SMED30019845

Overview
NameSMED30019845
Smed IDSMED30019845
Length (bp)748
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30019845 (SMED30019845) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30019845 (SMED30019845) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30019845 (SMED30019845) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30019845 (SMED30019845) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30019845

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30019845SMESG000032451.1 SMESG000032442.1 SmedASXL_015319SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30019845 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.001637:70334..71401 -3546
v31.009444:15450..16518 +3535
Homology
[Close]
BLAST of SMED30019845 vs. Ensembl Human
Match: GEMIN2 (gem nuclear organelle associated protein 2 [Source:HGNC Symbol;Acc:HGNC:10884])

HSP 1 Score: 63.929 bits (154), Expect = 1.902e-11
Identity = 43/144 (29.86%), Postives = 70/144 (48.61%), Query Frame = 2 
Query:  359 PDIDDKEVWLGWVLGKNFNSGDNKGH--------------LEPLTSIICGMSQKQIIRSIEYLAD-LDDLPYTHLIGRWIYALLSVLSVPLNHEAAYLIRSLARHL--IGHMKNSNDSNSIYKSMNAVLSIIIYSFKQIDLINE 739
            P  +D+E W  + LG+   +    G                 PL SI+  M+Q  +   +EYL++   +  +T  +GRW+YALL+ L  PL  EA  LIR LAR    +  + +S D   +  ++N ++ ++   F Q DL +E
Sbjct:  136 PKSEDEEGWKKFCLGEKLCADGAVGPATNESPGIDYVQIGFPPLLSIVSRMNQATVTSVLEYLSNWFGERDFTPELGRWLYALLACLEKPLLPEAHSLIRQLARRCSEVRLLVDSKDDERV-PALNLLICLVSRYFDQRDLADE 278
[Close]
BLAST of SMED30019845 vs. Ensembl Human
Match: GEMIN2 (gem nuclear organelle associated protein 2 [Source:HGNC Symbol;Acc:HGNC:10884])

HSP 1 Score: 60.077 bits (144), Expect = 3.653e-10
Identity = 41/132 (31.06%), Postives = 69/132 (52.27%), Query Frame = 2 
Query:  359 PDIDDKEVWLGWVLGKNFNSGDNKGHLEPLTSIICGMS--QKQIIRSIEYLAD-LDDLPYTHLIGRWIYALLSVLSVPLNHEAAYLIRSLARHL--IGHMKNSNDSNSIYKSMNAVLSIIIYSFKQIDLINE 739
            P  +D+E W  + LG+   +    G + P T+   G+   Q  +   +EYL++   +  +T  +GRW+YALL+ L  PL  EA  LIR LAR    +  + +S D   +  ++N ++ ++   F Q DL +E
Sbjct:  136 PKSEDEEGWKKFCLGEKLCA---DGAVGPATNESPGIDYVQATVTSVLEYLSNWFGERDFTPELGRWLYALLACLEKPLLPEAHSLIRQLARRCSEVRLLVDSKDDERV-PALNLLICLVSRYFDQRDLADE 263
[Close]
BLAST of SMED30019845 vs. Ensembl Human
Match: GEMIN2 (gem nuclear organelle associated protein 2 [Source:HGNC Symbol;Acc:HGNC:10884])

HSP 1 Score: 56.6102 bits (135), Expect = 5.032e-9
Identity = 35/105 (33.33%), Postives = 51/105 (48.57%), Query Frame = 2 
Query:  359 PDIDDKEVWLGWVLGKNFNSGDNKGH--------------LEPLTSIICGMSQKQIIRSIEYLAD-LDDLPYTHLIGRWIYALLSVLSVPLNHEAAYLIRSLARH 628
            P  +D+E W  + LG+   +    G                 PL SI+  M+Q  +   +EYL++   +  +T  +GRW+YALL+ L  PL  EA  LIR LAR 
Sbjct:  136 PKSEDEEGWKKFCLGEKLCADGAVGPATNESPGIDYVQIGFPPLLSIVSRMNQATVTSVLEYLSNWFGERDFTPELGRWLYALLACLEKPLLPEAHSLIRQLARR 240
[Close]
BLAST of SMED30019845 vs. Ensembl Human
Match: GEMIN2 (gem nuclear organelle associated protein 2 [Source:HGNC Symbol;Acc:HGNC:10884])

HSP 1 Score: 56.6102 bits (135), Expect = 6.115e-9
Identity = 35/105 (33.33%), Postives = 51/105 (48.57%), Query Frame = 2 
Query:  359 PDIDDKEVWLGWVLGKNFNSGDNKGH--------------LEPLTSIICGMSQKQIIRSIEYLAD-LDDLPYTHLIGRWIYALLSVLSVPLNHEAAYLIRSLARH 628
            P  +D+E W  + LG+   +    G                 PL SI+  M+Q  +   +EYL++   +  +T  +GRW+YALL+ L  PL  EA  LIR LAR 
Sbjct:  125 PKSEDEEGWKKFCLGEKLCADGAVGPATNESPGIDYVQIGFPPLLSIVSRMNQATVTSVLEYLSNWFGERDFTPELGRWLYALLACLEKPLLPEAHSLIRQLARR 229
[Close]
BLAST of SMED30019845 vs. Ensembl Zebrafish
Match: gemin2 (gem (nuclear organelle) associated protein 2 [Source:ZFIN;Acc:ZDB-GENE-030131-3756])

