SMED30019904

Overview
NameSMED30019904
Smed IDSMED30019904
Length (bp)2478
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30019904 (SMED30019904) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30019904 (SMED30019904) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30019904

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30019904 dd_Smed_v4_11154_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30019904 dd_Smed_v4_11154_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30019904 dd_Smed_v4_11154_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30019904 dd_Smed_v4_11154_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30019904 dd_Smed_v4_11154_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30019904 dd_Smed_v4_11154_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30019904 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.003194:11054..13530 -12292
Homology
[Close]
BLAST of SMED30019904 vs. TrEMBL
Match: A0A2S2QJ61 (Uncharacterized protein OS=Sipha flava OX=143950 GN=g.157366 PE=4 SV=1)

HSP 1 Score: 111.309 bits (277), Expect = 3.595e-36
Identity = 63/179 (35.20%), Postives = 98/179 (54.75%), Query Frame = -1 
Query:  904 AVLKLPRGTGNFTADAVYKTMMRRGL*EKVSGMSFDSTSANFGFKNDACTNLERNVGRRLLSFGCRNYMYERVLQKIFSLQLGETFGPDIDIFKRFKL*WKFIDHGFF*TXXXXXXXXXXXXXXKEVL-QFSQLKLNDAQPHKDYLELQNLTIIFLGS*FPNSIHFKALGGLHNV*WMA 1437
             V K+  GTG   A AV++ +    L E+++ MSFD+T++N G    AC  LE+ + R LLS  CR+++ E +++++F+  +G + GPDI IF+RF   W  ID   F +   +  +  +   +K VL +F   +L   QP  DY E+  L++IFLG         +  G LH   WMA
Sbjct:   85 GVPKISNGTGEAQATAVFELLEDWKLTERINLMSFDTTASNSGVNTGACVLLEKKIKRDLLSLACRHHILELIIERVFNALMGASSGPDIQIFQRFSQNWNKIDTSKFESGIIEDTVANELNPQKNVLIKFINDQLATFQPRDDYKEMLQLSLIFLGDETAKDFTIRTPGALHRARWMA 263

HSP 2 Score: 46.9802 bits (110), Expect = 3.595e-36
Identity = 20/51 (39.22%), Postives = 34/51 (66.67%), Query Frame = -2 
Query: 1434 QELTVQIKTELVHKHTGPVIIHWEGKLLPDFTENCKVDRFPVLISGFGVQQ 1586
            ++++ +I++  +   T P+ IHW+GKLLP  T   KVDR  V++SG G+ +
Sbjct:   34 KKISDEIRSSFIT--TTPLTIHWDGKLLPALTSKEKVDRLAVIVSGDGIMK 82

HSP 3 Score: 45.4394 bits (106), Expect = 3.595e-36
Identity = 37/108 (34.26%), Postives = 57/108 (52.78%), Query Frame = -3 
Query:  599 LLYSFKVYMFGTTSNSKSHKAFKLTAKEE---DGLCFFCLPLLVA---TSKLGFLIPWLHPHQ*M-ISGF*NSTVNEVTAAAAIEKFRKNLWYLSEDLIVVFFFDLKI 901
            L+YS K+Y++ +         FKLTA+E         F + + +    TS  G L P  + +    +  + NS  N++ A AAI+ F ++LWYLSE LI + FFD K+
Sbjct:  265 LIYSMKIYLYRS--------QFKLTARELSVFQAFNIFVVKVYIKYWYTSSCGELAPNNNLNLLKELDHYKNS--NKLIADAAIKSFSRHLWYLSETLIGLAFFDYKV 362
[Close]
BLAST of SMED30019904 vs. TrEMBL
Match: A0A2A4JD51 (Uncharacterized protein (Fragment) OS=Heliothis virescens OX=7102 GN=B5V51_3559 PE=4 SV=1)

