Granulin precursor
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30019945 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 10Note: Hover over icons to view figure legend
Homology
BLAST of Granulin precursor vs. Ensembl Human
Match: GRN (granulin precursor [Source:HGNC Symbol;Acc:HGNC:4601]) HSP 1 Score: 90.5077 bits (223), Expect = 1.747e-20 Identity = 59/191 (30.89%), Postives = 83/191 (43.46%), Query Frame = 1
Query: 52 LIFVVKIYADGVMSFDVLLENQKN-----VTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVE 609
L+ I G L Q+ +++ +CP+ R++C D TCC+ Y CC NA CC D HCCP+ + CD Q KC+ + ++ +PA V D V + EV CP+ T C+ + CCP AVCC D CCP G TCD + C + V +E
Sbjct: 173 LVHTRCITPTGTHPLAKKLPAQRTNRAVALSSSVMCPDARSRCPDGSTCCELPSGKYGCCPMPNATCCSDHLHCCPQDTVCDLIQSKCLSKENATTDLLTKLPAHTVGD----------VKCDMEVSCPDGYTCCRLQS-------GAWGCCPFTQAVCCEDHIHCCPAGFTCDTQKGTCEQGPHQVPWME 346 HSP 2 Score: 87.0409 bits (214), Expect = 2.305e-19 Identity = 61/188 (32.45%), Postives = 85/188 (45.21%), Query Frame = 1
Query: 76 ADGVMSFDVLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVELPNDAEENGN 639
ADG F + N CP+ + +C D TCC S+ CC A CC D+ HCCP G+ CD +C+ + K +PA + + V + VMCP+ +++C D TCC Y CCP NA CCSD CCP+ T CD+ KCL + ++ A G+
Sbjct: 107 ADGRSCFQ---RSGNNSVGAIQCPDSQFECPDFSTCCVMVDGSWGCCPMPQASCCEDRVHCCPHGAFCDLVHTRCIT-PTGTHPLAKKLPAQRTNR---------AVALSSSVMCPDARSRCPDGSTCCELPSGKYGCCPMPNATCCSDHLHCCPQDTVCDLIQSKCLSKENATTDLLTKLPAHTVGD 281 HSP 3 Score: 80.1073 bits (196), Expect = 6.482e-17 Identity = 53/176 (30.11%), Postives = 80/176 (45.45%), Query Frame = 1
Query: 97 DVLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVELPNDA 624
D+L + + D C + C D TCC+ + ++ CC + AVCC D HCCP G TCD ++ C + M K L + P L+ V ++ CP++ TCC + CCP++ VCC+D+ CCP G C + KCL+ + L + A
Sbjct: 269 DLLTKLPAHTVGDVKC-DMEVSCPDGYTCCRLQSGAWGCCPFTQAVCCEDHIHCCPAGFTCDTQKGTCEQGPHQVPWMEKAPAHLSL---PDPQALKRDVPCDNVSSCPSS-------DTCCRDNRQGWACCPYRQGVCCADRRHCCPAGFRCAARGTKCLRREAPRWDAPLRDPA 433 HSP 4 Score: 54.6842 bits (130), Expect = 3.593e-8 Identity = 39/142 (27.46%), Postives = 59/142 (41.55%), Query Frame = 1
Query: 163 CTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSS 588
C+ +C T + CC + AV C D HCCP G C A C + + N + CP+++ +C D TCC+ ++ CCP A CC D+ CCP G CD+ + +C+ +
Sbjct: 67 CSAGHSCIFTVSGTSSCCPFPEAVACGDGHHCCPRGFHCSADGRSCFQ--------------------------RSGNNSVGAIQCPDSQFECPDFSTCCVMVDGSWGCCPMPQASCCEDRVHCCPHGAFCDLVHTRCITPT 182
BLAST of Granulin precursor vs. Ensembl Human
Match: GRN (granulin precursor [Source:HGNC Symbol;Acc:HGNC:4601]) HSP 1 Score: 89.7373 bits (221), Expect = 2.099e-20 Identity = 54/162 (33.33%), Postives = 76/162 (46.91%), Query Frame = 1
Query: 124 VTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVE 609
+++ +CP+ R++C D TCC+ Y CC NA CC D HCCP+ + CD Q KC+ + ++ +PA V D V + EV CP+ T C+ + CCP AVCC D CCP G TCD + C + V +E
Sbjct: 16 LSSSVMCPDARSRCPDGSTCCELPSGKYGCCPMPNATCCSDHLHCCPQDTVCDLIQSKCLSKENATTDLLTKLPAHTVGD----------VKCDMEVSCPDGYTCCRLQS-------GAWGCCPFTQAVCCEDHIHCCPAGFTCDTQKGTCEQGPHQVPWME 160 HSP 2 Score: 77.0258 bits (188), Expect = 7.297e-16 Identity = 55/173 (31.79%), Postives = 81/173 (46.82%), Query Frame = 1
Query: 91 SFDVLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVE 609
+ D+L + + D C + C D TCC+ + ++ CC + AVCC D HCCP G TCD ++ C + M K L + P L+ V ++ CP++ T C+ T + CCP AVCCSD CCP+G TC + +C + S V +E
Sbjct: 81 TTDLLTKLPAHTVGDVKC-DMEVSCPDGYTCCRLQSGAWGCCPFTQAVCCEDHIHCCPAGFTCDTQKGTCEQGPHQVPWMEKAPAHLSL---PDPQALKRDVPCDNVSSCPSSDTCCQLTS-------GEWGCCPIPEAVCCSDHQHCCPQGYTC-VAEGQCQRGSEIVAGLE 241 HSP 3 Score: 65.855 bits (159), Expect = 4.732e-12 Identity = 49/176 (27.84%), Postives = 76/176 (43.18%), Query Frame = 1
Query: 115 QKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVELPNDAEENGNK 642
+++V DN+ + C TCCQ + CC AVCC D HCCP+G TC A + +C + ++ + K+ P++ + + CP +T C ++ CC +AVCC D+ CCP G TC++K + C K S + G K
Sbjct: 175 KRDVPCDNV-----SSCPSSDTCCQLTSGEWGCCPIPEAVCCSDHQHCCPQGYTCVA-EGQCQRGSEIVAGLEKMPARRASLSHPRD------IGCDQHTSCPVGQTCCPSLG-------GSWACCQLPHAVCCEDRQHCCPAGYTCNVKARSCEKEVVSAQPATFLARSPHVGVK 331 HSP 4 Score: 52.373 bits (124), Expect = 2.004e-7 Identity = 29/86 (33.72%), Postives = 42/86 (48.84%), Query Frame = 1
Query: 148 NKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNEN 405
++ T C QTCC + S+ CC+ +AVCC D+ HCCP G TC+ K C K E+ PA + P + + + E
Sbjct: 259 DQHTSCPVGQTCCPSLGGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARSCEK------EVVSAQPATFLARSPHVGVKDVECGEG 338
BLAST of Granulin precursor vs. Ensembl Human
Match: GRN (granulin precursor [Source:HGNC Symbol;Acc:HGNC:4601]) HSP 1 Score: 90.5077 bits (223), Expect = 2.227e-20 Identity = 59/191 (30.89%), Postives = 83/191 (43.46%), Query Frame = 1
Query: 52 LIFVVKIYADGVMSFDVLLENQKN-----VTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVE 609
L+ I G L Q+ +++ +CP+ R++C D TCC+ Y CC NA CC D HCCP+ + CD Q KC+ + ++ +PA V D V + EV CP+ T C+ + CCP AVCC D CCP G TCD + C + V +E
Sbjct: 173 LVHTRCITPTGTHPLAKKLPAQRTNRAVALSSSVMCPDARSRCPDGSTCCELPSGKYGCCPMPNATCCSDHLHCCPQDTVCDLIQSKCLSKENATTDLLTKLPAHTVGD----------VKCDMEVSCPDGYTCCRLQS-------GAWGCCPFTQAVCCEDHIHCCPAGFTCDTQKGTCEQGPHQVPWME 346 HSP 2 Score: 87.0409 bits (214), Expect = 3.457e-19 Identity = 61/188 (32.45%), Postives = 85/188 (45.21%), Query Frame = 1
Query: 76 ADGVMSFDVLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVELPNDAEENGN 639
ADG F + N CP+ + +C D TCC S+ CC A CC D+ HCCP G+ CD +C+ + K +PA + + V + VMCP+ +++C D TCC Y CCP NA CCSD CCP+ T CD+ KCL + ++ A G+
Sbjct: 107 ADGRSCFQ---RSGNNSVGAIQCPDSQFECPDFSTCCVMVDGSWGCCPMPQASCCEDRVHCCPHGAFCDLVHTRCIT-PTGTHPLAKKLPAQRTNR---------AVALSSSVMCPDARSRCPDGSTCCELPSGKYGCCPMPNATCCSDHLHCCPQDTVCDLIQSKCLSKENATTDLLTKLPAHTVGD 281 HSP 3 Score: 78.5666 bits (192), Expect = 2.588e-16 Identity = 48/164 (29.27%), Postives = 74/164 (45.12%), Query Frame = 1
Query: 148 NKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNE-----NDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVELPNDA 624
++ T C QTCC + S+ CC+ +AVCC D+ HCCP G TC+ K C K E+ PA + P + + + E +++ C +N + CCP++ VCC+D+ CCP G C + KCL+ + L + A
Sbjct: 445 DQHTSCPVGQTCCPSLGGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARSCEK------EVVSAQPATFLARSPHVGVKDVECGEGHFCHDNQTCCRDN--------------RQGWACCPYRQGVCCADRRHCCPAGFRCAARGTKCLRREAPRWDAPLRDPA 588 HSP 4 Score: 77.0258 bits (188), Expect = 9.237e-16 Identity = 55/171 (32.16%), Postives = 80/171 (46.78%), Query Frame = 1
Query: 97 DVLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVE 609
D+L + + D C + C D TCC+ + ++ CC + AVCC D HCCP G TCD ++ C + M K L + P L+ V ++ CP++ T C+ T + CCP AVCCSD CCP+G TC + +C + S V +E
Sbjct: 269 DLLTKLPAHTVGDVKC-DMEVSCPDGYTCCRLQSGAWGCCPFTQAVCCEDHIHCCPAGFTCDTQKGTCEQGPHQVPWMEKAPAHLSL---PDPQALKRDVPCDNVSSCPSSDTCCQLTS-------GEWGCCPIPEAVCCSDHQHCCPQGYTC-VAEGQCQRGSEIVAGLE 427 HSP 5 Score: 66.6254 bits (161), Expect = 3.698e-12 Identity = 51/179 (28.49%), Postives = 78/179 (43.58%), Query Frame = 1
Query: 115 QKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKL--IPALKVD-DEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVELPNDAEENGNK 642
+++V DN+ + C TCCQ + CC AVCC D HCCP+G TC A+ +C + + + L +PA + P++ + + CP +T C ++ CC +AVCC D+ CCP G TC++K + C K S + G K
Sbjct: 361 KRDVPCDNV-----SSCPSSDTCCQLTSGEWGCCPIPEAVCCSDHQHCCPQGYTCVAEG----QCQRGSEIVAGLEKMPARRASLSHPRD------IGCDQHTSCPVGQTCCPSLG-------GSWACCQLPHAVCCEDRQHCCPAGYTCNVKARSCEKEVVSAQPATFLARSPHVGVK 517 HSP 6 Score: 53.9138 bits (128), Expect = 5.712e-8 Identity = 41/142 (28.87%), Postives = 61/142 (42.96%), Query Frame = 1
Query: 163 CTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSS 588
C+ +C T + CC + AV C D HCCP G C A C + NN + A + CP+++ +C D TCC+ ++ CCP A CC D+ CCP G CD+ + +C+ +
Sbjct: 67 CSAGHSCIFTVSGTSSCCPFPEAVACGDGHHCCPRGFHCSADGRSCFQ-------------------RSGNNSVGA-------IQCPDSQFECPDFSTCCVMVDGSWGCCPMPQASCCEDRVHCCPHGAFCDLVHTRCITPT 182
BLAST of Granulin precursor vs. Ensembl Human
Match: GRN (granulin precursor [Source:HGNC Symbol;Acc:HGNC:4601]) HSP 1 Score: 78.5666 bits (192), Expect = 2.167e-16 Identity = 48/164 (29.27%), Postives = 74/164 (45.12%), Query Frame = 1
Query: 148 NKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNE-----NDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVELPNDA 624
++ T C QTCC + S+ CC+ +AVCC D+ HCCP G TC+ K C K E+ PA + P + + + E +++ C +N + CCP++ VCC+D+ CCP G C + KCL+ + L + A
Sbjct: 288 DQHTSCPVGQTCCPSLGGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARSCEK------EVVSAQPATFLARSPHVGVKDVECGEGHFCHDNQTCCRDN--------------RQGWACCPYRQGVCCADRRHCCPAGFRCAARGTKCLRREAPRWDAPLRDPA 431 HSP 2 Score: 77.7962 bits (190), Expect = 3.676e-16 Identity = 59/178 (33.15%), Postives = 80/178 (44.94%), Query Frame = 1
Query: 76 ADGVMSFDVLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVE 609
ADG F + N CP+ + +C D TCC S+ CC AVCC D HCCP G TCD ++ C + M K L + P L+ V ++ CP++ T C+ T + CCP AVCCSD CCP+G TC + +C + S V +E
Sbjct: 107 ADGRSCFQ---RSGNNSVGAIQCPDSQFECPDFSTCCVMVDGSWGCCPMPQAVCCEDHIHCCPAGFTCDTQKGTCEQGPHQVPWMEKAPAHLSL---PDPQALKRDVPCDNVSSCPSSDTCCQLTS-------GEWGCCPIPEAVCCSDHQHCCPQGYTC-VAEGQCQRGSEIVAGLE 270 HSP 3 Score: 66.2402 bits (160), Expect = 3.533e-12 Identity = 51/179 (28.49%), Postives = 78/179 (43.58%), Query Frame = 1
Query: 115 QKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKL--IPALKVD-DEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVELPNDAEENGNK 642
+++V DN+ + C TCCQ + CC AVCC D HCCP+G TC A+ +C + + + L +PA + P++ + + CP +T C ++ CC +AVCC D+ CCP G TC++K + C K S + G K
Sbjct: 204 KRDVPCDNV-----SSCPSSDTCCQLTSGEWGCCPIPEAVCCSDHQHCCPQGYTCVAEG----QCQRGSEIVAGLEKMPARRASLSHPRD------IGCDQHTSCPVGQTCCPSLG-------GSWACCQLPHAVCCEDRQHCCPAGYTCNVKARSCEKEVVSAQPATFLARSPHVGVK 360 HSP 4 Score: 56.6102 bits (135), Expect = 7.479e-9 Identity = 45/149 (30.20%), Postives = 62/149 (41.61%), Query Frame = 1
Query: 163 CTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVE 609
C+ +C T + CC + AV C D HCCP G C A C + NN + A + CP+++ +C D TCC+ ++ CCP AVCC D CCP G TCD + C + V +E
Sbjct: 67 CSAGHSCIFTVSGTSSCCPFPEAVACGDGHHCCPRGFHCSADGRSCFQ-------------------RSGNNSVGA-------IQCPDSQFECPDFSTCCVMVDGSWGCCPMPQAVCCEDHIHCCPAGFTCDTQKGTCEQGPHQVPWME 189
BLAST of Granulin precursor vs. Ensembl Human
Match: GRN (granulin precursor [Source:HGNC Symbol;Acc:HGNC:4601]) HSP 1 Score: 49.6766 bits (117), Expect = 4.596e-7 Identity = 36/116 (31.03%), Postives = 55/116 (47.41%), Query Frame = 1
Query: 163 CTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAV 510
C+ +C T + CC + A CC D+ HCCP G+ CD +C+ ++ + K +PA + + V + VMCP+ +++C D TCC Y CCP NA
Sbjct: 67 CSAGHSCIFTVSGTSSCCPFPEASCCEDRVHCCPHGAFCDLVHTRCITPTGTH-PLAKKLPAQRTNRA---------VALSSSVMCPDARSRCPDGSTCCELPSGKYGCCPMPNAT 172
BLAST of Granulin precursor vs. Ensembl Celegans
Match: pgrn-1 (ProGRaNulin homolog [Source:UniProtKB/TrEMBL;Acc:Q7JKP2]) HSP 1 Score: 96.6709 bits (239), Expect = 1.618e-23 Identity = 66/174 (37.93%), Postives = 95/174 (54.60%), Query Frame = 1
Query: 34 LGFNLLLIFVVKIYADGVMSFDVLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTC 555
L + IF +KI ++ V L T + C + T+C+D +TCC+ N++ CC NAVCC D+ HCCP G+TCD + +C+ + +V M K PA K + N+ +EV+CP+ +KC D TCC+ + +Y CCP NAVCC+D CCP G TC
Sbjct: 66 LHSSFSFIFSIKIMRLILLFLAVFLCR----VTAHQC-DAETECSDDETCCKLGDNTWGCCPMPNAVCCDDRSHCCPTGTTCDPQGARCIGADEKHVPMKKKKPARKTVER----------NQFNEVVCPDKASKCPDGSTCCLLEQGSYGCCPVPNAVCCADMLHCCPNGFTC 224 HSP 2 Score: 60.4622 bits (145), Expect = 1.273e-10 Identity = 27/58 (46.55%), Postives = 35/58 (60.34%), Query Frame = 1
Query: 115 QKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAK 288
++N + +CP+K +KC D TCC + SY CC NAVCC D HCCP G TC +
Sbjct: 170 ERNQFNEVVCPDKASKCPDGSTCCLLEQGSYGCCPVPNAVCCADMLHCCPNGFTCHGQ 227 HSP 3 Score: 46.9802 bits (110), Expect = 4.124e-6 Identity = 19/37 (51.35%), Postives = 25/37 (67.57%), Query Frame = 1
Query: 475 NNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKS 585
N + CCP NAVCC D+ CCP GTTCD + +C+ +
Sbjct: 116 NTWGCCPMPNAVCCDDRSHCCPTGTTCDPQGARCIGA 152
BLAST of Granulin precursor vs. Ensembl Celegans
Match: pgrn-1 (ProGRaNulin homolog [Source:UniProtKB/TrEMBL;Acc:Q7JKP2]) HSP 1 Score: 96.6709 bits (239), Expect = 1.768e-23 Identity = 65/171 (38.01%), Postives = 94/171 (54.97%), Query Frame = 1
Query: 43 NLLLIFVVKIYADGVMSFDVLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTC 555
+ IF +KI ++ V L T + C + T+C+D +TCC+ N++ CC NAVCC D+ HCCP G+TCD + +C+ + +V M K PA K + N+ +EV+CP+ +KC D TCC+ + +Y CCP NAVCC+D CCP G TC
Sbjct: 69 SFSFIFSIKIMRLILLFLAVFLCR----VTAHQC-DAETECSDDETCCKLGDNTWGCCPMPNAVCCDDRSHCCPTGTTCDPQGARCIGADEKHVPMKKKKPARKTVER----------NQFNEVVCPDKASKCPDGSTCCLLEQGSYGCCPVPNAVCCADMLHCCPNGFTC 224 HSP 2 Score: 47.3654 bits (111), Expect = 2.714e-6 Identity = 19/37 (51.35%), Postives = 25/37 (67.57%), Query Frame = 1
Query: 475 NNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKS 585
N + CCP NAVCC D+ CCP GTTCD + +C+ +
Sbjct: 116 NTWGCCPMPNAVCCDDRSHCCPTGTTCDPQGARCIGA 152
BLAST of Granulin precursor vs. Ensembl Celegans
Match: T02B11.8 (pep chromosome:WBcel235:V:877530:878667:-1 gene:WBGene00044776.1 transcript:T02B11.8a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:T02B11.8) HSP 1 Score: 43.8986 bits (102), Expect = 5.415e-6 Identity = 26/82 (31.71%), Postives = 34/82 (41.46%), Query Frame = 1
Query: 43 NLLLIFVVKIYADGVMSFDVLLENQKNVTTDNICPNKRT-KCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDA 285
N LIF + F + K N+C KRT +C + CC + Y CC + CCP HCCP G +C
Sbjct: 2 NYFLIFSI---------FVLQFHLSKCELEPNLCDEKRTTQCDEYAVCCPIGNEQYGCCPFTGGTCCPGTNHCCPPGFSCTT 74
BLAST of Granulin precursor vs. Ensembl Zebrafish
Match: grnb (granulin b [Source:ZFIN;Acc:ZDB-GENE-030131-7393]) HSP 1 Score: 105.531 bits (262), Expect = 9.267e-26 Identity = 63/150 (42.00%), Postives = 87/150 (58.00%), Query Frame = 1
Query: 139 ICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNI-LEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKS 585
+CP+ ++C D TCCQ + CC NAVCC D+ HCCP+G+TCD CV + + A + KN + L A+VN EV+CP+ +KC + TCC+ + +Y CCP AVCCSD+ CCPEGTTCD+ + CL +
Sbjct: 94 VCPDGESECPDDTTCCQMPDGGWGCCPMKNAVCCDDRKHCCPQGTTCDLVHSMCVSTTYGSSPFLRKFDARRRKPLEKNAVDLPAEVNNIREVICPDKISKCPEDTTCCLLETGSYGCCPMPKAVCCSDQKHCCPEGTTCDLIHSTCLSA 243 HSP 2 Score: 92.4337 bits (228), Expect = 2.889e-21 Identity = 63/159 (39.62%), Postives = 82/159 (51.57%), Query Frame = 1
Query: 100 VLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKC 576
V L + N + ICP+K +KC + TCC + SY CC AVCC D+ HCCPEG+TCD C+ A EM IPA+ V +PK ++ N+ V C + TCC T ++ CCP AVCC D CCPEGT C++ C
Sbjct: 174 VDLPAEVNNIREVICPDKISKCPEDTTCCLLETGSYGCCPMPKAVCCSDQKHCCPEGTTCDLIHSTCLS-ANGVSEMATKIPAVTV-LKPKEEVVPC----NETVACSSG-------TTCCKTPEGSWACCPLPKAVCCEDHIHCCPEGTLCNVAASSC 319 HSP 3 Score: 86.2705 bits (212), Expect = 3.715e-19 Identity = 65/181 (35.91%), Postives = 89/181 (49.17%), Query Frame = 1
Query: 91 SFDVLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVELPNDAEEN 633
+F LL N T ICP+ C D TCC T Y CC +A CC D HCC +G+ CD + KCV K++V L KV+ + L+A V + E CP++ TCC + CCP KNAVCC D+ CCP+GTTCD+ + C+ +++ + DA
Sbjct: 5 AFIALLCVCVNACTALICPDGGM-CEDGNTCCLTPSGGYGCCPLPHAECCSDHLHCCYQGTLCDLEHSKCVN--KTHV----LDWVEKVEAK-----LQAVVCPDGESECPDD-------TTCCQMPDGGWGCCPMKNAVCCDDRKHCCPQGTTCDLVHSMCVSTTYGSSPFLRKFDARRR 166 HSP 4 Score: 83.1889 bits (204), Expect = 4.769e-18 Identity = 60/190 (31.58%), Postives = 83/190 (43.68%), Query Frame = 1
Query: 52 LIFVVKIYADGVMSFDVLLENQKNVTTDNICP------NKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKS----NVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSF 591
L V D V + + V+T I P ++ + C TCC+ + CC AVCC D HCCP GS C+ C + S +V M K IPA+ V + K N ++ CP T CK T ++ CCP AVCC+D+ CCP+G TCD+ C++S
Sbjct: 311 LCNVAASSCDDPAGLSVSVPWMEKVSTKPIAPPPNKKCDESSSCPGESTCCKLSSGDWGCCPLPEAVCCEDHVHCCPHGSVCNVAAETCETVSDSGLRISVPMVKKIPAVSVPSQ--------KQNCDETSSCPTGTTCCKLTS-------GSWACCPMPQAVCCADQEHCCPQGYTCDLAQSSCVRSGL 485 HSP 5 Score: 74.3294 bits (181), Expect = 4.796e-15 Identity = 59/196 (30.10%), Postives = 86/196 (43.88%), Query Frame = 1
Query: 37 GFNLLLIFVVKIYADGVMSFDVLLENQKNVTT-DNICP-NKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPAL-KVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVELP 615
G LI + A+GV + + + + P N+ C+ TCC+T S+ CC AVCC D HCCPEG+ C+ C A +V +P + KV +P K +E+ CP T CK + ++ CCP AVCC D CCP G+ C++ + C S S + +P
Sbjct: 230 GTTCDLIHSTCLSANGVSEMATKIPAVTVLKPKEEVVPCNETVACSSGTTCCKTPEGSWACCPLPKAVCCEDHIHCCPEGTLCNVAASSCDDPAGLSVS----VPWMEKVSTKPIAPPPNKKCDESSS--CPGESTCCKLSS-------GDWGCCPLPEAVCCEDHVHCCPHGSVCNVAAETCETVSDSGLRISVP 412 HSP 6 Score: 73.9442 bits (180), Expect = 7.702e-15 Identity = 57/191 (29.84%), Postives = 83/191 (43.46%), Query Frame = 1
Query: 25 VAILGFNLLLIFVVKIYADGVMSFDVLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSV 597