HSP 1 Score: 63.1586 bits (152), Expect = 2.299e-11
Identity = 62/227 (27.31%), Postives = 98/227 (43.17%), Query Frame = 2 
Query:  122 FYQNESKKLPCVTKRTVQNITPTKLGISYIILKNEAVLK-PENEYHLSDVNFQIVNEFEHLRNILHTKTSN-KSSLVIYN--YPDIDDKEVWLGWVLGKNF--------NSGDNKG------HLEPLTSIICGMSQKQIIRSIEYLAD-LDDLPYTHLIGRWIYALLSVLSVPLNHEAAYLIRSLARHLIGHMKN--SNDSNSIYKSMNAVLSIIIYSFKQIDLINE 739
            F Q E+   P V    V  I P KL    + +     L  PE          Q V+ F  +R  ++    + K+  +  N   P  +D++ W  +  G+N           G N G         P  SI+  +SQ  +   +EYL +  ++  +   +GRW+YALL+ L  PL  EA  LIR LAR       N  S D + +  ++N ++ I+   F+Q DL ++
Sbjct:   38 FVQREASLCPDVV---VAQIDPKKLKKQSVNVSITGCLAAPEGFSPSFKWQQQQVSRFSEVRQSVNKHRQHWKAQFLDDNVVMPKTEDEDGWKRFCFGENVCLEIQLKEEDGVNPGLDYVKTGFPPFLSIVSKLSQSTVSAVLEYLINWFEERDFVPQLGRWLYALLACLEKPLLPEAHSLIRQLARRCSAVRANLESKDDDRL-SALNLMICIVARYFEQNDLADK 260
[Close]
BLAST of SMED30019845 vs. Ensembl Zebrafish
Match: gemin2 (gem (nuclear organelle) associated protein 2 [Source:ZFIN;Acc:ZDB-GENE-030131-3756])

HSP 1 Score: 62.7734 bits (151), Expect = 2.371e-11
Identity = 62/227 (27.31%), Postives = 98/227 (43.17%), Query Frame = 2 
Query:  122 FYQNESKKLPCVTKRTVQNITPTKLGISYIILKNEAVLK-PENEYHLSDVNFQIVNEFEHLRNILHTKTSN-KSSLVIYN--YPDIDDKEVWLGWVLGKNF--------NSGDNKG------HLEPLTSIICGMSQKQIIRSIEYLAD-LDDLPYTHLIGRWIYALLSVLSVPLNHEAAYLIRSLARHLIGHMKN--SNDSNSIYKSMNAVLSIIIYSFKQIDLINE 739
            F Q E+   P V    V  I P KL    + +     L  PE          Q V+ F  +R  ++    + K+  +  N   P  +D++ W  +  G+N           G N G         P  SI+  +SQ  +   +EYL +  ++  +   +GRW+YALL+ L  PL  EA  LIR LAR       N  S D + +  ++N ++ I+   F+Q DL ++
Sbjct:   30 FVQREASLCPDVV---VAQIDPKKLKKQSVNVSITGCLAAPEGFSPSFKWQQQQVSRFSEVRQSVNKHRQHWKAQFLDDNVVMPKTEDEDGWKRFCFGENVCLEIQLKEEDGVNPGLDYVKTGFPPFLSIVSKLSQSTVSAVLEYLINWFEERDFVPQLGRWLYALLACLEKPLLPEAHSLIRQLARRCSAVRANLESKDDDRL-SALNLMICIVARYFEQNDLADK 252
[Close]
BLAST of SMED30019845 vs. Ensembl Xenopus
Match: ndufaf1 (NADH:ubiquinone oxidoreductase complex assembly factor 1 [Source:Xenbase;Acc:XB-GENE-1007618])

HSP 1 Score: 68.1662 bits (165), Expect = 5.422e-13
Identity = 64/240 (26.67%), Postives = 106/240 (44.17%), Query Frame = 2 
Query:   89 KIHSSYLQMIIFYQNESKKLPCVTKRTVQNITPTKLGISYIILKNEAVLKPENEYHLSDVNFQI--VNEFEHLRNILHTKTSNKSSLVI---YNYPDIDDKEVWLGWVLGKNF--------NSGDN-------KGHLEPLTSIICGMSQKQIIRSIEYLAD-LDDLPYTHLIGRWIYALLSVLSVPLNHEAAYLIRSLAR---HLIGHMKNSNDSNSIYKSMNAVLSIIIYSFKQIDLIN 736
            +    YL+ +   Q E+ + P V    +  I P KL     +  + +  +P  E +   + +Q   V +F  +R  LH   S+  S  +      P  +D+E W  + LG+          NS          K    PL SI+  MSQ  +   +EYL +  ++  +T  +GRW+YALL+ L  PL  EA  LIR LAR    +   +++  D      ++N  + ++   F+Q DL +
Sbjct:   31 RTPQEYLRRV---QIEAARCPDVV---IAQIDPKKLRKKQTVSISLSGCQPAPEGYSPSLRWQQQQVAQFSAVRQSLHKHRSHWRSQPLDSNVTMPSTEDEESWKKFCLGERLYSDLAAALNSDSQHPGIDYIKVGFPPLLSIVSRMSQATVTSVLEYLVNWFEERNFTPELGRWLYALLACLEKPLLPEAHSLIRQLARRCSQVRAGVEHKEDER--VSALNLFICLVGRYFEQRDLAD 262
[Close]
BLAST of SMED30019845 vs. Ensembl Xenopus
Match: ndufaf1 (NADH:ubiquinone oxidoreductase complex assembly factor 1 [Source:Xenbase;Acc:XB-GENE-1007618])

HSP 1 Score: 65.4698 bits (158), Expect = 3.497e-12
Identity = 58/201 (28.86%), Postives = 90/201 (44.78%), Query Frame = 2 
Query:   89 KIHSSYLQMIIFYQNESKKLPCVTKRTVQNITPTKLGISYIILKNEAVLKPENEYHLSDVNFQI--VNEFEHLRNILHTKTSNKSSLVI---YNYPDIDDKEVWLGWVLGKNFNS-------GDN--------KGHLEPLTSIICGMSQKQIIRSIEYLAD-LDDLPYTHLIGRWIYALLSVLSVPLNHEAAYLIRSLARH 628
            +    YL+ +   Q E+ + P V    +  I P KL     +  + +  +P  E +   + +Q   V +F  +R  LH   S+  S  +      P  +D+E W  + LG+   S        D+        K    PL SI+  MSQ  +   +EYL +  ++  +T  +GRW+YALL+ L  PL  EA  LIR LAR 
Sbjct:   31 RTPQEYLRRV---QIEAARCPDVV---IAQIDPKKLRKKQTVSISLSGCQPAPEGYSPSLRWQQQQVAQFSAVRQSLHKHRSHWRSQPLDSNVTMPSTEDEESWKKFCLGERLYSDLAAALNSDSQHPGIDYIKVGFPPLLSIVSRMSQATVTSVLEYLVNWFEERNFTPELGRWLYALLACLEKPLLPEAHSLIRQLARR 225
[Close]
BLAST of SMED30019845 vs. Ensembl Mouse
Match: Gemin2 (gem nuclear organelle associated protein 2 [Source:MGI Symbol;Acc:MGI:1913853])

