SMED30019610

Overview
NameSMED30019610
Smed IDSMED30019610
Length (bp)1029
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30019610 (SMED30019610) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30019610 (SMED30019610) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30019610 (SMED30019610) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30019610 (SMED30019610) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30019610

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X2 cellSMED30019610 SmedASXL_065746SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
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BLAST of SMED30019610 vs. Planmine SMEST
Match: SMESG000034632.1 (SMESG000034632.1)

HSP 1 Score: 275.404 bits (703), Expect = 4.982e-91
Identity = 164/354 (46.33%), Postives = 206/354 (58.19%), Query Frame = 1 
Query:   28 EPRRTTWQSL---GINKRPMVENVQNDEPSPAQEIRDQACSLLTRLEDHQRQVDFHVVRARQYLPNVEDNVAAGRIADSYNYMPPSS--------------------------SKPIRNTKRPVFVYYEHPYTQPAIDE-NEKQHQHPQSR*QFKHGLQVWSNTNDVKFSFQ*MISCLPRILLYDSIL*RFV*EIK*FNEQLNNLRFADQG*CHCFAEMGSLETLQKELEHFRKLNKD*YTQLIQMAKQIQQTKASWMEPKKTKLLHQRLTAAQNGWADERQLNQSLRTQIRGLEVALSACQEWAAVTYPFVFAPANWPTERTATDPKATPKTNSYQPGRKERAKCRAARLLKN 999
            +PR + W +L     ++ P V   +    +P QE+ +QA  LL+RLE HQRQVDF VVRARQYL NVE+++AAGRIAD+ NY+PP S                          S   RNTKRPV+VY E PY QPA +E ++  H+ P             + TN++             I  Y S++                     QG    F +M SL+ LQ+ELEH   +NK+ Y Q+IQ++K+IQQTKA+W++ KK KLL+QRL AAQ GW +ERQLNQSLRTQI+GLEVALSACQE AAV YPFVFAPA      T  DPK TPK + Y+PGRKE AK RAARL  N
Sbjct:   10 QPRESRWNALRQEEDDELPFVAIKRECGRNPVQEMHEQARFLLSRLEYHQRQVDFQVVRARQYLANVEEDIAAGRIADTDNYIPPRSPTRNCVDERMKVSSTSSHIAIPPRSRSNRTRNTKRPVYVYRERPYAQPAAEECDDGYHRIP-------------TFTNNL-------------ITDYKSLI------------------IGRQGLISLFTDMESLKILQEELEHAHSINKNFYEQIIQLSKKIQQTKATWIDSKKAKLLYQRLRAAQKGWTEERQLNQSLRTQIKGLEVALSACQEGAAVKYPFVFAPAQLAYRGTTIDPKTTPKASGYRPGRKEWAKHRAARLFNN 319
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BLAST of SMED30019610 vs. Planmine SMEST
Match: SMESG000058567.1 (SMESG000058567.1)

HSP 1 Score: 188.348 bits (477), Expect = 1.672e-57
Identity = 94/124 (75.81%), Postives = 102/124 (82.26%), Query Frame = 1 
Query:   22 QPEPRRTTWQSLGINKRPMVENVQNDEPSPAQEIRDQACSLLTRLEDHQRQVDFHVVRARQYLPNVEDNVAAGRIADSYNYMPPSSSKPIRNTKRPVFVYYEHPYTQPAIDEN-EKQHQHPQSR 390
            Q EPRRTTWQSL  N+RP++ N +N   SPAQEIRD A SLLT LEDHQRQVDFHVVR  QYL NVED++ AGRIADSYNYMPP SSKPIRNTKRPVFVYY +PYTQP  DEN E QHQ+PQ R
Sbjct:  160 QSEPRRTTWQSLENNRRPLLSNGRNSGQSPAQEIRDHARSLLTHLEDHQRQVDFHVVRVIQYLANVEDDITAGRIADSYNYMPPPSSKPIRNTKRPVFVYYGYPYTQPVTDENVEGQHQNPQFR 283
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BLAST of SMED30019610 vs. Planmine SMEST
Match: SMESG000001289.1 (SMESG000001289.1)

