SMED30019683

Overview
NameSMED30019683
Smed IDSMED30019683
Length (bp)1132
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30019683 (SMED30019683) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30019683 (SMED30019683) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30019683 (SMED30019683) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30019683 (SMED30019683) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30019683

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 11

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30019683h1SMcG0003602 dd_Smed_v4_10687_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30019683h1SMcG0003602 dd_Smed_v4_10687_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30019683h1SMcG0003602 dd_Smed_v4_10687_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult colorimetric in situ hybridization evidence
muscle cellSMED30019683h1SMcG0003602 dd_Smed_v4_10687_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30019683h1SMcG0003602 dd_Smed_v4_10687_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30019683h1SMcG0003602 dd_Smed_v4_10687_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
posterior sideSMED30019683h1SMcG0003602 dd_Smed_v4_10687_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
posterior muscle cellSMED30019683h1SMcG0003602 dd_Smed_v4_10687_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30019683h1SMcG0003602 dd_Smed_v6_10687_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
body wall musculatureSMED30019683h1SMcG0003602 dd_Smed_v4_10687_0_1dd_Smed_v4PMID:30471994
Scimone et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30019683h1SMcG0003602 dd_Smed_v4_10687_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of SMED30019683 vs. TrEMBL
Match: A0A4Z2CL13 (Ephrin RBD domain-containing protein OS=Schistosoma japonicum OX=6182 GN=EWB00_009928 PE=4 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 3.842e-12
Identity = 37/136 (27.21%), Postives = 72/136 (52.94%), Query Frame = 2
Query:  293 VVMLLFGIPSASTQRTHRLFWDDKNPVFNPGNNRMFVEEGDDITIVCPSNSPEHNNIYWSKKNEVFDLCEPTFDSNSVMMIHC-EPYSIKEFNFQVTKFSWVSLNPSFLRNKPIYLLSSPKVCKNYNMKIAIILKT 697
            ++M+L  +  A  Q  H ++WD  +P F   +N ++V +GD++  +C  N     N+YW+K   V   C  T     + ++ C    S+ EF  +V++F  ++  PSF  N P++ ++   +C+  N +++  LK+
Sbjct:   10 IIMILIKLTCAEHQ-DHIIYWDASHPRFKGNHNEIYVNDGDNLIFICSQNLANVQNLYWTKDPRVQMKCNKTLQIKVIQLLDCFSKKSLNEFTLKVSRFPEITSLPSFDYNIPVHFIAHSVICQKNNFRLSFKLKS 144          
BLAST of SMED30019683 vs. TrEMBL
Match: A0A3Q0KLD0 (Ephrin RBD domain-containing protein OS=Schistosoma mansoni OX=6183 PE=4 SV=1)

