SMED30019743

Overview
NameSMED30019743
Smed IDSMED30019743
Length (bp)1328
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30019743 (SMED30019743) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30019743 (SMED30019743) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30019743 (SMED30019743) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30019743 (SMED30019743) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30019743

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X2 cellSMED30019743h1SMcG0022291 SmedASXL_006137SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
epidermisSMED30019743h1SMcG0022291 dd_Smed_v4_11298_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30019743h1SMcG0022291 dd_Smed_v4_11298_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30019743h1SMcG0022291 dd_Smed_v4_11298_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30019743h1SMcG0022291 SMED30019743smed_20140614PMID:26457503
Tu et al., 2015		whole organism asexual adult colorimetric in situ hybridization evidence
parenchymaSMED30019743h1SMcG0022291 SMED30019743smed_20140614PMID:26457503
Tu et al., 2015		whole organism asexual adult colorimetric in situ hybridization evidence
Note: Hover over icons to view figure legend
Alignments
SMED30019743 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.002995:37973..40691 +6518
Homology
[Close]
BLAST of SMED30019743 vs. TrEMBL
Match: A0A0L8FFU7 (Uncharacterized protein OS=Octopus bimaculoides OX=37653 GN=OCBIM_22024326mg PE=4 SV=1)

HSP 1 Score: 110.153 bits (274), Expect = 4.743e-23
Identity = 80/248 (32.26%), Postives = 139/248 (56.05%), Query Frame = 2 
Query:  458 QQKLLHFKRRLSSEKKEKMLDLFRVFIRQMELYNLTYMAYGGTLIGINRHHGXXXXXXXXXXXXXAAQKRRVEKVMGKLHKTHKLYVVDESLHIKWKFFNINGLYTEGYDFKYPYIDIFSMKKISXXXXXXXXXXXSNMDL--HDIYPLVKRPFNGLTVYSPCN-VKKILKSTYTDFNKCTSSYYSHEFEDYLRG-NMIEVNCRMLKEHYPFVKKNFIAEENKIVLTERLIKNGTVLQIAKYSMPDAC 1189
            +  L  +K+++S    + + ++ +VF +  +  NLTY  Y G+L+G  RHHGIIPWDDD+DI+++++QK ++ + + K   T+ LY+   +   +WKF+  N        F++PYIDIF  ++  E   +     +S+  +    I+PL +RPFNG  ++SPCN V+ +L   + D  KC +S Y+H+ E++       +V C  L + +PFV +  I    + VL    ++  TV+      +PD+C
Sbjct:  116 RHSLAEYKQKMSLTDLKALNEMLQVFTKAADKANLTYFLYAGSLLGSYRHHGIIPWDDDVDIMMNSSQKGKIIETLKKFSPTYGLYIHPRN---QWKFYYANTNTLLHKPFRWPYIDIFFFQEDDEYIWDEIPTFKSSYTIKKSSIFPLQRRPFNGFMLWSPCNPVENLL---FYDIEKCAASSYNHDIEEWKPVYTWAKVQCTQLMDTFPFVWRIKITNGWEEVLWNSSVRQSTVI------VPDSC 351
[Close]
BLAST of SMED30019743 vs. TrEMBL
Match: R7T393 (Uncharacterized protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_192260 PE=4 SV=1)

HSP 1 Score: 110.153 bits (274), Expect = 1.279e-22
Identity = 77/210 (36.67%), Postives = 113/210 (53.81%), Query Frame = 2 
Query:  476 FKRRLSSEKKEKMLDLFRVFIRQMELYNLTYMAYGGTLIGINRHHGXXXXXXXXXXXXXAAQKRRVEKVMGKLHKTHKLYVVDESLHIKWKFFNINGLYTEGYDFKYPYIDIFSMKKISXXXXXXXXXXXSNMDLHD--IYPLVKRPFNGLTVYSPCNVKKILKSTYTDFNKCTSSYYSHEFEDYLRGNMIEVNCRMLKEHYPFVKKNFI 1099
            FKR +S  + + + DL  VF   M   NLTY    GTL+G  RHHGIIPWDDD+D+ I + QK RV + +  L   H L+       ++WK F+I     + Y + +P++DI  +   S+   + +    S        IYPL  RPF  L V++P N +++LK +Y D N C SS Y H  E +  G+ I + C  L + Y FVK++ +
Sbjct:  191 FKRAMSPGEYQAIQDLLSVFHSAMTKKNLTYFIAAGTLLGSYRHHGIIPWDDDVDVYILSDQKMRVMQALNSLGSEHALHTKT----VRWKLFSILSQPIQDYSWNFPFVDICMLDDYSDLLADSDPAFASRFSFPKDWIYPLRLRPFGRLMVWAPKNTERVLKQSY-DINICASSKYDHREEKFTAGS-ISIGCDFLNKTYLFVKRDRV 394
[Close]
BLAST of SMED30019743 vs. TrEMBL
Match: A0A0L8GPU7 (Uncharacterized protein OS=Octopus bimaculoides OX=37653 GN=OCBIM_22030157mg PE=4 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 9.094e-20
Identity = 72/240 (30.00%), Postives = 130/240 (54.17%), Query Frame = 2 
Query:  476 FKRRLSSEKKEKMLDLFRVFIRQMELYNLTYMAYGGTLIGINRHHGXXXXXXXXXXXXXAAQKRRVEKVMGKLHKTHKLYVVDESLHIKWKFFNINGLYTEGYDFKYPYIDIFSMKKISXXXXXXXXXXXSN--MDLHDIYPLVKRPFNGLTVYSPCNVKKILKSTYTDFNKCTSSYYSHEFEDYLRGNMIEVNCRMLKEHYPFVKKNFIAEENKIVLTERLIKNGTVLQIAKYSMPDAC 1189
            F+ R+++ +   +L L R+F +  +  NL Y  YGG+L+G  RHHG IPWDDDID++++++QK  + + +      +KLY      H +WKF+  +    +   F++PYIDIF  ++ +    +     +     D   ++PL KRPF+ L++ +PC+ +  L +   D   C ++ +SH+ E +L     ++ C  L++ +P V++     +     TE ++ NG++     YS P AC
Sbjct:   49 FQPRVTATEYRLLLTLTRIFAKAADTENLVYFLYGGSLLGSYRHHGFIPWDDDIDVIMNSSQKEEIRRALSAYEPKYKLYAPP---HKQWKFYRADSEALKEKPFRWPYIDIFFFQEDATHIWDEFFWYQFTYVYDKKRVFPLQKRPFDNLSLPAPCDTESFLGTYAADM--CRTNAFSHKEERWLY-RQAQLPCYQLRDSFPLVQRT----KTDDGWTE-VLWNGSIAVQYFYS-PHAC 276
[Close]
BLAST of SMED30019743 vs. TrEMBL
Match: R7T6X9 (Uncharacterized protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_213888 PE=4 SV=1)

HSP 1 Score: 100.138 bits (248), Expect = 1.700e-19
Identity = 73/207 (35.27%), Postives = 114/207 (55.07%), Query Frame = 2 
Query:  476 FKRRLSSEKKEKMLDLFRVFIRQMELYNLTYMAYGGTLIGINRHHGXXXXXXXXXXXXXAAQKRRVEKVMGKLHKTHKLYVVDESLHIKWKFFNINGLYTEGYDFKYPYIDIFSMKKISXXXXXXXXXXXSNMDLHD--IYPLVKRPFNGLTVYSPCNVKKILKSTYTDFNKCTSSYYSHEFEDYLRGNMIEVNCRMLKEHYPFVKK 1090
            F R +S ++ + + DL  VF   M   NLTY    GTL+G  RHHGIIPWDDD+D+ I + QK RV + +  L   H L    ++   +WK F++     +G+ +K+P++DI  +   S+   + +         H   I+PL  RPF  L V++P N + +LK +Y D + C S  Y+H  E +  G+++ ++C +LK  Y F KK
Sbjct:  165 FNRAMSRDEYQAIEDLLSVFHSAMTKNNLTYFIASGTLLGSYRHHGIIPWDDDVDVYILSDQKMRVMQALSSLGPQHAL----DAKTKRWKLFSVFSQPIQGHSWKFPFVDISLLDDHSDWLADSDPAFVPRFSFHKDWIFPLRLRPFGRLMVWAPKNTEMVLKKSY-DISICASLNYNHREEIFTAGSIM-ISCDVLKRIYTFCKK 365
[Close]
BLAST of SMED30019743 vs. TrEMBL
Match: A0A3S1B5A0 (Uncharacterized protein (Fragment) OS=Elysia chlorotica OX=188477 GN=EGW08_011999 PE=4 SV=1)

HSP 1 Score: 96.6709 bits (239), Expect = 3.909e-19
Identity = 73/226 (32.30%), Postives = 123/226 (54.42%), Query Frame = 2 
Query:  476 FKRRLSSEKKEKMLDLFRVFIRQMELYNLTYMAYGGTLIGINRHHGXXXXXXXXXXXXXAAQKRRVEKVMGKLHKTHKLYVVDESLHIKWKFFNINGLYTEGYDFKYPYIDIFSMKKISXXXXXXXXXXXSNMDLH--DIYPLVKRPFNGLTVYSPCNVKKILKSTYTDFNKCTSSYYSHEFEDY-LRGNMIEVNCRMLKEHYPFVKK------NFIAEENKIVLT 1126
            F+  ++ E+K+ +L +F+ F+R  + +N+T+  YGGTL+G  RHH +IPWDDDID+ + A +K  + + +  L+ T   Y++ + L   WKF+           F++PY+DIF  +  +    + + E  ++      D++PL  RPF G  +  PCN  ++L+  Y+  N C+S  +SH  E     G  + + C+ L + YPFV +      N + EE KI  T
Sbjct:    1 FRPIITVEEKKLLLLVFQKFVRACKDFNVTFFLYGGTLLGSFRHHDLIPWDDDIDVFVPAREKHILRRALSPLNYTG--YLLYQPLDKPWKFYWNKTKTLLHKPFRWPYVDIFFYEDNATHIFDQQIEYRASFAYRKVDVFPLTVRPFAGAFLPVPCNTDRVLRQNYSP-NLCSSQRFSHRTETLPAWGPHLIIPCKRLHDVYPFVHRQWSHTGNLVTEEVKIGAT 223
[Close]
BLAST of SMED30019743 vs. Planmine SMEST
Match: SMESG000023088.1 (SMESG000023088.1)

HSP 1 Score: 724.161 bits (1868), Expect = 0.000e+0
Identity = 378/383 (98.69%), Postives = 381/383 (99.48%), Query Frame = 2 
Query:   95 MIVKMKRKFLYLLLLCVSVAICLFIADLERNKYFPPLSEHLVLQEFQKFKISDCSIDQKIPSAVNVQSTVTYDYVNELIGKVNDSVAEKPDSDMSKRLMIEEITEATTSNGVIEPVTEESDQQKLLHFKRRLSSEKKEKMLDLFRVFIRQMELYNLTYMAYGGTLIGINRHHGXXXXXXXXXXXXXAAQKRRVEKVMGKLHKTHKLYVVDESLHIKWKFFNINGLYTEGYDFKYPYIDIFSMKKISXXXXXXXXXXXSNMDLHDIYPLVKRPFNGLTVYSPCNVKKILKSTYTDFNKCTSSYYSHEFEDYLRGNMIEVNCRMLKEHYPFVKKNFIAEENKIVLTERLIKNGTVLQIAKYSMPDACTNERSFYPSDLEITTFND 1243
            MIVKMKRKFLYLLLLCVSVAICLFIADLERNKYFPPLSEHLVLQEFQKFKISDCSIDQKIPSAVNVQSTVT+DYVNELI KVNDSVAEKPDSD+SKRLMIEEITEATT NGVIEPVTEESDQQKLLHFKRRLSSEKKEKMLDLFRVFIRQMELYNLTYMAYGGTLIGINRHHGIIPWDDDIDILIDAAQKRRVEKVMGKLHKTHKLYVVDESLHIKWKFFNINGLYTEGYDFKYPYIDIFSMKKISEDEVEVEGEDESNMDLHDIYPLVKRPFNGLTVYSPCNVKKILKSTYTDFNKCTSSYYSHEFEDYLRGNMIEVNCR+LKEHYPFVKKNFIAEENKIVLTERLIKNGTVLQIAKYSMPDACTNERSFYPSDLEITTFND
Sbjct:    1 MIVKMKRKFLYLLLLCVSVAICLFIADLERNKYFPPLSEHLVLQEFQKFKISDCSIDQKIPSAVNVQSTVTFDYVNELIEKVNDSVAEKPDSDLSKRLMIEEITEATTPNGVIEPVTEESDQQKLLHFKRRLSSEKKEKMLDLFRVFIRQMELYNLTYMAYGGTLIGINRHHGIIPWDDDIDILIDAAQKRRVEKVMGKLHKTHKLYVVDESLHIKWKFFNINGLYTEGYDFKYPYIDIFSMKKISEDEVEVEGEDESNMDLHDIYPLVKRPFNGLTVYSPCNVKKILKSTYTDFNKCTSSYYSHEFEDYLRGNMIEVNCRILKEHYPFVKKNFIAEENKIVLTERLIKNGTVLQIAKYSMPDACTNERSFYPSDLEITTFND 383
[Close]
BLAST of SMED30019743 vs. Planmine SMEST
Match: SMESG000023088.1 (SMESG000023088.1)

HSP 1 Score: 716.072 bits (1847), Expect = 0.000e+0
Identity = 374/379 (98.68%), Postives = 377/379 (99.47%), Query Frame = 2 
Query:  107 MKRKFLYLLLLCVSVAICLFIADLERNKYFPPLSEHLVLQEFQKFKISDCSIDQKIPSAVNVQSTVTYDYVNELIGKVNDSVAEKPDSDMSKRLMIEEITEATTSNGVIEPVTEESDQQKLLHFKRRLSSEKKEKMLDLFRVFIRQMELYNLTYMAYGGTLIGINRHHGXXXXXXXXXXXXXAAQKRRVEKVMGKLHKTHKLYVVDESLHIKWKFFNINGLYTEGYDFKYPYIDIFSMKKISXXXXXXXXXXXSNMDLHDIYPLVKRPFNGLTVYSPCNVKKILKSTYTDFNKCTSSYYSHEFEDYLRGNMIEVNCRMLKEHYPFVKKNFIAEENKIVLTERLIKNGTVLQIAKYSMPDACTNERSFYPSDLEITTFND 1243
            MKRKFLYLLLLCVSVAICLFIADLERNKYFPPLSEHLVLQEFQKFKISDCSIDQKIPSAVNVQSTVT+DYVNELI KVNDSVAEKPDSD+SKRLMIEEITEATT NGVIEPVTEESDQQKLLHFKRRLSSEKKEKMLDLFRVFIRQMELYNLTYMAYGGTLIGINRHHGIIPWDDDIDILIDAAQKRRVEKVMGKLHKTHKLYVVDESLHIKWKFFNINGLYTEGYDFKYPYIDIFSMKKISEDEVEVEGEDESNMDLHDIYPLVKRPFNGLTVYSPCNVKKILKSTYTDFNKCTSSYYSHEFEDYLRGNMIEVNCR+LKEHYPFVKKNFIAEENKIVLTERLIKNGTVLQIAKYSMPDACTNERSFYPSDLEITTFND
Sbjct:    1 MKRKFLYLLLLCVSVAICLFIADLERNKYFPPLSEHLVLQEFQKFKISDCSIDQKIPSAVNVQSTVTFDYVNELIEKVNDSVAEKPDSDLSKRLMIEEITEATTPNGVIEPVTEESDQQKLLHFKRRLSSEKKEKMLDLFRVFIRQMELYNLTYMAYGGTLIGINRHHGIIPWDDDIDILIDAAQKRRVEKVMGKLHKTHKLYVVDESLHIKWKFFNINGLYTEGYDFKYPYIDIFSMKKISEDEVEVEGEDESNMDLHDIYPLVKRPFNGLTVYSPCNVKKILKSTYTDFNKCTSSYYSHEFEDYLRGNMIEVNCRILKEHYPFVKKNFIAEENKIVLTERLIKNGTVLQIAKYSMPDACTNERSFYPSDLEITTFND 379
[Close]
BLAST of SMED30019743 vs. Planmine SMEST
Match: SMESG000052426.1 (SMESG000052426.1)

HSP 1 Score: 160.614 bits (405), Expect = 1.107e-45
Identity = 101/257 (39.30%), Postives = 149/257 (57.98%), Query Frame = 2 
Query:  473 HFKRRLSSEKKEKMLDLFRVFIRQMELYNLTYMAYGGTLIGINRHHGXXXXXXXXXXXXXAAQKRRVEKVMGKLHKTHKLYVVDESLHIKWKFFNINGLYTEGYDFKYPYIDIFSMKKISXXXXXXXXXXXSNMDLHDIYPLVKRPFNGLTVYSPCNVKKILKSTYTDFNKCTSSYYSHEFEDYLRGNMIEVNCRMLKEHYPFVKKNFIAEENKIVLTERLIKNGTVLQIAKYSMPDACTNERSFYPSDLEITTFND 1243
            +F +R+S+ +  +ML+L +VFI  ++  N+TY+AYGGTLIGI RHHG+IPWDDDIDILID  QK  ++ +   +   HKL   D      WKF+N+N     G  FKYPYIDIF  +  S   +  E   E N  +  ++PL+KR FNG+++ +PC +K +L+S Y + + C S YY+H  E Y+  +   V C  LK+ Y FV +      ++  + E LIK   ++    YS+P  C  +     SD+ +   ND
Sbjct:   45 YFYKRMSNSQTFEMLELLQVFINLLDRSNITYVAYGGTLIGIYRHHGMIPWDDDIDILIDGNQKELLKLIAKNISAPHKLLSYDNKPTGHWKFYNLNSATINGKPFKYPYIDIFFFQYSSNGTIYDESYPEFNTTILRMFPLIKRFFNGISINTPCQIKPVLESFYGNLDNCHSPYYNHSKEHYITHHR-SVKCEKLKKKYSFVVRKISENSSRKTIVETLIKRTKIIHKLVYSVPKQCNGKMKILKSDMNVFLIND 300
[Close]
BLAST of SMED30019743 vs. Planmine SMEST
Match: SMESG000060269.1 (SMESG000060269.1)

HSP 1 Score: 128.642 bits (322), Expect = 1.525e-33
Identity = 91/253 (35.97%), Postives = 142/253 (56.13%), Query Frame = 2 
Query:  473 HFKRRLSSEKKEKMLDLFRVFIRQMELYNLTYMAYGGTLIGINRHHGXXXXXXXXXXXXXAAQKRRVEKVMGKLHKTHKLYVVDESLHIKWKFFNINGLYTEGYDFKYPYIDIFSMKKISXXXXXXXXXXXSNMDLHDIYPLVKRPFNGLTVYSPCNVKKILKSTYTDFNKCTSSYYSHEFEDYL-RGNMIEVNCRMLKEHYPFVKKNFIAEENKIVLTERLIKNGTVLQIAKYSMPDACTNERSFYPSDLEI 1228
            +F   ++  +K  MLDL  +F  QM+ +N+T+  YGG+LIG  RH G IPWDDDID+++ ++ K  +   + +L++TH L   D     KWKF+ ++G       F+YPY+DIF +K+ +E   +VE E       H ++PL+ R F+ L + +PC    IL+  Y D NKCTS  ++H  E ++   N   V+C +LK  YPF +K     +N+  + E L      + IA+Y  P  C ++ S + S L I
Sbjct:   57 YFHHTMTPIQKSGMLDLLALFKSQMDKFNITFSLYGGSLIGAVRHQGFIPWDDDIDLIVWSSFKSNMISALEELNQTHNLISYDGKETGKWKFYKLDGKKFSHKPFRYPYLDIFFLKEFNETMFKVEIESYFIKKKH-MFPLILRNFDNLQMPTPCATDDILEYMYGDLNKCTSPSWNHSTEYFVSSSNWKTVSCDLLKPFYPFTEKKVHKMKNQFRIEESLKLGPKPIHIAEYYRPLRCLDKLSHFNSKLSI 308
[Close]
BLAST of SMED30019743 vs. Planmine SMEST
Match: SMESG000053934.1 (SMESG000053934.1)

HSP 1 Score: 125.561 bits (314), Expect = 4.549e-32
Identity = 115/385 (29.87%), Postives = 194/385 (50.39%), Query Frame = 2 
Query:   95 MIVKMKRKFLYLLLLCVSVAICLF-IADLERNKYFPPLSE-HLVLQEFQKFKISDCSIDQKIPSAVNVQSTVTYDYVNELIGKVNDSVAEKPDSDMSKRLM--IEEITEATTS-----NGVIEPVTEESDQQKLLHFKRRLSSEKKEKMLDLFRVFIRQMELYNLTYMAYGGTLIGINRHHGXXXXXXXXXXXXXAAQKRRVEKVMGKLHKTHKLYVVDESLHIKWKFFNINGLYTEGYDFKYPYIDIFSMKKISXXXXXXXXXXXSNMDLHD--------IYPLVKRPFNGLTVYSPCNVKKILKSTYTDFNKCTSSYYSHEFEDYLR-GNMIEVNCRMLKEHYPFVKKNFIAEENKIVLTERLIKNGTVLQIAKYSMPDACTN 1195
            MI+ ++ K+L+L+LL + V I  + +   +  +    L+E +   ++  KF+  D S                   VNE    +N+++ EK      K  M  I++I+   +S     + +   +T   +Q+    FKRRLS +  + ++ LF+  I++++   + YMA+ GTL+GI+RHHG+I WDD I ILI+   K  V KV+ + H     Y V E + IK    N   L      +++P+IDIF M K S         ++ N++++D        +YPL+KRPF+G+ V +PCN+  IL   Y +  +C S  Y+H +E  +   N++  NC +L+   PFVK++ +    ++   E L+    VL    YS P  C +
Sbjct:    1 MIISIRYKYLFLILLGLGVFIGFYYVHHKDSEQGSNKLTELYKSFKQKLKFENEDNS-------------------VNEDSKTLNENIPEKEQKREVKTTMKIIQKISTRPSSVVQKASTMSNLMTTRKEQEFYSFFKRRLSEKLLKDLIQLFKTCIKKLDKSKIPYMAFSGTLLGIHRHHGLIDWDDKISILINRDIKEDVIKVLNQTH-----YEVKE-VSIKH---NSTKLQFSSIRYRFPFIDIFFMTKQS--------NNDRNVEINDNFLIRGKYLYPLIKRPFHGIHVNTPCNISGILNDLYGNITQCQSPVYNHAYEPKITLTNVVLKNCSLLENSVPFVKRSVMKNATQLTYYEDLVLKSNVLSNFIYSSPLKCND 349
The following BLAST results are available for this feature:
BLAST of SMED30019743 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019743 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019743 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019743 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019743 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019743 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019743 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019743 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0L8FFU74.743e-2332.26Uncharacterized protein OS=Octopus bimaculoides OX... [more]
R7T3931.279e-2236.67Uncharacterized protein OS=Capitella teleta OX=283... [more]
A0A0L8GPU79.094e-2030.00Uncharacterized protein OS=Octopus bimaculoides OX... [more]
R7T6X91.700e-1935.27Uncharacterized protein OS=Capitella teleta OX=283... [more]
A0A3S1B5A03.909e-1932.30Uncharacterized protein (Fragment) OS=Elysia chlor... [more]
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BLAST of SMED30019743 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019743 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019743 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019743 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019743 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019743 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000023088.10.000e+098.69SMESG000023088.1[more]
SMESG000023088.10.000e+098.68SMESG000023088.1[more]
SMESG000052426.11.107e-4539.30SMESG000052426.1[more]
SMESG000060269.11.525e-3335.97SMESG000060269.1[more]
SMESG000053934.14.549e-3229.87SMESG000053934.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30019743 ID=SMED30019743|Name=SMED30019743|organism=Schmidtea mediterranea sexual|type=transcript|length=1328bp
CTTGAGTTCTACCTAAACTGCGTTGGCAGAATCATGACAGGTCTTGTAAT
TATACACGTGGCAGTCAACTGATTTCAATATACCAATATAATACATGATA
GTAAAAATGAAGCGAAAGTTTCTTTATTTATTATTGTTATGTGTATCCGT
GGCAATTTGTCTTTTTATTGCTGATTTGGAAAGAAATAAATATTTCCCAC
CTCTTTCTGAACATTTGGTGCTTCAAGAGTTCCAGAAATTTAAGATAAGT
GACTGTTCAATTGATCAAAAGATTCCATCGGCTGTAAATGTGCAATCCAC
TGTGACATACGATTATGTAAATGAACTGATTGGAAAGGTCAATGATTCGG
TTGCAGAGAAGCCTGACAGTGATATGTCAAAAAGACTTATGATAGAGGAA
ATAACTGAAGCGACAACCTCAAATGGCGTCATTGAACCCGTCACTGAAGA
ATCAGACCAACAAAAGTTGCTGCATTTTAAAAGAAGATTGTCAAGTGAAA
AAAAAGAAAAAATGTTGGATTTATTTCGAGTTTTTATTCGGCAAATGGAA
TTGTACAATTTGACCTATATGGCTTATGGTGGAACATTGATAGGAATCAA
CCGACATCACGGCATTATTCCTTGGGACGATGATATAGACATTTTAATAG
ATGCAGCACAGAAACGACGGGTTGAAAAGGTCATGGGTAAACTGCATAAA
ACTCATAAATTATATGTAGTAGATGAAAGTCTTCACATTAAATGGAAGTT
CTTCAACATAAACGGCTTATACACCGAAGGATACGATTTCAAATACCCTT
ATATTGATATATTTTCTATGAAGAAAATTTCTGAAGACGAGGTTGAGGTT
GAAGGAGAGGATGAATCAAATATGGATCTCCATGACATTTATCCGTTAGT
CAAACGACCTTTTAACGGATTGACCGTTTATTCGCCATGTAATGTAAAAA
AAATTTTAAAAAGTACTTACACTGATTTCAATAAATGCACTAGTTCTTAT
TATAGTCATGAGTTTGAGGATTATCTTCGGGGAAACATGATAGAAGTTAA
TTGTAGAATGTTGAAAGAGCATTATCCTTTTGTCAAAAAAAACTTCATTG
CGGAAGAAAATAAAATTGTTTTAACTGAAAGACTGATAAAAAATGGGACT
GTTTTACAAATTGCGAAGTATTCGATGCCTGATGCGTGCACAAATGAAAG
GTCTTTTTACCCTTCCGATTTAGAGATTACGACATTTAATGATTGATGTA
TTTTTATGGAGTAAACTTTATTAAATTTCTCATTTATTGTATTTTTAAAT
ATACATTTCTTTATTTTGTTTAAAAAAA
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protein sequence of SMED30019743-orf-1

>SMED30019743-orf-1 ID=SMED30019743-orf-1|Name=SMED30019743-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=384bp
MIVKMKRKFLYLLLLCVSVAICLFIADLERNKYFPPLSEHLVLQEFQKFK
ISDCSIDQKIPSAVNVQSTVTYDYVNELIGKVNDSVAEKPDSDMSKRLMI
EEITEATTSNGVIEPVTEESDQQKLLHFKRRLSSEKKEKMLDLFRVFIRQ
MELYNLTYMAYGGTLIGINRHHGIIPWDDDIDILIDAAQKRRVEKVMGKL
HKTHKLYVVDESLHIKWKFFNINGLYTEGYDFKYPYIDIFSMKKISEDEV
EVEGEDESNMDLHDIYPLVKRPFNGLTVYSPCNVKKILKSTYTDFNKCTS
SYYSHEFEDYLRGNMIEVNCRMLKEHYPFVKKNFIAEENKIVLTERLIKN
GTVLQIAKYSMPDACTNERSFYPSDLEITTFND*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0002032epidermal cell
PLANA:0002111X2 cell
PLANA:0002142parenchyma
Vocabulary: INTERPRO
TermDefinition
IPR007074LicD_fam
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
Vocabulary: molecular function
TermDefinition
GO:0016740transferase activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007074LicD familyPFAMPF04991LicDcoord: 154..251
e-value: 2.9E-13
score: 50.5
NoneNo IPR availablePANTHERPTHR43404FAMILY NOT NAMEDcoord: 98..319
NoneNo IPR availableTMHMMTMhelixcoord: 9..28