Dimer_Tnp_hAT domain-containing protein

Overview
NameDimer_Tnp_hAT domain-containing protein
Smed IDSMED30019833
Length (bp)1269
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Dimer_Tnp_hAT domain-containing protein (SMED30019833) t-SNE clustered cells

Violin plots show distribution of expression levels for Dimer_Tnp_hAT domain-containing protein (SMED30019833) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Dimer_Tnp_hAT domain-containing protein (SMED30019833) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Dimer_Tnp_hAT domain-containing protein (SMED30019833) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30019833

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pharynxSMED30019833h1SMcG0021727 dd_Smed_v4_2402_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30019833h1SMcG0021727 dd_Smed_v4_2402_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30019833h1SMcG0021727 dd_Smed_v4_2402_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30019833h1SMcG0021727 Contig35498newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30019833h1SMcG0021727 Contig35498uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Dimer_Tnp_hAT domain-containing protein vs. TrEMBL
Match: A0A4C1V2H5 (Uncharacterized protein OS=Eumeta variegata OX=151549 GN=EVAR_82851_1 PE=4 SV=1)

HSP 1 Score: 136.732 bits (343), Expect = 1.145e-41
Identity = 96/230 (41.74%), Postives = 135/230 (58.70%), Query Frame = 2 
Query:  314 ISILFRLST-KNDDILQKYVKTEFG*ELMLILDSKTRWNSLLTILERFLKLKSAIQKALIDIKSSYPFLADKFNLLSETVATITPIKYALEAICRQDANLLTADATLKLLICTLGKINCVLANDMKNALEFRIKKRRT-NLSGX------XXXXXXXXXXXXXXPTDLFPALKKITIKRLLLNMVQRLFISESNNEND*LRQQHSDENVSMKVSILEETENDSDTIKQKM 979
            + I F+ S  KN+ +LQKYVK E G EL L+LD KTRWNSLLT+LERF  LKS IQKALID+         +F L+++ V  +TPIK  +EAICR+DANL TADA L  LI  L   N  LA  +K  L  RIK+RR+ NL+G+       +    + L S     +LFP L +  +++ + ++V+RL      N  D   ++   E+++  V +  ET     T+K+K+
Sbjct:  197 VVIYFKRSPLKNETVLQKYVKIEHGKELSLLLDCKTRWNSLLTMLERFYLLKSCIQKALIDLNHPICLEDVEFELVNKIVDVLTPIKLTVEAICRRDANLCTADAALNFLIKQLSSKNSALAKKIKTCLVDRIKQRRSDNLTGVLNYLQNPRKDEENQLLSELEWHNLFPVLTQALLRKQIKSIVKRL------NSRDDTEEEERSESLT-DVELRPETSQPQLTLKEKL 419

HSP 2 Score: 63.929 bits (154), Expect = 1.145e-41
Identity = 47/121 (38.84%), Postives = 64/121 (52.89%), Query Frame = 1 
Query:    1 F*NLGLACVVGR-----CVDLF-QKLSIFNISMEKDVVCITID---VMKEVGKLVNASQHLCFAHGIQLAVLSVLYRNDTSTEVELLADLPEN---------EHSSDEDSADAGLGAGNQN 309
            F NLGL  + G      C+ +  ++LS F+IS+E D+V I  D   VM +VG+LV A Q LC AHGI LAV  VLY+         L D P +         +H  D D +++GL   +QN
Sbjct:   56 FWNLGLVPIYGNMTAENCLRILNERLSKFDISLETDIVAIVTDGPNVMIKVGRLVCAEQQLCLAHGINLAVCDVLYKKHN-----YLQDSPTSDDMDDNNDDDHLEDLDCSESGLILMSQN 171
[Close]
BLAST of Dimer_Tnp_hAT domain-containing protein vs. TrEMBL
Match: A0A4C1XQA5 (Uncharacterized protein OS=Eumeta variegata OX=151549 GN=EVAR_98391_1 PE=4 SV=1)