HSP 1 Score: 66.2402 bits (160), Expect = 1.930e-12
Identity = 65/239 (27.20%), Postives = 106/239 (44.35%), Query Frame = 2 
Query:   89 KIHSSYLQMIIFYQNESKKLPCVTKRTVQNITPTKLGISYIILKNEAVLKPENEYHLSDVNFQI--VNEFEHLRNILHTKTSN-KSSLVIYN--YPDIDDKEVWLGWVLGKNFNSGDNKGH--------------LEPLTSIICGMSQKQIIRSIEYLAD-LDDLPYTHLIGRWIYALLSVLSVPLNHEAAYLIRSLARHL--IGHMKNSNDSNSIYKSMNAVLSIIIYSFKQIDLINE 739
            +    YL+ +   Q E+ + P V    V  I P KL     +  + +  +P  E +   + +Q   V  F  +R  +H   ++ KS  +  N   P  +D+E W  + LG+   +    G                 PL SI+  M+Q  I   +EYL++   +  +T  +GRW YALL+ L  PL  EA  LIR LAR    +  +  S D   +  ++N ++ ++   F Q DL +E
Sbjct:   36 RTPQEYLRRV---QIEAAQCPDVV---VAQIDPKKLKRKQSVNISLSGCQPAPEGYSPTLQWQQQQVAHFSTVRQSVHKHRNHWKSQQLDSNVAMPKSEDEEGWKKFCLGERLCAEGATGPSTEESPGIDYVQVGFPPLLSIVSRMNQTTITSVLEYLSNWFGERDFTPELGRWFYALLACLEKPLLPEAHSLIRQLARRCSEVRLLVGSKDDERV-PALNLLICLVSRYFDQRDLADE 267
[Close]
BLAST of SMED30019845 vs. UniProt/SwissProt
Match: sp|Q9CQQ4|GEMI2_MOUSE (Gem-associated protein 2 OS=Mus musculus OX=10090 GN=Gemin2 PE=2 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 1.350e-11
Identity = 65/239 (27.20%), Postives = 106/239 (44.35%), Query Frame = 2 
Query:   89 KIHSSYLQMIIFYQNESKKLPCVTKRTVQNITPTKLGISYIILKNEAVLKPENEYHLSDVNFQI--VNEFEHLRNILHTKTSN-KSSLVIYN--YPDIDDKEVWLGWVLGKNFNSGDNKGH--------------LEPLTSIICGMSQKQIIRSIEYLAD-LDDLPYTHLIGRWIYALLSVLSVPLNHEAAYLIRSLARHL--IGHMKNSNDSNSIYKSMNAVLSIIIYSFKQIDLINE 739
            +    YL+ +   Q E+ + P V    V  I P KL     +  + +  +P  E +   + +Q   V  F  +R  +H   ++ KS  +  N   P  +D+E W  + LG+   +    G                 PL SI+  M+Q  I   +EYL++   +  +T  +GRW YALL+ L  PL  EA  LIR LAR    +  +  S D   +  ++N ++ ++   F Q DL +E
Sbjct:   36 RTPQEYLRRV---QIEAAQCPDVV---VAQIDPKKLKRKQSVNISLSGCQPAPEGYSPTLQWQQQQVAHFSTVRQSVHKHRNHWKSQQLDSNVAMPKSEDEEGWKKFCLGERLCAEGATGPSTEESPGIDYVQVGFPPLLSIVSRMNQTTITSVLEYLSNWFGERDFTPELGRWFYALLACLEKPLLPEAHSLIRQLARRCSEVRLLVGSKDDERV-PALNLLICLVSRYFDQRDLADE 267
[Close]
BLAST of SMED30019845 vs. UniProt/SwissProt
Match: sp|O42260|GEMI2_XENLA (Gem-associated protein 2 OS=Xenopus laevis OX=8355 GN=gemin2 PE=2 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 4.666e-11
Identity = 64/241 (26.56%), Postives = 103/241 (42.74%), Query Frame = 2 
Query:   89 KIHSSYLQMIIFYQNESKKLPCVTKRTVQNITPTKLGISYIILKNEAVLKPENEYHLSDVNFQI--VNEFEHLRNILHTKTSNKSSLVI---YNYPDIDDKEVWLGWVLGKNF--------NSGDN-------KGHLEPLTSIICGMSQKQIIRSIEYLAD-LDDLPYTHLIGRWIYALLSVLSVPLNHEAAYLIRSLARHL----IGHMKNSNDSNSIYKSMNAVLSIIIYSFKQIDLIN 736
            +    YL+ +   Q E+ + P V    +  I P KL     +  + +  +P  + +   + +Q   V +F  +R  LH    +  S  +      P  +D+E W  + LG+          NS          K    PL SI+  MSQ  +   +EYL +  ++  +T  +GRW+YALL+ L  PL  EA  LIR LAR       G     +D  S    +N  + ++   F+Q DL +
Sbjct:   23 RTPQEYLRRV---QIEAARCPDVV---IAQIDPKKLRKKQTVSISLSGCQPAPDGYSPSLRWQQQQVAQFSAVRQSLHKHRGHWRSQPLDSNVTMPSTEDEESWKKFCLGERLYSDLAAALNSESQHPGIDYIKVGFPPLLSIVSRMSQATVTSVLEYLVNWFEERNFTPELGRWLYALLACLEKPLLPEAHSLIRQLARRCSQIRAGVEHKEDDRVS---PLNLFICLVGRYFEQRDLAD 254
[Close]
BLAST of SMED30019845 vs. UniProt/SwissProt
Match: sp|Q9QZP1|GEMI2_RAT (Gem-associated protein 2 OS=Rattus norvegicus OX=10116 GN=Gemin2 PE=2 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 8.833e-11
Identity = 44/144 (30.56%), Postives = 68/144 (47.22%), Query Frame = 2 
Query:  359 PDIDDKEVWLGWVLGKNFNSGDNKGH--------------LEPLTSIICGMSQKQIIRSIEYLAD-LDDLPYTHLIGRWIYALLSVLSVPLNHEAAYLIRSLARHL--IGHMKNSNDSNSIYKSMNAVLSIIIYSFKQIDLINE 739
            P  +D+E W  + LG+   +    G                 PL SI+  M+Q  I   +EYL++   +  +T  +GRW YALL+ L  PL  EA  LIR LAR    +  +  S D   +  ++N ++ ++   F Q DL +E
Sbjct:  125 PKSEDEEGWKKFCLGERLCAEGATGPSTDESPGIDYVQVGFPPLLSIVSRMNQATITSVLEYLSNWFGERDFTPELGRWFYALLACLEKPLLPEAHSLIRQLARRCSEVRLLVGSKDDERV-PALNLLICLVSRYFDQRDLADE 267
[Close]
BLAST of SMED30019845 vs. UniProt/SwissProt
Match: sp|O14893|GEMI2_HUMAN (Gem-associated protein 2 OS=Homo sapiens OX=9606 GN=GEMIN2 PE=1 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 9.135e-11
Identity = 43/144 (29.86%), Postives = 70/144 (48.61%), Query Frame = 2 
Query:  359 PDIDDKEVWLGWVLGKNFNSGDNKGH--------------LEPLTSIICGMSQKQIIRSIEYLAD-LDDLPYTHLIGRWIYALLSVLSVPLNHEAAYLIRSLARHL--IGHMKNSNDSNSIYKSMNAVLSIIIYSFKQIDLINE 739
            P  +D+E W  + LG+   +    G                 PL SI+  M+Q  +   +EYL++   +  +T  +GRW+YALL+ L  PL  EA  LIR LAR    +  + +S D   +  ++N ++ ++   F Q DL +E
Sbjct:  136 PKSEDEEGWKKFCLGEKLCADGAVGPATNESPGIDYVQIGFPPLLSIVSRMNQATVTSVLEYLSNWFGERDFTPELGRWLYALLACLEKPLLPEAHSLIRQLARRCSEVRLLVDSKDDERV-PALNLLICLVSRYFDQRDLADE 278
[Close]
BLAST of SMED30019845 vs. TrEMBL
Match: G1KRR9 (Gem-associated protein 2 OS=Anolis carolinensis OX=28377 GN=GEMIN2 PE=3 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 1.890e-11
Identity = 56/175 (32.00%), Postives = 82/175 (46.86%), Query Frame = 2 
Query:  275 QIVNEFEHLR-----NILHTKTSNKSSLVIYNYPDIDDKEVWLGWVLGKNF--------NSGDNKG------HLEPLTSIICGMSQKQIIRSIEYLAD-LDDLPYTHLIGRWIYALLSVLSVPLNHEAAYLIRSLARHLIGHMKNSNDSNSIYKSMNAVLSIIIYSFKQIDLINE 739
            Q V +F  +R     N  H KT    S V    P  +D+E W  + LG+          +S DN+G         PL SI+  M+Q  +   +EYL + L +  +T  +GRW+YALL+ L  PL  EA  LIR LAR         N  +    ++N ++ ++   F Q DL +E
Sbjct:   91 QQVAQFSTIRQNVNKNRDHWKTQQLDSNVAI--PRSEDEEAWKKFCLGEKLYFETAVQVSSDDNEGIDYVQIGFPPLLSIVSRMNQATVTSVLEYLTNWLANKEFTPELGRWLYALLACLEKPLLPEAHSLIRQLARQCSEVRALENKDDEKVSALNLIICLVGRYFDQHDLADE 263
[Close]
BLAST of SMED30019845 vs. TrEMBL
Match: T1IKF6 (Uncharacterized protein OS=Strigamia maritima OX=126957 PE=4 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 2.103e-10
Identity = 38/134 (28.36%), Postives = 67/134 (50.00%), Query Frame = 2 
Query:  353 NYPDIDDKEVWLGWVLGKNFNSGD----NKGHLEPLTSIICGMSQKQIIRSIEY-LADLDDLPYTHLIGRWIYALLSVLSVPLNHEAAYLIRSLARHLIGHMKNSNDSNSIYKSMNAVLSIIIYSFKQIDLINE 739
            + P++DD   W     G    + +    N+  L PL SI+C MSQ  I + ++Y LA  +   +T   GRW +ALL+ L  PL  +A   +R+L R+        ++ ++    +N ++ ++   F Q DL ++
Sbjct:   30 DLPNMDDDVGWCRLCFGNVTTTAEKETINEDGLLPLVSIVCHMSQPLIEQVLDYHLAWFETTGFTEAQGRWFFALLACLEKPLTPDACSSLRTLVRYCANLRAAIDNDDARLPPLNLLICLVTRYFDQFDLADD 163
[Close]
BLAST of SMED30019845 vs. TrEMBL
Match: A0A087TD16 (Gem-associated protein 2 (Fragment) OS=Stegodyphus mimosarum OX=407821 GN=X975_11903 PE=4 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 3.593e-10
Identity = 40/103 (38.83%), Postives = 61/103 (59.22%), Query Frame = 2 
Query:  440 EPLTSIICGMSQKQIIRSIEYLAD-LDDLPYTHLIGRWIYALLSVLSVPLNHEAAYLIRSLARHLIGH-MKNSNDSNSIYKSMNAVLSIIIYSFKQIDLINEL 742
            EPL S++  ++Q+ +IR + Y  D L+D  +TH  G WIYALL  L  PL  +   L+RSL+R    H  K +N  ++  KS+N ++SII   F Q D+ ++ 
Sbjct:  166 EPLLSVVAHLNQRSVIRLLAYHIDWLEDGSFTHDQGVWIYALLVCLEKPLEPDTCALLRSLSRCCSTHRAKLNNPEDTALKSLNLIISIIANYFDQKDMSDKF 268
[Close]
BLAST of SMED30019845 vs. TrEMBL
Match: A0A5F8G5H2 (Gem-associated protein 2 OS=Monodelphis domestica OX=13616 GN=GEMIN2 PE=3 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 4.438e-10
Identity = 66/241 (27.39%), Postives = 110/241 (45.64%), Query Frame = 2 
Query:   89 KIHSSYLQMIIFYQNESKKLPCVTKRTVQNITPTKLGISYIILKNEAVLKPENEYHLSDVNFQI--VNEFEHLRNIL-----HTKTSNKSSLVIYNYPDIDDKEVWLGWVLGKNF--------NSGDNKG------HLEPLTSIICGMSQKQIIRSIEYLAD-LDDLPYTHLIGRWIYALLSVLSVPLNHEAAYLIRSLARHL--IGHMKNSNDSNSIYKSMNAVLSIIIYSFKQIDLINE 739
            +    YL+ +   Q E+ + P V    V +I P KL     +  + +  +P  E +   + +Q   V +F  +R  +     H K+ +  S V    P  +D+E W  + LG+           +G+N G         PL SI+  M+Q  I   +EYL +   +  +T  +GRW+YALL+ L  PL  EA  LIR LAR    +  +  S +   +  ++N ++ ++   F Q DL +E
Sbjct:   31 RTPQEYLKRV---QLEAAQCPDVV---VAHIDPKKLKRKQTVNVSLSGCQPAPEGYSPTLKWQQQQVAQFSTIRQSVNKHKNHWKSQHLDSNV--TMPKCEDEEGWKKFCLGERLYAEMSIATTTGENPGIDYVQIGFPPLLSIVSRMNQATITSVLEYLINWFGEKDFTPELGRWLYALLACLEKPLLPEAHSLIRQLARRCAEVRFLVESKEDERV-PALNLLICLVSRYFDQRDLADE 262
[Close]
BLAST of SMED30019845 vs. TrEMBL
Match: A0A1S2ZMS4 (Gem-associated protein 2 OS=Erinaceus europaeus OX=9365 GN=GEMIN2 PE=3 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 4.680e-10
Identity = 52/163 (31.90%), Postives = 81/163 (49.69%), Query Frame = 2 
Query:  302 RNILHTKTSNKSSLVIYNYPDIDDKEVWLGWVLGKNFNS-GD-------NKG------HLEPLTSIICGMSQKQIIRSIEYLAD-LDDLPYTHLIGRWIYALLSVLSVPLNHEAAYLIRSLARHL--IGHMKNSNDSNSIYKSMNAVLSIIIYSFKQIDLINE 739
            RN  H K+    S V  + P  +D+E W  + LG+   + GD       N G         PL SI+  M+Q  +   +EYL++   +  +T  +GRW+YALL+ L  PL  EA  LIR LAR    +  + +S D   +  ++N ++ ++   F Q DL +E
Sbjct:  108 RNRTHWKSQQLDSNV--SMPKPEDEEGWKKFCLGERLCAEGDVGPATSENPGIDYIQIGFPPLLSIVSRMNQATVTSVLEYLSNWFGERDFTAELGRWLYALLACLEKPLLPEAHSLIRQLARRCSEVRQLVDSKDDERV-PALNLLICLVSRYFDQHDLADE 267
[Close]
BLAST of SMED30019845 vs. Ensembl Cavefish
Match: gemin2 (gem nuclear organelle associated protein 2 [Source:NCBI gene;Acc:103028946])

