PiggyBac transposable element-derived protein 4

Overview
NamePiggyBac transposable element-derived protein 4
Smed IDSMED30019820
Length (bp)2338
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of PiggyBac transposable element-derived protein 4 (SMED30019820) t-SNE clustered cells

Violin plots show distribution of expression levels for PiggyBac transposable element-derived protein 4 (SMED30019820) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of PiggyBac transposable element-derived protein 4 (SMED30019820) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for PiggyBac transposable element-derived protein 4 (SMED30019820) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30019820

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
epidermisSMED30019820SMESG000065350.1 SMESG000058681.1 SMESG000023666.1 dd_Smed_v4_9347_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30019820SMESG000065350.1 SMESG000058681.1 SMESG000023666.1 dd_Smed_v4_9347_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30019820SMESG000065350.1 SMESG000058681.1 SMESG000023666.1 dd_Smed_v4_9347_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30019820SMESG000041298.1 Contig13493newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30019820SMESG000041298.1 Contig13493uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
nervous systemSMED30019820 dd_Smed_v4_22567_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30019820 dd_Smed_v4_22567_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30019820 dd_Smed_v4_22567_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Human
Match: PGBD4 (piggyBac transposable element derived 4 [Source:HGNC Symbol;Acc:HGNC:19401])

HSP 1 Score: 173.326 bits (438), Expect = 5.210e-86
Identity = 91/212 (42.92%), Postives = 130/212 (61.32%), Query Frame = 2 
Query:  818 QQQLPFSGTPGINVHISMPGNRLEYFGLFVTDHIVQSIVTETNRRHGLRFANP*-LKARSRL**WSITNPDEIKGFIAIILYQGLVQKPQTAMYWSTNLMLATPYIRRIMTND-------------NKTLSEAETNTEKSFYKIKKIFEAVYSRFSIIYKSGQNIAIDESLMI*KWILAKKQYILLKRARFGLKLYFLCESGSGYIWKEFTY 1411
            +Q+  F+GTPG  V +S   + L+YF LF T+ +V  I  ETN +  L  + P   K  SR+  W  T+ DE+K F A++L QG+VQKP+  M+WST  +L TPY+R+IMT +             N ++S  ++  + S  KIK +F+ + ++FS +Y   +NIA+DESLM+ K  LA KQY+  KR RFGLKLY LCES SGY+W    +
Sbjct:   83 RQRYDFTGTPGRKVDVSDITDPLQYFELFFTEELVSKITRETNAQAALLASKPPGPKGFSRMDKWKDTDNDELKVFFAVMLLQGIVQKPELEMFWSTRPLLDTPYLRQIMTGERFLLLFRCLHFVNNSSISAGQSKAQISLQKIKPVFDFLVNKFSTVYTPNRNIAVDESLMLFKGPLAMKQYLPTKRVRFGLKLYVLCESQSGYVWNALVH 294

HSP 2 Score: 92.8189 bits (229), Expect = 5.210e-86
Identity = 64/191 (33.51%), Postives = 95/191 (49.74%), Query Frame = 3 
Query: 1638 VTRYCGK*MALKCHDKKDVSMMSTFHTAEVQDV---------LAIVVQEKSLWSSAITMPTWVLLMFRTRCLHHTQLKERDSKFGITELFRHLMNKVSLNCYLLFKK-QIENKLIHVQFRLKLIEWLIDVYHMPEHQADTGHTATAGGDPFRLTGCHFPTYGLVTAKKMNAQRICAVCSSK-EVDGKKIRR 2177
            V R+CG+ MALK  D K+V+M+STFH   V +V            V+ + +    A+     +L  + +    H        K    + F HL++   LN Y+LFKK   E+ + H+ FRL LIE +++ +H P  Q   G   +    P RL+G HFP     T+ K N    C +C S+ + DGKKIR+
Sbjct:  375 VARFCGELMALKWCDGKEVTMLSTFHNDTVIEVNNRNGKKTKRPRVIVDYNENMGAVDSADQMLTSYPSERKRH--------KVWYKKFFHHLLHITVLNSYILFKKDNPEHTMSHINFRLALIERMLEKHHKPGQQHLRGRPCSDDVTPLRLSGRHFPKSIPATSGKQNPTGRCKICCSQYDKDGKKIRK 557

HSP 3 Score: 77.0258 bits (188), Expect = 5.210e-86
Identity = 38/67 (56.72%), Postives = 49/67 (73.13%), Query Frame = 1 
Query: 1432 NNLETSKIVITLLQNLPRKVYTVYMDNYYTSPMLFRELISNNTDAVGTVRLNRKNIPADPKK*IVLG 1632
            + L++S+IV+TL+ +L  + Y V++DN+  SPMLFREL  N TDAVGT RLNRK IP D KK I  G
Sbjct:  306 DGLKSSRIVLTLVNDLLGQGYCVFLDNFNISPMLFRELHQNRTDAVGTARLNRKQIPNDLKKRIAKG 372

HSP 4 Score: 39.2762 bits (90), Expect = 5.210e-86
Identity = 14/23 (60.87%), Postives = 17/23 (73.91%), Query Frame = 1 
Query: 2176 ES*YESADCDVRLCAAPCFQIYH 2244
            E+ Y  A+CDV LC  PCF+IYH
Sbjct:  558 ETRYFCAECDVPLCVVPCFEIYH 580

HSP 5 Score: 64.3142 bits (155), Expect = 5.563e-10
Identity = 27/39 (69.23%), Postives = 33/39 (84.62%), Query Frame = 2 
Query: 1757 EKPVVICDYNANMGAVDVSDQMLTSYPAERKRLEVWYNR 1873
            ++P VI DYN NMGAVD +DQMLTSYP+ERKR +VWY +
Sbjct:  416 KRPRVIVDYNENMGAVDSADQMLTSYPSERKRHKVWYKK 454
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Zebrafish
Match: pgbd4 (piggyBac transposable element derived 4 [Source:ZFIN;Acc:ZDB-GENE-091204-417])

HSP 1 Score: 67.781 bits (164), Expect = 1.577e-23
Identity = 56/207 (27.05%), Postives = 94/207 (45.41%), Query Frame = 2 
Query:  842 TPGINVHISMPGNRLEYFGLFVTDHIVQSIVTETNRRHGLRFANP*LKARSRL**WSITNPDEIKGFIAIILYQGLVQKPQTAMYWSTNLMLATPYIRRIMT-------------NDNKTLSEAETNTEKSFY----KIKKIFEAVYSRFSIIYKSGQNIAIDESLMI*KWILAKKQYILLKRARFGLKLYFLCESGSGYIWKEFTY 1411
            TPG  +  +   + L+YF LF +  ++Q IV  +N    +R      +AR R   W      ++K FIA+++Y G+V+      YW  + + + PY  +IM+             +D K  +E +       Y    KIK +++ +       +   QNI+IDE ++  K      QYI  +  ++G KL+ L +S   Y W  F Y
Sbjct:  141 TPGPQLISTASFSVLQYFELFFSQPVMQKIVDHSNIFGAMRH-----EARKRK--WKDITVADLKSFIAMVIYMGIVKCFNLQDYWRMSDVYSLPYPAKIMSRIKFLTISWALHLSDPKVDAENDKKRGTPQYDPLCKIKPLYQDIRDACKASFHPHQNISIDERMVASKARHGLIQYI-SEPTKWGYKLFVLADSLCAYTWDFFIY 339

HSP 2 Score: 46.2098 bits (108), Expect = 1.577e-23
Identity = 19/35 (54.29%), Postives = 26/35 (74.29%), Query Frame = 1 
Query: 1492 YTVYMDNYYTSPMLFRELISNNTDAVGTVRLNRKN 1596
            Y +++DN+YTSP LFR+L+S    A GT+R NR N
Sbjct:  369 YKLFVDNFYTSPTLFRDLLSKKIGACGTIRPNRIN 403

HSP 3 Score: 36.1946 bits (82), Expect = 1.577e-23
Identity = 43/170 (25.29%), Postives = 68/170 (40.00%), Query Frame = 3 
Query: 1680 DKKDVSMMSTFHTAEVQDVLAIVVQEK-SLWS-SAITMPTWVLLMFR-------TRCLHHTQLKERDSKFGITELFRHLMNKVSLNCYLLFKKQIENK----LIHVQFRLKLIEWLIDVYHMPEHQADTGHTATAGGDPFRLTGCHFPTYGLVTAKKMNAQRICAVCSSK 2150
            DK+DV M STFH A   D +   V+ K S+W      +P  VL   +       +  L       R +K      F H ++   +N ++L ++  ++K    +    FR  LI  L  +         +  T+T    P   TG H P Y  ++      +R C +C  K
Sbjct:  429 DKRDVLMCSTFHKAYNGDYIKRNVKGKGSVWGVKEFPVPGAVLDYNKYKGGEDVSNALIGYYTVFRQTKKWYRSFFYHFVDIAIVNAFILHQQMAKSKNQKAMTQKAFRETLITELTGI--------GSASTSTPKA-PVPTTGLHLPRY--ISGDSTVGRRKCKLCQLK 587
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Zebrafish
Match: pgbd4 (piggyBac transposable element derived 4 [Source:ZFIN;Acc:ZDB-GENE-091204-417])

HSP 1 Score: 67.781 bits (164), Expect = 1.542e-21
Identity = 56/207 (27.05%), Postives = 94/207 (45.41%), Query Frame = 2 
Query:  842 TPGINVHISMPGNRLEYFGLFVTDHIVQSIVTETNRRHGLRFANP*LKARSRL**WSITNPDEIKGFIAIILYQGLVQKPQTAMYWSTNLMLATPYIRRIMT-------------NDNKTLSEAETNTEKSFY----KIKKIFEAVYSRFSIIYKSGQNIAIDESLMI*KWILAKKQYILLKRARFGLKLYFLCESGSGYIWKEFTY 1411
            TPG  +  +   + L+YF LF +  ++Q IV  +N    +R      +AR R   W      ++K FIA+++Y G+V+      YW  + + + PY  +IM+             +D K  +E +       Y    KIK +++ +       +   QNI+IDE ++  K      QYI  +  ++G KL+ L +S   Y W  F Y
Sbjct:  141 TPGPQLISTASFSVLQYFELFFSQPVMQKIVDHSNIFGAMRH-----EARKRK--WKDITVADLKSFIAMVIYMGIVKCFNLQDYWRMSDVYSLPYPAKIMSRIKFLTISWALHLSDPKVDAENDKKRGTPQYDPLCKIKPLYQDIRDACKASFHPHQNISIDERMVASKARHGLIQYI-SEPTKWGYKLFVLADSLCAYTWDFFIY 339

HSP 2 Score: 46.2098 bits (108), Expect = 1.542e-21
Identity = 19/35 (54.29%), Postives = 26/35 (74.29%), Query Frame = 1 
Query: 1492 YTVYMDNYYTSPMLFRELISNNTDAVGTVRLNRKN 1596
            Y +++DN+YTSP LFR+L+S    A GT+R NR N
Sbjct:  369 YKLFVDNFYTSPTLFRDLLSKKIGACGTIRPNRIN 403

HSP 3 Score: 29.261 bits (64), Expect = 1.542e-21
Identity = 43/179 (24.02%), Postives = 67/179 (37.43%), Query Frame = 3 
Query: 1680 DKKDVSMMSTFHTAEVQDVLAIVVQEK-SLWS-SAITMPTWVL--------------LMFRTRCLHHTQLKERDSKFGITELFRHLMNKVSLNCYLLFKKQIENK----LIHVQFRLKLIEWL--IDVYHMPEHQADTGHTATAGGDPFRLTGCHFPTYGLVTAKKMNAQRICAVCSSK 2150
            DK+DV M STFH A   D +   V+ K S+W      +P  VL              L+     LH T+   R         F H ++   +N ++L ++  ++K    +    FR  L+  L  I   + P  +A    T             H P Y  +       +R C +C+ K
Sbjct:  429 DKRDVLMCSTFHKAYNGDYIKRNVKGKGSVWGVKEFPVPGAVLDYNKHMGGVDVSDALIGYYTVLHKTKKWYR-------SFFYHFVDIAVVNAFILHQQMAKSKNQKAMTQKVFRETLVAELAGIRAGYPPAREAAIASTDN-----------HLPKY--IKGDSTFGRRKCKLCTHK 587
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Zebrafish
Match: CABZ01069162.1 (pep scaffold:GRCz11:KN149950.1:74:5493:1 gene:ENSDARG00000115161.1 transcript:ENSDART00000191202.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CABZ01069162.1)

HSP 1 Score: 66.6254 bits (161), Expect = 2.535e-18
Identity = 48/198 (24.24%), Postives = 86/198 (43.43%), Query Frame = 2 
Query:  884 LEYFGLFVTDHIVQSIVTETN-----RRHGLRFANP*LKARSRL**WSITNPDEIKGFIAIILYQGLVQKPQTAMYWSTNLMLATPYIRRIMT-----------------NDNKTLSEAETNTEKSFYKIKKIFEAVYSRFSIIYKSGQNIAIDESLMI*KWILAKKQYILLKRARFGLKLYFLCESGSGYIWKEFTY 1411
            L+ F LF T  ++Q+I+  +N     +R G   +            W   + +++K ++A++++ GL++      YW  + + + P   RIM+                 +D        T       KIK +++ +       Y  GQNI+IDE ++  K     KQY+  K  ++G KL+ L +S  GY  + F Y
Sbjct:  222 LQLFQLFFTPSVLQTIMQHSNAYATKQRQGTDKS------------WKNISVEDLKSYLALVIFMGLLKVCALTDYWRRSDVYSVPLPTRIMSCRKFLNIANALHLSDPVDDEANEGRKGTAAYDRLGKIKPLYDNIRDACRAFYHPGQNISIDERMVASKARSGLKQYMKNKPTKWGYKLFVLADSQCGYTSEFFVY 407

