SMED30019664

Overview
NameSMED30019664
Smed IDSMED30019664
Length (bp)1691
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30019664 (SMED30019664) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30019664 (SMED30019664) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30019664 (SMED30019664) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30019664 (SMED30019664) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30019664

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 27

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30019664h1SMcG0009890 SmedASXL_017257SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
head regionSMED30019664h1SMcG0009890 SmedASXL_004369SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
head regionSMED30019664h1SMcG0009890 SmedASXL_017252SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
Smed sexual biotypeSMED30019664h1SMcG0009889 Contig19558newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30019664h1SMcG0009889 Contig19558uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30019664h1SMcG0009890 Contig53784newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30019664h1SMcG0009890 Contig53784uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30019664h1SMcG0009890 Contig19558newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30019664h1SMcG0009890 Contig19558uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
nervous systemSMED30019664h1SMcG0009890 dd_Smed_v4_13603_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30019664h1SMcG0009890 dd_Smed_v4_13603_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30019664h1SMcG0009890 dd_Smed_v4_13603_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30019664h1SMcG0009890 dd_Smed_v4_11640_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30019664h1SMcG0009890 dd_Smed_v4_11640_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30019664h1SMcG0009890 dd_Smed_v4_9830_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30019664h1SMcG0009890 dd_Smed_v4_9830_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30019664h1SMcG0009890 dd_Smed_v4_9830_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30019664h1SMcG0009890 dd_Smed_v4_9830_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30019664h1SMcG0009890 dd_Smed_v4_9830_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30019664h1SMcG0009890 dd_Smed_v4_9830_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30019664h1SMcG0009890 dd_Smed_v4_16650_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30019664h1SMcG0009890 dd_Smed_v4_16650_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30019664h1SMcG0009890 dd_Smed_v4_16650_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30019664h1SMcG0009890 dd_Smed_v6_9830_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
neoblastSMED30019664h1SMcG0009890 dd_Smed_v6_16650_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
reproductive organSMED30019664h1SMcG0009890 Contig53784newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30019664h1SMcG0009890 Contig53784uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30019664 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000055:311950..315745 -8284
v31.000055:314302..316713 -4372
v31.000055:315382..317064 -3725
v31.000055:315615..317093 -2707
v31.000055:315521..317094 -2564
Homology
[Close]
BLAST of SMED30019664 vs. Planmine SMEST
Match: SMESG000064314.1 (SMESG000064314.1)

HSP 1 Score: 723.776 bits (1867), Expect = 0.000e+0
Identity = 487/638 (76.33%), Postives = 506/638 (79.31%), Query Frame = 2 
Query:    2 NSDEVIKVDVKDKSVNPDESELDEPNCIEIVEVDKNISEESNVKELLKVTETNHSVDQNEGELDEQIIIEIDKSEKNISDQVNVDELSKVDEIDHFADPXXXXXXXXXXXXXXKEETKQQELVNSKELSEVDEKDNSYNPNKKEFVEPNIKNVDEVEKNIIEESYLKALSMIDGTKHSVDENEEELAELNIKNVNEKERSLIYNANLIEVSKVDEIYYSEDPNENTLTEPITKEVDNIENIQTDYVKSEELKADETEYSVDQIERESAESNIDEVDEEEITQEKFNLKEVSKVDETDHSVDPNESEPSGSNINEIGQGEKNISDKVNVDQVSKDDESDHSVDPNKSEPGKSYINEIEGEITHKKINLDEVSKVDENNDSVDPXXXXXXXXXXXXXXXGKTXXXXXXXXXXXXTIDEPDLSVDPNKSELA-----------------------------------------ELNINGVDEEEPKQQQQNFNFEEASKV---------------------------HEK------------DNSDDPNENELVEPHIKNIVEGEKNISDESNVKELTKVFEKQHSFDQ----------IEGNSEERNITDDVNYKDVSHVDETDHADDSNESEPAESNFN 1645
            NSDEVI VDVKDKSVNPDESEL EPNCIEIVEVDKNISEESNVKELLKVTETNHSVDQNEGELDEQ IIEIDKSEKNISDQVNVDELSKVDEIDH ADPNESESANSNINEVNK+ETKQQELVNSKELSEV EKDNSYNPNKKEFVEPNIKNVDEVEKNIIEESYLKALSMIDGTKHSVDENEEELAELNIKNVNEKERSLIYNANLIEVSKVDEIYYSEDPNENTLTEPITKEVDNIENIQTDYVKSEELKADETEYSVDQIERESAESNIDEVDEEEITQEKFNLKEVSKVDETDHSVDPNESEPSGSNINEIGQGEKNISDKVNVDQVSKDDESDHSVDPNKSEPGKSYINEIEGEITHKKINLDEV KVDENNDSVDPNEIEIVESNIENVEEGKTNLSEESNINESSTIDEPDLSVDPNKSEL+                                         E NI  VD  EPKQ++Q  N EE SKV                           HEK            D+S DPNE E+V+P+IK + EGE NIS+ESNV EL+K+ E   S             IE +  + NITD VN  ++S VDE  H+ D NE+EP+E N N
Sbjct: 2367 NSDEVINVDVKDKSVNPDESELGEPNCIEIVEVDKNISEESNVKELLKVTETNHSVDQNEGELDEQNIIEIDKSEKNISDQVNVDELSKVDEIDHSADPNESESANSNINEVNKKETKQQELVNSKELSEVYEKDNSYNPNKKEFVEPNIKNVDEVEKNIIEESYLKALSMIDGTKHSVDENEEELAELNIKNVNEKERSLIYNANLIEVSKVDEIYYSEDPNENTLTEPITKEVDNIENIQTDYVKSEELKADETEYSVDQIERESAESNIDEVDEEEITQEKFNLKEVSKVDETDHSVDPNESEPSGSNINEIGQGEKNISDKVNVDQVSKDDESDHSVDPNKSEPGKSYINEIEGEITHKKINLDEVPKVDENNDSVDPNEIEIVESNIENVEEGKTNLSEESNINESSTIDEPDLSVDPNKSELSEHYIIEIDQVEKNISDPVNVDEVSKVEETDRTANLNESEPYESNIKEVDVGEPKQKEQ-VNLEEVSKVNENDYSRDPNGSEPDDSNINELGGKMTHEKFNLEEVSKARENDDSVDPNEREIVQPNIKCLDEGETNISEESNVNELSKIDEIDRSVYPNESKLAEHYIIEIDQSKNNITDPVNLNEISKVDEKGHSADLNENEPSEYNIN 3003