HSP 1 Score: 63.5438 bits (153), Expect = 1.347e-11
Identity = 45/143 (31.47%), Postives = 71/143 (49.65%), Query Frame = 2 
Query:  359 PDIDDKEVWLGWVLGKNF-----NSGDN--------KGHLEPLTSIICGMSQKQIIRSIEYLAD-LDDLPYTHLIGRWIYALLSVLSVPLNHEAAYLIRSLARHLIGHMKN--SNDSNSIYKSMNAVLSIIIYSFKQIDLINE 739
            P  DD++ W  + LG+N      +S D         K    P  SI+  +SQ  +   +EYL    ++  +   +G+W+YALL+ L  PL  EA  LIR LAR       N  S D + +  ++N ++ I+   F+QIDL ++
Sbjct:  118 PKPDDEDGWRRFCLGENVYLNVTHSEDRVSPGLDYVKMGFPPFLSIVSKLSQSTVSAVLEYLISWFEERDFVPQLGQWLYALLACLEKPLLPEAHSLIRQLARRCSAVRANLESKDDDRL-SALNLLICIVARYFEQIDLADK 259
[Close]
BLAST of SMED30019845 vs. Ensembl Sea Lamprey
Match: gemin2 (gem (nuclear organelle) associated protein 2 [Source:ZFIN;Acc:ZDB-GENE-030131-3756])

HSP 1 Score: 50.0618 bits (118), Expect = 1.254e-7
Identity = 55/223 (24.66%), Postives = 92/223 (41.26%), Query Frame = 2 
Query:  134 ESKKLPCVTKRTVQNITPTKLG------ISYIILKNEAVLKPENEYHLSDVNFQIVNE--FEHLRNILHTKT------SNKSSLVIYNYPDIDDKEVWLGWVLG-KNFNSG---------------DNKGHLE------------PLTSIICGMSQKQIIRSIEYLAD-LDDLPYTHLIGRWIYALLSVLSVPLNHEAAYLIRSLARH---LIGHMKNSNDSN 664
            E+ + P V    V NI P KL       +S +  +    L P        V +Q+  +  F  +R    + T      S+  S  +   P+ +D+E W  + LG + + S                D+    E            PL S++  + Q  +I  +EYL    ++  ++  +GRWIYALL+ +  PL  EA  L+R +AR+   L   +++  DS 
Sbjct:   35 EAARCPEVA---VANIDPEKLKRKQPVKVSNLSFQPCGCL-PAPRGFAPSVEWQMQQQAAFSAMRQRTSSSTDTSARVSSARSCNVTTQPEAEDEEGWKRFCLGERRYGSRAQSEPPPAAAAAGVHDDPADGEELDLDFLKIGTPPLLSVVGNLGQATLIVVLEYLTVWFEERGFSEELGRWIYALLACIEKPLLPEAHSLLRQIARNCASLRASLESPEDSR 253
[Close]
BLAST of SMED30019845 vs. Ensembl Nematostella
Match: EDO32758 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SU44])