HSP 1 Score: 132.88 bits (333), Expect = 7.168e-35
Identity = 74/179 (41.34%), Postives = 101/179 (56.42%), Query Frame = -1 
Query:  904 AVLKLPRGTGNFTADAVYKTMMRRGL*EKVSGMSFDSTSANFGFKNDACTNLERNVGRRLLSFGCRNYMYERVLQKIFSLQLGETFGPDIDIFKRFKL*WKFIDHGFF*TXXXXXXXXXXXXXXKEV-LQFSQLKLNDAQPHKDYLELQNLTIIFLGS*FPNSIHFKALGGLHNV*WMA 1437
            AV KL RGTG   A AVY T++  GL +KV  M FD+T+ N G +N AC  LE+ + + +L   CR+++ E +L+   +  LG + GPDI IFKRFK  W  ID   + T  +D           +  + F+Q +LN+ QP  DY EL NLTII+LG      + F+   GLH   WMA
Sbjct:  295 AVPKLERGTGETCASAVYDTILEWGLNDKVKCMCFDTTAVNSGLRNGACVLLEQKMEKDMLWLACRHHILEIMLEAAINTALGPSSGPDILIFKRFKSLWNKIDQSDYKTVISDPSSLEYVQNIMQATISFAQNQLNEFQPRDDYKELLNLTIIYLGGVPEKGVLFRKPAGLHRARWMA 473

HSP 2 Score: 46.2098 bits (108), Expect = 7.168e-35
Identity = 30/69 (43.48%), Postives = 44/69 (63.77%), Query Frame = -2 
Query: 1434 LHLVINXXXXXXXXXXXRQELTVQIKTELVHKHTGPVIIHWEGKLLPDFTENCKVDRFPVLISGFGVQQ 1640
            +   +NRSSIR  R ++R+EL   +K E  +    P+ +HW+GKL+ D T +  VDR P+L+SG GV Q
Sbjct:  226 MEFSVNRSSIRRQRQKHRKELAESLKRE--YTTNLPLTVHWDGKLIEDITGHETVDRLPILVSGQGVDQ 292
[Close]
BLAST of SMED30019904 vs. TrEMBL
Match: J9LYI0 (Uncharacterized protein OS=Acyrthosiphon pisum OX=7029 PE=4 SV=2)

HSP 1 Score: 100.138 bits (248), Expect = 6.013e-33
Identity = 65/180 (36.11%), Postives = 92/180 (51.11%), Query Frame = -1 
Query:  904 AVLKLPRGTGNFTADAVYKTMMRRGL*EKVSGMSFDSTSANFGFKNDACTNLERNVGRRLLSFGCRNYMYERVLQKIFS-LQLGETFGPDIDIFKRFKL*WKFIDHGFF*T-XXXXXXXXXXXXXXKEVLQFSQLKLNDAQPHKDYLELQNLTIIFLGS*FPNSIHFKALGGLHNV*WMA 1437
             V  L  GTG   + AV+  ++   L +KV    FD+T++N G  N AC  LE+ + R +L F CR++++E VLQ +F+ +++    GPDI +FKRFK  WK ID   F   +           +   +  F   +LND  P  DY E   L +IFLG   P  I F+  G  H   WMA
Sbjct:  330 GVPALTSGTGEKISSAVHNLLLDWNLVDKVQAFVFDTTASNSGRLNGACVLLEQLLNRDILFFACRHHIFEIVLQAVFAHVKITVMNGPDIPLFKRFKNHWKNIDLSNFRIFSTFQHSHTNLKDVANNISLFCIQRLNDNFPRDDYREFLELVLIFLGGSPPRGIKFRQPGAYHLARWMA 509

HSP 2 Score: 47.7506 bits (112), Expect = 6.013e-33
Identity = 30/97 (30.93%), Postives = 55/97 (56.70%), Query Frame = -2 
Query: 1434 VLLMKVKLNNQKQVSQTLLQNELEKLSYLHLVINXXXXXXXXXXXRQELTVQIKTELVHKHTGPVIIHWEGKLLPDFTENCKVDRFPVLISGFGVQQ 1724
              L K K++++  V       E  +++ L   INRS+I+ +R  +R++ +  IK+  + ++   VI+HW+ KLLPD      +DR PV++S   V++
Sbjct:  231 AALDKCKISDRDAVHLLTAAAESFQVNCLEFTINRSTIKRARENFRKQSSEAIKSTFIDQNLNYVIVHWDSKLLPDILSKENIDRLPVIVSASNVEK 327

