SMED30019528

Overview
NameSMED30019528
Smed IDSMED30019528
Length (bp)1506
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30019528 (SMED30019528) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30019528 (SMED30019528) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30019528 (SMED30019528) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30019528 (SMED30019528) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30019528

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30019528SMESG000009339.1 SmedASXL_015198SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
cephalic gangliaSMED30019528SMESG000009339.1 dd_Smed_v4_9636_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30019528SMESG000009339.1 dd_Smed_v4_9636_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
zeta neoblastSMED30019528SMESG000009339.1 dd_Smed_v4_9636_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30019528 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.002182:556..4260 -7353
Homology
BLAST of SMED30019528 vs. Ensembl Human
Match: FAM120B (family with sequence similarity 120B [Source:HGNC Symbol;Acc:HGNC:21109])

HSP 1 Score: 56.9954 bits (136), Expect = 6.516e-8
Identity = 54/198 (27.27%), Postives = 86/198 (43.43%), Query Frame = 3
Query:  279 IPGAEYDELLNAFRDFHMMFVNIGVKLIYIADGPKGLDKLNQQQKEKSWIYRFERNMR-VYKTYLDMCKNPPSPLQKIFEKIIRPCLTSDMFKNIIIKEGGTIINLIYGEADYQIANMRRERNSWAVLTNDSDFFIFHNCPTIHIGLFDIDRDFMHGRNVRNPKRLTVGLTSDKILMDRLRLSTHDKLIDFSILMGND 869
            I G ++ E  +A RDF   F   G+KLI+  DG      + +Q K   W+ R  +N R + + +  +  +   P + +F   I   L   +F    +K  G        EADY++A+   + N   +L  D+D+ I+  CP      F I    +   +       TV L  +K L + L L   D L   + L+GND
Sbjct:   71 ICGGQWREYFSALRDFVKTFTAAGIKLIFFFDG------MVEQDKRDEWVKRRLKNNREISRIFHYIKSHKEQPGRNMF--FIPSGLA--VFTRFALKTLGQETLCSLQEADYEVASYGLQHNCLGILGEDTDYLIYDTCP-----YFSISELCLESLD-------TVMLCREK-LCESLGLCVAD-LPLLACLLGND 244          
BLAST of SMED30019528 vs. Ensembl Human
Match: FAM120B (family with sequence similarity 120B [Source:HGNC Symbol;Acc:HGNC:21109])

HSP 1 Score: 56.9954 bits (136), Expect = 6.516e-8
Identity = 54/198 (27.27%), Postives = 86/198 (43.43%), Query Frame = 3
Query:  279 IPGAEYDELLNAFRDFHMMFVNIGVKLIYIADGPKGLDKLNQQQKEKSWIYRFERNMR-VYKTYLDMCKNPPSPLQKIFEKIIRPCLTSDMFKNIIIKEGGTIINLIYGEADYQIANMRRERNSWAVLTNDSDFFIFHNCPTIHIGLFDIDRDFMHGRNVRNPKRLTVGLTSDKILMDRLRLSTHDKLIDFSILMGND 869
            I G ++ E  +A RDF   F   G+KLI+  DG      + +Q K   W+ R  +N R + + +  +  +   P + +F   I   L   +F    +K  G        EADY++A+   + N   +L  D+D+ I+  CP      F I    +   +       TV L  +K L + L L   D L   + L+GND
Sbjct:   71 ICGGQWREYFSALRDFVKTFTAAGIKLIFFFDG------MVEQDKRDEWVKRRLKNNREISRIFHYIKSHKEQPGRNMF--FIPSGLA--VFTRFALKTLGQETLCSLQEADYEVASYGLQHNCLGILGEDTDYLIYDTCP-----YFSISELCLESLD-------TVMLCREK-LCESLGLCVAD-LPLLACLLGND 244          
BLAST of SMED30019528 vs. Ensembl Human
Match: FAM120B (family with sequence similarity 120B [Source:HGNC Symbol;Acc:HGNC:21109])

HSP 1 Score: 56.9954 bits (136), Expect = 6.546e-8
Identity = 54/198 (27.27%), Postives = 86/198 (43.43%), Query Frame = 3
Query:  279 IPGAEYDELLNAFRDFHMMFVNIGVKLIYIADGPKGLDKLNQQQKEKSWIYRFERNMR-VYKTYLDMCKNPPSPLQKIFEKIIRPCLTSDMFKNIIIKEGGTIINLIYGEADYQIANMRRERNSWAVLTNDSDFFIFHNCPTIHIGLFDIDRDFMHGRNVRNPKRLTVGLTSDKILMDRLRLSTHDKLIDFSILMGND 869
            I G ++ E  +A RDF   F   G+KLI+  DG      + +Q K   W+ R  +N R + + +  +  +   P + +F   I   L   +F    +K  G        EADY++A+   + N   +L  D+D+ I+  CP      F I    +   +       TV L  +K L + L L   D L   + L+GND
Sbjct:   59 ICGGQWREYFSALRDFVKTFTAAGIKLIFFFDG------MVEQDKRDEWVKRRLKNNREISRIFHYIKSHKEQPGRNMF--FIPSGLA--VFTRFALKTLGQETLCSLQEADYEVASYGLQHNCLGILGEDTDYLIYDTCP-----YFSISELCLESLD-------TVMLCREK-LCESLGLCVAD-LPLLACLLGND 232          
BLAST of SMED30019528 vs. Ensembl Human
Match: FAM120B (family with sequence similarity 120B [Source:HGNC Symbol;Acc:HGNC:21109])

HSP 1 Score: 56.9954 bits (136), Expect = 6.546e-8
Identity = 54/198 (27.27%), Postives = 86/198 (43.43%), Query Frame = 3
Query:  279 IPGAEYDELLNAFRDFHMMFVNIGVKLIYIADGPKGLDKLNQQQKEKSWIYRFERNMR-VYKTYLDMCKNPPSPLQKIFEKIIRPCLTSDMFKNIIIKEGGTIINLIYGEADYQIANMRRERNSWAVLTNDSDFFIFHNCPTIHIGLFDIDRDFMHGRNVRNPKRLTVGLTSDKILMDRLRLSTHDKLIDFSILMGND 869
            I G ++ E  +A RDF   F   G+KLI+  DG      + +Q K   W+ R  +N R + + +  +  +   P + +F   I   L   +F    +K  G        EADY++A+   + N   +L  D+D+ I+  CP      F I    +   +       TV L  +K L + L L   D L   + L+GND
Sbjct:   59 ICGGQWREYFSALRDFVKTFTAAGIKLIFFFDG------MVEQDKRDEWVKRRLKNNREISRIFHYIKSHKEQPGRNMF--FIPSGLA--VFTRFALKTLGQETLCSLQEADYEVASYGLQHNCLGILGEDTDYLIYDTCP-----YFSISELCLESLD-------TVMLCREK-LCESLGLCVAD-LPLLACLLGND 232          
BLAST of SMED30019528 vs. Ensembl Human
Match: FAM120B (family with sequence similarity 120B [Source:HGNC Symbol;Acc:HGNC:21109])

HSP 1 Score: 56.9954 bits (136), Expect = 6.827e-8
Identity = 54/198 (27.27%), Postives = 86/198 (43.43%), Query Frame = 3
Query:  279 IPGAEYDELLNAFRDFHMMFVNIGVKLIYIADGPKGLDKLNQQQKEKSWIYRFERNMR-VYKTYLDMCKNPPSPLQKIFEKIIRPCLTSDMFKNIIIKEGGTIINLIYGEADYQIANMRRERNSWAVLTNDSDFFIFHNCPTIHIGLFDIDRDFMHGRNVRNPKRLTVGLTSDKILMDRLRLSTHDKLIDFSILMGND 869
            I G ++ E  +A RDF   F   G+KLI+  DG      + +Q K   W+ R  +N R + + +  +  +   P + +F   I   L   +F    +K  G        EADY++A+   + N   +L  D+D+ I+  CP      F I    +   +       TV L  +K L + L L   D L   + L+GND
Sbjct:   82 ICGGQWREYFSALRDFVKTFTAAGIKLIFFFDG------MVEQDKRDEWVKRRLKNNREISRIFHYIKSHKEQPGRNMF--FIPSGLA--VFTRFALKTLGQETLCSLQEADYEVASYGLQHNCLGILGEDTDYLIYDTCP-----YFSISELCLESLD-------TVMLCREK-LCESLGLCVAD-LPLLACLLGND 255          
BLAST of SMED30019528 vs. Ensembl Zebrafish
Match: fam120b (family with sequence similarity 120B [Source:ZFIN;Acc:ZDB-GENE-040801-119])

