLAST of SMED30020016 vs. Ensembl Medaka
Match: ENSORLT00000041674.1 (pep primary_assembly:ASM223467v1:5:30088905:30092924:1 gene:ENSORLG00000022054.1 transcript:ENSORLT00000041674.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 92.0485 bits (227), Expect = 7.756e-29
Identity = 39/112 (34.82%), Postives = 66/112 (58.93%), Query Frame = -2 
Query: 1718 MPFGIAEAPAIFQRLMNKVLEEINWKIVIVYLDDIFICSRDYESHIRDIEHVFIKIEKAGLKISSSKCKFLRTEVRFLEHIINETGLKTDTSKIDSIKHFQTPQVSRNFEDF 2053
            MPFG+  AP  FQRLM ++  +   + V++YLDDI + S   + H+  +E V  ++++  L++   KC F + EVR+L H+I++ G+ TD  KI+++  +Q P        F
Sbjct:  435 MPFGLCNAPGTFQRLMQRIFGDQQCQSVLLYLDDIVVFSSTIDEHLERLELVLGRLQQEKLRVKLPKCAFFQQEVRYLGHVISDQGVSTDPHKIEAVAGWQPPSTVSELRTF 546

HSP 2 Score: 55.4546 bits (132), Expect = 7.756e-29
Identity = 37/101 (36.63%), Postives = 53/101 (52.48%), Query Frame = -3 
Query: 1465 ETSKILGLTNCYRKFIDNDTKYSKPLESL------TSSQSRK---LLG--SEECQKAFV*LKEKLCMAPILICTDLN*SFILDNDASFDRIGVVLDEK*KG 1734
            E    LG  + YR+F++   + + PL  L      T S+ RK   L G  + ECQ+ F  LK+KL  AP+L   D    FIL+ DAS + +G VL ++  G
Sbjct:  542 ELRTFLGFASYYRRFVEGFARLAAPLHRLVGELDGTKSRRRKASSLQGHWTTECQQNFDALKQKLTSAPVLAYADFTLPFILEVDASHNGLGAVLSQEQGG 642
[Close]
BLAST of SMED30020016 vs. Planmine SMEST
Match: SMESG000031172.1 (SMESG000031172.1)

HSP 1 Score: 98.9821 bits (245), Expect = 1.424e-40
Identity = 49/104 (47.12%), Postives = 65/104 (62.50%), Query Frame = -2 
Query: 1742 MPFGIAEAPAIFQRLMNKVLEEINWKIVIVYLDDIFICSRDYESHIRDIEHVFIKIEKAGLKISSSKCKFLRTEVRFLEHIINETGLKTDTSKIDSIKHFQTPQ 2053
            MPF IA AP  F+ LMNKVL  +NWK  +VYLDDI   SR                 KAGLK+S  KC FLR + +FL HIIN+ G++T+ +KI++IK ++ P+
Sbjct:  293 MPFEIAAAPTTFKNLMNKVLGTLNWKETVVYLDDILSFSR-----------------KAGLKLSKDKCHFLRKQTKFLGHIINKDGIRTEDNKIEAIKEYERPK 379

HSP 2 Score: 87.8113 bits (216), Expect = 1.424e-40
Identity = 49/98 (50.00%), Postives = 64/98 (65.31%), Query Frame = -3 
Query: 1465 FKHPKCPETSKI-LGLTNCYRKFIDNDTKYSKPLESLTSSQSRKLLGSEECQKAFV*LKEKLCMAPILICTDLN*SFILDNDASFDRIGVVLDEK*KG 1755
            ++ PKC +  +I L +TN YR+F  N T+Y+KPLE L  S S KL+ ++ C+ AF  LKEKL  APIL   D +  FILD DASFDRI  VL ++ K 
Sbjct:  375 YERPKCIKKLRIFLSMTNYYRRFNKNYTEYTKPLEILCGSNSNKLIVTDICESAFQSLKEKLMKAPILAYLDFSKEFILDTDASFDRIKAVLSQEDKN 472
[Close]
BLAST of SMED30020016 vs. Planmine SMEST
Match: SMESG000061863.1 (SMESG000061863.1)