HSP 3 Score: 33.4982 bits (75), Expect = 6.013e-33
Identity = 14/28 (50.00%), Postives = 18/28 (64.29%), Query Frame = -2 
Query:  705 IVGGYIQAWISDSMAASTPMNDIRFLKQ 788
            IV  YIQ WI+     + PMND+ FLK+
Sbjct:  541 IVKCYIQEWITAPNPVTAPMNDLLFLKK 568

HSP 4 Score: 31.5722 bits (70), Expect = 6.013e-33
Identity = 18/50 (36.00%), Postives = 27/50 (54.00%), Query Frame = -3 
Query:  560 NSTVNEVTAAAAIEKFRKNLWYLSEDLIVVFFFDLKIPTNEKETMQRNIV 709
            N  + EV    AI KF  +LWYL+E+  V   FD ++    K+ M + I+
Sbjct:  573 NGKLGEV----AIRKFINHLWYLNEECAVFSIFDDRVNNETKQRMAKYIL 618
[Close]
BLAST of SMED30019904 vs. TrEMBL
Match: J9M593 (Uncharacterized protein OS=Acyrthosiphon pisum OX=7029 PE=4 SV=2)

HSP 1 Score: 106.301 bits (264), Expect = 3.023e-32
Identity = 64/178 (35.96%), Postives = 94/178 (52.81%), Query Frame = -1 
Query:  904 VLKLPRGTGNFTADAVYKTMMRRGL*EKVSGMSFDSTSANFGFKNDACTNLERNVGRRLLSFGCRNYMYERVLQKIFSLQLGETFGPDIDIFKRFKL*WKFID-HGFF*TXXXXXXXXXXXXXXKEVLQFSQLKLNDAQPHKDYLELQNLTIIFLGS*FPNSIHFKALGGLHNV*WMA 1434
            V +L  GTG   A AVY+ +   GL + V  +  D+T++N G    AC  LE+ + R +L   CR+++ E VL+  F +++     PD+ IFKRF+  W  I+ + F          +    ++ E+L F   +L   QP  DY EL  LTIIFLG   PN I FK  G +H+  WM+
Sbjct:  312 VPELISGTGENQATAVYQALENWGLTDNVQALCCDTTASNTGRLKGACILLEQKLEREILYLPCRHHILEVVLRSAFEIKIDIASAPDVPIFKRFQKCWPNINVNNFHIGLEDTFVFQSLQNLKDELLIFCINQLKQYQPRDDYKELLELTIIFLGQTPPNGIFFKVPGPIHHARWMS 489

HSP 2 Score: 49.6766 bits (117), Expect = 3.023e-32
Identity = 31/95 (32.63%), Postives = 53/95 (55.79%), Query Frame = -2 
Query: 1434 LMKVKLNNQKQVSQTLLQNELEKLSYLHLVINXXXXXXXXXXXRQELTVQIKTELVHKHTGPVIIHWEGKLLPDFTENCKVDRFPVLISGFGVQQ 1718
            L + K++++  V   L   E   ++   L+INR+S+   R R+R+     ++        GP ++HW+GKLLP  +E   VDR P++IS  G++Q
Sbjct:  214 LDRCKISDRDAVHILLATAESFGININELIINRTSVNSIRQRFRKNRIEALRVNFNPSQIGPCVVHWDGKLLPSLSEKKLVDRLPIIISYKGIEQ 308