HSP 2 Score: 422.165 bits (1084), Expect = 1.102e-130
Identity = 329/643 (51.17%), Postives = 401/643 (62.36%), Query Frame = 2 
Query:    2 NSDEVIKVDVKDKSVNPDESELDEPNCIEIVEVDKNISEESNVKELLKVTETNHSVDQNEGELDEQIIIEIDKSEKNISDQVNVDELSKVDEIDHFADPXXXXXXXXXXXXXXKEETKQQELVNSKELSEVDEKDNSYNPNKKEFVEPNIKNVDEVEKNIIEESYLKALSMIDGTKH-----------------------------------------SVDENEEELAELNIKNVNEKERSLIYNANLIEVSKVDEIYYSEDPNENTLTEPITKEVDNIENIQTDYVKSEELKA-----------DETEYSVDQIERESAESNIDEVDEEEITQEKFNLKEVSKVDETDHSVDPNESEPSGSNINE-----------------------------------------IGQGEKNISDKVNVDQVSKDDESDHSVDPNKSEPGKSYINEI-EGEITHK-KINLDEVSKVDENNDSVDPXXXXXXXXXXXXXXXGKTXXXXXXXXXXXXTIDEPDLSVDPNKSELAELNINGVDEEEPKQQQQNFNFEEASKVHEKDNSDDPNENELVEPHIKNIVEGEKNISDESNVKELTKVFEKQHSFDQIEGNSEERNITDDVNYKDVSHVDETDHADDSNESEPAESNFN 1645
            N +E+ KVD K  S + +E+E  E N  E+ E +    E+ N + + KV E ++S D NE EL +  I EID+ E NI+D VNVDE+SKVDE  H AD  ESE +  NINEV++ E KQQE  N +E+ +V++KDNS +PN+ E VE +I  +D+ E NI +   +  +S ++ T H                                         S D NE E+ E NIKNV E E  +    N+ E+SK+DEI  S   NE+ L E    E+D  EN   D V  +E              +ET++S DQIERE AESNI+E  EEEITQEKFNL+++SKV E D S D NESEPS S INE                                         I QG+ NI+D VN++++SK DE DHS D N++EP +S INE+ EGE   + ++N + VSKV+E ++S DP+EIE  ESNI     G    +E+ N+ E+S I E D S DPNKSELAELNINGVDEEEPKQQQQNFNFEEASKVHEKDNSDDPNENELVEPHIKNIVEGEKNIS+ESNVKELTKVF KQHSFDQIEGNSEERNITDDVNYKDVSHVDETDHADDSNESEPAESNFN
Sbjct: 2976 NLNEISKVDEKGHSADLNENEPSEYNINEVDEGEPKQQEQVNPEGVSKVNEKDNSYDPNECELAQHNITEIDQGENNIADSVNVDEVSKVDEKYHSADSKESEPSEDNINEVDEGEPKQQEQFNPEEVLKVNKKDNSDDPNQSELVESHIIEIDQGENNIADHVNVDEVSKVEETDHTGNLNESEPYESNIKEVDEGEPKQQEQVNPEEVSKVNEKGDSGDTNESEIVEPNIKNVYEGETDISEEPNVNELSKIDEIDRSVYTNESKLAEHYIIEIDQGENNVADPVYVDEGSNFYGKDDSGDLNEETDHSADQIEREPAESNINEAGEEEITQEKFNLEDLSKVYEKDDSGDSNESEPSESYINEVDEGEPKQQEQVNPEEVSKVNEKDNSYDPNESGLAEYYIVEIDQGKNNITDPVNLNEISKVDEEDHSADLNENEPSESNINEVDEGEPKQQEQVNPEGVSKVNEKDNSNDPSEIEPAESNINEF--GGEMTNEKFNLVEASKIFEKDDSFDPNKSELAELNINGVDEEEPKQQQQNFNFEEASKVHEKDNSDDPNENELVEPHIKNIVEGEKNISEESNVKELTKVFGKQHSFDQIEGNSEERNITDDVNYKDVSHVDETDHADDSNESEPAESNFN 3616

HSP 3 Score: 307.76 bits (787), Expect = 3.546e-91
Identity = 292/638 (45.77%), Postives = 380/638 (59.56%), Query Frame = 2 
Query:    2 NSDEVIKVDVKDKSVNPDESELDEPNCIEIVEVDKNISEESNVKELLKVTETNHSVDQNEGELDEQIIIEIDKSEKNISDQVNVDELSKVDEIDHFADPXXXXXXXXXXXXXXKEETKQQELVNSKELSEVDEKDNSYNPNKKEFVEPNIKNVDEVEKNIIEESYLKALSMIDGTKHSVDENEEELAELNIKNVNEKERSLIYNANLIEVSKVDEIYYSEDPNENTLTEPITKEVDNIENIQTDYVKSEE-LKADETEYSVDQIERESAESNIDEVDEEEITQE-KFNLKEVSKVDETDHSVDPNESEPSGSNINEIGQGEKNISDKVNVDQVSKDDESDHSVDPNKSEPGKSYINEIEGEIT------------------------------------------HKKINLDEVSKVDENNDSVDPXXXXXXXXXXXXXXXGKTXXXXXXXXXXXXTIDEPDLSVDPNKSELAELNI------------------------------------NGVDEEEPKQQQQNFNFEEASKVHEKDNSDDPNENELVEPHIKNIVEGEKNISDESNVKELTKVFEKQHSFDQ----------IEGNSEERNITDDVNYKDVSHVDETDHADDSNESEPAESNFN 1645
            N DEV KVD  D+ V+P+ESE  E N  ++ E +    E+ N +E+  V E  +SV  NE  L E I  E+D  E   +   N +E SKVDE DH   PN+SE A SNIN+V++EETKQQ+ VNS+++ EV EK NS +PN  EF++PN+KNVDE EKNIIEES +K+LS +D  +HS D+ + ELAE NI+NV+E+ER++  +ANL E SKVD+I +S + NE TLTEPITKE+D  E  QTD+V SEE  K DE  YS D  E E AESNI+EVD+ E  Q+ K +L+EVS VD+TDHS D NES+ +  NI E+  GE+NIS++ N+++VS   E+D SVD N++EP +S IN+++ E T                                          HK+INLD VSK+DE + SVD NE + VESNI  V++G+    E+ N  E S +D+   SVD N+SELA  NI                                    N VD  EPKQQ+Q  N +E SKV  KD S D NE+EL EP++  I E EKNIS++SNVKEL  V E  HS DQ          IE +  E+NI D VN      VDETDH+ +SN+SEP+ES  N
Sbjct: 1518 NLDEVSKVDEIDQFVDPNESESAESNINDVDEGETKQQEQVNFEEVSNVYELENSVILNENTLTESIKKEVDNVENIQNVHANSEEESKVDETDHSIFPNQSEPAESNINKVDEEETKQQDQVNSEKVIEVVEKYNSDDPNYGEFIDPNMKNVDEEEKNIIEESNVKSLSNVDEKEHSTDQIKSELAEPNIRNVDEEERNITADANLKEESKVDKIDHSVNLNEKTLTEPITKEIDKNEQTQTDHVISEEKSKLDEKYYSADPNESEPAESNINEVDKAERKQQKKIDLEEVSNVDKTDHSADSNESKLAEQNIIEVDVGERNISEQDNLEEVSNVFETDPSVDLNENEPTESNINKVDEEETTQEQLISEEVSKVGEKDVSVDPNESDFVEHNIKKVDEDEKKQHKQINLD-VSKLDETDHSVDTNESKPVESNIIEVDDGEWTQQEQVNSEEVSNVDKTYHSVDQNESELANQNIIKIDKGEKNILDQVNVLYKTDHSVDPNKIKLAEFNINEVDNGEPKQQEQ-VNSDEVSKVDVKDKSVDTNESELCEPNVIEIDEVEKNISEKSNVKELLNVNETDHSDDQNDGELAKQIIIEIDEVEKNILDQVNV-----VDETDHSANSNKSEPSESKIN 2148