HSP 2 Score: 46.2098 bits (108), Expect = 2.535e-18
Identity = 20/37 (54.05%), Postives = 26/37 (70.27%), Query Frame = 1 
Query: 1492 YTVYMDNYYTSPMLFRELISNNTDAVGTVRLNRKNIP 1602
            Y +Y+DN+YTSP LFR+L+S    A GT+R NR   P
Sbjct:  436 YKLYVDNFYTSPKLFRDLLSKRIYACGTIRPNRIGFP 472
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Zebrafish
Match: si:ch211-130m23.5 (si:ch211-130m23.5 [Source:ZFIN;Acc:ZDB-GENE-131121-298])

HSP 1 Score: 80.1073 bits (196), Expect = 4.729e-18
Identity = 61/250 (24.40%), Postives = 108/250 (43.20%), Query Frame = 2 
Query:  728 VDYESHATVDYYHDVVDEETFHLN--NAPQQIQQQLPFSGTPGINVHISMPGNRLEYFGLFVTDHIVQSIVTETN-------RRHGLRFANP*LKARSRL**WSITNPDEIKGFIAIILYQGLVQKPQTAMYWSTNLMLATPYIRRIMT-------NDNKTLSEAETNTEKS------FYKIKKIFEAVYSRFSIIYKSGQNIAIDESLMI*KWILAKKQYILLKRARFGLKLYFLCESGSGYIWKEFTY 1411
            ++ E H     Y DV+D+E    +    P Q+    PF+   G    +    + L+YF L   D + + +V +TN       RR G   A+           W  T+  E++ ++ + +  G+ Q P   MYW++++ +     ++ MT       N +  L + E+   K        YKI+ + + V +     Y   + + IDE  +  K   +  QY+  K  R GLK++ +C+S SGY  +   Y
Sbjct:  141 LEEEEHPLFSTY-DVLDDECTSPDFWGEPDQVISIEPFTADSGPQHSLGDDADTLDYFRLLFPDSLFKHMVEQTNNYALYRQRRSGRSDAH-----------WHPTDIREMRAYVGLNILMGINQLPDYGMYWASDIFIGNAGFKKTMTARRFEKLNQHLHLCDRESEPSKGELGYDGLYKIRPLLDMVGNTMWDAYLPNRCLTIDECSVASKGRFSPTQYMPSKPFRKGLKVWMMCDSRSGYCHRTRIY 378

HSP 2 Score: 31.5722 bits (70), Expect = 4.729e-18
Identity = 18/63 (28.57%), Postives = 35/63 (55.56%), Query Frame = 1 
Query: 1450 KIVITLLQNLPRKVYTVYMDNYYTSPMLFRELISNNTDAVGTVRLNRKNIPADPKK*IVLGKL 1638
            K+V +L++ L  + + +++D+++TS  L + L+     A G    +R+  P D  K   +GKL
Sbjct:  394 KVVSSLVRGLEGRHHHIFIDSFFTSVPLLQHLLHQGFYACGPTNPSRQGYP-DALKPRNVGKL 455
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Zebrafish
Match: si:dkey-19f4.2 (si:dkey-19f4.2 [Source:ZFIN;Acc:ZDB-GENE-121214-262])

HSP 1 Score: 56.6102 bits (135), Expect = 1.844e-14
Identity = 57/234 (24.36%), Postives = 102/234 (43.59%), Query Frame = 2 
Query:  800 NAPQ--QIQQQLPF---SGTPGINVHISMPGNRLEYFGLFVTDHIVQSIVTETNRRHGLRFANP*LKARSRL**WSITNPDEIKGFIAIILYQGLVQKPQTAMYWSTNLMLATPYIRRIMTND-----NKT--LSEAETNTEKS----------FYKIKKIFEAVYSRFSIIYKSGQNIAIDESLMI*KWILAKKQYILLKRARFGLKLYFLCESGSGYIWKEFTYRTSNAFVT 1435
            N P+     QQLP       PG+   + +  +  + F  F +   V+ I   TN       A   L    +L  W     D    +I +++Y  L++ P+   YW  +  L+  +   +MT D     ++T  LS  + + E             Y++K ++  +      +++  + IAIDE ++  K  +A KQY+  K  ++G+KL+ L +S +GY   +FT  T  +  T
Sbjct:   95 NGPEVADTAQQLPKFCPKRAPGLQGFVGVTYSLAQIFRQFFSVTTVRVICCNTNIN-----AEKELSKGKKLA-WEKLTSDTFLKYIGLLIYMALLKLPRVRDYWRRDSHLSVHFPVSVMTRDRFMAISRTVHLSNPDDDIENDRKKGTPDYDPLYRLKPLYITIKDTCKSMWQPRKQIAIDERMVATKAKIALKQYMKAKPIKWGVKLFVLADSSNGYT-SDFTIYTGKSRFT 321

HSP 2 Score: 43.1282 bits (100), Expect = 1.844e-14
Identity = 18/36 (50.00%), Postives = 27/36 (75.00%), Query Frame = 1 
Query: 1492 YTVYMDNYYTSPMLFRELISNNTDAVGTVRLNRKNI 1599
            Y +Y+DN+YTSP LFR+L ++   A GT + +RKN+
Sbjct:  344 YHLYVDNFYTSPKLFRDLYASGFVACGTFKDSRKNV 379
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Xenopus
Match: ENSXETT00000026903.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:4:20337945:20339225:1 gene:ENSXETG00000013290.1 transcript:ENSXETT00000026903.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 120.939 bits (302), Expect = 7.362e-35
Identity = 69/200 (34.50%), Postives = 107/200 (53.50%), Query Frame = 2 
Query:  830 PFSGTPGINVHISMPGNRLEYFGLFVTDHIVQSIVTETNRRHGLRFANP*LKARSRL**WSITNPDEIKGFIAIILYQGLVQKPQTAMYWSTNLMLATPYIRRIMT-------------NDNKTLSEAETNTEKSFYKIKKIFEAVYSRFSIIYKSGQNIAIDESLMI*KWILAKKQYILLKRARFGLKLYFLCESGSGY 1390
            PF+  PG++V  +     +++F L++T+ I+Q +V  TN       A+  L   SR   W  TN +EIK F+A+ L  GLV++   A YW TN +L+ P    +M              NDN T            YK++ + +++  RF+ +Y   QN+ +DESL++ K  L  +QYI  KR+R+G+K Y LCES +GY
Sbjct:  104 PFTAVPGLSVDTTN-FEIIDFFNLYITEAILQDMVRYTNLYAEQYLASNPLPGFSRAQAWYPTNINEIKRFLALTLAMGLVERNSLASYWDTNTVLSIPLFSAVMPRNRYQILLRFLHFNDNTTAVAPNEPGYDRLYKLRPLIDSLSQRFAEVYTPSQNVCVDESLLLFKGRLKFRQYIPSKRSRYGMKFYKLCESSTGY 302

HSP 2 Score: 48.1358 bits (113), Expect = 7.362e-35
Identity = 27/62 (43.55%), Postives = 37/62 (59.68%), Query Frame = 1 
Query: 1423 CICNNLETSKIVITLLQNLPRKVYTVYMDNYYTSPMLFRELISNNTDAVGTVRLNRKNIPAD 1608
            C  +   + KIV  L+  L  + Y +Y+DN+YTS  LFR L S +T A GTV  NRK +P +
Sbjct:  321 CPTDLTTSGKIVWELITPLLGRGYHLYVDNFYTSIPLFRALNSLDTPACGTVNRNRKGLPRE 382
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Xenopus
Match: ENSXETT00000040662.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:2:103014086:103015508:-1 gene:ENSXETG00000019536.1 transcript:ENSXETT00000040662.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 120.939 bits (302), Expect = 7.473e-35
Identity = 69/200 (34.50%), Postives = 107/200 (53.50%), Query Frame = 2 
Query:  830 PFSGTPGINVHISMPGNRLEYFGLFVTDHIVQSIVTETNRRHGLRFANP*LKARSRL**WSITNPDEIKGFIAIILYQGLVQKPQTAMYWSTNLMLATPYIRRIMT-------------NDNKTLSEAETNTEKSFYKIKKIFEAVYSRFSIIYKSGQNIAIDESLMI*KWILAKKQYILLKRARFGLKLYFLCESGSGY 1390
            PF+  PG++V  +     +++F L++T+ I+Q +V  TN       A+  L   SR   W  TN +EIK F+A+ L  GLV++   A YW TN +L+ P    +M              NDN T            YK++ + +++  RF+ +Y   QN+ +DESL++ K  L  +QYI  KR+R+G+K Y LCES +GY
Sbjct:  104 PFTAVPGLSVDTTN-FEIIDFFNLYITEAILQDMVRYTNLYAEQYLASNPLPGFSRAQAWYPTNINEIKRFLALTLAMGLVERNSLASYWDTNTVLSIPLFSAVMPRNRYQILLRFLHFNDNTTAVAPNEPGYDRLYKLRPLIDSLSQRFAEVYTPSQNVCVDESLLLFKGRLKFRQYIPSKRSRYGMKFYKLCESSTGY 302

HSP 2 Score: 48.1358 bits (113), Expect = 7.473e-35
Identity = 27/62 (43.55%), Postives = 37/62 (59.68%), Query Frame = 1 
Query: 1423 CICNNLETSKIVITLLQNLPRKVYTVYMDNYYTSPMLFRELISNNTDAVGTVRLNRKNIPAD 1608
            C  +   + KIV  L+  L  + Y +Y+DN+YTS  LFR L S +T A GTV  NRK +P +
Sbjct:  321 CPTDLTTSGKIVWELITPLLGRGYHLYVDNFYTSIPLFRALNSLDTPACGTVNRNRKGLPRE 382
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Xenopus
Match: ENSXETT00000029105.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:3:71954519:71956550:-1 gene:ENSXETG00000004868.1 transcript:ENSXETT00000029105.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 120.553 bits (301), Expect = 8.598e-35
Identity = 69/200 (34.50%), Postives = 107/200 (53.50%), Query Frame = 2 
Query:  830 PFSGTPGINVHISMPGNRLEYFGLFVTDHIVQSIVTETNRRHGLRFANP*LKARSRL**WSITNPDEIKGFIAIILYQGLVQKPQTAMYWSTNLMLATPYIRRIMT-------------NDNKTLSEAETNTEKSFYKIKKIFEAVYSRFSIIYKSGQNIAIDESLMI*KWILAKKQYILLKRARFGLKLYFLCESGSGY 1390
            PF+  PG++V  +     +++F L++T+ I+Q +V  TN       A+  L   SR   W  TN +EIK F+A+ L  GLV++   A YW TN +L+ P    +M              NDN T            YK++ + +++  RF+ +Y   QN+ +DESL++ K  L  +QYI  KR+R+G+K Y LCES +GY
Sbjct:  104 PFTAVPGLSVDTTN-FEIIDFFNLYITEAILQDMVRYTNLYAEQYLASNPLPGFSRAQAWYPTNINEIKRFLALTLAMGLVERNSLASYWDTNTVLSIPLFSAVMPRNRYQILLRFLHFNDNTTAVAPNEPGYDRLYKLRPLIDSLSQRFAEVYTPSQNVCVDESLLLFKGRLKFRQYIPSKRSRYGMKFYKLCESSTGY 302

HSP 2 Score: 48.1358 bits (113), Expect = 8.598e-35
Identity = 27/62 (43.55%), Postives = 37/62 (59.68%), Query Frame = 1 
Query: 1423 CICNNLETSKIVITLLQNLPRKVYTVYMDNYYTSPMLFRELISNNTDAVGTVRLNRKNIPAD 1608
            C  +   + KIV  L+  L  + Y +Y+DN+YTS  LFR L S +T A GTV  NRK +P +
Sbjct:  321 CPTDLTTSGKIVWELITPLLGRGYHLYVDNFYTSIPLFRALNSLDTPACGTVNRNRKGLPRE 382
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Xenopus
Match: epc1 (enhancer of polycomb homolog 1 [Source:Xenbase;Acc:XB-GENE-493119])

HSP 1 Score: 120.553 bits (301), Expect = 1.111e-34
Identity = 69/200 (34.50%), Postives = 107/200 (53.50%), Query Frame = 2 
Query:  830 PFSGTPGINVHISMPGNRLEYFGLFVTDHIVQSIVTETNRRHGLRFANP*LKARSRL**WSITNPDEIKGFIAIILYQGLVQKPQTAMYWSTNLMLATPYIRRIMT-------------NDNKTLSEAETNTEKSFYKIKKIFEAVYSRFSIIYKSGQNIAIDESLMI*KWILAKKQYILLKRARFGLKLYFLCESGSGY 1390
            PF+  PG++V  +     +++F L++T+ I+Q +V  TN       A+  L   SR   W  TN +EIK F+A+ L  GLV++   A YW TN +L+ P    +M              NDN T            YK++ + +++  RF+ +Y   QN+ +DESL++ K  L  +QYI  KR+R+G+K Y LCES +GY
Sbjct:  104 PFTAVPGLSVDTTN-FEIIDFFNLYITEAILQDMVRYTNLYAEQYLASNPLPGFSRAQAWYPTNINEIKRFLALTLAMGLVERNSLASYWDTNTVLSIPLFSAVMARNRYQILLRFLHFNDNTTAVAPNEPGYDRLYKLRPLIDSLSQRFAEVYTPSQNVCVDESLLLFKGRLKFRQYIPSKRSRYGIKFYKLCESSTGY 302

HSP 2 Score: 47.7506 bits (112), Expect = 1.111e-34
Identity = 26/62 (41.94%), Postives = 36/62 (58.06%), Query Frame = 1 
Query: 1423 CICNNLETSKIVITLLQNLPRKVYTVYMDNYYTSPMLFRELISNNTDAVGTVRLNRKNIPAD 1608
            C  +   + KIV  L+  L  + Y +Y+DN+YT   LFR L S +T A GTV  NRK +P +
Sbjct:  321 CPTDLTTSGKIVWELITPLLGRGYHLYVDNFYTGIPLFRALNSLDTPACGTVHRNRKGLPRE 382
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Xenopus
Match: ENSXETT00000042643.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:6:70311429:70312834:1 gene:ENSXETG00000002315.1 transcript:ENSXETT00000042643.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 120.553 bits (301), Expect = 1.111e-34
Identity = 69/200 (34.50%), Postives = 107/200 (53.50%), Query Frame = 2 
Query:  830 PFSGTPGINVHISMPGNRLEYFGLFVTDHIVQSIVTETNRRHGLRFANP*LKARSRL**WSITNPDEIKGFIAIILYQGLVQKPQTAMYWSTNLMLATPYIRRIMT-------------NDNKTLSEAETNTEKSFYKIKKIFEAVYSRFSIIYKSGQNIAIDESLMI*KWILAKKQYILLKRARFGLKLYFLCESGSGY 1390
            PF+  PG++V  +     +++F L++T+ I+Q +V  TN       A+  L   SR   W  TN +EIK F+A+ L  GLV++   A YW TN +L+ P    +M              NDN T            YK++ + +++  RF+ +Y   QN+ +DESL++ K  L  +QYI  KR+R+G+K Y LCES +GY
Sbjct:  104 PFTAVPGLSVDTTN-FEIIDFFNLYITEAILQDMVRYTNLYAEQYLASNPLPGFSRAQAWYPTNINEIKRFLALTLAMGLVERNSLASYWDTNTVLSIPLFSAVMARNRYQILLRFLHFNDNTTAVAPNEPGYDRLYKLRPLIDSLSQRFAEVYTPSQNVCVDESLLLFKGRLKFRQYIPSKRSRYGIKFYKLCESSTGY 302

HSP 2 Score: 47.7506 bits (112), Expect = 1.111e-34
Identity = 26/62 (41.94%), Postives = 36/62 (58.06%), Query Frame = 1 
Query: 1423 CICNNLETSKIVITLLQNLPRKVYTVYMDNYYTSPMLFRELISNNTDAVGTVRLNRKNIPAD 1608
            C  +   + KIV  L+  L  + Y +Y+DN+YT   LFR L S +T A GTV  NRK +P +
Sbjct:  321 CPTDLTTSGKIVWELITPLLGRGYHLYVDNFYTGIPLFRALNSLDTPACGTVHRNRKGLPRE 382
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. UniProt/SwissProt
Match: sp|Q96DM1|PGBD4_HUMAN (PiggyBac transposable element-derived protein 4 OS=Homo sapiens OX=9606 GN=PGBD4 PE=2 SV=3)

HSP 1 Score: 173.326 bits (438), Expect = 2.296e-85
Identity = 91/212 (42.92%), Postives = 130/212 (61.32%), Query Frame = 2 
Query:  818 QQQLPFSGTPGINVHISMPGNRLEYFGLFVTDHIVQSIVTETNRRHGLRFANP*-LKARSRL**WSITNPDEIKGFIAIILYQGLVQKPQTAMYWSTNLMLATPYIRRIMTND-------------NKTLSEAETNTEKSFYKIKKIFEAVYSRFSIIYKSGQNIAIDESLMI*KWILAKKQYILLKRARFGLKLYFLCESGSGYIWKEFTY 1411
            +Q+  F+GTPG  V +S   + L+YF LF T+ +V  I  ETN +  L  + P   K  SR+  W  T+ DE+K F A++L QG+VQKP+  M+WST  +L TPY+R+IMT +             N ++S  ++  + S  KIK +F+ + ++FS +Y   +NIA+DESLM+ K  LA KQY+  KR RFGLKLY LCES SGY+W    +
Sbjct:   83 RQRYDFTGTPGRKVDVSDITDPLQYFELFFTEELVSKITRETNAQAALLASKPPGPKGFSRMDKWKDTDNDELKVFFAVMLLQGIVQKPELEMFWSTRPLLDTPYLRQIMTGERFLLLFRCLHFVNNSSISAGQSKAQISLQKIKPVFDFLVNKFSTVYTPNRNIAVDESLMLFKGPLAMKQYLPTKRVRFGLKLYVLCESQSGYVWNALVH 294

HSP 2 Score: 92.8189 bits (229), Expect = 2.296e-85
Identity = 64/191 (33.51%), Postives = 95/191 (49.74%), Query Frame = 3 
Query: 1638 VTRYCGK*MALKCHDKKDVSMMSTFHTAEVQDV---------LAIVVQEKSLWSSAITMPTWVLLMFRTRCLHHTQLKERDSKFGITELFRHLMNKVSLNCYLLFKK-QIENKLIHVQFRLKLIEWLIDVYHMPEHQADTGHTATAGGDPFRLTGCHFPTYGLVTAKKMNAQRICAVCSSK-EVDGKKIRR 2177
            V R+CG+ MALK  D K+V+M+STFH   V +V            V+ + +    A+     +L  + +    H        K    + F HL++   LN Y+LFKK   E+ + H+ FRL LIE +++ +H P  Q   G   +    P RL+G HFP     T+ K N    C +C S+ + DGKKIR+
Sbjct:  375 VARFCGELMALKWCDGKEVTMLSTFHNDTVIEVNNRNGKKTKRPRVIVDYNENMGAVDSADQMLTSYPSERKRH--------KVWYKKFFHHLLHITVLNSYILFKKDNPEHTMSHINFRLALIERMLEKHHKPGQQHLRGRPCSDDVTPLRLSGRHFPKSIPATSGKQNPTGRCKICCSQYDKDGKKIRK 557

HSP 3 Score: 77.0258 bits (188), Expect = 2.296e-85
Identity = 38/67 (56.72%), Postives = 49/67 (73.13%), Query Frame = 1 
Query: 1432 NNLETSKIVITLLQNLPRKVYTVYMDNYYTSPMLFRELISNNTDAVGTVRLNRKNIPADPKK*IVLG 1632
            + L++S+IV+TL+ +L  + Y V++DN+  SPMLFREL  N TDAVGT RLNRK IP D KK I  G
Sbjct:  306 DGLKSSRIVLTLVNDLLGQGYCVFLDNFNISPMLFRELHQNRTDAVGTARLNRKQIPNDLKKRIAKG 372

HSP 4 Score: 39.2762 bits (90), Expect = 2.296e-85
Identity = 14/23 (60.87%), Postives = 17/23 (73.91%), Query Frame = 1 
Query: 2176 ES*YESADCDVRLCAAPCFQIYH 2244
            E+ Y  A+CDV LC  PCF+IYH
Sbjct:  558 ETRYFCAECDVPLCVVPCFEIYH 580

HSP 5 Score: 64.3142 bits (155), Expect = 2.672e-9
Identity = 27/39 (69.23%), Postives = 33/39 (84.62%), Query Frame = 2 
Query: 1757 EKPVVICDYNANMGAVDVSDQMLTSYPAERKRLEVWYNR 1873
            ++P VI DYN NMGAVD +DQMLTSYP+ERKR +VWY +
Sbjct:  416 KRPRVIVDYNENMGAVDSADQMLTSYPSERKRHKVWYKK 454
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. TrEMBL
Match: G3QWT8 (PiggyBac transposable element derived 4 OS=Gorilla gorilla gorilla OX=9595 PE=4 SV=1)

HSP 1 Score: 173.711 bits (439), Expect = 3.623e-83
Identity = 91/212 (42.92%), Postives = 130/212 (61.32%), Query Frame = 2 
Query:  818 QQQLPFSGTPGINVHISMPGNRLEYFGLFVTDHIVQSIVTETNRRHGLRFANP*-LKARSRL**WSITNPDEIKGFIAIILYQGLVQKPQTAMYWSTNLMLATPYIRRIMTND-------------NKTLSEAETNTEKSFYKIKKIFEAVYSRFSIIYKSGQNIAIDESLMI*KWILAKKQYILLKRARFGLKLYFLCESGSGYIWKEFTY 1411
            +Q+  F+GTPG  V +S   + L+YF LF T+ +V  I  ETN +  L  + P   K  SR+  W  T+ DE+K F A++L QG+VQKP+  M+WST  +L TPY+R+IMT +             N ++S  ++  + S  KIK +F+ + ++FS +Y   +NIA+DESLM+ K  LA KQY+  KR RFGLKLY LCES SGY+W    +
Sbjct:   83 RQRYDFTGTPGRKVDVSDITDPLQYFELFFTEELVSKITRETNAQAALLASKPPGPKGFSRMDKWKDTDNDELKVFFAVMLLQGIVQKPELEMFWSTRPLLDTPYLRQIMTGERFLLLFRCLHFVNNSSISAGQSKAQISLQKIKPVFDFLVNKFSTVYTPNRNIAVDESLMLFKGPLAMKQYLPTKRVRFGLKLYVLCESQSGYVWNALVH 294

HSP 2 Score: 93.2041 bits (230), Expect = 3.623e-83
Identity = 64/191 (33.51%), Postives = 95/191 (49.74%), Query Frame = 3 
Query: 1638 VTRYCGK*MALKCHDKKDVSMMSTFHTAEVQDV---------LAIVVQEKSLWSSAITMPTWVLLMFRTRCLHHTQLKERDSKFGITELFRHLMNKVSLNCYLLFKK-QIENKLIHVQFRLKLIEWLIDVYHMPEHQADTGHTATAGGDPFRLTGCHFPTYGLVTAKKMNAQRICAVCSSK-EVDGKKIRR 2177
            V R+CG+ MALK  D K+V+M+STFH   V +V            V+ + +    A+     +L  + +    H        K    + F HL++   LN Y+LFKK   E+ + H+ FRL LIE +++ +H P  Q   G   +    P RL+G HFP     T+ K N    C +C S+ + DGKKIR+
Sbjct:  375 VARFCGELMALKWCDGKEVTMLSTFHNDTVIEVNNRNGKKTKRPRVIVDYNENMGAVDSADQMLTSYPSERKRH--------KVWYKKFFHHLLHITVLNSYILFKKDNPEHTMSHINFRLALIERMLEKHHKPGQQHLRGRPCSDDVTPLRLSGRHFPKSIPATSGKQNPTGRCKICCSQYDKDGKKIRK 557

HSP 3 Score: 77.0258 bits (188), Expect = 3.623e-83
Identity = 38/67 (56.72%), Postives = 49/67 (73.13%), Query Frame = 1 
Query: 1432 NNLETSKIVITLLQNLPRKVYTVYMDNYYTSPMLFRELISNNTDAVGTVRLNRKNIPADPKK*IVLG 1632
            + L++S+IV+TL+ +L  + Y V++DN+  SPMLFREL  N TDAVGT RLNRK IP D KK I  G
Sbjct:  306 DGLKSSRIVLTLVNDLLGQGYCVFLDNFNISPMLFRELHQNRTDAVGTARLNRKQIPNDLKKRIAKG 372

HSP 4 Score: 39.2762 bits (90), Expect = 3.623e-83
Identity = 14/23 (60.87%), Postives = 17/23 (73.91%), Query Frame = 1 
Query: 2176 ES*YESADCDVRLCAAPCFQIYH 2244
            E+ Y  A+CDV LC  PCF+IYH
Sbjct:  558 ETRYFCAECDVPLCVVPCFEIYH 580

HSP 5 Score: 64.3142 bits (155), Expect = 7.783e-7
Identity = 27/39 (69.23%), Postives = 33/39 (84.62%), Query Frame = 2 
Query: 1757 EKPVVICDYNANMGAVDVSDQMLTSYPAERKRLEVWYNR 1873
            ++P VI DYN NMGAVD +DQMLTSYP+ERKR +VWY +
Sbjct:  416 KRPRVIVDYNENMGAVDSADQMLTSYPSERKRHKVWYKK 454
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. TrEMBL
Match: A0A2K6S7R1 (PiggyBac transposable element derived 4 OS=Saimiri boliviensis boliviensis OX=39432 GN=PGBD4 PE=4 SV=1)

