Pre-mRNA-splicing factor ISY1

Overview
NamePre-mRNA-splicing factor ISY1
Smed IDSMED30019578
Length (bp)999
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Pre-mRNA-splicing factor ISY1 (SMED30019578) t-SNE clustered cells

Violin plots show distribution of expression levels for Pre-mRNA-splicing factor ISY1 (SMED30019578) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Pre-mRNA-splicing factor ISY1 (SMED30019578) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Pre-mRNA-splicing factor ISY1 (SMED30019578) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30019578

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30019578 SmedASXL_011498SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30019578 SmedASXL_011498SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
neoblastSMED30019578 dd_Smed_v4_3703_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Pre-mRNA-splicing factor ISY1 vs. Ensembl Human
Match: ISY1 (ISY1 splicing factor homolog [Source:HGNC Symbol;Acc:HGNC:29201])

HSP 1 Score: 207.223 bits (526), Expect = 1.633e-64
Identity = 133/294 (45.24%), Postives = 183/294 (62.24%), Query Frame = 1 
Query:   49 MARSAEKKMTALARWRAVY--EDKISFRERPKLASECNSLRDANMFRRQILNEVTRKITQIQNSSLGEFKLRDLNDEINRLFREKSHWEDRIVELGGTDHKRNSFLLMEKEGKALPGNWGYRYFGACKDLPGVRELFQNESDGVSKKVRVDL----NNQYYGYMDEADGVILSIEETAEKEFREKLFQKSKKADKSHEEIEPNIYETGDNPADIERDAIYEDLRKSITPDXXXXXXPELVTDLESRVKRFTNIIVPSQQEIEXXXXXXXXXXXXXNYMSDELIADMVNSGTLLG 912
            MAR+AEK MTALAR+R     E K+  R RP LASEC  L  A  +RRQI+ E+++K+ QIQN+ LGEF++RDLNDEIN+L REK HWE RI ELGG D+ +    +++ EGK +PGN GY+YFGA KDLPGVRELF+ E     +K R +L    + +YYGY+DE DGVI+ +E+  EK+ R +L +K K A++          E  +   +I    IY     ++T +     G +     +S+ K   ++ VPSQQEIE+ +V  KK +LL+ Y S+ L A    +  LLG
Sbjct:    1 MARNAEKAMTALARFRQAQLEEGKVKER-RPFLASECTELPKAEKWRRQIIGEISKKVAQIQNAGLGEFRIRDLNDEINKLLREKGHWEVRIKELGGPDYGKVGPKMLDHEGKEVPGNRGYKYFGAAKDLPGVRELFEKEPLPPPRKTRAELMKAIDFEYYGYLDEDDGVIVPLEQEYEKKLRAELVEKWK-AEREARLARGEKEEEEEEEEEIN---IY-----AVTEEESDEEGSQEKGGDDSQQKFIAHVPVPSQQEIEEALVRRKKMELLQKYASETLQAQSEEARRLLG 284
[Close]
BLAST of Pre-mRNA-splicing factor ISY1 vs. Ensembl Human
Match: ISY1-RAB43 (ISY1-RAB43 readthrough [Source:HGNC Symbol;Acc:HGNC:42969])

HSP 1 Score: 207.608 bits (527), Expect = 3.408e-64
Identity = 136/305 (44.59%), Postives = 187/305 (61.31%), Query Frame = 1 
Query:   49 MARSAEKKMTALARWRAVY--EDKISFRERPKLASECNSLRDANMFRRQILNEVTRKITQIQNSSLGEFKLRDLNDEINRLFREKSHWEDRIVELGGTDHKRNSFLLMEKEGKALPGNWGYRYFGACKDLPGVRELFQNESDGVSKKVRVDL----NNQYYGYMDEADGVILSIEETAEKEFREKLFQKSKKADKSHEEIEPNIYETGDNPADIERDAIYEDLRKSITPDXXXXXXPELVTDLESRVKRFTNIIVPSQQEIEXXXXXXXXXXXXXNYMSDELIADMVNSGTLLGMDT---PAADG 936
            MAR+AEK MTALAR+R     E K+  R RP LASEC  L  A  +RRQI+ E+++K+ QIQN+ LGEF++RDLNDEIN+L REK HWE RI ELGG D+ +    +++ EGK +PGN GY+YFGA KDLPGVRELF+ E     +K R +L    + +YYGY+DE DGVI+ +E+  EK+ R +L +K K A++          E  +   +I    IY     ++T +     G +     +S+ K   ++ VPSQQEIE+ +V  KK +LL+ Y S+ L A    +  LLG  +   PA  G
Sbjct:    1 MARNAEKAMTALARFRQAQLEEGKVKER-RPFLASECTELPKAEKWRRQIIGEISKKVAQIQNAGLGEFRIRDLNDEINKLLREKGHWEVRIKELGGPDYGKVGPKMLDHEGKEVPGNRGYKYFGAAKDLPGVRELFEKEPLPPPRKTRAELMKAIDFEYYGYLDEDDGVIVPLEQEYEKKLRAELVEKWK-AEREARLARGEKEEEEEEEEEIN---IY-----AVTEEESDEEGSQEKGGDDSQQKFIAHVPVPSQQEIEEALVRRKKMELLQKYASETLQAQSEEARRLLGCRSGTRPARSG 295
[Close]
BLAST of Pre-mRNA-splicing factor ISY1 vs. Ensembl Human
Match: ISY1 (ISY1 splicing factor homolog [Source:HGNC Symbol;Acc:HGNC:29201])

HSP 1 Score: 196.823 bits (499), Expect = 5.447e-61
Identity = 100/180 (55.56%), Postives = 129/180 (71.67%), Query Frame = 1 
Query:   49 MARSAEKKMTALARWRAVY--EDKISFRERPKLASECNSLRDANMFRRQILNEVTRKITQIQNSSLGEFKLRDLNDEINRLFREKSHWEDRIVELGGTDHKRNSFLLMEKEGKALPGNWGYRYFGACKDLPGVRELFQNESDGVSKKVRVDL----NNQYYGYMDEADGVILSIEETAEK 570
            MAR+AEK MTALAR+R     E K+  R RP LASEC  L  A  +RRQI+ E+++K+ QIQN+ LGEF++RDLNDEIN+L REK HWE RI ELGG D+ +    +++ EGK +PGN GY+YFGA KDLPGVRELF+ E     +K R +L    + +YYGY+DE DGVI+ +E+  EK
Sbjct:    1 MARNAEKAMTALARFRQAQLEEGKVKER-RPFLASECTELPKAEKWRRQIIGEISKKVAQIQNAGLGEFRIRDLNDEINKLLREKGHWEVRIKELGGPDYGKVGPKMLDHEGKEVPGNRGYKYFGAAKDLPGVRELFEKEPLPPPRKTRAELMKAIDFEYYGYLDEDDGVIVPLEQEYEK 179
[Close]
BLAST of Pre-mRNA-splicing factor ISY1 vs. Ensembl Human
Match: ISY1 (ISY1 splicing factor homolog [Source:HGNC Symbol;Acc:HGNC:29201])

HSP 1 Score: 195.667 bits (496), Expect = 8.971e-60
Identity = 133/315 (42.22%), Postives = 185/315 (58.73%), Query Frame = 1 
Query:   49 MARSAEKKMTALARWRAVYEDKISFRER-PKLASECNSLRDANMFRRQILNEVTRKITQIQNSSLGEFKLRDLNDEINRLFREKSHWEDRIVELGGTDHKRNSFLLMEKEGKALPGNWGYRYFGACKDLPGVRELFQNESD----------------GVS------KKVRVDL----NNQYYGYMDEADGVILSIEETAEKEFREKLFQKSKKADKSHEEIEPNIYETGDNPADIERDAIYEDLRKSITPDXXXXXXPELVTDLESRVKRFTNIIVPSQQEIEXXXXXXXXXXXXXNYMSDELIADMVNSGTLLG 912
            MAR+AEK MTALAR+R    ++   +ER P LASEC  L  A  +RRQI+ E+++K+ QIQN+ LGEF++RDLNDEIN+L REK HWE RI ELGG D+ +    +++ EGK +PGN GY+YFGA KDLPGVRELF+ E                  G S      +K R +L    + +YYGY+DE DGVI+ +E+  EK+ R +L +K K A++          E  +   +I    IY     ++T +     G +     +S+ K   ++ VPSQQEIE+ +V  KK +LL+ Y S+ L A    +  LLG
Sbjct:    1 MARNAEKAMTALARFRQAQLEEGKVKERRPFLASECTELPKAEKWRRQIIGEISKKVAQIQNAGLGEFRIRDLNDEINKLLREKGHWEVRIKELGGPDYGKVGPKMLDHEGKEVPGNRGYKYFGAAKDLPGVRELFEKERQVRWLMPVIPALWEAEAGGSQALPPPRKTRAELMKAIDFEYYGYLDEDDGVIVPLEQEYEKKLRAELVEKWK-AEREARLARGEKEEEEEEEEEIN---IY-----AVTEEESDEEGSQEKGGDDSQQKFIAHVPVPSQQEIEEALVRRKKMELLQKYASETLQAQSEEARRLLG 306
[Close]
BLAST of Pre-mRNA-splicing factor ISY1 vs. Ensembl Human
Match: ISY1 (ISY1 splicing factor homolog [Source:HGNC Symbol;Acc:HGNC:29201])

HSP 1 Score: 135.576 bits (340), Expect = 1.463e-38
Identity = 65/119 (54.62%), Postives = 86/119 (72.27%), Query Frame = 1 
Query:  232 SSLGEFKLRDLNDEINRLFREKSHWEDRIVELGGTDHKRNSFLLMEKEGKALPGNWGYRYFGACKDLPGVRELFQNESDGVSKKVRVDL----NNQYYGYMDEADGVILSIEETAEKEF 576
            + LGEF++RDLNDEIN+L REK HWE RI ELGG D+ +    +++ EGK +PGN GY+YFGA KDLPGVRELF+ E     +K R +L    + +YYGY+DE DGVI+ +E+  EK+ 
Sbjct:    1 AGLGEFRIRDLNDEINKLLREKGHWEVRIKELGGPDYGKVGPKMLDHEGKEVPGNRGYKYFGAAKDLPGVRELFEKEPLPPPRKTRAELMKAIDFEYYGYLDEDDGVIVPLEQEYEKKL 119
[Close]
BLAST of Pre-mRNA-splicing factor ISY1 vs. Ensembl Celegans
Match: isy-1 (ISY splicing factor homolog [Source:UniProtKB/TrEMBL;Acc:Q20716])

HSP 1 Score: 174.481 bits (441), Expect = 1.581e-52
Identity = 98/216 (45.37%), Postives = 136/216 (62.96%), Query Frame = 1 
Query:   49 MARSAEKKMTALARWRAVYED--KISFRERPKLASECNSLRDANMFRRQILNEVTRKITQIQNSSLGEFKLRDLNDEINRLFREKSHWEDRIVELGGTDHKRNSFLLMEKEGKALPGNWGYRYFGACKDLPGVRELFQNESDG-VSKKVRVDL----NNQYYGYMDEADGVILSIEETAE--------KEFREKLFQKSKKADKSHEEIEPNIYET 651
            MAR+AEK MTALARWR + E+  +     RP    +C +L DA  FRR+I+ + ++KIT IQN  LGEFKLRDLNDE+NRL + K  WE RI ELGGTD+++ +   ++  G+    + GY+YFGA KDLPGVRELF+  ++G   ++ R DL    +  Y+GY+D+ DG ++ +E+  E        KEF EK  QK + A  +  E   NIY+ 
Sbjct:    1 MARNAEKAMTALARWRRMKEEEERGPIARRPHDVKDCRNLSDAERFRREIVRDASKKITAIQNPGLGEFKLRDLNDEVNRLIKLKHAWEQRIRELGGTDYRKYAQKELDAIGRETGNSRGYKYFGAAKDLPGVRELFEKSTEGEEQRRHRADLLRNIDAHYFGYLDDEDGRLIPLEKLIEEKNIERINKEFAEKQAQKQQTASDAAPE---NIYKV 213
[Close]
BLAST of Pre-mRNA-splicing factor ISY1 vs. Ensembl Celegans
Match: isy-1 (ISY splicing factor homolog [Source:UniProtKB/TrEMBL;Acc:Q20716])

HSP 1 Score: 174.481 bits (441), Expect = 1.581e-52
Identity = 98/216 (45.37%), Postives = 136/216 (62.96%), Query Frame = 1 
Query:   49 MARSAEKKMTALARWRAVYED--KISFRERPKLASECNSLRDANMFRRQILNEVTRKITQIQNSSLGEFKLRDLNDEINRLFREKSHWEDRIVELGGTDHKRNSFLLMEKEGKALPGNWGYRYFGACKDLPGVRELFQNESDG-VSKKVRVDL----NNQYYGYMDEADGVILSIEETAE--------KEFREKLFQKSKKADKSHEEIEPNIYET 651
            MAR+AEK MTALARWR + E+  +     RP    +C +L DA  FRR+I+ + ++KIT IQN  LGEFKLRDLNDE+NRL + K  WE RI ELGGTD+++ +   ++  G+    + GY+YFGA KDLPGVRELF+  ++G   ++ R DL    +  Y+GY+D+ DG ++ +E+  E        KEF EK  QK + A  +  E   NIY+ 
Sbjct:    1 MARNAEKAMTALARWRRMKEEEERGPIARRPHDVKDCRNLSDAERFRREIVRDASKKITAIQNPGLGEFKLRDLNDEVNRLIKLKHAWEQRIRELGGTDYRKYAQKELDAIGRETGNSRGYKYFGAAKDLPGVRELFEKSTEGEEQRRHRADLLRNIDAHYFGYLDDEDGRLIPLEKLIEEKNIERINKEFAEKQAQKQQTASDAAPE---NIYKV 213
[Close]
BLAST of Pre-mRNA-splicing factor ISY1 vs. Ensembl Fly
Match: CG9667 (gene:FBgn0037550 transcript:FBtr0081815)

HSP 1 Score: 191.815 bits (486), Expect = 4.067e-59
Identity = 93/187 (49.73%), Postives = 129/187 (68.98%), Query Frame = 1 
Query:   49 MARSAEKKMTALARWRAVYEDKISFRER-PKLASECNSLRDANMFRRQILNEVTRKITQIQNSSLGEFKLRDLNDEINRLFREKSHWEDRIVELGGTDHKRNSFLLMEKEGKALPGNWGYRYFGACKDLPGVRELFQNESDGVSKKVRV----DLNNQYYGYMDEADGVILSIEETAEKEFREKLFQ 594
            MAR+AEK MT LARWRA  E +   +ER P LASEC+ L     FR +I+ ++++K+ QIQN+ LGEF++RDLNDEIN+L REK HWE++I  LGG  ++R    + + EG+ +PGN GY+YFGA KDLPGVRELF+ +     +K R     D++ +YYGY D+ DGV++ +EE  E+   +K  Q
Sbjct:    1 MARNAEKAMTTLARWRAAKEVESGEKERRPYLASECHDLPRCEKFRLEIIRDISKKVAQIQNAGLGEFRIRDLNDEINKLLREKRHWENQISSLGGPHYRRYGPKMFDAEGREVPGNRGYKYFGAAKDLPGVRELFEQDPPPPPRKSRAELMKDIDAEYYGYRDDEDGVLIPLEERIERAAIQKAVQ 187
[Close]
BLAST of Pre-mRNA-splicing factor ISY1 vs. Ensembl Fly
Match: CG9667 (gene:FBgn0037550 transcript:FBtr0339725)

HSP 1 Score: 181.03 bits (458), Expect = 5.974e-55
Identity = 87/179 (48.60%), Postives = 122/179 (68.16%), Query Frame = 1 
Query:   73 MTALARWRAVYEDKISFRER-PKLASECNSLRDANMFRRQILNEVTRKITQIQNSSLGEFKLRDLNDEINRLFREKSHWEDRIVELGGTDHKRNSFLLMEKEGKALPGNWGYRYFGACKDLPGVRELFQNESDGVSKKVRV----DLNNQYYGYMDEADGVILSIEETAEKEFREKLFQ 594
            MT LARWRA  E +   +ER P LASEC+ L     FR +I+ ++++K+ QIQN+ LGEF++RDLNDEIN+L REK HWE++I  LGG  ++R    + + EG+ +PGN GY+YFGA KDLPGVRELF+ +     +K R     D++ +YYGY D+ DGV++ +EE  E+   +K  Q
Sbjct:    1 MTTLARWRAAKEVESGEKERRPYLASECHDLPRCEKFRLEIIRDISKKVAQIQNAGLGEFRIRDLNDEINKLLREKRHWENQISSLGGPHYRRYGPKMFDAEGREVPGNRGYKYFGAAKDLPGVRELFEQDPPPPPRKSRAELMKDIDAEYYGYRDDEDGVLIPLEERIERAAIQKAVQ 179
[Close]
BLAST of Pre-mRNA-splicing factor ISY1 vs. Ensembl Zebrafish
Match: isy1 (ISY1 splicing factor homolog [Source:ZFIN;Acc:ZDB-GENE-030131-5864])

HSP 1 Score: 213.772 bits (543), Expect = 2.116e-67
Identity = 133/295 (45.08%), Postives = 186/295 (63.05%), Query Frame = 1 
Query:   49 MARSAEKKMTALARWRAVY--EDKISFRERPKLASECNSLRDANMFRRQILNEVTRKITQIQNSSLGEFKLRDLNDEINRLFREKSHWEDRIVELGGTDHKRNSFLLMEKEGKALPGNWGYRYFGACKDLPGVRELFQNESDGVSKKVRV----DLNNQYYGYMDEADGVILSIEETAEKEFREKLFQKSKKADKSHEEIEPNIYETGDNPADIERDAIYEDLRKSITPDXXXXXXPELVTDLESRVKRFTNIIVPSQQEIEXXXXXXXXXXXXXNYMSDELIADMVNSGTLLGM 915
            MAR+AEK MTALAR+R     E K+  R RP LASECN L  A  +RRQI++E+++K+ QIQN+ LGEFK+RDLNDEIN+L REK HWE RI ELGG D++R    +++ EGK +PGN GY+YFGA KDLPGVRELF++E     +K R     D++  YYGY DE DGV+L +E+  EK+   +  +K K   ++   +   + +T +     E + IY  +R     D   G   E     +S +   +++ VPSQ+EIE+ +V  KK +LL+ Y S+ L+A    +  LLG+
Sbjct:    1 MARNAEKAMTALARFRQAQLEEGKVKER-RPYLASECNELPKAEKWRRQIISEISKKVAQIQNAGLGEFKIRDLNDEINKLLREKGHWEVRIKELGGPDYRRFGPKMLDHEGKEVPGNRGYKYFGAAKDLPGVRELFESEPVPPPRKTRAELMKDIDADYYGYRDEDDGVLLPLEQEYEKQVMAEAVEKWKADKEARLAVGGAVKDTEE-----EEEYIYA-VRDEERSDDETGDQMEGEDGAQSFI---SHVPVPSQKEIEEALVRRKKMELLQKYASESLMAQSQEAKALLGL 285
[Close]
BLAST of Pre-mRNA-splicing factor ISY1 vs. Ensembl Xenopus
Match: ENSXETT00000024318.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:KV463450.1:306:1712:-1 gene:ENSXETG00000015381.1 transcript:ENSXETT00000024318.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 102.834 bits (255), Expect = 1.501e-26
Identity = 46/71 (64.79%), Postives = 57/71 (80.28%), Query Frame = 1 
Query:  130 RPKLASECNSLRDANMFRRQILNEVTRKITQIQNSSLGEFKLRDLNDEINRLFREKSHWEDRIVELGGTDH 342
            RP LASECN L  A  +RRQI+ E+++K+ QIQN+ LGEF++RDLNDEIN+L REK HWE RI ELGG D+
Sbjct:   25 RPFLASECNELPKAEKWRRQIIGEISKKVAQIQNAGLGEFRIRDLNDEINKLIREKGHWEVRIKELGGPDY 95
[Close]
BLAST of Pre-mRNA-splicing factor ISY1 vs. Ensembl Mouse
Match: Isy1 (ISY1 splicing factor homolog [Source:MGI Symbol;Acc:MGI:1923310])

HSP 1 Score: 206.068 bits (523), Expect = 2.438e-64
Identity = 132/294 (44.90%), Postives = 182/294 (61.90%), Query Frame = 1 
Query:   49 MARSAEKKMTALARWRAVY--EDKISFRERPKLASECNSLRDANMFRRQILNEVTRKITQIQNSSLGEFKLRDLNDEINRLFREKSHWEDRIVELGGTDHKRNSFLLMEKEGKALPGNWGYRYFGACKDLPGVRELFQNESDGVSKKVRVDL----NNQYYGYMDEADGVILSIEETAEKEFREKLFQKSKKADKSHEEIEPNIYETGDNPADIERDAIYEDLRKSITPDXXXXXXPELVTDLESRVKRFTNIIVPSQQEIEXXXXXXXXXXXXXNYMSDELIADMVNSGTLLG 912
            MAR+AEK MTALAR+R     E K+  R RP LASEC  L  A  +RRQI+ E+++K+ QIQN+ LGEF++RDLNDEIN+L REK HWE RI ELGG D+ +    +++ EGK +PGN GY+YFGA KDLPGVRELF+ E     +K R +L    + +YYGY+DE DGVI+ +E+  EK+ R +L +K  KA++          E  +   +I    IY     ++T +     G +     + + K   ++ VPSQQEIE+ +V  KK +LL+ Y S+ L A    +  LLG
Sbjct:    1 MARNAEKAMTALARFRQAQLEEGKVKER-RPFLASECTELPKAEKWRRQIIGEISKKVAQIQNAGLGEFRIRDLNDEINKLLREKGHWEVRIKELGGPDYGKVGPKMLDHEGKEVPGNRGYKYFGAAKDLPGVRELFEKEPLPPPRKTRAELMKAIDFEYYGYLDEDDGVIVPLEQEYEKKLRAELVEKW-KAEREARLARGEKEEEEEEEEEIN---IY-----AVTEEESDEEGNQEKAGEDGQQKFIAHVPVPSQQEIEEALVRRKKMELLQKYASETLQAQSEEAKRLLG 284
[Close]
BLAST of Pre-mRNA-splicing factor ISY1 vs. Ensembl Mouse
Match: Gm45140 (predicted gene 45140 [Source:MGI Symbol;Acc:MGI:5753716])

HSP 1 Score: 191.43 bits (485), Expect = 1.582e-59
Identity = 96/182 (52.75%), Postives = 129/182 (70.88%), Query Frame = 1 
Query:   73 MTALARWRAVYEDKISFRER-PKLASECNSLRDANMFRRQILNEVTRKITQIQNSSLGEFKLRDLNDEINRLFREKSHWEDRIVELGGTDHKRNSFLLMEKEGKALPGNWGYRYFGACKDLPGVRELFQNESDGVSKKVRVDL----NNQYYGYMDEADGVILSIEETAEKEFREKLFQKSK 603
            MTALAR+R    ++   +ER P LASEC  L  A  +RRQI+ E+++K+ QIQN+ LGEF++RDLNDEIN+L REK HWE RI ELGG D+ +    +++ EGK +PGN GY+YFGA KDLPGVRELF+ E     +K R +L    + +YYGY+DE DGVI+ +E+  EK+ R +L +K K
Sbjct:    1 MTALARFRQAQLEEGKVKERRPFLASECTELPKAEKWRRQIIGEISKKVAQIQNAGLGEFRIRDLNDEINKLLREKGHWEVRIKELGGPDYGKVGPKMLDHEGKEVPGNRGYKYFGAAKDLPGVRELFEKEPLPPPRKTRAELMKAIDFEYYGYLDEDDGVIVPLEQEYEKKLRAELVEKWK 182
[Close]
BLAST of Pre-mRNA-splicing factor ISY1 vs. Ensembl Mouse
Match: Isy1 (ISY1 splicing factor homolog [Source:MGI Symbol;Acc:MGI:1923310])

HSP 1 Score: 120.168 bits (300), Expect = 6.883e-33
Identity = 59/99 (59.60%), Postives = 75/99 (75.76%), Query Frame = 1 
Query:   49 MARSAEKKMTALARWRAVYEDKISFRER-PKLASECNSLRDANMFRRQILNEVTRKITQIQNSSLGEFKLRDLNDEINRLFREKSHWEDRIVELGGTDH 342
            MAR+AEK MTALAR+R    ++   +ER P LASEC  L  A  +RRQI+ E+++K+ QIQN+ LGEF++RDLNDEIN+L REK HWE RI ELGG D+
Sbjct:    1 MARNAEKAMTALARFRQAQLEEGKVKERRPFLASECTELPKAEKWRRQIIGEISKKVAQIQNAGLGEFRIRDLNDEINKLLREKGHWEVRIKELGGPDY 99
[Close]
BLAST of Pre-mRNA-splicing factor ISY1 vs. Ensembl Mouse
Match: Isy1 (ISY1 splicing factor homolog [Source:MGI Symbol;Acc:MGI:1923310])

HSP 1 Score: 81.6481 bits (200), Expect = 5.178e-19
Identity = 46/95 (48.42%), Postives = 61/95 (64.21%), Query Frame = 1 
Query:   49 MARSAEKKMTALARWRAVYEDKISFRER-PKLASECNSLRDANMFRRQI--------------LNEVTRKITQIQNSSLGEFKLRDLNDEINRLF 288
            MAR+AEK MTALAR+R    ++   +ER P LASEC  L  A  +RRQI              + E+++K+ QIQN+ LGEF++RDLNDEIN+L 
Sbjct:    1 MARNAEKAMTALARFRQAQLEEGKVKERRPFLASECTELPKAEKWRRQICSKMMRCACCFPQIIGEISKKVAQIQNAGLGEFRIRDLNDEINKLL 95
[Close]
BLAST of Pre-mRNA-splicing factor ISY1 vs. Ensembl Mouse
Match: Isy1 (ISY1 splicing factor homolog [Source:MGI Symbol;Acc:MGI:1923310])

HSP 1 Score: 43.8986 bits (102), Expect = 5.643e-6
Identity = 28/50 (56.00%), Postives = 33/50 (66.00%), Query Frame = 1 
Query:   49 MARSAEKKMTALARWRAVY--EDKISFRERPKLASECNSLRDANMFRRQI 192
            MAR+AEK MTALAR+R     E K+  R RP LASEC  L  A  +RRQ+
Sbjct:    1 MARNAEKAMTALARFRQAQLEEGKVKER-RPFLASECTELPKAEKWRRQL 49
[Close]
BLAST of Pre-mRNA-splicing factor ISY1 vs. UniProt/SwissProt
Match: sp|Q9ULR0|ISY1_HUMAN (Pre-mRNA-splicing factor ISY1 homolog OS=Homo sapiens OX=9606 GN=ISY1 PE=1 SV=3)

HSP 1 Score: 207.223 bits (526), Expect = 7.842e-64
Identity = 133/294 (45.24%), Postives = 183/294 (62.24%), Query Frame = 1 
Query:   49 MARSAEKKMTALARWRAVY--EDKISFRERPKLASECNSLRDANMFRRQILNEVTRKITQIQNSSLGEFKLRDLNDEINRLFREKSHWEDRIVELGGTDHKRNSFLLMEKEGKALPGNWGYRYFGACKDLPGVRELFQNESDGVSKKVRVDL----NNQYYGYMDEADGVILSIEETAEKEFREKLFQKSKKADKSHEEIEPNIYETGDNPADIERDAIYEDLRKSITPDXXXXXXPELVTDLESRVKRFTNIIVPSQQEIEXXXXXXXXXXXXXNYMSDELIADMVNSGTLLG 912
            MAR+AEK MTALAR+R     E K+  R RP LASEC  L  A  +RRQI+ E+++K+ QIQN+ LGEF++RDLNDEIN+L REK HWE RI ELGG D+ +    +++ EGK +PGN GY+YFGA KDLPGVRELF+ E     +K R +L    + +YYGY+DE DGVI+ +E+  EK+ R +L +K K A++          E  +   +I    IY     ++T +     G +     +S+ K   ++ VPSQQEIE+ +V  KK +LL+ Y S+ L A    +  LLG
Sbjct:    1 MARNAEKAMTALARFRQAQLEEGKVKER-RPFLASECTELPKAEKWRRQIIGEISKKVAQIQNAGLGEFRIRDLNDEINKLLREKGHWEVRIKELGGPDYGKVGPKMLDHEGKEVPGNRGYKYFGAAKDLPGVRELFEKEPLPPPRKTRAELMKAIDFEYYGYLDEDDGVIVPLEQEYEKKLRAELVEKWK-AEREARLARGEKEEEEEEEEEIN---IY-----AVTEEESDEEGSQEKGGDDSQQKFIAHVPVPSQQEIEEALVRRKKMELLQKYASETLQAQSEEARRLLG 284
[Close]
BLAST of Pre-mRNA-splicing factor ISY1 vs. UniProt/SwissProt
Match: sp|Q6AYB3|ISY1_RAT (Pre-mRNA-splicing factor ISY1 homolog OS=Rattus norvegicus OX=10116 GN=Isy1 PE=2 SV=1)

HSP 1 Score: 206.068 bits (523), Expect = 1.620e-63
Identity = 135/306 (44.12%), Postives = 181/306 (59.15%), Query Frame = 1 
Query:   49 MARSAEKKMTALARWRAVY--EDKISFRERPKLASECNSLRDANMFRRQILNEVTRKITQIQNSSLGEFKLRDLNDEINRLFREKSHWEDRIVELGGTDHKRNSFLLMEKEGKALPGNWGYRYFGACKDLPGVRELFQNESDGVSKKVRVDL----NNQYYGYMDEADGVILSIEETAEKEFREKLFQKSKKADKSH----------EEIEPNIYETGDNPADIE--RDAIYEDLRKSITPDXXXXXXPELVTDLESRVKRFTNIIVPSQQEIEXXXXXXXXXXXXXNYMSDELIADMVNSGTLLG 912
            MAR+AEK MTALAR+R     E K+  R RP LASEC  L  A  +RRQI+ E+++K+ QIQN+ LGEF++RDLNDEIN+L REK HWE RI ELGG D+ +    +++ EGK +PGN GY+YFGA KDLPGVRELF+ E     +K R +L    + +YYGY+DE DGVI+ +E+  EK  R +L +K K   ++           EE E NIY   +  +D E  ++   ED                       + K   ++ VPSQQEIE+ +V  KK +LL+ Y S+ L A    +  LLG
Sbjct:    1 MARNAEKAMTALARFRQAQLEEGKVKER-RPFLASECTELPKAEKWRRQIIGEISKKVAQIQNAGLGEFRIRDLNDEINKLLREKGHWEVRIKELGGPDYGKVGPKMLDHEGKEVPGNRGYKYFGAAKDLPGVRELFEKEPLPPPRKTRAELMKAIDFEYYGYLDEDDGVIVPLEQEYEKRLRAELVEKWKAEREARLARGEKEEEEEEEEINIYAVTEEESDEEGSQEKAGED----------------------GQQKFIAHVPVPSQQEIEEALVRRKKMELLQKYASETLQAQSEEAKRLLG 283
[Close]
BLAST of Pre-mRNA-splicing factor ISY1 vs. UniProt/SwissProt
Match: sp|Q69ZQ2|ISY1_MOUSE (Pre-mRNA-splicing factor ISY1 homolog OS=Mus musculus OX=10090 GN=Isy1 PE=1 SV=2)

HSP 1 Score: 206.068 bits (523), Expect = 1.705e-63
Identity = 132/294 (44.90%), Postives = 182/294 (61.90%), Query Frame = 1 
Query:   49 MARSAEKKMTALARWRAVY--EDKISFRERPKLASECNSLRDANMFRRQILNEVTRKITQIQNSSLGEFKLRDLNDEINRLFREKSHWEDRIVELGGTDHKRNSFLLMEKEGKALPGNWGYRYFGACKDLPGVRELFQNESDGVSKKVRVDL----NNQYYGYMDEADGVILSIEETAEKEFREKLFQKSKKADKSHEEIEPNIYETGDNPADIERDAIYEDLRKSITPDXXXXXXPELVTDLESRVKRFTNIIVPSQQEIEXXXXXXXXXXXXXNYMSDELIADMVNSGTLLG 912
            MAR+AEK MTALAR+R     E K+  R RP LASEC  L  A  +RRQI+ E+++K+ QIQN+ LGEF++RDLNDEIN+L REK HWE RI ELGG D+ +    +++ EGK +PGN GY+YFGA KDLPGVRELF+ E     +K R +L    + +YYGY+DE DGVI+ +E+  EK+ R +L +K  KA++          E  +   +I    IY     ++T +     G +     + + K   ++ VPSQQEIE+ +V  KK +LL+ Y S+ L A    +  LLG
Sbjct:    1 MARNAEKAMTALARFRQAQLEEGKVKER-RPFLASECTELPKAEKWRRQIIGEISKKVAQIQNAGLGEFRIRDLNDEINKLLREKGHWEVRIKELGGPDYGKVGPKMLDHEGKEVPGNRGYKYFGAAKDLPGVRELFEKEPLPPPRKTRAELMKAIDFEYYGYLDEDDGVIVPLEQEYEKKLRAELVEKW-KAEREARLARGEKEEEEEEEEEIN---IY-----AVTEEESDEEGNQEKAGEDGQQKFIAHVPVPSQQEIEEALVRRKKMELLQKYASETLQAQSEEAKRLLG 284
[Close]
BLAST of Pre-mRNA-splicing factor ISY1 vs. UniProt/SwissProt
Match: sp|Q20716|ISY1_CAEEL (Protein isy-1 OS=Caenorhabditis elegans OX=6239 GN=isy-1 PE=1 SV=1)

HSP 1 Score: 174.481 bits (441), Expect = 2.011e-51
Identity = 98/216 (45.37%), Postives = 136/216 (62.96%), Query Frame = 1 
Query:   49 MARSAEKKMTALARWRAVYED--KISFRERPKLASECNSLRDANMFRRQILNEVTRKITQIQNSSLGEFKLRDLNDEINRLFREKSHWEDRIVELGGTDHKRNSFLLMEKEGKALPGNWGYRYFGACKDLPGVRELFQNESDG-VSKKVRVDL----NNQYYGYMDEADGVILSIEETAE--------KEFREKLFQKSKKADKSHEEIEPNIYET 651
            MAR+AEK MTALARWR + E+  +     RP    +C +L DA  FRR+I+ + ++KIT IQN  LGEFKLRDLNDE+NRL + K  WE RI ELGGTD+++ +   ++  G+    + GY+YFGA KDLPGVRELF+  ++G   ++ R DL    +  Y+GY+D+ DG ++ +E+  E        KEF EK  QK + A  +  E   NIY+ 
Sbjct:    1 MARNAEKAMTALARWRRMKEEEERGPIARRPHDVKDCRNLSDAERFRREIVRDASKKITAIQNPGLGEFKLRDLNDEVNRLIKLKHAWEQRIRELGGTDYRKYAQKELDAIGRETGNSRGYKYFGAAKDLPGVRELFEKSTEGEEQRRHRADLLRNIDAHYFGYLDDEDGRLIPLEKLIEEKNIERINKEFAEKQAQKQQTASDAAPE---NIYKV 213
[Close]
BLAST of Pre-mRNA-splicing factor ISY1 vs. UniProt/SwissProt
Match: sp|Q54N41|ISY1_DICDI (Pre-mRNA-splicing factor ISY1 homolog OS=Dictyostelium discoideum OX=44689 GN=isy1 PE=3 SV=1)

HSP 1 Score: 156.377 bits (394), Expect = 4.599e-44
Identity = 89/183 (48.63%), Postives = 117/183 (63.93%), Query Frame = 1 
Query:   49 MARSAEKKMTALARWRAV--YEDKISFRERPKLASECNSLRDANMFRRQILNEVTRKITQIQNSSLGEFKLRDLNDEINRLFREKSHWEDRIVELGGTDHKRNSFLLMEKEGKALPGNWGYRYFGACKDLPGVRELFQNESD---GVSKKVRVDL----NNQYYGYMDEADGVILSIEETAEK 570
            MAR+ EK  + L R+  +   E K   R RP L++EC+SL DA  +RRQIL E+TR IT+IQNS+L E+K+RDLND IN+L REK HWE RI++LGG +H+  +  L + +GK   G   YRY+G  K+LPGV ELF+       G  KK R DL    ++ YYGY D+ DG +  IE   EK
Sbjct:    1 MARNEEKAKSMLNRYLQLKGTESKQEER-RPYLSNECDSLVDAEKWRRQILKEITRGITEIQNSALDEYKIRDLNDHINKLVREKGHWERRILQLGGPNHRALAPKLFDADGKEPLGTGTYRYYGEAKNLPGVAELFEKPEQTLHGTEKKTRQDLYKYIDSDYYGYRDDEDGKLEEIEREYEK 182
[Close]
BLAST of Pre-mRNA-splicing factor ISY1 vs. TrEMBL
Match: A0A5J4NS15 (Pre-mRNA-splicing factor ISY1 OS=Paragonimus westermani OX=34504 GN=DEA37_0002887 PE=4 SV=1)

HSP 1 Score: 241.506 bits (615), Expect = 1.413e-73
Identity = 153/353 (43.34%), Postives = 210/353 (59.49%), Query Frame = 1 
Query:   49 MARSAEKKMTALARWRAVYEDKIS-FRERPKLASECNSLRDANMFRRQILNEVTRKITQIQNSSLGEFKLRDLNDEINRLFREKSHWEDRIVELGGTDHKRNSFLLMEKEGKALPGNWGYRYFGACKDLPGVRELFQNESDGVSKKVRVDL----NNQYYGYMDEADGVILSIEETAEKE--FREKLFQKSKKADKS--------------HEEIEP------NIYETGDNPADIERDAIYEDL-----------------RKSITPDXXXXXXPELVTDLESRVKRFT-------------NIIVPSQQEIEXXXXXXXXXXXXXNYMSDELIADMVNSGTLLGM-DTPAAD 933
            MARSAEK MTALARWRAVY D++   ++RP +ASEC SL+DA  +RRQI+++V+RKI QIQN SLGE+KLRDLND+IN+L REK+HWED I  LGG D K  +  ++EKEGK +PGN GYRY+GA +DLPGVRELF+ E   ++KK R +L    +  YYG  DE DGVI+ +E   E+E   R+    K+++ D +               EE +       NIY +GDNPAD E   +YE +                 + S T D       E +  + SRV   T             ++ V +Q++IE  +++ KK ++L+ YMS +LIA M  +G +LG+ + P AD
Sbjct:    1 MARSAEKAMTALARWRAVYVDRVQETKKRPHIASECTSLKDALYYRRQIIHDVSRKIAQIQNPSLGEYKLRDLNDDINKLVREKAHWEDHIKTLGGPDFKAEAPKMLEKEGKEVPGNRGYRYYGASRDLPGVRELFEEEPQPIAKKSRGELMKMVDVSYYGNCDEDDGVIVPLEAAYEQEAIARKVAEWKARRNDPNLRTPAARREVDIEDQEEFDEDTSDLRNIYASGDNPADDEIMRLYEAIDADEPNDKALCAECPPVQGSGTDDNEDED--EFIAVINSRVSSKTMSSLGNVKRAFVAHVPVITQEQIEASLLAQKKREILEKYMSSDLIASMTETGEILGIEEAPGAD 351
[Close]
BLAST of Pre-mRNA-splicing factor ISY1 vs. TrEMBL
Match: A0A0X3PW00 (Pre-mRNA-splicing factor ISY1 homolog (Fragment) OS=Schistocephalus solidus OX=70667 GN=ISY1 PE=4 SV=1)

HSP 1 Score: 239.195 bits (609), Expect = 1.602e-72
Identity = 149/357 (41.74%), Postives = 204/357 (57.14%), Query Frame = 1 
Query:   49 MARSAEKKMTALARWRAVYEDKIS-FRERPKLASECNSLRDANMFRRQILNEVTRKITQIQNSSLGEFKLRDLNDEINRLFREKSHWEDRIVELGGTDHKRNSFLLMEKEGKALPGNWGYRYFGACKDLPGVRELFQNESDGVSKKVRVDLNNQ----YYGYMDEADGVILSIEETAEKEF------------------REKLFQKSKKADKSHEEIEPN-----IYETGDNPADIERDAIYEDLRKSI----TPDXXXXXXPELVTDLES-------------------------RVKR--FTNIIVPSQQEIEXXXXXXXXXXXXXNYMSDELIADMVNSGTLLGM---DTPAAD 933
            MARSAEK MTALARWRA Y DK++  ++RP  A +C  L+DA  FRRQ+++E++RKI QIQN SLGE+KLRDLNDEIN+L REK  W+ RI ELGG D+K ++  L+EK+G+  PGN GYRYFGA +DLPGVRELF+ +   + +K R +L  Q    YYG  DE DGVIL  E T E++                   R+  + +SK+  +  EE + N     IY+TGDNPAD E   +Y ++ K        D        L   L S                         ++KR    +  VPSQ+++E+ +V  +K  LL+ YM+ E +A + ++G LLG+   + PA D
Sbjct:    1 MARSAEKAMTALARWRAAYVDKVAELKKRPHFAGDCEILKDAMFFRRQVVHEISRKIAQIQNPSLGEYKLRDLNDEINKLIREKDRWDLRIKELGGPDYKADAPKLLEKDGREAPGNRGYRYFGAARDLPGVRELFEEQPQPIPRKTRGELMKQVDISYYGNCDEDDGVILPQEATYEEKLMARKAEIWKRRHEEEAAGRKAAYLRSKQYQEDQEEDDDNPDERDIYDTGDNPADTELLKLYMEIEKDAGMKKALDSEITVHASLAMALSSVEEEEEELDLAKLLKTKSGKLRPGEKLKRTFVAHTAVPSQKQMEEALVEQRKRLLLEQYMTPEFLASVSDTGKLLGVERSEQPAKD 357
[Close]
BLAST of Pre-mRNA-splicing factor ISY1 vs. TrEMBL
Match: A0A4E0RZ83 (Pre-mRNA-splicing factor ISY1 OS=Fasciola hepatica OX=6192 GN=D915_006773 PE=4 SV=1)

HSP 1 Score: 237.269 bits (604), Expect = 5.376e-72
Identity = 146/346 (42.20%), Postives = 203/346 (58.67%), Query Frame = 1 
Query:   49 MARSAEKKMTALARWRAVYEDKIS-FRERPKLASECNSLRDANMFRRQILNEVTRKITQIQNSSLGEFKLRDLNDEINRLFREKSHWEDRIVELGGTDHKRNSFLLMEKEGKALPGNWGYRYFGACKDLPGVRELFQNESDGVSKKVRVDL----NNQYYGYMDEADGVILSIEETAEKEF--REKLFQKSKKADKSHEEIEP---------------------NIYETGDNPADIERDAIYEDLRKSITPDXXXXXXPEL---------------VTDLESRVKRFT-------------NIIVPSQQEIEXXXXXXXXXXXXXNYMSDELIADMVNSGTLLGMD 918
            MARSAEK MTALARWRAVY DK+   + RP L +EC SL+DA  +RRQI+++++RKI QIQN SLGE+KLRDLND+IN+L REK+HWED I  LGG D K  +  ++EKEGK +PGN GYRY+GA +DLPGVRELF+ E   V++K R +L    +  YYG  DE DGVI+  E   E+E+  R+    K+++ D + + +                       NIY  GDNPAD E   +YE + ++           +L               +  L SRV   T             ++ V +QQ++E  +++ KK ++L+ YMS++LIA M  +G +LGM+
Sbjct:    1 MARSAEKAMTALARWRAVYVDKVQEQKRRPFLVAECTSLKDALYYRRQIIHDISRKIAQIQNPSLGEYKLRDLNDDINKLVREKAHWEDHIKTLGGPDFKAEAPKMLEKEGKEVPGNRGYRYYGAARDLPGVRELFEEEPQPVARKSRGELMKMVDVSYYGNCDEDDGVIVPQEMKYEQEYMARKVADWKARRNDPNQKGLTARMISENDDDGDIDDEDNADLRNIYAAGDNPADDEIMRLYESIDENERTASNLPTAAQLTALGAAAEEEEEDEYIAMLNSRVSGKTMSSLGNIKRTFVAHVPVYTQQQVEASLLAQKKREILEKYMSEDLIASMTQTGEILGME 346
[Close]
BLAST of Pre-mRNA-splicing factor ISY1 vs. TrEMBL
Match: Q5DCZ2 (Pre-mRNA-splicing factor ISY1 isoform 2 OS=Schistosoma japonicum OX=6182 GN=EWB00_002132 PE=2 SV=1)

HSP 1 Score: 236.884 bits (603), Expect = 8.182e-72
Identity = 147/342 (42.98%), Postives = 202/342 (59.06%), Query Frame = 1 
Query:   49 MARSAEKKMTALARWRAVYEDKIS-FRERPKLASECNSLRDANMFRRQILNEVTRKITQIQNSSLGEFKLRDLNDEINRLFREKSHWEDRIVELGGTDHKRNSFLLMEKEGKALPGNWGYRYFGACKDLPGVRELFQNESDGVSKKVRVDL----NNQYYGYMDEADGVILSIEETAEKE-----FREKLFQKSKKADKSHEEIEP---------------NIYETGDNPADIERDAIYEDLRKSITPDXXXXXXPE----LVTDLE---------------------SRVKRFTNIIVP--SQQEIEXXXXXXXXXXXXXNYMSDELIADMVNSGTLLGMD 918
            MARSAEK MTALARWRAVY D++   ++RP +ASEC  L+D+  +RRQI++E++RKI QIQN SLGE+KLRDLND+IN+L REKSHWED I  LGG D K  +  ++EKEGK +PGN GYRY+GA +DLPGVRELF+ +   V++K R +L    +  YYG  DE DGVI+  E   E+E       E  ++K+  +   +    P               NIY+ GDNPADIE   +YE + ++           E    L+ D E                     + VKR     VP  SQ++IE  +V+ +K ++L+ YMS +L+A M  +G +LG++
Sbjct:    1 MARSAEKAMTALARWRAVYVDRVQETKKRPYIASECTGLKDSLYYRRQIVHEISRKIAQIQNPSLGEYKLRDLNDDINKLIREKSHWEDHIKNLGGPDFKAEAPKMLEKEGKEVPGNRGYRYYGAARDLPGVRELFEEDPQPVARKSRGELMKLVDVSYYGNCDEDDGVIVPQEAVYEQEAIAHKVAEWKYRKTSDSQPGYSSKPPGDEFDELDEDNADTRNIYDVGDNPADIELMRLYEAIDENNEVGSVDFSNAEQLVALINDDENADELDDRTGRSSSKSMSSLSTTVKRAFVAHVPIYSQEQIEAALVAQRKREILEKYMSRDLLASMAQTGEILGIE 342
[Close]
BLAST of Pre-mRNA-splicing factor ISY1 vs. TrEMBL
Match: A0A5K4ELR8 (Uncharacterized protein OS=Schistosoma mansoni OX=6183 PE=4 SV=1)

HSP 1 Score: 236.113 bits (601), Expect = 1.359e-71
Identity = 149/342 (43.57%), Postives = 204/342 (59.65%), Query Frame = 1 
Query:   49 MARSAEKKMTALARWRAVYEDKIS-FRERPKLASECNSLRDANMFRRQILNEVTRKITQIQNSSLGEFKLRDLNDEINRLFREKSHWEDRIVELGGTDHKRNSFLLMEKEGKALPGNWGYRYFGACKDLPGVRELFQNESDGVSKKVRVDL----NNQYYGYMDEADGVILSIEETAEKE-FREKLFQ-KSKKADKSHE--EIEP----------------NIYETGDNPADIERDAIYEDLRKS-----------------ITPDXXX--------XXXPELVTDLESRVKRFTNIIVP--SQQEIEXXXXXXXXXXXXXNYMSDELIADMVNSGTLLGMD 918
            MARSAEK MTALARWRAVY D++   ++RP +ASEC  L+D+  +RRQI++E++RKI QIQN SLGE+KLRDLND+IN+L REKSHWED I  LGG D K  +  ++EKEGK +PGN GYRY+GA +DLPGVRELF+ +   V++K R +L    +  YYG  DE DGVI+  E   E+E    K+ + K +KA  S      +P                NIY+ GDNPADIE   +YE + ++                 I  D               + ++ L + VKR     VP  SQ++IE  +V+ +K ++L+ YMS +L+A M  +G +LG +
Sbjct:    1 MARSAEKAMTALARWRAVYVDRVQETKKRPYIASECTGLKDSLYYRRQIVHEISRKIAQIQNPSLGEYKLRDLNDDINKLIREKSHWEDHIKNLGGPDFKAEAPKMLEKEGKEVPGNRGYRYYGAARDLPGVRELFEEDPQPVARKSRGELMKLVDVSYYGNCDEDDGVIIPQEAVYEQEAIAYKIAEWKYRKASDSQPGYSCKPLGDEFDELDEDNADTRNIYDVGDNPADIELMRLYEAIDENNEVGSIDFSNAEQLVALINDDENTDELDNHTGSSATKSMSSLSTTVKRAFVAHVPIYSQEQIEAALVAQRKREILEKYMSSDLLASMAQTGEILGFE 342
[Close]
BLAST of Pre-mRNA-splicing factor ISY1 vs. Ensembl Cavefish
Match: isy1 (pre-mRNA-splicing factor ISY1 homolog [Source:NCBI gene;Acc:103027102])

HSP 1 Score: 211.46 bits (537), Expect = 1.972e-66
Identity = 134/297 (45.12%), Postives = 181/297 (60.94%), Query Frame = 1 
Query:   49 MARSAEKKMTALARWRAVY--EDKISFRERPKLASECNSLRDANMFRRQILNEVTRKITQIQNSSLGEFKLRDLNDEINRLFREKSHWEDRIVELGGTDHKRNSFLLMEKEGKALPGNWGYRYFGACKDLPGVRELFQNESDGVSKKVRV----DLNNQYYGYMDEADGVILSIEETAEKEFREKLFQKSKKADKSHEEIEPNIYETGDNPADIERDA-IYEDLRKSITPDXXXXXXPELVTDLESRVKRF-TNIIVPSQQEIEXXXXXXXXXXXXXNYMSDELIADMVNSGTLLGM 915
            MAR+AEK MTALAR+R     E K+  R RP LASECN L  A  +RRQI++E+++K+ QIQN+ LGEFK+RDLNDEIN+L REK HWE RI ELGG D+ R    +++ EGK +PGN GY+YFGA KDLPGVRELF+ E     +K R     D++ +YYGY DE DGV++ +E+  EK+   +  +K K      EE E  +   G      E +  IY     +I  D           + E   + F  ++ VPSQ+EIE+ +V  KK +LL+ Y S+ L+A    +  LLG+
Sbjct:    1 MARNAEKAMTALARFRQAQLEEGKVKER-RPFLASECNELPKAEKWRRQIISEISKKVAQIQNAGLGEFKIRDLNDEINKLLREKGHWEVRIKELGGPDYARVGPKMLDHEGKEVPGNRGYKYFGAAKDLPGVRELFEKEPAPPPRKTRAELMKDIDAEYYGYRDEDDGVLVPLEQEYEKQVIAEAIEKWK------EEKEARLAGVGGTKEVEEEEEYIY-----AIKDDEHSDDEMREQMEGEDGAQTFIAHVPVPSQKEIEEALVRRKKMELLQKYASESLMAQSEEAKALLGL 285
[Close]
BLAST of Pre-mRNA-splicing factor ISY1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005515.1 (pep scaffold:Pmarinus_7.0:GL490796:738:6235:1 gene:ENSPMAG00000005010.1 transcript:ENSPMAT00000005515.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 185.267 bits (469), Expect = 2.423e-58
Identity = 93/179 (51.96%), Postives = 128/179 (71.51%), Query Frame = 1 
Query:   52 ARSAEKKMTALARWRAVYEDKISFRER-PKLASECNSLRDANMFRRQILNEVTRKITQIQNSS-LGEFKLRDLNDEINRLFREKSHWEDRIVELGGTDHKRNSFLLMEKEGKALPGNWGYRYFGACKDLPGVRELFQNESDGVSKKVR----VDLNNQYYGYMDEADGVILSIEETAEK 570
            AR+AEK MTALAR+R    ++   +ER P LASEC  L+ +  +RRQI++E+++K+ QIQN++ LGEF++RDLNDEIN+L REK HWE RI+ELGG D+ R    +++ EGK +PGN GY+YFGA ++LPGVRELF  E     +K R     D++  YYGY DE D V++ +E+  EK
Sbjct:    2 ARNAEKAMTALARFRQAQLEEGKVKERRPYLASECEDLQKSEKWRRQIISEISKKVAQIQNAAGLGEFRIRDLNDEINKLLREKGHWEARILELGGPDYGRIGPKMLDHEGKEVPGNRGYKYFGAARELPGVRELFDQEPLKAPRKTRGELMKDIDADYYGYRDEDDSVLVPLEQALEK 180
[Close]
BLAST of Pre-mRNA-splicing factor ISY1 vs. Ensembl Yeast
Match: ISY1 (Member of the NineTeen Complex (NTC); NTC contains Prp19p and stabilizes U6 snRNA in catalytic forms of spliceosome containing U2, U5, and U6 snRNAs; interacts with Prp16p to modulate splicing fidelity; isy1 syf2 cells have defective spindles [Source:SGD;Acc:S000003811])

HSP 1 Score: 75.0998 bits (183), Expect = 3.073e-16
Identity = 59/173 (34.10%), Postives = 95/173 (54.91%), Query Frame = 1 
Query:   49 MARSAEKKMTALARWR-------AVYEDKISFRERPKLASECNSLRDANMFRRQILNEVTRKITQIQNSSLGEFKLRDLNDEINRLFREKSHWE---DRIVELGGTDHKR--NSFLLMEKEGKALPGNWGYRYFGACKDLPGVRELF---QNESDGVS---KKVRVDLNNQYY 513
            M+R+ +K  + L R++         Y+D  S  +RP+  S+  S+++AN ++RQ+  E+ +K T+I + SL E ++ +LNDE+N LF+E   W+   D  +    T  KR  +S +LM   GK + G    RYFG   +LP V+E     Q ++DG S   K +  D N+ YY
Sbjct:    1 MSRNVDKANSVLVRFQEQQAESAGGYKD-YSRYQRPRNVSKVKSIKEANEWKRQVSKEIKQKSTRIYDPSLNEMQIAELNDELNNLFKEWKRWQWHIDHTLMEKKTKRKRLEDSHVLMN-SGKLINGK---RYFGRALELPEVKEWLKQSQRQNDGGSINTKCIPKDRNDFYY 168
[Close]
BLAST of Pre-mRNA-splicing factor ISY1 vs. Ensembl Nematostella
Match: EDO45398 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RT52])

HSP 1 Score: 187.578 bits (475), Expect = 9.137e-58
Identity = 93/174 (53.45%), Postives = 123/174 (70.69%), Query Frame = 1 
Query:   49 MARSAEKKMTALARWRAVY--EDKISFRER-PKLASECNSLRDANMFRRQILNEVTRKITQIQNSSLGEFKLRDLNDEINRLFREKSHWEDRIVELGGTDHKRNSFLLMEKEGKALPGNWGYRYFGACKDLPGVRELFQNESDGVSKKVR----VDLNNQYYGYMDEADGVILS 549
            MAR+AEK MT L+RW  V   E     +ER P LA+EC++L++A  +R QI+ EV +K+ QIQN+ LGEF++RDLNDEIN+L REK HWEDRI ELGG D+ +    +++ EGK +PGN GY+YFGA K+LPGVRELF+ E     +K R     D++  YYGY DE DGV++ 
Sbjct:    1 MARNAEKAMTMLSRWYDVQRKEAGKGIKERRPFLATECDNLQEAEKWRHQIIREVAKKVAQIQNAGLGEFRIRDLNDEINKLLREKRHWEDRIKELGGPDYGKIGPKMLDHEGKEVPGNRGYKYFGAAKELPGVRELFEQEPPAPPRKTRGELYKDIDADYYGYRDEDDGVLVP 174
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BLAST of Pre-mRNA-splicing factor ISY1 vs. Ensembl Medaka
Match: isy1 (ISY1 splicing factor homolog [Source:NCBI gene;Acc:101158759])

HSP 1 Score: 209.534 bits (532), Expect = 1.027e-65
Identity = 132/296 (44.59%), Postives = 184/296 (62.16%), Query Frame = 1 
Query:   49 MARSAEKKMTALARWRAVY--EDKISFRERPKLASECNSLRDANMFRRQILNEVTRKITQIQNSSLGEFKLRDLNDEINRLFREKSHWEDRIVELGGTDHKRNSFLLMEKEGKALPGNWGYRYFGACKDLPGVRELFQNESDGVSKKVR----VDLNNQYYGYMDEADGVILSIEETAEKEFREKLFQKSKKADKSHEEIEPNIYETGDNPAD-IERDAIYEDLRKSITPDXXXXXXPELVTDLESRVKRFTNIIVPSQQEIEXXXXXXXXXXXXXNYMSDELIADMVNSGTLLGM 915
            MAR+AEK MTALAR+R     E K+  R RP LASEC+ L  A  +RRQI++E+++K+ QIQN+ LGEFK+RDLNDEIN+L REK HWE RI ELGG D++R    +++ EGK +PGN GY+YFGA +DLPGVRELF+ E     +K R     D++ +YYGY DE DGV+L +E   EK+   +  QK K   ++H          G+ PA   E ++IY    +    +       + + + E  V  F ++ VPSQ+E+E+ +V  KK +LL+ Y S+ L A    +  LLG+
Sbjct:    1 MARNAEKAMTALARFRQAQLEEGKVKER-RPFLASECSELPKAEKWRRQIISEISKKVAQIQNAGLGEFKIRDLNDEINKLLREKGHWEYRIKELGGPDYRRFGPRMLDHEGKEVPGNRGYKYFGAARDLPGVRELFEKEPAPEQRKTRGELMKDVDAEYYGYRDEDDGVLLPLEVQYEKQAVMEAVQKWKAEKETH--------LPGEKPAQEEEEESIYSVQNQ----EPDDDENRDELEEEEGGVTFFAHVPVPSQKEVEEALVRRKKMELLQRYASETLQAQSQEARALLGL 283
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BLAST of Pre-mRNA-splicing factor ISY1 vs. Ensembl Medaka
Match: isy1 (ISY1 splicing factor homolog [Source:NCBI gene;Acc:101158759])

