SMED30019508

Overview
NameSMED30019508
Smed IDSMED30019508
Length (bp)2981
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30019508 (SMED30019508) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30019508 (SMED30019508) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30019508 (SMED30019508) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30019508 (SMED30019508) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30019508

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30019508SMESG000067154.1 SmedASXL_019847SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30019508SMESG000067154.1 SmedASXL_019847SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X1 cellSMED30019508SMESG000067154.1 SmedASXL_000626SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30019508SMESG000067154.1 SmedASXL_000626SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
neoblastSMED30019508SMESG000067154.1 dd_Smed_v4_6479_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30019508SMESG000067154.1 dd_Smed_v4_6479_1_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30019508SMESG000067154.1 Contig47940uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30019508SMESG000067154.1 Contig47940newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30019508 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000515:102103..105160 -14863
Homology
[Close]
BLAST of SMED30019508 vs. Planmine SMEST
Match: SMESG000067154.1 (SMESG000067154.1)

HSP 1 Score: 1665.59 bits (4312), Expect = 0.000e+0
Identity = 912/915 (99.67%), Postives = 913/915 (99.78%), Query Frame = 1 
Query:   13 MPTGGRINRRDKLRSKTLLTNTKDKDNDVKQTDSIKTVSGFLKNAKTKSSKVNVVSGNKIDINESEIISKGLRKFYVCLEDIKLNNENDKDISDKVNVKKMNKTLLKPESINISTESPKKNDEIVVENLYDTLKRKGRHXXXXXXXXXXVPVVEKVDKLETSIIKIEIPKKRGRKKKEENDAVISNTNQKDELNTTLADKEKVDSKIELDEESSIKQSKLVRRNALTKTQEQKVDIQLIVQXXXXXXXXXXXXXXXXTDVDLDVDNKKSRARRNTTSKTIENKAETIEQVDEEKSTVQRTRSRLPKAKKPFVVEKEQANPEETKIPGLAMLANIKNRKSKMIKDKEELMSKESIETHEKPQSKSKEITKPIENPSMKIPGLRMLADLKQRKSELIGSSNRDATHEFIKPILPKIXXXXXXXXXXXXXXPGTDNMSPVANIKPVVVHQVTPIPPRQSAVRKSKLAPMVSDKVPGLLITEELKKRKSEMMSEQSNNARRSETAEKKKPREIAPKIAADSSVVPVTPIAPRQNAARKSKLAPMASDKVPGLQDTEIIKKRKSESIPEPSPAKIAPKPAVIDSIXXXXXXXXXXGTIHSPTQFSATKSALVSRSETEIDKPKPTKPLQKSATLDSNFLRSPYRPPHVHPDNQDIPSTPSALDSSLANIRFISMKSPVGILSKPSPFKRPISTLDFSGDGNGGNYEDELNSRCKIPKLQTENRRQSVTFNREVSVKEIHGNSFNSVNSDEASVSSDERIDDFEHRLNEEFERVRNKVAEKHNISFSXXXXXXXXXNLHSNEFLKERQRVIGPSAFSRTDSKTSSDLSGWISNRIKIQSNSPPRERPQSRALNFDQSRSGALKKVNPPIANQQKDDFPSNWRSFDQVSAQPPKLGTDSDGDNSMEVDEKEDETSKSKCSIM 2757