HSP 1 Score: 56.225 bits (134), Expect = 6.759e-8
Identity = 62/266 (23.31%), Postives = 109/266 (40.98%), Query Frame = 3
Query:  279 IPGAEYDELLNAFRDFHMMFVNIGVKLIYIADGPKGLDKLNQQQKEKSWIYRFER-NMRVYKTYLDMCKNPPSPLQK-IFEKIIRPCLTSDM--FKNIIIKEGGTIINLIYGEADYQIANMRRERNSWAVLTNDSDFFIFHNCPTIHIGLFDIDRDFMHGRNVRNPKRLTVGLTSDKILMDRLRLSTHDKLIDFSILMGNDYTVDFS-ENLRNFCEIKWKYDFSDIPHSMKVAEWVEKQSTKLIDSSEFGSFSIDKPELKLSVQFS 1061
            + G ++ E +N  ++F   F   G++LI+  DG        +++K   WI R  R N  + K +  +  +   P  + +F      CL S +  F    +K  G        E DY++A+     N   +L  D+DF I++  P + I    ++              +T  L S + L   L+L  HD L   S L+GND   +   + LRN     ++    ++            Q  ++  ++EF   +I KP  + +V  S
Sbjct:   59 VHGGQWKEYINILQEFINAFTAAGIRLIFFFDGTV------EERKRSEWIKRRLRVNQDIAKVFQHIKTHNQQPNSRNLF------CLPSGLATFSRFALKSLGQETFCSVREGDYEVADYALSHNCMGILGQDTDFVIYNTVPYLSISKLHLN-------------SMTTVLFSREKLCRVLQLHVHD-LPLLSCLLGNDVVHEQQMQRLRNIALDSYRMKCKEV------------QGDRVYAAAEF--INIHKPSCEGTVGLS 284          
BLAST of SMED30019528 vs. Ensembl Xenopus
Match: fam120b (Xenopus tropicalis family with sequence similarity 120B (fam120b), mRNA. [Source:RefSeq mRNA;Acc:NM_001367998])

HSP 1 Score: 52.7582 bits (125), Expect = 1.154e-6
Identity = 55/212 (25.94%), Postives = 92/212 (43.40%), Query Frame = 3
Query:   93 MGIPGLFTFLKRNGCY---ETYDLVALA----KSNPDLE--ILVDFYGIEQHILERTLLKLISKYQNEYLLIPGAEYDELLNAFRDFHMMFVNIGVKLIYIADGPKGLDKLNQQQKEKSWIYRFER-NMRVYKTYLDMCKNPPSPLQKIFEKIIRPCLTSDMFKNIIIKEGGTIINLIYGEADYQIANMRRERNSWAVLTNDSDFFIFHNCP 698
            MGI GL +F++ N C     T +L  LA     SNP+    I+VD  G             +  +      + G ++ E L + + F   F   G++L++I DG      + ++ K + W+ R  R N  + K +  +  N   P + +F     P   S   K+ +   G  +I L + E DY++A    +     +L  DSD+ IF+  P
Sbjct:    1 MGIKGLQSFVQ-NSCSNVCSTVNLRTLAARHKSSNPESSPTIVVDAMGC------------LRTWYTPNDWVHGGQWKEYLCSLQTFISAFQAAGIRLVFIFDG------VIEKTKREEWVKRRLRDNNEISKIFDFIKANGQQPGRNMF---FIPSGISTFTKSALKSLGQEVI-LSHVEGDYEVAAYGLQHQCLGILGEDSDYLIFNTVP 189          
BLAST of SMED30019528 vs. Ensembl Xenopus
Match: fam120b (Xenopus tropicalis family with sequence similarity 120B (fam120b), mRNA. [Source:RefSeq mRNA;Acc:NM_001367998])

HSP 1 Score: 52.7582 bits (125), Expect = 1.166e-6
Identity = 55/212 (25.94%), Postives = 90/212 (42.45%), Query Frame = 3
Query:   93 MGIPGLFTFLKRNGCY---ETYDLVALA----KSNPDLE--ILVDFYGIEQHILERTLLKLISKYQNEYLLIPGAEYDELLNAFRDFHMMFVNIGVKLIYIADGPKGLDKLNQQQKEKSWIYRFERNMRVYKTYLDMCK-NPPSPLQKIFEKIIRPCLTSDMFKNIIIKEGGTIINLIYGEADYQIANMRRERNSWAVLTNDSDFFIFHNCP 698
            MGI GL +F++ N C     T +L  LA     SNP+    I+VD  G             +  +      + G ++ E L + + F   F   G++L++I DG      + ++ K + W+ R  R+        D  K N   P + +F     P   S   K+ +   G  +I L + E DY++A    +     +L  DSD+ IF+  P
Sbjct:    1 MGIKGLQSFVQ-NSCSNVCSTVNLRTLAARHKSSNPESSPTIVVDAMGC------------LRTWYTPNDWVHGGQWKEYLCSLQTFISAFQAAGIRLVFIFDG------VIEKTKREEWVKRRLRDNNEISKIFDFIKANGQQPGRNMF---FIPSGISTFTKSALKSLGQEVI-LSHVEGDYEVAAYGLQHQCLGILGEDSDYLIFNTVP 189          
BLAST of SMED30019528 vs. Ensembl Mouse
Match: Fam120b (family with sequence similarity 120, member B [Source:MGI Symbol;Acc:MGI:1914794])

HSP 1 Score: 55.4546 bits (132), Expect = 1.166e-7
Identity = 56/212 (26.42%), Postives = 92/212 (43.40%), Query Frame = 3
Query:  279 IPGAEYDELLNAFRDFHMMFVNIGVKLIYIADGPKGLDKLNQQQKEKSWIYRFERNMR-VYKTYLDMCKNPPSPLQKIFEKIIRPCLTSDMFKNIIIKEGGTIINLIYGEADYQIANMRRERNSWAVLTNDSDFFIFHNCPTIHIGLFDIDRDFMHGRNVRNPKRLTVGLTSDKILMDRLRLSTHDKLIDFSILMGNDYTVD-FSENLRNFC 908
            + G ++ E   A R+F   F + G+KLI+  DG      + +  K   W+ R  +N R + K +  +      P + +F   I   L   +F    +K  G        EADY++A+   + N   +L  D+D+ I+  CP   IG  D+  + +           T+ L  +K L + L L   D L   + L+GND T +   E+ R  C
Sbjct:   59 VCGGQWREYYCALRNFVAAFTSAGIKLIFFFDG------MVEPGKRDEWVKRRLKNNREISKIFHYIKSKRDQPGRNMF--FIPSGLA--IFTRFALKTLGQETFCSLQEADYEVASYGLQHNCLGILGEDTDYLIYDTCPYFSIG--DLCLESLQ----------TIMLCREK-LCESLGLRVAD-LPLLACLLGNDITPESMFESFRYKC 246          
BLAST of SMED30019528 vs. UniProt/SwissProt
Match: sp|Q96EK7|F120B_HUMAN (Constitutive coactivator of peroxisome proliferator-activated receptor gamma OS=Homo sapiens OX=9606 GN=FAM120B PE=1 SV=1)