V+ G + + V KI A V S ++N + CP TCC+ S+ CC AVCC D+ HCCP+G TCD Q CV+ ++ + PAL+ + +E + + + CP + T C + + CCP AVCC D CCP G TC+ + C K +
Sbjct: 402 VSDSGLRISVPMVKKIPAVSVPS------QKQNCDETSSCPTG-------TTCCKLTSGSWACCPMPQAVCCADQEHCCPQGYTCDLAQSSCVRSGLPSMAWFRKEPALR-----ETQRVEDRHMCDAQTSCPRDDTCCFINRI------GKWGCCPLPKAVCCKDGDHCCPSGYTCNEEKTSCTKGLHQI 568 HSP 7 Score: 58.151 bits (139), Expect = 1.470e-9 Identity = 50/176 (28.41%), Postives = 72/176 (40.91%), Query Frame = 1
Query: 106 LENQKNVTTDNICPNKRTKCTDRQTCCQT-KHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKS----SFSVNNVELPN 618
L + V ++C + +T C TCC + + CC AVCC D HCCP G TC+ ++ C K K +V + V + CP+ T C + CCP AVCC D CCP+G C ++ C K+ S S+ V++P
Sbjct: 497 LRETQRVEDRHMC-DAQTSCPRDDTCCFINRIGKWGCCPLPKAVCCKDGDHCCPSGYTCNEEKTSCTKGLHQIPWFTKK--TARVWKSSDELLGHEDVKCDSSTSCPSGSTCCILPT-------GQWGCCPLVKAVCCEDHEHCCPQGYICKLELGTCEKASADVSVSLTAVQMPE 662 HSP 8 Score: 57.3806 bits (137), Expect = 2.989e-9 Identity = 50/180 (27.78%), Postives = 71/180 (39.44%), Query Frame = 1
Query: 52 LIFVVKIYADGVMSFDVLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKL---IPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLK 582
+ + K A S D LL ++V D+ T C TCC + CC AVCC D HCCP+G C + C K A ++V + +P ++ D + ++ D + + CCP+ AVCC D CCP G CD K + C K
Sbjct: 568 IPWFTKKTARVWKSSDELL-GHEDVKCDS-----STSCPSGSTCCILPTGQWGCCPLVKAVCCEDHEHCCPQGYICKLELGTCEK-ASADVSVSLTAVQMPEIQCDTFTRCAHTQSCCRLAD----------------------STWACCPYTQAVCCKDMKHCCPMGYKCDPKVQGCTK 718
BLAST of Granulin precursor vs. Ensembl Zebrafish
Match: grna (granulin a [Source:ZFIN;Acc:ZDB-GENE-030131-8434]) HSP 1 Score: 81.2629 bits (199), Expect = 8.008e-19 Identity = 58/171 (33.92%), Postives = 77/171 (45.03%), Query Frame = 1
Query: 100 VLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEP-KNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVE 609
V + + ++D C N C D TCC+TK + CC AVCC D HCCP G CD C + +V + +P + + N + + V ND V CP+ TCC K ++ CCP AVCC D CCP G C++ C S SV VE
Sbjct: 15 VAVTQASSASSDVPC-NDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCED-SSGSVPWVEKVPVRPIRKQKVANTKVSSDVACNDTVACPDG-------STCCKDKDGDWGCCPLPEAVCCEDFIHCCPHGKKCNVAAGSCDDPSGSVPWVE 176
BLAST of Granulin precursor vs. Ensembl Zebrafish
Match: grna (granulin a [Source:ZFIN;Acc:ZDB-GENE-030131-8434]) HSP 1 Score: 82.4185 bits (202), Expect = 8.447e-18 Identity = 61/186 (32.80%), Postives = 80/186 (43.01%), Query Frame = 1
Query: 52 LIFVVKIYADGVMSFDVLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVE 609
+ +V K+ + V + +V++D C N C D TCC+TK + CC AVCC D HCCP+G C+ KC + + K P ++ V ND CP+ TCC TK + CCP AVCC D CCP G CD+ C S SV VE
Sbjct: 349 VSWVEKVPVRPIRKQKVAVTQVSSVSSDVPC-NDTAACADGTTCCKTKEGDWACCPLPEAVCCEDFVHCCPKGKKCNIAAMKCEDPLCTEEPLVKQTPVQSTTTPNVIGTQKSDVPCNDTAACPDG-------STCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDPSGSVPWVE 526 HSP 2 Score: 80.8777 bits (198), Expect = 3.021e-17 Identity = 59/190 (31.05%), Postives = 83/190 (43.68%), Query Frame = 1
Query: 52 LIFVVKIYADGVMSFDVLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNE----NDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVE 609
+ ++ K+ + V + + ++D C N C D TCC+TK + CC AVCC D HCCP G CD C + +V + +P + + N + V+ ND V CP+ TCC K ++ CCP AVCC D CCP G C++ C S SV VE
Sbjct: 610 VPWMDKVPVRPIKKQKVAVTQASSASSDVPC-NDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCED-SSGSVPWVEKVPVRPIRKQKVANTKVSPVSSDVACNDTVACPDG-------STCCKDKDGDWGCCPLPEAVCCEDFIHCCPHGKKCNVAAGSCDDPSGSVPWVE 790 HSP 3 Score: 79.7221 bits (195), Expect = 8.038e-17 Identity = 64/191 (33.51%), Postives = 83/191 (43.46%), Query Frame = 1
Query: 52 LIFVVKIYADGVMSFDVLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAK-----VNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVE 609
+ +V K+ + V + V++D C N C D TCC+ K + CC AVCC D HCCP G C+ C + S M K +P + + K + +A V ND CP+ TCC TK + CCP AVCC D CCP G CD+ C SS SV VE
Sbjct: 522 VPWVEKVPVRPIRKQKVAVTTVSPVSSDIAC-NDTVACPDGSTCCKDKDGEWGCCPLPEAVCCEDFIHCCPHGKKCNVAAGSCDDPSGSVPWMDK-VPVRPIKKQ-KVAVTQASSASSDVPCNDTAACPDG-------STCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDSSGSVPWVE 702 HSP 4 Score: 78.5666 bits (192), Expect = 2.000e-16 Identity = 63/191 (32.98%), Postives = 77/191 (40.31%), Query Frame = 1
Query: 139 ICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPA-LKVDDE------PKNNILEAK---------------------------VNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVE 609
+C N ++C TCCQ + CC AVCC DK HCCPE + CD K KCV + M PA L+ + E P+ A+ V ND CP+ TCC TK + CCP AVCC D CCP G CD+ C S SV+ VE
Sbjct: 170 LCGNGTSECPADTTCCQADDGQWGCCPMPKAVCCDDKIHCCPEDTVCDVKALKCVSSTNQELPMWDKFPARLRAEWEDHKQKRPETQRTTARPTGTTSTTTAANQMTTLPAEHQGVSSDVACNDTAACPDG-------STCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDPSGSVSWVE 353 HSP 5 Score: 75.485 bits (184), Expect = 2.355e-15 Identity = 57/162 (35.19%), Postives = 76/162 (46.91%), Query Frame = 1
Query: 94 FDVLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNE-NDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKC 576
L + V++D C N C D TCC+TK + CC AVCC D HCCP G CD C +V + +P + K + +V+ + +V C N+ C D TCC TK ++ CCP AVCC D CCP+G C+I KC
Sbjct: 275 MTTLPAEHQGVSSDVAC-NDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCED-PSGSVSWVEKVPVRPIR---KQKVAVTQVSSVSSDVPC-NDTAACADGTTCCKTKEGDWACCPLPEAVCCEDFVHCCPKGKKCNIAAMKC 430 HSP 6 Score: 71.633 bits (174), Expect = 4.822e-14 Identity = 46/136 (33.82%), Postives = 69/136 (50.74%), Query Frame = 1
Query: 148 NKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTC 555
++++ C+ TCC + CC + AVCCPD+ HCCPEG CD ++ CVK + VE+ +L ++P+ +++ V C + +TCC+T + CCP AVCC D CCP G C
Sbjct: 890 DEQSSCSADSTCCLLSKDETGCCPFPEAVCCPDQKHCCPEGYRCDLRRRSCVKTTRLYVEITQLTHIRS--NKPQPSVVVKDVQCGGGFSCHDG-------ETCCLTSQTTWGCCPSPKAVCCDDMQHCCPAGYKC 1016 HSP 7 Score: 68.1662 bits (165), Expect = 7.883e-13 Identity = 53/182 (29.12%), Postives = 75/182 (41.21%), Query Frame = 1
Query: 52 LIFVVKIYADGVMSFDVLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSV 597
+ +V K+ + V V++D C N C D TCC+ K + CC AVCC D HCCP G C+ C + S + K+ L+ P KV N CP + T CK+ + CCP AVCC+D CCP C++ + C+K +
Sbjct: 698 VPWVEKVPVRPIRKQKVANTKVSPVSSDVAC-NDTVACPDGSTCCKDKDGDWGCCPLPEAVCCEDFIHCCPHGKKCNVAAGSCDDPSGSVPWVEKVPVHLRAGQRPSG-----KVKCNATHGCPESSTCCKNIA-------GEWGCCPFSQAVCCTDGEHCCPAHYKCNLSSVSCIKGDVVI 866 HSP 8 Score: 65.4698 bits (158), Expect = 6.082e-12 Identity = 49/165 (29.70%), Postives = 67/165 (40.61%), Query Frame = 1
Query: 142 CPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKN--NILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVELPNDAEE 630
CPN C Q+CCQ + CC + + CC D HCCPEG C K C + + + K D PK+ I +E+D + CP+ + C+ +Y CCP + CSD CCP C + C+K V V N E
Sbjct: 19 CPNNEV-CEAGQSCCQDPTGGFSCCPFHHGECCEDHLHCCPEGMLCSVKDLTCTNATHTQPLADRTL--AKKPDLPKSFRMIFSMPASESD-ITCPDG--SSCPAEFSCLLMSTSYGCCPVAQGLACSDGKHCCPNDHECSTDSSLCVKRKVKVQTVLCGNGTSE 177 HSP 9 Score: 63.929 bits (154), Expect = 1.743e-11 Identity = 48/152 (31.58%), Postives = 69/152 (45.39%), Query Frame = 1
Query: 148 NKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIP-----ALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSS 588
N C + TCC+ + CC ++ AVCC D HCCP C+ V C K +V +IP A + PK ++ K +E TCC+ + CCP AVCC D+ CCPEG CD++ + C+K++
Sbjct: 809 NATHGCPESSTCCKNIAGEWGCCPFSQAVCCTDGEHCCPAHYKCNLSS---VSCIKGDV----VIPWYNKIAAESTPAPKLDLGVVKCDEQSSC---------SADSTCCLLSKDETGCCPFPEAVCCPDQKHCCPEGYRCDLRRRSCVKTT 944 HSP 10 Score: 57.7658 bits (138), Expect = 2.164e-9 Identity = 52/156 (33.33%), Postives = 62/156 (39.74%), Query Frame = 1
Query: 163 CTDRQTC-----CQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVELP 615
C D +C C SY CC A + C D HCCP C CVK + KV V+C N ++C TCC + CCP AVCC DK CCPE T CD+K KC+ S+ N ELP
Sbjct: 109 CPDGSSCPAEFSCLLMSTSYGCCPVAQGLACSDGKHCCPNDHECSTDSSLCVK-------------------------RKVKVQT---VLCGNGTSECPADTTCCQADDGQWGCCPMPKAVCCDDKIHCCPEDTVCDVKALKCVSST----NQELP 232
BLAST of Granulin precursor vs. Ensembl Zebrafish
Match: grna (granulin a [Source:ZFIN;Acc:ZDB-GENE-030131-8434]) HSP 1 Score: 82.4185 bits (202), Expect = 8.890e-18 Identity = 61/186 (32.80%), Postives = 80/186 (43.01%), Query Frame = 1
Query: 52 LIFVVKIYADGVMSFDVLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVE 609
+ +V K+ + V + +V++D C N C D TCC+TK + CC AVCC D HCCP+G C+ KC + + K P ++ V ND CP+ TCC TK + CCP AVCC D CCP G CD+ C S SV VE
Sbjct: 349 VSWVEKVPVRPIRKQKVAVTQVSSVSSDVPC-NDTAACADGTTCCKTKEGDWACCPLPEAVCCEDFVHCCPKGKKCNIAAMKCEDPLCTEEPLVKQTPVQSTTTPNVIGTQKSDVPCNDTAACPDG-------STCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDPSGSVPWVE 526 HSP 2 Score: 78.5666 bits (192), Expect = 1.826e-16 Identity = 63/191 (32.98%), Postives = 77/191 (40.31%), Query Frame = 1
Query: 139 ICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPA-LKVDDE------PKNNILEAK---------------------------VNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVE 609
+C N ++C TCCQ + CC AVCC DK HCCPE + CD K KCV + M PA L+ + E P+ A+ V ND CP+ TCC TK + CCP AVCC D CCP G CD+ C S SV+ VE
Sbjct: 170 LCGNGTSECPADTTCCQADDGQWGCCPMPKAVCCDDKIHCCPEDTVCDVKALKCVSSTNQELPMWDKFPARLRAEWEDHKQKRPETQRTTARPTGTTSTTTAANQMTTLPAEHQGVSSDVACNDTAACPDG-------STCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDPSGSVSWVE 353 HSP 3 Score: 75.485 bits (184), Expect = 2.551e-15 Identity = 57/162 (35.19%), Postives = 76/162 (46.91%), Query Frame = 1
Query: 94 FDVLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNE-NDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKC 576
L + V++D C N C D TCC+TK + CC AVCC D HCCP G CD C +V + +P + K + +V+ + +V C N+ C D TCC TK ++ CCP AVCC D CCP+G C+I KC
Sbjct: 275 MTTLPAEHQGVSSDVAC-NDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCED-PSGSVSWVEKVPVRPIR---KQKVAVTQVSSVSSDVPC-NDTAACADGTTCCKTKEGDWACCPLPEAVCCEDFVHCCPKGKKCNIAAMKC 430 HSP 4 Score: 75.0998 bits (183), Expect = 2.913e-15 Identity = 54/158 (34.18%), Postives = 67/158 (42.41%), Query Frame = 1
Query: 148 NKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNE----NDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVE 609
N C D TCC+TK + CC AVCC D HCCP G CD C +V + +P + + + V+ ND V CP+ T CKD + CCP AVCC D CCP G C++ C S SV VE
Sbjct: 465 NDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCED-PSGSVPWVEKVPVRPIRKQKVAVTTVSPVSSDIACNDTVACPDGSTCCKDK-------DGEWGCCPLPEAVCCEDFIHCCPHGKKCNVAAGSCDDPSGSVPWVE 614 HSP 5 Score: 71.633 bits (174), Expect = 4.163e-14 Identity = 46/136 (33.82%), Postives = 69/136 (50.74%), Query Frame = 1
Query: 148 NKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTC 555
++++ C+ TCC + CC + AVCCPD+ HCCPEG CD ++ CVK + VE+ +L ++P+ +++ V C + +TCC+T + CCP AVCC D CCP G C
Sbjct: 714 DEQSSCSADSTCCLLSKDETGCCPFPEAVCCPDQKHCCPEGYRCDLRRRSCVKTTRLYVEITQLTHIRS--NKPQPSVVVKDVQCGGGFSCHDG-------ETCCLTSQTTWGCCPSPKAVCCDDMQHCCPAGYKC 840 HSP 6 Score: 70.4774 bits (171), Expect = 1.092e-13 Identity = 53/182 (29.12%), Postives = 76/182 (41.76%), Query Frame = 1
Query: 52 LIFVVKIYADGVMSFDVLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSV 597
+ +V K+ + V + V++D C N C D TCC+ K + CC AVCC D HCCP G C+ C + S + K+ L+ P KV N CP + T CK+ + CCP AVCC+D CCP C++ + C+K +
Sbjct: 522 VPWVEKVPVRPIRKQKVAVTTVSPVSSDIAC-NDTVACPDGSTCCKDKDGEWGCCPLPEAVCCEDFIHCCPHGKKCNVAAGSCDDPSGSVPWVEKVPVHLRAGQRPSG-----KVKCNATHGCPESSTCCKNIA-------GEWGCCPFSQAVCCTDGEHCCPAHYKCNLSSVSCIKGDVVI 690 HSP 7 Score: 65.855 bits (159), Expect = 4.970e-12 Identity = 48/165 (29.09%), Postives = 68/165 (41.21%), Query Frame = 1
Query: 142 CPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKN--NILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVELPNDAEE 630
CPN C Q+CCQ + CC + + CC D HCCPEG C K C + + + K D PK+ I +E+D + CP+ + + ++ +Y CCP + CSD CCP C + C+K V V N E
Sbjct: 19 CPNNEV-CEAGQSCCQDPTGGFSCCPFHHGECCEDHLHCCPEGMLCSVKDLTCTNATHTQPLADRTL--AKKPDLPKSFRMIFSMPASESD-ITCPDGSSCPAEFSCLLMS--TSYGCCPVAQGLACSDGKHCCPNDHECSTDSSLCVKRKVKVQTVLCGNGTSE 177 HSP 8 Score: 64.3142 bits (155), Expect = 1.493e-11 Identity = 48/152 (31.58%), Postives = 69/152 (45.39%), Query Frame = 1
Query: 148 NKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIP-----ALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSS 588
N C + TCC+ + CC ++ AVCC D HCCP C+ V C K +V +IP A + PK ++ K +E TCC+ + CCP AVCC D+ CCPEG CD++ + C+K++
Sbjct: 633 NATHGCPESSTCCKNIAGEWGCCPFSQAVCCTDGEHCCPAHYKCNLSS---VSCIKGDV----VIPWYNKIAAESTPAPKLDLGVVKCDEQSSC---------SADSTCCLLSKDETGCCPFPEAVCCPDQKHCCPEGYRCDLRRRSCVKTT 768 HSP 9 Score: 57.7658 bits (138), Expect = 2.219e-9 Identity = 49/144 (34.03%), Postives = 58/144 (40.28%), Query Frame = 1
Query: 184 CQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVELP 615
C SY CC A + C D HCCP C CVK + KV V+C N ++C TCC + CCP AVCC DK CCPE T CD+K KC+ S+ N ELP
Sbjct: 121 CLLMSTSYGCCPVAQGLACSDGKHCCPNDHECSTDSSLCVK-------------------------RKVKVQT---VLCGNGTSECPADTTCCQADDGQWGCCPMPKAVCCDDKIHCCPEDTVCDVKALKCVSST----NQELP 232
BLAST of Granulin precursor vs. Ensembl Zebrafish
Match: grna (granulin a [Source:ZFIN;Acc:ZDB-GENE-030131-8434]) HSP 1 Score: 82.4185 bits (202), Expect = 9.323e-18 Identity = 61/186 (32.80%), Postives = 80/186 (43.01%), Query Frame = 1
Query: 52 LIFVVKIYADGVMSFDVLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVE 609
+ +V K+ + V + +V++D C N C D TCC+TK + CC AVCC D HCCP+G C+ KC + + K P ++ V ND CP+ TCC TK + CCP AVCC D CCP G CD+ C S SV VE
Sbjct: 349 VSWVEKVPVRPIRKQKVAVTQVSSVSSDVPC-NDTAACADGTTCCKTKEGDWACCPLPEAVCCEDFVHCCPKGKKCNIAAMKCEDPLCTEEPLVKQTPVQSTTTPNVIGTQKSDVPCNDTAACPDG-------STCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDPSGSVPWVE 526 HSP 2 Score: 78.5666 bits (192), Expect = 1.933e-16 Identity = 63/191 (32.98%), Postives = 77/191 (40.31%), Query Frame = 1
Query: 139 ICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPA-LKVDDE------PKNNILEAK---------------------------VNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVE 609
+C N ++C TCCQ + CC AVCC DK HCCPE + CD K KCV + M PA L+ + E P+ A+ V ND CP+ TCC TK + CCP AVCC D CCP G CD+ C S SV+ VE
Sbjct: 170 LCGNGTSECPADTTCCQADDGQWGCCPMPKAVCCDDKIHCCPEDTVCDVKALKCVSSTNQELPMWDKFPARLRAEWEDHKQKRPETQRTTARPTGTTSTTTAANQMTTLPAEHQGVSSDVACNDTAACPDG-------STCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDPSGSVSWVE 353 HSP 3 Score: 75.0998 bits (183), Expect = 2.700e-15 Identity = 57/162 (35.19%), Postives = 76/162 (46.91%), Query Frame = 1
Query: 94 FDVLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNE-NDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKC 576
L + V++D C N C D TCC+TK + CC AVCC D HCCP G CD C +V + +P + K + +V+ + +V C N+ C D TCC TK ++ CCP AVCC D CCP+G C+I KC
Sbjct: 275 MTTLPAEHQGVSSDVAC-NDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCED-PSGSVSWVEKVPVRPIR---KQKVAVTQVSSVSSDVPC-NDTAACADGTTCCKTKEGDWACCPLPEAVCCEDFVHCCPKGKKCNIAAMKC 430 HSP 4 Score: 73.9442 bits (180), Expect = 7.164e-15 Identity = 52/158 (32.91%), Postives = 65/158 (41.14%), Query Frame = 1
Query: 148 NKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDE----PKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVE 609
N C D TCC+TK + CC AVCC D HCCP G CD C +V + +P + + + + V ND CP+ T CK + CCP AVCC D CCP G C++ C S SV VE
Sbjct: 465 NDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCED-PSGSVPWVEKVPVRPIRKQKVAVTTASSASSDVPCNDTAACPDGSTCCKTK-------DGGWACCPLPEAVCCEDFIHCCPHGKKCNVAAGSCDDPSGSVPWVE 614 HSP 5 Score: 72.4034 bits (176), Expect = 2.230e-14 Identity = 53/182 (29.12%), Postives = 77/182 (42.31%), Query Frame = 1
Query: 52 LIFVVKIYADGVMSFDVLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSV 597
+ +V K+ + V + + ++D C N C D TCC+TK + CC AVCC D HCCP G C+ C + S + K+ L+ P KV N CP + T CK+ + CCP AVCC+D CCP C++ + C+K +
Sbjct: 522 VPWVEKVPVRPIRKQKVAVTTASSASSDVPC-NDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCNVAAGSCDDPSGSVPWVEKVPVHLRAGQRPSG-----KVKCNATHGCPESSTCCKNIA-------GEWGCCPFSQAVCCTDGEHCCPAHYKCNLSSVSCIKGDVVI 690 HSP 6 Score: 71.633 bits (174), Expect = 4.490e-14 Identity = 46/136 (33.82%), Postives = 69/136 (50.74%), Query Frame = 1
Query: 148 NKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTC 555
++++ C+ TCC + CC + AVCCPD+ HCCPEG CD ++ CVK + VE+ +L ++P+ +++ V C + +TCC+T + CCP AVCC D CCP G C
Sbjct: 714 DEQSSCSADSTCCLLSKDETGCCPFPEAVCCPDQKHCCPEGYRCDLRRRSCVKTTRLYVEITQLTHIRS--NKPQPSVVVKDVQCGGGFSCHDG-------ETCCLTSQTTWGCCPSPKAVCCDDMQHCCPAGYKC 840 HSP 7 Score: 65.4698 bits (158), Expect = 5.358e-12 Identity = 48/165 (29.09%), Postives = 68/165 (41.21%), Query Frame = 1
Query: 142 CPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKN--NILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVELPNDAEE 630
CPN C Q+CCQ + CC + + CC D HCCPEG C K C + + + K D PK+ I +E+D + CP+ + + ++ +Y CCP + CSD CCP C + C+K V V N E
Sbjct: 19 CPNNEV-CEAGQSCCQDPTGGFSCCPFHHGECCEDHLHCCPEGMLCSVKDLTCTNATHTQPLADRTL--AKKPDLPKSFRMIFSMPASESD-ITCPDGSSCPAEFSCLLMS--TSYGCCPVAQGLACSDGKHCCPNDHECSTDSSLCVKRKVKVQTVLCGNGTSE 177 HSP 8 Score: 64.3142 bits (155), Expect = 1.625e-11 Identity = 48/152 (31.58%), Postives = 69/152 (45.39%), Query Frame = 1