            MPTGGRINRRDKLRSKTLLTNTKDKDNDVKQTDSIKTVSGFLKNAKTKSSKVNVVSGNKIDINESEIISKGLRKFYVCLEDIKLNNENDKDISDKVNVKKMNKTLLKPESINISTESPKKNDEIVVENLYDTLKRKGRHTKTNNTNNFTVPVVEKVDKLETSIIKIEIPKKRGRKKKEENDAVISNTNQKDELNTTLADKEKVDSKIELDEESSIKQSKLVRRNALTKTQEQKVDIQLIVQKIEID+ESESKSEKSNTDVDLDVDNKKSRARRNTTSKTIENKAETIEQVDEEKSTVQRTRSRLPKAKKPFVVEKEQANPEETKIPGLAMLANIKNRKSKMIKDKEELMSKESIETHEKPQSKSKEITKPIENPSMKIPGLRMLADLKQRKSELIGSSNRDATHEFIKPILPKISDKKTSQSKESEKKPGTDNMSPVANIKPVVVHQVTPIPPRQSAVRKSKLAPMVSDKVPGLLITEELKKRKSEMMSEQSNNARRSETAEKKKPREIAPKIAADSSVVPVTPIAPRQNAARKSKLAPM SDKVPGLQDTEIIKKRKSESIPEPSPAKIAPKPAVIDSIRAASAASLARGTIHSPTQFSATKSALVSRSETEIDKPKPTKPLQKSATLDSNFLRSPYRPPHVHPDNQDIPSTPSALDSSLANIRFISMKSPVGILSKPSPFKRPISTLDFSGDGNGGNYEDELNSRCKIPKLQTENRRQSVTFNREVSVKEIH NSFNSVNSDEASVSSDERIDDFEHRLNEEFERVRNKVAEKHNISFSTGTGGTAAGNLHSNEFLKERQRVIGPSAFSRTDSKTSSDLSGWISNRIKIQSNSPPRERPQSRALNFDQSRSGALKKVNPPIANQQKDDFPSNWRSFDQVSAQPPKLGTDSDGDNSMEVDEKEDETSKSKCSIM
Sbjct:    1 MPTGGRINRRDKLRSKTLLTNTKDKDNDVKQTDSIKTVSGFLKNAKTKSSKVNVVSGNKIDINESEIISKGLRKFYVCLEDIKLNNENDKDISDKVNVKKMNKTLLKPESINISTESPKKNDEIVVENLYDTLKRKGRHTKTNNTNNFTVPVVEKVDKLETSIIKIEIPKKRGRKKKEENDAVISNTNQKDELNTTLADKEKVDSKIELDEESSIKQSKLVRRNALTKTQEQKVDIQLIVQKIEIDDESESKSEKSNTDVDLDVDNKKSRARRNTTSKTIENKAETIEQVDEEKSTVQRTRSRLPKAKKPFVVEKEQANPEETKIPGLAMLANIKNRKSKMIKDKEELMSKESIETHEKPQSKSKEITKPIENPSMKIPGLRMLADLKQRKSELIGSSNRDATHEFIKPILPKISDKKTSQSKESEKKPGTDNMSPVANIKPVVVHQVTPIPPRQSAVRKSKLAPMVSDKVPGLLITEELKKRKSEMMSEQSNNARRSETAEKKKPREIAPKIAADSSVVPVTPIAPRQNAARKSKLAPMVSDKVPGLQDTEIIKKRKSESIPEPSPAKIAPKPAVIDSIRAASAASLARGTIHSPTQFSATKSALVSRSETEIDKPKPTKPLQKSATLDSNFLRSPYRPPHVHPDNQDIPSTPSALDSSLANIRFISMKSPVGILSKPSPFKRPISTLDFSGDGNGGNYEDELNSRCKIPKLQTENRRQSVTFNREVSVKEIHSNSFNSVNSDEASVSSDERIDDFEHRLNEEFERVRNKVAEKHNISFSTGTGGTAAGNLHSNEFLKERQRVIGPSAFSRTDSKTSSDLSGWISNRIKIQSNSPPRERPQSRALNFDQSRSGALKKVNPPIANQQKDDFPSNWRSFDQVSAQPPKLGTDSDGDNSMEVDEKEDETSKSKCSIM 915
[Close]
BLAST of SMED30019508 vs. Planmine SMEST
Match: SMESG000067154.1 (SMESG000067154.1)

HSP 1 Score: 1643.63 bits (4255), Expect = 0.000e+0
Identity = 895/915 (97.81%), Postives = 896/915 (97.92%), Query Frame = 1 
