SMED30019814
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30019814 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 2
Alignments
SMED30019814 aligns in the following genomic locations:
Homology
BLAST of SMED30019814 vs. Planmine SMEST
Match: SMESG000050492.1 (SMESG000050492.1) HSP 1 Score: 871.692 bits (2251), Expect = 0.000e+0 Identity = 458/463 (98.92%), Postives = 459/463 (99.14%), Query Frame = 1 Query: 28 TRDTNLIAMLFQLSTNLYLLTSILIGFIKCQPNNYIHAEMEGNLDDKRGFNFTLVTRNSSGVILYAVNSERNFLIKLENSRIIMKYKNYTIEKPLWKICGFSVNHFWKYFVMLRHLEVQVTCDGKFHSLVFKDIKKVIYSNYXXXXXXXXXXXXXYIWMGNVGPYTGEGDVVTQPMKNQLYGCLDKFYFHYKLIQMFTKASNFKVCSDNFYNETICYNETSCQKKVTSYKIDDCSLLSMNGIYCLPNYQIYGNLVNSFQRSDQLKKIVNFKLYWKSLLENNFCLLEIKSRDSTIVIFINQNSQMKMKLPQRNGILLRNLSIKNKRNIQQFLLVSVTPSELWVKVYSDGDIKVFVTSIKFDPITINVGKSQQSEICRTFQGMIDVQYDPTQFTLDVGILKKPLFISGIXXXXXXXXXXXXXXXXXTIRFSSMSECAWKSHLKFKKTDKTFNREDSDKYSTIYDS 1416 TRDTNLIAMLF LSTNLYLLTSILIGFIKCQPNNYIHAEMEGNLDDKRGFNFTLVTRNSSGVILYAV+SERNFLIKLENSRIIMKYKNYTIEKPLWKICGFSVNH WKYFVMLRHLEVQVTCDGKFHSLVFKDIKKVIYSNYPKPKELKESSSKSYIWMGNVGPYTGEGDVVTQPMKNQLYGCLDKFYFHYKLIQMFTKASNFKVCSDNFYNETICYNETSCQKKVTSYKIDDCSLLSMNGIYCLPNYQ YGNLVNSFQRSDQLKKIVNFKLYWKSLLENNFCLLEIKSRDSTIVIFINQNSQMKMKLPQRNGILLRNLSIKNKRNIQQFLLVSVTPSELWVKVYSDGDIKVFVTSIKFDPITINVGKSQQSEICRTFQGMIDVQYDPTQFTLDVGILKKPLFISGILLVLFVGLLVVY LKVKTIRFSSMSECAWKSHLKFKKTDKTFNREDSDKYSTIYDS Sbjct: 3 TRDTNLIAMLFHLSTNLYLLTSILIGFIKCQPNNYIHAEMEGNLDDKRGFNFTLVTRNSSGVILYAVDSERNFLIKLENSRIIMKYKNYTIEKPLWKICGFSVNHIWKYFVMLRHLEVQVTCDGKFHSLVFKDIKKVIYSNYPKPKELKESSSKSYIWMGNVGPYTGEGDVVTQPMKNQLYGCLDKFYFHYKLIQMFTKASNFKVCSDNFYNETICYNETSCQKKVTSYKIDDCSLLSMNGIYCLPNYQTYGNLVNSFQRSDQLKKIVNFKLYWKSLLENNFCLLEIKSRDSTIVIFINQNSQMKMKLPQRNGILLRNLSIKNKRNIQQFLLVSVTPSELWVKVYSDGDIKVFVTSIKFDPITINVGKSQQSEICRTFQGMIDVQYDPTQFTLDVGILKKPLFISGILLVLFVGLLVVYSLKVKTIRFSSMSECAWKSHLKFKKTDKTFNREDSDKYSTIYDS 465
BLAST of SMED30019814 vs. Planmine SMEST