HSP 1 Score: 173.711 bits (439), Expect = 4.133e-83
Identity = 92/212 (43.40%), Postives = 130/212 (61.32%), Query Frame = 2 
Query:  818 QQQLPFSGTPGINVHISMPGNRLEYFGLFVTDHIVQSIVTETNRRHGLRFANP*-LKARSRL**WSITNPDEIKGFIAIILYQGLVQKPQTAMYWSTNLMLATPYIRRIMTND-------------NKTLSEAETNTEKSFYKIKKIFEAVYSRFSIIYKSGQNIAIDESLMI*KWILAKKQYILLKRARFGLKLYFLCESGSGYIWKEFTY 1411
            +Q+  F+GTPG  V +S   + L+YF LF T+ +V  I  ETN +  L  + P   K  SR+  W  T+ DE+K F A++L QG+VQKP+  M+WST  +L TPY+R+IMT +             N ++S  ++  + S  KIK +F+ + ++FS +Y   +NIA+DESLM+ K  LA KQYI  KR RFGLKLY LCES SGY+W    +
Sbjct:   83 RQRYDFTGTPGRKVDVSDITDPLQYFELFFTEELVSKITRETNAQAALLASKPPGPKGFSRMDKWKDTDNDELKVFFAVMLLQGIVQKPELEMFWSTRPLLDTPYLRQIMTGERFLLLFRCLHFVNNSSISAGQSKAQISLQKIKPVFDFLVNKFSTVYTPNRNIAVDESLMLFKGPLAIKQYIPTKRVRFGLKLYVLCESQSGYVWNALVH 294

HSP 2 Score: 92.8189 bits (229), Expect = 4.133e-83
Identity = 65/191 (34.03%), Postives = 95/191 (49.74%), Query Frame = 3 
Query: 1638 VTRYCGK*MALKCHDKKDVSMMSTFHTAEVQDV---------LAIVVQEKSLWSSAITMPTWVLLMFRTRCLHHTQLKERDSKFGITELFRHLMNKVSLNCYLLFKK-QIENKLIHVQFRLKLIEWLIDVYHMPEHQADTGHTATAGGDPFRLTGCHFPTYGLVTAKKMNAQRICAVCSSK-EVDGKKIRR 2177
            V R+CG+ MALK  D K+V+M+STFH+  V +V            V+ + +    A+     +L  + +    H        K    + F HL++   LN Y+LFKK   E  + H+ FRL LIE L++ +H P  Q   G   +    P RL+G HFP     T+ K N    C +C S+ + DGKKIR+
Sbjct:  375 VARFCGELMALKWCDTKEVTMLSTFHSDTVIEVNNRNGKKTKKPRVIVDYNENMGAVDSADQMLTSYPSERKRH--------KVWYKKFFHHLLHITVLNSYILFKKDNPEYTMSHINFRLTLIERLLEKHHKPGQQHLRGRPCSDDVTPLRLSGRHFPKSIPPTSGKQNPTGRCKICCSQYDKDGKKIRK 557

HSP 3 Score: 77.0258 bits (188), Expect = 4.133e-83
Identity = 38/67 (56.72%), Postives = 49/67 (73.13%), Query Frame = 1 
Query: 1432 NNLETSKIVITLLQNLPRKVYTVYMDNYYTSPMLFRELISNNTDAVGTVRLNRKNIPADPKK*IVLG 1632
            + L++S+IV+TL+ +L  + Y V++DN+  SPMLFREL  N TDAVGT RLNRK IP D KK I  G
Sbjct:  306 DGLKSSRIVLTLVNDLLGQGYCVFLDNFNISPMLFRELHQNRTDAVGTARLNRKQIPNDLKKRIAKG 372

HSP 4 Score: 39.2762 bits (90), Expect = 4.133e-83
Identity = 14/28 (50.00%), Postives = 19/28 (67.86%), Query Frame = 1 
Query: 2176 ES*YESADCDVRLCAAPCFQIYHIIRKF 2259
            E+ Y  A+CDV LC  PCF+IYH  + +
Sbjct:  558 ETRYFCAECDVPLCVVPCFEIYHTQKNY 585

HSP 5 Score: 65.0846 bits (157), Expect = 4.217e-7
Identity = 28/39 (71.79%), Postives = 33/39 (84.62%), Query Frame = 2 
Query: 1757 EKPVVICDYNANMGAVDVSDQMLTSYPAERKRLEVWYNR 1873
            +KP VI DYN NMGAVD +DQMLTSYP+ERKR +VWY +
Sbjct:  416 KKPRVIVDYNENMGAVDSADQMLTSYPSERKRHKVWYKK 454
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. TrEMBL
Match: A0A2K6JV20 (PiggyBac transposable element derived 4 OS=Rhinopithecus bieti OX=61621 GN=PGBD4 PE=4 SV=1)

HSP 1 Score: 172.94 bits (437), Expect = 6.500e-83
Identity = 91/212 (42.92%), Postives = 129/212 (60.85%), Query Frame = 2 
Query:  818 QQQLPFSGTPGINVHISMPGNRLEYFGLFVTDHIVQSIVTETNRRHGLRFANP*-LKARSRL**WSITNPDEIKGFIAIILYQGLVQKPQTAMYWSTNLMLATPYIRRIMTND-------------NKTLSEAETNTEKSFYKIKKIFEAVYSRFSIIYKSGQNIAIDESLMI*KWILAKKQYILLKRARFGLKLYFLCESGSGYIWKEFTY 1411
            +Q+  F+GTPG  V +S   + L+YF LF T+ +V  I  ETN +  L  + P   K  SR+  W  T+ DE+K F A++L QG+VQKP+  M+WST  +L TPY+R+IMT +             N ++S  ++    S  KIK +F+ + ++FS +Y   +NIA+DESLM+ K  LA KQY+  KR RFGLKLY LCES SGY+W    +
Sbjct:   83 RQRYDFTGTPGRKVDVSDITDPLQYFELFFTEELVSKITRETNAQAALLASKPPGPKGFSRMDKWKDTDNDELKVFFAVMLLQGIVQKPELEMFWSTRPLLDTPYLRQIMTGERFLLLFRCLHFVNNSSISAGQSKAHISLQKIKPVFDFLVNKFSTVYTPNRNIAVDESLMLFKGPLAMKQYVPTKRVRFGLKLYVLCESQSGYVWNALVH 294

HSP 2 Score: 92.0485 bits (227), Expect = 6.500e-83
Identity = 64/191 (33.51%), Postives = 95/191 (49.74%), Query Frame = 3 
Query: 1638 VTRYCGK*MALKCHDKKDVSMMSTFHTAEVQDV---------LAIVVQEKSLWSSAITMPTWVLLMFRTRCLHHTQLKERDSKFGITELFRHLMNKVSLNCYLLFKK-QIENKLIHVQFRLKLIEWLIDVYHMPEHQADTGHTATAGGDPFRLTGCHFPTYGLVTAKKMNAQRICAVCSSK-EVDGKKIRR 2177
            V R+CG+ MALK  D K+V+M+STFH   V +V            V+ + +    A+     +L  + +    H        K    + F HL++   LN Y+LFKK   E+ + H+ FRL LIE +++ +H P  Q   G   +    P RL+G HFP     T+ K N    C +C S+ + DGKKIR+
Sbjct:  375 VARFCGELMALKWCDGKEVTMLSTFHNDTVIEVNNRNGKKTKKPRVIVDYNENMGAVDSADQMLTSYPSERKRH--------KVWYKKFFHHLLHITVLNSYILFKKDNPEHAMSHINFRLALIERMLEKHHKPGQQHLRGRPCSDDVTPLRLSGRHFPKSIPPTSGKQNPTGRCKICCSQYDKDGKKIRK 557

HSP 3 Score: 77.0258 bits (188), Expect = 6.500e-83
Identity = 38/67 (56.72%), Postives = 49/67 (73.13%), Query Frame = 1 
Query: 1432 NNLETSKIVITLLQNLPRKVYTVYMDNYYTSPMLFRELISNNTDAVGTVRLNRKNIPADPKK*IVLG 1632
            + L++S+IV+TL+ +L  + Y V++DN+  SPMLFREL  N TDAVGT RLNRK IP D KK I  G
Sbjct:  306 DGLKSSRIVLTLVNDLLGQGYCVFLDNFNISPMLFRELHQNRTDAVGTARLNRKQIPNDLKKRIAKG 372

HSP 4 Score: 39.6614 bits (91), Expect = 6.500e-83
Identity = 14/28 (50.00%), Postives = 19/28 (67.86%), Query Frame = 1 
Query: 2176 ES*YESADCDVRLCAAPCFQIYHIIRKF 2259
            E+ Y  A+CDV LC  PCF+IYH  + +
Sbjct:  558 ETRYFCAECDVPLCVVPCFEIYHTKKHY 585

HSP 5 Score: 65.4698 bits (158), Expect = 4.005e-7
Identity = 28/39 (71.79%), Postives = 33/39 (84.62%), Query Frame = 2 
Query: 1757 EKPVVICDYNANMGAVDVSDQMLTSYPAERKRLEVWYNR 1873
            +KP VI DYN NMGAVD +DQMLTSYP+ERKR +VWY +
Sbjct:  416 KKPRVIVDYNENMGAVDSADQMLTSYPSERKRHKVWYKK 454
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. TrEMBL
Match: F7FDY8 (PiggyBac transposable element derived 4 OS=Callithrix jacchus OX=9483 GN=PGBD4 PE=4 SV=2)

HSP 1 Score: 173.326 bits (438), Expect = 7.664e-83
Identity = 91/212 (42.92%), Postives = 130/212 (61.32%), Query Frame = 2 
Query:  818 QQQLPFSGTPGINVHISMPGNRLEYFGLFVTDHIVQSIVTETNRRHGLRFANP*-LKARSRL**WSITNPDEIKGFIAIILYQGLVQKPQTAMYWSTNLMLATPYIRRIMTND-------------NKTLSEAETNTEKSFYKIKKIFEAVYSRFSIIYKSGQNIAIDESLMI*KWILAKKQYILLKRARFGLKLYFLCESGSGYIWKEFTY 1411
            +Q+  F+GTPG  V +S   + L+YF LF T+ +V  I  ETN +  L  + P   K  SR+  W  T+ DE+K F A++L QG+VQKP+  M+WST  +L TPY+R+IMT +             N ++S  ++  + S  KIK +F+ + ++FS +Y   +NIA+DESLM+ K  LA KQY+  KR RFGLKLY LCES SGY+W    +
Sbjct:   83 RQRYDFTGTPGRKVDVSDLTDPLQYFELFFTEELVSKITRETNAQAALLASKPPGPKGFSRMDKWKDTDNDELKVFFAVMLLQGIVQKPELEMFWSTRPLLDTPYLRQIMTGERFLLLFRCLHFVNNSSISAGQSKAQISLQKIKPVFDFLVNKFSTVYTPNRNIAVDESLMLFKGPLAIKQYVPTKRVRFGLKLYVLCESQSGYVWNALVH 294

HSP 2 Score: 92.0485 bits (227), Expect = 7.664e-83
Identity = 64/191 (33.51%), Postives = 95/191 (49.74%), Query Frame = 3 
Query: 1638 VTRYCGK*MALKCHDKKDVSMMSTFHTAEVQDV---------LAIVVQEKSLWSSAITMPTWVLLMFRTRCLHHTQLKERDSKFGITELFRHLMNKVSLNCYLLFKK-QIENKLIHVQFRLKLIEWLIDVYHMPEHQADTGHTATAGGDPFRLTGCHFPTYGLVTAKKMNAQRICAVCSSK-EVDGKKIRR 2177
            V R+CG+ MALK  D K+V+M+STFH+  V +V            V+ + +    A+     +L  + +    H        K    + F HL++   LN Y+LFKK   E  + H+ FRL LIE +++ +H P  Q   G   +    P RL+G HFP     T+ K N    C +C S+ + DGKKIR+
Sbjct:  375 VARFCGELMALKWCDTKEVTMLSTFHSDTVIEVNNRNGKKTKKPCVIVDYNENMGAVDSADQMLTSYPSERKRH--------KVWYKKFFHHLLHITVLNSYILFKKDNPEYTMSHINFRLTLIERMLEKHHKPGQQHLRGRPCSDDVTPLRLSGRHFPKSIPPTSGKQNPTGRCKICCSQYDKDGKKIRK 557

HSP 3 Score: 77.0258 bits (188), Expect = 7.664e-83
Identity = 38/67 (56.72%), Postives = 49/67 (73.13%), Query Frame = 1 
Query: 1432 NNLETSKIVITLLQNLPRKVYTVYMDNYYTSPMLFRELISNNTDAVGTVRLNRKNIPADPKK*IVLG 1632
            + L++S+IV+TL+ +L  + Y V++DN+  SPMLFREL  N TDAVGT RLNRK IP D KK I  G
Sbjct:  306 DGLKSSRIVLTLVNDLLGQGYCVFLDNFNISPMLFRELHQNRTDAVGTARLNRKQIPNDLKKRIAKG 372

HSP 4 Score: 39.2762 bits (90), Expect = 7.664e-83
Identity = 14/28 (50.00%), Postives = 19/28 (67.86%), Query Frame = 1 
Query: 2176 ES*YESADCDVRLCAAPCFQIYHIIRKF 2259
            E+ Y  A+CDV LC  PCF+IYH  + +
Sbjct:  558 ETRYFCAECDVPLCVVPCFEIYHTQKNY 585