HSP 3 Score: 34.6538 bits (78), Expect = 3.023e-32
Identity = 34/130 (26.15%), Postives = 61/130 (46.92%), Query Frame = -3 
Query:  542 LYSFKVYMFGTTSNSKSHKAFKLTAKEEDGL---CFFCLPLLVAT---SKLGFLIPWLHPHQ*MISGF*NSTVNEVTAAAAIEKFRKNLWYLSEDLIVVFFFDLKIPTNEK----ETMQ-RNIVVKRNDR 898
            +YS K+++F           FKLT KE + L   C F + + +     ++   L P +   Q +        ++E  + +A++K   +LWYL+ +   + FFD K+ +  K    + MQ R+I  + N R
Sbjct:  492 IYSIKIFLF--------RNEFKLTLKENNALRDLCIFIVRIYIKQWFCARAAALAPNMD-LQFIKDIILYEVIDEQISKSALKKMCGHLWYLTPEATSLAFFDDKVSSQTKIKMVKAMQSRDIESEANKR 612
[Close]
BLAST of SMED30019904 vs. TrEMBL
Match: J9M4P2 (Uncharacterized protein OS=Acyrthosiphon pisum OX=7029 PE=4 SV=2)

HSP 1 Score: 110.153 bits (274), Expect = 6.179e-32
Identity = 70/180 (38.89%), Postives = 95/180 (52.78%), Query Frame = -1 
Query:  904 AVLKLPRGTGNFTADAVYKTMMRRGL*EKVSGMSFDSTSANFGFKNDACTNLERNVGRRLLSFGCRNYMYERVLQKIFSL-QLGETFGPDIDIFKRFKL*WKFIDHGFF*T-XXXXXXXXXXXXXXKEVLQFSQLKLNDAQPHKDYLELQNLTIIFLGS*FPNSIHFKALGGLHNV*WMA 1437
             V  L  GTG   + AVY T+    + EKV    FD+T++N G  N +C  LE+ + R +L   CR++++E +LQ +FS  +L    GP+I IFKRFK  W  ID   + T  + +   KI   +  +V+ F +  LN   P  DY E   L IIFLG   P SIHFK  G  H   WM 
Sbjct:  427 GVPHLSSGTGKEISSAVYDTLKDWSMLEKVQAFVFDTTASNSGRLNGSCVLLEQMLNRPILFLACRHHIFEIILQSVFSYSKLTTMSGPEIPIFKRFKNNWNQIDQTKYNTWVSDNEVKKILHKVADDVIIFCKDTLNQNLPRDDYKEFLELVIIFLGGVPPKSIHFKRPGAYHLARWMC 606

HSP 2 Score: 39.6614 bits (91), Expect = 6.179e-32
Identity = 29/95 (30.53%), Postives = 53/95 (55.79%), Query Frame = -2 
Query: 1434 LMKVKLNNQKQVSQTLLQNELEKLSYLHLVINXXXXXXXXXXXRQELTVQIKTELVHKHTGPVIIHWEGKLLPDFTENCKVDRFPVLISGFGVQQ 1718
            L K K++++  V   +   E+  ++     INRSS++ SR  +R +++ +IKT     +    ++HW+ K+LP+      VDR PV+I+   V+Q
Sbjct:  330 LDKCKVSDRDAVHLLIACAEVFNVNVNDYAINRSSVKRSREGFRYQISSEIKTAFHQLNLNFAVVHWDSKILPNLIGTENVDRLPVIITAPNVEQ 424

HSP 3 Score: 31.5722 bits (70), Expect = 6.179e-32
Identity = 15/42 (35.71%), Postives = 24/42 (57.14%), Query Frame = -3 
Query:  560 AAAAIEKFRKNLWYLSEDLIVVFFFDLKIPTNEKETMQRNIV 685
            A  AI+KF  +LWYL E+      FD +I  + K+ M + ++
Sbjct:  677 AELAIKKFINHLWYLGEETACFSLFDDRIENHVKKQMAQQLL 718

HSP 4 Score: 28.1054 bits (61), Expect = 6.179e-32
Identity = 11/28 (39.29%), Postives = 15/28 (53.57%), Query Frame = -2 
Query:  705 IVGGYIQAWISDSMAASTPMNDIRFLKQ 788
            IV  YI+ W     A   P ND+ FL++
Sbjct:  638 IVKCYIEFWFRSPNAIEAPYNDVLFLRK 665
[Close]
BLAST of SMED30019904 vs. Planmine SMEST
Match: SMESG000078572.1 (SMESG000078572.1)

HSP 1 Score: 86.2705 bits (212), Expect = 1.579e-25
Identity = 48/67 (71.64%), Postives = 56/67 (83.58%), Query Frame = -2 
Query: 1434 LVINXXXXXXXXXXXRQELTVQIKTELVHKHTGPVIIHWEGKLLPDFTENCKVDRFPVLISGFGVQQ 1634
            LVINRSSIR +RMR +QE+ +QIKTE  HKH+ PVIIHW+ +LLPDFTENCKVD   VL+S FGVQQ
Sbjct:   12 LVINRSSIRRTRMRSQQEVAMQIKTEFTHKHSRPVIIHWDSELLPDFTENCKVDHLHVLVSRFGVQQ 78

HSP 2 Score: 50.0618 bits (118), Expect = 1.579e-25
Identity = 26/40 (65.00%), Postives = 29/40 (72.50%), Query Frame = -1 
Query: 1318 AVLKLPRGTGNFTADAVYKTMMRRGL*EKVSGMSFDSTSA 1437
            AV KL R T NFTADAVY+T+   GL EKV G +FD TSA
Sbjct:   81 AVPKLTRRTNNFTADAVYETVKEWGLQEKVCGTAFDMTSA 120

HSP 3 Score: 76.6406 bits (187), Expect = 2.465e-18
Identity = 40/59 (67.80%), Postives = 46/59 (77.97%), Query Frame = -3 
Query:  533 STVNEVTAAAAIEKFRKNLWYLSEDLIVVFFFDLKIPTNEKETMQRNI-VVKRNDRQNK 706
            STV+EV AAA IE FRK+LWYLSEDLI + FFDLKIP+  KE+M RNI +VK ND   K
Sbjct:  139 STVSEVIAAAPIETFRKHLWYLSEDLIGLSFFDLKIPSYGKESMLRNIPLVKGNDNPPK 197

HSP 4 Score: 31.187 bits (69), Expect = 2.465e-18
Identity = 17/32 (53.12%), Postives = 18/32 (56.25%), Query Frame = -2 
Query:  471 AKQKLFF--------FPDTAPKTWVENLDYMQ 542
            +K K FF        F DTAPKTWVEN D  Q
Sbjct:  217 SKIKAFFSAPNINVNFLDTAPKTWVENPDSQQ 248

HSP 5 Score: 24.2534 bits (51), Expect = 2.465e-18
Identity = 13/27 (48.15%), Postives = 16/27 (59.26%), Query Frame = -2 
Query:  690 QAWISDSMAASTPMNDIRFLKQYCQRS 770
             AWIS S++      D+RFLKQ  Q S
Sbjct:  119 SAWISASIS------DVRFLKQLQQYS 139
[Close]
BLAST of SMED30019904 vs. Planmine SMEST
Match: SMESG000028508.1 (SMESG000028508.1)

HSP 1 Score: 82.0333 bits (201), Expect = 2.192e-20
Identity = 47/148 (31.76%), Postives = 75/148 (50.68%), Query Frame = -1 
Query:  904 GMSFDSTSANFGFKNDACTNLERNVGRRLLSFGCRNYMYERVLQKIFSLQLGETFGPDIDIFKRFKL*WKFIDHGFF*T-XXXXXXXXXXXXXXKEVLQFSQLKLNDAQPHKDYLELQNLTIIFLGS*FPNSIHFKALGGLHNV*WMA 1344
            G      + N    N AC  LE+ +G  LLS  CR++++E V+  +F   +G +   ++ +FKRF+  W+FID   + T         +    R ++L+F++ +L   QP  DY E   L+IIFLG      I F A G + +  WM+
Sbjct:  747 GQDMKDLALNRSLANGACVLLEQMLGINLLSLACRHHIFELVIGAVFQTCMGPSSAQEVLMFKRFQSCWQFIDPASYSTGMDDKDVAAVLMDTRNKLLEFAESQLQIMQPRDDYREFLELSIIFLGGVPARGIRFIAPGAMQHARWMS 894