HSP 1 Score: 56.9954 bits (136), Expect = 3.144e-7
Identity = 54/198 (27.27%), Postives = 86/198 (43.43%), Query Frame = 3
Query:  279 IPGAEYDELLNAFRDFHMMFVNIGVKLIYIADGPKGLDKLNQQQKEKSWIYRFERNMR-VYKTYLDMCKNPPSPLQKIFEKIIRPCLTSDMFKNIIIKEGGTIINLIYGEADYQIANMRRERNSWAVLTNDSDFFIFHNCPTIHIGLFDIDRDFMHGRNVRNPKRLTVGLTSDKILMDRLRLSTHDKLIDFSILMGND 869
            I G ++ E  +A RDF   F   G+KLI+  DG      + +Q K   W+ R  +N R + + +  +  +   P + +F   I   L   +F    +K  G        EADY++A+   + N   +L  D+D+ I+  CP      F I    +   +       TV L  +K L + L L   D L   + L+GND
Sbjct:   59 ICGGQWREYFSALRDFVKTFTAAGIKLIFFFDG------MVEQDKRDEWVKRRLKNNREISRIFHYIKSHKEQPGRNMF--FIPSGLA--VFTRFALKTLGQETLCSLQEADYEVASYGLQHNCLGILGEDTDYLIYDTCP-----YFSISELCLESLD-------TVMLCREK-LCESLGLCVAD-LPLLACLLGND 232          
BLAST of SMED30019528 vs. UniProt/SwissProt
Match: sp|A6QNT4|F120B_BOVIN (Constitutive coactivator of peroxisome proliferator-activated receptor gamma OS=Bos taurus OX=9913 GN=FAM120B PE=2 SV=1)

HSP 1 Score: 56.6102 bits (135), Expect = 4.180e-7
Identity = 39/151 (25.83%), Postives = 68/151 (45.03%), Query Frame = 3
Query:  279 IPGAEYDELLNAFRDFHMMFVNIGVKLIYIADGPKGLDKLNQQQKEKSWIYRFERNMR-VYKTYLDMCKNPPSPLQKIFEKIIRPCLTSDMFKNIIIKEGGTIINLIYGEADYQIANMRRERNSWAVLTNDSDFFIFHNCPTIHIGLFDID 728
            + G ++ E  ++ R+F   F  +G+KLI+  DG      + +Q K   W+ R  +N R + K +  +      P + +F   I   L   +F    +K  G        EADY++A+   + N   +L  D+D+ I+  CP   I    +D
Sbjct:   59 VCGGQWREYYSSLREFVRTFTAVGIKLIFFFDG------MVEQSKRDEWVKRRLKNNREIAKIFHYIKSRREQPGRNMF--FIPSGLA--IFTRFALKALGQETLCSLQEADYEVASYGFQNNCLGILGEDTDYLIYDTCPYFSISELSLD 199          
BLAST of SMED30019528 vs. UniProt/SwissProt
Match: sp|Q6RI63|F120B_MOUSE (Constitutive coactivator of peroxisome proliferator-activated receptor gamma OS=Mus musculus OX=10090 GN=Fam120b PE=1 SV=1)

HSP 1 Score: 55.4546 bits (132), Expect = 8.159e-7
Identity = 56/212 (26.42%), Postives = 92/212 (43.40%), Query Frame = 3
Query:  279 IPGAEYDELLNAFRDFHMMFVNIGVKLIYIADGPKGLDKLNQQQKEKSWIYRFERNMR-VYKTYLDMCKNPPSPLQKIFEKIIRPCLTSDMFKNIIIKEGGTIINLIYGEADYQIANMRRERNSWAVLTNDSDFFIFHNCPTIHIGLFDIDRDFMHGRNVRNPKRLTVGLTSDKILMDRLRLSTHDKLIDFSILMGNDYTVD-FSENLRNFC 908
            + G ++ E   A R+F   F + G+KLI+  DG      + +  K   W+ R  +N R + K +  +      P + +F   I   L   +F    +K  G        EADY++A+   + N   +L  D+D+ I+  CP   IG  D+  + +           T+ L  +K L + L L   D L   + L+GND T +   E+ R  C
Sbjct:   59 VCGGQWREYYCALRNFVAAFTSAGIKLIFFFDG------MVEPGKRDEWVKRRLKNNREISKIFHYIKSKRDQPGRNMF--FIPSGLA--IFTRFALKTLGQETFCSLQEADYEVASYGLQHNCLGILGEDTDYLIYDTCPYFSIG--DLCLESLQ----------TIMLCREK-LCESLGLRVAD-LPLLACLLGNDITPESMFESFRYKC 246          
BLAST of SMED30019528 vs. TrEMBL
Match: A0A0L8GFX0 (Uncharacterized protein OS=Octopus bimaculoides OX=37653 GN=OCBIM_22034150mg PE=4 SV=1)

HSP 1 Score: 143.28 bits (360), Expect = 5.334e-33
Identity = 112/411 (27.25%), Postives = 200/411 (48.66%), Query Frame = 3
Query:   93 MGIPGLFTFLKRNG--CYETYDLVALAKSNPDLEILVDFYGIEQHILERTLLKLISKYQNEYLLIPGAEYDELLNAFRDFHMMFVNIGVKLIYIADGPKGLDKLNQQQKEKSWIYRFERNMRVYKTYLDMCKNPPSPLQKIFEKIIRPCLTSDMFKNIIIKEGGTIINLIYGEADYQIANMRRERNSWAVLTNDSDFFIFHNCPTIHIGLFDIDRDF---MHGRNVRNPKRLTVGLTSDKILMDRLRLSTHDKLIDFSILMGNDYTVDFSENLRNFCEIKWKYDFSDIPHSMK-VAEWVEKQSTKLIDSSEFGSFSI-DKPELKLSVQFSMDFYKQIPTEPIPQFTSKDNEEFWNYLVDRYQKGEISSMCLSVFHGFCYQRNCVQPIIVGVNKDINSLLVPLKTRIYSLLL 1304
            MG+ GLF++  +N   C    DLV +A+    +EILVD+Y     + ++ +  L++  +N+YLL+ G EY  L            ++ + L ++ DG +G  K   +++  +W+ R +++MR  K  L +  N  S    + + I+RP L   +F + + + G  ++N   GEAD  +A    +  ++A+ + DSDF +F +C  I   LFDI  D      G   + P  L +GL S + +     LS+ D+LI+ +I+ GND T   S  +RN   + +K D  +  + ++ +  WV  +S K I++       + +  E  L+VQ S  FYK    E +  +   +       +++R +   +    L   HG  +Q+   +  +      I +LL  L++ IY +LL
Sbjct:    1 MGVQGLFSYCLKNQKQCCTFVDLVEVAREQKGIEILVDYYCFANWVYDKFMTNLLNINKNKYLLLYGGEYSSLSLYVTALIKNLQSVDIHLTFLIDGARGSCKETTKRRLNTWLSRSQKDMRKIKNMLSVFYNEISIEDLLCDDIVRPVLRDMIFTSALTECGCKVVN-CSGEADDMMAKQLLDSKAFAIFSYDSDFCLFKDCCCIPPKLFDIHNDMELEYCGEVPKKPLHLQIGLISTERVSHMFGLSS-DELIELAIVCGNDLT---SHYMRNNQTLLYKLDIRERKNQLENIVAWV--KSYKRIENHPLLDNEMRNNSEFALAVQLSRSFYK---LELVQDYDDNNKGSLTELVIERLKTKTLHPKMLGAHHGLYWQKVLFED-LYNYWPGIETLLSNLRSCIYRILL 400          
BLAST of SMED30019528 vs. TrEMBL
Match: A0A210QAR5 (Uncharacterized protein OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT01511 PE=4 SV=1)

HSP 1 Score: 138.272 bits (347), Expect = 2.809e-31
Identity = 109/416 (26.20%), Postives = 181/416 (43.51%), Query Frame = 3
Query:   93 MGIPGLFT--FLKRNGCYETYDLVALAKSNPDLEILVDFYGIEQHILERTLLKLISKYQNEYLLIPGAEYDELLNAFRDFHMMFVNIGVKLIYIADGPKGLDKLNQQQKEKSWIYRFERNMRVYKTYLDMCKN--PPSPLQKIFEKIIRPCLTSDMFKNIIIKEGGTIINLIYGEADYQIANMRRERN-SWAVLTNDSDFFIFHNCPTIHIGLFDIDRDFMHGRNVR---NPKRLTVGLTSDKILMDRLRLSTHDKLIDFSILMGNDYTVDF--SENLRNFCEIKWKYDFSDIPHSMKVAEWVEKQSTKLIDSSEFGSFSIDKPELKLSVQFSMDFYKQIPTEPIPQFTSKDNEEFWNYLVDRYQKGEISSMCLSVFHGFCYQRNCVQPIIVGVNKDINSLLVPLKTRIYSLLLQK 1310
            MG+ GL +    +RN C    DL+ +A+    +EILVD+Y  E  ++E+    L     N+YL I G EY  L    +        +G++L++  DG +G      ++K  +WI R  R +   +  + +C    P + LQ    + +R  L  + FK+ + +    +     GEAD  IA    +R  ++AV +NDSDF IF +C  I    FD+  D   G        P+RL VG+ +   +M  L L  HD L++ SI+ GND+T  +     L+   EIK +           VA+W+   +        F          + +V  S +FY       +P             L    ++G   +  + + + F + R  V+ +  G        L PL+  +Y ++LQ+
Sbjct:    1 MGVRGLLSACMRQRNACVHEVDLIQVARERGGIEILVDYYSFELFVMEQFWFSLCHHQHNDYLWILGGEYASLDVYLKKLIQHLKALGIQLVFFVDGARGASTETTREKLDTWIERHHRELGKIRDIMSVCCGFMPITELQ----ENVRATLQEEQFKHTLRQCDCEVYQDPAGEADCVIAQALHQREKAYAVFSNDSDFCIFRDCTFIPRTQFDLMGDLQLGAVCDMPIKPERLMVGVITTDRVMAILGLPRHDLLVELSIVGGNDFTGQYIHKGGLQQKLEIKGR-------GIEGVADWIRDHTVPETHPV-FAQEMRHNMGFRGAVVHSRNFYNL----GVPPLKPSKTGHLSQILEKGIREGHFVARVMGMHNNFYWHRAVVEDLSEG-QPCAEVALAPLRAHVYRIVLQR 399          
BLAST of SMED30019528 vs. TrEMBL
Match: A0A2T7PUB9 (Uncharacterized protein OS=Pomacea canaliculata OX=400727 GN=C0Q70_04002 PE=4 SV=1)

HSP 1 Score: 138.272 bits (347), Expect = 3.187e-31
Identity = 110/413 (26.63%), Postives = 200/413 (48.43%), Query Frame = 3
Query:   93 MGIPGLFTFLKRNG--CYETYDLVALAKSNPDLEILVDFYGIEQHILERTLLKLISKYQ-NEYLLIPGAEYDELLNAFRDFHMMFVNIGVKLIYIADGPKGLDKLNQQQKEKSWIYRFERNMRVYKTYLDMCKNPPSPLQKIFEKIIRPCLTSDMFKNIIIKEGGTIINLIYGEADYQIANMRRERN-SWAVLTNDSDFFIFHNCPTIHIGLFDIDRDFMHGRNV---RNPKRLTVGLTSDKILMDRLRLSTHDKLIDFSILMGNDYTVDFSENLRNFCEIKWKYDFSDIPHSMKVAEWVEKQSTKLIDSSEFGSFSIDKPELKLSVQFSMDFYKQIPTEPIPQFTSKDNEEFWNYLVDRYQKGEISSMCLSVFHGFCYQRNCVQPIIVGVNKDINSLLVPLKTRIYSLLLQK 1310
            MGI GL + ++++   C +T +L+ +A     +E++VDFY  E  ++  +L K +  ++ NE L I GAEY  +            ++G+  +++ DG KG    + + K  +W  R   +++  +  L+MC       +   +  IRP L        +   G  +I +  GEAD  IA    ER  ++AVL+NDSDF IF N   I   LFDI+ D   G+++   + P +L  G+ + + +   L+    + L++ SI+ GND+T     +   F +     ++  I   +  A+WV +Q  ++ ++  F    +    L  +V+ S  FY  +  + I +   KD   F N +VDR + G+  +  +S++  F + R  +    +    +   LL PL+  +Y++LL +
Sbjct:    1 MGIRGLMSVIQKHSSECSQTVNLIEIAHEKGGIELVVDFYSFEHLVITDSLWKSLGSFKMNESLRIMGAEYATIDAYISKLITDLKSLGISFVFVLDGSKGSSYADTRHKIDTWKRRHRNDVQRKRDILEMCSGHKLMTELQEDAAIRPVLLEIQVVQALRDAGCEVIQVAEGEADLIIAKQFAEREKAYAVLSNDSDFCIFKNTCFIPKCLFDINGDLGLGQSLALPQKPTQLICGVVASQRVQQFLKFPAVEMLVELSIVAGNDFTGPHMPH--GFADRLQVANYRSI---LSYADWV-RQHVQVENNQVFKQEMMRNRNLANAVEHSRKFYS-LDADLIGE-AGKDGYIF-NLIVDRVKSGQYPANFVSIYRCFYWYRYILDDKYLCAPAE--ELLAPLRADLYAILLPR 402          
BLAST of SMED30019528 vs. TrEMBL
Match: A0A1S3HAE6 (uncharacterized protein LOC106153553 OS=Lingula unguis OX=7574 GN=LOC106153553 PE=4 SV=1)

HSP 1 Score: 134.806 bits (338), Expect = 5.013e-30
Identity = 112/413 (27.12%), Postives = 183/413 (44.31%), Query Frame = 3
Query:   93 MGIPGLFTFL--KRNGCYETYDLVALAKSNPDLEILVDFYGIEQHILERTLLKLISKYQNEYLLIPGAEYDELLNAFRDFHMMFVNIGVKLIYIADGPKGLDKLNQQQKEKSWIYRFERNMRVYKTYLDMCKNPPSPLQKIFEKI-IRPCLTSDMFKNIIIKEGGTIINLIYGEADYQIANMRRER-NSWAVLTNDSDFFIFHNCPTIHIGLFDIDRDFMHGRNVRNPKR---LTVGLTSDKILMDRLRLSTHDKLIDFSILMGNDYTVDFSENLRNFCEIKWKYDFSDIPHSMKVAEWVEKQSTKLIDSSEFGSFSIDKPELKLSVQFSMDFYKQIPTEPIPQFTSKDNEEFWNYLVDRYQKGEISSMCLSVFHGFCYQRNCVQPIIVGVNKDINSLLVPLKTRIYSLLLQK 1310
            MG+ GL ++    +  C E YDL+  A     LEIL+D+Y  +Q IL      L +   N YL + G EY  L            ++G+ +++  DG KG    + QQK ++W  R  + +R  K   D+CK     +Q + + + +RP L        + K    II    GEAD+ IA     R  ++AVL+NDSDF I  N   I   LFD+D     G+    P++   L VG+ + + ++      +H  L+D SI+ GND+T  + ++L N  +++      +    M+  + +E      I + E        P+   +V  S  FY        P      +E    YL    Q G + S  +++   F + R  ++    G+       L  L+T IY ++L +
Sbjct:    1 MGVRGLLSYCLCNQEQCVEYYDLIEEANRKGGLEILIDYYSFQQMILTNFWKSLRAVKHNPYLRLEGGEYSTLDAYLSKLVQDLQSLGIHMVFYIDGAKGSSYFSTQQKLETWKSRHYKELRTLKQLTDVCKGVMD-IQALPDDVNVRPVLLEVQVLGTLKKCNCEIIQNSCGEADFVIAEGLIHRPKAFAVLSNDSDFCIMQNSRFIPNELFDLDNAIQLGQASLLPQKPISLKVGVITTERVLQLFGFRSHRLLVDLSIVNGNDFTAHYMKSLYNLLDVRGAKKVENFAGWMRHYKGIENHP---ILARELQY----NPQFATAVMTSRQFYTLTAPPDRPTMKCPLSE----YLEQAVQHGALPSNIMAMHSNFYWHRFLLEDSTPGL-PCAEVALQSLRTFIYRMVLPR 400          
BLAST of SMED30019528 vs. TrEMBL
Match: K1QJE9 (Uncharacterized protein OS=Crassostrea gigas OX=29159 GN=CGI_10014990 PE=4 SV=1)

HSP 1 Score: 128.257 bits (321), Expect = 7.188e-28
Identity = 87/302 (28.81%), Postives = 141/302 (46.69%), Query Frame = 3
Query:   93 MGIPGLFT--FLKRNGCYETYDLVALAKSNPDLEILVDFYGIEQHILERTLLKLISKYQNEYLLIPGAEYDELLNAFRDFHMMFVNIGVKLIYIADGPKGLDKLNQQQKEKSWIYRFERNMRVYKTYLDMCKNPPSPLQKIFEKIIRPCLTSDMFKNIIIKEGGTIINLIYGEADYQIANMRRER-NSWAVLTNDSDFFIFHNCPTIHIGLFDIDRDFMHGRNVR---NPKRLTVGLTSDKILMDRLRLSTHDKLIDFSILMGNDYTVDF-SENLRNFCEIKWKYDFSDIPHSMKVAEWVEK 977
            MGI GL +    +R  C +  DLVALAKS   +EI+VDFY  E  ++ +    L S   N++L I G EY  L    + F  +F  + +  ++I D  KG  + N QQK  +W+ R  + +      +++C+          +   RP          ++  G  II  + GEAD+ IA    +R  ++A+ +NDSDF +F  C  I   LFDI      G  +     P+ +  G+ S + +   L   +   +++ SI+ GND+T  + S  L    +I+ +            AEWV++
Sbjct:    1 MGIRGLLSHCLRRREECVDEVDLVALAKSRGGIEIIVDFYSFEHMVVPKFWKGLSSLRNNQFLKILGGEYKSLETFIKRFIEIFRQLKISFVFIKDATKGCSEANSQQKLDTWMKRHHKEVENLNEVINVCRGRKEMSNLDEQMFTRPVCLEIQIVETLMSCGCEIIQSVTGEADFMIAKALHDREKAFAIWSNDSDFCVFDKCRFIPNDLFDIYNGLQMGLPIEVPVKPESVWCGIISSEKVRFSLGFQSPHLMVELSIIAGNDFTSQYVSTGLNRQIDIRGRIGVETF------AEWVKR 296          
BLAST of SMED30019528 vs. Ensembl Cavefish
Match: fam120b (family with sequence similarity 120B [Source:ZFIN;Acc:ZDB-GENE-040801-119])

HSP 1 Score: 56.225 bits (134), Expect = 4.928e-8
Identity = 50/203 (24.63%), Postives = 87/203 (42.86%), Query Frame = 3
Query:  279 IPGAEYDELLNAFRDFHMMFVNIGVKLIYIADGPKGLDKLNQQQKEKSWIYRFERN----MRVYKTYLDMCKNPPSPLQKIFEKIIRPCLTSDM--FKNIIIKEGGTIINLIYGEADYQIANMRRERNSWAVLTNDSDFFIFHNCPTIHIGLFDIDRDFMHGRNVRNPKRLTVGLTSDKILMDRLRLSTHDKLIDFSILMGND 869
            + G ++ E ++   +F   F   G++L++  DG        ++QK   W+ R  R      R+++   + C+ P    + +F      CL S +  F    +K  G  +     E DY+IA      N  A+L ND+DF I+   P + I    ++               TV  + +K L + L+L   D L  F+ ++GND
Sbjct:   59 VHGGQWQEYMHFLEEFVGAFTRAGLRLVFFFDGTV------EEQKRAEWVKRRLRVNKDIARMFQYIKNHCQQPD---RDMF------CLPSGLATFSRFALKSLGQEVWCSVREGDYEIAQFALSNNCMAILGNDTDFVIYDTVPYLSIAKLKLE------------SMTTVQFSREK-LCNILKLQKSD-LPLFACILGND 232          
BLAST of SMED30019528 vs. Ensembl Cavefish
Match: fam120b (family with sequence similarity 120B [Source:ZFIN;Acc:ZDB-GENE-040801-119])

HSP 1 Score: 56.225 bits (134), Expect = 5.336e-8
Identity = 50/203 (24.63%), Postives = 87/203 (42.86%), Query Frame = 3
Query:  279 IPGAEYDELLNAFRDFHMMFVNIGVKLIYIADGPKGLDKLNQQQKEKSWIYRFERN----MRVYKTYLDMCKNPPSPLQKIFEKIIRPCLTSDM--FKNIIIKEGGTIINLIYGEADYQIANMRRERNSWAVLTNDSDFFIFHNCPTIHIGLFDIDRDFMHGRNVRNPKRLTVGLTSDKILMDRLRLSTHDKLIDFSILMGND 869
            + G ++ E ++   +F   F   G++L++  DG        ++QK   W+ R  R      R+++   + C+ P    + +F      CL S +  F    +K  G  +     E DY+IA      N  A+L ND+DF I+   P + I    ++               TV  + +K L + L+L   D L  F+ ++GND
Sbjct:   59 VHGGQWQEYMHFLEEFVGAFTRAGLRLVFFFDGTV------EEQKRAEWVKRRLRVNKDIARMFQYIKNHCQQPD---RDMF------CLPSGLATFSRFALKSLGQEVWCSVREGDYEIAQFALSNNCMAILGNDTDFVIYDTVPYLSIAKLKLE------------SMTTVQFSREK-LCNILKLQKSD-LPLFACILGND 232          
BLAST of SMED30019528 vs. Ensembl Sea Lamprey
Match: fam120b (family with sequence similarity 120B [Source:ZFIN;Acc:ZDB-GENE-040801-119])

HSP 1 Score: 57.7658 bits (138), Expect = 4.132e-9
Identity = 47/198 (23.74%), Postives = 83/198 (41.92%), Query Frame = 3
Query:  279 IPGAEYDELLNAFRDFHMMFVNIGVKLIYIADGPKGLDKLNQQQKEKSWIYRFERNMR-VYKTYLDMCKNPPSPLQKIFEKIIRPCLTSDMFKNIIIKEGGTIINLIYGEADYQIANMRRERNSWAVLTNDSDFFIFHNCPTIHIGLFDIDRDFMHGRNVRNPKRLTVGLTSDKILMDRLRLSTHDKLIDFSILMGND 869
            + G ++ E L +  +F     ++G+ L++  DG        QQ K + W  R  +N R +   +  + +    PL  +   +I   L S  F    ++  G  +     EADY++A   R +N   +L  D+D+ ++   P + I   D++              LT  +     L + LR+   D L   + LMGND
Sbjct:   59 VQGGQWKEYLQSLDNFLRRLADVGLHLVFFFDGS------TQQPKRQEWARRRHQNSREISCIFRHIQQTGQQPLDNM--SVIPSGLCS--FTRAALQALGAEVIRTTREADYEVAEFARSKNCIGILGQDTDYLVYDTVPYLSINSLDLE-------------TLTTVMYVRGRLCEHLRIRISD-LPVLACLMGND 232          
BLAST of SMED30019528 vs. Ensembl Medaka
Match: fam120b (family with sequence similarity 120B [Source:NCBI gene;Acc:101166119])

HSP 1 Score: 60.077 bits (144), Expect = 4.113e-9
Identity = 56/223 (25.11%), Postives = 100/223 (44.84%), Query Frame = 3
Query:   93 MGIPGLFTFLKRNGCYETYDLVAL--------AKSNPDLEILVDFYGIEQHILERTLLKLISKYQNEYLLIPGAEYDELLNAFRDFHMMFVNIGVKLIYIADGPKGLDKLNQQQKEKSWIYRFER-NMRVYKTYLDMCKNPPSPLQKIFEKIIRPCLTSDM--FKNIIIKEGGTIINLIYGEADYQIANMRRERNSWAVLTNDSDFFIFHNCPTIHIGLFDID 728
            MG+ GL +F++R  C E    V L        A++  D  ++VD            +  L   Y  E  +  G ++ E ++  + +   F + G++L++  DG      + +Q+K + WI R  R N  V K +  + ++   P +++F      CL S +  F    ++  G  +     EADY+IA+  R+  S  +L  DSDF I+ + P   +    I+
Sbjct:    1 MGVKGLQSFMERC-CPEACVTVNLREMARRHAAQTQQDPTLVVD-----------GMACLCHWYSCEDWVC-GGQWKEYVDVLKSWVDAFTSAGIQLVFFFDG------VVEQRKRQEWIKRRRRVNGDVSKIFRHIKEHGEKPHKELF------CLPSALATFTPFALRFLGQPVFCSVREADYEIASYARQYGSMGILGQDSDFIIYDSVPYFSVAKLRIN 198          
BLAST of SMED30019528 vs. Ensembl Medaka
Match: fam120b (family with sequence similarity 120B [Source:NCBI gene;Acc:101166119])

HSP 1 Score: 60.077 bits (144), Expect = 4.113e-9
Identity = 56/223 (25.11%), Postives = 100/223 (44.84%), Query Frame = 3
Query:   93 MGIPGLFTFLKRNGCYETYDLVAL--------AKSNPDLEILVDFYGIEQHILERTLLKLISKYQNEYLLIPGAEYDELLNAFRDFHMMFVNIGVKLIYIADGPKGLDKLNQQQKEKSWIYRFER-NMRVYKTYLDMCKNPPSPLQKIFEKIIRPCLTSDM--FKNIIIKEGGTIINLIYGEADYQIANMRRERNSWAVLTNDSDFFIFHNCPTIHIGLFDID 728
            MG+ GL +F++R  C E    V L        A++  D  ++VD            +  L   Y  E  +  G ++ E ++  + +   F + G++L++  DG      + +Q+K + WI R  R N  V K +  + ++   P +++F      CL S +  F    ++  G  +     EADY+IA+  R+  S  +L  DSDF I+ + P   +    I+
Sbjct:    1 MGVKGLQSFMERC-CPEACVTVNLREMARRHAAQTQQDPTLVVD-----------GMACLCHWYSCEDWVC-GGQWKEYVDVLKSWVDAFTSAGIQLVFFFDG------VVEQRKRQEWIKRRRRVNGDVSKIFRHIKEHGEKPHKELF------CLPSALATFTPFALRFLGQPVFCSVREADYEIASYARQYGSMGILGQDSDFIIYDSVPYFSVAKLRIN 198          
BLAST of SMED30019528 vs. Planmine SMEST
Match: SMESG000009339.1 (SMESG000009339.1)

HSP 1 Score: 829.706 bits (2142), Expect = 0.000e+0
Identity = 405/417 (97.12%), Postives = 405/417 (97.12%), Query Frame = 3
Query:   93 MGIPGLFTFLKRNGCYETYDLVALAKSNPDLEILVDFYGIEQHILERTLLKLISKYQNEYLLIPGAEYDELLNAFRDFHMMFVNIGVKLIYIADGPKGLDKLNQQQKEKSWIYRFERNMRVYKTYLDMCKNPPSPLQKIFEKIIRPCLTSDMFKNIIIKEGGTIINLIYGEADYQIANMRRERNSWAVLTNDSDFFIFHNCPTIHIGLFDIDRDFMHGRNVR----------NPKRLTVGLTSDKILMDRLRLSTHDKLIDFSILMGNDYTVDFSENLRNFCEIKWKYDFSDIPHSMKVAEWVEKQSTKLIDSSEFGSFSIDKPELKLSVQFSMDFYKQIPTEPIPQFTSKDNEEFWNYLVDRYQKGEISSMCLSVFHGFCYQRNCVQPIIVGVNKDINSLLVPLKTRIYSLLLQKG 1313
            MGIPGLFTFLKRNGCYETYDLVALAKSNPDLEILVDFYGIEQHILERTLLKLISKYQNEYLLIPGAEYDELLNAFRDFHMMFVNIGVKLIYIADGPKGLDKLNQQQKEKSWIYRFERNMRVYKTYLDMCKNPPSPLQKIFEKIIRPCLTSDMFKNIIIKEGGTIINLIYGEADYQIANMRRERNSWAVLTNDSDFFIFHNCPTIHIGLFDIDRDFMHGRNVR          NPKRLTVGLTSDKILMDRLRLSTHDKLIDFSILMGNDYTVDFSENLRNFCEIKWKYDFSDIPHSMKVAEWVEKQSTKLIDSSEF  FSIDKPELKLSVQFSMDFYKQIPTEPIPQFTSKDNEEFWNYLVDRYQKGEISSMCLSVFHGFCYQRNCVQPIIVGVNKDINSLLVPLKTRIYSLLLQKG
Sbjct:    1 MGIPGLFTFLKRNGCYETYDLVALAKSNPDLEILVDFYGIEQHILERTLLKLISKYQNEYLLIPGAEYDELLNAFRDFHMMFVNIGVKLIYIADGPKGLDKLNQQQKEKSWIYRFERNMRVYKTYLDMCKNPPSPLQKIFEKIIRPCLTSDMFKNIIIKEGGTIINLIYGEADYQIANMRRERNSWAVLTNDSDFFIFHNCPTIHIGLFDIDRDFMHGRNVRDNLDDHRPTINPKRLTVGLTSDKILMDRLRLSTHDKLIDFSILMGNDYTVDFSENLRNFCEIKWKYDFSDIPHSMKVAEWVEKQSTKLIDSSEFERFSIDKPELKLSVQFSMDFYKQIPTEPIPQFTSKDNEEFWNYLVDRYQKGEISSMCLSVFHGFCYQRNCVQPIIVGVNKDINSLLVPLKTRIYSLLLQKG 417          
BLAST of SMED30019528 vs. Planmine SMEST
Match: SMESG000001486.1 (SMESG000001486.1)

HSP 1 Score: 164.851 bits (416), Expect = 4.887e-44
Identity = 109/345 (31.59%), Postives = 184/345 (53.33%), Query Frame = 3
Query:   93 MGIPGLFTFLKRNG-CYETYDLVALAKSNPDLEILVDFYGIEQHILERTLLKLISKYQNEYLLIPGAEYDELLNAFRDFHMMFVNIGVKLIYIADGPKGLDKLNQQQKEKSWIYRFERNMRVYKTYLD--MCKNPPSPLQKIFEKIIRPCLTSDMFKNIIIKEGGTIINLIYGEADYQIANMRRERNSWAVLTNDSDFFIFHNC-------PTIHIG----LFDIDRDFMHGRNVRNPKRLTVGLTSDKILMDRLRLSTHDKLIDFSILMGNDYTVDFSENLRNFCE-IKWKYDFSDIPHSMKVAEWVEKQST---KLIDSSEFGSFSIDKPELKLSVQFSMDFY 1073
            MGI GLFT +  +   Y  +DLV  AK    +EIL+DF+  ++H++E   +K++ +Y NEYLL    EYD    AF +FH +F +IG+K ++I DGPKG+D+L   +K ++WI R ++++   K  LD  + K+PP  L       + P L  ++    + K G  II L+ G +D  IA+   +R S+AV+T D+DF +F +C        TIH G    L  ID +   G+  + P      + + K +      +++D+++  SI+ GND T D+++ L+ + + IK +   + + H   VA ++ K       +        F  + P+ + S++F  +FY
Sbjct:    1 MGIRGLFTMIANDERAYTMFDLVKEAKDRDGIEILIDFHAFQRHLVEIISIKMVQEYDNEYLLFSSGEYDIFSKAFSEFHKIFSDIGIKFVWIIDGPKGVDELASTRKTETWIKRDKQDIAQLKGVLDGTLKKSPPKDLNN-----LGPMLGFELSIESLRKCGAEIIQLLQGGSDEFIASEFLKRKSFAVITGDTDFLVFRDCIVIFPRLMTIHYGDTLKLPFIDNE-NPGQIFKTPASFHCPVITHKEMYKLFNFTSYDQIVALSIIKGNDNTEDYAQKLQFYLKIIKNQTKHAHVTH---VANYIVKNKVTPDNIFRLPGMDRFLNENPQCRESIKFCYEFY 336          
BLAST of SMED30019528 vs. Planmine SMEST
Match: SMESG000001486.1 (SMESG000001486.1)

HSP 1 Score: 155.992 bits (393), Expect = 6.302e-41
Identity = 102/330 (30.91%), Postives = 175/330 (53.03%), Query Frame = 3
Query:  135 CYETYDLVALAKSNPDLEILVDFYGIEQHILERTLLKLISKYQNEYLLIPGAEYDELLNAFRDFHMMFVNIGVKLIYIADGPKGLDKLNQQQKEKSWIYRFERNMRVYKTYLD--MCKNPPSPLQKIFEKIIRPCLTSDMFKNIIIKEGGTIINLIYGEADYQIANMRRERNSWAVLTNDSDFFIFHNC-------PTIHIG----LFDIDRDFMHGRNVRNPKRLTVGLTSDKILMDRLRLSTHDKLIDFSILMGNDYTVDFSENLRNFCE-IKWKYDFSDIPHSMKVAEWVEKQST---KLIDSSEFGSFSIDKPELKLSVQFSMDFY 1073
             Y  +DLV  AK    +EIL+DF+  ++H++E   +K++ +Y NEYLL    EYD    AF +FH +F +IG+K ++I DGPKG+D+L   +K ++WI R ++++   K  LD  + K+PP  L       + P L  ++    + K G  II L+ G +D  IA+   +R S+AV+T D+DF +F +C        TIH G    L  ID +   G+  + P      + + K +      +++D+++  SI+ GND T D+++ L+ + + IK +   + + H   VA ++ K       +        F  + P+ + S++F  +FY
Sbjct:    8 AYTMFDLVKEAKDRDGIEILIDFHAFQRHLVEIISIKMVQEYDNEYLLFSSGEYDIFSKAFSEFHKIFSDIGIKFVWIIDGPKGVDELASTRKTETWIKRDKQDIAQLKGVLDGTLKKSPPKDLNN-----LGPMLGFELSIESLRKCGAEIIQLLQGGSDEFIASEFLKRKSFAVITGDTDFLVFRDCIVIFPRLMTIHYGDTLKLPFIDNE-NPGQIFKTPASFHCPVITHKEMYKLFNFTSYDQIVALSIIKGNDNTEDYAQKLQFYLKIIKNQTKHAHVTH---VANYIVKNKVTPDNIFRLPGMDRFLNENPQCRESIKFCYEFY 328          
BLAST of SMED30019528 vs. Planmine SMEST
Match: SMESG000033476.1 (SMESG000033476.1)

HSP 1 Score: 147.517 bits (371), Expect = 3.206e-38
Identity = 117/427 (27.40%), Postives = 197/427 (46.14%), Query Frame = 3
Query:   93 MGIPGLFTFLKRN-GCYETYDLVALAKSNPDLEILVDFYGIEQHILERTLLKLISKYQNEYLLIPGAEYDELLNAFRDFHMMFVNIGVKLIYIADGPKGLDKLNQQQKEKSWIYRFERNMRVYKTYLDMCKNPPSPLQKIFEKIIRPCLTSDMFKNIIIKEGGTIINLIYGEADYQIANMRRERNSWAVLTNDSDFFIFHNCPTIHIGLFDIDRDFMH-----------GRNVRNPKRLTVGLTSDKILMDRLRLSTHDKLIDFSILMGNDYTVDFSENLRNFCEIKWKYDFSDIPHSMKVAEWVEKQST---KLIDSSEFGSFSIDKPELKLSVQFSMDFYKQIPTEPIPQFT------SKDNEEFWNYLVDRYQKGEI-SSMCLSVFHGFCYQRNCVQPIIVGVNKDINSLLVPLKTRIYSLLLQ 1307
            MGI GL+++   +   Y  ++LV  AK   D+EILVDF    + + +  L K+I  + N YL I   E D    A+ +FH MF  IG+KL++I DG   LD++   QK ++W  R +    +   +L+ C+N   P + +    +R           +   G  +I L+ G +D  IA+    R S+A+ +ND+DF +F +C  I   +    RD  H           G+  + P +    + +   +   L+ STH+++I  SIL GND T ++S+NL     I    D    PH  +VA++++        L D     +F  + PE K +V F  DFY     E     T         +    ++L  +    EI   +  S F  + Y+    +PI+  +   + ++L  ++++IY  +L 
Sbjct:    1 MGIRGLYSYFAFDMKAYTIFNLVQKAKVVKDMEILVDFRSFRRDLDKLILRKIIKDFDNNYLSISCVECDIFGKAYLEFHKMFAEIGIKLVWIYDGINSLDEVMCYQKSETWDCRIKNYSNLVDKFLECCQN-KMPFKDLGN--VRSMFNVLTALENLKASGAEVIKLLQGGSDEFIASEFHRRQSYAIFSNDTDFLVFKDCRVIFPRIL---RDIYHDKLPFMDTENPGKFFKKPTKFDCPIVTQSDVFRPLQFSTHEEIIILSILKGNDNTHNYSQNLLYALGIAESLD--RWPHISRVAKYIKSNHVNRNNLFDLDGMSTFLNENPEFKNTVNFCFDFYTLRHFERASGKTLINFDPGSADGSLTSFLQRKLSNMEILDPLYGSHFGMYTYKLVIEKPIVASLT--VGAVLSEIRSKIYPYVLN 417          
BLAST of SMED30019528 vs. Planmine SMEST
Match: SMESG000033479.1 (SMESG000033479.1)

HSP 1 Score: 146.362 bits (368), Expect = 6.433e-38
Identity = 115/393 (29.26%), Postives = 193/393 (49.11%), Query Frame = 3
Query:  183 LEILVDFYGIEQHILERTLLKLISKYQNEYLLIPGAEYDELLNAFRDFHMMFVNIGVKLIYIADGPKGLDKLNQQQKEKSWIYRFERNMRVYKTYLDMCKNPPSPLQKIFEKIIRPCLTSDMFKNIIIKEGGTIINLIYGEADYQIANMRRERNSWAVLTNDSDFFIFHNC----PTIHIGLFDIDRDFMHGRNVRN----PKRLTVGLTSDKILMDRLRLSTHDKLIDFSILMGNDYTVDFSENLRNFCEIKWKYDFSDIPHSMKVAEWVEKQST---KLIDSSEFGSFSIDKPELKLSVQFSMDFY-----KQIPTEPIPQF-TSKDNEEFWNYLVDRYQKGEIS-SMCLSVFHGFCYQRNCVQPIIVGVNKDINSLLVPLKTRIYSLLLQ 1307
            +EILVDF    + + E  L K+I+ + N++L I   E D    A+ +FH MF  IG+KL++I DG KGLD++   +K ++W YR  +        L+ CKN   P++  F+ +I    +    +N +   G  +I L+ G +D  IA+    R S+A+ +ND+DF +F +C    P I   ++  +  FM   N  N    P +L   + +   +   L+ STH+++I  SIL GND T ++S+NL     I    D    PH  +VA++++        L D     +F  + PE K +V F  DFY     ++   + +  F     +    ++L  +  K EIS S+ +S F  +       +PI       I +++  +++RIY  +L 
Sbjct:    1 MEILVDFSSFRRGLDEIILRKIINDFDNDFLSISCVECDIFSKAYLEFHRMFAEIGIKLVWIYDGIKGLDEVVHNEKSETWSYRINKYSNSVDAVLECCKN-KMPVKN-FKDVISWAYSLTALEN-LKASGVEVIKLLQGGSDEFIASEFHRRQSYAIFSNDTDFLVFKDCRVIFPRILRDIYQDELPFMDTENPGNFFKRPTKLNCPIVTQSDVFRPLQFSTHEEIIILSILKGNDNTHNYSKNLLYALGIAESLD--RWPHISRVAKYIKSNHVNRNNLFDLDGMSTFLNENPEFKNTVNFCFDFYTLRHFERASGKTLINFDPGSADGSLTSFLQRKLSKMEISDSLYISHFGRYSCVFWIEKPI--DAFATITTVMSEIRSRIYPYVLH 386          
The following BLAST results are available for this feature:
BLAST of SMED30019528 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
FAM120B6.516e-827.27family with sequence similarity 120B [Source:HGNC ... [more]
FAM120B6.516e-827.27family with sequence similarity 120B [Source:HGNC ... [more]
FAM120B6.546e-827.27family with sequence similarity 120B [Source:HGNC ... [more]
FAM120B6.546e-827.27family with sequence similarity 120B [Source:HGNC ... [more]
FAM120B6.827e-827.27family with sequence similarity 120B [Source:HGNC ... [more]
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BLAST of SMED30019528 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019528 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019528 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
fam120b6.759e-823.31family with sequence similarity 120B [Source:ZFIN;... [more]
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BLAST of SMED30019528 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
fam120b1.154e-625.94Xenopus tropicalis family with sequence similarity... [more]
fam120b1.166e-625.94Xenopus tropicalis family with sequence similarity... [more]
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BLAST of SMED30019528 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Fam120b1.166e-726.42family with sequence similarity 120, member B [Sou... [more]
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BLAST of SMED30019528 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 3
Match NameE-valueIdentityDescription
sp|Q96EK7|F120B_HUMAN3.144e-727.27Constitutive coactivator of peroxisome proliferato... [more]
sp|A6QNT4|F120B_BOVIN4.180e-725.83Constitutive coactivator of peroxisome proliferato... [more]
sp|Q6RI63|F120B_MOUSE8.159e-726.42Constitutive coactivator of peroxisome proliferato... [more]
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BLAST of SMED30019528 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0L8GFX05.334e-3327.25Uncharacterized protein OS=Octopus bimaculoides OX... [more]
A0A210QAR52.809e-3126.20Uncharacterized protein OS=Mizuhopecten yessoensis... [more]
A0A2T7PUB93.187e-3126.63Uncharacterized protein OS=Pomacea canaliculata OX... [more]
A0A1S3HAE65.013e-3027.12uncharacterized protein LOC106153553 OS=Lingula un... [more]
K1QJE97.188e-2828.81Uncharacterized protein OS=Crassostrea gigas OX=29... [more]
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BLAST of SMED30019528 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
fam120b4.928e-824.63family with sequence similarity 120B [Source:ZFIN;... [more]
fam120b5.336e-824.63family with sequence similarity 120B [Source:ZFIN;... [more]
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BLAST of SMED30019528 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
fam120b4.132e-923.74family with sequence similarity 120B [Source:ZFIN;... [more]
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BLAST of SMED30019528 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019528 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019528 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
fam120b4.113e-925.11family with sequence similarity 120B [Source:NCBI ... [more]
fam120b4.113e-925.11family with sequence similarity 120B [Source:NCBI ... [more]
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BLAST of SMED30019528 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000009339.10.000e+097.12SMESG000009339.1[more]
SMESG000001486.14.887e-4431.59SMESG000001486.1[more]
SMESG000001486.16.302e-4130.91SMESG000001486.1[more]
SMESG000033476.13.206e-3827.40SMESG000033476.1[more]
SMESG000033479.16.433e-3829.26SMESG000033479.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30019528 ID=SMED30019528|Name=SMED30019528|organism=Schmidtea mediterranea sexual|type=transcript|length=1506bp
TACTTTTAAAGATCCGTTTTAAAATTGCATTTATTAGAATTTTATTTTAT
TTTATATTTGTAAAATAAAAATATTAAGTTTTATTATTTATAATGGGGAT
TCCTGGGTTGTTCACGTTTTTAAAAAGAAATGGATGTTATGAAACTTATG
ACTTGGTTGCATTAGCGAAAAGTAATCCGGATCTTGAAATCCTTGTTGAT
TTTTATGGTATTGAGCAGCATATATTGGAAAGAACATTGCTGAAACTTAT
TAGCAAATATCAGAATGAATATTTATTGATTCCCGGTGCAGAGTACGATG
AACTTCTAAATGCTTTTCGAGATTTTCATATGATGTTTGTTAATATTGGT
GTTAAATTAATTTATATTGCTGATGGACCAAAAGGACTTGATAAATTGAA
CCAACAACAAAAAGAAAAATCATGGATTTATCGTTTTGAAAGAAATATGC
GAGTATATAAAACTTATTTGGATATGTGTAAAAATCCGCCTAGTCCATTG
CAGAAAATTTTTGAAAAAATTATCCGACCATGTTTGACATCTGACATGTT
TAAAAACATAATTATCAAAGAAGGGGGAACAATTATCAATTTAATTTATG
GTGAAGCCGATTATCAAATTGCCAATATGCGCAGAGAACGAAATTCATGG
GCCGTGTTGACAAACGATAGCGATTTTTTTATTTTTCACAATTGTCCAAC
AATACACATTGGGCTTTTTGACATTGACAGAGATTTTATGCACGGCCGTA
ATGTCCGTAATCCGAAAAGGTTGACAGTTGGATTGACATCCGATAAAATT
TTAATGGATCGTTTGCGATTGTCAACGCATGATAAATTAATTGATTTTTC
AATTTTAATGGGCAATGATTATACTGTGGATTTCTCGGAGAATTTGAGGA
ATTTTTGCGAAATTAAATGGAAATATGATTTCAGTGATATTCCACATTCG
ATGAAAGTGGCAGAATGGGTTGAAAAGCAATCAACTAAACTGATAGACAG
CAGTGAATTTGGAAGCTTTTCGATAGATAAGCCAGAACTGAAATTGTCAG
TTCAATTCAGTATGGATTTCTACAAGCAAATTCCAACTGAACCAATTCCA
CAATTCACGTCGAAAGACAACGAAGAATTTTGGAATTATCTAGTCGATCG
TTATCAGAAGGGAGAAATATCTAGCATGTGTTTATCGGTTTTCCATGGAT
TCTGTTATCAAAGAAATTGTGTTCAGCCAATAATTGTTGGCGTTAACAAA
GACATTAATAGTTTGTTAGTGCCGTTGAAAACGAGAATTTATTCGCTTTT
GCTTCAAAAAGGGTGAATTTTATTTAATATTTTATGTCGTGTATTAATGT
AGAATTACGATATAAAATCTTACATGTGTGGATTGGGTGGAAATACTAAA
TTCACTTTGACTTCTATATGATTTGTAGAATATTCTTGATTGTTATATTT
ACAAATTGTTTAATATATTTTTATTGATACCTTTATTAATCAATTAACTG
ATAATT
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protein sequence of SMED30019528-orf-1

>SMED30019528-orf-1 ID=SMED30019528-orf-1|Name=SMED30019528-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=408bp
MGIPGLFTFLKRNGCYETYDLVALAKSNPDLEILVDFYGIEQHILERTLL
KLISKYQNEYLLIPGAEYDELLNAFRDFHMMFVNIGVKLIYIADGPKGLD
KLNQQQKEKSWIYRFERNMRVYKTYLDMCKNPPSPLQKIFEKIIRPCLTS
DMFKNIIIKEGGTIINLIYGEADYQIANMRRERNSWAVLTNDSDFFIFHN
CPTIHIGLFDIDRDFMHGRNVRNPKRLTVGLTSDKILMDRLRLSTHDKLI
DFSILMGNDYTVDFSENLRNFCEIKWKYDFSDIPHSMKVAEWVEKQSTKL
IDSSEFGSFSIDKPELKLSVQFSMDFYKQIPTEPIPQFTSKDNEEFWNYL
VDRYQKGEISSMCLSVFHGFCYQRNCVQPIIVGVNKDINSLLVPLKTRIY
SLLLQKG*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000014zeta neoblast
PLANA:0000429neoblast
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR026832Asteroid
IPR006086XPG-I_dom
IPR029060PIN-like_dom_sf
Vocabulary: molecular function
TermDefinition
GO:0004518nuclease activity
Vocabulary: cellular component
TermDefinition
GO:0005634nucleus
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006086XPG-I domainPFAMPF00867XPG_Icoord: 170..269
e-value: 8.9E-7
score: 29.2
NoneNo IPR availableGENE3DG3DSA:3.40.50.1010coord: 39..279
e-value: 7.4E-13
score: 50.3
NoneNo IPR availableCDDcd18676PIN_asteroid-likecoord: 65..198
e-value: 5.28956E-6
score: 44.1724
IPR026832AsteroidPANTHERPTHR15665ASTEROID PROTEINcoord: 1..379
IPR029060PIN-like domain superfamilySUPERFAMILYSSF88723PIN domain-likecoord: 1..220