HSP 1 Score: 206.838 bits (525), Expect = 1.209e-64
Identity = 131/295 (44.41%), Postives = 183/295 (62.03%), Query Frame = 1 
Query:   52 ARSAEKKMTALARWRAVY--EDKISFRERPKLASECNSLRDANMFRRQILNEVTRKITQIQNSSLGEFKLRDLNDEINRLFREKSHWEDRIVELGGTDHKRNSFLLMEKEGKALPGNWGYRYFGACKDLPGVRELFQNESDGVSKKVR----VDLNNQYYGYMDEADGVILSIEETAEKEFREKLFQKSKKADKSHEEIEPNIYETGDNPAD-IERDAIYEDLRKSITPDXXXXXXPELVTDLESRVKRFTNIIVPSQQEIEXXXXXXXXXXXXXNYMSDELIADMVNSGTLLGM 915
            AR+AEK MTALAR+R     E K+  R RP LASEC+ L  A  +RRQI++E+++K+ QIQN+ LGEFK+RDLNDEIN+L REK HWE RI ELGG D++R    +++ EGK +PGN GY+YFGA +DLPGVRELF+ E     +K R     D++ +YYGY DE DGV+L +E   EK+   +  QK K   ++H          G+ PA   E ++IY    +    +       + + + E  V  F ++ VPSQ+E+E+ +V  KK +LL+ Y S+ L A    +  LLG+
Sbjct:    4 ARNAEKAMTALARFRQAQLEEGKVKER-RPFLASECSELPKAEKWRRQIISEISKKVAQIQNAGLGEFKIRDLNDEINKLLREKGHWEYRIKELGGPDYRRFGPRMLDHEGKEVPGNRGYKYFGAARDLPGVRELFEKEPAPEQRKTRGELMKDVDAEYYGYRDEDDGVLLPLEVQYEKQAVMEAVQKWKAEKETH--------LPGEKPAQEEEEESIYSVQNQ----EPDDDENRDELEEEEGGVTFFAHVPVPSQKEVEEALVRRKKMELLQRYASETLQAQSQEARALLGL 285
The following BLAST results are available for this feature:
BLAST of Pre-mRNA-splicing factor ISY1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ISY11.633e-6445.24ISY1 splicing factor homolog [Source:HGNC Symbol;A... [more]
ISY1-RAB433.408e-6444.59ISY1-RAB43 readthrough [Source:HGNC Symbol;Acc:HGN... [more]
ISY15.447e-6155.56ISY1 splicing factor homolog [Source:HGNC Symbol;A... [more]
ISY18.971e-6042.22ISY1 splicing factor homolog [Source:HGNC Symbol;A... [more]
ISY11.463e-3854.62ISY1 splicing factor homolog [Source:HGNC Symbol;A... [more]
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BLAST of Pre-mRNA-splicing factor ISY1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2
Match NameE-valueIdentityDescription
isy-11.581e-5245.37ISY splicing factor homolog [Source:UniProtKB/TrE... [more]
isy-11.581e-5245.37ISY splicing factor homolog [Source:UniProtKB/TrE... [more]
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BLAST of Pre-mRNA-splicing factor ISY1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
CG96674.067e-5949.73gene:FBgn0037550 transcript:FBtr0081815[more]
CG96675.974e-5548.60gene:FBgn0037550 transcript:FBtr0339725[more]
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BLAST of Pre-mRNA-splicing factor ISY1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
isy12.116e-6745.08ISY1 splicing factor homolog [Source:ZFIN;Acc:ZDB-... [more]
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BLAST of Pre-mRNA-splicing factor ISY1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSXETT00000024318.11.501e-2664.79pep primary_assembly:Xenopus_tropicalis_v9.1:KV463... [more]
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BLAST of Pre-mRNA-splicing factor ISY1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Isy12.438e-6444.90ISY1 splicing factor homolog [Source:MGI Symbol;Ac... [more]
Gm451401.582e-5952.75predicted gene 45140 [Source:MGI Symbol;Acc:MGI:57... [more]
Isy16.883e-3359.60ISY1 splicing factor homolog [Source:MGI Symbol;Ac... [more]
Isy15.178e-1948.42ISY1 splicing factor homolog [Source:MGI Symbol;Ac... [more]
Isy15.643e-656.00ISY1 splicing factor homolog [Source:MGI Symbol;Ac... [more]
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BLAST of Pre-mRNA-splicing factor ISY1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q9ULR0|ISY1_HUMAN7.842e-6445.24Pre-mRNA-splicing factor ISY1 homolog OS=Homo sapi... [more]
sp|Q6AYB3|ISY1_RAT1.620e-6344.12Pre-mRNA-splicing factor ISY1 homolog OS=Rattus no... [more]
sp|Q69ZQ2|ISY1_MOUSE1.705e-6344.90Pre-mRNA-splicing factor ISY1 homolog OS=Mus muscu... [more]
sp|Q20716|ISY1_CAEEL2.011e-5145.37Protein isy-1 OS=Caenorhabditis elegans OX=6239 GN... [more]
sp|Q54N41|ISY1_DICDI4.599e-4448.63Pre-mRNA-splicing factor ISY1 homolog OS=Dictyoste... [more]
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BLAST of Pre-mRNA-splicing factor ISY1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A5J4NS151.413e-7343.34Pre-mRNA-splicing factor ISY1 OS=Paragonimus weste... [more]
A0A0X3PW001.602e-7241.74Pre-mRNA-splicing factor ISY1 homolog (Fragment) O... [more]
A0A4E0RZ835.376e-7242.20Pre-mRNA-splicing factor ISY1 OS=Fasciola hepatica... [more]
Q5DCZ28.182e-7242.98Pre-mRNA-splicing factor ISY1 isoform 2 OS=Schisto... [more]
A0A5K4ELR81.359e-7143.57Uncharacterized protein OS=Schistosoma mansoni OX=... [more]
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BLAST of Pre-mRNA-splicing factor ISY1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
isy11.972e-6645.12pre-mRNA-splicing factor ISY1 homolog [Source:NCBI... [more]
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BLAST of Pre-mRNA-splicing factor ISY1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSPMAT00000005515.12.423e-5851.96pep scaffold:Pmarinus_7.0:GL490796:738:6235:1 gene... [more]
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BLAST of Pre-mRNA-splicing factor ISY1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
ISY13.073e-1634.10Member of the NineTeen Complex (NTC); NTC contains... [more]
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BLAST of Pre-mRNA-splicing factor ISY1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO453989.137e-5853.45Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Pre-mRNA-splicing factor ISY1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
isy11.027e-6544.59ISY1 splicing factor homolog [Source:NCBI gene;Acc... [more]
isy11.209e-6444.41ISY1 splicing factor homolog [Source:NCBI gene;Acc... [more]
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BLAST of Pre-mRNA-splicing factor ISY1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 0
Match NameE-valueIdentityDescription
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30019578 ID=SMED30019578|Name=Pre-mRNA-splicing factor ISY1|organism=Schmidtea mediterranea sexual|type=transcript|length=999bp
TAAAAATTTGCATTATTTGATTTTTAATATACTGAAAAGTATTTGGAAAT
GGCTCGAAGTGCAGAAAAGAAAATGACAGCTTTAGCTCGTTGGAGAGCGG
TTTACGAGGATAAAATTTCATTCAGAGAAAGACCAAAATTAGCTTCAGAA
TGTAATAGTTTGAGAGATGCTAATATGTTTCGACGACAAATATTAAATGA
AGTGACTAGAAAAATAACTCAAATTCAAAATTCATCTTTGGGGGAATTTA
AACTCCGAGATTTAAACGATGAAATTAACAGATTATTCCGTGAAAAAAGC
CACTGGGAGGATCGAATTGTGGAATTAGGGGGTACTGATCACAAACGAAA
TTCTTTTTTGTTAATGGAAAAAGAAGGGAAAGCACTTCCTGGCAATTGGG
GCTATCGTTATTTTGGAGCCTGCAAAGATTTGCCAGGCGTCAGAGAACTA
TTTCAAAATGAATCTGATGGAGTAAGCAAAAAAGTGCGAGTGGATTTGAA
TAACCAATACTATGGCTATATGGACGAAGCGGACGGGGTGATTTTGTCAA
TAGAGGAAACTGCGGAAAAAGAATTCCGTGAGAAGTTATTCCAAAAGAGT
AAGAAAGCAGACAAATCCCACGAAGAGATTGAACCAAATATTTATGAAAC
CGGGGATAATCCGGCCGATATCGAACGGGACGCCATTTATGAAGATCTCA
GGAAAAGTATTACCCCAGATGGCGGCGGTGGTGGCGGCCCTGAATTAGTA
ACAGATCTGGAATCACGAGTAAAGAGGTTCACAAACATCATAGTTCCCAG
TCAACAGGAAATTGAGAAAGTCGTTGTGAGCTTGAAAAAGGATAAATTAT
TGAAGAACTATATGTCAGATGAGCTGATAGCAGACATGGTCAATTCTGGC
ACCCTGCTGGGCATGGATACCCCTGCCGCAGATGGCGAGCTAGACCAGCA
AGTTGAGATGGAGGATAGTTGATATTTGTAAATAAATTTTTTTGTTGCG
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protein sequence of SMED30019578-orf-1

>SMED30019578-orf-1 ID=SMED30019578-orf-1|Name=SMED30019578-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=308bp
MARSAEKKMTALARWRAVYEDKISFRERPKLASECNSLRDANMFRRQILN
EVTRKITQIQNSSLGEFKLRDLNDEINRLFREKSHWEDRIVELGGTDHKR
NSFLLMEKEGKALPGNWGYRYFGACKDLPGVRELFQNESDGVSKKVRVDL
NNQYYGYMDEADGVILSIEETAEKEFREKLFQKSKKADKSHEEIEPNIYE
TGDNPADIERDAIYEDLRKSITPDGGGGGGPELVTDLESRVKRFTNIIVP
SQQEIEKVVVSLKKDKLLKNYMSDELIADMVNSGTLLGMDTPAADGELDQ
QVEMEDS*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000429neoblast
PLANA:0002109X1 cell
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR009360Isy1
Vocabulary: biological process
TermDefinition
GO:0000350generation of catalytic spliceosome for second transesterification step
GO:0000389mRNA 3'-splice site recognition
GO:0000398mRNA splicing, via spliceosome
GO:0006283transcription-coupled nucleotide-excision repair
GO:0006397mRNA processing
GO:0008380RNA splicing
Vocabulary: cellular component
TermDefinition
GO:0000974Prp19 complex
GO:0005634nucleus
GO:0005654nucleoplasm
GO:0005681spliceosomal complex
GO:0071012catalytic step 1 spliceosome
GO:0071013catalytic step 2 spliceosome
GO:0071014post-mRNA release spliceosomal complex
GO:0071020post-spliceosomal complex
Vocabulary: molecular function
TermDefinition
GO:0003723RNA binding
GO:0005515protein binding