HSP 1 Score: 73.559 bits (179), Expect = 1.555e-10
Identity = 37/120 (30.83%), Postives = 61/120 (50.83%), Query Frame = 2
Query:  335 RTHRLFWDDKNPVFNPGNNRMFVEEGDDITIVCPSNSPEHNNIYWSKKNEVFDLCEPTFDSNSVMMIHC-EPYSIKEFNFQVTKFSWVSLNPSFLRNKPIYLLSSPKVCKNYNMKIAIIL 691
            + H + WD  N +F+       V+EGD+I  VCPS+      ++W+   E +D C     S +V ++ C +     EF  +V +FS +  +P F  N  +Y L    +C  YN++IA+ L
Sbjct:   23 QDHIIMWDITNKLFSSNVTMFRVKEGDNIVFVCPSDVRRSQKLFWTMSKETYDECGAPSSSQTVKLLDCSQSPKNTEFILKVAQFSEIPYSPHFRENDFVYFLGQHDLCSIYNLRIAVKL 142          
BLAST of SMED30019683 vs. TrEMBL
Match: A0A3Q0KM31 (Ephrin RBD domain-containing protein OS=Schistosoma mansoni OX=6183 PE=4 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 2.632e-10
Identity = 36/140 (25.71%), Postives = 72/140 (51.43%), Query Frame = 2
Query:  293 VVMLLFGIPSASTQRTHRLFWDDKNPVFNPGNNRMFVEEGDDITIVCPSNSPEHNNIYWSKKNEVFDLCEPTFDSNSVMMIHC--EPYSIKEFNFQVTKFSWVSLNPSFLRNKPIYLLSSPKVCKNYNMKIAIILKTFEK 706
            ++++   I + +  + H + WD  N  F  G N ++V EGD++  +CP N     ++YW+  + V   C  T     + ++ C  + Y+  EF  +++KF  +S  P F    PI+ ++   +C+N N ++++ L +  K
Sbjct:    9 IIIMQSLICTCAQLQDHVILWDASNWRFKEGQNEIYVNEGDNLIFICPPNRTFSQSLYWTNDSRVTIKCNRTLSIKVIKLLDCFGDNYAT-EFILKISKFHEISSLPVFHHELPIHFVAQSVICQNSNFRLSVRLASTHK 147          
BLAST of SMED30019683 vs. TrEMBL
Match: A0A4Z2CSR2 (Ephrin RBD domain-containing protein OS=Schistosoma japonicum OX=6182 GN=EWB00_007939 PE=4 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 6.978e-10
Identity = 42/154 (27.27%), Postives = 77/154 (50.00%), Query Frame = 2
Query:  287 MIVVMLLFGIPSASTQ-RTHRLFWDDKNPVFNPGNNRMFVEEGDDITIVCPSNSPEHNNIYWSKKNEVFDLCEPTFDSNSVMMIHC--EPYSIKEFNFQVTKFSWVSLNPSFLRNKPIYLLSSPKVCKNYNMKIAIILKTFEKEKVGILKGASP 739
            +I ++++    S STQ + H + WD  N  F    N + V EGD++  +CP N     ++YW+  + V   C  T     + ++ C  + Y+  EF  +V++F  +S  P F  N PIY ++   +C+  N ++++ L +    K  +   +SP
Sbjct:    6 IIGIIIMQSFISTSTQFQDHIILWDASNWRFKEHQNEINVNEGDNLIFICPPNRTFSQSLYWTNDSRVTIKCNRTSPIKVIKLLDCFGDKYA-SEFILKVSRFHEISSLPVFHHNLPIYFVAQSVICQTSNFRLSVKLASAHINKSTVTISSSP 158          
BLAST of SMED30019683 vs. TrEMBL
Match: A0A4Z2CSE1 (Ephrin RBD domain-containing protein OS=Schistosoma japonicum OX=6182 GN=EWB00_007937 PE=4 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 1.167e-9
Identity = 41/160 (25.62%), Postives = 75/160 (46.88%), Query Frame = 2
Query:  290 IVVMLLFGIPSASTQRTHRLFWDDKNPVFNPGNNRMFVEEGDDITIVCPSNSPEHNNIYWSKKNEVFDLCEPTFDSNSVMMIHC-EPYSIKEFNFQVTKFSWVSLNPSFLRNKPIYLLSSPKVCKNYNMKIAIILKTFEKEKVGILKGASPERVVQQKNS 766
            I VM+L    S    R H + WD  N  F    N ++  EGD++  +CP N     N+YW+  + +   C  T     + ++ C    S+ EF  +V++F  ++  PSF    P+Y ++   +C+  N ++++ L +     +   K  S E+   + +S
Sbjct:    9 ITVMILIEFTSTEL-RDHIILWDASNWRFKENQNNLYANEGDNLIFICPKNRTFSQNLYWTNDSRLQLKCNKTMPIKVIKLLDCYGNKSVTEFILKVSRFPEIASLPSFHPEIPVYFVAQSVICQQNNFRLSVKLMSDNMTNITEQKVNSNEKQTMKHSS 167          
BLAST of SMED30019683 vs. Planmine SMEST
Match: SMESG000031186.1 (SMESG000031186.1)