Match: SMESG000050492.1 (SMESG000050492.1) HSP 1 Score: 855.514 bits (2209), Expect = 0.000e+0 Identity = 450/455 (98.90%), Postives = 451/455 (99.12%), Query Frame = 1 Query: 52 MLFQLSTNLYLLTSILIGFIKCQPNNYIHAEMEGNLDDKRGFNFTLVTRNSSGVILYAVNSERNFLIKLENSRIIMKYKNYTIEKPLWKICGFSVNHFWKYFVMLRHLEVQVTCDGKFHSLVFKDIKKVIYSNYXXXXXXXXXXXXXYIWMGNVGPYTGEGDVVTQPMKNQLYGCLDKFYFHYKLIQMFTKASNFKVCSDNFYNETICYNETSCQKKVTSYKIDDCSLLSMNGIYCLPNYQIYGNLVNSFQRSDQLKKIVNFKLYWKSLLENNFCLLEIKSRDSTIVIFINQNSQMKMKLPQRNGILLRNLSIKNKRNIQQFLLVSVTPSELWVKVYSDGDIKVFVTSIKFDPITINVGKSQQSEICRTFQGMIDVQYDPTQFTLDVGILKKPLFISGIXXXXXXXXXXXXXXXXXTIRFSSMSECAWKSHLKFKKTDKTFNREDSDKYSTIYDS 1416 MLF LSTNLYLLTSILIGFIKCQPNNYIHAEMEGNLDDKRGFNFTLVTRNSSGVILYAV+SERNFLIKLENSRIIMKYKNYTIEKPLWKICGFSVNH WKYFVMLRHLEVQVTCDGKFHSLVFKDIKKVIYSNYPKPKELKESSSKSYIWMGNVGPYTGEGDVVTQPMKNQLYGCLDKFYFHYKLIQMFTKASNFKVCSDNFYNETICYNETSCQKKVTSYKIDDCSLLSMNGIYCLPNYQ YGNLVNSFQRSDQLKKIVNFKLYWKSLLENNFCLLEIKSRDSTIVIFINQNSQMKMKLPQRNGILLRNLSIKNKRNIQQFLLVSVTPSELWVKVYSDGDIKVFVTSIKFDPITINVGKSQQSEICRTFQGMIDVQYDPTQFTLDVGILKKPLFISGILLVLFVGLLVVY LKVKTIRFSSMSECAWKSHLKFKKTDKTFNREDSDKYSTIYDS Sbjct: 1 MLFHLSTNLYLLTSILIGFIKCQPNNYIHAEMEGNLDDKRGFNFTLVTRNSSGVILYAVDSERNFLIKLENSRIIMKYKNYTIEKPLWKICGFSVNHIWKYFVMLRHLEVQVTCDGKFHSLVFKDIKKVIYSNYPKPKELKESSSKSYIWMGNVGPYTGEGDVVTQPMKNQLYGCLDKFYFHYKLIQMFTKASNFKVCSDNFYNETICYNETSCQKKVTSYKIDDCSLLSMNGIYCLPNYQTYGNLVNSFQRSDQLKKIVNFKLYWKSLLENNFCLLEIKSRDSTIVIFINQNSQMKMKLPQRNGILLRNLSIKNKRNIQQFLLVSVTPSELWVKVYSDGDIKVFVTSIKFDPITINVGKSQQSEICRTFQGMIDVQYDPTQFTLDVGILKKPLFISGILLVLFVGLLVVYSLKVKTIRFSSMSECAWKSHLKFKKTDKTFNREDSDKYSTIYDS 455
BLAST of SMED30019814 vs. Planmine SMEST
Match: SMESG000050280.1 (SMESG000050280.1) HSP 1 Score: 191.045 bits (484), Expect = 4.167e-55 Identity = 130/389 (33.42%), Postives = 209/389 (53.73%), Query Frame = 1 Query: 79 YLLTSILIGFIKCQPNNYIHAEMEGNLDDKRGFNFTLVTRNSSGVILYAVNSERN-FLIKLE---NSRI-IMKYKNYTIEKPLWKICG--FSVNHFWKYFVMLRHLEVQVTCDGKFHSLVFKDIK-KVIYSNYXXXXXXXXXXXXXYIWMGNVGPYTGEGDVVTQPMKNQLYGCLDKFYFHYKLIQMFTKASNFKVCSDNFYNETICYNETSCQKKVTSYKIDDCSLLSMNGIYCLPNYQIYGNLVNSFQRSDQLKKIVNFKLYWKSLLENNFCLLEIK-SRDSTIVIFINQNSQMKMKLPQRNGILL-RNLSIKNKRNIQQFLLVSVTPSELWVKVYSDGDIKVFVTSIKFDPITINVGKSQQSEICRTFQGMIDVQYDPTQFTLDVG 1215 +LLT+ LI FI NY ++E +G+L ++ FNF+L SG+ LYAV+S N FLI++E N +I I+KY L +CG F + + V LR LEV C G + +++ ++ YP P++ +++K Y W+GN+G Y G G ++ P+ NQL GCLDKFYF+ + + K N +NE++C NETSC SY I+DC+L S+N YC +Y + ++ + K + +L WKS NFCL++IK ++ I +++N+ +Q+ + I++ RNL+ K K L++ ++ ++ S +I F+ P+ + +G IC+TF+G ID+ YDPTQ +L G Sbjct: 4 