Query: 148 NKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIP-----ALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSS 588
N C + TCC+ + CC ++ AVCC D HCCP C+ V C K +V +IP A + PK ++ K +E TCC+ + CCP AVCC D+ CCPEG CD++ + C+K++
Sbjct: 633 NATHGCPESSTCCKNIAGEWGCCPFSQAVCCTDGEHCCPAHYKCNLSS---VSCIKGDV----VIPWYNKIAAESTPAPKLDLGVVKCDEQSSC---------SADSTCCLLSKDETGCCPFPEAVCCPDQKHCCPEGYRCDLRRRSCVKTT 768 HSP 9 Score: 57.7658 bits (138), Expect = 2.240e-9 Identity = 49/144 (34.03%), Postives = 58/144 (40.28%), Query Frame = 1
Query: 184 CQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVELP 615
C SY CC A + C D HCCP C CVK + KV V+C N ++C TCC + CCP AVCC DK CCPE T CD+K KC+ S+ N ELP
Sbjct: 121 CLLMSTSYGCCPVAQGLACSDGKHCCPNDHECSTDSSLCVK-------------------------RKVKVQT---VLCGNGTSECPADTTCCQADDGQWGCCPMPKAVCCDDKIHCCPEDTVCDVKALKCVSST----NQELP 232
BLAST of Granulin precursor vs. Ensembl Xenopus
Match: trappc11 (trafficking protein particle complex 11 [Source:Xenbase;Acc:XB-GENE-5931148]) HSP 1 Score: 87.8113 bits (216), Expect = 1.739e-19 Identity = 62/183 (33.88%), Postives = 90/183 (49.18%), Query Frame = 1
Query: 100 VLLENQKNVTTDNICPNKRTKCTDRQTCCQ-TKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNV-ELPNDAEENGNK 642
+ NQ V +CP+ R++C TCC + +S+ CC AVCC D HCCP S CD +Q +C+ M KL +K + + E +V D CP+ T C+ + Y CC +AVCCSD CCP GTTCD+ +KKC+ + + ++P EE+ N+
Sbjct: 131 IPASNQSAV----VCPDGRSECPTLTTCCMMSDMSSWGCCPMPQAVCCDDHMHCCPHNSECDVQQGRCISNQDHIPWMSKLPARVKSEGDE-----ERRVPCLDGTFCPDGSTCCEQVD-------HTYGCCSILSAVCCSDHLHCCPGGTTCDLVHKKCVSQTGEGPLLPQMPAIREESANQ 297 HSP 2 Score: 77.7962 bits (190), Expect = 4.859e-16 Identity = 51/147 (34.69%), Postives = 63/147 (42.86%), Query Frame = 1
Query: 157 TKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSV 597
T C D TCC+ ++Y CC +AVCC D HCCP G+TCD KCV S G L+P + E N + E C + CCP AVCC+D CCPEG TC +C K S+
Sbjct: 227 TFCPDGSTCCEQVDHTYGCCSILSAVCCSDHLHCCPGGTTCDLVHKKCV----SQTGEGPLLPQMPAIREESANQVPCDATTKKETCC--------------RLVSGKWGCCPVVKAVCCNDHLHCCPEGYTC--SQGECSKMEHSI 353 HSP 3 Score: 74.7146 bits (182), Expect = 6.408e-15 Identity = 53/162 (32.72%), Postives = 72/162 (44.44%), Query Frame = 1
Query: 160 KCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNV----ELPNDAEEN 633
C D QTCC+ + CC A AVCC D HCCP G TC Q + ++ PALK + V +D C + +T C+ ++ CCP AVCCSD + CCP+G TCD + C+ FS+ + LP D +
Sbjct: 450 SCADGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCSGSQ---CMIGEHSIPWFSKTPALKQKAK--------DVQCDDMYSCADGQTCCRLAS-------GDWGCCPIAQAVCCSDHWHCCPQGFTCDARG-TCVLGQFSIPWLTKVPALPFDGTHS 592 HSP 4 Score: 63.5438 bits (153), Expect = 3.599e-11 Identity = 54/166 (32.53%), Postives = 73/166 (43.98%), Query Frame = 1
Query: 100 VLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSV 597
L ++V D++ C D QTCC+ + CC A AVCC D HCCP G TC Q C K + ++ + PAL+ E V C ++ C D +TCC ++ CCP AVCC D CCP G TC +C+ S+
Sbjct: 361 ALTHEARDVECDDM-----YSCPDGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCSGGQ--CQK-GEHSIPLFSKTPALR--------------QETKYVKC-DDTHSCADGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTC--SGSQCMIGEHSI 501 HSP 5 Score: 58.9214 bits (141), Expect = 1.513e-9 Identity = 33/87 (37.93%), Postives = 46/87 (52.87%), Query Frame = 1
Query: 100 VLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVD 360
L + K+V D++ C D QTCC+ + CC A AVCC D +HCCP+G TCDA+ CV + ++ +PAL D
Sbjct: 509 ALKQKAKDVQCDDM-----YSCADGQTCCRLASGDWGCCPIAQAVCCSDHWHCCPQGFTCDARG-TCVL-GQFSIPWLTKVPALPFD 588 HSP 6 Score: 51.6026 bits (122), Expect = 4.361e-7 Identity = 32/135 (23.70%), Postives = 52/135 (38.52%), Query Frame = 1
Query: 7 SKM-KNFVAILGFNLLLIFVVKIYADGVMSFDVLLENQ----------KNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNN 378
SKM K V +L ++ V + ++ + L + + + D + + + C D QTCC+ ++CC Y VCCPD HCCP G C C + + P P++
Sbjct: 623 SKMVKRTVILLATACIVTLVSFSFCPDIIIYYALQKEAIETSPSDTSVQGLRLDYVWCDSQYACFDGQTCCRGLGGVWNCCVYTQGVCCPDMVHCCPYGYVCLNHGTSCSRSGSPRWDGQSGSPHWDGKQSPRDG 757
BLAST of Granulin precursor vs. Ensembl Xenopus
Match: trappc11 (trafficking protein particle complex 11 [Source:Xenbase;Acc:XB-GENE-5931148]) HSP 1 Score: 87.4261 bits (215), Expect = 2.234e-19 Identity = 62/183 (33.88%), Postives = 90/183 (49.18%), Query Frame = 1
Query: 100 VLLENQKNVTTDNICPNKRTKCTDRQTCCQ-TKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNV-ELPNDAEENGNK 642
+ NQ V +CP+ R++C TCC + +S+ CC AVCC D HCCP S CD +Q +C+ M KL +K + + E +V D CP+ T C+ + Y CC +AVCCSD CCP GTTCD+ +KKC+ + + ++P EE+ N+
Sbjct: 111 IPASNQSAV----VCPDGRSECPTLTTCCMMSDMSSWGCCPMPQAVCCDDHMHCCPHNSECDVQQGRCISNQDHIPWMSKLPARVKSEGDE-----ERRVPCLDGTFCPDGSTCCEQVD-------HTYGCCSILSAVCCSDHLHCCPGGTTCDLVHKKCVSQTGEGPLLPQMPAIREESANQ 277 HSP 2 Score: 77.7962 bits (190), Expect = 5.727e-16 Identity = 51/147 (34.69%), Postives = 63/147 (42.86%), Query Frame = 1
Query: 157 TKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSV 597
T C D TCC+ ++Y CC +AVCC D HCCP G+TCD KCV S G L+P + E N + E C + CCP AVCC+D CCPEG TC +C K S+
Sbjct: 207 TFCPDGSTCCEQVDHTYGCCSILSAVCCSDHLHCCPGGTTCDLVHKKCV----SQTGEGPLLPQMPAIREESANQVPCDATTKKETCC--------------RLVSGKWGCCPVVKAVCCNDHLHCCPEGYTC--SQGECSKMEHSI 333 HSP 3 Score: 74.3294 bits (181), Expect = 7.694e-15 Identity = 53/162 (32.72%), Postives = 72/162 (44.44%), Query Frame = 1
Query: 160 KCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNV----ELPNDAEEN 633
C D QTCC+ + CC A AVCC D HCCP G TC Q + ++ PALK + V +D C + +T C+ ++ CCP AVCCSD + CCP+G TCD + C+ FS+ + LP D +
Sbjct: 430 SCADGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCSGSQ---CMIGEHSIPWFSKTPALKQKAK--------DVQCDDMYSCADGQTCCRLAS-------GDWGCCPIAQAVCCSDHWHCCPQGFTCDARG-TCVLGQFSIPWLTKVPALPFDGTHS 572 HSP 4 Score: 63.5438 bits (153), Expect = 4.004e-11 Identity = 54/166 (32.53%), Postives = 73/166 (43.98%), Query Frame = 1
Query: 100 VLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSV 597
L ++V D++ C D QTCC+ + CC A AVCC D HCCP G TC Q C K + ++ + PAL+ E V C ++ C D +TCC ++ CCP AVCC D CCP G TC +C+ S+
Sbjct: 341 ALTHEARDVECDDM-----YSCPDGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCSGGQ--CQK-GEHSIPLFSKTPALR--------------QETKYVKC-DDTHSCADGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTC--SGSQCMIGEHSI 481 HSP 5 Score: 58.5362 bits (140), Expect = 1.698e-9 Identity = 33/87 (37.93%), Postives = 46/87 (52.87%), Query Frame = 1
Query: 100 VLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVD 360
L + K+V D++ C D QTCC+ + CC A AVCC D +HCCP+G TCDA+ CV + ++ +PAL D
Sbjct: 489 ALKQKAKDVQCDDM-----YSCADGQTCCRLASGDWGCCPIAQAVCCSDHWHCCPQGFTCDARG-TCVL-GQFSIPWLTKVPALPFD 568 HSP 6 Score: 51.6026 bits (122), Expect = 4.296e-7 Identity = 32/135 (23.70%), Postives = 52/135 (38.52%), Query Frame = 1
Query: 7 SKM-KNFVAILGFNLLLIFVVKIYADGVMSFDVLLENQ----------KNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNN 378
SKM K V +L ++ V + ++ + L + + + D + + + C D QTCC+ ++CC Y VCCPD HCCP G C C + + P P++
Sbjct: 603 SKMVKRTVILLATACIVTLVSFSFCPDIIIYYALQKEAIETSPSDTSVQGLRLDYVWCDSQYACFDGQTCCRGLGGVWNCCVYTQGVCCPDMVHCCPYGYVCLNHGTSCSRSGSPRWDGQSGSPHWDGKQSPRDG 737
BLAST of Granulin precursor vs. Ensembl Xenopus
Match: trappc11 (trafficking protein particle complex 11 [Source:Xenbase;Acc:XB-GENE-5931148]) HSP 1 Score: 87.0409 bits (214), Expect = 4.062e-19 Identity = 62/185 (33.51%), Postives = 90/185 (48.65%), Query Frame = 1
Query: 100 VLLENQKNVTTDNICPNKRTKCTDRQTCCQ-TKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNIL--EAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNV-ELPNDAEENGNK 642
+ NQ V +CP+ R++C TCC + +S+ CC AVCC D HCCP S CD +Q +C+ M KL +K + + E +V D CP+ T C+ + Y CC +AVCCSD CCP GTTCD+ +KKC+ + + ++P EE+ N+
Sbjct: 111 IPASNQSAV----VCPDGRSECPTLTTCCMMSDMSSWGCCPMPQAVCCDDHMHCCPHNSECDVQQGRCISNQDHIPWMSKLPARVKSEGLKLVGLGDEERRVPCLDGTFCPDGSTCCEQVD-------HTYGCCSILSAVCCSDHLHCCPGGTTCDLVHKKCVSQTGEGPLLPQMPAIREESANQ 284 HSP 2 Score: 75.8702 bits (185), Expect = 2.092e-15 Identity = 52/147 (35.37%), Postives = 68/147 (46.26%), Query Frame = 1
Query: 157 TKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSV 597
T C D TCC+ ++Y CC +AVCC D HCCP G+TCD KCV S G L+P + E N +V + CP+ T C + + CCP+ AVCC D CCP G TC C+ + S+
Sbjct: 214 TFCPDGSTCCEQVDHTYGCCSILSAVCCSDHLHCCPGGTTCDLVHKKCV----SQTGEGPLLPQMPAIREESAN----QVPCDATTSCPDKNTCCHLSS-------EKWGCCPYAQAVCCDDHIHCCPSGFTC--SGGSCVLAEHSI 343 HSP 3 Score: 63.5438 bits (153), Expect = 3.656e-11 Identity = 50/160 (31.25%), Postives = 66/160 (41.25%), Query Frame = 1
Query: 160 KCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVELPNDAEENGN 639
C D QTCC+ + CC A AVCC D HCCP G TC Q + ++ PALK + V +D C + +T C+ ++ CCP AVCC D CCP G TC +C K S+ ++ GN
Sbjct: 514 SCADGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCSGSQ---CMIGEHSIPWFSKTPALKQKAK--------DVQCDDMYSCADGQTCCRLAS-------GDWGCCPIAQAVCCDDHEHCCPPGYTC--SGGQCQKGEHSIPWYSKTPALKQEGN 653 HSP 4 Score: 63.1586 bits (152), Expect = 5.079e-11 Identity = 54/166 (32.53%), Postives = 73/166 (43.98%), Query Frame = 1
Query: 100 VLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSV 597
L ++V D++ C D QTCC+ + CC A AVCC D HCCP G TC Q C K + ++ + PAL+ E V C ++ C D +TCC ++ CCP AVCC D CCP G TC +C+ S+
Sbjct: 425 ALTHEARDVECDDM-----YSCPDGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCSGGQ--CQK-GEHSIPLFSKTPALR--------------QETKYVKC-DDTHSCADGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTC--SGSQCMIGEHSI 565 HSP 5 Score: 62.7734 bits (151), Expect = 6.922e-11 Identity = 51/161 (31.68%), Postives = 74/161 (45.96%), Query Frame = 1
Query: 118 KNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVM-CPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSV 597
+ + T + + C +++TCC+ + CC AVCC D HCCPEG TC Q +C K + ++ PAL EA+ E D++ CP+ +T C+ ++ CCP AVCC D CCP G TC +C K S+
Sbjct: 352 QGLKTTRVQCDDTASCPEKETCCRLVSGKWGCCPVVKAVCCNDHLHCCPEGYTC--SQGECSK-MEHSIPWFTKTPAL---------THEARDVECDDMYSCPDGQTCCRLAS-------GDWGCCPIAQAVCCDDHEHCCPPGYTC--SGGQCQKGEHSI 491 HSP 6 Score: 51.2174 bits (121), Expect = 4.931e-7 Identity = 32/135 (23.70%), Postives = 52/135 (38.52%), Query Frame = 1
Query: 7 SKM-KNFVAILGFNLLLIFVVKIYADGVMSFDVLLENQ----------KNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNN 378
SKM K V +L ++ V + ++ + L + + + D + + + C D QTCC+ ++CC Y VCCPD HCCP G C C + + P P++
Sbjct: 808 SKMVKRTVILLATACIVTLVSFSFCPDIIIYYALQKEAIETSPSDTSVQGLRLDYVWCDSQYACFDGQTCCRGLGGVWNCCVYTQGVCCPDMVHCCPYGYVCLNHGTSCSRSGSPRWDGQSGSPHWDGKQSPRDG 942
BLAST of Granulin precursor vs. Ensembl Xenopus
Match: trappc11 (trafficking protein particle complex 11 [Source:Xenbase;Acc:XB-GENE-5931148]) HSP 1 Score: 86.6557 bits (213), Expect = 5.126e-19 Identity = 62/185 (33.51%), Postives = 90/185 (48.65%), Query Frame = 1
Query: 100 VLLENQKNVTTDNICPNKRTKCTDRQTCCQ-TKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNIL--EAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNV-ELPNDAEENGNK 642
+ NQ V +CP+ R++C TCC + +S+ CC AVCC D HCCP S CD +Q +C+ M KL +K + + E +V D CP+ T C+ + Y CC +AVCCSD CCP GTTCD+ +KKC+ + + ++P EE+ N+
Sbjct: 111 IPASNQSAV----VCPDGRSECPTLTTCCMMSDMSSWGCCPMPQAVCCDDHMHCCPHNSECDVQQGRCISNQDHIPWMSKLPARVKSEGLKLVGLGDEERRVPCLDGTFCPDGSTCCEQVD-------HTYGCCSILSAVCCSDHLHCCPGGTTCDLVHKKCVSQTGEGPLLPQMPAIREESANQ 284 HSP 2 Score: 75.8702 bits (185), Expect = 2.765e-15 Identity = 52/147 (35.37%), Postives = 68/147 (46.26%), Query Frame = 1
Query: 157 TKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSV 597
T C D TCC+ ++Y CC +AVCC D HCCP G+TCD KCV S G L+P + E N +V + CP+ T C + + CCP+ AVCC D CCP G TC C+ + S+
Sbjct: 214 TFCPDGSTCCEQVDHTYGCCSILSAVCCSDHLHCCPGGTTCDLVHKKCV----SQTGEGPLLPQMPAIREESAN----QVPCDATTSCPDKNTCCHLSS-------EKWGCCPYAQAVCCDDHIHCCPSGFTC--SGGSCVLAEHSI 343 HSP 3 Score: 62.3882 bits (150), Expect = 9.124e-11 Identity = 48/146 (32.88%), Postives = 62/146 (42.47%), Query Frame = 1
Query: 160 KCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSV 597
C D QTCC+ + CC A AVCC D HCCP G TC Q + ++ PALK + V +D C + +T C+ ++ CCP AVCC D CCP G TC +C K S+
Sbjct: 556 SCADGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCSGSQ---CMIGEHSIPWFSKTPALKQKAK--------DVQCDDMYSCADGQTCCRLAS-------GDWGCCPIAQAVCCDDHEHCCPPGYTC--SGGQCQKGEHSI 681 HSP 4 Score: 58.9214 bits (141), Expect = 1.220e-9 Identity = 49/165 (29.70%), Postives = 71/165 (43.03%), Query Frame = 1
Query: 118 KNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKS-----NVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSV 597
+ + T + + C +++TCC+ + CC AVCC D HCCPEG TC Q +C K S M + + P ++ V +D CP+ +T C+ ++ CCP AVCC D CCP G TC +C K S+
Sbjct: 352 QGLKTTRVQCDDTASCPEKETCCRLVSGKWGCCPVVKAVCCNDHLHCCPEGYTC--SQGECSKMEHSIPWFTKTPMVFFLSLSLLSILPVVSVAR-DVECDDMYSCPDGQTCCRLAS-------GDWGCCPIAQAVCCDDHEHCCPPGYTC--SGGQCQKGEHSI 504 HSP 5 Score: 58.9214 bits (141), Expect = 1.327e-9 Identity = 61/212 (28.77%), Postives = 90/212 (42.45%), Query Frame = 1
Query: 7 SKMKNFVAILGFNLLLIFVVKIYADGVMSFDVLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALK---------VDDE---PKNNILEAKV---NENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSV 597
SKM++ + ++ F+ + V ++V D++ C D QTCC+ + CC A AVCC D HCCP G TC Q C K + ++ + PAL+ DDE P + + + V C ++ C D +TCC ++ CCP AVCC D CCP G TC +C+ S+
Sbjct: 411 SKMEHSIPWFTKTPMVFFLSLSLLSILPVVSV----ARDVECDDM-----YSCPDGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCSGGQ--CQK-GEHSIPLFSKTPALRQETACARRCGDDEYPFPLCLLFSPFICPFSVAKYVKC-DDTHSCADGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTC--SGSQCMIGEHSI 607 HSP 6 Score: 51.2174 bits (121), Expect = 4.686e-7 Identity = 32/87 (36.78%), Postives = 42/87 (48.28%), Query Frame = 1
Query: 103 LLENQKNVTTDNICP-NKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVD 360
L NV NI + C D TCC+ + CC AVCC D +HCCP+G TCDA+ CV + ++ +PAL D
Sbjct: 698 LANTPVNVFAGNIVKCDDSFACKDGYTCCRMVSGVWGCCPIEKAVCCSDHWHCCPQGFTCDARG-TCVL-GQFSIPWLTKVPALPFD 782 HSP 7 Score: 48.9062 bits (115), Expect = 3.079e-6 Identity = 24/87 (27.59%), Postives = 36/87 (41.38%), Query Frame = 1
Query: 118 KNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNN 378
+ + D + + + C D QTCC+ ++CC Y VCCPD HCCP G C C + + P P++
Sbjct: 829 QGLRLDYVWCDSQYACFDGQTCCRGLGGVWNCCVYTQGVCCPDMVHCCPYGYVCLNHGTSCSRSGSPRWDGQSGSPHWDGKQSPRDG 915
BLAST of Granulin precursor vs. Ensembl Mouse
Match: Grn (granulin [Source:MGI Symbol;Acc:MGI:95832]) HSP 1 Score: 87.4261 bits (215), Expect = 1.899e-19 Identity = 59/160 (36.88%), Postives = 83/160 (51.88%), Query Frame = 1
Query: 142 CPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNV-ELPN 618
CP + +C D TCC S+ CC A CC D+ HCCP G++CD +CV ++ + K PA K + V+ V+CP+ KT+C D TCC Y CCP NA+CCSD CCP+ T CD+ KCL +++ + + +LP
Sbjct: 138 CPGSQFECPDSATCCIMVDGSWGCCPMPQASCCEDRVHCCPHGASCDLVHTRCVSPTGTHTLLKKF-PAQKTNR---------AVSLPFSVVCPDAKTQCPDDSTCCELPTGKYGCCPMPNAICCSDHLHCCPQDTVCDLIQSKCLSKNYTTDLLTKLPG 287 HSP 2 Score: 76.2554 bits (186), Expect = 1.299e-15 Identity = 51/153 (33.33%), Postives = 67/153 (43.79%), Query Frame = 1
Query: 139 ICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSV 597
+CP+ +T+C D TCC+ Y CC NA+CC D HCCP+ + CD Q KC+ + + KL P + E K + EV CP T C+ + CCP AVCC D CCP G C + C V
Sbjct: 219 VCPDAKTQCPDDSTCCELPTGKYGCCPMPNAICCSDHLHCCPQDTVCDLIQSKCLSKNYTTDLLTKL---------PGYPVKEVKCDM--EVSCPEGYTCCRLNT-------GAWGCCPFAKAVCCEDHIHCCPAGFQCHTEKGTCEMGILQV 353 HSP 3 Score: 69.707 bits (169), Expect = 2.324e-13 Identity = 45/158 (28.48%), Postives = 70/158 (44.30%), Query Frame = 1
Query: 148 NKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILE---AKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVEL 612
++ T C QTCC + S+ CC+ +AVCC D+ HCCP G TC+ K C K ++ + P + + PK +E +++ C ++ + CCP+ VCC D CCP G C + KCL+ ++ L
Sbjct: 456 DQHTSCPVGQTCCPSLKGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARTCEK------DVDFIQPPVLLTLGPKVGNVECGEGHFCHDNQTCCKDSA--------------GVWACCPYLKGVCCRDGRHCCPGGFHCSARGTKCLRKKIPRWDMFL 593 HSP 4 Score: 67.0106 bits (162), Expect = 1.608e-12 Identity = 53/182 (29.12%), Postives = 81/182 (44.51%), Query Frame = 1
Query: 100 VLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKL--IPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVELPNDAEENGN 639
+ L + + + +D C + T+C TCC+ + CC AVCC D HCCP+G TC A+ + C K + + L IPA + + + + CP +T C K ++ CC +AVCC D+ CCP G TC++K + C K + L + GN
Sbjct: 363 LRLPDPQILKSDTPC-DDFTRCPTNNTCCKLNSGDWGCCPIPEAVCCSDNQHCCPQGFTCLAQGY----CQKGDTMVAGLEKIPARQT-----TPLQIGDIGCDQHTSCPVGQTCCPSLK-------GSWACCQLPHAVCCEDRQHCCPAGYTCNVKARTCEKDVDFIQPPVLLTLGPKVGN 527 HSP 5 Score: 59.3066 bits (142), Expect = 6.380e-10 Identity = 50/161 (31.06%), Postives = 68/161 (42.24%), Query Frame = 1
Query: 148 NKRTKCTDRQTCCQTKHNSYHCCKYANAVCC-------PDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVE 609