HSP 1 Score: 51.2174 bits (121), Expect = 8.683e-8
Identity = 52/253 (20.55%), Postives = 102/253 (40.32%), Query Frame = 2 
Query:   89 KIHSSYLQMIIFYQNESKKLPCVTKRTVQNITPTKLGISYIILKNE---AVLKPENEYHLSDVNFQIVNEFEHLRNILHTKTSNKSSLVIYN--YPDIDDKEVWLGWVLGK---------------------------NFNSGDNKGHLEPLTSIICGMSQKQIIRSIEY-LADLDDLPYTHLIGRWIYALLSVLSVPLNHEAAYLIRSLARHL----IGHMKNSNDSNSIYKSMNAVLSIIIYSFKQIDLIN 736
            K+ ++ L+ +   Q E+++ P V    + ++T  + G    +  N+       P       +   Q   +F  +R  L    +  +SL   N  +P  +D+  W  + LG+                           NF     +    P  +++  ++Q  + R +E+    +  L Y+H  GRWIY+LL  L  PL  +    +R+LAR      +  M  +  +  +  S+N +++++   F Q DL +
Sbjct:   22 KLPTTGLEYLRRVQQEAERCPEVV---IADLTNKEFGKKQTVKINQDSGCAPAPTGAAPSLEWQQQQAADFADVRQCLANYKTKANSLTDANISWPSKNDEIQWCRFCLGREAAIHICKAFGYPDSRLTHPQQMPEMDNFTEQQWENGSPPTLTMMVKLNQTLVQRLLEWHTLWMSVLGYSHDQGRWIYSLLVCLEKPLLADCTATLRTLARQCTECRLTQMPMNEKNVELVTSLNLIIALVSRYFGQTDLAD 271
[Close]
BLAST of SMED30019845 vs. Ensembl Medaka
Match: gemin2 (gem nuclear organelle associated protein 2 [Source:NCBI gene;Acc:101158153])

HSP 1 Score: 47.7506 bits (112), Expect = 2.891e-6
Identity = 42/162 (25.93%), Postives = 76/162 (46.91%), Query Frame = 2 
Query:  275 QIVNEFEHLRNILHTKTSNKSSLVIYN---YPDIDDKEVWLGWVLGKNFNSGDNKGHLEPLTSIICGMSQKQIIRSIEYLAD-LDDLPYTHLIGRWIYALLSVLSVPLNHEAAYLIRSLAR---HLIGHMKNSNDSNSIYKSMNAVLSIIIYSFKQIDLINE 739
            Q V+ F  +R  +     + SS  + N    P++ D+E W  + LG+            P  +I+C ++Q   +  ++ L    ++      +G W+YALL+ L  PL  EA   IR LAR    L   +++ +D    +  +N ++ +  Y F+Q DL ++
Sbjct:   89 QQVSNFSDVRRSITKNRKHWSSQTLDNNVRMPNLTDEEGWKKFCLGE--------VGFPPFLTIVCRLNQSTALMVLDVLISWFEEHELVPQLGCWLYALLACLEKPLLPEAHSSIRQLARRCAQLRSTLESQDDDRLAH--LNLLICLAAY-FEQNDLADQ 239
[Close]
BLAST of SMED30019845 vs. Planmine SMEST
Match: SMESG000032442.1 (SMESG000032442.1)

HSP 1 Score: 416.001 bits (1068), Expect = 5.527e-149
Identity = 203/205 (99.02%), Postives = 205/205 (100.00%), Query Frame = 2 
Query:  128 QNESKKLPCVTKRTVQNITPTKLGISYIILKNEAVLKPENEYHLSDVNFQIVNEFEHLRNILHTKTSNKSSLVIYNYPDIDDKEVWLGWVLGKNFNSGDNKGHLEPLTSIICGMSQKQIIRSIEYLADLDDLPYTHLIGRWIYALLSVLSVPLNHEAAYLIRSLARHLIGHMKNSNDSNSIYKSMNAVLSIIIYSFKQIDLINEL 742
            +NESKKLPCVTKRTVQNITPTKLGISYIILKNEAVLKPENEYHLSDVNFQIVNEFEHLRNILHTKTSNKSSLVIYNYPDIDDKEVWLGWVLGKNFNSGDNKGHLEPLTSIICGMSQKQIIRSIEYLADLDDLPYTHLIGRWIYALLSVLSVPLNHEAAYLIR+LARHLIGHMKNSNDSNSIYKSMNAVLSIIIYSFKQIDLINEL
Sbjct:   42 RNESKKLPCVTKRTVQNITPTKLGISYIILKNEAVLKPENEYHLSDVNFQIVNEFEHLRNILHTKTSNKSSLVIYNYPDIDDKEVWLGWVLGKNFNSGDNKGHLEPLTSIICGMSQKQIIRSIEYLADLDDLPYTHLIGRWIYALLSVLSVPLNHEAAYLIRTLARHLIGHMKNSNDSNSIYKSMNAVLSIIIYSFKQIDLINEL 246

HSP 2 Score: 83.1889 bits (204), Expect = 6.665e-19
Identity = 40/40 (100.00%), Postives = 40/40 (100.00%), Query Frame = 1 
Query:   10 MSDNFSSDELNDNKFFHVEGEYNELTKNSQFLSPNDYLLS 129
            MSDNFSSDELNDNKFFHVEGEYNELTKNSQFLSPNDYLLS
Sbjct:    1 MSDNFSSDELNDNKFFHVEGEYNELTKNSQFLSPNDYLLS 40
[Close]
BLAST of SMED30019845 vs. Planmine SMEST
Match: SMESG000032451.1 (SMESG000032451.1)