HSP 5 Score: 66.6254 bits (161), Expect = 1.748e-7
Identity = 28/39 (71.79%), Postives = 33/39 (84.62%), Query Frame = 2 
Query: 1757 EKPVVICDYNANMGAVDVSDQMLTSYPAERKRLEVWYNR 1873
            +KP VI DYN NMGAVD +DQMLTSYP+ERKR +VWY +
Sbjct:  416 KKPCVIVDYNENMGAVDSADQMLTSYPSERKRHKVWYKK 454
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. TrEMBL
Match: A0A2K5PIR4 (PiggyBac transposable element derived 4 OS=Cebus capucinus imitator OX=1737458 GN=PGBD4 PE=4 SV=1)

HSP 1 Score: 173.326 bits (438), Expect = 9.035e-83
Identity = 91/212 (42.92%), Postives = 130/212 (61.32%), Query Frame = 2 
Query:  818 QQQLPFSGTPGINVHISMPGNRLEYFGLFVTDHIVQSIVTETNRRHGLRFANP*-LKARSRL**WSITNPDEIKGFIAIILYQGLVQKPQTAMYWSTNLMLATPYIRRIMTND-------------NKTLSEAETNTEKSFYKIKKIFEAVYSRFSIIYKSGQNIAIDESLMI*KWILAKKQYILLKRARFGLKLYFLCESGSGYIWKEFTY 1411
            +Q+  F+GTPG  V +S   + L+YF LF T+ +V  I  ETN +  L  + P   K  SR+  W  T+ DE+K F A++L QG+VQKP+  M+WST  +L TPY+R+IMT +             N ++S  ++  + S  KIK +F+ + ++FS +Y   +NIA+DESLM+ K  LA KQY+  KR RFGLKLY LCES SGY+W    +
Sbjct:   83 RQRYDFTGTPGRKVDVSDVTDPLQYFELFFTEELVSKITRETNAQAALLASKPPGPKGFSRMDKWKDTDNDELKVFFAVMLLQGIVQKPELEMFWSTRPLLDTPYLRQIMTGERFLLLFRCLHFVNNSSISAGQSKAQISLQKIKPVFDFLVNKFSTVYTPNRNIAVDESLMLFKGPLAIKQYVPTKRVRFGLKLYVLCESQSGYVWNALVH 294

HSP 2 Score: 91.6633 bits (226), Expect = 9.035e-83
Identity = 64/191 (33.51%), Postives = 95/191 (49.74%), Query Frame = 3 
Query: 1638 VTRYCGK*MALKCHDKKDVSMMSTFHTAEVQDV---------LAIVVQEKSLWSSAITMPTWVLLMFRTRCLHHTQLKERDSKFGITELFRHLMNKVSLNCYLLFKK-QIENKLIHVQFRLKLIEWLIDVYHMPEHQADTGHTATAGGDPFRLTGCHFPTYGLVTAKKMNAQRICAVCSSK-EVDGKKIRR 2177
            V R+CG+ MALK  D K+V+M+STFH+  V +V            V+ + +    A+     +L  + +    H        K    + F HL++   LN Y+LFKK   E  + H+ FRL LIE +++ +H P  Q   G   +    P RL+G HFP     T+ K N    C +C S+ + DGKKIR+
Sbjct:  375 VARFCGELMALKWCDTKEVTMLSTFHSDTVIEVNNRNGKKTKKPRVIVDYNENMGAVDSADQMLTSYPSERKRH--------KVWYKKFFHHLLHITVLNSYILFKKDNPEYTMSHINFRLTLIERMLEKHHKPGQQHLRGRPCSDDVTPLRLSGRHFPKSIPPTSGKQNPTGRCKICCSQYDKDGKKIRK 557

HSP 3 Score: 76.6406 bits (187), Expect = 9.035e-83
Identity = 38/67 (56.72%), Postives = 49/67 (73.13%), Query Frame = 1 
Query: 1432 NNLETSKIVITLLQNLPRKVYTVYMDNYYTSPMLFRELISNNTDAVGTVRLNRKNIPADPKK*IVLG 1632
            + L++S+IV+TL+ +L  + Y V++DN+  SPMLFREL  N TDAVGT RLNRK IP D KK I  G
Sbjct:  306 DGLKSSRIVLTLVNDLLGQGYCVFLDNFNISPMLFRELHQNRTDAVGTARLNRKQIPNDLKKRIAKG 372

HSP 4 Score: 39.2762 bits (90), Expect = 9.035e-83
Identity = 14/28 (50.00%), Postives = 19/28 (67.86%), Query Frame = 1 
Query: 2176 ES*YESADCDVRLCAAPCFQIYHIIRKF 2259
            E+ Y  A+CDV LC  PCF+IYH  + +
Sbjct:  558 ETRYFCAECDVPLCVVPCFEIYHTQKNY 585

HSP 5 Score: 65.4698 bits (158), Expect = 4.039e-7
Identity = 28/39 (71.79%), Postives = 33/39 (84.62%), Query Frame = 2 
Query: 1757 EKPVVICDYNANMGAVDVSDQMLTSYPAERKRLEVWYNR 1873
            +KP VI DYN NMGAVD +DQMLTSYP+ERKR +VWY +
Sbjct:  416 KKPRVIVDYNENMGAVDSADQMLTSYPSERKRHKVWYKK 454
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Cavefish
Match: ENSAMXT00000043919.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02002039.1:240413:244210:1 gene:ENSAMXG00000040755.1 transcript:ENSAMXT00000043919.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 71.633 bits (174), Expect = 1.065e-20
Identity = 52/209 (24.88%), Postives = 92/209 (44.02%), Query Frame = 2 
Query:  869 MPG---NRLEYFGLFVTDHIVQSIVTETNRRHGLRFANP*LKARSRL**WSITNPDEIKGFIAIILYQGLVQKPQTAMYWSTNLMLATPYIRRIMTN-------DNKTLSEAETNTEK----------SFYKIKKIFEAVYSRFSIIYKSGQNIAIDESLMI*KWILAKKQYILLKRARFGLKLYFLCESGSGYIWKEFTYRTSNAFVT 1435
            +PG   + L+ F LF +  +VQ++V  TN+   +  A        +   W+     E   FI +++Y GLV+       WS   +    +   +M++        N  LS+ E + E             +KIK ++  + S     ++  + ++IDE ++  K     KQY+  K  ++G KL+ L +S SGY W  F Y   + F +
Sbjct:  169 LPGKTYSPLQLFQLFFSSSVVQTLVDNTNKFGKISAAG------GKKLKWTPVTVKEFYAFIGLVVYMGLVKVKSIMDLWSKKQLYQFQFPSMVMSSKRFTAISSNLHLSDPEEDAENMKKKGTPDYDRLFKIKPLYTDILSACHSFFQPRRELSIDERMVASKARTGLKQYMKAKPTKWGFKLFILADSASGYTWNFFVYEGKSPFAS 371

HSP 2 Score: 44.2838 bits (103), Expect = 1.065e-20
Identity = 19/33 (57.58%), Postives = 24/33 (72.73%), Query Frame = 1 
Query: 1492 YTVYMDNYYTSPMLFRELISNNTDAVGTVRLNR 1590
            Y VYMDN+YTSP L  +L+  NT A GT+R N+
Sbjct:  393 YMVYMDNFYTSPTLLSDLLQENTLACGTLRTNQ 425

HSP 3 Score: 24.2534 bits (51), Expect = 1.065e-20
Identity = 16/51 (31.37%), Postives = 27/51 (52.94%), Query Frame = 3 
Query: 1653 GK*MALKCHDKKDVSMMSTFHTAEVQDVLAIVVQEK-SLWS-SAITMPTWV 1799
            GK + +K  D ++V+M ST H A   D +   V+ K  +W  + + +P  V
Sbjct:  451 GKLLYVKWMDTREVTMCSTMHKAYKGDTVKRKVKSKEGVWDITTVPIPAAV 501
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Cavefish
Match: ENSAMXT00000014953.2 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02001957.1:155693:158756:-1 gene:ENSAMXG00000014528.2 transcript:ENSAMXT00000014953.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 68.1662 bits (165), Expect = 2.232e-18
Identity = 44/190 (23.16%), Postives = 94/190 (49.47%), Query Frame = 2 
Query:  842 TPGINVHISMPGNRLEYFGLFVTDHIVQSIVTETNRRHGLRFANP*LKARSRL**WSITNPDEIKGFIAIILYQGLVQKPQTAMYWSTNLMLATPYIRRIMTNDNKT-------LSEAETNTEKSFYKIKKIFEAVYSRFSIIYKSGQNIAIDESLMI*KWILAKKQYILLKRARFGLKLYFLCESGSGY 1390
            TPG+ +      +  + F LF +  +++++ + TN+ +  R +   +K +     W+  N DE+  F++I +Y GL++   T   W  + + + P+   +M             +S+ + + +    + +  ++ ++ R    Y+  QN++IDE ++  K  +  KQY+  K  ++G KL+ L +S SGY
Sbjct:   89 TPGVQLDFQTDYSASDIFQLFFSKEVIRTLCSNTNK-YAARRSVQGIKRQ-----WTDVNADEMLKFLSITIYMGLMKPSATRDLWRKDRLHSHPFPASVMAGYRYEAISAYLHMSDPDADVKNDQLRGQPEYDGLF-RLKAYYQPHQNLSIDERMVATKARIRMKQYMKDKPTKWGYKLFILADSCSGY 271

HSP 2 Score: 44.669 bits (104), Expect = 2.232e-18
Identity = 24/47 (51.06%), Postives = 27/47 (57.45%), Query Frame = 1 
Query: 1492 YTVYMDNYYTSPMLFRELISNNTDAVGTVRLNRKNIP-----ADPKK 1617
            YTVY+DN+YTS MLFR L      A GT+R  R   P     A PKK
Sbjct:  308 YTVYVDNFYTSSMLFRHLHRIGFGACGTIRETRIGFPKTTANALPKK 354
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Cavefish
Match: si:ch211-130m23.5 (piggyBac transposable element-derived protein 4-like [Source:NCBI gene;Acc:103027433])

HSP 1 Score: 80.8777 bits (198), Expect = 4.389e-18
Identity = 59/244 (24.18%), Postives = 106/244 (43.44%), Query Frame = 2 
Query:  728 VDYESHATVDYYHDVVDEETFHLN--NAPQQIQQQLPFSGTPGINVHISMPGNRLEYFGLFVTDHIVQSIVTETN-------RRHGLRFANP*LKARSRL**WSITNPDEIKGFIAIILYQGLVQKPQTAMYWSTNLMLATPYIRRIMT-------NDNKTLSEAETNTEKS------FYKIKKIFEAVYSRFSIIYKSGQNIAIDESLMI*KWILAKKQYILLKRARFGLKLYFLCESGSGYI 1393
            ++ E HA +   +DV+D+E    +    P Q+    PF+   G    +    + L+YF L   D + + +V +TN       RR G    +           W  T+  E++ ++ + +  G+ Q P   MYW++++ +     ++ MT       N N  L +  +   K        YKI+ + + V +     Y   + + +DE  +  K   +  QY+  K  R GLK++ LC+S SGY 
Sbjct:  148 LEEEEHAGLFAPYDVLDDECTSPDFWGEPDQVISIEPFTADCGPQHSLGDDADTLDYFRLLFPDSLFEHMVEQTNNYALYRQRRSGRSDPH-----------WHPTDVREMRAYVGLNILMGINQLPDYGMYWASDIFIGNAGFKKTMTARRFEKLNQNLHLCDRLSEPSKGELGYDGLYKIRPLLDMVGNTMWDSYLPNRCLTVDECAIATKGRFSPTQYMPSKPLRKGLKVWMLCDSRSGYC 380

HSP 2 Score: 30.8018 bits (68), Expect = 4.389e-18
Identity = 17/63 (26.98%), Postives = 36/63 (57.14%), Query Frame = 1 
Query: 1450 KIVITLLQNLPRKVYTVYMDNYYTSPMLFRELISNNTDAVGTVRLNRKNIPADPKK*IVLGKL 1638
            ++V++L++ L  + + ++MD+++TS  L + L+ +   + G     R+  P D  K   +GKL
Sbjct:  402 RMVMSLVRGLEGRYHHLFMDSFFTSVPLLQRLLQDGIYSCGPTNPYRRGYP-DALKPRNVGKL 463
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Cavefish
Match: ENSAMXT00000057483.1 (piggyBac transposable element-derived protein 4-like [Source:NCBI gene;Acc:107197084])

HSP 1 Score: 68.5514 bits (166), Expect = 7.779e-18
Identity = 46/200 (23.00%), Postives = 93/200 (46.50%), Query Frame = 2 
Query:  842 TPGINVHISMPGNRLEYFGLFVTDHIVQSIVTETNRRHGLRFANP*LKARSRL**WSITNPDEIKGFIAIILYQGLVQKPQTAMYWSTNLMLATPYIRRIMTNDNKT-------LSEAETNTEK----------SFYKIKKIFEAVYSRFSIIYKSGQNIAIDESLMI*KWILAKKQYILLKRARFGLKLYFLCESGSGY 1390
            TPG+ +      +  E F LF +  +++++   TN+ +  R +   +K +     W   N DE+  F++II+Y GL++       W  + + + P+   +M             +S+ +++ +             +++K + E + S     Y+  QN++IDE ++  K  +  KQY+  K  ++G  L+ L +S SGY
Sbjct:  141 TPGVQLDFQTDYSASEIFQLFFSKEVIRTLCINTNK-YAARRSEEGMKKQ-----WKDVNADEMLHFLSIIIYMGLLKPSAVRDLWRKDRLHSHPFPASVMAGYRYGDIAAYLHMSDPDSDAKNDQLRGEPEYDGLFRLKPLQEQILSACRAYYQPHQNLSIDERMVATKARIGMKQYMKNKPTKWGYMLFVLADSSSGY 334