HSP 4 Score: 303.138 bits (775), Expect = 1.408e-89
Identity = 299/643 (46.50%), Postives = 376/643 (58.48%), Query Frame = 2 
Query:    2 NSDEVIKVDVKDKSVNPDESELDEPNCIEIVEVDKNISEESNVKELLKVTETNHSVDQNEGELDEQIIIEIDKSEKNISDQVNVDELSKVDEIDHFADPXXXXXXXXXXXXXXKEETKQQELVNSKELSEVDEKDNSYNPNKKEFVEPNIKNVDEVEKNIIEESYLKALSMIDGTKHSVDENEEELAELNIKNVNEKERSLIYNANLIEVSKVDEIYYSEDP-----------------------------------------NENTLTEPITKEVDNIENIQTDYVKS-EELKADETEYSVDQIERESAESNIDEVDEEEITQ------------------------------------------EKFNLKEVSKVDETDHSVDPNESEPSGSNINEIGQGEKNISDKVNVDQVSKDDESDHSVDPNKSEPGKSYINEIEGEITHKK--INLDEVSKVDENNDSVDPXXXXXXXXXXXXXXXGKTXXXXXXXXXXXXTIDEPDLSVDPNKSELAELNINGVDEEEPKQQQQNFNFEEASKVHEKDNSDDPNENELVEPHIKNIVEGEKNISDESNVKELTKVFEKQHSFDQIEGNSEERNI---------TDDVNYKDVSHVDETDHADDSNESEPAESNFN 1645
            NSDEV KVDVKDKSV+ +ESEL EPN IEI EV+KNISE+SNVKELL V ET+HS DQN+GEL +QIIIEID+ EKNI DQVNV     VDE DH A+ N+SE + S INEV++ + KQQ+ V+   +S+VD+KD S N N+ E  E NI  VDE E   +E+   + +S +D T HS + NE ELAE NI  V+  +R++    NL EVSKVDEI    DP                                         NENTLTEPI KEVDN+ENIQ  +  S EE K DET++S+   + E AESNI++VDEEE  Q                                          E+ N+KE+ KV ET+HSVD NE E    NI EI + EKNISD+VNVD++SK DE DHS DPN+SE   S INE+  + T ++  +N  E+S+V E ++S +PN+ E VE NI+NV+E + N+ EES +   S ID    SVD N+ ELAELNI  V+E+E +    N N  E SKV E   S+DPNEN L EP  K  V+  +NI  +    E  K  E ++S DQIE  S E NI          +  N K+VS VDETDH+ D NESEP+ SN N
Sbjct: 2044 NSDEVSKVDVKDKSVDTNESELCEPNVIEIDEVEKNISEKSNVKELLNVNETDHSDDQNDGELAKQIIIEIDEVEKNILDQVNV-----VDETDHSANSNKSEPSESKINEVDEADWKQQKKVDLDGVSKVDKKDRSINLNESELAESNISEVDERETKQLEQVNSEEVSNVDITDHSGEPNEHELAEQNIIEVDVDKRNISEKVNLDEVSKVDEIDQFVDPNESESAESNINDVDEGETKQQEQVNFEEVSNVFELENSVILNENTLTEPIKKEVDNVENIQNVHANSEEESKVDETDHSIFPNQSEPAESNINKVDEEETKQQEQVNSDEVINVDVKDKSVNPDESELGEPNCIEIVEVDKNISEESNVKELLKVTETNHSVDQNEGELDEQNIIEIDKSEKNISDQVNVDELSKVDEIDHSADPNESESANSNINEVNKKETKQQELVNSKELSEVYEKDNSYNPNKKEFVEPNIKNVDEVEKNIIEESYLKALSMIDGTKHSVDENEEELAELNIKNVNEKE-RSLIYNANLIEVSKVDEIYYSEDPNENTLTEPITKE-VDNIENIQTDYVKSEELKADETEYSVDQIERESAESNIDEVDEEEITQEKFNLKEVSKVDETDHSVDPNESEPSGSNIN 2679

HSP 5 Score: 251.906 bits (642), Expect = 4.894e-72
Identity = 248/612 (40.52%), Postives = 334/612 (54.58%), Query Frame = 2 
Query:    2 NSDEVIKVDVKDKSVNPDESELDEPNCIEIVEVDKNISEESNVKELLKVTETNHSVDQNEGELDEQIIIEIDKSEKNISDQVNVDELSKVDEIDHFADPXXXXXXXXXXXXXXKEETKQQELVNSKELSEVDEKDNSYNPNKKEFVEPNIKNVDEVEKNIIEESYLKALSMIDGTKHSVDENEEELAELNIKNVNEKERSLIYNANLIEVSKVDEIYYSEDPNENTLTEPITKEVDNIENIQTDYVKSEEL-KADETEYSVDQIERESAESNIDEVDEEEITQ------------------------------------------EKFNLKEVSKVDETDHSVDPNESEPSGSNINEIGQGEKNISDKVNVDQVS----KDD------ESDHSVDPNKSEPGKSYINEI-EGEITHKKINLDEVSKVDENNDSVDPXXXXXXXXXXXXXXXGKTXXXXXXXXXXXXTIDEPDLSVDPNKSELAELNINGVDEEEPKQQQQNFNFEEASKVHEKDNSDDPNENELVEPHIKNIVEGEKNISDESNVKELTKVFEKQHSFDQIEGNSEERNIT----------DDVNYKDVSHVDETDHADDSNESEPAESNFN 1645
            N +EV K    D SV+P+E E+ +PN   + E + NISEESNV EL K+ E + SV  NE +L E  IIEID+S+ NI+D VN++E+SKVDE  H AD NE+E +  NINEV++ E KQQE VN + +S+V+EKDNSY+PN+ E  + NI  +D+ E NI +   +  +S +D   HS D  E E +E NI  V+E E       N  EV KV++   S+DPN++ L E    E+D  EN   D+V  +E+ K +ET+++ +  E E  ESNI EVDE E  Q                                          E+ N+ E+SK+DE D SV  NES+ +   I EI QGE N++D V VD+ S    KDD      E+DHS D  + EP +S INE  E EIT +K NL+++SKV E +D                                         S D N+SE +E  IN VDE EPKQQ+Q  N EE SKV+EKDNS DPNE+ L E +I  I +G+ NI+D  N+ E++KV E+ HS D  E    E NI           + VN + VS V+E D+++D +E EPAESN N
Sbjct: 2894 NLEEVSKARENDDSVDPNEREIVQPNIKCLDEGETNISEESNVNELSKIDEIDRSVYPNESKLAEHYIIEIDQSKNNITDPVNLNEISKVDEKGHSADLNENEPSEYNINEVDEGEPKQQEQVNPEGVSKVNEKDNSYDPNECELAQHNITEIDQGENNIADSVNVDEVSKVDEKYHSADSKESEPSEDNINEVDEGEPKQQEQFNPEEVLKVNKKDNSDDPNQSELVESHIIEIDQGENNIADHVNVDEVSKVEETDHTGNLNESEPYESNIKEVDEGEPKQQEQVNPEEVSKVNEKGDSGDTNESEIVEPNIKNVYEGETDISEEPNVNELSKIDEIDRSVYTNESKLAEHYIIEIDQGENNVADPVYVDEGSNFYGKDDSGDLNEETDHSADQIEREPAESNINEAGEEEITQEKFNLEDLSKVYEKDD-----------------------------------------SGDSNESEPSESYINEVDEGEPKQQEQ-VNPEEVSKVNEKDNSYDPNESGLAEYYIVEIDQGKNNITDPVNLNEISKVDEEDHSADLNENEPSESNINEVDEGEPKQQEQVNPEGVSKVNEKDNSNDPSEIEPAESNIN 3463