HSP 1 Score: 412.92 bits (1060), Expect = 4.766e-148
Identity = 203/204 (99.51%), Postives = 204/204 (100.00%), Query Frame = 2 
Query:  131 NESKKLPCVTKRTVQNITPTKLGISYIILKNEAVLKPENEYHLSDVNFQIVNEFEHLRNILHTKTSNKSSLVIYNYPDIDDKEVWLGWVLGKNFNSGDNKGHLEPLTSIICGMSQKQIIRSIEYLADLDDLPYTHLIGRWIYALLSVLSVPLNHEAAYLIRSLARHLIGHMKNSNDSNSIYKSMNAVLSIIIYSFKQIDLINEL 742
            NESKKLPCVTKRTVQNITPTKLGISYIILKNEAVLKPENEYHLSDVNFQIVNEFE+LRNILHTKTSNKSSLVIYNYPDIDDKEVWLGWVLGKNFNSGDNKGHLEPLTSIICGMSQKQIIRSIEYLADLDDLPYTHLIGRWIYALLSVLSVPLNHEAAYLIRSLARHLIGHMKNSNDSNSIYKSMNAVLSIIIYSFKQIDLINEL
Sbjct:   34 NESKKLPCVTKRTVQNITPTKLGISYIILKNEAVLKPENEYHLSDVNFQIVNEFENLRNILHTKTSNKSSLVIYNYPDIDDKEVWLGWVLGKNFNSGDNKGHLEPLTSIICGMSQKQIIRSIEYLADLDDLPYTHLIGRWIYALLSVLSVPLNHEAAYLIRSLARHLIGHMKNSNDSNSIYKSMNAVLSIIIYSFKQIDLINEL 237
[Close]
BLAST of SMED30019845 vs. Planmine SMEST
Match: SMESG000032451.1 (SMESG000032451.1)

HSP 1 Score: 413.69 bits (1062), Expect = 1.234e-147
Identity = 203/205 (99.02%), Postives = 205/205 (100.00%), Query Frame = 2 
Query:  128 QNESKKLPCVTKRTVQNITPTKLGISYIILKNEAVLKPENEYHLSDVNFQIVNEFEHLRNILHTKTSNKSSLVIYNYPDIDDKEVWLGWVLGKNFNSGDNKGHLEPLTSIICGMSQKQIIRSIEYLADLDDLPYTHLIGRWIYALLSVLSVPLNHEAAYLIRSLARHLIGHMKNSNDSNSIYKSMNAVLSIIIYSFKQIDLINEL 742
            +NESKKLPCVTKRTVQNITPTKLGISYIILKNEAVLKPENEYHLSDVNFQIVNEFE+LRNILHTKTSNKSSLVIYNYPDIDDKEVWLGWVLGKNFNSGDNKGHLEPLTSIICGMSQKQIIRSIEYLADLDDLPYTHLIGRWIYALLSVLSVPLNHEAAYLIRSLARHLIGHMKNSNDSNSIYKSMNAVLSIIIYSFKQIDLINEL
Sbjct:   74 RNESKKLPCVTKRTVQNITPTKLGISYIILKNEAVLKPENEYHLSDVNFQIVNEFENLRNILHTKTSNKSSLVIYNYPDIDDKEVWLGWVLGKNFNSGDNKGHLEPLTSIICGMSQKQIIRSIEYLADLDDLPYTHLIGRWIYALLSVLSVPLNHEAAYLIRSLARHLIGHMKNSNDSNSIYKSMNAVLSIIIYSFKQIDLINEL 278

HSP 2 Score: 79.337 bits (194), Expect = 2.771e-17
Identity = 38/38 (100.00%), Postives = 38/38 (100.00%), Query Frame = 1 
Query:   16 DNFSSDELNDNKFFHVEGEYNELTKNSQFLSPNDYLLS 129
            DNFSSDELNDNKFFHVEGEYNELTKNSQFLSPNDYLLS
Sbjct:   35 DNFSSDELNDNKFFHVEGEYNELTKNSQFLSPNDYLLS 72
[Close]
BLAST of SMED30019845 vs. Planmine SMEST
Match: SMESG000032442.1 (SMESG000032442.1)

HSP 1 Score: 399.438 bits (1025), Expect = 1.248e-142
Identity = 198/205 (96.59%), Postives = 200/205 (97.56%), Query Frame = 2 
Query:  128 QNESKKLPCVTKRTVQNITPTKLGISYIILKNEAVLKPENEYHLSDVNFQIVNEFEHLRNILHTKTSNKSSLVIYNYPDIDDKEVWLGWVLGKNFNSGDNKGHLEPLTSIICGMSQKQIIRSIEYLADLDDLPYTHLIGRWIYALLSVLSVPLNHEAAYLIRSLARHLIGHMKNSNDSNSIYKSMNAVLSIIIYSFKQIDLINEL 742
            +NESKKLPCVTKRTVQNITPTKLGISYIILKNEAVLKPENEYHLSDVNFQIVNEFEHLRNILHTKTSNKSSL     PDIDDKEVWLGWVLGKNFNSGDNKGHLEPLTSIICGMSQKQIIRSIEYLADLDDLPYTHLIGRWIYALLSVLSVPLNHEAAYLIR+LARHLIGHMKNSNDSNSIYKSMNAVLSIIIYSFKQIDLINEL
Sbjct:   42 RNESKKLPCVTKRTVQNITPTKLGISYIILKNEAVLKPENEYHLSDVNFQIVNEFEHLRNILHTKTSNKSSL-----PDIDDKEVWLGWVLGKNFNSGDNKGHLEPLTSIICGMSQKQIIRSIEYLADLDDLPYTHLIGRWIYALLSVLSVPLNHEAAYLIRTLARHLIGHMKNSNDSNSIYKSMNAVLSIIIYSFKQIDLINEL 241

