SMED30019541

Overview
NameSMED30019541
Smed IDSMED30019541
Length (bp)1761
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30019541 (SMED30019541) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30019541 (SMED30019541) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30019541 (SMED30019541) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30019541 (SMED30019541) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30019541

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 41

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parapharyngeal regionSMED30019541h1SMcG0018856 SmedASXL_074463SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30019541h1SMcG0018856 SmedASXL_019902SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30019541h1SMcG0018856 SmedASXL_025596SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30019541h1SMcG0018856 SmedASXL_015921SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30019541h1SMcG0018856 SmedASXL_015920SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30019541h1SMcG0018856 SmedASXL_015919SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
muscle cellSMED30019541h1SMcG0018856 dd_Smed_v4_1647_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30019541h1SMcG0018856 dd_Smed_v4_10746_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30019541h1SMcG0018856 dd_Smed_v4_10746_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30019541h1SMcG0018855 dd_Smed_v4_10746_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30019541h1SMcG0018855 dd_Smed_v4_10746_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30019541h1SMcG0018856 dd_Smed_v4_651_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30019541h1SMcG0018856 dd_Smed_v4_651_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30019541h1SMcG0018856 dd_Smed_v4_651_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30019541h1SMcG0018854 Contig14068uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30019541h1SMcG0018854 Contig14068newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30019541h1SMcG0018855 Contig10526uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30019541h1SMcG0018855 Contig10526newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30019541h1SMcG0018856 Contig10526uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30019541h1SMcG0018856 Contig10526newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30019541h1SMcG0018853 Contig14068uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30019541h1SMcG0018853 Contig14068newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30019541h1SMcG0018855 Contig14068uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30019541h1SMcG0018855 Contig14068newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30019541h1SMcG0018856 Contig14068uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30019541h1SMcG0018856 Contig14068newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30019541h1SMcG0018856 Contig46457uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30019541h1SMcG0018856 Contig46457newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30019541h1SMcG0018856 Contig45454newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30019541h1SMcG0018856 Contig45454uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
parapharyngeal regionSMED30019541h1SMcG0018856 dd_Smed_v6_1647_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30019541h1SMcG0018856 dd_Smed_v6_10746_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30019541h1SMcG0018855 dd_Smed_v6_10746_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30019541h1SMcG0018856 dd_Smed_v6_12625_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30019541h1SMcG0018856 dd_Smed_v6_651_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
head regionSMED30019541h1SMcG0018856 OX_Smed_1.0.05555ox_Smed_v2PMID:24238224
Kao et al., 2013
whole organism asexual adult RNA-sequencing evidence
reproductive organSMED30019541h1SMcG0018856 Contig46551newmark_estsPMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30019541h1SMcG0018856 Contig46551uc_Smed_v2PMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
epidermisSMED30019541h1SMcG0018856 dd_Smed_v4_1647_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
Category 2 cellSMED30019541h1SMcG0018856 dd_Smed_v4_651_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
gutSMED30019541h1SMcG0018856 dd_Smed_v4_651_0_1dd_Smed_v4PMID:30962434
Cote et al., 2019
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30019541 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000191:184520..190400 +8458
v31.000191:165820..170910 +8453
v31.000191:191625..195303 +8133
v31.000191:163014..165456 +5562
Homology
BLAST of SMED30019541 vs. Planmine SMEST
Match: SMESG000025545.1 (SMESG000025545.1)

HSP 1 Score: 754.977 bits (1948), Expect = 0.000e+0
Identity = 468/469 (99.79%), Postives = 468/469 (99.79%), Query Frame = 1
Query:  166 CAIIDGLVSQYLINDRQITIDGKLDSRVSLVRNSGGPGLELSSNNRPSFKIDFTENGIREKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAPCKLVNGMVSNVFIADHQXXXXXXXXXXXXXXXXXXAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQLIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLXXXXXXXXXXXXPSKRLVDLEVIGCFKNITGCFLDGKYYQNHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVXXXXXXXXXXXXXXXTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKYDSKTRTVINAKCMSYTVDESTCGTTP 1572
            CAIIDGLVSQYLINDRQITIDGKLDSRVSLVRNSGGPGLELSSNNRPSFKIDFTENGIREKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCFEKKTEGTSTLTESTTTKEKVTTKSSSKSTTISTSTLPPITTTAPCKLVNGMVSNVFIADHQISINSKSDKNNKNNNINNAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPF HQLIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLISVEIVIEKEEVPSKRLVDLEVIGCFKNITGCFLDGKYYQNHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVNENLECCNCLPNKPNTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKYDSKTRTVINAKCMSYTVDESTCGTTP
Sbjct: 5620 CAIIDGLVSQYLINDRQITIDGKLDSRVSLVRNSGGPGLELSSNNRPSFKIDFTENGIREKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCFEKKTEGTSTLTESTTTKEKVTTKSSSKSTTISTSTLPPITTTAPCKLVNGMVSNVFIADHQISINSKSDKNNKNNNINNAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFTHQLIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLISVEIVIEKEEVPSKRLVDLEVIGCFKNITGCFLDGKYYQNHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVNENLECCNCLPNKPNTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKYDSKTRTVINAKCMSYTVDESTCGTTP 6088          

HSP 2 Score: 744.191 bits (1920), Expect = 0.000e+0
Identity = 486/497 (97.79%), Postives = 489/497 (98.39%), Query Frame = 1
Query:  166 CAIIDGLVSQYLINDRQITIDGKLDSRVSLVRNSGGPGLELSSNNRPSFKIDFTENGIREKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAPCKLVNGMVSNVFIADHQXXXXXXXXXXXXXXXXXXAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQLIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLXXXXXXXXXXXXPSKRLVDLEVIGCFKNITGCFLDGKYYQNHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVXXXXXXXXXXXXXXXTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKYDSKTRTVINAKCMSYTVDESTCGTTPXXXXXXXXXXXXXXXXXXXXXXSVTSAR 1656
            CAIIDGLVSQYLINDRQITIDGK+DSRVSLVRNSGGPGLELSSNN PSFKIDFT+NGI EKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCFEKKTEGTSTLTESTTTKEKVTTKSSSKSTTISTSTLPPITTTAPC LVNGMVSNVFIADHQISINSKSDKNN  NN   AYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQLIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLISVEIVIEKEEVPSKRLVDLEVIGCFKNITGCFLDGKYYQNHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVNENLECCNCLPNKPNTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKY+SKTRTVINAKCMSYTVDESTCGTTPKESTSSRITTTISKTPIKTETPSVTSAR
Sbjct: 4628 CAIIDGLVSQYLINDRQITIDGKVDSRVSLVRNSGGPGLELSSNNLPSFKIDFTDNGIHEKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCFEKKTEGTSTLTESTTTKEKVTTKSSSKSTTISTSTLPPITTTAPCTLVNGMVSNVFIADHQISINSKSDKNNNINN---AYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQLIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLISVEIVIEKEEVPSKRLVDLEVIGCFKNITGCFLDGKYYQNHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVNENLECCNCLPNKPNTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKYNSKTRTVINAKCMSYTVDESTCGTTPKESTSSRITTTISKTPIKTETPSVTSAR 5121          

HSP 3 Score: 695.656 bits (1794), Expect = 0.000e+0
Identity = 468/496 (94.35%), Postives = 472/496 (95.16%), Query Frame = 1
Query:  166 CAIIDGLVSQYLINDRQITIDGKLDSRVSLVRNSGGPGLELSSNNRPSFKIDFTENGIREKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAPCKLVNGMVSNVFIADHQXXXXXXXXXXXXXXXXXXAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQLIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLXXXXXXXXXXXXPSKRLVDLEVIGCFKNITGCFLDGKYYQNHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVXXXXXXXXXXXXXXXTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKYDSKTRTVINAKCMSYTVDESTCGTTPXXXXXXXXXXXXXXXXXXXXXXSVTSA 1653
            CAIIDGLVSQYLINDRQITIDGKLDSRVSLVRNSGGPGLELSSNNRPSFK+DFT+NGIREKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCFEKKTEGTSTLTESTTTKEKVTTKSSSKSTTISTSTLPPITTTAPCKLVNGMVSNVFIADHQISINSKSDKNN  NN   AYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQLIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLISVEIVIE+EEVPSKRLVDLE                   NHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVNENLECCNCLPNKPNTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKYDSKTRTVINAKCMSYTVDESTCGTTPKESTSSRITTTISKTPIKTETPSVT+A
Sbjct: 2033 CAIIDGLVSQYLINDRQITIDGKLDSRVSLVRNSGGPGLELSSNNRPSFKMDFTDNGIREKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCFEKKTEGTSTLTESTTTKEKVTTKSSSKSTTISTSTLPPITTTAPCKLVNGMVSNVFIADHQISINSKSDKNNNINN---AYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQLIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLISVEIVIEREEVPSKRLVDLE-------------------NHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVNENLECCNCLPNKPNTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKYDSKTRTVINAKCMSYTVDESTCGTTPKESTSSRITTTISKTPIKTETPSVTTA 2506          

HSP 4 Score: 499.204 bits (1284), Expect = 4.783e-157
Identity = 323/344 (93.90%), Postives = 324/344 (94.19%), Query Frame = 1
Query:  679 APCKLVNGMVSNVFIADHQXXXXXXXXXXXXXXXXXXAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQ------------------LIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLXXXXXXXXXXXXPSKRLVDLEVIGCFKNITGCFLDGKYYQNHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVXXXXXXXXXXXXXXXTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKYDSKTRTVINAKCMSYTVDESTCGTTPXXXXXXXXXXXXXXXXXXXXXXSVTSAR 1656
            APCKLVNGMVSNVFIADHQISINSKSDKNNKNNNINNAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQ                  LIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLISVEIVIEKEEVPSKRLVDLEVIGCFKNITGCFL GKYYQNHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVNENLECCNCLPNKPNTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKY+SKTRTVIN KCMSYTVDESTCGTTPKESTSSRITTTISKTPIKTETPSVTSAR
Sbjct: 1463 APCKLVNGMVSNVFIADHQISINSKSDKNNKNNNINNAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQVEMFLSIIVIINECFGIQLIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLISVEIVIEKEEVPSKRLVDLEVIGCFKNITGCFLHGKYYQNHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVNENLECCNCLPNKPNTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKYNSKTRTVINTKCMSYTVDESTCGTTPKESTSSRITTTISKTPIKTETPSVTSAR 1806          

HSP 5 Score: 205.682 bits (522), Expect = 4.426e-56
Identity = 125/287 (43.55%), Postives = 180/287 (62.72%), Query Frame = 1
Query:  679 APCKLVNGMVSNVFIADHQXXXXXXXXXXXXXXXXXXAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQLIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLXXXXXXXXXXXXPSKRL-VDLEVIGCFKNITGCFLDGKYYQNHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVXXXXXXXXXXXXXXXTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKYDSKTRTVINAKCMS 1536
             PCK++NG+VS  +I D Q+ IN+K D             LR+SDSSGL I+   S    ID T+NGNREIPFI ++I  +Q+   E    D+    +++          +++ + L S    +        R+ + ++++GCFKN+TGC ++G+ Y+NHQ +N+++   +C++ +C+CSDHK+SCR+    + C KD IK CSKV+ N+ECCNC+ NK  TYCQ+DYLCNPHCG+CL+E D WGK     +   CVCK GYQSFI +YEE+  SKT   +N KCMS
Sbjct: 6193 TPCKVINGLVSEFYIVDQQVKINNKYDSRVSL--------LRESDSSGLIISISSSLVFSIDFTENGNREIPFIEKIIFSEQIGKIEFTAIDKTNTVTKQTKTFTTFPPVLELNKPLKSFIFQVTDSLDGRDRVAIKIQILGCFKNVTGCMVNGRQYENHQLVNSSINSGQCATDMCFCSDHKVSCRSSKLPEKCPKDYIKSCSKVSGNVECCNCVQNKTETYCQVDYLCNPHCGECLNERDSWGKEIATDHPENCVCKDGYQSFIQSYEERAGSKTIYHVNVKCMS 6471          

HSP 6 Score: 155.992 bits (393), Expect = 2.056e-39
Identity = 73/129 (56.59%), Postives = 97/129 (75.19%), Query Frame = 1
Query:  166 CAIIDGLVSQYLINDRQITIDGKLDSRVSLVRNSGGPGLELSSNNRPSFKIDFTENGIREKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCF 552
            CAIIDGLVSQ+LIND QI ++GK D RVS +R S   G E+S  + P+F +DFT+NG+RE  FIYQ+I TGGV+T  +  D +++  ++K+EP V  IN+NIKTL   ++SF  ++EKIFLKIQILGCF
Sbjct: 3756 CAIIDGLVSQFLINDHQIMVNGKPDPRVSFLRFSNEIGFEISLVSLPTFTVDFTDNGLRETMFIYQIILTGGVKTANILTDGVQNMTTFKEEPAVFQINKNIKTLGLELMSFLTSKEKIFLKIQILGCF 3884          

HSP 7 Score: 85.8853 bits (211), Expect = 5.796e-17
Identity = 56/134 (41.79%), Postives = 85/134 (63.43%), Query Frame = 1
Query:  166 CAIIDGLVSQYLINDRQITIDGKLDSRVSLVRNSGGPGLELSSNNRPSFKIDFTENGIREKPFIYQVIFT---GGVETIRVRKDN--LESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCF 552
            C +I+GLVS++ I D+Q+ I+ K DSRVSL+R S   GL +S ++   F IDFTENG RE PFI ++IF+   G +E   + K N   +   ++   P VL +N+ +K+  F V    + ++++ +KIQILGCF
Sbjct: 6195 CKVINGLVSEFYIVDQQVKINNKYDSRVSLLRESDSSGLIISISSSLVFSIDFTENGNREIPFIEKIIFSEQIGKIEFTAIDKTNTVTKQTKTFTTFPPVLELNKPLKSFIFQVTDSLDGRDRVAIKIQILGCF 6328          

HSP 8 Score: 51.2174 bits (121), Expect = 2.389e-6
Identity = 29/75 (38.67%), Postives = 42/75 (56.00%), Query Frame = 1
Query:  166 CAIIDGLVSQYLINDRQITIDGKLDSRVSL--------VRNSGGPGLELSSNNRPSFKIDFTENGIREKPFIYQV 366
            C +++G+VS   I D QI+I+ K D             +R S   GLE+++    + KID T+NG RE PFI+QV
Sbjct: 1465 CKLVNGMVSNVFIADHQISINSKSDKNNKNNNINNAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQV 1539          

HSP 9 Score: 49.2914 bits (116), Expect = 9.234e-6
Identity = 30/78 (38.46%), Postives = 44/78 (56.41%), Query Frame = 1
Query:  679 APCKLVNGMVSNVFIADHQXXXXXXXXXXXXXXXXXXAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQLI 912
             PC +++G+VS   I D QI+I+ K D             +R S   GLE+++    + K+D TDNG RE PFI+Q+I
Sbjct: 2031 PPCAIIDGLVSQYLINDRQITIDGKLDSRVSL--------VRNSGGPGLELSSNNRPSFKMDFTDNGIREKPFIYQVI 2100          
BLAST of SMED30019541 vs. Planmine SMEST
Match: SMESG000025545.1 (SMESG000025545.1)

HSP 1 Score: 754.977 bits (1948), Expect = 0.000e+0
Identity = 468/469 (99.79%), Postives = 468/469 (99.79%), Query Frame = 1
Query:  166 CAIIDGLVSQYLINDRQITIDGKLDSRVSLVRNSGGPGLELSSNNRPSFKIDFTENGIREKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAPCKLVNGMVSNVFIADHQXXXXXXXXXXXXXXXXXXAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQLIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLXXXXXXXXXXXXPSKRLVDLEVIGCFKNITGCFLDGKYYQNHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVXXXXXXXXXXXXXXXTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKYDSKTRTVINAKCMSYTVDESTCGTTP 1572
            CAIIDGLVSQYLINDRQITIDGKLDSRVSLVRNSGGPGLELSSNNRPSFKIDFTENGIREKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCFEKKTEGTSTLTESTTTKEKVTTKSSSKSTTISTSTLPPITTTAPCKLVNGMVSNVFIADHQISINSKSDKNNKNNNINNAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPF HQLIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLISVEIVIEKEEVPSKRLVDLEVIGCFKNITGCFLDGKYYQNHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVNENLECCNCLPNKPNTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKYDSKTRTVINAKCMSYTVDESTCGTTP
Sbjct: 5639 CAIIDGLVSQYLINDRQITIDGKLDSRVSLVRNSGGPGLELSSNNRPSFKIDFTENGIREKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCFEKKTEGTSTLTESTTTKEKVTTKSSSKSTTISTSTLPPITTTAPCKLVNGMVSNVFIADHQISINSKSDKNNKNNNINNAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFTHQLIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLISVEIVIEKEEVPSKRLVDLEVIGCFKNITGCFLDGKYYQNHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVNENLECCNCLPNKPNTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKYDSKTRTVINAKCMSYTVDESTCGTTP 6107          