HSP 1 Score: 124.02 bits (310), Expect = 2.055e-40
Identity = 60/101 (59.41%), Postives = 74/101 (73.27%), Query Frame = -2 
Query: 1751 MPFGIAEAPAIFQRLMNKVLEEINWKIVIVYLDDIFICSRDYESHIRDIEHVFIKIEKAGLKISSSKCKFLRTEVRFLEHIINETGLKTDTSKIDSIKHFQ 2053
            MPFGIA  PAIFQ+LMN VL E+NWK  IVYLDDI I S++   H ++I  V  KI  AGLKIS SKC+FL+ EV+ L H IN+ G+KTD +KI +IK F+
Sbjct:    1 MPFGIAAVPAIFQKLMNIVLGELNWKFAIVYLDDILIFSKELNQHYKNIRSVLEKIRLAGLKISPSKCQFLKDEVKILGHTINKIGVKTDENKIQAIKQFE 101

HSP 2 Score: 49.6766 bits (117), Expect = 2.055e-40
Identity = 26/58 (44.83%), Postives = 37/58 (63.79%), Query Frame = -3 
Query: 1483 ESLTSSQSRKLLGSEECQKAFV*LKEKLCMAPILICTDLN*SFILDNDASFDRIGVVL 1656
            + L   + +KL+ +E+ +KAF+   +KL  APIL  +D    FILD + SFDRIG VL
Sbjct:  111 KKLCGGKEKKLIWTEDGEKAFIEHSDKLINAPILKFSDFLRPFILDTNDSFDRIGAVL 168

HSP 3 Score: 33.113 bits (74), Expect = 2.055e-40
Identity = 16/26 (61.54%), Postives = 17/26 (65.38%), Query Frame = -2 
Query: 1388 VIAYGSKAFNKH*FEYCITRKIFLAT 1465
            VIAYGS A +K    YCITRK  LA 
Sbjct:  179 VIAYGSHAMSKQEVGYCITRKELLAN 204
[Close]
BLAST of SMED30020016 vs. Planmine SMEST
Match: SMESG000081257.1 (SMESG000081257.1)

HSP 1 Score: 93.5893 bits (231), Expect = 2.416e-40
Identity = 42/112 (37.50%), Postives = 73/112 (65.18%), Query Frame = -2 
Query: 1718 MPFGIAEAPAIFQRLMNKVLEEINWKIVIVYLDDIFICSRDYESHIRDIEHVFIKIEKAGLKISSSKCKFLRTEVRFLEHIINETGLKTDTSKIDSIKHFQTPQVSRNFEDF 2053
            +PFG++ APA FQ+LM+++LE + +K V+VYLDDI I   + E+H + +  VF +I  +GLK++  KC F ++ + F+ H ++E G++T+  KI  I++   P+ +     F
Sbjct:  574 LPFGLSTAPATFQKLMHQILEGLVFKGVVVYLDDILIYGENQETHDKLLFEVFTRIRDSGLKVNPEKCAFNKSVLNFIGHTVSEKGIQTNKRKISEIENATEPKSTTELRSF 685

HSP 2 Score: 92.4337 bits (228), Expect = 2.416e-40
Identity = 55/143 (38.46%), Postives = 80/143 (55.94%), Query Frame = -3 
Query: 1306 ETSKILGLTNCYRKFIDNDTKYSKPLESLTSSQSRKLLGSEECQKAFV*LKEKLCMAPILICTDLN*SFILDNDASFDRIGVVLDEK*KG*LHMGQKHSININLNIALQEKFF*PHQFTVYFKHYLYGKRFKLRSNQKALTFM 1734
            E    LGLT  YR+FI N +  + PL + T+   + ++ +EEC+K F+ LK+ LC APIL     +  FI+D D SF+ IG           H+  KH I        +++    H+F V+F+ YLYG+RF  R++ KALTFM
Sbjct:  681 ELRSFLGLTTYYRRFIKNYSMIAAPLYAATTGCDKMIVWTEECRKRFINLKKLLCEAPILEYPRADRLFIIDTDDSFEAIG-----------HL-TKHEIGY---CVTRKELLVLHEFIVHFRQYLYGRRFVARTDHKALTFM 808
[Close]
BLAST of SMED30020016 vs. Planmine SMEST
Match: SMESG000029259.1 (SMESG000029259.1)

