Proactivator polypeptide
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30019668 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 33Note: Hover over icons to view figure legend
Homology
BLAST of Proactivator polypeptide vs. Ensembl Human
Match: PSAP (prosaposin [Source:HGNC Symbol;Acc:HGNC:9498]) HSP 1 Score: 89.7373 bits (221), Expect = 6.098e-18 Identity = 116/531 (21.85%), Postives = 212/531 (39.92%), Query Frame = 2
Query: 1043 CGNHHVCTSHSIKKPIASEKGVIECDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHLK--SISSQCSSLVDAYADQLITMISNHMDSKA-ICIAINACKGF-----SISQRKSA-----PNFKGIECDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIFQSEKIQCQQLVDEFFDIIL------NFVLSELD-VDKVCKVMGMCSMKKVSGIECEVCERVMDEVIQLAFSNHSQQVVSNFIENKICPRLGFVKTECTSLIDTYLPVIFSLARSTLNSKVICAELKLCSLISQKKINLLMKSNINDDSAKCKICHFVVSELLTLLTNPDIQKSIENFLENHVCPHFGAKKQECINDINQYYSLVINIITEFLKPTL-CNELRLCNSTTTSIIMSQPLVKMSRAILSSNCQLCEKFYFHLQKIFLKN-TPISPLTVFANVCNLEDTDETNECLKHFRNLNSIVAAFHASRNIGEF-CKMTEMCKGSKKHHVGKKQCSWGPGFWCISRQHAAGCGPGAVKHCEMKVWN 2566
CG C KP + CD+C+ VT A + ++ +++ K C L ++S+ C +VD+Y ++ +I M +C A+N C+ ++ +K P E A +A + Y D R P + CQ + DI FV + ++ V + C +G G+ ++C+ + + ++A Q++ + +IC +GF + T +P + + SK + L+L I KK + KS++ C++C F+V E+ L+ N +K I + + + +EC ++ Y S +++I+ E + P L C+ L LC+ T + V +++ C++C+K +L + KN T L C+ +C + ++ F C C + K +G ++C WGP +WC + + AA C AV+HC+ VWN
Sbjct: 42 CGAVKHCLQTVWNKPTVKS---LPCDICKDVVTAAGDMLKDNATEEEILVYLE-KTCDWLPKPNMSASCKEIVDSYLPVILDIIKGEMSRPGEVCSALNLCESLQKHLAELNHQKQLESNKIPELDMTEVVAPFMANIPLLLYPQDGPRSK-----------PQPKDNGDVCQDCIQMVTDIQTAVRTNSTFVQALVEHVKEECDRLG-------PGM-ADICKNYISQYSEIAI-----QMMMHMQPKEICALVGFCDEVKEMPMQTLVP-------AKVASKNVIPALELVEPI--KKHEVPAKSDVY-----CEVCEFLVKEVTKLIDNNKTEKEILDAFDKMCSKLPKSLSEECQEVVDTYGSSILSILLEEVSPELVCSMLHLCSGTRLPALT----VHVTQPKDGGFCEVCKKLVGYLDRNLEKNSTKQEILAALEKGCSFLPDPYQKQCDQFVAEYEPVLIEILVEVMDPSFVCLKIGACPSAHKPLLGTEKCIWGPSYWCQNTETAAQC--NAVEHCKRHVWN 524 HSP 2 Score: 55.0694 bits (131), Expect = 4.844e-7 Identity = 86/438 (19.63%), Postives = 162/438 (36.99%), Query Frame = 2
Query: 581 CTKCKQVFQFLESMFSKVSPDEFKFLIESLICNQLP-SEVQSTCKLLLDGYVTQLYNLIKSLLD-GDRICPLIHLCSSENTQITLGSWQCQTCLDVIVVLRKDLAGVSAP--------------------------------EIVSLIKSNV-----------------CSKLNFFEKGICNSYVSNLVPTFVNELHSTLTPQQICGNHHVCTS--------------------------HSIKKPIASEKGVIECDLCEIGVTGAKSFISKPENMNKVKSFIKS--KICSHL-KSISSQCSSLVDAYADQLITMISNHMDSKAICIAINACKGF---SISQRKSAPNFKGIECDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIFQSEKIQCQQLVDEFFDIILNFVLSELDVDKVCKVMGMC--SMKKVSGIE 1639
C CK V M + +E + C+ LP + ++CK ++D Y+ + ++IK + +C ++LC E+ Q L Q L+ + D+ V AP ++V+ I++ V C +L IC +Y+S + ++ + P++IC C IKK K + C++CE V I +N K + + K+CS L KS+S +C +VD Y +++++ + + +C ++ C G +++ + P G C+ C V + + + K I L+ + + QC Q V E+ +++ ++ +D VC +G C + K + G E
Sbjct: 63 CDICKDVVTAAGDMLKDNATEEEILVYLEKTCDWLPKPNMSASCKEIVDSYLPVILDIIKGEMSRPGEVCSALNLC--ESLQKHLAELNHQKQLESNKIPELDMTEVVAPFMANIPLLLYPQDGPRSKPQPKDNGDVCQDCIQMVTDIQTAVRTNSTFVQALVEHVKEECDRLGPGMADICKNYISQYSEIAI-QMMMHMQPKEICALVGFCDEVKEMPMQTLVPAKVASKNVIPALELVEPIKKHEVPAKSDVYCEVCEFLVKEVTKLI---DNNKTEKEILDAFDKMCSKLPKSLSEECQEVVDTYGSSILSILLEEVSPELVCSMLHLCSGTRLPALTVHVTQPKDGGF-CEVCKKLVGYLDRNLEKNSTKQEILAALEKGCSFLPDPYQKQCDQFVAEYEPVLIEILVEVMDPSFVCLKIGACPSAHKPLLGTE 493
BLAST of Proactivator polypeptide vs. Ensembl Human
Match: PSAP (prosaposin [Source:HGNC Symbol;Acc:HGNC:9498]) HSP 1 Score: 86.6557 bits (213), Expect = 7.104e-17 Identity = 102/482 (21.16%), Postives = 186/482 (38.59%), Query Frame = 2
Query: 1370 PNFKGIECDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIFQSEKIQ--CQQLVDEFFDIILNFVLSELD-VDKVCKVMGMCS--------------------------------------------------MKKVSGIECEVCERVMDEVIQLAFSNHS---QQVVSNFIENKICPRLGFVKTE-CTSLIDTY--LPVIFSLARSTLNSKVICAELKLCSLISQKKINLLMKSNI-----------------NDDSAK----CKICHFVVSELLTLLTNPDIQKSIENFLENHVCPHFGAKKQECINDINQYYSLVINIITEFLKPTL-CNELRLCNSTTTSIIMSQPLVKMSRAILSSNCQLCEKFYFHLQKIFLKN-TPISPLTVFANVCNLEDTDETNECLKHFRNLNSIVAAFHASRNIGEF-CKMTEMCKGSKKHHVGKKQCSWGPGFWCISRQHAAGCGPGAVKHCEMKVWN 2566
P K + CD C VT + + D A + I +L+ + C + C+++VD + +IL+ + E+ +VC + +C K +G C+ C +++ + IQ A +S Q +V + E C RLG + C + I Y + + + S K ICA + C + + + L+ + + ++ AK C++C F+V E+ L+ N +K I + + + +EC ++ Y S +++I+ E + P L C+ L LC+ T + V +++ C++C+K +L + KN T L C+ +C + ++ F C C + K +G ++C WGP +WC + + AA C AV+HC+ VWN
Sbjct: 56 PTVKSLPCDICKDVVTAAGDMLKDNATEEEILVYLE-KTCDWLPKPNMSASCKEIVDSYLPVILDIIKGEMSRPGEVCSALNLCESLQKHLAELNHQKQLESNKIPELDMTEVVAPFMANIPLLLYPQDGPRSKPQPKDNGDVCQDCIQMVTD-IQTAVRTNSTFVQALVEHVKEE--CDRLGPGMADICKNYISQYSEIAIQMMMHMSLQQPKEICALVGFCDEVKEMPMQTLVPAKVASKNVIPALELVEPIKKHEVPAKSDVYCEVCEFLVKEVTKLIDNNKTEKEILDAFDKMCSKLPKSLSEECQEVVDTYGSSILSILLEEVSPELVCSMLHLCSGTRLPALT----VHVTQPKDGGFCEVCKKLVGYLDRNLEKNSTKQEILAALEKGCSFLPDPYQKQCDQFVAEYEPVLIEILVEVMDPSFVCLKIGACPSAHKPLLGTEKCIWGPSYWCQNTETAAQC--NAVEHCKRHVWN 527 HSP 2 Score: 55.0694 bits (131), Expect = 5.929e-7 Identity = 88/440 (20.00%), Postives = 163/440 (37.05%), Query Frame = 2
Query: 581 CTKCKQVFQFLESMFSKVSPDEFKFLIESLICNQLP-SEVQSTCKLLLDGYVTQLYNLIKSLLD-GDRICPLIHLCSSENTQITLGSWQCQTCLDVIVVLRKDLAGVSAP--------------------------------EIVSLIKSNV-----------------CSKLNFFEKGICNSYVSNLVPTFVNEL-HSTLT-PQQICGNHHVCTS--------------------------HSIKKPIASEKGVIECDLCEIGVTGAKSFISKPENMNKVKSFIKS--KICSHL-KSISSQCSSLVDAYADQLITMISNHMDSKAICIAINACKGF---SISQRKSAPNFKGIECDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIFQSEKIQCQQLVDEFFDIILNFVLSELDVDKVCKVMGMC--SMKKVSGIE 1639
C CK V M + +E + C+ LP + ++CK ++D Y+ + ++IK + +C ++LC E+ Q L Q L+ + D+ V AP ++V+ I++ V C +L IC +Y+S + + H +L P++IC C IKK K + C++CE V I +N K + + K+CS L KS+S +C +VD Y +++++ + + +C ++ C G +++ + P G C+ C V + + + K I L+ + + QC Q V E+ +++ ++ +D VC +G C + K + G E
Sbjct: 63 CDICKDVVTAAGDMLKDNATEEEILVYLEKTCDWLPKPNMSASCKEIVDSYLPVILDIIKGEMSRPGEVCSALNLC--ESLQKHLAELNHQKQLESNKIPELDMTEVVAPFMANIPLLLYPQDGPRSKPQPKDNGDVCQDCIQMVTDIQTAVRTNSTFVQALVEHVKEECDRLGPGMADICKNYISQYSEIAIQMMMHMSLQQPKEICALVGFCDEVKEMPMQTLVPAKVASKNVIPALELVEPIKKHEVPAKSDVYCEVCEFLVKEVTKLI---DNNKTEKEILDAFDKMCSKLPKSLSEECQEVVDTYGSSILSILLEEVSPELVCSMLHLCSGTRLPALTVHVTQPKDGGF-CEVCKKLVGYLDRNLEKNSTKQEILAALEKGCSFLPDPYQKQCDQFVAEYEPVLIEILVEVMDPSFVCLKIGACPSAHKPLLGTE 496
BLAST of Proactivator polypeptide vs. Ensembl Human
Match: PSAPL1 (prosaposin like 1 (gene/pseudogene) [Source:HGNC Symbol;Acc:HGNC:33131]) HSP 1 Score: 73.559 bits (179), Expect = 9.807e-13 Identity = 113/530 (21.32%), Postives = 206/530 (38.87%), Query Frame = 2
Query: 1043 CGNHHVCTSHSIKKPIASEKGVIECDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHLKS--ISSQCSSLVDAYADQLITMISNHMDSKA--ICIAINACKGFSISQRKSAPNFKGIECDACHLAVTKIKEYISDPARKTSIKNFL---KMRICPIFQSEKIQCQQLVDEFFD----IILNFVLSELDVDKVCKVMGMCSMKKVSGIECEVCERVMDEVIQLAFSNHSQQVVSNFIENKICPRLGFVKTECTSLIDTYLPVIFSLARSTLNSKVICAELKLCSLISQKKINLLMKSNINDDSAKCKICHFVVSELLTLLTNPDIQKSIENFLENHVCPHFGAK-KQECINDINQYYSLVINIITEFLKPTLCNELRLCNSTTTSIIMSQ-----PLVKMSRAILSSNCQLCEKFY-FHLQKIFLKNTPISPLTVFANVCNLEDTDETNECLKHF--RNLNSIVAAFHASRNIGEFCKMTEMCKGSKKHHVGKKQCSWGPGFWCISRQHAAGCGPGAVKHCEMKVWNQM 2572
CG C KP A + CD+C+ A + ++ + + + + K C L S S+ C +VDA++ +++M+ DS +C A++ C+ QR HLA + +S ++ F+ + P E CQ V + + N L++L++ + C+ +G G+ +C+ + F + Q + ++C + GF + L L + V EL L +K+ + MK+ + C++C VV +L L + + I + LE VC A +ECI ++ Y ++ ++ + +C +RLC + + + P + S C C++ + K+T L F C++ +C KHF + ++ + + CK C G + +G QC+ GP FWC S++ A C AV+HC+ VW +M
Sbjct: 43 CGAVGYCQGAVWNKPTAKS---LPCDVCQDIAAAAGNGLNPDATESDILALVM-KTCEWLPSQESSAGCKWMVDAHSSAILSMLRGAPDSAPAQVCTALSLCEPL---QR--------------HLATLR---PLSKEDTFEAVAPFMANGPLTFHPRQAPEGALCQDCVRQVSRLQEAVRSNLTLADLNIQEQCESLG-------PGLAV-LCKNYL-----FQFFVPADQALRLLPPQELCRKGGFCEE---------LGAPARLTQVVAMDGVPSLELGL----PRKQSEMQMKAGVT-----CEVCMNVVQKLDHWLMSNSSELMITHALER-VCSVMPASITKECIILVDTYSPSLVQLVAKITPEKVCKFIRLCGNRRRARAVHDAYAIVPSPEWDAENQGSFCNGCKRLLTVSSHNLESKSTKRDILVAFKGGCSILPLPYMIQC-KHFVTQYEPVLIESLKDMMDPVAVCKKVGACHGPRTPLLGTDQCALGPSFWCRSQEAAKLC--NAVQHCQKHVWKEM 513
BLAST of Proactivator polypeptide vs. Ensembl Human
Match: PSAP (prosaposin [Source:HGNC Symbol;Acc:HGNC:9498]) HSP 1 Score: 51.2174 bits (121), Expect = 3.293e-6 Identity = 39/161 (24.22%), Postives = 66/161 (40.99%), Query Frame = 2
Query: 2090 INIITEFLKPTLCNELRLCNSTTTSIIMSQPLVKMSRAILSSNCQLCEKFYFHLQKIFLKN-TPISPLTVFANVCNLEDTDETNECLKHFRNLNSIVAAFHASRNIGEF-CKMTEMCKGSKKHHVGKKQCSWGPGFWCISRQHAAGCGPGAVKHCEMKVWN 2566
I + E +C+ L LC+ T + V +++ C++C+K +L + KN T L C+ +C + ++ F C C + K +G ++C WGP +WC + + AA C AV+HC+ VWN
Sbjct: 86 ILVYLEVSPELVCSMLHLCSGTRLPALT----VHVTQPKDGGFCEVCKKLVGYLDRNLEKNSTKQEILAALEKGCSFLPDPYQKQCDQFVAEYEPVLIEILVEVMDPSFVCLKIGACPSAHKPLLGTEKCIWGPSYWCQNTETAAQC--NAVEHCKRHVWN 240
BLAST of Proactivator polypeptide vs. Ensembl Celegans
Match: spp-10 (SaPosin-like Protein family [Source:UniProtKB/TrEMBL;Acc:Q4JFH6]) HSP 1 Score: 52.373 bits (124), Expect = 4.863e-7 Identity = 43/196 (21.94%), Postives = 83/196 (42.35%), Query Frame = 2
Query: 842 CQTCLDVIVVLRKDLAGVSAPEIVSLIKSNVCSKLNFFEKGICNSYVSNLVPTFVNELHSTL-TPQQICGNHHVCTSHSI------------KKPIASEKGVIECDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHLKSISSQCSSLVDAYADQLITMISNHM-DSKAICIAINACKGFSISQRKSAPNFKGI 1387
C+ C +VI + + E + ++ +C + ++ ++ C +VS L F+++L L P +C H+C++ I + + I CD C+ V K+ + ++ + + F++ +C L C +VD Y Q I + + D +C+ I AC S +Q A + GI
Sbjct: 38 CEECENVIKRISEAAKDPKKIEELKIVLGMMCRETSYADE--CRLFVSQL-DKFIDKLQPFLKNPHAVCTRLHICSNKKIDSFRRILLEFAKRAQKTFDAPAIVCDECQFVVKELKTVVEDKKSQAEARDFLRENVCKSLGQYRGFCDLVVDEYLPQFIQELDAILQDPHQVCVDIKACNSAS-AQGLKARKYVGI 229
BLAST of Proactivator polypeptide vs. Ensembl Celegans
Match: spp-10 (SaPosin-like Protein family [Source:UniProtKB/TrEMBL;Acc:Q4JFH6]) HSP 1 Score: 50.0618 bits (118), Expect = 6.739e-6 Identity = 40/189 (21.16%), Postives = 79/189 (41.80%), Query Frame = 2
Query: 842 CQTCLDVIVVLRKDLAGVSAPEIVSLIKSNVCSKLNFFEKGICNSYVSNLVPTFVNELHSTL-TPQQICGNHHVCTSHSI------------KKPIASEKGVIECDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHLKSISSQCSSLVDAYADQLITMISNHM-DSKAICIAINACKGFSISQRKS 1366
C+ C +VI + + E + ++ +C + ++ ++ C +VS L F+++L L P +C H+C++ I + + I CD C+ V K+ + ++ + + F++ +C L C +VD Y Q I + + D +C+ I AC S K+
Sbjct: 38 CEECENVIKRISEAAKDPKKIEELKIVLGMMCRETSYADE--CRLFVSQL-DKFIDKLQPFLKNPHAVCTRLHICSNKKIDSFRRILLEFAKRAQKTFDAPAIVCDECQFVVKELKTVVEDKKSQAEARDFLRENVCKSLGQYRGFCDLVVDEYLPQFIQELDAILQDPHQVCVDIKACNSASAQGLKA 223
BLAST of Proactivator polypeptide vs. Ensembl Fly
Match: Sap-r (gene:FBgn0000416 transcript:FBtr0085713) HSP 1 Score: 96.6709 bits (239), Expect = 5.539e-20 Identity = 128/589 (21.73%), Postives = 246/589 (41.77%), Query Frame = 2
Query: 557 IKFQNDDVCTKCKQ-VFQFLESMFSKVSPDEFKFLIESLICNQLP-SEVQSTCKLLLDGYVTQLYNLIKSLLDGDRICPLIHLC--------------------------SSENTQITLGSWQ--CQTCLDVIVVLRKDLAGVSAPEIVSLIKSNVCSKLNFFEKGICNSYVSNLVPTFVNELHST----LTPQQICGNHHVCTSH-----SIKKP----IASEKGV-IECDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHLKSISSQCSSLVDAYADQLITMISNHMDSKAICIAINACKGFSISQRKSAPNFKGIECDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIFQSEKIQCQQLVDEFFDIILNFVLSELDVDKVCKVMGMCSMKKVSGIE-CEVCERVMDEVIQLAFSNHSQQVVSNFIENKICPRL-GFVKTECTSLIDTYLPVIFSLARSTLNSKVICAELKLC--SLISQKKINLLMKSNIND--DSAKCKICHFVVSELLTLLTNPDIQKSIENFLENHVCPHFGAK-KQECINDINQYYSLVIN-IITEFLKPTLCNELRLCNSTTTSI 2167
+ D +CT CK V Q + + S + +E K + E C +P +Q C + D ++ +L + S ++ D++C + LC SSE T++ + Q C C + ++ A ++V + ++C L+ F + +N+V T+ N+++ LT +C VC S K+P +A + G I C+LCE V + + + K ++ C K +C S+VD Y + + + +D+ C I C+ S S K P + + I K +I+ K + SE QQ E D+ +L +D + ++ V G E C +CE ++ + + + + + + +EN IC +L V +C + ++ Y + +L LN + +C +++C +L ++ + + +D D C +C F V + + + + +I+ L N +C H + K+EC++ +N Y + +I+ +IT+F +C +L+LC TT ++
Sbjct: 64 VPVDTDSICTICKDMVTQARDQLKSNQTEEELKEVFEG-SCKLIPIKPIQKECIKVADDFLPELVEALASQMNPDQVCSVAGLCNSARIDELYKNGIQAGLDGTVQNEDDSSEETELAMQPNQLSCGNCNLLSRLMHSKFAATDRDDMVETM-LHMCGSLSSF-----SDACANIVLTYFNDIYDHVSKHLTTDAVCHVSGVCASRYHQHEEEKQPQEALVALDAGDDIPCELCEQLVKHLRDVLVANTTETEFKQVMEG-FCKQSKGFKDECLSIVDQYYHVIYETLVSKLDANGACCMIGICQKNSASSMKDVP-------------IMPLLPVIEPAQVKITIEKLEKHEKKQLGASEPKFSQQ---EILDM-------QLPIDHLMGAANPGAL--VEGGELCTLCEYMLHFIQETLATPSTDDEIKHTVEN-ICAKLPSGVAGQCRNFVEMYGDAVIALLVQGLNPRDVCPLMQMCPKNLPKKEDVEVFNPQPASDEQDPPTCPLCLFAVEQAQMKIRDNKSKDNIKKVL-NGLCSHLPNEIKEECVDFVNTYSNELIDMLITDFKPQEICVQLKLCPKTTYAL 617 HSP 2 Score: 94.7449 bits (234), Expect = 1.805e-19 Identity = 101/477 (21.17%), Postives = 189/477 (39.62%), Query Frame = 2
Query: 773 DRICPLIHLCSSENT-QITLGSWQCQTCLDVIVVLRKDLAGVSAPEIVSLIKSNVCSKLNFFEKGICNSYVSNLVPTFVNELHSTLTPQQICGNHHVCTSHSIKK---------PIASEKGVIECDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHLKS-ISSQCSSLVDAYADQLITMISNHMDSKAICIAINACKGFSIS--------------QRKSAP---NFKGIE--------------------CDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIFQSEKIQCQQLVDEFFDIILNFVLSELDVDKVCKVMGMCSMKKVSGIE----------------------------CEVCERVMDEVIQLAFSNHSQQVVSNFIENKICPRL-GFVKTECTSLIDTYLPVIFSLARSTLNSKVICAELKLCSLISQKKINLLMKSNINDDSAKCKICHFVVSELLTLL 1972
D P+ HL + N + G C C ++ +++ LA S + + N+C+KL G C ++V + L L P+ +C +C + KK P + E+ C LC V A+ I ++ + +K + +CSHL + I +C V+ Y+++LI M+ + IC+ + C + + + KS+ +F IE C C V +++ + + SIK+ L+ + + +C +++D++ D I + +L E+D +C +GMC + + +E C +CE +M ++ + Q + IE +C RL V+ +C + +D Y + L S + K +C +L+LC ++ + D+ +C +CH V LL L
Sbjct: 413 DMQLPIDHLMGAANPGALVEGGELCTLCEYMLHFIQETLATPSTDDEIKHTVENICAKLPSGVAGQCRNFVEMYGDAVIALLVQGLNPRDVCPLMQMCPKNLPKKEDVEVFNPQPASDEQDPPTCPLCLFAVEQAQMKIRDNKSKDNIKKVLNG-LCSHLPNEIKEECVDFVNTYSNELIDMLITDFKPQEICVQLKLCPKTTYALWDLRISLEDDVDGEDKSSSEEISFNDIESLEELPPQLAFDPGFTAAPNCLICEELVKTLEKRMGKHPTRDSIKHILEESCDRMRKPMNTKCHKVIDKYGDKIADLLLKEMDPKLICTELGMCILADLDDLEVDEALKYDVIALPRQDNKLSSSIKEPPTCVLCEFIMTKLDADLKNKTEQDDIKRAIE-AVCNRLPATVRKQCDTFVDGYASAVLKLL-SDVPPKQVCQKLQLCFSVA-----------VTDEVLECGVCHGVTQALLPFL 875 HSP 3 Score: 89.3521 bits (220), Expect = 1.023e-17 Identity = 159/848 (18.75%), Postives = 312/848 (36.79%), Query Frame = 2
Query: 158 CLKGPTYWCATYQNAEKCDRITECFMGQGD-QKNKVDISHHKNGDYCDACMKFVQSVEAHIPKISEQEFVNLAGNLICNNLKGKS-QLDCKKEVNFFAPLIYRELSMEATPEKICGLVQVCKNTNAMSFSKIF-NSIK------FQNDD---------------VCTKCKQVFQFLESMFSKVSPDEFKFLIESLI--CNQLPSEVQSTCKLLLDGYVTQLYNLIKSLLDGDRICPLIHLCSSENTQ-----------ITLGSWQ---CQTCLDVIVVLRKDLAGVSAPEIVSLIKSNVCSKLNFFEK---GICNSYVSNLVPTFVNELHS----------------------------TLTPQQICGNHHVCTSHSIKKPIASE----------------------------KGVIECDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHLKS-ISSQCSSLVDAYADQLITMISNHMDSKAICIAINACKGFSISQRKSAPNFKGIE---------------CDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIFQSE-KIQCQQLVDEFFDIILNFVLSELDVDKVCKVMGMC-----------------------------SMKKVSGIE-----------------CEVCERVMDEVIQLAFSNHSQQVVSNFIENKICPRL-GFVKTECTSLIDTYLPVIFSLARSTLNSKVICAELKLCSL-----------ISQKKINLLMKSNINDDSAK----CKICHFVVSELLTLLTNPDIQKSIENFLENHVCPHFGAK-KQECINDINQYYSLVINIITEFLKPTLCNELRLCNSTTTS 2164
C GP+YWC + N+++C C + QK VD C C V + +E + C + K Q +C K + F P + L+ + P+++C + +C N+ +++ N I+ QN+D C C + + + S F+ D+ ++E+++ C L S C ++ Y +Y+ + L D +C + +C+S Q + L + C+ C ++ LR L + + C + F+ I + Y + T V++L + + P Q+ H K+ ASE +G C LCE + + ++ P +++K +++ IC+ L S ++ QC + V+ Y D +I ++ ++ + +C + C K+ P + +E C C AV + + I D K +IK L +C +E K +C V+ + + +++ ++++ ++C + +C S + +E C +CE ++ + + + ++ + + +E C R+ + T+C +ID Y I L ++ K+IC EL +C L + I L + N S K C +C F++++L L N Q I+ +E VC A +++C ++ Y S V+ ++++ +C +L+LC S +
Sbjct: 31 CTWGPSYWCGNFSNSKECRATRHCIQTVWETQKVPVDTDS-----ICTICKDMVTQARDQLKSNQTEEELKEVFEGSCKLIPIKPIQKECIKVADDFLPELVEALASQMNPDQVCSVAGLC---NSARIDELYKNGIQAGLDGTVQNEDDSSEETELAMQPNQLSCGNCNLLSRLMHSKFAATDRDD---MVETMLHMCGSL-SSFSDACANIVLTYFNDIYDHVSKHLTTDAVCHVSGVCASRYHQHEEEKQPQEALVALDAGDDIPCELCEQLVKHLRDVLVANTTETEFKQVMEGFCKQSKGFKDECLSIVDQYYHVIYETLVSKLDANGACCMIGICQKNSASSMKDVPIMPLLPVIEPAQVKITIEKLEKHEKKQLGASEPKFSQQEILDMQLPIDHLMGAANPGALVEGGELCTLCEYMLHFIQETLATPSTDDEIKHTVEN-ICAKLPSGVAGQCRNFVEMYGDAVIALLVQGLNPRDVCPLMQMCP-------KNLPKKEDVEVFNPQPASDEQDPPTCPLCLFAVEQAQMKIRDNKSKDNIKKVLN-GLCSHLPNEIKEECVDFVNTYSNELIDMLITDFKPQEICVQLKLCPKTTYALWDLRISLEDDVDGEDKSSSEEISFNDIESLEELPPQLAFDPGFTAAPNCLICEELVKTLEKRMGKHPTRDSIKHILEES-CDRMRKPMNTKCHKVIDKYGDKIADLLLKEMDPKLICTELGMCILADLDDLEVDEALKYDVIALPRQDNKLSSSIKEPPTCVLCEFIMTKLDADLKNKTEQDDIKRAIE-AVCNRLPATVRKQCDTFVDGYASAVLKLLSDVPPKQVCQKLQLCFSVAVT 855 HSP 4 Score: 53.9138 bits (128), Expect = 8.187e-7 Identity = 87/499 (17.43%), Postives = 173/499 (34.67%), Query Frame = 2
Query: 278 KNGDYCDACMKFVQSVEAHIPKISEQEFVNLAGNLICNNLKGKSQLDCKKEVNFFAPLIYRELSMEATPEKICGLVQVC-KNTNAMSFSKIFNSIKFQNDDVCTKCKQVFQFLESMFSKV----SPDEFKFLIESLICNQLPSEVQSTCKLLLDGYVTQLYNLIKSLLDGDRICPLIHLCS----------------------SENTQITLGSWQ--------------------CQTCLDVIVVLRKDLAGVSAPEIVSLIKSNVCSKLNFFEKGICNSYVSNLVPTFVNELHSTLTPQQICGNHHVCT---------------------------SHSIKKPIASEKGVIECDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHLKS-ISSQCSSLVDAYADQLITMISNHMDSKAICIAINACKGFSISQRKSAPNFKGIECDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIFQSEKIQCQQLVDEFFDI 1549
+ G+ C C + ++ + S + + IC L C+ V + + L P +C L+Q+C KN ++FN ++ C +E K+ S D K ++ L C+ LP+E++ C ++ Y +L +++ + IC + LC S + +I+ + C C +++ L K + + + I C ++ C+ + + L + P+ IC +C S SIK+P C LCE +T + + + +K I++ +C+ L + + QC + VD YA ++ ++S+ + K +C + C FS++ +EC CH + ++ + S L+M + C+ L +++ I
Sbjct: 432 EGGELCTLCEYMLHFIQETLATPSTDDEIKHTVENICAKLPSGVAGQCRNFVEMYGDAVIALLVQGLNPRDVCPLMQMCPKNLPKKEDVEVFNPQPASDEQDPPTCPLCLFAVEQAQMKIRDNKSKDNIKKVLNGL-CSHLPNEIKEECVDFVNTYSNELIDMLITDFKPQEICVQLKLCPKTTYALWDLRISLEDDVDGEDKSSSEEISFNDIESLEELPPQLAFDPGFTAAPNCLICEELVKTLEKRMGKHPTRDSIKHILEESCDRMRKPMNTKCHKVIDKYGDKIADLLLKEMDPKLICTELGMCILADLDDLEVDEALKYDVIALPRQDNKLSSSIKEPPT-------CVLCEFIMTKLDADLKNKTEQDDIKRAIEA-VCNRLPATVRKQCDTFVDGYASAVLKLLSD-VPPKQVCQKLQLC--FSVAVTDEV-----LECGVCHGVTQALLPFLREKKDNVSEVTALQMT--------SVGCENLPAKYYKI 905
BLAST of Proactivator polypeptide vs. Ensembl Fly
Match: Sap-r (gene:FBgn0000416 transcript:FBtr0085714) HSP 1 Score: 94.7449 bits (234), Expect = 2.006e-19 Identity = 101/477 (21.17%), Postives = 189/477 (39.62%), Query Frame = 2
Query: 773 DRICPLIHLCSSENT-QITLGSWQCQTCLDVIVVLRKDLAGVSAPEIVSLIKSNVCSKLNFFEKGICNSYVSNLVPTFVNELHSTLTPQQICGNHHVCTSHSIKK---------PIASEKGVIECDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHLKS-ISSQCSSLVDAYADQLITMISNHMDSKAICIAINACKGFSIS--------------QRKSAP---NFKGIE--------------------CDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIFQSEKIQCQQLVDEFFDIILNFVLSELDVDKVCKVMGMCSMKKVSGIE----------------------------CEVCERVMDEVIQLAFSNHSQQVVSNFIENKICPRL-GFVKTECTSLIDTYLPVIFSLARSTLNSKVICAELKLCSLISQKKINLLMKSNINDDSAKCKICHFVVSELLTLL 1972
D P+ HL + N + G C C ++ +++ LA S + + N+C+KL G C ++V + L L P+ +C +C + KK P + E+ C LC V A+ I ++ + +K + +CSHL + I +C V+ Y+++LI M+ + IC+ + C + + + KS+ +F IE C C V +++ + + SIK+ L+ + + +C +++D++ D I + +L E+D +C +GMC + + +E C +CE +M ++ + Q + IE +C RL V+ +C + +D Y + L S + K +C +L+LC ++ + D+ +C +CH V LL L
Sbjct: 336 DMQLPIDHLMGAANPGALVEGGELCTLCEYMLHFIQETLATPSTDDEIKHTVENICAKLPSGVAGQCRNFVEMYGDAVIALLVQGLNPRDVCPLMQMCPKNLPKKEDVEVFNPQPASDEQDPPTCPLCLFAVEQAQMKIRDNKSKDNIKKVLNG-LCSHLPNEIKEECVDFVNTYSNELIDMLITDFKPQEICVQLKLCPKTTYALWDLRISLEDDVDGEDKSSSEEISFNDIESLEELPPQLAFDPGFTAAPNCLICEELVKTLEKRMGKHPTRDSIKHILEESCDRMRKPMNTKCHKVIDKYGDKIADLLLKEMDPKLICTELGMCILADLDDLEVDEALKYDVIALPRQDNKLSSSIKEPPTCVLCEFIMTKLDADLKNKTEQDDIKRAIE-AVCNRLPATVRKQCDTFVDGYASAVLKLL-SDVPPKQVCQKLQLCFSVA-----------VTDEVLECGVCHGVTQALLPFL 798 HSP 2 Score: 89.7373 bits (221), Expect = 7.528e-18 Identity = 124/543 (22.84%), Postives = 231/543 (42.54%), Query Frame = 2
Query: 629 KVSPDEFKFLIESLICNQLPSEVQSTCKLLLDGYVTQLY-NLIKSLLDG-----DRICPLIHLCSSENTQITLGSWQ--CQTCLDVIVVLRKDLAGVSAPEIVSLIKSNVCSKLNFFEKGICNSYVSNLVPTFVNELHST----LTPQQICGNHHVCTSH-----SIKKP----IASEKGV-IECDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHLKSISSQCSSLVDAYADQLITMISNHMDSKAICIAINACKGFSISQRKSAPNFKGIECDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIFQSEKIQCQQLVDEFFDIILNFVLSELDVDKVCKVMGMCSMKKVSGIE-CEVCERVMDEVIQLAFSNHSQQVVSNFIENKICPRL-GFVKTECTSLIDTYLPVIFSLARSTLNSKVICAELKLC--SLISQKKINLLMKSNIND--DSAKCKICHFVVSELLTLLTNPDIQKSIENFLENHVCPHFGAK-KQECINDINQYYSLVIN-IITEFLKPTLCNELRLCNSTTTSI 2167
KV+ D L+E+L P +V S L + +LY N I++ LDG D SSE T++ + Q C C + ++ A ++V + ++C L+ F + +N+V T+ N+++ LT +C VC S K+P +A + G I C+LCE V + + + K ++ C K +C S+VD Y + + + +D+ C I C+ S S K P + + I K +I+ K + SE QQ E D+ +L +D + ++ V G E C +CE ++ + + + + + + +EN IC +L V +C + ++ Y + +L LN + +C +++C +L ++ + + +D D C +C F V + + + + +I+ L N +C H + K+EC++ +N Y + +I+ +IT+F +C +L+LC TT ++
Sbjct: 40 KVADDFLPELVEALASQMNPDQVCSVAGLCNSARIDELYKNGIQAGLDGTVQNEDD--------SSEETELAMQPNQLSCGNCNLLSRLMHSKFAATDRDDMVETM-LHMCGSLSSF-----SDACANIVLTYFNDIYDHVSKHLTTDAVCHVSGVCASRYHQHEEEKQPQEALVALDAGDDIPCELCEQLVKHLRDVLVANTTETEFKQVMEG-FCKQSKGFKDECLSIVDQYYHVIYETLVSKLDANGACCMIGICQKNSASSMKDVP-------------IMPLLPVIEPAQVKITIEKLEKHEKKQLGASEPKFSQQ---EILDM-------QLPIDHLMGAANPGAL--VEGGELCTLCEYMLHFIQETLATPSTDDEIKHTVEN-ICAKLPSGVAGQCRNFVEMYGDAVIALLVQGLNPRDVCPLMQMCPKNLPKKEDVEVFNPQPASDEQDPPTCPLCLFAVEQAQMKIRDNKSKDNIKKVL-NGLCSHLPNEIKEECVDFVNTYSNELIDMLITDFKPQEICVQLKLCPKTTYAL 540 HSP 3 Score: 76.2554 bits (186), Expect = 1.026e-13 Identity = 131/657 (19.94%), Postives = 261/657 (39.73%), Query Frame = 2