+ C + TCC+ ++ CC +A AVCC P F C E TC+ M K+I L++ P IL++ +D CP N T CK ++ CCP AVCCSD CCP+G TC + C K V +E
Sbjct: 296 DMEVSCPEGYTCCRLNTGAWGCCPFAKAVCCEDHIHCCPAGFQCHTEKGTCEMG-------ILQVPWMKKVIAPLRL---PDPQILKSDTPCDDFTRCPTNNTCCKLNS-------GDWGCCPIPEAVCCSDNQHCCPQGFTC-LAQGYCQKGDTMVAGLE 438 HSP 6 Score: 57.3806 bits (137), Expect = 2.794e-9 Identity = 45/164 (27.44%), Postives = 73/164 (44.51%), Query Frame = 1
Query: 103 LLENQKNVTTDNICPNKRTK--CTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSS 588
LL+ +T+ ++ + +T C +C T + CC ++ V C D +HCCP+G C A C ++ D P V CP ++ +C D+ TCCI ++ CCP A CC D+ CCP G +CD+ + +C+ +
Sbjct: 58 LLDTWPRITSHHLDGSCQTHGHCPAGYSCLLTVSGTSSCCPFSKGVSCGDGYHCCPQGFHCSADGKSC----------------FQMSDNPLG-----------AVQCPGSQFECPDSATCCIMVDGSWGCCPMPQASCCEDRVHCCPHGASCDLVHTRCVSPT 194
BLAST of Granulin precursor vs. Ensembl Mouse
Match: Grn (granulin [Source:MGI Symbol;Acc:MGI:95832]) HSP 1 Score: 69.707 bits (169), Expect = 6.645e-14 Identity = 45/158 (28.48%), Postives = 70/158 (44.30%), Query Frame = 1
Query: 148 NKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILE---AKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVEL 612
++ T C QTCC + S+ CC+ +AVCC D+ HCCP G TC+ K C K ++ + P + + PK +E +++ C ++ + CCP+ VCC D CCP G C + KCL+ ++ L
Sbjct: 101 DQHTSCPVGQTCCPSLKGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARTCEK------DVDFIQPPVLLTLGPKVGNVECGEGHFCHDNQTCCKDSA--------------GVWACCPYLKGVCCRDGRHCCPGGFHCSARGTKCLRKKIPRWDMFL 238
BLAST of Granulin precursor vs. Ensembl Mouse
Match: Grn (granulin [Source:MGI Symbol;Acc:MGI:95832]) HSP 1 Score: 64.3142 bits (155), Expect = 1.075e-11 Identity = 50/163 (30.67%), Postives = 72/163 (44.17%), Query Frame = 1
Query: 157 TKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKL--IPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVELPNDAEENGN 639
T+C TCC+ + CC AVCC D HCCP+G TC A+ + C K + + L IPA + + + + CP +T C K ++ CC +AVCC D+ CCP G TC++K + C K + L + GN
Sbjct: 3 TRCPTNNTCCKLNSGDWGCCPIPEAVCCSDNQHCCPQGFTCLAQGY----CQKGDTMVAGLEKIPARQT-----TPLQIGDIGCDQHTSCPVGQTCCPSLK-------GSWACCQLPHAVCCEDRQHCCPAGYTCNVKARTCEKDVDFIQPPVLLTLGPKVGN 149 HSP 2 Score: 50.8322 bits (120), Expect = 3.342e-7 Identity = 24/53 (45.28%), Postives = 32/53 (60.38%), Query Frame = 1
Query: 148 NKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVK 306
++ T C QTCC + S+ CC+ +AVCC D+ HCCP G TC+ K C K
Sbjct: 78 DQHTSCPVGQTCCPSLKGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARTCEK 130
BLAST of Granulin precursor vs. Ensembl Mouse
Match: Grn (granulin [Source:MGI Symbol;Acc:MGI:95832]) HSP 1 Score: 59.3066 bits (142), Expect = 2.392e-10 Identity = 45/162 (27.78%), Postives = 72/162 (44.44%), Query Frame = 1
Query: 103 LLENQKNVTTDNICPNKRTK--CTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLK 582
LL+ +T+ ++ + +T C +C T + CC ++ V C D +HCCP+G C A C ++ D P V CP ++ +C D+ TCCI ++ CCP A CC D+ CCP G +CD+ + +C+
Sbjct: 45 LLDTWPRITSHHLDGSCQTHGHCPAGYSCLLTVSGTSSCCPFSKGVSCGDGYHCCPQGFHCSADGKSC----------------FQMSDNPLG-----------AVQCPGSQFECPDSATCCIMVDGSWGCCPMPQASCCEDRVHCCPHGASCDLVHTRCVS 179 HSP 2 Score: 53.9138 bits (128), Expect = 1.784e-8 Identity = 27/73 (36.99%), Postives = 38/73 (52.05%), Query Frame = 1
Query: 142 CPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVD 360
CP + +C D TCC S+ CC A CC D+ HCCP G++CD +CV ++ + K PA K +
Sbjct: 125 CPGSQFECPDSATCCIMVDGSWGCCPMPQASCCEDRVHCCPHGASCDLVHTRCVSPTGTHTLLKKF-PAQKTN 196
BLAST of Granulin precursor vs. UniProt/SwissProt
Match: sp|P28799|GRN_HUMAN (Progranulin OS=Homo sapiens OX=9606 GN=GRN PE=1 SV=2) HSP 1 Score: 90.5077 bits (223), Expect = 1.070e-19 Identity = 59/191 (30.89%), Postives = 83/191 (43.46%), Query Frame = 1
Query: 52 LIFVVKIYADGVMSFDVLLENQKN-----VTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVE 609
L+ I G L Q+ +++ +CP+ R++C D TCC+ Y CC NA CC D HCCP+ + CD Q KC+ + ++ +PA V D V + EV CP+ T C+ + CCP AVCC D CCP G TCD + C + V +E
Sbjct: 173 LVHTRCITPTGTHPLAKKLPAQRTNRAVALSSSVMCPDARSRCPDGSTCCELPSGKYGCCPMPNATCCSDHLHCCPQDTVCDLIQSKCLSKENATTDLLTKLPAHTVGD----------VKCDMEVSCPDGYTCCRLQS-------GAWGCCPFTQAVCCEDHIHCCPAGFTCDTQKGTCEQGPHQVPWME 346 HSP 2 Score: 87.0409 bits (214), Expect = 1.660e-18 Identity = 61/188 (32.45%), Postives = 85/188 (45.21%), Query Frame = 1
Query: 76 ADGVMSFDVLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVELPNDAEENGN 639
ADG F + N CP+ + +C D TCC S+ CC A CC D+ HCCP G+ CD +C+ + K +PA + + V + VMCP+ +++C D TCC Y CCP NA CCSD CCP+ T CD+ KCL + ++ A G+
Sbjct: 107 ADGRSCFQ---RSGNNSVGAIQCPDSQFECPDFSTCCVMVDGSWGCCPMPQASCCEDRVHCCPHGAFCDLVHTRCIT-PTGTHPLAKKLPAQRTNR---------AVALSSSVMCPDARSRCPDGSTCCELPSGKYGCCPMPNATCCSDHLHCCPQDTVCDLIQSKCLSKENATTDLLTKLPAHTVGD 281 HSP 3 Score: 78.5666 bits (192), Expect = 1.243e-15 Identity = 48/164 (29.27%), Postives = 74/164 (45.12%), Query Frame = 1
Query: 148 NKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNE-----NDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVELPNDA 624
++ T C QTCC + S+ CC+ +AVCC D+ HCCP G TC+ K C K E+ PA + P + + + E +++ C +N + CCP++ VCC+D+ CCP G C + KCL+ + L + A
Sbjct: 445 DQHTSCPVGQTCCPSLGGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARSCEK------EVVSAQPATFLARSPHVGVKDVECGEGHFCHDNQTCCRDN--------------RQGWACCPYRQGVCCADRRHCCPAGFRCAARGTKCLRREAPRWDAPLRDPA 588 HSP 4 Score: 77.0258 bits (188), Expect = 4.436e-15 Identity = 55/171 (32.16%), Postives = 80/171 (46.78%), Query Frame = 1
Query: 97 DVLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVE 609
D+L + + D C + C D TCC+ + ++ CC + AVCC D HCCP G TCD ++ C + M K L + P L+ V ++ CP++ T C+ T + CCP AVCCSD CCP+G TC + +C + S V +E
Sbjct: 269 DLLTKLPAHTVGDVKC-DMEVSCPDGYTCCRLQSGAWGCCPFTQAVCCEDHIHCCPAGFTCDTQKGTCEQGPHQVPWMEKAPAHLSL---PDPQALKRDVPCDNVSSCPSSDTCCQLTS-------GEWGCCPIPEAVCCSDHQHCCPQGYTC-VAEGQCQRGSEIVAGLE 427 HSP 5 Score: 66.6254 bits (161), Expect = 1.776e-11 Identity = 51/179 (28.49%), Postives = 78/179 (43.58%), Query Frame = 1
Query: 115 QKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKL--IPALKVD-DEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVELPNDAEENGNK 642
+++V DN+ + C TCCQ + CC AVCC D HCCP+G TC A+ +C + + + L +PA + P++ + + CP +T C ++ CC +AVCC D+ CCP G TC++K + C K S + G K
Sbjct: 361 KRDVPCDNV-----SSCPSSDTCCQLTSGEWGCCPIPEAVCCSDHQHCCPQGYTCVAEG----QCQRGSEIVAGLEKMPARRASLSHPRD------IGCDQHTSCPVGQTCCPSLG-------GSWACCQLPHAVCCEDRQHCCPAGYTCNVKARSCEKEVVSAQPATFLARSPHVGVK 517 HSP 6 Score: 53.9138 bits (128), Expect = 2.743e-7 Identity = 41/142 (28.87%), Postives = 61/142 (42.96%), Query Frame = 1
Query: 163 CTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSS 588
C+ +C T + CC + AV C D HCCP G C A C + NN + A + CP+++ +C D TCC+ ++ CCP A CC D+ CCP G CD+ + +C+ +
Sbjct: 67 CSAGHSCIFTVSGTSSCCPFPEAVACGDGHHCCPRGFHCSADGRSCFQ-------------------RSGNNSVGA-------IQCPDSQFECPDFSTCCVMVDGSWGCCPMPQASCCEDRVHCCPHGAFCDLVHTRCITPT 182
BLAST of Granulin precursor vs. UniProt/SwissProt
Match: sp|P28797|GRN_CAVPO (Progranulin OS=Cavia porcellus OX=10141 GN=GRN PE=1 SV=2) HSP 1 Score: 89.3521 bits (220), Expect = 2.917e-19 Identity = 61/174 (35.06%), Postives = 88/174 (50.57%), Query Frame = 1
Query: 142 CPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALK-----VDDEP--KNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKS-----------SFSVNNVE 609
CP +C D TCC S+ CC A CC D+ HCCP G++CD +CV S+ KL PA + + P +L A + + V+CP+++++C D TCC+ Y CCP NA+CCSD CCP+ T CD++ +CL S++V +VE
Sbjct: 126 CPGGEFECPDSSTCCHMLDGSWGCCPMPQASCCEDRVHCCPHGASCDLVHIRCVTALGSHPLTTKL-PAQRTNYTGAEGTPVVSPGLLPAALPTS--VICPDSRSQCPDDTTCCLLASGEYGCCPMPNAICCSDHLHCCPQDTVCDLRQSRCLSQNKAKTLLTKLPSWTVWDVE 296 HSP 2 Score: 88.1965 bits (217), Expect = 7.508e-19 Identity = 55/162 (33.95%), Postives = 75/162 (46.30%), Query Frame = 1
Query: 124 VTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVE 609
+ T ICP+ R++C D TCC Y CC NA+CC D HCCP+ + CD +Q +C+ K+ + KL P+ V D V + EV CP +T C+ + CCP AVCC D CCPEG C + C + V +
Sbjct: 216 LPTSVICPDSRSQCPDDTTCCLLASGEYGCCPMPNAICCSDHLHCCPQDTVCDLRQSRCLSQNKAKTLLTKL-PSWTVWD----------VECDQEVSCPEGQTCCRLQS-------GKWGCCPFPKAVCCEDHVHCCPEGFRCHTEKDTCEQGLLQVPWAQ 359 HSP 3 Score: 68.9366 bits (167), Expect = 2.924e-12 Identity = 49/170 (28.82%), Postives = 70/170 (41.18%), Query Frame = 1
Query: 127 TTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNE----NDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVELPNDA 624
TTD C ++ C QTCC + CC+ +AVCC D HCCP G TC+ K C K A + L L V +++ + +DE C + + CCP VCC D+ CCP G C+ + +C+ ++ LP A
Sbjct: 450 TTDVGC-DQHASCPVGQTCCPKLGGGWACCQLPHAVCCEDGQHCCPAGYTCNVKARSCEKAA----DGAHLAAPLAVGS--TGGVMDVACGDRHFCHDEQTCCRDS-------------RGGWACCPFHQGVCCKDQRHCCPAGFHCESQGTRCVHKKSLLHWDSLPRPA 599 HSP 4 Score: 55.8398 bits (133), Expect = 6.895e-8 Identity = 38/144 (26.39%), Postives = 58/144 (40.28%), Query Frame = 1
Query: 163 CTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFS 594
C +C T + CC ++ A+ C D HCCP G C C++ P ++L A E CP++ T C ++ CCP A CC D+ CCP G +CD+ + +C+ + S
Sbjct: 67 CPIGHSCVLTAAGTAACCPFSQAMACGDGHHCCPYGFHCSTDGGTCIQ-------------------RPDIHLLGAVQCPGGEFECPDSSTCCHMLD-------GSWGCCPMPQASCCEDRVHCCPHGASCDLVHIRCVTALGS 184
BLAST of Granulin precursor vs. UniProt/SwissProt
Match: sp|P28798|GRN_MOUSE (Progranulin OS=Mus musculus OX=10090 GN=Grn PE=1 SV=2) HSP 1 Score: 87.4261 bits (215), Expect = 1.368e-18 Identity = 59/160 (36.88%), Postives = 83/160 (51.88%), Query Frame = 1
Query: 142 CPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNV-ELPN 618
CP + +C D TCC S+ CC A CC D+ HCCP G++CD +CV ++ + K PA K + V+ V+CP+ KT+C D TCC Y CCP NA+CCSD CCP+ T CD+ KCL +++ + + +LP
Sbjct: 125 CPGSQFECPDSATCCIMVDGSWGCCPMPQASCCEDRVHCCPHGASCDLVHTRCVSPTGTHTLLKKF-PAQKTNR---------AVSLPFSVVCPDAKTQCPDDSTCCELPTGKYGCCPMPNAICCSDHLHCCPQDTVCDLIQSKCLSKNYTTDLLTKLPG 274 HSP 2 Score: 76.2554 bits (186), Expect = 8.931e-15 Identity = 51/153 (33.33%), Postives = 67/153 (43.79%), Query Frame = 1
Query: 139 ICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSV 597
+CP+ +T+C D TCC+ Y CC NA+CC D HCCP+ + CD Q KC+ + + KL P + E K + EV CP T C+ + CCP AVCC D CCP G C + C V
Sbjct: 206 VCPDAKTQCPDDSTCCELPTGKYGCCPMPNAICCSDHLHCCPQDTVCDLIQSKCLSKNYTTDLLTKL---------PGYPVKEVKCDM--EVSCPEGYTCCRLNT-------GAWGCCPFAKAVCCEDHIHCCPAGFQCHTEKGTCEMGILQV 340 HSP 3 Score: 69.707 bits (169), Expect = 1.645e-12 Identity = 45/158 (28.48%), Postives = 70/158 (44.30%), Query Frame = 1
Query: 148 NKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILE---AKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVEL 612
++ T C QTCC + S+ CC+ +AVCC D+ HCCP G TC+ K C K ++ + P + + PK +E +++ C ++ + CCP+ VCC D CCP G C + KCL+ ++ L
Sbjct: 443 DQHTSCPVGQTCCPSLKGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARTCEK------DVDFIQPPVLLTLGPKVGNVECGEGHFCHDNQTCCKDSA--------------GVWACCPYLKGVCCRDGRHCCPGGFHCSARGTKCLRKKIPRWDMFL 580 HSP 4 Score: 67.0106 bits (162), Expect = 1.182e-11 Identity = 53/182 (29.12%), Postives = 81/182 (44.51%), Query Frame = 1
Query: 100 VLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKL--IPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVELPNDAEENGN 639
+ L + + + +D C + T+C TCC+ + CC AVCC D HCCP+G TC A+ + C K + + L IPA + + + + CP +T C K ++ CC +AVCC D+ CCP G TC++K + C K + L + GN
Sbjct: 350 LRLPDPQILKSDTPC-DDFTRCPTNNTCCKLNSGDWGCCPIPEAVCCSDNQHCCPQGFTCLAQGY----CQKGDTMVAGLEKIPARQT-----TPLQIGDIGCDQHTSCPVGQTCCPSLK-------GSWACCQLPHAVCCEDRQHCCPAGYTCNVKARTCEKDVDFIQPPVLLTLGPKVGN 514 HSP 5 Score: 59.3066 bits (142), Expect = 4.602e-9 Identity = 50/161 (31.06%), Postives = 68/161 (42.24%), Query Frame = 1
Query: 148 NKRTKCTDRQTCCQTKHNSYHCCKYANAVCC-------PDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVE 609
+ C + TCC+ ++ CC +A AVCC P F C E TC+ M K+I L++ P IL++ +D CP N T CK ++ CCP AVCCSD CCP+G TC + C K V +E
Sbjct: 283 DMEVSCPEGYTCCRLNTGAWGCCPFAKAVCCEDHIHCCPAGFQCHTEKGTCEMG-------ILQVPWMKKVIAPLRL---PDPQILKSDTPCDDFTRCPTNNTCCKLNS-------GDWGCCPIPEAVCCSDNQHCCPQGFTC-LAQGYCQKGDTMVAGLE 425 HSP 6 Score: 57.3806 bits (137), Expect = 1.960e-8 Identity = 45/164 (27.44%), Postives = 73/164 (44.51%), Query Frame = 1
Query: 103 LLENQKNVTTDNICPNKRTK--CTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSS 588
LL+ +T+ ++ + +T C +C T + CC ++ V C D +HCCP+G C A C ++ D P V CP ++ +C D+ TCCI ++ CCP A CC D+ CCP G +CD+ + +C+ +
Sbjct: 45 LLDTWPRITSHHLDGSCQTHGHCPAGYSCLLTVSGTSSCCPFSKGVSCGDGYHCCPQGFHCSADGKSC----------------FQMSDNPLG-----------AVQCPGSQFECPDSATCCIMVDGSWGCCPMPQASCCEDRVHCCPHGASCDLVHTRCVSPT 181
BLAST of Granulin precursor vs. UniProt/SwissProt
Match: sp|P23785|GRN_RAT (Progranulin OS=Rattus norvegicus OX=10116 GN=Grn PE=1 SV=3) HSP 1 Score: 84.7297 bits (208), Expect = 1.083e-17 Identity = 57/160 (35.62%), Postives = 81/160 (50.62%), Query Frame = 1
Query: 142 CPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNV-ELPN 618
CP + +C D TCC S+ CC A CC D+ HCCP G++CD +C+ ++ P LK + N A + V+CP+ KT+C D TCC Y CCP NA+CCSD CCP+ T CD+ KC+ ++ + + +LP
Sbjct: 125 CPGSQFECPDSATCCIMIDGSWGCCPMPQASCCEDRVHCCPHGASCDLVHTRCISPTGTH-------PLLKKFPAQRTNRAVASFS----VVCPDAKTQCPDDSTCCELPTGKYGCCPMPNAICCSDHLHCCPQDTVCDLIQSKCISKDYTTDLMTKLPG 273 HSP 2 Score: 80.4925 bits (197), Expect = 2.759e-16 Identity = 54/168 (32.14%), Postives = 73/168 (43.45%), Query Frame = 1
Query: 94 FDVLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSV 597
F N+ + +CP+ +T+C D TCC+ Y CC NA+CC D HCCP+ + CD Q KC+ + M KL P V++ V + EV CP+ T C+ + CCP AVCC D CCP G C + C V
Sbjct: 190 FPAQRTNRAVASFSVVCPDAKTQCPDDSTCCELPTGKYGCCPMPNAICCSDHLHCCPQDTVCDLIQSKCISKDYTTDLMTKL-PGYPVNE----------VKCDLEVSCPDGYTCCRLNT-------GAWGCCPFTKAVCCEDHIHCCPAGFQCHTETGTCELGVLQV 339 HSP 3 Score: 73.559 bits (179), Expect = 8.047e-14 Identity = 50/161 (31.06%), Postives = 73/161 (45.34%), Query Frame = 1
Query: 148 NKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVELPNDAEE 630
++ T C QTCC + S+ CC+ +AVCC D+ HCCP G TC+ K C K + G + P++ + K +E C +N ++CC + CCP+ VCC D CCP G C K KCL+ ++ L + A
Sbjct: 442 DQHTSCPVGQTCCPSLKGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARTCEK------DAGSVQPSMDLTFGSKVGNVECGAGH----FCHDN-------QSCCKDSQGGWACCPYVKGVCCRDGRHCCPIGFHCSAKGTKCLRKKTPRWDILLRDPAPR 585 HSP 4 Score: 61.6178 bits (148), Expect = 7.541e-10 Identity = 51/186 (27.42%), Postives = 86/186 (46.24%), Query Frame = 1
Query: 46 LLLIFVVKIYADGVMSFDVLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILE-AKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVN 600
L + ++ K+ A + +L+N +V D+ + C TCC+ + CC AVCC D HCCP+G C + + C K + +++ L+ + +L+ + + CP +TCC + ++ CC +AVCC D+ CCP G TC++K + C K + SV
Sbjct: 337 LQVPWMKKVTASLSLPDPQILKN--DVPCDDF-----SSCPSNNTCCRLSSGDWGCCPMPEAVCCLDHQHCCPQGFKCMDEGY----CQKGD----RMVAGLEKMPVRQTTLLQHGDIGCDQHTSCPVG-------QTCCPSLKGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARTCEKDAGSVQ 500 HSP 5 Score: 56.9954 bits (136), Expect = 3.243e-8 Identity = 48/165 (29.09%), Postives = 72/165 (43.64%), Query Frame = 1
Query: 103 LLENQKNVTT---DNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSS 588
LL+ +T+ D C R C D +C T + CC ++ V C D HCCP G C A C + +++L A V CP ++ +C D+ TCCI ++ CCP A CC D+ CCP G +CD+ + +C+ +
Sbjct: 45 LLDTWPIITSRRLDGSC-QIRDHCPDGYSCLLTVSGTSSCCPFSEGVSCDDGQHCCPRGFHCSADGKSC--------------------SQISDSLLGA-------VQCPGSQFECPDSATCCIMIDGSWGCCPMPQASCCEDRVHCCPHGASCDLVHTRCISPT 181 HSP 6 Score: 53.1434 bits (126), Expect = 6.492e-7 Identity = 47/161 (29.19%), Postives = 66/161 (40.99%), Query Frame = 1
Query: 148 NKRTKCTDRQTCCQTKHNSYHCCKYANAVCC-------PDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVE 609
+ C D TCC+ ++ CC + AVCC P F C E TC+ M K+ +L + P IL+ V +D CP+N T C+ + ++ CCP AVCC D CCP+G C + C K V +E
Sbjct: 282 DLEVSCPDGYTCCRLNTGAWGCCPFTKAVCCEDHIHCCPAGFQCHTETGTCELG-------VLQVPWMKKVTASLSL---PDPQILKNDVPCDDFSSCPSNNTCCRLSS-------GDWGCCPMPEAVCCLDHQHCCPQGFKC-MDEGYCQKGDRMVAGLE 424
BLAST of Granulin precursor vs. UniProt/SwissProt
Match: sp|P80059|PMD1_LOCMI (Pars intercerebralis major peptide D1 OS=Locusta migratoria OX=7004 PE=1 SV=1) HSP 1 Score: 46.9802 bits (110), Expect = 2.624e-6 Identity = 20/48 (41.67%), Postives = 25/48 (52.08%), Query Frame = 1
Query: 163 CTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVK 306
C +TCC T CC Y VCC D HCCP G+ CD +C++
Sbjct: 7 CPGTETCCTTPQGEEGCCPYKEGVCCLDGIHCCPSGTVCDEDHRRCIQ 54
BLAST of Granulin precursor vs. TrEMBL
Match: A0A2U9CNI5 (Putative granulins-like OS=Scophthalmus maximus OX=52904 GN=SMAX5B_020588 PE=4 SV=1) HSP 1 Score: 113.235 bits (282), Expect = 5.256e-25 Identity = 68/178 (38.20%), Postives = 93/178 (52.25%), Query Frame = 1
Query: 43 NLLLIFVVKIYADGVMSFDVLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKC 576
+ L ++ ++ A + VL EN K + +C ++++ C D TCCQ S+ CC AVCC DK HCCPEG+ CD + CV + + M K +PA + EN+ V CP+ C D TCC + +Y CCP NAVCCSD CCPEGT CD+K+ C
Sbjct: 77 TVSLPWMRRLPAQPALPVAVLGENVKKI----VCADQKSTCDDGSTCCQLLDGSWGCCPLVKAVCCEDKLHCCPEGTKCDTAREMCVSASLESFPMLKKLPAGR--------------RENNPVQCPDKTMTCPDQTTCCQMTNGSYGCCPMPNAVCCSDHMHCCPEGTQCDLKHSTC 236 HSP 2 Score: 85.1149 bits (209), Expect = 2.814e-15 Identity = 53/145 (36.55%), Postives = 65/145 (44.83%), Query Frame = 1
Query: 142 CPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKC 576
CP+K C D+ TCCQ + SY CC NAVCC D HCCPEG+ CD K C + + ++ A V ND V C + TCC + CCP AVCC D CCP GT C++ C
Sbjct: 184 CPDKTMTCPDQTTCCQMTNGSYGCCPMPNAVCCSDHMHCCPEGTQCDLKHSTCALAHRDAIMASRITAA-------ATKSRGGSVPCNDSVSCADG-------STCCKMLDGEWVCCPLPQAVCCDDHEHCCPHGTVCNLAASTC 314 HSP 3 Score: 82.8037 bits (203), Expect = 1.607e-14 Identity = 65/174 (37.36%), Postives = 86/174 (49.43%), Query Frame = 1
Query: 127 TTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLI--PALKVDDEPKNNILEAKVNEN-DEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNV--ELPNDAEEN 633
+T +CP+ C DR TCC+ Y CC +A CC D HCC EG+ CD +CV S M +L PAL V A + EN +++C + K+ C D TCC ++ CCP AVCC DK CCPEGT CD + C+ +S + +LP EN
Sbjct: 17 STALVCPDG-GMCEDRNTCCKNTVGGYGCCPLPHAECCSDHLHCCYEGTLCDLVHEQCVNKTVSLPWMRRLPAQPALPV----------AVLGENVKKIVCADQKSTCDDGSTCCQLLDGSWGCCPLVKAVCCEDKLHCCPEGTKCDTAREMCVSASLESFPMLKKLPAGRREN 179 HSP 4 Score: 68.5514 bits (166), Expect = 1.247e-9 Identity = 57/179 (31.84%), Postives = 84/179 (46.93%), Query Frame = 1
Query: 94 FDVLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNIL--EAKVNENDEVMCPN-NXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVELPND 621
L E TD C +K + C TCC+ + CC AVCC D+ HCCP+G TC+ + C K N++ G + ++ P+ ++ E+ E+D+V C + +C +TCC T ++ CCP A CCSD CCP G +CD C ++ N L D
Sbjct: 578 LSALTEPGAMTVTDVKCDDK-SSCASGTTCCKLSTGEWGCCPLVKAVCCADQEHCCPQGYTCNMQTG---TCEKKNLDDGLVQASV-----PQTQVVRPESGHPEDDDVPCDSAGSVRCSGQETCCKTSPTDWACCPSPRATCCSDLKHCCPVGYSCDPTAGGC-----TLENARLTWD 742 HSP 5 Score: 68.1662 bits (165), Expect = 1.491e-9 Identity = 55/194 (28.35%), Postives = 82/194 (42.27%), Query Frame = 1
Query: 82 GVMSFDVLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPA---------LKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVELPNDAEENG 636
G+++ E + +D C +++T C TCC+ + CC AVCC D HCCP G CD + C K + + IPA K + + ++L ++ D MC + + +DT C + + + + CCP AVCC D CCP G TC C K V + E G
Sbjct: 397 GLLAPLRWTEKVAALRSDVQC-DEQTSCPGGTTCCKRSSGEWSCCPLPQAVCCDDHEHCCPAGYLCDLAEQTCDKPGGVRLPWLRKIPALLQLHQQQRQKNEPDRAGSVLPSR----DRNMCDAHTSCPRDTTCCFMDQSHRWGCCPLPKAVCCDDGNHCCPAGHTCQPHRSSCSKGPQVVPWFSKLSALTEPG 585 HSP 6 Score: 67.781 bits (164), Expect = 1.835e-9 Identity = 50/156 (32.05%), Postives = 65/156 (41.67%), Query Frame = 1
Query: 148 NKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVELP 615
+K T C TCC+T + CC AVCC D HCCP + C+ C + G L P + K L + V +++ CP T CK + + CCP AVCC D CCP G CD+ + C K V LP
Sbjct: 342 DKSTSCPGNSTCCRTTTGDWACCPLPEAVCCDDHIHCCPHATVCNLDFDTC------DDPSGLLAPLRWTE---KVAALRSDVQCDEQTSCPGGTTCCKRSS-------GEWSCCPLPQAVCCDDHEHCCPAGYLCDLAEQTCDKP----GGVRLP 477 HSP 7 Score: 60.077 bits (144), Expect = 8.716e-7 Identity = 49/159 (30.82%), Postives = 71/159 (44.65%), Query Frame = 1
Query: 136 NICPNKRTKCTDRQTCC-QTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVE 609
N+C + T C TCC + + + CC AVCC D HCCP G TC + C K ++ L EP + V +D+ C + T CK + + CCP AVCC+D+ CCP+G TC+++ C K + V+
Sbjct: 511 NMC-DAHTSCPRDTTCCFMDQSHRWGCCPLPKAVCCDDGNHCCPAGHTCQPHRSSCSK----GPQVVPWFSKLSALTEP-GAMTVTDVKCDDKSSCASGTTCCKLST-------GEWGCCPLVKAVCCADQEHCCPQGYTCNMQTGTCEKKNLDDGLVQ 656
BLAST of Granulin precursor vs. TrEMBL
Match: A0A5N5Q463 (Uncharacterized protein OS=Pangasianodon hypophthalmus OX=310915 GN=PHYPO_G00004620 PE=4 SV=1) HSP 1 Score: 112.464 bits (280), Expect = 8.503e-25 Identity = 65/174 (37.36%), Postives = 97/174 (55.75%), Query Frame = 1
Query: 103 LLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVN--ENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKC-LKSSFSVNNVELP 615
++ Q + +CP++ ++C D TCCQ ++ CC NAVCC DK HCCP G+TCD +CV + + + PA+ V + + E V ++++V+CP+ + C D TCCI + +Y CCP NAVCC D CCPEGTTCD+++ C L + + E P
Sbjct: 570 VVAKQTTLPQAVVCPDQESECPDDTTCCQMPDGTWGCCPMPNAVCCEDKRHCCPAGTTCDLPHSRCVSSTLGSTPLLRKFPAIHVGGKRDAVVKEISVKFPDSNDVVCPDKVSTCPDDTTCCILANGSYGCCPLPNAVCCDDYVHCCPEGTTCDLEHSTCVLANELTTMTAEAP 743 HSP 2 Score: 87.8113 bits (216), Expect = 3.311e-16 Identity = 59/168 (35.12%), Postives = 79/168 (47.02%), Query Frame = 1
Query: 139 ICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVELPNDAEENGNK 642
ICP+ C D TCCQT Y CC NA CC D HCC EG+ CD + CV +E + + A + + +A V + E CP++ T C+ + CCP NAVCC DK CCP GTTCD+ + +C+ S+ + A G K
Sbjct: 505 ICPDG-GMCDDGNTCCQTSGGGYGCCPLPNAECCSDHLHCCHEGTLCDMEHGTCVN-KTHTLEWVRRVVA------KQTTLPQAVVCPDQESECPDDTTCCQMPD-------GTWGCCPMPNAVCCEDKRHCCPAGTTCDLPHSRCVSSTLGSTPLLRKFPAIHVGGK 657 HSP 3 Score: 87.4261 bits (215), Expect = 3.748e-16 Identity = 57/151 (37.75%), Postives = 71/151 (47.02%), Query Frame = 1
Query: 133 DNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNE---NDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKC 576
D +CP+K + C D TCC + SY CC NAVCC D HCCPEG+TCD + CV + + P L +L+ V+ ND V C T CK + + CCP AVCC D CCP GT CD+ C
Sbjct: 674 DVVCPDKVSTCPDDTTCCILANGSYGCCPLPNAVCCDDYVHCCPEGTTCDLEHSTCVLANELTTMTAEAPPIL---------LLKPTVDSVPCNDTVACATGFTCCKKQD-------SEWGCCPLPEAVCCEDHIHCCPHGTVCDLSEGTC 808 HSP 4 Score: 81.6481 bits (200), Expect = 4.768e-14 Identity = 55/144 (38.19%), Postives = 74/144 (51.39%), Query Frame = 1
Query: 157 TKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSS 588
T C TCC+ + + CC AVCC D HCCP+G TCD + C+K + ++ L+ L DE DEVMC + +C ++TCC N + CCP + AVCC D CCP+G TCD K+C K+S
Sbjct: 1071 TSCAAGTTCCRLRTGKWGCCPLVKAVCCEDHEHCCPQGYTCDLQSGTCIKPSTTHTLALTLVHTLSEQDE-------------DEVMC-DATRRCPKSQTCCRIPDNEWACCPFEQAVCCKDMKHCCPKGYTCD-PEKRCTKAS 1199 HSP 5 Score: 72.0182 bits (175), Expect = 7.068e-11 Identity = 50/159 (31.45%), Postives = 72/159 (45.28%), Query Frame = 1
Query: 148 NKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDD----EPKNNILEAKVNEND-EVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVE 609
++ + C TCC+ K ++ CC+ AVCC D HCCP+G CD C + ++ + PAL V +P N+ ++ D + CP T C K + + CCP AVCC D CCP G TCD + C K + + E
Sbjct: 894 DETSACPTGTTCCKEKSGTWACCQLPRAVCCEDHEHCCPQGYKCDVAHKSCEHPSLPSMPWVRKQPALSVTHSSYADPSANMESQHGHKCDAQTTCPRGNTCCYMAKL------SKWGCCPLPQAVCCGDGDHCCPSGYTCDKEKPVCTKGNLQIAWFE 1046 HSP 6 Score: 68.9366 bits (167), Expect = 8.632e-10 Identity = 52/149 (34.90%), Postives = 74/149 (49.66%), Query Frame = 1
Query: 148 NKRTKCTDRQTCC-QTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNEN-DEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSS 588
+ +T C TCC K + + CC AVCC D HCCP G TCD ++ C K N+++ + +P ++V +V C +N T C TCC + + CCP AVCC D CCP+G TCD+++ C+K S
Sbjct: 984 DAQTTCPRGNTCCYMAKLSKWGCCPLPQAVCCGDGDHCCPSGYTCDKEKPVCT---KGNLQIAWF------EKQPAMIAQGSEVTRGLGDVKC-DNTTSCAAGTTCCRLRTGKWGCCPLVKAVCCEDHEHCCPQGYTCDLQSGTCIKPS 1122
BLAST of Granulin precursor vs. TrEMBL
Match: A0A3P8XNX3 (Granulin b OS=Esox lucius OX=8010 PE=4 SV=1) HSP 1 Score: 112.079 bits (279), Expect = 9.721e-25 Identity = 71/195 (36.41%), Postives = 98/195 (50.26%), Query Frame = 1
Query: 34 LGFNLLLIFVVKIYADGVMSFDVLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCL-KSSFSVNNVELP 615
L ++ L +V ++ + S ++LE K V +CP+ +C D TCCQ S+ CC A AVCC DK HCCPEG+TCD KCV M K +P+ K + + + +CP+ TCC Y CCP+ AVCCSDK CCPEGTTCD++ CL ++ + V+ P
Sbjct: 77 LNRSVSLPWVSRVPSK-QPSVPLILEGVKAV----VCPDGEAECPDGTTCCQLPEGSWGCCPLAKAVCCQDKRHCCPEGTTCDLVHSKCVSPTLGTFPMSKKVPSRKKQTAVGGQTVTSPGGKG---VCPDG-------FTCCQQPSGEYGCCPYLEAVCCSDKLHCCPEGTTCDLEESTCLSQNGLTSMAVKFP 256 HSP 2 Score: 92.4337 bits (228), Expect = 7.802e-18 Identity = 65/186 (34.95%), Postives = 92/186 (49.46%), Query Frame = 1
Query: 40 FNLLLIFVVKIYADGVMSFDVLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSV 597
N +L V G + VL +T+ ICP+ R C + TCCQT Y CC NA CC D HCC EG+ CD +C+ + S + + +P+ +P ++ V V+CP+ + +C D TCC ++ CCP AVCC DK CCPEGTTCD+ + KC+ +
Sbjct: 1 MNTMLTLWV-----GAVCLAVL-----GLTSALICPDGRM-CEEGNTCCQTSSGEYGCCPLPNAECCSDHLHCCYEGTVCDLALARCLNRSVSLPWVSR-VPS----KQPSVPLILEGVK---AVVCPDGEAECPDGTTCCQLPEGSWGCCPLAKAVCCQDKRHCCPEGTTCDLVHSKCVSPTLGT 167 HSP 3 Score: 76.6406 bits (187), Expect = 1.855e-12 Identity = 57/175 (32.57%), Postives = 77/175 (44.00%), Query Frame = 1
Query: 118 KNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPAL--KVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVELPNDAEENG 636
+ + DN C ++ T C TCC+T + CC NAVCC D HCCP G+ C+ K C A S+V +P L + +++ AK + CP + T C + K + CCP AVCCSD CCP G CD C K +V + E G
Sbjct: 344 SHASKDNRC-DESTSCPGEYTCCKTATGKWACCPLPNAVCCDDLIHCCPHGTVCNLKASTCDDPAGSSVPWVPKLPGLHPGLTQASASSVPPAKNMCDPHTSCPKD------TTCCFMNKSGKWGCCPLPKAVCCSDGDHCCPSGYICDDHKGTCTKGLATVPWYPKESALTEEG 511 HSP 4 Score: 65.855 bits (159), Expect = 8.681e-9 Identity = 44/143 (30.77%), Postives = 57/143 (39.86%), Query Frame = 1
Query: 148 NKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKC 576
N C D TCC+ + CC AVCC D HCCP + C+ + C + + L A K+N + + E C C T + CCP NAVCC D CCP GT C++K C
Sbjct: 274 NGSVACADGTTCCKLVDGQWACCPLPKAVCCDDHLHCCPHDTVCNLAEGTCDDPSSGSALATLLDKAPAFSHASKDNRCDESTSCPGEYTC-------------CKTATGKWACCPLPNAVCCDDLIHCCPHGTVCNLKASTC 403
BLAST of Granulin precursor vs. TrEMBL
Match: W5LK03 (Granulin b OS=Astyanax mexicanus OX=7994 PE=4 SV=2) HSP 1 Score: 111.309 bits (277), Expect = 2.300e-24 Identity = 65/172 (37.79%), Postives = 98/172 (56.98%), Query Frame = 1
Query: 79 DGVMSFDVLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALK-VDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSF 591
D VM + Q ++ +CP++ ++C D TCCQ S+ CC NAVCC DK HCCP+G+TCD KCV +V + + PA K + + + + + ++++V CP+ + C D TCC + +Y CCP AVCCSD CCP+GT+CD+ + KC+ S+
Sbjct: 79 DWVMRVPI---RQPSLPQAVVCPDQESECPDDTTCCQLPDGSWGCCPMPNAVCCEDKMHCCPQGTTCDLPHSKCVSSMLGSVPLRRKFPAYKRAEWKARKDSNGLDLADSNDVDCPDKVSFCPDDTTCCPLGNGSYGCCPMPKAVCCSDHVHCCPQGTSCDLAHSKCVSSNV 247 HSP 2 Score: 88.1965 bits (217), Expect = 2.204e-16 Identity = 58/152 (38.16%), Postives = 77/152 (50.66%), Query Frame = 1
Query: 139 ICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFS 594
ICP+ C D TCCQT Y CC NA CC D HCC EG+ CD + KCV ++ +P + ++ +A V + E CP++ TCC ++ CCP NAVCC DK CCP+GTTCD+ + KC+ S
Sbjct: 19 ICPDGGM-CDDGNTCCQTPSGGYGCCPLPNAECCSDHLHCCHEGTLCDLEHSKCVN-KTHTLDWVMRVPIR------QPSLPQAVVCPDQESECPDD-------TTCCQLPDGSWGCCPMPNAVCCEDKMHCCPQGTTCDLPHSKCVSSMLG 155 HSP 3 Score: 77.7962 bits (190), Expect = 7.455e-13 Identity = 54/141 (38.30%), Postives = 68/141 (48.23%), Query Frame = 1
Query: 133 DNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTC 555
D CP+K + C D TCC + SY CC AVCC D HCCP+G++CD KCV L P ++V + V N+ V C + TCC TK ++ CCP AVCC D CCP+GT C
Sbjct: 187 DVDCPDKVSFCPDDTTCCPLGNGSYGCCPMPKAVCCSDHVHCCPQGTSCDLAHSKCVSSNVLEPVTTNLSPVVRV------KVTVESVPCNESVACESG-------STCCKTKDGDWACCPLPEAVCCEDHIHCCPQGTVC 314 HSP 4 Score: 72.0182 bits (175), Expect = 7.042e-11 Identity = 60/170 (35.29%), Postives = 76/170 (44.71%), Query Frame = 1
Query: 148 NKRTKCTDRQTCC-QTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKL-IPALKVDDEPKNNILEAKVNENDEVM-------------CPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVEL 612
+ +T C TCC K N + CC AVCC D HCCP TCD ++ C+K G L IP K EAKV + EV CP T C+ T + CCPH AVCC D CCP G TCD++ CL++S +++ V L
Sbjct: 528 DAQTSCPRDNTCCYMKKFNKWGCCPLPEAVCCGDGDHCCPNSYTCDTTKNTCLK--------GHLEIPWFKKQ--------EAKVTQGSEVAHGVADVQCDSTARCPTGSTCCRLTT-------GQWGCCPHVQAVCCDDHVHCCPAGYTCDLQAGTCLRTS-TLHTVAL 673 HSP 5 Score: 70.4774 bits (171), Expect = 2.407e-10 Identity = 53/165 (32.12%), Postives = 76/165 (46.06%), Query Frame = 1
Query: 124 VTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNE--NDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVEL 612
VT +++ N+ C TCC+TK + CC AVCC D HCCP+G+ C + CV ++ ++ L+ V+ +P N + NE + CP T CK + CCP AVCC D CCP T C++ C S+FS +L
Sbjct: 263 VTVESVPCNESVACESGSTCCKTKDGDWACCPLPEAVCCEDHIHCCPQGTVC--ARSTCV----ASADLLTLV--RWVEKKPTINRVPQPANEKCDPTTTCPKGATCCKMES-------GTWGCCPLPQAVCCEDHLHCCPRNTVCNVAAGTC-DSAFSGTAGQL 411 HSP 6 Score: 68.9366 bits (167), Expect = 7.908e-10 Identity = 46/147 (31.29%), Postives = 69/147 (46.94%), Query Frame = 1
Query: 148 NKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSS 588
+ +C TCC+ + CC + AVCC D HCCP G TCD + C++ + + L+ + ++VMC + + C +++TCC + CCP K AVCC D CCP G CD K K C +++
Sbjct: 612 DSTARCPTGSTCCRLTTGQWGCCPHVQAVCCDDHVHCCPAGYTCDLQAGTCLRTSTLHTVALTLV----------------HTHTKEQVMC-DATSHCTESQTCCKLSDTEWACCPFKQAVCCKDMKHCCPVGYVCDPKAKGCARAT 741 HSP 7 Score: 67.0106 bits (162), Expect = 3.713e-9 Identity = 50/161 (31.06%), Postives = 69/161 (42.86%), Query Frame = 1
Query: 148 NKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKN----NILEAKVNE-------NDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSV 597
++ + C TCC+ K ++ CC AVCC D HCCP+G CD C ++ K PAL+ + N I E+ V + + + CP + C + K N + CCP AVCC D CCP TCD CLK +
Sbjct: 432 DETSACPTGTTCCRQKSGAWACCPLPQAVCCDDHEHCCPQGYKCDIIHESCDHPFLPSMLWFKKQPALQSMTQQSNANAKAIAESAVADVESKHKCDAQTSCPRD------NTCCYMKKFNKWGCCPLPEAVCCGDGDHCCPNSYTCDTTKNTCLKGHLEI 586
BLAST of Granulin precursor vs. TrEMBL
Match: A0A3P8XNU6 (Granulin b OS=Esox lucius OX=8010 PE=4 SV=1) HSP 1 Score: 109.768 bits (273), Expect = 5.426e-24 Identity = 72/189 (38.10%), Postives = 97/189 (51.32%), Query Frame = 1
Query: 64 VKIYADGVMSFDVLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVM----CPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCL-KSSFSVNNVELP 615
V+++ + V+ +LE K V +CP+ +C D TCCQ S+ CC A AVCC DK HCCPEG+TCD KCV P L K N + N + CP+ + C + TCC+ + +Y CCP NAVCCSDK CCPEGTTCD++ CL ++ + V+ P
Sbjct: 49 VRLWPE-VIQLLGILEGVKAV----VCPDGEAECPDGTTCCQLPEGSWGCCPLAKAVCCQDKRHCCPEGTTCDLVHSKCVS------------PTLGTFPMSKKNTFNSGSNLYSLLFSVLECPDKLSSCPEDSTCCVLEDGSYGCCPMPNAVCCSDKLHCCPEGTTCDLEESTCLSQNGLTSMAVKFP 220 HSP 2 Score: 68.1662 bits (165), Expect = 1.205e-9 Identity = 60/206 (29.13%), Postives = 84/206 (40.78%), Query Frame = 1
Query: 118 KNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYAN--------------------AVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPAL--KVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFS--VNNVELPNDAEENGNK*YIEYLL 663
+ + DN C ++ T C TCC+T + CC N AVCC D HCCP G+ C+ K C A S+V +P L + +++ AK + CP + T C K+ + CCP AVCCSD CCP G CD C K+ F V ++ L A + ++ Y L
Sbjct: 308 SHASKDNRC-DESTSCPGEYTCCKTATGKWACCPLPNVNAFPHFLCPVGQCRRQTPDAVCCDDLIHCCPHGTVCNLKASTCDDPAGSSVPWVPKLPGLHPGLTQASASSVPPAKNMCDPHTSCPKDTTCCFMNKS------GKWGCCPLPKAVCCSDGDHCCPSGYICDDHKGTCTKAPFWELVEHLWLLQTARD-----FLGYFL 501 HSP 3 Score: 58.5362 bits (140), Expect = 2.807e-6 Identity = 48/173 (27.75%), Postives = 67/173 (38.73%), Query Frame = 1
Query: 121 NVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVELPNDAEENGN 639
+T+ ICP+ R C + TCCQT Y CC +P + V P+ L + V+CP+ + +C D TCC ++ CCP AVCC DK CCPEGTTCD+ + KC+ + + N N
Sbjct: 12 GLTSALICPDGR-MCEEGNTCCQTSSGEYGCCP----------------------------------------LPNVSVRLWPEVIQLLGILEGVKAVVCPDGEAECPDGTTCCQLPEGSWGCCPLAKAVCCQDKRHCCPEGTTCDLVHSKCVSPTLGTFPMSKKNTFNSGSN 143 HSP 4 Score: 57.7658 bits (138), Expect = 4.438e-6 Identity = 41/150 (27.33%), Postives = 56/150 (37.33%), Query Frame = 1
Query: 148 NKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCP-------HKNAVCCSDKYQCCPEGTTCDIKNKKC 576
N C D TCC+ + CC AVCC D HCCP + C+ + C + + L A K+N + + E C T ++ CP +AVCC D CCP GT C++K C
Sbjct: 238 NGSVACADGTTCCKLVDGQWACCPLPKAVCCDDHLHCCPHDTVCNLAEGTCDDPSSGSALATLLDKAPAFSHASKDNRCDESTSCPGEYTCCKTATGKWACCPLPNVNAFPHFLCPVGQCRRQTPDAVCCDDLIHCCPHGTVCNLKASTC 387
BLAST of Granulin precursor vs. Ensembl Cavefish
Match: grnb (granulins-like [Source:NCBI gene;Acc:103046308]) HSP 1 Score: 111.309 bits (277), Expect = 8.712e-28 Identity = 65/172 (37.79%), Postives = 98/172 (56.98%), Query Frame = 1
Query: 79 DGVMSFDVLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALK-VDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSF 591
D VM + Q ++ +CP++ ++C D TCCQ S+ CC NAVCC DK HCCP+G+TCD KCV +V + + PA K + + + + + ++++V CP+ + C D TCC + +Y CCP AVCCSD CCP+GT+CD+ + KC+ S+
Sbjct: 79 DWVMRVPI---RQPSLPQAVVCPDQESECPDDTTCCQLPDGSWGCCPMPNAVCCEDKMHCCPQGTTCDLPHSKCVSSMLGSVPLRRKFPAYKRAEWKARKDSNGLDLADSNDVDCPDKVSFCPDDTTCCPLGNGSYGCCPMPKAVCCSDHVHCCPQGTSCDLAHSKCVSSNV 247 HSP 2 Score: 88.1965 bits (217), Expect = 8.348e-20 Identity = 58/152 (38.16%), Postives = 77/152 (50.66%), Query Frame = 1
Query: 139 ICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFS 594
ICP+ C D TCCQT Y CC NA CC D HCC EG+ CD + KCV ++ +P + ++ +A V + E CP++ TCC ++ CCP NAVCC DK CCP+GTTCD+ + KC+ S
Sbjct: 19 ICPDGGM-CDDGNTCCQTPSGGYGCCPLPNAECCSDHLHCCHEGTLCDLEHSKCVN-KTHTLDWVMRVPIR------QPSLPQAVVCPDQESECPDD-------TTCCQLPDGSWGCCPMPNAVCCEDKMHCCPQGTTCDLPHSKCVSSMLG 155 HSP 3 Score: 77.7962 bits (190), Expect = 2.824e-16 Identity = 54/141 (38.30%), Postives = 68/141 (48.23%), Query Frame = 1