HSP 2 Score: 750.355 bits (1936), Expect = 0.000e+0
Identity = 487/496 (98.19%), Postives = 491/496 (98.99%), Query Frame = 1
Query:  166 CAIIDGLVSQYLINDRQITIDGKLDSRVSLVRNSGGPGLELSSNNRPSFKIDFTENGIREKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAPCKLVNGMVSNVFIADHQXXXXXXXXXXXXXXXXXXAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQLIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLXXXXXXXXXXXXPSKRLVDLEVIGCFKNITGCFLDGKYYQNHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVXXXXXXXXXXXXXXXTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKYDSKTRTVINAKCMSYTVDESTCGTTPXXXXXXXXXXXXXXXXXXXXXXSVTSA 1653
            CAIIDGLVSQYLINDRQITIDGKLDSRVSLVRNSGGPGLELSSNNRPSFK+DFT+NGIREKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCFEKKTEGTSTLTESTTTKEKVTTKSSSKSTTISTSTLPPITTTAPCKLVNGMVSNVFIADHQISINSKSDKNN  NN   AYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQLIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLISVEIVIE+EEVPSKRLVDLEVIGCFKNITGCFLDGKYYQNHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVNENLECCNCLPNKPNTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKYDSKTRTVINAKCMSYTVDESTCGTTPKESTSSRITTTISKTPIKTETPSVT+A
Sbjct: 2033 CAIIDGLVSQYLINDRQITIDGKLDSRVSLVRNSGGPGLELSSNNRPSFKMDFTDNGIREKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCFEKKTEGTSTLTESTTTKEKVTTKSSSKSTTISTSTLPPITTTAPCKLVNGMVSNVFIADHQISINSKSDKNNNINN---AYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQLIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLISVEIVIEREEVPSKRLVDLEVIGCFKNITGCFLDGKYYQNHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVNENLECCNCLPNKPNTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKYDSKTRTVINAKCMSYTVDESTCGTTPKESTSSRITTTISKTPIKTETPSVTTA 2525          

HSP 3 Score: 744.191 bits (1920), Expect = 0.000e+0
Identity = 486/497 (97.79%), Postives = 489/497 (98.39%), Query Frame = 1
Query:  166 CAIIDGLVSQYLINDRQITIDGKLDSRVSLVRNSGGPGLELSSNNRPSFKIDFTENGIREKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAPCKLVNGMVSNVFIADHQXXXXXXXXXXXXXXXXXXAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQLIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLXXXXXXXXXXXXPSKRLVDLEVIGCFKNITGCFLDGKYYQNHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVXXXXXXXXXXXXXXXTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKYDSKTRTVINAKCMSYTVDESTCGTTPXXXXXXXXXXXXXXXXXXXXXXSVTSAR 1656
            CAIIDGLVSQYLINDRQITIDGK+DSRVSLVRNSGGPGLELSSNN PSFKIDFT+NGI EKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCFEKKTEGTSTLTESTTTKEKVTTKSSSKSTTISTSTLPPITTTAPC LVNGMVSNVFIADHQISINSKSDKNN  NN   AYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQLIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLISVEIVIEKEEVPSKRLVDLEVIGCFKNITGCFLDGKYYQNHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVNENLECCNCLPNKPNTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKY+SKTRTVINAKCMSYTVDESTCGTTPKESTSSRITTTISKTPIKTETPSVTSAR
Sbjct: 4647 CAIIDGLVSQYLINDRQITIDGKVDSRVSLVRNSGGPGLELSSNNLPSFKIDFTDNGIHEKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCFEKKTEGTSTLTESTTTKEKVTTKSSSKSTTISTSTLPPITTTAPCTLVNGMVSNVFIADHQISINSKSDKNNNINN---AYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQLIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLISVEIVIEKEEVPSKRLVDLEVIGCFKNITGCFLDGKYYQNHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVNENLECCNCLPNKPNTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKYNSKTRTVINAKCMSYTVDESTCGTTPKESTSSRITTTISKTPIKTETPSVTSAR 5140          

HSP 4 Score: 499.204 bits (1284), Expect = 4.924e-157
Identity = 323/344 (93.90%), Postives = 324/344 (94.19%), Query Frame = 1
Query:  679 APCKLVNGMVSNVFIADHQXXXXXXXXXXXXXXXXXXAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQ------------------LIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLXXXXXXXXXXXXPSKRLVDLEVIGCFKNITGCFLDGKYYQNHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVXXXXXXXXXXXXXXXTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKYDSKTRTVINAKCMSYTVDESTCGTTPXXXXXXXXXXXXXXXXXXXXXXSVTSAR 1656
            APCKLVNGMVSNVFIADHQISINSKSDKNNKNNNINNAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQ                  LIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLISVEIVIEKEEVPSKRLVDLEVIGCFKNITGCFL GKYYQNHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVNENLECCNCLPNKPNTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKY+SKTRTVIN KCMSYTVDESTCGTTPKESTSSRITTTISKTPIKTETPSVTSAR
Sbjct: 1463 APCKLVNGMVSNVFIADHQISINSKSDKNNKNNNINNAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQVEMFLSIIVIINECFGIQLIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLISVEIVIEKEEVPSKRLVDLEVIGCFKNITGCFLHGKYYQNHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVNENLECCNCLPNKPNTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKYNSKTRTVINTKCMSYTVDESTCGTTPKESTSSRITTTISKTPIKTETPSVTSAR 1806          

HSP 5 Score: 205.682 bits (522), Expect = 4.427e-56
Identity = 125/287 (43.55%), Postives = 180/287 (62.72%), Query Frame = 1
Query:  679 APCKLVNGMVSNVFIADHQXXXXXXXXXXXXXXXXXXAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQLIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLXXXXXXXXXXXXPSKRL-VDLEVIGCFKNITGCFLDGKYYQNHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVXXXXXXXXXXXXXXXTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKYDSKTRTVINAKCMS 1536
             PCK++NG+VS  +I D Q+ IN+K D             LR+SDSSGL I+   S    ID T+NGNREIPFI ++I  +Q+   E    D+    +++          +++ + L S    +        R+ + ++++GCFKN+TGC ++G+ Y+NHQ +N+++   +C++ +C+CSDHK+SCR+    + C KD IK CSKV+ N+ECCNC+ NK  TYCQ+DYLCNPHCG+CL+E D WGK     +   CVCK GYQSFI +YEE+  SKT   +N KCMS
Sbjct: 6212 TPCKVINGLVSEFYIVDQQVKINNKYDSRVSL--------LRESDSSGLIISISSSLVFSIDFTENGNREIPFIEKIIFSEQIGKIEFTAIDKTNTVTKQTKTFTTFPPVLELNKPLKSFIFQVTDSLDGRDRVAIKIQILGCFKNVTGCMVNGRQYENHQLVNSSINSGQCATDMCFCSDHKVSCRSSKLPEKCPKDYIKSCSKVSGNVECCNCVQNKTETYCQVDYLCNPHCGECLNERDSWGKEIATDHPENCVCKDGYQSFIQSYEERAGSKTIYHVNVKCMS 6490          

HSP 6 Score: 155.992 bits (393), Expect = 2.038e-39
Identity = 73/129 (56.59%), Postives = 97/129 (75.19%), Query Frame = 1
Query:  166 CAIIDGLVSQYLINDRQITIDGKLDSRVSLVRNSGGPGLELSSNNRPSFKIDFTENGIREKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCF 552
            CAIIDGLVSQ+LIND QI ++GK D RVS +R S   G E+S  + P+F +DFT+NG+RE  FIYQ+I TGGV+T  +  D +++  ++K+EP V  IN+NIKTL   ++SF  ++EKIFLKIQILGCF
Sbjct: 3775 CAIIDGLVSQFLINDHQIMVNGKPDPRVSFLRFSNEIGFEISLVSLPTFTVDFTDNGLRETMFIYQIILTGGVKTANILTDGVQNMTTFKEEPAVFQINKNIKTLGLELMSFLTSKEKIFLKIQILGCF 3903          

HSP 7 Score: 85.8853 bits (211), Expect = 5.747e-17
Identity = 56/134 (41.79%), Postives = 85/134 (63.43%), Query Frame = 1
Query:  166 CAIIDGLVSQYLINDRQITIDGKLDSRVSLVRNSGGPGLELSSNNRPSFKIDFTENGIREKPFIYQVIFT---GGVETIRVRKDN--LESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCF 552
            C +I+GLVS++ I D+Q+ I+ K DSRVSL+R S   GL +S ++   F IDFTENG RE PFI ++IF+   G +E   + K N   +   ++   P VL +N+ +K+  F V    + ++++ +KIQILGCF
Sbjct: 6214 CKVINGLVSEFYIVDQQVKINNKYDSRVSLLRESDSSGLIISISSSLVFSIDFTENGNREIPFIEKIIFSEQIGKIEFTAIDKTNTVTKQTKTFTTFPPVLELNKPLKSFIFQVTDSLDGRDRVAIKIQILGCF 6347          

HSP 8 Score: 51.2174 bits (121), Expect = 2.409e-6
Identity = 29/75 (38.67%), Postives = 42/75 (56.00%), Query Frame = 1
Query:  166 CAIIDGLVSQYLINDRQITIDGKLDSRVSL--------VRNSGGPGLELSSNNRPSFKIDFTENGIREKPFIYQV 366
            C +++G+VS   I D QI+I+ K D             +R S   GLE+++    + KID T+NG RE PFI+QV
Sbjct: 1465 CKLVNGMVSNVFIADHQISINSKSDKNNKNNNINNAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQV 1539          

HSP 9 Score: 49.6766 bits (117), Expect = 8.926e-6
Identity = 30/78 (38.46%), Postives = 44/78 (56.41%), Query Frame = 1
Query:  679 APCKLVNGMVSNVFIADHQXXXXXXXXXXXXXXXXXXAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQLI 912
             PC +++G+VS   I D QI+I+ K D             +R S   GLE+++    + K+D TDNG RE PFI+Q+I
Sbjct: 2031 PPCAIIDGLVSQYLINDRQITIDGKLDSRVSL--------VRNSGGPGLELSSNNRPSFKMDFTDNGIREKPFIYQVI 2100          
BLAST of SMED30019541 vs. Planmine SMEST
Match: SMESG000025545.1 (SMESG000025545.1)

HSP 1 Score: 754.977 bits (1948), Expect = 0.000e+0
Identity = 468/469 (99.79%), Postives = 468/469 (99.79%), Query Frame = 1
Query:  166 CAIIDGLVSQYLINDRQITIDGKLDSRVSLVRNSGGPGLELSSNNRPSFKIDFTENGIREKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAPCKLVNGMVSNVFIADHQXXXXXXXXXXXXXXXXXXAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQLIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLXXXXXXXXXXXXPSKRLVDLEVIGCFKNITGCFLDGKYYQNHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVXXXXXXXXXXXXXXXTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKYDSKTRTVINAKCMSYTVDESTCGTTP 1572
            CAIIDGLVSQYLINDRQITIDGKLDSRVSLVRNSGGPGLELSSNNRPSFKIDFTENGIREKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCFEKKTEGTSTLTESTTTKEKVTTKSSSKSTTISTSTLPPITTTAPCKLVNGMVSNVFIADHQISINSKSDKNNKNNNINNAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPF HQLIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLISVEIVIEKEEVPSKRLVDLEVIGCFKNITGCFLDGKYYQNHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVNENLECCNCLPNKPNTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKYDSKTRTVINAKCMSYTVDESTCGTTP
Sbjct: 5618 CAIIDGLVSQYLINDRQITIDGKLDSRVSLVRNSGGPGLELSSNNRPSFKIDFTENGIREKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCFEKKTEGTSTLTESTTTKEKVTTKSSSKSTTISTSTLPPITTTAPCKLVNGMVSNVFIADHQISINSKSDKNNKNNNINNAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFTHQLIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLISVEIVIEKEEVPSKRLVDLEVIGCFKNITGCFLDGKYYQNHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVNENLECCNCLPNKPNTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKYDSKTRTVINAKCMSYTVDESTCGTTP 6086          

HSP 2 Score: 744.191 bits (1920), Expect = 0.000e+0
Identity = 486/497 (97.79%), Postives = 489/497 (98.39%), Query Frame = 1
Query:  166 CAIIDGLVSQYLINDRQITIDGKLDSRVSLVRNSGGPGLELSSNNRPSFKIDFTENGIREKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAPCKLVNGMVSNVFIADHQXXXXXXXXXXXXXXXXXXAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQLIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLXXXXXXXXXXXXPSKRLVDLEVIGCFKNITGCFLDGKYYQNHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVXXXXXXXXXXXXXXXTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKYDSKTRTVINAKCMSYTVDESTCGTTPXXXXXXXXXXXXXXXXXXXXXXSVTSAR 1656
            CAIIDGLVSQYLINDRQITIDGK+DSRVSLVRNSGGPGLELSSNN PSFKIDFT+NGI EKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCFEKKTEGTSTLTESTTTKEKVTTKSSSKSTTISTSTLPPITTTAPC LVNGMVSNVFIADHQISINSKSDKNN  NN   AYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQLIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLISVEIVIEKEEVPSKRLVDLEVIGCFKNITGCFLDGKYYQNHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVNENLECCNCLPNKPNTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKY+SKTRTVINAKCMSYTVDESTCGTTPKESTSSRITTTISKTPIKTETPSVTSAR
Sbjct: 4626 CAIIDGLVSQYLINDRQITIDGKVDSRVSLVRNSGGPGLELSSNNLPSFKIDFTDNGIHEKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCFEKKTEGTSTLTESTTTKEKVTTKSSSKSTTISTSTLPPITTTAPCTLVNGMVSNVFIADHQISINSKSDKNNNINN---AYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQLIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLISVEIVIEKEEVPSKRLVDLEVIGCFKNITGCFLDGKYYQNHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVNENLECCNCLPNKPNTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKYNSKTRTVINAKCMSYTVDESTCGTTPKESTSSRITTTISKTPIKTETPSVTSAR 5119          

HSP 3 Score: 695.656 bits (1794), Expect = 0.000e+0
Identity = 468/496 (94.35%), Postives = 472/496 (95.16%), Query Frame = 1
Query:  166 CAIIDGLVSQYLINDRQITIDGKLDSRVSLVRNSGGPGLELSSNNRPSFKIDFTENGIREKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAPCKLVNGMVSNVFIADHQXXXXXXXXXXXXXXXXXXAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQLIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLXXXXXXXXXXXXPSKRLVDLEVIGCFKNITGCFLDGKYYQNHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVXXXXXXXXXXXXXXXTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKYDSKTRTVINAKCMSYTVDESTCGTTPXXXXXXXXXXXXXXXXXXXXXXSVTSA 1653
            CAIIDGLVSQYLINDRQITIDGKLDSRVSLVRNSGGPGLELSSNNRPSFK+DFT+NGIREKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCFEKKTEGTSTLTESTTTKEKVTTKSSSKSTTISTSTLPPITTTAPCKLVNGMVSNVFIADHQISINSKSDKNN  NN   AYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQLIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLISVEIVIE+EEVPSKRLVDLE                   NHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVNENLECCNCLPNKPNTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKYDSKTRTVINAKCMSYTVDESTCGTTPKESTSSRITTTISKTPIKTETPSVT+A
Sbjct: 2031 CAIIDGLVSQYLINDRQITIDGKLDSRVSLVRNSGGPGLELSSNNRPSFKMDFTDNGIREKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCFEKKTEGTSTLTESTTTKEKVTTKSSSKSTTISTSTLPPITTTAPCKLVNGMVSNVFIADHQISINSKSDKNNNINN---AYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQLIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLISVEIVIEREEVPSKRLVDLE-------------------NHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVNENLECCNCLPNKPNTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKYDSKTRTVINAKCMSYTVDESTCGTTPKESTSSRITTTISKTPIKTETPSVTTA 2504          

HSP 4 Score: 499.204 bits (1284), Expect = 4.783e-157
Identity = 323/344 (93.90%), Postives = 324/344 (94.19%), Query Frame = 1
Query:  679 APCKLVNGMVSNVFIADHQXXXXXXXXXXXXXXXXXXAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQ------------------LIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLXXXXXXXXXXXXPSKRLVDLEVIGCFKNITGCFLDGKYYQNHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVXXXXXXXXXXXXXXXTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKYDSKTRTVINAKCMSYTVDESTCGTTPXXXXXXXXXXXXXXXXXXXXXXSVTSAR 1656
            APCKLVNGMVSNVFIADHQISINSKSDKNNKNNNINNAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQ                  LIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLISVEIVIEKEEVPSKRLVDLEVIGCFKNITGCFL GKYYQNHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVNENLECCNCLPNKPNTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKY+SKTRTVIN KCMSYTVDESTCGTTPKESTSSRITTTISKTPIKTETPSVTSAR
Sbjct: 1461 APCKLVNGMVSNVFIADHQISINSKSDKNNKNNNINNAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQVEMFLSIIVIINECFGIQLIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLISVEIVIEKEEVPSKRLVDLEVIGCFKNITGCFLHGKYYQNHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVNENLECCNCLPNKPNTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKYNSKTRTVINTKCMSYTVDESTCGTTPKESTSSRITTTISKTPIKTETPSVTSAR 1804          

HSP 5 Score: 205.682 bits (522), Expect = 4.426e-56
Identity = 125/287 (43.55%), Postives = 180/287 (62.72%), Query Frame = 1
Query:  679 APCKLVNGMVSNVFIADHQXXXXXXXXXXXXXXXXXXAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQLIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLXXXXXXXXXXXXPSKRL-VDLEVIGCFKNITGCFLDGKYYQNHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVXXXXXXXXXXXXXXXTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKYDSKTRTVINAKCMS 1536
             PCK++NG+VS  +I D Q+ IN+K D             LR+SDSSGL I+   S    ID T+NGNREIPFI ++I  +Q+   E    D+    +++          +++ + L S    +        R+ + ++++GCFKN+TGC ++G+ Y+NHQ +N+++   +C++ +C+CSDHK+SCR+    + C KD IK CSKV+ N+ECCNC+ NK  TYCQ+DYLCNPHCG+CL+E D WGK     +   CVCK GYQSFI +YEE+  SKT   +N KCMS
Sbjct: 6191 TPCKVINGLVSEFYIVDQQVKINNKYDSRVSL--------LRESDSSGLIISISSSLVFSIDFTENGNREIPFIEKIIFSEQIGKIEFTAIDKTNTVTKQTKTFTTFPPVLELNKPLKSFIFQVTDSLDGRDRVAIKIQILGCFKNVTGCMVNGRQYENHQLVNSSINSGQCATDMCFCSDHKVSCRSSKLPEKCPKDYIKSCSKVSGNVECCNCVQNKTETYCQVDYLCNPHCGECLNERDSWGKEIATDHPENCVCKDGYQSFIQSYEERAGSKTIYHVNVKCMS 6469          

HSP 6 Score: 155.992 bits (393), Expect = 2.056e-39
Identity = 73/129 (56.59%), Postives = 97/129 (75.19%), Query Frame = 1
Query:  166 CAIIDGLVSQYLINDRQITIDGKLDSRVSLVRNSGGPGLELSSNNRPSFKIDFTENGIREKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCF 552
            CAIIDGLVSQ+LIND QI ++GK D RVS +R S   G E+S  + P+F +DFT+NG+RE  FIYQ+I TGGV+T  +  D +++  ++K+EP V  IN+NIKTL   ++SF  ++EKIFLKIQILGCF
Sbjct: 3754 CAIIDGLVSQFLINDHQIMVNGKPDPRVSFLRFSNEIGFEISLVSLPTFTVDFTDNGLRETMFIYQIILTGGVKTANILTDGVQNMTTFKEEPAVFQINKNIKTLGLELMSFLTSKEKIFLKIQILGCF 3882          

HSP 7 Score: 85.8853 bits (211), Expect = 5.796e-17
Identity = 56/134 (41.79%), Postives = 85/134 (63.43%), Query Frame = 1
Query:  166 CAIIDGLVSQYLINDRQITIDGKLDSRVSLVRNSGGPGLELSSNNRPSFKIDFTENGIREKPFIYQVIFT---GGVETIRVRKDN--LESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCF 552
            C +I+GLVS++ I D+Q+ I+ K DSRVSL+R S   GL +S ++   F IDFTENG RE PFI ++IF+   G +E   + K N   +   ++   P VL +N+ +K+  F V    + ++++ +KIQILGCF
Sbjct: 6193 CKVINGLVSEFYIVDQQVKINNKYDSRVSLLRESDSSGLIISISSSLVFSIDFTENGNREIPFIEKIIFSEQIGKIEFTAIDKTNTVTKQTKTFTTFPPVLELNKPLKSFIFQVTDSLDGRDRVAIKIQILGCF 6326          

HSP 8 Score: 51.2174 bits (121), Expect = 2.389e-6
Identity = 29/75 (38.67%), Postives = 42/75 (56.00%), Query Frame = 1
Query:  166 CAIIDGLVSQYLINDRQITIDGKLDSRVSL--------VRNSGGPGLELSSNNRPSFKIDFTENGIREKPFIYQV 366
            C +++G+VS   I D QI+I+ K D             +R S   GLE+++    + KID T+NG RE PFI+QV
Sbjct: 1463 CKLVNGMVSNVFIADHQISINSKSDKNNKNNNINNAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQV 1537          

HSP 9 Score: 49.2914 bits (116), Expect = 9.234e-6
Identity = 30/78 (38.46%), Postives = 44/78 (56.41%), Query Frame = 1
Query:  679 APCKLVNGMVSNVFIADHQXXXXXXXXXXXXXXXXXXAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQLI 912
             PC +++G+VS   I D QI+I+ K D             +R S   GLE+++    + K+D TDNG RE PFI+Q+I
Sbjct: 2029 PPCAIIDGLVSQYLINDRQITIDGKLDSRVSL--------VRNSGGPGLELSSNNRPSFKMDFTDNGIREKPFIYQVI 2098          
BLAST of SMED30019541 vs. Planmine SMEST
Match: SMESG000025545.1 (SMESG000025545.1)

HSP 1 Score: 754.977 bits (1948), Expect = 0.000e+0
Identity = 468/469 (99.79%), Postives = 468/469 (99.79%), Query Frame = 1
Query:  166 CAIIDGLVSQYLINDRQITIDGKLDSRVSLVRNSGGPGLELSSNNRPSFKIDFTENGIREKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAPCKLVNGMVSNVFIADHQXXXXXXXXXXXXXXXXXXAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQLIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLXXXXXXXXXXXXPSKRLVDLEVIGCFKNITGCFLDGKYYQNHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVXXXXXXXXXXXXXXXTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKYDSKTRTVINAKCMSYTVDESTCGTTP 1572
            CAIIDGLVSQYLINDRQITIDGKLDSRVSLVRNSGGPGLELSSNNRPSFKIDFTENGIREKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCFEKKTEGTSTLTESTTTKEKVTTKSSSKSTTISTSTLPPITTTAPCKLVNGMVSNVFIADHQISINSKSDKNNKNNNINNAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPF HQLIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLISVEIVIEKEEVPSKRLVDLEVIGCFKNITGCFLDGKYYQNHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVNENLECCNCLPNKPNTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKYDSKTRTVINAKCMSYTVDESTCGTTP
Sbjct: 5637 CAIIDGLVSQYLINDRQITIDGKLDSRVSLVRNSGGPGLELSSNNRPSFKIDFTENGIREKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCFEKKTEGTSTLTESTTTKEKVTTKSSSKSTTISTSTLPPITTTAPCKLVNGMVSNVFIADHQISINSKSDKNNKNNNINNAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFTHQLIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLISVEIVIEKEEVPSKRLVDLEVIGCFKNITGCFLDGKYYQNHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVNENLECCNCLPNKPNTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKYDSKTRTVINAKCMSYTVDESTCGTTP 6105          

HSP 2 Score: 750.355 bits (1936), Expect = 0.000e+0
Identity = 487/496 (98.19%), Postives = 491/496 (98.99%), Query Frame = 1
Query:  166 CAIIDGLVSQYLINDRQITIDGKLDSRVSLVRNSGGPGLELSSNNRPSFKIDFTENGIREKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAPCKLVNGMVSNVFIADHQXXXXXXXXXXXXXXXXXXAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQLIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLXXXXXXXXXXXXPSKRLVDLEVIGCFKNITGCFLDGKYYQNHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVXXXXXXXXXXXXXXXTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKYDSKTRTVINAKCMSYTVDESTCGTTPXXXXXXXXXXXXXXXXXXXXXXSVTSA 1653
            CAIIDGLVSQYLINDRQITIDGKLDSRVSLVRNSGGPGLELSSNNRPSFK+DFT+NGIREKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCFEKKTEGTSTLTESTTTKEKVTTKSSSKSTTISTSTLPPITTTAPCKLVNGMVSNVFIADHQISINSKSDKNN  NN   AYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQLIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLISVEIVIE+EEVPSKRLVDLEVIGCFKNITGCFLDGKYYQNHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVNENLECCNCLPNKPNTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKYDSKTRTVINAKCMSYTVDESTCGTTPKESTSSRITTTISKTPIKTETPSVT+A
Sbjct: 2031 CAIIDGLVSQYLINDRQITIDGKLDSRVSLVRNSGGPGLELSSNNRPSFKMDFTDNGIREKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCFEKKTEGTSTLTESTTTKEKVTTKSSSKSTTISTSTLPPITTTAPCKLVNGMVSNVFIADHQISINSKSDKNNNINN---AYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQLIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLISVEIVIEREEVPSKRLVDLEVIGCFKNITGCFLDGKYYQNHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVNENLECCNCLPNKPNTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKYDSKTRTVINAKCMSYTVDESTCGTTPKESTSSRITTTISKTPIKTETPSVTTA 2523          

HSP 3 Score: 744.191 bits (1920), Expect = 0.000e+0
Identity = 486/497 (97.79%), Postives = 489/497 (98.39%), Query Frame = 1
Query:  166 CAIIDGLVSQYLINDRQITIDGKLDSRVSLVRNSGGPGLELSSNNRPSFKIDFTENGIREKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAPCKLVNGMVSNVFIADHQXXXXXXXXXXXXXXXXXXAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQLIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLXXXXXXXXXXXXPSKRLVDLEVIGCFKNITGCFLDGKYYQNHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVXXXXXXXXXXXXXXXTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKYDSKTRTVINAKCMSYTVDESTCGTTPXXXXXXXXXXXXXXXXXXXXXXSVTSAR 1656
            CAIIDGLVSQYLINDRQITIDGK+DSRVSLVRNSGGPGLELSSNN PSFKIDFT+NGI EKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCFEKKTEGTSTLTESTTTKEKVTTKSSSKSTTISTSTLPPITTTAPC LVNGMVSNVFIADHQISINSKSDKNN  NN   AYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQLIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLISVEIVIEKEEVPSKRLVDLEVIGCFKNITGCFLDGKYYQNHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVNENLECCNCLPNKPNTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKY+SKTRTVINAKCMSYTVDESTCGTTPKESTSSRITTTISKTPIKTETPSVTSAR
Sbjct: 4645 CAIIDGLVSQYLINDRQITIDGKVDSRVSLVRNSGGPGLELSSNNLPSFKIDFTDNGIHEKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCFEKKTEGTSTLTESTTTKEKVTTKSSSKSTTISTSTLPPITTTAPCTLVNGMVSNVFIADHQISINSKSDKNNNINN---AYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQLIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLISVEIVIEKEEVPSKRLVDLEVIGCFKNITGCFLDGKYYQNHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVNENLECCNCLPNKPNTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKYNSKTRTVINAKCMSYTVDESTCGTTPKESTSSRITTTISKTPIKTETPSVTSAR 5138          

HSP 4 Score: 499.204 bits (1284), Expect = 4.924e-157
Identity = 323/344 (93.90%), Postives = 324/344 (94.19%), Query Frame = 1
Query:  679 APCKLVNGMVSNVFIADHQXXXXXXXXXXXXXXXXXXAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQ------------------LIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLXXXXXXXXXXXXPSKRLVDLEVIGCFKNITGCFLDGKYYQNHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVXXXXXXXXXXXXXXXTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKYDSKTRTVINAKCMSYTVDESTCGTTPXXXXXXXXXXXXXXXXXXXXXXSVTSAR 1656
            APCKLVNGMVSNVFIADHQISINSKSDKNNKNNNINNAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQ                  LIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLISVEIVIEKEEVPSKRLVDLEVIGCFKNITGCFL GKYYQNHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVNENLECCNCLPNKPNTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKY+SKTRTVIN KCMSYTVDESTCGTTPKESTSSRITTTISKTPIKTETPSVTSAR
Sbjct: 1461 APCKLVNGMVSNVFIADHQISINSKSDKNNKNNNINNAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQVEMFLSIIVIINECFGIQLIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLISVEIVIEKEEVPSKRLVDLEVIGCFKNITGCFLHGKYYQNHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVNENLECCNCLPNKPNTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKYNSKTRTVINTKCMSYTVDESTCGTTPKESTSSRITTTISKTPIKTETPSVTSAR 1804          

HSP 5 Score: 205.682 bits (522), Expect = 4.345e-56
Identity = 125/287 (43.55%), Postives = 180/287 (62.72%), Query Frame = 1
Query:  679 APCKLVNGMVSNVFIADHQXXXXXXXXXXXXXXXXXXAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQLIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLXXXXXXXXXXXXPSKRL-VDLEVIGCFKNITGCFLDGKYYQNHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVXXXXXXXXXXXXXXXTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKYDSKTRTVINAKCMS 1536
             PCK++NG+VS  +I D Q+ IN+K D             LR+SDSSGL I+   S    ID T+NGNREIPFI ++I  +Q+   E    D+    +++          +++ + L S    +        R+ + ++++GCFKN+TGC ++G+ Y+NHQ +N+++   +C++ +C+CSDHK+SCR+    + C KD IK CSKV+ N+ECCNC+ NK  TYCQ+DYLCNPHCG+CL+E D WGK     +   CVCK GYQSFI +YEE+  SKT   +N KCMS
Sbjct: 6210 TPCKVINGLVSEFYIVDQQVKINNKYDSRVSL--------LRESDSSGLIISISSSLVFSIDFTENGNREIPFIEKIIFSEQIGKIEFTAIDKTNTVTKQTKTFTTFPPVLELNKPLKSFIFQVTDSLDGRDRVAIKIQILGCFKNVTGCMVNGRQYENHQLVNSSINSGQCATDMCFCSDHKVSCRSSKLPEKCPKDYIKSCSKVSGNVECCNCVQNKTETYCQVDYLCNPHCGECLNERDSWGKEIATDHPENCVCKDGYQSFIQSYEERAGSKTIYHVNVKCMS 6488          

HSP 6 Score: 155.992 bits (393), Expect = 2.038e-39
Identity = 73/129 (56.59%), Postives = 97/129 (75.19%), Query Frame = 1
Query:  166 CAIIDGLVSQYLINDRQITIDGKLDSRVSLVRNSGGPGLELSSNNRPSFKIDFTENGIREKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCF 552
            CAIIDGLVSQ+LIND QI ++GK D RVS +R S   G E+S  + P+F +DFT+NG+RE  FIYQ+I TGGV+T  +  D +++  ++K+EP V  IN+NIKTL   ++SF  ++EKIFLKIQILGCF
Sbjct: 3773 CAIIDGLVSQFLINDHQIMVNGKPDPRVSFLRFSNEIGFEISLVSLPTFTVDFTDNGLRETMFIYQIILTGGVKTANILTDGVQNMTTFKEEPAVFQINKNIKTLGLELMSFLTSKEKIFLKIQILGCF 3901          

HSP 7 Score: 85.8853 bits (211), Expect = 5.697e-17
Identity = 56/134 (41.79%), Postives = 85/134 (63.43%), Query Frame = 1
Query:  166 CAIIDGLVSQYLINDRQITIDGKLDSRVSLVRNSGGPGLELSSNNRPSFKIDFTENGIREKPFIYQVIFT---GGVETIRVRKDN--LESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCF 552
            C +I+GLVS++ I D+Q+ I+ K DSRVSL+R S   GL +S ++   F IDFTENG RE PFI ++IF+   G +E   + K N   +   ++   P VL +N+ +K+  F V    + ++++ +KIQILGCF
Sbjct: 6212 CKVINGLVSEFYIVDQQVKINNKYDSRVSLLRESDSSGLIISISSSLVFSIDFTENGNREIPFIEKIIFSEQIGKIEFTAIDKTNTVTKQTKTFTTFPPVLELNKPLKSFIFQVTDSLDGRDRVAIKIQILGCF 6345          

HSP 8 Score: 51.2174 bits (121), Expect = 2.409e-6
Identity = 29/75 (38.67%), Postives = 42/75 (56.00%), Query Frame = 1
Query:  166 CAIIDGLVSQYLINDRQITIDGKLDSRVSL--------VRNSGGPGLELSSNNRPSFKIDFTENGIREKPFIYQV 366
            C +++G+VS   I D QI+I+ K D             +R S   GLE+++    + KID T+NG RE PFI+QV
Sbjct: 1463 CKLVNGMVSNVFIADHQISINSKSDKNNKNNNINNAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQV 1537          

HSP 9 Score: 49.6766 bits (117), Expect = 9.002e-6
Identity = 30/78 (38.46%), Postives = 44/78 (56.41%), Query Frame = 1
Query:  679 APCKLVNGMVSNVFIADHQXXXXXXXXXXXXXXXXXXAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQLI 912
             PC +++G+VS   I D QI+I+ K D             +R S   GLE+++    + K+D TDNG RE PFI+Q+I
Sbjct: 2029 PPCAIIDGLVSQYLINDRQITIDGKLDSRVSL--------VRNSGGPGLELSSNNRPSFKMDFTDNGIREKPFIYQVI 2098          
BLAST of SMED30019541 vs. Planmine SMEST
Match: SMESG000025545.1 (SMESG000025545.1)

HSP 1 Score: 754.977 bits (1948), Expect = 0.000e+0
Identity = 468/469 (99.79%), Postives = 468/469 (99.79%), Query Frame = 1
Query:  166 CAIIDGLVSQYLINDRQITIDGKLDSRVSLVRNSGGPGLELSSNNRPSFKIDFTENGIREKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAPCKLVNGMVSNVFIADHQXXXXXXXXXXXXXXXXXXAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQLIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLXXXXXXXXXXXXPSKRLVDLEVIGCFKNITGCFLDGKYYQNHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVXXXXXXXXXXXXXXXTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKYDSKTRTVINAKCMSYTVDESTCGTTP 1572
            CAIIDGLVSQYLINDRQITIDGKLDSRVSLVRNSGGPGLELSSNNRPSFKIDFTENGIREKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCFEKKTEGTSTLTESTTTKEKVTTKSSSKSTTISTSTLPPITTTAPCKLVNGMVSNVFIADHQISINSKSDKNNKNNNINNAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPF HQLIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLISVEIVIEKEEVPSKRLVDLEVIGCFKNITGCFLDGKYYQNHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVNENLECCNCLPNKPNTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKYDSKTRTVINAKCMSYTVDESTCGTTP
Sbjct: 5600 CAIIDGLVSQYLINDRQITIDGKLDSRVSLVRNSGGPGLELSSNNRPSFKIDFTENGIREKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCFEKKTEGTSTLTESTTTKEKVTTKSSSKSTTISTSTLPPITTTAPCKLVNGMVSNVFIADHQISINSKSDKNNKNNNINNAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFTHQLIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLISVEIVIEKEEVPSKRLVDLEVIGCFKNITGCFLDGKYYQNHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVNENLECCNCLPNKPNTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKYDSKTRTVINAKCMSYTVDESTCGTTP 6068          

HSP 2 Score: 744.191 bits (1920), Expect = 0.000e+0
Identity = 486/497 (97.79%), Postives = 489/497 (98.39%), Query Frame = 1
Query:  166 CAIIDGLVSQYLINDRQITIDGKLDSRVSLVRNSGGPGLELSSNNRPSFKIDFTENGIREKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAPCKLVNGMVSNVFIADHQXXXXXXXXXXXXXXXXXXAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQLIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLXXXXXXXXXXXXPSKRLVDLEVIGCFKNITGCFLDGKYYQNHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVXXXXXXXXXXXXXXXTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKYDSKTRTVINAKCMSYTVDESTCGTTPXXXXXXXXXXXXXXXXXXXXXXSVTSAR 1656
            CAIIDGLVSQYLINDRQITIDGK+DSRVSLVRNSGGPGLELSSNN PSFKIDFT+NGI EKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCFEKKTEGTSTLTESTTTKEKVTTKSSSKSTTISTSTLPPITTTAPC LVNGMVSNVFIADHQISINSKSDKNN  NN   AYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQLIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLISVEIVIEKEEVPSKRLVDLEVIGCFKNITGCFLDGKYYQNHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVNENLECCNCLPNKPNTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKY+SKTRTVINAKCMSYTVDESTCGTTPKESTSSRITTTISKTPIKTETPSVTSAR
Sbjct: 4608 CAIIDGLVSQYLINDRQITIDGKVDSRVSLVRNSGGPGLELSSNNLPSFKIDFTDNGIHEKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCFEKKTEGTSTLTESTTTKEKVTTKSSSKSTTISTSTLPPITTTAPCTLVNGMVSNVFIADHQISINSKSDKNNNINN---AYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQLIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLISVEIVIEKEEVPSKRLVDLEVIGCFKNITGCFLDGKYYQNHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVNENLECCNCLPNKPNTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKYNSKTRTVINAKCMSYTVDESTCGTTPKESTSSRITTTISKTPIKTETPSVTSAR 5101          

HSP 3 Score: 649.432 bits (1674), Expect = 0.000e+0
Identity = 418/469 (89.13%), Postives = 424/469 (90.41%), Query Frame = 1
Query:  166 CAIIDGLVSQYLINDRQITIDGKLDSRVSLVRNSGGPGLELSSNNRPSFKIDFTENGIREKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAPCKLVNGMVSNVFIADHQXXXXXXXXXXXXXXXXXXAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQLIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLXXXXXXXXXXXXPSKRLVDLEVIGCFKNITGCFLDGKYYQNHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVXXXXXXXXXXXXXXXTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKYDSKTRTVINAKCMSYTVDESTCGTTP 1572
            CAIIDGLVSQYLINDRQITIDGKLDSRVSLVRNSGGPGLELSSNNRPSFK+DFT+NGIREKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCFEKKTEGTSTLTESTTTKEKVTTKSSSKSTTISTSTLPPITTTAPCKLVNGMVSNVFIADHQ    S + K++KNNNINNAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQLIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLISVEIVIE+EEVPSKRLVDLEVIGCFKNITGCFLDGKYYQ                                       DEIKVCSKVNENLECCNCLPNKPNTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKYDSKTRTVINAKCMSYTVDESTCGTTP
Sbjct: 2033 CAIIDGLVSQYLINDRQITIDGKLDSRVSLVRNSGGPGLELSSNNRPSFKMDFTDNGIREKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCFEKKTEGTSTLTESTTTKEKVTTKSSSKSTTISTSTLPPITTTAPCKLVNGMVSNVFIADHQ---ISINSKSDKNNNINNAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQLIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLISVEIVIEREEVPSKRLVDLEVIGCFKNITGCFLDGKYYQ---------------------------------------DEIKVCSKVNENLECCNCLPNKPNTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKYDSKTRTVINAKCMSYTVDESTCGTTP 2459          

HSP 4 Score: 499.204 bits (1284), Expect = 4.828e-157
Identity = 323/344 (93.90%), Postives = 324/344 (94.19%), Query Frame = 1
Query:  679 APCKLVNGMVSNVFIADHQXXXXXXXXXXXXXXXXXXAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQ------------------LIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLXXXXXXXXXXXXPSKRLVDLEVIGCFKNITGCFLDGKYYQNHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVXXXXXXXXXXXXXXXTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKYDSKTRTVINAKCMSYTVDESTCGTTPXXXXXXXXXXXXXXXXXXXXXXSVTSAR 1656
            APCKLVNGMVSNVFIADHQISINSKSDKNNKNNNINNAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQ                  LIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLISVEIVIEKEEVPSKRLVDLEVIGCFKNITGCFL GKYYQNHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVNENLECCNCLPNKPNTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKY+SKTRTVIN KCMSYTVDESTCGTTPKESTSSRITTTISKTPIKTETPSVTSAR
Sbjct: 1463 APCKLVNGMVSNVFIADHQISINSKSDKNNKNNNINNAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQVEMFLSIIVIINECFGIQLIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLISVEIVIEKEEVPSKRLVDLEVIGCFKNITGCFLHGKYYQNHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVNENLECCNCLPNKPNTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKYNSKTRTVINTKCMSYTVDESTCGTTPKESTSSRITTTISKTPIKTETPSVTSAR 1806          

HSP 5 Score: 205.682 bits (522), Expect = 4.184e-56
Identity = 125/287 (43.55%), Postives = 180/287 (62.72%), Query Frame = 1
Query:  679 APCKLVNGMVSNVFIADHQXXXXXXXXXXXXXXXXXXAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQLIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLXXXXXXXXXXXXPSKRL-VDLEVIGCFKNITGCFLDGKYYQNHQTLNTTMTKEECSSKICYCSDHKISCRTQNPTKTCGKDEIKVCSKVXXXXXXXXXXXXXXXTYCQIDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKYDSKTRTVINAKCMS 1536
             PCK++NG+VS  +I D Q+ IN+K D             LR+SDSSGL I+   S    ID T+NGNREIPFI ++I  +Q+   E    D+    +++          +++ + L S    +        R+ + ++++GCFKN+TGC ++G+ Y+NHQ +N+++   +C++ +C+CSDHK+SCR+    + C KD IK CSKV+ N+ECCNC+ NK  TYCQ+DYLCNPHCG+CL+E D WGK     +   CVCK GYQSFI +YEE+  SKT   +N KCMS
Sbjct: 6173 TPCKVINGLVSEFYIVDQQVKINNKYDSRVSL--------LRESDSSGLIISISSSLVFSIDFTENGNREIPFIEKIIFSEQIGKIEFTAIDKTNTVTKQTKTFTTFPPVLELNKPLKSFIFQVTDSLDGRDRVAIKIQILGCFKNVTGCMVNGRQYENHQLVNSSINSGQCATDMCFCSDHKVSCRSSKLPEKCPKDYIKSCSKVSGNVECCNCVQNKTETYCQVDYLCNPHCGECLNERDSWGKEIATDHPENCVCKDGYQSFIQSYEERAGSKTIYHVNVKCMS 6451          

HSP 6 Score: 155.606 bits (392), Expect = 2.192e-39
Identity = 73/129 (56.59%), Postives = 97/129 (75.19%), Query Frame = 1
Query:  166 CAIIDGLVSQYLINDRQITIDGKLDSRVSLVRNSGGPGLELSSNNRPSFKIDFTENGIREKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCF 552
            CAIIDGLVSQ+LIND QI ++GK D RVS +R S   G E+S  + P+F +DFT+NG+RE  FIYQ+I TGGV+T  +  D +++  ++K+EP V  IN+NIKTL   ++SF  ++EKIFLKIQILGCF
Sbjct: 3736 CAIIDGLVSQFLINDHQIMVNGKPDPRVSFLRFSNEIGFEISLVSLPTFTVDFTDNGLRETMFIYQIILTGGVKTANILTDGVQNMTTFKEEPAVFQINKNIKTLGLELMSFLTSKEKIFLKIQILGCF 3864          

HSP 7 Score: 85.8853 bits (211), Expect = 5.998e-17
Identity = 56/134 (41.79%), Postives = 85/134 (63.43%), Query Frame = 1
Query:  166 CAIIDGLVSQYLINDRQITIDGKLDSRVSLVRNSGGPGLELSSNNRPSFKIDFTENGIREKPFIYQVIFT---GGVETIRVRKDN--LESEVSYKDEPVVLLINENIKTLEFTVLSFKNNQEKIFLKIQILGCF 552
            C +I+GLVS++ I D+Q+ I+ K DSRVSL+R S   GL +S ++   F IDFTENG RE PFI ++IF+   G +E   + K N   +   ++   P VL +N+ +K+  F V    + ++++ +KIQILGCF
Sbjct: 6175 CKVINGLVSEFYIVDQQVKINNKYDSRVSLLRESDSSGLIISISSSLVFSIDFTENGNREIPFIEKIIFSEQIGKIEFTAIDKTNTVTKQTKTFTTFPPVLELNKPLKSFIFQVTDSLDGRDRVAIKIQILGCF 6308          

HSP 8 Score: 51.2174 bits (121), Expect = 2.409e-6
Identity = 29/75 (38.67%), Postives = 42/75 (56.00%), Query Frame = 1
Query:  166 CAIIDGLVSQYLINDRQITIDGKLDSRVSL--------VRNSGGPGLELSSNNRPSFKIDFTENGIREKPFIYQV 366
            C +++G+VS   I D QI+I+ K D             +R S   GLE+++    + KID T+NG RE PFI+QV
Sbjct: 1465 CKLVNGMVSNVFIADHQISINSKSDKNNKNNNINNAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQV 1539          

HSP 9 Score: 49.6766 bits (117), Expect = 8.925e-6
Identity = 30/78 (38.46%), Postives = 44/78 (56.41%), Query Frame = 1
Query:  679 APCKLVNGMVSNVFIADHQXXXXXXXXXXXXXXXXXXAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFIHQLI 912
             PC +++G+VS   I D QI+I+ K D             +R S   GLE+++    + K+D TDNG RE PFI+Q+I
Sbjct: 2031 PPCAIIDGLVSQYLINDRQITIDGKLDSRVSL--------VRNSGGPGLELSSNNRPSFKMDFTDNGIREKPFIYQVI 2100          
The following BLAST results are available for this feature:
BLAST of SMED30019541 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019541 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019541 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019541 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019541 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019541 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019541 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019541 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019541 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019541 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019541 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019541 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019541 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019541 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000025545.14.783e-15799.79SMESG000025545.1[more]
SMESG000025545.14.924e-15799.79SMESG000025545.1[more]
SMESG000025545.14.783e-15799.79SMESG000025545.1[more]
SMESG000025545.14.924e-15799.79SMESG000025545.1[more]
SMESG000025545.14.828e-15799.79SMESG000025545.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30019541 ID=SMED30019541|Name=SMED30019541|organism=Schmidtea mediterranea sexual|type=transcript|length=1761bp
ACATCTTCTGGGACAACAACAACTCCATTAAGTGTATCCACAACTACTCT
TCCGCCATCAGTTTCTACAACGACTATATCTACCAGTAGTCCAACTACTA
AATCAACACCAACTGCTTCATCAACAAGACCTACAACTACTACTCAGCAA
CGCTCTACTCCACCTTGTGCTATTATAGATGGATTAGTTTCACAATATCT
TATAAATGATCGTCAAATAACTATTGACGGGAAACTTGACAGCCGAGTTT
CGCTTGTTCGGAATTCAGGTGGACCCGGATTAGAACTCTCTTCAAATAAT
CGACCAAGTTTCAAAATAGATTTCACTGAAAATGGGATCCGTGAGAAACC
TTTTATTTATCAGGTCATCTTTACTGGAGGAGTTGAAACAATTAGAGTAC
GAAAAGATAATCTTGAATCTGAAGTTAGTTATAAAGACGAACCGGTAGTC
TTGTTAATAAATGAGAATATAAAAACTTTGGAATTTACTGTACTGAGTTT
CAAAAATAATCAAGAAAAAATCTTCCTTAAAATTCAAATCTTGGGATGCT
TCGAGAAAAAGACAGAAGGAACATCAACATTAACAGAATCGACAACAACA
AAAGAGAAAGTAACGACAAAATCTTCCTCGAAATCAACAACGATTTCTAC
ATCGACATTGCCTCCCATAACTACAACGGCTCCATGCAAATTGGTTAATG
GAATGGTATCAAACGTGTTCATTGCTGACCATCAAATCTCTATCAACAGC
AAATCTGATAAGAATAATAAGAATAATAATATTAATAATGCATATAAATT
GCGAAAATCTGACTCGTCTGGATTGGAAATAACTACAGAAGAGAGCACAG
CAATCAAGATTGATCTAACCGACAATGGAAATAGAGAAATTCCATTTATT
CATCAACTGATAATTGGAAAGCAACTTCATTCATTTGAGTTGATCTTTAC
AGATAGAAATAGAATTTCGTCGAGAAAGGTTTATCAATCGGACGAAAAGG
AGTTTACAATTGATGTCGGTGAGTATCTGATTTCCGTGGAAATTGTCATC
GAAAAGGAAGAAGTACCTAGCAAGAGATTAGTAGATTTAGAAGTGATTGG
TTGCTTCAAGAATATAACCGGGTGCTTTTTGGACGGGAAATATTATCAGA
ATCATCAAACCTTGAACACAACAATGACTAAAGAGGAATGCTCATCCAAA
ATCTGCTACTGTTCTGATCATAAAATCAGTTGCCGAACTCAGAATCCAAC
AAAAACGTGCGGAAAGGATGAGATAAAGGTTTGTTCGAAAGTAAATGAAA
ATCTCGAATGCTGCAATTGTTTACCAAATAAGCCAAACACATATTGCCAG
ATTGATTATCTCTGTAATCCTCATTGTGGACAATGTCTTGATGAAAGTGA
CATTTGGGGCAAGGTAGAAATCGTCAAGAATGCAACGGAATGTGTTTGTA
AAAGCGGTTATCAGTCATTCATTGATACATATGAAGAGAAATATGACTCG
AAAACAAGGACAGTCATAAATGCGAAATGCATGTCATACACGGTTGATGA
ATCCACATGTGGAACCACACCGAAAGAAAGTACGAGTTCTAGAATAACGA
CAACAATTTCGAAAACACCAATCAAGACAGAAACTCCAAGTGTGACATCA
GCAAGAAAAAGTACAACGTCAGTCACAAGCCCAACCACTAGTCCAACTAC
TAAATCATCATCCACTGGATCATCAACAACAACTACTGAACCGACAACAA
CAAAAGAACCA
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protein sequence of SMED30019541-orf-1

>SMED30019541-orf-1 ID=SMED30019541-orf-1|Name=SMED30019541-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=587bp
TSSGTTTTPLSVSTTTLPPSVSTTTISTSSPTTKSTPTASSTRPTTTTQQ
RSTPPCAIIDGLVSQYLINDRQITIDGKLDSRVSLVRNSGGPGLELSSNN
RPSFKIDFTENGIREKPFIYQVIFTGGVETIRVRKDNLESEVSYKDEPVV
LLINENIKTLEFTVLSFKNNQEKIFLKIQILGCFEKKTEGTSTLTESTTT
KEKVTTKSSSKSTTISTSTLPPITTTAPCKLVNGMVSNVFIADHQISINS
KSDKNNKNNNINNAYKLRKSDSSGLEITTEESTAIKIDLTDNGNREIPFI
HQLIIGKQLHSFELIFTDRNRISSRKVYQSDEKEFTIDVGEYLISVEIVI
EKEEVPSKRLVDLEVIGCFKNITGCFLDGKYYQNHQTLNTTMTKEECSSK
ICYCSDHKISCRTQNPTKTCGKDEIKVCSKVNENLECCNCLPNKPNTYCQ
IDYLCNPHCGQCLDESDIWGKVEIVKNATECVCKSGYQSFIDTYEEKYDS
KTRTVINAKCMSYTVDESTCGTTPKESTSSRITTTISKTPIKTETPSVTS
ARKSTTSVTSPTTSPTTKSSSTGSSTTTTEPTTTKEP
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000028Category 2 cell
PLANA:0000420parapharyngeal region
PLANA:0003116parenchymal cell
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..54