Query: 452 IYRELSMEATPEKICGLVQVC-----KNTNAMSFSKIFNSIKFQNDDVCTKCKQVFQFLES-MFSKVSPDEFKFLIESLICNQLPSEVQSTCKLLLDGYVTQLYNLIKSLLDGDRICPLIHLCSSENTQITLGSWQCQTCLDVIVVLRKDLAGVSAPEIVSLIKSNVCSKLNFFEKGICNSYVSNLVPTFVNELHSTLTPQQICGNHHVCTSHSIKKPIASEKGVIECDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHLKS-ISSQCSSLVDAYADQLITMISNHMDSKAICIAINACKGFSISQRKSAPNFKGIE---------------CDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIFQSE-KIQCQQLVDEFFDIILNFVLSELDVDKVCKVMGMC-----------------------------SMKKVSGIE-----------------CEVCERVMDEVIQLAFSNHSQQVVSNFIENKICPRL-GFVKTECTSLIDTYLPVIFSLARSTLNSKVICAELKLCSL-----------ISQKKINLLMKSNINDDSAK----CKICHFVVSELLTLLTNPDIQKSIENFLENHVCPHFGAK-KQECINDINQYYSLVINIITEFLKPTLCNELRLCNSTTTS 2164
IY +S T + +C + VC ++ + ++ +D C C+Q+ + L + + + EFK ++E C Q + C ++D Y +Y + S LD + C +I +C +N+ ++ L VI P V + KL EK + P F + + Q+ +H + ++ P A +G C LCE + + ++ P +++K +++ IC+ L S ++ QC + V+ Y D +I ++ ++ + +C + C K+ P + +E C C AV + + I D K +IK L +C +E K +C V+ + + +++ ++++ ++C + +C S + +E C +CE ++ + + + ++ + + +E C R+ + T+C +ID Y I L ++ K+IC EL +C L + I L + N S K C +C F++++L L N Q I+ +E VC A +++C ++ Y S V+ ++++ +C +L+LC S +
Sbjct: 162 IYDHVSKHLTTDAVCHVSGVCASRYHQHEEEKQPQEALVALDAGDDIPCELCEQLVKHLRDVLVANTTETEFKQVMEGF-CKQSKG-FKDECLSIVDQYYHVIYETLVSKLDANGACCMIGICQ-KNSASSMKDVPIMPLLPVI-----------EPAQVKI----TIEKLEKHEK----KQLGASEPKFSQQ---EILDMQLPIDHLMGAAN----PGALVEGGELCTLCEYMLHFIQETLATPSTDDEIKHTVEN-ICAKLPSGVAGQCRNFVEMYGDAVIALLVQGLNPRDVCPLMQMCP-------KNLPKKEDVEVFNPQPASDEQDPPTCPLCLFAVEQAQMKIRDNKSKDNIKKVLN-GLCSHLPNEIKEECVDFVNTYSNELIDMLITDFKPQEICVQLKLCPKTTYALWDLRISLEDDVDGEDKSSSEEISFNDIESLEELPPQLAFDPGFTAAPNCLICEELVKTLEKRMGKHPTRDSIKHILEES-CDRMRKPMNTKCHKVIDKYGDKIADLLLKEMDPKLICTELGMCILADLDDLEVDEALKYDVIALPRQDNKLSSSIKEPPTCVLCEFIMTKLDADLKNKTEQDDIKRAIE-AVCNRLPATVRKQCDTFVDGYASAVLKLLSDVPPKQVCQKLQLCFSVAVT 778 HSP 4 Score: 59.6918 bits (143), Expect = 1.074e-8 Identity = 77/429 (17.95%), Postives = 163/429 (38.00%), Query Frame = 2
Query: 968 CNSYVSNLVPTFVNELHSTLTPQQICGNHHVCTSHSIKK------------------------PIASEKGVIECDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHLKSISSQCSSLVDAYADQLITMISNHMDSKAICIAINACKG-FSISQRKSAPN--------FKGIECDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIFQSEKIQCQQLVDEFFDIILNFVLSELDVDKVCKVMGMCSMKKVSGIECEVCERVMDEVIQLAFSNHSQQVVSNFIENKICPRLGFVKTECT--SLIDTYLPVIFSLARSTLNSKVICAELKLCSLISQKKINLLMKSNINDDSAKCKICHFVVSELLTLLTNPDIQKSIENFLENHVCPHFGAKKQECINDINQYYSLVINIITEFLKP-TLCNELRLC 2146
C + +P V L S + P Q+C +C S I + +A + + C C + S + + + V++ + +C L S S C+++V Y + + +S H+ + A+C C + + + P I C+ C V +++ + +T K ++ C + K +C +VD+++ +I ++S+LD + C ++G+C S ++ +V + VI+ A ++ +E +LG + + + ++D LP+ + + + V EL C +C +++ + L P I++ +EN +C N + Y VI ++ + L P +C +++C
Sbjct: 38 CIKVADDFLPELVEALASQMNPDQVCSVAGLCNSARIDELYKNGIQAGLDGTVQNEDDSSEETELAMQPNQLSCGNCNLLSRLMHSKFAATDRDDMVETMLH--MCGSLSSFSDACANIVLTYFNDIYDHVSKHLTTDAVCHVSGVCASRYHQHEEEKQPQEALVALDAGDDIPCELCEQLVKHLRDVLVANTTETEFKQVME-GFCKQSKGFKDECLSIVDQYYHVIYETLVSKLDANGACCMIGICQKNSASSMK-DVPIMPLLPVIEPA----QVKITIEKLEKHEKKQLGASEPKFSQQEILDMQLPIDHLMGAANPGALVEGGEL-------------------------CTLCEYMLHFIQETLATPSTDDEIKHTVENICAKLPSGVAGQCRNFVEMYGDAVIALLVQGLNPRDVCPLMQMC 433 HSP 5 Score: 52.7582 bits (125), Expect = 1.538e-6 Identity = 87/499 (17.43%), Postives = 173/499 (34.67%), Query Frame = 2
Query: 278 KNGDYCDACMKFVQSVEAHIPKISEQEFVNLAGNLICNNLKGKSQLDCKKEVNFFAPLIYRELSMEATPEKICGLVQVC-KNTNAMSFSKIFNSIKFQNDDVCTKCKQVFQFLESMFSKV----SPDEFKFLIESLICNQLPSEVQSTCKLLLDGYVTQLYNLIKSLLDGDRICPLIHLCS----------------------SENTQITLGSWQ--------------------CQTCLDVIVVLRKDLAGVSAPEIVSLIKSNVCSKLNFFEKGICNSYVSNLVPTFVNELHSTLTPQQICGNHHVCT---------------------------SHSIKKPIASEKGVIECDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHLK-SISSQCSSLVDAYADQLITMISNHMDSKAICIAINACKGFSISQRKSAPNFKGIECDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIFQSEKIQCQQLVDEFFDI 1549
+ G+ C C + ++ + S + + IC L C+ V + + L P +C L+Q+C KN ++FN ++ C +E K+ S D K ++ L C+ LP+E++ C ++ Y +L +++ + IC + LC S + +I+ + C C +++ L K + + + I C ++ C+ + + L + P+ IC +C S SIK+P C LCE +T + + + +K I++ +C+ L ++ QC + VD YA ++ ++S+ + K +C + C FS++ +EC CH + ++ + S L+M + C+ L +++ I
Sbjct: 355 EGGELCTLCEYMLHFIQETLATPSTDDEIKHTVENICAKLPSGVAGQCRNFVEMYGDAVIALLVQGLNPRDVCPLMQMCPKNLPKKEDVEVFNPQPASDEQDPPTCPLCLFAVEQAQMKIRDNKSKDNIKKVLNGL-CSHLPNEIKEECVDFVNTYSNELIDMLITDFKPQEICVQLKLCPKTTYALWDLRISLEDDVDGEDKSSSEEISFNDIESLEELPPQLAFDPGFTAAPNCLICEELVKTLEKRMGKHPTRDSIKHILEESCDRMRKPMNTKCHKVIDKYGDKIADLLLKEMDPKLICTELGMCILADLDDLEVDEALKYDVIALPRQDNKLSSSIKEPPT-------CVLCEFIMTKLDADLKNKTEQDDIKRAIEA-VCNRLPATVRKQCDTFVDGYASAVLKLLSD-VPPKQVCQKLQLC--FSVAVTDEV-----LECGVCHGVTQALLPFLREKKDNVSEVTALQMT--------SVGCENLPAKYYKI 828
BLAST of Proactivator polypeptide vs. Ensembl Zebrafish
Match: psap (prosaposin [Source:ZFIN;Acc:ZDB-GENE-020108-1]) HSP 1 Score: 87.8113 bits (216), Expect = 1.731e-17 Identity = 106/505 (20.99%), Postives = 203/505 (40.20%), Query Frame = 2
Query: 1292 NHMDSKAICIAINACKGFSISQRKSAPNFKGIECDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIFQSEKI--QCQQLVDEFFDIILNFVLSELD-VDKVCKVMGMCSMKKVSGIECEVCERVMDEV-------------IQL---------------------AFSNHSQ---QVVSNFIENKI------CPRLGFVKTE-CTSLIDTYLPVIFSLARSTLNSKVICAELKLC-------------------------SLISQKKINLLMKSNIND--DSAKCKICHFVVSELLTLLTNPDIQKSIENFLENHVCPHFGAK-KQECINDINQYYSLVINIITEFLKP-TLCNELRLCNSTTTSIIMSQPLVKMSRAILSSNCQLCEKFYFHLQKIFLKNTPISPLT-VFANVCN-LEDT--DETNECLKHFRNLNSIVAAFHASRNIGEFCKMTEMCKGSKKHHVGKKQCSWGPGFWCISRQHAAGCGPGAVKHCEMKVWN 2566
++ + ++C A+ C+ + P K + CD C + +++ + D ++ + +L+ + C + E + QC+++VD +F +++ + ELD VC +G+C ++ + + ++ + +V QL F N +Q +V S+FI I C LG ++ C I Y P++F S + K ICA C ++ +K + + N+ DS +C IC +V+ E+ ++ + + I +E VC + +C + I Y +I+++ + P T+C+ L LC+ + +M + C +C+ ++ I +N S + VC+ L D DE N+ ++ + L + + C C + + +G QCSWGP +WC + Q AA C A+ HC VW+
Sbjct: 32 QNVKTASLCGAVQHCQ----QNVWNKPQMKTVPCDLCKEVLVVVEQLLKDNVTESELLGYLE-KACQLIPDEGLANQCKEIVDNYFPVLMGIIQGELDDPGVVCGALGLCVSQQAALAKAQLTSNEIPQVDLNQRVSPFLLNIPQLLYPEEKRETPKQKGDVCQDCVTFINDTQDEARVNSSFISTLIAQVENQCELLGPGMSDMCKEYISQYGPLVFQQLMS-MQPKDICARAGFCPTKQKSVPMEKLLPAKSIPAVKMFPAVKVEKPVATMPAKNLVRVRDSPQCAICEYVMKEIENMIQDQTSEAEIVQAVEK-VCNILPSTLTAQCKDLIETYGQAIIDLLVQEADPKTVCSFLALCSGVSHVPVMDK-----QHFAAGGFCDVCKMAVRYVDGILEQNATQSEIEEAVLKVCSFLPDAVKDECNQLIEQYEPLLVQLLLQTLDPDF--VCMKLGACPEAVQRLLGLNQCSWGPAYWCKNVQTAARC--NALNHCRRHVWS 520 HSP 2 Score: 70.4774 bits (171), Expect = 4.752e-12 Identity = 101/465 (21.72%), Postives = 189/465 (40.65%), Query Frame = 2
Query: 491 ICGLVQVCKNTNAMSFSKIFNSIKFQNDDVCTKCKQVFQFLESMF-SKVSPDEFKFLIESLICNQLPSE-VQSTCKLLLDGYVTQLYNLIKSLLDGDRI-CPLIHLCSSENTQITLGSWQ-----------------------------------------CQTCLDVIVVLRKDLAGVSAPEIVSLIK--SNVCSKLNFFEKGICNSYVSNLVPTFVNELHSTLTPQQICGNHHVCTSHS------------------------IKKPIAS--EKGVI------ECDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHLKS-ISSQCSSLVDAYADQLITMISNHMDSKAICIAINACKGFSISQRKSAPNF-KGIECDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIF-QSEKIQCQQLVDEFFDIILNFVLSELDVDKVCKVMGMC--SMKKVSGI 1636
+CG VQ C+ ++N + + C CK+V +E + V+ E +E C +P E + + CK ++D Y L +I+ LD + C + LC S+ Q L Q CQ C+ I +D A V++ I +LI N C L +C Y+S P +L S + P+ IC C + ++KP+A+ K ++ +C +CE + ++ I + ++ ++ K+C+ L S +++QC L++ Y +I ++ D K +C + C G S +F G CD C +AV + + A ++ I+ + +++C + K +C QL++++ +++ +L LD D VC +G C +++++ G+
Sbjct: 39 LCGAVQHCQQN-------VWNKPQMKTVP-CDLCKEVLVVVEQLLKDNVTESELLGYLEK-ACQLIPDEGLANQCKEIVDNYFPVLMGIIQGELDDPGVVCGALGLCVSQ--QAALAKAQLTSNEIPQVDLNQRVSPFLLNIPQLLYPEEKRETPKQKGDVCQDCVTFIND-TQDEARVNSSFISTLIAQVENQCELLGPGMSDMCKEYISQYGPLVFQQLMS-MQPKDICARAGFCPTKQKSVPMEKLLPAKSIPAVKMFPAVKVEKPVATMPAKNLVRVRDSPQCAICEYVMKEIENMIQDQTSEAEIVQAVE-KVCNILPSTLTAQCKDLIETYGQAIIDLLVQEADPKTVCSFLALCSGVSHVPVMDKQHFAAGGFCDVCKMAVRYVDGILEQNATQSEIEEAV-LKVCSFLPDAVKDECNQLIEQYEPLLVQLLLQTLDPDFVCMKLGACPEAVQRLLGL 488
BLAST of Proactivator polypeptide vs. Ensembl Zebrafish
Match: psap (prosaposin [Source:ZFIN;Acc:ZDB-GENE-020108-1]) HSP 1 Score: 85.8853 bits (211), Expect = 6.517e-17 Identity = 106/506 (20.95%), Postives = 202/506 (39.92%), Query Frame = 2
Query: 1292 NHMDSKAICIAINACKGFSISQRKSAPNFKGIECDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIFQSEKI--QCQQLVDEFFDIILNFVLSELDVDKV-CKVMGMCSMKKVSGIECEVCERVMDEV-------------IQL---------------------AFSNHSQ---QVVSNFIENKI------CPRLGFVKTE-CTSLIDTYLPVIFS-LARSTLNSKVICAELKLC-------------------------SLISQKKINLLMKSNIND--DSAKCKICHFVVSELLTLLTNPDIQKSIENFLENHVCPHFGAK-KQECINDINQYYSLVINIITEFLKP-TLCNELRLCNSTTTSIIMSQPLVKMSRAILSSNCQLCEKFYFHLQKIFLKNTPISPLT-VFANVCN-LEDT--DETNECLKHFRNLNSIVAAFHASRNIGEFCKMTEMCKGSKKHHVGKKQCSWGPGFWCISRQHAAGCGPGAVKHCEMKVWN 2566
++ + ++C A+ C+ + P K + CD C + +++ + D ++ + +L+ + C + E + QC+++VD +F +++ + ELD V C +G+C ++ + + ++ + +V QL F N +Q +V S+FI I C LG ++ C I Y P++F L K ICA C ++ +K + + N+ DS +C IC +V+ E+ ++ + + I +E VC + +C + I Y +I+++ + P T+C+ L LC+ + +M + C +C+ ++ I +N S + VC+ L D DE N+ ++ + L + + C C + + +G QCSWGP +WC + Q AA C A+ HC VW+
Sbjct: 32 QNVKTASLCGAVQHCQ----QNVWNKPQMKTVPCDLCKEVLVVVEQLLKDNVTESELLGYLE-KACQLIPDEGLANQCKEIVDNYFPVLMGIIQGELDDPGVVCGALGLCVSQQAALAKAQLTSNEIPQVDLNQRVSPFLLNIPQLLYPEEKRETPKQKGDVCQDCVTFINDTQDEARVNSSFISTLIAQVENQCELLGPGMSDMCKEYISQYGPLVFQQLMSMEQQPKDICARAGFCPTKQKSVPMEKLLPAKSIPAVKMFPAVKVEKPVATMPAKNLVRVRDSPQCAICEYVMKEIENMIQDQTSEAEIVQAVEK-VCNILPSTLTAQCKDLIETYGQAIIDLLVQEADPKTVCSFLALCSGVSHVPVMDK-----QHFAAGGFCDVCKMAVRYVDGILEQNATQSEIEEAVLKVCSFLPDAVKDECNQLIEQYEPLLVQLLLQTLDPDF--VCMKLGACPEAVQRLLGLNQCSWGPAYWCKNVQTAARC--NALNHCRRHVWS 522 HSP 2 Score: 68.1662 bits (165), Expect = 2.480e-11 Identity = 101/466 (21.67%), Postives = 188/466 (40.34%), Query Frame = 2
Query: 491 ICGLVQVCKNTNAMSFSKIFNSIKFQNDDVCTKCKQVFQFLESMF-SKVSPDEFKFLIESLICNQLPSE-VQSTCKLLLDGYVTQLYNLIKSLLDGDRI-CPLIHLCSSENTQITLGSWQ-----------------------------------------CQTCLDVIVVLRKDLAGVSAPEIVSLIK--SNVCSKLNFFEKGICNSYVSNLVPTFVNELHST-LTPQQICGNHHVCTSHS------------------------IKKPIAS--EKGVI------ECDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHLKS-ISSQCSSLVDAYADQLITMISNHMDSKAICIAINACKGFSISQRKSAPNF-KGIECDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIF-QSEKIQCQQLVDEFFDIILNFVLSELDVDKVCKVMGMC--SMKKVSGI 1636
+CG VQ C+ ++N + + C CK+V +E + V+ E +E C +P E + + CK ++D Y L +I+ LD + C + LC S+ Q L Q CQ C+ I +D A V++ I +LI N C L +C Y+S P +L S P+ IC C + ++KP+A+ K ++ +C +CE + ++ I + ++ ++ K+C+ L S +++QC L++ Y +I ++ D K +C + C G S +F G CD C +AV + + A ++ I+ + +++C + K +C QL++++ +++ +L LD D VC +G C +++++ G+
Sbjct: 39 LCGAVQHCQQN-------VWNKPQMKTVP-CDLCKEVLVVVEQLLKDNVTESELLGYLEK-ACQLIPDEGLANQCKEIVDNYFPVLMGIIQGELDDPGVVCGALGLCVSQ--QAALAKAQLTSNEIPQVDLNQRVSPFLLNIPQLLYPEEKRETPKQKGDVCQDCVTFIND-TQDEARVNSSFISTLIAQVENQCELLGPGMSDMCKEYISQYGPLVFQQLMSMEQQPKDICARAGFCPTKQKSVPMEKLLPAKSIPAVKMFPAVKVEKPVATMPAKNLVRVRDSPQCAICEYVMKEIENMIQDQTSEAEIVQAVE-KVCNILPSTLTAQCKDLIETYGQAIIDLLVQEADPKTVCSFLALCSGVSHVPVMDKQHFAAGGFCDVCKMAVRYVDGILEQNATQSEIEEAV-LKVCSFLPDAVKDECNQLIEQYEPLLVQLLLQTLDPDFVCMKLGACPEAVQRLLGL 490 HSP 3 Score: 51.9878 bits (123), Expect = 3.279e-6 Identity = 50/214 (23.36%), Postives = 90/214 (42.06%), Query Frame = 2
Query: 269 SHHKNGDYCDACMKFVQSV--EAHIPKISEQEFVNLAGNLICNN--LKGKSQLD-CKKEVNFFAPLIYREL-SMEATPEKICGLVQVCKN------------TNAMSFSKIF---------------NSIKFQNDDVCTKCKQVFQFLESMF-SKVSPDEFKFLIESLICNQLPSEVQSTCKLLLDGYVTQLYNLIKSLLDGDRICPLIHLCSS 808
+ + GD C C+ F+ EA + F++ + N L G D CK+ ++ + PL++++L SME P+ IC C ++ K+F N ++ ++ C C+ V + +E+M + S E +E +CN LPS + + CK L++ Y + +L+ D +C + LCS
Sbjct: 181 TPKQKGDVCQDCVTFINDTQDEARV----NSSFISTLIAQVENQCELLGPGMSDMCKEYISQYGPLVFQQLMSMEQQPKDICARAGFCPTKQKSVPMEKLLPAKSIPAVKMFPAVKVEKPVATMPAKNLVRVRDSPQCAICEYVMKEIENMIQDQTSEAEIVQAVEK-VCNILPSTLTAQCKDLIETYGQAIIDLLVQEADPKTVCSFLALCSG 389
BLAST of Proactivator polypeptide vs. Ensembl Zebrafish
Match: sftpba (surfactant protein Ba [Source:ZFIN;Acc:ZDB-GENE-091204-378]) HSP 1 Score: 53.5286 bits (127), Expect = 3.631e-7 Identity = 63/261 (24.14%), Postives = 99/261 (37.93%), Query Frame = 2
Query: 1826 NSKVICAELKLCSLISQKKINLLMKSNIN----------DDSAKCKICHFVVSELLTLLTNPDIQKSIENFLENHVCPHFGAKKQ-ECINDINQYYSLVINIITEFLKPT-LCNELRLCNSTTTSIIMSQPLVKMSRAILSSNCQLCEKFYFHLQKIFLKN-TPISPLTVFANVCNLEDTDETNECLKHF-RNLNSIVAAFHASRNIGEFCKMTEMCKGSKKHHV-GKKQCSWGPGFWCISRQHAAGCGPGAVKHCEMKVW 2563
N IC L LC KI L+ ++++ + S +C +C ++VS L L Q I LE+ +C F Q +C + +Y ++++++ P +C LRLC + I + SNC C Q N T + +VC L + + +C + RN + + + CK +C K V CS GP + C Q A C GA C+ VW
Sbjct: 2 NPSEICTYLGLCGGRDSGKIKDLLLNHMSKTGTMPPMQMESSVQCSVCTYIVSTLGFLFPKERTQNIITVLLES-LCKEFPPLVQPQCNKLVGKYVQMLVDMLLNNTSPNFICTLLRLCEISERQI----------NTLALSNCDSCLTLAVLTQMHLGSNATEHQAASFLDSVCQL-NPNAMPKCETYIERNGKQLSLFLSKQQGALDTCKSANLCPSDDKESVITADPCSLGPKYSCRDLQTAVEC--GAFSFCQKSVW 248 HSP 2 Score: 51.2174 bits (121), Expect = 2.261e-6 Identity = 58/230 (25.22%), Postives = 97/230 (42.17%), Query Frame = 2
Query: 482 PEKICGLVQVCKNT----------NAMSFSKIFNSIKFQNDDVCTKCKQVFQFLESMFSK-VSPDEFKFLIESLICNQLPSEVQSTCKLLLDGYVTQLYNLIKSLLDGDRICPLIHLCSSENTQI-TLGSWQCQTCLDVIVVLRKDLAGVSAPEIVSLIKSNVCSKLNFFEKGICNSYVSNLVPTFVNELHSTLTPQQICGNHHVCTSHSIKKPIASEKGVIECDLCEIG 1135
P +IC + +C N MS + ++ ++ C+ C + L +F K + + L+ESL C + P VQ C L+ YV L +++ + + IC L+ LC QI TL C +CL + V+ + L + + +VC +LN C +Y+ +L L+ QQ G C S ++ P ++ VI D C +G
Sbjct: 3 PSEICTYLGLCGGRDSGKIKDLLLNHMSKTGTMPPMQMESSVQCSVCTYIVSTLGFLFPKERTQNIITVLLESL-CKEFPPLVQPQCNKLVGKYVQMLVDMLLNNTSPNFICTLLRLCEISERQINTLALSNCDSCLTLAVLTQMHLGSNATEHQAASFLDSVC-QLNPNAMPKCETYIER----NGKQLSLFLSKQQ--GALDTCKSANL-CPSDDKESVITADPCSLG 223
BLAST of Proactivator polypeptide vs. Ensembl Xenopus
Match: psap (prosaposin (variant Gaucher disease and variant metachromatic leukodystrophy) [Source:NCBI gene;Acc:550039]) HSP 1 Score: 83.5741 bits (205), Expect = 4.473e-16 Identity = 108/505 (21.39%), Postives = 204/505 (40.40%), Query Frame = 2
Query: 1106 VIECDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHLK--SISSQCSSLVDAYADQLITMISNHMDSKAI-CIAINACKGF-----SISQRKSAPNFKGIECDACHLAVTKIKEY--ISDPARKTSIKNFLKMRICPIFQSEKIQCQQLVDEFFDIIL-NFVLSELDVDKVCKVMGMCSMKKVSGIECEVCERVMDEVIQL---AFSNHSQQVVSNFIENKICPRLGFVKTECTSLIDTYLPVIFSLARSTLNSKVICAELKLCSLISQKKINLLMKSNINDDSAKCKICHFVVSELLTLLTNPDIQKSIENFLENHVCPHFGAK-KQECINDINQYYSLVINIITEFLKPT-LCNELRLCNSTTTSIIMSQPLVKMSRAILSSNCQLCEKFYFHLQKIFLKN-TPISPLTVFANVCNLEDTDETNECLKHFRNLNSI-VAAFHASRNIGEFCKMTEMCKGSKKHHVGKKQCSWGPGFWCISRQHAAGCGPGAVKHCEMKVWN 2566
+ CD+C+ +T +F+ ++K ++ +K+C L +S+ C + Y ++ M+ + + + C A+ C S+ K + + DA L I + +P +T K IC C +LV + D + N S+ VD ECE+ + M E+ + FS+ + QV+ ++C +GF E ++ + +P + + + V E L + C++C ++S++ LL N +++I++ LE VC A+ Q+C + I +YY +I ++ + P +C L C++ ++ + + +A C +C+ ++ ++ KN T I + VCN +EC + V + + C +C+ S+K +G ++C WGP +WC + AA C AV HC VWN
Sbjct: 2 ALPCDMCKEVITVVGNFLKDNMTQGEIKDYL-NKMCDLLPDPGLSATCKQELSEYFPIVLNMLEQELSNPGVLCSALGLCTSLQRHLASLKHPKQLLTNEIPDVDASKLVYPYIVNVPQLLNPQEET-FKEPKTGDICN-------DCLKLVSDVQDALRSNASFSKNLVDHALH-------------ECELLDPGMAEMCKSYINQFSDVAIQVLLQMQPKQLCSMVGFCDQEKSTPLQNIIPAKTLIPAAKVQPAVKITENPLAG-----------------NGVFCEVCQLMISQIEKLLDNNRTKENIKHSLEK-VCKLLPAEYSQKCEDIIEEYYDPLIELLEQEANPQFICTTLGYCSARRKNLQNVK--ISAEKAAAGDYCTVCKMMMKYVDELLEKNATEIRIKNFLSRVCNFLPDSMQDECSGLVNQYEPLFVQLLLEALDPSFICAKLHLCQ-SEKVLLGTEKCMWGPSYWCKDMETAANC--NAVDHCRRHVWN 461 HSP 2 Score: 83.5741 bits (205), Expect = 4.676e-16 Identity = 106/454 (23.35%), Postives = 199/454 (43.83%), Query Frame = 2
Query: 581 CTKCKQVFQFLESMF-SKVSPDEFKFLIESLICNQLPSE-VQSTCKLLLDGYVTQLYNLIKSLLDGDRI-CPLIHLCSSENTQITLGSWQCQTCLDVIVVLRKDLAGVSAPEIVSLIKSNVCSKLNFFEK--------GICNSYVSNLVPTFVNELHSTLTPQQICGNHHVCTSHSIKKPIASEKGVIECDLCEIGVTGA-KSFISKPENMNKVKSFIKSKICSHLKSISSQCSSLVDAYADQLITMISNHMDSKAICIAINACKGFSISQRKSAPNFKGIECDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIFQSEKIQ-CQQLVDEFFDIILNFVLSELDVDKVCKVMGMCSMKK-------------VSGIECEVCERVMDEVIQLAFSNHSQQVVSNFIENKICPRL-GFVKTECTSLIDTYLPVIFSLARSTLNSKVICAELKLC 1861
C CK+V + + ++ E K + + C+ LP + +TCK L Y + N+++ L + C + LC+S Q L S + L L ++ V A ++V NV LN E+ ICN + LV + L S + + +H + EC+L + G+ KS+I++ F I L+ Q S+V + T + N + +K + A I++ A N G+ C+ C L +++I++ + + K +IK+ L+ ++C + +E Q C+ +++E++D ++ + E + +C +G CS ++ +G C VC+ +M V +L N ++ + NF+ +++C L ++ EC+ L++ Y P+ L L+ ICA+L LC
Sbjct: 5 CDMCKEVITVVGNFLKDNMTQGEIKDYLNKM-CDLLPDPGLSATCKQELSEYFPIVLNMLEQELSNPGVLCSALGLCTS--LQRHLASLKHPKQL-----LTNEIPDVDASKLVYPYIVNVPQLLNPQEETFKEPKTGDICNDCLK-LVSDVQDALRSNASFSKNLVDH----------------ALHECELLDPGMAEMCKSYINQ---------FSDVAIQVLLQMQPKQLCSMVGFCDQEKSTPLQNIIPAKTLIPAAKVQPAVKITENPLAGN--GVFCEVCQLMISQIEKLLDNNRTKENIKHSLE-KVCKLLPAEYSQKCEDIIEEYYDPLIELLEQEANPQFICTTLGYCSARRKNLQNVKISAEKAAAGDYCTVCKMMMKYVDELLEKNATEIRIKNFL-SRVCNFLPDSMQDECSGLVNQYEPLFVQLLLEALDPSFICAKLHLC 420 HSP 3 Score: 65.0846 bits (157), Expect = 3.373e-10 Identity = 48/189 (25.40%), Postives = 85/189 (44.97%), Query Frame = 2
Query: 281 NGDYCDACMKFVQSVEAHIPKISEQEFVNLAGNLICNNLKGKSQLDCKKEVN-FFAPLIYRELSMEATPEKICGLVQVCKNTNAMSFSKIFNSIKFQNDDVCTKCKQVFQFLESMFSKVSPDEFKFLIESLICNQLPSEVQSTCKLLLDGYVTQLYNLIKSLLDGDRICPLIHLCSSENTQITLGSWQC 844
NG +C+ C + +E + +E + + +C L + C+ + ++ PLI L EA P+ IC + C + ++ K D CT CK + ++++ + K + + S +CN LP +Q C L++ Y L+ LD IC +HLC SE ++ LG+ +C
Sbjct: 247 NGVFCEVCQLMISQIEKLLDNNRTKENIKHSLEKVCKLLPAEYSQKCEDIIEEYYDPLI-ELLEQEANPQFICTTLGYCSARRKNLQNVKISAEKAAAGDYCTVCKMMMKYVDELLEKNATEIRIKNFLSRVCNFLPDSMQDECSGLVNQYEPLFVQLLLEALDPSFICAKLHLCQSE--KVLLGTEKC 432 HSP 4 Score: 53.9138 bits (128), Expect = 8.959e-7 Identity = 68/303 (22.44%), Postives = 126/303 (41.58%), Query Frame = 2
Query: 278 KNGDYCDACMKFVQSVEAHIPKISEQEFVNLAGNLICNNLKGKSQLD------CKKEVNFFAPLIYRELSMEATPEKICGLVQVCKNTNAMSF------------SKIFNSIKFQNDDV------CTKCKQVFQFLESMFSKVSPDE-FKFLIESLICNQLPSEVQSTCKLLLDGYVTQLYNLIKSLLDGDRICPLIHLCSS-----ENTQITL-----GSWQCQTCLDVIVVLRKDLAGVSAPEI-VSLIKSNVCSKLNFFEKGICNSYVSNLVPTFVNELHSTLTPQQICGNHHVCTSHSI 1078
K GD C+ C+K V V+ + + + + NL+ + L LD CK +N F+ + + L ++ P+++C +V C + +K+ ++K + + C C+ + +E + E K +E +C LP+E C+ +++ Y L L++ + IC + CS+ +N +I+ G + C C +++ +L +A EI + S VC+ L + C+ V+ P FV L L P IC H+C S +
Sbjct: 132 KTGDICNDCLKLVSDVQDAL-----RSNASFSKNLVDHALHECELLDPGMAEMCKSYINQFSDVAIQVL-LQMQPKQLCSMVGFCDQEKSTPLQNIIPAKTLIPAAKVQPAVKITENPLAGNGVFCEVCQLMISQIEKLLDNNRTKENIKHSLEK-VCKLLPAEYSQKCEDIIEEYYDPLIELLEQEANPQFICTTLGYCSARRKNLQNVKISAEKAAAGDY-CTVC-KMMMKYVDELLEKNATEIRIKNFLSRVCNFLPDSMQDECSGLVNQYEPLFVQLLLEALDPSFICAKLHLCQSEKV 425
BLAST of Proactivator polypeptide vs. Ensembl Xenopus
Match: psap (prosaposin (variant Gaucher disease and variant metachromatic leukodystrophy) [Source:NCBI gene;Acc:550039]) HSP 1 Score: 82.4185 bits (202), Expect = 1.129e-15 Identity = 106/454 (23.35%), Postives = 199/454 (43.83%), Query Frame = 2
Query: 581 CTKCKQVFQFLESMF-SKVSPDEFKFLIESLICNQLPSE-VQSTCKLLLDGYVTQLYNLIKSLLDGDRI-CPLIHLCSSENTQITLGSWQCQTCLDVIVVLRKDLAGVSAPEIVSLIKSNVCSKLNFFEK--------GICNSYVSNLVPTFVNELHSTLTPQQICGNHHVCTSHSIKKPIASEKGVIECDLCEIGVTGA-KSFISKPENMNKVKSFIKSKICSHLKSISSQCSSLVDAYADQLITMISNHMDSKAICIAINACKGFSISQRKSAPNFKGIECDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIFQSEKIQ-CQQLVDEFFDIILNFVLSELDVDKVCKVMGMCSMKK-------------VSGIECEVCERVMDEVIQLAFSNHSQQVVSNFIENKICPRL-GFVKTECTSLIDTYLPVIFSLARSTLNSKVICAELKLC 1861
C CK+V + + ++ E K + + C+ LP + +TCK L Y + N+++ L + C + LC+S Q L S + L L ++ V A ++V NV LN E+ ICN + LV + L S + + +H + EC+L + G+ KS+I++ F I L+ Q S+V + T + N + +K + A I++ A N G+ C+ C L +++I++ + + K +IK+ L+ ++C + +E Q C+ +++E++D ++ + E + +C +G CS ++ +G C VC+ +M V +L N ++ + NF+ +++C L ++ EC+ L++ Y P+ L L+ ICA+L LC
Sbjct: 29 CDMCKEVITVVGNFLKDNMTQGEIKDYLNKM-CDLLPDPGLSATCKQELSEYFPIVLNMLEQELSNPGVLCSALGLCTS--LQRHLASLKHPKQL-----LTNEIPDVDASKLVYPYIVNVPQLLNPQEETFKEPKTGDICNDCLK-LVSDVQDALRSNASFSKNLVDH----------------ALHECELLDPGMAEMCKSYINQ---------FSDVAIQVLLQMQPKQLCSMVGFCDQEKSTPLQNIIPAKTLIPAAKVQPAVKITENPLAGN--GVFCEVCQLMISQIEKLLDNNRTKENIKHSLE-KVCKLLPAEYSQKCEDIIEEYYDPLIELLEQEANPQFICTTLGYCSARRKNLQNVKISAEKAAAGDYCTVCKMMMKYVDELLEKNATEIRIKNFL-SRVCNFLPDSMQDECSGLVNQYEPLFVQLLLEALDPSFICAKLHLC 444 HSP 2 Score: 72.7886 bits (177), Expect = 1.303e-12 Identity = 102/498 (20.48%), Postives = 205/498 (41.16%), Query Frame = 2
Query: 1106 VIECDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHLK--SISSQCSSLVDAYADQLITMISNHMDSKAI-CIAINACKGF-----SISQRKSAPNFKGIECDACHLAVTKIKEY--ISDPARKTSIKNFLKMRICPIFQSEKIQCQQLVDEFFDIIL-NFVLSELDVDKVCKVMGMCSMKKVSGIECEVCERVMDEVIQL---AFSNHSQQVVSNFIENKICPRLGFVKTECTSLIDTYLPVIFSLARSTLNSKVICAELKLCSLISQKKINLLMKSNINDDSAKCKICHFVVSELLTLLTNPDIQKSIENFLENHVCPHFGAK-KQECINDINQYYSLVINIITEFLKPT-LCNELRLCNSTTTSIIMSQPLVKMSRAILSSNCQLCEKFYFHLQKIFLKN-TPISPLTVFANVCNLEDTDETNECLKHFRNLNSI-VAAFHASRNIGEFCKMTEMCKGSKKHHVGKKQCSWGPGFWCISRQHAAGCGPGAVKH 2545
+ CD+C+ +T +F+ ++K ++ +K+C L +S+ C + Y ++ M+ + + + C A+ C S+ K + + DA L I + +P +T K IC C +LV + D + N S+ VD ECE+ + M E+ + FS+ + QV+ ++C +GF E ++ + +P A++ + + + +K+ ++ + + C++C ++S++ LL N +++I++ LE VC A+ Q+C + I +YY +I ++ + P +C L C++ ++ + + +A C +C+ ++ ++ KN T I + VCN +EC + V + + C +C+ S+K +G ++C WGP +WC + AA C KH
Sbjct: 26 ALPCDMCKEVITVVGNFLKDNMTQGEIKDYL-NKMCDLLPDPGLSATCKQELSEYFPIVLNMLEQELSNPGVLCSALGLCTSLQRHLASLKHPKQLLTNEIPDVDASKLVYPYIVNVPQLLNPQEET-FKEPKTGDICN-------DCLKLVSDVQDALRSNASFSKNLVDHALH-------------ECELLDPGMAEMCKSYINQFSDVAIQVLLQMQPKQLCSMVGFCDQEKSTPLQNIIP-----AKTLIPAAKVQPAVKI------------TENPLAGNGVFCEVCQLMISQIEKLLDNNRTKENIKHSLEK-VCKLLPAEYSQKCEDIIEEYYDPLIELLEQEANPQFICTTLGYCSARRKNLQNVK--ISAEKAAAGDYCTVCKMMMKYVDELLEKNATEIRIKNFLSRVCNFLPDSMQDECSGLVNQYEPLFVQLLLEALDPSFICAKLHLCQ-SEKVLLGTEKCMWGPSYWCKDMETAANCNRYCSKH 480 HSP 3 Score: 64.6994 bits (156), Expect = 3.923e-10 Identity = 48/189 (25.40%), Postives = 85/189 (44.97%), Query Frame = 2
Query: 281 NGDYCDACMKFVQSVEAHIPKISEQEFVNLAGNLICNNLKGKSQLDCKKEVN-FFAPLIYRELSMEATPEKICGLVQVCKNTNAMSFSKIFNSIKFQNDDVCTKCKQVFQFLESMFSKVSPDEFKFLIESLICNQLPSEVQSTCKLLLDGYVTQLYNLIKSLLDGDRICPLIHLCSSENTQITLGSWQC 844
NG +C+ C + +E + +E + + +C L + C+ + ++ PLI L EA P+ IC + C + ++ K D CT CK + ++++ + K + + S +CN LP +Q C L++ Y L+ LD IC +HLC SE ++ LG+ +C