HSP 1 Score: 491.5 bits (1264), Expect = 7.747e-177
Identity = 238/240 (99.17%), Postives = 240/240 (100.00%), Query Frame = 2
Query:  110 MKSSSVIHGPGNGKCTKLSLTSFNVIKTERKYFTLSISIFITFHNFMIRSVYDFASVSNMIVVMLLFGIPSASTQRTHRLFWDDKNPVFNPGNNRMFVEEGDDITIVCPSNSPEHNNIYWSKKNEVFDLCEPTFDSNSVMMIHCEPYSIKEFNFQVTKFSWVSLNPSFLRNKPIYLLSSPKVCKNYNMKIAIILKTFEKEKVGILKGASPERVVQQKNSAPVPLLSLALTISLFKWSLFY 829
            MKSSSVIHGPGNGKCTKLSLTSFNVIKTERKYFTLSISIFITFHNFMIRSVYDFASVSNMIV+MLLFGIPSASTQRTHRLFWDDKNPVFNPGNNRMFVEEGDDITIVCPSNSPEHNNIYWSKKNEVFDLCEPTFDSNS+MMIHCEPYSIKEFNFQVTKFSWVSLNPSFLRNKPIYLLSSPKVCKNYNMKIAIILKTFEKEKVGILKGASPERVVQQKNSAPVPLLSLALTISLFKWSLFY
Sbjct:    1 MKSSSVIHGPGNGKCTKLSLTSFNVIKTERKYFTLSISIFITFHNFMIRSVYDFASVSNMIVIMLLFGIPSASTQRTHRLFWDDKNPVFNPGNNRMFVEEGDDITIVCPSNSPEHNNIYWSKKNEVFDLCEPTFDSNSIMMIHCEPYSIKEFNFQVTKFSWVSLNPSFLRNKPIYLLSSPKVCKNYNMKIAIILKTFEKEKVGILKGASPERVVQQKNSAPVPLLSLALTISLFKWSLFY 240          
BLAST of SMED30019683 vs. Planmine SMEST
Match: SMESG000031186.1 (SMESG000031186.1)

HSP 1 Score: 487.263 bits (1253), Expect = 4.102e-175
Identity = 238/241 (98.76%), Postives = 240/241 (99.59%), Query Frame = 2
Query:  110 MKSSSVIHGPGNGKCTKLSLTSFNVIKTERKYFTLSISIFITFHNFMIRSVYDFASVSNMIVVMLLFGIPSASTQRTHRLFWDDKNPVFNPGNNRMFVEEGDDITIVCPSNSPEHNNIYWSKKNEVFDLCEPTFDSNSVMMIHCEPYSIKEFNFQVTKFSWVSLNPSFLRNKPIYLLSSPKVCKNYNMKIAIILKTFEKEKVGILKG-ASPERVVQQKNSAPVPLLSLALTISLFKWSLFY 829
            MKSSSVIHGPGNGKCTKLSLTSFNVIKTERKYFTLSISIFITFHNFMIRSVYDFASVSNMIV+MLLFGIPSASTQRTHRLFWDDKNPVFNPGNNRMFVEEGDDITIVCPSNSPEHNNIYWSKKNEVFDLCEPTFDSNS+MMIHCEPYSIKEFNFQVTKFSWVSLNPSFLRNKPIYLLSSPKVCKNYNMKIAIILKTFEKEKVGILKG ASPERVVQQKNSAPVPLLSLALTISLFKWSLFY
Sbjct:    1 MKSSSVIHGPGNGKCTKLSLTSFNVIKTERKYFTLSISIFITFHNFMIRSVYDFASVSNMIVIMLLFGIPSASTQRTHRLFWDDKNPVFNPGNNRMFVEEGDDITIVCPSNSPEHNNIYWSKKNEVFDLCEPTFDSNSIMMIHCEPYSIKEFNFQVTKFSWVSLNPSFLRNKPIYLLSSPKVCKNYNMKIAIILKTFEKEKVGILKGAASPERVVQQKNSAPVPLLSLALTISLFKWSLFY 241          
BLAST of SMED30019683 vs. Planmine SMEST
Match: SMESG000063175.1 (SMESG000063175.1)

HSP 1 Score: 85.1149 bits (209), Expect = 3.959e-19
Identity = 46/138 (33.33%), Postives = 73/138 (52.90%), Query Frame = 2
Query:  290 IVVMLLFGIPSASTQ----RTHRLFWDDKNPVFNPGNNRMFVEEGDDITIVCPSNSPEHNNIYWSKKNEVFDLCEPTFDSNSVMMIHCEPYSIKEFNFQVTKFSWVSLNPSFLRNKPIYLLSSPKVCKNYNMKIAIIL 691
            + +++LF   +A       + H + W   +P+F    N + V   D+I I CP    E   IYWS     F  C+P     +  +  C P  +K F+  VT+FSWVSLNP+F +N+P+Y ++    C+ YNM++ II+
Sbjct:   17 VYILILFRYSNAHDSSLPPKEHIIEWKKFSPLFERSANILKVNHDDNILIKCPVLENEIFAIYWSYDRNDFIKCDPEDSLTTTPLFTCNP--MKSFSLLVTRFSWVSLNPTFSQNRPVYFIAQRSYCQGYNMRLRIIV 152          
BLAST of SMED30019683 vs. Planmine SMEST
Match: SMESG000029274.1 (SMESG000029274.1)

HSP 1 Score: 79.7221 bits (195), Expect = 2.856e-17
Identity = 42/153 (27.45%), Postives = 81/153 (52.94%), Query Frame = 2
Query:  281 SNMIVVMLLFGIPSASTQRTHRLFWDDKNPVFNPGNNRMFVEEGDDITIVCPSNSPEHNNIY--WSKKNEVFDLCEPTFDSNSV---MMIHCEPYSIK-EFNFQVTKFSWVSLNPSFLRNKPIYLLSSPKVCKNYNMKIAIILKTFEKEKVGI 721
            +++ V+ L F +  ++   +H ++W   NP+F   +N + V++GD+I +VCP      + IY  W+     +  C    ++ S    ++ +C    +  +F  QV+ +SWV  NP+F   +P+Y +   ++C+NY MK+  IL+    E + I
Sbjct:    5 ASLAVISLFFDLVISTV--SHNIYWSPHNPLFKISSNHLIVKDGDNIELVCPKTYDIVDKIYIFWTTNYNTYQNCGANSEAESEDLHLLFNCSLDRLNPQFRLQVSIYSWVISNPAFSNKQPVYFIPQRELCENYGMKLVTILQEDRNEMISI 155          
BLAST of SMED30019683 vs. Planmine SMEST
Match: SMESG000029274.1 (SMESG000029274.1)