HSP 2 Score: 39.6614 bits (91), Expect = 7.779e-18
Identity = 18/37 (48.65%), Postives = 22/37 (59.46%), Query Frame = 1 
Query: 1492 YTVYMDNYYTSPMLFRELISNNTDAVGTVRLNRKNIP 1602
            YTVY+DN+YTS +LF  L      A GT+R  R   P
Sbjct:  371 YTVYVDNFYTSSLLFHHLHRIGFGACGTIRETRIGFP 407

HSP 3 Score: 22.3274 bits (46), Expect = 7.779e-18
Identity = 40/189 (21.16%), Postives = 76/189 (40.21%), Query Frame = 3 
Query: 1653 GK*MALKCHDKKDVSMMSTFHTA-EVQDVLAIVVQEKSLWSS-AITMPTWVL--------LMFRTRCLHHTQLKERDSKFGITELFRHLMNKVSLNCYLLFKK----QIENKLIHVQFRLKLIEWLIDVYH----MP-EHQADTGHTATAGGDPFRLTGCHFPTYGLVTA----KKMNAQRICAVCSSK 2150
            G  + +K  D +DV++ ST H A   Q V   +  +   WS+  I +P  V         +      + +  + ++  ++ + +LF H ++   +N +++ K+    + +  L    FR  L   L DV      +P E  ++T  ++       +  GC       V+A    K    +R C  C  K
Sbjct:  429 GSLLFVKWRDTRDVTVCSTVHKAYSGQSVQRRIRIKNGTWSTQQIPVPEPVKAYNKFMGGVDLSDALIKYYSVTQKTRRWYV-KLFLHFVDIAVVNSFIIHKEMALARKQKPLSQKSFREVLCMELADVGQPQVSIPAEVPSETSSSSVPQDKELKPKGCFPVPVSEVSAENRMKATQGRRKCVQCKMK 616
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Cavefish
Match: ENSAMXT00000030524.1 (pep primary_assembly:Astyanax_mexicanus-2.0:8:5821371:5823572:-1 gene:ENSAMXG00000031777.1 transcript:ENSAMXT00000030524.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 57.3806 bits (137), Expect = 1.043e-15
Identity = 42/200 (21.00%), Postives = 84/200 (42.00%), Query Frame = 2 
Query:  842 TPGINVHISMPGNRLEYFGLFVTDHIVQSIVTETNRRHGLRFANP*LKARSRL**WSITNPDEIKGFIAIILYQGLVQKPQTAMYWSTNLMLATPYIRRIMTND-------NKTLSEAE----------TNTEKSFYKIKKIFEAVYSRFSIIYKSGQNIAIDESLMI*KWILAKKQYILLKRARFGLKLYFLCESGSGY 1390
            TPG  +  +     L+ F LF     VQ++   TN++     +        +   W+  + +E   +  ++LY  L++    + YW  + + +     +IM+ D       N  LS+ +          T      +++K + E + +     Y   + +A+DE ++  K      QY+  K  ++G KL+ L +S +GY
Sbjct:  173 TPGPQLISASSYTPLDLFKLFFPVQTVQTLCQNTNKQAAKNLSE------GKKYKWTDLDAEEFYKYCGLVLYMALIKVDSVSDYWRQDHLFSLSLPSQIMSRDRYRTVSWNIHLSDPDEDVHNDQKKGTAQHDKLFRLKPLMETIKNACKAFYHPHKELAVDERMVASKAKTGMTQYMKSKPTKWGFKLFVLADSRNGY 366

HSP 2 Score: 46.2098 bits (108), Expect = 1.043e-15
Identity = 20/37 (54.05%), Postives = 23/37 (62.16%), Query Frame = 1 
Query: 1492 YTVYMDNYYTSPMLFRELISNNTDAVGTVRLNRKNIP 1602
            Y +YMDN+Y+SP LFR L      A GT R NRK  P
Sbjct:  403 YHIYMDNFYSSPKLFRALHECKFSACGTYRENRKGCP 439
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002004.1 (pep scaffold:Pmarinus_7.0:GL490362:1254:2954:1 gene:ENSPMAG00000001819.1 transcript:ENSPMAT00000002004.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 75.0998 bits (183), Expect = 6.023e-20
Identity = 48/199 (24.12%), Postives = 90/199 (45.23%), Query Frame = 2 
Query:  833 FSGTPGINVHISMPGNRLEYFGLFVTDHIVQSIVTETNRRHGLRFANP*LKARSRL**WSITNPDEIKGFIAIILYQGLVQKPQTAMYWSTNLMLATPYIRRIMT-----------NDNKTLSEAETNTEKSFYKIKKIFEAVYSRFSIIYKSGQNIAIDESLMI*KWILAKKQYILLKRARFGLKLYFLCESGSGYIW 1396
            F+  PG+   +  P   L +  LF+T  ++     ETN       AN   + R+    W   N  +I  ++ + +  G+   P+  MYWST    + P  R  M+           N + + +    NT++   +++ + E   S+F  +Y   + +++DE+ +  K  L  K Y   K  ++G+K Y L E+ +GY++
Sbjct:    2 FTAVPGMQTPV--PATPLGFIQLFITRELLMFFTNETNSYANCALANKSAEERAG---WEGVNLMDIANYLGLTMAMGVASLPEIPMYWSTGKPYSMPAFRETMSCKRFKSIGRFFNCHNSCAVPRDNTDR-LLQVRTVMEYFQSKFKALYAPNRELSLDEATLEWKGRLPFKVYNPQKPEKYGIKFYILAEAQTGYVF 194

HSP 2 Score: 40.817 bits (94), Expect = 6.023e-20
Identity = 25/62 (40.32%), Postives = 31/62 (50.00%), Query Frame = 1 
Query: 1453 IVITLLQNLPRKVYTVYMDNYYTSPMLFRELISNNTDAVGTVRLNRKNIPADPKK*IVLGKL 1638
            IV  L+  L  K Y +YMDNYY S  L  EL  +     GT+RL+R      PK    L K+
Sbjct:  209 IVFGLMGPLLDKGYQLYMDNYYNSVKLTEELYEHGVHTCGTLRLSR----GAPKDLQALAKM 266
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007705.1 (pep scaffold:Pmarinus_7.0:GL484572:34:4229:-1 gene:ENSPMAG00000006959.1 transcript:ENSPMAT00000007705.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 65.855 bits (159), Expect = 8.779e-17
Identity = 39/143 (27.27%), Postives = 69/143 (48.25%), Query Frame = 2 
Query: 1007 WSITNPDEIKGFIAIILYQGLVQKPQTAMYWSTNLMLATPYIRRIMT--------------NDNKTLSEAETNTEKSFYKIKKIFEAVYSRFSIIYKSGQNIAIDESLMI*KWILAKKQYILLKRARFGLKLYFLCESGSGYI 1393
            WS T   E+K F A+ +  G+   P+   YWS N  L   ++   MT              N+  T +  +   +   YK++ + E V + FS  Y   + +++DE ++  K  L  KQ++  K  ++G+K++ LCE+ +GY 
Sbjct:    1 WSPTTSREMKAFFAVAIMMGIKDLPRVWCYWSGNPALRCEWVSGTMTHTRYSKVSQYLHARNNVGTPARGQRGYD-PIYKVRNVVEEVRANFSRHYSPRRELSVDEGMIPFKGRLFFKQHMPAKPTKWGVKVWELCEAQTGYC 142

HSP 2 Score: 39.2762 bits (90), Expect = 8.779e-17
Identity = 18/52 (34.62%), Postives = 33/52 (63.46%), Query Frame = 1 
Query: 1450 KIVITLLQNLPRKVYTVYMDNYYTSPMLFRELISN-NTDAVGTVRLNRKNIP 1602
            ++V++L ++   + + +Y D Y++SP L  +L S   T A GTV L+R+ +P
Sbjct:  167 RVVMSLTRSYWHRNHHLYFDRYFSSPALLEDLRSRCGTYACGTVSLSRRGLP 218
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001816.1 (pep scaffold:Pmarinus_7.0:GL492440:267714:269431:-1 gene:ENSPMAG00000001650.1 transcript:ENSPMAT00000001816.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 54.299 bits (129), Expect = 6.918e-8
Identity = 51/214 (23.83%), Postives = 88/214 (41.12%), Query Frame = 2 
Query:  833 FSGTPGINVHISMPGNR-LEYFGLFVTDHIVQSIVTETNRRHGLRFANP*LKARSRL**WSITNPDEIKG------------FIAIILYQGLVQKPQTAMYWSTNLMLATPYIRRI--------------MTNDNKTLSEAETNTEKSFYKIKKIFEAVYSRFSIIYKSGQNIAIDESLMI*KWILAKKQYILLKRARFGLKLYFLCESGSGYI 1393
            F G+PG  VH    G++ L+YF LF+TD ++  IVTETNR      AN  L             P  + G            F+ ++L  G+   P    +W+ +  L   +IRR               +++D K  S      +   +KI+ + + +   FS  +   +++ +DE L   K        +     R G+K++    + +GY+
Sbjct:    5 FQGSPG-PVHALPAGSQPLDYFKLFLTDEMLDRIVTETNRHAARCGANMHLTG---------PGPGSVSGEGRPVTRSSLSTFLGVVLIMGINMLPALPYHWADDDSLGNQFIRRSISHKRFSFLQRYLHISDDEKAPSRGRIGYD-PLHKIRPLLDHLLGAFSRHFVPFRDMRLDEVLSKFKDRHDALPCVPANPIRCGVKVFICSCASTGYV 207
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Medaka
Match: si:ch211-130m23.5 (piggyBac transposable element-derived protein 4-like [Source:NCBI gene;Acc:105354747])

HSP 1 Score: 70.0922 bits (170), Expect = 6.346e-16
Identity = 52/231 (22.51%), Postives = 93/231 (40.26%), Query Frame = 2 
Query:  767 DVVDEETFHLN--NAPQQIQQQLPFSGTPGINVHISMPGNRLEYFGLFVTDHIVQSIVTETN-------RRHGLRFANP*LKARSRL**WSITNPDEIKGFIAIILYQGLVQKPQTAMYWSTNLMLATPYIRRIMT-------------NDNKTLSEAETNTEKSFYKIKKIFEAVYSRFSIIYKSGQNIAIDESLMI*KWILAKKQYILLKRARFGLKLYFLCESGSGYI 1393
            D +DE+    +    P Q+    PFS   G    +    +  +YF L   D + + +V +TN       RR G    +           W  T+  E+K ++ + +  G+ Q P T MYW++++ +     ++ MT              D +             YKI+ + + V +     Y   + + ID+  +  K   +  QY+  K  + GL ++ LC+S SGY 
Sbjct:  157 DDLDEDCASPDFWGEPDQVISIEPFSAASGPQHSLGDDADTRDYFRLLFPDSLFEHMVEQTNNYALYRQRRSGKSDPH-----------WHPTDVREMKAYVGLNILMGINQLPDTGMYWASDIFIGNAGFKKTMTARRFEKLTQYLQLCDREAEPVRGERCYDGLYKIRPLLDVVENTMWDTYTPNRCLTIDKCSIATKGRFSPTQYMSSKPVKKGLTVWMLCDSRSGYC 376

HSP 2 Score: 34.2686 bits (77), Expect = 6.346e-16
Identity = 14/51 (27.45%), Postives = 30/51 (58.82%), Query Frame = 1 
Query: 1450 KIVITLLQNLPRKVYTVYMDNYYTSPMLFRELISNNTDAVGTVRLNRKNIP 1602
            ++V  L++ L R+ + ++MD+++TS  L + L+ +   A G  +  R+  P
Sbjct:  398 RVVTFLVRGLERQYHHLFMDSFFTSVPLLQRLLRDGLYACGPTQPGRRGYP 448
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Medaka
Match: ENSORLT00000021107.2 (piggyBac transposable element-derived protein 4 [Source:NCBI gene;Acc:101163734])

HSP 1 Score: 44.2838 bits (103), Expect = 1.208e-10
Identity = 29/141 (20.57%), Postives = 60/141 (42.55%), Query Frame = 2 
Query: 1007 WSITNPDEIKGFIAIILYQGLVQKPQTAMYWSTNLMLATPYIRRIMTNDN---------------KTLSEAETNTEK--SFYKIKKIFEAVYSRFSIIYKSGQNIAIDESLMI*KWILAKKQYI-LLKRARFGLKLYFLCE 1375
            W    P E+  ++ ++LY  +   P+   YW  N +    Y   +M+ D                  ++E +  TE+     +++ + E + SR    Y   Q+I++ E ++  K  L  K+++   K  ++GL  + L +
Sbjct:  165 WKDLTPQELTKYLGLLLYMSVCNFPRITDYWRKNSIFHVSYPAAVMSRDRFLTISSHLHFSDPDVDAVNEQKMGTEEYDPLQRVRCLLEMISSRCKAAYHPRQHISVGERMVATKGRLQIKEHVDADKPTKWGLNFFVLAD 305