Sbjct: 271 NGVFCEVCQLMISQIEKLLDNNRTKENIKHSLEKVCKLLPAEYSQKCEDIIEEYYDPLI-ELLEQEANPQFICTTLGYCSARRKNLQNVKISAEKAAAGDYCTVCKMMMKYVDELLEKNATEIRIKNFLSRVCNFLPDSMQDECSGLVNQYEPLFVQLLLEALDPSFICAKLHLCQSE--KVLLGTEKC 456 HSP 4 Score: 53.5286 bits (127), Expect = 1.430e-6 Identity = 67/303 (22.11%), Postives = 128/303 (42.24%), Query Frame = 2
Query: 278 KNGDYCDACMKFVQSVEAHIPKISEQEFVNLAGNLICNNLKGKSQLD------CKKEVNFFAPLIYRELSMEATPEKICGLVQVCKNTNAMSF------------SKIFNSIKFQNDDV------CTKCKQVFQFLESMF-SKVSPDEFKFLIESLICNQLPSEVQSTCKLLLDGYVTQLYNLIKSLLDGDRICPLIHLCSS-----ENTQITL-----GSWQCQTCLDVIVVLRKDLAGVSAPEI-VSLIKSNVCSKLNFFEKGICNSYVSNLVPTFVNELHSTLTPQQICGNHHVCTSHSI 1078
K GD C+ C+K V V+ + + + + NL+ + L LD CK +N F+ + + L ++ P+++C +V C + +K+ ++K + + C C+ + +E + + + + K +E +C LP+E C+ +++ Y L L++ + IC + CS+ +N +I+ G + C C +++ +L +A EI + S VC+ L + C+ V+ P FV L L P IC H+C S +
Sbjct: 156 KTGDICNDCLKLVSDVQDAL-----RSNASFSKNLVDHALHECELLDPGMAEMCKSYINQFSDVAIQVL-LQMQPKQLCSMVGFCDQEKSTPLQNIIPAKTLIPAAKVQPAVKITENPLAGNGVFCEVCQLMISQIEKLLDNNRTKENIKHSLEK-VCKLLPAEYSQKCEDIIEEYYDPLIELLEQEANPQFICTTLGYCSARRKNLQNVKISAEKAAAGDY-CTVC-KMMMKYVDELLEKNATEIRIKNFLSRVCNFLPDSMQDECSGLVNQYEPLFVQLLLEALDPSFICAKLHLCQSEKV 449
BLAST of Proactivator polypeptide vs. Ensembl Xenopus
Match: psap (prosaposin (variant Gaucher disease and variant metachromatic leukodystrophy) [Source:NCBI gene;Acc:550039]) HSP 1 Score: 82.0333 bits (201), Expect = 1.757e-15 Identity = 106/454 (23.35%), Postives = 199/454 (43.83%), Query Frame = 2
Query: 581 CTKCKQVFQFLESMF-SKVSPDEFKFLIESLICNQLPSE-VQSTCKLLLDGYVTQLYNLIKSLLDGDRI-CPLIHLCSSENTQITLGSWQCQTCLDVIVVLRKDLAGVSAPEIVSLIKSNVCSKLNFFEK--------GICNSYVSNLVPTFVNELHSTLTPQQICGNHHVCTSHSIKKPIASEKGVIECDLCEIGVTGA-KSFISKPENMNKVKSFIKSKICSHLKSISSQCSSLVDAYADQLITMISNHMDSKAICIAINACKGFSISQRKSAPNFKGIECDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIFQSEKIQ-CQQLVDEFFDIILNFVLSELDVDKVCKVMGMCSMKK-------------VSGIECEVCERVMDEVIQLAFSNHSQQVVSNFIENKICPRL-GFVKTECTSLIDTYLPVIFSLARSTLNSKVICAELKLC 1861
C CK+V + + ++ E K + + C+ LP + +TCK L Y + N+++ L + C + LC+S Q L S + L L ++ V A ++V NV LN E+ ICN + LV + L S + + +H + EC+L + G+ KS+I++ F I L+ Q S+V + T + N + +K + A I++ A N G+ C+ C L +++I++ + + K +IK+ L+ ++C + +E Q C+ +++E++D ++ + E + +C +G CS ++ +G C VC+ +M V +L N ++ + NF+ +++C L ++ EC+ L++ Y P+ L L+ ICA+L LC
Sbjct: 8 CDMCKEVITVVGNFLKDNMTQGEIKDYLNKM-CDLLPDPGLSATCKQELSEYFPIVLNMLEQELSNPGVLCSALGLCTS--LQRHLASLKHPKQL-----LTNEIPDVDASKLVYPYIVNVPQLLNPQEETFKEPKTGDICNDCLK-LVSDVQDALRSNASFSKNLVDH----------------ALHECELLDPGMAEMCKSYINQ---------FSDVAIQVLLQMQPKQLCSMVGFCDQEKSTPLQNIIPAKTLIPAAKVQPAVKITENPLAGN--GVFCEVCQLMISQIEKLLDNNRTKENIKHSLE-KVCKLLPAEYSQKCEDIIEEYYDPLIELLEQEANPQFICTTLGYCSARRKNLQNVKISAEKAAAGDYCTVCKMMMKYVDELLEKNATEIRIKNFL-SRVCNFLPDSMQDECSGLVNQYEPLFVQLLLEALDPSFICAKLHLC 423 HSP 2 Score: 71.633 bits (174), Expect = 3.677e-12 Identity = 100/490 (20.41%), Postives = 203/490 (41.43%), Query Frame = 2
Query: 1109 IECDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHLK--SISSQCSSLVDAYADQLITMISNHMDSKAI-CIAINACKGF-----SISQRKSAPNFKGIECDACHLAVTKIKEY--ISDPARKTSIKNFLKMRICPIFQSEKIQCQQLVDEFFDIIL-NFVLSELDVDKVCKVMGMCSMKKVSGIECEVCERVMDEVIQL---AFSNHSQQVVSNFIENKICPRLGFVKTECTSLIDTYLPVIFSLARSTLNSKVICAELKLCSLISQKKINLLMKSNINDDSAKCKICHFVVSELLTLLTNPDIQKSIENFLENHVCPHFGAK-KQECINDINQYYSLVINIITEFLKPT-LCNELRLCNSTTTSIIMSQPLVKMSRAILSSNCQLCEKFYFHLQKIFLKN-TPISPLTVFANVCNLEDTDETNECLKHFRNLNSI-VAAFHASRNIGEFCKMTEMCKGSKKHHVGKKQCSWGPGFWCISRQHAAGC 2524
+ CD+C+ +T +F+ ++K ++ +K+C L +S+ C + Y ++ M+ + + + C A+ C S+ K + + DA L I + +P +T K IC C +LV + D + N S+ VD ECE+ + M E+ + FS+ + QV+ ++C +GF E ++ + +P A++ + + + +K+ ++ + + C++C ++S++ LL N +++I++ LE VC A+ Q+C + I +YY +I ++ + P +C L C++ ++ + + +A C +C+ ++ ++ KN T I + VCN +EC + V + + C +C+ S+K +G ++C WGP +WC + AA C
Sbjct: 6 LPCDMCKEVITVVGNFLKDNMTQGEIKDYL-NKMCDLLPDPGLSATCKQELSEYFPIVLNMLEQELSNPGVLCSALGLCTSLQRHLASLKHPKQLLTNEIPDVDASKLVYPYIVNVPQLLNPQEET-FKEPKTGDICN-------DCLKLVSDVQDALRSNASFSKNLVDHALH-------------ECELLDPGMAEMCKSYINQFSDVAIQVLLQMQPKQLCSMVGFCDQEKSTPLQNIIP-----AKTLIPAAKVQPAVKI------------TENPLAGNGVFCEVCQLMISQIEKLLDNNRTKENIKHSLEK-VCKLLPAEYSQKCEDIIEEYYDPLIELLEQEANPQFICTTLGYCSARRKNLQNVK--ISAEKAAAGDYCTVCKMMMKYVDELLEKNATEIRIKNFLSRVCNFLPDSMQDECSGLVNQYEPLFVQLLLEALDPSFICAKLHLCQ-SEKVLLGTEKCMWGPSYWCKDMETAANC 452 HSP 3 Score: 64.6994 bits (156), Expect = 4.165e-10 Identity = 48/189 (25.40%), Postives = 85/189 (44.97%), Query Frame = 2
Query: 281 NGDYCDACMKFVQSVEAHIPKISEQEFVNLAGNLICNNLKGKSQLDCKKEVN-FFAPLIYRELSMEATPEKICGLVQVCKNTNAMSFSKIFNSIKFQNDDVCTKCKQVFQFLESMFSKVSPDEFKFLIESLICNQLPSEVQSTCKLLLDGYVTQLYNLIKSLLDGDRICPLIHLCSSENTQITLGSWQC 844
NG +C+ C + +E + +E + + +C L + C+ + ++ PLI L EA P+ IC + C + ++ K D CT CK + ++++ + K + + S +CN LP +Q C L++ Y L+ LD IC +HLC SE ++ LG+ +C
Sbjct: 250 NGVFCEVCQLMISQIEKLLDNNRTKENIKHSLEKVCKLLPAEYSQKCEDIIEEYYDPLI-ELLEQEANPQFICTTLGYCSARRKNLQNVKISAEKAAAGDYCTVCKMMMKYVDELLEKNATEIRIKNFLSRVCNFLPDSMQDECSGLVNQYEPLFVQLLLEALDPSFICAKLHLCQSE--KVLLGTEKC 435 HSP 4 Score: 53.9138 bits (128), Expect = 1.050e-6 Identity = 68/303 (22.44%), Postives = 126/303 (41.58%), Query Frame = 2
Query: 278 KNGDYCDACMKFVQSVEAHIPKISEQEFVNLAGNLICNNLKGKSQLD------CKKEVNFFAPLIYRELSMEATPEKICGLVQVCKNTNAMSF------------SKIFNSIKFQNDDV------CTKCKQVFQFLESMFSKVSPDE-FKFLIESLICNQLPSEVQSTCKLLLDGYVTQLYNLIKSLLDGDRICPLIHLCSS-----ENTQITL-----GSWQCQTCLDVIVVLRKDLAGVSAPEI-VSLIKSNVCSKLNFFEKGICNSYVSNLVPTFVNELHSTLTPQQICGNHHVCTSHSI 1078
K GD C+ C+K V V+ + + + + NL+ + L LD CK +N F+ + + L ++ P+++C +V C + +K+ ++K + + C C+ + +E + E K +E +C LP+E C+ +++ Y L L++ + IC + CS+ +N +I+ G + C C +++ +L +A EI + S VC+ L + C+ V+ P FV L L P IC H+C S +
Sbjct: 135 KTGDICNDCLKLVSDVQDAL-----RSNASFSKNLVDHALHECELLDPGMAEMCKSYINQFSDVAIQVL-LQMQPKQLCSMVGFCDQEKSTPLQNIIPAKTLIPAAKVQPAVKITENPLAGNGVFCEVCQLMISQIEKLLDNNRTKENIKHSLEK-VCKLLPAEYSQKCEDIIEEYYDPLIELLEQEANPQFICTTLGYCSARRKNLQNVKISAEKAAAGDY-CTVC-KMMMKYVDELLEKNATEIRIKNFLSRVCNFLPDSMQDECSGLVNQYEPLFVQLLLEALDPSFICAKLHLCQSEKV 428
BLAST of Proactivator polypeptide vs. Ensembl Xenopus
Match: psap (prosaposin (variant Gaucher disease and variant metachromatic leukodystrophy) [Source:NCBI gene;Acc:550039]) HSP 1 Score: 77.7962 bits (190), Expect = 4.207e-14 Identity = 57/248 (22.98%), Postives = 116/248 (46.77%), Query Frame = 2
Query: 1226 SISSQCSSLVDAYADQLITMISNHMDSKAICIAINACKGFSISQRKSAP---------------------NFKGIECDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIFQSEKIQ-CQQLVDEFFDIILNFVLSELDVDKVCKVMGMCSMKK-------------VSGIECEVCERVMDEVIQLAFSNHSQQVVSNFIENKICPRL-GFVKTECTSLIDTYLPVIFSLARSTLNSKVICAELKLC 1861
++ C S ++ ++D I ++ M K +C + C Q KS P G+ C+ C L +++I++ + + K +IK+ L+ ++C + +E Q C+ +++E++D ++ + E + +C +G CS ++ +G C VC+ +M V +L N ++ + NF+ +++C L ++ EC+ L++ Y P+ L L+ ICA+L LC
Sbjct: 196 GMAEMCKSYINQFSDVAIQVLL-QMQPKQLCSMVGFC-----DQEKSTPLQNIIPAKTLIPAAKITENPLAGNGVFCEVCQLMISQIEKLLDNNRTKENIKHSLE-KVCKLLPAEYSQKCEDIIEEYYDPLIELLEQEANPQFICTTLGYCSARRKNLQNVKISAEKAAAGDYCTVCKMMMKYVDELLEKNATEIRIKNFL-SRVCNFLPDSMQDECSGLVNQYEPLFVQLLLEALDPSFICAKLHLC 435 HSP 2 Score: 71.2478 bits (173), Expect = 4.775e-12 Identity = 105/494 (21.26%), Postives = 202/494 (40.89%), Query Frame = 2
Query: 1097 EKGVIECDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHLK--SISSQCSSLVDAYADQLITMISNHMDSKAI-CIAINACKGF-----SISQRKSAPNFKGIECDACHLAVTKIKEY--ISDPARKTSIKNFLKMRICPIFQSEKIQCQQLVDEFFDIIL-NFVLSELDVDKVCKVMGMCSMKKVSGIECEVCERVMDEVIQL---AFSNHSQQVVSNFIENKICPRLGFVKTECTSLIDTYLPVIFSLARSTLNSKVICAELKLCSLISQKKINLLMKSNINDDSAKCKICHFVVSELLTLLTNPDIQKSIENFLENHVCPHFGAK-KQECINDINQYYSLVINIITEFLKPT-LCNELRLCNSTTTSIIMSQPLVKMSRAILSSNCQLCEKFYFHLQKIFLKN-TPISPLTVFANVCNLEDTDETNECLKHFRNLNSI-VAAFHASRNIGEFCKMTEMCKGSKKHHVGKKQCSWGPGFWCISRQHAAGC 2524
+ + CD+C+ +T +F+ ++K ++ +K+C L +S+ C + Y ++ M+ + + + C A+ C S+ K + + DA L I + +P +T K IC C +LV + D + N S+ VD ECE+ + M E+ + FS+ + QV+ ++C +GF E +ST +I A+ +LI KI ++ + + C++C ++S++ LL N +++I++ LE VC A+ Q+C + I +YY +I ++ + P +C L C++ ++ + + +A C +C+ ++ ++ KN T I + VCN +EC + V + + C +C+ S+K +G ++C WGP +WC + AA C
Sbjct: 20 RQKALPCDMCKEVITVVGNFLKDNMTQGEIKDYL-NKMCDLLPDPGLSATCKQELSEYFPIVLNMLEQELSNPGVLCSALGLCTSLQRHLASLKHPKQLLTNEIPDVDASKLVYPYIVNVPQLLNPQEET-FKEPKTGDICN-------DCLKLVSDVQDALRSNASFSKNLVDHALH-------------ECELLDPGMAEMCKSYINQFSDVAIQVLLQMQPKQLCSMVGFCDQE----------------KSTPLQNIIPAK----TLIPAAKIT---ENPLAGNGVFCEVCQLMISQIEKLLDNNRTKENIKHSLEK-VCKLLPAEYSQKCEDIIEEYYDPLIELLEQEANPQFICTTLGYCSARRKNLQNVK--ISAEKAAAGDYCTVCKMMMKYVDELLEKNATEIRIKNFLSRVCNFLPDSMQDECSGLVNQYEPLFVQLLLEALDPSFICAKLHLCQ-SEKVLLGTEKCMWGPSYWCKDMETAANC 464 HSP 3 Score: 64.6994 bits (156), Expect = 4.561e-10 Identity = 48/189 (25.40%), Postives = 85/189 (44.97%), Query Frame = 2
Query: 281 NGDYCDACMKFVQSVEAHIPKISEQEFVNLAGNLICNNLKGKSQLDCKKEVN-FFAPLIYRELSMEATPEKICGLVQVCKNTNAMSFSKIFNSIKFQNDDVCTKCKQVFQFLESMFSKVSPDEFKFLIESLICNQLPSEVQSTCKLLLDGYVTQLYNLIKSLLDGDRICPLIHLCSSENTQITLGSWQC 844
NG +C+ C + +E + +E + + +C L + C+ + ++ PLI L EA P+ IC + C + ++ K D CT CK + ++++ + K + + S +CN LP +Q C L++ Y L+ LD IC +HLC SE ++ LG+ +C
Sbjct: 262 NGVFCEVCQLMISQIEKLLDNNRTKENIKHSLEKVCKLLPAEYSQKCEDIIEEYYDPLI-ELLEQEANPQFICTTLGYCSARRKNLQNVKISAEKAAAGDYCTVCKMMMKYVDELLEKNATEIRIKNFLSRVCNFLPDSMQDECSGLVNQYEPLFVQLLLEALDPSFICAKLHLCQSE--KVLLGTEKC 447 HSP 4 Score: 55.8398 bits (133), Expect = 3.010e-7 Identity = 69/297 (23.23%), Postives = 123/297 (41.41%), Query Frame = 2
Query: 278 KNGDYCDACMKFVQSVEAHIPKISEQEFVNLAGNLICNNLKGKSQLD------CKKEVNFFAPLIYRELSMEATPEKICGLVQVCKNTNAMSF------------SKIFNSIKFQNDDVCTKCKQVFQFLESMFSKVSPDE-FKFLIESLICNQLPSEVQSTCKLLLDGYVTQLYNLIKSLLDGDRICPLIHLCSS-----ENTQITL-----GSWQCQTCLDVIVVLRKDLAGVSAPEI-VSLIKSNVCSKLNFFEKGICNSYVSNLVPTFVNELHSTLTPQQICGNHHVCTSHSI 1078
K GD C+ C+K V V+ + + + + NL+ + L LD CK +N F+ + + L ++ P+++C +V C + +KI + N C C+ + +E + E K +E +C LP+E C+ +++ Y L L++ + IC + CS+ +N +I+ G + C C +++ +L +A EI + S VC+ L + C+ V+ P FV L L P IC H+C S +
Sbjct: 153 KTGDICNDCLKLVSDVQDAL-----RSNASFSKNLVDHALHECELLDPGMAEMCKSYINQFSDVAIQVL-LQMQPKQLCSMVGFCDQEKSTPLQNIIPAKTLIPAAKITENPLAGNGVFCEVCQLMISQIEKLLDNNRTKENIKHSLEK-VCKLLPAEYSQKCEDIIEEYYDPLIELLEQEANPQFICTTLGYCSARRKNLQNVKISAEKAAAGDY-CTVC-KMMMKYVDELLEKNATEIRIKNFLSRVCNFLPDSMQDECSGLVNQYEPLFVQLLLEALDPSFICAKLHLCQSEKV 440
BLAST of Proactivator polypeptide vs. Ensembl Xenopus
Match: psap (prosaposin (variant Gaucher disease and variant metachromatic leukodystrophy) [Source:NCBI gene;Acc:550039]) HSP 1 Score: 77.0258 bits (188), Expect = 6.246e-14 Identity = 112/505 (22.18%), Postives = 207/505 (40.99%), Query Frame = 2
Query: 1106 VIECDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHLK--SISSQCSSLVDAYADQLITMISNHMDSKAI-CIAINACKGF-----SISQRKSAPNFKGIECDACHLAVTKIKEY--ISDPARKTSIKNFLKMRICPIFQSEKIQCQQLVDEFFDIIL-NFVLSELDVDKVCKVMGMCSMKKVSGIECEVCERVMDEVIQL---AFSNHSQQVVSNFIENKICPRLGFVKTECTSLIDTYLPVIFSLARSTLNSKVICAELKLCSLISQKKINLLMKSNINDDSAKCKICHFVVSELLTLLTNPDIQKSIENFLENHVCPHFGAK-KQECINDINQYYSLVINIITEFLKPT-LCNELRLC-----NSTTTSIIMSQPLVKMS--RAILSSNCQLCEKFYFHLQKIFLKN-TPISPLTVFANVCNLEDTDETNECLKHFRNLNSI-VAAFHASRNIGEFCKMTEMCKGSKKHHVGKKQCSWGPGFWCISRQHAAGCGPGAVKH 2545
+ CD+C+ +T +F+ ++K ++ +K+C L +S+ C + Y ++ M+ + + + C A+ C S+ K + + DA L I + +P +T K IC C +LV + D + N S+ VD ECE+ + M E+ + FS+ + QV+ ++C +GF E +ST +I A+ +LI KI ++ + + C++C ++S++ LL N +++I++ LE VC A+ Q+C + I +YY +I ++ + P +C L C N +++ PL+K+S +A C +C+ ++ ++ KN T I + VCN +EC + V + + C +C+ S+K +G ++C WGP +WC + AA C KH
Sbjct: 24 ALPCDMCKEVITVVGNFLKDNMTQGEIKDYL-NKMCDLLPDPGLSATCKQELSEYFPIVLNMLEQELSNPGVLCSALGLCTSLQRHLASLKHPKQLLTNEIPDVDASKLVYPYIVNVPQLLNPQEET-FKEPKTGDICN-------DCLKLVSDVQDALRSNASFSKNLVDHALH-------------ECELLDPGMAEMCKSYINQFSDVAIQVLLQMQPKQLCSMVGFCDQE----------------KSTPLQNIIPAK----TLIPAAKIT---ENPLAGNGVFCEVCQLMISQIEKLLDNNRTKENIKHSLEK-VCKLLPAEYSQKCEDIIEEYYDPLIELLEQEANPQFICTTLGYCSARRKNLQNGNMLRGIPLLKISAEKAAAGDYCTVCKMMMKYVDELLEKNATEIRIKNFLSRVCNFLPDSMQDECSGLVNQYEPLFVQLLLEALDPSFICAKLHLCQ-SEKVLLGTEKCMWGPSYWCKDMETAANCNRYCSKH 481 HSP 2 Score: 74.7146 bits (182), Expect = 2.836e-13 Identity = 109/477 (22.85%), Postives = 208/477 (43.61%), Query Frame = 2
Query: 539 SKIFNSIKFQNDDVCTKCKQVFQFLESMF-SKVSPDEFKFLIESLICNQLPSE-VQSTCKLLLDGYVTQLYNLIKSLLDGDRI-CPLIHLCSSENTQITLGSWQCQTCLDVIVVLRKDLAGVSAPEIVSLIKSNVCSKLNFFEK--------GICNSYVSNLVPTFVNELHSTLTPQQICGNHHVCTSHSIKKPIASEKGVIECDLCEIGVTGA-KSFISKPENMNKVKSFIKSKICSHLKSISSQCSSLVDAYADQLITMISNHMDSKAICIAINACKGFSISQRKSAPNFKGIECDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIFQSEKIQ-CQQLVDEFFDIILNFVLSELDVDKVCKVMGMCSMKK----------------------VSGIECEVCERVMDEVIQLAFSNHSQQVVSNFIENKICPRL-GFVKTECTSLIDTYLPVIFSLARSTLNSKVICAELKLC 1861
++ FN + C CK+V + + ++ E K + + C+ LP + +TCK L Y + N+++ L + C + LC+S Q L S + L L ++ V A ++V NV LN E+ ICN + V+++ L ++ S K + + + EC+L + G+ KS+I++ F I L+ Q S+V + T + N + +K + I A K I++ A N G+ C+ C L +++I++ + + K +IK+ L+ ++C + +E Q C+ +++E++D ++ + E + +C +G CS ++ +G C VC+ +M V +L N ++ + NF+ +++C L ++ EC+ L++ Y P+ L L+ ICA+L LC
Sbjct: 13 TRDFNPHQGPKALPCDMCKEVITVVGNFLKDNMTQGEIKDYLNKM-CDLLPDPGLSATCKQELSEYFPIVLNMLEQELSNPGVLCSALGLCTS--LQRHLASLKHPKQL-----LTNEIPDVDASKLVYPYIVNVPQLLNPQEETFKEPKTGDICNDCLK-----LVSDVQDALR-----------SNASFSKNLV-DHALHECELLDPGMAEMCKSYINQ---------FSDVAIQVLLQMQPKQLCSMVGFCDQEKSTPLQNIIPAKTL---IPAAK---ITENPLAGN--GVFCEVCQLMISQIEKLLDNNRTKENIKHSLE-KVCKLLPAEYSQKCEDIIEEYYDPLIELLEQEANPQFICTTLGYCSARRKNLQNGNMLRGIPLLKISAEKAAAGDYCTVCKMMMKYVDELLEKNATEIRIKNFL-SRVCNFLPDSMQDECSGLVNQYEPLFVQLLLEALDPSFICAKLHLC 445 HSP 3 Score: 63.1586 bits (152), Expect = 1.521e-9 Identity = 50/198 (25.25%), Postives = 89/198 (44.95%), Query Frame = 2
Query: 281 NGDYCDACMKFVQSVEAHIPKISEQEFVNLAGNLICNNLKGKSQLDCKKEVN-FFAPLIYRELSMEATPEKICGLVQVC-------KNTNAMSFSKIF--NSIKFQNDDVCTKCKQVFQFLESMFSKVSPDEFKFLIESLICNQLPSEVQSTCKLLLDGYVTQLYNLIKSLLDGDRICPLIHLCSSENTQITLGSWQC 844
NG +C+ C + +E + +E + + +C L + C+ + ++ PLI L EA P+ IC + C +N N + + ++ K D CT CK + ++++ + K + + S +CN LP +Q C L++ Y L+ LD IC +HLC SE ++ LG+ +C
Sbjct: 263 NGVFCEVCQLMISQIEKLLDNNRTKENIKHSLEKVCKLLPAEYSQKCEDIIEEYYDPLI-ELLEQEANPQFICTTLGYCSARRKNLQNGNMLRGIPLLKISAEKAAAGDYCTVCKMMMKYVDELLEKNATEIRIKNFLSRVCNFLPDSMQDECSGLVNQYEPLFVQLLLEALDPSFICAKLHLCQSE--KVLLGTEKC 457 HSP 4 Score: 53.1434 bits (126), Expect = 1.792e-6 Identity = 67/305 (21.97%), Postives = 119/305 (39.02%), Query Frame = 2
Query: 278 KNGDYCDACMKFVQSVEAHIPKISEQEFVNLAGNLICNNLKGKSQLD------CKKEVNFFAPLIYRELSMEATPEKICGLVQVCKNTNAMSF------------SKIFNSIKFQNDDVCTKCKQVFQFLESMFSKVSPDE-FKFLIESLICNQLPSEVQSTCKLLLDGYVTQLYNLIKSLLDGDRICPLIHLCSSENTQITLGSW------------------QCQTCLDVIVVLRKDLAGVSAPEI-VSLIKSNVCSKLNFFEKGICNSYVSNLVPTFVNELHSTLTPQQICGNHHVCTSHSI 1078
K GD C+ C+K V V+ + + + + NL+ + L LD CK +N F+ + + L ++ P+++C +V C + +KI + N C C+ + +E + E K +E +C LP+E C+ +++ Y L L++ + IC + CS+ + G+ C C +++ +L +A EI + S VC+ L + C+ V+ P FV L L P IC H+C S +
Sbjct: 154 KTGDICNDCLKLVSDVQDAL-----RSNASFSKNLVDHALHECELLDPGMAEMCKSYINQFSDVAIQVL-LQMQPKQLCSMVGFCDQEKSTPLQNIIPAKTLIPAAKITENPLAGNGVFCEVCQLMISQIEKLLDNNRTKENIKHSLEK-VCKLLPAEYSQKCEDIIEEYYDPLIELLEQEANPQFICTTLGYCSARRKNLQNGNMLRGIPLLKISAEKAAAGDYCTVC-KMMMKYVDELLEKNATEIRIKNFLSRVCNFLPDSMQDECSGLVNQYEPLFVQLLLEALDPSFICAKLHLCQSEKV 450
BLAST of Proactivator polypeptide vs. Ensembl Mouse
Match: Psapl1 (prosaposin-like 1 [Source:MGI Symbol;Acc:MGI:1924193]) HSP 1 Score: 73.1738 bits (178), Expect = 7.105e-13 Identity = 108/523 (20.65%), Postives = 197/523 (37.67%), Query Frame = 2
Query: 1061 CTSHSIKKPIASEKGVIECDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHLKS--ISSQCSSLVDAYADQLITMISN--HMDSKAICIAINACKGFSISQRKSAPNFKGIECDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIFQSEKIQ------CQQLVDEFFDII-LNFVLSELDVDKVCKVMGMCSMKKVSGIECEVCERVMDEVIQLAFSNHSQQVVSNFIENKICPRLGFVKTECTSLIDTYLPVIFSLARSTLNSKVICAELKLCSLISQKKINLLMKSNINDDSAKCKICHFVVSELLTLLTNPDIQKSIENFLEN--HVCPHFGAKKQECINDINQYYSLVINIITEFLKPTLCNELRLCNS-----TTTSIIMSQPLVKMSRAILSSNCQLCEKFY-FHLQKIFLKNTPISPLTVFANVCNLEDTDETNECLKHFRNLNSI-VAAFHASRNIGEFCKMTEMCKGSKKHHVGKKQCSWGPGFWCISRQHAAGCGPGAVKHCEMKVWNQ 2569
C S KP + C +C+ A + ++ + + + + K C L S S++C +V+ ++ +++M+S D ++C A+ C+ QR HLA T + ++ + FL S+ + C QL+ D + N L+E+ V C+ MG G+ +CE + A +Q + ++C + GF + E L R V E+++ + L M+ + C +C +V EL L + I + LE V P Q+CI ++ Y ++ ++++ +C ++LC S + + + + P + + S CQ C++ Q + K+T L F C + +C + + + + N + CK C G K +G QC GP FWC S + A C A++HC+ VW +
Sbjct: 52 CQSAVWNKPTVKS---LPCSVCQDVAAAAGNGVNPGATESDILTSVM-KTCEWLPSQESSAKCKWMVNNHSAAVLSMLSGAQETDLASVCTALTLCEPL---QR--------------HLAETTSERPLTQEDANEVMAPFLSNGALSFHPSQMPEGAVCHDCVQLISLLQDALESNLTLAEVTVQNQCQSMG-------PGLA-ALCENYIHRQFVPA-----KQTLQGLPPQEVCRKGGFCERESAHW----------LTRVAAVDGVPSLEMEM-----PRTNELQMQLGLT-----CDVCLNLVQELDKWLVTNSTEALISHTLERVCTVVPE--PLVQQCITLVDTYSPELVQLMSKVTPEKVCETIKLCGSKRRARSISRAVATTPSLPVDEENQGSFCQGCKRLLGMSSQNLDHKSTKRDILNAFKGGCRILPLPYVMQCNRFVAEYEPVLIESLKFMMNPTDLCKKMGACHGPKTPLLGTDQCVMGPSFWCKSPEAAEMC--NALEHCQRLVWKK 516
BLAST of Proactivator polypeptide vs. Ensembl Mouse
Match: Psap (prosaposin [Source:MGI Symbol;Acc:MGI:97783]) HSP 1 Score: 58.5362 bits (140), Expect = 3.223e-8 Identity = 73/364 (20.05%), Postives = 136/364 (37.36%), Query Frame = 2
Query: 1640 CEVCERVMDEVIQLAFSNHSQQVVSNFIEN--KICPRLG-FVKTECTSLIDTYLPVIFSLARSTLN-SKVICAELKLCSLISQKKI-----------NLLMKSNINDDSAK----------CKICHFVVSELLTLLTNPDIQKSIENFLENHVCPHFGAKKQECINDINQYYSLVINIITEFLKPT-LCNELRLCNSTTTSI-IMSQPLVKMSRAILSSN--------------------------CQLCEKFYFHLQKIFLKN-TPISPLTVFANVCNLEDTDETNECLKHFRNLNSIVAAFHAS-RNIGEFCKMTEMCKGSKKHHVGKKQCSWGPGFWCISRQHAAGCGPGAVKHCEMKVWN 2566
C+ C +++ +V +N S + F+++ + C RLG V C + +D Y V + L K IC C+ + + + N+L + D + C+ C FV+++ L+ N ++ + L N + +C + + +++I + P+ LC + LC + + + QP + A+L C++C+K +L+ KN T L C+ +C ++ + G C +C + K +G ++C WGP +WC + + AA C AV HC+ VWN
Sbjct: 197 CQDCMKLVSDVQTAVKTNSS--FIQGFVDHVKEDCDRLGPGVSDICKNYVDQYSEVCVQMLMHMLQQPKEICVLAGFCNEVKRVPMKTLVPATETIKNILPALEMMDPYEQNLVQAHNVILCQTCQFVMNKFSELIVNNATEELLVKGLSNACALLPDPARTKCQEVVGTFGPSLLDIFIHEVNPSSLCGVIGLCAARPELVEALEQPAPAIVSALLKEPTPPKQPAQPKQSALPAHVPPQKNGGFCEVCKKLVLYLEHNLEKNSTKEEILAALEKGCSFLPDPYQKQCDDFVAEYEPLLLEILVEVMDPGFVCSKIGVCPSAYKLLLGTEKCVWGPSYWCQNMETAARC--NAVDHCKRHVWN 556
BLAST of Proactivator polypeptide vs. Ensembl Mouse
Match: Psap (prosaposin [Source:MGI Symbol;Acc:MGI:97783]) HSP 1 Score: 57.7658 bits (138), Expect = 5.249e-8 Identity = 72/363 (19.83%), Postives = 136/363 (37.47%), Query Frame = 2
Query: 1640 CEVCERVMDEVIQLAFSNHSQQVVSNFIEN--KICPRLG-FVKTECTSLIDTYLPVIFSLARSTLNSKVICAELKLCSLISQKKI-----------NLLMKSNINDDSAK----------CKICHFVVSELLTLLTNPDIQKSIENFLENHVCPHFGAKKQECINDINQYYSLVINIITEFLKPT-LCNELRLCNSTTTSI-IMSQPLVKMSRAILSSN--------------------------CQLCEKFYFHLQKIFLKN-TPISPLTVFANVCNLEDTDETNECLKHFRNLNSIVAAFHAS-RNIGEFCKMTEMCKGSKKHHVGKKQCSWGPGFWCISRQHAAGCGPGAVKHCEMKVWN 2566
C+ C +++ +V +N S + F+++ + C RLG V C + +D Y V + + K IC C+ + + + N+L + D + C+ C FV+++ L+ N ++ + L N + +C + + +++I + P+ LC + LC + + + QP + A+L C++C+K +L+ KN T L C+ +C ++ + G C +C + K +G ++C WGP +WC + + AA C AV HC+ VWN
Sbjct: 197 CQDCMKLVSDVQTAVKTNSS--FIQGFVDHVKEDCDRLGPGVSDICKNYVDQYSEVCVQMLMH-MQPKEICVLAGFCNEVKRVPMKTLVPATETIKNILPALEMMDPYEQNLVQAHNVILCQTCQFVMNKFSELIVNNATEELLVKGLSNACALLPDPARTKCQEVVGTFGPSLLDIFIHEVNPSSLCGVIGLCAARPELVEALEQPAPAIVSALLKEPTPPKQPAQPKQSALPAHVPPQKNGGFCEVCKKLVLYLEHNLEKNSTKEEILAALEKGCSFLPDPYQKQCDDFVAEYEPLLLEILVEVMDPGFVCSKIGVCPSAYKLLLGTEKCVWGPSYWCQNMETAARC--NAVDHCKRHVWN 554
BLAST of Proactivator polypeptide vs. Ensembl Mouse
Match: Psap (prosaposin [Source:MGI Symbol;Acc:MGI:97783]) HSP 1 Score: 57.7658 bits (138), Expect = 5.320e-8 Identity = 72/363 (19.83%), Postives = 136/363 (37.47%), Query Frame = 2
Query: 1640 CEVCERVMDEVIQLAFSNHSQQVVSNFIEN--KICPRLG-FVKTECTSLIDTYLPVIFSLARSTLNSKVICAELKLCSLISQKKI-----------NLLMKSNINDDSAK----------CKICHFVVSELLTLLTNPDIQKSIENFLENHVCPHFGAKKQECINDINQYYSLVINIITEFLKPT-LCNELRLCNSTTTSI-IMSQPLVKMSRAILSSN--------------------------CQLCEKFYFHLQKIFLKN-TPISPLTVFANVCNLEDTDETNECLKHFRNLNSIVAAFHAS-RNIGEFCKMTEMCKGSKKHHVGKKQCSWGPGFWCISRQHAAGCGPGAVKHCEMKVWN 2566
C+ C +++ +V +N S + F+++ + C RLG V C + +D Y V + + K IC C+ + + + N+L + D + C+ C FV+++ L+ N ++ + L N + +C + + +++I + P+ LC + LC + + + QP + A+L C++C+K +L+ KN T L C+ +C ++ + G C +C + K +G ++C WGP +WC + + AA C AV HC+ VWN
Sbjct: 194 CQDCMKLVSDVQTAVKTNSS--FIQGFVDHVKEDCDRLGPGVSDICKNYVDQYSEVCVQMLMH-MQPKEICVLAGFCNEVKRVPMKTLVPATETIKNILPALEMMDPYEQNLVQAHNVILCQTCQFVMNKFSELIVNNATEELLVKGLSNACALLPDPARTKCQEVVGTFGPSLLDIFIHEVNPSSLCGVIGLCAARPELVEALEQPAPAIVSALLKEPTPPKQPAQPKQSALPAHVPPQKNGGFCEVCKKLVLYLEHNLEKNSTKEEILAALEKGCSFLPDPYQKQCDDFVAEYEPLLLEILVEVMDPGFVCSKIGVCPSAYKLLLGTEKCVWGPSYWCQNMETAARC--NAVDHCKRHVWN 551
BLAST of Proactivator polypeptide vs. Ensembl Mouse
Match: Psap (prosaposin [Source:MGI Symbol;Acc:MGI:97783]) HSP 1 Score: 56.9954 bits (136), Expect = 8.981e-8 Identity = 72/365 (19.73%), Postives = 136/365 (37.26%), Query Frame = 2
Query: 1640 CEVCERVMDEVIQLAFSNHSQQVVSNFIEN--KICPRLG-FVKTECTSLIDTYLPVIFSLARSTLN--SKVICAELKLCSLISQKKI-----------NLLMKSNINDDSAK----------CKICHFVVSELLTLLTNPDIQKSIENFLENHVCPHFGAKKQECINDINQYYSLVINIITEFLKPT-LCNELRLCNSTTTSI-IMSQPLVKMSRAILSSN--------------------------CQLCEKFYFHLQKIFLKN-TPISPLTVFANVCNLEDTDETNECLKHFRNLNSIVAAFHAS-RNIGEFCKMTEMCKGSKKHHVGKKQCSWGPGFWCISRQHAAGCGPGAVKHCEMKVWN 2566
C+ C +++ +V +N S + F+++ + C RLG V C + +D Y V + + K IC C+ + + + N+L + D + C+ C FV+++ L+ N ++ + L N + +C + + +++I + P+ LC + LC + + + QP + A+L C++C+K +L+ KN T L C+ +C ++ + G C +C + K +G ++C WGP +WC + + AA C AV HC+ VWN
