inositol phosphoceramide mannosyltransferase 2-like

Overview
Nameinositol phosphoceramide mannosyltransferase 2-like
Smed IDSMED30019717
Length (bp)1126
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of inositol phosphoceramide mannosyltransferase 2-like (SMED30019717) t-SNE clustered cells

Violin plots show distribution of expression levels for inositol phosphoceramide mannosyltransferase 2-like (SMED30019717) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of inositol phosphoceramide mannosyltransferase 2-like (SMED30019717) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for inositol phosphoceramide mannosyltransferase 2-like (SMED30019717) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30019717

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30019717h1SMcG0001689 OX_Smed_1.0.16424ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of inositol phosphoceramide mannosyltransferase 2-like vs. UniProt/SwissProt
Match: sp|Q10323|IMT3_SCHPO (Inositol phosphoceramide mannosyltransferase 3 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC17G8.11c PE=1 SV=1)

HSP 1 Score: 97.8265 bits (242), Expect = 1.578e-21
Identity = 49/108 (45.37%), Postives = 68/108 (62.96%), Query Frame = 3 
Query:  324 QLNNCENENVTKIIHVSWKTNDIPVVFEKYLKSWTDFCYKDWKFMFWTDEDNYNLIKTFYPWFLKKYNEFPRNIYRADVSRYFYLFHYGGIYTDLDNECLRPFEHLLQ 647
            ++ + ++  + KIIH +WKTN+IP  +    KS  D  + D++++ WTDE     I T YPWFL +Y+ +P NI RADV RYF L+ YGGIY DLD  C R  + LL 
Sbjct:   48 EIYSNQSAAIPKIIHQTWKTNEIPEKWVGAQKSCIDL-HPDYEYVLWTDESMREFIATDYPWFLTQYDSYPYNIERADVVRYFILYKYGGIYLDLDVGCNRTLDPLLH 154
[Close]
BLAST of inositol phosphoceramide mannosyltransferase 2-like vs. UniProt/SwissProt
Match: sp|P33300|SUR1_YEAST (Mannosyl phosphorylinositol ceramide synthase SUR1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SUR1 PE=1 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 4.838e-21
Identity = 68/200 (34.00%), Postives = 101/200 (50.50%), Query Frame = 3 
Query:  351 VTKIIHVSWKTNDIPVVFEKYLKSWTDFCYKDWKFMFWTDEDNYNLIKTFYPWFLKKYNEFPRNIYRADVSRYFYLFHYGGIYTDLDNECLRPFEHLLQNYSIVFGAMKGQYLGTNLKEGYVQNSFMYSRPRQTFWMELLN-----DILMFKGTNSVEVQTGPVLLSKRIVLFRE----RLNSSDYMMIYSPNYFNPFSW 923
            + KIIH ++KT DIP  +++  +   D  + D+K++ WTDE  Y  IK  YPWFL  +  +   I RAD  RYF L HYGG+Y DLD+ C R  + LL   + +    K   LG       V N  M S PR  F+++ L      D   F    ++   TGP+ LS   V++++    R+  +  + I  P Y+   S+
Sbjct:   55 IPKIIHQTYKTEDIPEHWKEGRQKCLDL-HPDYKYILWTDEMAYEFIKEEYPWFLDTFENYKYPIERADAIRYFILSHYGGVYIDLDDGCERKLDPLLAFPAFL---RKTSPLG-------VSNDVMGSVPRHPFFLKALKSLKHYDKYWFIPYMTIMGSTGPLFLS---VIWKQYKRWRIPKNGTVRILQPAYYKMHSY 240
[Close]
BLAST of inositol phosphoceramide mannosyltransferase 2-like vs. UniProt/SwissProt
Match: sp|P38287|CSH1_YEAST (Mannosyl phosphorylinositol ceramide synthase CSH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=CSH1 PE=1 SV=1)