HSP 1 Score: 135.191 bits (339), Expect = 2.398e-40
Identity = 78/143 (54.55%), Postives = 96/143 (67.13%), Query Frame = 2 
Query:  314 ISILFRLST-KNDDILQKYVKTEFG*ELMLILDSKTRWNSLLTILERFLKLKSAIQKALIDIKSSYPFLADKFNLLSETVATITPIKYALEAICRQDANLLTADATLKLLICTLGKINCVLANDMKNALEFRIKKRRT-NLSG 736
            + I F+ S  KN+ +LQKYVK E G EL L+LD KTRWNSLLT+LERF  LKS IQKALID+         +F L+++ V  +TPIK  +EAICR+DANL TADA L  LI  L   N  LA  +K  L  RIK+RR+ NL+G
Sbjct:  130 VVIYFKRSPLKNETVLQKYVKIEHGKELSLLLDCKTRWNSLLTMLERFYLLKSCIQKALIDLNHPICLEDVEFELVNKIVDVLTPIKLTVEAICRRDANLCTADAALNFLIKQLSSKNSALAKKIKTCLVDRIKQRRSDNLTG 272

HSP 2 Score: 60.8474 bits (146), Expect = 2.398e-40
Identity = 41/100 (41.00%), Postives = 55/100 (55.00%), Query Frame = 1 
Query:   46 LFQKLSIFNISMEKDVVCITID---VMKEVGKLVNASQHLCFAHGIQLAVLSVLYRNDTSTEVELLADLPEN---------EHSSDEDSADAGLGAGNQN 309
            L ++LS F+IS+E D+V I  D   VM +VG+LV A Q LC AHGI LAV  VLY+         L D P +         +H  D D +++GL   +QN
Sbjct:   10 LNERLSKFDISLETDIVAIVTDGPNVMIKVGRLVCAEQQLCLAHGINLAVCDVLYKKHN-----YLQDSPTSDDMDDNNDDDHLEDLDCSESGLILMSQN 104
[Close]
BLAST of Dimer_Tnp_hAT domain-containing protein vs. TrEMBL
Match: A0A4C1X5B5 (Uncharacterized protein OS=Eumeta variegata OX=151549 GN=EVAR_51242_1 PE=4 SV=1)

HSP 1 Score: 133.65 bits (335), Expect = 8.222e-40
Identity = 78/143 (54.55%), Postives = 96/143 (67.13%), Query Frame = 2 
Query:  314 ISILFRLST-KNDDILQKYVKTEFG*ELMLILDSKTRWNSLLTILERFLKLKSAIQKALIDIKSSYPFLADKFNLLSETVATITPIKYALEAICRQDANLLTADATLKLLICTLGKINCVLANDMKNALEFRIKKRRT-NLSG 736
            + I F+ S  KN+ +LQKYVK E G EL L+LD KTRWNSLLT+LERF  LKS IQKALID+         +F L+++ V  +TPIK  +EAICR+DANL TADA L  LI  L   N  LA  +K  L  RIK+RR+ NL+G
Sbjct:  130 VVIYFKRSPLKNETVLQKYVKIEHGKELSLLLDCKTRWNSLLTMLERFYLLKSCIQKALIDLNHPICLEDVEFELVNKIVDVLTPIKLTVEAICRRDANLCTADAALNFLIKQLSSKNSALAKKIKTCLVDRIKQRRSDNLTG 272

HSP 2 Score: 60.8474 bits (146), Expect = 8.222e-40
Identity = 41/100 (41.00%), Postives = 55/100 (55.00%), Query Frame = 1 
Query:   46 LFQKLSIFNISMEKDVVCITID---VMKEVGKLVNASQHLCFAHGIQLAVLSVLYRNDTSTEVELLADLPEN---------EHSSDEDSADAGLGAGNQN 309
            L ++LS F+IS+E D+V I  D   VM +VG+LV A Q LC AHGI LAV  VLY+         L D P +         +H  D D +++GL   +QN
Sbjct:   10 LNERLSKFDISLETDIVAIVTDGPNVMIKVGRLVCAEQQLCLAHGINLAVCDVLYKKHN-----YLQDSPTSDDMDDNNDDDHLEDLDCSESGLILMSQN 104
[Close]
BLAST of Dimer_Tnp_hAT domain-containing protein vs. TrEMBL
Match: A0A4C1X383 (Uncharacterized protein OS=Eumeta variegata OX=151549 GN=EVAR_86333_1 PE=4 SV=1)