HSP 1 Score: 79.7221 bits (195), Expect = 4.035e-17
Identity = 42/153 (27.45%), Postives = 81/153 (52.94%), Query Frame = 2
Query:  281 SNMIVVMLLFGIPSASTQRTHRLFWDDKNPVFNPGNNRMFVEEGDDITIVCPSNSPEHNNIY--WSKKNEVFDLCEPTFDSNSV---MMIHCEPYSIK-EFNFQVTKFSWVSLNPSFLRNKPIYLLSSPKVCKNYNMKIAIILKTFEKEKVGI 721
            +++ V+ L F +  ++   +H ++W   NP+F   +N + V++GD+I +VCP      + IY  W+     +  C    ++ S    ++ +C    +  +F  QV+ +SWV  NP+F   +P+Y +   ++C+NY MK+  IL+    E + I
Sbjct:   10 ASLAVISLFFDLVISTV--SHNIYWSPHNPLFKISSNHLIVKDGDNIELVCPKTYDIVDKIYIFWTTNYNTYQNCGANSEAESEDLHLLFNCSLDRLNPQFRLQVSIYSWVISNPAFSNKQPVYFIPQRELCENYGMKLVTILQEDRNEMISI 160          
The following BLAST results are available for this feature:
BLAST of SMED30019683 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019683 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019683 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019683 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019683 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019683 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019683 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019683 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A4Z2CL133.842e-1227.21Ephrin RBD domain-containing protein OS=Schistosom... [more]
A0A3Q0KLD01.555e-1030.83Ephrin RBD domain-containing protein OS=Schistosom... [more]
A0A3Q0KM312.632e-1025.71Ephrin RBD domain-containing protein OS=Schistosom... [more]
A0A4Z2CSR26.978e-1027.27Ephrin RBD domain-containing protein OS=Schistosom... [more]
A0A4Z2CSE11.167e-925.63Ephrin RBD domain-containing protein OS=Schistosom... [more]
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BLAST of SMED30019683 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019683 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019683 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019683 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019683 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019683 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000031186.17.747e-17799.17SMESG000031186.1[more]
SMESG000031186.14.102e-17598.76SMESG000031186.1[more]
SMESG000063175.13.959e-1933.33SMESG000063175.1[more]
SMESG000029274.12.856e-1727.45SMESG000029274.1[more]
SMESG000029274.14.035e-1727.45SMESG000029274.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30019683 ID=SMED30019683|Name=SMED30019683|organism=Schmidtea mediterranea sexual|type=transcript|length=1132bp
TCAAATTTAACATTCAAGTTTAATATATTAAAAAATTTTCAATCAAAATT
TTGAAAAAATTGTGGATCATACATCTGTTTGTCGGGTCTGCGATCATTTA
TCTAAATCTATGAAGTCGTCCTCAGTCATTCACGGTCCAGGCAATGGGAA
ATGTACCAAACTCTCACTAACTTCGTTTAACGTCATCAAAACTGAAAGAA
AATATTTCACTTTATCAATTTCTATTTTTATTACTTTCCACAACTTTATG
ATCAGATCGGTGTATGATTTTGCATCGGTATCAAATATGATCGTCGTTAT
GCTATTGTTCGGAATACCTTCTGCTTCAACTCAGCGTACTCACAGATTAT
TCTGGGATGACAAAAACCCTGTGTTTAATCCAGGAAATAACAGGATGTTT
GTGGAAGAAGGAGACGATATCACAATAGTTTGTCCATCAAATTCACCAGA
ACACAACAATATTTATTGGAGTAAGAAAAATGAAGTATTTGACTTATGTG
AACCTACATTCGATAGCAATTCCGTTATGATGATCCATTGTGAACCATAT
AGCATCAAGGAGTTCAATTTTCAAGTGACAAAATTTTCTTGGGTCAGTCT
CAATCCTTCATTTCTTCGGAATAAACCCATTTATTTATTATCATCACCGA
AAGTTTGTAAAAATTACAACATGAAAATTGCCATTATTCTGAAAACATTC
GAGAAAGAGAAAGTGGGAATATTGAAAGGAGCTTCACCAGAGAGAGTCGT
TCAACAGAAAAATTCTGCGCCAGTACCATTGCTATCATTAGCGTTAACGA
TTTCTTTGTTTAAATGGTCACTTTTCTACTGAGGATTTCAATCGCACATT
CGATATCTAGTCCCCGATCCTGTTCATATCATACCCGAGCACTACGAGAT
TGTGAATTATTATTAATATAAACCCACTATAAAATTGCAATTGACTACAT
TTAATAAACTCTACTCAATATACGATACTCAATGACAAAAATTTGAATAT
CAATGAAATGATGGAAATTTATATTTTATATAAATTTCATTCTTTTGAAC
CTGTTTTTCATGGGTTATTTGAGATTTCGGACTTGGATTTTTTATTTACT
TGAATAGAAAAGATGTTAATAGTTGAAAAAAA
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protein sequence of SMED30019683-orf-1

>SMED30019683-orf-1 ID=SMED30019683-orf-1|Name=SMED30019683-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=241bp
MKSSSVIHGPGNGKCTKLSLTSFNVIKTERKYFTLSISIFITFHNFMIRS
VYDFASVSNMIVVMLLFGIPSASTQRTHRLFWDDKNPVFNPGNNRMFVEE
GDDITIVCPSNSPEHNNIYWSKKNEVFDLCEPTFDSNSVMMIHCEPYSIK
EFNFQVTKFSWVSLNPSFLRNKPIYLLSSPKVCKNYNMKIAIILKTFEKE
KVGILKGASPERVVQQKNSAPVPLLSLALTISLFKWSLFY*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000034epidermis
PLANA:0000099neuron
PLANA:0000101muscle cell
PLANA:0000142posterior region of the whole animal
PLANA:0000240body wall musculature
PLANA:0000418head
PLANA:0003001lateral region of the whole animal
Vocabulary: INTERPRO
TermDefinition
IPR001799Ephrin_RBD
IPR008972Cupredoxin
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001799Ephrin receptor-binding domainPFAMPF00812Ephrincoord: 77..188
e-value: 1.6E-9
score: 37.9
IPR008972CupredoxinGENE3DG3DSA:2.60.40.420coord: 71..202
e-value: 4.3E-10
score: 41.1
IPR008972CupredoxinSUPERFAMILYSSF49503Cupredoxinscoord: 75..192