Sbjct: 197 CQDCMKLVSDVQTAVKTNSS--FIQGFVDHVKEDCDRLGPGVSDICKNYVDQYSEVCVQMLMHMVGRQPKEICVLAGFCNEVKRVPMKTLVPATETIKNILPALEMMDPYEQNLVQAHNVILCQTCQFVMNKFSELIVNNATEELLVKGLSNACALLPDPARTKCQEVVGTFGPSLLDIFIHEVNPSSLCGVIGLCAARPELVEALEQPAPAIVSALLKEPTPPKQPAQPKQSALPAHVPPQKNGGFCEVCKKLVLYLEHNLEKNSTKEEILAALEKGCSFLPDPYQKQCDDFVAEYEPLLLEILVEVMDPGFVCSKIGVCPSAYKLLLGTEKCVWGPSYWCQNMETAARC--NAVDHCKRHVWN 557
BLAST of Proactivator polypeptide vs. UniProt/SwissProt
Match: sp|P07602|SAP_HUMAN (Prosaposin OS=Homo sapiens OX=9606 GN=PSAP PE=1 SV=2) HSP 1 Score: 89.7373 bits (221), Expect = 2.928e-17 Identity = 116/531 (21.85%), Postives = 212/531 (39.92%), Query Frame = 2
Query: 1043 CGNHHVCTSHSIKKPIASEKGVIECDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHLK--SISSQCSSLVDAYADQLITMISNHMDSKA-ICIAINACKGF-----SISQRKSA-----PNFKGIECDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIFQSEKIQCQQLVDEFFDIIL------NFVLSELD-VDKVCKVMGMCSMKKVSGIECEVCERVMDEVIQLAFSNHSQQVVSNFIENKICPRLGFVKTECTSLIDTYLPVIFSLARSTLNSKVICAELKLCSLISQKKINLLMKSNINDDSAKCKICHFVVSELLTLLTNPDIQKSIENFLENHVCPHFGAKKQECINDINQYYSLVINIITEFLKPTL-CNELRLCNSTTTSIIMSQPLVKMSRAILSSNCQLCEKFYFHLQKIFLKN-TPISPLTVFANVCNLEDTDETNECLKHFRNLNSIVAAFHASRNIGEF-CKMTEMCKGSKKHHVGKKQCSWGPGFWCISRQHAAGCGPGAVKHCEMKVWN 2566
CG C KP + CD+C+ VT A + ++ +++ K C L ++S+ C +VD+Y ++ +I M +C A+N C+ ++ +K P E A +A + Y D R P + CQ + DI FV + ++ V + C +G G+ ++C+ + + ++A Q++ + +IC +GF + T +P + + SK + L+L I KK + KS++ C++C F+V E+ L+ N +K I + + + +EC ++ Y S +++I+ E + P L C+ L LC+ T + V +++ C++C+K +L + KN T L C+ +C + ++ F C C + K +G ++C WGP +WC + + AA C AV+HC+ VWN
Sbjct: 42 CGAVKHCLQTVWNKPTVKS---LPCDICKDVVTAAGDMLKDNATEEEILVYLE-KTCDWLPKPNMSASCKEIVDSYLPVILDIIKGEMSRPGEVCSALNLCESLQKHLAELNHQKQLESNKIPELDMTEVVAPFMANIPLLLYPQDGPRSK-----------PQPKDNGDVCQDCIQMVTDIQTAVRTNSTFVQALVEHVKEECDRLG-------PGM-ADICKNYISQYSEIAI-----QMMMHMQPKEICALVGFCDEVKEMPMQTLVP-------AKVASKNVIPALELVEPI--KKHEVPAKSDVY-----CEVCEFLVKEVTKLIDNNKTEKEILDAFDKMCSKLPKSLSEECQEVVDTYGSSILSILLEEVSPELVCSMLHLCSGTRLPALT----VHVTQPKDGGFCEVCKKLVGYLDRNLEKNSTKQEILAALEKGCSFLPDPYQKQCDQFVAEYEPVLIEILVEVMDPSFVCLKIGACPSAHKPLLGTEKCIWGPSYWCQNTETAAQC--NAVEHCKRHVWN 524 HSP 2 Score: 55.0694 bits (131), Expect = 2.326e-6 Identity = 86/438 (19.63%), Postives = 162/438 (36.99%), Query Frame = 2
Query: 581 CTKCKQVFQFLESMFSKVSPDEFKFLIESLICNQLP-SEVQSTCKLLLDGYVTQLYNLIKSLLD-GDRICPLIHLCSSENTQITLGSWQCQTCLDVIVVLRKDLAGVSAP--------------------------------EIVSLIKSNV-----------------CSKLNFFEKGICNSYVSNLVPTFVNELHSTLTPQQICGNHHVCTS--------------------------HSIKKPIASEKGVIECDLCEIGVTGAKSFISKPENMNKVKSFIKS--KICSHL-KSISSQCSSLVDAYADQLITMISNHMDSKAICIAINACKGF---SISQRKSAPNFKGIECDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIFQSEKIQCQQLVDEFFDIILNFVLSELDVDKVCKVMGMC--SMKKVSGIE 1639
C CK V M + +E + C+ LP + ++CK ++D Y+ + ++IK + +C ++LC E+ Q L Q L+ + D+ V AP ++V+ I++ V C +L IC +Y+S + ++ + P++IC C IKK K + C++CE V I +N K + + K+CS L KS+S +C +VD Y +++++ + + +C ++ C G +++ + P G C+ C V + + + K I L+ + + QC Q V E+ +++ ++ +D VC +G C + K + G E
Sbjct: 63 CDICKDVVTAAGDMLKDNATEEEILVYLEKTCDWLPKPNMSASCKEIVDSYLPVILDIIKGEMSRPGEVCSALNLC--ESLQKHLAELNHQKQLESNKIPELDMTEVVAPFMANIPLLLYPQDGPRSKPQPKDNGDVCQDCIQMVTDIQTAVRTNSTFVQALVEHVKEECDRLGPGMADICKNYISQYSEIAI-QMMMHMQPKEICALVGFCDEVKEMPMQTLVPAKVASKNVIPALELVEPIKKHEVPAKSDVYCEVCEFLVKEVTKLI---DNNKTEKEILDAFDKMCSKLPKSLSEECQEVVDTYGSSILSILLEEVSPELVCSMLHLCSGTRLPALTVHVTQPKDGGF-CEVCKKLVGYLDRNLEKNSTKQEILAALEKGCSFLPDPYQKQCDQFVAEYEPVLIEILVEVMDPSFVCLKIGACPSAHKPLLGTE 493
BLAST of Proactivator polypeptide vs. UniProt/SwissProt
Match: sp|P26779|SAP_BOVIN (Prosaposin OS=Bos taurus OX=9913 GN=PSAP PE=1 SV=3) HSP 1 Score: 83.9593 bits (206), Expect = 2.497e-15 Identity = 106/511 (20.74%), Postives = 191/511 (37.38%), Query Frame = 2
Query: 1292 NHMDSKAICIAINACKGFSISQRKSAPNFKGIECDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIFQSEKIQ--CQQLVDEFFDIILNFVLSELD-VDKVCKVMGMCSM---------------------------------------------------KKVSGIECEVCERVMDEVIQLAFSNHSQQVVSNFIENKI------CPRLGFVKTE-CTSLIDTYLPVIFSLARSTLNSKVICAELKLCSLISQKKINLLMKSN---------------INDDSAK------CKICHFVVSELLTLLTNPDIQKSIENFLENHVCPHFGAK-KQECINDINQYYSLVINIITEFLKPTL-CNELRLCNSTTTSIIMSQPLVKMSRAILSSNCQLCEKFYFHLQKIFLKN-TPISPLTVFANVCNLEDTDETNECLKHFRNLNSIVAAFHASRNIGEF-CKMTEMCKGSKKHHVGKKQCSWGPGFWCISRQHAAGCGPGAVKHCEMKVWN 2566
++ + A C A+ C + S P K + CD C +T + D A + I +L+ R C + C+++VD + +IL+ + ++ +VC + +C KK +G C+ C +++ +V + +N S F+E + C RLG ++ C + I+ Y V + + K IC C + + + L+ + I D A C++C FVV E+ L+ N ++ I + L+ VC ++C ++ Y S +++I+ + P L C+ L LC+S + V++ C++C+K +L + KN T L C+ +C + ++ F C C + K +G ++C WGP +WC + + AA C AV+HC VWN
Sbjct: 34 QNVKTAADCGAVQHC----LQTVWSKPTVKSLPCDICKDVITAAGNLLKDNATEQEILMYLE-RTCDWLPKPNMSASCKEIVDSYLPVILDMIKGQMSHPGEVCSALNLCESLQKHLAELNHQKQLESNQIPELDMAEVVAPFMANIPFLLYPQDGSHSKPQPKKANGNVCQDCIQLVTDVQEALRTN------STFVEALVDHAKEECDRLGPGMSDMCKNYINQYSEVAIQMVMH-MQPKEICVLAGFCDEVKEMPMKTLVPAEVVSENVIPALGLVEPIKKDPAPAKADIYCEVCEFVVKEVAKLIDNNRTEEEILHALDK-VCSKLPTSLAEQCQEVVDTYGSSILSILLDEASPELVCSMLHLCSSRG----LPAATVRVMPRKDGGFCEVCKKLVGYLDRNLEKNSTKEQILAALEKGCSFLPDQYRKQCDQFVTEYEPVLIEILVEVMDPSFVCLKIGACPAAHKPLLGAEKCVWGPSYWCQNMESAALC--NAVEHCRRHVWN 525 HSP 2 Score: 60.077 bits (144), Expect = 7.603e-8 Identity = 111/544 (20.40%), Postives = 195/544 (35.85%), Query Frame = 2
Query: 86 YILFKMRFLIFGFLISVCYAFDFDCLKGPTYWCATYQNAEKCDRITECFMGQGDQKNKVDISHHKNGDYCDACMKFVQSVEAHIPKISEQEFVNLAGNLICNNLKGKSQLDCKKEVNFFAPLIYRELSMEATPEKICGLVQVCKNTNAMSFSKIFNSIKFQNDDVCTKCKQVFQFLESMFSKVSPDEFKFLIESLICNQLPSEVQSTCKLLLDGYVTQLYNLIKSLL---DGDRICPLIHLCSSENTQITLGSWQCQTCL----DVIVVLRKDLAGVSAPEIVSLIKSNVCSKLNFFEKGICNSYVSN------------------LVPTFVNELHS----TLTPQQICGNHHVCT---SHSIKKPIASEKGVIECDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHLK-SISSQCSSLVDAYADQLITMISNHMDSKAICIAINAC--KGFSISQRKSAPNFKGIECDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIFQSEKIQCQQLVDEFFDIILNFVLSELDVDKVCKVMGMC 1612
Y F + L+ G L S +C +G WC + A C + C + + CD C ++ + AGNL+ +N ++E+ L+Y E + + P+ + CK I + IK Q C ES+ ++ + +ES NQ+P +L + V I LL DG P N + CQ C+ DV LR + V A +V K C +L +C +Y++ ++ F +E+ TL P ++ + + IKK A K I C++CE V I ++ + K+CS L S++ QC +VD Y +++++ + + +C ++ C +G + + P G C+ C V + + + K I L+ + + QC Q V E+ +++ ++ +D VC +G C
Sbjct: 2 YSFFVLASLLGGALASPVLGLR-ECTRGSAVWCQNVKTAADCGAVQHCLQTVWSKPTVKSLP-------CDIC----------------KDVITAAGNLLKDNAT-------EQEI-----LMYLERTCDWLPKP--NMSASCKEIVDSYLPVILDMIKGQMSHPGEVCS-ALNLCESLQKHLAELNHQKQLES---NQIP-------ELDMAEVVAPFMANIPFLLYPQDGSHSKPQP---KKANGNV------CQDCIQLVTDVQEALRTNSTFVEA--LVDHAKEE-CDRLGPGMSDMCKNYINQYSEVAIQMVMHMQPKEICVLAGFCDEVKEMPMKTLVPAEVVSENVIPALGLVEPIKKDPAPAKADIYCEVCEFVVKEVAKLIDNNRTEEEILHAL-DKVCSKLPTSLAEQCQEVVDTYGSSILSILLDEASPELVCSMLHLCSSRGLPAATVRVMPRKDGGFCEVCKKLVGYLDRNLEKNSTKEQILAALEKGCSFLPDQYRKQCDQFVTEYEPVLIEILVEVMDPSFVCLKIGAC 483
BLAST of Proactivator polypeptide vs. UniProt/SwissProt
Match: sp|O13035|SAP_CHICK (Prosaposin OS=Gallus gallus OX=9031 GN=PSAP PE=1 SV=1) HSP 1 Score: 74.3294 bits (181), Expect = 2.608e-12 Identity = 113/518 (21.81%), Postives = 197/518 (38.03%), Query Frame = 2
Query: 155 DCLKGPTYWCATYQNAEKCDRITECFMGQGDQKNKVDISHHKNGDYCDACMKFVQSVEAHIPKISEQEFVNLAGNLICNNLKGKS-QLDCKKEVNFFAPLIYRELSME-ATPEKICGLVQVC----KNTNAMSFSKIFNSIKFQNDDVCTKCKQVFQFLES---------MFSKVSPDEFKFLIESLI--CNQLPSEVQSTCKLLLDGYVTQLYNLIKSLL-----DGDRICP-LIHLCSSENTQITLGSWQCQTCLDVIVVLRKDLAGVSAPEIVSLIKSNVCSKLNFFEKGICNSYVSNLVPTFVNELHSTLTPQQICGNHHVCTSHSIKKPIASEKGVIECDLCEIGVTGAKSFISKPENMNKVKSFIKSK---ICSHLK-SISSQCSSLVDAYADQLITMISNHMDSKAICIAINACKGFSISQRKSAPNFKGIECDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIF-QSEKIQCQQLVDEFFDIILNFVLSELDVDKVCKVMGMCSMKK 1624
DC KGP WC + + A +C + C Q+N V N CD C + V V + ++ + C L + +CK+ V+ + P+I + E PE +C + +C K+ AM K S K D F L S ++ + P + E + C +L ++VQ V +KSL+ + DR+ P + +C S ++ + D+ + + + +I +++ G C S S VP TL P Q+ H +++K EK C++CE V + NK + I + +C L S+ QC ++ Y LI M+ + +A+C+ + C Q+ G CD C + V + + A T I+ L+ ++C +S QC Q V+++ +++ + +D VC +G+C K
Sbjct: 25 DCAKGPEVWCQSLRTASQCGAVKHC------QQN-VWSKPAVNSIPCDLCKELVTVVGKVLKDNGTEDEIRSYLEKRCEFLPDQGLASECKEIVDSYLPVIMDMIKEEFDKPEVVCSALSLCQSLQKHLAAMKLQKQLQSNKIPELD--------FSELTSPFMANVPLLLYPQDKPKQKSKATEDVCQDCIRLVTDVQEA--------VRTNATFVKSLVAHAKEECDRLGPGMSDMCKSYISEYS----------DLAIQMMMHMKDQQPKDICAMV-------------GFCPSVKS--VPL------QTLVPAQVV---HEVKMETVEKATVQEKTFSVCEICETMVKEVTGLLES----NKTEEEIVHEMEVVCYLLPASVKDQCKDFIEVYGQALIDMLLEATNPEAVCVMLKCCAANKPPQQPVVVKPAGGFCDICKMIVAYADKELEKNATTTEIEALLE-KVCHFLPESVSDQCVQFVEQYEPVVVQLLAEMMDPTFVCTKLGVCGAAK 480 HSP 2 Score: 71.633 bits (174), Expect = 1.564e-11 Identity = 97/458 (21.18%), Postives = 180/458 (39.30%), Query Frame = 2
Query: 1043 CGNHHVCTSHSIKKPIASEKGVIECDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHL--KSISSQCSSLVDAYADQLITMISNHMD-SKAICIAINACKGFS-------------------------------------ISQRKSAPNFKGIE--CDACHLAVTKIKEYISDPARKTSIKNFLKM------RICPIFQSEKIQCQQLVDEFFDIILNFVLSELDVDK--VCKVMGMC-SMK----------------KVSGIE-----------CEVCERVMDEVIQLAFSNHSQQVVSNFIENKICPRL-GFVKTECTSLIDTYLPVIFSLARSTLNSKVICAELKLCSLISQKKINLLMKSNINDDSAKCKICHFVVSELLTLLTNPDIQKSIENFLENHVCPHFGAK--KQECINDINQYYSLVINIITEFLKPT-LCNELRLCNSTTTSII 2170
CG C + KP + I CDLC+ VT + ++++S+++ + C L + ++S+C +VD+Y ++ MI D + +C A++ C+ Q K K E C C VT ++E + A T +K+ + R+ P C+ + E+ D+ + ++ D +C ++G C S+K K+ +E CE+CE ++ EV L SN +++ + + +E +C L VK +C I+ Y + + N + +C LK C+ + +++K C IC +V+ L IE LE VC HF + +C+ + QY +V+ ++ E + PT +C +L +C + ++
Sbjct: 43 CGAVKHCQQNVWSKPAVNS---IPCDLCKELVTVVGKVLKDNGTEDEIRSYLEKR-CEFLPDQGLASECKEIVDSYLPVIMDMIKEEFDKPEVVCSALSLCQSLQKHLAAMKLQKQLQSNKIPELDFSELTSPFMANVPLLLYPQDKPKQKSKATEDVCQDCIRLVTDVQEAVRTNA--TFVKSLVAHAKEECDRLGPGMSD---MCKSYISEYSDLAIQMMMHMKDQQPKDICAMVGFCPSVKSVPLQTLVPAQVVHEVKMETVEKATVQEKTFSVCEICETMVKEVTGLLESNKTEEEIVHEMEV-VCYLLPASVKDQCKDFIEVYGQALIDMLLEATNPEAVCVMLKCCAANKPPQQPVVVKPA----GGFCDICKMIVAYADKELEKNATTTEIEALLEK-VC-HFLPESVSDQCVQFVEQYEPVVVQLLAEMMDPTFVCTKLGVCGAAKKPLL 484
BLAST of Proactivator polypeptide vs. UniProt/SwissProt
Match: sp|Q6NUJ1|SAPL1_HUMAN (Proactivator polypeptide-like 1 OS=Homo sapiens OX=9606 GN=PSAPL1 PE=2 SV=2) HSP 1 Score: 73.559 bits (179), Expect = 4.710e-12 Identity = 113/530 (21.32%), Postives = 206/530 (38.87%), Query Frame = 2
Query: 1043 CGNHHVCTSHSIKKPIASEKGVIECDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHLKS--ISSQCSSLVDAYADQLITMISNHMDSKA--ICIAINACKGFSISQRKSAPNFKGIECDACHLAVTKIKEYISDPARKTSIKNFL---KMRICPIFQSEKIQCQQLVDEFFD----IILNFVLSELDVDKVCKVMGMCSMKKVSGIECEVCERVMDEVIQLAFSNHSQQVVSNFIENKICPRLGFVKTECTSLIDTYLPVIFSLARSTLNSKVICAELKLCSLISQKKINLLMKSNINDDSAKCKICHFVVSELLTLLTNPDIQKSIENFLENHVCPHFGAK-KQECINDINQYYSLVINIITEFLKPTLCNELRLCNSTTTSIIMSQ-----PLVKMSRAILSSNCQLCEKFY-FHLQKIFLKNTPISPLTVFANVCNLEDTDETNECLKHF--RNLNSIVAAFHASRNIGEFCKMTEMCKGSKKHHVGKKQCSWGPGFWCISRQHAAGCGPGAVKHCEMKVWNQM 2572
CG C KP A + CD+C+ A + ++ + + + + K C L S S+ C +VDA++ +++M+ DS +C A++ C+ QR HLA + +S ++ F+ + P E CQ V + + N L++L++ + C+ +G G+ +C+ + F + Q + ++C + GF + L L + V EL L +K+ + MK+ + C++C VV +L L + + I + LE VC A +ECI ++ Y ++ ++ + +C +RLC + + + P + S C C++ + K+T L F C++ +C KHF + ++ + + CK C G + +G QC+ GP FWC S++ A C AV+HC+ VW +M
Sbjct: 43 CGAVGYCQGAVWNKPTAKS---LPCDVCQDIAAAAGNGLNPDATESDILALVM-KTCEWLPSQESSAGCKWMVDAHSSAILSMLRGAPDSAPAQVCTALSLCEPL---QR--------------HLATLR---PLSKEDTFEAVAPFMANGPLTFHPRQAPEGALCQDCVRQVSRLQEAVRSNLTLADLNIQEQCESLG-------PGLAV-LCKNYL-----FQFFVPADQALRLLPPQELCRKGGFCEE---------LGAPARLTQVVAMDGVPSLELGL----PRKQSEMQMKAGVT-----CEVCMNVVQKLDHWLMSNSSELMITHALER-VCSVMPASITKECIILVDTYSPSLVQLVAKITPEKVCKFIRLCGNRRRARAVHDAYAIVPSPEWDAENQGSFCNGCKRLLTVSSHNLESKSTKRDILVAFKGGCSILPLPYMIQC-KHFVTQYEPVLIESLKDMMDPVAVCKKVGACHGPRTPLLGTDQCALGPSFWCRSQEAAKLC--NAVQHCQKHVWKEM 513
BLAST of Proactivator polypeptide vs. UniProt/SwissProt
Match: sp|Q8C1C1|SAPL1_MOUSE (Proactivator polypeptide-like 1 OS=Mus musculus OX=10090 GN=Psapl1 PE=1 SV=2) HSP 1 Score: 73.1738 bits (178), Expect = 6.291e-12 Identity = 108/523 (20.65%), Postives = 197/523 (37.67%), Query Frame = 2
Query: 1061 CTSHSIKKPIASEKGVIECDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHLKS--ISSQCSSLVDAYADQLITMISN--HMDSKAICIAINACKGFSISQRKSAPNFKGIECDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIFQSEKIQ------CQQLVDEFFDII-LNFVLSELDVDKVCKVMGMCSMKKVSGIECEVCERVMDEVIQLAFSNHSQQVVSNFIENKICPRLGFVKTECTSLIDTYLPVIFSLARSTLNSKVICAELKLCSLISQKKINLLMKSNINDDSAKCKICHFVVSELLTLLTNPDIQKSIENFLEN--HVCPHFGAKKQECINDINQYYSLVINIITEFLKPTLCNELRLCNS-----TTTSIIMSQPLVKMSRAILSSNCQLCEKFY-FHLQKIFLKNTPISPLTVFANVCNLEDTDETNECLKHFRNLNSI-VAAFHASRNIGEFCKMTEMCKGSKKHHVGKKQCSWGPGFWCISRQHAAGCGPGAVKHCEMKVWNQ 2569
C S KP + C +C+ A + ++ + + + + K C L S S++C +V+ ++ +++M+S D ++C A+ C+ QR HLA T + ++ + FL S+ + C QL+ D + N L+E+ V C+ MG G+ +CE + A +Q + ++C + GF + E L R V E+++ + L M+ + C +C +V EL L + I + LE V P Q+CI ++ Y ++ ++++ +C ++LC S + + + + P + + S CQ C++ Q + K+T L F C + +C + + + + N + CK C G K +G QC GP FWC S + A C A++HC+ VW +
Sbjct: 49 CQSAVWNKPTVKS---LPCSVCQDVAAAAGNGVNPGATESDILTSVM-KTCEWLPSQESSAKCKWMVNNHSAAVLSMLSGAQETDLASVCTALTLCEPL---QR--------------HLAETTSERPLTQEDANEVMAPFLSNGALSFHPSQMPEGAVCHDCVQLISLLQDALESNLTLAEVTVQNQCQSMG-------PGLA-ALCENYIHRQFVPA-----KQTLQGLPPQEVCRKGGFCERESAHW----------LTRVAAVDGVPSLEMEM-----PRTNELQMQLGLT-----CDVCLNLVQELDKWLVTNSTEALISHTLERVCTVVPE--PLVQQCITLVDTYSPELVQLMSKVTPEKVCETIKLCGSKRRARSISRAVATTPSLPVDEENQGSFCQGCKRLLGMSSQNLDHKSTKRDILNAFKGGCRILPLPYVMQCNRFVAEYEPVLIESLKFMMNPTDLCKKMGACHGPKTPLLGTDQCVMGPSFWCKSPEAAEMC--NALEHCQRLVWKK 513
BLAST of Proactivator polypeptide vs. TrEMBL
Match: A0A2R5L700 (Putative prosaposin OS=Ornithodoros turicata OX=34597 PE=4 SV=1) HSP 1 Score: 186.422 bits (472), Expect = 1.574e-44 Identity = 189/814 (23.22%), Postives = 348/814 (42.75%), Query Frame = 2
Query: 293 CDACMKFVQSVEAHIPKISEQEFVNLAGNLICNNLKGKSQLDCKKEVNFFAPLIYRELSMEATPEKICGLVQVCKNTNAMSFSKIFNSIKF----QNDDVCTKCKQVFQFLESMFSK-VSPDEFKFLIESLICNQLPSEVQSTCKLLLDGYVTQLYNLIKSLLDGDRICPLIHLCSSENTQITLGSWQCQTCLDVIVVLRKDLAGVSAPEIVSLIKSNVCSKLNFFEKGICNSYVSNLVPTFVNELHSTLTPQQICGNHHVCTS--HSIKKPIASEKGV----IECDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHL-KSISSQCSSLVDAYADQLITMISNHMDSKAICIAINAC--KGFSISQRKS--APNFKGIECDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIFQSEKIQCQQLVDEFFDIILNFVLSELDVDKVCKVMGMCS----------MKKVS---GIECEVCERVMDEVIQLAFSNHSQQVVSNFIENKIC---PRLGFVKTECTSLIDTYLPVIFSLARSTLNSKVICAELKLCSLISQKKINLLMKSNINDDSAKCKIC----HFVVSELLTLLTNPDIQKSIENFLENHVCPHFGAK-KQECINDINQYYSLVINIITEFLKPTL-CNELRLCNSTTTSIIMSQPLVKMSRAILS---SNCQLCEKFYFHLQKIFLKNTPISP--LTVFANVCNLEDTDETNECLKHFRNLNSIVAAFHA--SRNIGEF------CKMTEMCKGSKK----HHVGKKQCSWGPGFWCISRQHAAGCGPGAVKHCEMKVWNQ 2569
C+ C+ V+ V+A + ++ V A C + C + V+ + + L E P +CGL+ +C + S + +I Q+ C CK V +F E+ + V+ D +++++ L CNQ + CK L+D + + + + IC L +C + S C C +++ V+ L V E ++ VC+ + + C V+ V+ + LTP Q+C C + +I KP ASEK EC LC+ + + + ++K ++ ++C + KS +C +++ Y D L+++I +C A+ C G + + S +P +G EC C V + + D + +K L+ + QS + +C +V+ +FD++++ +L + D+VC+ +G+C ++K+S EC +C+ V+ + N ++Q + + +++C P+ K EC +++ TY ++ SL IC L LC S + C C HF+ +EL+ T I++++ + +C + +EC + +Y + VI ++ + L P++ C +R C LVK S A+ + C C F ++T +VF VC EC S+V A+ RNIG+ C+ +MC +++ H +G K+C++GP +WC S HA+ C A ++C+ KVW Q
Sbjct: 414 CELCIYLVKYVDAVLENKRDEAAVTHALEEACKLVPPHMHDRCVQLVDKYVDELIHILVEEDDPNLVCGLLGLCTGQTSTSSVQSHTAITAEKNPQDSIECEVCKAVIEFFENNLPEVVTKDVVEYVLDEL-CNQFSGDKAQECKNLVDKNIELILKYLTGEVAATAICRLAQMCEAPQVATKGVSPDCVVCKELVQVVYDQLKDVRTEEAIAKELERVCNLVPSSSRRTCVDIVNTYFEALVSLILQELTPDQVCSALKFCGAPGKAILKP-ASEKSPKHEDPECTLCKQVFQLVYNELKDNKTQEEIKQVLE-EVCDLVPKSSRQECVNMISNYFDLLVSLILQDFTPDEVCKALGICPPSGKVVPKPASTKSPKVEGSECTLCMDVVQFVYNELKDTKTEEEVKRVLEEVCTLVPQSSRQECVNMVNTYFDLLVSLILQDFTPDEVCQTLGLCPSSVKVVTKPKIQKISKDDNPECTLCKDVVQFIYAELKDNKTEQAIKEVL-DEVCNWVPQSS--KQECLNMVSTYFDLLVSLILQDFTPDQICQTLTLCPSSSHGDV-------------VCTFCQYALHFIQTELMDNKTEEKIKEAL-----DQLCARLPKEISEECQAFVEEYGAAVIVLLAQELDPSIVCVAVRACPKGG--------LVKQSMALHNLYFDKCDTC-SLVIGWADRFTQDTKTDEELASVFERVCKAVPAGVKQEC-------QSLVKAYGPFILRNIGQLGNSRQVCQAIDMCPKAQRAKSAHLLGSKKCTFGPAYWCHSEAHASAC--NAHEYCKKKVWKQ 1185 HSP 2 Score: 85.8853 bits (211), Expect = 3.917e-13 Identity = 78/371 (21.02%), Postives = 149/371 (40.16%), Query Frame = 2
Query: 281 NGDYCDACMKFVQSVEAHIPKISEQEFVNLAGNLICNNLKGKSQLDCKKEVNFFAPLIYRELSMEATPEKICGLVQVCKNTNAMSFSKIFNSIKFQNDDVCTKCKQVFQFLESMFSKVSPDEFKFLIESLICNQLPSEVQSTCKLLLDGYVTQLYNLIKSLLDGDRICPLIHLCSSENTQITLGSWQCQTCLDVIVVLRKDLAGVSAPEIVSLIKSNVCSKLNFFEKGICNSYVSNLVPTFVNELHSTLTPQQICGNHHVCTSHSIKKPIASEKGVI--ECDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHLKS-ISSQCSSLVDAYADQLITMISNHMDSKAICIAINACKGFSISQRKSAPNFKG 1384
G C CM VQ V + +E V +C + S+ +C VN + L+ + + TP+++C + +C ++ + I ++ CT CK V QF+ + ++ + +CN +P + C ++ Y L +LI D+IC + LC S + G C C + ++ +L E + +C++L C ++V + L L P +C C + K + + +CD C + + A F + ++ S + ++C + + + +C SLV AY ++ I +S+ +C AI+ C +QR + + G
Sbjct: 789 EGSECTLCMDVVQFVYNELKDTKTEEEVKRVLEEVCTLVPQSSRQECVNMVNTYFDLLVSLILQDFTPDEVCQTLGLCPSSVKVVTKPKIQKISKDDNPECTLCKDVVQFIYAELKDNKTEQAIKEVLDEVCNWVPQSSKQECLNMVSTYFDLLVSLILQDFTPDQICQTLTLCPSSSH----GDVVCTFCQYALHFIQTELMDNKTEEKIKEALDQLCARLPKEISEECQAFVEEYGAAVIVLLAQELDPSIVCVAVRACPKGGLVKQSMALHNLYFDKCDTCSLVIGWADRFTQDTKTDEELASVFE-RVCKAVPAGVKQECQSLVKAYGPFILRNIGQLGNSRQVCQAIDMCPK---AQRAKSAHLLG 1151 HSP 3 Score: 70.4774 bits (171), Expect = 1.775e-8 Identity = 110/538 (20.45%), Postives = 208/538 (38.66%), Query Frame = 2
Query: 965 ICNSYVSNLVPTFVNELHSTLTPQQICGNHHVCTSHSIKK-------------------PIAS--EKGVIECDLCEIGVTGAKSFISK-PENMNKVKSFIKSKIC-SHLKSISSQCSSLVDAYADQLITMISNHMDSKAICIAINACKGFSISQRKSAPNFKG---IECDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIFQSEKIQCQQLVDEFFDIILNFVLSELDVDKVCKVMGMC--SMKKVSGIECEVCERVMDEVIQLAFSNHSQQVVSNFIENKICPRLGFVKTECTSLIDTYLPVIFSLARSTLNSKVICAELKLCSLISQKKINLLMKSNINDDSAKCKICHFVVSELLTLLTNPDIQKSIENFLENH---VCPHFGAKKQECINDINQYYSLVINIITEFLKPTL-CNELRLCNS-TTTSIIMSQPLVKMSRAILSS-NCQLC----EKFYFHLQKIFLKNTPISPLTVFANVCNLEDTDETNECLKHF-RNLNSIVAAFHASRNIGEFCKMTEMCKGSKKHHVG 2461
+C V +P ++ L S + P Q+C +C + ++ P+ S + EC C + AK+ I PE ++ + K+C H K ++C +V Y + ++ C+ I C S + + + P KG + CD C V ++ ++ + +K + + C S +CQ L+DE+FD+ ++L L+ ++ C +G+C S+ +V + K+ P + K L + S+ +S I +KL I K +K + +C++C ++V + +L N + ++ + LE V PH + C+ +++Y +I+I+ E P L C L LC T+TS + S + + S C++C E F +L ++ K+ V +CN D+ EC +N+ I+ C++ +MC+ + G
Sbjct: 113 MCIRMVDEFIPDLIDMLVSRMDPDQVCTIAGLCNPNFFRQLRMQQNLIFSAFQSRIFLPPLTSTASRRTGECSRCLNTLQPAKAIIRLLPEAQVELLAL---KMCQDHHKDNLTECPLIVKKYFSPVYDYFYETAANQT-CVDIRRCPP-SETLKPNVPLVKGDDDLTCDFCKQLVGSLRNFLEANTTQDELKQAI-LAFCEDLGSAAEECQNLLDEYFDMFYTYLLEALEPERFCSTIGLCQTSLLRVPLV-------------------------------KLFPAVPATKVHLQKL-------QYPKKASSAHSPDITPLVKLMPGIPVK----YLKKDEPKKQVECELCIYLVKYVDAVLENKRDEAAVTHALEEACKLVPPHMHDR---CVQLVDKYVDELIHILVEEDDPNLVCGLLGLCTGQTSTSSVQSHTAITAEKNPQDSIECEVCKAVIEFFENNLPEVVTKDV---VEYVLDELCNQFSGDKAQECKNLVDKNIELILKYLTGEVAATAICRLAQMCEAPQVATKG 596
BLAST of Proactivator polypeptide vs. TrEMBL
Match: K1QNB6 (Proactivator polypeptide OS=Crassostrea gigas OX=29159 GN=CGI_10021835 PE=4 SV=1) HSP 1 Score: 184.882 bits (468), Expect = 4.958e-44 Identity = 186/850 (21.88%), Postives = 362/850 (42.59%), Query Frame = 2
Query: 254 NKVDISHHKNGDYCDACMKFVQSVEAHIPKISEQEFVNLAGNLICNNLKGKSQLDCKKEVNFFAPLIYRELSMEATPEKICGLVQVCKNTNAMSFSKIFNSIKF----------QNDDVCTKCKQVFQFLESMFSKVSPDEFKFLIESLICNQLPSEVQSTCKLLLDGYVTQLYNLIKSLLDGDRICPLIHLCSSENTQITL-----GSWQCQTCLDVIVVLRKDLAGVSAPEIVSLIKSNVCSKLNFFEKGI-CNSYVSNLVPTFVNELHSTLTPQQICGNHHVCTS---------HSIK---------KPIASEKGVIECDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHLKS-ISSQCSSLVDAYADQLITMISNHMDSKAICIAINACKGFSISQRKSAPNFKGIECDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIFQSEKIQCQQLVDEFFDIILNFVLSELDVDKVCKVMGMC--SMKKVS---GIECEVCERVMDEVIQLAFSNHSQQVVSNFIENKICPRL-GFVKTECTSLIDTYLPVIFSLARSTLNSKVICAELKLCSLISQKKINL-----------LMKSNINDDSAKCKICHFVVSELLTLLTNPDIQKSIENFLENHVCPHFGAKKQECINDINQYYSLVINIITEFLKPTLCNELRLCNSTTTSIIMSQPLVKMSRAILSSNCQLCEKFYFHLQKIFLKNTPISPL-TVFANVCNLEDTDETNECLKHFRNLNSIVAA-FHASRNIGEFCKMTEMCKG----------------------------SKKHHVGKKQCSWGPGFWCISRQHAAGCGPGAVKHCEMK 2557
NK I + D C M V+ ++ P E+ + +L C L G CK V+ +A +++ ++ E P +C ++ CK T ++ I I+ + ++C C+ V L+SM K S + + +C +LP++ C + Y + +LI LD +C ++ LC+SE+ ++ + + +C C ++ + L G E + +VC +L+ + C+++V ++ L S + P+++C +CT+ H +K + S +EC +CE ++ + + + + +++ +K +CS L S IS +C+ V+ YAD +I ++ D K +C A+N C+ + ++ A +EC C ++++ + + + +I+N LKM + + +C V+++ D+++N ++ E D VCK + +C S K S +EC +CE +M EV + N S++ + N ++ +C +L + ECT ++ Y ++ +L + K +C L LCS QKK N+ + + D+ KC++C +++ L L +++E L + VC FL PT E R+ ++ S+ + C++CE +L + +N + + + VCN+ T EC ++A F + + C+ ++C S++ +GKK+C++GP +WC S ++A C A +HC K
Sbjct: 318 NKAKIGGVEECDLCKEVMSKVRDMDRD-PTNQEKIKAYIKTDL-CEKL-GSIADQCKTAVDEYADILFELIANELDPTTVCTMLGFCKTTYYVAAKPILPGIQLKPKVNVQEKPKAGELCDLCQMVINELDSMILKNSTTQEVEKALNSVCKRLPAKYSDDCSDFVKQYAPAILDLIGQELDPLTVCTVLKLCTSESKKLVIQKPVESTTECVLCEMIMKEVDTILQGSKVKEEIESALKSVCDRLSTGDLAKECSNFVMQYTEILIDLLKS-MPPKEVCSKLGLCTADKAPAKQQLHHVKLVSDKHNSVSHLHSVDASVECVVCEYIMSEVDNILKENRSREAIENALKM-VCSKLPSTISQECTDFVNQYADLVINLLIQEGDPKTVCKALNLCQASAKAKSVDA----SVECVLCEYIMSEVDNILKENRSREAIENALKMVCSKLPATISQECTDFVNQYADLVINLLIQEGDPKTVCKALNLCQASAKAKSVDASVECVLCEYIMSEVDNILKENRSREAIENALK-MVCSKLPATISQECTDFVNQYADLVINLLIQEGDPKTVCKALNLCS-SKQKKTNINSIRFLPIPGQVASLRLGDE--KCEVCEVILNYLDEALKENKTVQAVEALL-DQVC--------------------------NFL-PTTFKEERIFLTSVASLRLG-----------DEKCEVCEVILNYLDEALKENKTVQAVEALLDQVCNILPTTFKEECDTIVAQYGPVIAQLFSQLLDPKKICQEIKLCPSKYGVKQSLINHLMKKTKPAPKDTRLFFKSRQQKLGKKECTYGPSYWCASLKNAQKC--QAEEHCRTK 1113 HSP 2 Score: 161.77 bits (408), Expect = 1.155e-36 Identity = 134/513 (26.12%), Postives = 237/513 (46.20%), Query Frame = 2
Query: 905 EIVSLIKSNVCSKLNFFEKGICNSYVSNLVPTFVNELHSTLTPQQICGNHHVCTSHSIKKPIASE---------------KGVIECDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHLKSISSQCSSLVDAYADQLITMISNHMDSKAICIAINACK-GFSISQRKSAPNFK-------------GIECDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIFQSE-KIQCQQLVDEFFDIILNFVLSELDVDKVCKVMGMCS--MKKV-------SGIECEVCERVMDEVIQLAFSNHSQQVVSNFIENKICPRL--GFVKTECTSLIDTYLPVIFSLARSTLNSKVICAELKLCS--------------LISQKKINLLMKSNINDDSAKCKICHFVVSELLTLLTNPDIQKSIENFLENHVCPHFGAK-KQECINDINQYYSLVINIITEFLKP-TLCNELRLCNSTTTSIIMSQPLVKMSRAILSSNCQLCEKFYFHLQKIFLKN 2272
+I ++K+ +C++L E+G+C+ V LVP + L S + PQ C C +I K + + GV ECDLC+ ++ + P N K+K++IK+ +C L SI+ QC + VD YAD L +I+N +D +C + CK + ++ + P + G CD C + + ++ I + ++ L +C ++ C V ++ IL+ + ELD VC V+ +C+ KK+ S EC +CE +M EV + + ++ + + +++ +C RL G + EC++ + Y ++ L +S + K +C++L LC+ L+S K N + + D S +C +C +++SE+ +L +++IEN L+ VC + QEC + +NQY LVIN++ + P T+C L LC ++ K S C LCE + I +N
Sbjct: 244 QIKDMLKNVLCTQLPS-EEGLCDFIVDELVPQLLQTLGSEVDPQVFCNMMGFCNGTNINKALVTRIQYRTELKKLNKAKIGGVEECDLCKEVMSKVRDMDRDPTNQEKIKAYIKTDLCEKLGSIADQCKTAVDEYADILFELIANELDPTTVCTMLGFCKTTYYVAAKPILPGIQLKPKVNVQEKPKAGELCDLCQMVINELDSMILKNSTTQEVEKALN-SVCKRLPAKYSDDCSDFVKQYAPAILDLIGQELDPLTVCTVLKLCTSESKKLVIQKPVESTTECVLCEMIMKEVDTILQGSKVKEEIESALKS-VCDRLSTGDLAKECSNFVMQYTEILIDLLKS-MPPKEVCSKLGLCTADKAPAKQQLHHVKLVSDKH-NSVSHLHSVDASVECVVCEYIMSEVDNILKENRSREAIENALK-MVCSKLPSTISQECTDFVNQYADLVINLLIQEGDPKTVCKALNLCQAS----------AKAKSVDASVECVLCEYIMSEVDNILKEN 740 HSP 3 Score: 160.999 bits (406), Expect = 1.847e-36 Identity = 182/828 (21.98%), Postives = 318/828 (38.41%), Query Frame = 2
Query: 155 DCLKGPTYWCATYQNAEKCDRITECFMGQGDQKNKVDISHHKNGDYCDACMKFVQSVEAHIPKISEQ----EFVNLAGNLI--------CNNLKGKSQL------------------------DCKKEVNF----------------FAPLIYRELSMEATPEKICGLVQVCKN--TNAMSFSKIFNSIKFQNDDVCTKCKQVFQFLESMFSKVSP-DEFKFLIESLICNQLPSEVQSTCKLLLDGYVTQLYNLIKSLLDGDRICPLIHLCSSENTQITL-------------------GSWQCQTCLDVIVVLRK-DLAGVSAPEIVSLIKSNVCSKLNFFEKGICNSYVSNLVPTFVNELHSTLTPQQICGNHHVC--TSHSIKKPI-------------ASEKGVIECDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHLKS-ISSQCSSLVDAYADQLITMISNHMDSKAICIAINACKGFSISQRKSAPNFKGIECDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIFQSEKI--QCQQLVDEFFDIILNFVLSELDVDKVCKVMGMCSMKKV--------------------------SGIECEVCERVMDEVIQLAFSNHSQQVVSNFIENKICPRL-GFVKTECTSLIDTYLPVIFSLARSTLNSKVICAELKLCSLISQKKINLLMKSNINDDSAKCKICHFVVSELLTLLTNPDIQKSIENFLENHVCPHFGAK-KQECINDINQYYSLVINIITEFLKP-TLCNELRLCNSTTTSIIMSQPLVKMSRAILSSNCQLCEKFYFHLQKIFLKN 2272
+C GP+YWC+ +NA+ C + C +K I CD C V+ + S EF +A + C+ L +S+L D V F P + ++ + E++C V+ CK + K + + +C C F ++ + S D+ K ++++++C QLPSE + C ++D V QL + S +D C ++ C+ N L G +C C +V+ +R D + +I + IK+++C KL C + V + + L P +C C T + KPI K CDLC++ + S I K +V+ + S +C L + S CS V YA ++ +I +D +C + C S P EC C + + ++ + K I++ LK +C + + +C V ++ +I+++ L + +VC +G+C+ K + +EC VCE +M EV + N S++ + N ++ +C +L + ECT ++ Y ++ +L + K +C L LC + K+ D S +C +C +++SE+ +L +++IEN L+ VC A QEC + +NQY LVIN++ + P T+C L LC ++ K S C LCE + I +N
Sbjct: 29 ECSWGPSYWCSHARNAKACGAVQHCL---DTVWSKQIIKEESQPASCDLCKMVVKETRKMLKSKSRDIKIVEFFTMACQFLPDESLKKECSVLATESELLIKLLKSNIVSKVLSLSFRSLASFDTGSHVRFKSHVTKQTKWPLKTGMLKPRDLKTVAAGRSDEQLCAAVKQCKGFEDKTHQYPKEVPKVSGKVTKICKDCVAFFGDIKQLIQDNSTIDQIKDMLKNVLCTQLPSE-EGLCDFIVDELVPQLLQTLGSEVDPQVFCNMMGFCNGTNINKALVTRIQYRTELKKLNKAKIGGVEECDLCKEVMSKVRDMDRDPTNQEKIKAYIKTDLCEKLGSIADQ-CKTAVDEYADILFELIANELDPTTVCTMLGFCKTTYYVAAKPILPGIQLKPKVNVQEKPKAGELCDLCQMVINELDSMILKNSTTQEVEKALNS-VCKRLPAKYSDDCSDFVKQYAPAILDLIGQELDPLTVCTVLKLCTSESKKLVIQKPVESTTECVLCEMIMKEVDTILQGSKVKEEIESALK-SVCDRLSTGDLAKECSNFVMQYTEILIDL-LKSMPPKEVCSKLGLCTADKAPAKQQLHHVKLVSDKHNSVSHLHSVDASVECVVCEYIMSEVDNILKENRSREAIENALK-MVCSKLPSTISQECTDFVNQYADLVINLLIQEGDPKTVCKALNLCQASA--------KAKSVDASVECVLCEYIMSEVDNILKENRSREAIENALK-MVCSKLPATISQECTDFVNQYADLVINLLIQEGDPKTVCKALNLCQAS----------AKAKSVDASVECVLCEYIMSEVDNILKEN 828 HSP 4 Score: 141.354 bits (355), Expect = 2.787e-30 Identity = 148/684 (21.64%), Postives = 295/684 (43.13%), Query Frame = 2
Query: 251 KNKVDISHH-KNGDYCDACMKFVQSVEAHIPKISEQEFVNLAGNLICNNLKGKSQLDCKKEVNFFAPLIYRELSMEATPEKICGLVQVCKNTNAMSFSKIFNSIKFQNDDVCTKCKQVFQFLESMF--SKVSPDEFKFLIESLICNQLPS-EVQSTCKLLLDGYVTQLYNLIKSLLDGDRICPLIHLCSSENT-------QITL---------------GSWQCQTCLDVIVVLRKDLAGVSAPEIVSLIKSNVCSKLNFFEKGICNSYVSNLVPTFVNELHSTLTPQQICGNHHVCTSHSIKKPIASEKGVIECDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHL-KSISSQCSSLVDAYADQLITMISNHMDSKAICIAINACKGFSISQRKSAPNFKGIECDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIFQSEKIQCQQLVDEFFDIILNFVLSELDVDKVCKVMGMCSMK---------------------KVSGIECEVCERVMDEVIQLAFSNHSQQVVSNFIENKICPRLGFVKTECTSLIDTYLPVIFSLARSTLNSKVICAELKLCSLISQKKINLLMKSNINDDSAKCKICHFVVSELLTLLTNPDIQKSIENFLENHVCPHFGAK-KQECINDINQYYSLVINIITEFLKP-TLCNELRLCNS 2152
K KV++ K G+ CD C + +++ I K S + V A N +C L K DC V +AP I + E P +C ++++C + + K+ ++ C C+ + + ++++ SKV +E + ++S +C++L + ++ C + Y L +L+KS+ +C + LC+++ + L S +C C ++ + L + E + VCSKL C +V+ +N L P+ +C ++C + + K + + +EC LCE ++ + + + + +++ +K +CS L +IS +C+ V+ YAD +I ++ D K +C A+N C+ + ++ A +EC C ++++ + + + +I+N LKM + + +C V+++ D+++N ++ E D VCK + +CS K ++ +CEVCE +++ + + N + Q V + +++C +LP F R L S A L+L KC++C +++ L L +++E L + VC K+EC + QY ++ + ++ L P +C E++LC S
Sbjct: 419 KPKVNVQEKPKAGELCDLCQMVINELDSMILKNSTTQEVEKALNSVCKRLPAKYSDDCSDFVKQYAPAILDLIGQELDPLTVCTVLKLCTSES----KKLVIQKPVESTTECVLCEMIMKEVDTILQGSKVK-EEIESALKS-VCDRLSTGDLAKECSNFVMQYTEILIDLLKSM-PPKEVCSKLGLCTADKAPAKQQLHHVKLVSDKHNSVSHLHSVDASVECVVCEYIMSEVDNILKENRSREAIENALKMVCSKLPSTISQECTDFVNQYADLVINLLIQEGDPKTVCKALNLCQASAKAKSVDAS---VECVLCEYIMSEVDNILKENRSREAIENALKM-VCSKLPATISQECTDFVNQYADLVINLLIQEGDPKTVCKALNLCQASAKAKSVDA----SVECVLCEYIMSEVDNILKENRSREAIENALKMVCSKLPATISQECTDFVNQYADLVINLLIQEGDPKTVCKALNLCSSKQKKTNINSIRFLPIPGQVASLRLGDEKCEVCEVILNYLDEALKENKTVQAVEALL-DQVC---------------NFLPTTFKEERIFLTS---VASLRL-------------------GDEKCEVCEVILNYLDEALKENKTVQAVEALL-DQVCNILPTTFKEECDTIVAQYGPVIAQLFSQLLDPKKICQEIKLCPS 1048 HSP 5 Score: 120.553 bits (301), Expect = 7.807e-24 Identity = 109/444 (24.55%), Postives = 186/444 (41.89%), Query Frame = 2
Query: 1274 LITMISNHMDSKAICIAINACKGFSISQR---KSAPNFKGIE---CDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIFQSEKIQCQQLVDEFFDIILNFVLSELDVDKVCKVMGMC----------------------SMKKVSGI-ECEVCERVMDEVIQLAFSNHSQQVVSNFIENKICPRLGFVKTECTSLIDTYLPVIFSLARSTLNSKVICAELKLCS---LISQKK----INLLMKSNINDDSAK---CKICHFVVSELLTLLTNPDIQKSIENFLENHVCPHFGAK-KQECINDINQYYSLVINIITEFLKP-TLCNELRLCNSTTTSIIMSQPLVKMSRAILSSNCQLCEKFYFHLQKIFLKNTPISPL-TVFANVCN-LEDTDETNECLKHFRNLNSIVAAFHASRNIGEFCKMTEMCKGSKK------HHV 2458
L T+ + D + +C A+ CKGF K P G C C IK+ I D + IK+ LK +C SE+ C +VDE +L + SE+D C +MG C + K+ G+ EC++C+ VM +V + +Q+ + +I+ +C +LG + +C + +D Y ++F L + L+ +C L C ++ K I L K N+ + C +C V++EL +++ + +E L N VC AK +C + + QY ++++I + L P T+C L+LC S + +++ +P+ ++ C LCE + I + + + +VC+ L D EC I+ S E C +C K HHV
Sbjct: 178 LKTVAAGRSDEQ-LCAAVKQCKGFEDKTHQYPKEVPKVSGKVTKICKDCVAFFGDIKQLIQDNSTIDQIKDMLKNVLCTQLPSEEGLCDFIVDELVPQLLQTLGSEVDPQVFCNMMGFCNGTNINKALVTRIQYRTELKKLNKAKIGGVEECDLCKEVMSKVRDMDRDPTNQEKIKAYIKTDLCEKLGSIADQCKTAVDEYADILFELIANELDPTTVCTMLGFCKTTYYVAAKPILPGIQLKPKVNVQEKPKAGELCDLCQMVINELDSMILKNSTTQEVEKAL-NSVCKRLPAKYSDDCSDFVKQYAPAILDLIGQELDPLTVCTVLKLCTSESKKLVIQKPVES------TTECVLCEMIMKEVDTILQGSKVKEEIESALKSVCDRLSTGDLAKECSNFVMQYTEILIDLLKSMPPKEVCSKLGLCTADKAPAKQQLHHV 613
BLAST of Proactivator polypeptide vs. TrEMBL
Match: A0A2G8K9Z3 (Uncharacterized protein OS=Stichopus japonicus OX=307972 GN=BSL78_18317 PE=4 SV=1) HSP 1 Score: 185.267 bits (469), Expect = 6.777e-44 Identity = 167/710 (23.52%), Postives = 288/710 (40.56%), Query Frame = 2
Query: 281 NGDYCDACMKFVQSVEAHIPKISEQEFVNLAGNLICNNLKGKSQLDCKKEVNFFAPLIYRELSMEATPEKICGLVQVCKNTNAMSFSKIFNSIKFQNDDVCTKCKQVFQFLESMFSKVSPDEFKFLIESLICNQLPSEVQSTCKLLLDGYVTQLYNLIKSLLDGDRICPLIHLCS--SENTQI-TLGSWQCQTCLDVIVVLRKDLAGVSAPEIVSLIKSNVCSKLNFFEKGICNSYVSNLVPTFVNELHSTLTPQQICGNHHVCTSHSIKKPIASEKGVIECDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHL-KSISSQCSSLVDAYADQLITMISNHMDSKAICIAINACKG-------------FSISQRKSAP-NFKGIECDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIFQSEKI-QCQQLVDEFFDIILNFVLSELDVDKVCKVMGMCSMKKVSGIECEVCERVMDEVIQLAFSNHSQQVVSNFIENKICPRL-GFVKTECTSLIDTYLPVIFSLARSTLNSKVICAELKLCSLISQKKINLLMKSNINDDSAKCKICHFVVSELLTLLTNPDIQKSIENFLENHVCPHFGAKKQECINDINQYYSLVINIITEFLKPTL-CNELRLCNSTTTSIIMSQPLVKMSRAILSSNCQLCEKFYFHLQKIFLKNTPISPL-TVFANVCNLEDTDETNEC 2344
NGD C C F Q V + S Q+ + ++CN L C++ V + PL++ L P ICG + C ++ + N D CT CK FQ + +M S + +CN L + C+ + Y ++N + LD IC I C SE T++ L + QC C + + L S +++ N+C+ L C YVS +N L L Q C C+ + ++ P ++ +++ +C+ L SI QC V Y +I + ++D ++C I C G +S P +G C+ C L V++I+ Y+ D A + I L+ + C I S QC +D++ ++L + E+D D+VC + +C+ + +C +CE VM ++ + + + +E K+C L G + +C +D Y P I +L ++ ++IC L LC S+ + S KC +C +V+ +L T+++ IE LE G+ +C ++QY +IN+I + + P L C L LC +++ S + Q L C LC+ ++ + K + IS + VC+L + +C
Sbjct: 899 NGDQCTDCKNFFQDVATLLKNSSVQQMILQEIGMLCNELGPSIGPQCQQYVTEYGPLVFNMLLQYLDPTAICGDIGFCTAVSSPPPLHL-------NGDQCTDCKNFFQDVSTMLKNTSTQQMILQELETLCNNLGPSIGPQCQQYVSEYGPLVFNFLLQYLDPTAICGDIGFCPAMSEPTKLFQLNADQCTDCKNFFQDVSTILNNPSTQQMIIEDIENLCNSLGPSLGPQCQQYVSEYGSLVINFLLQYLNADQ-------------------------CTDCKNFFQDVVTILNNPSTQQQILQELET-LCNDLGPSIGPQCQQYVSEYGPLVINFLLQYLDPASVCSQIGFCSGMNEVISFVELQSALPVSPMNEEPLEEEGAGCEVCKLVVSQIEVYLKDGATEEEITAALE-KFCSILPSSLANQCTAFMDQYGPVVLKLLADEMDPDQVCSFLKLCAAEFQQSPKCTLCEYVMQKLETMISKQSTVAEIEAALE-KVCSLLPGSISDQCQQFVDQYTPTIINLIVQNVSPQLICRTLGLCKASSESEFQ---------QSPKCTLCEYVIQKLETMISKQSTVAEIEAALEKVCSLLPGSISDQCQQFVDQYTPTIINLIVQNVSPQLICRTLGLCKASSESDVHEQ---------LGPTCVLCQYVMKEVESLLAKESTISDIEAALDKVCSLMPGTLSQQC 1555 HSP 2 Score: 167.548 bits (423), Expect = 2.394e-38 Identity = 152/656 (23.17%), Postives = 286/656 (43.60%), Query Frame = 2
Query: 275 HKNGDYCDACMKFVQSVEAHIPKISEQEFVNLAGNLICNNLKGKSQLDCKKEVNFFAPLIYRELSMEATPEKICGLVQVCKNTNAMS-FSKIFNSIKFQNDDVCTKCKQVFQFLESMFSKVSPDEFKFLIESL--ICNQLPSEVQSTCKLLLDGYVTQLYNLIKSLLDGDRICPLIHLCSSENTQITLGSWQCQTC----LDVIVVLRKDLAGVSAPEIVSLIKSNVCSKLNFFEKGICNSYVSNLVPTFVNELHSTLTPQQICGNHHVCT-----------------SHSIKKPIASEKGVIECDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHL-KSISSQCSSLVDAYADQLITMISNHMDSKAICIAINACKGFSISQRKSAPNFKGIECDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIFQ-SEKIQCQQLVDEFFDIILNFVLSELDVDKVCKVMGMCSMKKVSGIE----CEVCERVMDEVIQLAFSNHSQQVVSNFIENKICPRL-GFVKTECTSLIDTYLPVIFSLARSTLNSKVICAELKLCSLISQKKINLLMKSNINDDSAKCKICHFVVSELLTLLTNPDIQKSIENFLENHVCPHFGAKKQECINDINQYYSLVINIITEFLKPTL-CNELRLC 2146
H NGD C C F Q V + S Q+ + +CNNL C++ V+ + PL++ L P ICG + C AMS +K+F N D CT CK FQ + ++ + +P + +IE + +CN L + C+ + Y + + N + L+ D QC C DV+ +L + +I+ +++ +C+ L C YVS P +N L L P +C C+ S ++P+ E C++C++ V+ + ++ ++ + ++ K CS L S+++QC++ +D Y ++ ++++ MD +C + C ++ + +P +C C + K++ IS + I+ L+ ++C + S QCQQ VD++ I+N ++ + +C+ +G+C S + C +CE V+ ++ + + + +E K+C L G + +C +D Y P I +L ++ ++IC L LC S+ ++ + C +C +V+ E+ +LL IE L+ G Q+C ++ Y ++N++ +++ P L C LRLC
Sbjct: 986 HLNGDQCTDCKNFFQDVSTMLKNTSTQQMILQELETLCNNLGPSIGPQCQQYVSEYGPLVFNFLLQYLDPTAICGDIGFCP---AMSEPTKLFQL----NADQCTDCKNFFQDVSTILN--NPSTQQMIIEDIENLCNSLGPSLGPQCQQYVSEYGSLVINFLLQYLNAD---------------------QCTDCKNFFQDVVTILNNP---STQQQILQELET-LCNDLGPSIGPQCQQYVSEYGPLVINFLLQYLDPASVCSQIGFCSGMNEVISFVELQSALPVSPMNEEPLEEEGA--GCEVCKLVVSQIEVYLKDGATEEEITAALE-KFCSILPSSLANQCTAFMDQYGPVVLKLLADEMDPDQVCSFLKLCA----AEFQQSP-----KCTLCEYVMQKLETMISKQSTVAEIEAALE-KVCSLLPGSISDQCQQFVDQYTPTIINLIVQNVSPQLICRTLGLCKASSESEFQQSPKCTLCEYVIQKLETMISKQSTVAEIEAALE-KVCSLLPGSISDQCQQFVDQYTPTIINLIVQNVSPQLICRTLGLCKASSESDVHEQL-------GPTCVLCQYVMKEVESLLAKESTISDIEAALDKVCSLMPGTLSQQCQQFVDTYTPTIVNLLVQYVPPRLVCVVLRLC 1586 HSP 3 Score: 163.696 bits (413), Expect = 3.717e-37 Identity = 170/763 (22.28%), Postives = 299/763 (39.19%), Query Frame = 2
Query: 284 GDYCDACMKFVQSVEAHIPKISEQEFVNLAGNLICNNLKGKSQLDCKKEVNFFAPLIYRELSMEATPEKICGLVQVCKNTNAMSFSKIFNSIKFQNDDVCTKCKQVFQFLESMFSKVSPDEFKFLIESLICNQLPSEVQSTCKLLLDGYVTQLYNLIKSLLDGDRICPLIHLCS--SENTQITLGSWQCQTCLDVIVVLRKDLAGVSAPEIVSLIKSNVCSKLNFFEKGICNSYVSNLVPTFVNELHSTLTPQQICGNHHVCTSHSIKKPIASEKGVIECDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHLKSISSQCSSLVDAYADQLITMISNHMDSKAICIAINACKGFSISQRKSAPNFKGIECDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIFQSEKIQCQQLVDEFFDIILNFVLSELDVDKVCKVMGMCS-MKKV----------------------SGIECEVCERVMDEVIQLAFSNHSQQVVSNFIENKICPRL-GFVKTECTSLIDTYLPVIFSLARSTLNSKVICAELKLCSLISQKKINLLMKSNINDDSAKCKICHFVVSELLTLLTNPDIQKSIENFLENHVCPHFGAKKQECINDINQYYSLVINIITEFLKPTL-CNELRLCNSTTTSIIMSQPLVKMSRAILSSNCQLCEKFYFHLQKIFLKNTPISPL-TVFANVCNLEDTDETNECLKHFRNLN-SIVAAFHASRNIGEFCKMTEMCKGSKKHHVGKKQCSWGP 2485
D C C F Q V + S Q+ + A +CN+L C++ V+ + PL L P ICG + C + K S+ N D CT CK FQ + ++ S + ++CN+L + C+ + Y ++N++ LD IC I C+ S + L QC C + + L S +++ +C+ L C YVS P N L L P ICG+ C + S + + +C C+ + ++ P + I++ S S+ QC V Y +I + ++++ +C C + +++P+ + I L+ + S QCQQ V E+ +++NF+L LD VC +G CS M +V G CEVC+ V+ ++ +++ ++ +E K C L + +CT+ +D Y PV+ L ++ +C+ LKLC+ Q+ S KC +C +V+ +L T+++ IE LE G+ +C ++QY +IN+I + + P L C L LC +++ S P C LCE L+ + K + ++ + VC+L +++C + +I+ + + C+ +CK S + V ++ GP
Sbjct: 809 ADQCADCKNFFQDVVTILNNPSTQQQILQAIETLCNDLGPSIGPQCQQYVSEYGPLAINFLLQYLDPNMICGEIGFCA-----ALPKQSMSLPSLNGDQCTDCKNFFQDVATLLKNSSVQQMILQEIGMLCNELGPSIGPQCQQYVTEYGPLVFNMLLQYLDPTAICGDIGFCTAVSSPPPLHLNGDQCTDCKNFFQDVSTMLKNTSTQQMILQELETLCNNLGPSIGPQCQQYVSEYGPLVFNFLLQYLDPTAICGDIGFCPAMSEPTKLF-QLNADQCTDCKNFFQDVSTILNNPSTQQMIIEDIENLCNSLGPSLGPQCQQYVSEYGSLVINFLLQYLNAD--------------------------QCTDCKNFFQDVVTILNNPSTQQQILQELETLCNDLGPSIGPQCQQYVSEYGPLVINFLLQYLDPASVCSQIGFCSGMNEVISFVELQSALPVSPMNEEPLEEEGAGCEVCKLVVSQIEVYLKDGATEEEITAALE-KFCSILPSSLANQCTAFMDQYGPVVLKLLADEMDPDQVCSFLKLCAAEFQQ-------------SPKCTLCEYVMQKLETMISKQSTVAEIEAALEKVCSLLPGSISDQCQQFVDQYTPTIINLIVQNVSPQLICRTLGLCKASSESEFQQSP-----------KCTLCEYVIQKLETMISKQSTVAEIEAALEKVCSLLPGSISDQCQQFVDQYTPTIINLIVQNVSPQLICRTLGLCKASSESDVHEQ---LGP 1511 HSP 4 Score: 150.214 bits (378), Expect = 6.252e-33 Identity = 170/792 (21.46%), Postives = 316/792 (39.90%), Query Frame = 2
Query: 269 SHHKNGDYCDACMKFVQSVEAHIPKISEQEFVNLAGNLICNNLKGKSQLDCKKEVNFFAPLIYRELSMEATPEKICGLVQVCKNTNAMSFSKIFNSIKFQNDDVCTKCKQVFQFLESMFSKVSP-DEFKFLIESLICNQLPSEVQSTCKLLLDGYVTQLYNLIKSLLDGDRICPLIHLC---SSENTQITLGSWQCQTCLDVIVVLRKDLAGVSAPEIVSLIKSNVCSKLNFFEKGICNSYVSNLVPTFVNELHSTLTPQQICGNHHVCTSHSIKKPIASEKGVIECDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHLKSISSQCSSLVDAYADQLITMISNHMDSKAICIAINACKGFS----ISQRKSAPNFK------GIECDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIFQSE-KIQCQQLVDEFFDIILNFVLSELDVDKVCKVMGMCS-----MKKVSGIECEVCERVMD-EVIQLAFSNHSQQVVSNFIENKICPRL-GFVKTECTSLIDTYLPVIFSLARSTLNSKVICAELKLCSLISQKKINLLMKSNINDDSAKCKICHFVVSELLTLLTNPDIQKSIENFLENHVCPHFGAK-KQECINDINQYYSLVINIITEFLKPT-LCNELRLCNSTTTSIIMSQPLVKMSRAILSSNCQLCEKFYFHLQKIFLKNTPISPLTVFANVCNLEDTDETNECLKHFRNL-NSIVAAFHASRNIGEFCKMTEMC-KGSKKHHVGKKQCSWGPGFWCISRQHAAGCGPGAVKHCEMKVWN 2566
+ + C C +Q +E I K S + A +C+ L G C++ V+ + P I + +P+ IC + +CK ++ +FQ CT C+ V Q LE+M SK S E + +E +C+ LP + C+ +D Y + NLI + IC + LC S + LG C C V+ + LA S + VCS + C +V PT VN L + P+ +C +C P+ +E C +CE ++ + +P Y ++I +I+ + K +C A+ C +SQ ++ + EC C A+ ++++ + A + I+ F +C + + C L++ + ++ + + +K+C ++ +C+ +K V+ +E E R + ++ +++ S++++ N +C RL V +C +D Y I + S +C + LC+ K + + ++ C IC FV L L + I+ L + VC + +EC ++ Y +I I+ P+ LC+ L+LC +++ QP + ++ +C++C + KNT E ++C+ + N+I + + CK+ ++C + + ++C GP FWC S +A C AV+HC+ VWN
Sbjct: 1329 AEFQQSPKCTLCEYVMQKLETMISKQSTVAEIEAALEKVCSLLPGSISDQCQQFVDQYTPTIINLIVQNVSPQLICRTLGLCKASSES---------EFQQSPKCTLCEYVIQKLETMISKQSTVAEIEAALEK-VCSLLPGSISDQCQQFVDQYTPTIINLIVQNVSPQLICRTLGLCKASSESDVHEQLGPT-CVLCQYVMKEVESLLAKESTISDIEAALDKVCSLMPGTLSQQCQQFVDTYTPTIVNLLVQYVPPRLVCVVLRLCMFEEAPAPVLNEGANEFCAICEFAMSELDKILVEPNT----------------------------EYTKEIIFLITQSVSPKQVCAALTLCTSVDSADEVSQDETLDHIDEQKFEASTECIVCEYAMKELEKQLGKNATEQEIR-FALDEVCSLLPGTLQEDCSILINYYTTEVIYQFIRKYPANKICTLLKVCAAVDGELKSVAHVEVERSLRSLSVNLLSRSWNRCSRRILQRKALNSLCSRLPSTVAKDCQMFVDYYTEEIIFILVSQFPPSSLCTSINLCTASEIKGVT----EKVEQNTQFCTICEFVAKYLEEDLQKNSTEAEIKKAL-DLVCSKLPSTVTKECQMFVDYYTEEIIFILVSQFPPSKLCSSLQLC----SAVEEIQPQIDEDTSV---SCEVCVIAVRSAELYITKNT---------------TEQEIDKCVTFVQQFGNAIPELIVTFGDPDKICKLLDLCPQNVDVKQLMYEKCKTGPEFWCASETNADLC--DAVEHCKRHVWN 2051 HSP 5 Score: 132.494 bits (332), Expect = 1.990e-27 Identity = 149/724 (20.58%), Postives = 292/724 (40.33%), Query Frame = 2
Query: 158 CLKGPTYWCATYQNAEKCDRITECFMGQGDQKNKVDISHHKNGDYCDACMKFVQSVEAHIPKISEQEFVNLAGNLICNNLKGKSQLDCKKEVNFFAPLIYRELSMEATPEKICGLVQVCKNTNAMSFSKIFNSIKFQNDDVCTKCKQVFQFLESMFSKVSPDEFKFLIESLICNQLPSEVQSTCKLLLDGYVTQLYNLIKSLLDGDRICPLIHLCSSENTQITLGSWQCQTCLDVIVVLRKDLAGVSAPEIVSLIKSNVCSKLNFFEKGICNSYVSNLVPTFVNELHSTLTPQQICGNHHVCTSHSIKKPIASEKGVIECDLCEIGVTGAKSFISKPENMNKVKSFIKS--KICSHLKSISSQCSSLVDAYADQLITMISNHMDSKAICIAINACKGFSISQRKSAPNFKGIECDACHLAVTKIKEYISDPARKTSIKNFLKM--RICPIFQSEKIQCQQLVDEFFDIILNFVLSELDVDKVCKVMGMCSMKKV------SGIECEVCERVMDEVIQLAFSNHSQQVVSNFIENKICPRLGFVKTECTSLIDTYLPVIFSLARSTLNSKVICAELKLCSLISQKKINL------LMKSNINDDSAKCKICHFVVSELLTLLTNPDIQKSIENFLENHVCPHFGAKKQECINDINQYYSLVINIITEFLKP-TLCNELRLCNSTTTSIIMSQPLVKMSRAILSSNCQLCEKFYFHLQKIFLKNTP 2278
C GPTYWC + + A++C + C N K G++C+ C V + + K S ++ + C +L ++ DC+ VN + P + + L + P+++C L+ CK +++ + S + + Q ++ SK LL+ TQL+ GD++C C + I ++K L + +++ +C L + K C V+ LVP + +L + +Q C C S +P+ C C+ + K+ +S N + K I+ C+ L S++ QC V+ Y + ++I + D IC +I CK S+ +F C C + ++++ P+ + +N +K IC + + C Q+V E+ +I+ +L L+ + CK +G+C+ + + G C C+ + + L + Q+++ E +C + ++C I Y ++F + +N K +C ++ LC+ + + L ++K+ +D C C + ++ L+ PD+QK I +E +C G +C ++QY LV + +++ P +C+ + LC++ L + + + C C+ F+ + I NTP
Sbjct: 23 CSHGPTYWCGSIKQAKECGAVQYC------SDNDWAAIRTKAGEFCNDCEIGVGLLTEQLEKASTEDEIIQIVEKFCTDLGALAE-DCRLVVNAYGPEMLKALIEKLQPDQVCALLGFCKPDTSVTDPQEDQSAQI-----------MLQLMDRASSK----------------------------LLEILGTQLFA-------GDKMC--------------------TDCTNFIGDVKKLLNNATVEAMITGEVKRLCQYLGAY-KTECELIVTTLVPQGIGQLVEKINAKQDCSQIGFCKSLHSDEPVPEPPTNDPCSDCKAFMGDVKTILS---NSSVQKMIIEDLENFCNDLGSLAPQCQEYVEQYGSLVFSLILQYFDPNMICSSIGFCK----SEEMVMASFD--VCTECKTYMGDFVQFLNTPSVQ---QNVMKQVTSICKMLKISD-NCDQVVSEYGPLIMKMLLQYLNPESFCKEIGVCASEAIPEALPEPGDLCTDCQSFVKDFDNLLNNYTVQEMLLKDFE-VLCKYVPNYSSDCKMYIKQYGALVFEVIAGLINPKTVCTDIGLCNGVRSPRPTLPKLPVEVLKALAPNDV--CADCKNLFQDVQNFLSEPDVQKMILGKIE-LLCNDLGPFGPQCKTYVDQYGPLVFTFLLQYIDPDAICSNVGLCSAD---------LPQSPAKVNADMCADCKAFFTDVSTIL--NTP 644 HSP 6 Score: 128.642 bits (322), Expect = 3.048e-26 Identity = 151/710 (21.27%), Postives = 277/710 (39.01%), Query Frame = 2
Query: 266 ISHHKNGDYCDACMKFVQSVEAHIPKISEQEFVNLAGNLICNNLKGKSQLDCKKEVNFFAPLIYRELSMEATPEKICGLVQVCKNTNAMSFSKIFNSIKFQNDDVCTKCKQVFQFLESMFSKVSPDEFKFLIESLICNQLPSEVQSTCKLLLDGYVTQLYNLIKSLLDGDRICPLIHLCSSENTQITLGSWQ--CQTCLDVIVVLRKDLAGVSAPEIVSLIKSNVCSKLNFFEKGICNSYVSNLVPTFVNELHSTLTPQQICGNHHVCTSHSIKKPIASEKGV---------IECDLCEIGVTGAKSFISKPENMNKVKSFIKSKI---CSHLKSISSQCSSLVDAYADQLITMISNHMDSKAICIAINACKGFSISQRKSAPNFKGIECDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIFQSEKIQCQQLVDEFFDIILNFVLSELDVDKVCKVMGMCS------------------MKKVSGIECEVCERVMDEVIQLAFSNHSQQVVSNFIENKICPRLGFVKTECTSLIDTYLPVIFSLARSTLNSKVICAELKLCSLISQKKINLLMKSNINDDSAKCKICHFVVSELLTLLTNPDIQKSIENFLE---NHVCPHFGAKKQECINDINQYYSLVINIITEFLKPTL-CNELRLCNSTTTSIIMSQPLVKMSRAILSSNCQLCE--KFYFHLQKIFLKNTPI 2281
+ D C C F+ V+ + S Q+ + CN+L G C++ V + L++ + P IC + CK+ + S DVCT+CK + S + + IC L ++ C ++ Y + ++ L+ + C I +C+SE L C C + L + E++ +C + + C Y+ + + P+ +C + +C +P + V C C+ ++F+S+P+ V+ I KI C+ L QC + VD Y + T + ++D AIC + C S +S C C T + ++ P + I ++ + S QCQQ V E+ +++NFVL LD +C +G C+ M V+G C C+ ++++ L + Q++ N + +C G +++EC LI Y P++F + ++ IC ++ LC ++ + +K++ +C C +++T+L NP Q+ I +E N + P G +C +++Y L IN + ++L P + C E+ C + L K S ++ S N C K +F LKN+ +
Sbjct: 259 VPEPPTNDPCSDCKAFMGDVKTILSNSSVQKMIIEDLENFCNDL-GSLAPQCQEYVEQYGSLVFSLILQYFDPNMICSSIGFCKSEEMVMASF----------DVCTECKTYMGDFVQFLNTPSVQQNVMKQVTSICKML--KISDNCDQVVSEYGPLIMKMLLQYLNPESFCKEIGVCASEAIPEALPEPGDLCTDCQSFVKDFDNLLNNYTVQEMLLKDFEVLCKYVPNYSSD-CKMYIKQYGALVFEVIAGLINPKTVCTDIGLCNGVRSPRPTLPKLPVEVLKALAPNDVCADCKNLFQDVQNFLSEPD----VQKMILGKIELLCNDLGPFGPQCKTYVDQYGPLVFTFLLQYIDPDAICSNVGLC---SADLPQSPAKVNADMCADCKAFFTDVSTILNTPQVQQMILQEIESLCNDLGPSISPQCQQYVTEYGPLVINFVLQYLDPTSICGDIGFCTGVNTVRPLAFTAVKTILKMPTVAGDLCTDCKNLVNDADGLLKNATVQKMFLNDLA-VVCKYFGGLQSECKDLIMEYGPLLFDYIAAYIDPTTICDDIGLCPAVT-----VTLKAD------QCADCKNFFQDVVTILNNPSTQQQILQAIETLCNDLGPSIGP---QCQQYVSEYGPLAINFLLQYLDPNMICGEIGFCAA----------LPKQSMSLPSLNGDQCTDCKNFFQDVATLLKNSSV 922 HSP 7 Score: 127.872 bits (320), Expect = 5.358e-26 Identity = 129/546 (23.63%), Postives = 241/546 (44.14%), Query Frame = 2
Query: 275 HKNGDYCDACMKFVQSVEAHIPKISEQEFVNLAGNLICNNLKGKSQLDCKKEVNFFAPLIYRELSMEATPEKICGLVQVCKNTNAMSFSKIFNSIKFQNDDVCTKCKQVFQFLESMFSKVSP-DEFKFLIESLICNQLPSEVQSTCKLLLDGYVTQLYNLIKSLLDGDRICPLIHLCSSENTQITLGSWQCQTCLDVIVVLRKDLAGVSAPEIVSLIKSNVCSKLNFFEKGICNSYVSNLVPTFVNELHSTLTPQQICGNHHVCTSHSIKKPIASEKGVIECDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHLK-SISSQCSSLVDAYADQLITMISNHMDSKAICIAINACKGFSISQRKSAPNF-KGIE--CDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIFQ--SEKIQCQQL-----VDEFFDIILNFVLSELDVDKVCKVMGMCSMKKVSGIECEVCERVMDEVIQLAFSNHSQQVVSNFIENKICPRL-GFVKTECTSLIDTYLP-VIFSLARSTLNSKVICAELKLCSLI 1870
+ G C+ C V +E ++ + +E + A C+ L C ++ + P++ + L+ E P+++C +++C + +FQ CT C+ V Q LE+M SK S E + +E +C+ LP + C+ +D Y + NLI + IC + LC + + S +C C VI L ++ S + VCS L C +V PT +N + ++PQ IC +C + S + + + G C LC+ + +S ++K ++ +++ + K+CS + ++S QC VD Y ++ ++ ++ + +C+ + C + + AP +G C C A++++ + + +P + + K I I Q S K C L VD ++ + L +D K + EC VCE M E+ + N ++Q + F +++C L G ++ +C+ LI+ Y VI+ R +K IC LK+C+ +
Sbjct: 1248 EEEGAGCEVCKLVVSQIEVYLKDGATEEEITAALEKFCSILPSSLANQCTAFMDQYGPVVLKLLADEMDPDQVCSFLKLC-------------AAEFQQSPKCTLCEYVMQKLETMISKQSTVAEIEAALEK-VCSLLPGSISDQCQQFVDQYTPTIINLIVQNVSPQLICRTLGLCKASSESEFQQSPKCTLCEYVIQKLETMISKQSTVAEIEAALEKVCSLLPGSISDQCQQFVDQYTPTIINLIVQNVSPQLICRTLGLCKASS-ESDVHEQLGPT-CVLCQYVMKEVESLLAKESTISDIEAAL-DKVCSLMPGTLSQQCQQFVDTYTPTIVNLLVQYVPPRLVCVVLRLC----MFEEAPAPVLNEGANEFCAICEFAMSELDKILVEPNTE-----YTKEIIFLITQSVSPKQVCAALTLCTSVDSADEVSQDETLDHID-----------EQKFEASTECIVCEYAMKELEKQLGKNATEQEI-RFALDEVCSLLPGTLQEDCSILINYYTTEVIYQFIRKYPANK-ICTLLKVCAAV 1754
BLAST of Proactivator polypeptide vs. TrEMBL
Match: A0A2T7PW46 (Uncharacterized protein OS=Pomacea canaliculata OX=400727 GN=C0Q70_00216 PE=4 SV=1) HSP 1 Score: 176.022 bits (445), Expect = 5.433e-41 Identity = 183/832 (22.00%), Postives = 337/832 (40.50%), Query Frame = 2
Query: 293 CDACMKFVQSVEAHIPKISEQEFVNLAGNLICNNLKGKSQLDCKKEVNFFAPLIYRELSMEATPEKICGLVQVCKNTNAMSFSKIFNSI---KFQNDDVCTKCKQVFQFLESMFSK-VSPDEFKFLIESLICNQLPSEVQSTCKLLLDGYVTQLYNLIKSLLDGDRICPLIHLCSSENTQ------ITLGSWQ------CQTCLDVIVVLRKDLAGVSAPEIVSLIKSNVCSKLNFFEKGICNSYVSNLVPTFVNELHSTLTPQQICGNHHVC------------TSHSIKKPIASEKGVIECDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHL-KSISSQCSSLVDAYADQLITMISNHMDSKAICIAINACKGFSISQRKSAPNFKGIE---------------------------------------------CDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIFQSE-KIQCQQLVDEFFDIILNFVLSELDVDKVCKVMGMCSMK-KVSGIECEVCERVMDEVIQLAFSNHSQQVVSNFIENKICPRL--GFVKTECTSLIDTYLPVIFSLARSTLNSKVICAELKLCSLISQKKINLLMKSNINDDSAKCKICHFVVSELLTLLTNPDIQKSIENFLENHVCPHFGAK-KQECINDINQYYSLVINIITEFLKPT-LCNELRLCNSTTTSIIMSQPLVKMSRAILSSN-CQLCEKFYFHLQKIFLKNTPISPLTVFAN-VCNLEDTDETNECLKHFRNLNSIVAAFHASR-NIGEFCKMTEMCKG----SKKHHVGKKQCSWGPGFWCISRQHAAGCG 2527
C+ C + +Q +E + + +E V A +C+ +C++ V ++ + L + IC + +C +T ++ ++ + K +C CKQV Q L+S+ K + D+ + ++ +C P+ + S C+ L+ Y Q+ I L + IC L+ LCS N T+G + C C +V+ + L + + VC C V+ P + + L P++IC +C T+ +KP A G I CDLC+ + S + K ++ ++ + ++C +ISSQC LV YA Q+I I+ + + IC + C + Q K++ + E CD C + K+ + I+ L ++C +F + QC+QLV ++ +++ ++ EL D++C ++ +CS K +G C++C++V+ + L N + + + +++C RL + ++C L+ Y P I L + IC+ LKLCS +K C +C V+ + +LL IE L + VC F A +C + QY +I I E LKP +C+ L LC+ + + + + A+ C +C+ ++++ + + + N VC L TD T+EC + ++ A N C+ ++C S +G C++GP +WC ++A CG
Sbjct: 780 CELCNQVMQLIETFLQQNRTEETVTKALQQVCSFFPDTISTECRQFVGRYSQEVINLLLQDLNATDICKKLGLCSDTRPVTGKELTTTAGDKKPSAGPICDLCKQVLQTLDSLLEKNRTVDDIEKALDR-VCKLFPATISSQCRQLVGQYAPQIIEYIAEELKPEEICSLLGLCSQGNGAQETKPVATIGEEKPSAGPICDLCKEVLQTVDSLLEKNRTVDDIEKALDQVCRLFPATISSQCRQLVAQYAPQIIEYIADELKPEEICSLLGLCSQGKKVQGKELTTATDEQKPAA---GPI-CDLCKEVLQAVDSLLEKNRTVDDIEKAL-DQVCRLFPATISSQCRQLVGQYAPQIIEYITEELKPEEICSLLGLCSQGNRDQGKASDGGRQTEAISWSHLRVVQSGLDNTGQSGREEQHSGQYHEAAVGELKPSAGPICDLCKEVLQKVDTLLEKNRTVDDIEKALD-QVCRLFPASIASQCRQLVAQYAPALIDLIVEELQPDEICSLLKLCSQSNKAAGPICDLCKQVLQTLDSLLEKNRTVDDIEKAL-DQVC-RLFPATIASQCRQLVGQYAPQIIEYIADELKPEEICSLLKLCSQGKKKPAA----------GPICDLCKEVLQAVDSLLEKNRTVDDIEKAL-DQVCRLFPATISSQCRQLVGQYAPQIIEYIAEELKPEEICSLLGLCSQGHNE--LGREVETQAVAVREGELCLICDTLLNYVEEGLNQQATVEEVEKLLNDVCKLFPTDLTSECSLLVQQFTPLIMQLLAEEVNPKAICETIKLCPSPNSTSHPKFLGLDPCTFGPAYWCAKLENAEECG 1589 HSP 2 Score: 146.747 bits (369), Expect = 6.894e-32 Identity = 151/699 (21.60%), Postives = 289/699 (41.34%), Query Frame = 2
Query: 284 GDYCDACMKFVQSVEAHIPKISEQEFVNLAGNLICNNLKGKSQLDCKKEVNFFAPLIYRELSMEATPEKICGLVQVCKNTNAMSFSKIFNSI---KFQNDDVCTKCKQVFQFLESMFSK-VSPDEFKFLIESLICNQLPSEVQSTCKLLLDGYVTQLYNLIKSLLDGDRICPLIHLCSS----ENTQITLGSWQ--------CQTCLDVIVVLRKDLAGVSAPEIVSLIKSNVCSKLNFFEKGICNSYVSNLVPTFVNELHSTLTPQQICGNHHVCTSHSIKKPIASEKG------------VIE----------------------------------CDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHL-KSISSQCSSLVDAYADQLITMISNHMDSKAICIAINACKGFSISQRKSAPNFKGIECDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIFQSE-KIQCQQLVDEFFDIILNFVLSELDVDKVCKVMGMCSM---KKVSGIECEVCERVMDEVIQLAFSNHSQQVVSNFIENKICPRL--GFVKTECTSLIDTYLPVIFSLARSTLNSKVICAELKLCSLI-SQKKINLLMKSNINDDSAKCKICHFVVSELLTLLTNPDIQKSIENFLENHVCPHFGAK-KQECINDINQYYSLVINIITEFLKP-TLCNELRLCNSTTTS 2164
G CD C + +Q++++ + K + + A + +C C++ V +AP I ++ E PE+IC L+ +C N +K +I K +C CK+V Q ++S+ K + D+ + ++ +C P+ + S C+ L+ Y Q+ I L + IC L+ LCS + ++T + + C C +V+ + L + + VC C V P + + L P++IC +C+ + + AS+ G V++ CDLC+ + + + K ++ ++ + ++C SI+SQC LV YA LI +I + IC + C S S + + P CD C + + + I+ L ++C +F + QC+QLV ++ I+ ++ EL +++C ++ +CS K +G C++C+ V+ V L N + + + +++C RL + ++C L+ Y P I L + IC+ L LCS ++ + ++ + C IC +++ + L + +E L N VC F EC + Q+ L++ ++ E + P +C ++LC S ++
Sbjct: 876 GPICDLCKQVLQTLDSLLEKNRTVDDIEKALDRVCKLFPATISSQCRQLVGQYAPQIIEYIAEELKPEEICSLLGLCSQGNGAQETKPVATIGEEKPSAGPICDLCKEVLQTVDSLLEKNRTVDDIEKALDQ-VCRLFPATISSQCRQLVAQYAPQIIEYIADELKPEEICSLLGLCSQGKKVQGKELTTATDEQKPAAGPICDLCKEVLQAVDSLLEKNRTVDDIEKALDQVCRLFPATISSQCRQLVGQYAPQIIEYITEELKPEEICSLLGLCSQGNRDQGKASDGGRQTEAISWSHLRVVQSGLDNTGQSGREEQHSGQYHEAAVGELKPSAGPICDLCKEVLQKVDTLLEKNRTVDDIEKAL-DQVCRLFPASIASQCRQLVAQYAPALIDLIVEELQPDEICSLLKLC---SQSNKAAGPI-----CDLCKQVLQTLDSLLEKNRTVDDIEKALD-QVCRLFPATIASQCRQLVGQYAPQIIEYIADELKPEEICSLLKLCSQGKKKPAAGPICDLCKEVLQAVDSLLEKNRTVDDIEKAL-DQVC-RLFPATISSQCRQLVGQYAPQIIEYIAEELKPEEICSLLGLCSQGHNELGREVETQAVAVREGELCLICDTLLNYVEEGLNQQATVEEVEKLL-NDVCKLFPTDLTSECSLLVQQFTPLIMQLLAEEVNPKAICETIKLCPSPNST 1560 HSP 3 Score: 144.436 bits (363), Expect = 3.959e-31 Identity = 186/824 (22.57%), Postives = 328/824 (39.81%), Query Frame = 2
Query: 293 CDACMKFVQSVEAHIPKISEQEFVNLAGNLICNNLKGKSQLDCKKEVNFFAPLIYRELSMEATPEKICGLVQVCKNTNAMSFSKIFNSIKFQNDDV----CTKCKQVFQFLESMFSKVSPDEFKFLIESLICNQLPSEVQSTCKLLLDGYVTQLYNLIKSLLDGDRICPLIHLCS---------SENTQITLGSWQCQTCLDVIVVLRKDLAGVSAPEIVSLIKSNVCSKLNFFEKGI---CNSYVSNLVPTFVNELHSTLTPQQICGNHHVC------------TSHSIKKPIASEKGVIECDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHL-KSISSQCSSLVDAYADQLITMISNHMDSKAICIAINACKGFSISQRKSAPNFKGIE-------CDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIFQSE-KIQCQQLVDEFFDIILNFVLSELDVDKVCKVMGMCSM-KKVSGIE---------------CEVCERVMDEVIQLAFSNHSQQVVSNFIENKICPRL--GFVKTECTSLIDTYLPVIFSLARSTLNSKVICAELKLCS-----------------LISQKKINLLMKSNINDDSAK------------------------CKICHFVVSELLTLLTNPDIQKSIENFLENHVCPHFGAK-KQECINDINQYYSLVINIITEFLKP-TLCNELRLCNSTTTSIIMSQPLVKMSRAILSSNCQLCEKFYFHLQKIFLKNTPISPLT-VFANVCNLEDTDETNECLKHFRNLNSIVAAFHASR-NIGEFCKMTEMC-KGSKKHHVG 2461
CD C ++ VEA + + ++ + A +C+ L ++ C++ +N + P + R L E PE IC +Q+C T+ +K+ + Q + C C+ V ++S+ + IC+ LP+ +++ C + Y + L+ LD +C I LC+ +E+ + + C+ C V+ ++ L E V+ VCS FF I C +V +N L L IC +C T+ KKP A G I CDLC+ + S + K ++ ++ + ++C +ISSQC LV YA Q+I I+ + + IC + C + +Q G E CD C + + + I+ L ++C +F + QC+QLV ++ I+ ++ EL +++C ++G+CS KKV G E C++C+ V+ V L N + + + +++C RL + ++C L+ Y P I L + IC+ L LCS IS + ++ N + C +C V+ ++ TLL IE L + VC F A +C + QY +I++I E L+P +C+ L+LC+ + + + P+ C LC++ L + KN + + VC L ++C + + + A E C + ++C +G KK G
Sbjct: 588 CDICRFALEVVEAIVSRNRTEQSLVEALQQVCSLLTPSARPQCEQFINNYMPTLIRLLLQELDPEVICNELQLCNETD----NKVPETAHLQAASIGSIECEVCELVLGAIDSVLDGNRTLAAIEKVLGEICSILPATIRAQCVSFVSEYGPAVMELLADELDPQVVCIKIGLCNQAQVKQLLEAEDKRRPQATPVCELCNQVMQLIETFLQQNRTEETVTKALQQVCS---FFPDTISTECRQFVGRYSQEVINLLLQDLNATDICKKLGLCSDTRPVTGKELTTTAGDKKPSA---GPI-CDLCKQVLQTLDSLLEKNRTVDDIEKAL-DRVCKLFPATISSQCRQLVGQYAPQIIEYIAEELKPEEICSLLGLCSQGNGAQETKPVATIGEEKPSAGPICDLCKEVLQTVDSLLEKNRTVDDIEKALD-QVCRLFPATISSQCRQLVAQYAPQIIEYIADELKPEEICSLLGLCSQGKKVQGKELTTATDEQKPAAGPICDLCKEVLQAVDSLLEKNRTVDDIEKAL-DQVC-RLFPATISSQCRQLVGQYAPQIIEYITEELKPEEICSLLGLCSQGNRDQGKASDGGRQTEAISWSHLRVVQSGLDNTGQSGREEQHSGQYHEAAVGELKPSAGPICDLCKEVLQKVDTLLEKNRTVDDIEKAL-DQVCRLFPASIASQCRQLVAQYAPALIDLIVEELQPDEICSLLKLCSQSNKA---AGPI-----------CDLCKQVLQTLDSLLEKNRTVDDIEKALDQVCRLFPATIASQCRQLVGQYAPQIIEYIADELKPEEICSLLKLCSQGKKKPAAG 1381 HSP 4 Score: 144.436 bits (363), Expect = 4.098e-31 Identity = 158/706 (22.38%), Postives = 293/706 (41.50%), Query Frame = 2
Query: 254 NKVDISHHKNGDY-CDACMKFVQSVEAHIPKISEQEFVNLAGNLICNNLKGKSQLDCKKEVNFFAPLIYRELSMEATPEKICGL------------------------VQVCKNTNAMSFSKIFNSIKFQNDDVCTKCKQVFQFLESMFSKVSPDEFKFLIESL--ICNQLPSEVQSTCKLLLDGYVTQLYNLIKSLLDGDRICPLIHLCSSENTQI---------TLGSWQCQTCLDVIVVLRKDLAGVSAPEIVSLIKSNVCSKLNFFEKGICNSYVSNLVPTFVNELHSTLTPQQICGNHHVCTSHSIKKPIASE-----KGVIECDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHL-KSISSQCSSLVDAYADQLITMISNHMDSKAICIAINAC--------KGFSISQRKSAPNFKGIECDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIFQSE-KIQCQQLVDEFFDIILNFVLSELDVDKVCKVMGMCSM----------------KKVSGIECEVCERVMDEVIQLAFSNHSQQVVSNFIENKICPRL--GFVKTECTSLIDTYLPVIFSLARSTLNSKVICAELKLCSL---ISQKKINLLMKSNINDDSAKCKICHFVVSELLTLLTNPDIQKSIENFLENHVCPHFGAK-KQECINDINQYYSLVINIITEFLKP-TLCNELRLCN 2149
N V K GD C+ CM V ++ Q V N C L G+ C+ +AP ++ ++ E PE +C L V+V + + + + +K D C C+ + +E++ S+ ++ L+E+L +C+ L + C+ ++ Y+ L L+ LD + IC + LC+ + ++ ++GS +C+ C V+ + L G + + +CS L + C S+VS P + L L PQ +C +C +K+ + +E + C+LC + ++F+ + V ++ ++CS +IS++C V Y+ ++I ++ +++ IC + C K + + P+ I CD C + + + I+ L R+C +F + QC+QLV ++ I+ ++ EL +++C ++G+CS K +G C++C+ V+ V L N + + + +++C RL + ++C L+ Y P I L + IC+ L LCS + K++ C +C V+ + +LL IE L + VC F A +C + QY +I ITE LKP +C+ L LC+
Sbjct: 456 NLVPTDVGKVGDIKCEECMAIVGFLQNLDRDAEVQTAVRKLFNDTCA-LLGQEAKQCQALAAQYAPQLFEIIASELDPESVCDLFLHCFKSSVEDDRGEDVVETNAVVVEVDPHADVQQSNAVSAEVKASIVD-CDICRFALEVVEAIVSRNRTEQS--LVEALQQVCSLLTPSARPQCEQFINNYMPTLIRLLLQELDPEVICNELQLCNETDNKVPETAHLQAASIGSIECEVCELVLGAIDSVLDGNRTLAAIEKVLGEICSILPATIRAQCVSFVSEYGPAVMELLADELDPQVVCIKIGLCNQAQVKQLLEAEDKRRPQATPVCELCNQVMQLIETFLQQNRTEETVTKALQ-QVCSFFPDTISTECRQFVGRYSQEVINLLLQDLNATDICKKLGLCSDTRPVTGKELTTTAGDKKPSAGPI-CDLCKQVLQTLDSLLEKNRTVDDIEKALD-RVCKLFPATISSQCRQLVGQYAPQIIEYIAEELKPEEICSLLGLCSQGNGAQETKPVATIGEEKPSAGPICDLCKEVLQTVDSLLEKNRTVDDIEKAL-DQVC-RLFPATISSQCRQLVAQYAPQIIEYIADELKPEEICSLLGLCSQGKKVQGKELTTATDEQKPAAGPICDLCKEVLQAVDSLLEKNRTVDDIEKAL-DQVCRLFPATISSQCRQLVGQYAPQIIEYITEELKPEEICSLLGLCS 1151 HSP 5 Score: 135.961 bits (341), Expect = 1.678e-28 Identity = 167/874 (19.11%), Postives = 338/874 (38.67%), Query Frame = 2
Query: 158 CLKGPTYWCATYQNAEKCDRITECFMGQGDQKNKVDISHHKNGDYCDACMKFVQSVEAHIPKISEQEFVNLAGNLICNNL-KGKSQLDCKKEVNFFAPLIYRELSMEATPEKICGLVQVCKNTNAMSFSKIFNSIKFQNDDVCTKCKQVFQFLESMFSKVSPD---EFKFLIESLICNQLPSEVQSTCKLLLDGYVTQLYNLIKSLLDGDRICPLIHLCSSENTQITL--------------------------GSWQCQTCLDVIVVLRKDLAGVSAPEIVSLIKSNVCSKLNFFEKGI----CNSYVSNLVPTFVNELHSTLTPQQICGNHHVCTSHSIKKPIASEKG---VIE---CDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHLKSISSQCSSLVDAYADQLITMISNHMDSKAICIAINACK-GFS-----ISQRKSAPNFKG----IECDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIFQSEKIQCQQLVDEFFDIILNFVLSELDVDKVCKVMGMC------------------------------------SMKKVSGIECEVCERVMDEVIQLAFSNHSQQVVSNFIENKICPRLG-FVKTECTSLIDTYLPVIFSLARSTLNSKVICAELKLCSLISQKKINLLMKSNINDDSAKCKICHFVVSELLTLLTNPDIQKSIENFLENHVCPHFGAK-KQECINDINQYYSLVINIITEFLKP-TLCNELRLCNSTTTSIIMSQPLVKMSRAILSSNCQLCEKFYFHLQKIFLKNTPISPLT-VFANVCNLEDTDETNECLKHF-RNLNSIVAAFHASRNIGEFCKMTEMCKGSKK-------HHVGKKQCSWGP 2485
C GP YWC++ A +C C G + ++ + C + VQ + +E++ +C + + + +CK+ V + PL L+ +++ ++ +C+ + +K+Q T+CK + F+ + +++ D + + +++S IC+++ +++ CKLL++ + L + I + IC L CS+ + G +C C V+ ++ G +P S+ N KL +E G+ C L L+ L P C C + P + E V E C CE + + + +++ + +F K +C ++ S+ +C+++VD L+ ISN + C ++ C G S + P G I+C+ C V ++ D +T+++ C + E QCQ L ++ + + SELD + VC + C + K S ++C++C ++ V + N ++Q + ++ ++C L + +C I+ Y+P + L L+ +VIC EL+LC+ K + S +C++C V+ + ++L +IE L +C A + +C++ +++Y V+ ++ + L P +C ++ LCN ++ + +A + C+LC + ++ +N +T VC+ + EC + R ++ N + CK +C ++ G K+ S GP
Sbjct: 24 CTWGPAYWCSSVTAAGRCSAFAHCMQGVWGKT----LADFLQTETCTTSVNKVQLARRLLVTKNEKDSATQILTSLCTLVTDSERRKECKEMVVTYLPLALELLTSSLKDDQVAWVLGLCEPAG-----RDVAPVKYQPSAEETRCKNCYTFVTDITARLGKDGGEKLQGILKSTICSEM-ADMAPVCKLLVENTIPDLLSEIANEAQPGDICRLADFCSNRGEEPKFRQLFKKTFRKLSTIISVVKAKIHDIEGRVECYECQKVVTSVKN--FGKDSPLRSSV--ENAFMKLCMYE-GLDTEECGDLSWGLFSQLDMFLYENLEPSSFCSQEFECIDSDLVPPPSVEDAQTPVYESSSCSDCEKFYSDISAILHDEDSVTRTVAFAKKTLCQNVGSLQYECNAMVDRLLPVLLGYISNSFTPASFCGSMAMCSPGLSPKFDIMKVVNLVPTDVGKVGDIKCEECMAIVGFLQNLDRDAEVQTAVRKLFN-DTCALLGQEAKQCQALAAQYAPQLFEIIASELDPESVCDLFLHCFKSSVEDDRGEDVVETNAVVVEVDPHADVQQSNAVSAEVKASIVDCDICRFALEVVEAIVSRNRTEQSLVEALQ-QVCSLLTPSARPQCEQFINNYMPTLIRLLLQELDPEVICNELQLCNETDNKVPETAHLQAASIGSIECEVCELVLGAIDSVLDGNRTLAAIEKVL-GEICSILPATIRAQCVSFVSEYGPAVMELLADELDPQVVCIKIGLCNQAQVKQLLEAEDKRRPQA--TPVCELCNQVMQLIETFLQQNRTEETVTKALQQVCSFFPDTISTECRQFVGRYSQEVINLLLQDLNATDICKKLGLCSDTRPVTGKELTTTAGDKKPSAGP 877 HSP 6 Score: 113.62 bits (283), Expect = 1.231e-21 Identity = 162/760 (21.32%), Postives = 293/760 (38.55%), Query Frame = 2
Query: 293 CDACMKFVQSVEAHI-PKISEQEFVNLAGNLICNNLKGKSQLDCKKEVNFFAPLIYRELSMEATPEKICGLVQVCKNTNAMSFS--KIFNSIKFQ----NDDVCTKCKQVFQFLESMFSKVSPDEFKFLIESLICNQLPSEVQSTCKLLLDGYVTQLYNLIKSLLDGDRICPLIHLCSSENTQITLGSWQCQTCLDVIVVLRKDLAGVSAPEIVSLIKS-----NVCSK-LNFFEKGICNSYVSNLVPTFVNELHSTLTPQQICGNHHVCTSHSIKKPIASEKGVIECDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHL-KSISSQCSSLVDAYADQLITMISNHMDSKAICIAINAC----KGFSISQRKSAPNFKGIECDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIFQSE-KIQCQQLVDEFFDIILNFVLSELDVDKVCKVMGMCSMKKVSGI-------------ECEVCERVMDEVIQLAFSNHSQQVVSNFIENKICPRLGFVKTECTSLIDTYLPVIFSLARSTLNSKVICAELKLCS---LISQKKINLLMKSNINDDSAKCKICHFVVSELLTLLTNPDIQKSIENFLENHVCPHFGAK-KQECINDINQYYSLVINIITEFLKP-TLCNELRLCNSTTTSIIMSQPLVKMSRAILSSN--CQLCEKFYFHLQKIFLKNTPISPLT-VFANVCNLEDTDETNECLKHFRNLNSIVAAFHASR-NIGEFCKMTEMCKGSKK 2449
C C KF + A + + S V A +C N+ G Q +C V+ P++ +S TP CG + +C + F K+ N + D C +C + FL+++ +EVQ+ V +L+N +LL + + QCQ LA AP++ +I S +VC L+ F+ + + ++V T N + + P + V S+++ + + +++CD+C + ++ +S+ + ++ ++CS L S QC ++ Y LI ++ +D + IC + C + A + IEC+ C L + I + +I+ L IC I + + QC V E+ ++ + ELD VC +G+C+ +V + CE+C +VM + N +++ V+ ++ + TEC + Y + +L LN+ IC +L LCS ++ K++ C +C V+ L +LL IE L + VC F A +C + QY +I I E LKP +C+ L LC S ++P+ + S+ C LC++ + + KN + + VC L +++C + + + A E C + +C KK
Sbjct: 368 CSDCEKFYSDISAILHDEDSVTRTVAFAKKTLCQNV-GSLQYECNAMVDRLLPVLLGYISNSFTPASFCGSMAMCSPGLSPKFDIMKVVNLVPTDVGKVGDIKCEECMAIVGFLQNLDRD-------------------AEVQTA--------VRKLFNDTCALLGQE-------------------AKQCQA-----------LAAQYAPQLFEIIASELDPESVCDLFLHCFKSSVEDDRGEDVVET--NAVVVEVDP-----HADVQQSNAVSAEVKA--SIVDCDICRFALEVVEAIVSRNRTEQSLVEALQ-QVCSLLTPSARPQCEQFINNYMPTLIRLLLQELDPEVICNELQLCNETDNKVPETAHLQAASIGSIECEVCELVLGAIDSVLDGNRTLAAIEKVLG-EICSILPATIRAQCVSFVSEYGPAVMELLADELDPQVVCIKIGLCNQAQVKQLLEAEDKRRPQATPVCELCNQVMQLIETFLQQNRTEETVTKALQQVCSFFPDTISTECRQFVGRYSQEVINLLLQDLNATDICKKLGLCSDTRPVTGKELTTTAGDKKPSAGPICDLCKQVLQTLDSLLEKNRTVDDIEKAL-DRVCKLFPATISSQCRQLVGQYAPQIIEYIAEELKPEEICSLLGLC-SQGNGAQETKPVATIGEEKPSAGPICDLCKEVLQTVDSLLEKNRTVDDIEKALDQVCRLFPATISSQCRQLVAQYAPQIIEYIADELKPEEICSLLGLCSQGKK 1056 HSP 7 Score: 96.2857 bits (238), Expect = 2.826e-16 Identity = 81/378 (21.43%), Postives = 159/378 (42.06%), Query Frame = 2
Query: 284 GDYCDACMKFVQSVEAHIPKISEQEFVNLAGNLICNNLKGKSQLDCKKEVNFFAPLIYRELSMEATPEKICGLVQVCKNTNAMSFSKIFNSIKFQNDDVCTKCKQVFQFLESMFSK-VSPDEFKFLIESLICNQLPSEVQSTCKLLLDGYVTQLYNLIKSLLDGDRICPLIHLCSSENTQITLGSWQCQTCLDVIVVLRKDLAGVSAPEIVSLIKSNVCSKLNFFEKGICNSYVSNLVPTFVNELHSTLTPQQICG-------NHHVCTSHSIKKPIASEKGVIECDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHLKS-ISSQCSSLVDAYADQLITMISNHMDSKAICIAINACKGFSISQRKSAPNFKGIE 1390
G CD C + +Q V+ + K + + A + +C C++ V +AP + + E P++IC L+++C +N + +C CKQV Q L+S+ K + D+ + ++ +C P+ + S C+ L+ Y Q+ I L + IC L+ LCS + G C C +V+ + L + + VC C V P + + L P++IC H+ + +A +G + C +C+ + + +++ + +V+ + + +C + ++S+CS LV + ++ +++ ++ KAIC I C S P F G++
Sbjct: 1211 GPICDLCKEVLQKVDTLLEKNRTVDDIEKALDQVCRLFPASIASQCRQLVAQYAPALIDLIVEELQPDEICSLLKLCSQSNKAAGP------------ICDLCKQVLQTLDSLLEKNRTVDDIEKALDQ-VCRLFPATIASQCRQLVGQYAPQIIEYIADELKPEEICSLLKLCSQGKKKPAAGPI-CDLCKEVLQAVDSLLEKNRTVDDIEKALDQVCRLFPATISSQCRQLVGQYAPQIIEYIAEELKPEEICSLLGLCSQGHNELGREVETQAVAVREGEL-CLICDTLLNYVEEGLNQQATVEEVEKLL-NDVCKLFPTDLTSECSLLVQQFTPLIMQLLAEEVNPKAICETIKLCPS---PNSTSHPKFLGLD 1569 HSP 8 Score: 74.7146 bits (182), Expect = 9.990e-10 Identity = 62/278 (22.30%), Postives = 117/278 (42.09%), Query Frame = 2
Query: 269 SHHKNGDYCDACMKFVQSVEAHIPKISEQEFVNLAGNLICNNLKGKSQLDCKKEVNFFAPLIYRELSMEATPEKICGLVQVCKNTNAMSFSKIFNSIKFQNDDVCTKCKQVFQFLESMFSK-VSPDEFKFLIESLICNQLPSEVQSTCKLLLDGYVTQLYNLIKSLLDGDRICPLIHLCSS---------ENTQITLGSWQ-CQTCLDVIVVLRKDLAGVSAPEIVSLIKSNVCSKLNFFEKGICNSYVSNLVPTFVNELHSTLTPQQICGNHHVCTS 1069
S+ G CD C + +Q++++ + K + + A + +C C++ V +AP I ++ E PE+IC L+++C K +C CK+V Q ++S+ K + D+ + ++ +C P+ + S C+ L+ Y Q+ I L + IC L+ LCS E + + + C C ++ + + L + E V + ++VC C+ V P + L + P+ IC +C S
Sbjct: 1290 SNKAAGPICDLCKQVLQTLDSLLEKNRTVDDIEKALDQVCRLFPATIASQCRQLVGQYAPQIIEYIADELKPEEICSLLKLCSQGKK----------KPAAGPICDLCKEVLQAVDSLLEKNRTVDDIEKALDQ-VCRLFPATISSQCRQLVGQYAPQIIEYIAEELKPEEICSLLGLCSQGHNELGREVETQAVAVREGELCLICDTLLNYVEEGLNQQATVEEVEKLLNDVCKLFPTDLTSECSLLVQQFTPLIMQLLAEEVNPKAICETIKLCPS 1556
BLAST of Proactivator polypeptide vs. TrEMBL
Match: R7VKI2 (Uncharacterized protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_225175 PE=4 SV=1) HSP 1 Score: 169.859 bits (429), Expect = 1.282e-40 Identity = 141/570 (24.74%), Postives = 243/570 (42.63%), Query Frame = 2
Query: 1019 STLTPQQICGNHHVCTSHSIKKPIASEKGVIE-CDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHLKS--ISSQCSSLVDAYADQLITMISNHMDSKAICIAINACKGFSISQRKSAPNFK----GIE--CDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIFQSEKIQCQQLVDEFFDIILNFVLSELDVDKVCKVMGMCSMKKVSGIE-------------------------CEVCERVMDEVIQLAFSNHSQQVVSNFIENKICPRLGFVKTECTSLIDTYLPVIFSLARSTLNSKVICAELKLCSLISQKK-----INLLMKSNINDDSAKCKICHFVVSELLTLLTNPDIQKSIENFLENHVCPHFGAKKQE-CINDINQYYSLVINIITEFLKPT-LCNELRLCNSTTTSIIMSQPLVKMSRAILSSNCQLCEKFYFHLQKIFLKNTPISPL-TVFANVCNLEDTDETNECLKHFRNLN-SIVAAFHASRNIGEFC------------KMTEMCKGSKKHHVGKKQCSWGPGFWCISRQHAAGCGPGAVKHCEMKVW 2563
S++ + C C S + K +K E C CE V KS + + ++V+ F+ S CS + S ++ +C+S VD Y ++++ +I+ MD + +C + C G + + K P +E C C IK YI+ + + I+ + ++C + +C+QLV F IL + D D +C G+C +SG C +CE V+ EV L +Q + F++ ++C LG VK C +D+Y + F + L+ K C L C S + + L + S C +C FV+SE+ +LL++ ++ I+ LE VC Q+ C++ +N Y LVIN++T L P +C + LC + + + + + + C +CE ++ + +N+ I+ + V VCN T +C +IV + E C +M E+ K + +G +C++GP FWC S+++A C V HCE W
Sbjct: 22 SSIANSKQCNAFDHCVSSTWKNNEIVDKDTDEVCQFCETVVGEVKSMLLNKKAQDEVRQFLDSA-CSVIPSAELAKECASTVDKYLEEILGLIAMEMDPQMVCSLMGLCTGLN-KENKPTPQLAVSPVNVEPLCTDCKKFFEDIKAYITSASTEKEIEEMIDDQLCTNLGGLEDECKQLVKTFLPEILQALAGAYDPDIICDAFGLCLNSSLSGARTLFHRLKLQKTPLFSAAKNSNSAETCMLCETVVGEVQTLTRDAATQAEIEAFLKTEVCAHLGSVKDACEITVDSYGSIFFEFLANELDPKTRCTSLGFCLAASNEVSPSHVFSPLKAPALVKASTTCVVCEFVMSEIDSLLSDNATEEDIKIALE-KVCKILPETIQDQCMDFVNMYSDLVINLLTHELNPEQICTAIGLCRTAKS--------IPVKAIVNDAMCSVCETVIQYVDTLLEENSTIAEIEAVLEKVCNFLPTSLQQQCDTIIETYGKTIVQMIVDDASPEEICTAIGLCTSVKTVEMVELIKPQNQLALGTNECTYGPSFWCASKENAVKC--NTVAHCEKHGW 578 HSP 2 Score: 131.339 bits (329), Expect = 5.808e-28 Identity = 118/492 (23.98%), Postives = 214/492 (43.50%), Query Frame = 2
Query: 569 NDDVCTKCKQVFQFLESMF-SKVSPDEFKFLIESLICNQLPS-EVQSTCKLLLDGYVTQLYNLIKSLLDGDRICPLIHLCSSEN------TQITLGSWQ----CQTCLDVIVVLRKDLAGVSAP-EIVSLIKSNVCSKLNFFEKGICNSYVSNLVPTFVNELHSTLTPQQICGNHHVCTSHSI-------------KKPIAS----EKGVIECDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHLKSISSQCSSLVDAYADQLITMISNHMDSKAICIAINACKGFSISQRKS-------APNF--KGIECDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIF-QSEKIQCQQLVDEFFDIILNFVLSELDVDKVCKVMGMCSMKK-------VSGIECEVCERVMDEVIQLAFSNHSQQVVSNFIENKICPRLGF-VKTECTSLIDTYLPVIFSLARSTLNSKVICAELKLCSLISQKKINLLMK 1900
D+VC C+ V ++SM +K + DE + ++S C+ +PS E+ C +D Y+ ++ LI +D +C L+ LC+ N Q+ + C C ++ + S EI +I +C+ L E C V +P + L P IC +C + S+ K P+ S C LCE V ++ ++++F+K+++C+HL S+ C VD+Y ++N +D K C ++ C S S AP C C +++I +SD A + IK L+ ++C I ++ + QC V+ + D+++N + EL+ +++C +G+C K V+ C VCE V+ V L N + + +E K+C L ++ +C ++I+TY I + + + IC + LC+ + ++ L+K
Sbjct: 51 TDEVCQFCETVVGEVKSMLLNKKAQDEVRQFLDS-ACSVIPSAELAKECASTVDKYLEEILGLIAMEMDPQMVCSLMGLCTGLNKENKPTPQLAVSPVNVEPLCTDCKKFFEDIKAYITSASTEKEIEEMIDDQLCTNLGGLEDE-CKQLVKTFLPEILQALAGAYDPDIICDAFGLCLNSSLSGARTLFHRLKLQKTPLFSAAKNSNSAETCMLCETVVGEVQTLTRDAATQAEIEAFLKTEVCAHLGSVKDACEITVDSYGSIFFEFLANELDPKTRCTSLGFCLAASNEVSPSHVFSPLKAPALVKASTTCVVCEFVMSEIDSLLSDNATEEDIKIALE-KVCKILPETIQDQCMDFVNMYSDLVINLLTHELNPEQICTAIGLCRTAKSIPVKAIVNDAMCSVCETVIQYVDTLLEENSTIAEIEAVLE-KVCNFLPTSLQQQCDTIIETYGKTIVQMIVDDASPEEICTAIGLCTSVKTVEMVELIK 538 HSP 3 Score: 113.235 bits (282), Expect = 3.993e-22 Identity = 115/541 (21.26%), Postives = 237/541 (43.81%), Query Frame = 2
Query: 143 AFDFD-CLKGPTYWCATYQNAEKCDRITECFMGQGDQKNKVDISHHKNGDYCDACMKFVQSVEAHIPKISE-QEFVNLAGNLICNNLKGKSQLDCKKEVNFFAPLIYRELSMEATPEKICGLVQVCKNTNAMSFSKIFNSIKFQN-DDVCTKCKQVFQFLESMFSKVSPD-EFKFLIESLICNQLPSEVQSTCKLLLDGYVTQLYNLIKSLLDGDRICPLIHLC------------------------SSENTQITLGSWQCQTCLDVIVVLRKDLAGVSAPEIVSLIKSNVCSKLNFFEKGICNSYVSNLVPTFVNELHSTLTPQQICGNHHVCTSHS-----------IKKPIASEKGVIECDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHL-KSISSQCSSLVDAYADQLITMISNHMDSKAICIAINACKGFSISQRKSAPNFKGIECDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIF-QSEKIQCQQLVDEFFDIILNFVLSELDVDKVCKVMGMC-SMKKVSGIE 1639
+ D D C GP++WC++ N+++C+ C VD + +C+ + V+S+ + E ++F++ A ++I + K +C V+ + I ++ME P+ +C L+ +C N + ++ N + +CT CK+ F+ +++ + S + E + +I+ +C L ++ CK L+ ++ ++ + D D IC LC +++N+ C+T + + L +D A + EI + +K+ VC+ L K C V + F L + L P+ C + C + S +K P A K C +CE ++ S +S +K ++ K+C L ++I QC V+ Y+D +I ++++ ++ + IC AI C+ K+ N C C + + + + + I+ L+ ++C S + QC +++ + I+ ++ + +++C +G+C S+K V +E
