SMED30019976
Overview
Neoblast Clusters
Zeng et. al., 2018
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny AppEmbryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30019976 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 2
Alignments
SMED30019976 aligns in the following genomic locations:
Homology
BLAST of SMED30019976 vs. Ensembl Zebrafish
Match: BX914205.2 (pep chromosome:GRCz11:4:64121772:64123545:-1 gene:ENSDARG00000099590.2 transcript:ENSDART00000170040.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:BX914205.2) HSP 1 Score: 73.559 bits (179), Expect = 4.961e-14 Identity = 57/181 (31.49%), Postives = 86/181 (47.51%), Query Frame = -2 Query: 488 KASARYEKEFWPELEQDIKKWIIKERTKCRQVSM**FRSXXXXXXXXXXXXXXXXGDHDS*TDTNYRF--------------ARQLPLD--KDFQLIRKK-NRR*KKTGIKAQHIVNIDKVLVSFNMIRNHTIDYKGAKEVKIISTDHEKSNFTIILCAIRGGGKCTPIVNLKRKIFLPNE 979 K S R K WP+LE +++WII++RT R VS + + + +RF A+QLP D + + R + + I+ +HI N+D+V ++F++ NHT++ KGA V I +T HEKS FT++L G K P+V KRK LP E Sbjct: 57 KQSFRGNKARWPQLEDQLEQWIIEQRTAGRSVS-----TVTIRLRATKIAQDMGIEHFQGGPSWCFRFMKRRHLSIRARTTVAQQLPADYKEKLDIFRTYCSNKITDKKIQPKHITNMDEVPLTFDIPVNHTVEKKGASTVSIRTTGHEKSAFTVVLGCHGDGQKLPPMVIFKRKT-LPKE 231
BLAST of SMED30019976 vs. Ensembl Zebrafish
Match: CR352209.1 (pep chromosome:GRCz11:24:26088143:26089609:1 gene:ENSDARG00000103972.2 transcript:ENSDART00000171129.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CR352209.1) HSP 1 Score: 73.1738 bits (178), Expect = 7.483e-14 Identity = 57/181 (31.49%), Postives = 86/181 (47.51%), Query Frame = -2 Query: 488 KASARYEKEFWPELEQDIKKWIIKERTKCRQVSM**FRSXXXXXXXXXXXXXXXXGDHDS*TDTNYRF--------------ARQLPLD--KDFQLIRKK-NRR*KKTGIKAQHIVNIDKVLVSFNMIRNHTIDYKGAKEVKIISTDHEKSNFTIILCAIRGGGKCTPIVNLKRKIFLPNE 979 K S R K WP+LE +++WII++RT R VS + + + +RF A+QLP D + + R + + I+ +HI N+D+V ++F++ NHT++ KGA V I +T HEKS FT++L G K P+V KRK LP E Sbjct: 57 KQSFRGNKARWPQLEDQLEQWIIEQRTAGRSVS-----TVTIRLRATKIAQDMGIEHFQGGPSWCFRFMKRRHLSIRARTTVAQQLPADYKEKLDIFRTYCSNKITDKKIQPKHITNMDEVPLTFDIPVNHTVEKKGASTVSIRTTGHEKSAFTVVLGCHGDGQKLPPMVIFKRKT-LPKE 231
BLAST of SMED30019976 vs. Ensembl Zebrafish
Match: CR354398.1 (pep chromosome:GRCz11:7:64150332:64152087:-1 gene:ENSDARG00000101994.2 transcript:ENSDART00000165234.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CR354398.1) HSP 1 Score: 73.1738 bits (178), Expect = 8.407e-14 Identity = 57/181 (31.49%), Postives = 86/181 (47.51%), Query Frame = -2 Query: 488 KASARYEKEFWPELEQDIKKWIIKERTKCRQVSM**FRSXXXXXXXXXXXXXXXXGDHDS*TDTNYRF--------------ARQLPLD--KDFQLIRKK-NRR*KKTGIKAQHIVNIDKVLVSFNMIRNHTIDYKGAKEVKIISTDHEKSNFTIILCAIRGGGKCTPIVNLKRKIFLPNE 979 K S R K WP+LE +++WII++RT R VS + + + +RF A+QLP D + + R + + I+ +HI N+D+V ++F++ NHT++ KGA V I +T HEKS FT++L G K P+V KRK LP E Sbjct: 57 KQSFRGNKARWPQLEDQLEQWIIEQRTAGRSVS-----TVTIRLRATKIAQDMGIEHFQGGPSWCFRFMKRRHLSIRARTTVAQQLPADYKEKLDIFRTYCSNKITDKKIQPKHITNMDEVPLTFDIPVNHTVEKKGASTVSIRTTGHEKSAFTVVLGCHGDGQKLPPMVIFKRKT-LPKE 231
BLAST of SMED30019976 vs. TrEMBL
Match: A0A3P8Q0X0 (Uncharacterized protein OS=Astatotilapia calliptera OX=8154 PE=4 SV=1) HSP 1 Score: 80.4925 bits (197), Expect = 5.987e-13 Identity = 76/250 (30.40%), Postives = 123/250 (49.20%), Query Frame = -2 Query: 242 SARYEKEFWPELEQDIKKWIIKERTKCRQVSM**FRSXXXXXXXXXXXXXXXXGDHDS*TDTNYRFARQLPLDKDFQLIRKKNRR*KKTG---IKAQHIVNIDKVLVSFNMIRNHTIDYKGAKEVKIISTDHEKSNFTIILCAIRGGGKCTPIVNLKRKIFLPNEFXXXXXXXXXXXVWLIKKSNVSGLKWSGVCEKMLSFKNIAKSFLIWDFVTPRVVEDI*NLFVFGSY*KWVLK---SKDLQLRDYG 973 S R K WPELE+ +++WII++RT R +S R K +L + H S T A+QLP D +L ++ K G I+ HI N+D+V ++F++ +HT++ +G V I +T HEKS+FT++L G K P+V KRK +F +++K N W+ ++ L+ V + F + + S LI D + + D+ L + V+ +K+LQL D G Sbjct: 57 SFRGHKARWPELEERLERWIIEQRTSGRSISTVTIR------LKAVSLSLFVKLCHFS-IRTRTTVAQQLPADYKEKLAIFRSYCSKHIGDKNIQPSHITNMDEVPLTFDIPVSHTVEKQGTSTVAICTTGHEKSSFTVVLGCHANGQKLPPMVIFKRKTLPKEKFPAGIIIKANEKGWMDEEMMKEWLREMYV-RRPGGFFHASPSLLICDSMRAHLTADVKKLVKQMNCELAVIPGGLTKELQLLDIG 298
BLAST of SMED30019976 vs. TrEMBL
Match: A0A3P8QU07 (Uncharacterized protein OS=Astatotilapia calliptera OX=8154 PE=4 SV=1) HSP 1 Score: 79.7221 bits (195), Expect = 1.040e-12 Identity = 58/165 (35.15%), Postives = 83/165 (50.30%), Query Frame = -2 Query: 488 SARYEKEFWPELEQDIKKWIIKERTKCRQVSM**FRSXXXXXXXXXXXXXXXXGDHDS*TDTNYRFARQLPLDKDFQLIRKKNRR*KKTG---IKAQHIVNIDKVLVSFNMIRNHTIDYKGAKEVKIISTDHEKSNFTIILCAIRGGGKCTPIVNLKRKIFLPNE 973 S R K WPELE+ +++WII++RT R VS R K T A+QLP D +L ++ K G I+ HI N+D+V ++F++ +HT++ KG V I +T HEKS+FT++L G K P+V KRK LP E Sbjct: 57 SFRGHKARWPELEERLERWIIEQRTSGRSVSTVTIRLKAVSLSLFMKRCHFSIR-------TRTTVAQQLPADYKEKLAILRSYCSKHIGDKNIQPSHITNMDEVPLTFDIPVSHTVEKKGTSMVAIRTTGHEKSSFTVVLGCHANGQKLPPMVIFKRKT-LPKE 213
BLAST of SMED30019976 vs. TrEMBL
Match: A0A3P9AVH5 (Uncharacterized protein OS=Maylandia zebra OX=106582 PE=4 SV=1) HSP 1 Score: 79.337 bits (194), Expect = 1.138e-12 Identity = 61/165 (36.97%), Postives = 88/165 (53.33%), Query Frame = -2 Query: 488 SARYEKEFWPELEQDIKKWIIKERTKCRQVSM**FRSXXXXXXXXXXXXXXXXGDHDS*TDTNYRFARQLPLDKDFQLIRKKNRR*KKTG---IKAQHIVNIDKVLVSFNMIRNHTIDYKGAKEVKIISTDHEKSNFTIILCAIRGGGKCTPIVNLKRKIFLPNE 973 S R K WPELE+ +++WII++RT R VS R K +L + H S T A+QLP D +L ++ K G I+ HI N+D+V ++F++ +HT++ KG V I +T HEKS+FT++L G K P+V KRK LP E Sbjct: 57 SFRGHKARWPELEERLERWIIEQRTSGRSVSTVTIR------LKAVSLSLFMKRCHFS-IRTRTTVAQQLPADYKEKLAIFRSYCSKHIGDKNIQPSHITNMDEVPLTFDIPVSHTVEKKGTSTVAIRTTGHEKSSFTVVLDCHANGQKLPPMVIFKRKT-LPKE 213
