SMED30019787

Overview
NameSMED30019787
Smed IDSMED30019787
Length (bp)2479
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30019787 (SMED30019787) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30019787 (SMED30019787) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30019787 (SMED30019787) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30019787 (SMED30019787) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30019787

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
epidermisSMED30019787h1SMcG0017704 dd_Smed_v4_12185_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of SMED30019787 vs. Planmine SMEST
Match: SMESG000048292.1 (SMESG000048292.1)

HSP 1 Score: 1325.46 bits (3429), Expect = 0.000e+0
Identity = 752/755 (99.60%), Postives = 753/755 (99.74%), Query Frame = 2
Query:   20 MMDLNNDLVSLLITYIXXXXXXXXXXXXXXXNCELKLCLSVSHSNCGWNDCGTDLIMIHTRLNQSNEFIEENIDQVPNQFYIKSQIVSHCTGMLITDIGGANKIMSIETIVTKHNYNCETVFKTFVTRLPDGKKLKTQRSFTREISNYSDIKFKLRNQFSVVISDDEPVIQKPDVARPPTKIKVKKRPSFKAFTXXXXXXXNDKQDAPRHKSGAHPEEEHKVLQPMXXXXXXXXXXXXXXXXXRTVSIEDKLSIIPPTGRGEGDSKQSVMQEVKTFSTEKNEMEKEKPTNILAKISLKNGTKIFEIINDNIPFIXXXXXXXXXXXXXXXXXXXXXXXGSFLHFDFAVSSDIRNTINRIIKDMSCDCEKKKFNMISDSLTYIAKKDQESKKQIWLEIKRFDNTKKHFIDCAVWICNKKNANYQRKSLPPNEDFHDLCRICHDIDQHFNENLCTIFVPFLKEIETKERQELCLCERXXXXXXXSFIYSTMTSVKINDNFRDSFNKVTNHFMDCVCNWISPAINPSIVDTNNNLDDKIETTSCNCYKRRMQILAHFIGHIFFKKALRTDDGNEFGLKTKHSTSCPRYLSTSGTSPIAYTPSPFSNGYPVFESFSPTISPTQGXXXXXXXXXXXXXXXXFSSMHSIKSLNDVIQSIKNGYEIECSIETIEYFIKCKDYDSFDKGIFHVTEENVSIENMCLFFYLIRKIVRYIDDPSCEDNEDIKRIIEYSEXXXXXXXXDEISKQGGLEKFILGIDSLD 2284
            MMDLNNDLVSLLITYIEQESEEEQRFIEDQENCELKLCLSVSHSNCGWNDCGTDLIMIHTRLNQSNEFIEENIDQVPNQFYIKSQIVSHCTGMLITDIGGANKIMSIETIVTKHNYNCETVFKTFVTRLPDGKKLKTQRSFTREISNYSDIKFKLRNQFSVVISDDEPVIQKPDVARPPTKIKVKKRPSFKAFTKSKKSKSNDKQDAPRHKSGAHPEEEHKVLQPMKKQKSSIFKSFKN+FKSRTVSIEDKLSIIPPTGRGEGDSKQSVMQEVKTFSTEKNEMEKEKPTNILAKISLKNGTKIFEIINDNIPFIDDVEDEESEEEECVKSSKSDSDCGSFLHFDFAVSSDIRNTINRIIKDMSCDCEKKKFNMISDSLTYIAKKDQESKKQI LEIKRFDNTKKHFIDCAVWICNKKNANYQRKSLPPNEDFHDLCRICHDIDQHFNENLCTIFVPFLKEIETKERQELCLCERKKKEKIKSFIYSTMTSVKINDNFRDSFNKVTNHFMDCVCNWISPAINPS VDTNNNLDDKIETTSCNCYKRRMQILAHFIGHIFFKKALRTDDGNEFGLKTKHSTSCPRYLSTSGTSPIAYTPSPFSNGYPVFESFSPTISPTQGKKHSSESKRKKSRTNSFSSMHSIKSLNDVIQSIKNGYEIECSIETIEYFIKCKDYDSFDKGIFHVTEENVSIENMCLFFYLIRKIVRYIDDPSCEDNEDIKRIIEYSEKYLLKYYLDEISKQGGLEKFILGIDSLD