Query:   13 MPTGGRINRRDKLRSKTLLTNTKDKDNDVKQTDSIKTVSGFLKNAKTKSSKVNVVSGNKIDINESEIISKGLRKFYVCLEDIKLNNENDKDISDKVNVKKMNKTLLKPESINISTESPKKNDEIVVENLYDTLKRKGRHXXXXXXXXXXVPVVEKVDKLETSIIKIEIPKKRGRKKKEENDAVISNTNQKDELNTTLADKEKVDSKIELDEESSIKQSKLVRRNALTKTQEQKVDIQLIVQXXXXXXXXXXXXXXXXTDVDLDVDNKKSRARRNTTSKTIENKAETIEQVDEEKSTVQRTRSRLPKAKKPFVVEKEQANPEETKIPGLAMLANIKNRKSKMIKDKEELMSKESIETHEKPQSKSKEITKPIENPSMKIPGLRMLADLKQRKSELIGSSNRDATHEFIKPILPKIXXXXXXXXXXXXXXPGTDNMSPVANIKPVVVHQVTPIPPRQSAVRKSKLAPMVSDKVPGLLITEELKKRKSEMMSEQSNNARRSETAEKKKPREIAPKIAADSSVVPVTPIAPRQNAARKSKLAPMASDKVPGLQDTEIIKKRKSESIPEPSPAKIAPKPAVIDSIXXXXXXXXXXGTIHSPTQFSATKSALVSRSETEIDKPKPTKPLQKSATLDSNFLRSPYRPPHVHPDNQDIPSTPSALDSSLANIRFISMKSPVGILSKPSPFKRPISTLDFSGDGNGGNYEDELNSRCKIPKLQTENRRQSVTFNREVSVKEIHGNSFNSVNSDEASVSSDERIDDFEHRLNEEFERVRNKVAEKHNISFSXXXXXXXXXNLHSNEFLKERQRVIGPSAFSRTDSKTSSDLSGWISNRIKIQSNSPPRERPQSRALNFDQSRSGALKKVNPPIANQQKDDFPSNWRSFDQVSAQPPKLGTDSDGDNSMEVDEKEDETSKSKCSIM 2757
            MPTGGRINRRDKLRSKTLLTNTKDKDNDVKQTDSIKTVSGFLKNAKTKSSKVNVVSGNKIDINESEIISKGLRKFYVCLEDIKLNNENDKDISDKVNVKKMNKTLLKPESINISTESPKKNDEIVVENLYDTLKRK                 EKVDKLETSIIKIEIPKKRGRKKKEENDAVISNTNQKDELNTTLADKEKVDSKIELDEESSIKQSKLVRRNALTKTQEQKVDIQLIVQKIEID+ESESKSEKSNTDVDLDVDNKKSRARRNTTSKTIENKAETIEQVDEEKSTVQRTRSRLPKAKKPFVVEKEQANPEETKIPGLAMLANIKNRKSKMIKDKEELMSKESIETHEKPQSKSKEITKPIENPSMKIPGLRMLADLKQRKSELIGSSNRDATHEFIKPILPKISDKKTSQSKESEKKPGTDNMSPVANIKPVVVHQVTPIPPRQSAVRKSKLAPMVSDKVPGLLITEELKKRKSEMMSEQSNNARRSETAEKKKPREIAPKIAADSSVVPVTPIAPRQNAARKSKLAPM SDKVPGLQDTEIIKKRKSESIPEPSPAKIAPKPAVIDSIRAASAASLARGTIHSPTQFSATKSALVSRSETEIDKPKPTKPLQKSATLDSNFLRSPYRPPHVHPDNQDIPSTPSALDSSLANIRFISMKSPVGILSKPSPFKRPISTLDFSGDGNGGNYEDELNSRCKIPKLQTENRRQSVTFNREVSVKEIH NSFNSVNSDEASVSSDERIDDFEHRLNEEFERVRNKVAEKHNISFSTGTGGTAAGNLHSNEFLKERQRVIGPSAFSRTDSKTSSDLSGWISNRIKIQSNSPPRERPQSRALNFDQSRSGALKKVNPPIANQQKDDFPSNWRSFDQVSAQPPKLGTDSDGDNSMEVDEKEDETSKSKCSIM
Sbjct:    1 MPTGGRINRRDKLRSKTLLTNTKDKDNDVKQTDSIKTVSGFLKNAKTKSSKVNVVSGNKIDINESEIISKGLRKFYVCLEDIKLNNENDKDISDKVNVKKMNKTLLKPESINISTESPKKNDEIVVENLYDTLKRK-----------------EKVDKLETSIIKIEIPKKRGRKKKEENDAVISNTNQKDELNTTLADKEKVDSKIELDEESSIKQSKLVRRNALTKTQEQKVDIQLIVQKIEIDDESESKSEKSNTDVDLDVDNKKSRARRNTTSKTIENKAETIEQVDEEKSTVQRTRSRLPKAKKPFVVEKEQANPEETKIPGLAMLANIKNRKSKMIKDKEELMSKESIETHEKPQSKSKEITKPIENPSMKIPGLRMLADLKQRKSELIGSSNRDATHEFIKPILPKISDKKTSQSKESEKKPGTDNMSPVANIKPVVVHQVTPIPPRQSAVRKSKLAPMVSDKVPGLLITEELKKRKSEMMSEQSNNARRSETAEKKKPREIAPKIAADSSVVPVTPIAPRQNAARKSKLAPMVSDKVPGLQDTEIIKKRKSESIPEPSPAKIAPKPAVIDSIRAASAASLARGTIHSPTQFSATKSALVSRSETEIDKPKPTKPLQKSATLDSNFLRSPYRPPHVHPDNQDIPSTPSALDSSLANIRFISMKSPVGILSKPSPFKRPISTLDFSGDGNGGNYEDELNSRCKIPKLQTENRRQSVTFNREVSVKEIHSNSFNSVNSDEASVSSDERIDDFEHRLNEEFERVRNKVAEKHNISFSTGTGGTAAGNLHSNEFLKERQRVIGPSAFSRTDSKTSSDLSGWISNRIKIQSNSPPRERPQSRALNFDQSRSGALKKVNPPIANQQKDDFPSNWRSFDQVSAQPPKLGTDSDGDNSMEVDEKEDETSKSKCSIM 898