Query: 133 DNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTC 555
D CP+K + C D TCC + SY CC AVCC D HCCP+G++CD KCV L P ++V + V N+ V C + TCC TK ++ CCP AVCC D CCP+GT C
Sbjct: 187 DVDCPDKVSFCPDDTTCCPLGNGSYGCCPMPKAVCCSDHVHCCPQGTSCDLAHSKCVSSNVLEPVTTNLSPVVRV------KVTVESVPCNESVACESG-------STCCKTKDGDWACCPLPEAVCCEDHIHCCPQGTVC 314 HSP 4 Score: 72.0182 bits (175), Expect = 2.667e-14 Identity = 60/170 (35.29%), Postives = 76/170 (44.71%), Query Frame = 1
Query: 148 NKRTKCTDRQTCC-QTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKL-IPALKVDDEPKNNILEAKVNENDEVM-------------CPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVEL 612
+ +T C TCC K N + CC AVCC D HCCP TCD ++ C+K G L IP K EAKV + EV CP T C+ T + CCPH AVCC D CCP G TCD++ CL++S +++ V L
Sbjct: 528 DAQTSCPRDNTCCYMKKFNKWGCCPLPEAVCCGDGDHCCPNSYTCDTTKNTCLK--------GHLEIPWFKKQ--------EAKVTQGSEVAHGVADVQCDSTARCPTGSTCCRLTT-------GQWGCCPHVQAVCCDDHVHCCPAGYTCDLQAGTCLRTS-TLHTVAL 673 HSP 5 Score: 70.4774 bits (171), Expect = 9.115e-14 Identity = 53/165 (32.12%), Postives = 76/165 (46.06%), Query Frame = 1
Query: 124 VTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNE--NDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVEL 612
VT +++ N+ C TCC+TK + CC AVCC D HCCP+G+ C + CV ++ ++ L+ V+ +P N + NE + CP T CK + CCP AVCC D CCP T C++ C S+FS +L
Sbjct: 263 VTVESVPCNESVACESGSTCCKTKDGDWACCPLPEAVCCEDHIHCCPQGTVC--ARSTCV----ASADLLTLV--RWVEKKPTINRVPQPANEKCDPTTTCPKGATCCKMES-------GTWGCCPLPQAVCCEDHLHCCPRNTVCNVAAGTC-DSAFSGTAGQL 411 HSP 6 Score: 68.9366 bits (167), Expect = 2.995e-13 Identity = 46/147 (31.29%), Postives = 69/147 (46.94%), Query Frame = 1
Query: 148 NKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSS 588
+ +C TCC+ + CC + AVCC D HCCP G TCD + C++ + + L+ + ++VMC + + C +++TCC + CCP K AVCC D CCP G CD K K C +++
Sbjct: 612 DSTARCPTGSTCCRLTTGQWGCCPHVQAVCCDDHVHCCPAGYTCDLQAGTCLRTSTLHTVALTLV----------------HTHTKEQVMC-DATSHCTESQTCCKLSDTEWACCPFKQAVCCKDMKHCCPVGYVCDPKAKGCARAT 741 HSP 7 Score: 67.0106 bits (162), Expect = 1.406e-12 Identity = 50/161 (31.06%), Postives = 69/161 (42.86%), Query Frame = 1
Query: 148 NKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKN----NILEAKVNE-------NDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSV 597
++ + C TCC+ K ++ CC AVCC D HCCP+G CD C ++ K PAL+ + N I E+ V + + + CP + C + K N + CCP AVCC D CCP TCD CLK +
Sbjct: 432 DETSACPTGTTCCRQKSGAWACCPLPQAVCCDDHEHCCPQGYKCDIIHESCDHPFLPSMLWFKKQPALQSMTQQSNANAKAIAESAVADVESKHKCDAQTSCPRD------NTCCYMKKFNKWGCCPLPEAVCCGDGDHCCPNSYTCDTTKNTCLKGHLEI 586 HSP 8 Score: 48.521 bits (114), Expect = 2.101e-6 Identity = 25/69 (36.23%), Postives = 34/69 (49.28%), Query Frame = 1
Query: 100 VLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVK 306
V L T + + + + CT+ QTCC+ + CC + AVCC D HCCP G CD K C +
Sbjct: 671 VALTLVHTHTKEQVMCDATSHCTESQTCCKLSDTEWACCPFKQAVCCKDMKHCCPVGYVCDPKAKGCAR 739
BLAST of Granulin precursor vs. Ensembl Cavefish
Match: grnb (granulins-like [Source:NCBI gene;Acc:103046308]) HSP 1 Score: 111.309 bits (277), Expect = 8.712e-28 Identity = 65/172 (37.79%), Postives = 98/172 (56.98%), Query Frame = 1
Query: 79 DGVMSFDVLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALK-VDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSF 591
D VM + Q ++ +CP++ ++C D TCCQ S+ CC NAVCC DK HCCP+G+TCD KCV +V + + PA K + + + + + ++++V CP+ + C D TCC + +Y CCP AVCCSD CCP+GT+CD+ + KC+ S+
Sbjct: 79 DWVMRVPI---RQPSLPQAVVCPDQESECPDDTTCCQLPDGSWGCCPMPNAVCCEDKMHCCPQGTTCDLPHSKCVSSMLGSVPLRRKFPAYKRAEWKARKDSNGLDLADSNDVDCPDKVSFCPDDTTCCPLGNGSYGCCPMPKAVCCSDHVHCCPQGTSCDLAHSKCVSSNV 247 HSP 2 Score: 88.1965 bits (217), Expect = 8.348e-20 Identity = 58/152 (38.16%), Postives = 77/152 (50.66%), Query Frame = 1
Query: 139 ICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFS 594
ICP+ C D TCCQT Y CC NA CC D HCC EG+ CD + KCV ++ +P + ++ +A V + E CP++ TCC ++ CCP NAVCC DK CCP+GTTCD+ + KC+ S
Sbjct: 19 ICPDGGM-CDDGNTCCQTPSGGYGCCPLPNAECCSDHLHCCHEGTLCDLEHSKCVN-KTHTLDWVMRVPIR------QPSLPQAVVCPDQESECPDD-------TTCCQLPDGSWGCCPMPNAVCCEDKMHCCPQGTTCDLPHSKCVSSMLG 155 HSP 3 Score: 77.7962 bits (190), Expect = 2.824e-16 Identity = 54/141 (38.30%), Postives = 68/141 (48.23%), Query Frame = 1
Query: 133 DNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTC 555
D CP+K + C D TCC + SY CC AVCC D HCCP+G++CD KCV L P ++V + V N+ V C + TCC TK ++ CCP AVCC D CCP+GT C
Sbjct: 187 DVDCPDKVSFCPDDTTCCPLGNGSYGCCPMPKAVCCSDHVHCCPQGTSCDLAHSKCVSSNVLEPVTTNLSPVVRV------KVTVESVPCNESVACESG-------STCCKTKDGDWACCPLPEAVCCEDHIHCCPQGTVC 314 HSP 4 Score: 72.0182 bits (175), Expect = 2.667e-14 Identity = 60/170 (35.29%), Postives = 76/170 (44.71%), Query Frame = 1
Query: 148 NKRTKCTDRQTCC-QTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKL-IPALKVDDEPKNNILEAKVNENDEVM-------------CPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVEL 612
+ +T C TCC K N + CC AVCC D HCCP TCD ++ C+K G L IP K EAKV + EV CP T C+ T + CCPH AVCC D CCP G TCD++ CL++S +++ V L
Sbjct: 528 DAQTSCPRDNTCCYMKKFNKWGCCPLPEAVCCGDGDHCCPNSYTCDTTKNTCLK--------GHLEIPWFKKQ--------EAKVTQGSEVAHGVADVQCDSTARCPTGSTCCRLTT-------GQWGCCPHVQAVCCDDHVHCCPAGYTCDLQAGTCLRTS-TLHTVAL 673 HSP 5 Score: 70.4774 bits (171), Expect = 9.115e-14 Identity = 53/165 (32.12%), Postives = 76/165 (46.06%), Query Frame = 1
Query: 124 VTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNE--NDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVEL 612
VT +++ N+ C TCC+TK + CC AVCC D HCCP+G+ C + CV ++ ++ L+ V+ +P N + NE + CP T CK + CCP AVCC D CCP T C++ C S+FS +L
Sbjct: 263 VTVESVPCNESVACESGSTCCKTKDGDWACCPLPEAVCCEDHIHCCPQGTVC--ARSTCV----ASADLLTLV--RWVEKKPTINRVPQPANEKCDPTTTCPKGATCCKMES-------GTWGCCPLPQAVCCEDHLHCCPRNTVCNVAAGTC-DSAFSGTAGQL 411 HSP 6 Score: 68.9366 bits (167), Expect = 2.995e-13 Identity = 46/147 (31.29%), Postives = 69/147 (46.94%), Query Frame = 1
Query: 148 NKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSS 588
+ +C TCC+ + CC + AVCC D HCCP G TCD + C++ + + L+ + ++VMC + + C +++TCC + CCP K AVCC D CCP G CD K K C +++
Sbjct: 612 DSTARCPTGSTCCRLTTGQWGCCPHVQAVCCDDHVHCCPAGYTCDLQAGTCLRTSTLHTVALTLV----------------HTHTKEQVMC-DATSHCTESQTCCKLSDTEWACCPFKQAVCCKDMKHCCPVGYVCDPKAKGCARAT 741 HSP 7 Score: 67.0106 bits (162), Expect = 1.406e-12 Identity = 50/161 (31.06%), Postives = 69/161 (42.86%), Query Frame = 1
Query: 148 NKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKN----NILEAKVNE-------NDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSV 597
++ + C TCC+ K ++ CC AVCC D HCCP+G CD C ++ K PAL+ + N I E+ V + + + CP + C + K N + CCP AVCC D CCP TCD CLK +
Sbjct: 432 DETSACPTGTTCCRQKSGAWACCPLPQAVCCDDHEHCCPQGYKCDIIHESCDHPFLPSMLWFKKQPALQSMTQQSNANAKAIAESAVADVESKHKCDAQTSCPRD------NTCCYMKKFNKWGCCPLPEAVCCGDGDHCCPNSYTCDTTKNTCLKGHLEI 586 HSP 8 Score: 48.521 bits (114), Expect = 2.101e-6 Identity = 25/69 (36.23%), Postives = 34/69 (49.28%), Query Frame = 1
Query: 100 VLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVK 306
V L T + + + + CT+ QTCC+ + CC + AVCC D HCCP G CD K C +
Sbjct: 671 VALTLVHTHTKEQVMCDATSHCTESQTCCKLSDTEWACCPFKQAVCCKDMKHCCPVGYVCDPKAKGCAR 739
BLAST of Granulin precursor vs. Ensembl Cavefish
Match: ENSAMXT00000030959.1 (pep primary_assembly:Astyanax_mexicanus-2.0:23:26782099:26792241:-1 gene:ENSAMXG00000014237.2 transcript:ENSAMXT00000030959.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 80.1073 bits (196), Expect = 3.386e-17 Identity = 60/218 (27.52%), Postives = 98/218 (44.95%), Query Frame = 1
Query: 82 GVMSFDVLLENQKNV-------------TTDNICP----NKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKV----------DDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVELPNDAEENGNK*YIE 654
G+ +D+ + QK V +D+ P + C D TCC+T + + CC Y CC D HCCP G CD+ +C++ A S + PA+ + +++P ++E + + V C ++ C D TCC T + + CCP+ CC D CCP G CD + +C++ + S+ + P A NK Y++
Sbjct: 74 GLPWYDIFMPLQKAVEETEEKPSTSVVELSDDSSPMVHCDSSYTCPDGTTCCRTPYGRWACCPYNMGQCCRDGIHCCPHGYHCDSTSTRCMQGALS-IRSSSPQPAMLIQNKQKAVEETEEKPSATLVELSGDSSPMVHC-DSSYTCPDGTTCCRTPYGRWACCPYNMGQCCRDGIHCCPHGYHCDSTSTRCMQGALSIRSSS-PQPAMLIQNKQYLQ 288 HSP 2 Score: 79.337 bits (194), Expect = 5.571e-17 Identity = 55/177 (31.07%), Postives = 87/177 (49.15%), Query Frame = 1
Query: 88 MSFDVLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALK-----VDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNN 603
M ++L V++D CP+ T C D TCC T Y CC NAVCCP + +CCP+G CD + C K + + L+ +++P +++E + + V C ++ C D TCC T + + CCP+ CC D CCP G CD + +C++ + S+ +
Sbjct: 1 MIVVLVLLMAGLVSSDITCPDGHT-CPDNNTCCLTDQG-YACCPAPNAVCCPGQEYCCPQGYKCDPSSPQ--GCVKQGLPWYDIFMPLQKAVEETEEKPSTSVVELSDDSSPMVHC-DSSYTCPDGTTCCRTPYGRWACCPYNMGQCCRDGIHCCPHGYHCDSTSTRCMQGALSIRS 172 HSP 3 Score: 65.4698 bits (158), Expect = 3.820e-12 Identity = 51/161 (31.68%), Postives = 65/161 (40.37%), Query Frame = 1
Query: 163 CTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCA----KSNVEMGKLI----------PALKVDDEPK--NNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSV 597
C D TCC+T + + CC Y CC D HCCP G CD+ +C++ A S+ + LI PA V EP N + E + N CP T C+ + CCP+K CC D CC G CD C K V
Sbjct: 218 CPDGTTCCRTPYGRWACCPYNMGQCCRDGIHCCPHGYHCDSTSTRCMQGALSIRSSSPQPAMLIQNKQYLQEDQPAPLVPLEPALDNQVQEGVIRCNGRFYCPAEYTCCQAPT-------GEWGCCPYKLGQCCKDGKHCCEFGYNCDSAYSSCKKGYSRV 371
BLAST of Granulin precursor vs. Ensembl Cavefish
Match: ENSAMXT00000057729.1 (pep primary_assembly:Astyanax_mexicanus-2.0:23:26782099:26792241:-1 gene:ENSAMXG00000014237.2 transcript:ENSAMXT00000057729.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 80.1073 bits (196), Expect = 3.386e-17 Identity = 60/218 (27.52%), Postives = 98/218 (44.95%), Query Frame = 1
Query: 82 GVMSFDVLLENQKNV-------------TTDNICP----NKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKV----------DDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVELPNDAEENGNK*YIE 654
G+ +D+ + QK V +D+ P + C D TCC+T + + CC Y CC D HCCP G CD+ +C++ A S + PA+ + +++P ++E + + V C ++ C D TCC T + + CCP+ CC D CCP G CD + +C++ + S+ + P A NK Y++
Sbjct: 74 GLPWYDIFMPLQKAVEETEEKPSTSVVELSDDSSPMVHCDSSYTCPDGTTCCRTPYGRWACCPYNMGQCCRDGIHCCPHGYHCDSTSTRCMQGALS-IRSSSPQPAMLIQNKQKAVEETEEKPSATLVELSGDSSPMVHC-DSSYTCPDGTTCCRTPYGRWACCPYNMGQCCRDGIHCCPHGYHCDSTSTRCMQGALSIRSSS-PQPAMLIQNKQYLQ 288 HSP 2 Score: 79.337 bits (194), Expect = 5.571e-17 Identity = 55/177 (31.07%), Postives = 87/177 (49.15%), Query Frame = 1
Query: 88 MSFDVLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALK-----VDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNN 603
M ++L V++D CP+ T C D TCC T Y CC NAVCCP + +CCP+G CD + C K + + L+ +++P +++E + + V C ++ C D TCC T + + CCP+ CC D CCP G CD + +C++ + S+ +
Sbjct: 1 MIVVLVLLMAGLVSSDITCPDGHT-CPDNNTCCLTDQG-YACCPAPNAVCCPGQEYCCPQGYKCDPSSPQ--GCVKQGLPWYDIFMPLQKAVEETEEKPSTSVVELSDDSSPMVHC-DSSYTCPDGTTCCRTPYGRWACCPYNMGQCCRDGIHCCPHGYHCDSTSTRCMQGALSIRS 172 HSP 3 Score: 65.4698 bits (158), Expect = 3.820e-12 Identity = 51/161 (31.68%), Postives = 65/161 (40.37%), Query Frame = 1
Query: 163 CTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCA----KSNVEMGKLI----------PALKVDDEPK--NNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSV 597
C D TCC+T + + CC Y CC D HCCP G CD+ +C++ A S+ + LI PA V EP N + E + N CP T C+ + CCP+K CC D CC G CD C K V
Sbjct: 218 CPDGTTCCRTPYGRWACCPYNMGQCCRDGIHCCPHGYHCDSTSTRCMQGALSIRSSSPQPAMLIQNKQYLQEDQPAPLVPLEPALDNQVQEGVIRCNGRFYCPAEYTCCQAPT-------GEWGCCPYKLGQCCKDGKHCCEFGYNCDSAYSSCKKGYSRV 371
BLAST of Granulin precursor vs. Ensembl Cavefish
Match: ENSAMXT00000034465.1 (pep primary_assembly:Astyanax_mexicanus-2.0:23:26782099:26792241:-1 gene:ENSAMXG00000014237.2 transcript:ENSAMXT00000034465.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 79.337 bits (194), Expect = 4.719e-17 Identity = 60/218 (27.52%), Postives = 98/218 (44.95%), Query Frame = 1
Query: 82 GVMSFDVLLENQKNV-------------TTDNICP----NKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKV----------DDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVELPNDAEENGNK*YIE 654
G+ +D+ + QK V +D+ P + C D TCC+T + + CC Y CC D HCCP G CD+ +C++ A S + PA+ + +++P ++E + + V C ++ C D TCC T + + CCP+ CC D CCP G CD + +C++ + S+ + P A NK Y++
Sbjct: 74 GLPWYDIFMPLQKAVEETEEKPSTSVVELSDDSSPMVHCDSSYTCPDGTTCCRTPYGRWACCPYNMGQCCRDGIHCCPHGYHCDSTSTRCMQGALS-IRSSSPQPAMLIQNKQKAVEETEEKPSATVVELSDDSSPMVHC-DSSYTCPDGTTCCRTPYGRWACCPYNMGQCCRDGIHCCPHGYHCDSTSTRCMQGALSIRSSS-PQPAMLIQNKQYLQ 288 HSP 2 Score: 79.337 bits (194), Expect = 5.736e-17 Identity = 55/177 (31.07%), Postives = 87/177 (49.15%), Query Frame = 1
Query: 88 MSFDVLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALK-----VDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNN 603
M ++L V++D CP+ T C D TCC T Y CC NAVCCP + +CCP+G CD + C K + + L+ +++P +++E + + V C ++ C D TCC T + + CCP+ CC D CCP G CD + +C++ + S+ +
Sbjct: 1 MIVVLVLLMAGLVSSDITCPDGHT-CPDNNTCCLTDQG-YACCPAPNAVCCPGQEYCCPQGYKCDPSSPQ--GCVKQGLPWYDIFMPLQKAVEETEEKPSTSVVELSDDSSPMVHC-DSSYTCPDGTTCCRTPYGRWACCPYNMGQCCRDGIHCCPHGYHCDSTSTRCMQGALSIRS 172 HSP 3 Score: 65.4698 bits (158), Expect = 4.082e-12 Identity = 51/161 (31.68%), Postives = 65/161 (40.37%), Query Frame = 1
Query: 163 CTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCA----KSNVEMGKLI----------PALKVDDEPK--NNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSV 597
C D TCC+T + + CC Y CC D HCCP G CD+ +C++ A S+ + LI PA V EP N + E + N CP T C+ + CCP+K CC D CC G CD C K V
Sbjct: 218 CPDGTTCCRTPYGRWACCPYNMGQCCRDGIHCCPHGYHCDSTSTRCMQGALSIRSSSPQPAMLIQNKQYLQEDQPAPLVPLEPALDNQVQEGVIRCNGRFYCPAEYTCCQAPT-------GEWGCCPYKLGQCCKDGKHCCEFGYNCDSAYSSCKKGYSRV 371
BLAST of Granulin precursor vs. Ensembl Sea Lamprey
Match: ENSPMAT00000009302.1 (pep scaffold:Pmarinus_7.0:GL483232:1183:11161:1 gene:ENSPMAG00000008413.1 transcript:ENSPMAT00000009302.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 85.5001 bits (210), Expect = 1.449e-19 Identity = 53/150 (35.33%), Postives = 76/150 (50.67%), Query Frame = 1
Query: 148 NKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSV 597
+ ++ C D TCC+T + CC AVCC D HCCP+G+TC+ C + ++ PAL+V EP I + D+ CP+ TCC ++ CCP AVCC D CCPEGTTC++ ++C K + S+
Sbjct: 293 DDQSSCPDGNTCCKTPGGDWACCPLVKAVCCDDHEHCCPQGTTCNLPAQTCDQ-GTLSLPWSTKTPALRVAGEPNVVICD------DQSSCPDG-------NTCCKMPSGDWGCCPLPKAVCCDDHQHCCPEGTTCNLPVERCDKGTLSL 428 HSP 2 Score: 84.7297 bits (208), Expect = 2.268e-19 Identity = 55/150 (36.67%), Postives = 74/150 (49.33%), Query Frame = 1
Query: 148 NKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSV 597
+ + C D TCC+ + CC AVCC D HCCP+G+TC+ C K A S + PAL+V EP + V +D+V CP+ T CK ++ CCP AVCC D CCPEGTTC++ C + S+
Sbjct: 527 DDQFSCPDGDTCCKMPSGDWGCCPLVKAVCCDDHEHCCPQGTTCNLPAQTCDKGALS-LPWSTKTPALRVAGEPND------VKCDDQVSCPDQTTCCKTGT-------GDWGCCPLVKAVCCDDHEHCCPEGTTCNVPAGSCDQGQLSL 662 HSP 3 Score: 83.1889 bits (204), Expect = 9.105e-19 Identity = 52/150 (34.67%), Postives = 75/150 (50.00%), Query Frame = 1
Query: 148 NKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSV 597
+ ++ C D TCC+ + CC AVCC D HCCPEG+TC+ +C K ++ PAL+V EP I + D+ CP+ TCC ++ CCP AVCC D CCP+GTTC++ + C + + S+
Sbjct: 371 DDQSSCPDGNTCCKMPSGDWGCCPLPKAVCCDDHQHCCPEGTTCNLPVERCDK-GTLSLPWSTKTPALRVAGEPNVVICD------DQFSCPDG-------NTCCKLPSGDWGCCPLVKAVCCDDHEHCCPQGTTCNLPAQTCDQGTLSL 506 HSP 4 Score: 80.8777 bits (198), Expect = 5.341e-18 Identity = 52/145 (35.86%), Postives = 73/145 (50.34%), Query Frame = 1
Query: 163 CTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSV 597
C D TCC+T + CC AVCC D HCCP+G+TC+ C + ++ PAL+V EP I + D+ CP+ TCC T ++ CCP AVCC D CCP+GTTC++ + C + + S+
Sbjct: 220 CPDGNTCCKTPGGDWACCPLVKAVCCDDHEHCCPQGTTCNLPAQTCDQ-GTLSLPWSTKTPALRVAGEPNVVICD------DQSSCPDG-------NTCCKTPGGDWACCPLVKAVCCDDHEHCCPQGTTCNLPAQTCDQGTLSL 350 HSP 5 Score: 77.0258 bits (188), Expect = 1.095e-16 Identity = 48/152 (31.58%), Postives = 72/152 (47.37%), Query Frame = 1
Query: 142 CPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSV 597
CP+ T C D TCC Y CC +AVCC D HCCP+ + CD K KCV K ++ P+ + + + + + ++N + P+ + CCP AVCC DK CCPEGT C++ ++ C ++ S+
Sbjct: 61 CPDG-TSCGDDATCCPLASGQYGCCSLEDAVCCSDYLHCCPKNTMCDLKLGKCVH-GKLSIPWVSKYPSKQAASDVQCDAFTSCSDQNTCCILPS----------------GQWGCCPLPKAVCCDDKEHCCPEGTICNVTSQTCNHAALSL 194 HSP 6 Score: 72.0182 bits (175), Expect = 6.075e-15 Identity = 47/148 (31.76%), Postives = 64/148 (43.24%), Query Frame = 1
Query: 148 NKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPA---LKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLK 582
+ + C D+ TCC+T + CC AVCC D HCCPEG+TC+ C + + ++ PA L V + CP+ T C+ + CCP A CCSD CCP G TCD+ C+K
Sbjct: 605 DDQVSCPDQTTCCKTGTGDWGCCPLVKAVCCDDHEHCCPEGTTCNVPAGSCDQ-GQLSLPWSSKTPARTRLTALLPASAAARGGSVRCDSAYACPDGTTCCQLP-------QGQWGCCPLPLATCCSDFVHCCPSGYTCDLAQASCIK 744