HSP 1 Score: 94.3597 bits (233), Expect = 3.361e-20
Identity = 59/167 (35.33%), Postives = 86/167 (51.50%), Query Frame = 3 
Query:  351 VTKIIHVSWKTNDIPVVFEKYLKSWTDFCYKDWKFMFWTDEDNYNLIKTFYPWFLKKYNEFPRNIYRADVSRYFYLFHYGGIYTDLDNECLRPFEHLLQNYSIVFGAMKGQYLGTNLKEGYVQNSFMYSRPRQTFWMELLNDILMFKGT-----NSVEVQTGPVLLS 836
            + KIIH ++KTNDIP  + K  +   D  + D+ ++ WTDE +   IK  YPWFL  +  +   I RAD  RYF L HYGGIY DLD+ C R  + LL+            +L      G V N  M S PR  F+++++  +  +K        ++   TGP+ +S
Sbjct:   62 IPKIIHQTYKTNDIPEQWVKGRQKCIDL-HPDYTYILWTDEMSDTFIKQEYPWFLDTFRSYEYPIERADAIRYFILSHYGGIYIDLDDGCERRLDPLLK---------VPAFLRKTSPTG-VSNDVMGSVPRHPFFLKVIKSLKHYKKNWYIPYMTIMGSTGPLFIS 217
[Close]
BLAST of inositol phosphoceramide mannosyltransferase 2-like vs. UniProt/SwissProt
Match: sp|Q9UT67|IMT2_SCHPO (Inositol phosphoceramide mannosyltransferase 2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPCC4F11.04c PE=3 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 8.833e-20
Identity = 47/108 (43.52%), Postives = 68/108 (62.96%), Query Frame = 3 
Query:  324 QLNNCENENV-TKIIHVSWKTNDIPVVFEKYLKSWTDFCYKDWKFMFWTDEDNYNLIKTFYPWFLKKYNEFPRNIYRADVSRYFYLFHYGGIYTDLDNECLRPFEHLL 644
             LN+   E V  KIIH +WKT ++P  ++   +S  D  + D++++ WTDE + N I   YPWFL  ++ +P N+ RADV RYF L+HYGG Y DLD+ C +  + LL
Sbjct:   72 SLNDISGEPVIPKIIHQTWKTTEVPEGWKGAQQSCIDL-HPDYEYILWTDEMSRNFIADNYPWFLPYFDAYPFNVQRADVIRYFVLYHYGGNYIDLDDGCRQRLDSLL 178
[Close]
BLAST of inositol phosphoceramide mannosyltransferase 2-like vs. UniProt/SwissProt
Match: sp|O14084|IMT1_SCHPO (Inositol phosphoceramide mannosyltransferase 1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=imt1 PE=3 SV=2)

HSP 1 Score: 84.7297 bits (208), Expect = 4.485e-17
Identity = 51/177 (28.81%), Postives = 86/177 (48.59%), Query Frame = 3 
Query:  339 ENENVTKIIHVSWKTNDIPVVFEKYLKSWTDFCYKD------WKFMFWTDEDNYNLIKTFYPWFLKKYNEFPRNIYRADVSRYFYLFHYGGIYTDLDNECLRPFEHLLQNYSIVFGAMKGQYLGTNLKEGYVQNSFMYSRPRQTFWMELLNDILMFKGT-----NSVEVQTGPVLLS 836
            E E + ++IH  WK  +IP  +   + S    C +       W+F+ WTDE   + +   Y WF+  ++ +P NI + D +RYF L+HYGG+Y DLD  C +P + LL   + +        L +    GY  N +  + P+  F  + ++++  F         +V +  GP+ LS
Sbjct:   60 EMEKIPRVIHQLWKDENIPERWSNTVNS----CRRQHPDENGWQFILWTDEKIMSFMNENYSWFMPVFHSYPYNIQKFDAARYFILYHYGGVYMDLDIGCKKPMDPLLSKATFI--------LPSTEPIGY-SNDWFAATPKHPFLYQAIHNLSKFNHRYFTKYPTVFLSAGPLFLS 223
[Close]
BLAST of inositol phosphoceramide mannosyltransferase 2-like vs. TrEMBL
Match: A0A1I8H5Y8 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 189.504 bits (480), Expect = 1.261e-53
Identity = 97/249 (38.96%), Postives = 149/249 (59.84%), Query Frame = 3 
Query:  351 VTKIIHVSWKTNDIPVVFEKYLKSWTDFCYKDWKFMFWTDEDNYNLIKTFYPWFLKKYNEFPRNIYRADVSRYFYLFHYGGIYTDLDNECLRPFEHLLQNYSIVFGAMKGQYLGTN-----LKEGYVQNSFMYSRPRQTFWMELLNDILMFKGTNSVEVQTGPVLLSKRIVLFRE--------RLNSSDYMMIYSPNYFNPFSWIDKSKVDPNCKSHNQM-TRENLNKCRSSMISKGSYVVQYHAHSWG 1055
            + +I+H SW +  +P  F ++ +S++  C+ D++   WTD+DN  LI   YPWFL++Y+   R + RADVSR FY++HYGG+Y DLD ECLR F HLL N S+VFGAM+G  + +      + EG ++NSFMYSRPR  F+ +L+  +    G   + + +GP L+   I   R+        R++  D++ +Y P YFNP+SW+  +    +C+ + +   R  L +CR S   + SYV+QYH   W 
Sbjct:   62 LVRIVHQSWASRKLPEQFAEWSRSFSA-CFPDFRRRLWTDKDNRQLIARHYPWFLRRYDSLARGVQRADVSRLFYMYHYGGLYCDLDCECLRDFRHLLANASLVFGAMQGLMIRSPRDPAYVAEGQIENSFMYSRPRHPFFWDLIVYLNATTGEMPI-MSSGPYLIMSGIHAARQLGIAPSGIRVDGRDFLTVYPPAYFNPYSWMLLNSR--HCRLYGRFENRAQLRQCRDSF--QDSYVMQYHTQVWA 304
[Close]
BLAST of inositol phosphoceramide mannosyltransferase 2-like vs. TrEMBL
Match: A0A1I8HSW6 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 189.889 bits (481), Expect = 5.071e-53
Identity = 96/248 (38.71%), Postives = 143/248 (57.66%), Query Frame = 3 
Query:  351 VTKIIHVSWKTNDIPVVFEKYLKSWTDFCYKDWKFMFWTDEDNYNLIKTFYPWFLKKYNEFPRNIYRADVSRYFYLFHYGGIYTDLDNECLRPFEHLLQNYSIVFGAMKGQYLGTN-----LKEGYVQNSFMYSRPRQTFWMELLNDILMFKGTNSVEVQTGPVLL------SKRIVLFRERLNSSDYMMIYSPNYFNPFSWID--KSKVDPNCKSHNQMTRENLNKCRSS-MISKGSYVVQYHAHSW 1052
            V  I+H SWKT  +P +F     ++ D C   ++ + WTD DN +L+   YPWFL +Y+     + RADV+R FY+  YGG+Y DLD +CLR F+HLL N S+  GAM+G  +        + EG V+NSFMYS+PR  F+ EL+  +   +G   + + +G  LL      ++RI L R   +   Y+ +Y P+ FNPFSWI+  + +  P+C+   Q T ++   CR + + S  SY+VQYH   W
Sbjct:  124 VVAILHQSWKTAHLPPLFANLSATFAD-CLPSFRRILWTDSDNRDLVARHYPWFLARYDSLAPGVQRADVARLFYMHRYGGVYCDLDCDCLRGFQHLLANTSLALGAMEGSLIPERENPAFVDEGQVENSFMYSQPRHPFFWELILRLNATQGDLPI-MASGTYLLMTGIRSARRIGLDRTLFSGLPYLTVYPPSLFNPFSWINPQEGRASPDCQLFGQFTPQSRQLCRQAYLKSNASYIVQYHTQVW 369
[Close]
BLAST of inositol phosphoceramide mannosyltransferase 2-like vs. TrEMBL
Match: A0A267F5S5 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig014144g2 PE=4 SV=1)