Sbjct: 7 SLDADHCTLGPSFWCSSIANSKQCNAFDHCVSSTWKNNEIVDKDTDEVCQFCETVVGEVKSMLLNKKAQDEVRQFLDSACSVIPSAELAK---ECASTVDKYLEEILGLIAMEMDPQMVCSLMGLCTGLNKENKPTPQLAVSPVNVEPLCTDCKKFFEDIKAYITSASTEKEIEEMIDDQLCTNLGG-LEDECKQLVKTFLPEILQALAGAYDPDIICDAFGLCLNSSLSGARTLFHRLKLQKTPLFSAAKNSNSAETCMLCETVVGEVQTLTRDAA--TQAEIEAFLKTEVCAHLGSV-KDACEITVDSYGSIFFEFLANELDPKTRCTSLGFCLAASNEVSPSHVFSPLKAP-ALVKASTTCVVCEFVMSEIDSLLSDNATEEDIKIALE-KVCKILPETIQDQCMDFVNMYSDLVINLLTHELNPEQICTAIGLCRTAKSIPVKAIVN--DAMCSVCETVIQYVDTLLEENSTIAEIEAVLE-KVCNFLPTSLQQQCDTIIETYGKTIVQMIVDDASPEEICTAIGLCTSVKTVEMVE 535 HSP 4 Score: 68.9366 bits (167), Expect = 3.684e-8 Identity = 48/201 (23.88%), Postives = 93/201 (46.27%), Query Frame = 2
Query: 278 KNGDYCDACMKFVQSVEAHIPKISEQEFVNLAGNLICNNLKGKSQLDCKKEVNFFAPLIYRELSMEATPEKICGLVQVCKNTNAMSFSKIFNSIKFQNDDVCTKCKQVFQFLESMFSKVSP-DEFKFLIESLICNQLPSEVQSTCKLLLDGYVTQLYNLIKSLLDGDRICPLIHLCSSENT-----------QITLGSWQC 844
K C C + +++ + + +E + +A +C L Q C VN ++ L+ L+ E PE+IC + +C+ ++ I ND +C+ C+ V Q+++++ + S E + ++E +CN LP+ +Q C +++ Y + +I + IC I LC+S T Q+ LG+ +C
Sbjct: 357 KASTTCVVCEFVMSEIDSLLSDNATEEDIKIALEKVCKILPETIQDQCMDFVNMYSDLVINLLTHELNPEQICTAIGLCRTAKSIPVKAIV------NDAMCSVCETVIQYVDTLLEENSTIAEIEAVLEK-VCNFLPTSLQQQCDTIIETYGKTIVQMIVDDASPEEICTAIGLCTSVKTVEMVELIKPQNQLALGTNEC 550
BLAST of Proactivator polypeptide vs. Ensembl Cavefish
Match: psap (prosaposin [Source:ZFIN;Acc:ZDB-GENE-020108-1]) HSP 1 Score: 68.1662 bits (165), Expect = 1.964e-11 Identity = 110/533 (20.64%), Postives = 198/533 (37.15%), Query Frame = 2
Query: 158 CLKGPTYWCATYQNAEKCDRITECFMGQGDQKNKVDISHHKNGDYCDACMKFVQSVEAHIPKISEQEFVNLAGNLICNNLKGKS-QLDCKKEVNFFAPLIYRELSME-ATPEKICGLVQVCKNTNAMSFSKI---FNSI---------------------------KFQNDDVCTKCKQVFQFLESMFSKVSPDEFKFLIESLICNQLPSEVQSTCKLLLDGYVTQLYNLIKSLLDGDRICPLIHLCSSENTQITLGSWQCQTCLDVIVVLRKDLAGVSAPEIVSLIKSNVCSKLNFFEKGICNSYVSNLVPTFVNELHSTLTPQQICGNHHVCTSHSIKKPIASEKGVIECDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHLKS-ISSQCSSLVDAYADQLITMISNHMDSKAICIAINACKGFS---ISQRKSAPNFKGIECDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIFQSE-KIQCQQLVDEFFDIILNFVLSELDVDKVCKVMGMCS--MKKVSGIE 1639
C GP YWC + A C + C Q+N + K+ CD C + + V+ + S + + C + ++ +CK+ V+ + P++ + E P +CG + +C + + +K+ FN I K ++DDVC C + F + +E K S N L ++++ C LL G + +L IC C + + + +A + P + SL + V + L P K + + +C +CE + + I +V ++ K+C+ L S +++QC L++ Y +I ++ D K IC + CKG S I + A G CD C +AV + + A + I+ ++ ++C E K QC QLV ++ +++ +L LD D VC +G C +K++ G+E
Sbjct: 23 CAHGPPYWCKNVKTASLCGAVLHC------QQNVWNQPQMKSVP-CDLCKEVLVVVDQLLKDNSTEAEILGYMEKTCQLIPDENLAAECKELVDNYYPILMGIIKGELEDPSVVCGALGLCVSQEE-ALAKLQLQFNEIPKVDLNQRISPFLLNVPQLLYPQETDKKEEDDDVCEDCVK--------FIAAAQEEAKQ--NSTFVNSLIQQIEAQCDLLGPGLSDMVMIHYSCVLQPKDICSRAGFCDGTPKSVPMQTL---------------VAAKTIPAVKSLPATKVVEA-------------------------TPLKP---------------AKKMVRAREPPQCAICEYVMKELEDMIQDQTTEEEVMQAVE-KVCNILPSTLTAQCKDLIETYGQAIIELLVQEADPKTICTVLGLCKGASRAFIPVMEKADFQAGGFCDVCKMAVRYVDGILEQNATEAQIEEAVR-KVCNFLPDEFKTQCDQLVQQYEPLLIQLLLQTLDPDFVCMKLGACPEVLKQLLGME 480 HSP 2 Score: 67.3958 bits (163), Expect = 3.699e-11 Identity = 62/282 (21.99%), Postives = 117/282 (41.49%), Query Frame = 2
Query: 1733 ICPRLGFVKTECTSLIDTYLPVIFSLARSTLNSKVICAELKLCSLISQKKINLLMKSNINDDSAKCKICHFVVSELLTLLTNPDIQKSIENFLENHVCPHFGAK-KQECINDINQYYSLVINIITEFLKP-TLCNELRLCNSTTTSIIMSQPLVKMSRAILSSNCQLCEKFYFHLQKIFLKNTPISPLT-VFANVCNLEDTDETNECLKHFRNLNSIVAAFHASRNIGEF-CKMTEMCKGSKKHHVGKKQCSWGPGFWCISRQHAAGCGPGAVKHCEMKVWN 2566
IC R GF S+ P+ +A T+ + K+ K ++++ + +C IC +V+ EL ++ + ++ + +E VC + +C + I Y +I ++ + P T+C L LC + + I P+++ + C +C+ ++ I +N + + VCN + +C + + ++ +F C C K +G +QCSWGP FWC + + A+ C AV HC VW+
Sbjct: 244 ICSRAGFCDGTPKSV-----PMQTLVAAKTIPAVKSLPATKVVEATPLKPAKKMVRAR---EPPQCAICEYVMKELEDMIQDQTTEEEVMQAVEK-VCNILPSTLTAQCKDLIETYGQAIIELLVQEADPKTICTVLGLCKGASRAFI---PVMEKADFQAGGFCDVCKMAVRYVDGILEQNATEAQIEEAVRKVCNFLPDEFKTQCDQLVQQYEPLLIQLLLQTLDPDFVCMKLGACPEVLKQLLGMEQCSWGPTFWCKNLETASRC--NAVAHCRRHVWS 511 HSP 3 Score: 56.6102 bits (135), Expect = 9.353e-8 Identity = 47/184 (25.54%), Postives = 78/184 (42.39%), Query Frame = 2
Query: 293 CDACMKFVQSVEAHIPKISEQEFVNLAGNLICNNLKGKSQLDCKKEVNFFAPLIYRELSMEATPEKICGLVQVCKNTNAMSFSKIFNSIKFQNDDVCTKCKQVFQFLESMFSKVSPDEFKFLIESLICNQLPSEVQSTCKLLLDGYVTQLYNLIKSLLDGDRICPLIHLCSSENTQITLGSWQC 844
C C ++ +E I + +E V A +CN L CK + + I L EA P+ IC ++ +CK + +F + FQ C CK ++++ + + + + +CN LP E ++ C L+ Y L L+ LD D +C + C Q+ LG QC
Sbjct: 301 CAICEYVMKELEDMIQDQTTEEEVMQAVEKVCNILPSTLTAQCKDLIETYGQAIIELLVQEADPKTICTVLGLCKGAS-RAFIPVMEKADFQAGGFCDVCKMAVRYVDGILEQNATEAQIEEAVRKVCNFLPDEFKTQCDQLVQQYEPLLIQLLLQTLDPDFVCMKLGACPEVLKQL-LGMEQC 482 HSP 4 Score: 53.5286 bits (127), Expect = 9.263e-7 Identity = 100/484 (20.66%), Postives = 177/484 (36.57%), Query Frame = 2
Query: 581 CTKCKQVFQFLESMFSKVSPDEFKFLIESLICNQLPSE-VQSTCKLLLDGYVTQLYNLIKSLL-DGDRICPLIHLCSSENTQITLGSWQCQTCLDVIVVLRKDLAGVSAPEIVSLIKSNVCSKLNFFEKGICNSYVSNLVPTFVNELHSTLTPQQICGNHHVCTSHSIKKPIASEKGVIECDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHLKSISSQCSSLVDAYADQLITMISNHMDSKAICIAINACKGF-------------SISQRKSAPNFKGIE------------------CDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIFQSE-KIQCQQLVDEFFDIILNFVLSELDVDKVCKVMGMCS---------MKKV---SGIECEVCERVMDEVIQLAFSNHSQQVVSNFIENKICPRL-GFVKTECTSLIDTYLPVIFSLARSTLNSKVICAELKLCSLISQKKINL 1891
C CK+V ++ + S + C +P E + + CK L+D Y L +IK L D +C + LC S+ L Q Q N K N +S F+ + L PQ+ ++K + D+CE V K + E + +F+ S I + I +QC L +D ++ S + K IC C G +I KS P K +E C C + ++++ I D + + ++ ++C I S QC+ L++ + I+ ++ E D +C V+G+C M+K +G C+VC+ + V + N ++ + + K+C L KT+C L+ Y P++ L TL+ +C +L C + ++ + +
Sbjct: 61 CDLCKEVLVVVDQLLKDNSTEAEILGYMEKTCQLIPDENLAAECKELVDNYYPILMGIIKGELEDPSVVCGALGLCVSQEE--ALAKLQLQ--------------------------------FNEIPKVDLNQRIS----PFLLNVPQLLYPQE------------------TDKKEEDDDVCEDCV---KFIAAAQEEAKQNSTFVNSLI----QQIEAQCDLLGPGLSDMVMIHYSCVLQPKDICSRAGFCDGTPKSVPMQTLVAAKTIPAVKSLPATKVVEATPLKPAKKMVRAREPPQCAICEYVMKELEDMIQDQTTEEEVMQAVE-KVCNILPSTLTAQCKDLIETYGQAIIELLVQEADPKTICTVLGLCKGASRAFIPVMEKADFQAGGFCDVCKMAVRYVDGILEQNATEAQIEEAV-RKVCNFLPDEFKTQCDQLVQQYEPLLIQLLLQTLDPDFVCMKLGACPEVLKQLLGM 479
BLAST of Proactivator polypeptide vs. Ensembl Cavefish
Match: sftpba (prosaposin-like [Source:NCBI gene;Acc:103040133]) HSP 1 Score: 66.6254 bits (161), Expect = 3.115e-11 Identity = 69/299 (23.08%), Postives = 118/299 (39.46%), Query Frame = 2
Query: 563 FQNDDVCTKCKQVFQFLESMFSKVSPDE-FKFLIESLICNQLPSEVQSTCKLLLDGYVTQLYNLIKSLLDGDRICPLIHLCSSENT----QITLGSWQCQTCLDVIVV----------LRKDLAGVSAPEIVSLIKS---NVCSKLNFFEKGICNSYVSNLVPTFVNELHSTLTPQQICGNHHVCTSHSIKKPIASEKGVIECDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHLKSISSQCSSLVDAYADQLITMISNHMDSKAICIAINACKGFSISQRKSAPNFKGIECDACH 1405
F +DD C C + + L+ + S+ E K L+E L C+ LP E+ TCK +D + I S++ +C + LC + ++ + Q L I V DL P+ +L+ S + C L +G C V V +N L + +P ++C +C + I P + E + +CD C + + + SF+ + SH ++ QC S YA QL ++ M A+ C+ + ++ P+ G C H
Sbjct: 37 FTDDDACQDCIHIIELLKDLISEGEFQEKIKGLLEKL-CDSLPGEIPKTCKEQVDKNLPLAITFISSIMIPGEVCAYLGLCDGQMRGQMKELIMNHMQKIVLLPDITVNSSIPCTLCTYVTDLLQCIIPKTQTLLISLLGDACQILPPLLRGQCTVLVKTYVKMLINFLLNFASPDKLCSVLRLCENLEI--PPSGEFFLSDCDSCLTLAVLTRLSLGTNATEPQAASFLHTVCQSHPDAL-PQCESFTQRYAGQLQGLLGKEM------AALQMCERARLCDDQAGPDAAGDPCTLGH 325 HSP 2 Score: 62.003 bits (149), Expect = 1.029e-9 Identity = 63/278 (22.66%), Postives = 108/278 (38.85%), Query Frame = 2
Query: 296 DACMKFVQSVEAHIPKISEQEFVNLAGNLI---CNNLKGKSQLDCKKEVNFFAPLIYRELSMEATPEKICGLVQVCKN----------TNAMSFSKIFNSIKFQNDDVCTKCKQVFQFLESMFSKVSPDEFKFLIESLICNQLPSEVQSTCKLLLDGYVTQLYNLIKSLLDGDRICPLIHLCSSENTQITLGSW----QCQTCLDVIVVLRKDLA-GVSAPEIVSLIKSNVCSKLNFFEKGICNSYVSNLVPTFVNELHSTLTPQQICGNHHVCTSHS 1075
DAC + +E ISE EF L+ C++L G+ CK++V+ PL +S P ++C + +C N M + I + CT C V L+ + K L ++ C LP ++ C +L+ YV L N + + D++C ++ LC EN +I C +CL + V+ R L + P+ S + + S + + C S+ L + Q+C +C +
Sbjct: 41 DACQDCIHIIELLKDLISEGEFQEKIKGLLEKLCDSLPGEIPKTCKEQVDKNLPLAITFISSIMIPGEVCAYLGLCDGQMRGQMKELIMNHMQKIVLLPDITVNSSIPCTLCTYVTDLLQCIIPKTQTLLISLLGDA--CQILPPLLRGQCTVLVKTYVKMLINFLLNFASPDKLCSVLRLC--ENLEIPPSGEFFLSDCDSCLTLAVLTRLSLGTNATEPQAASFLHTVCQSHPDALPQ--CESFTQRYAGQLQGLLGKEMAALQMCERARLCDDQA 312
BLAST of Proactivator polypeptide vs. Ensembl Cavefish
Match: sftpba (prosaposin-like [Source:NCBI gene;Acc:103040133]) HSP 1 Score: 65.4698 bits (158), Expect = 8.351e-11 Identity = 69/303 (22.77%), Postives = 118/303 (38.94%), Query Frame = 2
Query: 563 FQNDDVCTKCKQVFQFL-----ESMFSKVSPDEFKFLIESLICNQLPSEVQSTCKLLLDGYVTQLYNLIKSLLDGDRICPLIHLCSSENT----QITLGSWQCQTCLDVIVV----------LRKDLAGVSAPEIVSLIKS---NVCSKLNFFEKGICNSYVSNLVPTFVNELHSTLTPQQICGNHHVCTSHSIKKPIASEKGVIECDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHLKSISSQCSSLVDAYADQLITMISNHMDSKAICIAINACKGFSISQRKSAPNFKGIECDACH 1405
F +DD C C + + L E F + ++ K L+E L C+ LP E+ TCK +D + I S++ +C + LC + ++ + Q L I V DL P+ +L+ S + C L +G C V V +N L + +P ++C +C + I P + E + +CD C + + + SF+ + SH ++ QC S YA QL ++ M A+ C+ + ++ P+ G C H
Sbjct: 37 FTDDDACQDCIHIIELLKDLISEGEFQEDVQEKIKGLLEKL-CDSLPGEIPKTCKEQVDKNLPLAITFISSIMIPGEVCAYLGLCDGQMRGQMKELIMNHMQKIVLLPDITVNSSIPCTLCTYVTDLLQCIIPKTQTLLISLLGDACQILPPLLRGQCTVLVKTYVKMLINFLLNFASPDKLCSVLRLCENLEI--PPSGEFFLSDCDSCLTLAVLTRLSLGTNATEPQAASFLHTVCQSHPDAL-PQCESFTQRYAGQLQGLLGKEM------AALQMCERARLCDDQAGPDAAGDPCTLGH 329 HSP 2 Score: 60.077 bits (144), Expect = 4.996e-9 Identity = 64/282 (22.70%), Postives = 110/282 (39.01%), Query Frame = 2
Query: 296 DACMKFVQSVEAHIPKISEQEFVN-----LAGNL--ICNNLKGKSQLDCKKEVNFFAPLIYRELSMEATPEKICGLVQVCKN----------TNAMSFSKIFNSIKFQNDDVCTKCKQVFQFLESMFSKVSPDEFKFLIESLICNQLPSEVQSTCKLLLDGYVTQLYNLIKSLLDGDRICPLIHLCSSENTQITLGSW----QCQTCLDVIVVLRKDLA-GVSAPEIVSLIKSNVCSKLNFFEKGICNSYVSNLVPTFVNELHSTLTPQQICGNHHVCTSHS 1075
DAC + +E ISE EF + G L +C++L G+ CK++V+ PL +S P ++C + +C N M + I + CT C V L+ + K L ++ C LP ++ C +L+ YV L N + + D++C ++ LC EN +I C +CL + V+ R L + P+ S + + S + + C S+ L + Q+C +C +
Sbjct: 41 DACQDCIHIIELLKDLISEGEFQEDVQEKIKGLLEKLCDSLPGEIPKTCKEQVDKNLPLAITFISSIMIPGEVCAYLGLCDGQMRGQMKELIMNHMQKIVLLPDITVNSSIPCTLCTYVTDLLQCIIPKTQTLLISLLGDA--CQILPPLLRGQCTVLVKTYVKMLINFLLNFASPDKLCSVLRLC--ENLEIPPSGEFFLSDCDSCLTLAVLTRLSLGTNATEPQAASFLHTVCQSHPDALPQ--CESFTQRYAGQLQGLLGKEMAALQMCERARLCDDQA 316
BLAST of Proactivator polypeptide vs. Ensembl Cavefish
Match: sftpba (prosaposin-like [Source:NCBI gene;Acc:103040133]) HSP 1 Score: 65.4698 bits (158), Expect = 8.351e-11 Identity = 69/303 (22.77%), Postives = 118/303 (38.94%), Query Frame = 2
Query: 563 FQNDDVCTKCKQVFQFL-----ESMFSKVSPDEFKFLIESLICNQLPSEVQSTCKLLLDGYVTQLYNLIKSLLDGDRICPLIHLCSSENT----QITLGSWQCQTCLDVIVV----------LRKDLAGVSAPEIVSLIKS---NVCSKLNFFEKGICNSYVSNLVPTFVNELHSTLTPQQICGNHHVCTSHSIKKPIASEKGVIECDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHLKSISSQCSSLVDAYADQLITMISNHMDSKAICIAINACKGFSISQRKSAPNFKGIECDACH 1405
F +DD C C + + L E F + ++ K L+E L C+ LP E+ TCK +D + I S++ +C + LC + ++ + Q L I V DL P+ +L+ S + C L +G C V V +N L + +P ++C +C + I P + E + +CD C + + + SF+ + SH ++ QC S YA QL ++ M A+ C+ + ++ P+ G C H
Sbjct: 37 FTDDDACQDCIHIIELLKDLISEGEFQEDVQEKIKGLLEKL-CDSLPGEIPKTCKEQVDKNLPLAITFISSIMIPGEVCAYLGLCDGQMRGQMKELIMNHMQKIVLLPDITVNSSIPCTLCTYVTDLLQCIIPKTQTLLISLLGDACQILPPLLRGQCTVLVKTYVKMLINFLLNFASPDKLCSVLRLCENLEI--PPSGEFFLSDCDSCLTLAVLTRLSLGTNATEPQAASFLHTVCQSHPDAL-PQCESFTQRYAGQLQGLLGKEM------AALQMCERARLCDDQAGPDAAGDPCTLGH 329 HSP 2 Score: 60.077 bits (144), Expect = 4.996e-9 Identity = 64/282 (22.70%), Postives = 110/282 (39.01%), Query Frame = 2
Query: 296 DACMKFVQSVEAHIPKISEQEFVN-----LAGNL--ICNNLKGKSQLDCKKEVNFFAPLIYRELSMEATPEKICGLVQVCKN----------TNAMSFSKIFNSIKFQNDDVCTKCKQVFQFLESMFSKVSPDEFKFLIESLICNQLPSEVQSTCKLLLDGYVTQLYNLIKSLLDGDRICPLIHLCSSENTQITLGSW----QCQTCLDVIVVLRKDLA-GVSAPEIVSLIKSNVCSKLNFFEKGICNSYVSNLVPTFVNELHSTLTPQQICGNHHVCTSHS 1075
DAC + +E ISE EF + G L +C++L G+ CK++V+ PL +S P ++C + +C N M + I + CT C V L+ + K L ++ C LP ++ C +L+ YV L N + + D++C ++ LC EN +I C +CL + V+ R L + P+ S + + S + + C S+ L + Q+C +C +
Sbjct: 41 DACQDCIHIIELLKDLISEGEFQEDVQEKIKGLLEKLCDSLPGEIPKTCKEQVDKNLPLAITFISSIMIPGEVCAYLGLCDGQMRGQMKELIMNHMQKIVLLPDITVNSSIPCTLCTYVTDLLQCIIPKTQTLLISLLGDA--CQILPPLLRGQCTVLVKTYVKMLINFLLNFASPDKLCSVLRLC--ENLEIPPSGEFFLSDCDSCLTLAVLTRLSLGTNATEPQAASFLHTVCQSHPDALPQ--CESFTQRYAGQLQGLLGKEMAALQMCERARLCDDQA 316
BLAST of Proactivator polypeptide vs. Ensembl Cavefish
Match: psap (prosaposin [Source:ZFIN;Acc:ZDB-GENE-020108-1]) HSP 1 Score: 53.1434 bits (126), Expect = 9.774e-7 Identity = 92/483 (19.05%), Postives = 169/483 (34.99%), Query Frame = 2
Query: 158 CLKGPTYWCATYQNAEKCDRITECFMGQGDQKNKVDISHHKNGDYCDACMKFVQSVEAHIPKISEQEFVNLAGNLICNNLKGKS-QLDCKKEVNFFAPLIYRELSME-ATPEKICGLVQVCKNTNAMSFSKI---FNSI---------------------------KFQNDDVCTKCKQVFQFLESMFSKVSPDEFKFLIESLICNQLPSEVQSTCKLLLDGYVTQLYNLIKSLLDGDRICPLIHLCSSENTQITLGSWQCQTCLDVIVVLRKDLAGVSAPEIVSLIKSNVCSKLNFFEKGICNSYVSNLVPTFVNELHSTLTPQQICGNHHVCTSHSIKKPIASEKGVIECDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHLKS-ISSQCSSLVDAYADQLITMISNHMDSKAICIAINACKGFSISQRKSAPNFKGIECDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIFQSE 1507
C GP YWC + A C + C Q+N + K+ CD C + + V+ + S + + C + ++ +CK+ V+ + P++ + E P +CG + +C + + +K+ FN I K ++DDVC C + F + +E K S N L ++++ C LL G + +L IC C + + + +A + P + SL + V + L P K + + +C +CE + + I +V ++ K+C+ L S +++QC L++ Y +I ++ D K IC + CKG S K+ G CD C +AV + + A + I+ ++ ++C E
Sbjct: 23 CAHGPPYWCKNVKTASLCGAVLHC------QQNVWNQPQMKSVP-CDLCKEVLVVVDQLLKDNSTEAEILGYMEKTCQLIPDENLAAECKELVDNYYPILMGIIKGELEDPSVVCGALGLCVSQEE-ALAKLQLQFNEIPKVDLNQRISPFLLNVPQLLYPQETDKKEEDDDVCEDCVK--------FIAAAQEEAKQ--NSTFVNSLIQQIEAQCDLLGPGLSDMVMIHYSCVLQPKDICSRAGFCDGTPKSVPMQTL---------------VAAKTIPAVKSLPATKVVE-------------------------ATPLKP---------------AKKMVRAREPPQCAICEYVMKELEDMIQDQTTEEEVMQAVE-KVCNILPSTLTAQCKDLIETYGQAIIELLVQEADPKTICTVLGLCKGASPVMEKADFQAGGF-CDVCKMAVRYVDGILEQNATEAQIEEAVR-KVCNFLPDE 429
BLAST of Proactivator polypeptide vs. Ensembl Nematostella
Match: EDO31455 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SXX5]) HSP 1 Score: 119.013 bits (297), Expect = 3.177e-28 Identity = 109/407 (26.78%), Postives = 183/407 (44.96%), Query Frame = 2
Query: 1085 PIASEKGVIECDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHLKSISSQCSSLVDAYADQLITMISNHM-DSKAICIAINAC-------KGFSISQRKSAPNFKGI------ECDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIFQSEKIQCQQLVDEFFDIILNFVLSEL-DVDKVCKVMGMCSMKKVSGI-----------------------------ECEVCERVMDEVIQLAFSNHSQQVVSNFIENKICPRL-GFVKTECTSLIDTYLPVIFSLARSTLNSKVICAELKLCS-----LISQKKINLLMKSNINDDSAKCKICHFVVSELLTLLTNPDIQKSIENFLENHVCPHFGAK-KQECINDINQYYSLVINIITEFLKPTLCNELRLCNS 2152
P+ S K EC C+ V + S P N++ S IK C+ L +S+C SLV+ Y +I +I + + + +C I C K + P K + EC C AV + +++S+PA + I L ++C F +EK C+ +V+ F I+ ++S L D K+C +G+C+ KV + EC +CE VM E+ + N +QQ + +E K+C L +K++C + Y P + + ++ K++C L LC+ + ++ L++ SN C+IC V++ L L N + I NFLE VC + ++ EC +++Y S V+ II TLC E+ LC++
Sbjct: 17 PVVSPKD--ECTTCKELVKTIYTMASDPTAQNQILSLIKDA-CTFLGPEASECKSLVETYGKAIIQLILSQLSNPDQVCKEIGLCTSKTEMLKKIVMRHLHKTPITKPVLRSPKDECITCQEAVNTVYKFVSNPAIEDEIVALLS-KVCSEFGAEKATCEAVVNTFGKQIVQTLVSLLSDGKKLCTEIGLCTANKVKAMLVQAAASHKVMFTPKKHVLKATYPVKTSTECVLCEFVMKELDDMLSKNSTQQEIIQAVE-KVCSILPSTIKSKCDQFVQEYGPALIEILEQEVSPKLVCTTLGLCANREHRIAMRRSHKLVIGSN-----ETCEICTTVMTYLKAFLNNNATDEEIVNFLEK-VCNYLPSQIASECNAIVSEYGSTVLQIIANTDPTTLCKEIGLCSA 412 HSP 2 Score: 85.1149 bits (209), Expect = 3.051e-17 Identity = 101/395 (25.57%), Postives = 174/395 (44.05%), Query Frame = 2
Query: 575 DVCTKCKQVFQFLESMFSKVSPDEFKFLIESLICNQLPSEVQSTCKLLLDGYVTQLYNLIKSLL-DGDRICPLIHLCSSEN----------------TQITLGSW--QCQTCLDVIVVLRKDLAGVS-APEIVSLIKSNVCSKLNFFEKGICNSYVSNLVPTFVNELHSTLTP-QQICGNHHVCTSHSIK---------------------KPIASEKGVIECDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHLKS-ISSQCSSLVDAYADQLITMISNHMDSKAICIAINAC--KGFSISQRKSAPNFKGIE--CDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIFQSE-KIQCQQLVDEFFDIILNFVLSELDVDKVCKVMGMCS 1615
D CT CK++ + + +M S + + C L E S CK L++ Y + LI S L + D++C I LC+S+ T+ L S +C TC + + + K ++ + EIV+L+ S VCS+ EK C + V+ V L S L+ +++C +CT++ +K K K EC LCE + +SK ++ ++ K+CS L S I S+C V Y LI ++ + K +C + C + I+ R+S G C+ C +T +K ++++ A I NFL+ ++C S+ +C +V E+ +L +++ D +CK +G+CS
Sbjct: 23 DECTTCKELVKTIYTMASDPTAQNQILSLIKDACTFLGPEA-SECKSLVETYGKAIIQLILSQLSNPDQVCKEIGLCTSKTEMLKKIVMRHLHKTPITKPVLRSPKDECITCQEAVNTVYKFVSNPAIEDEIVALL-SKVCSEFGA-EKATCEAVVNTFGKQIVQTLVSLLSDGKKLCTEIGLCTANKVKAMLVQAAASHKVMFTPKKHVLKATYPVKTSTECVLCEFVMKELDDMLSKNSTQQEIIQAVE-KVCSILPSTIKSKCDQFVQEYGPALIEILEQEVSPKLVCTTLGLCANREHRIAMRRSHKLVIGSNETCEICTTVMTYLKAFLNNNATDEEIVNFLE-KVCNYLPSQIASECNAIVSEYGSTVLQ-IIANTDPTTLCKEIGLCS 411 HSP 3 Score: 83.5741 bits (205), Expect = 1.009e-16 Identity = 92/396 (23.23%), Postives = 172/396 (43.43%), Query Frame = 2
Query: 839 QCQTCLDVIVVLRKDLAGVSAP-EIVSLIKSNVCSKLNFFEKGICNSYVSNLVPTFVNELHSTLT-PQQICGNHHVCTS-----------HSIKKPIASEKGVI-----ECDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHLKSISSQCSSLVDAYADQLI-TMISNHMDSKAICIAINACKGFSISQ--------------------RKSAPNFKGIECDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIFQSE-KIQCQQLVDEFFDIILNFVLSELDVDKVCKVMGMC-------SMKKV------SGIECEVCERVMDEVIQLAFSNHSQQVVSNFIENKICPRL-GFVKTECTSLIDTYLPVIFSLARSTLNSKVICAELKLCS 1864
+C TC +++ + + +A +I+SLIK + C+ L E C S V + + S L+ P Q+C +CTS H K PI K V+ EC C+ V F+S P +++ + + SK+CS + + C ++V+ + Q++ T++S D K +C I C + + + P EC C + ++ + +S + + I ++ ++C I S K +C Q V E+ ++ + E+ VC +G+C +M++ S CE+C VM + +N + + + NF+E K+C L + +EC +++ Y + + +T + +C E+ LCS
Sbjct: 24 ECTTCKELVKTIYTMASDPTAQNQILSLIK-DACTFLGP-EASECKSLVETYGKAIIQLILSQLSNPDQVCKEIGLCTSKTEMLKKIVMRHLHKTPIT--KPVLRSPKDECITCQEAVNTVYKFVSNPAIEDEIVALL-SKVCSEFGAEKATCEAVVNTFGKQIVQTLVSLLSDGKKLCTEIGLCTANKVKAMLVQAAASHKVMFTPKKHVLKATYPVKTSTECVLCEFVMKELDDMLSKNSTQQEIIQAVE-KVCSILPSTIKSKCDQFVQEYGPALIEILEQEVSPKLVCTTLGLCANREHRIAMRRSHKLVIGSNETCEICTTVMTYLKAFLNNNATDEEIVNFLE-KVCNYLPSQIASECNAIVSEYGSTVLQIIANT-DPTTLCKEIGLCS 411 HSP 4 Score: 65.4698 bits (158), Expect = 4.916e-11 Identity = 60/239 (25.10%), Postives = 105/239 (43.93%), Query Frame = 2
Query: 1637 ECEVCERVMDEVIQLAFSNHSQQVVSNFIENKICPRLGFVKTECTSLIDTYLPVIFSLARSTL-NSKVICAELKLCSLISQ--KKINL-------LMKSNINDDSAKCKICHFVVSELLTLLTNPDIQKSIENFLENHVCPHFGAKKQECINDINQYYSLVINIITEFLK--PTLCNELRLCNSTTTSIIMSQP-------------LVKMSRAI-LSSNCQLCEKFYFHLQKIFLKNT 2275
EC C+ ++ + +A +Q + + I++ C LG +EC SL++TY I L S L N +C E+ LC+ ++ KKI + + K + +C C V+ + ++NP I+ I L + VC FGA+K C +N + ++ + L LC E+ LC + ++ Q ++K + + S+ C LCE L + KN+
Sbjct: 24 ECTTCKELVKTIYTMASDPTAQNQILSLIKDA-CTFLGPEASECKSLVETYGKAIIQLILSQLSNPDQVCKEIGLCTSKTEMLKKIVMRHLHKTPITKPVLRSPKDECITCQEAVNTVYKFVSNPAIEDEIVALL-SKVCSEFGAEKATCEAVVNTFGKQIVQTLVSLLSDGKKLCTEIGLCTANKVKAMLVQAAASHKVMFTPKKHVLKATYPVKTSTECVLCEFVMKELDDMLSKNS 260 HSP 5 Score: 60.077 bits (144), Expect = 2.801e-9 Identity = 54/234 (23.08%), Postives = 102/234 (43.59%), Query Frame = 2
Query: 278 KNGDYCDACMKFVQSVEAHIPKISEQEFVNLAGNLICNNLKGKSQLDCKKEVNFFAPLIYRELSMEATPEKICGLVQVCKNTNAMSFSKIFNSIKFQNDDVCTKCKQVFQFLESMFSKVSPDEFKFLIESLICNQLPSEVQSTCKLLLDGYVTQLYNLIKSLLDGDRICPLIHLCSSENTQITLGSWQCQTCLDV-IVVLRKDLAGVSAPEIVSLIKSNV-CSKLNFFEKGICN 973
K C C ++ ++ + K S Q+ + A +C+ L + C + V + P + L E +P+ +C + +C N + + + +++ C C V +L++ + + DE +CN LPS++ S C ++ Y + + +I + D +C I LCS+ N + + L IV R DL + AP + ++ V C ++F EK N
Sbjct: 237 KTSTECVLCEFVMKELDDMLSKNSTQQEIIQAVEKVCSILPSTIKSKCDQFVQEYGPALIEILEQEVSPKLVCTTLGLCANREHRIAMRRSHKLVIGSNETCEICTTVMTYLKAFLNNNATDEEIVNFLEKVCNYLPSQIASECNAIVSEYGSTVLQIIAN-TDPTTLCKEIGLCSA-NQHVMKAKILSKHILKAKIVAKRDDLCYLGAPYWCADRRNAVNCEAVHFCEKHAWN 468
BLAST of Proactivator polypeptide vs. Ensembl Nematostella
Match: EDO39178 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SAT7]) HSP 1 Score: 72.0182 bits (175), Expect = 2.739e-13 Identity = 79/321 (24.61%), Postives = 131/321 (40.81%), Query Frame = 2
Query: 1391 CDACHLAVTKIKEYISDPARKTSIKNFLKMRICP----IFQSEKIQCQQLVDEFF-------DIILN-----------------FVLSEL-----------DVDKVCKVMGMC------------------SMKKVSGIE-CEVCERVMDEVIQLAFSNHSQQVVSNFIENKICPRLGFVKTECTSLIDTYLPVIFS-LARSTLNSKVICAELKLCSLISQKKINLLMKSNINDDSAKCKICHFVVSELLTLLTNPDIQKSIENFLENHVCPHFGAK-KQECINDINQYYSLVINIITEFLKPT-LCNELRLCNSTTTSII 2170
C C V K+K+ + D + + +K L IC F E C+++VD +F DIIL VL L D +VC +G+C S+ V E C +CE +D++ + N Q + +E+ G C +L+D Y P+I S L + NSK +C L LCS + + + DD+ +C +C FV+ E+ LL Q+ IE L VC + C + +Y ++++++ E + P +C+ + LCNS +++
Sbjct: 60 CAICEAVVGKVKDALDDKSMEGKVKAILD-EICDKDGGFFAGE---CKKVVDTYFTMIISQLDIILQNPKQVCTTLGLCSAEAEEVLKRLLWQALEGGDQQDSKQVCTELGLCIAGNRKAMMDNLIQHVLRSLPSVHNSEVCSICELAVDKIRDVIGDNSIQAEIKGVLEDACVKEGGAYAGVCKALVDQYFPIIISHLDKLVQNSKQVCTALGLCS--ADRWV-----CPRPDDAPQCVLCEFVMKEIKQLLAKDTTQQGIEKAL-MMVCSIMPETIRNNCDKFVTEYTPIIMSLLLEEVDPAKVCSMIGLCNSPAAALV 368 HSP 2 Score: 69.707 bits (169), Expect = 1.684e-12 Identity = 78/364 (21.43%), Postives = 147/364 (40.38%), Query Frame = 2
Query: 551 NSIKFQNDDVCTKCKQVFQFLESMFSKVSPDEFKFLIESLICNQLPSEVQSTCKLLLDGYVTQLYNLIKSLLDGDR-ICPLIHLCSSENTQITLGSWQCQTCLDVIVVLRKDLAGVSAPEIVSLIKSNVCSKLNFFEKGICNSYVSNLVPTFVNELHSTLTPQQICGNHHVCTSHSIKKPIASEKGVIECDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHLKSISSQCSSLVDAYADQLITMISNHMD-----SKAICIAINACKG--FSISQRKSAPNFKGIECDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIF-QSEKIQCQQLVDEFFDIILNFVLSELDVDKVCKVMGMCS 1615
NS+ N D C C+ V ++ S + I IC++ CK ++D Y T + + + +L + +C + LCS+E ++ + +L + L G + VC++L G + + NL+ + L S N VC+ +CE+ V + I ++K ++ + + C +LVD Y +I +H+D SK +C A+ C + + AP +C C + +IK+ ++ + I+ L M +C I ++ + C + V E+ II++ +L E+D KVC ++G+C+
Sbjct: 50 NSVWKYNQDTCAICEAVVGKVKDALDDKSMEGKVKAILDEICDKDGGFFAGECKKVVDTYFTMIISQLDIILQNPKQVCTTLGLCSAEAEEV------------LKRLLWQALEGGDQQD-----SKQVCTELGLCIAGNRKAMMDNLIQHVLRSLPSV-------HNSEVCS------------------ICELAVDKIRDVIGDNSIQAEIKGVLEDACVKEGGAYAGVCKALVDQY----FPIIISHLDKLVQNSKQVCTALGLCSADRWVCPRPDDAP-----QCVLCEFVMKEIKQLLAKDTTQQGIEKAL-MMVCSIMPETIRNNCDKFVTEYTPIIMSLLLEEVDPAKVCSMIGLCN 361 HSP 3 Score: 65.4698 bits (158), Expect = 4.013e-11 Identity = 80/396 (20.20%), Postives = 154/396 (38.89%), Query Frame = 2
Query: 158 CLKGPTYWCATYQNAEKCDRITECFMGQGDQKNKVDISHHKNGDYCDACMKFVQSVEAHIPKISEQEFVNLAGNLICNNLKGKSQLDCKKEVNFFAPLIYRELS-MEATPEKICGLVQVCKNTNAMSFSKIFNSIKFQNDDVCTKCKQVFQFLESMFSKVSPDEFKFLIESLICNQLPSEVQSTCKLLLDGYVTQLYNLIKSLLDGDRICPLIHLCSSENTQITLGSWQCQTCLDVIVVLRKDLAGVSAPEIVSLIKSNVCSKLNFFEKGICNSYVSNLVPTFVNELHSTL-TPQQICGNHHVCTSHSIKKPIASEKGVIECDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHL-KSISSQCSSLVDAYADQLITMISNHMDSKAICIAINAC 1336
C+ GP YWC + ++A++CD + C KN V N D C C V V+ + S + V + IC+ G +CKK V+ + +I +L + P+++C + +C ++ +Q LE + S +C +L + K ++D NLI+ +L R P +H N+++ C C + +R + S + + + C K G+C + V P ++ L + +Q+C +C++ P + +C LCE + K ++K ++ + +CS + ++I + C V Y +++++ +D +C I C
Sbjct: 25 CVYGPAYWCRSLEHAQECDAVEHC-------KNSV---WKYNQDTCAICEAVVGKVKDALDDKSMEGKVKAILDEICDKDGGFFAGECKKVVDTYFTMIISQLDIILQNPKQVCTTLGLCSAEAEEVLKRLL-----------------WQALEGGDQQDSKQ---------VCTELGLCIAGNRKAMMD-------NLIQHVL---RSLPSVH-----NSEV------CSICELAVDKIRDVIGDNSIQAEIKGVLEDACVKEGGAYAGVCKALVDQYFPIIISHLDKLVQNSKQVCTALGLCSADRWVCPRPDDAP--QCVLCEFVMKEIKQLLAKDTTQQGIEKAL-MMVCSIMPETIRNNCDKFVTEYTPIIMSLLLEEVDPAKVCSMIGLC 360
BLAST of Proactivator polypeptide vs. Ensembl Nematostella
Match: EDO38309 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SDD7]) HSP 1 Score: 65.0846 bits (157), Expect = 4.631e-11 Identity = 48/177 (27.12%), Postives = 88/177 (49.72%), Query Frame = 2
Query: 1640 CEVCERVMDEVIQLAFSNHSQQVVSNFIENKICPRL-GFVKTECTSLIDTYLPVIFSLARSTLNSKVICAELKLCSLISQKKINL--LMKSNINDDSAKCKICHFVVSELLTLLTNPDIQKSIENFLENHVC---PHFGAKKQECINDINQYYSLVINIITEFLKPTLCNELRLCNS 2152
C +CE VM E+ ++ N +++ + + NK+C + G +++EC + + Y P I + +++C ELKLCS + + L+K + + C +C VV ++ + L + K IE+ L + +C P KKQ C I Y +I+ + T+C +L +C+S
Sbjct: 199 CVMCEFVMRELSKMLNENSTKEEIETAL-NKLCSYMPGSIQSECKTFVQEYTPFIIEILSKEFKPELVCRELKLCSAGGATYLAVAKLLKPRLTANKG-CDVCQTVVKDVQSALKDDVTVKGIEDML-SMICDYIPEVEIKKQ-CSVAIQHYVPELIDFFIKQDPKTICKDLDMCSS 371 HSP 2 Score: 58.9214 bits (141), Expect = 4.628e-9 Identity = 65/309 (21.04%), Postives = 133/309 (43.04%), Query Frame = 2
Query: 1613 SMKKVSGIECEVCERVMDEVIQLAFSNHSQQVVSNFIENKICPRL-GFVKTECTSLIDTYLPVIF-SLARSTLNSKVICAELKLCSLISQKKI----------------NLLMKSNIN-------------DDSAKCKICHFVVSELLTLLTNPDIQKSIENFLENHVCPHF-GAKKQECINDINQYYSLVINIITEFLKPTL-CNELRLCNSTTTSIIMSQPLVKMSRAILSSNCQLCEKFYFHLQKIFLKNTPISPLT-VFANVCN-LEDTDETNECLKHFRNLNSIVAAFHASRNIGEFCKMTEMC 2434
S+K+ + C+ C+ V+ ++ + A Q + + +K C L + +C ++ + +F SL + +C +LKLCS S+++I +LLM + S C +C FV+ EL +L ++ IE L N +C + G+ + EC + +Y +I I+++ KP L C EL+LC++ + + L+K R + C +C+ +Q + + + + + +C+ + + + +C ++ + F ++ CK +MC
Sbjct: 64 SIKEKNSFPCDTCKEVIGKIKKFAEDESLQDKIIQTM-DKACSLLPSELSAKCKEVMGEAIKKLFASLDSIVKDPAALCKKLKLCSAQSKEEILKAVIKHLLSKDQQVSSLLMPGPVMMVPSRKPVHKLMIKTSETCVMCEFVMRELSKMLNENSTKEEIETAL-NKLCSYMPGSIQSECKTFVQEYTPFIIEILSKEFKPELVCRELKLCSAGGATYLAVAKLLK-PRLTANKGCDVCQTVVKDVQSALKDDVTVKGIEDMLSMICDYIPEVEIKKQCSVAIQHYVPELIDFFIKQDPKTICKDLDMC 369 HSP 3 Score: 54.299 bits (129), Expect = 1.221e-7 Identity = 71/349 (20.34%), Postives = 135/349 (38.68%), Query Frame = 2
Query: 158 CLKGPTYWCATYQNAEKCDRITECFMGQGDQKNKVDISHHKNGDYCDACMKFVQSVEAHIPKISEQEFVNLAGNLICNNLKGKSQLDCKKEVNFFAPLIYREL-SMEATPEKICGLVQVC------------------KNTNAMSF-----------SKIFNSIKFQNDDVCTKCKQVFQFLESMFSKVSPDEFKFLIESLICNQLPSEVQSTCKLLLDGYVTQLYNLIKSLLDGDRICPLIHLCSSENT-----------QITL--GSWQCQTCL-DVIVVLRKDLAGVSAPEIVSLIKSNVCSKLNFFE-KGICNSYVSNLVPTFVNELHSTLTPQQICGNHHVCTS 1069
C GP+YWC A +CD + C + KN + KN CD C + + ++ S Q+ + + C+ L + CK+ + ++ L S+ P +C +++C K+ S K + + + + C C+ V + L M ++ S E + +C+ +P +QS CK + Y + ++ + +C + LCS+ ++T G CQT + DV L+ D+ +++S+I C + E K C+ + + VP ++ P+ IC + +C+S
Sbjct: 33 CTWGPSYWCQGMAQAVECDAVKHC--QEKVWKNSI---KEKNSFPCDTCKEVIGKIKKFAEDESLQDKIIQTMDKACSLLPSELSAKCKEVMGEAIKKLFASLDSIVKDPAALCKKLKLCSAQSKEEILKAVIKHLLSKDQQVSSLLMPGPVMMVPSRKPVHKLMIKTSETCVMCEFVMRELSKMLNENSTKEEIETALNKLCSYMPGSIQSECKTFVQEYTPFIIEILSKEFKPELVCRELKLCSAGGATYLAVAKLLKPRLTANKGCDVCQTVVKDVQSALKDDVTVKGIEDMLSMI----CDYIPEVEIKKQCSVAIQHYVPELID-FFIKQDPKTICKDLDMCSS 371 HSP 4 Score: 53.1434 bits (126), Expect = 3.319e-7 Identity = 46/183 (25.14%), Postives = 89/183 (48.63%), Query Frame = 2
Query: 278 KNGDYCDACMKFVQSVEAHIPKISEQEFVNLAGNLICNNLKGKSQLDCKKEVNFFAPLIYRELSMEATPEKICGLVQVCKNTNA--MSFSKIFNSIKFQNDDVCTKCKQVFQFLESMF-SKVSPDEFKFLIESLICNQLPS-EVQSTCKLLLDGYVTQLYN-LIKSLLDGDRICPLIHLCSSE 811
K + C C ++ + + + S +E + A N +C+ + G Q +CK V + P I LS E PE +C +++C A ++ +K+ + + C C+ V + ++S V+ + ++ S+IC+ +P E++ C + + YV +L + IK D IC + +CSS+
Sbjct: 194 KTSETCVMCEFVMRELSKMLNENSTKEEIETALNKLCSYMPGSIQSECKTFVQEYTPFIIEILSKEFKPELVCRELKLCSAGGATYLAVAKLLKP-RLTANKGCDVCQTVVKDVQSALKDDVTVKGIEDML-SMICDYIPEVEIKKQCSVAIQHYVPELIDFFIKQ--DPKTICKDLDMCSSK 372 HSP 5 Score: 48.9062 bits (115), Expect = 6.161e-6 Identity = 19/40 (47.50%), Postives = 24/40 (60.00%), Query Frame = 2
Query: 2447 KHHVGKKQCSWGPGFWCISRQHAAGCGPGAVKHCEMKVWN 2566
K +G K+C+WGP +WC A C AVKHC+ KVW
Sbjct: 25 KFLLGSKKCTWGPSYWCQGMAQAVEC--DAVKHCQEKVWK 62
BLAST of Proactivator polypeptide vs. Ensembl Medaka
Match: psap (prosaposin [Source:NCBI gene;Acc:101163196]) HSP 1 Score: 101.293 bits (251), Expect = 9.010e-22 Identity = 102/487 (20.94%), Postives = 192/487 (39.43%), Query Frame = 2
Query: 1370 PNFKGIECDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIFQSEKI--QCQQLVDEFFDIILNFVLSEL-DVDKVCKVMGMCSMKKVSGIECEVCERVM----------------------------------------DEVIQ-----LAFSNHSQQVVSNFIENKI------CPRLG-FVKTECTSLIDTYLPVIFSLARSTLNSKVICAELKLCSLI-------------------SQKKINLLMKSNINDDSAK----------CKICHFVVSELLTLLTNPDIQKSIENFLEN--HVCPHFGAKKQECINDINQYYSLVINIITEFLKP-TLCNELRLCNSTTTSIIMSQPLVKMSRAILSSNCQLCEKFYFHLQKIFLKNTPISPLT-VFANVCNLEDTDETNECLKHFRNLNSIVAAFHASRNIGEF-CKMTEMCKGSKKHHVGKKQCSWGPGFWCISRQHAAGCGPGAVKHCEMKVW 2563
P K + CD C + +++ + D A + I +L+ + C + + + QC+++VD ++ +++ + EL D VC MG+C ++ S + +V E++ DEV Q L + + S+FI + I C LG + C + Y PV+ S + K ICA CS + S + L + + +AK C IC FV+ ++ +LT+ ++ + ++ ++ P + +C + I Y +I ++ + + P T+C L LCN + + +++ + R C++C+ ++ I KN + + VC+ EC + ++ +F C C +++ +G++QC+WGPGFWC + + A C AV HC VW
Sbjct: 54 PQMKSVPCDMCKEVLMVVEQLLKDNATEADILKYLE-KACQLIPDQGLTEQCKEMVDSYYPVLIGIITGELEDAGVVCAAMGLCKSQQASLAKLQVKEQLTSNDIPQVDLSHQAAPFLLNIPGLLYPQNLKEKQEVPQQGGDEVCQDCIKFLTDAQAEAKANSSFINSLIQNIEGQCDLLGPGLSDMCKQYVGQYGPVVVQQLMS-MQPKQICAMAGFCSEVKKSVPMMTLQAAKTIPAAKSIPALELFPATKVEAAAAKRLVRVKESPQCAICEFVMKQVEAMLTDETTEEEVIQAVQKVCYLVPS--SLTAQCKDLIETYGEAIIELLVQEVDPKTVCTMLALCNDASRAYVVA---LDQPRFKTGGYCEVCKMAVGYIDGILEKNATEAEIEEAVKKVCSFLPDAYKTECDQLVEQYEPVLVQLLLQMLDPDFVCTKVGACPEAERKLLGREQCTWGPGFWCKNMETANMC--NAVAHCRRHVW 531 HSP 2 Score: 68.9366 bits (167), Expect = 1.496e-11 Identity = 102/477 (21.38%), Postives = 191/477 (40.04%), Query Frame = 2
Query: 491 ICGLVQVCKNTNAMSFSKIFNSIKFQNDDVCTKCKQVFQFLESMF--SKVSPDEFKFLIESLICNQLPSE-VQSTCKLLLDGYVTQLYNLIKSLL-DGDRICPLIHLCSSENTQITLGSWQCQTCLDVIVVLRKDLAGVSAPEIVS----LIKSNVCSKLNFFEKG----------------------------------------------ICNSYVSNLVPTFVNELHSTLTPQQICGNHHVCT---------SHSIKKPIASEKGVIECDL---CEIGVTGAKSFISKPEN---------MNKVKSFIKS------------KICSHLKS-ISSQCSSLVDAYADQLITMISNHMDSKAICIAINACKGFSISQRKS--APNFK-GIECDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIF-QSEKIQCQQLVDEFFDIILNFVLSELDVDKVCKVMGMC--SMKKVSGIE 1639
+CG V C+ ++N + ++ C CK+V +E + + D K+L ++ C +P + + CK ++D Y L +I L D +C + LC S+ Q +L Q + L + + DL+ +AP +++ L N+ K ++G +C YV P V +L S + P+QIC C+ + K I + K + +L ++ AK + E+ M +V++ + K+C + S +++QC L++ Y + +I ++ +D K +C + C S + + P FK G C+ C +AV I + A + I+ +K ++C + K +C QLV+++ +++ +L LD D VC +G C + +K+ G E
Sbjct: 39 LCGAVAHCQQN-------VWNKPQMKSVP-CDMCKEVLMVVEQLLKDNATEADILKYLEKA--CQLIPDQGLTEQCKEMVDSYYPVLIGIITGELEDAGVVCAAMGLCKSQ--QASLAKLQVKEQLTSNDIPQVDLSHQAAPFLLNIPGLLYPQNLKEKQEVPQQGGDEVCQDCIKFLTDAQAEAKANSSFINSLIQNIEGQCDLLGPGLSDMCKQYVGQYGPVVVQQLMS-MQPKQICAMAGFCSEVKKSVPMMTLQAAKTIPAAKSIPALELFPATKVEAAAAKRLVRVKESPQCAICEFVMKQVEAMLTDETTEEEVIQAVQKVCYLVPSSLTAQCKDLIETYGEAIIELLVQEVDPKTVCTMLALCNDASRAYVVALDQPRFKTGGYCEVCKMAVGYIDGILEKNATEAEIEEAVK-KVCSFLPDAYKTECDQLVEQYEPVLVQLLLQMLDPDFVCTKVGACPEAERKLLGRE 501
BLAST of Proactivator polypeptide vs. Ensembl Medaka
Match: sftpbb (prosaposin-like [Source:NCBI gene;Acc:101161410]) HSP 1 Score: 71.2478 bits (173), Expect = 9.988e-13 Identity = 72/289 (24.91%), Postives = 124/289 (42.91%), Query Frame = 2
Query: 1727 NKICPRLGFVK-TECTSLIDTYLPVIFSLARSTLNSKVICAELKLCSLISQKK-------INLLMKSNINDDSAK---------CKICHFVVSELLTLLTNPDIQKSIENFLENHVCPHFGAK-KQECINDINQYYSLVINIITEFLKP-TLCNELRLCNSTTTSIIMSQPLVKMSRAILSSNCQLCEKFY-FHLQKIFLKNTPISPLTVFANVCNLEDTDETNECLKHFRNLNSIVAAFHASR-NIGEFCKMTEMCKGSKKHHV-GKKQCSWGPGFWCISRQHAAGCG 2527
+ +C RL + ++C S + TYLP + S LN C LC+ ++ +N SN SA C +C V+ +L T+L + +I +E VC A KQ+C + +N+Y + + + P T+C L LC + QP+ ++ +L S+C+ C H + L T +VC++ + +C R S + ++ +I C+ ++C+ KK + G C+WGP +WC +R+ A CG
Sbjct: 45 HAVCRRLPERQASDCQSQVKTYLPKVLQQKPSHLNPSETCVVFGLCAPQKDEELQEPPPSVNSEALSNSAPASATETQKISGPVCALCLMVIRKLETMLPKNISEDAILKIMEE-VCDLVPASYKQQCDDFVNKYGADIAEFLFSSAAPHTICMVLHLC------LFKEQPVPEV---LLPSDCESCRTLAALHRVHLGLNATGAQTSAFLQSVCSIH-PNAIPKCDAFTRAYGSQLHRVLGNQMDISHTCERADLCQTMKKLDLLGSNPCTWGPSYWCQNRETAQKCG 322 HSP 2 Score: 52.7582 bits (125), Expect = 9.957e-7 Identity = 60/290 (20.69%), Postives = 107/290 (36.90%), Query Frame = 2
Query: 548 FNSIKFQNDDVCTKCKQVFQFLESMFSKVSPDEFKFLIESLICNQLPSEVQSTCKLLLDGYVTQLYNLIKSLLDGDRICPLIHLC--------------------------SSENTQITLGSWQCQTCLDVIVVLRKDLAGVSAPEIVSLIKSNVCSKLNFFEKGICNSYVSNLVPTFVNELHSTLTPQQICGNHHVCTSHSIKKPIASEKGVIECDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHLKSISSQCSSLVDAYADQLITMISNHMDSKAICIAINACK 1339
F + + Q+ D+C +C Q+ + +M S + +C +LP S C+ + Y+ ++ S L+ C + LC S+ TQ G C CL VI L L + + + I VC + K C+ +V+ L S+ P IC H+C ++P+ +C+ C + + + +F++S +CS + +C + AY QL ++ N MD C + C+
Sbjct: 6 FITQELQSLDLCGECSQIIRTSTNMISGSDTKVTVYEALHAVCRRLPERQASDCQSQVKTYLPKVLQQKPSHLNPSETCVVFGLCAPQKDEELQEPPPSVNSEALSNSAPASATETQKISGPV-CALCLMVIRKLETMLPKNISEDAILKIMEEVCDLVPASYKQQCDDFVNKYGADIAEFLFSSAAPHTICMVLHLCLFK--EQPVPEVLLPSDCESCRTLAALHRVHLGLNATGAQTSAFLQS-VCSIHPNAIPKCDAFTRAYGSQLHRVLGNQMDISHTCERADLCQ 291 HSP 3 Score: 51.9878 bits (123), Expect = 1.663e-6 Identity = 50/237 (21.10%), Postives = 97/237 (40.93%), Query Frame = 2
Query: 287 DYCDACMKFVQSVEAHIPKISEQEFVNLAGNLICNNLKGKSQLDCKKEVNFFAPLIYRELSMEATPEKICGLVQVCK--------------NTNAMSFSKIFNSIKFQ--NDDVCTKCKQVFQFLESMFSK-VSPDEFKFLIESLICNQLPSEVQSTCKLLLDGYVTQLYNLIKSLLDGDRICPLIHLCSSENTQI--TLGSWQCQTCLDVIVVLRKDLAGVSAPEIVSLIKSNVCS 940
D C C + +++ I + V A + +C L + DC+ +V + P + ++ P + C + +C N+ A+S S ++ + Q + VC C V + LE+M K +S D ++E +C+ +P+ + C ++ Y + + S IC ++HLC + + L C++C + + R L + S +VCS
Sbjct: 15 DLCGECSQIIRTSTNMISGSDTKVTVYEALHAVCRRLPERQASDCQSQVKTYLPKVLQQKPSHLNPSETCVVFGLCAPQKDEELQEPPPSVNSEALSNSAPASATETQKISGPVCALCLMVIRKLETMLPKNISEDAILKIMEE-VCDLVPASYKQQCDDFVNKYGADIAEFLFSSAAPHTICMVLHLCLFKEQPVPEVLLPSDCESCRTLAALHRVHLGLNATGAQTSAFLQSVCS 250
BLAST of Proactivator polypeptide vs. Ensembl Medaka
Match: sftpbb (prosaposin-like [Source:NCBI gene;Acc:101161410]) HSP 1 Score: 71.2478 bits (173), Expect = 1.258e-12 Identity = 72/289 (24.91%), Postives = 124/289 (42.91%), Query Frame = 2
Query: 1727 NKICPRLGFVK-TECTSLIDTYLPVIFSLARSTLNSKVICAELKLCSLISQKK-------INLLMKSNINDDSAK---------CKICHFVVSELLTLLTNPDIQKSIENFLENHVCPHFGAK-KQECINDINQYYSLVINIITEFLKP-TLCNELRLCNSTTTSIIMSQPLVKMSRAILSSNCQLCEKFY-FHLQKIFLKNTPISPLTVFANVCNLEDTDETNECLKHFRNLNSIVAAFHASR-NIGEFCKMTEMCKGSKKHHV-GKKQCSWGPGFWCISRQHAAGCG 2527
+ +C RL + ++C S + TYLP + S LN C LC+ ++ +N SN SA C +C V+ +L T+L + +I +E VC A KQ+C + +N+Y + + + P T+C L LC + QP+ ++ +L S+C+ C H + L T +VC++ + +C R S + ++ +I C+ ++C+ KK + G C+WGP +WC +R+ A CG
Sbjct: 69 HAVCRRLPERQASDCQSQVKTYLPKVLQQKPSHLNPSETCVVFGLCAPQKDEELQEPPPSVNSEALSNSAPASATETQKISGPVCALCLMVIRKLETMLPKNISEDAILKIMEE-VCDLVPASYKQQCDDFVNKYGADIAEFLFSSAAPHTICMVLHLC------LFKEQPVPEV---LLPSDCESCRTLAALHRVHLGLNATGAQTSAFLQSVCSIH-PNAIPKCDAFTRAYGSQLHRVLGNQMDISHTCERADLCQTMKKLDLLGSNPCTWGPSYWCQNRETAQKCG 346 HSP 2 Score: 53.1434 bits (126), Expect = 8.426e-7 Identity = 60/290 (20.69%), Postives = 107/290 (36.90%), Query Frame = 2
Query: 548 FNSIKFQNDDVCTKCKQVFQFLESMFSKVSPDEFKFLIESLICNQLPSEVQSTCKLLLDGYVTQLYNLIKSLLDGDRICPLIHLC--------------------------SSENTQITLGSWQCQTCLDVIVVLRKDLAGVSAPEIVSLIKSNVCSKLNFFEKGICNSYVSNLVPTFVNELHSTLTPQQICGNHHVCTSHSIKKPIASEKGVIECDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHLKSISSQCSSLVDAYADQLITMISNHMDSKAICIAINACK 1339
F + + Q+ D+C +C Q+ + +M S + +C +LP S C+ + Y+ ++ S L+ C + LC S+ TQ G C CL VI L L + + + I VC + K C+ +V+ L S+ P IC H+C ++P+ +C+ C + + + +F++S +CS + +C + AY QL ++ N MD C + C+
Sbjct: 30 FITQELQSLDLCGECSQIIRTSTNMISGSDTKVTVYEALHAVCRRLPERQASDCQSQVKTYLPKVLQQKPSHLNPSETCVVFGLCAPQKDEELQEPPPSVNSEALSNSAPASATETQKISGP-VCALCLMVIRKLETMLPKNISEDAILKIMEEVCDLVPASYKQQCDDFVNKYGADIAEFLFSSAAPHTICMVLHLCLFK--EQPVPEVLLPSDCESCRTLAALHRVHLGLNATGAQTSAFLQS-VCSIHPNAIPKCDAFTRAYGSQLHRVLGNQMDISHTCERADLCQ 315 HSP 3 Score: 51.9878 bits (123), Expect = 1.587e-6 Identity = 50/237 (21.10%), Postives = 97/237 (40.93%), Query Frame = 2
Query: 287 DYCDACMKFVQSVEAHIPKISEQEFVNLAGNLICNNLKGKSQLDCKKEVNFFAPLIYRELSMEATPEKICGLVQVCK--------------NTNAMSFSKIFNSIKFQ--NDDVCTKCKQVFQFLESMFSK-VSPDEFKFLIESLICNQLPSEVQSTCKLLLDGYVTQLYNLIKSLLDGDRICPLIHLCSSENTQI--TLGSWQCQTCLDVIVVLRKDLAGVSAPEIVSLIKSNVCS 940
D C C + +++ I + V A + +C L + DC+ +V + P + ++ P + C + +C N+ A+S S ++ + Q + VC C V + LE+M K +S D ++E +C+ +P+ + C ++ Y + + S IC ++HLC + + L C++C + + R L + S +VCS
Sbjct: 39 DLCGECSQIIRTSTNMISGSDTKVTVYEALHAVCRRLPERQASDCQSQVKTYLPKVLQQKPSHLNPSETCVVFGLCAPQKDEELQEPPPSVNSEALSNSAPASATETQKISGPVCALCLMVIRKLETMLPKNISEDAILKIMEE-VCDLVPASYKQQCDDFVNKYGADIAEFLFSSAAPHTICMVLHLCLFKEQPVPEVLLPSDCESCRTLAALHRVHLGLNATGAQTSAFLQSVCS 274
BLAST of Proactivator polypeptide vs. Planmine SMEST
Match: SMESG000042333.1 (SMESG000042333.1) HSP 1 Score: 1673.68 bits (4333), Expect = 0.000e+0 Identity = 819/826 (99.15%), Postives = 824/826 (99.76%), Query Frame = 2
Query: 101 MRFLIFGFLISVCYAFDFDCLKGPTYWCATYQNAEKCDRITECFMGQGDQKNKVDISHHKNGDYCDACMKFVQSVEAHIPKISEQEFVNLAGNLICNNLKGKSQLDCKKEVNFFAPLIYRELSMEATPEKICGLVQVCKNTNAMSFSKIFNSIKFQNDDVCTKCKQVFQFLESMFSKVSPDEFKFLIESLICNQLPSEVQSTCKLLLDGYVTQLYNLIKSLLDGDRICPLIHLCSSENTQITLGSWQCQTCLDVIVVLRKDLAGVSAPEIVSLIKSNVCSKLNFFEKGICNSYVSNLVPTFVNELHSTLTPQQICGNHHVCTSHSIKKPIASEKGVIECDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHLKSISSQCSSLVDAYADQLITMISNHMDSKAICIAINACKGFSISQRKSAPNFKGIECDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIFQSEKIQCQQLVDEFFDIILNFVLSELDVDKVCKVMGMCSMKKVSGIECEVCERVMDEVIQLAFSNHSQQVVSNFIENKICPRLGFVKTECTSLIDTYLPVIFSLARSTLNSKVICAELKLCSLISQKKINLLMKSNINDDSAKCKICHFVVSELLTLLTNPDIQKSIENFLENHVCPHFGAKKQECINDINQYYSLVINIITEFLKPTLCNELRLCNSTTTSIIMSQPLVKMSRAILSSNCQLCEKFYFHLQKIFLKNTPISPLTVFANVCNLEDTDETNECLKHFRNLNSIVAAFHASRNIGEFCKMTEMCKGSKKHHVGKKQCSWGPGFWCISRQHAAGCGPGAVKHCEMKVWNQMPN 2578
MRFLIFGFLISVCYAFDFDCLKGPTYWCATYQNAEKCDRITECFMGQGDQKNKVDISHHKNGDYCDACMKFVQSVEAHIPKISEQEFVNLAGNLICNNLKGKSQLDCKKEVNFFAP+IYRELSMEATPEKICGLVQVCKNTNAMSFSKIFNSIKFQNDDVCTKCKQVFQFLESMFSKVSPDEFKFLIESLICNQLPSEVQSTCKLLLDGYVTQLYNLIKSLLDGDRICPLIHLCSSENT+ITLGSWQCQTCLDVIVVLRKDLAGVSAPEIVSLI+SNVCSKLNFFEKGICNSYVSNLVPTFVNELHS LTPQQICGNHHVCTSHSIKKPIASEKGVIECDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHLKSISSQCSSLVDAYADQLITMISNHMDSKAICIAINACKGFSISQRKSAPNFKGIECDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIFQSEKIQCQQLVDEFFDIILNF+LSELDVDKVCKVMGMCSMKKVSGIECEVCERVMDEVIQLAFSNHSQQVVSNFIENKICPRLGFVKTECTSLIDTYLPVIFSLARSTLNSKVICAELKLCSLISQKKINLLMKSNINDDSAKCKICHFVVSELLTLLTNPDIQKSIENFLENHVCPHFGAKKQECINDINQYYSLVINIITEFLKPTLCNELRLCNSTTTSIIMSQPLVKMSRAILSSNCQLCEKFYFHLQKIFLKNTPISPLTVFANVCNLEDTDETNECLKHFRNLNSIVAAFHASRNIGEFCKMT+MCK SKKHHVGKKQCSWGPGFWCISRQHAAGCGPGAVKHCEMKVWNQMPN
Sbjct: 1 MRFLIFGFLISVCYAFDFDCLKGPTYWCATYQNAEKCDRITECFMGQGDQKNKVDISHHKNGDYCDACMKFVQSVEAHIPKISEQEFVNLAGNLICNNLKGKSQLDCKKEVNFFAPIIYRELSMEATPEKICGLVQVCKNTNAMSFSKIFNSIKFQNDDVCTKCKQVFQFLESMFSKVSPDEFKFLIESLICNQLPSEVQSTCKLLLDGYVTQLYNLIKSLLDGDRICPLIHLCSSENTRITLGSWQCQTCLDVIVVLRKDLAGVSAPEIVSLIQSNVCSKLNFFEKGICNSYVSNLVPTFVNELHSILTPQQICGNHHVCTSHSIKKPIASEKGVIECDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHLKSISSQCSSLVDAYADQLITMISNHMDSKAICIAINACKGFSISQRKSAPNFKGIECDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIFQSEKIQCQQLVDEFFDIILNFILSELDVDKVCKVMGMCSMKKVSGIECEVCERVMDEVIQLAFSNHSQQVVSNFIENKICPRLGFVKTECTSLIDTYLPVIFSLARSTLNSKVICAELKLCSLISQKKINLLMKSNINDDSAKCKICHFVVSELLTLLTNPDIQKSIENFLENHVCPHFGAKKQECINDINQYYSLVINIITEFLKPTLCNELRLCNSTTTSIIMSQPLVKMSRAILSSNCQLCEKFYFHLQKIFLKNTPISPLTVFANVCNLEDTDETNECLKHFRNLNSIVAAFHASRNIGEFCKMTKMCKRSKKHHVGKKQCSWGPGFWCISRQHAAGCGPGAVKHCEMKVWNQMPN 826
BLAST of Proactivator polypeptide vs. Planmine SMEST
Match: SMESG000019975.1 (SMESG000019975.1) HSP 1 Score: 59.6918 bits (143), Expect = 2.026e-9 Identity = 54/208 (25.96%), Postives = 98/208 (47.12%), Query Frame = 2
Query: 1565 LSELDVDKVCKVMGMCSMKKVSGIECEVCERVMDEVIQLAFSNHSQQVVSNFIENKICPRLGFV-KTECTSLIDTYLPVIFSLARSTLNSKVICAELKLCSLISQKKINLLMKSNINDDSAKCKICHFVVSELLTLLTNPDIQKSIENFLENHVCPHFGA-KKQECINDINQYYSLVINIITEF-LKPTLCNELRLCNSTTTSIIMSQ 2179
+SE+++ K+ M + ++ C+VC++++ +VI SNHS+ VV +E K+C L FV K C ++D + I L + IC + LCS +L S + S C IC ++ E+ + + + I+ LE VC F + +C +++Y ++ +I E +C+ L+LC + I S+
Sbjct: 56 ISEIEIHAQIKI-KMTNSEENKSFGCDVCKKLIQQVIDAIGSNHSKDVVRKELE-KVCDGLPFVWKPVCKDIVDKFADTIVDEIVKNLPADKICQNIGLCS-------SLAAISPPREKSVLCWICSQIIEEIFKQIGERRVAEEIKRALEG-VCNIFPVIYRHQCNEFVDRYAVEIVKLILEGKAGGVICSALQLCETRNYPTIKSK 253 HSP 2 Score: 53.5286 bits (127), Expect = 2.600e-7 Identity = 45/178 (25.28%), Postives = 75/178 (42.13%), Query Frame = 2
Query: 293 CDACMKFVQSVEAHIPKISEQEFVNLAGNLICNNLKGKSQLDCKKEVNFFAPLIYRELSMEATPEKICGLVQVCKNTNAMSFSKIFNSIKFQNDDVCTKCKQVFQFLESMFSKVS----PDEFKFLIESLICNQLPSEVQSTCKLLLDGYVTQLYNLIKSLLDGDRICPLIHLCSSEN 814
CD C K +Q V I ++ V +C+ L + CK V+ FA I E+ +KIC + +C + A+S + + +C C Q+ +E +F ++ +E K +E +CN P + C +D Y ++ LI G IC + LC + N
Sbjct: 80 CDVCKKLIQQVIDAIGSNHSKDVVRKELEKVCDGLPFVWKPVCKDIVDKFADTIVDEIVKNLPADKICQNIGLCSSLAAISPPR-------EKSVLCWICSQI---IEEIFKQIGERRVAEEIKRALEG-VCNIFPVIYRHQCNEFVDRYAVEIVKLILEGKAGGVICSALQLCETRN 246 HSP 3 Score: 51.2174 bits (121), Expect = 1.583e-6 Identity = 37/168 (22.02%), Postives = 75/168 (44.64%), Query Frame = 2
Query: 1379 KGIECDACHLAVTKIKEYISDPARKTSIKNFLKMRICPIFQSEKIQCQQLVDEFFDIILNFVLSELDVDKVCKVMGMCSM------KKVSGIECEVCERVMDEVIQLAFSNHSQQVVSNFIENKICPRLGFV-KTECTSLIDTYLPVIFSLARSTLNSKVICAELKLC 1861
K CD C + ++ + I K ++ L+ + K C+ +VD+F D I++ ++ L DK+C+ +G+CS + + C +C ++++E+ + + + +E +C + + +C +D Y I L VIC+ L+LC
Sbjct: 76 KSFGCDVCKKLIQQVIDAIGSNHSKDVVRKELEKVCDGLPFVWKPVCKDIVDKFADTIVDEIVKNLPADKICQNIGLCSSLAAISPPREKSVLCWICSQIIEEIFKQIGERRVAEEIKRALEG-VCNIFPVIYRHQCNEFVDRYAVEIVKLILEGKAGGVICSALQLC 242 HSP 4 Score: 49.6766 bits (117), Expect = 4.994e-6 Identity = 42/170 (24.71%), Postives = 73/170 (42.94%), Query Frame = 2
Query: 833 SWQCQTCLDVIVVLRKDLAGVSAPEIVSLIKSNVCSKLNFFEKGICNSYVSNLVPTFVNELHSTLTPQQICGNHHVCTSHSIKKPIASEKGVIECDLCEIGVTGAKSFISKPENMNKVKSFIKSKICSHLKSI-SSQCSSLVDAYADQLITMISNHMDSKAICIAINACK 1339
S+ C C +I + + + ++V VC L F K +C V T V+E+ L +IC N +C+S + P EK V+ C +C + I + ++K ++ +C+ I QC+ VD YA +++ +I IC A+ C+
Sbjct: 77 SFGCDVCKKLIQQVIDAIGSNHSKDVVRKELEKVCDGLPFVWKPVCKDIVDKFADTIVDEIVKNLPADKICQNIGLCSSLAAISP-PREKSVL-CWICSQIIEEIFKQIGERRVAEEIKRALEG-VCNIFPVIYRHQCNEFVDRYAVEIVKLILEGKAGGVICSALQLCE 243 The following BLAST results are available for this feature:
BLAST of Proactivator polypeptide vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 4
BLAST of Proactivator polypeptide vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 2
BLAST of Proactivator polypeptide vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 2
BLAST of Proactivator polypeptide vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 3
BLAST of Proactivator polypeptide vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Proactivator polypeptide vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Proactivator polypeptide vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Proactivator polypeptide vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Proactivator polypeptide vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Proactivator polypeptide vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of Proactivator polypeptide vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Proactivator polypeptide vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 3
BLAST of Proactivator polypeptide vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 3
BLAST of Proactivator polypeptide vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 2
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30019668 ID=SMED30019668|Name=Proactivator polypeptide|organism=Schmidtea mediterranea sexual|type=transcript|length=2787bpback to top protein sequence of SMED30019668-orf-1 >SMED30019668-orf-1 ID=SMED30019668-orf-1|Name=SMED30019668-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=828bp MRFLIFGFLISVCYAFDFDCLKGPTYWCATYQNAEKCDRITECFMGQGDQback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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