HSP 1 Score: 123.25 bits (308), Expect = 8.909e-37
Identity = 75/143 (52.45%), Postives = 94/143 (65.73%), Query Frame = 2 
Query:  314 ISILFRLST-KNDDILQKYVKTEFG*ELMLILDSKTRWNSLLTILERFLKLKSAIQKALIDIKSSYPFLADKFNLLSETVATITPIKYALEAICRQDANLLTADATLKLLICTLGKINCVLANDMKNALEFRIKKRRT-NLSG 736
            + I F+ S  KN+ +LQKYV+ E G EL L+LD KTRWNSLLT+LERF  LKS IQKALID+         +F L+++ V  +TPIK  +EAICR+DANL TADA L  LI  L      L   +K  L  RIK+RR+ NL+G
Sbjct:  130 VVIYFKRSPLKNETVLQKYVRIEHGEELSLLLDCKTRWNSLLTMLERFYLLKSCIQKALIDLNHPICLEDVEFELVNKIVDVLTPIKLTVEAICRRDANLCTADAALNFLIKQLSSKTQHLPK-IKTCLVDRIKQRRSDNLTG 271

HSP 2 Score: 61.2326 bits (147), Expect = 8.909e-37
Identity = 40/95 (42.11%), Postives = 58/95 (61.05%), Query Frame = 1 
Query:   46 LFQKLSIFNISMEKDVVCITID---VMKEVGKLVNASQHLCFAHGIQLAVLSVLYRNDT----STEVELLADLPENEHSSDEDSADAGLGAGNQN 309
            L ++LS F+IS+E D+V I  D   VM +VG+LV A Q LC AHGI LAV  VLY+       S  ++ + D  +++H  D D +++GL   +QN
Sbjct:   10 LNERLSKFDISLETDIVAIVTDGPNVMIKVGRLVCAEQQLCLAHGINLAVCDVLYKKHNYLQDSPTLDDMDDNNDDDHLEDLDCSESGLILMSQN 104
[Close]
BLAST of Dimer_Tnp_hAT domain-containing protein vs. TrEMBL
Match: A0A226DDR7 (Putative AC9 transposase OS=Folsomia candida OX=158441 GN=Fcan01_16136 PE=4 SV=1)

HSP 1 Score: 116.701 bits (291), Expect = 2.355e-33
Identity = 58/143 (40.56%), Postives = 97/143 (67.83%), Query Frame = 2 
Query:  305 RTTISILFRLSTKNDDILQKYVKTEFG*ELMLILDSKTRWNSLLTILERFLKLKSAIQKALIDIKSSYPFLADKFNLLSETVATITPIKYALEAICRQDANLLTADATLKLLICTLGKINCVLANDMKNALEFRIKKRRTNLS 733
            R+T+  +FR S K ++ILQ YVK+EF  EL LILD +TRWNSL  ++ERF  +++ I+KA ID++ +  F   +FN + + +  + PI+ A+E IC +++NL+T DA L L+I  L      ++ ++ ++++FR+ +RRT L+
Sbjct:  348 RSTVK-MFRRSPKKNEILQNYVKSEFQKELNLILDCRTRWNSLAAMIERFHLIRTCIKKAFIDLRVATNFEEQEFNYIFQVMEALKPIRAAVEVICNRESNLITTDAALNLVISKLRAQKSSVSLELASSIKFRVMERRTELT 489