HSP 2 Score: 83.1889 bits (204), Expect = 6.186e-19
Identity = 40/40 (100.00%), Postives = 40/40 (100.00%), Query Frame = 1 
Query:   10 MSDNFSSDELNDNKFFHVEGEYNELTKNSQFLSPNDYLLS 129
            MSDNFSSDELNDNKFFHVEGEYNELTKNSQFLSPNDYLLS
Sbjct:    1 MSDNFSSDELNDNKFFHVEGEYNELTKNSQFLSPNDYLLS 40
The following BLAST results are available for this feature:
BLAST of SMED30019845 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 4
Match NameE-valueIdentityDescription
GEMIN21.902e-1129.86gem nuclear organelle associated protein 2 [Source... [more]
GEMIN23.653e-1031.06gem nuclear organelle associated protein 2 [Source... [more]
GEMIN25.032e-933.33gem nuclear organelle associated protein 2 [Source... [more]
GEMIN26.115e-933.33gem nuclear organelle associated protein 2 [Source... [more]
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BLAST of SMED30019845 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019845 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019845 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
gemin22.299e-1127.31gem (nuclear organelle) associated protein 2 [Sour... [more]
gemin22.371e-1127.31gem (nuclear organelle) associated protein 2 [Sour... [more]
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BLAST of SMED30019845 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ndufaf15.422e-1326.67NADH:ubiquinone oxidoreductase complex assembly fa... [more]
ndufaf13.497e-1228.86NADH:ubiquinone oxidoreductase complex assembly fa... [more]
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BLAST of SMED30019845 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Gemin21.930e-1227.20gem nuclear organelle associated protein 2 [Source... [more]
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BLAST of SMED30019845 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 4
Match NameE-valueIdentityDescription
sp|Q9CQQ4|GEMI2_MOUSE1.350e-1127.20Gem-associated protein 2 OS=Mus musculus OX=10090 ... [more]
sp|O42260|GEMI2_XENLA4.666e-1126.56Gem-associated protein 2 OS=Xenopus laevis OX=8355... [more]
sp|Q9QZP1|GEMI2_RAT8.833e-1130.56Gem-associated protein 2 OS=Rattus norvegicus OX=1... [more]
sp|O14893|GEMI2_HUMAN9.135e-1129.86Gem-associated protein 2 OS=Homo sapiens OX=9606 G... [more]
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BLAST of SMED30019845 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
G1KRR91.890e-1132.00Gem-associated protein 2 OS=Anolis carolinensis OX... [more]
T1IKF62.103e-1028.36Uncharacterized protein OS=Strigamia maritima OX=1... [more]
A0A087TD163.593e-1038.83Gem-associated protein 2 (Fragment) OS=Stegodyphus... [more]
A0A5F8G5H24.438e-1027.39Gem-associated protein 2 OS=Monodelphis domestica ... [more]
A0A1S2ZMS44.680e-1031.90Gem-associated protein 2 OS=Erinaceus europaeus OX... [more]
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BLAST of SMED30019845 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
gemin21.347e-1131.47gem nuclear organelle associated protein 2 [Source... [more]
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BLAST of SMED30019845 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
gemin21.254e-724.66gem (nuclear organelle) associated protein 2 [Sour... [more]
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BLAST of SMED30019845 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019845 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO327588.683e-820.55Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SMED30019845 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
gemin22.891e-625.93gem nuclear organelle associated protein 2 [Source... [more]
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BLAST of SMED30019845 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4
Match NameE-valueIdentityDescription
SMESG000032442.15.527e-14999.02SMESG000032442.1[more]
SMESG000032451.14.766e-14899.51SMESG000032451.1[more]
SMESG000032451.11.234e-14799.02SMESG000032451.1[more]
SMESG000032442.11.248e-14296.59SMESG000032442.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30019845 ID=SMED30019845|Name=SMED30019845|organism=Schmidtea mediterranea sexual|type=transcript|length=748bp
TATTTAATTATGTCAGACAACTTTTCTAGTGATGAATTAAATGATAATAA
GTTTTTCCATGTTGAAGGCGAATATAATGAATTGACTAAAAATTCACAGT
TCCTATCTCCAAATGATTATCTTCTATCAAAATGAATCCAAAAAATTACC
ATGTGTCACTAAAAGAACTGTTCAAAACATCACTCCAACAAAATTAGGAA
TATCCTATATAATTCTAAAAAATGAAGCAGTTTTGAAACCCGAAAATGAG
TATCACCTATCAGATGTAAACTTTCAAATTGTAAATGAATTTGAACATTT
AAGAAACATATTACATACAAAGACATCTAATAAATCATCACTTGTAATAT
ATAACTATCCAGATATTGATGACAAAGAAGTTTGGTTAGGATGGGTTTTA
GGAAAGAATTTCAATTCTGGGGATAACAAGGGACACTTGGAACCCCTTAC
TTCAATTATTTGTGGAATGAGTCAGAAACAAATTATACGATCTATTGAAT
ATTTGGCTGATCTTGATGACTTACCGTATACTCACTTAATAGGACGGTGG
ATTTATGCTCTTCTTTCGGTGTTATCTGTTCCCTTGAATCACGAAGCAGC
CTATTTAATCCGCTCACTCGCTCGACATTTGATTGGACACATGAAAAATT
CGAATGATTCAAATTCCATTTATAAATCCATGAATGCCGTGCTCAGTATT
ATTATTTATAGTTTTAAGCAAATCGATTTAATAAATGAATTATGATTG
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protein sequence of SMED30019845-orf-1

>SMED30019845-orf-1 ID=SMED30019845-orf-1|Name=SMED30019845-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=211bp
MIIFYQNESKKLPCVTKRTVQNITPTKLGISYIILKNEAVLKPENEYHLS
DVNFQIVNEFEHLRNILHTKTSNKSSLVIYNYPDIDDKEVWLGWVLGKNF
NSGDNKGHLEPLTSIICGMSQKQIIRSIEYLADLDDLPYTHLIGRWIYAL
LSVLSVPLNHEAAYLIRSLARHLIGHMKNSNDSNSIYKSMNAVLSIIIYS
FKQIDLINEL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR035426Gemin2/Brr1
IPR017364GEMIN2
Vocabulary: biological process
TermDefinition
GO:0000245spliceosomal complex assembly
GO:0000387spliceosomal snRNP assembly
Vocabulary: cellular component
TermDefinition
GO:0005681spliceosomal complex
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR035426Gemin2/Brr1PFAMPF04938SIP1coord: 37..203
e-value: 3.1E-20
score: 72.8
NoneNo IPR availableGENE3DG3DSA:1.20.58.1070coord: 50..209
e-value: 6.2E-23
score: 83.7
NoneNo IPR availablePANTHERPTHR12794GEMIN2coord: 8..206
NoneNo IPR availablePANTHERPTHR12794:SF0GEM-ASSOCIATED PROTEIN 2coord: 8..206
IPR017364Gem-associated protein 2PIRSFPIRSF038038Gemin-2coord: 6..210
e-value: 2.1E-17
score: 61.0