HSP 2 Score: 37.3502 bits (85), Expect = 2.192e-20
Identity = 37/120 (30.83%), Postives = 51/120 (42.50%), Query Frame = -3 
Query:  575 LLYSFKVYMFGTTSNSKSHKAFKLTAKEEDGL---CFFCLPLLVATSKLGFLIP--WLHPH------Q*MISGF*NSTVNEVTAAAAIEKFRKNLWYLSEDLIVVFFFDLKIPTNEKETM 901
            +LYS KVYMF           F L   E  GL   C F + L +   K+ F  P     P       Q +I       V+   + AA  K  K+LWY+SE+L+ +  FD  + T  K  +
Sbjct:  896 VLYSLKVYMF--------RSQFDLRPTEVKGLRDICTFSVSLYI---KVWFTAPCAATGPRNDLTFLQALID---YEVVHPAISKAASAKMAKHLWYISEELVGLALFDKDLSTTTKREL 1001
[Close]
BLAST of SMED30019904 vs. Planmine SMEST
Match: SMESG000074894.1 (SMESG000074894.1)

HSP 1 Score: 77.7962 bits (190), Expect = 2.091e-19
Identity = 39/59 (66.10%), Postives = 46/59 (77.97%), Query Frame = -3 
Query:  533 STVNEVTAAAAIEKFRKNLWYLSEDLIVVFFFDLKIPTNEKETMQRNI-VVKRNDRQNK 706
            S+VN+V AA A+EKFRK+LWYL+ED I + FFDLKIPT E E MQRNI +VK ND   K
Sbjct:   27 SSVNKVIAAEAVEKFRKHLWYLNEDFIGLSFFDLKIPTYETEAMQRNILLVKGNDNPPK 85

HSP 2 Score: 38.1206 bits (87), Expect = 2.091e-19
Identity = 18/26 (69.23%), Postives = 18/26 (69.23%), Query Frame = -2 
Query:  690 AWISDSMAASTPMNDIRFLKQYCQRS 767
            AWIS  M AS PMNDIR LKQ  Q S
Sbjct:    2 AWISPFMTASAPMNDIRLLKQLQQYS 27
[Close]
BLAST of SMED30019904 vs. Planmine SMEST
Match: SMESG000033311.1 (SMESG000033311.1)

HSP 1 Score: 68.5514 bits (166), Expect = 2.366e-14
Identity = 37/105 (35.24%), Postives = 57/105 (54.29%), Query Frame = -1 
Query: 1126 AVLKLPRGTGNFTADAVYKTMMR-RGL*EKVSGMSFDSTSANFGFKNDACTNLERNVGRRLLSFGCRNYMYERVLQKIFSLQLGETFGPDIDIFKRFKL*WKFID 1437
             + KLP  TG    + V  T+   + + + ++G  FD+TS+N      A T ++R   +RLL   CR+++ E ++    S Q  E+ GP I +F RFK  WKFID
Sbjct:   87 VIPKLPVSTGELMGNTVVHTLQTWKCVSDWLAGQCFDTTSSNTSINTGAITVIQRAFDKRLLFLACRHHILE-IVSAAVSYQFFESSGPQIALFGRFKQQWKFID 190

HSP 2 Score: 30.0314 bits (66), Expect = 2.366e-14
Identity = 13/38 (34.21%), Postives = 23/38 (60.53%), Query Frame = -2 
Query: 1434 TGPVIIHWEGKLLPDFTENCK--VDRFPVLISGFGVQQ 1541
            T P+I H++GK LPD     +  VDR  +++S   +++
Sbjct:   47 TTPLIAHFDGKSLPDNDGRTEELVDRMTIVVSALNIEK 84
[Close]
BLAST of SMED30019904 vs. Planmine SMEST
Match: SMESG000014243.1 (SMESG000014243.1)

