TIMELESS-interacting protein

Overview
NameTIMELESS-interacting protein
Smed IDSMED30019555
Length (bp)756
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of TIMELESS-interacting protein (SMED30019555) t-SNE clustered cells

Violin plots show distribution of expression levels for TIMELESS-interacting protein (SMED30019555) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of TIMELESS-interacting protein (SMED30019555) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for TIMELESS-interacting protein (SMED30019555) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30019555

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parapharyngeal regionSMED30019555h1SMcG0019236 SmedASXL_015275SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
X1 cellSMED30019555h1SMcG0019236 SmedASXL_015275SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
pharynxSMED30019555h1SMcG0019236 dd_Smed_v4_12580_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30019555h1SMcG0019236 Contig46977uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30019555h1SMcG0019236 Contig46977newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
zeta neoblastSMED30019555h1SMcG0019236 dd_Smed_v4_12580_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of TIMELESS-interacting protein vs. Ensembl Human
Match: TIPIN (TIMELESS interacting protein [Source:HGNC Symbol;Acc:HGNC:30750])

HSP 1 Score: 73.1738 bits (178), Expect = 1.355e-14
Identity = 37/79 (46.84%), Postives = 49/79 (62.03%), Query Frame = 1 
Query:  214 KLDVVKLISDRGLLALDKELSSFVFKGKGHEALDLKRLMGKYELWTQQLIGGMSFTDCIEKIQDLGHKKEVRAMMKKYR 450
            KLD  +LIS+RGL AL        FKGKGHEA DLK L+   E W  +L   + F D I++++ LG KKEV+  +K+ R
Sbjct:   66 KLDAQRLISERGLPALRHVFDKAKFKGKGHEAEDLKMLIRHMEHWAHRLFPKLQFEDFIDRVEYLGSKKEVQTCLKRIR 144
[Close]
BLAST of TIMELESS-interacting protein vs. Ensembl Human
Match: TIPIN (TIMELESS interacting protein [Source:HGNC Symbol;Acc:HGNC:30750])

HSP 1 Score: 73.1738 bits (178), Expect = 1.490e-14
Identity = 37/79 (46.84%), Postives = 49/79 (62.03%), Query Frame = 1 
Query:  214 KLDVVKLISDRGLLALDKELSSFVFKGKGHEALDLKRLMGKYELWTQQLIGGMSFTDCIEKIQDLGHKKEVRAMMKKYR 450
            KLD  +LIS+RGL AL        FKGKGHEA DLK L+   E W  +L   + F D I++++ LG KKEV+  +K+ R
Sbjct:   66 KLDAQRLISERGLPALRHVFDKAKFKGKGHEAEDLKMLIRHMEHWAHRLFPKLQFEDFIDRVEYLGSKKEVQTCLKRIR 144
[Close]
BLAST of TIMELESS-interacting protein vs. Ensembl Human
Match: TIPIN (TIMELESS interacting protein [Source:HGNC Symbol;Acc:HGNC:30750])

HSP 1 Score: 67.0106 bits (162), Expect = 1.937e-13
Identity = 35/72 (48.61%), Postives = 45/72 (62.50%), Query Frame = 1 
Query:  214 KLDVVKLISDRGLLALDKELSSFVFKGKGHEALDLKRLMGKYELWTQQLIGGMSFTDCIEKIQDLGHKKEVR 429
            KLD  +LIS+RGL AL        FKGKGHEA DLK L+   E W  +L   + F D I++++ LG KKEV+
Sbjct:   66 KLDAQRLISERGLPALRHVFDKAKFKGKGHEAEDLKMLIRHMEHWAHRLFPKLQFEDFIDRVEYLGSKKEVQ 137
[Close]
BLAST of TIMELESS-interacting protein vs. Ensembl Human
Match: TIPIN (TIMELESS interacting protein [Source:HGNC Symbol;Acc:HGNC:30750])

HSP 1 Score: 50.8322 bits (120), Expect = 8.917e-8
Identity = 26/49 (53.06%), Postives = 30/49 (61.22%), Query Frame = 1 
Query:  214 KLDVVKLISDRGLLALDKELSSFVFKGKGHEALDLKRLMGKYELWTQQL 360
            KLD  +LIS+RGL AL        FKGKGHEA DLK L+   E W  +L
Sbjct:   66 KLDAQRLISERGLPALRHVFDKAKFKGKGHEAEDLKMLIRHMEHWAHRL 114
[Close]
BLAST of TIMELESS-interacting protein vs. Ensembl Fly
Match: CG10336 (gene:FBgn0032698 transcript:FBtr0273194)

HSP 1 Score: 54.6842 bits (130), Expect = 1.803e-8
Identity = 27/87 (31.03%), Postives = 45/87 (51.72%), Query Frame = 1 
Query:  190 RRKQKPRQKLDVVKLISDRGLLALDKELSSFVFKGKGHEALDLKRLMGKYELWTQQLIGGMSFTDCIEKIQDLGHKKEVRAMMKKYR 450
            R  + PR +L V  L   RG+  ++       FKGKG+E  DL  ++ + + W  ++    +F D +  I+ LG KK ++  M +YR
Sbjct:   58 RAVRNPRPRLTVETLRGPRGIQTIEDYFKDIKFKGKGYEKTDLDEVLRRLQHWGHRMYPTYTFDDVLNNIERLGKKKPLQVHMARYR 144
[Close]
BLAST of TIMELESS-interacting protein vs. Ensembl Fly
Match: CG10336 (gene:FBgn0032698 transcript:FBtr0081095)