HSP 1 Score: 179.489 bits (454), Expect = 8.139e-56
Identity = 89/140 (63.57%), Postives = 105/140 (75.00%), Query Frame = 1 
Query:  574 CHCFAEMGSLETLQKELEHFRKLNKD*YTQLIQMAKQIQQTKASWMEPKKTKLLHQRLTAAQNGWADERQLNQSLRTQIRGLEVALSACQEWAAVTYPFVFAPANWPTERTATDPKATPKTNSYQPGRKERAKCRAARLL 993
                 +M SL+  QKEL+H RK NK+ Y Q+IQ++ +IQ TK +WMEP+KTK L+Q LTAAQ GWADE+QLNQSLR QI+GLEVALSACQE AAVTY  VFAPA      T   PK TP T+SY+PGRKERAK RA R+L
Sbjct:   11 IFVVTQMESLKIPQKELDHARKTNKNFYEQIIQLSTEIQPTKTAWMEPRKTKALYQMLTAAQKGWADEKQLNQSLRMQIKGLEVALSACQEGAAVTYTLVFAPAQLAYRETVATPKTTPATSSYRPGRKERAKRRATRML 150
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BLAST of SMED30019610 vs. Planmine SMEST
Match: SMESG000020232.1 (SMESG000020232.1)

HSP 1 Score: 191.815 bits (486), Expect = 9.748e-56
Identity = 101/139 (72.66%), Postives = 108/139 (77.70%), Query Frame = 3 
Query:  555 IRGSRLMSLFCRDGITGNSAKGTRTFS*TQQGLIYTTYPDGKANSTNQSLMDGTEENKTASSKTYCSPKWMGGRKAVKSKPADTDKGTGGRIVSLPRMGSSNLPLCLCPCQLAY-REDGH*SESYPKDK*LSTWTKGAG 968
            IRGS+LMSLFCRDGITGNSAKGTRTFS TQQG +YTTYPDGK NSTNQSLMD         ++    PK MGGRKAVKSKPADTDKGT GRIV LP  GSSNL  CLCPC     R++GH S+ YPKDK LSTWTKGAG
Sbjct:  483 IRGSKLMSLFCRDGITGNSAKGTRTFSSTQQGFLYTTYPDGKGNSTNQSLMD--------ENRNLLQPKSMGGRKAVKSKPADTDKGTEGRIVGLPGRGSSNLLPCLCPCPTGLPRDNGHGSKGYPKDKQLSTWTKGAG 613
[Close]
BLAST of SMED30019610 vs. Planmine SMEST
Match: SMESG000078007.1 (SMESG000078007.1)