HSP 1 Score: 53.9138 bits (128), Expect = 3.066e-12
Identity = 31/98 (31.63%), Postives = 53/98 (54.08%), Query Frame = -1 
Query: 1147 AVLKLPRGTGNFTADAVYKTMMR-RGL*EKVSGMSFDSTSANFGFKNDACTNLERNVGRRLLSFGCRNYMYERVLQKIFSLQLGETFGPDIDIFKRFK 1437
            A+ KLP  TG    +AV  T+   +G+ + ++G+ FD+T +N G    A   ++R   +RLL   CR+++ E V   +F  +  ++ GP I +F   K
Sbjct:   87 AIPKLPVSTGELMGNAVVYTLQAWKGVPDWLAGLCFDTTISNTGINIGAINVIQRAFDKRLLFLACRHHILEIVSAAVFD-KFFKSSGPQIALFAASK 183

HSP 2 Score: 37.7354 bits (86), Expect = 3.066e-12
Identity = 16/38 (42.11%), Postives = 26/38 (68.42%), Query Frame = -2 
Query: 1434 TGPVIIHWEGKLLPDFTENCK--VDRFPVLISGFGVQQ 1541
            T P+I H++GKLLPD     +  VDR P+++SG  +++
Sbjct:   47 TTPLITHFDGKLLPDNDGRTEELVDRMPIVVSGLSIEK 84
The following BLAST results are available for this feature:
BLAST of SMED30019904 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019904 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019904 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019904 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019904 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019904 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019904 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019904 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A2S2QJ613.595e-3635.20Uncharacterized protein OS=Sipha flava OX=143950 G... [more]
A0A2A4JD517.168e-3541.34Uncharacterized protein (Fragment) OS=Heliothis vi... [more]
J9LYI06.013e-3336.11Uncharacterized protein OS=Acyrthosiphon pisum OX=... [more]
J9M5933.023e-3235.96Uncharacterized protein OS=Acyrthosiphon pisum OX=... [more]
J9M4P26.179e-3238.89Uncharacterized protein OS=Acyrthosiphon pisum OX=... [more]
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BLAST of SMED30019904 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019904 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019904 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019904 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019904 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019904 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000078572.11.579e-2571.64SMESG000078572.1[more]
SMESG000028508.12.192e-2031.76SMESG000028508.1[more]
SMESG000074894.12.091e-1966.10SMESG000074894.1[more]
SMESG000033311.12.366e-1435.24SMESG000033311.1[more]
SMESG000014243.13.066e-1231.63SMESG000014243.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30019904 ID=SMED30019904|Name=SMED30019904|organism=Schmidtea mediterranea sexual|type=transcript|length=2478bp