HSP 1 Score: 187.578 bits (475), Expect = 6.460e-53
Identity = 97/249 (38.96%), Postives = 148/249 (59.44%), Query Frame = 3 
Query:  351 VTKIIHVSWKTNDIPVVFEKYLKSWTDFCYKDWKFMFWTDEDNYNLIKTFYPWFLKKYNEFPRNIYRADVSRYFYLFHYGGIYTDLDNECLRPFEHLLQNYSIVFGAMKGQYLGTN-----LKEGYVQNSFMYSRPRQTFWMELLNDILMFKGTNSVEVQTGPVLLSKRIVLFRE--------RLNSSDYMMIYSPNYFNPFSWIDKSKVDPNCKSHNQM-TRENLNKCRSSMISKGSYVVQYHAHSWG 1055
            + +I+H SW +  +P  F ++ +S++  C+ D++   WTDEDN  LI   YPWFL++Y+     + RADVSR FY++HYGG+Y DLD ECLR F HLL N S+VFGAM+G  + +      + EG ++NSFMYSRPR  F+ +L+  +    G   + + +GP L+   I   R+        R++  D++ +Y P YFNP+SW+  +    +C+ + +   R  L +CR S   + SYV+QYH   W 
Sbjct:   62 LVRIVHQSWASRKLPEQFAEWSRSFSA-CFPDFRRRLWTDEDNRELIARHYPWFLRRYDSLAPGVQRADVSRLFYMYHYGGLYCDLDCECLRDFRHLLDNASLVFGAMQGLMIRSPRDPAYVAEGQIENSFMYSRPRHPFFWDLIVYLNATTGEMPI-MSSGPYLIMSGIHAARQLGIAPSGIRVDGRDFLTVYPPAYFNPYSWMLLNSR--HCRLYGRFENRTQLRQCRDSF--QDSYVMQYHTQVWA 304
[Close]
BLAST of inositol phosphoceramide mannosyltransferase 2-like vs. TrEMBL
Match: A0A267F5L2 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig014144g3 PE=4 SV=1)

HSP 1 Score: 186.422 bits (472), Expect = 1.601e-52
Identity = 96/249 (38.55%), Postives = 148/249 (59.44%), Query Frame = 3 
Query:  351 VTKIIHVSWKTNDIPVVFEKYLKSWTDFCYKDWKFMFWTDEDNYNLIKTFYPWFLKKYNEFPRNIYRADVSRYFYLFHYGGIYTDLDNECLRPFEHLLQNYSIVFGAMKGQYLGTN-----LKEGYVQNSFMYSRPRQTFWMELLNDILMFKGTNSVEVQTGPVLLSKRIVLFRE--------RLNSSDYMMIYSPNYFNPFSWIDKSKVDPNCKSHNQM-TRENLNKCRSSMISKGSYVVQYHAHSWG 1055
            + +I+H SW +  +P  F ++ +S++  C+ D++   WTD+DN  LI   YPWFL++Y+     + RADVSR FY++HYGG+Y DLD ECLR F HLL N S+VFGAM+G  + +      + EG ++NSFMYSRPR  F+ +L+  +    G   + + +GP L+   I   R+        R++  D++ +Y P YFNP+SW+  +    +C+ + +   R  L +CR S   + SYV+QYH   W 
Sbjct:   62 LVRIVHQSWASRKLPEQFAEWSRSFSA-CFPDFRRRLWTDKDNRQLIARHYPWFLRRYDSLAPGVQRADVSRLFYMYHYGGLYCDLDCECLRDFRHLLANASLVFGAMQGLMIRSPRDPAYVAEGQIENSFMYSRPRHPFFWDLIVYLNATTGEMPI-MSSGPYLIMSGIHAARQLGIAPSGIRVDGRDFLTVYPPAYFNPYSWMLLNSR--HCRLYGRFENRAQLRQCRDSF--QDSYVMQYHTQVWA 304
[Close]
BLAST of inositol phosphoceramide mannosyltransferase 2-like vs. TrEMBL
Match: A0A1I8FXP5 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 187.193 bits (474), Expect = 1.798e-52
Identity = 96/252 (38.10%), Postives = 149/252 (59.13%), Query Frame = 3 
Query:  333 NCENENVTKIIHVSWKTNDIPVVFEKYLKSWTDFCYKDWKFMFWTDEDNYNLIKTFYPWFLKKYNEFPRNIYRADVSRYFYLFHYGGIYTDLDNECLRPFEHLLQNYSIVFGAMKGQYL-----GTNLKEGYVQNSFMYSRPRQTFWMELLNDILMFKGTNSVEVQT-GPVLLSKRIVLFRER------LNSSDYMMIYSPNYFNPFSWIDKSKVDPNCKSHNQMTRENLNKCRSSMISKGSYVVQYHAHSW 1052
               ++ V +++H SW    +P +F ++  S+   C+  +++  WTDEDN  LI   YPWFL +++ +   + +ADVSR FY++HYGG+Y DLD ECL  FEH+L N S+ FGAM G+ +      + + EG ++NSFMYSRP+  F+ EL+   ++   T  + + T GP L+ + I   R+R       +   Y+ IY P+ FNP+SWI  +   P+C+     + E+L +CR+S   K SYV+QYH   W
Sbjct:   82 GSRDQPVIRLVHQSWVNATLPPLFARWAGSFGR-CFPTYRYALWTDEDNRRLIAEHYPWFLARFDSYGVGVQKADVSRLFYMYHYGGLYCDLDCECLANFEHMLANTSLAFGAMDGKMIYSSSDSSYVDEGQIENSFMYSRPKHPFFWELIR--VLNTTTEGLPIMTSGPYLIMRGIHSARKRGLDKTKFDGLPYLTIYPPHLFNPYSWIRHNA--PSCRLFGDFSEESLARCRNSF--KTSYVIQYHTQVW 326
[Close]
BLAST of inositol phosphoceramide mannosyltransferase 2-like vs. Ensembl Yeast
Match: SUR1 (Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Csh1p; SUR1 has a paralog, CSH1, that arose from the whole genome duplication [Source:SGD;Acc:S000005978])

HSP 1 Score: 97.0561 bits (240), Expect = 6.968e-23
Identity = 68/200 (34.00%), Postives = 101/200 (50.50%), Query Frame = 3 
Query:  351 VTKIIHVSWKTNDIPVVFEKYLKSWTDFCYKDWKFMFWTDEDNYNLIKTFYPWFLKKYNEFPRNIYRADVSRYFYLFHYGGIYTDLDNECLRPFEHLLQNYSIVFGAMKGQYLGTNLKEGYVQNSFMYSRPRQTFWMELLN-----DILMFKGTNSVEVQTGPVLLSKRIVLFRE----RLNSSDYMMIYSPNYFNPFSW 923
            + KIIH ++KT DIP  +++  +   D  + D+K++ WTDE  Y  IK  YPWFL  +  +   I RAD  RYF L HYGG+Y DLD+ C R  + LL   + +    K   LG       V N  M S PR  F+++ L      D   F    ++   TGP+ LS   V++++    R+  +  + I  P Y+   S+
Sbjct:   55 IPKIIHQTYKTEDIPEHWKEGRQKCLDL-HPDYKYILWTDEMAYEFIKEEYPWFLDTFENYKYPIERADAIRYFILSHYGGVYIDLDDGCERKLDPLLAFPAFL---RKTSPLG-------VSNDVMGSVPRHPFFLKALKSLKHYDKYWFIPYMTIMGSTGPLFLS---VIWKQYKRWRIPKNGTVRILQPAYYKMHSY 240
[Close]
BLAST of inositol phosphoceramide mannosyltransferase 2-like vs. Ensembl Yeast
Match: CSH1 (Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Sur1p; CSH1 has a paralog, SUR1, that arose from the whole genome duplication [Source:SGD;Acc:S000000365])

HSP 1 Score: 94.3597 bits (233), Expect = 4.840e-22
Identity = 59/167 (35.33%), Postives = 86/167 (51.50%), Query Frame = 3 
Query:  351 VTKIIHVSWKTNDIPVVFEKYLKSWTDFCYKDWKFMFWTDEDNYNLIKTFYPWFLKKYNEFPRNIYRADVSRYFYLFHYGGIYTDLDNECLRPFEHLLQNYSIVFGAMKGQYLGTNLKEGYVQNSFMYSRPRQTFWMELLNDILMFKGT-----NSVEVQTGPVLLS 836
            + KIIH ++KTNDIP  + K  +   D  + D+ ++ WTDE +   IK  YPWFL  +  +   I RAD  RYF L HYGGIY DLD+ C R  + LL+            +L      G V N  M S PR  F+++++  +  +K        ++   TGP+ +S
Sbjct:   62 IPKIIHQTYKTNDIPEQWVKGRQKCIDL-HPDYTYILWTDEMSDTFIKQEYPWFLDTFRSYEYPIERADAIRYFILSHYGGIYIDLDDGCERRLDPLLK---------VPAFLRKTSPTG-VSNDVMGSVPRHPFFLKVIKSLKHYKKNWYIPYMTIMGSTGPLFIS 217
[Close]
BLAST of inositol phosphoceramide mannosyltransferase 2-like vs. Ensembl Yeast
Match: OCH1 (Mannosyltransferase of the cis-Golgi apparatus; initiates the polymannose outer chain elongation of N-linked oligosaccharides of glycoproteins [Source:SGD;Acc:S000003006])

HSP 1 Score: 55.0694 bits (131), Expect = 9.027e-9
Identity = 26/84 (30.95%), Postives = 43/84 (51.19%), Query Frame = 3 
Query:  393 PVVFEKYLKSWTDFCYKDWKFMFWTDEDNYNLIKTFY---PWFLKKYNEFPRNIYRADVSRYFYLFHYGGIYTDLDNECLRPFE 635
            P  F  Y K+W+     D+++   +D+     ++  Y   P  ++ +   P NI +AD  RY  LF  GGIY+D+D   L+P +
Sbjct:  114 PSSFRTYQKTWSGSYSPDYQYSLISDDSIIPFLENLYAPVPIVIQAFKLMPGNILKADFLRYLLLFARGGIYSDMDTMLLKPID 197
[Close]
BLAST of inositol phosphoceramide mannosyltransferase 2-like vs. Planmine SMEST
Match: SMESG000045480.1 (SMESG000045480.1)

HSP 1 Score: 537.339 bits (1383), Expect = 0.000e+0
Identity = 259/284 (91.20%), Postives = 267/284 (94.01%), Query Frame = 3 
Query:  213 MSKFMKIFFL---LTSAIFIGIFFVENYERFYIVKQSIQIQLNNCENENVTKIIHVSWKTNDIPVVFEKYLKSWTDFCYKDWKFMFWTDEDNYNLIKTFYPWFLKKYNEFPRNIYRADVSRYFYLFHYGGIYTDLDNECLRPFEHLLQNYSIVFGAMKGQYLGTNLKEGYVQNSFMYSRPRQTFWMELLNDILMFKGTNSVEVQTGPVLLSKRIVLFRERLNSSDYMMIYSPNYFNPFSWIDKSKVDPNCKSHNQMTRENLNKCRSSMISKGSYVVQYHAHSWG 1055
            MSK  KIFFL   L +AIFIGIFF+ENYERFYIV+Q IQ  L NCE E VTKIIHVSWKTNDIPVVF+KYLKSWTDFCYKDWKFMFWTD+DNYNLIKTFYPWFLK YNEFPRNIYRADVSRYFYLFHYGGIYTDLDNECLRPFEHLLQNYSIVFGAMKGQYLGTNLKEGYVQNSFMYSRPRQTFWMELLNDILMFKGT+SVEVQTGPVLLSKRI L+RERLNSSD MMIYSPNYFNPFSWID SKVDPNCKS NQMTRENLNKCRSSM+SKGSYVVQYHAHSWG
Sbjct:    1 MSKVKKIFFLFLALGNAIFIGIFFIENYERFYIVEQPIQTLLKNCEKEKVTKIIHVSWKTNDIPVVFQKYLKSWTDFCYKDWKFMFWTDKDNYNLIKTFYPWFLKTYNEFPRNIYRADVSRYFYLFHYGGIYTDLDNECLRPFEHLLQNYSIVFGAMKGQYLGTNLKEGYVQNSFMYSRPRQTFWMELLNDILMFKGTDSVEVQTGPVLLSKRIALYRERLNSSDDMMIYSPNYFNPFSWIDNSKVDPNCKSPNQMTRENLNKCRSSMLSKGSYVVQYHAHSWG 284
[Close]
BLAST of inositol phosphoceramide mannosyltransferase 2-like vs. Planmine SMEST
Match: SMESG000045292.1 (SMESG000045292.1)

HSP 1 Score: 524.242 bits (1349), Expect = 0.000e+0
Identity = 252/284 (88.73%), Postives = 263/284 (92.61%), Query Frame = 3 
Query:  213 MSKFMKIFFL---LTSAIFIGIFFVENYERFYIVKQSIQIQLNNCENENVTKIIHVSWKTNDIPVVFEKYLKSWTDFCYKDWKFMFWTDEDNYNLIKTFYPWFLKKYNEFPRNIYRADVSRYFYLFHYGGIYTDLDNECLRPFEHLLQNYSIVFGAMKGQYLGTNLKEGYVQNSFMYSRPRQTFWMELLNDILMFKGTNSVEVQTGPVLLSKRIVLFRERLNSSDYMMIYSPNYFNPFSWIDKSKVDPNCKSHNQMTRENLNKCRSSMISKGSYVVQYHAHSWG 1055
            MS+  KIFFL   L +AIFIG FF++NYERFYIV+Q IQ  L NCE E VTKIIHVSWKTNDIPVVF+KYLKSWTDFCYKDWKFMFWTD+DNYNLIKTFYPWFLK YNEFP NI+RADVSRYFYLFHYGGIYTDLDNECLRPFEHLLQNYS+VFGAMKGQYLGTNLKEGYVQNSFMYSRPRQTFWMELLNDILM KGT SVEVQTGPVLLSKRI L+RERLNSSD MMIYSPNYFNPFSWID SKVDPNCKSHN+MT ENLNKCRSSMISKGSYVVQYHAHSWG
Sbjct:    1 MSRATKIFFLVLALGNAIFIGTFFIKNYERFYIVEQPIQTLLKNCEKEKVTKIIHVSWKTNDIPVVFQKYLKSWTDFCYKDWKFMFWTDKDNYNLIKTFYPWFLKTYNEFPWNIFRADVSRYFYLFHYGGIYTDLDNECLRPFEHLLQNYSLVFGAMKGQYLGTNLKEGYVQNSFMYSRPRQTFWMELLNDILMSKGTGSVEVQTGPVLLSKRIALYRERLNSSDDMMIYSPNYFNPFSWIDNSKVDPNCKSHNKMTLENLNKCRSSMISKGSYVVQYHAHSWG 284
[Close]
BLAST of inositol phosphoceramide mannosyltransferase 2-like vs. Planmine SMEST
Match: SMESG000045267.1 (SMESG000045267.1)

HSP 1 Score: 516.538 bits (1329), Expect = 0.000e+0
Identity = 258/284 (90.85%), Postives = 265/284 (93.31%), Query Frame = 3 
Query:  213 MSKFMKIFFL---LTSAIFIGIFFVENYERFYIVKQSIQIQLNNCENENVTKIIHVSWKTNDIPVVFEKYLKSWTDFCYKDWKFMFWTDEDNYNLIKTFYPWFLKKYNEFPRNIYRADVSRYFYLFHYGGIYTDLDNECLRPFEHLLQNYSIVFGAMKGQYLGTNLKEGYVQNSFMYSRPRQTFWMELLNDILMFKGTNSVEVQTGPVLLSKRIVLFRERLNSSDYMMIYSPNYFNPFSWIDKSKVDPNCKSHNQMTRENLNKCRSSMISKGSYVVQYHAHSWG 1055
            MSKF KIFFL   L   IFIGIFF+ENYERFYIV+Q IQ  L NCE E VTKIIHVSWKTNDIPVVF+KYLKSWTDFCYKDW FMFWTD+DNYNLIKTFYPWFLK YN+FPRNIYRADVSRYFYLFHYGGIYTDLDNECLRPFEHLLQNYSIVFGAMKGQYLGTNLKEGYVQNSFMYSRPRQTFWMELLNDILMFKGT+SVEVQTGPVLLSKRI L+RERLNSSD MMIYSPNYFNPFSWID SKVD NCKSHNQMTRENLNKCRSSMISKGSYVVQYHAHSWG
Sbjct:    1 MSKFKKIFFLVLALGIVIFIGIFFIENYERFYIVEQPIQTLLKNCEKEKVTKIIHVSWKTNDIPVVFQKYLKSWTDFCYKDWNFMFWTDKDNYNLIKTFYPWFLKTYNKFPRNIYRADVSRYFYLFHYGGIYTDLDNECLRPFEHLLQNYSIVFGAMKGQYLGTNLKEGYVQNSFMYSRPRQTFWMELLNDILMFKGTDSVEVQTGPVLLSKRIALYRERLNSSDDMMIYSPNYFNPFSWIDNSKVDLNCKSHNQMTRENLNKCRSSMISKGSYVVQYHAHSWG 284
[Close]
BLAST of inositol phosphoceramide mannosyltransferase 2-like vs. Planmine SMEST
Match: SMESG000037990.1 (SMESG000037990.1)

HSP 1 Score: 504.597 bits (1298), Expect = 0.000e+0
Identity = 242/273 (88.64%), Postives = 251/273 (91.94%), Query Frame = 3 
Query:  243 LTSAIFIGIFFVENYERFYIVKQSIQIQLNNCENENVTKIIHVSWKTNDIPVVFEKYLKSWTDFCYKDWKFMFWTDEDNYNLIKTFYPWFLKKYNEFPRNIYRADVSRYFYLFHYGGIYTDLDNECLRPFEHLLQNYSIVFGAMKGQYLGTNLKEGYVQNSFMYSRPRQTFWMELLNDILMFKGTNSVEVQTGPVLLSKR--IVLFRERLNSSDYMMIYSPNYFNPFSWIDKSKVDPNCKSHNQMTRENLNKCRSSMISKGSYVVQYHAHSWG 1055
            L +AIFIG FF+ENYERFYIV+Q IQ  L NCENE VTKIIHVSWKTNDIPV F+KYLKSWTDFCYKDWKFMFWTD+DNYNLIKTFYPWFLK YN+FPRNIYR DVSRYFYLFHYGGIYTDLDNECLRPFEHLLQNYSIVFGAMKGQYLGTNLKEGYVQNSFMYSRPRQTFWMELLNDILMF GT+SVEVQTGPVLLSK   I L+RERLNSSD MMIYSPNYFNP SWI KSKVDPNCK HNQMT ENL KC+SSMIS GSYVVQYHAHSWG
Sbjct:   14 LGNAIFIGTFFIENYERFYIVEQPIQTLLKNCENEKVTKIIHVSWKTNDIPVEFQKYLKSWTDFCYKDWKFMFWTDKDNYNLIKTFYPWFLKTYNKFPRNIYRVDVSRYFYLFHYGGIYTDLDNECLRPFEHLLQNYSIVFGAMKGQYLGTNLKEGYVQNSFMYSRPRQTFWMELLNDILMFNGTDSVEVQTGPVLLSKSFIIALYRERLNSSDDMMIYSPNYFNPLSWIIKSKVDPNCKFHNQMTLENLKKCKSSMISTGSYVVQYHAHSWG 286
[Close]
BLAST of inositol phosphoceramide mannosyltransferase 2-like vs. Planmine SMEST
Match: SMESG000059812.1 (SMESG000059812.1)

HSP 1 Score: 420.239 bits (1079), Expect = 2.759e-149
Identity = 200/200 (100.00%), Postives = 200/200 (100.00%), Query Frame = 3 
Query:  456 MFWTDEDNYNLIKTFYPWFLKKYNEFPRNIYRADVSRYFYLFHYGGIYTDLDNECLRPFEHLLQNYSIVFGAMKGQYLGTNLKEGYVQNSFMYSRPRQTFWMELLNDILMFKGTNSVEVQTGPVLLSKRIVLFRERLNSSDYMMIYSPNYFNPFSWIDKSKVDPNCKSHNQMTRENLNKCRSSMISKGSYVVQYHAHSWG 1055
            MFWTDEDNYNLIKTFYPWFLKKYNEFPRNIYRADVSRYFYLFHYGGIYTDLDNECLRPFEHLLQNYSIVFGAMKGQYLGTNLKEGYVQNSFMYSRPRQTFWMELLNDILMFKGTNSVEVQTGPVLLSKRIVLFRERLNSSDYMMIYSPNYFNPFSWIDKSKVDPNCKSHNQMTRENLNKCRSSMISKGSYVVQYHAHSWG
Sbjct:    1 MFWTDEDNYNLIKTFYPWFLKKYNEFPRNIYRADVSRYFYLFHYGGIYTDLDNECLRPFEHLLQNYSIVFGAMKGQYLGTNLKEGYVQNSFMYSRPRQTFWMELLNDILMFKGTNSVEVQTGPVLLSKRIVLFRERLNSSDYMMIYSPNYFNPFSWIDKSKVDPNCKSHNQMTRENLNKCRSSMISKGSYVVQYHAHSWG 200
The following BLAST results are available for this feature:
BLAST of inositol phosphoceramide mannosyltransferase 2-like vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of inositol phosphoceramide mannosyltransferase 2-like vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of inositol phosphoceramide mannosyltransferase 2-like vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of inositol phosphoceramide mannosyltransferase 2-like vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of inositol phosphoceramide mannosyltransferase 2-like vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of inositol phosphoceramide mannosyltransferase 2-like vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of inositol phosphoceramide mannosyltransferase 2-like vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q10323|IMT3_SCHPO1.578e-2145.37Inositol phosphoceramide mannosyltransferase 3 OS=... [more]
sp|P33300|SUR1_YEAST4.838e-2134.00Mannosyl phosphorylinositol ceramide synthase SUR1... [more]
sp|P38287|CSH1_YEAST3.361e-2035.33Mannosyl phosphorylinositol ceramide synthase CSH1... [more]
sp|Q9UT67|IMT2_SCHPO8.833e-2043.52Inositol phosphoceramide mannosyltransferase 2 OS=... [more]
sp|O14084|IMT1_SCHPO4.485e-1728.81Inositol phosphoceramide mannosyltransferase 1 OS=... [more]
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BLAST of inositol phosphoceramide mannosyltransferase 2-like vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1I8H5Y81.261e-5338.96Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8HSW65.071e-5338.71Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267F5S56.460e-5338.96Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267F5L21.601e-5238.55Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8FXP51.798e-5238.10Uncharacterized protein OS=Macrostomum lignano OX=... [more]
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BLAST of inositol phosphoceramide mannosyltransferase 2-like vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of inositol phosphoceramide mannosyltransferase 2-like vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of inositol phosphoceramide mannosyltransferase 2-like vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 3
Match NameE-valueIdentityDescription
SUR16.968e-2334.00Mannosylinositol phosphorylceramide (MIPC) synthas... [more]
CSH14.840e-2235.33Mannosylinositol phosphorylceramide (MIPC) synthas... [more]
OCH19.027e-930.95Mannosyltransferase of the cis-Golgi apparatus; in... [more]
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BLAST of inositol phosphoceramide mannosyltransferase 2-like vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of inositol phosphoceramide mannosyltransferase 2-like vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of inositol phosphoceramide mannosyltransferase 2-like vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000045480.10.000e+091.20SMESG000045480.1[more]
SMESG000045292.10.000e+088.73SMESG000045292.1[more]
SMESG000045267.10.000e+090.85SMESG000045267.1[more]
SMESG000037990.10.000e+088.64SMESG000037990.1[more]
SMESG000059812.12.759e-149100.00SMESG000059812.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30019717 ID=SMED30019717|Name=inositol phosphoceramide mannosyltransferase 2-like|organism=Schmidtea mediterranea sexual|type=transcript|length=1126bp
TTGCTTTATTTTTTTATGCCAAGGAAATATCTGTTATAGAATATTTGCAG
TATCGACAACGCCTTTAAGCTTGAAAGAAAAATTTTGAAAAATACATACA
TACAATTTCTGTAATCAAAATATTTCTTTCTCAGACAACCAATGCATGAC
CTGTAGTGTGTGCATGTTAACTGACCAATCAAATCATAGTATTTTAAATG
AGTACATTTATTATGAGTAAATTTATGAAGATTTTTTTTCTACTTACAAG
TGCCATCTTTATAGGAATTTTCTTTGTTGAAAATTATGAACGATTTTACA
TTGTCAAACAATCGATTCAAATCCAATTGAACAATTGTGAAAATGAAAAT
GTGACGAAAATTATCCATGTTTCTTGGAAGACAAATGATATTCCAGTAGT
GTTTGAAAAGTATTTAAAATCATGGACAGATTTTTGTTACAAAGATTGGA
AATTCATGTTCTGGACAGATGAGGACAATTATAACTTGATCAAAACCTTT
TATCCATGGTTTCTTAAAAAGTACAACGAATTTCCTAGGAATATTTATCG
AGCTGATGTCAGTCGGTATTTCTATTTGTTTCATTATGGAGGAATTTACA
CCGATCTTGACAATGAATGTTTGCGTCCATTTGAGCATTTGTTGCAAAAC
TATTCTATCGTTTTCGGGGCTATGAAAGGTCAATATTTAGGGACCAATTT
AAAAGAAGGATACGTTCAAAATTCTTTCATGTATTCAAGACCGAGACAGA
CATTTTGGATGGAGTTGTTGAATGATATTCTCATGTTTAAAGGCACAAAT
TCTGTGGAAGTTCAAACTGGACCAGTTTTATTATCAAAACGAATCGTCTT
ATTTCGAGAACGGTTAAATTCCTCGGATTACATGATGATTTATTCTCCGA
ATTATTTCAACCCATTCAGTTGGATAGATAAATCAAAAGTGGACCCAAAT
TGTAAATCTCATAACCAAATGACTCGGGAGAATTTAAACAAATGTAGAAG
CTCCATGATTTCAAAGGGATCATATGTCGTTCAGTATCATGCCCACAGTT
GGGGATAAATTCTTAGAACTATAATAAATTCTTAGATTGTGTTTTGTAAA
TATATTTTAACGTCACTAAAAAAAAA
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protein sequence of SMED30019717-orf-1

>SMED30019717-orf-1 ID=SMED30019717-orf-1|Name=SMED30019717-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=287bp
MSTFIMSKFMKIFFLLTSAIFIGIFFVENYERFYIVKQSIQIQLNNCENE
NVTKIIHVSWKTNDIPVVFEKYLKSWTDFCYKDWKFMFWTDEDNYNLIKT
FYPWFLKKYNEFPRNIYRADVSRYFYLFHYGGIYTDLDNECLRPFEHLLQ
NYSIVFGAMKGQYLGTNLKEGYVQNSFMYSRPRQTFWMELLNDILMFKGT
NSVEVQTGPVLLSKRIVLFRERLNSSDYMMIYSPNYFNPFSWIDKSKVDP
NCKSHNQMTRENLNKCRSSMISKGSYVVQYHAHSWG*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000018Category 4 cell
PLANA:0000026gut
PLANA:0000034epidermis
PLANA:0000418head
PLANA:0002032epidermal cell
Vocabulary: INTERPRO
TermDefinition
IPR007577GlycoTrfase_DXD_sugar-bd_CS
IPR012336Thioredoxin-like_fold
IPR029044Nucleotide-diphossugar_trans
Vocabulary: molecular function
TermDefinition
GO:0000030mannosyltransferase activity
GO:0016740transferase activity
GO:0016757transferase activity, transferring glycosyl groups
Vocabulary: cellular component
TermDefinition
GO:0005783endoplasmic reticulum
GO:0005789endoplasmic reticulum membrane
GO:0005794Golgi apparatus
GO:0016020membrane
GO:0016021integral component of membrane
GO:0032588trans-Golgi network membrane
GO:0033106cis-Golgi network membrane
Vocabulary: biological process
TermDefinition
GO:0051999mannosyl-inositol phosphorylceramide biosynthetic process
GO:0097502mannosylation
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:3.90.550.20coord: 22..147
e-value: 1.4E-7
score: 33.2
NoneNo IPR availablePANTHERPTHR32385FAMILY NOT NAMEDcoord: 1..200
IPR007577Glycosyltransferase, DXD sugar-binding motifPFAMPF04488Gly_transf_sugcoord: 8..66
e-value: 1.1E-13
score: 51.6
IPR029044Nucleotide-diphospho-sugar transferasesSUPERFAMILYSSF53448Nucleotide-diphospho-sugar transferasescoord: 2..164