HSP 2 Score: 56.225 bits (134), Expect = 2.355e-33
Identity = 38/105 (36.19%), Postives = 61/105 (58.10%), Query Frame = 1 
Query:    1 F*NLGLACVVG-----RCVDLF-QKLSIFNISMEKDVVCITID---VMKEVGKLVNAS-QHLCFAHGIQLAVLSVLY-RNDTSTEVELLADLPENEHSSDEDSAD 282
            F NLGL  ++G      C+ L  +K++   +++ KD+V I  D   VMK++G+    + Q LC AHGIQLAVL VLY + +   + + L  + EN   SD++ ++
Sbjct:  205 FWNLGLVPILGSATAENCLQLVDEKITSVGLNLHKDIVAIITDGSSVMKKIGRDAAPTFQQLCLAHGIQLAVLDVLYAKGENVGDTDNLEAIDENSSFSDDEGSE 309
[Close]
BLAST of Dimer_Tnp_hAT domain-containing protein vs. Planmine SMEST
Match: SMESG000061313.1 (SMESG000061313.1)

HSP 1 Score: 87.8113 bits (216), Expect = 4.206e-23
Identity = 47/134 (35.07%), Postives = 77/134 (57.46%), Query Frame = 2 
Query:  323 LFRLSTKNDDILQKYVKTEFG*ELMLILDSKTRWNSLLTILERFLKLKSAIQKALIDIKSSYPFLADKFNLLSETVATITPIKYALEAICRQDANLLTADATLKLLICTLGKINCVLANDMKNALEFRIKKRRT 724
             FR S   +DILQ+YV+ + G +L LILD +TRWNSL+ ++ER   L+  I+ AL +I+    +    FN+L+  +  + P + A++ + +  + LLTA+  L  L   + + N  L     N+L+ RI +RR 
Sbjct:  287 FFRKSPVRNDILQRYVEDKEGKKLKLILDCRTRWNSLIPMMERLFLLQVCIKHALEEIRRDDLYNEKDFNILANLIEILKPTELAVKELSKHTSTLLTAEGVLTFLFTQMKQNNTALGKKFYNSLKIRISERRN 420

HSP 2 Score: 39.2762 bits (90), Expect = 4.206e-23
Identity = 31/115 (26.96%), Postives = 54/115 (46.96%), Query Frame = 1 
Query:   10 LGLACVVGRCVDLFQ-------KLSIFNISMEKDVVCITID---VMKEVGKLVNASQHLCFAHGIQLAVLSVLYRNDTSTEVELLADLPENEHS-SDEDSADAGLGAGNQNYNFN 321
            LGL  + G C D +Q       KL  + I + ++++  T D   VMK+  +L   +   C  H I LAV+   Y  +      ++ ++ ++E++  DED       AG+ N N +
Sbjct:  150 LGLIAIEGSC-DAYQIHSLVEQKLMDYGIKL-RNILASTQDGASVMKKYAQLNKITNQFCINHAIHLAVVETFYNKNN-----VIDEISDSENADGDEDENQFNSFAGDHNLNID 257
[Close]
BLAST of Dimer_Tnp_hAT domain-containing protein vs. Planmine SMEST
Match: SMESG000022344.1 (SMESG000022344.1)

HSP 1 Score: 79.337 bits (194), Expect = 1.290e-20
Identity = 43/134 (32.09%), Postives = 74/134 (55.22%), Query Frame = 2 
Query:  323 LFRLSTKNDDILQKYVKTEFG*ELMLILDSKTRWNSLLTILERFLKLKSAIQKALIDIKSSYPFLADKFNLLSETVATITPIKYALEAICRQDANLLTADATLKLLICTLGKINCVLANDMKNALEFRIKKRRT 724
             FR S   +DILQ+Y + + G +  LILD +TRWNSL+ ++ER + L+  I+ AL +I     +    FN+L   +  + P ++A++ + +  + L+TA+  L  L   + + N  L     N+L+  I +RR 
Sbjct:  291 FFRKSPVRNDILQRYDEDKEGKKFKLILDCRTRWNSLIPMMERLVLLQVCIKHALEEIGCDDLYNEKDFNILENLIEILKPTEFAVKELSKHTSTLITAERVLTFLFTQMKQNNTALGKKFYNSLKIGISERRN 424

HSP 2 Score: 39.2762 bits (90), Expect = 1.290e-20
Identity = 31/115 (26.96%), Postives = 54/115 (46.96%), Query Frame = 1 
Query:   10 LGLACVVGRCVDLFQ-------KLSIFNISMEKDVVCITID---VMKEVGKLVNASQHLCFAHGIQLAVLSVLYRNDTSTEVELLADLPENEHS-SDEDSADAGLGAGNQNYNFN 321
            LGL  + G C D +Q       KL  ++I + ++++  T D   VMK+  +L       C  H I LAV+   Y  +      ++ ++ ++E++  DED       AG+ N N +
Sbjct:  154 LGLIAIEGSC-DAYQIHSLVEQKLMYYDIKL-RNILASTQDGASVMKKYAQLNKIENQFCINHAIHLAVVETFYNKNN-----VIDEISDSENADGDEDENQFNSFAGDHNLNID 261

HSP 3 Score: 49.2914 bits (116), Expect = 4.195e-6
Identity = 25/51 (49.02%), Postives = 32/51 (62.75%), Query Frame = 1 
Query: 1075 RSKWLDLCYNTLK*IPPTSEKSESAFSVSGNFCTKLRSRLSDETIDNLKFF 1227
            +SK +   +N L  I PTS  SE  FS++GNFCTKLRS +  E +D L F 
Sbjct:  535 KSKTVQNLFNALASIQPTSTDSERVFSIAGNFCTKLRSSMKFELLDALIFL 585
[Close]
BLAST of Dimer_Tnp_hAT domain-containing protein vs. Planmine SMEST
Match: SMESG000009273.1 (SMESG000009273.1)

HSP 1 Score: 88.9669 bits (219), Expect = 1.259e-19
Identity = 56/146 (38.36%), Postives = 87/146 (59.59%), Query Frame = 2 
Query:  305 RTTISILFRLSTKNDDILQKYVKTEFG*ELMLILDSKTRWNSLLTILERFLKLKSAIQKALIDIKSSYPFLADKFN--LLSETVATITPIKYALEAICRQDANLLTADATLKLLICTLGKINCVLANDMKNALEFRIKKRRTNLSG 736
            R  + I  R  TKND+ LQKYVK +F   + L  D KTR +SLL +LE+F  +KS I+KAL+D         D++   ++ + V  ++ IK  ++ ICR+DANL+  D  ++ ++  L +    ++N++ +AL  RIK RRT LS 
Sbjct:  111 RKIVHIFKRSQTKNDNYLQKYVKIDFWKVIHLKSDCKTRRSSLLCMLEKFHMVKSCIKKALVDFHIDESLKMDEYEYTIVLDLVNVLSTIKLTVDEICRRDANLIIVDIAIEFMLKKLEQKISNISNEI-SALVIRIKGRRTQLSS 255

HSP 2 Score: 50.0618 bits (118), Expect = 1.556e-6
Identity = 26/61 (42.62%), Postives = 42/61 (68.85%), Query Frame = 1 
Query:  112 VMKEVGKLVNASQHLCFAHGIQLAVLSVLYRNDTSTEV---ELLADLPEN-EHSSDEDSAD 282
            VMK+VGK++  +Q LCFAH +QLA++SVLY+    +E+   E  ++  EN E+  D++ A+
Sbjct:    7 VMKKVGKILVTAQQLCFAHAVQLAIISVLYKPSIHSELSKYENESNFIENLEYEYDDEEAN 67
[Close]
BLAST of Dimer_Tnp_hAT domain-containing protein vs. Planmine SMEST
Match: SMESG000077975.1 (SMESG000077975.1)

HSP 1 Score: 85.5001 bits (210), Expect = 1.704e-19
Identity = 46/133 (34.59%), Postives = 77/133 (57.89%), Query Frame = 2 
Query:  323 LFRLSTKNDDILQKYVKTEFG*ELMLILDSKTRWNSLLTILERFLKLKSAIQKALIDIKSSYPFLADKFNLLSETVATITPIKYALEAICRQDANLLTADATLKLLICTLGKINCVLANDMKNALEFRIKKRR 721
             FR S   +DILQ+YV+ + G +L LILD +TRWNS++ ++ER + L+  I+ AL +I     +    FN+L+  +  + P + A++ + +  + LLTA+  L  L   + + N  L     N+L+ RI +RR
Sbjct:  314 FFRKSPVRNDILQRYVEDKEGKKLKLILDCRTRWNSIIPMMERLVLLQVCIKHALEEIGRDDLYNEKDFNILANLIEILKPTELAVKELSKHTSTLLTAEGVLTFLFTQMKQNNTALGKKFYNSLKIRISERR 446

HSP 2 Score: 29.6462 bits (65), Expect = 1.704e-19
Identity = 25/94 (26.60%), Postives = 44/94 (46.81%), Query Frame = 1 
Query:   52 QKLSIFNISMEKDVVCITID---VMKEVGKLVNASQHLCFAHGIQLAVLSVLYRNDTSTEVELLADLPENEHS-SDEDSADAGLGAGNQNYNFN 321
            QKL  + I + ++++  T D   VMK+  +L       C  H I LAV+   Y  +      ++ ++ ++E++  DED       AG  N N +
Sbjct:  197 QKLMDYGIKL-RNILTSTQDGASVMKKYAQLNKIENQFCINHAIHLAVVETFYNKNN-----VIDEISDSENADGDEDENQFNSFAGYHNLNID 284
[Close]
BLAST of Dimer_Tnp_hAT domain-containing protein vs. Planmine SMEST
Match: SMESG000036917.1 (SMESG000036917.1)

HSP 1 Score: 90.1225 bits (222), Expect = 5.131e-19
Identity = 57/138 (41.30%), Postives = 78/138 (56.52%), Query Frame = 2 
Query:  323 LFRLSTKNDDILQKYVKTEFG*ELMLILDSKTRWNSLLTILERFLKLKSAIQKALIDIKSSYPFLADKFNLLSETVATITPIKYALEAICRQDANLLTADATLKLLICTLGKINCVLANDMKNALEFRIKKRRTNLSG 736
            +FR S   +D  Q+        + +LILD+KTRWNSL+ +++RF +LKS IQK+LIDIKS       KF   S  ++T+ PIK  +EA+CR+DA LLT+   LK                   AL  RI +RRT +SG
Sbjct:  414 IFRNSPTTNDGQQE--------DKVLILDTKTRWNSLVNMIKRFDQLKSYIQKSLIDIKSKITICDHKFEQASTLLSTLHPIKITVEALCRRDAKLLTSKELLK-------------------ALNTRISQRRTEISG 524