BLAST of Granulin precursor vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001039.1 (pep scaffold:Pmarinus_7.0:GL484091:11570:24665:-1 gene:ENSPMAG00000000936.1 transcript:ENSPMAT00000001039.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 66.6254 bits (161), Expect = 3.743e-13 Identity = 55/163 (33.74%), Postives = 76/163 (46.63%), Query Frame = 1
Query: 163 CTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNV--------ELPNDAE 627
C QTCC+++ + CC A CC D HCCP G++ D K +CV A LIP LK K L V +N V C C+ +TCC ++ + CC + A CCSD CCP T C + + C +SS +++ V LP D+
Sbjct: 379 CRRWQTCCRSRRGMWGCCNGRRATCCSDGIHCCPSGASGDLKG-QCVNSA-------ALIPWLK-----KEAALSGPVGDN-LVHC-GGGQYCRRWQTCCRSRRGMWGCCNGRRATCCSDGIHCCPSMTRCLVPYRLCSRSSEALSGVAGSSTLSHALPYDSR 526 HSP 2 Score: 58.151 bits (139), Expect = 2.432e-10 Identity = 45/136 (33.09%), Postives = 61/136 (44.85%), Query Frame = 1
Query: 163 CTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNK 570
C QTCC+++ + CC A CC D HCCP + C Q V LIP LK K L V +N V+ C+ +TCC ++ + CC + A CCSD CCP G + D+K +
Sbjct: 303 CRRWQTCCRSRCGMWGCCNGRKATCCSDGIHCCPSMTRCLVGQ---------CVNSAALIPWLK-----KEAALSGPVGDN--VVHCGGGQYCRRWQTCCRSRRGMWGCCNGRRATCCSDGIHCCPSGASGDLKGQ 422 HSP 3 Score: 54.6842 bits (130), Expect = 4.587e-9 Identity = 42/133 (31.58%), Postives = 53/133 (39.85%), Query Frame = 1
Query: 163 CTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDI 561
C QTCC+++ + CC A CC D HCCP + C Q V LIP LK K L V +N V C + + C + CC + A CCSD CCP T C +
Sbjct: 151 CRRWQTCCRSRRGMWGCCNGRRATCCSDGIHCCPSMTRCLVGQ---------CVNSAALIPWLK-----KEAALSGPVGDN-VVHCGGGQYCRRWQTCCRSRC-GMWGCCNGRRATCCSDGIHCCPSMTRCLV 267 HSP 4 Score: 54.299 bits (129), Expect = 4.630e-9 Identity = 44/153 (28.76%), Postives = 68/153 (44.44%), Query Frame = 1
Query: 181 CCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAK-QHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVELPNDAEENG 636
CC+++ + CC A CC D HCCP + C + +CV A LIP LK K L V +N V C + + C + CC + A CCSD CCP T C + + C +SS +++ ++ + +++G
Sbjct: 1 CCRSRCGMWGCCNGRKATCCSDGIHCCPSMTRCLVPYRGQCVNSAA-------LIPWLK-----KEAALSGPVGDN-LVHCGGGQYCRRWQTCCRSRC-GMWGCCNGRKATCCSDGIHCCPSMTRCLVPYRLCSRSSEALSVLDYLSREQQDG 139 HSP 5 Score: 47.3654 bits (111), Expect = 1.059e-6 Identity = 33/136 (24.26%), Postives = 52/136 (38.24%), Query Frame = 1
Query: 163 CTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNE---NDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDI 561
C QTCC+++ + CC A CC D HCCP + C C + + E ++ L + + N++ + + C + + CC + A CCSD CCP T C +
Sbjct: 74 CRRWQTCCRSRCGMWGCCNGRKATCCSDGIHCCPSMTRCLVPYRLCSR----SSEALSVLDYLSREQQDGYNVVHCGGGQYCRRWQTCCRSR--------------RGMWGCCNGRRATCCSDGIHCCPSMTRCLV 191
BLAST of Granulin precursor vs. Ensembl Nematostella
Match: EDO27824 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T870]) HSP 1 Score: 62.7734 bits (151), Expect = 3.852e-12 Identity = 49/146 (33.56%), Postives = 65/146 (44.52%), Query Frame = 1
Query: 148 NKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKS 585
N + C D +TCC+ SY CC NAVCC D HCCP G CD C AK + M ++ + + E K + +TCC+ Y CCP +AVCC+D CCP G TC+ K+ C +
Sbjct: 10 NDGSHCYDYETCCELSTGSYGCCPLPNAVCCSDGKHCCPCGYHCDISSSTCDM-AKDSTPMVSYHATVQ-----RAGVGEIKCKDGSHC---------NTGETCCLVGPTKYGCCPLPSAVCCADMQHCCPSGYTCNDKSGHCQRG 140 HSP 2 Score: 55.8398 bits (133), Expect = 1.221e-9 Identity = 44/147 (29.93%), Postives = 62/147 (42.18%), Query Frame = 1
Query: 157 TKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEV-MCPNNXXXXXXXXXXX----XXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLK 582
+ C +TCC Y CC +AVCC D HCCP G TC+ K C + + + M + P++ P+ + + V + + P + CCP NA CCSD CCP+G CD K C+K
Sbjct: 90 SHCNTGETCCLVGPTKYGCCPLPSAVCCADMQHCCPSGYTCNDKSGHCQR-GRHVLPMFQKHPSV----HPRPAVAKTPVIIDHTIRFVPRGGRGAGCACEPYHTCCGSSSAQQNCCPLPNASCCSDGLHCCPQGFICD-DQKGCVK 230
BLAST of Granulin precursor vs. Ensembl Medaka
Match: grnb (granulin precursor [Source:NCBI gene;Acc:101166910]) HSP 1 Score: 102.064 bits (253), Expect = 1.251e-24 Identity = 67/212 (31.60%), Postives = 97/212 (45.75%), Query Frame = 1
Query: 25 VAILGFNLLLIFV-VKIYADGVMSFDVLLENQKNVT--------TDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVELPNDAEEN 633
V ++G + L+ + ADG F L + T ICP++ ++C D TCCQ S+ CC + AVCC D+ HCCPEG+TCD KC + M + +PA +V P + V + CPN+ T C T ++ CCP+ A CCSD CCP TTCD++ C V + + + E+
Sbjct: 6 VVLVGLSAALLCPDGAVCADGHKCF---LNSHGGFTCPLPHTRVGAVICPDQESECPDETTCCQLPDGSWGCCPFPKAVCCEDQRHCCPEGTTCDLVHSKCASPSLQAFPMLQKVPAQRVGTLPVFAAAASVVCPGGKSSCPNSFTCCLLTS-------GDFGCCPYPQATCCSDHLHCCPGNTTCDLERGVCKSGEARVEPIRAVSASPED 207 HSP 2 Score: 93.2041 bits (230), Expect = 1.312e-21 Identity = 63/180 (35.00%), Postives = 83/180 (46.11%), Query Frame = 1
Query: 91 SFDVLLENQKNVTT---DNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEA-KVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVELPN 618
S + +E + V+ D +CP++R+ C D TCCQ + ++ CC NAVCC D HCCPEG+TCD CV A E ++P PK + + V N V C + TCC + CCP AVCC D CCP GT C++ C S S L N
Sbjct: 190 SGEARVEPIRAVSASPEDVLCPDQRSMCPDGTTCCQLNNGTFGCCPLPNAVCCSDHMHCCPEGTTCDLAHSTCVSAAGGTAEA-VMVPVSGT--PPKTHPVRGLAVPCNASVACADG-------STCCRASRGQWGCCPLPEAVCCEDHLHCCPHGTVCNLVASTCDDPSGSALTPLLSN 359 HSP 3 Score: 88.9669 bits (219), Expect = 4.843e-20 Identity = 59/185 (31.89%), Postives = 86/185 (46.49%), Query Frame = 1
Query: 58 FVVKIYADGVMSFDVLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVEL 612
+ K+ A V + V +CP ++ C + TCC + CC Y A CC D HCCP +TCD ++ C K ++ VE P V P ++V+CP+ ++ C D TCC + + CCP NAVCCSD CCPEGTTCD+ + C+ ++ +
Sbjct: 113 MLQKVPAQRVGTLPVFA-----AAASVVCPGGKSSCPNSFTCCLLTSGDFGCCPYPQATCCSDHLHCCPGNTTCDLERGVC-KSGEARVE-----PIRAVSASP------------EDVLCPDQRSMCPDGTTCCQLNNGTFGCCPLPNAVCCSDHMHCCPEGTTCDLAHSTCVSAAGGTAEAVM 274 HSP 4 Score: 75.8702 bits (185), Expect = 1.263e-15 Identity = 51/166 (30.72%), Postives = 75/166 (45.18%), Query Frame = 1
Query: 112 NQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKC-LKSSFSVNNV 606
+ + TD C NK + C TCC + + CC AVCC D HCCP+ TC+ + C K A + + +++PA D + + + E CP T C+ + + CCP AVCC D CCP G +CD+K C L S +++
Sbjct: 602 TEPSGVTDVKCDNK-SSCASGTTCCPLQTGGWGCCPLVKAVCCADHEHCCPQSYTCNMQTGTCEKKAGQGLPLSRVVPAQPQDTQ------DVPCDGTGEFKCPKQNTCCRISA-------TAWGCCPSPGAVCCMDSKHCCPAGYSCDLKAGGCTLGPRLSWDSL 753 HSP 5 Score: 66.2402 bits (160), Expect = 2.425e-12 Identity = 47/150 (31.33%), Postives = 66/150 (44.00%), Query Frame = 1
Query: 148 NKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSV 597
K C R TCC+ + CC AVCC D HCCP+G C+A C + ++ + +PAL+ + +E K + + CP + T C +T + CCP AVCC D CCP G +C C + S V
Sbjct: 452 GKDAVCPGRTTCCKNSSGQWACCPLPQAVCCSDLDHCCPKGYKCNAFDRTCERPGGPSLPWRQKLPALQQRSG-QTGPVENKCDA--QTSCPKDTTCCFMEQTL------KWGCCPLPQAVCCGDGNHCCPRGHSCQPHRSSCSRGSHVV 592 HSP 6 Score: 62.003 bits (149), Expect = 7.559e-11 Identity = 46/171 (26.90%), Postives = 72/171 (42.11%), Query Frame = 1
Query: 148 NKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSV--NNVELPNDAEENGNK*YIE 654
++ C TCC+T+ + CC AVCC D HCCP + C+ + C + +P L + E K + + +CP T CK++ + CCP AVCCSD CCP+G C+ ++ C + +LP + +G +E
Sbjct: 373 DESASCPGEATCCRTRAGGWACCPLPQAVCCDDHIHCCPNATICNLEAETC----DDPSGLSPPLPWLTKRPRLTAGMEEEKCGK--DAVCPGRTTCCKNSS-------GQWACCPLPQAVCCSDLDHCCPKGYKCNAFDRTCERPGGPSLPWRQKLPALQQRSGQTGPVE 530 HSP 7 Score: 53.1434 bits (126), Expect = 7.881e-8 Identity = 46/146 (31.51%), Postives = 64/146 (43.84%), Query Frame = 1
Query: 148 NKRTKCTDRQTCCQTKHN-SYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLK 582
+ +T C TCC + + CC AVCC D HCCP G +C + C + ++P K L D V C +NK+ C TCC + + CCP AVCC+D CCP+ TC+++ C K
Sbjct: 534 DAQTSCPKDTTCCFMEQTLKWGCCPLPQAVCCGDGNHCCPRGHSCQPHRSSCSR-------GSHVVPWFT-----KVGALTEPSGVTD-VKC-DNKSSCASGTTCCPLQTGGWGCCPLVKAVCCADHEHCCPQSYTCNMQTGTCEK 665
BLAST of Granulin precursor vs. Ensembl Medaka
Match: grna (granulins [Source:NCBI gene;Acc:101162601]) HSP 1 Score: 83.1889 bits (204), Expect = 4.345e-18 Identity = 60/171 (35.09%), Postives = 81/171 (47.37%), Query Frame = 1
Query: 85 VMSFDVLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSV 597
V SF + ++V D T C D+ TCC+T+ + CC + AVCC D HCCP+G TC+ C +V M + +P+ K ++ V + CP+ TCC TK + CCP AVCC D CCP GTTCD+ CLK+S V
Sbjct: 676 VPSFSNVSVKMEDVPCDG-----STSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEG-DGGSVPMLEKVPSFS-----KVSVKMEDVPCDGSTSCPDQ-------TTCCKTKDGAWGCCPLPEAVCCDDHEHCCPTGTTCDLSTLSCLKASGLV 828 HSP 2 Score: 80.1073 bits (196), Expect = 5.715e-17 Identity = 61/201 (30.35%), Postives = 90/201 (44.78%), Query Frame = 1
Query: 7 SKMKNFVAILGFNLLLIFVVKIYADGVMSFDVLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVE 609
K + VA+ G N F A+ V +F + +I N+ C D+ TCC+T+ + CC + AVCC D HCCP+G TC+ C +V M + +P+ K ++ V + CP+ TCC T+ + CCP AVCC D CCP+G TC++ + C SV +E
Sbjct: 251 GKEVSAVAVEG-NETKNFPEVTTANPVPAFLEGSSVAGEASAKDIPCNETASCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEG-DGGSVPMLEKVPSFS-----KVSVKMEDVPCDGSTSCPDQ-------TTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSVPMLE 437 HSP 3 Score: 78.9518 bits (193), Expect = 1.132e-16 Identity = 56/175 (32.00%), Postives = 81/175 (46.29%), Query Frame = 1
Query: 85 VMSFDVLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVE 609
V SF + ++V D T C D+ TCC+T+ + CC + AVCC D HCCP+G TC+ C +V M + +P+ K ++ V + CP+ TCC T+ + CCP AVCC D CCP+G TC++ + C SV +E
Sbjct: 518 VPSFSNVSVKMEDVPCDG-----STSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEG-DGGSVPMLEKVPSFS-----KVSVKMEDVPCDGSTSCPDQ-------TTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSVPMLE 674 HSP 4 Score: 77.7962 bits (190), Expect = 3.405e-16 Identity = 52/143 (36.36%), Postives = 71/143 (49.65%), Query Frame = 1
Query: 160 KCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSS 588
+C + +CC+ + CC NAVCC DK HCCPEG TCD C K +E L P DD+ ++ ++ +N D+V N +TCC T H + CC AVCC D CCP+G C K C+K++
Sbjct: 969 QCPEHMSCCRLFTGEWGCCPLPNAVCCGDKEHCCPEGYTCDLASKSCHKLLTLQLETVPLTPVFLPDDQSQHGPVKPILNRCDDVYSCN------VDETCCRTSHTTWGCCLSPKAVCCRDMKHCCPDGYRC-TKGGSCIKNT 1104 HSP 5 Score: 77.411 bits (189), Expect = 3.926e-16 Identity = 55/175 (31.43%), Postives = 80/175 (45.71%), Query Frame = 1
Query: 85 VMSFDVLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVE 609
V SF + ++V D T C D+ TCC+T+ + CC + AVCC D HCCP+G TC+ C +V M + +P+ ++ V + CP+ TCC T+ + CCP AVCC D CCP+G TC++ + C SV +E
Sbjct: 360 VPSFSKVSVKMEDVPCDG-----STSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEG-DGGSVPMLEKVPSFS-----NVSVKMEDVPCDGSTSCPDQ-------TTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSVPMLE 516 HSP 6 Score: 77.411 bits (189), Expect = 3.926e-16 Identity = 54/175 (30.86%), Postives = 78/175 (44.57%), Query Frame = 1
Query: 85 VMSFDVLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVE 609
V SF + ++V D T C D+ TCC+T+ + CC + AVCC D HCCP+G TC+ C +V M + +P+ ++ V + CP+ T CK + CCP AVCC D CCP+G TC++ + C SV +E
Sbjct: 597 VPSFSKVSVKMEDVPCDG-----STSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEG-DGGSVPMLEKVPSFS-----NVSVKMEDVPCDGSTSCPDQTTCCKTQ-------EGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSVPMLE 753 HSP 7 Score: 77.411 bits (189), Expect = 4.657e-16 Identity = 59/190 (31.05%), Postives = 83/190 (43.68%), Query Frame = 1
Query: 139 ICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEM-----------------GKLIPALKVDDEPKNNILE----------------AKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVE 609
+C + ++C D TCC+ + + CC AVCC DK HCCPE +TCD +Q KC+ + + M GK + A+ V+ N E A ++ C N C D TCC T+ + CCP AVCC D CCP+G TC++ + C SV +E
Sbjct: 170 LCKDGVSECPDETTCCENQEGKWACCPMPKAVCCDDKIHCCPEETTCDVEQMKCLSSSDKALPMWAKFPARTRADWENQKEGKEVSAVAVEGNETKNFPEVTTANPVPAFLEGSSVAGEASAKDIPC-NETASCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSVPMLE 358 HSP 8 Score: 76.2554 bits (186), Expect = 1.214e-15 Identity = 59/185 (31.89%), Postives = 83/185 (44.86%), Query Frame = 1
Query: 43 NLLLIFVVKIYADGVMSFDV--LLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNE-NDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSS 588
L + +V ++ A+ M+ ++ + DNICP+ +++C +C + Y CC A V CPD HCCPEG C CVK K ++ K + + V+E DE C N+ + CCP AVCC DK CCPE TTCD++ KCL SS
Sbjct: 75 TLSMRWVERVSANLQMATKSFRMIRSYMGEDEDNICPD-QSRCPAEFSCLKA-LTRYGCCPLAQGVSCPDGKHCCPEGHQCSLDSRSCVK--KESI---------------KTVLCKDGVSECPDETTCCENQ-------------EGKWACCPMPKAVCCDDKIHCCPEETTCDVEQMKCLSSS 227 HSP 9 Score: 73.9442 bits (180), Expect = 6.191e-15 Identity = 56/195 (28.72%), Postives = 82/195 (42.05%), Query Frame = 1
Query: 85 VMSFDVLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNIL------------------------EAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSV 597
V SF + ++V D T C D+ TCC+TK ++ CC AVCC D HCCP G+TCD C+K A V M K +PA + ++ E K +E + C ++ + T C + + + CCP AVCC+D CCP C ++ C+K +
Sbjct: 755 VPSFSKVSVKMEDVPCDG-----STSCPDQTTCCKTKDGAWGCCPLPEAVCCDDHEHCCPTGTTCDLSTLSCLK-ASGLVPMSKKLPASTRTSSATSQLMKTGQETEEGENKPDDRDGQEDFDEEDKTDEEGIISCDSHTQCPQHTTCCFMKQVQKWGCCPLPKAVCCADGEHCCPADYKCKEEDTSCVKGEVVI 943 HSP 10 Score: 72.0182 bits (175), Expect = 2.658e-14 Identity = 45/166 (27.11%), Postives = 74/166 (44.58%), Query Frame = 1
Query: 85 VMSFDVLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLK 582
+ + V+ + ++CPN KC QTCCQ+ Y CC + A CC D HCCP+ CD C S + ++ L++ + ++ + + E+++ +CP+ + Y CCP V C D CCPEG C + ++ C+K
Sbjct: 1 MQKWVVICWVSLALVAADMCPNGG-KCEKGQTCCQSPSKDYDCCPFDEAECCGDHIHCCPKDMLCDMTTSSCGNATLSMRWVERVSANLQMATK-SFRMIRSYMGEDEDNICPDQSRCPAEFSCLKAL--TRYGCCPLAQGVSCPDGKHCCPEGHQCSLDSRSCVK 162 HSP 11 Score: 56.6102 bits (135), Expect = 4.381e-9 Identity = 60/199 (30.15%), Postives = 80/199 (40.20%), Query Frame = 1
Query: 79 DGVMSFDVLLENQKNVTTDNICPNKRTKCTDRQTCCQTKH-NSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKC----VKCAKSNVEMGKL--IPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLK------SSFSVNNVELPNDAEENG 636
DG FD E K I + T+C TCC K + CC AVCC +G C +KC C K V + IPA + + + V++ E M +CC + CCP NAVCC DK CCPEG TCD+ +K C K + + V LP+D ++G
Sbjct: 865 DGQEDFD---EEDKTDEEGIISCDSHTQCPQHTTCCFMKQVQKWGCCPLPKAVCCA-------DGEHCCPADYKCKEEDTSCVKGEVVIPWYTKIPATDSIQADSSFVQCSAVHQCPEHM------------SCCRLFTGEWGCCPLPNAVCCGDKEHCCPEGYTCDLASKSCHKLLTLQLETVPLTPVFLPDDQSQHG 1041
BLAST of Granulin precursor vs. Ensembl Medaka
Match: grna (granulins [Source:NCBI gene;Acc:101162601]) HSP 1 Score: 83.1889 bits (204), Expect = 4.953e-18 Identity = 58/171 (33.92%), Postives = 79/171 (46.20%), Query Frame = 1
Query: 85 VMSFDVLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSV 597
V SF + ++V D T C D+ TCC+T+ + CC + AVCC D HCCP+G TC+ C +V M + +P+ K ++ V + CP+ T CK + CCP AVCC D CCP GTTCD+ CLK+S V
Sbjct: 597 VPSFSNVSVKMEDVPCDG-----STSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEG-DGGSVPMLEKVPSFS-----KVSVKMEDVPCDGSTSCPDQTTCCKTK-------DGAWGCCPLPEAVCCDDHEHCCPTGTTCDLSTLSCLKASGLV 749 HSP 2 Score: 79.7221 bits (195), Expect = 6.638e-17 Identity = 61/201 (30.35%), Postives = 90/201 (44.78%), Query Frame = 1
Query: 7 SKMKNFVAILGFNLLLIFVVKIYADGVMSFDVLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVE 609
K + VA+ G N F A+ V +F + +I N+ C D+ TCC+T+ + CC + AVCC D HCCP+G TC+ C +V M + +P+ K ++ V + CP+ TCC T+ + CCP AVCC D CCP+G TC++ + C SV +E
Sbjct: 251 GKEVSAVAVEG-NETKNFPEVTTANPVPAFLEGSSVAGEASAKDIPCNETASCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEG-DGGSVPMLEKVPSFS-----KVSVKMEDVPCDGSTSCPDQ-------TTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSVPMLE 437 HSP 3 Score: 77.7962 bits (190), Expect = 3.271e-16 Identity = 52/143 (36.36%), Postives = 71/143 (49.65%), Query Frame = 1
Query: 160 KCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSS 588
+C + +CC+ + CC NAVCC DK HCCPEG TCD C K +E L P DD+ ++ ++ +N D+V N +TCC T H + CC AVCC D CCP+G C K C+K++
Sbjct: 890 QCPEHMSCCRLFTGEWGCCPLPNAVCCGDKEHCCPEGYTCDLASKSCHKLLTLQLETVPLTPVFLPDDQSQHGPVKPILNRCDDVYSCN------VDETCCRTSHTTWGCCLSPKAVCCRDMKHCCPDGYRC-TKGGSCIKNT 1025 HSP 4 Score: 77.411 bits (189), Expect = 4.560e-16 Identity = 55/175 (31.43%), Postives = 80/175 (45.71%), Query Frame = 1
Query: 85 VMSFDVLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVE 609
V SF + ++V D T C D+ TCC+T+ + CC + AVCC D HCCP+G TC+ C +V M + +P+ ++ V + CP+ TCC T+ + CCP AVCC D CCP+G TC++ + C SV +E
Sbjct: 360 VPSFSKVSVKMEDVPCDG-----STSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEG-DGGSVPMLEKVPSFS-----NVSVKMEDVPCDGSTSCPDQ-------TTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSVPMLE 516 HSP 5 Score: 77.411 bits (189), Expect = 4.966e-16 Identity = 59/190 (31.05%), Postives = 83/190 (43.68%), Query Frame = 1
Query: 139 ICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEM-----------------GKLIPALKVDDEPKNNILE----------------AKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVE 609
+C + ++C D TCC+ + + CC AVCC DK HCCPE +TCD +Q KC+ + + M GK + A+ V+ N E A ++ C N C D TCC T+ + CCP AVCC D CCP+G TC++ + C SV +E
Sbjct: 170 LCKDGVSECPDETTCCENQEGKWACCPMPKAVCCDDKIHCCPEETTCDVEQMKCLSSSDKALPMWAKFPARTRADWENQKEGKEVSAVAVEGNETKNFPEVTTANPVPAFLEGSSVAGEASAKDIPC-NETASCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSVPMLE 358 HSP 6 Score: 77.0258 bits (188), Expect = 6.236e-16 Identity = 55/175 (31.43%), Postives = 80/175 (45.71%), Query Frame = 1