HSP 1 Score: 178.333 bits (451), Expect = 1.202e-55
Identity = 91/132 (68.94%), Postives = 103/132 (78.03%), Query Frame = 3 
Query:  573 MSLFCRDGITGNSAKGTRTFS*TQQGLIYTTYPDGKANSTNQSLMDGTEENKTASSKTYCSPKWMGGRKAVKSKPADTDKGTGGRIVSLPRMGSSNLPLCLCPCQLAYREDGH*SESYPKDK*LSTWTKGAG 968
            MSLFCRDGITGNSAKGT+T + TQ+G +YTTYPD K N TNQSLMDGTEE+KTA SK Y SP+ MGGRKAVKSKP + DKGTGGRI+ LP  GSSN+P CLCPC      DG   E+Y  +K LST T+GAG
Sbjct:    1 MSLFCRDGITGNSAKGTKTRAQTQRGFLYTTYPDVKGNPTNQSLMDGTEEDKTALSKAYRSPEGMGGRKAVKSKPQNADKGTGGRIIGLPGRGSSNIPPCLCPCPAGLPRDGSDPENYSSNKQLSTRTEGAG 132
The following BLAST results are available for this feature:
BLAST of SMED30019610 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019610 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019610 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019610 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019610 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019610 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019610 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019610 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019610 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019610 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019610 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019610 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019610 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019610 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000034632.14.982e-9146.33SMESG000034632.1[more]
SMESG000058567.11.672e-5775.81SMESG000058567.1[more]
SMESG000001289.18.139e-5663.57SMESG000001289.1[more]
SMESG000020232.19.748e-5672.66SMESG000020232.1[more]
SMESG000078007.11.202e-5568.94SMESG000078007.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30019610 ID=SMED30019610|Name=SMED30019610|organism=Schmidtea mediterranea sexual|type=transcript|length=1029bp
CTTCTATCGACAGCTCGTGAACAGCCGGAGCCCCGACGGACCACCTGGCA
ATCGTTGGGAATTAACAAAAGACCGATGGTGGAGAATGTACAAAATGATG
AGCCTAGTCCGGCCCAAGAAATTCGGGATCAAGCCTGCTCTCTCCTTACA
CGCCTCGAGGACCACCAACGGCAGGTCGACTTTCACGTGGTCAGGGCCAG
ACAGTATCTGCCGAACGTAGAAGACAATGTCGCGGCCGGTAGGATTGCGG
ATTCCTACAATTATATGCCACCGTCCAGCTCCAAACCGATCCGAAACACA
AAGCGACCGGTCTTTGTGTATTATGAGCACCCATACACTCAGCCGGCCAT
AGACGAAAATGAAAAGCAGCATCAACACCCCCAATCCCGTTAGCAATTCA
AACACGGACTCCAAGTTTGGAGTAACACTAATGATGTTAAATTTAGTTTC
CAATAAATGATTAGTTGTCTTCCACGAATTTTGTTGTATGATTCGATATT
GTAAAGATTTGTATGAGAAATTAAATAATTTAATGAGCAATTGAATAATT
TAAGATTCGCGGATCAAGGTTAATGTCATTGTTTTGCAGAGATGGGATCA
CTGGAAACTCTGCAAAAGGAACTAGAACATTTTCGTAAACTCAACAAGGA
TTAATATACACAACTTATCCAGATGGCAAAGCAAATTCAACAAACCAAAG
CCTCATGGATGGAACCGAAGAAAACAAAACTGCTTCATCAAAGACTTACT
GCAGCCCAAAATGGATGGGCGGACGAAAGGCAGTTAAATCAAAGCCTGCG
GACACAGATAAGGGGACTGGAGGTCGCATTGTCAGCCTGCCAAGAATGGG
CAGCAGTAACTTACCCCTTTGTCTTTGCCCCTGCCAACTGGCCTACCGAG
AGGACGGCCACTGATCCGAAAGCTACCCCAAAGACAAATAGCTATCAACC
TGGACGAAAGGAGCGGGCTAAATGTCGAGCCGCTCGATTATTAAAAAATG
CATAATTAACTTATACAATGTGTTCATTT
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protein sequence of SMED30019610-orf-1

>SMED30019610-orf-1 ID=SMED30019610-orf-1|Name=SMED30019610-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=111bp
MAKQIQQTKASWMEPKKTKLLHQRLTAAQNGWADERQLNQSLRTQIRGLE
VALSACQEWAAVTYPFVFAPANWPTERTATDPKATPKTNSYQPGRKERAK
CRAARLLKNA*
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protein sequence of SMED30019610-orf-2

>SMED30019610-orf-2 ID=SMED30019610-orf-2|Name=SMED30019610-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=131bp
LLSTAREQPEPRRTTWQSLGINKRPMVENVQNDEPSPAQEIRDQACSLLT
RLEDHQRQVDFHVVRARQYLPNVEDNVAAGRIADSYNYMPPSSSKPIRNT
KRPVFVYYEHPYTQPAIDENEKQHQHPQSR*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002111X2 cell
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableTMHMMTMhelixcoord: 10..29