Transposon Ty3-I Gag-Pol polyprotein

Overview


Name	Transposon Ty3-I Gag-Pol polyprotein
Smed ID	SMED30019956
Length (bp)	2143

Neoblast Clusters

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of Transposon Ty3-I Gag-Pol polyprotein (SMED30019956) t-SNE clustered cells

Violin plots show distribution of expression levels for Transposon Ty3-I Gag-Pol polyprotein (SMED30019956) in cells (dots) of each of the 12 neoblast clusters.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30019956

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 2

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication	Specimen	Lifecycle	Evidence
Smed sexual biotype	SMED30019956	SMESG000081553.1	Contig35549	newmark_ests	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30019956	SMESG000081553.1	Contig35549	uc_Smed_v2	PMID:29674431 Fincher et al., 2018

Note: Hover over icons to view figure legend

Homology

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BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Zebrafish
Match: BX511224.1 (pep chromosome:GRCz11:2:18017000:18022765:1 gene:ENSDARG00000113243.1 transcript:ENSDART00000186877.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:BX511224.1)

HSP 1 Score: 140.584 bits (353), Expect = 9.346e-34
Identity = 96/296 (32.43%), Postives = 145/296 (48.99%), Query Frame = 2

Query:  491 EIIHRSNSPWASALYMVRKTNGSYQPCGYYRRLNYKTIPDAYPMPLIHDIVNRFEGCFIFFKL--------VPVAPENIPKTAITIPFALFECLCIPFGLRNATQTFQRLIDQVFVDL--PYMAVYVDDIIIGSDSCVNHIKHLSEVFRPLLNHNLGINQTKCLFAKDSVSFLGHIISGSGLKPLPEKVNTVKTFPN-----------------LKF*HNYAAL*VSSTFIINLLRRGIY*YKEALEDAVA-----------LASPAENAE*NLKADTSDVAIGA*LHQ--VIDGK 1258
            +II  S+SP  +  + V+K +GS +PC  YR LN  T+ + YP+PL+     R +G   F KL        V + P +  KTA   P   FE   +PFGL NA   FQ L++ V  D+   ++ VY+DDI+I S S   HI+H+  V + LL + L +   KC+F   SV FLGHI+S  G++  PEK+  V  +P                   +F HN++ L    T + +   +  + +  A E A +           L +P  + +  ++ D S+V +GA L Q    DGK
Sbjct:  551 KIIRPSSSPAGAGFFFVKKKDGSLRPCIDYRGLNSITVKNTYPLPLMSSAFERLQGANFFTKLDLRNAYHLVCIRPGDEWKTAFNTPRGHFEYCVLPFGLSNAPAVFQALVNDVLRDMIDQFIYVYLDDILIFSHSLQEHIQHVRRVLQRLLENGLYVKAEKCVFHAQSVQFLGHIVSVEGMRMDPEKIQAVVNWPTPDSRKALQRFLGFANFYRRFIHNFSQLAAPLTSLTS--SKTPFRWSSAAEAAFSKLKGCFVSAPILIAPDPSRQFVVEVDASEVGVGAILSQRSASDGK 844

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BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Zebrafish
Match: BX546500.1 (pep chromosome:GRCz11:23:12926092:12931693:-1 gene:ENSDARG00000086495.3 transcript:ENSDART00000122176.3 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:BX546500.1)

HSP 1 Score: 136.732 bits (343), Expect = 1.160e-32
Identity = 75/197 (38.07%), Postives = 109/197 (55.33%), Query Frame = 2

Query:  491 EIIHRSNSPWASALYMVRKTNGSYQPCGYYRRLNYKTIPDAYPMPLIHDIVNRFEGCFIFFKL--------VPVAPENIPKTAITIPFALFECLCIPFGLRNATQTFQRLIDQVFVDL--PYMAVYVDDIIIGSDSCVNHIKHLSEVFRPLLNHNLGINQTKCLFAKDSVSFLGHIISGSGLKPLPEKVNTVKTFPN 1051
            +II  S+SP  +  + V+K +GS +PC  YR LN  T+ + YP+PL+     R +G   F KL        V + P +  KTA   P   FE   +PFGL NA   FQ L++ V  D+   ++ VY+DDI+I S S   H++H+  V + LL + L +   KC+F   SV FLGHI+S  G++  PEK+  V  +P 
Sbjct:  525 KIIRPSSSPAGAGFFFVKKKDGSLRPCIDYRGLNSITVKNTYPLPLMSSAFERLQGANFFTKLDLRNAYHLVRIRPGDEWKTAFNTPRGHFEYCVLPFGLSNAPAVFQALVNDVLRDMIDQFIYVYLDDILIFSHSLQEHVQHVRRVLQRLLENGLYVKAEKCVFHAQSVQFLGHIVSVEGMRMDPEKIQAVVDWPT 721

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BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Zebrafish
Match: CR925755.2 (pep chromosome:GRCz11:17:42486740:42492668:-1 gene:ENSDARG00000116402.1 transcript:ENSDART00000183946.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CR925755.2)

HSP 1 Score: 135.961 bits (341), Expect = 2.721e-32
Identity = 74/197 (37.56%), Postives = 108/197 (54.82%), Query Frame = 2

Query:  491 EIIHRSNSPWASALYMVRKTNGSYQPCGYYRRLNYKTIPDAYPMPLIHDIVNRFEGCFIFFKL--------VPVAPENIPKTAITIPFALFECLCIPFGLRNATQTFQRLIDQVFVDL--PYMAVYVDDIIIGSDSCVNHIKHLSEVFRPLLNHNLGINQTKCLFAKDSVSFLGHIISGSGLKPLPEKVNTVKTFPN 1051
            +II  S+SP  +  + V+K +GS +PC  YR LN  T+ + YP+PL+     R +G   F KL        V + P +  KTA   P   FE   +PFGL NA   FQ L++ V  D+   ++ VY+DDI+I S S   H++H+  V + LL + L +   KC+F   SV FLGH +S  G++  PEK+  V  +P 
Sbjct:  524 KIIRPSSSPAGAGFFFVKKKDGSLRPCIDYRGLNSITVKNTYPLPLMSSAFERLQGANFFTKLDLRNAYHLVRIRPGDEWKTAFNTPRGHFEYCVLPFGLSNAPAVFQALVNDVLRDMIDQFIYVYLDDILIFSHSLQEHVQHVRRVLQRLLENGLYVKAEKCVFHAQSVQFLGHTVSVEGMRMDPEKIQAVVNWPT 720

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BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Zebrafish
Match: BX511082.1 (pep chromosome:GRCz11:9:14291932:14297132:1 gene:ENSDARG00000113678.1 transcript:ENSDART00000183119.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:BX511082.1)

HSP 1 Score: 135.191 bits (339), Expect = 4.787e-32
Identity = 75/197 (38.07%), Postives = 108/197 (54.82%), Query Frame = 2

Query:  491 EIIHRSNSPWASALYMVRKTNGSYQPCGYYRRLNYKTIPDAYPMPLIHDIVNRFEGCFIFFKL--------VPVAPENIPKTAITIPFALFECLCIPFGLRNATQTFQRLIDQVFVDL--PYMAVYVDDIIIGSDSCVNHIKHLSEVFRPLLNHNLGINQTKCLFAKDSVSFLGHIISGSGLKPLPEKVNTVKTFPN 1051
            +II  S+SP  +  + V+K +GS +PC  YR LN  T+ + YP+PL+     R +G   F KL        V +   +  KTA   P   FE   +PFGL NA   FQ L++ V  D+   ++ VY+DDI+I S S   H++H+  V + LL + L +   KC+F   SV FLGHI+S  G++  PEKV  V  +P 
Sbjct:  551 KIIRPSSSPAGAGFFFVKKKDGSLRPCIDYRGLNAITVKNTYPLPLMSSAFERLQGASFFTKLDLRNAYHLVRIREGDEWKTAFNTPRGHFEYCVLPFGLSNAPAVFQALVNDVLRDMLDQFIYVYLDDILIFSHSLQEHVQHVRRVLQRLLENGLYVKAEKCVFHAQSVPFLGHIVSVEGMRMDPEKVQAVVDWPT 747

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BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Zebrafish
Match: CR855320.1 (pep chromosome:GRCz11:1:7956030:7961696:1 gene:ENSDARG00000099359.2 transcript:ENSDART00000159655.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CR855320.1)

HSP 1 Score: 131.724 bits (330), Expect = 5.403e-31
Identity = 73/197 (37.06%), Postives = 107/197 (54.31%), Query Frame = 2

Query:  491 EIIHRSNSPWASALYMVRKTNGSYQPCGYYRRLNYKTIPDAYPMPLIHDIVNRFEGCFIFFKL--------VPVAPENIPKTAITIPFALFECLCIPFGLRNATQTFQRLIDQVFVDL--PYMAVYVDDIIIGSDSCVNHIKHLSEVFRPLLNHNLGINQTKCLFAKDSVSFLGHIISGSGLKPLPEKVNTVKTFPN 1051
            +II  S+SP  +  + V+K +GS +PC  YR LN  T+ + YP+PL+     R +G   F KL        V +   +  K+A   P   FE   +PFGL NA   FQ  ++ V  D+   ++ VY+DDI+I S S   H++H+  V + LL + L +   KC+F   SV FLGHI+S  GL+  PEK+  V  +P 
Sbjct:  553 KIIRPSSSPAGAGFFFVKKKDGSLRPCIDYRGLNNITVKNTYPLPLMSSAFERLQGANFFTKLDLRNAYHLVRIRAGDEWKSAFNTPRGHFEYCVLPFGLSNAPAVFQAFVNDVLRDMIDQFIYVYLDDILIFSHSLQEHVQHIRRVLQRLLENGLYVKAEKCVFHAQSVPFLGHIVSVEGLRMDPEKIKAVVNWPT 749

HSP 2 Score: 52.7582 bits (125), Expect = 1.763e-6
Identity = 43/146 (29.45%), Postives = 66/146 (45.21%), Query Frame = 2

Query: 1379 LQPSSGYRYILTMMDRK*K*PEAVPL-----ADFIANSVVRTFFSTWIARYGVSVHVIKDQGTQFESDIFRNLSKSYGFKLVRTTAYHSKTNGLIERYHRTLKNSLRTL--SNKYDWIRAFPIVLLG*RNTYSDTTKASPAQMLFG 1795
            L PS+G   ILT++DR  K    VPL     A   A +V+   F      +G+   V+ D+G QF S  +R   +  G  +  ++ +H ++NG  ER ++ L+ +LR L   N   W +    V     +     T  SP Q   G
Sbjct: 1084 LPPSNGNTVILTVVDRFSKAAHFVPLPKLPSARETAVAVINHVFRI----HGLPTDVVSDRGPQFISKFWREFCRLLGATVSLSSGFHPQSNGQTERANQDLERTLRCLVSQNPSSWSQQLSWVEYAHNSLPVSATGLSPFQCSLG 1225

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BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Xenopus
Match: anxa6 (annexin A6 [Source:Xenbase;Acc:XB-GENE-989741])

HSP 1 Score: 140.584 bits (353), Expect = 5.701e-34
Identity = 92/299 (30.77%), Postives = 144/299 (48.16%), Query Frame = 2

Query:  461 ESEFREIEKMEIIHRSNSPWASALYMVRKTNGSYQPCGYYRRLNYKTIPDAYPMPLIHDIVNRFEGCFIFFKL--------VPVAPENIPKTAITIPFALFECLCIPFGLRNATQTFQRLIDQVFVDLP-YMAVYVDDIIIGSDSCVNHIKHLSEVFRPLLNHNLGINQTKCLFAKDSVSFLGHIISGSGLKPLPEKVNTVKTFP-----------------NLKF*HNYAAL*VSSTFIINLLRRGIY*Y-----------KEALEDAVALASPAENAE*NLKADTSDVAIGA*LHQV 1246
            +S+  E+    +I  S+SPWAS + +V K +GS + C  YRRLN  T  DAYPMP + ++++R         L        +P+AP    K+A   PF L++   +PFG+RNA  TFQRL++++   +  +   Y+DDI + S +   H++HL  VF  + +  L +   KC  A   V +LGH + G  L+P P KV  +  +P                   KF  NY+ +    T + +  R     +           K+AL  +  LA+P  +    L+ D S+  +GA L QV
Sbjct:  354 KSQIDEMLAFGVITPSHSPWASPVVLVPKKDGSTRFCVDYRRLNDVTTTDAYPMPRVDELLDRLGNAKYLTTLDLSRGYWQIPLAPSAQEKSAFLTPFGLYQFTVMPFGMRNAPATFQRLVNRLLEGMQDFAQAYLDDIAVFSQTWEEHLQHLQRVFAQIQDAGLTLKPEKCHLAMAEVQYLGHRVGGGQLRPDPAKVEAICQWPIPKTQKQVLAFLGTSGYYRKFIPNYSTVAKPLTDLTSRQRSRTIVWTPECESAMNALKQALASSPVLAAPDFSRRFILQTDASNFGLGAVLSQV 652

HSP 2 Score: 81.2629 bits (199), Expect = 3.371e-15
Identity = 49/142 (34.51%), Postives = 78/142 (54.93%), Query Frame = 2

Query: 1418 MDRK*K*PEAVPL----ADFIANSVVRTFFSTWIARYGVSVHVIKDQGTQFESDIFRNLSKSYGFKLVRTTAYHSKTNGLIERYHRTLKNSLRTL--SNKYDWIRAFPIVLLG*RNTYSDTTKASPAQMLFGTSTTIPVNLL 1825
            MD   + PEAV L    A  +A+++++ F     +R G    ++ DQG QF S + + L +  G + + ++ YH +TNGL ER++ TLK  LRT   S + DW R  P +L   R    ++T  SP ++L+G     P++LL
Sbjct:    1 MDYATRYPEAVALRKIDAPTVADALIQIF-----SRVGFPSEILSDQGPQFTSQLLQCLWQRCGVRAIHSSPYHPQTNGLCERFNGTLKTMLRTFVESGEKDWERYLPHLLFAYREVPQESTGFSPFELLYGRRVRGPLDLL 137

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BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Xenopus
Match: ENSXETT00000006041.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:6:37317867:37322724:1 gene:ENSXETG00000003070.1 transcript:ENSXETT00000006041.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 129.413 bits (324), Expect = 3.953e-30
Identity = 74/204 (36.27%), Postives = 109/204 (53.43%), Query Frame = 2

Query:  470 FREIEKMEIIHRSNSPWASALYMVRKTNGSYQPCGYYRRLNYKTIPDAYPMPLIHDIVNRFEGCFIFFKL--------VPVAPENIPKTAITIPFALFECLCIPFGLRNATQTFQRLIDQVFVDLPYMA--VYVDDIIIGSDSCVNHIKHLSEVFRPLLNHNLGINQTKCLFAKDSVSFLGHIISGSGLKPLPEKVNTVKTFPN 1051
              E++   II  S SP+AS + +VRK NGS + C  YR LN +TIPD Y  P I D +N   G   F  L        +P+ PE+  KTA   P   FE   +P GL  A  TFQRL+++   D+  +   VY+DD+I+   +   H + L +VF  L    L ++  KC F + SV+++GH++S  G+   P K+  V ++P 
Sbjct:  346 LEELKAAGIIKESRSPYASPIVVVRKKNGSIRMCVDYRTLNQRTIPDQYTTPRIEDALNCLVGSKWFSVLDLRSGYYQLPMHPEDKEKTAFICPLGFFEFNRMPQGLCGAPATFQRLMERTVGDMHLLEVLVYLDDLIVFGRTLEEHEQRLLKVFDRLEKEGLKLSPEKCQFCQPSVNYVGHVVSAEGIATDPSKIEAVSSWPK 549

HSP 2 Score: 65.855 bits (159), Expect = 2.049e-10
Identity = 46/157 (29.30%), Postives = 72/157 (45.86%), Query Frame = 2

Query: 1367 ICI---SLQP-SSGYRYILTMMDRK*K*PEAVPLADFIANSVVRTFFSTWIARYGVSVHVIKDQGTQFESDIFRNLSKSYGFKLVRTTAYHSKTNGLIERYHRTLKNSLRTL--SNKYDWIRAFPIVLLG*RNTYSDTTKASPAQMLFGTSTTIPVN 1819
            +CI   S++P   G   +L + D   +  +A P  D  A +V +     +   YG+   +  DQG  FES +   L    G    RTT YH + +   ER++RTL + L TL    K  W R    V+    +T +  T  SP  ++FG    +P++
Sbjct:  978 VCIDFLSIEPDEGGTSNVLVVTDHYTRYAQAFPTKDQRAITVAKVLVERFFIHYGLPKRIHSDQGRDFESKLVHELMSMLGVLKSRTTPYHPQGDPQPERFNRTLLDMLGTLPKEKKTHWSRHIATVVHAYNSTKNGATGYSPYFLMFGREARLPID 1134

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BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Xenopus
Match: lin54 (lin-54 DREAM MuvB core complex component [Source:Xenbase;Acc:XB-GENE-5752559])

HSP 1 Score: 111.694 bits (278), Expect = 1.909e-27
Identity = 66/194 (34.02%), Postives = 99/194 (51.03%), Query Frame = 2

Query:  497 IHRSNSPWASALYMVRKTNGSYQPCGYYRRLNYKTIPDAYPMPLIHDIVNRFEGCFIFFKL--------VPVAPENIPKTAITIPFALFECLCIPFGLRNATQTFQRLIDQVFVDL--PYMAVYVDDIIIGSDSCVNHIKHLSEVFRPLLNHNLGINQTKCLFAKDSVSFLGHIISGSGLKPLPEKVNTVKTFP 1048
            I  S SP  +  + V K +G  +PC  YR LN  T+ + YP+PLI ++ ++ +G  IF KL        + +   +  KTA       +E L +PFGL NA   FQ  ++ +F DL    + VY+DDI+I S     H   + E    L  ++L     KC F    +SFLG+IIS  G +  P KV+ ++ +P
Sbjct:  549 IRPSTSPAGAGFFFVEKKDGGLRPCIDYRGLNKITVKNRYPLPLISELFDQLKGAKIFSKLDLRGAYNLIRIREGDEWKTAFNTRDGHYEYLVMPFGLCNAPAVFQEFVNDIFRDLLGKSVVVYLDDILIFSQDLETHRSQVKEALSRLRENSLFAKLDKCTFEVPKISFLGYIISSRGFEMDPAKVSAIQKWP 742

HSP 2 Score: 31.9574 bits (71), Expect = 1.909e-27
Identity = 12/27 (44.44%), Postives = 17/27 (62.96%), Query Frame = 1

Query: 1036 QNFPKPQILTQLRSFVGIINFYHQFIK 1116
            Q +P PQ    ++ F+G  N+Y QFIK
Sbjct:  739 QKWPLPQSTKAIQRFIGFANYYRQFIK 765

HSP 3 Score: 66.2402 bits (160), Expect = 1.774e-10
Identity = 46/144 (31.94%), Postives = 69/144 (47.92%), Query Frame = 2

Query: 1373 ISLQPSSGYRYILTMMDRK*K*PEAVPLADFIANSVVRTFFSTWIAR-YGVSVHVIKDQGTQFESDIFRNLSKSYGFKLVRTTAYHSKTNGLIERYHRTLKNSLRTLSN--KYDWIRAFPIVLLG*RNTYSDTTKASPAQMLFG 1795
            + L PS G   I  ++DR  K    VPL    +   +   F   I R +G  V ++ D+G+QF S  +R+L KS G  L  ++AYH +TNG  ER ++ L+  LR   +  + DW    P       N    +T  SP   ++G
Sbjct: 1076 VELPPSCGNTVIWVVIDRFSKMAHFVPLRKLPSAVELAQLFIQHIFRLHGFPVEIVSDRGSQFVSRFWRSLCKSLGVSLQFSSAYHPQTNGAAERVNQALEQFLRNHVSLCQDDWSDLLPWAEFAHNNASHSSTGRSPFLSVYG 1219

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BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Xenopus
Match: castor1 (cytosolic arginine sensor for mTORC1 subunit 1 [Source:Xenbase;Acc:XB-GENE-960689])

HSP 1 Score: 111.694 bits (278), Expect = 2.040e-27
Identity = 66/194 (34.02%), Postives = 99/194 (51.03%), Query Frame = 2

Query:  497 IHRSNSPWASALYMVRKTNGSYQPCGYYRRLNYKTIPDAYPMPLIHDIVNRFEGCFIFFKL--------VPVAPENIPKTAITIPFALFECLCIPFGLRNATQTFQRLIDQVFVDL--PYMAVYVDDIIIGSDSCVNHIKHLSEVFRPLLNHNLGINQTKCLFAKDSVSFLGHIISGSGLKPLPEKVNTVKTFP 1048
            I  S SP  +  + V K +G  +PC  YR LN  T+ + YP+PLI ++ ++ +G  IF KL        + +   +  KTA       +E L +PFGL NA   FQ  ++ +F DL    + VY+DDI+I S     H   + E    L  ++L     KC F    +SFLG+IIS  G +  P KV+ ++ +P
Sbjct:  549 IRPSTSPAGAGFFFVEKKDGGLRPCIDYRGLNKITVKNRYPLPLISELFDQLKGAKIFSKLDLRGAYNLIRIREGDEWKTAFNTRDGHYEYLVMPFGLCNAPAVFQEFVNDIFRDLLGKSVVVYLDDILIFSQDLETHRSQVKEALSRLRENSLFAKLDKCTFEVPKISFLGYIISSRGFEMDPAKVSAIQKWP 742

HSP 2 Score: 31.9574 bits (71), Expect = 2.040e-27
Identity = 12/27 (44.44%), Postives = 17/27 (62.96%), Query Frame = 1

Query: 1036 QNFPKPQILTQLRSFVGIINFYHQFIK 1116
            Q +P PQ    ++ F+G  N+Y QFIK
Sbjct:  739 QKWPLPQSTKAIQRFIGFANYYRQFIK 765

HSP 3 Score: 65.855 bits (159), Expect = 2.359e-10
Identity = 46/144 (31.94%), Postives = 69/144 (47.92%), Query Frame = 2

Query: 1373 ISLQPSSGYRYILTMMDRK*K*PEAVPLADFIANSVVRTFFSTWIAR-YGVSVHVIKDQGTQFESDIFRNLSKSYGFKLVRTTAYHSKTNGLIERYHRTLKNSLRTLSN--KYDWIRAFPIVLLG*RNTYSDTTKASPAQMLFG 1795
            + L PS G   I  ++DR  K    VPL    +   +   F   I R +G  V ++ D+G+QF S  +R+L KS G  L  ++AYH +TNG  ER ++ L+  LR   +  + DW    P       N    +T  SP   ++G
Sbjct: 1076 VELPPSCGNTVIWVVIDRFSKMAHFVPLRKLPSAVELAQLFIQHIFRLHGFPVEIVSDRGSQFVSRFWRSLCKSLGVSLQFSSAYHPQTNGAAERVNQALEQFLRNHVSLCQDDWSDLLPWAEFAHNNASHSSTGRSPFLSVYG 1219

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BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Xenopus
Match: ENSXETT00000020886.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:3:49613601:49618752:-1 gene:ENSXETG00000011904.1 transcript:ENSXETT00000020886.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 111.694 bits (278), Expect = 2.040e-27
Identity = 66/194 (34.02%), Postives = 99/194 (51.03%), Query Frame = 2

Query:  497 IHRSNSPWASALYMVRKTNGSYQPCGYYRRLNYKTIPDAYPMPLIHDIVNRFEGCFIFFKL--------VPVAPENIPKTAITIPFALFECLCIPFGLRNATQTFQRLIDQVFVDL--PYMAVYVDDIIIGSDSCVNHIKHLSEVFRPLLNHNLGINQTKCLFAKDSVSFLGHIISGSGLKPLPEKVNTVKTFP 1048
            I  S SP  +  + V K +G  +PC  YR LN  T+ + YP+PLI ++ ++ +G  IF KL        + +   +  KTA       +E L +PFGL NA   FQ  ++ +F DL    + VY+DDI+I S     H   + E    L  ++L     KC F    +SFLG+IIS  G +  P KV+ ++ +P
Sbjct:  549 IRPSTSPAGAGFFFVEKKDGGLRPCIDYRGLNKITVKNRYPLPLISELFDQLKGAKIFSKLDLRGAYNLIRIREGDEWKTAFNTRDGHYEYLVMPFGLCNAPAVFQEFVNDIFRDLLGKSVVVYLDDILIFSQDLETHRSQVKEALSRLRENSLFAKLDKCTFEVPKISFLGYIISSRGFEMDPAKVSAIQKWP 742

HSP 2 Score: 31.9574 bits (71), Expect = 2.040e-27
Identity = 12/27 (44.44%), Postives = 17/27 (62.96%), Query Frame = 1

Query: 1036 QNFPKPQILTQLRSFVGIINFYHQFIK 1116
            Q +P PQ    ++ F+G  N+Y QFIK
Sbjct:  739 QKWPLPQSTKAIQRFIGFANYYRQFIK 765

HSP 3 Score: 65.855 bits (159), Expect = 2.359e-10
Identity = 46/144 (31.94%), Postives = 69/144 (47.92%), Query Frame = 2

Query: 1373 ISLQPSSGYRYILTMMDRK*K*PEAVPLADFIANSVVRTFFSTWIAR-YGVSVHVIKDQGTQFESDIFRNLSKSYGFKLVRTTAYHSKTNGLIERYHRTLKNSLRTLSN--KYDWIRAFPIVLLG*RNTYSDTTKASPAQMLFG 1795
            + L PS G   I  ++DR  K    VPL    +   +   F   I R +G  V ++ D+G+QF S  +R+L KS G  L  ++AYH +TNG  ER ++ L+  LR   +  + DW    P       N    +T  SP   ++G
Sbjct: 1076 VELPPSCGNTVIWVVIDRFSKMAHFVPLRKLPSAVELAQLFIQHIFRLHGFPVEIVSDRGSQFVSRFWRSLCKSLGVSLQFSSAYHPQTNGAAERVNQALEQFLRNHVSLCQDDWSDLLPWAEFAHNNASHSSTGRSPFLSVYG 1219

[Close]

BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. UniProt/SwissProt
Match: sp|Q99315|YG31B_YEAST (Transposon Ty3-G Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY3B-G PE=1 SV=3)

HSP 1 Score: 129.798 bits (325), Expect = 1.834e-29
Identity = 72/192 (37.50%), Postives = 104/192 (54.17%), Query Frame = 2

Query:  506 SNSPWASALYMVRKTNGSYQPCGYYRRLNYKTIPDAYPMPLIHDIVNRFEGCFIFFKL--------VPVAPENIPKTAITIPFALFECLCIPFGLRNATQTFQRLIDQVFVDLPYMAVYVDDIIIGSDSCVNHIKHLSEVFRPLLNHNLGINQTKCLFAKDSVSFLGHIISGSGLKPLPEKVNTVKTFPNLK 1057
            S SP +S + +V K +G+++ C  YR LN  TI D +P+P I ++++R     IF  L        +P+ P++  KTA   P   +E   +PFGL NA  TF R +   F DL ++ VY+DDI+I S+S   H KHL  V   L N NL + + KC FA +   FLG+ I    + PL  K   ++ FP  K
Sbjct:  628 SKSPCSSPVVLVPKKDGTFRLCVDYRTLNKATISDPFPLPRIDNLLSRIGNAQIFTTLDLHSGYHQIPMEPKDRYKTAFVTPSGKYEYTVMPFGLVNAPSTFARYMADTFRDLRFVNVYLDDILIFSESPEEHWKHLDTVLERLKNENLIVKKKKCKFASEETEFLGYSIGIQKIAPLQHKCAAIRDFPTPK 819

[Close]

BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. UniProt/SwissProt
Match: sp|Q7LHG5|YI31B_YEAST (Transposon Ty3-I Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY3B-I PE=1 SV=2)

HSP 1 Score: 129.413 bits (324), Expect = 1.953e-29
Identity = 72/192 (37.50%), Postives = 104/192 (54.17%), Query Frame = 2

Query:  506 SNSPWASALYMVRKTNGSYQPCGYYRRLNYKTIPDAYPMPLIHDIVNRFEGCFIFFKL--------VPVAPENIPKTAITIPFALFECLCIPFGLRNATQTFQRLIDQVFVDLPYMAVYVDDIIIGSDSCVNHIKHLSEVFRPLLNHNLGINQTKCLFAKDSVSFLGHIISGSGLKPLPEKVNTVKTFPNLK 1057
            S SP +S + +V K +G+++ C  YR LN  TI D +P+P I ++++R     IF  L        +P+ P++  KTA   P   +E   +PFGL NA  TF R +   F DL ++ VY+DDI+I S+S   H KHL  V   L N NL + + KC FA +   FLG+ I    + PL  K   ++ FP  K
Sbjct:  654 SKSPCSSPVVLVPKKDGTFRLCVDYRTLNKATISDPFPLPRIDNLLSRIGNAQIFTTLDLHSGYHQIPMEPKDRYKTAFVTPSGKYEYTVMPFGLVNAPSTFARYMADTFRDLRFVNVYLDDILIFSESPEEHWKHLDTVLERLKNENLIVKKKKCKFASEETEFLGYSIGIQKIAPLQHKCAAIRDFPTPK 845

[Close]

BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. UniProt/SwissProt
Match: sp|P04323|POL3_DROME (Retrovirus-related Pol polyprotein from transposon 17.6 OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 126.716 bits (317), Expect = 1.200e-28
Identity = 71/211 (33.65%), Postives = 115/211 (54.50%), Query Frame = 2

Query:  461 ESEFREIEKMEIIHRSNSPWASALYMVRKTNGSYQPCGY-----YRRLNYKTIPDAYPMPLIHDIVNRFEGCFIF--------FKLVPVAPENIPKTAITIPFALFECLCIPFGLRNATQTFQRLIDQVFVDL--PYMAVYVDDIIIGSDSCVNHIKHLSEVFRPLLNHNLGINQTKCLFAKDSVSFLGHIISGSGLKPLPEKVNTVKTFP 1048
            ES+ +++    II  SNSP+ S +++V K   +     +     YR+LN  T+ D +P+P + +I+ +   C  F        F  + + PE++ KTA +     +E L +PFGL+NA  TFQR ++ +   L   +  VY+DDII+ S S   H++ L  VF  L   NL +   KC F K   +FLGH+++  G+KP PEK+  ++ +P
Sbjct:  224 ESQIQDMLNQGIIRTSNSPYNSPIWVVPKKQDASGKQKFRIVIDYRKLNEITVGDRHPIPNMDEILGKLGRCNYFTTIDLAKGFHQIEMDPESVSKTAFSTKHGHYEYLRMPFGLKNAPATFQRCMNDILRPLLNKHCLVYLDDIIVFSTSLDEHLQSLGLVFEKLAKANLKLQLDKCEFLKQETTFLGHVLTPDGIKPNPEKIEAIQKYP 434

[Close]

BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. UniProt/SwissProt
Match: sp|P20825|POL2_DROME (Retrovirus-related Pol polyprotein from transposon 297 OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 126.716 bits (317), Expect = 1.201e-28
Identity = 77/211 (36.49%), Postives = 116/211 (54.98%), Query Frame = 2

Query:  461 ESEFREIEKMEIIHRSNSPWASALYMV-RKTNGS----YQPCGYYRRLNYKTIPDAYPMPLIHDIVNRFEGCFIF--------FKLVPVAPENIPKTAITIPFALFECLCIPFGLRNATQTFQRLIDQVFVDL--PYMAVYVDDIIIGSDSCVNHIKHLSEVFRPLLNHNLGINQTKCLFAKDSVSFLGHIISGSGLKPLPEKVNTVKTFP 1048
            E++ +E+    +I  SNSP+ S  ++V +K + S    Y+    YR+LN  TIPD YP+P + +I+ +   C  F        F  + +  E+I KTA +     +E L +PFGLRNA  TFQR ++ +   L   +  VY+DDIII S S   H+  +  VF  L + NL +   KC F K   +FLGHI++  G+KP P KV  + ++P
Sbjct:  223 ENQVQEMLNQGLIRESNSPYNSPTWVVPKKPDASGANKYRVVIDYRKLNEITIPDRYPIPNMDEILGKLGKCQYFTTIDLAKGFHQIEMDEESISKTAFSTKSGHYEYLRMPFGLRNAPATFQRCMNNILRPLLNKHCLVYLDDIIIFSTSLTEHLNSIQLVFTKLADANLKLQLDKCEFLKKEANFLGHIVTPDGIKPNPIKVKAIVSYP 433

[Close]

BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. UniProt/SwissProt
Match: sp|P10401|POLY_DROME (Retrovirus-related Pol polyprotein from transposon gypsy OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 118.627 bits (296), Expect = 4.604e-26
Identity = 75/216 (34.72%), Postives = 116/216 (53.70%), Query Frame = 2

Query:  449 SRIAESEFREIEKMEIIHRSNSPWASALYMVRK--TNGSYQPCGY----YRRLNYKTIPDAYPMPLIHDIVNRFEGCFIFFKL--------VPVAPENIPKTAITIPFALFECLCIPFGLRNATQTFQRLIDQVFVDL--PYMAVYVDDIIIGSDSCVNHIKHLSEVFRPLLNHNLGINQTKCLFAKDSVSFLGHIISGSGLKPLPEKVNTVKTFP 1048
            S    +E +++ K  II  S SP+ S  ++V K  T+    P       +R+LN KTIPD YPMP I  I+        F  L        + +A  +  KT+ ++    +E   +PFGLRNA+  FQR +D V  +       VYVDD+II S++  +H++H+  V + L++ N+ ++Q K  F K+SV +LG I+S  G K  PEKV  ++ +P
Sbjct:  194 SDFVNNEVKQLLKDGIIRPSRSPYNSPTWVVDKKGTDAFGNPNKRLVIDFRKLNEKTIPDRYPMPSIPMILANLGKAKFFTTLDLKSGYHQIYLAEHDREKTSFSVNGGKYEFCRLPFGLRNASSIFQRALDDVLREQIGKICYVYVDDVIIFSENESDHVRHIDTVLKCLIDANMRVSQEKTRFFKESVEYLGFIVSKDGTKSDPEKVKAIQEYP 409

[Close]

BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. TrEMBL
Match: A0A4Y2W1J5 (Retrovirus-related Pol polyprotein from transposon 17.6 OS=Araneus ventricosus OX=182803 GN=pol_1001 PE=4 SV=1)

HSP 1 Score: 303.523 bits (776), Expect = 1.699e-89
Identity = 188/510 (36.86%), Postives = 266/510 (52.16%), Query Frame = 2

Query:  494 IIHRSNSPWASALYMVRKTNGSYQPCGYYRRLNYKTIPDAYPMPLIHDIVNRFEGCFIFFKL--------VPVAPENIPKTAITIPFALFECLCIPFGLRNATQTFQRLIDQVFVDLPYMAVYVDDIIIGSDSCVNHIKHLSEVFRPLLNHNLGINQTKCLFAKDSVSFLGHIISGSGLKPLPEKVNTVKTFPNLKF*HNYAAL*VSSTFIINLLRR-----------------------------------GIY*YKEALEDAVALASPAENAE*NLKADTSDVAIGA*LHQVIDGKRSHWRFFHESSLNANGNIRHST----------ESSWESLNQ*NIFSICISLQ----------PSSGYRYILTMMDRK*K*PEAVPLADFIANSVVRTFFSTWIARYGVSVHVIKDQGTQFESDIFRNLSKSYGFKLVRTTAYHSKTNGLIERYHRTLKNSLRTLSNKYDWIRAFPIVLLG*RNTYSDTTKASPAQMLFGTSTTIPVNLLHNP 1834
            I+  S+SPWAS L+MV+K+NG ++PCG YRRLN  TIPD YP+P I D    F    IF  L        +PV P +IPKTAIT PF LFE + +PFGLRNA QTFQR I QV  +L +   Y DD++I S++   H +HL +VF  L  H L +N  KC+  K SV FLG +I+  G+KPLPEKV  +  FP  +   N A L      ++N  RR                                        K  + +A  L   A N + ++  D SD+AIGA LH           F+  S   +    ++ST          E+ W  L +  I S+ + +           PS    Y+LT +DR  + PEAVP++D  A +V R F S WI+R+G+   +  DQG QFES++F  LSK  G + +RTT YH  +NG++ER+HR+LK SLR  ++   W  + P+VLLG R    +  + + A++++G++  +P +    P
Sbjct:    5 IVRPSSSPWASPLHMVKKSNGEWRPCGDYRRLNAITIPDRYPVPHIQDCTQIFFNKTIFSTLDLMRAYHQIPVNPADIPKTAITTPFGLFEYVFMPFGLRNAGQTFQRYIHQVLSNLGFCVPYFDDVLIASNNSEEHKQHLKQVFERLSQHGLKLNPLKCVLGKPSVKFLGCLITSEGVKPLPEKVQAISQFPKPQ---NIAEL-KRFLAMLNFYRRFLPNAADTQASLHEFLKNSKKNDKRPVSWTDVTLAAFEKCKADIINAATLTFHAPNQQLSIMVDASDLAIGAVLHTTTSLGHKPLAFY--SRKLSPSERKYSTYPCHLTLDWAETVWPFLCK-RISSLDLPMSGLPKIQGPLPPSDNCEYLLTCIDRFTRWPEAVPISDISAETVARAFISQWISRFGLPSIITTDQGRQFESNLFSFLSKLLGVQKIRTTPYHPSSNGIVERFHRSLKQSLRCHAST-KWTESIPVVLLGLRTALKEDLQCTSAELVYGSTLKLPADFFETP 506

[Close]

BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. TrEMBL
Match: A0A085ND72 (Uncharacterized protein OS=Trichuris suis OX=68888 GN=M514_20454 PE=4 SV=1)

HSP 1 Score: 279.641 bits (714), Expect = 2.565e-78
Identity = 206/629 (32.75%), Postives = 295/629 (46.90%), Query Frame = 2

Query:  113 HQFQMILFRLFLYQPIIGSNFLAHNGFCR*LLIQITNH--VECISQSSDNQNSSTFKHT--MTTRVYHQ*KC*NNFME---LITEPIYQTFRR*LTSNINNCCSAYHSNQS*PISVY*TSR-------IAESEFREIEKMEIIHRSNSPWASALYMVRKTNGSYQPCGYYRRLNYKTIPDAYPMPLIHDIVNRFEGCFIFFKL--------VPVAPENIPKTAITIPFALFECLCIPFGLRNATQTFQRLIDQVFVDLPYMAVYVDDIIIGSDSCVNHIKHLSEVFRPLLNHNLGINQTKCLFAKDSVSFLGHIISGSGLKPLPEKVNTVKTFPN----------LKF*HNYAAL*VSSTFIINLLRRGIY-----------------*YKEALEDAVALASPAENAE*NLKADTSDVAIGA*LHQV---IDGKRSHWRFFHESSLNANGNIRHS-----TESSWESLNQ*NIFSI---CISLQPSSGYR----------------------------------YILTMMDRK*K*PEAVPLADFIANSVVRTFFSTWIARYGVSVHVIKDQGTQFESDIFRNLSKSYGFKLVRTTAYHSKTNGLIERYHRTLKNSLRTLSNKYDWIRA 1717
            H F+       + QP++G++FL  N     +L+ + N   V+  S +S N   +   H   + +   H   C    +     IT P + T       N+ +  + Y      P+    T R       IA++EFR++E+M I+ RSNSPW+S L+MV K +G ++PCG YRRLN  T PD YP+P I D      G  +F K+        +PV  E+I KTA+T PF LFE L +PFGL+NA Q FQRL+D V   L +  VY+DDI+I S     H+ HL  +F+ L +H L IN  KC F   S+ FLGH I   G  PLP+K++ ++ F            L   + Y     S+  I+  L + +                    KEAL  A  L  P  NA   +  D SD A+GA L Q    I       R F      ++ +IR +     T+  W  + +  I S    CI  Q S   R                                  Y+ T++DR  + PEA+PL D  A S  R     WIAR+G+   +  D+G+QF S ++  +++  G KL RTTAYH + NGL+E +HR LK++LRT     DWI++
Sbjct:  313 HHFKWTFTVADVSQPLLGADFLRANS----ILVDVENQRLVDTKSFTSINLRRAHAVHAPHLGSIASHT-SCYAKLLAKFPAITTPQFAT------GNLKHGVAHYIQTLGPPVHAR-TRRLPPDKLQIAKNEFRKMEEMGIVRRSNSPWSSPLHMVPKASGGWRPCGDYRRLNKATTPDRYPVPHIQDFSANLHGTKLFSKIDLVRGYHQIPVHSEDIAKTAVTTPFGLFEFLRMPFGLKNAAQAFQRLMDTVCCGLDFAFVYLDDILIASRDHREHLDHLRMLFQRLQDHGLIINPAKCKFGLASIDFLGHRIDPHGAVPLPDKMDAIRKFAKPTTVRALQEFLGMANFYHRFVPSAAKIMQPLYKALVDNPKELVWNETTAAAFANTKEALAKATMLVHPQPNARTAVTVDASDAAVGAVLEQFPRPIIPTSWRRRIFDAIHGLSHPSIRTTQKLMATKFVWHGMRK-QIASWAKCCIPCQTSKVQRHTKAPLQRFELADRRFDHIHVDIVGPLPPCRGNRYLFTIVDRFTRWPEAIPLPDTSATSCARAIIGHWIARFGIPNRMPSDRGSQFTSTLWSGMAQLLGIKLHRTTAYHPQANGLVETFHRHLKSALRTRLTGPDWIKS 928

[Close]

BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. TrEMBL
Match: X1WR82 (Uncharacterized protein OS=Acyrthosiphon pisum OX=7029 PE=4 SV=1)

HSP 1 Score: 253.832 bits (647), Expect = 1.076e-67
Identity = 178/555 (32.07%), Postives = 261/555 (47.03%), Query Frame = 2

Query:  452 RIAESEFREIEKMEIIHRSNSPWASALYMVRKTNGSYQPCGYYRRLNYKTIPDAYPMPLIHDIVNRFEGCFIFFKL--------VPVAPENIPKTAITIPFALFECLCIPFGLRNATQTFQRLIDQVFVDLPYMAVYVDDIIIGSDSCVNHIKHLSEVFRPLLNHNLGINQTKCLFAKDSVSFLGHIISGSGLKPLPEKVNTVKTFPNLKF*HNYAAL*VSSTFIINL---------LRRGIY*YKE------------------------ALEDAVALASPAENAE*NLKADTSDVAIGA*LHQVIDGKRSHWRFFHESSLNANGNIRHSTES-------SWESLNQ------*NIFS----ICISLQPSS---GYRYILT-----------------MMDRK*K*PEAVPLADFIANSVVRTFFSTWIARYGVSVHVIKDQGTQFESDIFRNLSKSYGFKLVRTTAYHSKTNGLIERYHRTLKNSLRTLSNKYDWIRAFPIVLLG*RNTYSDTTKASPAQMLFGTSTTIPVNLLHNPEPPTSNELDFTRFNLR 1882
            RIA  EF  + +  I   S S WAS L +V K +G+++PCG YRRLN  T  D YP+P +HD      G  +F KL        +P+A ++IPKTA+T PF LFE   + FG RNA QTFQR+I+ +   L +   Y+DD++I S +   H +H+  V     N+ + IN  KC+F  DS++FLGH I+    +P  E++  +  +P       +A +     F+  L         L+  +Y                            AL D   LA P  N    L  D S+ AIGA L Q+ D       F+ +    A   +  S           W S +Q       +I S      + L+P S   G  Y+ T                 M+ +     EA P+ +  A++V  T  + WI+R+GV   V  DQG QFES++F  L+K+ G + +R++ YH + NG++ER HRTLK +L T  +   W    PIVLL  RNT       +PA+M++G S  +P ++ + P PP +        NLR
Sbjct:  885 RIARQEFEYMRQKGICRPSASAWASPLLLVPKKDGTFRPCGDYRRLNEVTRADRYPLPHLHDFTTNLAGNTVFTKLDLVRAYHQIPIAKQDIPKTAVTTPFGLFEFPVMCFGRRNAAQTFQRVINDMLRGLNFSFAYIDDVLIVSRNHEEHEQHVRTVLGRCQNYGIAINPVKCVFTVDSLTFLGHNINKDACRPTSERITAIHEWPLPTTTEKFAEISGLRKFLSTLYSVIRNAAELQAPLYDLAAKIKKPDGPLSWTDETRNVFESCRIALADTAELAHPLPNVPLRLSTDASNTAIGAVLEQLSDKGWQPLGFYSKKLSKAEKKLDVSATQVVLPDLKQW-SADQAGDPELKDILSGSTKSALKLEPWSTAEGTIYLDTSTKQARMNEQANQVLGQMLHKMPTHQEAWPMDNMSAHAVATTLTTQWISRFGVPEVVTTDQGRQFESELFTALTKNLGIQHLRSSPYHPQANGMVERLHRTLKTAL-TAHDTVSWSLRLPIVLLALRNTVKPDIGHTPAEMVYGMSLRLPGDMFY-PAPPEAKTAPELARNLR 1436

HSP 2 Score: 127.872 bits (320), Expect = 1.458e-29
Identity = 68/168 (40.48%), Postives = 101/168 (60.12%), Query Frame = 2

Query: 1379 LQPSSGYRYILTMMDRK*K*PEAVPLADFIANSVVRTFFSTWIARYGVSVHVIKDQGTQFESDIFRNLSKSYGFKLVRTTAYHSKTNGLIERYHRTLKNSLRTLSNKYDWIRAFPIVLLG*RNTYSDTTKASPAQMLFGTSTTIPVNLLHNPEPPTSNELDFTRFNLR 1882
            L PS+G +Y+LT +D   + PEA P+ +  A++V  T  + WI+R+GV   V  DQG QFES++F  L+K+ G + +R++ YH + NG++ER HRTLK +L T  +   W    PIVLL  RNT       +PA+M++G S  +P ++ H P PP +        NLR
Sbjct:  307 LPPSNGCKYLLTCVDSFTRWPEAWPMDNMSAHAVATTLTTQWISRFGVPDVVTTDQGRQFESELFTALTKNLGIQHLRSSPYHPQANGMVERLHRTLKTAL-TAHDTVSWSLRLPIVLLALRNTVKPDIGHAPAEMVYGRSLRLPGDMFH-PAPPDAKTAPELARNLR 472

HSP 3 Score: 33.113 bits (74), Expect = 1.458e-29
Identity = 22/55 (40.00%), Postives = 26/55 (47.27%), Query Frame = 1

Query: 1936 RVRSVQDTSLSPRYHRP*KLKEIKNSTAIIETHQGATTVNFARLKPAFCRRELPV 2100
            RV +VQ   L PRY  P  + E       I+   G   V+  RLKPAF  RE P 
Sbjct:  507 RVDAVQ-PPLQPRYEGPYAVLERHEKNFKIQRDNGTVLVSIDRLKPAFMLREDPA 560

[Close]

BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. TrEMBL
Match: A0A0C2NLD3 (Transposon Ty3-I Gag-Pol polyprotein OS=Thelohanellus kitauei OX=669202 GN=RF11_07028 PE=4 SV=1)

HSP 1 Score: 225.328 bits (573), Expect = 7.101e-60
Identity = 129/310 (41.61%), Postives = 172/310 (55.48%), Query Frame = 2

Query:  455 IAESEFREIEKMEIIHRSNSPWASALYMVRKTNGSYQPCGYYRRLNYKTIPDAYPMPLIHDIVNRFEGCFIFFKL--------VPVAPENIPKTAITIPFALFECLCIPFGLRNATQTFQRLIDQVFVDLPYMAVYVDDIIIGSDSCVNHIKHLSEVFRPLLNHNLGINQTKCLFAKDSVSFLGHIISGSGLKPLPEKVNTVKTFPN-------------LKF*HN------------YAAL*VSSTFII--NLLRRGIY*YKEALEDAVALASPAENAE*NLKADTSDVAIGA*LHQVIDGKRSHWRFF 1279
            +A+ EFR +E++ II RSNSPW+S L+MV K +G Y+PCG YR LN  TIPD YP+P I D  N   GC IF K+        +PV P +IPKTA+  PF LFE L +PFGL+NA QTFQR +D V  DLP++ VY+DDI++ S+S   H++HL  +F  L +++L IN  KC F   ++ FLG+ +S  G+ PLPEKV  + +FP              +   H             Y AL   S  ++  + +       KEAL  A  L  P  NA   L  D S +A+GA L Q  DG      FF
Sbjct:  418 LAKKEFRRMEELGIIRRSNSPWSSPLHMVPKPSGDYRPCGDYRNLNDATIPDRYPIPHIQDFTNHLAGCKIFSKIDLIRGYNQIPVHPPDIPKTAVITPFGLFEFLRMPFGLKNAAQTFQRFMDSVVQDLPFLYVYLDDILVFSNSVKEHLEHLKRLFERLHSNHLMINLEKCKFGTSTIDFLGYHVSQEGIIPLPEKVEAIYSFPKPISKKELQQFNGMINHYHRFIPAVANLMKPLYCALKNKSKLVVWSDEMDVAFQKVKEALAKATLLVYPQPNAPLALTTDASTIAVGAVLEQYNDGYWQPLSFF 727

HSP 2 Score: 37.3502 bits (85), Expect = 7.101e-60
Identity = 18/35 (51.43%), Postives = 23/35 (65.71%), Query Frame = 1

Query: 1264 PLAFFSRKLTQC*RKYSTFDRELLGITESIKHFQY 1368
            PL+FFSR+L      YS FDRELL +  S  HF++
Sbjct:  723 PLSFFSRQLRTPELNYSAFDRELLAMYLSTHHFRH 757

[Close]

BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. TrEMBL
Match: A0A418XSS2 (RNA-directed DNA polymerase OS=Alcanivorax profundi OX=2338368 GN=D4A39_16565 PE=4 SV=1)

HSP 1 Score: 215.312 bits (547), Expect = 8.111e-60
Identity = 101/207 (48.79%), Postives = 135/207 (65.22%), Query Frame = 2

Query:  455 IAESEFREIEKMEIIHRSNSPWASALYMVRKTNGSYQPCGYYRRLNYKTIPDAYPMPLIHDIVNRFEGCFIFFKL--------VPVAPENIPKTAITIPFALFECLCIPFGLRNATQTFQRLIDQVFVDLPYMAVYVDDIIIGSDSCVNHIKHLSEVFRPLLNHNLGINQTKCLFAKDSVSFLGHIISGSGLKPLPEKVNTVKTFPN 1051
            IA  EF  +E + I+ RSNSPWAS L+MV KT+G ++PCG +RRLN  T PD YP+P I D      G  IF K+        VPV P+++PKTA+  PF LFE L +PFGL+ A QTFQRL+D V  D+P++ VY+DDI++ S S  +H+ HL ++F  L  H L IN  KC F + S++FLGH ++  G  PLP KV+ V +FP 
Sbjct:   69 IAREEFATMEHLGIVRRSNSPWASPLHMVTKTDGGWRPCGDFRRLNNATTPDRYPVPHIQDFSAHLSGAIIFSKVDLVRGYHQVPVHPQDVPKTAVITPFGLFEFLRMPFGLKGAAQTFQRLMDSVLRDMPFLFVYLDDILVASASADDHLIHLRQLFGRLSEHGLIINPAKCEFGRSSITFLGHHVTPQGAVPLPAKVDAVASFPR 275

[Close]

BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Cavefish
Match: ENSAMXT00000041345.1 (pep primary_assembly:Astyanax_mexicanus-2.0:25:32334536:32339002:1 gene:ENSAMXG00000029230.1 transcript:ENSAMXT00000041345.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 160.614 bits (405), Expect = 2.477e-40
Identity = 101/305 (33.11%), Postives = 151/305 (49.51%), Query Frame = 2

Query:  449 SRIAESEFREIEKMEIIHRSNSPWASALYMVRKTNGSYQPCGYYRRLNYKTIPDAYPMPLIHDIVNRFEGCFIFFKL--------VPVAPENIPKTAITIPFALFECLCIPFGLRNATQTFQRLIDQVFVDLPYMA--VYVDDIIIGSDSCVNHIKHLSEVFRPLLNHNLGINQTKCLFAKDSVSFLGHIISGSGLKPLPEKVNTVKTFPNLK-------------F*HNYAAL*VSSTFIINLLRRGIY*Y-------------KEALEDAVALASPAENAE*NLKADTSDVAIGA*LHQVIDG 1255
             ++ +    ++++  I+  S SPWAS + +V K  G  + C  YRR+N KT  DAYPMP + +I+    G  IF  L        V + P++IPKTA      L+E   +PFG++NA  TFQRL+D V V+L   +  VY++DI++ S +   H+ HL EVFR L    L +N  KC   + S+ FLGH+ISG G+   P KV  ++ FP  +             + H +         I+N L++    +             K+AL  A  L  P  +    ++ D SD  +GA L Q  DG
Sbjct:  641 QQLIKEAIEDMQRRGIVRPSTSPWASPVVLVPKKEGGVRFCVDYRRMNSKTHLDAYPMPQVQEILESLHGAAIFSTLDLKSGYWQVGLEPDSIPKTAFITCQGLYEFTVLPFGIKNAAATFQRLMDSVLVNLKGKSCFVYINDIVVYSSTIEQHLGHLEEVFRCLHQAGLTLNLRKCNLLQRSLIFLGHVISGEGICTEPGKVEAIQAFPEPRSIKELQRFLGMAGWYHRFITHFSERAAILNALKKKNAPWIWTQECQKAFEDIKQALITAPVLTPPNFSEPFQIQTDASDQGLGAVLSQGTDG 945

HSP 2 Score: 61.6178 bits (148), Expect = 2.887e-9
Identity = 44/165 (26.67%), Postives = 80/165 (48.48%), Query Frame = 2

Query: 1403 YILTMMDRK*K*PEAVPLADFIANSVVRTFFSTWIARYGVSVHVIKDQGTQFESDIFRNLSKSYGFKLVRTTAYHSKTNGLIERYHRTLKNSLRT-LSNKYD-WIRAFPIVLLG*RNTYSDTTKASPAQMLFGTSTTIPVNLLHNPEPPTSNELDFTRFNLRAQD 1891
            ++L ++D   K  E  PL +   + +V+        R+G   +++ D+G QF S +     + +G     TTAYH +TN L ER +RTLK  + + + +K+  W +  P       + + ++T  +PA++  G     P+  L + +PP  +  D  +   R QD
Sbjct: 1220 HLLVIVDYCSKWVEMFPLREAKTSQIVQILIKDIFTRWGTPAYLVSDRGAQFTSRLLHATCRQWGVVQKLTTAYHPQTN-LTERVNRTLKTMIASYVKDKHRLWDQWIPEFRFAINSAWQESTGFTPAEVALGRKLKGPLERLLS-QPPNPDH-DAYKVVQRQQD 1381

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BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Cavefish
Match: ENSAMXT00000029916.1 (pep primary_assembly:Astyanax_mexicanus-2.0:10:19390924:19392876:1 gene:ENSAMXG00000040471.1 transcript:ENSAMXT00000029916.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 128.257 bits (321), Expect = 6.292e-36
Identity = 84/301 (27.91%), Postives = 140/301 (46.51%), Query Frame = 2

Query:  452 RIAESEFREIEKMEIIHRSNSPWASALYMVRKTNGSYQPCGYYRRLNYKTIPDAYPMPLIHDIVNRFEGCFIFFKL--------VPVAPENIPKTAITIPFALFECLCIPFGLRNATQTFQRLIDQVFVDLPYMAVYVDDIIIGSDSCVNHIKHLSEVFRPLLNHNLGINQTKCLFAKDSVSFLGHIISGSGLKPLPEKVNTVKTFPN-----------------LKF*HNYAAL*VSSTFIINLLRRGIY*Y------------KEALEDAVALASPAENAE*NLKADTSDVAIGA*LHQ 1243
            +I   E   ++++ +I    S W+S + +V K +G+ + C  YR++N  T  D +P+P + D ++R        KL        VP++      +A   P  L+ C  +PFG++NA  TFQRL++Q+   L  + +Y+DD ++ S+S  +H+KHL E+F+ L    L +N  KC   K  V++LGH +    + P   KV  +   P                   +F  N++A+   +  + +LLR+ +  Y            K  L     L +P  N    L  D  DV +GA L Q
Sbjct:  244 KIVREELAYMQEIGVIEPGQSDWSSPVVLVPKPDGTVRFCVDYRKVNLVTKSDTFPIPRLDDCIDRIGKAKCVSKLDLLKGYWQVPLSDRAQKVSAFVTPEGLYCCKVLPFGMKNAPATFQRLMNQITAGLANVVIYLDDAVVHSESWQDHVKHLRELFQRLHEAQLVVNLPKCELGKGQVTYLGHQVGQGKVIPRQAKVQAILDLPCPRTRRELMRVLGMCGFYRRFVPNFSAV---TEPLTHLLRKDVKFYWTEECAKAFDQIKAVLACRPVLVAPDFNVPFKLAVDACDVGVGAVLLQ 541

HSP 2 Score: 43.5134 bits (101), Expect = 6.292e-36
Identity = 20/52 (38.46%), Postives = 36/52 (69.23%), Query Frame = 1

Query: 1264 PLAFFSRKLTQC*RKYSTFDRELLGITESIKHFQYMYITSAKLRLSLYFDYD 1419
            P+A+FS+KL +  R YST ++E L +  +I+HF+ +Y++S    L +Y D++
Sbjct:  550 PVAYFSKKLNKHQRAYSTIEKEALALVLAIRHFE-VYVSSVVGDLVVYTDHN 600

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BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Cavefish
Match: ENSAMXT00000054230.1 (pep primary_assembly:Astyanax_mexicanus-2.0:14:19483082:19487098:1 gene:ENSAMXG00000038338.1 transcript:ENSAMXT00000054230.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 143.665 bits (361), Expect = 7.784e-35
Identity = 77/207 (37.20%), Postives = 117/207 (56.52%), Query Frame = 2

Query:  455 IAESEFREIEKMEIIHRSNSPWASALYMVRKTNGSYQPCGYYRRLNYKTIPDAYPMPLIHDIVNRFEGCFIFFKL--------VPVAPENIPKTAITIPFALFECLCIPFGLRNATQTFQRLIDQVFVDLPYMA--VYVDDIIIGSDSCVNHIKHLSEVFRPLLNHNLGINQTKCLFAKDSVSFLGHIISGSGLKPLPEKVNTVKTF 1045
            + ++   ++ + +++  S+SP+AS + +V+K +GS + C  YR LN KT  DA+P+P I + ++   G   F  L        VPVA ++ PKTA   PF LFE   +PFGL NA  TFQRL+ ++F D  Y +  +Y+DDII+ S S   H++ L  V   L    L    +KC+F K  V +LGH+IS  G+   P K+  V  +
Sbjct:  314 LVKAHIDQLLEAQVVRESSSPYASPIVLVKKKDGSLRMCVDYRLLNSKTRKDAFPLPRIEESLDALSGARWFSTLDLASGYNQVPVAEQDKPKTAFCTPFGLFEWNRMPFGLCNAPSTFQRLMQRMFGDQQYQSLLLYLDDIIVFSSSVSQHLQRLEVVLGRLQKEGLKAKLSKCVFFKQEVGYLGHVISNKGVSTDPAKIEAVAQW 520

HSP 2 Score: 93.2041 bits (230), Expect = 4.374e-19
Identity = 60/165 (36.36%), Postives = 81/165 (49.09%), Query Frame = 2

Query: 1379 LQPS-SGYRYILTMMDRK*K*PEAVPLADFIANSVVRTFFSTWIARYGVSVHVIKDQGTQFESDIFRNLSKSYGFKLVRTTAYHSKTNGLIERYHRTLKNSLRTL--SNKYDWIRAFPIVLLG*RNTYSDTTKASPAQMLFGTSTTIPVN-LLHNPEPPTSNELD 1861
            L+PS +G   +L M D   K   AVP  D  A++V +     W  ++GV   +  DQG  FES + +     YG +  RTT YH   NG  ER++RTL N LRTL  S K DW    P V+     T    T  SP  ++FG    +PV+ LL   + P   E+ 
Sbjct:  928 LEPSRNGLENVLVMTDVFSKYTIAVPTRDQRASTVAQVLLREWFYKFGVPGRIHSDQGRSFESALIQQFCHLYGVERSRTTPYHPAGNGQCERFNRTLHNLLRTLPTSRKQDWASNLPQVIFCYNTTPHQGTGESPYYLMFGQEPRLPVDFLLGQVQEPVPGEVQ 1092

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BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Cavefish
Match: ENSAMXT00000045469.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000976.1:426503:430633:1 gene:ENSAMXG00000030479.1 transcript:ENSAMXT00000045469.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 142.895 bits (359), Expect = 1.129e-34
Identity = 78/204 (38.24%), Postives = 121/204 (59.31%), Query Frame = 2

Query:  455 IAESEFREIEKMEIIHRSNSPWASALYMVRKTNGSYQPCGYYRRLNYKTIPDAYPMPLIHDIVNRFEGCFIFFKL--------VPVAPENIPKTAITIPFALFECLCIPFGLRNATQTFQRLIDQVFVDLPY--MAVYVDDIIIGSDSCVNHIKHLSEVFRPLLNHNLGINQTKCLFAKDSVSFLGHIISGSGLKPLPEKVNTV 1036
            + ++  +E+ + +++  S+SP+AS + +V+K +GS + C  YR+LN KT  DA+P+P I + ++   G   F  L        VPV  ++  KTA   PF LFE   +PFGL NA  TFQRL++++F D  +  + +Y+DDI+I S     H++ L  V   L  HNL +   KC F +  VS+LGH+IS SG++  PEK+  V
Sbjct:  371 LVKAHIQELVEHQVVRPSSSPYASPIVVVQKKDGSIRLCVDYRQLNAKTRKDAFPLPRIEESLDALSGSKWFSTLDLASGYNQVPVLEKDKEKTAFCTPFGLFEFNRMPFGLCNAPSTFQRLMERIFGDQSFHTLLLYLDDIVIFSSDLQQHLQRLDMVLSRLQQHNLKLKLEKCHFFQTRVSYLGHVISESGVETDPEKIRAV 574

HSP 2 Score: 97.0561 bits (240), Expect = 2.754e-20
Identity = 52/148 (35.14%), Postives = 81/148 (54.73%), Query Frame = 2

Query: 1388 SSGYRYILTMMDRK*K*PEAVPLADFIANSVVRTFFSTWIARYGVSVHVIKDQGTQFESDIFRNLSKSYGFKLVRTTAYHSKTNGLIERYHRTLKNSLRTL--SNKYDWIRAFPIVLLG*RNTYSDTTKASPAQMLFGTSTTIPVNLL 1825
            S+G   +L + D   K  +A  +AD  A++VVR     W   YGV   +  DQG  FE ++ ++L ++YG +  RTT YH + NG  ER++RTL + LRTL    K  W +  P +L     T   +T+ SP ++LFG    +P++ +
Sbjct:  996 SNGQENVLVLTDVFSKFTQAYAVADQKASTVVRVLVEKWFYVYGVPKRIHSDQGRSFEGELLKHLCETYGIEKSRTTPYHPEGNGQCERFNRTLHDLLRTLPPEKKRKWPQLLPHLLFAYNTTIHQSTQYSPYELLFGQKPQLPIDRM 1143

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BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Cavefish
Match: ENSAMXT00000041754.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02001938.1:55959:59870:1 gene:ENSAMXG00000043385.1 transcript:ENSAMXT00000041754.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 142.124 bits (357), Expect = 1.901e-34
Identity = 77/194 (39.69%), Postives = 114/194 (58.76%), Query Frame = 2

Query:  494 IIHRSNSPWASALYMVRKTNGSYQPCGYYRRLNYKTIPDAYPMPLIHDIVNRFEGCFIFFKL--------VPVAPENIPKTAITIPFALFECLCIPFGLRNATQTFQRLIDQVFVDLPYMAV--YVDDIIIGSDSCVNHIKHLSEVFRPLLNHNLGINQTKCLFAKDSVSFLGHIISGSGLKPLPEKVNTVKTF 1045
            +I  S+SP++S + +V K +GS + C  YR+LN KT  D+YP+P I + ++   G   F  L        VPV  ++  KTA   PF LFE   +PFGL NA  TFQRL++++F D  Y +V  Y+DD+I+ S +   H++ L EVF  L   NL +  +KC F +  VS+LGH++S  G+   P K+  VK +
Sbjct:  335 VIRESSSPFSSPIVLVTKKDGSLRLCVDYRQLNAKTRRDSYPLPRIEESLDALCGAKWFSTLDLASGYNQVPVEEKDKSKTAFCTPFGLFEFNRMPFGLCNAPGTFQRLMERIFGDCRYQSVLLYLDDVIVFSQTVEEHLERLEEVFSRLQKQNLKVKLSKCQFFQHQVSYLGHVVSAEGVTTDPAKIEVVKEW 528

HSP 2 Score: 84.7297 bits (208), Expect = 1.931e-16
Identity = 52/152 (34.21%), Postives = 76/152 (50.00%), Query Frame = 2

Query: 1379 LQPSS-GYRYILTMMDRK*K*PEAVPLADFIANSVVRTFFSTWIARYGVSVHVIKDQGTQFESDIFRNLSKSYGFKLVRTTAYHSKTNGLIERYHRTLKNSLRTL--SNKYDWIRAFPIVLLG*RNTYSDTTKASPAQMLFGTSTTIPVNLL 1825
            L+P+S G   +L + D   K  +A+   D  A++V       W  R+G  V +  DQG  FES + + L K Y  +  RTT+Y  + NG  ER++RTL + LRTL    K  W R  P +      T   TT  +P  ++FG    +PV+ L
Sbjct:  936 LEPASDGKENVLILTDIFTKYTQAIATKDQRASTVAWALVQHWFHRFGPPVRIHSDQGRNFESLLIKQLCKVYSIQKSRTTSYRPQGNGQCERFNRTLHDLLRTLPVEEKRHWPRHLPQLTFAYNTTPHQTTGQTPHFLMFGYHPRLPVDFL 1087

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BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Medaka
Match: ENSORLT00000040051.1 (pep primary_assembly:ASM223467v1:1:37354481:37359289:-1 gene:ENSORLG00000026212.1 transcript:ENSORLT00000040051.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 154.451 bits (389), Expect = 2.485e-38
Identity = 105/307 (34.20%), Postives = 152/307 (49.51%), Query Frame = 2

Query:  452 RIAESEFREIEKMEIIHRSNSPWASALYMVRKTNGSYQPCGYYRRLNYKTIPDAYPMPLIHDIVNRFEGCFIFFKL--------VPVAPENIPKTAITIPFALFECLCIPFGLRNATQTFQRLIDQVFVDLP--YMAVYVDDIIIGSDSCVNHIKHLSEVFRPLLNHNLGINQTKCLFAKDSVSFLGHIISGSGLKPLPEKVNTVKTFPN-------------LKF*HNYAAL*VSSTFIINLLR-------------RGIY*YKEALEDAVALASPAENAE*NLKADTSDVAIGA*LHQVI-DGKR 1261
            +I      ++ K  II  S S W + + +V K +GS + C  YRRLN KT  DAYPMPLIHD++    G   F  L        V +A ++  KTA+  P  L++   +PFGL+NA  TFQRL+++V  +L      VY+DD+II S S   H+K L  VF  L + NL +N  KC F +  + FLGHI+SG+G++  P+K   V T+P              + + H +          +N LR             R     K+AL  A  L  P  +    +  D SD+ +GA L Q+  +G+R
Sbjct:  746 KIITEHIDQMLKDGIIEPSQSAWGAPVVLVPKPDGSMRFCVDYRRLNAKTTADAYPMPLIHDLLESMHGAEFFSTLDLKSGYWQVAMAEDSKAKTAVITPVGLYQFKSMPFGLKNAGATFQRLMEKVLGELRGRLCCVYIDDVIIFSPSAEQHLKDLQAVFGKLHSANLVLNLKKCTFFQRQLRFLGHIVSGAGVQVDPDKSRAVMTYPEPTNVPSLQRFLGLVGWYHKFIPQFSDLAAPLNHLRKKGVEWRWTEECQRSFNQLKQALAKAPILVQPDLSLPFEVHTDASDLGLGAVLAQMTSEGER 1052

HSP 2 Score: 65.0846 bits (157), Expect = 2.420e-10
Identity = 52/173 (30.06%), Postives = 80/173 (46.24%), Query Frame = 2

Query: 1322 TESSWESLNQ*NIFSICISLQPSSGYRYILTMMDRK*K*PEAVPLADFIANSVVRTFFSTWIARYGVSVHVIKDQGTQFESDIFRNLSKSYGFKLVRTTAYHSKTNGLIERYHRTLKNSLRTL----SNKYD-WIRAFPIVLLG*RNTYSDTTKASPAQMLFGTSTTIPVNLL 1825
             E++WE L    I  +    +   G  Y++ ++D   K  E  PL D  A+ +V         RYGV   ++ D+G QF S    NL K++G     TT+YH + N L ER +RT+K  + +        +D WIR F   +        +TT  SPA+++ G     P+  L
Sbjct: 1303 AEAAWEKLG---IDLLGPLPRSKKGNVYLVVLVDYFTKWVEVFPLKDSKAHRIVTFLRDDIFTRYGVPRELVSDRGPQFTSHEMLNLCKTWGVIQRFTTSYHPQAN-LTERSNRTIKTMIASYVGSEHKNWDLWIREFRFAI---NAAQHETTGRSPAELMLGRGLKGPLERL 1468

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BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Medaka
Match: ENSORLT00000045600.1 (pep primary_assembly:ASM223467v1:4:26167161:26171153:-1 gene:ENSORLG00000023514.1 transcript:ENSORLT00000045600.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 140.969 bits (354), Expect = 4.973e-34
Identity = 76/207 (36.71%), Postives = 120/207 (57.97%), Query Frame = 2

Query:  455 IAESEFREIEKMEIIHRSNSPWASALYMVRKTNGSYQPCGYYRRLNYKTIPDAYPMPLIHDIVNRFEGCFIFFKL--------VPVAPENIPKTAITIPFALFECLCIPFGLRNATQTFQRLIDQVFVDLPYMAV--YVDDIIIGSDSCVNHIKHLSEVFRPLLNHNLGINQTKCLFAKDSVSFLGHIISGSGLKPLPEKVNTVKTF 1045
            +A S  +++ + ++I  S+SP+AS + +V+K +G  + C  YR+LN +T  DA+P+P I + ++   G   F  L        V VA ++  KTA   PF LFE   +PFGL NA  TFQRL++++F D  + +V  Y+DD+II S S   H++ L +VF  L    L +  +KC F +  V +LGH++S  G+   PEK   V+ +
Sbjct:  355 LAHSHIQQLLQSQVIRESSSPYASPIVLVQKKDGGLRMCVDYRQLNARTRKDAFPLPRIDESLDALAGAQWFTTLDLASGYSQVEVAEKDKAKTAFCTPFGLFEFNRMPFGLCNAPSTFQRLMERLFGDCRFQSVLLYLDDVIIFSSSVEQHLQRLEQVFSRLEAQGLKVKLSKCHFFQKQVKYLGHVVSAEGVSTDPEKAAVVRDW 561

HSP 2 Score: 79.7221 bits (195), Expect = 7.230e-15
Identity = 48/148 (32.43%), Postives = 67/148 (45.27%), Query Frame = 2

Query: 1388 SSGYRYILTMMDRK*K*PEAVPLADFIANSVVRTFFSTWIARYGVSVHVIKDQGTQFESDIFRNLSKSYGFKLVRTTAYHSKTNGLIERYHRTLKNSLRTL--SNKYDWIRAFPIVLLG*RNTYSDTTKASPAQMLFGTSTTIPVNLL 1825
            + G   +L M D   K  + VP  D  A++V       W   +G    +  DQG  FES++   L K Y     RTT YH + NG  ER++ TL + LRTL    K  W R    V      T   +T  +P  ++FG    +PV+ L
Sbjct:  964 TDGRENVLVMTDVFSKYTQTVPTKDQRASTVAEALVKHWFQLFGPPARIHSDQGWNFESNLVHQLCKFYQIDKSRTTPYHPQGNGQCERFNLTLHDLLRTLPPEQKRRWPRHLSQVTFAYNTTVHQSTGMTPYFLMFGREPRLPVDFL 1111

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BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Medaka
Match: ENSORLT00000045141.1 (pep primary_assembly:ASM223467v1:23:22495254:22500491:-1 gene:ENSORLG00000029628.1 transcript:ENSORLT00000045141.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 140.198 bits (352), Expect = 1.039e-33
Identity = 79/202 (39.11%), Postives = 112/202 (55.45%), Query Frame = 2

Query:  476 EIEKMEIIHRSNSPWASALYMVRKTNGSYQPCGYYRRLNYKTIPDAYPMPLIHDIVNRFEGCFIFFKL--------VPVAPENIPKTAITIPFALFECLCIPFGLRNATQTFQRLIDQVFVDLPY--MAVYVDDIIIGSDSCVNHIKHLSEVFRPLLNHNLGINQTKCLFAKDSVSFLGHIISGSGLKPLPEKVNTVKTFPN 1051
            E+    I+  S SPWAS   +V K +GS + C  YRRLN  T  DAYP+P + + ++      +F  L        V +  E+  KTA+T PF LFE   +PFGL NA  TFQRL+  V  DL +  + VY+DDI++ S    +H + L  VF+ L  H L +  +KC   +  V FLGH+IS  G+K   EKV+ + ++P 
Sbjct:  741 ELVSQGILKESTSPWASPAVIVIKKDGSVRFCCDYRRLNQVTCKDAYPLPRVEEALDALGDAHLFSTLDLTAGYFQVAMNEEDQGKTAVTTPFGLFEWTRMPFGLCNAPATFQRLMGLVLGDLSFDILLVYLDDILVYSRDFESHCERLGLVFQRLRQHGLKLKPSKCFLLRPEVKFLGHLISAQGIKVDMEKVSALTSWPT 942

HSP 2 Score: 87.8113 bits (216), Expect = 2.151e-17
Identity = 47/147 (31.97%), Postives = 72/147 (48.98%), Query Frame = 2

Query: 1391 SGYRYILTMMDRK*K*PEAVPLADFIANSVVRTFFSTWIARYGVSVHVIKDQGTQFESDIFRNLSKSYGFKLVRTTAYHSKTNGLIERYHRTLKNSLRTL--SNKYDWIRAFPIVLLG*RNTYSDTTKASPAQMLFGTSTTIPVNLL 1825
             GY  +L + D   +   AVP  +  A++  +     W A YG    +  DQG  FE+ + + L K YG    RT+ YH + N   ER++RT+ + LRTL    K DW    P + +   N    +T  SP  ++FG    +P++LL
Sbjct: 1362 GGYENVLVLTDMFTRFTMAVPTKNQTADTTAKALVKHWFAYYGCPARLHSDQGRSFEASVIKELCKIYGIAKSRTSPYHPQGNAQCERFNRTMHDMLRTLPPEKKRDWKAYLPELSMAYNNRVHSSTGYSPFYLMFGRDARMPLDLL 1508

[Close]

BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Medaka
Match: ENSORLT00000040501.1 (pep primary_assembly:ASM223467v1:1:1251188:1255180:-1 gene:ENSORLG00000023024.1 transcript:ENSORLT00000040501.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 139.813 bits (351), Expect = 1.192e-33
Identity = 76/207 (36.71%), Postives = 119/207 (57.49%), Query Frame = 2

Query:  455 IAESEFREIEKMEIIHRSNSPWASALYMVRKTNGSYQPCGYYRRLNYKTIPDAYPMPLIHDIVNRFEGCFIFFKL--------VPVAPENIPKTAITIPFALFECLCIPFGLRNATQTFQRLIDQVFVDLPYMAV--YVDDIIIGSDSCVNHIKHLSEVFRPLLNHNLGINQTKCLFAKDSVSFLGHIISGSGLKPLPEKVNTVKTF 1045
            +A S  +++ + ++I  S+SP+AS + +V+K +G  + C  YR+LN +T  DA+P P I + ++   G   F  L        V VA ++  KTA   PF LFE   +PFGL NA  TFQRL++++F D  + +V  Y+DD+II S S   H++ L +VF  L    L +  +KC F +  V +LGH++S  G+   PEK   V+ +
Sbjct:  355 LARSHIQQLLQSQVIRESSSPYASPIVLVQKKDGGLRMCVDYRQLNARTRKDAFPSPRIDESLDALAGAQWFTTLDLASGYSQVEVAEKDKAKTAFCTPFGLFEFNRMPFGLCNAPSTFQRLMERLFGDCRFQSVLLYLDDVIIFSSSVEQHLQRLEQVFSRLDAQGLKVKLSKCHFFQKQVKYLGHVVSAEGVSTDPEKAAVVRDW 561

HSP 2 Score: 83.1889 bits (204), Expect = 6.413e-16
Identity = 49/148 (33.11%), Postives = 68/148 (45.95%), Query Frame = 2

Query: 1388 SSGYRYILTMMDRK*K*PEAVPLADFIANSVVRTFFSTWIARYGVSVHVIKDQGTQFESDIFRNLSKSYGFKLVRTTAYHSKTNGLIERYHRTLKNSLRTL--SNKYDWIRAFPIVLLG*RNTYSDTTKASPAQMLFGTSTTIPVNLL 1825
            + G   +L M D   K  + VP  D  A++V       W   +G    +  DQG  FES++   L K Y     RTT YH + NG  ER++RTL + LRTL    K  W R    V      T   +T  +P  ++FG    +PV+ L
Sbjct:  964 TDGRENVLVMTDVFSKYTQTVPTKDQRASTVAEALVKHWFQLFGPPARIHSDQGRNFESNLVHRLCKFYQIDKSRTTPYHPQGNGQCERFNRTLHDLLRTLPPEQKRRWPRHLSQVTFAYNTTVHQSTGMTPYFLMFGREPRLPVDFL 1111

[Close]

BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Medaka
Match: ENSORLT00000045829.1 (pep primary_assembly:ASM223467v1:10:30089555:30096892:1 gene:ENSORLG00000028614.1 transcript:ENSORLT00000045829.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 129.413 bits (324), Expect = 2.920e-32
Identity = 76/180 (42.22%), Postives = 104/180 (57.78%), Query Frame = 2

Query:  494 IIHRSNSPWASALYMVRKTNGSYQPCGYYRRLNYKTIPDAYPMPLIHDIVNRFEGCFIFFKL--------VPVAPENIPKTAITIPFALFECLCIPFGLRNATQTFQRLIDQVFVDLPYMA--VYVDDIIIGSDSCVNHIKHLSEVFRPLLNHNLGINQTKCLFAKDSVSFLGHIISGSG 1003
            II  S+S WAS + +V K  GS + C  YR LN KT  DAYPMPL+H+I+   +G   F  L        V +   +  KTA+T    LF+   +PFGLRNA  TFQRL+++V  +L      VY+DDII+ S S   H++ L  VF  L + +L +N  KC F K  ++FLGH++S  G
Sbjct:  171 IIEPSSSAWASPVVLVPKKCGSLRFCVDYRGLNAKTHHDAYPMPLVHEILESMQGAKYFSSLDLQSGYWQVAMDEGSKYKTAMTTHRGLFQFRVMPFGLRNAGATFQRLMERVLGNLNGRVCFVYIDDIIVFSKSQEQHLRDLDAVFHKLHHAHLTLNVKKCHFFKTELTFLGHVVSARG 350

[Close]

BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Planmine SMEST
Match: SMESG000011773.1 (SMESG000011773.1)

HSP 1 Score: 160.614 bits (405), Expect = 1.130e-42
Identity = 80/160 (50.00%), Postives = 102/160 (63.75%), Query Frame = 2

Query:  458 AESEFREIEKMEIIHRSNSPWASALYMVRKTNGSYQPCGYYRRLNYKTIPDAYPMPLIHDIVNRFEGCFIFFKL--------VPVAPENIPKTAITIPFALFECLCIPFGLRNATQTFQRLIDQVFVDLPYMAVYVDDIIIGSDSCVNHIKHLSEVFRPL 913
            AE EF ++EK +II RS+SPWA+ + ++ K +GS + CG YR +N  TIPD+YPMPLI DI+ RF GC IF  L        + + PE+IPKTA+  PF LFE   +P GLRNA+QTFQR ID V   L +   YVDDII+ S     H K L E +  L
Sbjct:  238 AEKEFLQLEKQDIIRRSSSPWAAPIQIITKKDGSLRICGDYRSVNKITIPDSYPMPLISDILQRFSGCSIFSSLDLAKAYHQIQIEPESIPKTAVITPFCLFEYTFMPLGLRNASQTFQRHIDTVLQGLQHTVAYVDDIIVASPYIEEHKKDLCETYLTL 397

[Close]

BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Planmine SMEST
Match: SMESG000037879.1 (SMESG000037879.1)

HSP 1 Score: 149.828 bits (377), Expect = 1.261e-39
Identity = 78/205 (38.05%), Postives = 116/205 (56.59%), Query Frame = 2

Query:  458 AESEFREIEKMEIIHRSNSPWASALYMVRKTNGSYQPCGYYRRLNYKTIPDAYPMPLIHDIVNRFEGCFIFFKL--------VPVAPENIPKTAITIPFALFECLCIPFGLRNATQTFQRLIDQVFVDLPYMAVYVDDIIIGSDSCVNHIKHLSEVFRPLLNHNLGINQTKCLFAKDSVSFLGHIISGSGLKPLPEKVNTVKTFP 1048
             +SEF  +   +   RS SP +S L +  K +G+++PC   R LN  T+ D+Y MPL+ +I++R      F  +        +PV   +I KTA+  P  +FE + +PFGLRN+ QTF+R I QV +DLP+   Y+DDI+I S     H  HL+E    L  + L IN    +F K  V F GH+IS  G +P P++++T+  FP
Sbjct:  101 VQSEFSNLLNNDTTRRSKSPLSSPLNLFTKKDGTFRPCADCRNLNAITVHDSYSMPLVSNILHRLSKAKTFLTIDLVKAYHQIPVRESDIEKTAVLTPIGMFEYVFMPFGLRNSVQTFKRYIHQVLMDLPFAVPYMDDILIRSPEMDTHKIHLTETLNELQQNCLKINVNNWIFFKQEVRFHGHLISRKGTRP-PDRLDTISNFP 304

[Close]

BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Planmine SMEST
Match: SMESG000080881.1 (SMESG000080881.1)

HSP 1 Score: 127.872 bits (320), Expect = 8.584e-32
Identity = 76/206 (36.89%), Postives = 123/206 (59.71%), Query Frame = 2

Query:  458 AESEFREIEKMEIIHRSNSPWASALYMVRKTNGSYQPCGYYRRLNYKTIPDAYPMPLIHDIVNRFEGCFIFFKLV---------PVAPENIPKTAITIPFALFECLCIPFGLRNATQTFQRLIDQVFVDLPYMAVYVDDIIIGSDSCVNHIKHLSEVFRPLLNHNLGINQTKCLFAKDSVSFLGHIISGSGLKPLPEKVNTVKTFP 1048
            ++ + + +++ +II  S+SP++S++ +V+K +G  + C  YR+LN  T  D YP+P+  ++ ++  G   FF ++          V  E+  KTA +    L+E   +PFGL  A  TFQR ++ V  D+P+  VY+DDIII S++   H+K +  VFR LL  NL I  +KC + K  V ++GHIIS +GL+P PE V  +K +P
Sbjct: 3399 SDRQIKAMKEFKIIRDSSSPFSSSIAVVKKKDGDLRFCVDYRKLNQVTRKDTYPIPVPQEMFDQM-GEVQFFTVIDLNKGYNQFKVKEEDRRKTAFSDGKGLYEFNVLPFGLTGAPATFQRGMNFVLRDIPHALVYIDDIIIFSNTFKEHLKDVETVFRRLLKANLKIKPSKCEWGKSEVVYVGHIISRNGLQPNPENVKKIKEYP 3603

HSP 2 Score: 29.261 bits (64), Expect = 8.584e-32
Identity = 10/27 (37.04%), Postives = 20/27 (74.07%), Query Frame = 1

Query: 1036 QNFPKPQILTQLRSFVGIINFYHQFIK 1116
            + +P P+ + Q+R F+G+ ++Y +FIK
Sbjct: 3600 KEYPIPENVDQVRQFLGLASYYRKFIK 3626

HSP 3 Score: 64.6994 bits (156), Expect = 2.595e-10
Identity = 33/95 (34.74%), Postives = 57/95 (60.00%), Query Frame = 2

Query: 1400 RYILTMMDRK*K*PEAVPLADFIANSVVRTFFSTWIARYGVSVHVIKDQGTQFESDIFRNLSKSYGFKLVRTTAYHSKTNGLIERYHRTLKNSLR 1684
            +YI+   D   K PEA  + +  A +V + F    I RYG    ++ DQGT F+S++F ++++ +G   + T+  H +T+GL+ER+ RTL N ++
Sbjct: 3927 KYIIVFCDYLTKWPEAYAMQNQKAETVAKIFVENIIFRYGAPKKLLTDQGTNFQSELFISITELFGILKLSTSPCHPQTDGLVERFIRTLINMIK 4021

[Close]

BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Planmine SMEST
Match: SMESG000038549.1 (SMESG000038549.1)

HSP 1 Score: 131.724 bits (330), Expect = 4.202e-31
Identity = 84/263 (31.94%), Postives = 139/263 (52.85%), Query Frame = 2

Query:  455 IAESEFREIEKMEIIHRSNSPWASALYMVRKTNGSYQPCGYYRRLNYKTIPDAYPMPLIHDIVNRFEGCFIFFKL--------VPVAPENIPKTAITIPFALFECLCIPFGLRNATQTFQRLIDQVFVDLPYMAVYVDDIIIGSDSCVNHIKHLSEVFRPLLNHNLGINQTKCLFAKDSVSFLGHIISGSGLKPLPEKVNTVKTFPNLKF*HNYAAL*VSSTFIINLLRRGIY*YKEALEDAVALASPAENAE*NLKADTSDV 1219
            +AE E +++   ++I  S SPW   + MV+K +G+ + C YYR+LN  T  D YP+P I++++   +G   F  L        + +  E+  KTA TI   L++   +PFGL NA  +FQR ++ V   +    VY+DD++I S +   H++ + +VF  L    L +  +KC +AK+ V+FLGHI+S  G +P P  +  +K  P  K          + T I   L    Y Y++ +++   +A P ++    +K DTS V
Sbjct: 2103 VAEQEVQKMLDAKVIEPSCSPWQFPVVMVKKKDGTQRFCIYYRKLNAVTKRDTYPLPDINEMLLTLQGAAYFTSLDLKSGYWQIKMKEEDKEKTAFTIGRDLYQFKKMPFGLTNAPASFQRCMNFVTHGIKQCMVYIDDLLIYSSTFEQHLEDIRKVFIRLRQWKLKLKPSKCDWAKEKVTFLGHIVSAKGKEPDPRNIEKIKNCPAPK----------TVTQIQEFLGLCGY-YRKFIKNYAGIAKPIQDL---VKKDTSFV 2351

HSP 2 Score: 92.8189 bits (229), Expect = 6.212e-19
Identity = 53/173 (30.64%), Postives = 88/173 (50.87%), Query Frame = 2

Query: 1391 SGYRYILTMMDRK*K*PEAVPLADFIANSVVRTFFSTWIARYGVSVHVIKDQGTQFESDIFRNLSKSYGFKLVRTTAYHSKTNGLIERYHRTLKNSLRTLSNKY--DWIRAFPIVLLG*RNTYSDTTKASPAQMLFGTSTTIPVNLLHNPEPPTSNELD----FTRFNLRAQD 1891
            +G  YIL + D   K PEA  + D  A ++ +        RYG    ++ DQGT F S+I + ++  +    +RT+ YH + NGL ER++ TL   L    ++Y  DW     + LL  R +    TK SP ++++G    +P++L +  +PP S  ++     T+F  R Q+
Sbjct: 2631 NGNIYILVVTDYLTKFPEAFAMKDQKAKTIAQKLVEEICCRYGTPKQILTDQGTNFMSEIMKEVTNYFKIAKLRTSPYHPQCNGLTERFNGTLIQMLSNYVDRYQKDWDEYINLCLLAYRMSIHAATKMSPFKLMYGRECNMPMDLEY--QPPISQYMEEDDYVTKFKERMQE 2801

[Close]

BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Planmine SMEST
Match: SMESG000019863.1 (SMESG000019863.1)

HSP 1 Score: 131.339 bits (329), Expect = 5.344e-31
Identity = 74/209 (35.41%), Postives = 114/209 (54.55%), Query Frame = 2

Query:  455 IAESEFREIEKMEIIHRSNSPWASALYMVRKTNGSYQPCGYYRRLNYKTIPDAYPMPLIHDIVNRFEGCFIFFKL--------VPVAPENIPKTAITIPFALFECLCIPFGLRNATQTFQRLIDQVFVDLPYMAVYVDDIIIGSDSCVNHIKHLSEVFRPLLNHNLGINQTKCLFAKDSVSFLGHIISGSGLKPLPEKVNTVKTFPNLK 1057
            +AE E +++    +I  S SPW   + MV+K +G+ + C  YRRLN  T  D YP+P I++++   +G   F  L        + V  E+  KTA TI   L++   +PFGL NA  +FQR ++ V   +    VY+DD++I S +   H+K +  VF  L    L +  +KC +AK+ V+FLGHI+S  G +P P  +  +K  P  K
Sbjct: 2731 VAEKEVQKMLDAGVIEPSCSPWQFPVVMVKKKDGTQRFCIDYRRLNAVTKRDTYPLPDINEMLQMLDGAAYFTSLDLKSGYWQIKVKEEDREKTAFTIGKDLYQFKKMPFGLTNAPASFQRCMNFVTHGIKQCMVYIDDLLIYSSTFEQHLKDIRNVFVRLRQWKLKLKPSKCDWAKEKVTFLGHIVSAKGKEPDPRNIEKIKNCPAPK 2939

HSP 2 Score: 90.1225 bits (222), Expect = 4.275e-18
Identity = 54/182 (29.67%), Postives = 88/182 (48.35%), Query Frame = 2

Query: 1382 QPSSGYRYILTMMDRK*K*PEAVPLADFIANSVVRTFFSTWIARYGVSVHVIKDQGTQFESDIFRNLSKSYGFKLVRTTAYHSKTNGLIERYHRTLKNSLRTLSNKY--DWIRAFPIVLLG*RNTYSDTTKASPAQMLFGTSTTIPVNLLHNPEPPTSNELD--FTRFNLRAQD---TNPLH 1906
            + + G  YIL + D   K PEA  + D  A ++ R        RYG    ++ DQGT F S+I   ++  +    +RT+ YH +TNG  ER++  L   +    +++  DW R   + L+  R +    TK SP ++++G    +P++L + P      E+D   T F  R Q+   T  LH
Sbjct: 3259 ETNDGNIYILVVTDYLSKFPEAFAIPDQKARTIARILVEEICCRYGTPKQILTDQGTNFMSEIIEEVTNYFRIAKLRTSPYHPQTNGQTERFNGILIEMMSNYVSRHQKDWDRYINLCLMAYRMSIHSATKMSPFKLMYGRECNMPIDLEYQPPISPYMEIDDYVTGFKERMQEIWKTAELH 3440

The following BLAST results are available for this feature:

BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Human
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Celegans
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Fly
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Zebrafish
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Xenopus
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Mouse
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. UniProt/SwissProt
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. TrEMBL
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Cavefish
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Sea Lamprey
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Yeast
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Nematostella
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Medaka
BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Planmine SMEST

BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
BX511224.1	9.346e-34	32.43	pep chromosome:GRCz11:2:18017000:18022765:1 gene:E...	[more]
BX546500.1	1.160e-32	38.07	pep chromosome:GRCz11:23:12926092:12931693:-1 gene...	[more]
CR925755.2	2.721e-32	37.56	pep chromosome:GRCz11:17:42486740:42492668:-1 gene...	[more]
BX511082.1	4.787e-32	38.07	pep chromosome:GRCz11:9:14291932:14297132:1 gene:E...	[more]
CR855320.1	5.403e-31	37.06	pep chromosome:GRCz11:1:7956030:7961696:1 gene:ENS...	[more]

BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
anxa6	5.701e-34	30.77	annexin A6 [Source:Xenbase;Acc:XB-GENE-989741]	[more]
ENSXETT00000006041.1	3.953e-30	36.27	pep primary_assembly:Xenopus_tropicalis_v9.1:6:373...	[more]
lin54	1.909e-27	34.02	lin-54 DREAM MuvB core complex component [Source:X...	[more]
castor1	2.040e-27	34.02	cytosolic arginine sensor for mTORC1 subunit 1 [So...	[more]
ENSXETT00000020886.1	2.040e-27	34.02	pep primary_assembly:Xenopus_tropicalis_v9.1:3:496...	[more]

BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|Q99315\|YG31B_YEAST	1.834e-29	37.50	Transposon Ty3-G Gag-Pol polyprotein OS=Saccharomy...	[more]
sp\|Q7LHG5\|YI31B_YEAST	1.953e-29	37.50	Transposon Ty3-I Gag-Pol polyprotein OS=Saccharomy...	[more]
sp\|P04323\|POL3_DROME	1.200e-28	33.65	Retrovirus-related Pol polyprotein from transposon...	[more]
sp\|P20825\|POL2_DROME	1.201e-28	36.49	Retrovirus-related Pol polyprotein from transposon...	[more]
sp\|P10401\|POLY_DROME	4.604e-26	34.72	Retrovirus-related Pol polyprotein from transposon...	[more]

BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A4Y2W1J5	1.699e-89	36.86	Retrovirus-related Pol polyprotein from transposon...	[more]
A0A085ND72	2.565e-78	32.75	Uncharacterized protein OS=Trichuris suis OX=68888...	[more]
X1WR82	1.076e-67	32.07	Uncharacterized protein OS=Acyrthosiphon pisum OX=...	[more]
A0A0C2NLD3	7.101e-60	41.61	Transposon Ty3-I Gag-Pol polyprotein OS=Thelohanel...	[more]
A0A418XSS2	8.111e-60	48.79	RNA-directed DNA polymerase OS=Alcanivorax profund...	[more]

BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ENSAMXT00000041345.1	2.477e-40	33.11	pep primary_assembly:Astyanax_mexicanus-2.0:25:323...	[more]
ENSAMXT00000029916.1	6.292e-36	27.91	pep primary_assembly:Astyanax_mexicanus-2.0:10:193...	[more]
ENSAMXT00000054230.1	7.784e-35	37.20	pep primary_assembly:Astyanax_mexicanus-2.0:14:194...	[more]
ENSAMXT00000045469.1	1.129e-34	38.24	pep primary_assembly:Astyanax_mexicanus-2.0:APWO02...	[more]
ENSAMXT00000041754.1	1.901e-34	39.69	pep primary_assembly:Astyanax_mexicanus-2.0:APWO02...	[more]

BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ENSORLT00000040051.1	2.485e-38	34.20	pep primary_assembly:ASM223467v1:1:37354481:373592...	[more]
ENSORLT00000045600.1	4.973e-34	36.71	pep primary_assembly:ASM223467v1:4:26167161:261711...	[more]
ENSORLT00000045141.1	1.039e-33	39.11	pep primary_assembly:ASM223467v1:23:22495254:22500...	[more]
ENSORLT00000040501.1	1.192e-33	36.71	pep primary_assembly:ASM223467v1:1:1251188:1255180...	[more]
ENSORLT00000045829.1	2.920e-32	42.22	pep primary_assembly:ASM223467v1:10:30089555:30096...	[more]

BLAST of Transposon Ty3-I Gag-Pol polyprotein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000011773.1	1.130e-42	50.00	SMESG000011773.1	[more]
SMESG000037879.1	1.261e-39	38.05	SMESG000037879.1	[more]
SMESG000080881.1	8.584e-32	36.89	SMESG000080881.1	[more]
SMESG000038549.1	4.202e-31	31.94	SMESG000038549.1	[more]
SMESG000019863.1	5.344e-31	35.41	SMESG000019863.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30019956 ID=SMED30019956|Name=Transposon Ty3-I Gag-Pol polyprotein|organism=Schmidtea mediterranea sexual|type=transcript|length=2143bp
TGTATATTTCATGATAGAAACAATGCCGATTCTGTCATTGAGAGCGAGTT
ATCAGCAGCAAATGGCTCTTTGATCCTCATATATGGCCAATTACGAATTC
GCTTCCAATTTCCACCAATTCCAAATGATTTTGTTTAGACTTTTTCTGTA
CCAACCAATAATCGGTTCAAATTTTCTTGCTCACAATGGATTTTGTCGTT
GATTGTTAATCCAGATCACTAATCACGTCGAATGTATCAGTCAGAGTTCC
GACAACCAAAATTCATCAACCTTCAAGCATACTATGACAACTAGGGTCTA
TCACCAATAAAAATGTTGAAATAATTTCATGGAACTTATTACCGAACCAA
TTTACCAGACTTTTAGAAGATGACTTACCAGCAATATCAACAACTGTTGC
TCAGCATATCATTCAAACCAATCCTGACCCATCTCAGTTTACTAGACGTC
GCGAATTGCAGAGTCAGAATTTCGAGAGATAGAAAAAATGGAAATTATAC
ACAGATCAAATTCACCATGGGCATCGGCATTATACATGGTACGCAAAACC
AACGGATCTTATCAGCCTTGTGGCTACTATAGAAGATTAAATTATAAGAC
AATACCTGATGCTTATCCGATGCCTCTCATTCATGATATTGTGAACCGAT
TTGAAGGATGCTTTATTTTTTTTAAGCTTGTACCAGTGGCACCCGAAAAC
ATTCCAAAGACTGCTATAACAATTCCTTTCGCTTTATTTGAATGTCTTTG
TATTCCTTTTGGATTGCGTAATGCGACACAAACTTTTCAGAGGCTCATAG
ACCAAGTGTTTGTGGATCTTCCCTATATGGCAGTCTACGTCGATGACATC
ATCATAGGATCCGACTCATGCGTCAATCACATTAAGCATTTGTCAGAGGT
TTTTCGTCCACTTTTAAATCATAATCTAGGCATAAATCAGACCAAATGTC
TGTTTGCCAAGGACTCGGTATCTTTCCTTGGTCATATTATCAGTGGATCC
GGATTAAAACCTCTACCAGAAAAAGTCAATACTGTCAAAACTTTCCCAAA
CCTCAAATTCTAACACAATTACGCAGCTTTGTAGGTATCATCAACTTTTA
TCATCAATTTATTAAGAAGAGGAATTTATTAATACAAAGAGGCGTTGGAA
GACGCGGTAGCACTAGCGTCTCCAGCAGAAAATGCAGAATAAAATCTTAA
AGCCGACACCTCTGACGTAGCCATAGGAGCATAATTACATCAAGTCATCG
ATGGGAAAAGATCCCACTGGCGTTTTTTTCACGAAAGCTCACTCAATGCT
AACGGAAATATTCGACATTCGACAGAGAGCTCTTGGGAATCACTGAATCA
ATAAAACATTTTCAGTATATGTATATCACTTCAGCCAAGCTCAGGTTATC
GTTATATTTTGACTATGATGGATAGGAAGTAAAAATGACCAGAAGCAGTT
CCACTAGCTGATTTTATTGCAAATTCCGTCGTTCGAACCTTCTTTAGTAC
TTGGATTGCTAGATACGGTGTGTCAGTACATGTGATTAAGGATCAAGGCA
CTCAATTCGAATCTGATATATTCAGAAACCTATCAAAGTCATACGGTTTT
AAACTAGTTCGAACCACAGCCTATCACTCCAAAACGAATGGACTAATCGA
GAGGTATCATCGCACCTTAAAGAACTCACTGAGGACACTGTCAAATAAAT
ACGATTGGATAAGAGCATTTCCAATAGTATTGCTAGGGTAGAGAAACACA
TATTCCGATACAACTAAAGCTTCTCCAGCGCAAATGTTATTTGGCACTTC
GACAACAATACCAGTAAATCTTTTACATAATCCAGAACCACCAACGTCAA
ACGAATTAGACTTCACAAGATTTAATCTTAGAGCCCAGGATACGAACCCA
CTTCACTCGACAGTTAACACTACTGATTTTGCCAGAGGGTTCGATCTGTG
CAAGATACTTCCCTCTCACCAAGATATCATAGACCTTAGAAGCTCAAAGA
AATAAAAAACAGCACAGCTATAATCGAAACACATCAAGGAGCAACAACAG
TTAATTTTGCAAGACTTAAACCTGCCTTTTGTCGAAGAGAATTACCAGTA
GAGAATTACCAGAAGAGAATTATGTTAATGCTCCACATCTTGA

protein sequence of SMED30019956-orf-1

>SMED30019956-orf-1 ID=SMED30019956-orf-1|Name=SMED30019956-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=192bp
MEIIHRSNSPWASALYMVRKTNGSYQPCGYYRRLNYKTIPDAYPMPLIHD
IVNRFEGCFIFFKLVPVAPENIPKTAITIPFALFECLCIPFGLRNATQTF
QRLIDQVFVDLPYMAVYVDDIIIGSDSCVNHIKHLSEVFRPLLNHNLGIN
QTKCLFAKDSVSFLGHIISGSGLKPLPEKVNTVKTFPNLKF*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **INTERPRO**
Term	Definition
IPR043128	Rev_trsase/Diguanyl_cyclase
IPR012337	RNaseH-like_sf
IPR001584	Integrase_cat-core
IPR000477	RT_dom

Vocabulary: **molecular function**
Term	Definition
GO:0003676	nucleic acid binding
GO:0004190	aspartic-type endopeptidase activity

Vocabulary: **biological process**
Term	Definition
GO:0015074	DNA integration
GO:0006508	proteolysis

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000477	Reverse transcriptase domain	PFAM	PF00078	RVT_1	coord: 82..168 e-value: 8.4E-14 score: 51.6
IPR043128	Reverse transcriptase/Diguanylate cyclase domain	GENE3D	G3DSA:3.30.70.270		coord: 43..168 e-value: 3.2E-48 score: 165.9
None	No IPR available	GENE3D	G3DSA:3.10.10.10		coord: 2..95 e-value: 3.2E-48 score: 165.9
None	No IPR available	PANTHER	PTHR24559:SF306		coord: 6..136
None	No IPR available	PANTHER	PTHR24559	FAMILY NOT NAMED	coord: 6..136
None	No IPR available	CDD	cd01647	RT_LTR	coord: 3..168 e-value: 2.98635E-59 score: 181.254
None	No IPR available	SUPERFAMILY	SSF56672	DNA/RNA polymerases	coord: 2..188

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Transposon Ty3-I Gag-Pol polyprotein

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 2

Owlery

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