HSP 1 Score: 54.6842 bits (130), Expect = 1.803e-8
Identity = 27/87 (31.03%), Postives = 45/87 (51.72%), Query Frame = 1 
Query:  190 RRKQKPRQKLDVVKLISDRGLLALDKELSSFVFKGKGHEALDLKRLMGKYELWTQQLIGGMSFTDCIEKIQDLGHKKEVRAMMKKYR 450
            R  + PR +L V  L   RG+  ++       FKGKG+E  DL  ++ + + W  ++    +F D +  I+ LG KK ++  M +YR
Sbjct:   58 RAVRNPRPRLTVETLRGPRGIQTIEDYFKDIKFKGKGYEKTDLDEVLRRLQHWGHRMYPTYTFDDVLNNIERLGKKKPLQVHMARYR 144
[Close]
BLAST of TIMELESS-interacting protein vs. Ensembl Zebrafish
Match: tipin (timeless interacting protein [Source:NCBI gene;Acc:445058])

HSP 1 Score: 80.1073 bits (196), Expect = 2.769e-17
Identity = 54/145 (37.24%), Postives = 80/145 (55.17%), Query Frame = 1 
Query:  190 RRKQKPRQKLDVVKLISDRGLLALDKELSSFVFKGKGHEALDLKRLMGKYELWTQQLIGGMSFTDCIEKIQDLGHKKEVRAMMKKYRY-QGLTSE-FITDDVETVVRDIPADNFNYEDFHFDENFSTEYNAVNPADTQETEQQPI 618
            R  ++PR KLD  +LIS++GL AL        FKGKGHE  +LK LM K E W  +L   M F + I+K+++LG KKEV+  +K+ R    +T E ++ ++ E  V   P ++    D  F E+    ++   PA   E +QQ I
Sbjct:   65 RTVKRPRPKLDANRLISEKGLPALRTLFEDVKFKGKGHETENLKLLMLKMENWAHRLYPKMQFEEFIDKVENLGGKKEVQTCLKRIRLDMPITHEDYVAEEAEIQV---PEESVPSGDGGFPEDPFI-HSTPAPASLTEEQQQRI 205
[Close]
BLAST of TIMELESS-interacting protein vs. Ensembl Zebrafish
Match: tipin (timeless interacting protein [Source:NCBI gene;Acc:445058])

HSP 1 Score: 80.1073 bits (196), Expect = 2.835e-17
Identity = 54/145 (37.24%), Postives = 80/145 (55.17%), Query Frame = 1 
Query:  190 RRKQKPRQKLDVVKLISDRGLLALDKELSSFVFKGKGHEALDLKRLMGKYELWTQQLIGGMSFTDCIEKIQDLGHKKEVRAMMKKYRY-QGLTSE-FITDDVETVVRDIPADNFNYEDFHFDENFSTEYNAVNPADTQETEQQPI 618
            R  ++PR KLD  +LIS++GL AL        FKGKGHE  +LK LM K E W  +L   M F + I+K+++LG KKEV+  +K+ R    +T E ++ ++ E  V   P ++    D  F E+    ++   PA   E +QQ I
Sbjct:   65 RTVKRPRPKLDANRLISEKGLPALRTLFEDVKFKGKGHETENLKLLMLKMENWAHRLYPKMQFEEFIDKVENLGGKKEVQTCLKRIRLDMPITHEDYVAEEAEIQV---PEESVPSGDGGFPEDPFI-HSTPAPASLTEEQQQRI 205
[Close]
BLAST of TIMELESS-interacting protein vs. Ensembl Zebrafish
Match: tipin (timeless interacting protein [Source:NCBI gene;Acc:445058])

HSP 1 Score: 80.1073 bits (196), Expect = 2.835e-17
Identity = 54/145 (37.24%), Postives = 80/145 (55.17%), Query Frame = 1 
Query:  190 RRKQKPRQKLDVVKLISDRGLLALDKELSSFVFKGKGHEALDLKRLMGKYELWTQQLIGGMSFTDCIEKIQDLGHKKEVRAMMKKYRY-QGLTSE-FITDDVETVVRDIPADNFNYEDFHFDENFSTEYNAVNPADTQETEQQPI 618
            R  ++PR KLD  +LIS++GL AL        FKGKGHE  +LK LM K E W  +L   M F + I+K+++LG KKEV+  +K+ R    +T E ++ ++ E  V   P ++    D  F E+    ++   PA   E +QQ I
Sbjct:   65 RTVKRPRPKLDANRLISEKGLPALRTLFEDVKFKGKGHETENLKLLMLKMENWAHRLYPKMQFEEFIDKVENLGGKKEVQTCLKRIRLDMPITHEDYVAEEAEIQV---PEESVPSGDGGFPEDPFI-HSTPAPASLTEEQQQRI 205
[Close]
BLAST of TIMELESS-interacting protein vs. Ensembl Xenopus
Match: TIPIN (timeless interacting protein [Source:NCBI gene;Acc:100126681])

HSP 1 Score: 77.7962 bits (190), Expect = 4.765e-16
Identity = 46/129 (35.66%), Postives = 73/129 (56.59%), Query Frame = 1 
Query:  181 KSIRRK-QKPRQKLDVVKLISDRGLLALDKELSSFVFKGKGHEALDLKRLMGKYELWTQQLIGGMSFTDCIEKIQDLGHKKEVRAMMKKYR------YQGLTSEFITDDVETVVRDIPADNFNY-EDFH 543
            K  RR  ++P+ KLD  +L S RGL AL        FKGKGHEA D+K L+ + E W  +L   + F D + +++ +G+KKEV+  +KK R      ++   SE +    E    D+P+++F++ E+ H
Sbjct:   62 KPARRVVKRPQPKLDGQRLASKRGLPALRHLFEGVKFKGKGHEAEDVKLLLRQMENWAHRLFPKLQFEDFLNRLESMGNKKEVQTCLKKIRMDLPIVHEDFLSEEVVVQTEDHDTDMPSEDFSFPEELH 190
[Close]
BLAST of TIMELESS-interacting protein vs. Ensembl Mouse
Match: Tipin (timeless interacting protein [Source:MGI Symbol;Acc:MGI:1921571])

HSP 1 Score: 75.485 bits (184), Expect = 1.309e-15
Identity = 36/79 (45.57%), Postives = 50/79 (63.29%), Query Frame = 1 
Query:  214 KLDVVKLISDRGLLALDKELSSFVFKGKGHEALDLKRLMGKYELWTQQLIGGMSFTDCIEKIQDLGHKKEVRAMMKKYR 450
            KLD  +L S+RGL AL        FKGKGHEA DLK L+   E W  +L   + F D I+++++LG+KKEV+  +K+ R
Sbjct:   63 KLDATRLTSERGLPALRHVFDKTKFKGKGHEAEDLKTLIRHMEHWAHRLFPKLQFEDFIDRVENLGNKKEVQTCLKRIR 141
[Close]
BLAST of TIMELESS-interacting protein vs. Ensembl Mouse
Match: Tipin (timeless interacting protein [Source:MGI Symbol;Acc:MGI:1921571])

HSP 1 Score: 75.485 bits (184), Expect = 1.309e-15
Identity = 36/79 (45.57%), Postives = 50/79 (63.29%), Query Frame = 1 
Query:  214 KLDVVKLISDRGLLALDKELSSFVFKGKGHEALDLKRLMGKYELWTQQLIGGMSFTDCIEKIQDLGHKKEVRAMMKKYR 450
            KLD  +L S+RGL AL        FKGKGHEA DLK L+   E W  +L   + F D I+++++LG+KKEV+  +K+ R
Sbjct:   63 KLDATRLTSERGLPALRHVFDKTKFKGKGHEAEDLKTLIRHMEHWAHRLFPKLQFEDFIDRVENLGNKKEVQTCLKRIR 141
[Close]
BLAST of TIMELESS-interacting protein vs. Ensembl Mouse
Match: Tipin (timeless interacting protein [Source:MGI Symbol;Acc:MGI:1921571])

HSP 1 Score: 49.6766 bits (117), Expect = 1.390e-7
Identity = 25/49 (51.02%), Postives = 29/49 (59.18%), Query Frame = 1 
Query:  214 KLDVVKLISDRGLLALDKELSSFVFKGKGHEALDLKRLMGKYELWTQQL 360
            KLD  +L S+RGL AL        FKGKGHEA DLK L+   E W  +L
Sbjct:   63 KLDATRLTSERGLPALRHVFDKTKFKGKGHEAEDLKTLIRHMEHWAHRL 111
[Close]
BLAST of TIMELESS-interacting protein vs. UniProt/SwissProt
Match: sp|Q6DBR4|TIPIN_DANRE (TIMELESS-interacting protein OS=Danio rerio OX=7955 GN=tipin PE=2 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 2.090e-16
Identity = 54/145 (37.24%), Postives = 80/145 (55.17%), Query Frame = 1 
Query:  190 RRKQKPRQKLDVVKLISDRGLLALDKELSSFVFKGKGHEALDLKRLMGKYELWTQQLIGGMSFTDCIEKIQDLGHKKEVRAMMKKYRY-QGLTSE-FITDDVETVVRDIPADNFNYEDFHFDENFSTEYNAVNPADTQETEQQPI 618
            R  ++PR KLD  +LIS++GL AL        FKGKGHE  +LK LM K E W  +L   M F + I+K+++LG KKEV+  +K+ R    +T E ++ ++ E  V   P ++    D  F E+    ++   PA   E +QQ I
Sbjct:   65 RTVKRPRPKLDANRLISEKGLPALRTLFEDVKFKGKGHETENLKLLMLKMENWAHRLYPKMQFEEFIDKVENLGGKKEVQTCLKRIRLDMPITHEDYVAEEAEIQV---PEESVPSGDGGFPEDPFI-HSTPAPASLTEEQQQRI 205
[Close]
BLAST of TIMELESS-interacting protein vs. UniProt/SwissProt
Match: sp|Q0IHI4|TIPIN_XENLA (TIMELESS-interacting protein OS=Xenopus laevis OX=8355 GN=tipin PE=1 SV=1)

HSP 1 Score: 78.1814 bits (191), Expect = 2.203e-15
Identity = 45/126 (35.71%), Postives = 69/126 (54.76%), Query Frame = 1 
Query:  181 KSIRRK-QKPRQKLDVVKLISDRGLLALDKELSSFVFKGKGHEALDLKRLMGKYELWTQQLIGGMSFTDCIEKIQDLGHKKEVRAMMKKYR------YQGLTSEFITDDVETVVRDIPADNFNYED 537
            K  RR  ++P+ KLD  +L S RGL AL        FKGKGHE  DLK L+ + E W  +L   + F D + +++ +G+KKEV+  +KK R      +    SE +    E    D+P+++F++ D
Sbjct:   61 KPARRVVKRPQPKLDGQRLASQRGLPALRHMFDDVKFKGKGHETEDLKILLRQMENWAHRLFPKLQFEDFLNRLESMGNKKEVQTCLKKIRMDLPIVHDDFLSEEVVVQTEDHAIDMPSEDFSFPD 186
[Close]
BLAST of TIMELESS-interacting protein vs. UniProt/SwissProt
Match: sp|Q4QR88|TIPIN_RAT (TIMELESS-interacting protein OS=Rattus norvegicus OX=10116 GN=Tipin PE=2 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 8.854e-15
Identity = 36/79 (45.57%), Postives = 50/79 (63.29%), Query Frame = 1 
Query:  214 KLDVVKLISDRGLLALDKELSSFVFKGKGHEALDLKRLMGKYELWTQQLIGGMSFTDCIEKIQDLGHKKEVRAMMKKYR 450
            KLD  +L S+RGL AL        FKGKGHEA DLK L+   E W  +L   + F D I+++++LG+KKEV+  +K+ R
Sbjct:   63 KLDATRLTSERGLPALRHVFDKTKFKGKGHEAEDLKTLIRHMEHWAHRLFPKLQFEDFIDRVENLGNKKEVQTCLKRIR 141
[Close]
BLAST of TIMELESS-interacting protein vs. UniProt/SwissProt
Match: sp|Q91WA1|TIPIN_MOUSE (TIMELESS-interacting protein OS=Mus musculus OX=10090 GN=Tipin PE=1 SV=2)

HSP 1 Score: 75.485 bits (184), Expect = 9.156e-15
Identity = 36/79 (45.57%), Postives = 50/79 (63.29%), Query Frame = 1 
Query:  214 KLDVVKLISDRGLLALDKELSSFVFKGKGHEALDLKRLMGKYELWTQQLIGGMSFTDCIEKIQDLGHKKEVRAMMKKYR 450
            KLD  +L S+RGL AL        FKGKGHEA DLK L+   E W  +L   + F D I+++++LG+KKEV+  +K+ R
Sbjct:   63 KLDATRLTSERGLPALRHVFDKTKFKGKGHEAEDLKTLIRHMEHWAHRLFPKLQFEDFIDRVENLGNKKEVQTCLKRIR 141
[Close]
BLAST of TIMELESS-interacting protein vs. UniProt/SwissProt
Match: sp|Q5F416|TIPIN_CHICK (TIMELESS-interacting protein OS=Gallus gallus OX=9031 GN=TIPIN PE=2 SV=1)

HSP 1 Score: 75.0998 bits (183), Expect = 1.159e-14
Identity = 36/79 (45.57%), Postives = 51/79 (64.56%), Query Frame = 1 
Query:  214 KLDVVKLISDRGLLALDKELSSFVFKGKGHEALDLKRLMGKYELWTQQLIGGMSFTDCIEKIQDLGHKKEVRAMMKKYR 450
            KLD  +L+S+RGL AL     +  FKGKGHEA DLK L+   E W  +L   + F D I++++ LG+KKEV+  +K+ R
Sbjct:   73 KLDANRLVSERGLPALRHMFDNVKFKGKGHEAEDLKTLLRHMEHWAHRLFPKLQFDDFIDRVESLGNKKEVQTCLKRIR 151
[Close]
BLAST of TIMELESS-interacting protein vs. TrEMBL
Match: A0A182JWV7 (TIMELESS-interacting protein OS=Anopheles christyi OX=43041 PE=3 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 5.803e-18
Identity = 45/90 (50.00%), Postives = 55/90 (61.11%), Query Frame = 1 
Query:  187 IRRK--QKPRQKLDVVKLISDRGLLALDKELSSFVFKGKGHEALDLKRLMGKYELWTQQLIGGMSFTDCIEKIQDLGHKKEVRAMMKKYR 450
            ++RK  + PR  L+  KL   RG+LAL      F F GKGHEA DL RLM KYE W  +L     F DC+ KI+ LGHKK+V+  M KYR
Sbjct:   55 VKRKTIRNPRNLLNEAKLCGPRGILALKDHFKDFKFHGKGHEASDLNRLMRKYEHWAHRLYPKFHFDDCMAKIEKLGHKKQVQMYMNKYR 144
[Close]
BLAST of TIMELESS-interacting protein vs. TrEMBL
Match: A0A182WGA1 (TIMELESS-interacting protein OS=Anopheles minimus OX=112268 PE=3 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 6.974e-18
Identity = 43/90 (47.78%), Postives = 52/90 (57.78%), Query Frame = 1 
Query:  205 PRQKLDVVKLISDRGLLALDKELSSFVFKGKGHEALDLKRLMGKYELWTQQLIGGMSFTDCIEKIQDLGHKKEVRAMMKKYRYQGLTSEF 474
            PR  L+  KL   RG++AL      F F+GKGHEA DL RLM  YE W  +L     F DC+ KI+ LGHKK+V+  M KYR   L  E 
Sbjct:   61 PRNMLNEAKLCGPRGIIALKDHFKDFKFRGKGHEASDLNRLMRHYEHWAHRLYPKFHFDDCMTKIEKLGHKKQVQMYMNKYRSGQLQQEL 150
[Close]
BLAST of TIMELESS-interacting protein vs. TrEMBL
Match: A0A182MLM6 (TIMELESS-interacting protein OS=Anopheles culicifacies OX=139723 PE=3 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 8.295e-18
Identity = 43/90 (47.78%), Postives = 52/90 (57.78%), Query Frame = 1 
Query:  205 PRQKLDVVKLISDRGLLALDKELSSFVFKGKGHEALDLKRLMGKYELWTQQLIGGMSFTDCIEKIQDLGHKKEVRAMMKKYRYQGLTSEF 474
            PR  L+  KL   RG++AL      F F+GKGHEA DL RLM  YE W  +L     F DC+ KI+ LGHKK+V+  M KYR   L  E 
Sbjct:   60 PRNMLNEAKLCGPRGIIALKDHFKDFKFRGKGHEASDLNRLMRHYEHWAHRLYPKFHFDDCMAKIEKLGHKKQVQMYMNKYRSGLLQQEL 149
[Close]
BLAST of TIMELESS-interacting protein vs. TrEMBL
Match: A0A182UN36 (TIMELESS-interacting protein OS=Anopheles merus OX=30066 PE=3 SV=1)

HSP 1 Score: 90.5077 bits (223), Expect = 1.104e-17
Identity = 46/105 (43.81%), Postives = 59/105 (56.19%), Query Frame = 1 
Query:  166 AKQFLKSIRRK--QKPRQKLDVVKLISDRGLLALDKELSSFVFKGKGHEALDLKRLMGKYELWTQQLIGGMSFTDCIEKIQDLGHKKEVRAMMKKYRYQGLTSEF 474
            AK     +++K  + PR  L+  KL   RG++AL      F F GKGHEA DL RLM  YE W  +L     F DC+ KI+ LGHKK+V+  M KYR   L +E 
Sbjct:   47 AKPVAVEVKKKTVRNPRNMLNEAKLCGPRGIIALKDHFKHFKFHGKGHEASDLNRLMRNYEHWAHRLYPKFHFDDCMAKIEKLGHKKQVQMYMNKYRTGMLQAEL 151
[Close]
BLAST of TIMELESS-interacting protein vs. TrEMBL
Match: A0A182U0I4 (TIMELESS-interacting protein OS=Anopheles melas OX=34690 PE=3 SV=1)

HSP 1 Score: 90.5077 bits (223), Expect = 1.125e-17
Identity = 46/105 (43.81%), Postives = 59/105 (56.19%), Query Frame = 1 
Query:  166 AKQFLKSIRRK--QKPRQKLDVVKLISDRGLLALDKELSSFVFKGKGHEALDLKRLMGKYELWTQQLIGGMSFTDCIEKIQDLGHKKEVRAMMKKYRYQGLTSEF 474
            AK     +++K  + PR  L+  KL   RG++AL      F F GKGHEA DL RLM  YE W  +L     F DC+ KI+ LGHKK+V+  M KYR   L +E 
Sbjct:   47 AKPVAVEVKKKTVRNPRNMLNEAKLCGPRGIIALKDHFKHFKFHGKGHEASDLNRLMRNYEHWAHRLYPKFHFDDCMAKIEKLGHKKQVQMYMNKYRTGMLQAEL 151
[Close]
BLAST of TIMELESS-interacting protein vs. Ensembl Cavefish
Match: tipin (TIMELESS interacting protein [Source:NCBI gene;Acc:103029076])

HSP 1 Score: 79.337 bits (194), Expect = 5.515e-17
Identity = 51/124 (41.13%), Postives = 69/124 (55.65%), Query Frame = 1 
Query:  190 RRKQKPRQKLDVVKLISDRGLLALDKELSSFVFKGKGHEALDLKRLMGKYELWTQQLIGGMSFTDCIEKIQDLGHKKEVRAMMKKYRYQ-GLTSE-FITDDVETVVRDIPADNFNYEDFHFDEN 555
            R  ++P+ KLD  +L S+RGL AL     +  FKGKGHEA DLK L+ K E W  +L     F D I+K++ LG KKEV+  +K+ R    LT E FI +D E     +  D   + D  F E+
Sbjct:   65 RTVKRPQPKLDSQRLTSERGLPALRTLFDNVKFKGKGHEAEDLKVLLQKMENWAHRLYPKFQFEDFIDKLEVLGGKKEVQTCLKRIRLDMPLTHEDFIGNDAEAPEERLQEDKDPFGDGGFSED 188
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BLAST of TIMELESS-interacting protein vs. Ensembl Cavefish
Match: ENSAMXT00000041085.1 (TIMELESS-interacting protein-like [Source:NCBI gene;Acc:103034699])

HSP 1 Score: 58.9214 bits (141), Expect = 2.236e-10
Identity = 31/95 (32.63%), Postives = 52/95 (54.74%), Query Frame = 1 
Query:  181 KSIRRKQKPRQKLDVVKLISDRGLLALDKELSSFVFKGKGHEALDLKRLMGKYELWTQQLIGGMSFTDCIEKIQDLGHKKEVRAMMKKYRYQGLT 465
            + + +   P   L+V +L+S++G+ AL   L++  FKG+GHE  DL+ LM + E W QQL     F   I +++D+     ++ +   Y YQ L 
Sbjct:   63 EPVMQMDMPHTALNVDRLVSEKGIPALWDVLNNTSFKGQGHEEEDLQILMSEIEQWGQQLYPEEPFETFINQLEDVSDHPSIQVLFFSY-YQNLC 156
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BLAST of TIMELESS-interacting protein vs. Ensembl Cavefish
Match: ENSAMXT00000041922.1 (TIMELESS-interacting protein-like [Source:NCBI gene;Acc:103034699])

HSP 1 Score: 56.225 bits (134), Expect = 2.376e-9
Identity = 28/82 (34.15%), Postives = 47/82 (57.32%), Query Frame = 1 
Query:  205 PRQKLDVVKLISDRGLLALDKELSSFVFKGKGHEALDLKRLMGKYELWTQQLIGGMSFTDCIEKIQDLGHKKEVRAMMKKYR 450
            P   L+V +L+S++G+ AL   L++  FKG+GHE  DL+ LM + E W QQL     F   I +++D+     ++  ++  R
Sbjct:   71 PHTALNVDRLVSEKGIPALWDVLNNTSFKGQGHEEEDLQILMSEIEQWGQQLYPEEPFETFINQLEDVSDHPSIQTCVQSLR 152
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BLAST of TIMELESS-interacting protein vs. Ensembl Cavefish
Match: ENSAMXT00000031479.1 (TIMELESS-interacting protein-like [Source:NCBI gene;Acc:103034699])

HSP 1 Score: 56.225 bits (134), Expect = 2.376e-9
Identity = 28/82 (34.15%), Postives = 47/82 (57.32%), Query Frame = 1 
Query:  205 PRQKLDVVKLISDRGLLALDKELSSFVFKGKGHEALDLKRLMGKYELWTQQLIGGMSFTDCIEKIQDLGHKKEVRAMMKKYR 450
            P   L+V +L+S++G+ AL   L++  FKG+GHE  DL+ LM + E W QQL     F   I +++D+     ++  ++  R
Sbjct:   71 PHTALNVDRLVSEKGIPALWDVLNNTSFKGQGHEEEDLQILMSEIEQWGQQLYPEEPFETFINQLEDVSDHPSIQTCVQSLR 152
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BLAST of TIMELESS-interacting protein vs. Ensembl Sea Lamprey
Match: tipin (timeless interacting protein [Source:NCBI gene;Acc:445058])

HSP 1 Score: 78.9518 bits (193), Expect = 4.172e-18
Identity = 40/84 (47.62%), Postives = 53/84 (63.10%), Query Frame = 1 
Query:  199 QKPRQKLDVVKLISDRGLLALDKELSSFVFKGKGHEALDLKRLMGKYELWTQQLIGGMSFTDCIEKIQDLGHKKEVRAMMKKYR 450
            ++PR KLD  +L S+RGL AL K   +  FKGKGHE  DLK +M   E W  +L   M F D I+K++ LG K+EV+  +KK R
Sbjct:   51 KRPRLKLDAQRLSSERGLPALRKTFENVKFKGKGHEVEDLKLIMKNMEHWAHRLFPKMQFDDIIDKMEVLGSKREVQNCLKKIR 134
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BLAST of TIMELESS-interacting protein vs. Ensembl Medaka
Match: tipin (TIMELESS interacting protein [Source:NCBI gene;Acc:101157674])

HSP 1 Score: 75.0998 bits (183), Expect = 1.585e-15
Identity = 40/93 (43.01%), Postives = 58/93 (62.37%), Query Frame = 1 
Query:  178 LKSIRRK--QKPRQKLDVVKLISDRGLLALDKELSSFVFKGKGHEALDLKRLMGKYELWTQQLIGGMSFTDCIEKIQDLGHKKEVRAMMKKYR 450
            + + +RK  ++P+ KLD  +LIS+RGL AL        F+GKGHEA DL+ LM K E W  +L     F D ++K++ LG KKEV+  +K+ R
Sbjct:   55 VPAAKRKSAKRPQPKLDSNRLISERGLPALRSLFQDVHFRGKGHEAEDLRLLMQKLENWAHRLYPRFQFEDFMDKVERLGGKKEVQTCLKRIR 147
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BLAST of TIMELESS-interacting protein vs. Planmine SMEST
Match: SMESG000025543.1 (SMESG000025543.1)

HSP 1 Score: 388.267 bits (996), Expect = 1.676e-138
Identity = 215/231 (93.07%), Postives = 215/231 (93.07%), Query Frame = 1 
Query:   40 MDVFDVSVKDILMENVEEKDEQGDI*FYC*NFVNDLF*VNDEAKQFLKSIRRKQKPRQKLDVVKLISDRGLLALDKELSSFVFKGKGHEALDLKRLMGKYELWTQQLIGGMSFTDCIEKIQDLGHKKEVRAMMKKYRYQGLTSEFITDDVETVVRDIPADNFNYEDFHFDENFSTEYNAVNPADTQETEQQPIVEETPVKLTXXXXXXXXXXXXXXXXXXKSKKAANGSPL 732
            MDVFDVSVKDILMENVEEKDEQ                VNDEAKQFLKSIRRKQKPRQKLDVVKLISDRGLLALDKELSSFVFKGKGHEALDLKRLMGKYELWTQQLIGGMSFTDCIEKIQDLGHKKEVRAMMKKYRYQGLTSEFITDDVETVVRDIPADNFNYEDFHFDENFSTEYNAVNPADTQETEQQPIVEETPVKLTEELLERIERNRLRAIELLKSKKAANGSPL
Sbjct:    1 MDVFDVSVKDILMENVEEKDEQ----------------VNDEAKQFLKSIRRKQKPRQKLDVVKLISDRGLLALDKELSSFVFKGKGHEALDLKRLMGKYELWTQQLIGGMSFTDCIEKIQDLGHKKEVRAMMKKYRYQGLTSEFITDDVETVVRDIPADNFNYEDFHFDENFSTEYNAVNPADTQETEQQPIVEETPVKLTEELLERIERNRLRAIELLKSKKAANGSPL 215
The following BLAST results are available for this feature:
BLAST of TIMELESS-interacting protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 4
Match NameE-valueIdentityDescription
TIPIN1.355e-1446.84TIMELESS interacting protein [Source:HGNC Symbol;A... [more]
TIPIN1.490e-1446.84TIMELESS interacting protein [Source:HGNC Symbol;A... [more]
TIPIN1.937e-1348.61TIMELESS interacting protein [Source:HGNC Symbol;A... [more]
TIPIN8.917e-853.06TIMELESS interacting protein [Source:HGNC Symbol;A... [more]
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BLAST of TIMELESS-interacting protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of TIMELESS-interacting protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
CG103361.803e-831.03gene:FBgn0032698 transcript:FBtr0273194[more]
CG103361.803e-831.03gene:FBgn0032698 transcript:FBtr0081095[more]
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BLAST of TIMELESS-interacting protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
tipin2.769e-1737.24timeless interacting protein [Source:NCBI gene;Acc... [more]
tipin2.835e-1737.24timeless interacting protein [Source:NCBI gene;Acc... [more]
tipin2.835e-1737.24timeless interacting protein [Source:NCBI gene;Acc... [more]
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BLAST of TIMELESS-interacting protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
TIPIN4.765e-1635.66timeless interacting protein [Source:NCBI gene;Acc... [more]
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BLAST of TIMELESS-interacting protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 3
Match NameE-valueIdentityDescription
Tipin1.309e-1545.57timeless interacting protein [Source:MGI Symbol;Ac... [more]
Tipin1.309e-1545.57timeless interacting protein [Source:MGI Symbol;Ac... [more]
Tipin1.390e-751.02timeless interacting protein [Source:MGI Symbol;Ac... [more]
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BLAST of TIMELESS-interacting protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q6DBR4|TIPIN_DANRE2.090e-1637.24TIMELESS-interacting protein OS=Danio rerio OX=795... [more]
sp|Q0IHI4|TIPIN_XENLA2.203e-1535.71TIMELESS-interacting protein OS=Xenopus laevis OX=... [more]
sp|Q4QR88|TIPIN_RAT8.854e-1545.57TIMELESS-interacting protein OS=Rattus norvegicus ... [more]
sp|Q91WA1|TIPIN_MOUSE9.156e-1545.57TIMELESS-interacting protein OS=Mus musculus OX=10... [more]
sp|Q5F416|TIPIN_CHICK1.159e-1445.57TIMELESS-interacting protein OS=Gallus gallus OX=9... [more]
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BLAST of TIMELESS-interacting protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A182JWV75.803e-1850.00TIMELESS-interacting protein OS=Anopheles christyi... [more]
A0A182WGA16.974e-1847.78TIMELESS-interacting protein OS=Anopheles minimus ... [more]
A0A182MLM68.295e-1847.78TIMELESS-interacting protein OS=Anopheles culicifa... [more]
A0A182UN361.104e-1743.81TIMELESS-interacting protein OS=Anopheles merus OX... [more]
A0A182U0I41.125e-1743.81TIMELESS-interacting protein OS=Anopheles melas OX... [more]
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BLAST of TIMELESS-interacting protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 4
Match NameE-valueIdentityDescription
tipin5.515e-1741.13TIMELESS interacting protein [Source:NCBI gene;Acc... [more]
ENSAMXT00000041085.12.236e-1032.63TIMELESS-interacting protein-like [Source:NCBI gen... [more]
ENSAMXT00000041922.12.376e-934.15TIMELESS-interacting protein-like [Source:NCBI gen... [more]
ENSAMXT00000031479.12.376e-934.15TIMELESS-interacting protein-like [Source:NCBI gen... [more]
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BLAST of TIMELESS-interacting protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
tipin4.172e-1847.62timeless interacting protein [Source:NCBI gene;Acc... [more]
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BLAST of TIMELESS-interacting protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of TIMELESS-interacting protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of TIMELESS-interacting protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
tipin1.585e-1543.01TIMELESS interacting protein [Source:NCBI gene;Acc... [more]
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BLAST of TIMELESS-interacting protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000025543.11.676e-13893.07SMESG000025543.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30019555 ID=SMED30019555|Name=TIMELESS-interacting protein|organism=Schmidtea mediterranea sexual|type=transcript|length=756bp
GATATAATAGTGAAATATTTAAATTACTTTTATTTTGAAATGGATGTCTT
TGATGTTTCTGTTAAAGATATTTTGATGGAAAATGTAGAAGAAAAAGATG
AACAGGGTGATATTTAGTTTTACTGTTAAAATTTTGTTAATGATTTATTT
TAAGTAAACGATGAAGCAAAACAATTTTTAAAGTCAATTAGAAGGAAACA
GAAACCGCGACAAAAATTAGATGTAGTAAAGTTAATTAGCGATAGAGGGC
TCTTGGCTTTAGATAAAGAGTTGAGTAGTTTTGTGTTTAAAGGTAAAGGT
CATGAGGCTCTTGATTTAAAGAGACTAATGGGGAAATATGAACTTTGGAC
CCAACAATTGATTGGAGGAATGAGTTTCACTGACTGTATTGAAAAAATTC
AAGATTTGGGCCACAAAAAAGAAGTGCGGGCCATGATGAAAAAATATCGC
TATCAAGGATTGACCAGTGAATTTATTACGGACGATGTGGAAACGGTTGT
GAGAGATATTCCTGCAGACAATTTCAATTATGAAGACTTTCATTTTGACG
AAAACTTTTCAACAGAATACAATGCAGTTAATCCCGCAGACACCCAAGAA
ACGGAACAACAGCCCATTGTAGAGGAAACTCCAGTAAAACTCACCGAGGA
ACTCCTGGAGAGAATAGAAAGAAACCGACTCCGAGCAATTGAGCTGTTAA
AATCTAAAAAAGCAGCCAATGGGAGTCCATTATAATCAGCCAATCACCAT
TGAGAT
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protein sequence of SMED30019555-orf-1