Query: 85 VMSFDVLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVE 609
V SF + ++V D T C D+ TCC+T+ + CC + AVCC D HCCP+G TC+ C +V M + +P+ ++ V + CP+ TCC T+ + CCP AVCC D CCP+G TC++ + C SV +E
Sbjct: 518 VPSFSNVSVKMEDVPCDG-----STSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEG-DGGSVPMLEKVPSFS-----NVSVKMEDVPCDGSTSCPDQ-------TTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSVPMLE 674 HSP 7 Score: 75.8702 bits (185), Expect = 1.319e-15 Identity = 59/185 (31.89%), Postives = 83/185 (44.86%), Query Frame = 1
Query: 43 NLLLIFVVKIYADGVMSFDV--LLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNE-NDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSS 588
L + +V ++ A+ M+ ++ + DNICP+ +++C +C + Y CC A V CPD HCCPEG C CVK K ++ K + + V+E DE C N+ + CCP AVCC DK CCPE TTCD++ KCL SS
Sbjct: 75 TLSMRWVERVSANLQMATKSFRMIRSYMGEDEDNICPD-QSRCPAEFSCLKA-LTRYGCCPLAQGVSCPDGKHCCPEGHQCSLDSRSCVK--KESI---------------KTVLCKDGVSECPDETTCCENQ-------------EGKWACCPMPKAVCCDDKIHCCPEETTCDVEQMKCLSSS 227 HSP 8 Score: 73.9442 bits (180), Expect = 6.356e-15 Identity = 56/195 (28.72%), Postives = 82/195 (42.05%), Query Frame = 1
Query: 85 VMSFDVLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNIL------------------------EAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSV 597
V SF + ++V D T C D+ TCC+TK ++ CC AVCC D HCCP G+TCD C+K A V M K +PA + ++ E K +E + C ++ + T C + + + CCP AVCC+D CCP C ++ C+K +
Sbjct: 676 VPSFSKVSVKMEDVPCDG-----STSCPDQTTCCKTKDGAWGCCPLPEAVCCDDHEHCCPTGTTCDLSTLSCLK-ASGLVPMSKKLPASTRTSSATSQLMKTGQETEEGENKPDDRDGQEDFDEEDKTDEEGIISCDSHTQCPQHTTCCFMKQVQKWGCCPLPKAVCCADGEHCCPADYKCKEEDTSCVKGEVVI 864 HSP 9 Score: 72.0182 bits (175), Expect = 2.943e-14 Identity = 45/166 (27.11%), Postives = 74/166 (44.58%), Query Frame = 1
Query: 85 VMSFDVLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLK 582
+ + V+ + ++CPN KC QTCCQ+ Y CC + A CC D HCCP+ CD C S + ++ L++ + ++ + + E+++ +CP+ + Y CCP V C D CCPEG C + ++ C+K
Sbjct: 1 MQKWVVICWVSLALVAADMCPNGG-KCEKGQTCCQSPSKDYDCCPFDEAECCGDHIHCCPKDMLCDMTTSSCGNATLSMRWVERVSANLQMATK-SFRMIRSYMGEDEDNICPDQSRCPAEFSCLKAL--TRYGCCPLAQGVSCPDGKHCCPEGHQCSLDSRSCVK 162 HSP 10 Score: 56.6102 bits (135), Expect = 4.376e-9 Identity = 60/199 (30.15%), Postives = 80/199 (40.20%), Query Frame = 1
Query: 79 DGVMSFDVLLENQKNVTTDNICPNKRTKCTDRQTCCQTKH-NSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKC----VKCAKSNVEMGKL--IPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLK------SSFSVNNVELPNDAEENG 636
DG FD E K I + T+C TCC K + CC AVCC +G C +KC C K V + IPA + + + V++ E M +CC + CCP NAVCC DK CCPEG TCD+ +K C K + + V LP+D ++G
Sbjct: 786 DGQEDFD---EEDKTDEEGIISCDSHTQCPQHTTCCFMKQVQKWGCCPLPKAVCCA-------DGEHCCPADYKCKEEDTSCVKGEVVIPWYTKIPATDSIQADSSFVQCSAVHQCPEHM------------SCCRLFTGEWGCCPLPNAVCCGDKEHCCPEGYTCDLASKSCHKLLTLQLETVPLTPVFLPDDQSQHG 962
BLAST of Granulin precursor vs. Ensembl Medaka
Match: ENSORLT00000005071.2 (granulins [Source:NCBI gene;Acc:101168633]) HSP 1 Score: 71.633 bits (174), Expect = 1.613e-14 Identity = 54/169 (31.95%), Postives = 72/169 (42.60%), Query Frame = 1
Query: 142 CPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLI-------PALKVDDEPKNNILEAKVNENDEV------------MCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSF 591
CP+ + C D TCC T H + CC AVCC DK HCCP G C+ C K + V + L PAL + N++E N+N MCP+ TCC ++CCP+ CC D + CCP G CD+ C++ F
Sbjct: 26 CPDGKL-CPDLSTCCLTGHG-FSCCPLPEAVCCSDKAHCCPSGYRCNLATMMCEKTDQPWVSLPMLTKEAAEEPPALDL----STNLVEELKNDNGLAQKSTGVQCDSFYMCPDR-------TTCCRHPKGAWFCCPYYPGRCCLDGFHCCPYGYDCDLTYTHCIRDGF 181 HSP 2 Score: 65.4698 bits (158), Expect = 2.680e-12 Identity = 58/206 (28.16%), Postives = 85/206 (41.26%), Query Frame = 1
Query: 52 LIFVVKIYADGVMSFDVLLENQKNVTTDNICPNKRTK--------CTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKL----IPALKVDDEPKNNI-LEAKV----------NENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVN 600
L + K A+ + D+ + + DN K T C DR TCC+ ++ CC Y CC D FHCCP G CD C++ K +PA + +N+I LE + +E+ + C ++K C +CC + CCP+ CC+D CC G TCD + C K S+ V
Sbjct: 86 LPMLTKEAAEEPPALDLSTNLVEELKNDNGLAQKSTGVQCDSFYMCPDRTTCCRHPKGAWFCCPYYPGRCCLDGFHCCPYGYDCDLTYTHCIRDGFKYPFYYKQALTSVPAALISHPEENSIQLEKSLTALTEASDAPSEHGVIRC-DSKFFCPAGTSCCKGAEGQWTCCPYPLGKCCADGRHCCQYGYTCDATSATCRKLSYPVG 290
BLAST of Granulin precursor vs. Ensembl Medaka
Match: ENSORLT00000029765.1 (granulins [Source:NCBI gene;Acc:101168633]) HSP 1 Score: 71.2478 bits (173), Expect = 1.750e-14 Identity = 54/169 (31.95%), Postives = 72/169 (42.60%), Query Frame = 1
Query: 142 CPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLI-------PALKVDDEPKNNILEAKVNENDEV------------MCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSF 591
CP+ + C D TCC T H + CC AVCC DK HCCP G C+ C K + V + L PAL + N++E N+N MCP+ TCC ++CCP+ CC D + CCP G CD+ C++ F
Sbjct: 24 CPDGKL-CPDLSTCCLTGHG-FSCCPLPEAVCCSDKAHCCPSGYRCNLATMMCEKTDQPWVSLPMLTKEAAEEPPALDL----STNLVEELKNDNGLAQKSTGVQCDSFYMCPDR-------TTCCRHPKGAWFCCPYYPGRCCLDGFHCCPYGYDCDLTYTHCIRDGF 179 HSP 2 Score: 61.2326 bits (147), Expect = 7.138e-11 Identity = 56/200 (28.00%), Postives = 82/200 (41.00%), Query Frame = 1
Query: 52 LIFVVKIYADGVMSFDVLLENQKNVTTDNICPNKRTK--------CTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKL----IPALKVDDEPKNNI-LEAKV----------NENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLK 582
L + K A+ + D+ + + DN K T C DR TCC+ ++ CC Y CC D FHCCP G CD C++ K +PA + +N+I LE + +E+ + C ++K C +CC + CCP+ CC+D CC G TCD + C K
Sbjct: 84 LPMLTKEAAEEPPALDLSTNLVEELKNDNGLAQKSTGVQCDSFYMCPDRTTCCRHPKGAWFCCPYYPGRCCLDGFHCCPYGYDCDLTYTHCIRDGFKYPFYYKQALTSVPAALISHPEENSIQLEKSLTALTEASDAPSEHGVIRC-DSKFFCPAGTSCCKGAEGQWTCCPYPLGKCCADGRHCCQYGYTCDATSATCRK 282
BLAST of Granulin precursor vs. Planmine SMEST
Match: SMESG000018766.1 (SMESG000018766.1) HSP 1 Score: 291.197 bits (744), Expect = 2.063e-99 Identity = 160/185 (86.49%), Postives = 165/185 (89.19%), Query Frame = 1
Query: 88 MSFDVLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVELPNDAEENGNK 642
M V+ E + TD CPN RTKC D QTCC TKH SYHCCK+ANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEM KLIPALKV+DEPK NILEAKVNE DEVMCPNNKTKCKD KTCCITKHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFS+NNVELPNDAEENGNK
Sbjct: 116 MEMPVIEETDVKIITDQTCPNNRTKCQDSQTCCVTKHGSYHCCKFANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMRKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNKTKCKDIKTCCITKHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSLNNVELPNDAEENGNK 300 HSP 2 Score: 172.17 bits (435), Expect = 1.212e-52 Identity = 108/204 (52.94%), Postives = 139/204 (68.14%), Query Frame = 1
Query: 13 MKNFVAILGFNLLLIFVVKIY-ADGVMSFDV-LLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNN--ILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVEL 612
MKN +I+ ++L V + A G +SF++ L+N+ T NICPNKRTKCT+RQTCCQTKHNSYHCCKYANAVCCPDKFHCCP G++CD+ KC + N+E K ++ EP I E V + CPNN+TKC+D++TCC+TKH +Y+CC NAVCC DK+ CCPEG+TCD K KC+K + S NVE+
Sbjct: 1 MKNIFSIVTICVVLTLVSSLAEAGGTLSFNLSWLDNENKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPNGTSCDSVHKKCNR----NIEATK--SPIQTTPEPMEMPVIEETDVKIITDQTCPNNRTKCQDSQTCCVTKHGSYHCCKFANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKS--NVEM 196
BLAST of Granulin precursor vs. Planmine SMEST
Match: SMESG000018766.1 (SMESG000018766.1) HSP 1 Score: 289.656 bits (740), Expect = 2.856e-99 Identity = 160/185 (86.49%), Postives = 165/185 (89.19%), Query Frame = 1
Query: 88 MSFDVLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVELPNDAEENGNK 642
M V+ E + TD CPN RTKC D QTCC TKH SYHCCK+ANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEM KLIPALKV+DEPK NILEAKVNE DEVMCPNNKTKCKD KTCCITKHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFS+NNVELPNDAEENGNK
Sbjct: 82 MEMPVIEETDVKIITDQTCPNNRTKCQDSQTCCVTKHGSYHCCKFANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMRKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNKTKCKDIKTCCITKHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSLNNVELPNDAEENGNK 266 HSP 2 Score: 153.68 bits (387), Expect = 6.762e-46 Identity = 99/172 (57.56%), Postives = 123/172 (71.51%), Query Frame = 1
Query: 103 LLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNN--ILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVEL 612
+LEN++ T NICPNKRTKCT+RQTCCQTKHNSYHCCKYANAVCCPDKFHCCP G++CD+ KC + N+E K ++ EP I E V + CPNN+TKC+D++TCC+TKH +Y+CC NAVCC DK+ CCPEG+TCD K KC+K + S NVE+
Sbjct: 1 MLENKE--TVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPNGTSCDSVHKKCNR----NIEATK--SPIQTTPEPMEMPVIEETDVKIITDQTCPNNRTKCQDSQTCCVTKHGSYHCCKFANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKS--NVEM 162
BLAST of Granulin precursor vs. Planmine SMEST
Match: SMESG000018766.1 (SMESG000018766.1) HSP 1 Score: 289.271 bits (739), Expect = 7.452e-99 Identity = 160/185 (86.49%), Postives = 165/185 (89.19%), Query Frame = 1
Query: 88 MSFDVLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVELPNDAEENGNK 642
M V+ E + TD CPN RTKC D QTCC TKH SYHCCK+ANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEM KLIPALKV+DEPK NILEAKVNE DEVMCPNNKTKCKD KTCCITKHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFS+NNVELPNDAEENGNK
Sbjct: 97 MEMPVIEETDVKIITDQTCPNNRTKCQDSQTCCVTKHGSYHCCKFANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMRKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNKTKCKDIKTCCITKHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSLNNVELPNDAEENGNK 281 HSP 2 Score: 150.984 bits (380), Expect = 1.057e-44 Identity = 96/164 (58.54%), Postives = 117/164 (71.34%), Query Frame = 1
Query: 127 TTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNN--ILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVEL 612
T NICPNKRTKCT+RQTCCQTKHNSYHCCKYANAVCCPDKFHCCP G++CD+ KC + N+E K ++ EP I E V + CPNN+TKC+D++TCC+TKH +Y+CC NAVCC DK+ CCPEG+TCD K KC+K + S NVE+
Sbjct: 22 TVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPNGTSCDSVHKKCNR----NIEATK--SPIQTTPEPMEMPVIEETDVKIITDQTCPNNRTKCQDSQTCCVTKHGSYHCCKFANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKS--NVEM 177
BLAST of Granulin precursor vs. Planmine SMEST
Match: SMESG000018762.1 (SMESG000018762.1) HSP 1 Score: 280.026 bits (715), Expect = 1.263e-91 Identity = 160/204 (78.43%), Postives = 170/204 (83.33%), Query Frame = 1
Query: 31 ILGFNLLLIFVVKIYADGVMSFD-------------VLLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNN 603
IL N LL + + D +++ + LE KNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKV+DEPK NILEAKVNE DEVMCPNNKTKCKDTKTCCITKHNNYYCCPHKNAVCCSDKYQCCPEGT CD+KNKKCL +SF V +
Sbjct: 45 ILQCNSLLAPLYALAPDSLIALPDYRGVKTPDKLELISLEYLKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNKTKCKDTKTCCITKHNNYYCCPHKNAVCCSDKYQCCPEGTKCDLKNKKCLSASFVVES 248 HSP 2 Score: 276.559 bits (706), Expect = 2.410e-90 Identity = 153/164 (93.29%), Postives = 157/164 (95.73%), Query Frame = 1
Query: 112 NQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNN 603
N KNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKV+DEPK NILEAKVNE DEVMCPNNKTKCKDTKTCCITKHNNYYCCPHKNAVCCSDKYQCCPEGT CD+KNKKCL +SF V +
Sbjct: 307 NHKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNKTKCKDTKTCCITKHNNYYCCPHKNAVCCSDKYQCCPEGTKCDLKNKKCLSASFVVES 470 HSP 3 Score: 92.0485 bits (227), Expect = 2.327e-21 Identity = 65/170 (38.24%), Postives = 92/170 (54.12%), Query Frame = 1
Query: 103 LLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKC-----AKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSV 597
+LE + N + +CPN +TKC D +TCC TKHN+Y+CC + NAVCC DK+ CCPEG+ CD K KC+ +++NV+ +P E N L K+ + E C + K C K CC+ + CC + VCC D CCP T CD K +C+ + +
Sbjct: 396 ILEAKVNEKDEVMCPNNKTKCKDTKTCCITKHNNYYCCPHKNAVCCSDKYQCCPEGTKCDLKNKKCLSASFVVESQTNVDT---VPHQLFLAEQSNYELVFKLFKM-EKGCADQKHTCPGKKNCCVMDAGKFGCCKFNHGVCCPDLKTCCPHQTECDDKTNRCIANRLPL 561 HSP 4 Score: 61.6178 bits (148), Expect = 7.260e-11 Identity = 45/89 (50.56%), Postives = 60/89 (67.42%), Query Frame = 1
Query: 346 ALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVEL 612
+K D+ + LE N + +CPN +TKC D +TCC TKHN+Y+CC + NAVCC DK+ CCPEG+TCD K KC+K + S NVE+
Sbjct: 71 GVKTPDKLELISLEYLKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKS--NVEM 157 HSP 5 Score: 55.4546 bits (132), Expect = 7.221e-9 Identity = 52/128 (40.62%), Postives = 65/128 (50.78%), Query Frame = 1
Query: 238 CPDKFHCCPEGSTC---DAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVEL 612
C D+ H CP C DA + C K NV + CPN +TKC D +TCC TKHN+Y+CC + NAVCC DK+ CCPEG+TCD K KC+K + S NVE+
Sbjct: 280 CADQKHTCPGKKNCCVMDAGKFGCCKFNHKNVTTDNI--------------------------CPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKS--NVEM 379
BLAST of Granulin precursor vs. Planmine SMEST
Match: SMESG000018767.1 (SMESG000018767.1) HSP 1 Score: 275.789 bits (704), Expect = 1.026e-90 Identity = 153/164 (93.29%), Postives = 157/164 (95.73%), Query Frame = 1
Query: 112 NQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNN 603
N KNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKV+DEPK NILEAKVNE DEVMCPNNKTKCKDTKTCCITKHNNYYCCPHKNAVCCSDKYQCCPEGT CD+KNKKCL +SF V +
Sbjct: 241 NHKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNKTKCKDTKTCCITKHNNYYCCPHKNAVCCSDKYQCCPEGTKCDLKNKKCLSASFVVES 404 HSP 2 Score: 274.248 bits (700), Expect = 4.004e-90 Identity = 152/163 (93.25%), Postives = 157/163 (96.32%), Query Frame = 1
Query: 115 QKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNN 603
+KNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKV+DEPK NILEAKVNE DEVMCPNNKTKCKDTKTCCITKHNNYYCCPHKNAVCCSDKYQCCPEGT CD+KNKKCL +SF V +
Sbjct: 20 KKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVEDEPKTNILEAKVNEKDEVMCPNNKTKCKDTKTCCITKHNNYYCCPHKNAVCCSDKYQCCPEGTKCDLKNKKCLSASFVVES 182 HSP 3 Score: 111.694 bits (278), Expect = 2.216e-28 Identity = 86/208 (41.35%), Postives = 118/208 (56.73%), Query Frame = 1
Query: 103 LLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCA--------------------KSNVEM-GKLIPALKVDDEPKNN--------ILEAK---------VNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSVNNVEL 612
+LE + N + +CPN +TKC D +TCC TKHN+Y+CC + NAVCC DK+ CCPEG+ CD K KC+ + +SN E+ KL K + K+ +++A N + +CPN +TKC D +TCC TKHN+Y+CC + NAVCC DK+ CCPEG+TCD K KC+K + S NVE+
Sbjct: 108 ILEAKVNEKDEVMCPNNKTKCKDTKTCCITKHNNYYCCPHKNAVCCSDKYQCCPEGTKCDLKNKKCLSASFVVESQTNVDTVPHQLFLAEQSNYELVFKLFKMEKGCADQKHTCPGKKNCCVMDAGKFGCCKFNHKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKS--NVEM 313 HSP 4 Score: 91.6633 bits (226), Expect = 2.993e-21 Identity = 65/170 (38.24%), Postives = 92/170 (54.12%), Query Frame = 1
Query: 103 LLENQKNVTTDNICPNKRTKCTDRQTCCQTKHNSYHCCKYANAVCCPDKFHCCPEGSTCDAKQHKCVKC-----AKSNVEMGKLIPALKVDDEPKNNILEAKVNENDEVMCPNNXXXXXXXXXXXXXXHNNYYCCPHKNAVCCSDKYQCCPEGTTCDIKNKKCLKSSFSV 597
+LE + N + +CPN +TKC D +TCC TKHN+Y+CC + NAVCC DK+ CCPEG+ CD K KC+ +++NV+ +P E N L K+ + E C + K C K CC+ + CC + VCC D CCP T CD K +C+ + +
Sbjct: 330 ILEAKVNEKDEVMCPNNKTKCKDTKTCCITKHNNYYCCPHKNAVCCSDKYQCCPEGTKCDLKNKKCLSASFVVESQTNVDT---VPHQLFLAEQSNYELVFKLFKM-EKGCADQKHTCPGKKNCCVMDAGKFGCCKFNHGVCCPDLKTCCPHQTECDDKTNRCIANRLPL 495 The following BLAST results are available for this feature:
BLAST of Granulin precursor vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Granulin precursor vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 3
BLAST of Granulin precursor vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of Granulin precursor vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Granulin precursor vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 4
BLAST of Granulin precursor vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 4
BLAST of Granulin precursor vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Granulin precursor vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Granulin precursor vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Granulin precursor vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 2
BLAST of Granulin precursor vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Granulin precursor vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 1
BLAST of Granulin precursor vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Granulin precursor vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30019945 ID=SMED30019945|Name=Granulin precursor|organism=Schmidtea mediterranea sexual|type=transcript|length=673bpback to top protein sequence of SMED30019945-orf-1 >SMED30019945-orf-1 ID=SMED30019945-orf-1|Name=SMED30019945-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=211bp MKNFVAILGFNLLLIFVVKIYADGVMSFDVLLENQKNVTTDNICPNKRTKback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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