HSP 6 Score: 239.58 bits (610), Expect = 7.549e-68
Identity = 274/684 (40.06%), Postives = 359/684 (52.49%), Query Frame = 2 
Query:    2 NSDEVIKVDVKDKSVNPDESELDEPNCIEIVEVDKNISEESNVKELLKVTETNHSVDQNEGELDEQIIIEIDKSEKNISDQVNVDELSKVDEIDHFADPXXXXXXXXXXXXXXKEETKQQELVNSKELSEVDEKDNSYNPNKKEFVEPNIKNVDEVEKNIIEESY-LKALSMIDGTKHSVDENEEELAELNIKNVNEKERSLIYNANLIEVSKVDEIYYSEDPNENTLTEPITKEVDNIEN-IQTDYVKSEEL-KADETEYSVDQIERESAESNIDEVDEEEI-TQEKFNLKEVSKVDETDHSVDPNESEPSGSNINEIGQGEKNISDKVNVDQVSKDDESD-----------------------------------------HSVDPNKSEPGKSYINEIEGEITHKKINLDEVSKVDENNDSVDPXXXXXXXXXXXXXXXGKTXXXXXXXXXXXXTIDEPDLSVDPNKSELA-----------------------------------------ELNINGVDEEEPKQQQQNFNFEEASKVHEKDNSDDPNENELVEPHIKNIVEGEKNISDESNVKELTKVFEKQHSFDQ---------------------------------------------------IEGNSEERNITDDVNYKDVSHVDETDHADDSNESEPAESNF 1642
            NS E+ +V  KD S NP++ E  EPN   + EV+KNI EES +K L  +  T HSVD+NE EL E  I  +++ E+++    N+ E+SKVDEI +  DPNE+        EV+  E  Q + V S+EL + DE + S +  ++E  E NI  VDE E  I +E + LK +S +D T HSVD NE E +  NI  + + E+++    N+ +VSK DE  +S DPN+   +EP    ++ IE  I    +  +E+ K DE   SVD  E E  ESNI+ V+E +    E+ N+ E S +DE D SVDPN+SE S   I EI Q EKNISD VNVD+VSK +E+D                                         +S DPN SEP  S INE+ G++TH+K NL+EVSK  EN+DSVDPNE EIV+ NI+ ++EG+TN+SEESN+NE S IDE D SV PN+S+LA                                         E NIN VDE EPKQQ+Q  N E  SKV+EKDNS DPNE EL + +I  I +GE NI+D  NV E++KV EK HS D                                                    IE +  E NI D VN  +VS V+ETDH  + NESEP ESN 
Sbjct: 2490 NSKELSEVYEKDNSYNPNKKEFVEPNIKNVDEVEKNIIEESYLKALSMIDGTKHSVDENEEELAELNIKNVNEKERSLIYNANLIEVSKVDEIYYSEDPNENTLTEPITKEVDNIENIQTDYVKSEEL-KADETEYSVDQIERESAESNIDEVDEEE--ITQEKFNLKEVSKVDETDHSVDPNESEPSGSNINEIGQGEKNISDKVNVDQVSKDDESDHSVDPNK---SEPGKSYINEIEGEITHKKINLDEVPKVDENNDSVDPNEIEIVESNIENVEEGKTNLSEESNINESSTIDEPDLSVDPNKSELSEHYIIEIDQVEKNISDPVNVDEVSKVEETDRTANLNESEPYESNIKEVDVGEPKQKEQVNLEEVSKVNENDYSRDPNGSEPDDSNINELGGKMTHEKFNLEEVSKARENDDSVDPNEREIVQPNIKCLDEGETNISEESNVNELSKIDEIDRSVYPNESKLAEHYIIEIDQSKNNITDPVNLNEISKVDEKGHSADLNENEPSEYNINEVDEGEPKQQEQ-VNPEGVSKVNEKDNSYDPNECELAQHNITEIDQGENNIADSVNVDEVSKVDEKYHSADSKESEPSEDNINEVDEGEPKQQEQFNPEEVLKVNKKDNSDDPNQSELVESHIIEIDQGENNIADHVNVDEVSKVEETDHTGNLNESEPYESNI 3166

HSP 7 Score: 228.409 bits (581), Expect = 4.560e-64
Identity = 256/569 (44.99%), Postives = 340/569 (59.75%), Query Frame = 2 
Query:    2 NSDEVIKVDVKDKSVNPDESELDEPNCIEIVEVDKNISEESNVKELLKVTETNHSVDQNEGELDEQIIIEIDKSEKNISDQVNVDELSKVDEIDHFADPXXXXXXXXXXXXXXKEETKQQELVNSKELSEVDEKDNSYNPNKKEFVEP------NIKNVDEVEKNIIEESYLKALSMIDGTKHSVDENEEELAELNIKNVNEKERSLIYNANLIEVSKVDEIYYSEDPNENTLTEPITKEVDNIEN--IQTDYVKSEELKADETEYSVDQIERESAESNIDEVDEEE--ITQEKFNLKEVSKVDETDHSVDPNESEPSGSNINEIGQGEKNISDKVNVDQVSKDDESDHSVDPNKSEPGKSYINEIEG--EITHKKINLDEVSKVDENNDSVDPXXXXXXXXXXXXXXXGKTXXXXXXXXXXXXTIDEPDLSVDPNKSELAELNINGVDEEEPKQQQQNFNFEEASKVHEKDNSDDPNENELVEPHIKNIVEGEKNISDESNVKELTKVFEKQHSFDQIEGNSEERNI--TDD--------VNYKDVSHVDETDHADDSNESEPAESNF 1642
            NS+E  K+D  D SV+ +ESEL E N  E+ E +    E+ N +E+  V  T+HS + NE EL EQ II +D  ++NIS++VN+DE+SKVDEID F DPNESESA SNIN+V++ ETKQQE VN +E+S V E +NS   N+    E       N++N+  V  N  EE      S +D T HS+  N+ E AE NI  V+E+E       N  +V +V E Y S+DPN     +P  K VD  E   I+   VKS     DE E+S DQI+ E AE NI  VDEEE  IT +  NLKE SKVD+ DHSV+ NE   +     EI + E+  +D V  ++ SK DE  +S DPN+SEP +S INE++       KKI+L+EVS VD+ + S D NE ++ E NI  V+ G+ N+SE+ N+ E S + E D SVD N++E  E NIN VDEEE  Q+Q     EE SKV EKD S DPNE++ VE +IK + E EK    + N+ +++K+ E  HS D  E    E NI   DD        VN ++VS+VD+T H+ D NESE A  N 
Sbjct: 1436 NSEENSKLDETDHSVDLNESELAESNISEVDEGETKQLEQVNSEEVSNVDITDHSGEPNEHELAEQNIIGVDVDKRNISEKVNLDEVSKVDEIDQFVDPNESESAESNINDVDEGETKQQEQVNFEEVSNVYELENSVILNENTLTESIKKEVDNVENIQNVHANSEEE------SKVDETDHSIFPNQSEPAESNINKVDEEETKQQDQVNSEKVIEVVEKYNSDDPNYGEFIDPNMKNVDEEEKNIIEESNVKSLS-NVDEKEHSTDQIKSELAEPNIRNVDEEERNITADA-NLKEESKVDKIDHSVNLNEKTLTEPITKEIDKNEQTQTDHVISEEKSKLDEKYYSADPNESEPAESNINEVDKAERKQQKKIDLEEVSNVDKTDHSADSNESKLAEQNIIEVDVGERNISEQDNLEEVSNVFETDPSVDLNENEPTESNINKVDEEETTQEQ--LISEEVSKVGEKDVSVDPNESDFVEHNIKKVDEDEKKQHKQINL-DVSKLDETDHSVDTNESKPVESNIIEVDDGEWTQQEQVNSEEVSNVDKTYHSVDQNESELANQNI 1993