FLLTNFLISFISA---NYTYSESQGDL--QKEFNFSLFIETFSGITLYAVDSNNNKFLIEMELEENLQIAIIKYNKLFNRVKLENLCGCRFCSHASMAFVVRLRRLEVNAKCKGVDKLIRLENVNGDGQFNGYPLPRKRSNNTNKIYFWIGNIGYYFGLGKILYVPLNNQLTGCLDKFYFNNRFLTFEQKGKMMLNSCPNVFNESVC-NETSCMADQISYGINDCALASVNPRYCRKALPLYSKSKDLYEIKIRAKFPITIRLEWKSFYLKNFCLIDIKMGKNKFITMYLNKMNQISFAISHNRSIVVERNLT-KMKELDNNLLIIIFNVTQFMIRAASANEIYTFIEPFNERPMEMRIGSGGNINICQTFEGAIDIFYDPTQISLRTG 385
BLAST of SMED30019814 vs. Planmine SMEST
Match: SMESG000050280.1 (SMESG000050280.1) HSP 1 Score: 191.045 bits (484), Expect = 4.445e-55 Identity = 130/389 (33.42%), Postives = 209/389 (53.73%), Query Frame = 1 Query: 79 YLLTSILIGFIKCQPNNYIHAEMEGNLDDKRGFNFTLVTRNSSGVILYAVNSERN-FLIKLE---NSRI-IMKYKNYTIEKPLWKICG--FSVNHFWKYFVMLRHLEVQVTCDGKFHSLVFKDIK-KVIYSNYXXXXXXXXXXXXXYIWMGNVGPYTGEGDVVTQPMKNQLYGCLDKFYFHYKLIQMFTKASNFKVCSDNFYNETICYNETSCQKKVTSYKIDDCSLLSMNGIYCLPNYQIYGNLVNSFQRSDQLKKIVNFKLYWKSLLENNFCLLEIK-SRDSTIVIFINQNSQMKMKLPQRNGILL-RNLSIKNKRNIQQFLLVSVTPSELWVKVYSDGDIKVFVTSIKFDPITINVGKSQQSEICRTFQGMIDVQYDPTQFTLDVG 1215 +LLT+ LI FI NY ++E +G+L ++ FNF+L SG+ LYAV+S N FLI++E N +I I+KY L +CG F + + V LR LEV C G + +++ ++ YP P++ +++K Y W+GN+G Y G G ++ P+ NQL GCLDKFYF+ + + K N +NE++C NETSC SY I+DC+L S+N YC +Y + ++ + K + +L WKS NFCL++IK ++ I +++N+ +Q+ + I++ RNL+ K K L++ ++ ++ S +I F+ P+ + +G IC+TF+G ID+ YDPTQ +L G Sbjct: 7 FLLTNFLISFISA---NYTYSESQGDL--QKEFNFSLFIETFSGITLYAVDSNNNKFLIEMELEENLQIAIIKYNKLFNRVKLENLCGCRFCSHASMAFVVRLRRLEVNAKCKGVDKLIRLENVNGDGQFNGYPLPRKRSNNTNKIYFWIGNIGYYFGLGKILYVPLNNQLTGCLDKFYFNNRFLTFEQKGKMMLNSCPNVFNESVC-NETSCMADQISYGINDCALASVNPRYCRKALPLYSKSKDLYEIKIRAKFPITIRLEWKSFYLKNFCLIDIKMGKNKFITMYLNKMNQISFAISHNRSIVVERNLT-KMKELDNNLLIIIFNVTQFMIRAASANEIYTFIEPFNERPMEMRIGSGGNINICQTFEGAIDIFYDPTQISLRTG 388
BLAST of SMED30019814 vs. Planmine SMEST
Match: SMESG000050503.1 (SMESG000050503.1) HSP 1 Score: 171.014 bits (432), Expect = 1.192e-47 Identity = 114/374 (30.48%), Postives = 201/374 (53.74%), Query Frame = 1 Query: 121 PNNYIHAEMEGNLDDKRGFNFTLVTRNSSGVILYAVNSERNFLIKLENSR-IIMKYKNYTIEKPLWKICGFSVNH-------FWKYFVMLRHLEVQVTCDGKFHSLVFKDIKKV-IYSNYXXXXXXXXXXXXXYIWMGNVGPYTGEGDVVTQPMKNQLYGCLDKFYFHYKLIQMFTKASNFKVCSDNFYNETICYNETSCQKKVTSYKIDDCSLLSMNGIYCLP---NYQIYGNLVNSFQRSDQLKKIVNFKLYWKSLLENNFCLLEIKSRDSTIVIF-INQNSQMKMKLPQRNGILLRNLSIKNKRNIQQFLLVSVTPSELWVKVYSDGDIKVFVTSIKFDPITINVGKSQQSEICRTFQGMIDVQYDPTQFT 1203 P +I ++ GNL K F F+LVT N +G+I Y +S FLIKL N +I+KY+ + +++ + +IC H +W++ V+L ++V + C+ + + ++ ++ I S YP PK +K S KS++WMGN+ + E + P+K+ L GC+DKF+++ K IQ ++ F N Y E C N +C K SY IDDCS ++N +C P N Y + + +++ I+ KL W +L+ + FCL++ K+R+ ++++F +NQN ++ + + G++ + L +K + LLV T L VK++ + I ++ DP +I G IC F G +++ Y+ +Q + Sbjct: 21 PAGFISTQLNGNLAQKNRFQFSLVTENLNGIIFYGFDSTDQFLIKLINGENLIIKYQEFLVKRFVKEICTVDPYHSKYPKYFYWEFLVLLEQMKVIIKCNRRENVVILSGPRQYGILSAYPNPKVVKISKMKSFVWMGNIYVNSTEESLCFSPLKDTLIGCMDKFFYNNKQIQFQIYSNKFLPECKNQYREIEC-NHVTCTNKSISYAIDDCSFSALNRRFCWPDDANLPYYSKPKHFYVVNNKPNLIL--KLKWHNLITDIFCLMQFKTRNKSLIVFSLNQNHKIVLDIRTSGGLIKKVLPLKAIKESLNVLLVEFTNRGLIVKIFINDSIMRYIEPFHEDPESIVTGTCDVDNICSQFIGKMELIYNHSQIS 391 The following BLAST results are available for this feature:
BLAST of SMED30019814 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30019814 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30019814 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30019814 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30019814 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30019814 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30019814 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30019814 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30019814 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30019814 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30019814 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30019814 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30019814 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30019814 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30019814 ID=SMED30019814|Name=SMED30019814|organism=Schmidtea mediterranea sexual|type=transcript|length=1420bpback to top protein sequence of SMED30019814-orf-1 >SMED30019814-orf-1 ID=SMED30019814-orf-1|Name=SMED30019814-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=456bp MLFQLSTNLYLLTSILIGFIKCQPNNYIHAEMEGNLDDKRGFNFTLVTRNback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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