Sbjct:    1 MMDLNNDLVSLLITYIEQESEEEQRFIEDQENCELKLCLSVSHSNCGWNDCGTDLIMIHTRLNQSNEFIEENIDQVPNQFYIKSQIVSHCTGMLITDIGGANKIMSIETIVTKHNYNCETVFKTFVTRLPDGKKLKTQRSFTREISNYSDIKFKLRNQFSVVISDDEPVIQKPDVARPPTKIKVKKRPSFKAFTKSKKSKSNDKQDAPRHKSGAHPEEEHKVLQPMKKQKSSIFKSFKNLFKSRTVSIEDKLSIIPPTGRGEGDSKQSVMQEVKTFSTEKNEMEKEKPTNILAKISLKNGTKIFEIINDNIPFIDDVEDEESEEEECVKSSKSDSDCGSFLHFDFAVSSDIRNTINRIIKDMSCDCEKKKFNMISDSLTYIAKKDQESKKQIRLEIKRFDNTKKHFIDCAVWICNKKNANYQRKSLPPNEDFHDLCRICHDIDQHFNENLCTIFVPFLKEIETKERQELCLCERKKKEKIKSFIYSTMTSVKINDNFRDSFNKVTNHFMDCVCNWISPAINPSTVDTNNNLDDKIETTSCNCYKRRMQILAHFIGHIFFKKALRTDDGNEFGLKTKHSTSCPRYLSTSGTSPIAYTPSPFSNGYPVFESFSPTISPTQGKKHSSESKRKKSRTNSFSSMHSIKSLNDVIQSIKNGYEIECSIETIEYFIKCKDYDSFDKGIFHVTEENVSIENMCLFFYLIRKIVRYIDDPSCEDNEDIKRIIEYSEKYLLKYYLDEISKQGGLEKFILGIDSLD 755          
BLAST of SMED30019787 vs. Planmine SMEST
Match: SMESG000048292.1 (SMESG000048292.1)

HSP 1 Score: 1322.76 bits (3422), Expect = 0.000e+0
Identity = 751/754 (99.60%), Postives = 752/754 (99.73%), Query Frame = 2
Query:   23 MDLNNDLVSLLITYIXXXXXXXXXXXXXXXNCELKLCLSVSHSNCGWNDCGTDLIMIHTRLNQSNEFIEENIDQVPNQFYIKSQIVSHCTGMLITDIGGANKIMSIETIVTKHNYNCETVFKTFVTRLPDGKKLKTQRSFTREISNYSDIKFKLRNQFSVVISDDEPVIQKPDVARPPTKIKVKKRPSFKAFTXXXXXXXNDKQDAPRHKSGAHPEEEHKVLQPMXXXXXXXXXXXXXXXXXRTVSIEDKLSIIPPTGRGEGDSKQSVMQEVKTFSTEKNEMEKEKPTNILAKISLKNGTKIFEIINDNIPFIXXXXXXXXXXXXXXXXXXXXXXXGSFLHFDFAVSSDIRNTINRIIKDMSCDCEKKKFNMISDSLTYIAKKDQESKKQIWLEIKRFDNTKKHFIDCAVWICNKKNANYQRKSLPPNEDFHDLCRICHDIDQHFNENLCTIFVPFLKEIETKERQELCLCERXXXXXXXSFIYSTMTSVKINDNFRDSFNKVTNHFMDCVCNWISPAINPSIVDTNNNLDDKIETTSCNCYKRRMQILAHFIGHIFFKKALRTDDGNEFGLKTKHSTSCPRYLSTSGTSPIAYTPSPFSNGYPVFESFSPTISPTQGXXXXXXXXXXXXXXXXFSSMHSIKSLNDVIQSIKNGYEIECSIETIEYFIKCKDYDSFDKGIFHVTEENVSIENMCLFFYLIRKIVRYIDDPSCEDNEDIKRIIEYSEXXXXXXXXDEISKQGGLEKFILGIDSLD 2284