HSP 1 Score: 106.301 bits (264), Expect = 5.217e-37
Identity = 63/164 (38.41%), Postives = 91/164 (55.49%), Query Frame = -3 
Query: 1294 FKHPKCPETSK-ILGLTNCYRKFIDNDTKYSKPLESLTSSQSRKLLGSEECQKAFV*LKEKLCMAPILICTDLN*SFILDNDASFDRIGVVLDEK*KG*LHMGQKHSININLNIA---------LQEKFF*PHQFTVYFKHYLYGKRFKLRSNQKALTFMILNK 1755
            F+ PKC +  +  LG+ N YR+FI +  K ++ LES+    + K+  +E C+KAF  +KE L  AP+L+  D    FILD DASFD IG VL +K       G +H I    +            +++    + F  +F HYLYGKRF LR++ KA+TFM+  K
Sbjct: 2949 FQKPKCVKNLRSFLGICNYYRRFIKDYAKKARALESICGKNNEKIRWTEMCEKAFGEMKEALITAPVLVFPDFRKEFILDTDASFDTIGAVLSQK----DEKGHEHVIAYGSHAMSSHEKGYCITRKELLAIYYFCKHFNHYLYGKRFVLRTDHKAITFMVTTK 3108

HSP 2 Score: 68.5514 bits (166), Expect = 5.217e-37
Identity = 62/194 (31.96%), Postives = 96/194 (49.48%), Query Frame = -1 
Query:  666 CITCTRCQTIHREAKID*LKTILIATMTENLFDKWPKDSNEIKPIN-----------LVAQQIQNLKLKIIL*SDRR*NLDVR*K*KPIYR*IS*EIMPCR*Q*SRTYTCHNFTMDNLSTKVKEVIERCENCQKRKTYTIRTKKPIVFLEDSKIFEVVYTDLLVRSP---TL*EKRYILGIIDHKSKYIVLKEI 1205
            C TC +C   H +AK   +KT ++    E  ++KW  D+ E++ I            +    +   + KI + SD R         + + + +   +     Q    Y  +N  M+NL+T+VK+VIE CE CQK KT T +TK+    ++ ++ FE +Y D+    P   T  +K+YI GIIDH SKYI L  I
Sbjct: 3148 CGTCVQCMMEHEDAKTGKIKTRILTVTAEGGYNKWQNDNMEVQEIKNKLENKDCKFIMENNTVLTKQGKIWIPSDNR---------QRMIKEVHVLLCHAGAQKVTKYIQNNCDMENLATEVKKVIENCERCQKMKTITTKTKEETQTIKSTEPFEKIYMDIC--GPLKETFNKKKYICGIIDHYSKYISLTAI 3330

HSP 3 Score: 110.538 bits (275), Expect = 2.051e-24
Identity = 55/113 (48.67%), Postives = 75/113 (66.37%), Query Frame = -2 
Query: 1718 MPFGIAEAPAIFQRLMNKVLEEINWK-IVIVYLDDIFICSRDYESHIRDIEHVFIKIEKAGLKISSSKCKFLRTEVRFLEHIINETGLKTDTSKIDSIKHFQTPQVSRNFEDF 2053
            MPFGIA AP  FQ LM KVL+++ WK  V+VYLDDI I ++  E H      V  KI  AGL+++  KC+  R EV+FL HIIN+ G++TD +KI++I+ FQ P+  +N   F
Sbjct: 2850 MPFGIAAAPGTFQELMTKVLKDL-WKDGVMVYLDDILIFTKTEEDHYNIFGKVLGKIATAGLRLNPEKCQIFRKEVKFLGHIINKDGIQTDNTKIEAIQSFQKPKCVKNLRSF 2961

HSP 4 Score: 53.1434 bits (126), Expect = 7.886e-7
Identity = 34/84 (40.48%), Postives = 44/84 (52.38%), Query Frame = -2 
Query: 1214 VIAYGSKAFNKH*FEYCITRKIFLATSIYSLL*ALSVWKTIQT*IKPKSSYIYDIKQRKSITTQFQTRMNYLSS*DIEFEYRIG 1465
            VIAYGS A + H   YCITRK  LA   +       ++           +  + +  +K IT QFQT +NYLSS DI+ EYR G
Sbjct: 3051 VIAYGSHAMSSHEKGYCITRKELLAIYYFCKHFNHYLYGKRFVLRTDHKAITFMVTTKKPITAQFQTWINYLSSLDIKMEYRKG 3134
[Close]
BLAST of SMED30020016 vs. Planmine SMEST
Match: SMESG000029866.1 (SMESG000029866.1)

HSP 1 Score: 144.436 bits (363), Expect = 7.071e-37
Identity = 89/173 (51.45%), Postives = 105/173 (60.69%), Query Frame = -1 
Query:  693 SVCITCTRCQTIHREAKID*LKTILIATMTENLFDKWPKDSNEIKPINLVAQQIQNLKLKIIL*SDRR*NLDVR*K*KPIYR*IS*EIMPCR*Q*SRTYTCHNFTMDNLSTKVKEVIERCENCQKRKTYTIRTKKPIVFLEDSKIFEVVYTDLLVRSPTL*EKRYILGIIDHK 1211
            S C+TCT+CQTIH EAKI  LKT LIATMTENLFDKW  D NEIK I   +    N K  +          ++  K + I +                Y CHN  M+NLS+KVKEVIE+CENC+KRKTYT RTK+PIV  ED KIFEV+Y D+         K+YILGIID K
Sbjct:  322 SDCVTCTQCQTIHTEAKIGKLKTKLIATMTENLFDKWQNDINEIKTIK--SSDSTNSKFNV--------ENNLVKKIEGIKK-------------VEKYICHNIAMNNLSSKVKEVIEKCENCEKRKTYTNRTKEPIVSQEDYKIFEVIYIDIY-------GKKYILGIIDQK 464

HSP 2 Score: 30.0314 bits (66), Expect = 7.071e-37
Identity = 23/73 (31.51%), Postives = 33/73 (45.21%), Query Frame = -2 
Query: 1208 FEYCITRKIFLATSIYSLL*ALSVWKTIQT*IKPKSSYIYDIKQRKSITTQFQTRMNYLSS*DIEFEYRIGEV 1426
            F YCITRK  LA   +++                   Y+Y  +           ++++LSS DIEFEYR GE+
Sbjct:  265 FGYCITRKELLAAYHFTV---------------HFKQYLYGKR----------FKLSHLSSLDIEFEYRKGEI 312

HSP 3 Score: 76.2554 bits (186), Expect = 3.334e-14
Identity = 43/79 (54.43%), Postives = 48/79 (60.76%), Query Frame = -2 
Query: 1724 LDDIFICSRDYESHIRDIEHVFIKIEKAGLKISSSKCKFLRTEVRFLEHIINETGLKTDTSKIDSIKHFQTPQVSRNFE 1960
             DDI I S DYESHI+DIEHVFIKIEKAGLKIS S CKFLRTE +                    ++ F+   VSRNFE
Sbjct:  204 FDDILIFSGDYESHIKDIEHVFIKIEKAGLKISPSNCKFLRTETQ--------------------LRIFKRTNVSRNFE 262
The following BLAST results are available for this feature:
BLAST of SMED30020016 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020016 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020016 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020016 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
BX511224.14.809e-2835.40pep chromosome:GRCz11:2:18017000:18022765:1 gene:E... [more]
BX546500.13.021e-2734.51pep chromosome:GRCz11:23:12926092:12931693:-1 gene... [more]
CR855320.16.077e-2735.40pep chromosome:GRCz11:1:7956030:7961696:1 gene:ENS... [more]
FO704673.11.579e-2632.46pep chromosome:GRCz11:12:14545475:14551077:-1 gene... [more]
BX511082.13.811e-2633.63pep chromosome:GRCz11:9:14291932:14297132:1 gene:E... [more]
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BLAST of SMED30020016 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSXETT00000006041.11.698e-2536.61pep primary_assembly:Xenopus_tropicalis_v9.1:6:373... [more]
anxa69.152e-1835.40annexin A6 [Source:Xenbase;Acc:XB-GENE-989741][more]
denr3.560e-1734.21density-regulated protein [Source:Xenbase;Acc:XB-G... [more]
castor14.064e-1734.21cytosolic arginine sensor for mTORC1 subunit 1 [So... [more]
ENSXETT00000020886.14.064e-1734.21pep primary_assembly:Xenopus_tropicalis_v9.1:3:496... [more]
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BLAST of SMED30020016 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020016 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P20825|POL2_DROME1.923e-2740.35Retrovirus-related Pol polyprotein from transposon... [more]
sp|Q8I7P9|POL5_DROME1.906e-2536.84Retrovirus-related Pol polyprotein from transposon... [more]
sp|P10394|POL4_DROME1.020e-1931.07Retrovirus-related Pol polyprotein from transposon... [more]
sp|P04323|POL3_DROME1.097e-1839.47Retrovirus-related Pol polyprotein from transposon... [more]
sp|P0CT39|TF26_SCHPO1.670e-1433.33Transposon Tf2-6 polyprotein OS=Schizosaccharomyce... [more]
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BLAST of SMED30020016 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A087SUZ93.079e-3538.05Retrovirus-related Pol polyprotein from transposon... [more]
A0A4Y2EP604.791e-3541.07Transposon Ty3-G Gag-Pol polyprotein OS=Araneus ve... [more]
A0A087T2931.286e-3446.15Retrovirus-related Pol polyprotein from transposon... [more]
A0A4Y2EIJ51.798e-3442.48Retrovirus-related Pol polyprotein from transposon... [more]
A0A4Y2DW632.196e-3441.59Retrovirus-related Pol polyprotein from transposon... [more]
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BLAST of SMED30020016 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSAMXT00000038088.12.606e-3441.07pep primary_assembly:Astyanax_mexicanus-2.0:23:138... [more]
ENSAMXT00000037150.15.604e-3238.83pep primary_assembly:Astyanax_mexicanus-2.0:10:222... [more]
ENSAMXT00000034038.19.336e-3239.42pep primary_assembly:Astyanax_mexicanus-2.0:14:312... [more]
ENSAMXT00000052546.13.812e-3144.44pep primary_assembly:Astyanax_mexicanus-2.0:2:1303... [more]
ENSAMXT00000048059.16.337e-3136.54pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
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BLAST of SMED30020016 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020016 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30020016 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO338759.656e-732.39Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SMED30020016 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSORLT00000043031.14.757e-3036.84pep primary_assembly:ASM223467v1:7:14435445:144387... [more]
ENSORLT00000034171.12.756e-2935.09pep primary_assembly:ASM223467v1:20:12459257:12462... [more]
ENSORLT00000042077.13.090e-2935.96pep primary_assembly:ASM223467v1:8:23690816:236941... [more]
ENSORLT00000036564.17.254e-2936.84pep primary_assembly:ASM223467v1:16:11419237:11425... [more]
ENSORLT00000041674.17.756e-2934.82pep primary_assembly:ASM223467v1:5:30088905:300929... [more]
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BLAST of SMED30020016 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000031172.11.424e-4047.12SMESG000031172.1[more]
SMESG000061863.12.055e-4059.41SMESG000061863.1[more]
SMESG000081257.12.416e-4037.50SMESG000081257.1[more]
SMESG000029259.15.217e-3738.41SMESG000029259.1[more]
SMESG000029866.17.071e-3751.45SMESG000029866.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30020016 ID=SMED30020016|Name=SMED30020016|organism=Schmidtea mediterranea sexual|type=transcript|length=2111bp
AAATAATTACTCAAATTCATAATTCACAGTTTATATCACATAAAATTAAA
CCTAAAATCAACAAACTAAAAAATTAAATTTATTTTAAAATATGACTGCA
ATGTTTTTTTTCATTAATTTGGTTTGAAGAGCAACGACGAAATTCTCATG
GTTGTTCAGTAAAAAGAATTTAATCTCATTGATATATGAAAGATGCTGAC
TCGGATATTTGCCTTTAATTAGATTTACGTATGATATTTCAGGTTATTTT
GAAAACAATCTTCGTGTGATATTTAAAAAATCAATTCTACATGATATAGT
TGAGTTATGACCATAAGGAAATGTATTTGAGTAATTATTTGACTGGTGAA
TTTGTTGATCCTGACTTTGGTTTGAAGGGCTTATGATTTGCAGAATTGTC
AATCGGATTTGTATAGATTTCGATGTTGCAACCTGGGGAACTCACCACAG
CGACATTTGTATCCAGGTTTCATTGCCTATCTTAATTTCTCTATCACACC
ACTTCATTTTATTTCCCCATTACCATCAATTCATTGTCTATCTCATCTGG
AGATGTCTATTTGTTTTCTGGTGTTTTTGAACATTTACCCTCCAAATATA
CTGCTTTGATCAATTATTTATTAACTAACTAGTCGCATTATATACATTCT
GAGATATACATTTAATATTTCTTTTAGTACTATATATTTACTTTTGTGAT
CAATAATTCCTAAAATATATCTCTTTTCCTACAGAGTTGGGGAACGGACC
AATAAATCCGTGTAAACTACTTCGAATATTTTAGAATCTTCTAGAAACAC
TATTGGTTTTTTCGTTCTAATAGTATACGTCTTTCGCTTTTGACAATTTT
CACATCTTTCTATGACTTCTTTTACTTTGGTGCTAAGATTATCCATTGTG
AAGTTGTGACATGTGTATGTTCTACTTTATTGCTACCTGCATGGCATAAT
TTCTTATGATATTCATCGATAGATGGGTTTCTACTTTCATCTGACATCCA
AATTTTACCTTCGATCGCTTTACAATATTATTTTCAACTTTAAATTTTGA
ATTTGTTGAGCCACTAGATTTATTGGTTTTATTTCATTACTGTCTTTTGG
CCATTTATCAAATAGATTTTCTGTCATTGTAGCAATTAATATTGTTTTCA
GTTAATCAATCTTTGCTTCTCGATGAATTGTTTGACACCGAGTACACGTT
ATACAAACACTTCACCTATTCTATATTCAAATTCAATATCCTAGCTACTA
AGATAGTTCATCCGAGTTTGAAATTGTGTGGTTATTGATTTTCTTTGTTT
AATATCATAAATGTAAGAGCTTTTTGGTTTGATCTAAGTTTGAATCGTTT
TCCATACAGATAATGCTTAAAGTAAACTGTAAATTGATGTGGCTAAAAAA
ATTTTTCTTGTAATGCAATATTCAAATTAATGTTTATTGAATGCTTTTGA
CCCATATGCAATTACCCTTTTCATTTTTCATCTAATACAACACCAATTCT
ATCAAAACTGGCATCATTATCCAAAATAAAACTTTAATTTAAGTCTGTAC
ATATTAGAATTGGCGCCATGCAAAGTTTCTCTTTTAGTTATACGAACGCT
TTCTGGCACTCTTCTGACCCTAATAATTTTCTACTTTGGCTGCTTGTCAA
CGATTCCAAGGGTTTTGAATACTTTGTGTCATTGTCTATAAATTTCCGGT
AACAATTTGTTAACCCTAAAATCTTCGAAGTTTCTGGACACTTGGGGTGT
TTGAAAATGCTTAATTGAGTCTATTTTAGAAGTATCAGTTTTTAAACCTG
TTTCATTTATAATATGTTCTAAAAACCTCACTTCTGTCCTTAGGAATTTG
CATTTTGAAGAGCTAATTTTTAATCCAGCTTTCTCTATTTTTATGAAAAC
ATGTTCTATGTCTCTGATGTGTGATTCATAATCCCTTGAGCAAATAAATA
TATCGTCTAAGTATACTATAACAATTTTCCAATTTATCTCTTCTAATACT
TTGTTCATCAGTCTTTGAAAAATTGCTGGTGCTTCGGCTATACCAAAAGG
CATCATTAAATTTCATGATTTCACTCGTTACACTGAGGGGACTTTCATCA
TTAATGACTAG
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protein sequence of SMED30020016-orf-1

>SMED30020016-orf-1 ID=SMED30020016-orf-1|Name=SMED30020016-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=126bp
MMPFGIAEAPAIFQRLMNKVLEEINWKIVIVYLDDIFICSRDYESHIRDI
EHVFIKIEKAGLKISSSKCKFLRTEVRFLEHIINETGLKTDTSKIDSIKH
FQTPQVSRNFEDFRVNKLLPEIYRQ*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: INTERPRO
TermDefinition
IPR000477RT_dom