BLAST of SMED30019976 vs. TrEMBL
Match: A0A2G9UMS9 (DDE superfamily endonuclease OS=Teladorsagia circumcincta OX=45464 GN=TELCIR_07348 PE=4 SV=1) HSP 1 Score: 78.9518 bits (193), Expect = 1.809e-12 Identity = 60/181 (33.15%), Postives = 90/181 (49.72%), Query Frame = -2 Query: 485 SKKASARYEKEFWPELEQDIKKWIIKERTKCRQVSM**FRSXXXXXXXXXXXXXXXXGDHDS*T-----DTNYRFA----RQLPLD-----KDFQLIRKKNRR*KKTGIKAQHIVNIDKVLVSFNMIRNHTIDYKGAKEVKIISTDHEKSNFTIILCAIRGGGKCTPIVNLKRKIFLPNEF 985 S K + R ++E WPELE ++ W+ ER K QVS + +K + L LG H + + R A + LPLD D+ L ++ GI + I N+D+V V+F+M T+D +G + V I++T EK+NFT++LC + G KC P+V KRK F Sbjct: 57 STKRANRGKREAWPELENELSTWVRSERNKGAQVSTIRIIREARKIARSLQLDDFLGTPHWCHSFMERKNLTVRRATSVGQSLPLDWEKKVADYHLFFQR----ATAGISQKDIGNMDEVPVAFDMPAARTVDIRGTQNVSIVTTGAEKANFTVVLCVLADGRKCQPMVIFKRKTLPKGPF 233
BLAST of SMED30019976 vs. TrEMBL
Match: A0A3P8P742 (Uncharacterized protein OS=Astatotilapia calliptera OX=8154 PE=4 SV=1) HSP 1 Score: 78.9518 bits (193), Expect = 1.868e-12 Identity = 67/222 (30.18%), Postives = 106/222 (47.75%), Query Frame = -2 Query: 317 SARYEKEFWPELEQDIKKWIIKERTKCRQVSM**FRSXXXXXXXXXXXXXXXXGDHDS*TDTNYRFARQLPLDKDFQLIRKKNRR*KKTG---IKAQHIVNIDKVLVSFNMIRNHTIDYKGAKEVKIISTDHEKSNFTIILCAIRGGGKCTPIVNLKRKIFLPNEFXXXXXXXXXXXVWLIKKSNVSGLKWSGVCEKMLSFKNIAKSFLIWDFVTPRVVEDI 973 S R K WPELE+ +++WII++RT R V R K T A+QLP D +L ++ K G I+ HI N+D+V ++F++ +HT++ KG V I +T HEKS+FT++L G K P+V KRK F +F +++K N W+ ++ L+ V + F + + S LI D + + D+ Sbjct: 57 SFRGHKARWPELEERLERWIIEQRTSGRSVLTVTIRLKAVSLSLFMKRCHFSIR-------TRTTVAQQLPADYKEKLAIFRSYCSKHIGDKNIQPSHITNMDEVPLTFDIPVSHTVEKKGTSTVAIRTTGHEKSSFTVVLGCHANGQKLPPMVIFKRKTFPKEKFPAGIIIKANEKGWMDEEMMKEWLREVYV-RRPGGFFHASPSLLICDSMRAHLTADV 270
BLAST of SMED30019976 vs. Planmine SMEST
Match: SMESG000052069.1 (SMESG000052069.1) HSP 1 Score: 63.929 bits (154), Expect = 7.932e-12 Identity = 55/177 (31.07%), Postives = 85/177 (48.02%), Query Frame = -2 Query: 497 KKASARYEKEFWPELEQDIKKWIIKERTKCRQVSM**FRSXXXXXXXXXXXXXXXXGDHDS*TDTNYR----------FARQLPLDKDFQLIRKKNRR*KKTGIKAQHIVNIDKVLVSFNMIRNHTIDYKGAKEVKIISTDHEKSNFTIILCAIRGGGKCTPIV-----NLKRKIFL 982 + +ARY PELE + +WI+ +R + QVS+ FR +LQ + +K TD + +QLP D +FQ+ + +N + I N D+V V +M +T++ KG EV+I +T HEK FT+ LC ++ G K P V N++R FL Sbjct: 9 RGGNARY-----PELETKLHEWILIQRERELQVSV--FRIRLQAQVIAKEMKIEGFAASSQLTDNFMKRKNICVRRTITKQQLPKDWEFQMAKFRN---TIADLLDNQIGNFDEVSVQCDMPLGYTVETKGTNEVRIKTTGHEKKRFTVNLCVLKDGTKLPPFVILGTRNIQRLSFL 175