HSP 8 Score: 226.868 bits (577), Expect = 1.613e-63
Identity = 258/643 (40.12%), Postives = 354/643 (55.05%), Query Frame = 2 
Query:    2 NSDEVIKVDVKDKSVNPDESELDEPNCIEIVEVDK-NISEESNVKELLKVTETNHSVDQNEGELDEQIIIEIDKSEKNISDQVNVDELS-----------------------------------------KVDEIDHFADPXXXXXXXXXXXXXXKEETKQQELVNSKELSEVDEKDNSYNPNKKEFVEPNIKNVDEVEKNIIEESYLKALSMIDGTKHSVDENEEELAELNIKNVNEKERSLIYNANLIEVSKVDEIYYSEDPNENTLTEPITKEVDNIENIQTDYVKSEEL-KADETEYSVDQIERESAESNIDEVDEEE--ITQEKFNLKEVSKVDETDHSVDPNESEPSGSNINEIGQGEKNISDKVNVDQVSKDDESDHSVDPNK---SEP-------------------------------------GKSYINEI-EGEITHKKINLDEVSKVDENNDSVDPXXXXXXXXXXXXXXXGKTXXXXXXXXXXXXTIDEPDLSVDPNKSELAELNINGVDEEEPKQQQQNFNFEEASKVHEKDNSDDPNENELVEPHIKNIVEGEKNISDESNVKELTKVFEKQHSFDQ----------IEGNSEERNITDDVNYKDVSHVDETDHADDSNESEPAESNF 1642
            NS+EV  VD+ D S  P+E EL E N IE V+VDK NISE+ N+ E+ KV E +  VD NE E  E  I ++D+ E    +QVN +E+S                                         KVDE DH   PN+SE A SNIN+V++EETKQQE VNS E+  VD KD S NP++ E  EPN   + EV+KNI EES +K L  +  T HSVD+NE EL E NI  +++ E+++    N+ E+SKVDEI +S DPNE+        EV+  E  Q + V S+EL +  E + S +  ++E  E NI  VDE E  I +E + LK +S +D T HSVD NE E +  NI  + + E+++    N+ +VSK DE  +S DPN+   +EP                                      +S I+E+ E EIT +K NL EVSKVDE + SVDPNE E   SNI  + +G+ N+S++ N+++ S  DE D SVDPNKSE  +  IN +   E +   +  N +E  KV E ++S DPNE E+VE +I+N+ EG+ N+S+ESN+ E + + E   S D           IE +  E+NI+D VN  +VS V+ETD   + NESEP ESN 
Sbjct: 2203 NSEEVSNVDITDHSGEPNEHELAEQNIIE-VDVDKRNISEKVNLDEVSKVDEIDQFVDPNESESAESNINDVDEGETKQQEQVNFEEVSNVFELENSVILNENTLTEPIKKEVDNVENIQNVHANSEEESKVDETDHSIFPNQSEPAESNINKVDEEETKQQEQVNSDEVINVDVKDKSVNPDESELGEPNCIEIVEVDKNISEESNVKELLKVTETNHSVDQNEGELDEQNIIEIDKSEKNISDQVNVDELSKVDEIDHSADPNESESANSNINEVNKKETKQQELVNSKELSEVYEKDNSYNPNKKEFVEPNIKNVDEVEKNIIEESY-LKALSMIDGTKHSVDENEEELAELNIKNVNEKERSLIYNANLIEVSKVDEIYYSEDPNENTLTEPITKEVDNIENIQTDYVKSEELKADETEYSVDQIERESAESNIDEVDEEEITQEKFNLKEVSKVDETDHSVDPNESEPSGSNINEIGQGEKNISDKVNVDQVSKDDESDHSVDPNKSEPGKSYINEI---EGEITHKKINLDEVPKVDENNDSVDPNEIEIVESNIENVEEGKTNLSEESNINESSTIDEPDLSVDPNKSELSEHYIIEIDQVEKNISDPVNVDEVSKVEETDRTANLNESEPYESNI 2840

HSP 9 Score: 224.172 bits (570), Expect = 1.384e-62
Identity = 244/601 (40.60%), Postives = 337/601 (56.07%), Query Frame = 2 
Query:    2 NSDEVIKVDVKDKSVNPDESELDEPNCIEIVEVDKNISEESNVKELLKVTETNHSVDQNEGELDEQIIIEIDKSEKNISDQVNVDELSKVDEIDHFADPXXXXXXXXXXXXXXKEETKQQELVNSKELSEVDEKDNSYNPNKKEFVEPNIKNVDEVEKNIIEESYLKALSMIDGTKHSVDENEEELAELNIKNVNEKERSLIYNANLIEVSKVDEIYYSEDPNENTLTEPITKEVDNIENIQTDYVKS-EELKADETEYSVDQIERESAESNIDEVDEEEITQE-KFNLKEVSKVDETDHSVDPNESEPSGSNINEIGQGEKNISDKVNVDQVSKDDESDHSVDPNKS---EPGKSYINEIEGEITHKKINLDEVSKVDENNDSVDPXXXXXXXXXXXXXXXGKTXXXXXXXXXXXXTIDEPDLSVDPNKSELAELNINGVDEEEPKQQQQNFNFEEASKVHEKDNSDDPNENELVEPHIKNIVEGEKNISDESNVKELTKVFEKQHSFDQIEGNSEERNI--------------------------------TDDVNYK-----------------DVSHVDETDHADDSNESEPAESNF 1642
            N +EV KVD  ++ +NP+ SEL + N I++ E  K   E+ N++E+  V E ++SVDQNE  L E I+ E+   E     +VN +E SK+DE DH  D NESE A SNI+EV++ ETKQ E VNS+E+S VD  D+S  PN+ E  E NI  VD  ++NI E+  L  +S +D     VD NE E AE NI +V+E E       N  EVS V E+  S   NENTLTE I KEVDN+ENIQ  +  S EE K DET++S+   + E AESNI++VDEEE  Q+ + N ++V +V E  +S DPN  E    N+  + + EKNI ++ NV  +S  DE +HS D  KS   EP    ++E E  IT    NL E SKVD+ + SV+ NE  + E   + +++ +   ++     E S +DE   S DPN+SE AE NIN VD+ E KQQ++  + EE S V + D+S D NE++L E +I  +  GE+NIS++ N++E++ VFE   S D  E    E NI                                +D V +                  DVS +DETDH+ D+NES+P ESN 
Sbjct: 1355 NLEEVPKVDETEEFINPNRSELADHNIIKVEEETKK-QEQINLEEVTNVDERDYSVDQNEITLTEPIMKEVYNVENIQIQRVNSEENSKLDETDHSVDLNESELAESNISEVDEGETKQLEQVNSEEVSNVDITDHSGEPNEHELAEQNIIGVDVDKRNISEKVNLDEVSKVDEIDQFVDPNESESAESNINDVDEGETKQQEQVNFEEVSNVYELENSVILNENTLTESIKKEVDNVENIQNVHANSEEESKVDETDHSIFPNQSEPAESNINKVDEEETKQQDQVNSEKVIEVVEKYNSDDPNYGEFIDPNMKNVDEEEKNIIEESNVKSLSNVDEKEHSTDQIKSELAEPNIRNVDEEERNIT-ADANLKEESKVDKIDHSVNLNEKTLTEPITKEIDKNEQTQTDHVISEEKSKLDEKYYSADPNESEPAESNINEVDKAERKQQKK-IDLEEVSNVDKTDHSADSNESKLAEQNIIEVDVGERNISEQDNLEEVSNVFETDPSVDLNENEPTESNINKVDEEETTQEQLISEEVSKVGEKDVSVDPNESDFVEHNIKKVDEDEKKQHKQINLDVSKLDETDHSVDTNESKPVESNI 1952

HSP 10 Score: 224.172 bits (570), Expect = 1.520e-62
Identity = 238/553 (43.04%), Postives = 320/553 (57.87%), Query Frame = 2 
Query:    2 NSDEVIKVDVKDKSVNPDESELDEPNCIEIVEVDKNISEESNVKELLKVTETNHSVDQNEGELDEQIIIEIDKSEKNISDQVNVDELSKV----------DEIDHFADPXXXXXXXXXXXXXXKEETKQQELVNSKELSEVDEKDNSYNPNKKEFVEPNIKNVDEVEKNIIEESYLKALSMIDGTKHSVDENEEELAELNIKNVNEKERSLIYNANLIEVSKVDEIYYSEDPNENTLTEPITKEVDNIENIQTDYVKSEEL-KADETEYSVDQIERESAESNIDEVDEEEITQEKFNLKEVSKVDETDHSVDPNESEPSGSNINEIGQGE-KNISDKVNVDQVSKDDESDHSVDPNKSEPGKSYI-NEIEGEI-THKKINLDEVSKVDENNDSVDPXXXXXXXXXXXXXXXGKTXXXXXXXXXXXXTIDEPDLSVDPNKSELAELNINGVDEEEPKQQQQNFNFEEASKVHEKDNSDDPNENELVEPHIKNIVEGEKNISDESNVKELTKVFEKQHSFDQIEGNSEERNITDDVNYKDVSHVDETDHADDSNE 1618
            N +EV KV+ K  S + +ESE+ EPN   + E + +ISEE NV EL K+ E + SV  NE +L E  IIEID+ E N++D V VDE S            +E DH AD  E E A SNINE  +EE  Q++  N ++LS+V EKD+S + N+ E  E  I  VDE E    E+   + +S ++   +S D NE  LAE  I  +++ + ++    NL E+SKVDE  +S D NEN  +E    EVD  E  Q + V  E + K +E + S D  E E AESNI+E   E +T EKFNL E SK+ E D S DPN+SE +  NIN + + E K      N ++ SK  E D+S DPN++E  + +I N +EGE    ++ N+ E++KV     S D  E    E NI +    K          + S +DE D + D N+SE AE N N VDEEE   ++        SKV EKDNSDDP +NELVEP IKN   GEKNIS ES +K+L KV + +HS +Q++   EERNITDD N +D+S VDE DH+ +SNE
Sbjct: 3181 NPEEVSKVNEKGDSGDTNESEIVEPNIKNVYEGETDISEEPNVNELSKIDEIDRSVYTNESKLAEHYIIEIDQGENNVADPVYVDEGSNFYGKDDSGDLNEETDHSADQIEREPAESNINEAGEEEITQEKF-NLEDLSKVYEKDDSGDSNESEPSESYINEVDEGEPKQQEQVNPEEVSKVNEKDNSYDPNESGLAEYYIVEIDQGKNNITDPVNLNEISKVDEEDHSADLNENEPSESNINEVDEGEPKQQEQVNPEGVSKVNEKDNSNDPSEIEPAESNINEFGGE-MTNEKFNLVEASKIFEKDDSFDPNKSELAELNINGVDEEEPKQQQQNFNFEEASKVHEKDNSDDPNENELVEPHIKNIVEGEKNISEESNVKELTKVFGKQHSFDQIEGNSEERNITDDVNYK----------DVSHVDETDHADDSNESEPAESNFNEVDEEEGNSEE-------ESKVDEKDNSDDPYKNELVEPLIKNFNVGEKNISGESCLKDLLKVDKTEHSVNQMKCKIEERNITDDANLRDLSQVDEIDHSVNSNE 3714

HSP 11 Score: 209.92 bits (533), Expect = 1.014e-57
Identity = 248/608 (40.79%), Postives = 335/608 (55.10%), Query Frame = 2 
Query:    8 DEVIKVDVKDKSVNPDESELDEPNCIEIVEVDKNISEESNVKELLKVTETNHSVDQNEGELDEQIIIEIDKSEKNISDQVNVDELSKVDEIDHFADPXXXXXXXXXXXXXXKEETKQQELVNSKELSEVDEKDNSYNPNKKEFVEP------NIKNVDEVEKNIIEESYLKALSMIDGTKHSVDENEEELAELNIKNVNEKERSLIYNANLIEVSKVDEIYYSEDPNENTLTEPITKEVDNIE-NIQTDYVKSEELKADETEYSVDQIERESAESNIDEVDEEEI-TQEKFNLKEVSKVDETDHSVDPNESEPSGSNINEIGQGEKNISDKVNVDQVSKDDESDHSVDPNKS---EPGKSYINEIEGEITHKKINLDEVSKVDENNDSVDPXXXXXXXXXXXXXXXGKTXXXXXXXXXXXXTIDEPDLSVDPNKSEL----------------------------------------AELNINGVDEEEPKQQQQNFNFEEASKVHEKDNSDDPNENELVEPHIKNIVEGEKNISDESNVKELTKVFEKQHSFD------------QIEGNSEERNITDDVNYKDVSHVDETDHADDSNESEPAESNF 1642
            D V KVD KD+S+N +ESEL E N  E+ E +    E+ N +E+  V  T+HS + NE EL EQ IIE+D  ++NIS++VN+DE+SKVDEID F DPNESESA SNIN+V++ ETKQQE VN +E+S V E +NS   N+    EP      N++N+  V  N  EE      S +D T HS+  N+ E AE NI  V+E+E       N  EV  VD    S +P+E+ L EP   E+  ++ NI  +    E LK  ET +SVDQ E E  E NI E+D+ E    ++ N+ E+SKVDE DHS DPNESE + SNINE+ + E    + VN  ++S+  E D+S +PNK    EP    ++E+E  I  +   L  +S +D    SVD NE E+ E NI+NV E + +L   +N+ E S +DE   S DPN++ L                                        AE NI+ VDEEE  Q++  FN +E SKV E D+S DPNE+E    +I  I +GEKNISD+ NV +++K  E  HS D            +IEG    + I    N  +V  VDE + + D NE E  ESN 
Sbjct: 2164 DGVSKVDKKDRSINLNESELAESNISEVDERETKQLEQVNSEEVSNVDITDHSGEPNEHELAEQNIIEVDVDKRNISEKVNLDEVSKVDEIDQFVDPNESESAESNINDVDEGETKQQEQVNFEEVSNVFELENSVILNENTLTEPIKKEVDNVENIQNVHANSEEE------SKVDETDHSIFPNQSEPAESNINKVDEEETKQQEQVNSDEVINVDVKDKSVNPDESELGEPNCIEIVEVDKNISEESNVKELLKVTETNHSVDQNEGELDEQNIIEIDKSEKNISDQVNVDELSKVDEIDHSADPNESESANSNINEVNKKETKQQELVNSKELSEVYEKDNSYNPNKKEFVEPNIKNVDEVEKNIIEESY-LKALSMIDGTKHSVDENEEELAELNIKNVNEKERSLIYNANLIEVSKVDEIYYSEDPNENTLTEPITKEVDNIENIQTDYVKSEELKADETEYSVDQIERESAESNIDEVDEEEITQEK--FNLKEVSKVDETDHSVDPNESEPSGSNINEIGQGEKNISDKVNVDQVSKDDESDHSVDPNKSEPGKSYINEIEGEITHKKI----NLDEVPKVDENNDSVDPNEIEIVESNI 2758

HSP 12 Score: 155.992 bits (393), Expect = 1.128e-39
Identity = 181/508 (35.63%), Postives = 265/508 (52.17%), Query Frame = 2 
Query:    2 NSDEVIKVDVKDKSVNPDESELDEPNCIEIVEVDKNISEESNVKELLKVTETNHSVDQNEGELDEQIIIEIDKSEKNISDQVNVDELSKVDEIDHFADPXXXXXXXXXXXXXXKEET----------------------------------------KQQELVNSKELSEVDEKDNSYNPNKKEFVEPNIKNVDEVEKNIIEESYLKALSMIDGTKHSVDENEEELAELNIKN-VNEKERSLI--------------YNANLIEV----------SKVDEIYYSEDPNENTLTEPITKEVDNIE-NIQTDYVKSEELKADETEYSVDQIERESAESNIDEVDEEEITQEKFNLKEVSKVDETDHSVDPNESEPSGSNINEIGQGEKNISDKVNVDQVSKDDESDHSVDPNKSEPGKSYINEI-EGEIT-HKKINLDEVSKVDENNDSVDPXXXXXXXXXXXXXXXGKTXXXXXXXXXXXXTIDEPDLSVDPNKSELAELNINGVDEEE 1321
            N +EV KV+ KD S +P+ES L E   +EI +   NI++  N+ E+ KV E +HS D NE E  E  I E+D+ E    +QVN + +SKV+E D+  DP+E E A SNINE   E T                                        +QQ+  N +E S+V EKDNS +PN+ E VEP+IKN+ E EKNI EES +K L+ + G +HS D+ E    E NI + VN K+ S +                +N  EV          SKVDE   S+DP +N L EP+ K  +  E NI  +    + LK D+TE+SV+Q++ +  E NI          +  NL+++S+VDE DHSV+ NE   +G N+ E+ +G+KN  +  N++++SK DE  HS +PN+SE  +S I E+ EGE    K++N +E + ++ N + VD                G+   +E  N+ E S +D+ D  VDP +S   + ++N V E E
Sbjct: 3354 NPEEVSKVNEKDNSYDPNESGLAEYYIVEIDQGKNNITDPVNLNEISKVDEEDHSADLNENEPSESNINEVDEGEPKQQEQVNPEGVSKVNEKDNSNDPSEIEPAESNINEFGGEMTNEKFNLVEASKIFEKDDSFDPNKSELAELNINGVDEEEPKQQQQNFNFEEASKVHEKDNSDDPNENELVEPHIKNIVEGEKNISEESNVKELTKVFGKQHSFDQIEGNSEERNITDDVNYKDVSHVDETDHADDSNESEPAESNFNEVDEEEGNSEEESKVDEKDNSDDPYKNELVEPLIKNFNVGEKNISGESCLKDLLKVDKTEHSVNQMKCKIEERNI---------TDDANLRDLSQVDEIDHSVNSNERTLTGDNVIEVDKGQKNQHEPANLEEMSKVDEIHHSANPNESELDESNIYEVDEGETNQQKQVNPNESTPIECNTNEVD---------------GGEKYQTEHVNLEEESMVDKIDNFVDPKESPFTKPDVNKVSEGE 3837

HSP 13 Score: 125.561 bits (314), Expect = 1.129e-29
Identity = 147/346 (42.49%), Postives = 199/346 (57.51%), Query Frame = 2 
Query:  617 NLIEVSKVDEIYYSEDPNENTLTEPITKEVDNIENIQTDYVKSEEL-KADETEYSVDQIERESAESNIDEVDEEEITQEKFNLKEVSKVDETDHSVDPNESEPSGSNINEIGQGEKNISDKVNVDQVSKDDESDHSVDPNKSEPGKSYINEI-EGEITH-KKINLDEVSKVDENNDSVDPXXXXXXXXXXXXXXXGKTXXXXXXXXXXXXTIDEPDLSVDPNKSELAELNINGVDEEEPKQQQQNFNFEEASKVHEKDNSDDPNENELVEPHIKNIVEGEKNISDESNVKELTKVFEKQHSFDQIEGNSEERNITDDVNYKDVSHVDETDHADDSNESEPAESNFN 1645
            NL  ++K+D+   S DP E+   E    ++D  E I+   V  EE+ K DETE  ++    E A+ NI +V+EE   QE+ NL+EV+ VDE D+SVD NE   +   + E+   E     +VN ++ SK DE+DHSVD N+SE  +S I+E+ EGE    +++N +EVS VD  + S +PNE E+ E NI  V+  K N+SE+ N++E S +DE D  VDPN+SE AE NIN VDE E KQQ+Q  NFEE S V+E +NS   NEN L E  IK  V+  +NI +                   +  NSEE            S VDETDH+   N+SEPAESN N
Sbjct: 1315 NLYVMTKMDQKDNSVDPYESAHIEAKINKIDE-ETIKQKQVNLEEVPKVDETEEFINPNRSELADHNIIKVEEETKKQEQINLEEVTNVDERDYSVDQNEITLTEPIMKEVYNVENIQIQRVNSEENSKLDETDHSVDLNESELAESNISEVDEGETKQLEQVNSEEVSNVDITDHSGEPNEHELAEQNIIGVDVDKRNISEKVNLDEVSKVDEIDQFVDPNESESAESNINDVDEGETKQQEQ-VNFEEVSNVYELENSVILNENTLTE-SIKKEVDNVENIQN-------------------VHANSEEE-----------SKVDETDHSIFPNQSEPAESNIN 1627