>SMED30019555-orf-1 ID=SMED30019555-orf-1|Name=SMED30019555-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=136bp
MGKYELWTQQLIGGMSFTDCIEKIQDLGHKKEVRAMMKKYRYQGLTSEFI
TDDVETVVRDIPADNFNYEDFHFDENFSTEYNAVNPADTQETEQQPIVEE
TPVKLTEELLERIERNRLRAIELLKSKKAANGSPL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: biological process
TermDefinition
GO:0000076DNA replication checkpoint
GO:0007049cell cycle
GO:0006974cellular response to DNA damage stimulus
GO:0048478replication fork protection
GO:0006281DNA repair
GO:0008156negative regulation of DNA replication
GO:0051321meiotic cell cycle
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000014zeta neoblast
PLANA:0000016pharynx
PLANA:0000420parapharyngeal region
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR040038TIPIN/Csm3/Swi3
IPR012923Csm3
Vocabulary: cellular component
TermDefinition
GO:0005634nucleus
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012923Chromosome segregation in meiosis protein 3PFAMPF07962Swi3coord: 1..43
e-value: 1.2E-10
score: 41.2
NoneNo IPR availablePANTHERPTHR13220:SF11TIMELESS-INTERACTING PROTEINcoord: 1..121
IPR040038TIPIN/Csm3/Swi3PANTHERPTHR13220TIMELESS INTERACTING-RELATEDcoord: 1..121