Afadin
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30019772 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 1
Homology
BLAST of Afadin vs. Ensembl Human
Match: AFDN (afadin, adherens junction formation factor [Source:HGNC Symbol;Acc:HGNC:7137]) HSP 1 Score: 153.68 bits (387), Expect = 9.825e-37 Identity = 127/412 (30.83%), Postives = 200/412 (48.54%), Query Frame = 1
Query: 1567 LPVQITYFKSKEDMLLDSILLDIKPSQTKFKLASAYLLHLMVVDIIRLTCINRATVLDQELVIGMFIEKVASKIRQSIMQHRTIISIQSYWLANCTEFLHLLKNNILLSQFNSSIFDKYYDLFADNAQWCFQCISFGFTDDMVNTLSVLLNENTDDSEEKDDFPLSRKASDPKNMSILIRTLSSCMSVLQQCHVNPALIIQIISRIFHLINCWIFNSFIDTP----CRIFRGWAXXXXXXXXXXXXWSERHDLLLAADCYFQQSLQACKLLIMEKTDIEVFYNETVDMVDINSIQLRYLLDNYIYSPNEPKVPQIWIDFVVSGANQVADKVFEEEGIDICXXXXXXXXXXXXXXXDGYHVDSLYGGVPVGFIEYLEKLHKEWCFYKISHQDIHFDWLCQKRGFQDTSCELLP 2790
LP I + +S ED L +I+ S FKL+ Y+L++ ++ + ++ + + K+ S + I + + I ++W+AN +E L+ +K + LS+ I D+ A Q F+ + ++ N + L DD EE L R PK + ++ TL+ MS+L++C VN AL IQ+ S++FH IN W+FN + P C + W II ++ I W+E+ L LAADC+ + +QA LL M+K + N +NS+QL+ LL NY +P+EP +P I+ VV+ A AD++ +G ++ L+ L LP LLPEDGY D + +P G E+L D LCQ RGF C L+P
Sbjct: 560 LPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSR----ITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFL----DDPEENS---LQR----PK-IDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHY--WGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCD-VVRNIPNGLQEFL-------------------DPLCQ-RGF----CRLIP 928 HSP 2 Score: 103.219 bits (256), Expect = 3.060e-21 Identity = 104/342 (30.41%), Postives = 157/342 (45.91%), Query Frame = 1
Query: 202 MRFFFE--TEDKFHSKCLRVSSDLKVKDLLPILLEKFYVSDICSSKQDFRLFE-RSSSIDKLLDPEDFPLLIQLFWC----------------------------KDDKEGVFV-FKSNLKNKNSRR--------IRSQTRKD--KIGSTTCLNSPTTRCLDKVCTDPPTKSTKQXXXXXXXXXXXXXXXRTISNPXXXXXXXXXXXXXXXXXQISKS-GNLNGGGTLKIYGDSLNPLIPYKSLLLSIDDTAEFVVREILDKYGMENENENDFSLVSSVY---KDQNTQKIEYEEILQNSDSPLRFLLQSNKNDGFIKFRVVKMPAN-ISKSKKSKTQGTFP 1086
MRF+F+ F +KC+RVSS +D++ L EKF S + L+E S ++ LD ++ PL++QL W K +KEGV FK L K + +R + KD NS R +V D P S +RTISNPE +M+ +R+ KLE+++++ S G + GGTL+IY DSL P IPYK++LLS D A+F V E L+KYG+E EN D+ + + + +K E IL + + PL+ + + G + F++ + P + I K K +G P
Sbjct: 1 MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENS---RLAAEVYKDMPETS----------------FTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTP 323
BLAST of Afadin vs. Ensembl Human
Match: AFDN (afadin, adherens junction formation factor [Source:HGNC Symbol;Acc:HGNC:7137]) HSP 1 Score: 153.68 bits (387), Expect = 1.049e-36 Identity = 127/412 (30.83%), Postives = 200/412 (48.54%), Query Frame = 1
Query: 1567 LPVQITYFKSKEDMLLDSILLDIKPSQTKFKLASAYLLHLMVVDIIRLTCINRATVLDQELVIGMFIEKVASKIRQSIMQHRTIISIQSYWLANCTEFLHLLKNNILLSQFNSSIFDKYYDLFADNAQWCFQCISFGFTDDMVNTLSVLLNENTDDSEEKDDFPLSRKASDPKNMSILIRTLSSCMSVLQQCHVNPALIIQIISRIFHLINCWIFNSFIDTP----CRIFRGWAXXXXXXXXXXXXWSERHDLLLAADCYFQQSLQACKLLIMEKTDIEVFYNETVDMVDINSIQLRYLLDNYIYSPNEPKVPQIWIDFVVSGANQVADKVFEEEGIDICXXXXXXXXXXXXXXXDGYHVDSLYGGVPVGFIEYLEKLHKEWCFYKISHQDIHFDWLCQKRGFQDTSCELLP 2790
LP I + +S ED L +I+ S FKL+ Y+L++ ++ + ++ + + K+ S + I + + I ++W+AN +E L+ +K + LS+ I D+ A Q F+ + ++ N + L DD EE L R PK + ++ TL+ MS+L++C VN AL IQ+ S++FH IN W+FN + P C + W II ++ I W+E+ L LAADC+ + +QA LL M+K + N +NS+QL+ LL NY +P+EP +P I+ VV+ A AD++ +G ++ L+ L LP LLPEDGY D + +P G E+L D LCQ RGF C L+P
Sbjct: 601 LPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSR----ITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFL----DDPEENS---LQR----PK-IDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHY--WGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCD-VVRNIPNGLQEFL-------------------DPLCQ-RGF----CRLIP 969 HSP 2 Score: 142.124 bits (357), Expect = 3.461e-33 Identity = 119/368 (32.34%), Postives = 181/368 (49.18%), Query Frame = 1
Query: 121 LINQWNSSRLELFKISQPDKNLKFFGVMRFFFE--TEDKFHSKCLRVSSDLKVKDLLPILLEKFYVSDICSSKQDFRLFERSSSIDKLLDPEDFPLLIQLFWC----------------------------KDDKEGVFV-FKSNLKNKNSRR--------IRSQTRKD--KIGSTTCLNSPTTRCLDKVCTDPPTKSTKQXXXXXXXXXXXXXXXRTISNPXXXXXXXXXXXXXXXXXQISKS-GNLNGGGTLKIYGDSLNPLIPYKSLLLSIDDTAEFVVREILDKYGMENENENDFSLVSSVY---KDQNTQKIEYEEILQNSDSPLRFLLQSNKNDGFIKFRVVKMPAN-ISKSKKSKTQGTFP 1086
+I+ WN++RL+LF+ISQP ++L+F GVMRF+F+ F +KC+RVSS +D++ L EKF S + L+E S ++ LD ++ PL++QL W K +KEGV FK L K + +R + KD NS R +V D P S +RTISNPE +M+ +R+ KLE+++++ S G + GGTL+IY DSL P IPYK++LLS D A+F V E L+KYG+E EN D+ + + + +K E IL + + PL+ + + G + F++ + P + I K K +G P
Sbjct: 16 IIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENS---RLAAEVYKDMPETS----------------FTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTP 364
BLAST of Afadin vs. Ensembl Human
Match: AFDN (afadin, adherens junction formation factor [Source:HGNC Symbol;Acc:HGNC:7137]) HSP 1 Score: 153.68 bits (387), Expect = 1.089e-36 Identity = 127/412 (30.83%), Postives = 200/412 (48.54%), Query Frame = 1
Query: 1567 LPVQITYFKSKEDMLLDSILLDIKPSQTKFKLASAYLLHLMVVDIIRLTCINRATVLDQELVIGMFIEKVASKIRQSIMQHRTIISIQSYWLANCTEFLHLLKNNILLSQFNSSIFDKYYDLFADNAQWCFQCISFGFTDDMVNTLSVLLNENTDDSEEKDDFPLSRKASDPKNMSILIRTLSSCMSVLQQCHVNPALIIQIISRIFHLINCWIFNSFIDTP----CRIFRGWAXXXXXXXXXXXXWSERHDLLLAADCYFQQSLQACKLLIMEKTDIEVFYNETVDMVDINSIQLRYLLDNYIYSPNEPKVPQIWIDFVVSGANQVADKVFEEEGIDICXXXXXXXXXXXXXXXDGYHVDSLYGGVPVGFIEYLEKLHKEWCFYKISHQDIHFDWLCQKRGFQDTSCELLP 2790
LP I + +S ED L +I+ S FKL+ Y+L++ ++ + ++ + + K+ S + I + + I ++W+AN +E L+ +K + LS+ I D+ A Q F+ + ++ N + L DD EE L R PK + ++ TL+ MS+L++C VN AL IQ+ S++FH IN W+FN + P C + W II ++ I W+E+ L LAADC+ + +QA LL M+K + N +NS+QL+ LL NY +P+EP +P I+ VV+ A AD++ +G ++ L+ L LP LLPEDGY D + +P G E+L D LCQ RGF C L+P
Sbjct: 600 LPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSR----ITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFL----DDPEENS---LQR----PK-IDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHY--WGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCD-VVRNIPNGLQEFL-------------------DPLCQ-RGF----CRLIP 968 HSP 2 Score: 145.206 bits (365), Expect = 4.247e-34 Identity = 120/367 (32.70%), Postives = 182/367 (49.59%), Query Frame = 1
Query: 121 LINQWNSSRLELFKISQPDKNLKFFGVMRFFFE--TEDKFHSKCLRVSSDLKVKDLLPILLEKFYVSDICSSKQDFRLFERSSSIDKLLDPEDFPLLIQLFWCKD---------------------------DKEGVFV-FKSNLKNKNSRR--------IRSQTRKD--KIGSTTCLNSPTTRCLDKVCTDPPTKSTKQXXXXXXXXXXXXXXXRTISNPXXXXXXXXXXXXXXXXXQISKS-GNLNGGGTLKIYGDSLNPLIPYKSLLLSIDDTAEFVVREILDKYGMENENENDFSLVSSVY---KDQNTQKIEYEEILQNSDSPLRFLLQSNKNDGFIKFRVVKMPAN-ISKSKKSKTQGTFP 1086
+I+ WN++RL+LF+ISQP ++L+F GVMRF+F+ F +KC+RVSS +D++ L EKF S + L+E S ++ LD ++ PL++QL W KD +KEGV FK L K + +R + KD NS R +V D P S +RTISNPE +M+ +R+ KLE+++++ S G + GGTL+IY DSL P IPYK++LLS D A+F V E L+KYG+E EN D+ + + + +K E IL + + PL+ + + G + F++ + P + I K K +G P
Sbjct: 16 IIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENS---RLAAEVYKDMPETS----------------FTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTP 363
BLAST of Afadin vs. Ensembl Human
Match: AFDN (afadin, adherens junction formation factor [Source:HGNC Symbol;Acc:HGNC:7137]) HSP 1 Score: 153.295 bits (386), Expect = 1.326e-36 Identity = 127/412 (30.83%), Postives = 200/412 (48.54%), Query Frame = 1
Query: 1567 LPVQITYFKSKEDMLLDSILLDIKPSQTKFKLASAYLLHLMVVDIIRLTCINRATVLDQELVIGMFIEKVASKIRQSIMQHRTIISIQSYWLANCTEFLHLLKNNILLSQFNSSIFDKYYDLFADNAQWCFQCISFGFTDDMVNTLSVLLNENTDDSEEKDDFPLSRKASDPKNMSILIRTLSSCMSVLQQCHVNPALIIQIISRIFHLINCWIFNSFIDTP----CRIFRGWAXXXXXXXXXXXXWSERHDLLLAADCYFQQSLQACKLLIMEKTDIEVFYNETVDMVDINSIQLRYLLDNYIYSPNEPKVPQIWIDFVVSGANQVADKVFEEEGIDICXXXXXXXXXXXXXXXDGYHVDSLYGGVPVGFIEYLEKLHKEWCFYKISHQDIHFDWLCQKRGFQDTSCELLP 2790
LP I + +S ED L +I+ S FKL+ Y+L++ ++ + ++ + + K+ S + I + + I ++W+AN +E L+ +K + LS+ I D+ A Q F+ + ++ N + L DD EE L R PK + ++ TL+ MS+L++C VN AL IQ+ S++FH IN W+FN + P C + W II ++ I W+E+ L LAADC+ + +QA LL M+K + N +NS+QL+ LL NY +P+EP +P I+ VV+ A AD++ +G ++ L+ L LP LLPEDGY D + +P G E+L D LCQ RGF C L+P
Sbjct: 585 LPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSR----ITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFL----DDPEENS---LQR----PK-IDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHY--WGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCD-VVRNIPNGLQEFL-------------------DPLCQ-RGF----CRLIP 953 HSP 2 Score: 142.51 bits (358), Expect = 2.731e-33 Identity = 119/367 (32.43%), Postives = 181/367 (49.32%), Query Frame = 1
Query: 121 LINQWNSSRLELFKISQPDKNLKFFGVMRFFFE--TEDKFHSKCLRVSSDLKVKDLLPILLEKFYVSDICSSKQDFRLFERSSSIDKLLDPEDFPLLIQLFWC---------------------------KDDKEGVFV-FKSNLKNKNSRR--------IRSQTRKD--KIGSTTCLNSPTTRCLDKVCTDPPTKSTKQXXXXXXXXXXXXXXXRTISNPXXXXXXXXXXXXXXXXXQISKS-GNLNGGGTLKIYGDSLNPLIPYKSLLLSIDDTAEFVVREILDKYGMENENENDFSLVSSVY---KDQNTQKIEYEEILQNSDSPLRFLLQSNKNDGFIKFRVVKMPAN-ISKSKKSKTQGTFP 1086
+I+ WN++RL+LF+ISQP ++L+F GVMRF+F+ F +KC+RVSS +D++ L EKF S + L+E S ++ LD ++ PL++QL W K +KEGV FK L K + +R + KD NS R +V D P S +RTISNPE +M+ +R+ KLE+++++ S G + GGTL+IY DSL P IPYK++LLS D A+F V E L+KYG+E EN D+ + + + +K E IL + + PL+ + + G + F++ + P + I K K +G P
Sbjct: 16 IIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENS---RLAAEVYKDMPETS----------------FTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTP 363
BLAST of Afadin vs. Ensembl Human
Match: AFDN (afadin, adherens junction formation factor [Source:HGNC Symbol;Acc:HGNC:7137]) HSP 1 Score: 152.91 bits (385), Expect = 1.905e-36 Identity = 127/412 (30.83%), Postives = 200/412 (48.54%), Query Frame = 1
Query: 1567 LPVQITYFKSKEDMLLDSILLDIKPSQTKFKLASAYLLHLMVVDIIRLTCINRATVLDQELVIGMFIEKVASKIRQSIMQHRTIISIQSYWLANCTEFLHLLKNNILLSQFNSSIFDKYYDLFADNAQWCFQCISFGFTDDMVNTLSVLLNENTDDSEEKDDFPLSRKASDPKNMSILIRTLSSCMSVLQQCHVNPALIIQIISRIFHLINCWIFNSFIDTP----CRIFRGWAXXXXXXXXXXXXWSERHDLLLAADCYFQQSLQACKLLIMEKTDIEVFYNETVDMVDINSIQLRYLLDNYIYSPNEPKVPQIWIDFVVSGANQVADKVFEEEGIDICXXXXXXXXXXXXXXXDGYHVDSLYGGVPVGFIEYLEKLHKEWCFYKISHQDIHFDWLCQKRGFQDTSCELLP 2790
LP I + +S ED L +I+ S FKL+ Y+L++ ++ + ++ + + K+ S + I + + I ++W+AN +E L+ +K + LS+ I D+ A Q F+ + ++ N + L DD EE L R PK + ++ TL+ MS+L++C VN AL IQ+ S++FH IN W+FN + P C + W II ++ I W+E+ L LAADC+ + +QA LL M+K + N +NS+QL+ LL NY +P+EP +P I+ VV+ A AD++ +G ++ L+ L LP LLPEDGY D + +P G E+L D LCQ RGF C L+P
Sbjct: 601 LPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSR----ITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFL----DDPEENS---LQR----PK-IDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHY--WGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCD-VVRNIPNGLQEFL-------------------DPLCQ-RGF----CRLIP 969 HSP 2 Score: 142.51 bits (358), Expect = 2.689e-33 Identity = 119/368 (32.34%), Postives = 181/368 (49.18%), Query Frame = 1
Query: 121 LINQWNSSRLELFKISQPDKNLKFFGVMRFFFE--TEDKFHSKCLRVSSDLKVKDLLPILLEKFYVSDICSSKQDFRLFERSSSIDKLLDPEDFPLLIQLFWC----------------------------KDDKEGVFV-FKSNLKNKNSRR--------IRSQTRKD--KIGSTTCLNSPTTRCLDKVCTDPPTKSTKQXXXXXXXXXXXXXXXRTISNPXXXXXXXXXXXXXXXXXQISKS-GNLNGGGTLKIYGDSLNPLIPYKSLLLSIDDTAEFVVREILDKYGMENENENDFSLVSSVY---KDQNTQKIEYEEILQNSDSPLRFLLQSNKNDGFIKFRVVKMPAN-ISKSKKSKTQGTFP 1086
+I+ WN++RL+LF+ISQP ++L+F GVMRF+F+ F +KC+RVSS +D++ L EKF S + L+E S ++ LD ++ PL++QL W K +KEGV FK L K + +R + KD NS R +V D P S +RTISNPE +M+ +R+ KLE+++++ S G + GGTL+IY DSL P IPYK++LLS D A+F V E L+KYG+E EN D+ + + + +K E IL + + PL+ + + G + F++ + P + I K K +G P
Sbjct: 16 IIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENS---RLAAEVYKDMPETS----------------FTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTP 364
BLAST of Afadin vs. Ensembl Celegans
Match: afd-1 (AFaDin (Actin filament binding protein) homolog [Source:UniProtKB/TrEMBL;Acc:X5M8U5]) HSP 1 Score: 108.612 bits (270), Expect = 2.049e-23 Identity = 100/332 (30.12%), Postives = 162/332 (48.80%), Query Frame = 1
Query: 121 LINQWNSSRLELFKISQPDKNLKFFGVMRFFFE-TEDKFHSKCLRVSSDLKVKDLLPILLEKFYVSDICSSKQDFRLFE--RSSSIDKLLDPEDFPLLIQLFWCKDDKEGVFVFKS---------NLKNKNSRRIR----------SQTRKDKIGSTTCLNSPTTRCLDKVCTDPPTKSTKQXXXXXXXXXXXXXXXRTISNPXXXXXXXXXXXXXXXXXQISKSGNLNGGGTLKIYGDSLNPLIPYKSLLLSIDDTAEFVVREILDKYGMENENENDFSLVSSVYKDQNTQKIEYEEI----LQNSDSPLRFLLQ--SNKNDGFIKFRVVK 1032
L+ QWN +RL+LF +S P ++L+ GVMRF+F+ +K +KC+RVSS + ++ L EKF S + L+E + KLLD E+ PL++QL W KDD+EG F+ K NL+ + + R + +K+ + + D + P + +RTISNPE++MR +R+ KLE +K + GG+LK+YG + P PY S+L I++ A+ ++ L+KYG+E+ +++DF LV D + EI + ++ PL + N +GF F +K
Sbjct: 11 LVQQWNENRLDLFHLSYPTEDLEVEGVMRFYFQDGGEKVLTKCVRVSSTATTRAVVDALSEKFLPDLKMLSNDTYSLWEVHENGEERKLLD-EEKPLVVQLQWHKDDREGRFLLKKDAAPPVSLLNLQEADQKNTRFAKKKDKEPKKKNQKNFVSVQVNGGREEAQSQDDIYKQVPFNT----------------FTRTISNPEAVMRKRREKKLE------TKLKEMMFGGSLKVYGGEIVPTRPYVSILAEINENADRILGAALEKYGLEH-SKDDFILVEVSNDDDRKSMSDLREIDGRPIPPTECPLFEMTARSGNGENGFDSFLAIK 318 HSP 2 Score: 78.5666 bits (192), Expect = 3.063e-14 Identity = 78/296 (26.35%), Postives = 146/296 (49.32%), Query Frame = 1
Query: 1834 YWLANCTEFLHLLKNNILLSQFNSSIFDKYYDLFADNAQWCFQCISFGFTDDMVNTLSVLLNENTDDSEEKDDFPLSRKASDPKNMSILIRTLSSCMSVLQQCHVNPALIIQIISRIFHLINCWIFNSFIDT---PCRIFRGWAXXXXXXXXXXXXWSERHDLLLAADCYFQQSLQACKLLIMEKTDIEVFYNETVDMVDINSIQLRYLLDNYIYSPNEPKVP--QIWIDFVVSGANQVADKVFEEEGIDICXXXXXXXXXXXXXXXDGYHVDSLYGGVPVGFIEYLEKL-HKEWC 2703
+WLAN +EF HL++ + ++ +S + K + + F+ + + LL+ + +D + D +I L + M + C +N AL IQ+ + H++N ++FNS + ++ + + RIE I R+ ER + +AA+C+ +S QA LL +K D+ + + NS+Q+ ++L + P++ ++P +D + A + ADK+ E+G + L+ + LNLP L+P+DGY V+ + VP G +YL++L H+ C
Sbjct: 633 FWLANASEFNHLVERD---TELKASRYGK----ISVEIERIFRRLVAVLQSVIRPAARALLDIHLNDHDATGD---------------VIHLLDTTMRQARICGLNAALTIQLCGHLLHMVNAFVFNSLVSVNLPAAQLNTRLGKCLQYRIESIHRFCERMGVEMAAECHLDRSRQAANLLATQKNDVAALGSTCYKL---NSLQVVHILSG--FEPDDGEIPCDDDVVDRICRLAEKQADKLTLEDGQRVTLEESDELNLPFLIPQDGYFVEQ-FRTVPDGLWQYLQQLQHRRLC 900
BLAST of Afadin vs. Ensembl Celegans
Match: afd-1 (AFaDin (Actin filament binding protein) homolog [Source:UniProtKB/TrEMBL;Acc:X5M8U5]) HSP 1 Score: 108.612 bits (270), Expect = 2.247e-23 Identity = 98/314 (31.21%), Postives = 159/314 (50.64%), Query Frame = 1
Query: 121 LINQWNSSRLELFKISQPDKNLKFFGVMRFFFE-TEDKFHSKCLRVSSDLKVKDLLPILLEKFYVSDICSSKQDFRLFE--RSSSIDKLLDPEDFPLLIQLFWCKDDKEGVFVFKSNLKNKNSR-RIRSQTRKDKIGSTTCLNSPTTRCLDKVCTDPPTKSTKQXXXXXXXXXXXXXXXRTISNPXXXXXXXXXXXXXXXXXQISKSGNLNGGGTLKIYGDSLNPLIPYKSLLLSIDDTAEFVVREILDKYGMENENENDFSLVSSVYKDQNTQKIEYEEI----LQNSDSPLRFLLQ--SNKNDGFIKFRVVK 1032
L+ QWN +RL+LF +S P ++L+ GVMRF+F+ +K +KC+RVSS + ++ L EKF S + L+E + KLLD E+ PL++QL W KDD+EG F+ K + S ++ +K+ + P + + Q + +RTISNPE++MR +R+ KLE +K + GG+LK+YG + P PY S+L I++ A+ ++ L+KYG+E+ +++DF LV D + EI + ++ PL + N +GF F +K
Sbjct: 11 LVQQWNENRLDLFHLSYPTEDLEVEGVMRFYFQDGGEKVLTKCVRVSSTATTRAVVDALSEKFLPDLKMLSNDTYSLWEVHENGEERKLLD-EEKPLVVQLQWHKDDREGRFLLKKDAAPPVSLLNLQEADQKNTRFAKKKDKEPKKKNQKNFVQVNGGREEAQSQDDIYKQVPFNTFTRTISNPEAVMRKRREKKLE------TKLKEMMFGGSLKVYGGEIVPTRPYVSILAEINENADRILGAALEKYGLEH-SKDDFILVEVSNDDDRKSMSDLREIDGRPIPPTECPLFEMTARSGNGENGFDSFLAIK 316 HSP 2 Score: 78.5666 bits (192), Expect = 3.062e-14 Identity = 78/296 (26.35%), Postives = 146/296 (49.32%), Query Frame = 1
Query: 1834 YWLANCTEFLHLLKNNILLSQFNSSIFDKYYDLFADNAQWCFQCISFGFTDDMVNTLSVLLNENTDDSEEKDDFPLSRKASDPKNMSILIRTLSSCMSVLQQCHVNPALIIQIISRIFHLINCWIFNSFIDT---PCRIFRGWAXXXXXXXXXXXXWSERHDLLLAADCYFQQSLQACKLLIMEKTDIEVFYNETVDMVDINSIQLRYLLDNYIYSPNEPKVP--QIWIDFVVSGANQVADKVFEEEGIDICXXXXXXXXXXXXXXXDGYHVDSLYGGVPVGFIEYLEKL-HKEWC 2703
+WLAN +EF HL++ + ++ +S + K + + F+ + + LL+ + +D + D +I L + M + C +N AL IQ+ + H++N ++FNS + ++ + + RIE I R+ ER + +AA+C+ +S QA LL +K D+ + + NS+Q+ ++L + P++ ++P +D + A + ADK+ E+G + L+ + LNLP L+P+DGY V+ + VP G +YL++L H+ C
Sbjct: 631 FWLANASEFNHLVERD---TELKASRYGK----ISVEIERIFRRLVAVLQSVIRPAARALLDIHLNDHDATGD---------------VIHLLDTTMRQARICGLNAALTIQLCGHLLHMVNAFVFNSLVSVNLPAAQLNTRLGKCLQYRIESIHRFCERMGVEMAAECHLDRSRQAANLLATQKNDVAALGSTCYKL---NSLQVVHILSG--FEPDDGEIPCDDDVVDRICRLAEKQADKLTLEDGQRVTLEESDELNLPFLIPQDGYFVEQ-FRTVPDGLWQYLQQLQHRRLC 898
BLAST of Afadin vs. Ensembl Celegans
Match: afd-1 (AFaDin (Actin filament binding protein) homolog [Source:UniProtKB/TrEMBL;Acc:X5M8U5]) HSP 1 Score: 108.612 bits (270), Expect = 2.550e-23 Identity = 98/314 (31.21%), Postives = 159/314 (50.64%), Query Frame = 1
Query: 121 LINQWNSSRLELFKISQPDKNLKFFGVMRFFFE-TEDKFHSKCLRVSSDLKVKDLLPILLEKFYVSDICSSKQDFRLFE--RSSSIDKLLDPEDFPLLIQLFWCKDDKEGVFVFKSNLKNKNSR-RIRSQTRKDKIGSTTCLNSPTTRCLDKVCTDPPTKSTKQXXXXXXXXXXXXXXXRTISNPXXXXXXXXXXXXXXXXXQISKSGNLNGGGTLKIYGDSLNPLIPYKSLLLSIDDTAEFVVREILDKYGMENENENDFSLVSSVYKDQNTQKIEYEEI----LQNSDSPLRFLLQ--SNKNDGFIKFRVVK 1032
L+ QWN +RL+LF +S P ++L+ GVMRF+F+ +K +KC+RVSS + ++ L EKF S + L+E + KLLD E+ PL++QL W KDD+EG F+ K + S ++ +K+ + P + + Q + +RTISNPE++MR +R+ KLE +K + GG+LK+YG + P PY S+L I++ A+ ++ L+KYG+E+ +++DF LV D + EI + ++ PL + N +GF F +K
Sbjct: 11 LVQQWNENRLDLFHLSYPTEDLEVEGVMRFYFQDGGEKVLTKCVRVSSTATTRAVVDALSEKFLPDLKMLSNDTYSLWEVHENGEERKLLD-EEKPLVVQLQWHKDDREGRFLLKKDAAPPVSLLNLQEADQKNTRFAKKKDKEPKKKNQKNFVQVNGGREEAQSQDDIYKQVPFNTFTRTISNPEAVMRKRREKKLE------TKLKEMMFGGSLKVYGGEIVPTRPYVSILAEINENADRILGAALEKYGLEH-SKDDFILVEVSNDDDRKSMSDLREIDGRPIPPTECPLFEMTARSGNGENGFDSFLAIK 316 HSP 2 Score: 78.9518 bits (193), Expect = 2.882e-14 Identity = 78/296 (26.35%), Postives = 146/296 (49.32%), Query Frame = 1
Query: 1834 YWLANCTEFLHLLKNNILLSQFNSSIFDKYYDLFADNAQWCFQCISFGFTDDMVNTLSVLLNENTDDSEEKDDFPLSRKASDPKNMSILIRTLSSCMSVLQQCHVNPALIIQIISRIFHLINCWIFNSFIDT---PCRIFRGWAXXXXXXXXXXXXWSERHDLLLAADCYFQQSLQACKLLIMEKTDIEVFYNETVDMVDINSIQLRYLLDNYIYSPNEPKVP--QIWIDFVVSGANQVADKVFEEEGIDICXXXXXXXXXXXXXXXDGYHVDSLYGGVPVGFIEYLEKL-HKEWC 2703
+WLAN +EF HL++ + ++ +S + K + + F+ + + LL+ + +D + D +I L + M + C +N AL IQ+ + H++N ++FNS + ++ + + RIE I R+ ER + +AA+C+ +S QA LL +K D+ + + NS+Q+ ++L + P++ ++P +D + A + ADK+ E+G + L+ + LNLP L+P+DGY V+ + VP G +YL++L H+ C
Sbjct: 631 FWLANASEFNHLVERD---TELKASRYGK----ISVEIERIFRRLVAVLQSVIRPAARALLDIHLNDHDATGD---------------VIHLLDTTMRQARICGLNAALTIQLCGHLLHMVNAFVFNSLVSVNLPAAQLNTRLGKCLQYRIESIHRFCERMGVEMAAECHLDRSRQAANLLATQKNDVAALGSTCYKL---NSLQVVHILSG--FEPDDGEIPCDDDVVDRICRLAEKQADKLTLEDGQRVTLEESDELNLPFLIPQDGYFVEQ-FRTVPDGLWQYLQQLQHRRLC 898
BLAST of Afadin vs. Ensembl Celegans
Match: afd-1 (AFaDin (Actin filament binding protein) homolog [Source:UniProtKB/TrEMBL;Acc:X5M8U5]) HSP 1 Score: 108.612 bits (270), Expect = 2.639e-23 Identity = 100/332 (30.12%), Postives = 162/332 (48.80%), Query Frame = 1
Query: 121 LINQWNSSRLELFKISQPDKNLKFFGVMRFFFE-TEDKFHSKCLRVSSDLKVKDLLPILLEKFYVSDICSSKQDFRLFE--RSSSIDKLLDPEDFPLLIQLFWCKDDKEGVFVFKS---------NLKNKNSRRIR----------SQTRKDKIGSTTCLNSPTTRCLDKVCTDPPTKSTKQXXXXXXXXXXXXXXXRTISNPXXXXXXXXXXXXXXXXXQISKSGNLNGGGTLKIYGDSLNPLIPYKSLLLSIDDTAEFVVREILDKYGMENENENDFSLVSSVYKDQNTQKIEYEEI----LQNSDSPLRFLLQ--SNKNDGFIKFRVVK 1032
L+ QWN +RL+LF +S P ++L+ GVMRF+F+ +K +KC+RVSS + ++ L EKF S + L+E + KLLD E+ PL++QL W KDD+EG F+ K NL+ + + R + +K+ + + D + P + +RTISNPE++MR +R+ KLE +K + GG+LK+YG + P PY S+L I++ A+ ++ L+KYG+E+ +++DF LV D + EI + ++ PL + N +GF F +K
Sbjct: 11 LVQQWNENRLDLFHLSYPTEDLEVEGVMRFYFQDGGEKVLTKCVRVSSTATTRAVVDALSEKFLPDLKMLSNDTYSLWEVHENGEERKLLD-EEKPLVVQLQWHKDDREGRFLLKKDAAPPVSLLNLQEADQKNTRFAKKKDKEPKKKNQKNFVSVQVNGGREEAQSQDDIYKQVPFNT----------------FTRTISNPEAVMRKRREKKLE------TKLKEMMFGGSLKVYGGEIVPTRPYVSILAEINENADRILGAALEKYGLEH-SKDDFILVEVSNDDDRKSMSDLREIDGRPIPPTECPLFEMTARSGNGENGFDSFLAIK 318 HSP 2 Score: 78.5666 bits (192), Expect = 3.085e-14 Identity = 78/296 (26.35%), Postives = 146/296 (49.32%), Query Frame = 1
Query: 1834 YWLANCTEFLHLLKNNILLSQFNSSIFDKYYDLFADNAQWCFQCISFGFTDDMVNTLSVLLNENTDDSEEKDDFPLSRKASDPKNMSILIRTLSSCMSVLQQCHVNPALIIQIISRIFHLINCWIFNSFIDT---PCRIFRGWAXXXXXXXXXXXXWSERHDLLLAADCYFQQSLQACKLLIMEKTDIEVFYNETVDMVDINSIQLRYLLDNYIYSPNEPKVP--QIWIDFVVSGANQVADKVFEEEGIDICXXXXXXXXXXXXXXXDGYHVDSLYGGVPVGFIEYLEKL-HKEWC 2703
+WLAN +EF HL++ + ++ +S + K + + F+ + + LL+ + +D + D +I L + M + C +N AL IQ+ + H++N ++FNS + ++ + + RIE I R+ ER + +AA+C+ +S QA LL +K D+ + + NS+Q+ ++L + P++ ++P +D + A + ADK+ E+G + L+ + LNLP L+P+DGY V+ + VP G +YL++L H+ C
Sbjct: 633 FWLANASEFNHLVERD---TELKASRYGK----ISVEIERIFRRLVAVLQSVIRPAARALLDIHLNDHDATGD---------------VIHLLDTTMRQARICGLNAALTIQLCGHLLHMVNAFVFNSLVSVNLPAAQLNTRLGKCLQYRIESIHRFCERMGVEMAAECHLDRSRQAANLLATQKNDVAALGSTCYKL---NSLQVVHILSG--FEPDDGEIPCDDDVVDRICRLAEKQADKLTLEDGQRVTLEESDELNLPFLIPQDGYFVEQ-FRTVPDGLWQYLQQLQHRRLC 900
BLAST of Afadin vs. Ensembl Celegans
Match: afd-1 (AFaDin (Actin filament binding protein) homolog [Source:UniProtKB/TrEMBL;Acc:X5M8U5]) HSP 1 Score: 74.3294 bits (181), Expect = 5.997e-13 Identity = 78/296 (26.35%), Postives = 142/296 (47.97%), Query Frame = 1
Query: 1834 YWLANCTEFLHLLKNNILLSQFNSSIFDKYYDLFADNAQWCFQCISFGFTDDMVNTLSVLLNENTDDSEEKDDFPLSRKASDPKNMSILIRTLSSCMSVLQQCHVNPALIIQIISRIFHLINCWIFNSFID---TPCRIFRGWAXXXXXXXXXXXXWSERHDLLLAADCYFQQSLQACKLLIMEKTDIEVFYNETVDMVDINSIQLRYLLDNYIYSPNEPKVP--QIWIDFVVSGANQVADKVFEEEGIDICXXXXXXXXXXXXXXXDGYHVDSLYGGVPVGFIEYLEKL-HKEWC 2703
+WLAN +EF HL++ + L Y + + F+ + + LL+ + +D + D +I L + M + C +N AL IQ+ + H++N ++FNS + ++ + + RIE I R+ ER + +AA+C+ +S QA LL +K D+ + + NS+Q+ ++L + P++ ++P +D + A + ADK+ E+G + L+ + LNLP L+P+DGY V+ + VP G +YL++L H+ C
Sbjct: 200 FWLANASEFNHLVERDTELKA-------SRYGKISVEIERIFRRLVAVLQSVIRPAARALLDIHLNDHDATGD---------------VIHLLDTTMRQARICGLNAALTIQLCGHLLHMVNAFVFNSLVSVNLPAAQLNTRLGKCLQYRIESIHRFCERMGVEMAAECHLDRSRQAANLLATQKNDVAALGSTCYKL---NSLQVVHILSG--FEPDDGEIPCDDDVVDRICRLAEKQADKLTLEDGQRVTLEESDELNLPFLIPQDGYFVEQ-FRTVPDGLWQYLQQLQHRRLC 467
BLAST of Afadin vs. Ensembl Fly
Match: cno (gene:FBgn0259212 transcript:FBtr0334911) HSP 1 Score: 268.47 bits (685), Expect = 2.329e-72 Identity = 249/911 (27.33%), Postives = 432/911 (47.42%), Query Frame = 1
Query: 121 LINQWNSSRLELFKISQPDKNLKFFGVMRFFFE-TEDKFHSKCLRVSSDLKVKDLLPILLEKFYVSDICSSKQDFRLFE-RSSSIDKLLDPEDFPLLIQLFWCKDDKEGVFVFKSNLKNKNSRRIRSQTR-KDKIGSTTCLNSPTTRCLDKVCTDPPTK------------------------------STKQXXXXXXXXXXXXXXXRTISNPXXXXXXXXXXXXXXXXXQI-SKSGNLNGGGTLKIYGDSLNPLIPYKSLLLSIDDTAEFVVREILDKYGMENENENDFSLVSSVYKDQNTQKIEYEEILQNSDSPLRFLLQSNKNDGFIKFRVVKMPANISKSKKSKTQGTFPDVCKSIAIRKSNSVNY----RNSDVQSHSRNTCDGKFKHSLHPDFSNNYNGSNNKIYSTPPSSDSPVPLLKRVESEKKPLPTYEESVSALRSWRVKISNFPENHKPISYRGSTESFSLSSDYQ-----EDSSYNTDSYESRNIPSV---ETPEQENDDVVIISDLDSHLSRH----LPVQITYFKSKEDMLLDSILLDIKPSQTKFKLASAYLLHLMVVDIIRLTCINRATVLDQELV--IGMFIEKVASKIRQSIMQHRTIISIQSYWLANCTEFLHLLKNNILLSQFNSSIFDKYYDLFADNAQWCFQCISFGFTDDMVNTLSVLLNENTDDSEEKDDFPLSRKASDPKNMSILIRTLSSCMSVLQQCHVNPALIIQIISRIFHLINCWIFNSFIDTPCRIFRGWAXXXXXXXXXXXXWSERHDLLLAADCYFQQSLQACKLLIMEKTDIEVFYNETVDMVDINSIQLRYLLDNYIYSPNEPKVPQIWIDFVVSGANQVADKVFEEEGIDICXXXXXXXXXXXXXXXDGYHVDSLYGGVPVGFIEYLEKLHKE 2697
+I QWN++RL+LF +S+PD+NL F GVMRF+F+ K +KC+RV+SD V D++ L+EKF S ++ L+E ++ ++ L+ ++ PLL+QL W DD+EG F+ K N+ K + ++ K K+ + K+ +DPPT S+ + + +R+ISNPE++MR +R+ KLE+KL+Q S+ G + GGTLKIYG+SL +PYK+LLLSI D A+ VVRE+L KYG+E + + LV N+ EY IL + + PL L+ + G I F V + PA+ ++ K + +N N+ R V ++ DG + L D + + N + PS L+ +E P + ++++ + + + I + GS F + Y+ D YN +S+ + +P+ ++ S ++ LP + + ++ +++ L I+ ++ + FKLA Y L+L R + R + E + MF+ VA+ + + + T I ++W+AN +EFLH LK++ +S F+ + ++ A+ Q F+ + F ++ TL+ L+EN D +++ L S M++L++C VN AL IQ+ S++FH IN FN+ + W +++ R++ + W+ER L LAADC+ + Q + L K+ +E +NS+Q+ LL + K+P+ +D + A VAD++ +G ++ L+ L+L LLLP+DG+ D + G+P G +++L L ++
Sbjct: 16 VIQQWNANRLDLFALSEPDENLLFHGVMRFYFQDAGQKVATKCIRVASDATVTDVIDTLIEKFRPDMRMLSVPNYALYEVHANGEERRLNADEKPLLVQLNWHIDDREGRFLLK-NIDQKTTPIEQTDLNFKRKLSKREKKEQKKKEKMAKLSSDPPTSNGSHLGLGNGVGASSGSAHMNGNAGGTGGDSSDAVAGKLYTELPETSFTRSISNPEAVMRRRRQQKLEKKLQQFRSRDGGPDTGGTLKIYGESLCQDVPYKTLLLSIRDCAQAVVREMLTKYGLEKADPLHYCLVQV-----NSDGTEY--ILDDDECPLSILMNHPTSRGSIMFHVRRRPADSQPRRRKKK-----------PLGAANGANHISGDREGPVLVEVTHSGDGGRRIKLGSDPVEVGSANTNCLQLFGPSIQPRHCLISLLEGVCTVTPLHTDALTFVNGHHI-------SQPTILHNGSVVMFGRVASYRFLDSPTDGRYNLALSQSQLDSACLYERSPKMTGSGILAGGGSGSGDTQGQEPILPAVLEFPETHQELFLRHIISELDVNVPHFKLAPVYSLYLCAR--YRASTHYRPELQPTERAHKLTMFLHHVANLVYSVVQEQYTDPRILAFWMANSSEFLHFLKSDRHISAFSV----QAQEVLAEAVQTAFRNLVNCFRLELSQTLNQFLSENIDHDSAA---------------GLVLTVLGSAMALLRRCRVNAALTIQLFSQLFHYINVICFNTIVANSQMCTGDWGKVMTERLQLLELWAERQGLELAADCHLAKINQCAQFLQAPKSSVEEIQQLACSCFRLNSLQMAALLQ-------QEKLPRNLVDTAIRMAESVADELTRADGREVRLEESPELHLALLLPDDGFSCDVVR-GIPTGLVDFLNPLQQQ 871
BLAST of Afadin vs. Ensembl Fly
Match: cno (gene:FBgn0259212 transcript:FBtr0334912) HSP 1 Score: 159.073 bits (401), Expect = 1.349e-38 Identity = 122/350 (34.86%), Postives = 188/350 (53.71%), Query Frame = 1
Query: 121 LINQWNSSRLELFKISQPDKNLKFFGVMRFFFE-TEDKFHSKCLRVSSDLKVKDLLPILLEKFYVSDICSSKQDFRLFE-RSSSIDKLLDPEDFPLLIQLFWCKDDKEGVFVFKSNLKNKNSRRIRSQTR-KDKIGSTTCLNSPTTRCLDKVCTDPPTK------------------------------STKQXXXXXXXXXXXXXXXRTISNPXXXXXXXXXXXXXXXXXQI-SKSGNLNGGGTLKIYGDSLNPLIPYKSLLLSIDDTAEFVVREILDKYGMENENENDFSLVSSVYKDQNTQKIEYEEILQNSDSPLRFLLQSNKNDGFIKFRVVKMPANISKSKKSK 1068
+I QWN++RL+LF +S+PD+NL F GVMRF+F+ K +KC+RV+SD V D++ L+EKF S ++ L+E ++ ++ L+ ++ PLL+QL W DD+EG F+ K N+ K + ++ K K+ + K+ +DPPT S+ + + +R+ISNPE++MR +R+ KLE+KL+Q S+ G + GGTLKIYG+SL +PYK+LLLSI D A+ VVRE+L KYG+E + + LV N+ EY IL + + PL L+ + G I F V + PA+ ++ K
Sbjct: 16 VIQQWNANRLDLFALSEPDENLLFHGVMRFYFQDAGQKVATKCIRVASDATVTDVIDTLIEKFRPDMRMLSVPNYALYEVHANGEERRLNADEKPLLVQLNWHIDDREGRFLLK-NIDQKTTPIEQTDLNFKRKLSKREKKEQKKKEKMAKLSSDPPTSNGSHLGLGNGVGASSGSAHMNGNAGGTGGDSSDAVAGKLYTELPETSFTRSISNPEAVMRRRRQQKLEKKLQQFRSRDGGPDTGGTLKIYGESLCQDVPYKTLLLSIRDCAQAVVREMLTKYGLEKADPLHYCLVQV-----NSDGTEY--ILDDDECPLSILMNHPTSRGSIMFHVRRRPADSQPRRRKK 357 HSP 2 Score: 136.346 bits (342), Expect = 1.027e-31 Identity = 102/379 (26.91%), Postives = 188/379 (49.60%), Query Frame = 1
Query: 1567 LPVQITYFKSKEDMLLDSILLDIKPSQTKFKLASAYLLHLMVVDIIRLTCINRATVLDQELV--IGMFIEKVASKIRQSIMQHRTIISIQSYWLANCTEFLHLLKNNILLSQFNSSIFDKYYDLFADNAQWCFQCISFGFTDDMVNTLSVLLNENTDDSEEKDDFPLSRKASDPKNMSILIRTLSSCMSVLQQCHVNPALIIQIISRIFHLINCWIFNSFIDTPCRIFRGWAXXXXXXXXXXXXWSERHDLLLAADCYFQQSLQACKLLIMEKTDIEVFYNETVDMVDINSIQLRYLLDNYIYSPNEPKVPQIWIDFVVSGANQVADKVFEEEGIDICXXXXXXXXXXXXXXXDGYHVDSLYGGVPVGFIEYLEKLHKE 2697
LP + + ++ +++ L I+ ++ + FKLA Y L+L R + R + E + MF+ VA+ + + + T I ++W+AN +EFLH LK++ +S F+ + ++ A+ Q F+ + F ++ TL+ L+EN D +++ L S M++L++C VN AL IQ+ S++FH IN FN+ + W +++ R++ + W+ER L LAADC+ + Q + L K+ +E +NS+Q+ LL + K+P+ +D + A VAD++ +G ++ L+ L+L LLLP+DG+ D + G+P G +++L L ++
Sbjct: 622 LPAVLEFPETHQELFLRHIISELDVNVPHFKLAPVYSLYLCAR--YRASTHYRPELQPTERAHKLTMFLHHVANLVYSVVQEQYTDPRILAFWMANSSEFLHFLKSDRHISAFSV----QAQEVLAEAVQTAFRNLVNCFRLELSQTLNQFLSENIDHDSAA---------------GLVLTVLGSAMALLRRCRVNAALTIQLFSQLFHYINVICFNTIVANSQMCTGDWGKVMTERLQLLELWAERQGLELAADCHLAKINQCAQFLQAPKSSVEEIQQLACSCFRLNSLQMAALL-------QQEKLPRNLVDTAIRMAESVADELTRADGREVRLEESPELHLALLLPDDGFSCDVVR-GIPTGLVDFLNPLQQQ 971
BLAST of Afadin vs. Ensembl Fly
Match: cno (gene:FBgn0259212 transcript:FBtr0299710) HSP 1 Score: 158.688 bits (400), Expect = 1.536e-38 Identity = 122/354 (34.46%), Postives = 189/354 (53.39%), Query Frame = 1
Query: 109 TILHLINQWNSSRLELFKISQPDKNLKFFGVMRFFFE-TEDKFHSKCLRVSSDLKVKDLLPILLEKFYVSDICSSKQDFRLFE-RSSSIDKLLDPEDFPLLIQLFWCKDDKEGVFVFKSNLKNKNSRRIRSQTR-KDKIGSTTCLNSPTTRCLDKVCTDPPTK------------------------------STKQXXXXXXXXXXXXXXXRTISNPXXXXXXXXXXXXXXXXXQI-SKSGNLNGGGTLKIYGDSLNPLIPYKSLLLSIDDTAEFVVREILDKYGMENENENDFSLVSSVYKDQNTQKIEYEEILQNSDSPLRFLLQSNKNDGFIKFRVVKMPANISKSKKSK 1068
+ +I QWN++RL+LF +S+PD+NL F GVMRF+F+ K +KC+RV+SD V D++ L+EKF S ++ L+E ++ ++ L+ ++ PLL+QL W DD+EG F+ K N+ K + ++ K K+ + K+ +DPPT S+ + + +R+ISNPE++MR +R+ KLE+KL+Q S+ G + GGTLKIYG+SL +PYK+LLLSI D A+ VVRE+L KYG+E + + LV N+ EY IL + + PL L+ + G I F V + PA+ ++ K
Sbjct: 12 AVRSVIQQWNANRLDLFALSEPDENLLFHGVMRFYFQDAGQKVATKCIRVASDATVTDVIDTLIEKFRPDMRMLSVPNYALYEVHANGEERRLNADEKPLLVQLNWHIDDREGRFLLK-NIDQKTTPIEQTDLNFKRKLSKREKKEQKKKEKMAKLSSDPPTSNGSHLGLGNGVGASSGSAHMNGNAGGTGGDSSDAVAGKLYTELPETSFTRSISNPEAVMRRRRQQKLEKKLQQFRSRDGGPDTGGTLKIYGESLCQDVPYKTLLLSIRDCAQAVVREMLTKYGLEKADPLHYCLVQV-----NSDGTEY--ILDDDECPLSILMNHPTSRGSIMFHVRRRPADSQPRRRKK 357 HSP 2 Score: 137.502 bits (345), Expect = 4.349e-32 Identity = 102/379 (26.91%), Postives = 188/379 (49.60%), Query Frame = 1
Query: 1567 LPVQITYFKSKEDMLLDSILLDIKPSQTKFKLASAYLLHLMVVDIIRLTCINRATVLDQELV--IGMFIEKVASKIRQSIMQHRTIISIQSYWLANCTEFLHLLKNNILLSQFNSSIFDKYYDLFADNAQWCFQCISFGFTDDMVNTLSVLLNENTDDSEEKDDFPLSRKASDPKNMSILIRTLSSCMSVLQQCHVNPALIIQIISRIFHLINCWIFNSFIDTPCRIFRGWAXXXXXXXXXXXXWSERHDLLLAADCYFQQSLQACKLLIMEKTDIEVFYNETVDMVDINSIQLRYLLDNYIYSPNEPKVPQIWIDFVVSGANQVADKVFEEEGIDICXXXXXXXXXXXXXXXDGYHVDSLYGGVPVGFIEYLEKLHKE 2697
LP + + ++ +++ L I+ ++ + FKLA Y L+L R + R + E + MF+ VA+ + + + T I ++W+AN +EFLH LK++ +S F+ + ++ A+ Q F+ + F ++ TL+ L+EN D +++ L S M++L++C VN AL IQ+ S++FH IN FN+ + W +++ R++ + W+ER L LAADC+ + Q + L K+ +E +NS+Q+ LL + K+P+ +D + A VAD++ +G ++ L+ L+L LLLP+DG+ D + G+P G +++L L ++
Sbjct: 622 LPAVLEFPETHQELFLRHIISELDVNVPHFKLAPVYSLYLCAR--YRASTHYRPELQPTERAHKLTMFLHHVANLVYSVVQEQYTDPRILAFWMANSSEFLHFLKSDRHISAFSV----QAQEVLAEAVQTAFRNLVNCFRLELSQTLNQFLSENIDHDSA---------------AGLVLTVLGSAMALLRRCRVNAALTIQLFSQLFHYINVICFNTIVANSQMCTGDWGKVMTERLQLLELWAERQGLELAADCHLAKINQCAQFLQAPKSSVEEIQQLACSCFRLNSLQMAALL-------QQEKLPRNLVDTAIRMAESVADELTRADGREVRLEESPELHLALLLPDDGFSCDVVR-GIPTGLVDFLNPLQQQ 971
BLAST of Afadin vs. Ensembl Fly
Match: cno (gene:FBgn0259212 transcript:FBtr0334913) HSP 1 Score: 158.688 bits (400), Expect = 1.590e-38 Identity = 122/354 (34.46%), Postives = 189/354 (53.39%), Query Frame = 1
Query: 109 TILHLINQWNSSRLELFKISQPDKNLKFFGVMRFFFE-TEDKFHSKCLRVSSDLKVKDLLPILLEKFYVSDICSSKQDFRLFE-RSSSIDKLLDPEDFPLLIQLFWCKDDKEGVFVFKSNLKNKNSRRIRSQTR-KDKIGSTTCLNSPTTRCLDKVCTDPPTK------------------------------STKQXXXXXXXXXXXXXXXRTISNPXXXXXXXXXXXXXXXXXQI-SKSGNLNGGGTLKIYGDSLNPLIPYKSLLLSIDDTAEFVVREILDKYGMENENENDFSLVSSVYKDQNTQKIEYEEILQNSDSPLRFLLQSNKNDGFIKFRVVKMPANISKSKKSK 1068
+ +I QWN++RL+LF +S+PD+NL F GVMRF+F+ K +KC+RV+SD V D++ L+EKF S ++ L+E ++ ++ L+ ++ PLL+QL W DD+EG F+ K N+ K + ++ K K+ + K+ +DPPT S+ + + +R+ISNPE++MR +R+ KLE+KL+Q S+ G + GGTLKIYG+SL +PYK+LLLSI D A+ VVRE+L KYG+E + + LV N+ EY IL + + PL L+ + G I F V + PA+ ++ K
Sbjct: 12 AVRSVIQQWNANRLDLFALSEPDENLLFHGVMRFYFQDAGQKVATKCIRVASDATVTDVIDTLIEKFRPDMRMLSVPNYALYEVHANGEERRLNADEKPLLVQLNWHIDDREGRFLLK-NIDQKTTPIEQTDLNFKRKLSKREKKEQKKKEKMAKLSSDPPTSNGSHLGLGNGVGASSGSAHMNGNAGGTGGDSSDAVAGKLYTELPETSFTRSISNPEAVMRRRRQQKLEKKLQQFRSRDGGPDTGGTLKIYGESLCQDVPYKTLLLSIRDCAQAVVREMLTKYGLEKADPLHYCLVQV-----NSDGTEY--ILDDDECPLSILMNHPTSRGSIMFHVRRRPADSQPRRRKK 357 HSP 2 Score: 137.502 bits (345), Expect = 4.655e-32 Identity = 102/379 (26.91%), Postives = 188/379 (49.60%), Query Frame = 1
Query: 1567 LPVQITYFKSKEDMLLDSILLDIKPSQTKFKLASAYLLHLMVVDIIRLTCINRATVLDQELV--IGMFIEKVASKIRQSIMQHRTIISIQSYWLANCTEFLHLLKNNILLSQFNSSIFDKYYDLFADNAQWCFQCISFGFTDDMVNTLSVLLNENTDDSEEKDDFPLSRKASDPKNMSILIRTLSSCMSVLQQCHVNPALIIQIISRIFHLINCWIFNSFIDTPCRIFRGWAXXXXXXXXXXXXWSERHDLLLAADCYFQQSLQACKLLIMEKTDIEVFYNETVDMVDINSIQLRYLLDNYIYSPNEPKVPQIWIDFVVSGANQVADKVFEEEGIDICXXXXXXXXXXXXXXXDGYHVDSLYGGVPVGFIEYLEKLHKE 2697
LP + + ++ +++ L I+ ++ + FKLA Y L+L R + R + E + MF+ VA+ + + + T I ++W+AN +EFLH LK++ +S F+ + ++ A+ Q F+ + F ++ TL+ L+EN D +++ L S M++L++C VN AL IQ+ S++FH IN FN+ + W +++ R++ + W+ER L LAADC+ + Q + L K+ +E +NS+Q+ LL + K+P+ +D + A VAD++ +G ++ L+ L+L LLLP+DG+ D + G+P G +++L L ++
Sbjct: 622 LPAVLEFPETHQELFLRHIISELDVNVPHFKLAPVYSLYLCAR--YRASTHYRPELQPTERAHKLTMFLHHVANLVYSVVQEQYTDPRILAFWMANSSEFLHFLKSDRHISAFSV----QAQEVLAEAVQTAFRNLVNCFRLELSQTLNQFLSENIDHDSA---------------AGLVLTVLGSAMALLRRCRVNAALTIQLFSQLFHYINVICFNTIVANSQMCTGDWGKVMTERLQLLELWAERQGLELAADCHLAKINQCAQFLQAPKSSVEEIQQLACSCFRLNSLQMAALL-------QQEKLPRNLVDTAIRMAESVADELTRADGREVRLEESPELHLALLLPDDGFSCDVVR-GIPTGLVDFLNPLQQQ 971
BLAST of Afadin vs. Ensembl Fly
Match: cno (gene:FBgn0259212 transcript:FBtr0300525) HSP 1 Score: 158.688 bits (400), Expect = 1.621e-38 Identity = 122/350 (34.86%), Postives = 188/350 (53.71%), Query Frame = 1
Query: 121 LINQWNSSRLELFKISQPDKNLKFFGVMRFFFE-TEDKFHSKCLRVSSDLKVKDLLPILLEKFYVSDICSSKQDFRLFE-RSSSIDKLLDPEDFPLLIQLFWCKDDKEGVFVFKSNLKNKNSRRIRSQTR-KDKIGSTTCLNSPTTRCLDKVCTDPPTK------------------------------STKQXXXXXXXXXXXXXXXRTISNPXXXXXXXXXXXXXXXXXQI-SKSGNLNGGGTLKIYGDSLNPLIPYKSLLLSIDDTAEFVVREILDKYGMENENENDFSLVSSVYKDQNTQKIEYEEILQNSDSPLRFLLQSNKNDGFIKFRVVKMPANISKSKKSK 1068
+I QWN++RL+LF +S+PD+NL F GVMRF+F+ K +KC+RV+SD V D++ L+EKF S ++ L+E ++ ++ L+ ++ PLL+QL W DD+EG F+ K N+ K + ++ K K+ + K+ +DPPT S+ + + +R+ISNPE++MR +R+ KLE+KL+Q S+ G + GGTLKIYG+SL +PYK+LLLSI D A+ VVRE+L KYG+E + + LV N+ EY IL + + PL L+ + G I F V + PA+ ++ K
Sbjct: 16 VIQQWNANRLDLFALSEPDENLLFHGVMRFYFQDAGQKVATKCIRVASDATVTDVIDTLIEKFRPDMRMLSVPNYALYEVHANGEERRLNADEKPLLVQLNWHIDDREGRFLLK-NIDQKTTPIEQTDLNFKRKLSKREKKEQKKKEKMAKLSSDPPTSNGSHLGLGNGVGASSGSAHMNGNAGGTGGDSSDAVAGKLYTELPETSFTRSISNPEAVMRRRRQQKLEKKLQQFRSRDGGPDTGGTLKIYGESLCQDVPYKTLLLSIRDCAQAVVREMLTKYGLEKADPLHYCLVQV-----NSDGTEY--ILDDDECPLSILMNHPTSRGSIMFHVRRRPADSQPRRRKK 357 HSP 2 Score: 136.732 bits (343), Expect = 9.604e-32 Identity = 102/379 (26.91%), Postives = 188/379 (49.60%), Query Frame = 1
Query: 1567 LPVQITYFKSKEDMLLDSILLDIKPSQTKFKLASAYLLHLMVVDIIRLTCINRATVLDQELV--IGMFIEKVASKIRQSIMQHRTIISIQSYWLANCTEFLHLLKNNILLSQFNSSIFDKYYDLFADNAQWCFQCISFGFTDDMVNTLSVLLNENTDDSEEKDDFPLSRKASDPKNMSILIRTLSSCMSVLQQCHVNPALIIQIISRIFHLINCWIFNSFIDTPCRIFRGWAXXXXXXXXXXXXWSERHDLLLAADCYFQQSLQACKLLIMEKTDIEVFYNETVDMVDINSIQLRYLLDNYIYSPNEPKVPQIWIDFVVSGANQVADKVFEEEGIDICXXXXXXXXXXXXXXXDGYHVDSLYGGVPVGFIEYLEKLHKE 2697
LP + + ++ +++ L I+ ++ + FKLA Y L+L R + R + E + MF+ VA+ + + + T I ++W+AN +EFLH LK++ +S F+ + ++ A+ Q F+ + F ++ TL+ L+EN D +++ L S M++L++C VN AL IQ+ S++FH IN FN+ + W +++ R++ + W+ER L LAADC+ + Q + L K+ +E +NS+Q+ LL + K+P+ +D + A VAD++ +G ++ L+ L+L LLLP+DG+ D + G+P G +++L L ++
Sbjct: 622 LPAVLEFPETHQELFLRHIISELDVNVPHFKLAPVYSLYLCAR--YRASTHYRPELQPTERAHKLTMFLHHVANLVYSVVQEQYTDPRILAFWMANSSEFLHFLKSDRHISAFSV----QAQEVLAEAVQTAFRNLVNCFRLELSQTLNQFLSENIDHDSAA---------------GLVLTVLGSAMALLRRCRVNAALTIQLFSQLFHYINVICFNTIVANSQMCTGDWGKVMTERLQLLELWAERQGLELAADCHLAKINQCAQFLQAPKSSVEEIQQLACSCFRLNSLQMAALLQ-------QEKLPRNLVDTAIRMAESVADELTRADGREVRLEESPELHLALLLPDDGFSCDVVR-GIPTGLVDFLNPLQQQ 971
BLAST of Afadin vs. Ensembl Zebrafish
Match: afdna (afadin, adherens junction formation factor a [Source:ZFIN;Acc:ZDB-GENE-050419-52]) HSP 1 Score: 159.844 bits (403), Expect = 8.829e-39 Identity = 119/354 (33.62%), Postives = 181/354 (51.13%), Query Frame = 1
Query: 121 LINQWNSSRLELFKISQPDKNLKFFGVMRFFFE--TEDKFHSKCLRVSSDLKVKDLLPILLEKFYVSDICSSKQDFRLFE-RSSSIDKLLDPEDFPLLIQLFWCKDDKEGVFVFKSNLKNKNSRRIRSQTRKDKI-------------------------------GSTTCLN---SPTTRCLDKVCTDPPTKSTKQXXXXXXXXXXXXXXXRTISNPXXXXXXXXXXXXXXXXXQI-SKSGNLNGGGTLKIYGDSLNPLIPYKSLLLSIDDTAEFVVREILDKYGMENENENDFSLVSSVYKDQNTQKIEYEEILQNSDSPLRFLLQSNKNDGFIKFRVVKMPANISKSKKSK 1068
+IN WN++RL+LF+ISQP ++L+F GVMRF+F+ F +KC+RVSS +D++ L EKF S + L+E S ++ LD ++ PL++QL W KDD+EG FV K+ R + + K+K G LN +R +V D P S +RTISNPE +M+ +R+ KLE+++++ S+ G + GGTL+IY DSL P IPYK++LLS D A+F V E L+KYG+E EN D+ + ++ + K E IL ++D PL+ L + + F++ K P + + K K
Sbjct: 95 IINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERKLDLDEKPLVVQLNWNKDDREGRFVLKNENDIMPKRTLTNGPEKEKEGVIQNFKRTLSKKEKKKEKKREKEAFSRIPDGDDHVLNREDGENSRLAAEVYKDMPETS----------------FTRTISNPEVVMKRRRQQKLEKRMQEFRSEDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDMADFAVAEALEKYGLEKENPRDYCIAQVIHANDKAVK---ETILDDTDCPLQILRDWTSDKEALVFQLKKRPPDYAPRKGRK 429 HSP 2 Score: 157.532 bits (397), Expect = 4.573e-38 Identity = 115/378 (30.42%), Postives = 191/378 (50.53%), Query Frame = 1
Query: 1567 LPVQITYFKSKEDMLLDSILLDIKPSQTKFKLASAYLLHLMVVDIIRLTCINRATVLDQELVIGMFIEKVASKIRQSIMQHRTIISIQSYWLANCTEFLHLLKNNILLSQFNSSIFDKYYDLFADNAQWCFQCISFGFTDDMVNTLSVLLNENTDDSEEKDDFPLSRKASDPKNMSILIRTLSSCMSVLQQCHVNPALIIQIISRIFHLINCWIFNSFIDTP----CRIFRGWAXXXXXXXXXXXXWSERHDLLLAADCYFQQSLQACKLLIMEKTDIEVFYNETVDMVDINSIQLRYLLDNYIYSPNEPKVPQIWIDFVVSGANQVADKVFEEEGIDICXXXXXXXXXXXXXXXDGYHVDSLYGGVPVGFIEYLEKL 2688
LP I + ++ ED L +I+ S FKL+ Y+L++ ++ + ++ + + K+ S + I + + I ++W+AN +E L+ +K + LS+ I D+ A Q F+ + D+ N + L +D +E++ P K D ++ TL+ MS+L++C VN AL IQ+ S++FH +N W+FN + C + W I+ ++ I W+E+ L LAADC+ + +QA LL M+K ++ N +NS+QL LL NY +P+EP +P ID VV+ A AD++ +G ++ L+ L LP LLPEDGY D + +P GF E+LE L
Sbjct: 653 LPASIEFRENSEDAFLSAIINYTNSSTVHFKLSPTYVLYMTCRYVMSSQYRPDISPSERTHKVIAIVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDKDLSR----ITLDAQDVLAHLVQMAFKYLVHCLQSDLNNYMPAFL----EDPDEQN--PQRPKIED------VLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFVNMWLFNKLVTEADSGLCSHY--WGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVQNINNTCFKLNSLQLNALLSNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVR-NIPNGFQEFLEPL 1011
BLAST of Afadin vs. Ensembl Zebrafish
Match: afdna (afadin, adherens junction formation factor a [Source:ZFIN;Acc:ZDB-GENE-050419-52]) HSP 1 Score: 159.458 bits (402), Expect = 1.195e-38 Identity = 119/354 (33.62%), Postives = 181/354 (51.13%), Query Frame = 1
Query: 121 LINQWNSSRLELFKISQPDKNLKFFGVMRFFFE--TEDKFHSKCLRVSSDLKVKDLLPILLEKFYVSDICSSKQDFRLFE-RSSSIDKLLDPEDFPLLIQLFWCKDDKEGVFVFKSNLKNKNSRRIRSQTRKDKI-------------------------------GSTTCLN---SPTTRCLDKVCTDPPTKSTKQXXXXXXXXXXXXXXXRTISNPXXXXXXXXXXXXXXXXXQI-SKSGNLNGGGTLKIYGDSLNPLIPYKSLLLSIDDTAEFVVREILDKYGMENENENDFSLVSSVYKDQNTQKIEYEEILQNSDSPLRFLLQSNKNDGFIKFRVVKMPANISKSKKSK 1068
+IN WN++RL+LF+ISQP ++L+F GVMRF+F+ F +KC+RVSS +D++ L EKF S + L+E S ++ LD ++ PL++QL W KDD+EG FV K+ R + + K+K G LN +R +V D P S +RTISNPE +M+ +R+ KLE+++++ S+ G + GGTL+IY DSL P IPYK++LLS D A+F V E L+KYG+E EN D+ + ++ + K E IL ++D PL+ L + + F++ K P + + K K
Sbjct: 79 IINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERKLDLDEKPLVVQLNWNKDDREGRFVLKNENDIMPKRTLTNGPEKEKEGVIQNFKRTLSKKEKKKEKKREKEAFSRIPDGDDHVLNREDGENSRLAAEVYKDMPETS----------------FTRTISNPEVVMKRRRQQKLEKRMQEFRSEDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDMADFAVAEALEKYGLEKENPRDYCIAQVIHANDKAVK---ETILDDTDCPLQILRDWTSDKEALVFQLKKRPPDYAPRKGRK 413 HSP 2 Score: 157.918 bits (398), Expect = 3.628e-38 Identity = 115/378 (30.42%), Postives = 191/378 (50.53%), Query Frame = 1
Query: 1567 LPVQITYFKSKEDMLLDSILLDIKPSQTKFKLASAYLLHLMVVDIIRLTCINRATVLDQELVIGMFIEKVASKIRQSIMQHRTIISIQSYWLANCTEFLHLLKNNILLSQFNSSIFDKYYDLFADNAQWCFQCISFGFTDDMVNTLSVLLNENTDDSEEKDDFPLSRKASDPKNMSILIRTLSSCMSVLQQCHVNPALIIQIISRIFHLINCWIFNSFIDTP----CRIFRGWAXXXXXXXXXXXXWSERHDLLLAADCYFQQSLQACKLLIMEKTDIEVFYNETVDMVDINSIQLRYLLDNYIYSPNEPKVPQIWIDFVVSGANQVADKVFEEEGIDICXXXXXXXXXXXXXXXDGYHVDSLYGGVPVGFIEYLEKL 2688
LP I + ++ ED L +I+ S FKL+ Y+L++ ++ + ++ + + K+ S + I + + I ++W+AN +E L+ +K + LS+ I D+ A Q F+ + D+ N + L +D +E++ P K D ++ TL+ MS+L++C VN AL IQ+ S++FH +N W+FN + C + W I+ ++ I W+E+ L LAADC+ + +QA LL M+K ++ N +NS+QL LL NY +P+EP +P ID VV+ A AD++ +G ++ L+ L LP LLPEDGY D + +P GF E+LE L
Sbjct: 637 LPASIEFRENSEDAFLSAIINYTNSSTVHFKLSPTYVLYMTCRYVMSSQYRPDISPSERTHKVIAIVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDKDLSR----ITLDAQDVLAHLVQMAFKYLVHCLQSDLNNYMPAFL----EDPDEQN--PQRPKIED------VLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFVNMWLFNKLVTEADSGLCSHY--WGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSMQDVQNINNTCFKLNSLQLNALLSNYHCAPDEPYIPPELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVR-NIPNGFQEFLEPL 995
BLAST of Afadin vs. Ensembl Xenopus
Match: AFDN (afadin, adherens junction formation factor [Source:NCBI gene;Acc:100493083]) HSP 1 Score: 162.155 bits (409), Expect = 2.105e-39 Identity = 124/353 (35.13%), Postives = 187/353 (52.97%), Query Frame = 1
Query: 121 LINQWNSSRLELFKISQPDKNLKFFGVMRFFFE--TEDKFHSKCLRVSSDLKVKDLLPILLEKFYVSDICSSKQDFRLFERSSSIDKLLDPEDFPLLIQLFWCKDDKEGVFVFKSN-----LKNKNSRRIRSQTRKDKIGS-TTCLNSPTTRCLDKVCTDPPTKSTK--------------QXXXXXXXXXXXXXXXRTISNPXXXXXXXXXXXXXXXXXQISKS-GNLNGGGTLKIYGDSLNPLIPYKSLLLSIDDTAEFVVREILDKYGMENENENDFSLVSSVY--KDQNTQKIEYEEILQNSDSPLRFLLQSNKNDGFIKFRVVKMPANI---------SKSKKSKTQG 1077
+IN WN++RL+LF+ISQP ++L+F GVMRF+F+ F +KC+RVSS +D++ L EKF S + L+E S ++ LD E+ PL++QL W KDD+EG FV K+ K S Q ++ I + L+ + + D +ST Q + + +RTISNPE +M+ +R+ KLE+++++ S G + GGTL+IY DSL P IPYK++LLS +DTA+F V E L+KYGME EN ++ + V Q++ K E IL + + PL G + F++ + P + SKS KSK +G
Sbjct: 15 IINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPRYSLYEVHVSGERKLDFEEKPLVVQLNWNKDDREGRFVLKNENDIFPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKRRERDILKQSTDNEDRPQVRDDSENLQLAMEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTNDTADFAVIEALEKYGMEKENPKEYCIAKVVLPPGAQHSDKGMKEFILDDEECPLHIFRDWPSEKGELVFQLKRRPTDSLLKQNRKHESKSGKSKERG 367 HSP 2 Score: 156.377 bits (394), Expect = 1.224e-37 Identity = 116/378 (30.69%), Postives = 188/378 (49.74%), Query Frame = 1
Query: 1567 LPVQITYFKSKEDMLLDSILLDIKPSQTKFKLASAYLLHLMVVDIIRLTCINRATVLDQELVIGMFIEKVASKIRQSIMQHRTIISIQSYWLANCTEFLHLLKNNILLSQFNSSIFDKYYDLFADNAQWCFQCISFGFTDDMVNTLSVLLNENTDDSEEKDDFPLSRKASDPKNMSILIRTLSSCMSVLQQCHVNPALIIQIISRIFHLINCWIFNSFIDTP----CRIFRGWAXXXXXXXXXXXXWSERHDLLLAADCYFQQSLQACKLLIMEKTDIEVFYNETVDMVDINSIQLRYLLDNYIYSPNEPKVPQIWIDFVVSGANQVADKVFEEEGIDICXXXXXXXXXXXXXXXDGYHVDSLYGGVPVGFIEYLEKL 2688
LP I + ++ ED L +I+ S FKL+ Y+L++ ++ + ++ + + K+ + I + + I ++W+AN +E L+ +K + N D D+ A Q F+ + ++ +++ + DD EE D + PK +L TL+ MS+L++C VN AL IQ+ S++FH IN W+FN + P C + W II ++ I W+E+ L LAADC+ + +QA LL M+K + N +NS+QL+ LL NY +P+EP +P ID VVS A AD++ +G ++ L+ L LP LLPEDGY D + +P G E+LE L
Sbjct: 518 LPANIEFRENSEDAFLSAIINYTNSSTVHFKLSPTYVLYMTCRYVLSNKYRPDTSPAERTHKVIAVVNKMVGMMESVIQKQKNIAGALAFWMANASELLNFIKQD---RDLNRITLDAQ-DVLAHLVQMAFKYLVHCLQSELTSSMPAFI----DDPEEND-------SQRPKIEDVL-HTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHY--WGAIIRQQLAHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSHQDIPNINNTCFKLNSLQLQALLQNYHCAPDEPYIPTELIDSVVSVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVR-NIPNGLQEFLEPL 876
BLAST of Afadin vs. Ensembl Xenopus
Match: AFDN (afadin, adherens junction formation factor [Source:NCBI gene;Acc:100493083]) HSP 1 Score: 162.155 bits (409), Expect = 2.579e-39 Identity = 124/353 (35.13%), Postives = 187/353 (52.97%), Query Frame = 1
Query: 121 LINQWNSSRLELFKISQPDKNLKFFGVMRFFFE--TEDKFHSKCLRVSSDLKVKDLLPILLEKFYVSDICSSKQDFRLFERSSSIDKLLDPEDFPLLIQLFWCKDDKEGVFVFKSN-----LKNKNSRRIRSQTRKDKIGS-TTCLNSPTTRCLDKVCTDPPTKSTK--------------QXXXXXXXXXXXXXXXRTISNPXXXXXXXXXXXXXXXXXQISKS-GNLNGGGTLKIYGDSLNPLIPYKSLLLSIDDTAEFVVREILDKYGMENENENDFSLVSSVY--KDQNTQKIEYEEILQNSDSPLRFLLQSNKNDGFIKFRVVKMPANI---------SKSKKSKTQG 1077
+IN WN++RL+LF+ISQP ++L+F GVMRF+F+ F +KC+RVSS +D++ L EKF S + L+E S ++ LD E+ PL++QL W KDD+EG FV K+ K S Q ++ I + L+ + + D +ST Q + + +RTISNPE +M+ +R+ KLE+++++ S G + GGTL+IY DSL P IPYK++LLS +DTA+F V E L+KYGME EN ++ + V Q++ K E IL + + PL G + F++ + P + SKS KSK +G
Sbjct: 15 IINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPRYSLYEVHVSGERKLDFEEKPLVVQLNWNKDDREGRFVLKNENDIFPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKRRERDILKQSTDNEDRPQVRDDSENLQLAMEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTNDTADFAVIEALEKYGMEKENPKEYCIAKVVLPPGAQHSDKGMKEFILDDEECPLHIFRDWPSEKGELVFQLKRRPTDSLLKQNRKHESKSGKSKERG 367 HSP 2 Score: 146.747 bits (369), Expect = 1.249e-34 Identity = 116/383 (30.29%), Postives = 186/383 (48.56%), Query Frame = 1
Query: 1600 EDMLLDSILLDIKPSQTKFKLASAYLLHLMVVDIIR----------------LTCINRATVLDQELVIGMFIEKVASKIRQSIMQHRTIISIQSYWLANCTEFLHLLKNNILLSQFNSSIFDKYYDLFADNAQWCFQCISFGFTDDMVNTLSVLLNENTDDSEEKDDFPLSRKASDPKNMSILIRTLSSCMSVLQQCHVNPALIIQIISRIFHLINCWIFNSFIDTP----CRIFRGWAXXXXXXXXXXXXWSERHDLLLAADCYFQQSLQACKLLIMEKTDIEVFYNETVDMVDINSIQLRYLLDNYIYSPNEPKVPQIWIDFVVSGANQVADKVFEEEGIDICXXXXXXXXXXXXXXXDGYHVDSLYGGVPVGFIEYLEKL 2688
ED L +I+ S FKL+ Y+L++ ++ + +N+ ++GM +E V +I + + + I ++W+AN +E L+ +K + N D D+ A Q F+ + ++ +++ + DD EE D R D ++ TL+ MS+L++C VN AL IQ+ S++FH IN W+FN + P C + W II ++ I W+E+ L LAADC+ + +QA LL M+K + N +NS+QL+ LL NY +P+EP +P ID VVS A AD++ +G ++ L+ L LP LLPEDGY D + +P G E+LE L
Sbjct: 563 EDAFLSAIINYTNSSTVHFKLSPTYVLYMTCRYVLSNKYRPDTSPAERTHKVIAVVNK--------MVGM-MESVIQEIDKGNQKQKNIAGALAFWMANASELLNFIKQD---RDLNRITLDAQ-DVLAHLVQMAFKYLVHCLQSELTSSMPAFI----DDPEENDS---QRPKID------VLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHY--WGAIIRQQLAHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSHQDIPNINNTCFKLNSLQLQALLQNYHCAPDEPYIPTELIDSVVSVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVR-NIPNGLQEFLEPL 916
BLAST of Afadin vs. Ensembl Xenopus
Match: AFDN (afadin, adherens junction formation factor [Source:NCBI gene;Acc:100493083]) HSP 1 Score: 161.384 bits (407), Expect = 3.660e-39 Identity = 125/352 (35.51%), Postives = 184/352 (52.27%), Query Frame = 1
Query: 121 LINQWNSSRLELFKISQPDKNLKFFGVMRFFFE--TEDKFHSKCLRVSSDLKVKDLLPILLEKFYVSDICSSKQDFRLFERSSSIDKLLDPEDFPLLIQLFWCKDDKEGVFVFK-----------SNLKNKNSRRIRSQTRKDKIGSTTCLNSPTTRC-LDKVCTD----PPTKSTK---QXXXXXXXXXXXXXXXRTISNPXXXXXXXXXXXXXXXXXQISKS-GNLNGGGTLKIYGDSLNPLIPYKSLLLSIDDTAEFVVREILDKYGMENENENDFSLVSSVY--KDQNTQKIEYEEILQNSDSPLRFLLQSNKNDGFIKFRVVKMPANI---------SKSKKSKTQG 1077
+IN WN++RL+LF+ISQP ++L+F GVMRF+F+ F +KC+RVSS +D++ L EKF S + L+E S ++ LD E+ PL++QL W KDD+EG FV K SN K + Q K + + K TD P + Q + + +RTISNPE +M+ +R+ KLE+++++ S G + GGTL+IY DSL P IPYK++LLS +DTA+F V E L+KYGME EN ++ + V Q++ K E IL + + PL G + F++ + P + SKS KSK +G
Sbjct: 15 IINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPRYSLYEVHVSGERKLDFEEKPLVVQLNWNKDDREGRFVLKNENDIFPAKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKRRERDILKQSTDNEDRPQVRDDSENLQLAMEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTNDTADFAVIEALEKYGMEKENPKEYCIAKVVLPPGAQHSDKGMKEFILDDEECPLHIFRDWPSEKGELVFQLKRRPTDSLLKQNRKHESKSGKSKERG 366 HSP 2 Score: 154.066 bits (388), Expect = 6.308e-37 Identity = 120/394 (30.46%), Postives = 193/394 (48.98%), Query Frame = 1
Query: 1567 LPVQITYFKSKEDMLLDSILLDIKPSQTKFKLASAYLLHLMVVDIIR----------------LTCINRATVLDQELVIGMFIEKVASKIRQSIMQHRTIISIQSYWLANCTEFLHLLKNNILLSQFNSSIFDKYYDLFADNAQWCFQCISFGFTDDMVNTLSVLLNENTDDSEEKDDFPLSRKASDPKNMSILIRTLSSCMSVLQQCHVNPALIIQIISRIFHLINCWIFNSFIDTP----CRIFRGWAXXXXXXXXXXXXWSERHDLLLAADCYFQQSLQACKLLIMEKTDIEVFYNETVDMVDINSIQLRYLLDNYIYSPNEPKVPQIWIDFVVSGANQVADKVFEEEGIDICXXXXXXXXXXXXXXXDGYHVDSLYGGVPVGFIEYLEKL 2688
LP I + ++ ED L +I+ S FKL+ Y+L++ ++ + +N+ ++GM +E V +I + + + I ++W+AN +E L+ +K + N D D+ A Q F+ + ++ +++ + DD EE D + PK +L TL+ MS+L++C VN AL IQ+ S++FH IN W+FN + P C + W II ++ I W+E+ L LAADC+ + +QA LL M+K + N +NS+QL+ LL NY +P+EP +P ID VVS A AD++ +G ++ L+ L LP LLPEDGY D + +P G E+LE L
Sbjct: 517 LPANIEFRENSEDAFLSAIINYTNSSTVHFKLSPTYVLYMTCRYVLSNKYRPDTSPAERTHKVIAVVNK--------MVGM-MESVIQEIDKGNQKQKNIAGALAFWMANASELLNFIKQD---RDLNRITLDAQ-DVLAHLVQMAFKYLVHCLQSELTSSMPAFI----DDPEEND-------SQRPKIEDVL-HTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHY--WGAIIRQQLAHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSHQDIPNINNTCFKLNSLQLQALLQNYHCAPDEPYIPTELIDSVVSVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVR-NIPNGLQEFLEPL 882
BLAST of Afadin vs. Ensembl Xenopus
Match: AFDN (afadin, adherens junction formation factor [Source:NCBI gene;Acc:100493083]) HSP 1 Score: 159.458 bits (402), Expect = 1.650e-38 Identity = 123/355 (34.65%), Postives = 183/355 (51.55%), Query Frame = 1
Query: 121 LINQWNSSRLELFKISQPDKNLKFFGVMRFFFE--TEDKFHSKCLRVSSDLKVKDLLPILLEKFYVSDICSSKQDFRLFE-RSSSIDKLLDPEDFPLLIQLFWCKDDKEGVFVFK------------SNLKNKNSRRIRSQTRKDKIGSTTC---------LNSPTTRCLDKVCTDPPTKSTKQXXXXXXXXXXXXXXXRTISNPXXXXXXXXXXXXXXXXXQISKS-GNLNGGGTLKIYGDSLNPLIPYKSLLLSIDDTAEFVVREILDKYGMENENENDFSLVSSVY--KDQNTQKIEYEEILQNSDSPLRFLLQSNKNDGFIKFRVVKMPANI---------SKSKKSKTQG 1077
+IN WN++RL+LF+ISQP ++L+F GVMRF+F+ F +KC+RVSS +D++ L EKF S + L+E S ++ LD E+ PL++QL W KDD+EG FV K SN K + Q K + + +T D+ Q + + +RTISNPE +M+ +R+ KLE+++++ S G + GGTL+IY DSL P IPYK++LLS +DTA+F V E L+KYGME EN ++ + V Q++ K E IL + + PL G + F++ + P + SKS KSK +G
Sbjct: 15 IINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPRYSLYEVHVSGEERKLDFEEKPLVVQLNWNKDDREGRFVLKNENDIFPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKRRERDILKQSTDNEDRALAHGGDYENLQLAMEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTNDTADFAVIEALEKYGMEKENPKEYCIAKVVLPPGAQHSDKGMKEFILDDEECPLHIFRDWPSEKGELVFQLKRRPTDSLLKQNRKHESKSGKSKERG 369 HSP 2 Score: 148.673 bits (374), Expect = 2.776e-35 Identity = 117/387 (30.23%), Postives = 191/387 (49.35%), Query Frame = 1
Query: 1567 LPVQITYFKSKEDMLLDSILLDIKPSQTKFKLASAYLLHLMVVDIIRLTCINRATVLDQELVIGMFIEKVASKIRQSIMQ---------HRTIISIQSYWLANCTEFLHLLKNNILLSQFNSSIFDKYYDLFADNAQWCFQCISFGFTDDMVNTLSVLLNENTDDSEEKDDFPLSRKASDPKNMSILIRTLSSCMSVLQQCHVNPALIIQIISRIFHLINCWIFNSFIDTP----CRIFRGWAXXXXXXXXXXXXWSERHDLLLAADCYFQQSLQACKLLIMEKTDIEVFYNETVDMVDINSIQLRYLLDNYIYSPNEPKVPQIWIDFVVSGANQVADKVFEEEGIDICXXXXXXXXXXXXXXXDGYHVDSLYGGVPVGFIEYLEKL 2688
LP I + ++ ED L +I+ S FKL+ Y+L++ ++ + ++ + + K+ + +S++Q + I ++W+AN +E L+ +K + N D D+ A Q F+ + ++ +++ + DD EE D + PK +L TL+ MS+L++C VN AL IQ+ S++FH IN W+FN + P C + W II ++ I W+E+ L LAADC+ + +QA LL M+K + N +NS+QL+ LL NY +P+EP +P ID VVS A AD++ +G ++ L+ L LP LLPEDGY D + +P G E+LE L
Sbjct: 520 LPANIEFRENSEDAFLSAIINYTNSSTVHFKLSPTYVLYMTCRYVLSNKYRPDTSPAERTHKVIAVVNKMVGMM-ESVIQVSNQVGHEKQKNIAGALAFWMANASELLNFIKQD---RDLNRITLDAQ-DVLAHLVQMAFKYLVHCLQSELTSSMPAFI----DDPEEND-------SQRPKIEDVL-HTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHY--WGAIIRQQLAHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSHQDIPNINNTCFKLNSLQLQALLQNYHCAPDEPYIPTELIDSVVSVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVR-NIPNGLQEFLEPL 886
BLAST of Afadin vs. Ensembl Xenopus
Match: AFDN (afadin, adherens junction formation factor [Source:NCBI gene;Acc:100493083]) HSP 1 Score: 159.073 bits (401), Expect = 2.180e-38 Identity = 124/354 (35.03%), Postives = 187/354 (52.82%), Query Frame = 1
Query: 121 LINQWNSSRLELFKISQPDKNLKFFGVMRFFFE--TEDKFHSKCLRVSSDLKVKDLLPILLEKFYVSDICSSKQDFRLFE-RSSSIDKLLDPEDFPLLIQLFWCKDDKEGVFVFKSN-----LKNKNSRRIRSQTRKDKIGS-TTCLNSPTTRCLDKVCTDPPTKSTK--------------QXXXXXXXXXXXXXXXRTISNPXXXXXXXXXXXXXXXXXQISKS-GNLNGGGTLKIYGDSLNPLIPYKSLLLSIDDTAEFVVREILDKYGMENENENDFSLVSSVY--KDQNTQKIEYEEILQNSDSPLRFLLQSNKNDGFIKFRVVKMPANI---------SKSKKSKTQG 1077
+IN WN++RL+LF+ISQP ++L+F GVMRF+F+ F +KC+RVSS +D++ L EKF S + L+E S ++ LD E+ PL++QL W KDD+EG FV K+ K S Q ++ I + L+ + + D +ST Q + + +RTISNPE +M+ +R+ KLE+++++ S G + GGTL+IY DSL P IPYK++LLS +DTA+F V E L+KYGME EN ++ + V Q++ K E IL + + PL G + F++ + P + SKS KSK +G
Sbjct: 15 IINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPRYSLYEVHVSGEERKLDFEEKPLVVQLNWNKDDREGRFVLKNENDIFPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKRRERDILKQSTDNEDRPQVRDDSENLQLAMEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTNDTADFAVIEALEKYGMEKENPKEYCIAKVVLPPGAQHSDKGMKEFILDDEECPLHIFRDWPSEKGELVFQLKRRPTDSLLKQNRKHESKSGKSKERG 368 HSP 2 Score: 157.147 bits (396), Expect = 7.587e-38 Identity = 124/412 (30.10%), Postives = 197/412 (47.82%), Query Frame = 1
Query: 1567 LPVQITYFKSKEDMLLDSILLDIKPSQTKFKLASAYLLHLMVVDIIRLTCINRATVLDQELVIGMFIEKVASKIRQSIMQHRTIISIQSYWLANCTEFLHLLKNNILLSQFNSSIFDKYYDLFADNAQWCFQCISFGFTDDMVNTLSVLLNENTDDSEEKDDFPLSRKASDPKNMSILIRTLSSCMSVLQQCHVNPALIIQIISRIFHLINCWIFNSFIDTP----CRIFRGWAXXXXXXXXXXXXWSERHDLLLAADCYFQQSLQACKLLIMEKTDIEVFYNETVDMVDINSIQLRYLLDNYIYSPNEPKVPQIWIDFVVSGANQVADKVFEEEGIDICXXXXXXXXXXXXXXXDGYHVDSLYGGVPVGFIEYLEKLHKEWCFYKISHQDIHFDWLCQKRGFQDTSCELLP 2790
LP I + ++ ED L +I+ S FKL+ Y+L++ ++ + ++ + + K+ + I + + I ++W+AN +E L+ +K + N D D+ A Q F+ + ++ +++ + DD EE D + PK +L TL+ MS+L++C VN AL IQ+ S++FH IN W+FN + P C + W II ++ I W+E+ L LAADC+ + +QA LL M+K + N +NS+QL+ LL NY +P+EP +P ID VVS A AD++ +G ++ L+ L LP LLPEDGY D + +P G E+LE LCQ RGF C L+P
Sbjct: 541 LPANIEFRENSEDAFLSAIINYTNSSTVHFKLSPTYVLYMTCRYVLSNKYRPDTSPAERTHKVIAVVNKMVGMMESVIQKQKNIAGALAFWMANASELLNFIKQD---RDLNRITLDAQ-DVLAHLVQMAFKYLVHCLQSELTSSMPAFI----DDPEEND-------SQRPKIEDVL-HTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHY--WGAIIRQQLAHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSHQDIPNINNTCFKLNSLQLQALLQNYHCAPDEPYIPTELIDSVVSVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVR-NIPNGLQEFLEP-------------------LCQ-RGF----CRLVP 909
BLAST of Afadin vs. Ensembl Mouse
Match: Afdn (afadin, adherens junction formation factor [Source:MGI Symbol;Acc:MGI:1314653]) HSP 1 Score: 156.377 bits (394), Expect = 1.023e-37 Identity = 120/366 (32.79%), Postives = 187/366 (51.09%), Query Frame = 1
Query: 121 LINQWNSSRLELFKISQPDKNLKFFGVMRFFFE--TEDKFHSKCLRVSSDLKVKDLLPILLEKFYVSDICSSKQDFRLFERSSSIDKLLDPEDFPLLIQLFWCKDDKEGVFVF------------KSNLKNKNSRR-------------------------IRSQTRKDKIGSTTCLNSPTTRCLDKVCTDPPTKSTKQXXXXXXXXXXXXXXXRTISNPXXXXXXXXXXXXXXXXXQISKS-GNLNGGGTLKIYGDSLNPLIPYKSLLLSIDDTAEFVVREILDKYGMENENENDFSLV------SSVYKDQNTQKIEYEEILQNSDSPLRFLLQSNKNDGFIKFRVVKMPAN-ISKSKKSKTQG 1077
+I+ WN++RL+LF+ISQP ++L+F GVMRF+F+ F +KC+RVSS +D++ L EKF S + L+E S ++ LD ++ PL++QL W KDD+EG FV +SN K + +R + K++ S +S +R +V D P S +RTISNPE +M+ +R+ KLE+++++ S G + GGTL+IY DSL P IPYK++LLS DTA+F V E L+KYG+E EN D+ + + + D+ K E IL + + PL+ + + G + F++ + P + I K K +G
Sbjct: 16 IIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKKEKEALRQASDKEERPSQGD-DSENSRLAAEVYKDMPETS----------------FTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDTADFAVAESLEKYGLEKENPKDYCIARVMLPPGAQHSDERGAK---EIILDDDECPLQIFREWPSDKGILVFQLKRRPPDYIPKKMKKHVEG 361 HSP 2 Score: 154.451 bits (389), Expect = 4.551e-37 Identity = 131/414 (31.64%), Postives = 201/414 (48.55%), Query Frame = 1
Query: 1567 LPVQITYFKSKEDMLLDSILLDIKPSQTKFKLASAYLLHLMVVDIIRLTCINRATVLDQELVIGMF--IEKVASKIRQSIMQHRTIISIQSYWLANCTEFLHLLKNNILLSQFNSSIFDKYYDLFADNAQWCFQCISFGFTDDMVNTLSVLLNENTDDSEEKDDFPLSRKASDPKNMSILIRTLSSCMSVLQQCHVNPALIIQIISRIFHLINCWIFNSFIDTP----CRIFRGWAXXXXXXXXXXXXWSERHDLLLAADCYFQQSLQACKLLIMEKTDIEVFYNETVDMVDINSIQLRYLLDNYIYSPNEPKVPQIWIDFVVSGANQVADKVFEEEGIDICXXXXXXXXXXXXXXXDGYHVDSLYGGVPVGFIEYLEKLHKEWCFYKISHQDIHFDWLCQKRGFQDTSCELLP 2790
LP I + +S ED L +I+ S FKL+ Y+L++ + L+ +R + E + K+ S + I + + I ++W+AN +E L+ +K + LS+ I D+ A Q F+ + ++ N + L DD EE L R PK + ++ TL+ MS+L++C VN AL IQ+ S++FH IN W+FN + P C + W II ++ I W+E+ L LAADC+ + +QA LL M+K + N +NS+QL+ LL NY +P+EP +P I+ VV+ A AD++ +G D+ L+ L LP LLPEDGY D + +P G E+L D LCQ RGF C L+P
Sbjct: 601 LPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYV--LSSQHRPDISPTERTHKAIAVVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSR----ITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFL----DDPEENS---LQR----PK-IDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHY--WGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYVPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVAVAENTADELARSDGRDVQLEEDPDLQLPFLLPEDGYSCDVVR-NIPNGLQEFL-------------------DPLCQ-RGF----CRLVP 969
BLAST of Afadin vs. Ensembl Mouse
Match: Afdn (afadin, adherens junction formation factor [Source:MGI Symbol;Acc:MGI:1314653]) HSP 1 Score: 156.377 bits (394), Expect = 1.088e-37 Identity = 120/366 (32.79%), Postives = 187/366 (51.09%), Query Frame = 1
Query: 121 LINQWNSSRLELFKISQPDKNLKFFGVMRFFFE--TEDKFHSKCLRVSSDLKVKDLLPILLEKFYVSDICSSKQDFRLFERSSSIDKLLDPEDFPLLIQLFWCKDDKEGVFVF------------KSNLKNKNSRR-------------------------IRSQTRKDKIGSTTCLNSPTTRCLDKVCTDPPTKSTKQXXXXXXXXXXXXXXXRTISNPXXXXXXXXXXXXXXXXXQISKS-GNLNGGGTLKIYGDSLNPLIPYKSLLLSIDDTAEFVVREILDKYGMENENENDFSLV------SSVYKDQNTQKIEYEEILQNSDSPLRFLLQSNKNDGFIKFRVVKMPAN-ISKSKKSKTQG 1077
+I+ WN++RL+LF+ISQP ++L+F GVMRF+F+ F +KC+RVSS +D++ L EKF S + L+E S ++ LD ++ PL++QL W KDD+EG FV +SN K + +R + K++ S +S +R +V D P S +RTISNPE +M+ +R+ KLE+++++ S G + GGTL+IY DSL P IPYK++LLS DTA+F V E L+KYG+E EN D+ + + + D+ K E IL + + PL+ + + G + F++ + P + I K K +G
Sbjct: 16 IIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKKEKEALRQASDKEERPSQGD-DSENSRLAAEVYKDMPETS----------------FTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDTADFAVAESLEKYGLEKENPKDYCIARVMLPPGAQHSDERGAK---EIILDDDECPLQIFREWPSDKGILVFQLKRRPPDYIPKKMKKHVEG 361 HSP 2 Score: 153.68 bits (387), Expect = 7.914e-37 Identity = 132/421 (31.35%), Postives = 203/421 (48.22%), Query Frame = 1
Query: 1567 LPVQITYFKSKEDMLLDSILLDIKPSQTKFKLASAYLLHLMV---------VDIIRLTCINRATVLDQELVIGMFIEKVASKIRQSIMQHRTIISIQSYWLANCTEFLHLLKNNILLSQFNSSIFDKYYDLFADNAQWCFQCISFGFTDDMVNTLSVLLNENTDDSEEKDDFPLSRKASDPKNMSILIRTLSSCMSVLQQCHVNPALIIQIISRIFHLINCWIFNSFIDTP----CRIFRGWAXXXXXXXXXXXXWSERHDLLLAADCYFQQSLQACKLLIMEKTDIEVFYNETVDMVDINSIQLRYLLDNYIYSPNEPKVPQIWIDFVVSGANQVADKVFEEEGIDICXXXXXXXXXXXXXXXDGYHVDSLYGGVPVGFIEYLEKLHKEWCFYKISHQDIHFDWLCQKRGFQDTSCELLP 2790
LP I + +S ED L +I+ S FKL+ Y+L++ DI ++A + ++V +E V ++ Q + + I ++W+AN +E L+ +K + LS+ I D+ A Q F+ + ++ N + L DD EE L R PK + ++ TL+ MS+L++C VN AL IQ+ S++FH IN W+FN + P C + W II ++ I W+E+ L LAADC+ + +QA LL M+K + N +NS+QL+ LL NY +P+EP +P I+ VV+ A AD++ +G D+ L+ L LP LLPEDGY D + +P G E+L D LCQ RGF C L+P
Sbjct: 601 LPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQHRPDISPTERTHKAIAVVNKMV--SMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSR----ITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFL----DDPEENS---LQR----PK-IDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHY--WGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYVPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVAVAENTADELARSDGRDVQLEEDPDLQLPFLLPEDGYSCDVVR-NIPNGLQEFL-------------------DPLCQ-RGF----CRLVP 976
BLAST of Afadin vs. Ensembl Mouse
Match: Afdn (afadin, adherens junction formation factor [Source:MGI Symbol;Acc:MGI:1314653]) HSP 1 Score: 156.377 bits (394), Expect = 1.131e-37 Identity = 120/366 (32.79%), Postives = 187/366 (51.09%), Query Frame = 1
Query: 121 LINQWNSSRLELFKISQPDKNLKFFGVMRFFFE--TEDKFHSKCLRVSSDLKVKDLLPILLEKFYVSDICSSKQDFRLFERSSSIDKLLDPEDFPLLIQLFWCKDDKEGVFVF------------KSNLKNKNSRR-------------------------IRSQTRKDKIGSTTCLNSPTTRCLDKVCTDPPTKSTKQXXXXXXXXXXXXXXXRTISNPXXXXXXXXXXXXXXXXXQISKS-GNLNGGGTLKIYGDSLNPLIPYKSLLLSIDDTAEFVVREILDKYGMENENENDFSLV------SSVYKDQNTQKIEYEEILQNSDSPLRFLLQSNKNDGFIKFRVVKMPAN-ISKSKKSKTQG 1077
+I+ WN++RL+LF+ISQP ++L+F GVMRF+F+ F +KC+RVSS +D++ L EKF S + L+E S ++ LD ++ PL++QL W KDD+EG FV +SN K + +R + K++ S +S +R +V D P S +RTISNPE +M+ +R+ KLE+++++ S G + GGTL+IY DSL P IPYK++LLS DTA+F V E L+KYG+E EN D+ + + + D+ K E IL + + PL+ + + G + F++ + P + I K K +G
Sbjct: 16 IIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKKEKEALRQASDKEERPSQGD-DSENSRLAAEVYKDMPETS----------------FTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDTADFAVAESLEKYGLEKENPKDYCIARVMLPPGAQHSDERGAK---EIILDDDECPLQIFREWPSDKGILVFQLKRRPPDYIPKKMKKHVEG 361 HSP 2 Score: 152.91 bits (385), Expect = 1.091e-36 Identity = 131/414 (31.64%), Postives = 201/414 (48.55%), Query Frame = 1
Query: 1567 LPVQITYFKSKEDMLLDSILLDIKPSQTKFKLASAYLLHLMVVDIIRLTCINRATVLDQELVIGMF--IEKVASKIRQSIMQHRTIISIQSYWLANCTEFLHLLKNNILLSQFNSSIFDKYYDLFADNAQWCFQCISFGFTDDMVNTLSVLLNENTDDSEEKDDFPLSRKASDPKNMSILIRTLSSCMSVLQQCHVNPALIIQIISRIFHLINCWIFNSFIDTP----CRIFRGWAXXXXXXXXXXXXWSERHDLLLAADCYFQQSLQACKLLIMEKTDIEVFYNETVDMVDINSIQLRYLLDNYIYSPNEPKVPQIWIDFVVSGANQVADKVFEEEGIDICXXXXXXXXXXXXXXXDGYHVDSLYGGVPVGFIEYLEKLHKEWCFYKISHQDIHFDWLCQKRGFQDTSCELLP 2790
LP I + +S ED L +I+ S FKL+ Y+L++ + L+ +R + E + K+ S + I + + I ++W+AN +E L+ +K + LS+ I D+ A Q F+ + ++ N + L DD EE L R PK + ++ TL+ MS+L++C VN AL IQ+ S++FH IN W+FN + P C + W II ++ I W+E+ L LAADC+ + +QA LL M+K + N +NS+QL+ LL NY +P+EP +P I+ VV+ A AD++ +G D+ L+ L LP LLPEDGY D + +P G E+L D LCQ RGF C L+P
Sbjct: 601 LPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYV--LSSQHRPDISPTERTHKAIAVVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSR----ITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFL----DDPEENS---LQR----PK-IDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHY--WGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYVPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVAVAENTADELARSDGRDVQLEEDPDLQLPFLLPEDGYSCD-VVRNIPNGLQEFL-------------------DPLCQ-RGF----CRLVP 969
BLAST of Afadin vs. Ensembl Mouse
Match: Afdn (afadin, adherens junction formation factor [Source:MGI Symbol;Acc:MGI:1314653]) HSP 1 Score: 156.377 bits (394), Expect = 1.216e-37 Identity = 120/366 (32.79%), Postives = 187/366 (51.09%), Query Frame = 1
Query: 121 LINQWNSSRLELFKISQPDKNLKFFGVMRFFFE--TEDKFHSKCLRVSSDLKVKDLLPILLEKFYVSDICSSKQDFRLFERSSSIDKLLDPEDFPLLIQLFWCKDDKEGVFVF------------KSNLKNKNSRR-------------------------IRSQTRKDKIGSTTCLNSPTTRCLDKVCTDPPTKSTKQXXXXXXXXXXXXXXXRTISNPXXXXXXXXXXXXXXXXXQISKS-GNLNGGGTLKIYGDSLNPLIPYKSLLLSIDDTAEFVVREILDKYGMENENENDFSLV------SSVYKDQNTQKIEYEEILQNSDSPLRFLLQSNKNDGFIKFRVVKMPAN-ISKSKKSKTQG 1077
+I+ WN++RL+LF+ISQP ++L+F GVMRF+F+ F +KC+RVSS +D++ L EKF S + L+E S ++ LD ++ PL++QL W KDD+EG FV +SN K + +R + K++ S +S +R +V D P S +RTISNPE +M+ +R+ KLE+++++ S G + GGTL+IY DSL P IPYK++LLS DTA+F V E L+KYG+E EN D+ + + + D+ K E IL + + PL+ + + G + F++ + P + I K K +G
Sbjct: 16 IIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKKEKEALRQASDKEERPSQGD-DSENSRLAAEVYKDMPETS----------------FTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDTADFAVAESLEKYGLEKENPKDYCIARVMLPPGAQHSDERGAK---EIILDDDECPLQIFREWPSDKGILVFQLKRRPPDYIPKKMKKHVEG 361 HSP 2 Score: 154.451 bits (389), Expect = 4.303e-37 Identity = 131/414 (31.64%), Postives = 201/414 (48.55%), Query Frame = 1
Query: 1567 LPVQITYFKSKEDMLLDSILLDIKPSQTKFKLASAYLLHLMVVDIIRLTCINRATVLDQELVIGMF--IEKVASKIRQSIMQHRTIISIQSYWLANCTEFLHLLKNNILLSQFNSSIFDKYYDLFADNAQWCFQCISFGFTDDMVNTLSVLLNENTDDSEEKDDFPLSRKASDPKNMSILIRTLSSCMSVLQQCHVNPALIIQIISRIFHLINCWIFNSFIDTP----CRIFRGWAXXXXXXXXXXXXWSERHDLLLAADCYFQQSLQACKLLIMEKTDIEVFYNETVDMVDINSIQLRYLLDNYIYSPNEPKVPQIWIDFVVSGANQVADKVFEEEGIDICXXXXXXXXXXXXXXXDGYHVDSLYGGVPVGFIEYLEKLHKEWCFYKISHQDIHFDWLCQKRGFQDTSCELLP 2790
LP I + +S ED L +I+ S FKL+ Y+L++ + L+ +R + E + K+ S + I + + I ++W+AN +E L+ +K + LS+ I D+ A Q F+ + ++ N + L DD EE L R PK + ++ TL+ MS+L++C VN AL IQ+ S++FH IN W+FN + P C + W II ++ I W+E+ L LAADC+ + +QA LL M+K + N +NS+QL+ LL NY +P+EP +P I+ VV+ A AD++ +G D+ L+ L LP LLPEDGY D + +P G E+L D LCQ RGF C L+P
Sbjct: 586 LPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYV--LSSQHRPDISPTERTHKAIAVVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSR----ITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFL----DDPEENS---LQR----PK-IDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHY--WGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYVPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVAVAENTADELARSDGRDVQLEEDPDLQLPFLLPEDGYSCD-VVRNIPNGLQEFL-------------------DPLCQ-RGF----CRLVP 954
BLAST of Afadin vs. Ensembl Mouse
Match: Afdn (afadin, adherens junction formation factor [Source:MGI Symbol;Acc:MGI:1314653]) HSP 1 Score: 156.377 bits (394), Expect = 1.248e-37 Identity = 120/366 (32.79%), Postives = 187/366 (51.09%), Query Frame = 1
Query: 121 LINQWNSSRLELFKISQPDKNLKFFGVMRFFFE--TEDKFHSKCLRVSSDLKVKDLLPILLEKFYVSDICSSKQDFRLFERSSSIDKLLDPEDFPLLIQLFWCKDDKEGVFVF------------KSNLKNKNSRR-------------------------IRSQTRKDKIGSTTCLNSPTTRCLDKVCTDPPTKSTKQXXXXXXXXXXXXXXXRTISNPXXXXXXXXXXXXXXXXXQISKS-GNLNGGGTLKIYGDSLNPLIPYKSLLLSIDDTAEFVVREILDKYGMENENENDFSLV------SSVYKDQNTQKIEYEEILQNSDSPLRFLLQSNKNDGFIKFRVVKMPAN-ISKSKKSKTQG 1077
+I+ WN++RL+LF+ISQP ++L+F GVMRF+F+ F +KC+RVSS +D++ L EKF S + L+E S ++ LD ++ PL++QL W KDD+EG FV +SN K + +R + K++ S +S +R +V D P S +RTISNPE +M+ +R+ KLE+++++ S G + GGTL+IY DSL P IPYK++LLS DTA+F V E L+KYG+E EN D+ + + + D+ K E IL + + PL+ + + G + F++ + P + I K K +G
Sbjct: 16 IIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKKEKEALRQASDKEERPSQGD-DSENSRLAAEVYKDMPETS----------------FTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDTADFAVAESLEKYGLEKENPKDYCIARVMLPPGAQHSDERGAK---EIILDDDECPLQIFREWPSDKGILVFQLKRRPPDYIPKKMKKHVEG 361 HSP 2 Score: 154.451 bits (389), Expect = 4.845e-37 Identity = 131/414 (31.64%), Postives = 201/414 (48.55%), Query Frame = 1
Query: 1567 LPVQITYFKSKEDMLLDSILLDIKPSQTKFKLASAYLLHLMVVDIIRLTCINRATVLDQELVIGMF--IEKVASKIRQSIMQHRTIISIQSYWLANCTEFLHLLKNNILLSQFNSSIFDKYYDLFADNAQWCFQCISFGFTDDMVNTLSVLLNENTDDSEEKDDFPLSRKASDPKNMSILIRTLSSCMSVLQQCHVNPALIIQIISRIFHLINCWIFNSFIDTP----CRIFRGWAXXXXXXXXXXXXWSERHDLLLAADCYFQQSLQACKLLIMEKTDIEVFYNETVDMVDINSIQLRYLLDNYIYSPNEPKVPQIWIDFVVSGANQVADKVFEEEGIDICXXXXXXXXXXXXXXXDGYHVDSLYGGVPVGFIEYLEKLHKEWCFYKISHQDIHFDWLCQKRGFQDTSCELLP 2790
LP I + +S ED L +I+ S FKL+ Y+L++ + L+ +R + E + K+ S + I + + I ++W+AN +E L+ +K + LS+ I D+ A Q F+ + ++ N + L DD EE L R PK + ++ TL+ MS+L++C VN AL IQ+ S++FH IN W+FN + P C + W II ++ I W+E+ L LAADC+ + +QA LL M+K + N +NS+QL+ LL NY +P+EP +P I+ VV+ A AD++ +G D+ L+ L LP LLPEDGY D + +P G E+L D LCQ RGF C L+P
Sbjct: 586 LPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYV--LSSQHRPDISPTERTHKAIAVVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSR----ITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFL----DDPEENS---LQR----PK-IDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHY--WGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYVPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVAVAENTADELARSDGRDVQLEEDPDLQLPFLLPEDGYSCD-VVRNIPNGLQEFL-------------------DPLCQ-RGF----CRLVP 954
BLAST of Afadin vs. UniProt/SwissProt
Match: sp|Q9QZQ1|AFAD_MOUSE (Afadin OS=Mus musculus OX=10090 GN=Afdn PE=1 SV=3) HSP 1 Score: 156.377 bits (394), Expect = 7.156e-37 Identity = 120/366 (32.79%), Postives = 187/366 (51.09%), Query Frame = 1
Query: 121 LINQWNSSRLELFKISQPDKNLKFFGVMRFFFE--TEDKFHSKCLRVSSDLKVKDLLPILLEKFYVSDICSSKQDFRLFERSSSIDKLLDPEDFPLLIQLFWCKDDKEGVFVF------------KSNLKNKNSRR-------------------------IRSQTRKDKIGSTTCLNSPTTRCLDKVCTDPPTKSTKQXXXXXXXXXXXXXXXRTISNPXXXXXXXXXXXXXXXXXQISKS-GNLNGGGTLKIYGDSLNPLIPYKSLLLSIDDTAEFVVREILDKYGMENENENDFSLV------SSVYKDQNTQKIEYEEILQNSDSPLRFLLQSNKNDGFIKFRVVKMPAN-ISKSKKSKTQG 1077
+I+ WN++RL+LF+ISQP ++L+F GVMRF+F+ F +KC+RVSS +D++ L EKF S + L+E S ++ LD ++ PL++QL W KDD+EG FV +SN K + +R + K++ S +S +R +V D P S +RTISNPE +M+ +R+ KLE+++++ S G + GGTL+IY DSL P IPYK++LLS DTA+F V E L+KYG+E EN D+ + + + D+ K E IL + + PL+ + + G + F++ + P + I K K +G
Sbjct: 16 IIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKKEKEALRQASDKEERPSQGD-DSENSRLAAEVYKDMPETS----------------FTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDTADFAVAESLEKYGLEKENPKDYCIARVMLPPGAQHSDERGAK---EIILDDDECPLQIFREWPSDKGILVFQLKRRPPDYIPKKMKKHVEG 361 HSP 2 Score: 154.451 bits (389), Expect = 3.183e-36 Identity = 131/414 (31.64%), Postives = 201/414 (48.55%), Query Frame = 1
Query: 1567 LPVQITYFKSKEDMLLDSILLDIKPSQTKFKLASAYLLHLMVVDIIRLTCINRATVLDQELVIGMF--IEKVASKIRQSIMQHRTIISIQSYWLANCTEFLHLLKNNILLSQFNSSIFDKYYDLFADNAQWCFQCISFGFTDDMVNTLSVLLNENTDDSEEKDDFPLSRKASDPKNMSILIRTLSSCMSVLQQCHVNPALIIQIISRIFHLINCWIFNSFIDTP----CRIFRGWAXXXXXXXXXXXXWSERHDLLLAADCYFQQSLQACKLLIMEKTDIEVFYNETVDMVDINSIQLRYLLDNYIYSPNEPKVPQIWIDFVVSGANQVADKVFEEEGIDICXXXXXXXXXXXXXXXDGYHVDSLYGGVPVGFIEYLEKLHKEWCFYKISHQDIHFDWLCQKRGFQDTSCELLP 2790
LP I + +S ED L +I+ S FKL+ Y+L++ + L+ +R + E + K+ S + I + + I ++W+AN +E L+ +K + LS+ I D+ A Q F+ + ++ N + L DD EE L R PK + ++ TL+ MS+L++C VN AL IQ+ S++FH IN W+FN + P C + W II ++ I W+E+ L LAADC+ + +QA LL M+K + N +NS+QL+ LL NY +P+EP +P I+ VV+ A AD++ +G D+ L+ L LP LLPEDGY D + +P G E+L D LCQ RGF C L+P
Sbjct: 601 LPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYV--LSSQHRPDISPTERTHKAIAVVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSR----ITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFL----DDPEENS---LQR----PK-IDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHY--WGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYVPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVAVAENTADELARSDGRDVQLEEDPDLQLPFLLPEDGYSCDVVR-NIPNGLQEFL-------------------DPLCQ-RGF----CRLVP 969
BLAST of Afadin vs. UniProt/SwissProt
Match: sp|O35889|AFAD_RAT (Afadin OS=Rattus norvegicus OX=10116 GN=Afdn PE=1 SV=1) HSP 1 Score: 154.066 bits (388), Expect = 4.223e-36 Identity = 119/366 (32.51%), Postives = 186/366 (50.82%), Query Frame = 1
Query: 121 LINQWNSSRLELFKISQPDKNLKFFGVMRFFFE--TEDKFHSKCLRVSSDLKVKDLLPILLEKFYVSDICSSKQDFRLFERSSSIDKLLDPEDFPLLIQLFWCKDDKEGVFVF------------KSNLKNKNSRR-------------------------IRSQTRKDKIGSTTCLNSPTTRCLDKVCTDPPTKSTKQXXXXXXXXXXXXXXXRTISNPXXXXXXXXXXXXXXXXXQISKS-GNLNGGGTLKIYGDSLNPLIPYKSLLLSIDDTAEFVVREILDKYGMENENENDFSLV------SSVYKDQNTQKIEYEEILQNSDSPLRFLLQSNKNDGFIKFRVVKMPAN-ISKSKKSKTQG 1077
+I+ WN++RL+LF+ISQP ++L+F GVMRF+F+ F +KC+RVSS +D++ L EKF S + L+E S ++ LD ++ PL++QL W KDD+EG FV +SN K + +R + K++ S +S +R +V D P S +RTISNPE +M+ +R+ KLE+++++ S G + GGTL+IY DSL P IPYK++LLS D A+F V E L+KYG+E EN D+ + + + D+ K E IL + + PL+ + + G + F++ + P + I K K +G
Sbjct: 16 IIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPAKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKEERPSQGD-DSENSRLAAEVYKDMPETS----------------FTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAESLEKYGLEKENPKDYCIARVMLPPGAQHSDERGAK---EIILDDDECPLQIFREWPSDKGILVFQLKRRPPDYIPKKMKKHVEG 361 HSP 2 Score: 153.68 bits (387), Expect = 5.046e-36 Identity = 132/421 (31.35%), Postives = 203/421 (48.22%), Query Frame = 1
Query: 1567 LPVQITYFKSKEDMLLDSILLDIKPSQTKFKLASAYLLHLMV---------VDIIRLTCINRATVLDQELVIGMFIEKVASKIRQSIMQHRTIISIQSYWLANCTEFLHLLKNNILLSQFNSSIFDKYYDLFADNAQWCFQCISFGFTDDMVNTLSVLLNENTDDSEEKDDFPLSRKASDPKNMSILIRTLSSCMSVLQQCHVNPALIIQIISRIFHLINCWIFNSFIDTP----CRIFRGWAXXXXXXXXXXXXWSERHDLLLAADCYFQQSLQACKLLIMEKTDIEVFYNETVDMVDINSIQLRYLLDNYIYSPNEPKVPQIWIDFVVSGANQVADKVFEEEGIDICXXXXXXXXXXXXXXXDGYHVDSLYGGVPVGFIEYLEKLHKEWCFYKISHQDIHFDWLCQKRGFQDTSCELLP 2790
LP I + +S ED L +I+ S FKL+ Y+L++ DI ++A + ++V +E V ++ Q + + I ++W+AN +E L+ +K + LS+ I D+ A Q F+ + ++ N + L DD EE L R PK + ++ TL+ MS+L++C VN AL IQ+ S++FH IN W+FN + P C + W II ++ I W+E+ L LAADC+ + +QA LL M+K + N +NS+QL+ LL NY +P+EP +P I+ VV+ A AD++ +G D+ L+ L LP LLPEDGY D + +P G E+L D LCQ RGF C L+P
Sbjct: 601 LPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQHRPDISPTERTHKAIAVVNKMV--SMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSR----ITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFL----DDPEENS---LQR----PK-IDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHY--WGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYVPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVAVAENTADELARSDGRDVQLEEDPDLQLPFLLPEDGYSCD-VVRNIPNGLQEFL-------------------DPLCQ-RGF----CRLVP 976
BLAST of Afadin vs. UniProt/SwissProt
Match: sp|P55196|AFAD_HUMAN (Afadin OS=Homo sapiens OX=9606 GN=AFDN PE=1 SV=3) HSP 1 Score: 153.68 bits (387), Expect = 5.038e-36 Identity = 127/412 (30.83%), Postives = 200/412 (48.54%), Query Frame = 1
Query: 1567 LPVQITYFKSKEDMLLDSILLDIKPSQTKFKLASAYLLHLMVVDIIRLTCINRATVLDQELVIGMFIEKVASKIRQSIMQHRTIISIQSYWLANCTEFLHLLKNNILLSQFNSSIFDKYYDLFADNAQWCFQCISFGFTDDMVNTLSVLLNENTDDSEEKDDFPLSRKASDPKNMSILIRTLSSCMSVLQQCHVNPALIIQIISRIFHLINCWIFNSFIDTP----CRIFRGWAXXXXXXXXXXXXWSERHDLLLAADCYFQQSLQACKLLIMEKTDIEVFYNETVDMVDINSIQLRYLLDNYIYSPNEPKVPQIWIDFVVSGANQVADKVFEEEGIDICXXXXXXXXXXXXXXXDGYHVDSLYGGVPVGFIEYLEKLHKEWCFYKISHQDIHFDWLCQKRGFQDTSCELLP 2790
LP I + +S ED L +I+ S FKL+ Y+L++ ++ + ++ + + K+ S + I + + I ++W+AN +E L+ +K + LS+ I D+ A Q F+ + ++ N + L DD EE L R PK + ++ TL+ MS+L++C VN AL IQ+ S++FH IN W+FN + P C + W II ++ I W+E+ L LAADC+ + +QA LL M+K + N +NS+QL+ LL NY +P+EP +P I+ VV+ A AD++ +G ++ L+ L LP LLPEDGY D + +P G E+L D LCQ RGF C L+P
Sbjct: 601 LPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSR----ITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFL----DDPEENS---LQR----PK-IDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHY--WGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCD-VVRNIPNGLQEFL-------------------DPLCQ-RGF----CRLIP 969 HSP 2 Score: 142.124 bits (357), Expect = 1.662e-32 Identity = 119/368 (32.34%), Postives = 181/368 (49.18%), Query Frame = 1
Query: 121 LINQWNSSRLELFKISQPDKNLKFFGVMRFFFE--TEDKFHSKCLRVSSDLKVKDLLPILLEKFYVSDICSSKQDFRLFERSSSIDKLLDPEDFPLLIQLFWC----------------------------KDDKEGVFV-FKSNLKNKNSRR--------IRSQTRKD--KIGSTTCLNSPTTRCLDKVCTDPPTKSTKQXXXXXXXXXXXXXXXRTISNPXXXXXXXXXXXXXXXXXQISKS-GNLNGGGTLKIYGDSLNPLIPYKSLLLSIDDTAEFVVREILDKYGMENENENDFSLVSSVY---KDQNTQKIEYEEILQNSDSPLRFLLQSNKNDGFIKFRVVKMPAN-ISKSKKSKTQGTFP 1086
+I+ WN++RL+LF+ISQP ++L+F GVMRF+F+ F +KC+RVSS +D++ L EKF S + L+E S ++ LD ++ PL++QL W K +KEGV FK L K + +R + KD NS R +V D P S +RTISNPE +M+ +R+ KLE+++++ S G + GGTL+IY DSL P IPYK++LLS D A+F V E L+KYG+E EN D+ + + + +K E IL + + PL+ + + G + F++ + P + I K K +G P
Sbjct: 16 IIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENS---RLAAEVYKDMPETS----------------FTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTP 364
BLAST of Afadin vs. TrEMBL
Match: A0A0M8ZQR9 (Afadin OS=Melipona quadrifasciata OX=166423 GN=WN51_08083 PE=4 SV=1) HSP 1 Score: 278.485 bits (711), Expect = 7.776e-74 Identity = 259/901 (28.75%), Postives = 425/901 (47.17%), Query Frame = 1
Query: 121 LINQWNSSRLELFKISQPDKNLKFFGVMRFFFE-TEDKFHSKCLRVSSDLKVKDLLPILLEKFYVSDICSSKQDFRLFE-RSSSIDKLLDPEDFPLLIQLFWCKDDKEGVFVF-----KSNLKNKNSRRIRSQTRKDKIGSTTCLNSPTTRCLDKVCTDPPTKSTKQXXXX-------XXXXXXXXXXXRTISNPXXXXXXXXXXXXXXXXXQI-SKSGNLNGGGTLKIYGDSLNPLIPYKSLLLSIDDTAEFVVREILDKYGMENENENDFSLVSSVYKDQNTQKIEYEEILQNSDSPLRFLLQSNKNDGFIKFRVVKMPAN-ISKSKKSKTQGTFPDVCKSIAIRKSNSVNYRNSDVQSHSRNTCDGKFKHSLHPDFSNNYNGSNNKIYSTPPSSDSPVPLLKRVESEKKPLPTYEESVSALRSWRVKISNFPENHKPISYRGSTESFSLSSDYQEDSSYNTDSYESRNIPSVETPEQENDDVVIISDLDSHLSRHLPVQITYFKSKEDMLLDSILLDIKPSQTKFKLASAYLLHLMVVDIIRLTCINRATVLDQELV--IGMFIEKVASKIRQSIMQHRTIISIQSYWLANCTEFLHLLKNNILLSQFNSSIFDKYYDLFADNAQWCFQCISFGFTDDMVNTLSVLLNENTDDSEEKDDFPLSRKASDPKNMSILIRTLSSCMSVLQQCHVNPALIIQIISRIFHLINCWIFNSFIDTPCRIFRGWAXXXXXXXXXXXXWSERHDLLLAADCYFQQSLQACKLLIMEKTDIEVFYNETVDMVDINSIQLRYLLDNYIYSPNEPKVPQIWIDFVVSGANQVADKVFEEEGIDICXXXXXXXXXXXXXXXDGYHVDSLYGGVPVGFIEYLEKLHKE--------------WCFYKISHQD 2727
+I QWN++RL+LF++S+P+++L+F GVMRF+F+ + K +KC+RV+SD + ++ L+EKF S ++ L+E + ++ L E+ PLL+QL W DD+EG F+ K+N + + + K+ L ++ KS +Q + + + +R+ISNPE++MR +R+ KLE KL+Q SK G + GGTLKIYG++L +PYK+LLLS+ D+A VVRE+L KYG+E + + LV ++ + E IL + + PL L+ G I F V + P++ + + +K K G K N V+YR D + F L+PD + NG+ + P +++ +R S P+ SV A V + P H P S +F L + E +S + +R + + P + D + LP + + + E+ +++ D++PS +FKLA Y L+L R + R + E + + + VAS I++ I + S + WLAN +E LH+LKN+ + F++ D + +CIS M ++ A +P + +++ SS M++L++C VN AL IQ+ S +FH IN FN+ + R + R + R+ + W+ER L LA+ C+ +QA LL K + E + +NS+Q+R LL Y + +EP++P I+ VV A VAD + +G +I L+ + +L L LLLPEDGY + + GVP G E+L L ++ W Y I H +
Sbjct: 18 VIQQWNANRLDLFELSEPNEDLEFHGVMRFYFQDSGQKVATKCIRVASDATSQAVIETLIEKFRPDMRMLSVPEYALYEIHENGEERKLGLEEKPLLVQLNWHIDDREGRFLLRRIDDKTNAQGVGFSSSDGSSFRRKLSKREKKQMKKQEKLSRL------KSLEQDENAIPVDQNGVAEKLYTASFTRSISNPEAVMRRRRQQKLERKLQQFRSKDGGPDTGGTLKIYGEALCKDVPYKTLLLSVRDSAVQVVREMLTKYGLEKVDPQQYCLVQVNSENNMNGGTQQEYILDDDECPLAILMNHPSARGSIMFHVRRRPSDYVPRKRKKKPSG------------KWNEVDYRYEDERL--------PFSLELNPDGNEIANGAGVRHRLQPNAAEMGS---ERSISIHPSSPSKSTSVPAAAVATVCHTRSP-THPPESTHNYETTFDLDGNV-ETASLTSSRDGNRTLQNDRQP-RGMDPI-------------LPAVLEFLEETEETFFHAVITDVEPSAPQFKLAPTYTLYLAAR--YRASTHYRPELQPTERAHRLTVMLANVASMIQRVIQERYMDTSSLALWLANGSELLHMLKNDRHVGAFSTRAQDILTEAVHTAFASLVRCISLELAPAMSQFMA--------------------DADEPAKEAGILQIFSSTMALLRRCRVNAALTIQLFSHLFHTINATAFNALVSNANLCVRWFGRRLKGRLNALETWAERQGLELASQCHLATIMQATHLLQAPKYNAEELATLSSTCFKLNSLQVRALLQKYQPAADEPRLPAELIENVVKVAESVADTLARADGREIRLEEEPTLALALLLPEDGYSCEVIR-GVPPGLAEFLTPLQRDGLCRMAPQPTSSGYWTIYMIDHNN 850
BLAST of Afadin vs. TrEMBL
Match: A0A1I8MFH0 (Uncharacterized protein OS=Musca domestica OX=7370 GN=101892464 PE=4 SV=1) HSP 1 Score: 274.633 bits (701), Expect = 8.231e-71 Identity = 256/891 (28.73%), Postives = 437/891 (49.05%), Query Frame = 1
Query: 121 LINQWNSSRLELFKISQPDKNLKFFGVMRFFFE-TEDKFHSKCLRVSSDLKVKDLLPILLEKFYVSDICSSKQDFRLFE-RSSSIDKLLDPEDFPLLIQLFWCKDDKEGVFVFKS-------------NLKNKNSRRIRSQTRKDKI-------GSTTCLNSPTTRCLDKVCTDPPTKSTKQXXXXXXXXXXXXXXXRTISNPXXXXXXXXXXXXXXXXXQI-SKSGNLNGGGTLKIYGDSLNPLIPYKSLLLSIDDTAEFVVREILDKYGMENENENDFSLVSSVYKDQNTQKIEYEEILQNSDSPLRFLLQSNKNDGFIKFRVVKMPANIS-KSKKSKTQGTFPDVCKSIAIRKSNSVNY-RNSDVQSHSRNTCDGKFKHSLHPDFSNNYNGSNNKIYSTPPSSDSPVPLLKRVESEKKPLPTYEESVSALRSWRVKISNFPENHKPISYRGSTESFSLSSDYQEDSSYNTDSYESRNIPSVETPEQENDDVVIISDLDSHLSRH-----LPVQITYFKSKEDMLLDSILLDIKPSQTKFKLASAYLLHLMVVDIIRLTCINRATVLDQELV--IGMFIEKVASKIRQSIMQHRTIISIQSYWLANCTEFLHLLKNNILLSQFNSSIFDKYYDLFADNAQWCFQCISFGFTDDMVNTLSVLLNENTDDSEEKDDFPLSRKASDPKNMSILIRTLSSCMSVLQQCHVNPALIIQIISRIFHLINCWIFNSFIDTPCRIFRGWAXXXXXXXXXXXXWSERHDLLLAADCYFQQSLQACKLLIMEKTDIEVFYNETVDMVDINSIQLRYLLDNYIYSPNEPKVPQIWIDFVVSGANQVADKVFEEEGIDICXXXXXXXXXXXXXXXDGYHVDSLYGGVPVGFIEYLEKLHKE 2697
+I QWN++RL+LF +S+PD+NL F GVMRF+F+ K +KC+RV+SD V D++ L+EKF S ++ L+E ++ ++ L+P++ PLL+QL W DD+EG F+ K+ N K K S+R + + +K + GS T NS T TD Q + + +R+ISNPE++MR +R+ KLE+KL+Q S+ G + GGTLKIYG+SL +PYK+LLLSI D A+ VVRE+L KYG++ + + LV N+ EY IL + + PL L+ + G I F V + PA+ + +K K G A +N+++ R + ++ DG + L D + + N + PS + L+ VE P + ++++ + + N + + G S+ D D YN +S+ + S E+ + + +H+ LP + + ++ +++ L I+ ++ + FKLA Y L+L R + R + E + +F+ VA+ I + + T I ++W+AN +EFLH LK++ +S F+ + ++ A++ Q F+ + F ++ TL+ L+E D +++ L S M++L++C VN AL IQ+ S++FH IN FN+ + W +++ R++ + W+ER L LAADC+ + Q + L KT +E +NS+Q+ LL + K+P+ +D + A VAD++ +G ++ L+ L+L LLLP+DG+ D + G+P G +++L L ++
Sbjct: 18 VIQQWNANRLDLFALSEPDENLLFHGVMRFYFQDAGQKVATKCIRVASDATVIDVIDTLIEKFRPDMRMLSVPNYALYEVHANGEERRLNPDEKPLLVQLNWHIDDREGRFLLKNIDQKTTPIEQNDINFKRKLSKREKKEQKKKEKLAKLNTDGSNTTTNSQTNGLNGSHGTD---AGETQVAGKLYTELPETSFTRSISNPEAVMRRRRQQKLEKKLQQFRSRDGGPDTGGTLKIYGESLCQDVPYKTLLLSIRDCAQAVVREMLTKYGLDKADPLHYCLVQV-----NSDGSEY--ILDDDECPLSILMNHPTSRGSIMFHVRRRPADSQPRRRKKKPLG---------AANGANNISQDREGPMLVEVTHSGDGGRRIKLGSDPIEVGSANTNCLQLFGPSIQARHCLISLVEGVCTVTPLHADALTFVNGHHISQPTILHNGSVVMF-GRVASYRFV-DSPTDGRYNLALSQSQ-LDSACLYERSPKMPLSSTSAAAHIEPQGQEPILPAVLEFPETHQELFLRHIITELDVNAPHFKLAPVYSLYLCAR--YRASTHYRPELQPTERAHKLTIFLHHVANLIYNVVQEQYTDPRILAFWMANSSEFLHFLKSDRHISAFSV----QAQEVLAESVQTAFRNLVNCFRVELSQTLNQFLSETIDHDSAA---------------GLVLTVLGSAMALLRRCRVNAALTIQLFSQLFHYINVICFNTIVANSHMCTGEWGKVMTERLQLLELWAERQGLELAADCHLAKINQCAQFLQAPKTSVEEIQQLACSCFRLNSLQMAALLQ-------QEKIPRNLVDTAIRMAESVADELTRADGREVRLEESPELHLALLLPDDGFSCDVVR-GIPSGLVDFLNPLQQQ 857
BLAST of Afadin vs. TrEMBL
Match: A0A4S2KFE7 (Afadin (Fragment) OS=Temnothorax longispinosus OX=300112 GN=DBV15_10157 PE=4 SV=1) HSP 1 Score: 273.478 bits (698), Expect = 2.943e-70 Identity = 263/936 (28.10%), Postives = 432/936 (46.15%), Query Frame = 1
Query: 121 LINQWNSSRLELFKISQPDKNLKFFGVMRFFFE-TEDKFHSKCLRVSSDLKVKDLLPILLEKFYVSDICSSKQDFRLFE-RSSSIDKLLDPEDFPLLIQLFWCKDDKEGVFVFKSNLKNKNSRRIRSQTRKDKIGSTTCLNSPTTRCLDKVCTDPPT--------KSTKQXXXXXXXXXXXXXX-----------XRTISNPXXXXXXXXXXXXXXXXXQI-SKSGNLNGGGTLKIYGDSLNPLIPYKSLLLSIDDTAEFVVREILDKYGMENENENDFSLVSSVYKDQNTQKIEYEEILQNSDSPLRFLLQSNKNDGFIKFRVVKMPANISKSKKSKTQGTFPDVCKSIAIRKSNSVNYRNSDVQSHSRNTCDGKFKHSLHPDFSNNYNGSNNKIYSTPP----SSDSPV-PLLKRVESEKKPLPT---------YEESVSAL----RSWRVKISNFPENHKPISYRGSTESFSLSSDYQEDSSYNTDSYESRNIPSVETPEQENDDVVIISDLDSHLSRH-------LPVQITYFKSKEDMLLDSILLDIKPSQTKFKLASAYLLHLMVVDIIRLTCINRATVLDQELV--IGMFIEKVASKIRQSIMQHRTIISIQSYWLANCTEFLHLLKNNILLSQFNSSIFDKYYDLFADNAQWCF----QCISFGFTDDMVNTLSVLLNENTDDSEEKDDFPLSRKASDPKNMSILIRTLSSCMSVLQQCHVNPALIIQIISRIFHLINCWIFNSFIDTPCRIFRGWAXXXXXXXXXXXXWSERHDLLLAADCYFQQSLQACKLLIMEKTDIEVFYNETVDMVDINSIQLRYLLDNYIYSPNEPKVPQIWIDFVVSGANQVADKVFEEEGIDICXXXXXXXXXXXXXXXDGYHVDSLYGGVPVGFIEYLEKLHKE--------------WCFYKISHQD 2727
+I QWN++RL+LF++S+P+++L+F GVMRF+F+ + K +KC+RV+SD + ++ L+EKF S ++ L+E + ++ LD E+ PLL+QL W DD+EG F+ K RI +T +G + S R L K KS +Q + +R+ISNPE++MR +R+ KLE +L+Q SK G + GGTLKIYG++L +PYK+LLLS+ D+A VVRE+L KYG++ + + LV V + E IL + + PL L+ G I F V + PA+ P K K N +++R D + F L+PD S+ NG+ + P S+ P+ P + ++ P PT + E++ L R ++N + I G+ F ++ + + E R + + N + D S R LP + + + E+ L +++ D++PS +FKLA Y L+L R + + + E + + + VA+ I++ I + S ++WLAN +E LH+LK++ + F++ + D+ AD F +C++ T M ++ A +P + +++ S+ M++L++C VN AL IQ+ S +FH IN FN+ + R + R + R+ + W+ER L LA+ C+ +QA LL K + E + +NS+Q+R LL Y + +EP++P I+ VV A VAD + +G +I L+ + L L LLLPEDGY + + GVP G E+L L ++ W Y I H +
Sbjct: 153 VIQQWNANRLDLFELSEPNEDLEFHGVMRFYFQDSGQKVATKCIRVASDATSQAVIETLIEKFRPDMRMLSVPEYALYEIHENGEERKLDLEEKPLLVQLNWHIDDREGRFLLK---------RIDDKTNAQGVGFISAEGSSFRRKLSKREKKQIKKQEKLSRLKSLEQDENAAPGDPNGVAEKLYTELPETSFTRSISNPEAVMRRRRQQKLERRLQQFRSKDGGPDTGGTLKIYGEALCKDVPYKTLLLSVRDSAAQVVREMLSKYGLDKVDPQLYCLVQ-VNSENVGGNAHQEYILDDDECPLAILMNHPSTRGSIMFHVRRRPADY-----------MPRKRKKKPAGKWNELDHRYEDERL--------PFLLELNPDGSDIPNGAGVRHRLQPNVTEVGSERPIGPQAVQAQTLTLPGPTVMPRHCVIAFTENIVTLTPCSRDAHTYVNNQRIHQTTILQNGAIIKFGRMHTFR----FIDPAPEER----IRQRQDSNKQIDYAYDRHSQYDRQPRGTDPILPAVLEFLEETEETFLHAVITDVEPSAPQFKLAPTYTLYLSAR--YRASTHYKPELQPTERAHRLTVMLANVATMIQRVIQERYMDASSLAFWLANGSELLHMLKSDRHVGAFST----RAQDILADAVHTAFGSLVRCVTLELTPAMTQFMA--------------------DADEPAKEAGVLQIFSNTMALLRRCRVNAALTIQLFSHLFHAINATAFNTLVSNGNLCVRWFGRRLKARLNALETWAERQGLELASQCHLATIMQATHLLQAPKYNAEELATLSSTCFKLNSLQVRALLQKYQPAADEPRLPAELIENVVRVAESVADTLARADGREIRLEEEPVLGLALLLPEDGYSCEVIR-GVPPGLAEFLAPLQRDGLCRMAAQPTSSGYWTIYMIDHHN 1024
BLAST of Afadin vs. TrEMBL
Match: A0A1J1HL25 (CLUMA_CG000755, isoform E OS=Clunio marinus OX=568069 GN=putative Afadin PE=4 SV=1) HSP 1 Score: 272.322 bits (695), Expect = 3.443e-70 Identity = 254/898 (28.29%), Postives = 424/898 (47.22%), Query Frame = 1
Query: 121 LINQWNSSRLELFKISQPDKNLKFFGVMRFFFE-TEDKFHSKCLRVSSDLKVKDLLPILLEKFYVSDICSSKQDFRLFE-RSSSIDKLLDPEDFPLLIQLFWCKDDKEGVFVFK--------------SNLKNKNSRRIRSQTRKDKIGSTTCLNSPTT--RCLDKVCTDPPTKSTKQXXXXXXXXXXXXXXXRTISNPXXXXXXXXXXXXXXXXXQI-SKSGNLNGGGTLKIYGDSLNPLIPYKSLLLSIDDTAEFVVREILDKYGMENENENDFSLVSSVYKDQNTQKIEYEEILQNSDSPLRFLLQSNKNDGFIKFRVVKMPANISKSKKSKTQGTFPDVCKSIAIRKSNSVNYRNSDVQSHSRNTCDGKFKHSLHPDFSNNYNGSNNKIYSTPPSSDSPVPLLKRVESEKKPLPTYEESVSALRSWRVKISNFPENHKPISYRGSTESFSLSSDYQ-----EDSSYN-------TDS---YESR---------NIPSVETPEQENDDVVIISDLDSHLSRHLPVQITYFKSKEDMLLDSILLDIKPSQTKFKLASAYLLHLMVVDIIRLTCINRATVLDQELV--IGMFIEKVASKIRQSIMQHRTIISIQSYWLANCTEFLHLLKNNILLSQFNSSIFDKYYDLFADNAQWCFQCISFGFTDDMVNTLSVLLNENTDDSEEKDDFPLSRKASDPKNMSILIRTLSSCMSVLQQCHVNPALIIQIISRIFHLINCWIFNSFIDTPCRIFRGWAXXXXXXXXXXXXWSERHDLLLAADCYFQQSLQACKLLIMEKTDIEVFYNETVDMVDINSIQLRYLLDNYIYSPNEPKVPQIWIDFVVSGANQVADKVFEEEGIDICXXXXXXXXXXXXXXXDGYHVDSLYGGVPVGFIEYL 2679
+I QWN++RL+LF++S+P++NL+F GVMRF+F+ K +KC+RV+SD V D++ L+EKF S ++ L+E ++ ++ L+P++ PLL+QL W DD+EG F+ K +N K K S+R + + +K + N + +K+ T+ P S +R+ISNPE++MR +R+ KLE+KL+Q ++ G + GGTLKIYG+SL +PYK+LLLSI D A+ VVRE+L KYGM+ + + LV N+ EY IL + + PL L+ + G I F V + PA + + + + I+ N R V ++ DG + L + + + N + PS L+ E P + + ++ + + I + GS F ++ Y+ D YN DS YE+R + + T E + + + LP + + + ++ L ++ ++ + FKLA Y L+L R + R + E + +F+ VA+ I I + I S+W+AN +EFLH LK++ +S F+ + ++ A+ Q FQ + F ++ TL+ L+EN D +++ L S M++L++C VN AL IQ+ S++FH IN FN F+ T W + + +R+ + W+E+ L LAADC+ + Q + L KT++ +NS+Q+ LL ++ +P+ ID V A VAD++ +G +I L+ L L LLLP DG+ D + G+P G +++L
Sbjct: 19 VIQQWNANRLDLFELSEPNENLEFHGVMRFYFQDVGQKVATKCIRVASDATVADVIETLIEKFRPDMRMLSVPNYALYEVHANGEERKLNPDEKPLLVQLNWHVDDREGRFLLKDLDQKPTTLDNNTDTNFKRKLSKREKKEQKKKEKLQKLNPNGEDSENHVAEKLYTELPETS----------------FTRSISNPEAVMRRRRQQKLEKKLQQFRARDGGPDTGGTLKIYGESLCRDVPYKTLLLSIRDCAQAVVREMLAKYGMDKADPLHYCLVQV-----NSDGSEY--ILDDDECPLSILMNHPTSRGSIMFHVRRRPAADTSQPRRRKKKPL-----GISNGSGNMSAEREGPVLIEITHSGDGGRRLKLTTEPVEVGSANTNALQLFGPSIQPRHCLISLHEGVCTVTPLHADGLTFVNGHHI-------TQPTILHNGSVVMFGRAASYRFVDVPSDGRYNLALSQSQLDSAYLYENRPQSFAKPPAGMSATPTMEPQGQEAI------------LPAVLEFPEHHQEQFLKQVISELDVNSPNFKLAPVYTLYLCAR--YRASTHYRPDLQPTERAHKLTVFLHHVANLIHNVIQEKYNDPKILSFWMANSSEFLHFLKSDRHISAFSV----QAQEVLAECVQTAFQNLVSIFHSELSQTLNQFLSENIDHDSAA---------------GLVLSVLGSAMALLRRCRVNAALTIQLFSQLFHYINVVCFNKFVTTSHMCTSTWGKALSDRLSLLELWAEKQGLELAADCHLAKINQCAEFLQAPKTNVTEVQQLACSCFRLNSLQMAALL-------SQETIPRTLIDATVRMAESVADELTRADGREIRLEESPDLPLALLLPVDGFSCD-VVRGIPAGLVDFL 840
BLAST of Afadin vs. TrEMBL
Match: A0A1J1HG24 (CLUMA_CG000755, isoform C OS=Clunio marinus OX=568069 GN=putative Afadin PE=4 SV=1) HSP 1 Score: 272.707 bits (696), Expect = 3.658e-70 Identity = 254/898 (28.29%), Postives = 424/898 (47.22%), Query Frame = 1
Query: 121 LINQWNSSRLELFKISQPDKNLKFFGVMRFFFE-TEDKFHSKCLRVSSDLKVKDLLPILLEKFYVSDICSSKQDFRLFE-RSSSIDKLLDPEDFPLLIQLFWCKDDKEGVFVFK--------------SNLKNKNSRRIRSQTRKDKIGSTTCLNSPTT--RCLDKVCTDPPTKSTKQXXXXXXXXXXXXXXXRTISNPXXXXXXXXXXXXXXXXXQI-SKSGNLNGGGTLKIYGDSLNPLIPYKSLLLSIDDTAEFVVREILDKYGMENENENDFSLVSSVYKDQNTQKIEYEEILQNSDSPLRFLLQSNKNDGFIKFRVVKMPANISKSKKSKTQGTFPDVCKSIAIRKSNSVNYRNSDVQSHSRNTCDGKFKHSLHPDFSNNYNGSNNKIYSTPPSSDSPVPLLKRVESEKKPLPTYEESVSALRSWRVKISNFPENHKPISYRGSTESFSLSSDYQ-----EDSSYN-------TDS---YESR---------NIPSVETPEQENDDVVIISDLDSHLSRHLPVQITYFKSKEDMLLDSILLDIKPSQTKFKLASAYLLHLMVVDIIRLTCINRATVLDQELV--IGMFIEKVASKIRQSIMQHRTIISIQSYWLANCTEFLHLLKNNILLSQFNSSIFDKYYDLFADNAQWCFQCISFGFTDDMVNTLSVLLNENTDDSEEKDDFPLSRKASDPKNMSILIRTLSSCMSVLQQCHVNPALIIQIISRIFHLINCWIFNSFIDTPCRIFRGWAXXXXXXXXXXXXWSERHDLLLAADCYFQQSLQACKLLIMEKTDIEVFYNETVDMVDINSIQLRYLLDNYIYSPNEPKVPQIWIDFVVSGANQVADKVFEEEGIDICXXXXXXXXXXXXXXXDGYHVDSLYGGVPVGFIEYL 2679
+I QWN++RL+LF++S+P++NL+F GVMRF+F+ K +KC+RV+SD V D++ L+EKF S ++ L+E ++ ++ L+P++ PLL+QL W DD+EG F+ K +N K K S+R + + +K + N + +K+ T+ P S +R+ISNPE++MR +R+ KLE+KL+Q ++ G + GGTLKIYG+SL +PYK+LLLSI D A+ VVRE+L KYGM+ + + LV N+ EY IL + + PL L+ + G I F V + PA + + + + I+ N R V ++ DG + L + + + N + PS L+ E P + + ++ + + I + GS F ++ Y+ D YN DS YE+R + + T E + + + LP + + + ++ L ++ ++ + FKLA Y L+L R + R + E + +F+ VA+ I I + I S+W+AN +EFLH LK++ +S F+ + ++ A+ Q FQ + F ++ TL+ L+EN D +++ L S M++L++C VN AL IQ+ S++FH IN FN F+ T W + + +R+ + W+E+ L LAADC+ + Q + L KT++ +NS+Q+ LL ++ +P+ ID V A VAD++ +G +I L+ L L LLLP DG+ D + G+P G +++L
Sbjct: 19 VIQQWNANRLDLFELSEPNENLEFHGVMRFYFQDVGQKVATKCIRVASDATVADVIETLIEKFRPDMRMLSVPNYALYEVHANGEERKLNPDEKPLLVQLNWHVDDREGRFLLKDLDQKPTTLDNNTDTNFKRKLSKREKKEQKKKEKLQKLNPNGEDSENHVAEKLYTELPETS----------------FTRSISNPEAVMRRRRQQKLEKKLQQFRARDGGPDTGGTLKIYGESLCRDVPYKTLLLSIRDCAQAVVREMLAKYGMDKADPLHYCLVQV-----NSDGSEY--ILDDDECPLSILMNHPTSRGSIMFHVRRRPAADTSQPRRRKKKPL-----GISNGSGNMSAEREGPVLIEITHSGDGGRRLKLTTEPVEVGSANTNALQLFGPSIQPRHCLISLHEGVCTVTPLHADGLTFVNGHHI-------TQPTILHNGSVVMFGRAASYRFVDVPSDGRYNLALSQSQLDSAYLYENRPQSFAKPPAGMSATPTMEPQGQEAI------------LPAVLEFPEHHQEQFLKQVISELDVNSPNFKLAPVYTLYLCAR--YRASTHYRPDLQPTERAHKLTVFLHHVANLIHNVIQEKYNDPKILSFWMANSSEFLHFLKSDRHISAFSV----QAQEVLAECVQTAFQNLVSIFHSELSQTLNQFLSENIDHDSAA---------------GLVLSVLGSAMALLRRCRVNAALTIQLFSQLFHYINVVCFNKFVTTSHMCTSTWGKALSDRLSLLELWAEKQGLELAADCHLAKINQCAEFLQAPKTNVTEVQQLACSCFRLNSLQMAALL-------SQETIPRTLIDATVRMAESVADELTRADGREIRLEESPDLPLALLLPVDGFSCD-VVRGIPAGLVDFL 840
BLAST of Afadin vs. Ensembl Cavefish
Match: afdna (afadin, adherens junction formation factor a [Source:ZFIN;Acc:ZDB-GENE-050419-52]) HSP 1 Score: 160.999 bits (406), Expect = 3.684e-39 Identity = 117/378 (30.95%), Postives = 191/378 (50.53%), Query Frame = 1
Query: 1567 LPVQITYFKSKEDMLLDSILLDIKPSQTKFKLASAYLLHLMVVDIIRLTCINRATVLDQELVIGMFIEKVASKIRQSIMQHRTIISIQSYWLANCTEFLHLLKNNILLSQFNSSIFDKYYDLFADNAQWCFQCISFGFTDDMVNTLSVLLNENTDDSEEKDDFPLSRKASDPKNMSILIRTLSSCMSVLQQCHVNPALIIQIISRIFHLINCWIFNSFIDTP----CRIFRGWAXXXXXXXXXXXXWSERHDLLLAADCYFQQSLQACKLLIMEKTDIEVFYNETVDMVDINSIQLRYLLDNYIYSPNEPKVPQIWIDFVVSGANQVADKVFEEEGIDICXXXXXXXXXXXXXXXDGYHVDSLYGGVPVGFIEYLEKL 2688
LP I + ++ ED L +I+ S FKL+ Y+L++ ++ + ++ + + K+ S + I + + I ++W+AN +E L+ +K + LS+ I D+ A Q F+ + D+ N + L DD EE++ P K D ++ TL+ MS+L++C VN AL IQ+ S++FH IN W+FN + C + W I+ ++ I W+E+ L LAADC+ + +QA LL M+K ++ N +NS+QL L+ NY +P+EP +P ID VV+ A AD++ +G ++ L+ L LP LLPEDGY D + +P GF E+LE L
Sbjct: 573 LPASIEFRENSEDAFLSAIINYTNSSTVHFKLSPTYVLYMTCRYVMSSQYRPDISPSERTHKVIAIVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDKDLSR----ITLDAQDVLAHLVQMAFKYLVHCLQSDLNNYMPAFL----DDPEEQN--PQRPKIED------VLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNKLVTESDSGLCSHY--WGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSVQDVQNINNTCFKLNSLQLNALMSNYHCAPDEPYIPHELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVR-NIPNGFQEFLEPL 931 HSP 2 Score: 150.599 bits (379), Expect = 5.920e-36 Identity = 116/354 (32.77%), Postives = 181/354 (51.13%), Query Frame = 1
Query: 121 LINQWNSSRLELFKISQPDKNLKFFGVMRFFFE--TEDKFHSKCLRVSSDLKVKDLLPILLEKFYVSDICSSKQDFRLFE-RSSSIDKLLDPEDFPLLIQLFWCKDDKEG--VFVFKSNLKNKNSRRIRSQTRKDKI-----------------------------GSTTCLNSPT--TRCLDKVCTDPPTKSTKQXXXXXXXXXXXXXXXRTISNPXXXXXXXXXXXXXXXXXQI-SKSGNLNGGGTLKIYGDSLNPLIPYKSLLLSIDDTAEFVVREILDKYGMENENENDFSLVSSVYKDQNTQKIEYEEILQNSDSPLRFLLQSNKNDGFIKFRVVKMPANISKSKKSKT 1071
+IN WN++RL+LF+ISQP + L+F GVMRF+F+ F +KC+RVSS +D++ L EKF S + L+E S ++ LD ++ PL++QL W KDD+EG V ++++ K ++ + K+ + G LN +R +V D P S +RTISNPE +M+ +R+ KLE+++++ S G + GGTL+IY DSL P IPYK++LLS DTA+F V E L+KYG+E EN ++ + V+ D K E IL +++ PL+ + + F++ K P + K K+
Sbjct: 15 IINHWNANRLDLFEISQPTEELEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERKLDLDEKPLVVQLNWNKDDREGRFVLKNENDIIPKKAQSNGPEKEKEGVIQNFKRTLSKKEKKKEKKKDKEAFARIPDGDDHALNREDGDSRLAAEVYKDMPETS----------------FTRTISNPEVVMKRRRQQKLEKRMQEFRSGDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDTADFAVAEALEKYGLEKENPREYCIARVVHSDDKPVK---ETILDDTECPLQIFRDWTSDKEALVFQLKKRPPDYIAKKGRKS 349
BLAST of Afadin vs. Ensembl Cavefish
Match: afdna (afadin, adherens junction formation factor a [Source:ZFIN;Acc:ZDB-GENE-050419-52]) HSP 1 Score: 160.999 bits (406), Expect = 3.684e-39 Identity = 117/378 (30.95%), Postives = 191/378 (50.53%), Query Frame = 1
Query: 1567 LPVQITYFKSKEDMLLDSILLDIKPSQTKFKLASAYLLHLMVVDIIRLTCINRATVLDQELVIGMFIEKVASKIRQSIMQHRTIISIQSYWLANCTEFLHLLKNNILLSQFNSSIFDKYYDLFADNAQWCFQCISFGFTDDMVNTLSVLLNENTDDSEEKDDFPLSRKASDPKNMSILIRTLSSCMSVLQQCHVNPALIIQIISRIFHLINCWIFNSFIDTP----CRIFRGWAXXXXXXXXXXXXWSERHDLLLAADCYFQQSLQACKLLIMEKTDIEVFYNETVDMVDINSIQLRYLLDNYIYSPNEPKVPQIWIDFVVSGANQVADKVFEEEGIDICXXXXXXXXXXXXXXXDGYHVDSLYGGVPVGFIEYLEKL 2688
LP I + ++ ED L +I+ S FKL+ Y+L++ ++ + ++ + + K+ S + I + + I ++W+AN +E L+ +K + LS+ I D+ A Q F+ + D+ N + L DD EE++ P K D ++ TL+ MS+L++C VN AL IQ+ S++FH IN W+FN + C + W I+ ++ I W+E+ L LAADC+ + +QA LL M+K ++ N +NS+QL L+ NY +P+EP +P ID VV+ A AD++ +G ++ L+ L LP LLPEDGY D + +P GF E+LE L
Sbjct: 573 LPASIEFRENSEDAFLSAIINYTNSSTVHFKLSPTYVLYMTCRYVMSSQYRPDISPSERTHKVIAIVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDKDLSR----ITLDAQDVLAHLVQMAFKYLVHCLQSDLNNYMPAFL----DDPEEQN--PQRPKIED------VLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNKLVTESDSGLCSHY--WGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSVQDVQNINNTCFKLNSLQLNALMSNYHCAPDEPYIPHELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVR-NIPNGFQEFLEPL 931 HSP 2 Score: 150.599 bits (379), Expect = 5.920e-36 Identity = 116/354 (32.77%), Postives = 181/354 (51.13%), Query Frame = 1
Query: 121 LINQWNSSRLELFKISQPDKNLKFFGVMRFFFE--TEDKFHSKCLRVSSDLKVKDLLPILLEKFYVSDICSSKQDFRLFE-RSSSIDKLLDPEDFPLLIQLFWCKDDKEG--VFVFKSNLKNKNSRRIRSQTRKDKI-----------------------------GSTTCLNSPT--TRCLDKVCTDPPTKSTKQXXXXXXXXXXXXXXXRTISNPXXXXXXXXXXXXXXXXXQI-SKSGNLNGGGTLKIYGDSLNPLIPYKSLLLSIDDTAEFVVREILDKYGMENENENDFSLVSSVYKDQNTQKIEYEEILQNSDSPLRFLLQSNKNDGFIKFRVVKMPANISKSKKSKT 1071
+IN WN++RL+LF+ISQP + L+F GVMRF+F+ F +KC+RVSS +D++ L EKF S + L+E S ++ LD ++ PL++QL W KDD+EG V ++++ K ++ + K+ + G LN +R +V D P S +RTISNPE +M+ +R+ KLE+++++ S G + GGTL+IY DSL P IPYK++LLS DTA+F V E L+KYG+E EN ++ + V+ D K E IL +++ PL+ + + F++ K P + K K+
Sbjct: 15 IINHWNANRLDLFEISQPTEELEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERKLDLDEKPLVVQLNWNKDDREGRFVLKNENDIIPKKAQSNGPEKEKEGVIQNFKRTLSKKEKKKEKKKDKEAFARIPDGDDHALNREDGDSRLAAEVYKDMPETS----------------FTRTISNPEVVMKRRRQQKLEKRMQEFRSGDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDTADFAVAEALEKYGLEKENPREYCIARVVHSDDKPVK---ETILDDTECPLQIFRDWTSDKEALVFQLKKRPPDYIAKKGRKS 349
BLAST of Afadin vs. Ensembl Cavefish
Match: afdna (afadin, adherens junction formation factor a [Source:ZFIN;Acc:ZDB-GENE-050419-52]) HSP 1 Score: 160.614 bits (405), Expect = 4.094e-39 Identity = 117/378 (30.95%), Postives = 191/378 (50.53%), Query Frame = 1
Query: 1567 LPVQITYFKSKEDMLLDSILLDIKPSQTKFKLASAYLLHLMVVDIIRLTCINRATVLDQELVIGMFIEKVASKIRQSIMQHRTIISIQSYWLANCTEFLHLLKNNILLSQFNSSIFDKYYDLFADNAQWCFQCISFGFTDDMVNTLSVLLNENTDDSEEKDDFPLSRKASDPKNMSILIRTLSSCMSVLQQCHVNPALIIQIISRIFHLINCWIFNSFIDTP----CRIFRGWAXXXXXXXXXXXXWSERHDLLLAADCYFQQSLQACKLLIMEKTDIEVFYNETVDMVDINSIQLRYLLDNYIYSPNEPKVPQIWIDFVVSGANQVADKVFEEEGIDICXXXXXXXXXXXXXXXDGYHVDSLYGGVPVGFIEYLEKL 2688
LP I + ++ ED L +I+ S FKL+ Y+L++ ++ + ++ + + K+ S + I + + I ++W+AN +E L+ +K + LS+ I D+ A Q F+ + D+ N + L DD EE++ P K D ++ TL+ MS+L++C VN AL IQ+ S++FH IN W+FN + C + W I+ ++ I W+E+ L LAADC+ + +QA LL M+K ++ N +NS+QL L+ NY +P+EP +P ID VV+ A AD++ +G ++ L+ L LP LLPEDGY D + +P GF E+LE L
Sbjct: 558 LPASIEFRENSEDAFLSAIINYTNSSTVHFKLSPTYVLYMTCRYVMSSQYRPDISPSERTHKVIAIVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDKDLSR----ITLDAQDVLAHLVQMAFKYLVHCLQSDLNNYMPAFL----DDPEEQN--PQRPKIED------VLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNKLVTESDSGLCSHY--WGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSVQDVQNINNTCFKLNSLQLNALMSNYHCAPDEPYIPHELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVR-NIPNGFQEFLEPL 916 HSP 2 Score: 150.599 bits (379), Expect = 5.946e-36 Identity = 116/354 (32.77%), Postives = 181/354 (51.13%), Query Frame = 1
Query: 121 LINQWNSSRLELFKISQPDKNLKFFGVMRFFFE--TEDKFHSKCLRVSSDLKVKDLLPILLEKFYVSDICSSKQDFRLFE-RSSSIDKLLDPEDFPLLIQLFWCKDDKEG--VFVFKSNLKNKNSRRIRSQTRKDKI-----------------------------GSTTCLNSPT--TRCLDKVCTDPPTKSTKQXXXXXXXXXXXXXXXRTISNPXXXXXXXXXXXXXXXXXQI-SKSGNLNGGGTLKIYGDSLNPLIPYKSLLLSIDDTAEFVVREILDKYGMENENENDFSLVSSVYKDQNTQKIEYEEILQNSDSPLRFLLQSNKNDGFIKFRVVKMPANISKSKKSKT 1071
+IN WN++RL+LF+ISQP + L+F GVMRF+F+ F +KC+RVSS +D++ L EKF S + L+E S ++ LD ++ PL++QL W KDD+EG V ++++ K ++ + K+ + G LN +R +V D P S +RTISNPE +M+ +R+ KLE+++++ S G + GGTL+IY DSL P IPYK++LLS DTA+F V E L+KYG+E EN ++ + V+ D K E IL +++ PL+ + + F++ K P + K K+
Sbjct: 15 IINHWNANRLDLFEISQPTEELEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERKLDLDEKPLVVQLNWNKDDREGRFVLKNENDIIPKKAQSNGPEKEKEGVIQNFKRTLSKKEKKKEKKKDKEAFARIPDGDDHALNREDGDSRLAAEVYKDMPETS----------------FTRTISNPEVVMKRRRQQKLEKRMQEFRSGDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDTADFAVAEALEKYGLEKENPREYCIARVVHSDDKPVK---ETILDDTECPLQIFRDWTSDKEALVFQLKKRPPDYIAKKGRKS 349
BLAST of Afadin vs. Ensembl Cavefish
Match: afdna (afadin, adherens junction formation factor a [Source:ZFIN;Acc:ZDB-GENE-050419-52]) HSP 1 Score: 160.229 bits (404), Expect = 5.495e-39 Identity = 118/353 (33.43%), Postives = 182/353 (51.56%), Query Frame = 1
Query: 121 LINQWNSSRLELFKISQPDKNLKFFGVMRFFFE--TEDKFHSKCLRVSSDLKVKDLLPILLEKFYVSDICSSKQDFRLFERSSSIDKLLDPEDFPLLIQLFWCKDDKEGVFVFKS--NLKNKNSRRIRSQTRKDKI-----------------------------GSTTCLNSPT--TRCLDKVCTDPPTKSTKQXXXXXXXXXXXXXXXRTISNPXXXXXXXXXXXXXXXXXQI-SKSGNLNGGGTLKIYGDSLNPLIPYKSLLLSIDDTAEFVVREILDKYGMENENENDFSLVSSVYKDQNTQKIEYEEILQNSDSPLRFLLQSNKNDGFIKFRVVKMPANISKSKKSKT 1071
+IN WN++RL+LF+ISQP + L+F GVMRF+F+ F +KC+RVSS +D++ L EKF S + L+E S ++ LD ++ PL++QL W KDD+EG FV K+ ++ K ++ + K+ + G LN +R +V D P S +RTISNPE +M+ +R+ KLE+++++ S G + GGTL+IY DSL P IPYK++LLS DTA+F V E L+KYG+E EN ++ + V+ D K E IL +++ PL+ + + F++ K P + K K+
Sbjct: 15 IINHWNANRLDLFEISQPTEELEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERKLDLDEKPLVVQLNWNKDDREGRFVLKNENDIIPKKAQSNGPEKEKEGVIQNFKRTLSKKEKKKEKKKDKEAFARIPDGDDHALNREDGDSRLAAEVYKDMPETS----------------FTRTISNPEVVMKRRRQQKLEKRMQEFRSGDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDTADFAVAEALEKYGLEKENPREYCIARVVHSDDKPVK---ETILDDTECPLQIFRDWTSDKEALVFQLKKRPPDYIAKKGRKS 348 HSP 2 Score: 160.229 bits (404), Expect = 6.137e-39 Identity = 117/378 (30.95%), Postives = 191/378 (50.53%), Query Frame = 1
Query: 1567 LPVQITYFKSKEDMLLDSILLDIKPSQTKFKLASAYLLHLMVVDIIRLTCINRATVLDQELVIGMFIEKVASKIRQSIMQHRTIISIQSYWLANCTEFLHLLKNNILLSQFNSSIFDKYYDLFADNAQWCFQCISFGFTDDMVNTLSVLLNENTDDSEEKDDFPLSRKASDPKNMSILIRTLSSCMSVLQQCHVNPALIIQIISRIFHLINCWIFNSFIDTP----CRIFRGWAXXXXXXXXXXXXWSERHDLLLAADCYFQQSLQACKLLIMEKTDIEVFYNETVDMVDINSIQLRYLLDNYIYSPNEPKVPQIWIDFVVSGANQVADKVFEEEGIDICXXXXXXXXXXXXXXXDGYHVDSLYGGVPVGFIEYLEKL 2688
LP I + ++ ED L +I+ S FKL+ Y+L++ ++ + ++ + + K+ S + I + + I ++W+AN +E L+ +K + LS+ I D+ A Q F+ + D+ N + L DD EE++ P K D ++ TL+ MS+L++C VN AL IQ+ S++FH IN W+FN + C + W I+ ++ I W+E+ L LAADC+ + +QA LL M+K ++ N +NS+QL L+ NY +P+EP +P ID VV+ A AD++ +G ++ L+ L LP LLPEDGY D + +P GF E+LE L
Sbjct: 568 LPASIEFRENSEDAFLSAIINYTNSSTVHFKLSPTYVLYMTCRYVMSSQYRPDISPSERTHKVIAIVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDKDLSR----ITLDAQDVLAHLVQMAFKYLVHCLQSDLNNYMPAFL----DDPEEQN--PQRPKIED------VLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNKLVTESDSGLCSHY--WGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSVQDVQNINNTCFKLNSLQLNALMSNYHCAPDEPYIPHELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVR-NIPNGFQEFLEPL 926
BLAST of Afadin vs. Ensembl Cavefish
Match: afdna (afadin, adherens junction formation factor a [Source:ZFIN;Acc:ZDB-GENE-050419-52]) HSP 1 Score: 157.918 bits (398), Expect = 3.403e-38 Identity = 117/353 (33.14%), Postives = 182/353 (51.56%), Query Frame = 1
Query: 121 LINQWNSSRLELFKISQPDKNLKFFGVMRFFFE--TEDKFHSKCLRVSSDLKVKDLLPILLEKFYVSDICSSKQDFRLFERSSSIDKLLDPEDFPLLIQLFWCKDDKEGVFVF--KSNLKNKNSRRIRSQTRKDKI-----------------------------GSTTCLNSPT--TRCLDKVCTDPPTKSTKQXXXXXXXXXXXXXXXRTISNPXXXXXXXXXXXXXXXXXQI-SKSGNLNGGGTLKIYGDSLNPLIPYKSLLLSIDDTAEFVVREILDKYGMENENENDFSLVSSVYKDQNTQKIEYEEILQNSDSPLRFLLQSNKNDGFIKFRVVKMPANISKSKKSKT 1071
+IN WN++RL+LF+ISQP + L+F GVMRF+F+ F +KC+RVSS +D++ L EKF S + L+E S ++ LD ++ PL++QL W KDD+EG FV ++++ K ++ + K+ + G LN +R +V D P S +RTISNPE +M+ +R+ KLE+++++ S G + GGTL+IY DSL P IPYK++LLS DTA+F V E L+KYG+E EN ++ + V+ D K E IL +++ PL+ + + F++ K P + K K+
Sbjct: 15 IINHWNANRLDLFEISQPTEELEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERKLDLDEKPLVVQLNWNKDDREGRFVLKNENDIIPKKAQSNGPEKEKEGVIQNFKRTLSKKEKKKEKKKDKEAFARIPDGDDHALNREDGDSRLAAEVYKDMPETS----------------FTRTISNPEVVMKRRRQQKLEKRMQEFRSGDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDTADFAVAEALEKYGLEKENPREYCIARVVHSDDKPVK---ETILDDTECPLQIFRDWTSDKEALVFQLKKRPPDYIAKKGRKS 348 HSP 2 Score: 156.762 bits (395), Expect = 7.744e-38 Identity = 120/387 (31.01%), Postives = 192/387 (49.61%), Query Frame = 1
Query: 1567 LPVQITYFKSKEDMLLDSILLDIKPSQTKFKLASAYLLHLMV---------VDIIRLTCINRATVLDQELVIGMFIEKVASKIRQSIMQHRTIISIQSYWLANCTEFLHLLKNNILLSQFNSSIFDKYYDLFADNAQWCFQCISFGFTDDMVNTLSVLLNENTDDSEEKDDFPLSRKASDPKNMSILIRTLSSCMSVLQQCHVNPALIIQIISRIFHLINCWIFNSFIDTP----CRIFRGWAXXXXXXXXXXXXWSERHDLLLAADCYFQQSLQACKLLIMEKTDIEVFYNETVDMVDINSIQLRYLLDNYIYSPNEPKVPQIWIDFVVSGANQVADKVFEEEGIDICXXXXXXXXXXXXXXXDGYHVDSLYGGVPVGFIEYLEKL 2688
LP I + ++ ED L +I+ S FKL+ Y+L++ DI ++ + ++V +E V +I + + I ++W+AN +E L+ +K + LS+ I D+ A Q F+ + D+ N + L DD EE++ P K D ++ TL+ MS+L++C VN AL IQ+ S++FH IN W+FN + C + W I+ ++ I W+E+ L LAADC+ + +QA LL M+K ++ N +NS+QL L+ NY +P+EP +P ID VV+ A AD++ +G ++ L+ L LP LLPEDGY D + +P GF E+LE L
Sbjct: 572 LPASIEFRENSEDAFLSAIINYTNSSTVHFKLSPTYVLYMTCRYVMSSQYRPDISPSERTHKVIAIVNKMV--SMMEGVIQEIADGDQKQKNIAGALAFWMANASELLNFIKQDKDLSR----ITLDAQDVLAHLVQMAFKYLVHCLQSDLNNYMPAFL----DDPEEQN--PQRPKIED------VLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNKLVTESDSGLCSHY--WGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYSVQDVQNINNTCFKLNSLQLNALMSNYHCAPDEPYIPHELIDHVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVR-NIPNGFQEFLEPL 937
BLAST of Afadin vs. Ensembl Sea Lamprey
Match: ENSPMAT00000009066.1 (pep scaffold:Pmarinus_7.0:GL479913:2065:18580:1 gene:ENSPMAG00000008193.1 transcript:ENSPMAT00000009066.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 124.02 bits (310), Expect = 5.759e-29 Identity = 104/382 (27.23%), Postives = 174/382 (45.55%), Query Frame = 1
Query: 1567 LPVQITYFKSKEDMLLDSILL---DIKPSQTKFKLASAYLLH----LMVVDIIRLTCINRATVLDQELVIGMFIEKVASKIRQSIMQHRTIISIQSYWLANCTEFLHLLKNNILLSQFNSSIFDKYYDLFADNAQWCFQCISFGFTDDMVNTLSVLLNENTDDSEEKDDFPLSRKASDPKNMSILIRTLSSCMSVLQQCHVNPALIIQIISRIFHLINCWIFNSF---IDTPCRIFRGWAXXXXXXXXXXXXWSERHDLLLAADCYFQQSLQACKLLIMEKTDIEVFYNETVDMVDINSIQLRYLLDNYIYSPNEPKVPQIWIDFVVSGANQVADKVFEEEGIDICXXXXXXXXXXXXXXXDGYHVDSLYGGVPVGFIEYLE 2682
LPV + E+ LD++ DI PS F L AY ++ ++ + ++ + I E AS+I ++ + + +W+AN +E LH L+ + L ++ + F + ++ L V L + P + A P+ + ++ L+ MS+L+ NPAL++QI +R+F LI W+FNS +D R R WA + R+ + RW+E L AA C+ +QA +LL M K ++ V +NS+Q+R LL+ Y Y+P+EPK+PQ I+ VVS D V+ + ++ L LP L+P DGY + L +P G ++L+
Sbjct: 219 LPVYVEVSSIAEEEFLDAVTSTPSDIPPS---FSLGPAYTIYSACRFLLSSQGSCSLPSQQQQRRRHQHIAYLTEGAASRIEGTVQASGRDMMVSGFWMANTSELLHFLEQDGDLRPLTL----PAQEMLSSAVHTAFSNLVECLKTELSVRLPVFLQD-----------PQTLTAGAPQ-IGPVLSLLTEVMSMLRTFKTNPALLVQIFARVFTLIAGWLFNSLLSDVDLGLR-SRYWATTLQRRLLPLARWAEGQGLERAAACHLAHIVQATELLTMRKYEVSDVREILVTCYRLNSLQMRALLETYQYAPDEPKIPQEVIEQVVSVTRDTTDAVWLSQQQEVSRLESLELRLPFLVPGDGYACEGL-AAIPAGLHQFLQ 579
BLAST of Afadin vs. Ensembl Nematostella
Match: EDO32442 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SV26]) HSP 1 Score: 158.303 bits (399), Expect = 4.785e-39 Identity = 120/378 (31.75%), Postives = 195/378 (51.59%), Query Frame = 1
Query: 1567 LPVQITYFKSKEDMLLDSILLDIKPSQTKFKLASAYLLHLMVVDIIRLTCINRATVLDQELV--IGMFIEKVASKIRQSIMQHRTIISIQSYWLANCTEFLHLLKNNILLSQFNSSIFDKYYDLFADNAQWCFQCISFGFTDDMVNTLSVLLNENTDDSEEKDDFPLSRKASDPKNMSILIRTLSSCMSVLQQCHVNPALIIQIISRIFHLINCWIFNSFIDTPCRIF--RGWAXXXXXXXXXXXXWSERHDLLLAADCYFQQSLQACKLLIMEKTDIEVFYNETVDMVDINSIQLRYLLDNYIYSPNEPKVPQIWIDFVVSGANQVADKVFEEEGIDICXXXXXXXXXXXXXXXDGYHVDSLYGGVPVGFIEYLEKL 2688
LP + + +S ED +++ ++ FKLA Y L++ R++ R ++ Q+ +G+ I K+A+ R +I ++ + ++W+ANC+E LH +K +I +S + + A + Q F+ + ++ N+L L+++ D E D +R +S +I LSS MS+L++C VN AL IQ+ S++FH IN W+FN + P R W R I R+ R+ W+ R L LAADC+ + QA LL K+ + +NS+Q+R LL +Y + +E + Q +ID VV+ A AD+ + G +I L+ L LP LLPEDGY + + GVP G E+LE L
Sbjct: 576 LPATLEFRESGEDAFFAAVISEVNGLAVHFKLAPTYTLYM--CSRYRMSSAYRPELMPQQKAERLGVTIMKIANMARSTIRENSHMPGSLAFWMANCSELLHFIKEDIDISPYTKD----SQSVLAKSVQLAFRHLVLCIQHELRNSLPAFLDQSDDADVEDSDMEANRP-----TVSEVIYHLSSTMSLLRRCRVNAALTIQVFSQLFHFINMWLFNKLVLEPKLGLCSREWGRRISKRLRRVEDWALRQGLELAADCHLGRIEQAAYLLQAPKSSPADINAISSSCFKLNSVQMRTLLQSYRPTQDE-YLSQEFIDRVVAIAEDTADQTAYKSGQEITLEEDPELQLPFLLPEDGYSCEVIR-GVPQGLREFLEPL 940 HSP 2 Score: 137.502 bits (345), Expect = 1.243e-32 Identity = 115/355 (32.39%), Postives = 186/355 (52.39%), Query Frame = 1
Query: 112 ILHLINQWNSSRLELFKISQPDKNLKFFGVMRFFFE-TEDKFHSKCLRVSSDLKVKDLLPILLEKFYVSDICSSKQDFRLFERSSSIDKLLDPEDFPLLIQLFWCKDDKEGVFVFKSN----LKNKNSRRIRSQTRKDKIGSTTCLNSPTTRCLDKVCTDPPTKSTKQXXXXXXXXXXXXXXXRTISNPXXXXXXXXXXXXXXXXXQISKSGNLNGGGTLKIYGDSLNPLIPYKSLLLSIDDTAEFVVREILDKYGMENENENDFSLVSSVYKDQN---TQKIEYEEILQNSDSPLRFLLQSNKNDGFIKFRVVKMPANISKSK------KSKTQGTFPDVC--KSIAIRKSNSV 1128
+L LI +WN+SRL++F IS+P ++L++ GVMRFFF+ + K +KC+R+SS D+ IL++KF+ + + L+E + L D PL IQL W DD+EG F+ KS+ + KN + S + ++ K ++ + +R+ISNPE++MR +R+ KLE+KLK+ +S GGTLKI+ ++L P IPYK+LL+S+D+ +V+E L KY ME E+ ++ LV + + + E ++ + D PLR + +N G + F + + AN+ K + KS T+ DV KS+A R + S
Sbjct: 14 LLKLIEEWNASRLDIFAISEPTEDLEYRGVMRFFFQDSGSKVATKCVRISSKDNTDDVTKILVDKFHPDMKMLTNPSYSLYEVHPNGAHKLKATDHPLRIQLEWQLDDREGRFLLKSDQDKIVHAKNLFEGEQNQNLKRRLSKREKKQMKKKREEEAKKVKAGKDDTAIAQHLYDELPETSFTRSISNPEAVMRRRRQQKLEKKLKEF-ESRESAPGGTLKIFAETLKPEIPYKTLLVSVDEITTNIVKEALVKYNMEKEDPEEYCLVMVNIPPEGSNTSATLGKERVVHDEDCPLRIAVTWPENRGSVAFHLRRR-ANLPKYRRDKRRSKSPTRSPMQDVSPDKSVAERLAKST 366
BLAST of Afadin vs. Ensembl Medaka
Match: afdna (afadin, adherens junction formation factor [Source:NCBI gene;Acc:101169758]) HSP 1 Score: 159.073 bits (401), Expect = 1.328e-38 Identity = 121/358 (33.80%), Postives = 183/358 (51.12%), Query Frame = 1
Query: 121 LINQWNSSRLELFKISQPDKNLKFFGVMRFFFE--TEDKFHSKCLRVSSDLKVKDLLPILLEKFYVSDICSSKQDFRLFE-RSSSIDKLLDPEDFPLLIQLFWCKDDKEGVFVFK--SNLKNKNSRRIRSQTRKDKI----------------------------------GSTTCLNSPTTRCLDKVCTDPPTKSTKQXXXXXXXXXXXXXXXRTISNPXXXXXXXXXXXXXXXXXQ-ISKSGNLNGGGTLKIYGDSLNPLIPYKSLLLSIDDTAEFVVREILDKYGMENENENDFSLVSSVYKDQNTQKIEYEEILQNSDSPLRFLLQSNKNDGFIKFRVVKMPANISKSKKSKTQ 1074
+IN WN++RL+LF+ISQP ++L+F GVMRF+F+ F +KC+RVSS +D++ L EKF S + L+E S ++ LD ++ PL++QL W KDD+EG FV K +++ K S+ + KD + G NS R +V D P S +RTISNPE +M+ +R+ K+E++++ I+ G + GGTL+IY DSL P IPYK+LL S+ DTA+ VV E L+KYG+E EN +F + + +K E IL +S+ PL+ L + G + F++ K P + K K +
Sbjct: 15 IINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERQLDLDEKPLVVQLNWNKDDREGRFVLKNENDVPPKKSQSNGPEREKDGVIQNFKRTLSKKEKKKEKKREKEFIRMPDEDERTLGREDGENS---RLAAEVYKDMPETS----------------FTRTISNPEVVMKRRRQQKVEKRMQDFINNDGRPDSGGTLRIYADSLKPNIPYKTLLCSVRDTADLVVVEALEKYGLEKENPREFCIA------RQEEKTGKEVILDDSECPLQILRDWPADRGVLVFQLKKKPPDYQGRKGRKME 347 HSP 2 Score: 147.132 bits (370), Expect = 5.705e-35 Identity = 123/394 (31.22%), Postives = 192/394 (48.73%), Query Frame = 1
Query: 1567 LPVQITYFKSKEDMLLDSILLDIKPSQTKFKLASAYLLHLMVVDIIRLTCINRATVLDQEL---VIGM------FIEKVASKIRQSIMQHRTIISIQSYWLANCTEFLHLLKNNILLSQFNSSIFDKYYDLFADNAQWCFQCISFGFTDDMVNTLSVLLNENTDDSEEKDDFPLSRKASDPKNMSILIRTLSSCMSVLQQCHVNPALIIQIISRIFHLINCWIFNSFIDTP-----CRIFRGWAXXXXXXXXXXXXWSERHDLLLAADCYFQQSLQACKLLIMEKTDIEVFYNETVDMVDINSIQLRYLLDNYIYSPNEPKVPQIWIDFVVSGANQVADKVFEEEGIDICXXXXXXXXXXXXXXXDGYHVDSLYGGVPVGFIEYLEK-LHKEWC 2703
LP I + ++ ED L +I+ S FKL+ Y+L++ + L+ R + E VI + +E V +R+ + + I ++W+AN +E L+ +K + LS+ I D+ A Q F+ + D+ N + L DD EE + P S K D ++ TLS MS+L++C VN AL IQ+ S++FH IN W+FN + C W I+ ++ I W+E+ L LAADC+ + +QA LL M K E N +NS+QL LL NY + EP +P I+ VV+ A AD++ +G ++ L+ L LP LLPEDGY D + +P G ++LE L +++C
Sbjct: 560 LPASIEFRENSEDTFLSAIINYTNSSTVHFKLSPTYVLYMACRFV--LSPAYRPDISPSERTHKVIAIVNKMVSMMEGVIQDMREGDQKQKNIAGALAFWMANASELLNFIKQDRDLSR----ITLDAQDILAHLVQMAFKYLVHCLQADLNNYMPAFL----DDPEEHN--PQSPKIED------VLHTLSGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNKLVTDADSGLCCHY---WGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMNKYSKEDVQNIHSTCFKLNSLQLHALLTNYHCAQGEPYIPPELINEVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVR-NLPNGLQDFLEPLLQRDFC 931
BLAST of Afadin vs. Ensembl Medaka
Match: afdna (afadin, adherens junction formation factor [Source:NCBI gene;Acc:101169758]) HSP 1 Score: 158.688 bits (400), Expect = 1.449e-38 Identity = 121/358 (33.80%), Postives = 183/358 (51.12%), Query Frame = 1
Query: 121 LINQWNSSRLELFKISQPDKNLKFFGVMRFFFE--TEDKFHSKCLRVSSDLKVKDLLPILLEKFYVSDICSSKQDFRLFE-RSSSIDKLLDPEDFPLLIQLFWCKDDKEGVFVFK--SNLKNKNSRRIRSQTRKDKI----------------------------------GSTTCLNSPTTRCLDKVCTDPPTKSTKQXXXXXXXXXXXXXXXRTISNPXXXXXXXXXXXXXXXXXQ-ISKSGNLNGGGTLKIYGDSLNPLIPYKSLLLSIDDTAEFVVREILDKYGMENENENDFSLVSSVYKDQNTQKIEYEEILQNSDSPLRFLLQSNKNDGFIKFRVVKMPANISKSKKSKTQ 1074
+IN WN++RL+LF+ISQP ++L+F GVMRF+F+ F +KC+RVSS +D++ L EKF S + L+E S ++ LD ++ PL++QL W KDD+EG FV K +++ K S+ + KD + G NS R +V D P S +RTISNPE +M+ +R+ K+E++++ I+ G + GGTL+IY DSL P IPYK+LL S+ DTA+ VV E L+KYG+E EN +F + + +K E IL +S+ PL+ L + G + F++ K P + K K +
Sbjct: 15 IINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERQLDLDEKPLVVQLNWNKDDREGRFVLKNENDVPPKKSQSNGPEREKDGVIQNFKRTLSKKEKKKEKKREKEFIRMPDEDERTLGREDGENS---RLAAEVYKDMPETS----------------FTRTISNPEVVMKRRRQQKVEKRMQDFINNDGRPDSGGTLRIYADSLKPNIPYKTLLCSVRDTADLVVVEALEKYGLEKENPREFCIA------RQEEKTGKEVILDDSECPLQILRDWPADRGVLVFQLKKKPPDYQGRKGRKME 347 HSP 2 Score: 147.132 bits (370), Expect = 5.770e-35 Identity = 123/394 (31.22%), Postives = 192/394 (48.73%), Query Frame = 1
Query: 1567 LPVQITYFKSKEDMLLDSILLDIKPSQTKFKLASAYLLHLMVVDIIRLTCINRATVLDQEL---VIGM------FIEKVASKIRQSIMQHRTIISIQSYWLANCTEFLHLLKNNILLSQFNSSIFDKYYDLFADNAQWCFQCISFGFTDDMVNTLSVLLNENTDDSEEKDDFPLSRKASDPKNMSILIRTLSSCMSVLQQCHVNPALIIQIISRIFHLINCWIFNSFIDTP-----CRIFRGWAXXXXXXXXXXXXWSERHDLLLAADCYFQQSLQACKLLIMEKTDIEVFYNETVDMVDINSIQLRYLLDNYIYSPNEPKVPQIWIDFVVSGANQVADKVFEEEGIDICXXXXXXXXXXXXXXXDGYHVDSLYGGVPVGFIEYLEK-LHKEWC 2703
LP I + ++ ED L +I+ S FKL+ Y+L++ + L+ R + E VI + +E V +R+ + + I ++W+AN +E L+ +K + LS+ I D+ A Q F+ + D+ N + L DD EE + P S K D ++ TLS MS+L++C VN AL IQ+ S++FH IN W+FN + C W I+ ++ I W+E+ L LAADC+ + +QA LL M K E N +NS+QL LL NY + EP +P I+ VV+ A AD++ +G ++ L+ L LP LLPEDGY D + +P G ++LE L +++C
Sbjct: 545 LPASIEFRENSEDTFLSAIINYTNSSTVHFKLSPTYVLYMACRFV--LSPAYRPDISPSERTHKVIAIVNKMVSMMEGVIQDMREGDQKQKNIAGALAFWMANASELLNFIKQDRDLSR----ITLDAQDILAHLVQMAFKYLVHCLQADLNNYMPAFL----DDPEEHN--PQSPKIED------VLHTLSGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNKLVTDADSGLCCHY---WGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMNKYSKEDVQNIHSTCFKLNSLQLHALLTNYHCAQGEPYIPPELINEVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVR-NLPNGLQDFLEPLLQRDFC 916
BLAST of Afadin vs. Ensembl Medaka
Match: afdna (afadin, adherens junction formation factor [Source:NCBI gene;Acc:101169758]) HSP 1 Score: 158.688 bits (400), Expect = 1.517e-38 Identity = 121/358 (33.80%), Postives = 183/358 (51.12%), Query Frame = 1
Query: 121 LINQWNSSRLELFKISQPDKNLKFFGVMRFFFE--TEDKFHSKCLRVSSDLKVKDLLPILLEKFYVSDICSSKQDFRLFE-RSSSIDKLLDPEDFPLLIQLFWCKDDKEGVFVFK--SNLKNKNSRRIRSQTRKDKI----------------------------------GSTTCLNSPTTRCLDKVCTDPPTKSTKQXXXXXXXXXXXXXXXRTISNPXXXXXXXXXXXXXXXXXQ-ISKSGNLNGGGTLKIYGDSLNPLIPYKSLLLSIDDTAEFVVREILDKYGMENENENDFSLVSSVYKDQNTQKIEYEEILQNSDSPLRFLLQSNKNDGFIKFRVVKMPANISKSKKSKTQ 1074
+IN WN++RL+LF+ISQP ++L+F GVMRF+F+ F +KC+RVSS +D++ L EKF S + L+E S ++ LD ++ PL++QL W KDD+EG FV K +++ K S+ + KD + G NS R +V D P S +RTISNPE +M+ +R+ K+E++++ I+ G + GGTL+IY DSL P IPYK+LL S+ DTA+ VV E L+KYG+E EN +F + + +K E IL +S+ PL+ L + G + F++ K P + K K +
Sbjct: 15 IINHWNANRLDLFEISQPTEDLEFHGVMRFYFQDRVAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERQLDLDEKPLVVQLNWNKDDREGRFVLKNENDVPPKKSQSNGPEREKDGVIQNFKRTLSKKEKKKEKKREKEFIRMPDEDERTLGREDGENS---RLAAEVYKDMPETS----------------FTRTISNPEVVMKRRRQQKVEKRMQDFINNDGRPDSGGTLRIYADSLKPNIPYKTLLCSVRDTADLVVVEALEKYGLEKENPREFCIA------RQEEKTGKEVILDDSECPLQILRDWPADRGVLVFQLKKKPPDYQGRKGRKME 347 HSP 2 Score: 149.058 bits (375), Expect = 1.595e-35 Identity = 118/385 (30.65%), Postives = 189/385 (49.09%), Query Frame = 1
Query: 1567 LPVQITYFKSKEDMLLDSILLDIKPSQTKFKLASAYLLHLMVVDIIRLTCINRATVLDQELVIGMFIEKVASKIRQSIMQHRTIISIQSYWLANCTEFLHLLKNNILLSQFNSSIFDKYYDLFADNAQWCFQCISFGFTDDMVNTLSVLLNENTDDSEEKDDFPLSRKASDPKNMSILIRTLSSCMSVLQQCHVNPALIIQIISRIFHLINCWIFNSFIDTP-----CRIFRGWAXXXXXXXXXXXXWSERHDLLLAADCYFQQSLQACKLLIMEKTDIEVFYNETVDMVDINSIQLRYLLDNYIYSPNEPKVPQIWIDFVVSGANQVADKVFEEEGIDICXXXXXXXXXXXXXXXDGYHVDSLYGGVPVGFIEYLEK-LHKEWC 2703
LP I + ++ ED L +I+ S FKL+ Y+L++ ++ + ++ + + K+ S + I + + I ++W+AN +E L+ +K + LS+ I D+ A Q F+ + D+ N + L DD EE + P S K D ++ TLS MS+L++C VN AL IQ+ S++FH IN W+FN + C W I+ ++ I W+E+ L LAADC+ + +QA LL M K E N +NS+QL LL NY + EP +P I+ VV+ A AD++ +G ++ L+ L LP LLPEDGY D + +P G ++LE L +++C
Sbjct: 560 LPASIEFRENSEDTFLSAIINYTNSSTVHFKLSPTYVLYMACRFVLSPAYRPDISPSERTHKVIAIVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSR----ITLDAQDILAHLVQMAFKYLVHCLQADLNNYMPAFL----DDPEEHN--PQSPKIED------VLHTLSGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNKLVTDADSGLCCHY---WGAILRQQLSHIEAWAEKQGLELAADCHLSRIVQATTLLTMNKYSKEDVQNIHSTCFKLNSLQLHALLTNYHCAQGEPYIPPELINEVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVR-NLPNGLQDFLEPLLQRDFC 924
BLAST of Afadin vs. Planmine SMEST
Match: SMESG000058689.1 (SMESG000058689.1) HSP 1 Score: 2363.19 bits (6123), Expect = 0.000e+0 Identity = 1235/1243 (99.36%), Postives = 1239/1243 (99.68%), Query Frame = 1
Query: 103 MTTILHLINQWNSSRLELFKISQPDKNLKFFGVMRFFFETEDKFHSKCLRVSSDLKVKDLLPILLEKFYVSDICSSKQDFRLFERSSSIDKLLDPEDFPLLIQLFWCKDDKEGVFVFKSNLKNKNSRRIRSQTRKDKIGSTTCLNSPTTRCLDKVCTDPPTKSTKQXXXXXXXXXXXXXXXRTISNPXXXXXXXXXXXXXXXXXQISKSGNLNGGGTLKIYGDSLNPLIPYKSLLLSIDDTAEFVVREILDKYGMENENENDFSLVSSVYKDQNTQKIEYEEILQNSDSPLRFLLQSNKNDGFIKFRVVKMPANISKSKKSKTQGTFPDVCKSIAIRKSNSVNYRNSDVQSHSRNTCDGKFKHSLHPDFSNNYNGSNNKIYSTPPSSDSPVPLLKRVESEKKPLPTYEESVSALRSWRVKISNFPENHKPISYRGSTESFSLSSDYQEDSSYNTDSYESRNIPSVETPEQENDDVVIISDLDSHLSRHLPVQITYFKSKEDMLLDSILLDIKPSQTKFKLASAYLLHLMVVDIIRLTCINRATVLDQELVIGMFIEKVASKIRQSIMQHRTIISIQSYWLANCTEFLHLLKNNILLSQFNSSIFDKYYDLFADNAQWCFQCISFGFTDDMVNTLSVLLNENTDDSEEKDDFPLSRKASDPKNMSILIRTLSSCMSVLQQCHVNPALIIQIISRIFHLINCWIFNSFIDTPCRIFRGWAXXXXXXXXXXXXWSERHDLLLAADCYFQQSLQACKLLIMEKTDIEVFYNETVDMVDINSIQLRYLLDNYIYSPNEPKVPQIWIDFVVSGANQVADKVFEEEGIDICXXXXXXXXXXXXXXXDGYHVDSLYGGVPVGFIEYLEKLHKEWCFYKISHQDIHFDWLCQKRGFQDTSCELLPFENCVKVEDNLNKSNSSNRHQLKLSDFKANVYYENLDTINNNESTGESGIDTYRNQSYQHSASRLDVNDNQCARKKIQCTDLPLNFTYDLYQNNCPKPFSDAGSTTAASLLSEIIDDDNPCNSHSKRHRVIKDLRKSLENSIEKTIVAQEQFQSVIRGTDEIYTKELIKRLDKLEKDKSEEIYKMNYQQFHRVQSTNLSLENLENKRQTDYIKQSERMKNICELESQMESIDKYSFLKSSTLPIRNSNSGSQPPASFVPASLALRNSLNYPPRLRPKPKIHSLSTTNRSHSAANXXXXXXXXXXXXXXPPRSLSNDRFYSRMSDGMENLTFQEKVQLFEFAISRSSH 3831
MTTILHLINQWNSSRLELFKISQPDKNLKFFGVMRFFFETEDKFHSKCLRVSSDLKVKDLLP+LLEKFYVSDICSSKQDFRLFERSSSIDKLLDPEDFPLLIQLFWCKDDKEGVFVFKSNLKNKNSR IRSQTRK KIGSTTCLNSPTTRCLDKVCTDPPTKSTKQFSKSPSLLSTPSFLSRTISNPESLMRLKRKSKLEEKLKQISKSGNLNGGGTLKIYGDSLNPLIPYKSLLLSIDDTAEFVVREILDKYGMENENENDFSLVSSVYKDQNTQKIEYEEILQNSDSPLRFLLQSNKNDGFIKFRVVKMPANISKSKKSKTQGTFPDVCKSIAIRKSNSVNYRNSDVQSHSRNTCDGKFKHSLHPDFSNNYNGSNNKIYSTPPSSDSPVPLLKRVESEKKPLPTYEESVSALRSWRVKISNFPENHKPISYRGSTESFSLSSDYQEDSSYNTDS+ESRNIPSVETPEQENDDVVIISDLDSHLSRHLPVQITYFKSKEDMLLDSILLDIKPSQTKFKLASAY+LHLMVVDIIRLTCINRATVLDQELVIGMFIEKVASKIRQSIMQHRTIISIQSYWLANCTEFLHLLKNNILLSQFNSSIFDKYYDLFADNAQWCFQCISFGFTDDMVNTLSVLLNENTDDSEEKDDFPLSRKASDPKNMSILIRTLSSCMSVLQQCHVNPALIIQIISRIFHLINCWIFNSFIDTPCRIFRGWARIILNRIERIRRWSERHDLLLAADCYFQQSLQACKLLIMEKTDIEVFYNETVDMVDINSIQLRYLLDNYIYSPNEPKVPQIWIDFVVSGANQVADKVFEEEGIDICLKLKESLNLPLLLPEDGYHVDSLYGGVPVGFIEYLEKLHKEWCFYKISHQDIHFDWLCQKRGFQDTSCELLPFENCVKVEDNLNKSNSSNRHQLKLSDFKANVYYENLDTINNNESTGESGIDTYRNQSYQHSASRLDVNDNQCARKKIQCTDLPLNFTYDLYQNNCPKPFSDAGSTTAASLLSEIIDDDNPCNSHSKRHRVIKDLRKSLE+SIEKTIVAQEQFQSVI GTDEIYTKELIKRLDKLEKDKSEEIYKMNYQQFHRVQSTNLSLENLENKRQTDYI QSERMKNICELESQMESIDKYSFLKSSTLPIRNSNSGSQPPASFVPASLALRNSLNYPPRLRPKPKIHSLSTTNRSHSAANISKSDSSFEISSRIPPRSLSNDRFYSRMSDGMENLTFQEKVQLFEFAISRSSH
Sbjct: 1 MTTILHLINQWNSSRLELFKISQPDKNLKFFGVMRFFFETEDKFHSKCLRVSSDLKVKDLLPVLLEKFYVSDICSSKQDFRLFERSSSIDKLLDPEDFPLLIQLFWCKDDKEGVFVFKSNLKNKNSRWIRSQTRKYKIGSTTCLNSPTTRCLDKVCTDPPTKSTKQFSKSPSLLSTPSFLSRTISNPESLMRLKRKSKLEEKLKQISKSGNLNGGGTLKIYGDSLNPLIPYKSLLLSIDDTAEFVVREILDKYGMENENENDFSLVSSVYKDQNTQKIEYEEILQNSDSPLRFLLQSNKNDGFIKFRVVKMPANISKSKKSKTQGTFPDVCKSIAIRKSNSVNYRNSDVQSHSRNTCDGKFKHSLHPDFSNNYNGSNNKIYSTPPSSDSPVPLLKRVESEKKPLPTYEESVSALRSWRVKISNFPENHKPISYRGSTESFSLSSDYQEDSSYNTDSFESRNIPSVETPEQENDDVVIISDLDSHLSRHLPVQITYFKSKEDMLLDSILLDIKPSQTKFKLASAYVLHLMVVDIIRLTCINRATVLDQELVIGMFIEKVASKIRQSIMQHRTIISIQSYWLANCTEFLHLLKNNILLSQFNSSIFDKYYDLFADNAQWCFQCISFGFTDDMVNTLSVLLNENTDDSEEKDDFPLSRKASDPKNMSILIRTLSSCMSVLQQCHVNPALIIQIISRIFHLINCWIFNSFIDTPCRIFRGWARIILNRIERIRRWSERHDLLLAADCYFQQSLQACKLLIMEKTDIEVFYNETVDMVDINSIQLRYLLDNYIYSPNEPKVPQIWIDFVVSGANQVADKVFEEEGIDICLKLKESLNLPLLLPEDGYHVDSLYGGVPVGFIEYLEKLHKEWCFYKISHQDIHFDWLCQKRGFQDTSCELLPFENCVKVEDNLNKSNSSNRHQLKLSDFKANVYYENLDTINNNESTGESGIDTYRNQSYQHSASRLDVNDNQCARKKIQCTDLPLNFTYDLYQNNCPKPFSDAGSTTAASLLSEIIDDDNPCNSHSKRHRVIKDLRKSLEDSIEKTIVAQEQFQSVIHGTDEIYTKELIKRLDKLEKDKSEEIYKMNYQQFHRVQSTNLSLENLENKRQTDYIIQSERMKNICELESQMESIDKYSFLKSSTLPIRNSNSGSQPPASFVPASLALRNSLNYPPRLRPKPKIHSLSTTNRSHSAANISKSDSSFEISSRIPPRSLSNDRFYSRMSDGMENLTFQEKVQLFEFAISRSSH 1243
BLAST of Afadin vs. Planmine SMEST
Match: SMESG000058689.1 (SMESG000058689.1) HSP 1 Score: 1543.1 bits (3994), Expect = 0.000e+0 Identity = 805/812 (99.14%), Postives = 809/812 (99.63%), Query Frame = 1
Query: 103 MTTILHLINQWNSSRLELFKISQPDKNLKFFGVMRFFFETEDKFHSKCLRVSSDLKVKDLLPILLEKFYVSDICSSKQDFRLFERSSSIDKLLDPEDFPLLIQLFWCKDDKEGVFVFKSNLKNKNSRRIRSQTRKDKIGSTTCLNSPTTRCLDKVCTDPPTKSTKQXXXXXXXXXXXXXXXRTISNPXXXXXXXXXXXXXXXXXQISKSGNLNGGGTLKIYGDSLNPLIPYKSLLLSIDDTAEFVVREILDKYGMENENENDFSLVSSVYKDQNTQKIEYEEILQNSDSPLRFLLQSNKNDGFIKFRVVKMPANISKSKKSKTQGTFPDVCKSIAIRKSNSVNYRNSDVQSHSRNTCDGKFKHSLHPDFSNNYNGSNNKIYSTPPSSDSPVPLLKRVESEKKPLPTYEESVSALRSWRVKISNFPENHKPISYRGSTESFSLSSDYQEDSSYNTDSYESRNIPSVETPEQENDDVVIISDLDSHLSRHLPVQITYFKSKEDMLLDSILLDIKPSQTKFKLASAYLLHLMVVDIIRLTCINRATVLDQELVIGMFIEKVASKIRQSIMQHRTIISIQSYWLANCTEFLHLLKNNILLSQFNSSIFDKYYDLFADNAQWCFQCISFGFTDDMVNTLSVLLNENTDDSEEKDDFPLSRKASDPKNMSILIRTLSSCMSVLQQCHVNPALIIQIISRIFHLINCWIFNSFIDTPCRIFRGWAXXXXXXXXXXXXWSERHDLLLAADCYFQQSLQACKLLIMEKTDIEVFYNETVDMVDINSIQLRYLLDNYIYSPNEPKVPQIWIDFVVSGANQVA 2538
MTTILHLINQWNSSRLELFKISQPDKNLKFFGVMRFFFETEDKFHSKCLRVSSDLKVKDLLP+LLEKFYVSDICSSKQDFRLFERSSSIDKLLDPEDFPLLIQLFWCKDDKEGVFVFKSNLKNKNSR IRSQTRK KIGSTTCLNSPTTRCLDKVCTDPPTKSTKQFSKSPSLLSTPSFLSRTISNPESLMRLKRKSKLEEKLKQISKSGNLNGGGTLKIYGDSLNPLIPYKSLLLSIDDTAEFVVREILDKYGMENENENDFSLVSSVYKDQNTQKIEYEEILQNSDSPLRFLLQSNKNDGFIKFRVVKMPANISKSKKSKTQGTFPDVCKSIAIRKSNSVNYRNSDVQSHSRNTCDGKFKHSLHPDFSNNYNGSNNKIYSTPPSSDSPVPLLKRVESEKKPLPTYEESVSALRSWRVKISNFPENHKPISYRGSTESFSLSSDYQEDSSYNTDS+ESRNIPSVETPEQENDDVVIISDLDSHLSRHLPVQITYFKSKEDMLLDSILLDIKPSQTKFKLASAY+LHLMVVDIIRLTCINRATVLDQELVIGMFIEKVASKIRQSIMQHRTIISIQSYWLANCTEFLHLLKNNILLSQFNSSIFDKYYDLFADNAQWCFQCISFGFTDDMVNTLSVLLNENTDDSEEKDDFPLSRKASDPKNMSILIRTLSSCMSVLQQCHVNPALIIQIISRIFHLINCWIFNSFIDTPCRIFRGWARIILNRIERIRRWSERHDLLLAADCYFQQSLQACKLLIMEKTDIEVFYNETVDMVDINSIQLRYLLDNYIYSPNEPKVPQIWIDFVVSGANQ +
Sbjct: 1 MTTILHLINQWNSSRLELFKISQPDKNLKFFGVMRFFFETEDKFHSKCLRVSSDLKVKDLLPVLLEKFYVSDICSSKQDFRLFERSSSIDKLLDPEDFPLLIQLFWCKDDKEGVFVFKSNLKNKNSRWIRSQTRKYKIGSTTCLNSPTTRCLDKVCTDPPTKSTKQFSKSPSLLSTPSFLSRTISNPESLMRLKRKSKLEEKLKQISKSGNLNGGGTLKIYGDSLNPLIPYKSLLLSIDDTAEFVVREILDKYGMENENENDFSLVSSVYKDQNTQKIEYEEILQNSDSPLRFLLQSNKNDGFIKFRVVKMPANISKSKKSKTQGTFPDVCKSIAIRKSNSVNYRNSDVQSHSRNTCDGKFKHSLHPDFSNNYNGSNNKIYSTPPSSDSPVPLLKRVESEKKPLPTYEESVSALRSWRVKISNFPENHKPISYRGSTESFSLSSDYQEDSSYNTDSFESRNIPSVETPEQENDDVVIISDLDSHLSRHLPVQITYFKSKEDMLLDSILLDIKPSQTKFKLASAYVLHLMVVDIIRLTCINRATVLDQELVIGMFIEKVASKIRQSIMQHRTIISIQSYWLANCTEFLHLLKNNILLSQFNSSIFDKYYDLFADNAQWCFQCISFGFTDDMVNTLSVLLNENTDDSEEKDDFPLSRKASDPKNMSILIRTLSSCMSVLQQCHVNPALIIQIISRIFHLINCWIFNSFIDTPCRIFRGWARIILNRIERIRRWSERHDLLLAADCYFQQSLQACKLLIMEKTDIEVFYNETVDMVDINSIQLRYLLDNYIYSPNEPKVPQIWIDFVVSGANQAS 812
BLAST of Afadin vs. Planmine SMEST
Match: SMESG000058689.1 (SMESG000058689.1) HSP 1 Score: 1542.71 bits (3993), Expect = 0.000e+0 Identity = 806/811 (99.38%), Postives = 809/811 (99.75%), Query Frame = 1
Query: 103 MTTILHLINQWNSSRLELFKISQPDKNLKFFGVMRFFFETEDKFHSKCLRVSSDLKVKDLLPILLEKFYVSDICSSKQDFRLFERSSSIDKLLDPEDFPLLIQLFWCKDDKEGVFVFKSNLKNKNSRRIRSQTRKDKIGSTTCLNSPTTRCLDKVCTDPPTKSTKQXXXXXXXXXXXXXXXRTISNPXXXXXXXXXXXXXXXXXQISKSGNLNGGGTLKIYGDSLNPLIPYKSLLLSIDDTAEFVVREILDKYGMENENENDFSLVSSVYKDQNTQKIEYEEILQNSDSPLRFLLQSNKNDGFIKFRVVKMPANISKSKKSKTQGTFPDVCKSIAIRKSNSVNYRNSDVQSHSRNTCDGKFKHSLHPDFSNNYNGSNNKIYSTPPSSDSPVPLLKRVESEKKPLPTYEESVSALRSWRVKISNFPENHKPISYRGSTESFSLSSDYQEDSSYNTDSYESRNIPSVETPEQENDDVVIISDLDSHLSRHLPVQITYFKSKEDMLLDSILLDIKPSQTKFKLASAYLLHLMVVDIIRLTCINRATVLDQELVIGMFIEKVASKIRQSIMQHRTIISIQSYWLANCTEFLHLLKNNILLSQFNSSIFDKYYDLFADNAQWCFQCISFGFTDDMVNTLSVLLNENTDDSEEKDDFPLSRKASDPKNMSILIRTLSSCMSVLQQCHVNPALIIQIISRIFHLINCWIFNSFIDTPCRIFRGWAXXXXXXXXXXXXWSERHDLLLAADCYFQQSLQACKLLIMEKTDIEVFYNETVDMVDINSIQLRYLLDNYIYSPNEPKVPQIWIDFVVSGANQV 2535
MTTILHLINQWNSSRLELFKISQPDKNLKFFGVMRFFFETEDKFHSKCLRVSSDLKVKDLLP+LLEKFYVSDICSSKQDFRLFERSSSIDKLLDPEDFPLLIQLFWCKDDKEGVFVFKSNLKNKNSR IRSQTRK KIGSTTCLNSPTTRCLDKVCTDPPTKSTKQFSKSPSLLSTPSFLSRTISNPESLMRLKRKSKLEEKLKQISKSGNLNGGGTLKIYGDSLNPLIPYKSLLLSIDDTAEFVVREILDKYGMENENENDFSLVSSVYKDQNTQKIEYEEILQNSDSPLRFLLQSNKNDGFIKFRVVKMPANISKSKKSKTQGTFPDVCKSIAIRKSNSVNYRNSDVQSHSRNTCDGKFKHSLHPDFSNNYNGSNNKIYSTPPSSDSPVPLLKRVESEKKPLPTYEESVSALRSWRVKISNFPENHKPISYRGSTESFSLSSDYQEDSSYNTDS+ESRNIPSVETPEQENDDVVIISDLDSHLSRHLPVQITYFKSKEDMLLDSILLDIKPSQTKFKLASAY+LHLMVVDIIRLTCINRATVLDQELVIGMFIEKVASKIRQSIMQHRTIISIQSYWLANCTEFLHLLKNNILLSQFNSSIFDKYYDLFADNAQWCFQCISFGFTDDMVNTLSVLLNENTDDSEEKDDFPLSRKASDPKNMSILIRTLSSCMSVLQQCHVNPALIIQIISRIFHLINCWIFNSFIDTPCRIFRGWARIILNRIERIRRWSERHDLLLAADCYFQQSLQACKLLIMEKTDIEVFYNETVDMVDINSIQLRYLLDNYIYSPNEPKVPQIWIDFVVSGANQV
Sbjct: 1 MTTILHLINQWNSSRLELFKISQPDKNLKFFGVMRFFFETEDKFHSKCLRVSSDLKVKDLLPVLLEKFYVSDICSSKQDFRLFERSSSIDKLLDPEDFPLLIQLFWCKDDKEGVFVFKSNLKNKNSRWIRSQTRKYKIGSTTCLNSPTTRCLDKVCTDPPTKSTKQFSKSPSLLSTPSFLSRTISNPESLMRLKRKSKLEEKLKQISKSGNLNGGGTLKIYGDSLNPLIPYKSLLLSIDDTAEFVVREILDKYGMENENENDFSLVSSVYKDQNTQKIEYEEILQNSDSPLRFLLQSNKNDGFIKFRVVKMPANISKSKKSKTQGTFPDVCKSIAIRKSNSVNYRNSDVQSHSRNTCDGKFKHSLHPDFSNNYNGSNNKIYSTPPSSDSPVPLLKRVESEKKPLPTYEESVSALRSWRVKISNFPENHKPISYRGSTESFSLSSDYQEDSSYNTDSFESRNIPSVETPEQENDDVVIISDLDSHLSRHLPVQITYFKSKEDMLLDSILLDIKPSQTKFKLASAYVLHLMVVDIIRLTCINRATVLDQELVIGMFIEKVASKIRQSIMQHRTIISIQSYWLANCTEFLHLLKNNILLSQFNSSIFDKYYDLFADNAQWCFQCISFGFTDDMVNTLSVLLNENTDDSEEKDDFPLSRKASDPKNMSILIRTLSSCMSVLQQCHVNPALIIQIISRIFHLINCWIFNSFIDTPCRIFRGWARIILNRIERIRRWSERHDLLLAADCYFQQSLQACKLLIMEKTDIEVFYNETVDMVDINSIQLRYLLDNYIYSPNEPKVPQIWIDFVVSGANQV 811
BLAST of Afadin vs. Planmine SMEST
Match: SMESG000058689.1 (SMESG000058689.1) HSP 1 Score: 827.395 bits (2136), Expect = 0.000e+0 Identity = 433/440 (98.41%), Postives = 435/440 (98.86%), Query Frame = 1
Query: 2512 VVSGANQVADKVFEEEGIDICXXXXXXXXXXXXXXXDGYHVDSLYGGVPVGFIEYLEKLHKEWCFYKISHQDIHFDWLCQKRGFQDTSCELLPFENCVKVEDNLNKSNSSNRHQLKLSDFKANVYYENLDTINNNESTGESGIDTYRNQSYQHSASRLDVNDNQCARKKIQCTDLPLNFTYDLYQNNCPKPFSDAGSTTAASLLSEIIDDDNPCNSHSKRHRVIKDLRKSLENSIEKTIVAQEQFQSVIRGTDEIYTKELIKRLDKLEKDKSEEIYKMNYQQFHRVQSTNLSLENLENKRQTDYIKQSERMKNICELESQMESIDKYSFLKSSTLPIRNSNSGSQPPASFVPASLALRNSLNYPPRLRPKPKIHSLSTTNRSHSAANXXXXXXXXXXXXXXPPRSLSNDRFYSRMSDGMENLTFQEKVQLFEFAISRSSH 3831
V+S A VADKVFEEEGIDICLKLKESLNLPLLLPEDGYHVDSLYGGVPVGFIEYLEKLHKEWCFYKISHQDIHFDWLCQKRGFQDTSCELLPFENCVKVEDNLNKSNSSNRHQLKLSDFKANVYYENLDTINNNESTGESGIDTYRNQSYQHSASRLDVNDNQCARKKIQCTDLPLNFTYDLYQNNCPKPFSDAGSTTAASLLSEIIDDDNPCNSHSKRHRVIKDLRKSLE+SIEKTIVAQEQFQSVI GTDEIYTKELIKRLDKLEKDKSEEIYKMNYQQFHRVQSTNLSLENLENKRQTDYI QSERMKNICELESQMESIDKYSFLKSSTLPIRNSNSGSQPPASFVPASLALRNSLNYPPRLRPKPKIHSLSTTNRSHSAANISKSDSSFEISSRIPPRSLSNDRFYSRMSDGMENLTFQEKVQLFEFAISRSSH
Sbjct: 120 VLSVALHVADKVFEEEGIDICLKLKESLNLPLLLPEDGYHVDSLYGGVPVGFIEYLEKLHKEWCFYKISHQDIHFDWLCQKRGFQDTSCELLPFENCVKVEDNLNKSNSSNRHQLKLSDFKANVYYENLDTINNNESTGESGIDTYRNQSYQHSASRLDVNDNQCARKKIQCTDLPLNFTYDLYQNNCPKPFSDAGSTTAASLLSEIIDDDNPCNSHSKRHRVIKDLRKSLEDSIEKTIVAQEQFQSVIHGTDEIYTKELIKRLDKLEKDKSEEIYKMNYQQFHRVQSTNLSLENLENKRQTDYIIQSERMKNICELESQMESIDKYSFLKSSTLPIRNSNSGSQPPASFVPASLALRNSLNYPPRLRPKPKIHSLSTTNRSHSAANISKSDSSFEISSRIPPRSLSNDRFYSRMSDGMENLTFQEKVQLFEFAISRSSH 559
BLAST of Afadin vs. Planmine SMEST
Match: SMESG000058689.1 (SMESG000058689.1) HSP 1 Score: 820.846 bits (2119), Expect = 0.000e+0 Identity = 430/434 (99.08%), Postives = 432/434 (99.54%), Query Frame = 1
Query: 2530 QVADKVFEEEGIDICXXXXXXXXXXXXXXXDGYHVDSLYGGVPVGFIEYLEKLHKEWCFYKISHQDIHFDWLCQKRGFQDTSCELLPFENCVKVEDNLNKSNSSNRHQLKLSDFKANVYYENLDTINNNESTGESGIDTYRNQSYQHSASRLDVNDNQCARKKIQCTDLPLNFTYDLYQNNCPKPFSDAGSTTAASLLSEIIDDDNPCNSHSKRHRVIKDLRKSLENSIEKTIVAQEQFQSVIRGTDEIYTKELIKRLDKLEKDKSEEIYKMNYQQFHRVQSTNLSLENLENKRQTDYIKQSERMKNICELESQMESIDKYSFLKSSTLPIRNSNSGSQPPASFVPASLALRNSLNYPPRLRPKPKIHSLSTTNRSHSAANXXXXXXXXXXXXXXPPRSLSNDRFYSRMSDGMENLTFQEKVQLFEFAISRSSH 3831
+VADKVFEEEGIDICLKLKESLNLPLLLPEDGYHVDSLYGGVPVGFIEYLEKLHKEWCFYKISHQDIHFDWLCQKRGFQDTSCELLPFENCVKVEDNLNKSNSSNRHQLKLSDFKANVYYENLDTINNNESTGESGIDTYRNQSYQHSASRLDVNDNQCARKKIQCTDLPLNFTYDLYQNNCPKPFSDAGSTTAASLLSEIIDDDNPCNSHSKRHRVIKDLRKSLE+SIEKTIVAQEQFQSVI GTDEIYTKELIKRLDKLEKDKSEEIYKMNYQQFHRVQSTNLSLENLENKRQTDYI QSERMKNICELESQMESIDKYSFLKSSTLPIRNSNSGSQPPASFVPASLALRNSLNYPPRLRPKPKIHSLSTTNRSHSAANISKSDSSFEISSRIPPRSLSNDRFYSRMSDGMENLTFQEKVQLFEFAISRSSH
Sbjct: 5 KVADKVFEEEGIDICLKLKESLNLPLLLPEDGYHVDSLYGGVPVGFIEYLEKLHKEWCFYKISHQDIHFDWLCQKRGFQDTSCELLPFENCVKVEDNLNKSNSSNRHQLKLSDFKANVYYENLDTINNNESTGESGIDTYRNQSYQHSASRLDVNDNQCARKKIQCTDLPLNFTYDLYQNNCPKPFSDAGSTTAASLLSEIIDDDNPCNSHSKRHRVIKDLRKSLEDSIEKTIVAQEQFQSVIHGTDEIYTKELIKRLDKLEKDKSEEIYKMNYQQFHRVQSTNLSLENLENKRQTDYIIQSERMKNICELESQMESIDKYSFLKSSTLPIRNSNSGSQPPASFVPASLALRNSLNYPPRLRPKPKIHSLSTTNRSHSAANISKSDSSFEISSRIPPRSLSNDRFYSRMSDGMENLTFQEKVQLFEFAISRSSH 438 The following BLAST results are available for this feature:
BLAST of Afadin vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Afadin vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Afadin vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Afadin vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 2
BLAST of Afadin vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Afadin vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Afadin vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 3
BLAST of Afadin vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Afadin vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Afadin vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 1
BLAST of Afadin vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Afadin vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 1
BLAST of Afadin vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 3
BLAST of Afadin vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30019772 ID=SMED30019772|Name=Afadin|organism=Schmidtea mediterranea sexual|type=transcript|length=4202bpback to top protein sequence of SMED30019772-orf-1 >SMED30019772-orf-1 ID=SMED30019772-orf-1|Name=SMED30019772-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1244bp MTTILHLINQWNSSRLELFKISQPDKNLKFFGVMRFFFETEDKFHSKCLRback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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