SMED30019536

Overview
NameSMED30019536
Smed IDSMED30019536
Length (bp)668
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30019536 (SMED30019536) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30019536 (SMED30019536) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30019536 (SMED30019536) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30019536 (SMED30019536) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30019536

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X2 cellSMED30019536 SmedASXL_077253SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of SMED30019536 vs. Planmine SMEST
Match: SMESG000063074.1 (SMESG000063074.1)

HSP 1 Score: 222.246 bits (565), Expect = 2.114e-74
Identity = 106/135 (78.52%), Postives = 115/135 (85.19%), Query Frame = 2
Query:  212 MPLDWTAIQAPDEQCRKRTQQGTEIFDELRQRWPYPTTWRLLGFEKEPCGLTGQQYPAAETYEHARNLLTQWERVQEQVDLNVVKARMYLANVEEDIRVGRIAEAYPPRPARDTRRPYPVAAQRKSLKRHFTEES 616
            M LDWT  QA  E+  ++  Q  +I D+LR +W YPTTWRLLGFE +PCGL GQQYPA ETYEHARNLLT WERVQEQVDLNV+KARMYLANVEEDIRVGRIAEAYPPRPARDTRRPYPVAA RKSLKRH TE+S
Sbjct:   13 MSLDWTTTQATSEKHPRKAPQAADIIDKLRPQWSYPTTWRLLGFETDPCGLAGQQYPAEETYEHARNLLTHWERVQEQVDLNVIKARMYLANVEEDIRVGRIAEAYPPRPARDTRRPYPVAALRKSLKRHSTEKS 147          
BLAST of SMED30019536 vs. Planmine SMEST
Match: SMESG000073411.1 (SMESG000073411.1)

HSP 1 Score: 221.861 bits (564), Expect = 4.556e-73
Identity = 104/140 (74.29%), Postives = 114/140 (81.43%), Query Frame = 2
Query:  194 HSNKKDMPLDWTAIQAPDEQCRKRTQQGTEIFDELRQRWPYPTTWRLLGFEKEPCGLTGQQYPAAETYEHARNLLTQWERVQEQVDLNVVKARMYLANVEEDIRVGRIAEAYPPRPARDTRRPYPVAAQRKSLKRHFTEE 613
            H +  D+PLDWTA Q  D+Q R+     T IFD+LRQ+WPYPTTWR+LGFEK+PCGL GQQYPAAE Y HARNLLTQWERVQEQVDL VVKARMYLANVEEDI  GRIAEAYPPRP RDTRRPYPV   +K+LKR F  E
Sbjct:   90 HDDILDIPLDWTASQVTDKQGREGAPSTTAIFDKLRQQWPYPTTWRILGFEKDPCGLAGQQYPAAEAYAHARNLLTQWERVQEQVDLQVVKARMYLANVEEDILAGRIAEAYPPRPVRDTRRPYPVVTPKKNLKRPFNGE 229          
BLAST of SMED30019536 vs. Planmine SMEST
Match: SMESG000005029.1 (SMESG000005029.1)

HSP 1 Score: 214.927 bits (546), Expect = 1.268e-70
Identity = 100/140 (71.43%), Postives = 114/140 (81.43%), Query Frame = 2
Query:  194 HSNKKDMPLDWTAIQAPDEQCRKRTQQGTEIFDELRQRWPYPTTWRLLGFEKEPCGLTGQQYPAAETYEHARNLLTQWERVQEQVDLNVVKARMYLANVEEDIRVGRIAEAYPPRPARDTRRPYPVAAQRKSLKRHFTEE 613
              ++K+MPL+WT  Q PDE+CR+ +    EIF E+RQ+WPYPTTWRLLG   +PCGL GQQYPAAETY +ARNLLTQWERVQEQVDL VV ARMYLANVEEDI  GRIAEAYPPRP RDTRRPYPV   +K+LKR F+ E
Sbjct:   70 QDDEKNMPLEWTDTQVPDERCRQESPPKAEIFKEIRQQWPYPTTWRLLGLNSDPCGLAGQQYPAAETYTYARNLLTQWERVQEQVDLQVVNARMYLANVEEDILAGRIAEAYPPRPVRDTRRPYPVVPPKKNLKRPFSGE 209          
BLAST of SMED30019536 vs. Planmine SMEST
Match: SMESG000012865.1 (SMESG000012865.1)

HSP 1 Score: 206.068 bits (523), Expect = 3.664e-68
Identity = 98/134 (73.13%), Postives = 109/134 (81.34%), Query Frame = 2
Query:  212 MPLDWTAIQAPDEQCRKRTQQGTEIFDELRQRWPYPTTWRLLGFEKEPCGLTGQQYPAAETYEHARNLLTQWERVQEQVDLNVVKARMYLANVEEDIRVGRIAEAYPPRPARDTRRPYPVAAQRKSLKRHFTEE 613
            MPL+WT  Q PDE+CRK +    EIF E+RQ+WPYPTTWRLLG   +PCGL  QQYPAAETY +ARNLLTQWERVQEQVDL VVKARMYLAN+EEDI  GRIAEAYPPRP RDTRRPYPV   +++LKR F  E
Sbjct:    1 MPLEWTDTQVPDERCRKESPPKAEIFKEIRQQWPYPTTWRLLGLNSDPCGLARQQYPAAETYTYARNLLTQWERVQEQVDLQVVKARMYLANLEEDILAGRIAEAYPPRPVRDTRRPYPVVPPKRNLKRPFNGE 134          
BLAST of SMED30019536 vs. Planmine SMEST
Match: SMESG000066545.1 (SMESG000066545.1)

HSP 1 Score: 213.386 bits (542), Expect = 5.182e-67
Identity = 99/134 (73.88%), Postives = 110/134 (82.09%), Query Frame = 2
Query:  203 KKDMPLDWTAIQAPDEQCRKRTQQGTEIFDELRQRWPYPTTWRLLGFEKEPCGLTGQQYPAAETYEHARNLLTQWERVQEQVDLNVVKARMYLANVEEDIRVGRIAEAYPPRPARDTRRPYPVAAQRKSLKRHF 604
            +K+MPL+WT  Q PDEQCRK +    EIF E+RQ+WPYPTTWRLLG  ++PCGL GQQYPAAETY  ARNLLTQWERVQEQVDL VVKARMYLANVEEDI  GR+AEAYPPRP  DTR PYPV   +KSLK+ F
Sbjct:   30 EKNMPLEWTDTQVPDEQCRKESPPKAEIFKEIRQQWPYPTTWRLLGLNRDPCGLAGQQYPAAETYTCARNLLTQWERVQEQVDLQVVKARMYLANVEEDILAGRVAEAYPPRPVHDTRLPYPVVPPKKSLKKPF 163          
The following BLAST results are available for this feature:
BLAST of SMED30019536 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019536 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019536 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019536 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019536 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019536 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019536 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019536 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019536 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019536 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019536 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019536 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019536 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019536 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000063074.12.114e-7478.52SMESG000063074.1[more]
SMESG000073411.14.556e-7374.29SMESG000073411.1[more]
SMESG000005029.11.268e-7071.43SMESG000005029.1[more]
SMESG000012865.13.664e-6873.13SMESG000012865.1[more]
SMESG000066545.15.182e-6773.88SMESG000066545.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30019536 ID=SMED30019536|Name=SMED30019536|organism=Schmidtea mediterranea sexual|type=transcript|length=668bp
ATCTCGTTTTCCATTTTAGTATTATGTAACATTAATTTATTCAACAAGGA
AACTTGTCAGATAAAAATAATAGTTGTGAATAATGGTGGTTAAGAAGTCA
GTGATCCGATCGGGAATAGATGGAGGAGATTGAAATGAAACAAAAACTAT
GAAGCCTCAACATATTATCAAATAAGCACATTAAAGAAATTGTCATTCAA
ACAAAAAGGACATGCCGCTCGATTGGACCGCTATTCAAGCACCCGACGAA
CAGTGTCGGAAGAGAACACAGCAAGGTACAGAAATTTTCGATGAACTTCG
ACAACGGTGGCCGTATCCCACCACTTGGCGGCTGCTGGGCTTCGAAAAGG
AACCTTGTGGACTCACCGGACAGCAGTATCCTGCTGCAGAAACATACGAA
CACGCACGGAACCTCCTCACCCAATGGGAAAGGGTGCAGGAACAAGTAGA
CTTAAATGTGGTCAAGGCAAGGATGTACCTCGCGAACGTGGAAGAGGACA
TCCGCGTCGGACGTATTGCGGAGGCTTACCCACCTCGACCGGCCCGCGAT
ACCCGACGGCCCTATCCGGTGGCGGCTCAAAGGAAAAGCCTAAAAAGACA
TTTCACCGAGGAGTCCTGATTCCTCGGGCAGGGGATAAAGACAATAGTTA
CAGTGTTAATGTTATAAA
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002111X2 cell