BLAST of SMED30019976 vs. Planmine SMEST
Match: SMESG000055010.1 (SMESG000055010.1) HSP 1 Score: 38.5058 bits (88), Expect = 1.051e-7 Identity = 16/33 (48.48%), Postives = 22/33 (66.67%), Query Frame = -2 Query: 881 KASARYEKEFWPELEQDIKKWIIKERTKCRQVS 979 K + R+ KEFWPELE +K W++ ER +VS Sbjct: 5 KRARRFRKEFWPELESVLKDWVLSERLNGSRVS 37 HSP 2 Score: 36.1946 bits (82), Expect = 1.051e-7 Identity = 18/36 (50.00%), Postives = 23/36 (63.89%), Query Frame = -1 Query: 708 W*PRFMNRHQLSVCATTSVGQRLSID*EEKSAIKKD 815 W RFM RH+LS+ A TSVGQ L D +EK ++ Sbjct: 68 WCIRFMRRHRLSIRAVTSVGQNLPEDWKEKCVFDEE 103
BLAST of SMED30019976 vs. Planmine SMEST
Match: SMESG000040582.1 (SMESG000040582.1) HSP 1 Score: 50.8322 bits (120), Expect = 5.566e-7 Identity = 28/77 (36.36%), Postives = 41/77 (53.25%), Query Frame = -2 Query: 494 KTGIKAQHIVNIDKVLVSFNMIRNHTIDYKGAKEVKIISTDHEKSNFTIILCAIRGGGKCTPIVNLKRK----IFLP 712 K + I N D+V + +M +T++ KGA EV+I + HEK FT+ LC ++ G K P V L K F+P Sbjct: 148 KKDLPDNQIGNFDEVSIQCDMTLGYTVETKGANEVRIKTKGHEKKRFTVNLCVLKDGTKLPPFVILGTKKHTNTFIP 224 The following BLAST results are available for this feature:
BLAST of SMED30019976 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30019976 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30019976 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30019976 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 3
BLAST of SMED30019976 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30019976 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30019976 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30019976 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30019976 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30019976 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30019976 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30019976 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30019976 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30019976 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 3
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30019976 ID=SMED30019976|Name=SMED30019976|organism=Schmidtea mediterranea sexual|type=transcript|length=1061bpback to top protein sequence of SMED30019976-orf-1 >SMED30019976-orf-1 ID=SMED30019976-orf-1|Name=SMED30019976-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=113bp MIRNHTIDYKGAKEVKIISTDHEKSNFTIILCAIRGGGKCTPIVNLKRKIback to top Annotated Terms
The following terms have been associated with this transcript:
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