SMED30019554

Overview
NameSMED30019554
Smed IDSMED30019554
Length (bp)1939
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30019554 (SMED30019554) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30019554 (SMED30019554) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30019554 (SMED30019554) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30019554 (SMED30019554) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30019554

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
cephalic gangliaSMED30019554h1SMcG0019478 dd_Smed_v4_13564_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30019554h1SMcG0019478 dd_Smed_v4_13564_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30019554 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.005419:19480..24376 +8570
Homology
BLAST of SMED30019554 vs. Planmine SMEST
Match: SMESG000069999.1 (SMESG000069999.1)

HSP 1 Score: 1189.87 bits (3077), Expect = 0.000e+0
Identity = 582/586 (99.32%), Postives = 583/586 (99.49%), Query Frame = 3
Query:    3 MTGVSMEYVKISAILVVQLSLIATFSVKTSSKSQLTLNSIFEIQIQPNLSEPTLAILVGDTMLIQITDNIGLIKQREIFYYGIKDTIENEIKGDYMNIKRWEVNYNDSSINLFIIFHKNSNFHLFEIYFIDAPSKEILKKKIKNLRLAKRIYKRTIELFHPPTLSFTVNSVLGAHSQWEINDTIIAICRAFDGIPNEIPKFENYQGFSNISSTFSLERNERMINMTVGTENHMNLLQCRAGSEVKVHTIKVFHHPTELFIKPKLKLVYSNTTFQCYAKDGFPTPTMEWFLVEGPANHSNYYELHGNRSYLFIKDNCPNHSKWKFKCKLTHMIRNSSRTLVKMISFFFENNTIGQCNLDCLHVRLLEAEYSMKKTDQSACDVKSNRLDILFLKKEVSSIREHIYQFKKDFIISIAQSKLEDKILHSRINLLKNETFLSNSLLFSMLPANKSAIDPNPYGNFLKERNVSIDDFIQIDKSLDAFKQLPWKEISQLSKHLKEITNNYQTNVKIIKLERNLIRNLSHIQNDFRGKYDLLKLENTALKYRLELLRSRNNEMNKAFLLKMTNFEKKLAKIPEMKMISNDSILL 1760
            MTGVSMEYVKISAILVVQLSLIATFSVKTS KSQLTLNSIFEIQIQPNLSEPT+AILVGDTMLIQITDNIGLIKQREIFYYGIKDTIENEIKGDYMNIKRWEVNYNDSSINLFIIFHKNSNFHLFEIYFIDAPSKEILKKKIKNLRLAKRIYKRTIELFHPPTLSFTVNSVLGAHSQWEINDTIIAICRAFDGIPNEIPKFENYQGFSNISSTFSLERNERMINMTVGTENHMN LQCRAGSEVKVHTIKVFHHPTELFIKPKLKLVYSNTTFQCYAKDGFPTPTMEWFLVEGPANHSNYYELHGNRSYLFIKDNCPNHSKWKFKCKLTHMIRNSSRTLVKMISFFFENNTIGQCNLDCLHVRLLEAEYSMKKTDQSACDVKSNRLDILFLKKEVSSIREHIYQFKKDFIISIAQSKLEDKILHSRINLLKNETFLSNSLLFSMLPANKSAIDPNPYGNFLKERNVSIDDFIQIDKSLDAFKQLPWKEISQLSKHLKEITNNYQTNVKIIKLERNLIRNLSHIQNDFR KYDLLKLENTALKYRLELLRSRNNEMNKAFLLKMTNFEKKLAKIPEMKMISNDSILL
Sbjct:    1 MTGVSMEYVKISAILVVQLSLIATFSVKTSYKSQLTLNSIFEIQIQPNLSEPTVAILVGDTMLIQITDNIGLIKQREIFYYGIKDTIENEIKGDYMNIKRWEVNYNDSSINLFIIFHKNSNFHLFEIYFIDAPSKEILKKKIKNLRLAKRIYKRTIELFHPPTLSFTVNSVLGAHSQWEINDTIIAICRAFDGIPNEIPKFENYQGFSNISSTFSLERNERMINMTVGTENHMNPLQCRAGSEVKVHTIKVFHHPTELFIKPKLKLVYSNTTFQCYAKDGFPTPTMEWFLVEGPANHSNYYELHGNRSYLFIKDNCPNHSKWKFKCKLTHMIRNSSRTLVKMISFFFENNTIGQCNLDCLHVRLLEAEYSMKKTDQSACDVKSNRLDILFLKKEVSSIREHIYQFKKDFIISIAQSKLEDKILHSRINLLKNETFLSNSLLFSMLPANKSAIDPNPYGNFLKERNVSIDDFIQIDKSLDAFKQLPWKEISQLSKHLKEITNNYQTNVKIIKLERNLIRNLSHIQNDFRRKYDLLKLENTALKYRLELLRSRNNEMNKAFLLKMTNFEKKLAKIPEMKMISNDSILL 586          
BLAST of SMED30019554 vs. Planmine SMEST
Match: SMESG000070014.1 (SMESG000070014.1)

HSP 1 Score: 250.366 bits (638), Expect = 1.053e-74
Identity = 123/348 (35.34%), Postives = 198/348 (56.90%), Query Frame = 3
Query:   66 IATFSVKTSSKSQLTLNSIFEIQIQPNLSEPTLAILVGDTMLIQITDNIGLIKQREIFYYGIKDTIENEIKGDYMNIKRWEVNYNDSSINLFIIFHKNSNFHLFEIYFIDAPSKEILKKKIKNLRLAKRIYKRTIELFHPPTLSFTVNSVLGAHSQWEINDTIIAICRAFDGIPNEIPKFENYQGFSNISSTFSLERNERMINMTVGTENHMNLLQCRAGSEVKVHTIKVFHHPTELFIKPKLKLVYSNTTFQCYAKDGFPTPTMEWFLVEGPANHSNYYELHGNRSYLFIKDNCPNHSKWKFKCKLTHMIRNSSRTLVKMISFFFENNTIGQCNLDCLHVRLLEAEY 1109
            + + + K  SK    +N  F+ QI P   +  L + + DTM+IQI D  GL+K++ IF+YG  + ++ E  G +M I +WE+N N S+I++ IIF   SNF++ EIY++   SK ++KK +K+L LA RI++ +I L  P  +    +        W +NDT+   CRA+ G+P  IP FEN++ F+ I+S+F  E+NERMI   V  E+H+  ++C AG+  K   IKV H P +++IK        N    C    GFPTP+++W L  GP + S +Y +  + + LFI +  P +SKW+F+C+++  + +      +  S   E   I  CN DC + ++   E+
Sbjct:   20 VESLTAKALSKFPFLMNETFQFQILPKTKKGNLTVSLLDTMVIQIVDMAGLVKEKAIFHYGFHEKLDQEFHGSFMKIVKWELNSNASAIDISIIFQPKSNFYIIEIYYVCPQSKSMMKKSLKDLNLAYRIFRNSINLLSPSKVDLRASPNQQNDFIWNLNDTVTVTCRAYGGMPKRIPLFENFKSFAIINSSFFPEKNERMIKFLVRREDHLKTIKCGAGNISKSQIIKVKHRPLQVYIKQN-----KNKEIDCSVVGGFPTPSIKWALTSGPQDASKFYNITSSGTRLFINEKSPLNSKWEFECQISQRLGSFITHTKRTFSTVVEKPKIS-CNTDCANKKITNLEH 361          
BLAST of SMED30019554 vs. Planmine SMEST
Match: SMESG000070014.1 (SMESG000070014.1)

HSP 1 Score: 249.98 bits (637), Expect = 1.953e-74
Identity = 123/348 (35.34%), Postives = 198/348 (56.90%), Query Frame = 3
Query:   66 IATFSVKTSSKSQLTLNSIFEIQIQPNLSEPTLAILVGDTMLIQITDNIGLIKQREIFYYGIKDTIENEIKGDYMNIKRWEVNYNDSSINLFIIFHKNSNFHLFEIYFIDAPSKEILKKKIKNLRLAKRIYKRTIELFHPPTLSFTVNSVLGAHSQWEINDTIIAICRAFDGIPNEIPKFENYQGFSNISSTFSLERNERMINMTVGTENHMNLLQCRAGSEVKVHTIKVFHHPTELFIKPKLKLVYSNTTFQCYAKDGFPTPTMEWFLVEGPANHSNYYELHGNRSYLFIKDNCPNHSKWKFKCKLTHMIRNSSRTLVKMISFFFENNTIGQCNLDCLHVRLLEAEY 1109
            + + + K  SK    +N  F+ QI P   +  L + + DTM+IQI D  GL+K++ IF+YG  + ++ E  G +M I +WE+N N S+I++ IIF   SNF++ EIY++   SK ++KK +K+L LA RI++ +I L  P  +    +        W +NDT+   CRA+ G+P  IP FEN++ F+ I+S+F  E+NERMI   V  E+H+  ++C AG+  K   IKV H P +++IK        N    C    GFPTP+++W L  GP + S +Y +  + + LFI +  P +SKW+F+C+++  + +      +  S   E   I  CN DC + ++   E+
Sbjct:   20 VESLTAKALSKFPFLMNETFQFQILPKTKKGNLTVSLLDTMVIQIVDMAGLVKEKAIFHYGFHEKLDQEFHGSFMKIVKWELNSNASAIDISIIFQPKSNFYIIEIYYVCPQSKSMMKKSLKDLNLAYRIFRNSINLLSPSKVDLRASPNQQNDFIWNLNDTVTVTCRAYGGMPKRIPLFENFKSFAIINSSFFPEKNERMIKFLVRREDHLKTIKCGAGNISKSQIIKVKHRPLQVYIKQN-----KNKEIDCSVVGGFPTPSIKWALTSGPQDASKFYNITSSGTRLFINEKSPLNSKWEFECQISQRLGSFITHTKRTFSTVVEKPKIS-CNTDCANKKITNLEH 361          
BLAST of SMED30019554 vs. Planmine SMEST
Match: SMESG000014496.1 (SMESG000014496.1)

HSP 1 Score: 230.335 bits (586), Expect = 4.250e-67
Identity = 120/328 (36.59%), Postives = 186/328 (56.71%), Query Frame = 3
Query:  111 LNSIFEIQIQPNLSEPTLAILVGDTMLIQITDNIGLIKQREIFYYGIKDTIENEIKGDYMNIKRWEVNYNDSSINLFIIFHKNSNFHLFEIYFIDAPSKEILKKKIKNLRLAKRIYKRTIELFHPPTLSFTVNSVLGAHSQWEINDTIIAICRAFDGIPNEIPKFENYQGFSNISSTFSLERNERMINMTVGTENHMNLLQCRAGSEVKVHTIKVFHHPTELFIKPKLKLVY-SNTTFQCYAKDGFPTPTMEWFLVEGPANHSNYYELHGNRSYLFIKDNCPNHSKWKFKCKLTHMIRNSSRTLVKMISFFFENNTIGQCNLDCLHVR 1091
            L + F+IQI+P  +   L+    DTM+I+I       +++ I  YG +D I+ EI G +M+IK WE N++   IN+ I+F + ++    EI++  AP+K   +K +KNL LAKR+  + IELF P  L+ ++  +     +  IND ++A CRAF GIP E PKFE++   + +   ++   NE  +N+     +H+    C AG+  + +TI++ HHPT L+I+P +K  + SN+T  C+ KDG+P P + W L+EGP N   +Y   G    + I  N P  SKW FKC +  M+ N+ + L K ISF    N +  C   CL  R
Sbjct:   35 LKNTFDIQIKPQANGTKLSDDPSDTMVIRIYSFNFTEEEKSILNYGSQDAIDEEILGKFMDIKVWEKNFSSLRINISIMFKRGAHNKYLEIFYFSAPNKFQRRKYLKNLSLAKRLLYQKIELFMPSQLNLSMYPLNVDRKEIIINDMVMASCRAFGGIPQEKPKFEDHVHLNVMEKIYNKTTNEIRMNLRASLSDHLTNFSCSAGNVTETYTIRIHHHPTALYIQPIIKKRFRSNSTIICFTKDGYPDPEINWQLIEGPPNADRFYNFPGKGDQIHINSNSPVGSKWTFKCTMKSMLVNTKKMLNKTISFAIAKNPMEICGEKCLSSR 362          
BLAST of SMED30019554 vs. Planmine SMEST
Match: SMESG000070072.1 (SMESG000070072.1)

HSP 1 Score: 223.016 bits (567), Expect = 5.736e-65
Identity = 128/395 (32.41%), Postives = 211/395 (53.42%), Query Frame = 3
Query:   45 LVVQLSLIATFSVKTSSKSQLTLNSIFEIQIQPNLSEPTLAILVGDTMLIQITDNIGLIKQREIFYYGIKDTIENEIKGDYMNIKRWEVNYNDSSINLFIIFHKNSNFHLFEIYFIDAPSKEILKKKIKNLRLAKRIYKRTIELFHPPTLSFTVNSVLGAHSQWEINDTIIAICRAFDGIPNEIPKFENYQGFSNISSTFSLERNERMINMTVGTENHMNLLQCRAGSEVKVHTIKVFHHPTELFIKPKLKLVYSNTTFQCYAKDGFPTPTMEWFLVEGPANHSNYYELHGNRSYLFIKDNCPNHSKWKFKCKLTHMIRNSSRTLVKMISFFFENNTIGQCNLDCLHVRLLEAEYSMKKTDQSACDVKSNRLDILFLKKEVSSIREHIYQFKKDF 1229
            L++  S I  F VK+ SK    +   F + IQ       L+    DTM I+I D      +R    YG+ + +++E KG  M++K WE N   +++N+ IIF++ ++    EI++  A +    KK +KNL LAKR++   I+LF P TL+ +V  +     +  IND ++A C+AF GIP E P+FE++   +     ++   NE  +N+     +H+    C AG+  +  TI++ HHPT L+I+P  K   SN+T +CY  DG+P P + W L+EGP N + +Y +      ++I  N    SKW F+C +   + N+++ L K ISF    N +  C   CL+ R+ E E ++K+  +   D+K     +  ++KE   IR+    F+ +F
Sbjct:   13 LILAQSQIPLF-VKSVSKFPHLIGDAFSLDIQSRTENGKLSSDPSDTMAIRIVDLNFTEIERAFLVYGVLNFMDDEFKGKLMDVKVWEKNPTLTAMNISIIFNRAAHNKYLEIFYFSASNALERKKYLKNLSLAKRLFHELIDLFIPSTLTLSVLPIKVDRREIIINDMVMASCKAFGGIPQEKPRFEDHAHLNVTEIIYNKTANEIRMNLRASLTDHLTNFSCVAGNVTETFTIRIHHHPTALYIQPIKKRFQSNSTIKCYTNDGYPDPEINWQLIEGPPNANLFYNIPPKGDQIYINSNSQTGSKWTFRCTMKSFLVNTNKILNKTISFAITRNPMEICGEKCLNSRIFEIEETVKENVRLNEDIKLILNQMEAMRKEHQEIRKAFLYFQWNF 406          
The following BLAST results are available for this feature:
BLAST of SMED30019554 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019554 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019554 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019554 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019554 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019554 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019554 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019554 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019554 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019554 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019554 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019554 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019554 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019554 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000069999.10.000e+099.32SMESG000069999.1[more]
SMESG000070014.11.053e-7435.34SMESG000070014.1[more]
SMESG000070014.11.953e-7435.34SMESG000070014.1[more]
SMESG000014496.14.250e-6736.59SMESG000014496.1[more]
SMESG000070072.15.736e-6532.41SMESG000070072.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30019554 ID=SMED30019554|Name=SMED30019554|organism=Schmidtea mediterranea sexual|type=transcript|length=1939bp
AAATGACCGGTGTCAGCATGGAATATGTAAAAATATCAGCAATTTTGGTT
GTTCAGCTCAGTCTAATAGCAACATTCAGTGTAAAAACTTCATCCAAATC
TCAGCTGACCCTCAATTCAATTTTTGAAATCCAAATCCAGCCTAATCTAA
GTGAGCCAACATTGGCGATTCTGGTCGGTGACACGATGCTCATACAGATC
ACAGATAACATCGGATTGATCAAGCAGCGGGAGATTTTTTATTACGGTAT
TAAAGATACCATTGAAAATGAAATAAAGGGAGATTATATGAACATAAAGA
GATGGGAAGTAAACTACAATGATTCATCCATCAATTTATTCATAATCTTC
CATAAAAACTCCAACTTCCATTTGTTTGAAATTTATTTCATTGATGCCCC
TTCAAAAGAAATATTAAAAAAGAAAATCAAAAATTTGCGTTTAGCTAAAC
GCATCTATAAAAGAACGATAGAATTATTCCACCCCCCTACACTGTCGTTT
ACAGTGAACTCGGTATTGGGAGCTCATTCTCAGTGGGAAATTAATGATAC
CATCATAGCCATATGTAGGGCCTTCGACGGTATTCCAAACGAAATTCCAA
AATTTGAAAACTATCAAGGATTTTCTAATATAAGTAGCACATTTTCCTTA
GAACGAAATGAAAGAATGATCAACATGACGGTTGGGACAGAGAACCACAT
GAATCTGTTGCAATGTCGAGCTGGATCAGAGGTAAAAGTTCATACAATAA
AAGTTTTTCATCACCCAACCGAGCTGTTCATAAAACCAAAATTAAAGCTT
GTATACTCAAATACAACATTTCAATGTTATGCCAAAGACGGTTTTCCCAC
ACCAACCATGGAATGGTTCCTCGTTGAAGGTCCAGCAAACCATTCGAATT
ATTACGAACTCCACGGGAACCGCTCTTATTTATTTATAAAAGACAATTGT
CCTAATCACTCTAAATGGAAATTTAAATGTAAACTTACCCATATGATTCG
GAATTCAAGTCGAACTTTAGTTAAAATGATCTCTTTTTTCTTTGAAAACA
ATACAATTGGTCAATGCAATCTTGATTGTTTGCATGTTAGGCTACTTGAA
GCTGAATATTCAATGAAAAAAACCGACCAATCAGCTTGCGATGTCAAAAG
CAATCGATTAGATATATTGTTTTTGAAAAAGGAAGTTTCAAGCATCCGTG
AACATATCTATCAATTTAAAAAGGATTTCATTATTTCAATTGCACAATCA
AAACTGGAAGATAAAATTTTGCATAGTCGAATCAATCTGTTAAAGAATGA
AACTTTCTTATCAAATTCCTTGCTATTCTCGATGCTTCCTGCAAACAAAT
CTGCAATTGATCCAAATCCATATGGCAACTTTTTGAAGGAAAGAAATGTG
TCCATTGATGATTTTATTCAAATTGATAAAAGTCTGGATGCATTTAAACA
ATTGCCATGGAAAGAGATTAGTCAATTATCAAAGCATTTGAAAGAAATCA
CGAATAATTATCAAACGAATGTTAAGATTATTAAATTGGAAAGAAATCTC
ATTCGAAATCTCTCTCATATTCAAAACGATTTCCGAGGAAAATACGATTT
ATTGAAATTGGAAAATACAGCCTTGAAATACCGGTTGGAATTACTGCGAT
CCAGAAATAACGAAATGAATAAAGCATTTCTTTTAAAGATGACAAATTTT
GAAAAAAAACTCGCGAAAATTCCTGAAATGAAAATGATTTCTAATGATTC
GATTCTTCTATGATTTGACAAGCCAATCAATGCCTTGCGGCCTTTGAATG
CATGTACAATTCTCGTTTTCTGATGCTTTATTGGTTGTTGTAAATCTAAT
TTAGAATGATGCCATCCAATCATAATTTGACTTGCCATTTCATTATTGTT
ATGATGATGATGTTGTTTGTAAGTTTCATTAACAATCAT
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protein sequence of SMED30019554-orf-1

>SMED30019554-orf-1 ID=SMED30019554-orf-1|Name=SMED30019554-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=123bp
MIVNETYKQHHHHNNNEMASQIMIGWHHSKLDLQQPIKHQKTRIVHAFKG
RKALIGLSNHRRIESLEIIFISGIFASFFSKFVIFKRNALFISLFLDRSN
SNRYFKAVFSNFNKSYFPRKSF*
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protein sequence of SMED30019554-orf-2

>SMED30019554-orf-2 ID=SMED30019554-orf-2|Name=SMED30019554-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=587bp
MTGVSMEYVKISAILVVQLSLIATFSVKTSSKSQLTLNSIFEIQIQPNLS
EPTLAILVGDTMLIQITDNIGLIKQREIFYYGIKDTIENEIKGDYMNIKR
WEVNYNDSSINLFIIFHKNSNFHLFEIYFIDAPSKEILKKKIKNLRLAKR
IYKRTIELFHPPTLSFTVNSVLGAHSQWEINDTIIAICRAFDGIPNEIPK
FENYQGFSNISSTFSLERNERMINMTVGTENHMNLLQCRAGSEVKVHTIK
VFHHPTELFIKPKLKLVYSNTTFQCYAKDGFPTPTMEWFLVEGPANHSNY
YELHGNRSYLFIKDNCPNHSKWKFKCKLTHMIRNSSRTLVKMISFFFENN
TIGQCNLDCLHVRLLEAEYSMKKTDQSACDVKSNRLDILFLKKEVSSIRE
HIYQFKKDFIISIAQSKLEDKILHSRINLLKNETFLSNSLLFSMLPANKS
AIDPNPYGNFLKERNVSIDDFIQIDKSLDAFKQLPWKEISQLSKHLKEIT
NNYQTNVKIIKLERNLIRNLSHIQNDFRGKYDLLKLENTALKYRLELLRS
RNNEMNKAFLLKMTNFEKKLAKIPEMKMISNDSILL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR013783Ig-like_fold
IPR007110Ig-like_dom
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 135..155
IPR007110Immunoglobulin-like domainPROSITEPS50835IG_LIKEcoord: 255..344
score: 7.595