            MDLNNDLVSLLITYIEQESEEEQRFIEDQENCELKLCLSVSHSNCGWNDCGTDLIMIHTRLNQSNEFIEENIDQVPNQFYIKSQIVSHCTGMLITDIGGANKIMSIETIVTKHNYNCETVFKTFVTRLPDGKKLKTQRSFTREISNYSDIKFKLRNQFSVVISDDEPVIQKPDVARPPTKIKVKKRPSFKAFTKSKKSKSNDKQDAPRHKSGAHPEEEHKVLQPMKKQKSSIFKSFKN+FKSRTVSIEDKLSIIPPTGRGEGDSKQSVMQEVKTFSTEKNEMEKEKPTNILAKISLKNGTKIFEIINDNIPFIDDVEDEESEEEECVKSSKSDSDCGSFLHFDFAVSSDIRNTINRIIKDMSCDCEKKKFNMISDSLTYIAKKDQESKKQI LEIKRFDNTKKHFIDCAVWICNKKNANYQRKSLPPNEDFHDLCRICHDIDQHFNENLCTIFVPFLKEIETKERQELCLCERKKKEKIKSFIYSTMTSVKINDNFRDSFNKVTNHFMDCVCNWISPAINPS VDTNNNLDDKIETTSCNCYKRRMQILAHFIGHIFFKKALRTDDGNEFGLKTKHSTSCPRYLSTSGTSPIAYTPSPFSNGYPVFESFSPTISPTQGKKHSSESKRKKSRTNSFSSMHSIKSLNDVIQSIKNGYEIECSIETIEYFIKCKDYDSFDKGIFHVTEENVSIENMCLFFYLIRKIVRYIDDPSCEDNEDIKRIIEYSEKYLLKYYLDEISKQGGLEKFILGIDSLD
Sbjct:    1 MDLNNDLVSLLITYIEQESEEEQRFIEDQENCELKLCLSVSHSNCGWNDCGTDLIMIHTRLNQSNEFIEENIDQVPNQFYIKSQIVSHCTGMLITDIGGANKIMSIETIVTKHNYNCETVFKTFVTRLPDGKKLKTQRSFTREISNYSDIKFKLRNQFSVVISDDEPVIQKPDVARPPTKIKVKKRPSFKAFTKSKKSKSNDKQDAPRHKSGAHPEEEHKVLQPMKKQKSSIFKSFKNLFKSRTVSIEDKLSIIPPTGRGEGDSKQSVMQEVKTFSTEKNEMEKEKPTNILAKISLKNGTKIFEIINDNIPFIDDVEDEESEEEECVKSSKSDSDCGSFLHFDFAVSSDIRNTINRIIKDMSCDCEKKKFNMISDSLTYIAKKDQESKKQIRLEIKRFDNTKKHFIDCAVWICNKKNANYQRKSLPPNEDFHDLCRICHDIDQHFNENLCTIFVPFLKEIETKERQELCLCERKKKEKIKSFIYSTMTSVKINDNFRDSFNKVTNHFMDCVCNWISPAINPSTVDTNNNLDDKIETTSCNCYKRRMQILAHFIGHIFFKKALRTDDGNEFGLKTKHSTSCPRYLSTSGTSPIAYTPSPFSNGYPVFESFSPTISPTQGKKHSSESKRKKSRTNSFSSMHSIKSLNDVIQSIKNGYEIECSIETIEYFIKCKDYDSFDKGIFHVTEENVSIENMCLFFYLIRKIVRYIDDPSCEDNEDIKRIIEYSEKYLLKYYLDEISKQGGLEKFILGIDSLD 754          
BLAST of SMED30019787 vs. Planmine SMEST
Match: SMESG000048297.1 (SMESG000048297.1)

HSP 1 Score: 151.754 bits (382), Expect = 1.171e-38
Identity = 132/419 (31.50%), Postives = 214/419 (51.07%), Query Frame = 2
Query:   38 DLVSLLITYIXXXXXXXXXXXXXXXNCELKLCLSVSHSNCGWNDCGTDLIMIHTRLNQSNEFIEENIDQVPNQFYIKSQIVSHCTGMLITDIGGANKIMSIETIVTKHNYNCETVFKTFVTRLPDGKKLKTQRSFTREISN--YSDIKFKLRNQFSVVISDDEPVIQKPDVARPPTKIKVKKRPSFKAFTXXXXXXXNDKQDAPRHKSGAHPEEEHKVLQPMXXXXXXXXXXXXXXXXXRTVSIEDKLSIIPPTGRGEGDSKQSVMQEVKTFSTEKNEMEKEKPTNILAKISLKNGTKIFEIIN---DNIPFIXXXXXXXXXXXXXXXXXXXXXXXGSFLHFDFAVSSDIRNTINRIIKDMSCDCEKKKFNMISDSLTYIAKKDQESKKQIWLEIKRFDNTKKHFIDCAVWICNKKNAN 1279
            DL   L  Y+ +ES +E +FI +Q+ C+L+LC++V+HSNC   + G DL+MI+T+L      I+ N ++  N FYIKSQ VSHC G+   +  G +K+MS++T++T+ N  CETVF T VT+  +GK+L + RSFTREI +  ++D    + + F VV SD+E    K   ++     K  K+P  K+    K SK    +     K   +P     V++P+   +                    KLS+             + MQ+   FSTE N    +KP NILAKIS+ N T  FE+I+   D+ PF+D+  + +   E      ++     S+L F+ +  ++       + KD +C+CEK++ +++ + + +  +   E K      +  F+   +HF DC       KN+N
Sbjct:    7 DLRVFLEYYVRRESNKEHKFIVEQKKCQLQLCVTVNHSNCPSTNPGKDLLMINTKL------IQLNHEEKENSFYIKSQKVSHCIGLTGHNSDGDHKVMSVQTVITRANSECETVFMTSVTKAQNGKRLTSHRSFTREIVDCQHNDDCINIHDNFEVVFSDNENSPGKKTNSKKNIVDKTFKKPFLKSIKLPKLSKKKSLEKRDELK---YP----TVIKPILGNR--------------------KLSL-------------ACMQD---FSTEFNPTMNQKPRNILAKISVSNKTHDFEVIDEVIDDTPFMDEENENDINNESAPNLQEASPTSDSYLQFNLSTCNNSNTETADLFKDTTCNCEKRRISVLVEFVEWKLRHRSEFKND---SLNTFNEQTRHFSDCHKLSSGIKNSN 373          

HSP 2 Score: 94.7449 bits (234), Expect = 6.232e-20
Identity = 67/228 (29.39%), Postives = 119/228 (52.19%), Query Frame = 2
Query: 1595 DTNNNLDDKIETTSCNCYKRRMQILAHFI-GHIFFKKALRTDDGNEFGLKTKHSTSCPRYLSTSGTSPIAYTPSPFSNGYPVFESFSPTISPTQGXXXXXXXXXXXXXXXXFSSMHSIKSLNDVIQSIKNGYEIECSIETIEYFIKCKDYDSFDKGIFHVTEENVSIENMCLFFYLIRKIVRYIDDPSCEDNEDIKRIIEYSEXXXXXXXXDEISKQGGLEKFILGID 2275
            ++N    D  + T+CNC KRR+ +L  F+   +  +   + D  N F  +T+H + C +  S    S      S   N     +SF+       GK      +    R+ + S M S+ +L+ ++ ++K   +I+CSI+ + YF     Y  F+K +  +  +N S+EN  L  ++IR I+++ DDP+ +DNE I++I+ +S ++L+K+Y   I   GG++KFI   D
Sbjct:  306 NSNTETADLFKDTTCNCEKRRISVLVEFVEWKLRHRSEFKNDSLNTFNEQTRHFSDCHKLSSGIKNSNSKLFFSSSQNSIFHSDSFN-------GKDQLLTPEFNLERSRNNSMMESVNNLDKILDNMKRINKIQCSIKHVNYFKSLTKYQDFEKQLLFLIGQNDSVENFSLILFMIRSIIQHADDPNTKDNEIIQKIMNFSTEFLIKFYGKLIKDLGGIDKFIRNDD 526          
BLAST of SMED30019787 vs. Planmine SMEST
Match: SMESG000048287.1 (SMESG000048287.1)

HSP 1 Score: 107.071 bits (266), Expect = 7.842e-24
Identity = 76/216 (35.19%), Postives = 117/216 (54.17%), Query Frame = 2
Query: 1637 CNCYKRRMQILAHFIGHIFFKKALRTDDGNEFGLKTKHSTSCPRYLSTSGTSPIAYTPSPFSNGYPVFESFSPTISPTQGXXXXXXXXXXXXXXXXFSSMHSIKSLNDVIQSIKNGYEIECSIETIEYFIKCKDYDSFDKGIFHVTEENVSIENMCLFFYLIRKIVRYIDDPSCEDNEDIKRIIEYSEXXXXXXXXDEISKQGGLEKFILGIDSLD 2284
            C C +R  +I+  ++      +     +  ++   ++H T CP Y   S +SP   T    S+         P  +  +                   S++S   L+ + ++IK   EI C  ETI YFI+ KDY++++KG+  V +  VS+ENM LFF LIR IV+ IDDP+  +N+DIKRI+ +SE +L+KYYL EISK G L++FIL  +S+D
Sbjct:  363 CKCKERTKEIMIKYLLSKLENRKFDVQETEKYDNVSQHFTDCPSY---STSSPDRRTIHKKSSTSSPNSLSVPIGNRNRRGSVI--------------SLNSESHLDVIFENIKFLNEIHCPAETISYFIESKDYETYEKGLLKVLDSKVSLENMILFFNLIRIIVKQIDDPTYGENKDIKRIMSFSEGFLIKYYLAEISKHGELDQFILHSESVD 561          

HSP 2 Score: 106.301 bits (264), Expect = 1.384e-23
Identity = 74/145 (51.03%), Postives = 96/145 (66.21%), Query Frame = 2
Query:   20 MMDLNNDLVSLLITYIXXXXXXXXXXXXXXXNCELKLCLSVSHSNCGWNDCGTDLIMIHTRLNQSNEFIEENIDQVPNQFYIKSQIVSHCTGMLITDIGGANKIMSIETIVTKHNYNCETVFKTFVTRLPDGKKLKTQRSFTREI 454
            M  L  D+ SLL  Y+E+ES+EE+R IEDQ+ CELK+CLSV  S     +   + I IHT+LNQ+NE I++  D   N FYIKSQ V+H T M         +I+SIET+VTK  YNCE+VFKT + R  +GK+ +TQRSF R I
Sbjct:    1 MSKLEGDIKSLLKYYVERESDEEKRLIEDQKKCELKVCLSVHISRKDDVNEDNNPITIHTKLNQANE-IQKERDSDENHFYIKSQTVTHQTRMSSVHQYYDGQIISIETVVTKPRYNCESVFKTLIHRTKNGKRDQTQRSFARNI 144          

HSP 3 Score: 60.077 bits (144), Expect = 5.262e-9
Identity = 49/147 (33.33%), Postives = 81/147 (55.10%), Query Frame = 2
Query:  815 KQSVMQEVKTFSTEKNEMEKEKPTNILAKISLKNGTKIFEIINDNIPFIXXXXXXXXXXXXXXXXXXXXXXXGSFLHFDFAVSSDIRNTINRIIKDMSCDCEKKKFNMISDSLTYIAKKDQESKKQIWLEIKRFDNTKKHFIDCAVW 1255
            K+S+++  K  STE++EMEKEKPTNI+A+IS    TK FEII D I FID+ ++E     +   + +      SF  F+F    + +     I+    C C+++   ++   + Y+  K  E++K    E +++DN  +HF DC  +
Sbjct:  268 KKSIIELKKYISTEESEMEKEKPTNIIARISTNAKTKAFEIIKDEIMFIDEEDEENDLNFDDSSNKRIPLSE-SFRSFNFLCHQESKP--KEIMAVEVCKCKERTKEIM---IKYLLSK-LENRKFDVQETEKYDNVSQHFTDCPSY 407          
BLAST of SMED30019787 vs. Planmine SMEST
Match: SMESG000048297.1 (SMESG000048297.1)

HSP 1 Score: 94.7449 bits (234), Expect = 2.876e-20
Identity = 67/228 (29.39%), Postives = 119/228 (52.19%), Query Frame = 2
Query: 1595 DTNNNLDDKIETTSCNCYKRRMQILAHFI-GHIFFKKALRTDDGNEFGLKTKHSTSCPRYLSTSGTSPIAYTPSPFSNGYPVFESFSPTISPTQGXXXXXXXXXXXXXXXXFSSMHSIKSLNDVIQSIKNGYEIECSIETIEYFIKCKDYDSFDKGIFHVTEENVSIENMCLFFYLIRKIVRYIDDPSCEDNEDIKRIIEYSEXXXXXXXXDEISKQGGLEKFILGID 2275
            ++N    D  + T+CNC KRR+ +L  F+   +  +   + D  N F  +T+H + C +  S    S      S   N     +SF+       GK      +    R+ + S M S+ +L+ ++ ++K   +I+CSI+ + YF     Y  F+K +  +  +N S+EN  L  ++IR I+++ DDP+ +DNE I++I+ +S ++L+K+Y   I   GG++KFI   D
Sbjct:  208 NSNTETADLFKDTTCNCEKRRISVLVEFVEWKLRHRSEFKNDSLNTFNEQTRHFSDCHKLSSGIKNSNSKLFFSSSQNSIFHSDSFN-------GKDQLLTPEFNLERSRNNSMMESVNNLDKILDNMKRINKIQCSIKHVNYFKSLTKYQDFEKQLLFLIGQNDSVENFSLILFMIRSIIQHADDPNTKDNEIIQKIMNFSTEFLIKFYGKLIKDLGGIDKFIRNDD 428          

HSP 2 Score: 91.2781 bits (225), Expect = 3.952e-19
Identity = 90/321 (28.04%), Postives = 150/321 (46.73%), Query Frame = 2
Query:  332 MSIETIVTKHNYNCETVFKTFVTRLPDGKKLKTQRSFTREISN--YSDIKFKLRNQFSVVISDDEPVIQKPDVARPPTKIKVKKRPSFKAFTXXXXXXXNDKQDAPRHKSGAHPEEEHKVLQPMXXXXXXXXXXXXXXXXXRTVSIEDKLSIIPPTGRGEGDSKQSVMQEVKTFSTEKNEMEKEKPTNILAKISLKNGTKIFEIIN---DNIPFIXXXXXXXXXXXXXXXXXXXXXXXGSFLHFDFAVSSDIRNTINRIIKDMSCDCEKKKFNMISDSLTYIAKKDQESKKQIWLEIKRFDNTKKHFIDCAVWICNKKNAN 1279
            MS++T++T+ N  CETVF T VT+  +GK+L + RSFTREI +  ++D    + + F VV SD+E    K   ++     K  K+P  K+    K SK    +     K   +P     V++P+   +                    KLS+             + MQ+   FSTE N    +KP NILAKIS+ N T  FE+I+   D+ PF+D+  + +   E      ++     S+L F+ +  ++       + KD +C+CEK++ +++ + + +  +   E K      +  F+   +HF DC       KN+N
Sbjct:    1 MSVQTVITRANSECETVFMTSVTKAQNGKRLTSHRSFTREIVDCQHNDDCINIHDNFEVVFSDNENSPGKKTNSKKNIVDKTFKKPFLKSIKLPKLSKKKSLEKRDELK---YP----TVIKPILGNR--------------------KLSL-------------ACMQD---FSTEFNPTMNQKPRNILAKISVSNKTHDFEVIDEVIDDTPFMDEENENDINNESAPNLQEASPTSDSYLQFNLSTCNNSNTETADLFKDTTCNCEKRRISVLVEFVEWKLRHRSEFKND---SLNTFNEQTRHFSDCHKLSSGIKNSN 275          
The following BLAST results are available for this feature:
BLAST of SMED30019787 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019787 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019787 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019787 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019787 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019787 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019787 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019787 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019787 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019787 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019787 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019787 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019787 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019787 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000048292.10.000e+099.60SMESG000048292.1[more]
SMESG000048292.10.000e+099.60SMESG000048292.1[more]
SMESG000048297.11.171e-3831.50SMESG000048297.1[more]
SMESG000048287.17.842e-2435.19SMESG000048287.1[more]
SMESG000048297.12.876e-2029.39SMESG000048297.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30019787 ID=SMED30019787|Name=SMED30019787|organism=Schmidtea mediterranea sexual|type=transcript|length=2479bp
TATATAAATCTTATATGTGATGATGGACCTAAATAACGATCTTGTGTCGT
TGTTGATTACCTACATAGAACAGGAATCTGAAGAAGAGCAAAGATTTATT
GAAGATCAGGAAAATTGTGAATTGAAACTCTGCTTATCAGTAAGTCATAG
CAACTGCGGATGGAATGATTGTGGAACGGATTTGATAATGATACACACTA
GATTGAACCAATCAAATGAGTTCATTGAGGAAAACATTGATCAGGTACCT
AATCAATTCTATATCAAATCACAGATTGTCTCTCATTGCACTGGCATGTT
AATTACTGACATCGGTGGAGCCAATAAAATTATGTCAATTGAGACAATTG
TCACAAAACATAATTACAACTGTGAAACCGTATTCAAGACATTTGTCACC
AGATTACCTGATGGTAAGAAACTGAAAACTCAGCGTTCGTTTACGAGAGA
AATTTCCAATTATAGTGATATCAAATTCAAACTGCGAAATCAATTTTCAG
TTGTCATCTCAGACGATGAACCCGTGATCCAGAAACCCGATGTGGCCAGG
CCTCCAACAAAAATAAAAGTTAAGAAAAGGCCTTCCTTCAAAGCATTCAC
AAAATCCAAGAAATCCAAATCCAATGACAAACAAGATGCTCCTCGTCACA
AAAGTGGAGCACATCCTGAAGAGGAGCATAAAGTTTTGCAACCCATGAAA
AAACAGAAATCTAGTATTTTTAAAAGTTTTAAGAATATTTTCAAATCCCG
TACCGTTTCTATCGAGGACAAGCTATCTATCATCCCTCCAACTGGTCGAG
GCGAAGGAGATTCTAAACAATCAGTGATGCAGGAAGTAAAAACCTTCTCA
ACCGAAAAAAATGAAATGGAAAAAGAAAAACCGACAAATATATTAGCAAA
AATCAGCCTGAAAAATGGAACAAAGATATTCGAAATTATCAACGACAATA
TTCCATTTATCGATGATGTCGAAGATGAAGAGTCTGAAGAGGAAGAATGC
GTGAAAAGTTCAAAATCAGATTCTGATTGCGGATCGTTTTTGCATTTTGA
TTTCGCAGTTTCGTCGGACATTCGAAACACAATAAACCGAATTATCAAGG
ACATGAGTTGTGACTGTGAAAAAAAAAAGTTCAACATGATTTCAGATTCA
TTGACGTATATCGCAAAAAAGGACCAAGAATCAAAGAAACAAATTTGGCT
AGAAATCAAACGATTTGACAATACAAAGAAACATTTTATCGACTGTGCTG
TTTGGATTTGCAATAAAAAGAATGCGAATTATCAAAGGAAATCACTTCCG
CCTAATGAAGATTTTCATGATTTGTGCCGCATTTGCCATGACATAGATCA
GCATTTTAATGAAAATCTTTGTACCATTTTTGTTCCATTTTTAAAAGAAA
TCGAAACGAAGGAAAGACAAGAATTGTGTTTATGCGAAAGAAAAAAGAAA
GAGAAGATAAAATCTTTTATTTATTCGACCATGACCTCTGTGAAAATCAA
TGATAATTTCAGAGATTCATTTAACAAGGTCACGAATCATTTCATGGACT
GTGTTTGTAACTGGATTTCTCCTGCCATCAATCCTTCAATAGTTGACACA
AATAACAATTTGGATGATAAAATAGAGACGACCTCTTGTAACTGTTACAA
GCGTCGGATGCAAATTCTTGCCCATTTTATTGGTCACATATTCTTCAAAA
AGGCTTTGAGAACTGATGACGGCAACGAATTCGGTCTAAAAACCAAACAC
TCTACGAGTTGTCCAAGATATTTATCTACCAGTGGCACATCACCTATAGC
CTATACACCATCTCCCTTCAGTAATGGTTATCCAGTGTTTGAATCATTTT
CTCCGACTATTTCTCCTACTCAGGGCAAAAAGCATTCTTCAGAATCAAAA
AGAAAAAAATCAAGAACGAATAGCTTTAGTTCAATGCATTCAATCAAAAG
CCTTAATGACGTGATTCAAAGTATTAAGAATGGCTATGAGATTGAATGTT
CGATTGAAACGATTGAATACTTTATAAAGTGTAAAGATTATGATAGCTTC
GACAAAGGAATATTTCATGTTACCGAAGAAAATGTATCCATAGAAAACAT
GTGCTTATTTTTTTATTTGATACGCAAGATTGTGAGATATATAGATGATC
CCAGTTGTGAGGATAATGAGGATATCAAGAGGATTATCGAATATTCAGAG
AAATATCTTTTGAAATATTATCTAGATGAGATCAGTAAACAAGGGGGTTT
GGAGAAATTTATATTGGGAATTGATAGCCTGGATTAGCAAGTAATAGAAA
ATTGATTCCAAAGTGCCAAGTTATTTGGTCTAATATTTCCTATGAAAAAC
GACTGACAGCATTTGACTTTTCGATGACGATGCCGCAGATATTTAGAGAT
ATTTTATTCGACAGACTTTAGAGATTTCTATATTTTTATTCGAAACAATA
ATAATTTTTAAATGATTCTATTTAATGGA
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protein sequence of SMED30019787-orf-1

>SMED30019787-orf-1 ID=SMED30019787-orf-1|Name=SMED30019787-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=756bp
MMDLNNDLVSLLITYIEQESEEEQRFIEDQENCELKLCLSVSHSNCGWND
CGTDLIMIHTRLNQSNEFIEENIDQVPNQFYIKSQIVSHCTGMLITDIGG
ANKIMSIETIVTKHNYNCETVFKTFVTRLPDGKKLKTQRSFTREISNYSD
IKFKLRNQFSVVISDDEPVIQKPDVARPPTKIKVKKRPSFKAFTKSKKSK
SNDKQDAPRHKSGAHPEEEHKVLQPMKKQKSSIFKSFKNIFKSRTVSIED
KLSIIPPTGRGEGDSKQSVMQEVKTFSTEKNEMEKEKPTNILAKISLKNG
TKIFEIINDNIPFIDDVEDEESEEEECVKSSKSDSDCGSFLHFDFAVSSD
IRNTINRIIKDMSCDCEKKKFNMISDSLTYIAKKDQESKKQIWLEIKRFD
NTKKHFIDCAVWICNKKNANYQRKSLPPNEDFHDLCRICHDIDQHFNENL
CTIFVPFLKEIETKERQELCLCERKKKEKIKSFIYSTMTSVKINDNFRDS
FNKVTNHFMDCVCNWISPAINPSIVDTNNNLDDKIETTSCNCYKRRMQIL
AHFIGHIFFKKALRTDDGNEFGLKTKHSTSCPRYLSTSGTSPIAYTPSPF
SNGYPVFESFSPTISPTQGKKHSSESKRKKSRTNSFSSMHSIKSLNDVIQ
SIKNGYEIECSIETIEYFIKCKDYDSFDKGIFHVTEENVSIENMCLFFYL
IRKIVRYIDDPSCEDNEDIKRIIEYSEKYLLKYYLDEISKQGGLEKFILG
IDSLD*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002032epidermal cell
Vocabulary: INTERPRO
TermDefinition
IPR012317Poly(ADP-ribose)pol_cat_dom
Vocabulary: molecular function
TermDefinition
GO:0003950NAD+ ADP-ribosyltransferase activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 267..287
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 173..231
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 197..227
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 614..638