CTCAATTGCAGTGTCTGAAATAGTGTGCAAATGCGCACAATATATGCAGT
AGGATGGTCCTTATTTTCAAAAGTTGAAAGTTTAATGGCCCCACCCTTTA
AAAAGGTTCCATTTGATGAAAAAGCATACGTGGTGAAATATTTTTGAAAT
CGGATTACATTGTAATCCTGAGCGTTTGCCATATATTTCGGTTAATTACG
TTTGTTCCCGTAATAAGCTAAGTTTTCACAAAACAACTGCATATTGTGTA
TAATTATGAAAAACATGGAATAATAAGTACTAATGCGACGTCCAACAATA
ATGAGTAAAACATCAAACACAAGTTATCTTGCGAAGATCGGGAAATCAAT
TTCGGTGTTGGGAGATGACTTGGAGAAGATATTGCTTCTGCTCTTCATTG
TGAGTAATTTGTGCATTTAATTGTTCTATTAAGGCAACACCTATTTCCGC
AAAATAATATTTTGAAGTTGTTGCATGTAATCGAGGTTTTCTACCCATGT
TTTAGGAGCAGTGTCAGGGAAGAAGAAAAGCTTTTGTTTTGCCGGTCATT
TCGTTTAACGACGATATTTCTTTGCATTGTTTCTTTTTCATTTGTCGGTA
TTTTGAGGTCAAAGAAAAATACGACAATAAGGTCTTCACTCAGATACCAT
AAGTTTTTGCGAAATTTTTCAATGGCTGCTGCAGCAGTAACTTCGTTGAC
AGTACTGTTTCAAAAACCTGATATCATTCATTGGTGTGGATGCAGCCATG
GAATCAGAAATCCAAGCTTGGATGTAGCCACCAACAATGGCAAACAAAAG
AAACATAATCCATCTTCTTCTTTTGCTGTGAGTTTGAAAGCCTTATGACT
TTTACTATTCGATGTTGTGCCAAACATGTAAACTTTGAAAGAATATAGAA
GAAGGCCATCCATCAAACATTGTGCAGACCACCAAGAGCTTTGAAATGAA
TGCTATTAGGAAATTAGCTTCCAAGAAATATTATTGTTAAATTCTGTAAT
TCAAGGTAGTCCTTGTGTGGCTGAGCGTCATTGAGTTTCAGCTGACTAAA
TTGAAGCACTTCTTTTCGAATAATGTCATCAATCTTCATCTTTATATCTG
CTGCAGCTGTTCAAAAAAAACCATGATCAATAAACTTCCATTACAACTTA
AAACGCTTGAAAATGTCAATATCAGGTCCGAAAGTCTCTCCTAATTGCAA
GCTGAATATTTTCTGAAGCACTCTTTCATACATGTAATTTCGGCATCCAA
ACGAAAGCAATCGTCTACCGACGTTTCTTTCGAGATTAGTACAGGCATCA
TTTTTGAAACCAAAGTTCGCTGAAGTTGAATCAAATGACATTCCAGATAC
CTTCTCCTAGAGACCCCGTCTCATCATAGTTTTGTAAACAGCATCGGCGG
TAAAATTTCCAGTTCCTCTTGGTAGCTTTAGCACTGCTGCACTCCAAATC
CCGATATCAACACAGGAAAACGATCCACTTTACAGTTTTCAGTGAAATCT
GGCAGGAGTTTACCCTCCCAGTGTATGATGACGGGTCCAGTGTGCTTGTG
TACTAACTCAGTCTTGATCTGCACTGTCAGTTCTTGACGATACCGCATTC
TTGATCAACGAATTGACGACCTATTGATGACTAGATGAAGATACGAAAGT
TTTTCTAGCTCGTTTTGAAGGAGGGTTTGAGAAACCTGTTTCTGGTTGTT
CAACTTCACTTTCATCAATAAAACTAGAGTCCCGTTCTATTGTTTCATTT
GGGCTTGTTGAGAATTCGCAAGTTCATATTTTAAGATCAAAGTGTTTTTC
TCTTGTGGATTCTCTATCTTTTCCCATTAATACTATTTATACAACCCTGG
CGACCTCCACGCTGATCAATAACAAATTCATTATCTTCCTNGGATTGTCA
TCAAGTCTAGTGCATCTTGATGAGCGATTTCAAATAATTTTGAAAACAGC
TGAACGAATTGGTTCACTTTCTTGAATTGAGGATCCAACTTAGGTAATCT
GTTCCCCTGAAGATTTTTCAAGACACTGAACAATGTTTTGATTTTTTAAA
TCACATGACTTTTAGCTTTAATAGGAATAACTACTTTTCCCAGAAGAGTA
TAACAAGCTCTGCTGTTTGCGTAGAACTTTCCCTGATTGTAAGCTTGGAA
TCATTATGAAATGAAAAAAAAGCTGAAGTGCTTTGAGATTAGATGGCAAC
TTGGATCCTTTTATGCCATCGCATATGCTTCCAATAAGATAAATCTCCTT
GCTTTTACGTGTACAAGCCATCAAATTGTCAACAACATTTAAAACAGAAA
ATTAATGAAAACAAATTAAAATCAAGATTAGAGCTTCTGCAATGTTTCTA
TTTATCTTTGTGTTAATTCTTACAATGAAATAAAAGAACATTCCTCACTT
TTATATTTGTTAGACATATTAAAATGTATTTAACATAACTATCATTATAA
CTATTTACCTGAGACTGAGTTTAATTTT
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron