SMED30019683
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30019683 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 11
Homology
BLAST of SMED30019683 vs. TrEMBL
Match: A0A4Z2CL13 (Ephrin RBD domain-containing protein OS=Schistosoma japonicum OX=6182 GN=EWB00_009928 PE=4 SV=1) HSP 1 Score: 77.0258 bits (188), Expect = 3.842e-12 Identity = 37/136 (27.21%), Postives = 72/136 (52.94%), Query Frame = 2 Query: 293 VVMLLFGIPSASTQRTHRLFWDDKNPVFNPGNNRMFVEEGDDITIVCPSNSPEHNNIYWSKKNEVFDLCEPTFDSNSVMMIHC-EPYSIKEFNFQVTKFSWVSLNPSFLRNKPIYLLSSPKVCKNYNMKIAIILKT 697 ++M+L + A Q H ++WD +P F +N ++V +GD++ +C N N+YW+K V C T + ++ C S+ EF +V++F ++ PSF N P++ ++ +C+ N +++ LK+ Sbjct: 10 IIMILIKLTCAEHQ-DHIIYWDASHPRFKGNHNEIYVNDGDNLIFICSQNLANVQNLYWTKDPRVQMKCNKTLQIKVIQLLDCFSKKSLNEFTLKVSRFPEITSLPSFDYNIPVHFIAHSVICQKNNFRLSFKLKS 144
BLAST of SMED30019683 vs. TrEMBL
Match: A0A3Q0KLD0 (Ephrin RBD domain-containing protein OS=Schistosoma mansoni OX=6183 PE=4 SV=1) HSP 1 Score: 73.559 bits (179), Expect = 1.555e-10 Identity = 37/120 (30.83%), Postives = 61/120 (50.83%), Query Frame = 2 Query: 335 RTHRLFWDDKNPVFNPGNNRMFVEEGDDITIVCPSNSPEHNNIYWSKKNEVFDLCEPTFDSNSVMMIHC-EPYSIKEFNFQVTKFSWVSLNPSFLRNKPIYLLSSPKVCKNYNMKIAIIL 691 + H + WD N +F+ V+EGD+I VCPS+ ++W+ E +D C S +V ++ C + EF +V +FS + +P F N +Y L +C YN++IA+ L Sbjct: 23 QDHIIMWDITNKLFSSNVTMFRVKEGDNIVFVCPSDVRRSQKLFWTMSKETYDECGAPSSSQTVKLLDCSQSPKNTEFILKVAQFSEIPYSPHFRENDFVYFLGQHDLCSIYNLRIAVKL 142
BLAST of SMED30019683 vs. TrEMBL
Match: A0A3Q0KM31 (Ephrin RBD domain-containing protein OS=Schistosoma mansoni OX=6183 PE=4 SV=1) HSP 1 Score: 72.7886 bits (177), Expect = 2.632e-10 Identity = 36/140 (25.71%), Postives = 72/140 (51.43%), Query Frame = 2 Query: 293 VVMLLFGIPSASTQRTHRLFWDDKNPVFNPGNNRMFVEEGDDITIVCPSNSPEHNNIYWSKKNEVFDLCEPTFDSNSVMMIHC--EPYSIKEFNFQVTKFSWVSLNPSFLRNKPIYLLSSPKVCKNYNMKIAIILKTFEK 706 ++++ I + + + H + WD N F G N ++V EGD++ +CP N ++YW+ + V C T + ++ C + Y+ EF +++KF +S P F PI+ ++ +C+N N ++++ L + K Sbjct: 9 IIIMQSLICTCAQLQDHVILWDASNWRFKEGQNEIYVNEGDNLIFICPPNRTFSQSLYWTNDSRVTIKCNRTLSIKVIKLLDCFGDNYAT-EFILKISKFHEISSLPVFHHELPIHFVAQSVICQNSNFRLSVRLASTHK 147
BLAST of SMED30019683 vs. TrEMBL
Match: A0A4Z2CSR2 (Ephrin RBD domain-containing protein OS=Schistosoma japonicum OX=6182 GN=EWB00_007939 PE=4 SV=1) HSP 1 Score: 71.633 bits (174), Expect = 6.978e-10 Identity = 42/154 (27.27%), Postives = 77/154 (50.00%), Query Frame = 2 Query: 287 MIVVMLLFGIPSASTQ-RTHRLFWDDKNPVFNPGNNRMFVEEGDDITIVCPSNSPEHNNIYWSKKNEVFDLCEPTFDSNSVMMIHC--EPYSIKEFNFQVTKFSWVSLNPSFLRNKPIYLLSSPKVCKNYNMKIAIILKTFEKEKVGILKGASP 739 +I ++++ S STQ + H + WD N F N + V EGD++ +CP N ++YW+ + V C T + ++ C + Y+ EF +V++F +S P F N PIY ++ +C+ N ++++ L + K + +SP Sbjct: 6 IIGIIIMQSFISTSTQFQDHIILWDASNWRFKEHQNEINVNEGDNLIFICPPNRTFSQSLYWTNDSRVTIKCNRTSPIKVIKLLDCFGDKYA-SEFILKVSRFHEISSLPVFHHNLPIYFVAQSVICQTSNFRLSVKLASAHINKSTVTISSSP 158
BLAST of SMED30019683 vs. TrEMBL
Match: A0A4Z2CSE1 (Ephrin RBD domain-containing protein OS=Schistosoma japonicum OX=6182 GN=EWB00_007937 PE=4 SV=1) HSP 1 Score: 70.8626 bits (172), Expect = 1.167e-9 Identity = 41/160 (25.62%), Postives = 75/160 (46.88%), Query Frame = 2 Query: 290 IVVMLLFGIPSASTQRTHRLFWDDKNPVFNPGNNRMFVEEGDDITIVCPSNSPEHNNIYWSKKNEVFDLCEPTFDSNSVMMIHC-EPYSIKEFNFQVTKFSWVSLNPSFLRNKPIYLLSSPKVCKNYNMKIAIILKTFEKEKVGILKGASPERVVQQKNS 766 I VM+L S R H + WD N F N ++ EGD++ +CP N N+YW+ + + C T + ++ C S+ EF +V++F ++ PSF P+Y ++ +C+ N ++++ L + + K S E+ + +S Sbjct: 9 ITVMILIEFTSTEL-RDHIILWDASNWRFKENQNNLYANEGDNLIFICPKNRTFSQNLYWTNDSRLQLKCNKTMPIKVIKLLDCYGNKSVTEFILKVSRFPEIASLPSFHPEIPVYFVAQSVICQQNNFRLSVKLMSDNMTNITEQKVNSNEKQTMKHSS 167
BLAST of SMED30019683 vs. Planmine SMEST
Match: SMESG000031186.1 (SMESG000031186.1) HSP 1 Score: 491.5 bits (1264), Expect = 7.747e-177 Identity = 238/240 (99.17%), Postives = 240/240 (100.00%), Query Frame = 2 Query: 110 MKSSSVIHGPGNGKCTKLSLTSFNVIKTERKYFTLSISIFITFHNFMIRSVYDFASVSNMIVVMLLFGIPSASTQRTHRLFWDDKNPVFNPGNNRMFVEEGDDITIVCPSNSPEHNNIYWSKKNEVFDLCEPTFDSNSVMMIHCEPYSIKEFNFQVTKFSWVSLNPSFLRNKPIYLLSSPKVCKNYNMKIAIILKTFEKEKVGILKGASPERVVQQKNSAPVPLLSLALTISLFKWSLFY 829 MKSSSVIHGPGNGKCTKLSLTSFNVIKTERKYFTLSISIFITFHNFMIRSVYDFASVSNMIV+MLLFGIPSASTQRTHRLFWDDKNPVFNPGNNRMFVEEGDDITIVCPSNSPEHNNIYWSKKNEVFDLCEPTFDSNS+MMIHCEPYSIKEFNFQVTKFSWVSLNPSFLRNKPIYLLSSPKVCKNYNMKIAIILKTFEKEKVGILKGASPERVVQQKNSAPVPLLSLALTISLFKWSLFY Sbjct: 1 MKSSSVIHGPGNGKCTKLSLTSFNVIKTERKYFTLSISIFITFHNFMIRSVYDFASVSNMIVIMLLFGIPSASTQRTHRLFWDDKNPVFNPGNNRMFVEEGDDITIVCPSNSPEHNNIYWSKKNEVFDLCEPTFDSNSIMMIHCEPYSIKEFNFQVTKFSWVSLNPSFLRNKPIYLLSSPKVCKNYNMKIAIILKTFEKEKVGILKGASPERVVQQKNSAPVPLLSLALTISLFKWSLFY 240
BLAST of SMED30019683 vs. Planmine SMEST
Match: SMESG000031186.1 (SMESG000031186.1) HSP 1 Score: 487.263 bits (1253), Expect = 4.102e-175 Identity = 238/241 (98.76%), Postives = 240/241 (99.59%), Query Frame = 2 Query: 110 MKSSSVIHGPGNGKCTKLSLTSFNVIKTERKYFTLSISIFITFHNFMIRSVYDFASVSNMIVVMLLFGIPSASTQRTHRLFWDDKNPVFNPGNNRMFVEEGDDITIVCPSNSPEHNNIYWSKKNEVFDLCEPTFDSNSVMMIHCEPYSIKEFNFQVTKFSWVSLNPSFLRNKPIYLLSSPKVCKNYNMKIAIILKTFEKEKVGILKG-ASPERVVQQKNSAPVPLLSLALTISLFKWSLFY 829 MKSSSVIHGPGNGKCTKLSLTSFNVIKTERKYFTLSISIFITFHNFMIRSVYDFASVSNMIV+MLLFGIPSASTQRTHRLFWDDKNPVFNPGNNRMFVEEGDDITIVCPSNSPEHNNIYWSKKNEVFDLCEPTFDSNS+MMIHCEPYSIKEFNFQVTKFSWVSLNPSFLRNKPIYLLSSPKVCKNYNMKIAIILKTFEKEKVGILKG ASPERVVQQKNSAPVPLLSLALTISLFKWSLFY Sbjct: 1 MKSSSVIHGPGNGKCTKLSLTSFNVIKTERKYFTLSISIFITFHNFMIRSVYDFASVSNMIVIMLLFGIPSASTQRTHRLFWDDKNPVFNPGNNRMFVEEGDDITIVCPSNSPEHNNIYWSKKNEVFDLCEPTFDSNSIMMIHCEPYSIKEFNFQVTKFSWVSLNPSFLRNKPIYLLSSPKVCKNYNMKIAIILKTFEKEKVGILKGAASPERVVQQKNSAPVPLLSLALTISLFKWSLFY 241
BLAST of SMED30019683 vs. Planmine SMEST
Match: SMESG000063175.1 (SMESG000063175.1) HSP 1 Score: 85.1149 bits (209), Expect = 3.959e-19 Identity = 46/138 (33.33%), Postives = 73/138 (52.90%), Query Frame = 2 Query: 290 IVVMLLFGIPSASTQ----RTHRLFWDDKNPVFNPGNNRMFVEEGDDITIVCPSNSPEHNNIYWSKKNEVFDLCEPTFDSNSVMMIHCEPYSIKEFNFQVTKFSWVSLNPSFLRNKPIYLLSSPKVCKNYNMKIAIIL 691 + +++LF +A + H + W +P+F N + V D+I I CP E IYWS F C+P + + C P +K F+ VT+FSWVSLNP+F +N+P+Y ++ C+ YNM++ II+ Sbjct: 17 VYILILFRYSNAHDSSLPPKEHIIEWKKFSPLFERSANILKVNHDDNILIKCPVLENEIFAIYWSYDRNDFIKCDPEDSLTTTPLFTCNP--MKSFSLLVTRFSWVSLNPTFSQNRPVYFIAQRSYCQGYNMRLRIIV 152
BLAST of SMED30019683 vs. Planmine SMEST
Match: SMESG000029274.1 (SMESG000029274.1) HSP 1 Score: 79.7221 bits (195), Expect = 2.856e-17 Identity = 42/153 (27.45%), Postives = 81/153 (52.94%), Query Frame = 2 Query: 281 SNMIVVMLLFGIPSASTQRTHRLFWDDKNPVFNPGNNRMFVEEGDDITIVCPSNSPEHNNIY--WSKKNEVFDLCEPTFDSNSV---MMIHCEPYSIK-EFNFQVTKFSWVSLNPSFLRNKPIYLLSSPKVCKNYNMKIAIILKTFEKEKVGI 721 +++ V+ L F + ++ +H ++W NP+F +N + V++GD+I +VCP + IY W+ + C ++ S ++ +C + +F QV+ +SWV NP+F +P+Y + ++C+NY MK+ IL+ E + I Sbjct: 5 ASLAVISLFFDLVISTV--SHNIYWSPHNPLFKISSNHLIVKDGDNIELVCPKTYDIVDKIYIFWTTNYNTYQNCGANSEAESEDLHLLFNCSLDRLNPQFRLQVSIYSWVISNPAFSNKQPVYFIPQRELCENYGMKLVTILQEDRNEMISI 155
BLAST of SMED30019683 vs. Planmine SMEST
Match: SMESG000029274.1 (SMESG000029274.1) HSP 1 Score: 79.7221 bits (195), Expect = 4.035e-17 Identity = 42/153 (27.45%), Postives = 81/153 (52.94%), Query Frame = 2 Query: 281 SNMIVVMLLFGIPSASTQRTHRLFWDDKNPVFNPGNNRMFVEEGDDITIVCPSNSPEHNNIY--WSKKNEVFDLCEPTFDSNSV---MMIHCEPYSIK-EFNFQVTKFSWVSLNPSFLRNKPIYLLSSPKVCKNYNMKIAIILKTFEKEKVGI 721 +++ V+ L F + ++ +H ++W NP+F +N + V++GD+I +VCP + IY W+ + C ++ S ++ +C + +F QV+ +SWV NP+F +P+Y + ++C+NY MK+ IL+ E + I Sbjct: 10 ASLAVISLFFDLVISTV--SHNIYWSPHNPLFKISSNHLIVKDGDNIELVCPKTYDIVDKIYIFWTTNYNTYQNCGANSEAESEDLHLLFNCSLDRLNPQFRLQVSIYSWVISNPAFSNKQPVYFIPQRELCENYGMKLVTILQEDRNEMISI 160 The following BLAST results are available for this feature:
BLAST of SMED30019683 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30019683 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30019683 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30019683 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30019683 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30019683 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30019683 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30019683 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30019683 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30019683 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30019683 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30019683 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30019683 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30019683 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30019683 ID=SMED30019683|Name=SMED30019683|organism=Schmidtea mediterranea sexual|type=transcript|length=1132bpback to top protein sequence of SMED30019683-orf-1 >SMED30019683-orf-1 ID=SMED30019683-orf-1|Name=SMED30019683-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=241bp MKSSSVIHGPGNGKCTKLSLTSFNVIKTERKYFTLSISIFITFHNFMIRSback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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