HSP 2 Score: 62.3882 bits (150), Expect = 3.832e-10
Identity = 38/103 (36.89%), Postives = 60/103 (58.25%), Query Frame = 1 
Query:    1 F*NLGLACVVG-----RCVDLFQ-KLSIFNISMEKDVVCITID---VMKEVGKLVNASQHLCFAHGIQLAVLSVLYRNDTSTEVELLADLPENEHSSDEDSAD 282
            F +LG+  + G     +C  + + KL  FNI++EK++VC+T D   V+ +VG LV+  Q LCFAHGIQL V+ +LY  D  +   +  +   +E  +D   +D
Sbjct:  263 FCSLGMVRIHGSMHSEKCKLIVKTKLQDFNINLEKNIVCMTADGASVILKVGSLVSNYQLLCFAHGIQLVVIDILYTKDKYSNQRVPKNTQNDEIQADTSDSD 365
The following BLAST results are available for this feature:
BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A4C1V2H51.145e-4141.74Uncharacterized protein OS=Eumeta variegata OX=151... [more]
A0A4C1XQA52.398e-4054.55Uncharacterized protein OS=Eumeta variegata OX=151... [more]
A0A4C1X5B58.222e-4054.55Uncharacterized protein OS=Eumeta variegata OX=151... [more]
A0A4C1X3838.909e-3752.45Uncharacterized protein OS=Eumeta variegata OX=151... [more]
A0A226DDR72.355e-3340.56Putative AC9 transposase OS=Folsomia candida OX=15... [more]
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Dimer_Tnp_hAT domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000061313.14.206e-2335.07SMESG000061313.1[more]
SMESG000022344.11.290e-2032.09SMESG000022344.1[more]
SMESG000009273.11.259e-1938.36SMESG000009273.1[more]
SMESG000077975.11.704e-1934.59SMESG000077975.1[more]
SMESG000036917.15.131e-1941.30SMESG000036917.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30019833 ID=SMED30019833|Name=Dimer_Tnp_hAT domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1269bp
TTTTAGAATTTGGGTCTCGCCTGTGTTGTAGGACGGTGTGTAGACCTTTT
TCAAAAATTAAGCATTTTCAATATTTCGATGGAAAAAGATGTTGTTTGCA
TAACCATAGATGTCATGAAAGAGGTTGGAAAACTAGTTAATGCTTCTCAG
CACCTTTGTTTTGCGCATGGGATACAGTTAGCAGTCTTAAGTGTTTTGTA
CCGTAATGACACATCTACTGAAGTGGAGTTGCTTGCAGATTTGCCGGAAA
ACGAACATTCAAGTGATGAAGATTCAGCTGATGCAGGTTTAGGTGCTGGC
AACCAGAACTACAATTTCAATATTATTTCGGCTTTCTACTAAAAACGATG
ACATCTTGCAAAAGTACGTGAAAACCGAATTTGGATAAGAGCTCATGTTG
ATTTTGGACTCAAAAACACGGTGGAACAGCTTACTAACTATATTGGAGCG
GTTCTTAAAATTGAAGTCAGCTATTCAAAAAGCTTTGATCGATATTAAAT
CAAGCTATCCGTTTTTGGCAGATAAATTCAACCTACTGTCTGAAACTGTT
GCTACCATTACACCAATTAAATACGCCCTTGAAGCTATCTGCCGTCAAGA
CGCTAATCTATTAACTGCAGATGCAACGTTAAAATTACTAATTTGCACAT
TGGGAAAAATAAACTGCGTTCTTGCGAACGACATGAAGAATGCATTAGAA
TTCCGAATTAAGAAAAGAAGAACCAATTTATCAGGTTTGTTGCAGTATTT
GCACAGTTCATCTTTGAATAGTTCGTCTTCCCCCACTGACCTATTCCCTG
CTTTGAAGAAAATCACGATAAAGAGACTCTTATTGAATATGGTTCAACGA
CTATTTATATCAGAATCAAATAATGAAAATGACTAACTACGACAGCAGCA
TAGCGACGAAAATGTTAGTATGAAAGTTTCGATATTAGAAGAAACCGAAA
ACGACTCCGATACGATTAAGCAAAAAATGCTGTACCTGACAAACGGAACA
CATTTTCTTCCACATCTTCAATAATCGAAAAAACTTTTTAAACGTGAGCT
TGCTCAATTTGAAGATATGGAAATCGCAGTAAATGGTTGGATCTTTGTTA
TAACACCTTAAAATAAATTCCTCCTACCAGTGAGAAATCGGAGAGCGCGT
TTTCCGTATCCGGAAACTTTTGTACAAAACTGAGATCACGATTAAGCGAT
GAAACGATTGATAATTTAAAATTTTTCTACGAGCTCATTTTAATAAAATT
TAAAAACATTTTCCCAGAA
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protein sequence of SMED30019833-orf-1

>SMED30019833-orf-1 ID=SMED30019833-orf-1|Name=SMED30019833-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=164bp
MLILDSKTRWNSLLTILERFLKLKSAIQKALIDIKSSYPFLADKFNLLSE
TVATITPIKYALEAICRQDANLLTADATLKLLICTLGKINCVLANDMKNA
LEFRIKKRRTNLSGLLQYLHSSSLNSSSSPTDLFPALKKITIKRLLLNMV
QRLFISESNNEND*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0046983protein dimerization activity
GO:0003676nucleic acid binding
GO:0016301kinase activity
Vocabulary: INTERPRO
TermDefinition
IPR012337RNaseH-like_sf
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
Vocabulary: biological process
TermDefinition
GO:0016310phosphorylation