The following BLAST results are available for this feature:
BLAST of SMED30019508 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019508 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019508 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019508 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019508 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019508 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019508 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019508 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019508 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019508 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019508 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019508 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019508 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30019508 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000067154.10.000e+099.67SMESG000067154.1[more]
SMESG000067154.10.000e+097.81SMESG000067154.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30019508 ID=SMED30019508|Name=SMED30019508|organism=Schmidtea mediterranea sexual|type=transcript|length=2981bp
CAATAAATTTAAATGCCCACTGGTGGTCGTATTAATAGAAGGGATAAGTT
AAGAAGTAAAACTCTTTTAACAAATACAAAAGACAAGGACAATGACGTGA
AACAAACTGATTCTATAAAAACTGTTTCAGGTTTTTTAAAAAATGCTAAA
ACTAAAAGTTCTAAAGTTAATGTTGTATCAGGAAATAAAATTGATATAAA
TGAAAGTGAGATAATTAGTAAAGGTTTACGAAAATTTTATGTTTGCTTAG
AAGATATAAAATTAAATAATGAGAATGATAAAGATATTTCTGACAAAGTA
AATGTGAAAAAAATGAATAAGACTTTATTGAAACCCGAATCAATTAACAT
CTCAACTGAGTCACCAAAGAAGAATGATGAAATTGTCGTAGAAAATTTGT
ACGATACTCTTAAACGTAAGGGTAGGCACACTAAAACCAATAATACGAAT
AATTTTACAGTTCCTGTTGTAGAAAAAGTTGATAAACTCGAAACAAGTAT
AATCAAAATTGAAATTCCTAAAAAGCGTGGTAGGAAAAAGAAAGAAGAAA
ATGACGCAGTTATAAGCAATACAAATCAAAAAGATGAATTAAATACCACT
TTAGCGGATAAAGAAAAGGTTGACAGTAAAATAGAGCTCGATGAGGAAAG
TTCAATTAAACAGTCGAAATTAGTTAGGAGAAACGCTCTAACTAAAACTC
AAGAACAAAAAGTTGACATTCAATTGATTGTACAAAAAATAGAAATTGAC
AATGAAAGTGAATCTAAATCAGAAAAAAGCAATACTGACGTTGATTTAGA
TGTCGATAACAAGAAATCTCGTGCTCGTAGGAACACAACGTCGAAGACAA
TTGAAAACAAAGCAGAAACAATCGAACAAGTTGACGAAGAAAAGTCAACT
GTTCAACGAACTCGAAGTCGATTACCAAAAGCAAAGAAACCATTTGTTGT
TGAGAAAGAACAAGCGAATCCAGAGGAAACTAAAATTCCCGGGCTCGCTA
TGCTTGCTAATATTAAAAATCGAAAATCGAAAATGATAAAAGACAAAGAG
GAATTAATGAGCAAGGAATCGATCGAAACGCATGAGAAACCCCAAAGCAA
ATCGAAAGAAATAACGAAACCCATTGAGAATCCATCTATGAAAATTCCCG
GATTGAGAATGTTAGCCGACCTGAAGCAGCGGAAATCAGAGCTGATTGGC