HSP 2 Score: 42.3578 bits (98), Expect = 1.208e-10
Identity = 17/37 (45.95%), Postives = 22/37 (59.46%), Query Frame = 1 
Query: 1492 YTVYMDNYYTSPMLFRELISNNTDAVGTVRLNRKNIP 1602
            Y VY D++YTSP+LFR LI     A G  +  R  +P
Sbjct:  346 YIVYCDSFYTSPVLFRHLIQQGFGACGMYKQGRTGVP 382
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Medaka
Match: ENSORLT00000029842.1 (piggyBac transposable element-derived protein 4 [Source:NCBI gene;Acc:101157644])

HSP 1 Score: 50.8322 bits (120), Expect = 2.410e-9
Identity = 39/192 (20.31%), Postives = 79/192 (41.15%), Query Frame = 2 
Query:  884 LEYFGLFVTDHIVQSIVTETNRR-----HGLRFANP*LKARSRL**WSITNPDEIKGFIAIILYQGLVQKPQTAM--YW---------STNLMLATPYIRRIMTNDNKTLSEAETNTEKSFYKIKKIFEAVYSRFSIIYKSGQNIAIDESLMI*KWILAKKQYILLKRARFGLKLYFLCESGSGYIWKEFTY 1411
            L  F LF T ++ + I+  TNR      H L+   P              +  E++ ++ +++  G+ +    A+   W            + L + Y R      +   + A  +       +++++E    R   +Y  G  + +DE ++  K   + +QYI  K  ++GLKL+  C++ + Y W+   Y
Sbjct:  371 LSSFLLFFTPNVERLILIATNRHSADKAHALKTPKP-------------MDEIELRAYVGLLILAGVYRSRGEALVSLWEPECGRPIFGATMSLRSFYQRSATLRFDDRSTRASRSASDRLAAVREVWEEWCDRLPRLYDIGPEVTVDERMVPFKGRCSFRQYIPSKPTKYGLKLWVACDARTSYAWRVQPY 549

HSP 2 Score: 31.5722 bits (70), Expect = 2.410e-9
Identity = 17/55 (30.91%), Postives = 30/55 (54.55%), Query Frame = 1 
Query: 1444 TSKIVITLLQNLPRKVYTVYMDNYYTSPMLFRELISNNTDAVGTVRLNRKNIPAD 1608
             S++V  L + L  +   V  DN++TS  L  EL+  N   +GT++ N+  +P +
Sbjct:  563 ASRVVSDLTRGLEHR--NVTCDNFFTSYALATELLKRNITLLGTIKSNKPELPPE 615
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Medaka
Match: ENSORLT00000039079.1 (pep primary_assembly:ASM223467v1:3:33786070:33791146:1 gene:ENSORLG00000029902.1 transcript:ENSORLT00000039079.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 47.7506 bits (112), Expect = 2.937e-8
Identity = 49/190 (25.79%), Postives = 87/190 (45.79%), Query Frame = 2 
Query:  884 LEYFGLFVTDHIVQSIVTETNRRHGLRFANP*LKARSRL**WSITNPDEIKGFIAIILYQGLVQKPQTAMYWS--------TNLMLATPY--IRRIMT-NDNKTLSEAETNTEKSFYKIKKIFEAVYSRFSIIYKSGQNIAIDESLMI*KWILAK--KQYILLKRARFGLKLYFLCESGSGYIWKEFTYR 1414
             +YF  +  D I++ I  +TN     +  N    A          N +EI  F+ I++Y G+V+ P    YW+         NLM +  +  ++R++  NDN  +      T   F+KI+++F  + + F    ++ +  ++DE ++  K   A   +QYI  K  ++G KL F   S  G+I     Y+
Sbjct:  126 FQYFTRYFDDQIIRHITYQTNLYAAQKDINITFTA----------NENEILTFVTILIYMGIVELPSVDDYWARETRVPQVANLMSSKRFRLLKRVVHFNDNTMI----YGTSDRFFKIRQLFSYINTAFRRESQTPKQ-SVDEVMVAYKGKKAGNLRQYIKSKPDKWGFKL-FARASEDGFIHDMVLYQ 299

HSP 2 Score: 30.8018 bits (68), Expect = 2.937e-8
Identity = 20/54 (37.04%), Postives = 27/54 (50.00%), Query Frame = 1 
Query: 1444 TSKIVITLLQNLPRKVY-TVYMDNYYTSPMLFRELISNNTDAVGTVRLNRKNIP 1602
            TS+IV  L+  +       +Y DN++TS  L R L  +N    GT R NR   P
Sbjct:  321 TSQIVSVLVSTMSFPTTPAIYADNFFTSLELVRYLRDHNCRYTGTARENRIGKP 374
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Medaka
Match: ENSORLT00000037833.1 (piggyBac transposable element-derived protein 4-like [Source:NCBI gene;Acc:101160710])

HSP 1 Score: 46.2098 bits (108), Expect = 4.806e-8
Identity = 39/185 (21.08%), Postives = 80/185 (43.24%), Query Frame = 2 
Query:  893 FGLFVTDHIVQSIVTETNRRHGLRFANP*LKARSRL**WSITNPDEIKGFIAIILYQGLVQKP--QTAMYWS---------TNLMLATPY-IRRIMTNDNKTLSEAETNTEKSFYKIKKIFEAVYSRFSIIYKSGQNIAIDESLMI*KWILAKKQYILLKRARFGLKLYFLCESGSGYIWKEFTY 1411
            F LF++  I + I+  TN      F             W   +  ++  +I I++  G+ +     T+  W+           + L T + I R++  DN+        ++K    I+ +++       ++Y  G ++ +DE L+  +     +QY+  K A++G+K++  C++ S Y WK   Y
Sbjct:  125 FQLFISQPIERIILDMTNLEGRRVFQEK----------WKSLDQTDLNAYIGILILAGVYRSKGEATSSLWNEENGRPIFRATMSLETFHMISRVIRFDNRDTRVGRRESDK-LAAIRDVWDKWVEILPLLYNPGPHVTVDERLVPFRGRCPFRQYMPNKPAKYGIKIWAACDAKSSYAWKMQVY 298

HSP 2 Score: 31.187 bits (69), Expect = 4.806e-8
Identity = 18/65 (27.69%), Postives = 36/65 (55.38%), Query Frame = 1 
Query: 1450 KIVITLLQNLPRKVYTVYMDNYYTSPMLFRELISNNTDAVGTVRLNRKNIPADPKK*IVLGKLLH 1644
            ++V+ + + L  + + +  DN++TS  L  EL       +GTVR N+  +P++  K  + G+ +H
Sbjct:  314 RVVLEMSEGL--QGHNITCDNFFTSYRLGEELQKRKLTMLGTVRRNKPELPSEILK--IQGRPMH 374
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. Planmine SMEST
Match: SMESG000067117.1 (SMESG000067117.1)

HSP 1 Score: 77.7962 bits (190), Expect = 3.802e-21
Identity = 55/181 (30.39%), Postives = 88/181 (48.62%), Query Frame = 2 
Query:  884 LEYFGLFVTDHIVQSIVTETN---RRHGLRFANP*LKARSRL**WSITNPDEIKGFIAIILYQGLVQKPQTAMYWSTNLMLATPYIRRIMTNDNKTL--------SEAETNTEKSFYKIKKIFEAVYSRFSIIYKSGQNIAIDESLMI*KWILAKKQYILLKRARFGLKLYFLCESGSGYI 1393
            ++YF  F+    +  +V ETN   ++H L        ++ ++  W     +E+  F AI++  GLV K +   YWS + +L TP   +  T  N+ L        +  E  T    +KI  +   V  +F  +    + I IDESLM+ K  L+ KQYI LKR RFG+KL+ + +  +  I
Sbjct:   69 IDYFEWFIDSEFMDYVVKETNLYQQQHPL-------PSQKKMAKWFDVTSNELSVFFAIVMLTGLVSKNELKDYWSVDPLLNTPIFPKTFTR-NRFLQIMRFLHFANNEQKTNDKLFKIGNVVNMVKEKFKNVIIPDRLICIDESLMLWKGRLSFKQYIPLKRHRFGIKLFEMVDCTTQCI 241

HSP 2 Score: 43.8986 bits (102), Expect = 3.802e-21
Identity = 21/53 (39.62%), Postives = 32/53 (60.38%), Query Frame = 1 
Query: 1444 TSKIVITLLQNLPRKVYTVYMDNYYTSPMLFRELISNNTDAVGTVRLNRKNIP 1602
            +  IV+ L+     K + +Y++N+Y+SP LF  L    T A GT+R NRK +P
Sbjct:  263 SGSIVMELMNMHLDKGHVLYVENWYSSPPLFEILHYKKTGACGTLRKNRKGLP 315
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. Planmine SMEST
Match: SMESG000071608.1 (SMESG000071608.1)

HSP 1 Score: 70.0922 bits (170), Expect = 3.718e-14
Identity = 61/240 (25.42%), Postives = 103/240 (42.92%), Query Frame = 2 
Query:  800 NAPQQIQQQLPFSGTPGINVHISMPGNRLEYFGLFVTDHIVQSIVTETNRRHGLRFANP*LKARSRL**WSITNPDEIKGFIAIILYQGLVQKPQTAMYWSTNLMLATPYIRRIMTNDNKTLSEAE--------TNTEKS-----FYKIKKIFEAVYSRFSIIYKSGQNIAIDESLMI*KWILAKKQYILLKRARFGLKLYFLCESGSGYIWKEFTYRTSNAFVTI*KHRKSSSLYCKTY 1480
            NA +   +   F G  G+ + +    +  + F LF  + ++  IV ETN               +RL  W   N +EI+ F  + ++ GLV+  +   YWS   +        IM+      S  E        +N E +       KIK + + + +++  ++  G+N  IDE+L+  +  L  +QYI  K  ++G+KL+ LC S  GY W    Y   +       + K+S L  K Y
Sbjct:   86 NAYECRHKCFDFIGKSGLQLDLPKNISAFDTFMLFFDEEVINLIVIETNIYAEQTIERTRTSRHNRLNKWHPANSEEIRKFFGLFMWMGLVRMGKITSYWSNKDIYKNSTALSIMSR-----SRFEILLRLLHFSNNENAIPGDRLAKIKPLLDLLEAKYKKVFVPGENHVIDETLVPWRGRLVFRQYIPNKAHKYGIKLFKLCTS-EGYTWASKMYSGKSEI-----YDKNSGLAAKVY 314

HSP 2 Score: 28.1054 bits (61), Expect = 3.718e-14
Identity = 17/44 (38.64%), Postives = 23/44 (52.27%), Query Frame = 1 
Query: 1477 LPRKVYTVYMDNYYTSPMLFRELISNNTDAVGTVRLNRKNIPAD 1608
            L  KVY     N+YTS  L  + +   T  V TVR N+K +P +
Sbjct:  309 LAAKVY-----NFYTSYPLALDFLRQKTHVVSTVRANKKFMPKE 347
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. Planmine SMEST
Match: SMESG000047155.1 (SMESG000047155.1)

HSP 1 Score: 51.6026 bits (122), Expect = 5.774e-14
Identity = 50/216 (23.15%), Postives = 99/216 (45.83%), Query Frame = 2 
Query:  776 DEETFHLNNAPQQIQQQLPFSGTPGINVHISMPGNRLEYFGLFVTDHIVQSIVTETNRRHGLRFANP*LKAR-----SRL**WSITNPDEIKGFIAIILYQGLVQKPQTAMYWSTNLMLATPYIRRIMTNDN-----KTLSEAETNTEKSFYKIKKIFEAVYSRFSIIYKSGQNIAIDESLMI*KWILAKKQYILLKRARFGLKLYFLCESGSGYI 1393
            ++ET+H N     I+     +   GI + I    + ++ F     D +++ I+  TN  +G++  N   +AR     SR   +  T+  E++ F+ + L Q  ++       +S + +   P     M+        +     E N+     KIK   + +  +F+  Y   + +++DESL++ +  L  + Y+  K+A++G+K Y LC S  GY+
Sbjct:   60 NDETWHTNTT--SIRNFSFDADAAGILLDIEENVSPIQVFEKIWDDQVMEMIIKSTND-YGIKLCN---QARPHTRHSRKANFKNTDMIEMRKFLGLCLLQSQLKFSSIRKLFSIDALHYHPAFPSQMSGRRFEQILRCFCVEEENSNDPLRKIKIFLDLLIQKFANAYAPNEALSLDESLLLFRGRLYFRTYMKGKKAKYGIKFYELC-SAQGYV 268

HSP 2 Score: 45.8246 bits (107), Expect = 5.774e-14
Identity = 21/52 (40.38%), Postives = 34/52 (65.38%), Query Frame = 1 
Query: 1453 IVITLLQNLPRKVYTVYMDNYYTSPMLFRELISNNTDAVGTVRLNRKNIPAD 1608
            +V+ L+Q+   K + ++MDNYY S  L  +L+S  T   GT+R+NRK +P +
Sbjct:  290 LVMRLMQSYLNKGHHLFMDNYYNSLPLSNKLLSKKTHVTGTLRVNRKGLPQE 341
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. Planmine SMEST
Match: SMESG000020543.1 (SMESG000020543.1)