HSP 14 Score: 76.6406 bits (187), Expect = 3.083e-14
Identity = 90/277 (32.49%), Postives = 144/277 (51.99%), Query Frame = 2 
Query:   14 VIKVDVKDKSVNPDESELDEPNCIEIVEVDKNISEESNVKELLKVTETNHSVDQNEGELDEQIIIEIDKSEKNISDQVNVDELSKVDEIDHFADPXXXXXXXXXXXXXXKEETKQQELVNSKELSEVDEKDNSYNPNKKEFVEPNIKNVDEVEKNIIEESYLKALSMIDGTKHSVDENEEELAELNIKNVNEKERSLIYNANLIEVSKVDEIYYSEDPNENT---------LTEPITKEV-DNIENIQTDYVKSEELKADETEYSVDQIERESAESN 814
            ++KVD  + SVN  + +++E          +NI++++N+++L +V E +HSV+ NE  L    +IE+DK +KN  +  N++E+SKVDEI H A+PNESE   SNI EV++ ET QQ+ V               NPN+   +E N   VD  EK   E   L+  SM+D   + VD  E    + ++  V+E E++      +++ + VD I+   D  EN+         L  P    + DN  +++T+    EE+ +DE    VD+   E   SN
Sbjct: 3669 LLKVDKTEHSVNQMKCKIEE----------RNITDDANLRDLSQVDEIDHSVNSNERTLTGDNVIEVDKGQKNQHEPANLEEMSKVDEIHHSANPNESELDESNIYEVDEGETNQQKQV---------------NPNESTPIECNTNEVDGGEKYQTEHVNLEEESMVDKIDNFVDPKESPFTKPDVNKVSEGEKNK-AETQIVKSTDVDTIFVQNDVKENSKMYIENAQILASPNVISINDNKVDVKTNESSKEEIASDEDWIKVDEESTEKTNSN 3919
The following BLAST results are available for this feature:
BLAST of SMED30019664 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019664 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019664 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019664 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019664 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019664 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019664 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019664 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019664 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019664 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019664 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019664 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019664 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019664 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000064314.11.102e-13076.33SMESG000064314.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30019664 ID=SMED30019664|Name=SMED30019664|organism=Schmidtea mediterranea sexual|type=transcript|length=1691bp
CAATTCGGACGAAGTAATAAAGGTTGATGTAAAAGATAAGTCTGTTAATC
CTGATGAAAGTGAACTTGACGAGCCTAATTGCATTGAAATAGTTGAAGTT
GATAAAAATATATCAGAGGAATCAAATGTAAAAGAACTGTTAAAGGTTAC
TGAAACAAATCATTCCGTCGATCAAAATGAAGGTGAACTTGATGAGCAAA
TTATAATCGAAATCGATAAAAGTGAAAAGAATATATCAGATCAAGTTAAT
GTGGATGAGTTGTCAAAAGTTGATGAAATAGATCATTTTGCTGATCCAAA
TGAAAGTGAATCTGCTAACTCTAATATTAATGAAGTTAACAAGGAAGAAA
CAAAACAACAAGAGCTAGTTAATTCTAAAGAATTATCAGAGGTTGATGAA
AAGGATAATTCTTATAATCCTAATAAAAAAGAATTTGTTGAGCCTAATAT
CAAGAACGTGGACGAAGTAGAAAAGAATATTATCGAGGAATCTTATTTAA
AAGCACTGTCAATGATTGATGGAACAAAGCATTCTGTTGATGAAAATGAA
GAGGAGCTTGCTGAACTTAATATCAAGAACGTCAATGAAAAGGAAAGGAG
TTTGATCTATAATGCAAATTTAATAGAAGTGTCAAAAGTTGATGAAATAT
ATTATTCTGAAGATCCGAATGAAAATACGCTTACTGAGCCCATCACGAAA
GAAGTCGATAATATAGAAAACATTCAAACCGATTATGTAAAATCTGAAGA
GTTAAAAGCTGATGAAACAGAATATTCTGTCGATCAAATTGAACGTGAAT
CTGCTGAGTCTAATATCGATGAAGTCGACGAGGAAGAAATAACGCAAGAG
AAATTTAATTTAAAAGAAGTATCAAAGGTTGATGAAACAGATCATTCTGT
TGATCCAAATGAAAGTGAACCTTCTGGGTCTAATATCAATGAAATCGGTC
AAGGTGAAAAGAATATATCAGATAAAGTTAATGTAGATCAGGTGTCAAAG
GATGATGAATCAGATCATTCTGTTGATCCAAATAAAAGTGAACCTGGTAA
ATCTTATATCAATGAAATTGAAGGAGAAATTACACACAAAAAGATTAATT
TGGACGAGGTATCAAAAGTTGATGAAAATAATGATTCTGTTGATCCTAAT
GAAATTGAAATTGTTGAATCTAATATCGAAAATGTTGAGGAAGGAAAAAC
GAATTTATCCGAGGAATCAAATATAAACGAATCGTCAACAATTGATGAAC
CAGATCTTTCTGTCGATCCAAATAAAAGTGAACTTGCTGAGTTAAATATC
AATGGAGTTGATGAAGAAGAACCGAAACAACAACAACAAAATTTTAATTT
TGAAGAAGCATCAAAGGTTCATGAAAAAGATAATTCTGATGATCCTAATG
AAAATGAACTTGTTGAGCCTCATATCAAAAATATCGTAGAAGGTGAAAAG
AATATTTCCGATGAATCAAATGTAAAAGAATTGACAAAGGTTTTTGAAAA
ACAGCATTCTTTTGATCAAATTGAAGGGAATTCTGAGGAGAGAAATATAA
CTGATGATGTAAATTATAAAGATGTGTCACATGTTGATGAAACAGATCAT
GCTGATGATTCAAATGAAAGTGAACCTGCTGAGTCTAATTTCAATGAAGT
TGATGAGGAAGAAGGTAATTCTGAAGAGGAATCAAAAGTTA
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protein sequence of SMED30019664-orf-1

>SMED30019664-orf-1 ID=SMED30019664-orf-1|Name=SMED30019664-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=563bp
NSDEVIKVDVKDKSVNPDESELDEPNCIEIVEVDKNISEESNVKELLKVT
ETNHSVDQNEGELDEQIIIEIDKSEKNISDQVNVDELSKVDEIDHFADPN
ESESANSNINEVNKEETKQQELVNSKELSEVDEKDNSYNPNKKEFVEPNI
KNVDEVEKNIIEESYLKALSMIDGTKHSVDENEEELAELNIKNVNEKERS
LIYNANLIEVSKVDEIYYSEDPNENTLTEPITKEVDNIENIQTDYVKSEE
LKADETEYSVDQIERESAESNIDEVDEEEITQEKFNLKEVSKVDETDHSV
DPNESEPSGSNINEIGQGEKNISDKVNVDQVSKDDESDHSVDPNKSEPGK
SYINEIEGEITHKKINLDEVSKVDENNDSVDPNEIEIVESNIENVEEGKT
NLSEESNINESSTIDEPDLSVDPNKSELAELNINGVDEEEPKQQQQNFNF
EEASKVHEKDNSDDPNENELVEPHIKNIVEGEKNISDESNVKELTKVFEK
QHSFDQIEGNSEERNITDDVNYKDVSHVDETDHADDSNESEPAESNFNEV
DEEEGNSEEESKV
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0000418head
PLANA:0002089reproductive organ
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..30