HSP 1 Score: 56.225 bits (134), Expect = 4.533e-8
Identity = 76/291 (26.12%), Postives = 125/291 (42.96%), Query Frame = 2 
Query: 1064 GLVQKPQTAMYWST----NLMLATPYIRRIMTNDNKTL--------SEAETNTEKSFYKIKKIFEAVYSRFSIIYKSGQNIAIDESLMI*KWILAKKQYILLKRARFGLKLYFLCESGSGYIWKEFTYRTSNAFVTI*KHRKSSSLY---CKTYLGKFIQFTWTITTHLQCCLEN*YQIILMQLGLYV*IERIFQQIPKSK*FLVNCYTLLRKIDGLEVSRQK-GCFNDVNIXXXXXXXXXXXXG----TREKPVVICDYNANMGAVDVSDQMLTSYPAERKRLEVWYNRI 1876
            GLV+ P+  +YWS     NL L     R IM  +   L        +  E + +    K+  I   + + F I YK  + + +DESLM  +  +  +QYI  KR R+ +K++ L   G GY +    Y   +         KS+ +    C+  LGK      TI      C +N Y  + +        ER+  +  +    L+      R+ +  EV  QK     DV +  +  S   +       ++EKP ++ +YN    AVD+SDQM+ +Y +  +R   WY ++
Sbjct:    2 GLVRLPKIKLYWSKYEGYNLTLP----RTIMPRNRFELLLRFVHFSNNEENDPKNKLSKVDPIINHLNNNFKIYYKPDEVLCVDESLMPFRGRIVFRQYIKQKRHRYVIKVFKL-YIGPGYTYSFRIYTCKDENTNEISGNKSTEIVMALCQDILGK----GHTI------CTDNWYTSVDLA-------ERLIDKRTQ----LLGTLRKYRRGNPKEVVAQKLKHKRDVLMLSTKHSTEMTIIQKINFSKEKPKMVFEYNLGKSAVDLSDQMV-AYSSPLRRTIKWYKKL 265
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. Planmine SMEST
Match: SMESG000056413.1 (SMESG000056413.1)

HSP 1 Score: 53.5286 bits (127), Expect = 1.082e-7
Identity = 34/100 (34.00%), Postives = 51/100 (51.00%), Query Frame = 2 
Query:  827 LPFSGTPGINVHISMPGNRLE---YFGLFVTDHIVQSIVTETN-----RRHGLRFANP*LKARSRL**WSITNPDEIKGFIAIILYQGLVQKPQTAMYWS 1102
            + FS + GIN   S     +E   ++ LFV D I + I  +TN     ++ G        +   RL  W  TNP+EIK F  +I++ GLV+ P+  +YWS
Sbjct:  107 ITFSDSSGINTCQSEIFACMEPHNFYSLFVNDEIFEIIAKQTNIYVTQKKSG--------QQSHRLDEWYQTNPNEIKRFFGLIIWMGLVRLPKIELYWS 198
The following BLAST results are available for this feature:
BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
PGBD45.210e-8642.92piggyBac transposable element derived 4 [Source:HG... [more]
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BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
pgbd41.577e-2327.05piggyBac transposable element derived 4 [Source:ZF... [more]
pgbd41.542e-2127.05piggyBac transposable element derived 4 [Source:ZF... [more]
CABZ01069162.12.535e-1824.24pep scaffold:GRCz11:KN149950.1:74:5493:1 gene:ENSD... [more]
si:ch211-130m23.54.729e-1824.40si:ch211-130m23.5 [Source:ZFIN;Acc:ZDB-GENE-131121... [more]
si:dkey-19f4.21.844e-1424.36si:dkey-19f4.2 [Source:ZFIN;Acc:ZDB-GENE-121214-26... [more]
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BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSXETT00000026903.17.362e-3534.50pep primary_assembly:Xenopus_tropicalis_v9.1:4:203... [more]
ENSXETT00000040662.17.473e-3534.50pep primary_assembly:Xenopus_tropicalis_v9.1:2:103... [more]
ENSXETT00000029105.18.598e-3534.50pep primary_assembly:Xenopus_tropicalis_v9.1:3:719... [more]
epc11.111e-3434.50enhancer of polycomb homolog 1 [Source:Xenbase;Acc... [more]
ENSXETT00000042643.11.111e-3434.50pep primary_assembly:Xenopus_tropicalis_v9.1:6:703... [more]
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BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of PiggyBac transposable element-derived protein 4 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 1
Match NameE-valueIdentityDescription
sp|Q96DM1|PGBD4_HUMAN2.296e-8542.92PiggyBac transposable element-derived protein 4 OS... [more]
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BLAST of PiggyBac transposable element-derived protein 4 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
G3QWT83.623e-8342.92PiggyBac transposable element derived 4 OS=Gorilla... [more]
A0A2K6S7R14.133e-8343.40PiggyBac transposable element derived 4 OS=Saimiri... [more]
A0A2K6JV206.500e-8342.92PiggyBac transposable element derived 4 OS=Rhinopi... [more]
F7FDY87.664e-8342.92PiggyBac transposable element derived 4 OS=Callith... [more]
A0A2K5PIR49.035e-8342.92PiggyBac transposable element derived 4 OS=Cebus c... [more]
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BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSAMXT00000043919.11.065e-2024.88pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
ENSAMXT00000014953.22.232e-1823.16pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
si:ch211-130m23.54.389e-1824.18piggyBac transposable element-derived protein 4-li... [more]
ENSAMXT00000057483.17.779e-1823.00piggyBac transposable element-derived protein 4-li... [more]
ENSAMXT00000030524.11.043e-1521.00pep primary_assembly:Astyanax_mexicanus-2.0:8:5821... [more]
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BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 3
Match NameE-valueIdentityDescription
ENSPMAT00000002004.16.023e-2024.12pep scaffold:Pmarinus_7.0:GL490362:1254:2954:1 gen... [more]
ENSPMAT00000007705.18.779e-1727.27pep scaffold:Pmarinus_7.0:GL484572:34:4229:-1 gene... [more]
ENSPMAT00000001816.16.918e-823.83pep scaffold:Pmarinus_7.0:GL492440:267714:269431:-... [more]
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BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
si:ch211-130m23.56.346e-1622.51piggyBac transposable element-derived protein 4-li... [more]
ENSORLT00000021107.21.208e-1020.57piggyBac transposable element-derived protein 4 [S... [more]
ENSORLT00000029842.12.410e-920.31piggyBac transposable element-derived protein 4 [S... [more]
ENSORLT00000039079.12.937e-825.79pep primary_assembly:ASM223467v1:3:33786070:337911... [more]
ENSORLT00000037833.14.806e-821.08piggyBac transposable element-derived protein 4-li... [more]
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BLAST of PiggyBac transposable element-derived protein 4 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000067117.13.802e-2130.39SMESG000067117.1[more]
SMESG000071608.13.718e-1425.42SMESG000071608.1[more]
SMESG000047155.15.774e-1423.15SMESG000047155.1[more]
SMESG000020543.14.533e-826.12SMESG000020543.1[more]
SMESG000056413.11.082e-734.00SMESG000056413.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30019820 ID=SMED30019820|Name=PiggyBac transposable element-derived protein 4|organism=Schmidtea mediterranea sexual|type=transcript|length=2338bp
AGTGGCGGATTTAGATTTTGGTGCGTGAGGGGGGGGGCGAGGAAAATTTT
CCCGGCGCTTGTTTAGAATTGCACCGCATTTTTTAAATAATAAACTTTTA
ATACAAGCTTTTATTATTAAAGATAGCTAACATGACTGTAAAAACATTTA
TCTAACTCGATCATTGGGCAGGTGGCCAGTATTCACGGATATCATGTAAA
ATCTTTTCAAATGGATTTGCACCATTTAATCTTCGTCGCCACATATACTC
ACATAAATAACTATCTAACATGCCACGATGCGTCCCAAAATGACGCTTAT
TTCTTTGTTTCGCTAAACTCCAACATCTTTCAATCATTGATTTTTTGTTA
GGTTGATAATCCATCTTTAAAAGTATTAAATGCCTTATAATAAAATTTAT
TTAAAAATGCGGGGCAATTCTAAACAAGCGCCGTTTATTGTACTGATTTG
ACAGATGGTAGTACTGAACCATGAAATCAATAGAAAATTTTATCAATAAC
ACTGAATAAGTCAATGCGTGACCTGTTAGTTCTTTGTTTTTCTACCACGT
GTTATTTACACCAAAATATGTCATAATCGGAATATAAATAAAAAACCAAA
ACTAACAGCAGTTGAATTAGCAGTGGCTATAGAACATATTGTTTCCAGTT
CTGATGACGGTAATGATATTTCGGATTTGGAAAGTTCGGATGACAGTTGC
AGTGAGATCGAAGATGACGGAACTGTTGTAGATTACGAGAGTCATGCAAC
GGTTGATTATTATCATGATGTTGTTGACGAAGAAACTTTTCATTTGAATA
ATGCACCACAGCAGATTCAACAACAACTGCCTTTTTCCGGCACACCGGGC
ATCAACGTTCATATCTCAATGCCAGGTAACCGTCTTGAATACTTTGGACT
ATTTGTTACTGATCACATTGTTCAATCAATTGTAACGGAAACTAATCGTC
GTCATGGTCTGCGTTTTGCGAATCCATGACTGAAAGCAAGATCAAGATTG
TGATAGTGGTCTATCACAAATCCAGATGAAATAAAAGGTTTCATTGCTAT
AATTTTGTATCAAGGTTTAGTACAAAAACCGCAAACGGCAATGTATTGGA
GTACTAATCTTATGCTAGCCACACCATATATTCGGCGTATTATGACCAAT
GATAACAAAACTCTTTCAGAGGCAGAAACGAATACCGAGAAATCTTTCTA
TAAGATAAAAAAAATTTTTGAAGCGGTTTATTCCAGATTCTCGATAATTT
ATAAATCAGGACAGAACATAGCAATTGACGAATCTCTAATGATTTAGAAA
TGGATATTAGCTAAGAAGCAATATATTCTTCTGAAACGCGCCCGATTTGG
TCTGAAATTGTACTTTCTCTGTGAATCTGGTAGCGGTTATATTTGGAAAG
AATTCACATATAGGACCAGCAATGCATTTGTAACAATCTAGAAACATCGA
AAATCGTCATCACTCTACTGCAAAACCTACCTAGGAAAGTTTATACAGTT
TACATGGACAATTACTACACATCTCCAATGTTGTTTAGAGAACTAATATC
AAATAATACTGATGCAGTTGGGACTGTACGTTTGAATAGAAAGAATATTC
CAGCAGATCCCAAAAAGTAAATAGTTCTTGGTAAATTGTTACACGTTATT
GCGGAAAATAGATGGCCTTGAAGTGTCACGACAAAAAGGATGTTTCAATG
ATGTCAACATTTCACACAGCTGAAGTTCAAGATGTTTTAGCCATCGTGGT
ACAAGAGAAAAGCCTGTGGTCATCTGCGATTACAATGCCAACATGGGTGC
TGTTGATGTTTCGGACCAGATGCTTACATCATACCCAGCTGAAAGAAAGA
GACTCGAAGTTTGGTATAACAGAATTATTCCGTCATCTAATGAACAAAGT
TTCTTTGAACTGTTATTTGTTATTCAAGAAACAAATAGAGAACAAACTTA
TTCATGTTCAGTTTCGATTGAAACTCATTGAGTGGCTCATTGATGTTTAC
CATATGCCAGAGCATCAAGCCGATACTGGGCACACGGCAACTGCTGGAGG
TGACCCTTTTCGCTTGACCGGGTGCCATTTTCCAACCTATGGACTTGTCA
CAGCTAAGAAAATGAATGCACAGAGAATTTGTGCAGTTTGTTCTTCGAAA
GAAGTGGATGGTAAGAAAATCCGTAGAGTCATGATATGAGTCTGCTGACT
GTGATGTTCGTTTGTGTGCAGCACCTTGCTTTCAAATCTATCACATTATT
CGAAAATTCTGATGTCAATCTGATGTAAATTTGAGTATTGACAAAATTTT
GATAATTAAAAAACTTATTTCATGCCTTTTTATATCAC
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protein sequence of SMED30019820-orf-1

>SMED30019820-orf-1 ID=SMED30019820-orf-1|Name=SMED30019820-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=176bp
MALKCHDKKDVSMMSTFHTAEVQDVLAIVVQEKSLWSSAITMPTWVLLMF
RTRCLHHTQLKERDSKFGITELFRHLMNKVSLNCYLLFKKQIENKLIHVQ
FRLKLIEWLIDVYHMPEHQADTGHTATAGGDPFRLTGCHFPTYGLVTAKK
MNAQRICAVCSSKEVDGKKIRRVMI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: INTERPRO
TermDefinition
IPR029526PGBD
Vocabulary: cellular component
TermDefinition
GO:0005622intracellular
Vocabulary: biological process
TermDefinition
GO:0006915apoptotic process