TCATCTAATCGAGACGCCACGCATGAATTCATCAAGCCGATCCTCCCGAA
AATATCGGATAAAAAAACCAGCCAATCAAAAGAAAGCGAAAAGAAACCCG
GAACCGACAATATGTCCCCAGTAGCGAATATTAAACCGGTGGTTGTCCAC
CAAGTTACCCCGATCCCTCCTCGGCAGAGTGCTGTAAGGAAATCTAAACT
GGCCCCAATGGTATCGGATAAGGTTCCGGGACTTCTAATTACCGAGGAGC
TCAAGAAACGGAAATCCGAAATGATGTCGGAACAGAGCAATAATGCCAGG
CGATCTGAAACAGCCGAGAAGAAAAAACCGAGAGAAATCGCCCCTAAAAT
AGCAGCGGATTCAAGCGTGGTGCCGGTTACCCCGATTGCTCCCAGACAGA
ATGCCGCGAGGAAATCCAAATTAGCCCCAATGGCATCGGATAAGGTTCCC
GGGTTACAGGACACAGAAATCATCAAGAAACGTAAATCAGAATCGATTCC
AGAGCCCAGCCCGGCCAAAATAGCCCCGAAACCAGCAGTAATCGATAGCA
TTAGGGCGGCATCAGCCGCAAGTCTGGCCCGAGGCACAATTCACTCACCA
ACGCAATTCAGCGCCACAAAATCTGCGCTGGTGTCGCGATCGGAAACCGA
AATCGATAAACCGAAACCAACAAAACCCCTTCAGAAATCTGCAACACTCG
ACTCAAATTTCCTCCGATCCCCGTATCGACCGCCCCATGTGCACCCTGAC
AACCAGGATATCCCATCAACACCGAGCGCTCTCGATTCATCGTTAGCTAA
TATCCGATTCATCAGCATGAAATCTCCAGTCGGAATCCTATCGAAACCGT
CGCCATTCAAACGTCCGATTTCCACACTCGATTTCAGTGGCGATGGCAAC
GGTGGTAACTATGAGGATGAGCTGAATTCCCGCTGCAAAATACCGAAACT
CCAAACGGAAAATCGCAGACAAAGTGTCACCTTCAATCGGGAGGTCTCGG
TGAAAGAAATCCATGGCAACTCGTTCAATAGCGTCAATTCCGATGAGGCT
TCCGTATCGAGCGATGAGCGTATCGATGATTTCGAGCACCGATTGAATGA
GGAATTTGAACGGGTGAGAAACAAGGTTGCCGAGAAACACAATATCAGCT
TCTCAACTGGCACTGGCGGCACCGCTGCTGGCAATCTCCATTCAAATGAG
TTCCTGAAGGAGCGTCAGCGCGTGATTGGCCCGTCGGCATTCAGTAGGAC
TGACTCAAAAACATCATCGGATTTATCGGGATGGATTTCCAACCGAATCA
AAATCCAGAGCAATTCGCCACCGAGGGAACGGCCGCAAAGTCGAGCCCTC
AATTTCGACCAATCACGATCCGGAGCACTGAAAAAGGTGAATCCACCGAT
CGCAAATCAGCAGAAGGATGATTTCCCTAGCAACTGGAGGAGTTTCGATC
AGGTTTCCGCGCAACCACCGAAACTCGGTACCGATAGTGACGGTGACAAC
TCGATGGAGGTCGACGAGAAAGAGGACGAAACGAGCAAATCCAAATGCTC
AATTATGTGAAATGTTTCCTTTATTAATATTTGATTGATCATTTGTCTCT
TATTTTAATATCTAATTATTATTTATTAATTGAGTGGTGCTCATGTTGCC
ATGTGAACTGCCGTGTAGTTGGATCATGCATTTTATCAGGTTCTGTAATT
GCACTGTAAAGATTTATTATTTCATGTATTAGTCTCATTGTAATTATTAA
TTGCTATCTATCTATCTATATTGTATCAGTG
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protein sequence of SMED30019508-orf-1

>SMED30019508-orf-1 ID=SMED30019508-orf-1|Name=SMED30019508-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=916bp
MPTGGRINRRDKLRSKTLLTNTKDKDNDVKQTDSIKTVSGFLKNAKTKSS
KVNVVSGNKIDINESEIISKGLRKFYVCLEDIKLNNENDKDISDKVNVKK
MNKTLLKPESINISTESPKKNDEIVVENLYDTLKRKGRHTKTNNTNNFTV
PVVEKVDKLETSIIKIEIPKKRGRKKKEENDAVISNTNQKDELNTTLADK
EKVDSKIELDEESSIKQSKLVRRNALTKTQEQKVDIQLIVQKIEIDNESE
SKSEKSNTDVDLDVDNKKSRARRNTTSKTIENKAETIEQVDEEKSTVQRT
RSRLPKAKKPFVVEKEQANPEETKIPGLAMLANIKNRKSKMIKDKEELMS
KESIETHEKPQSKSKEITKPIENPSMKIPGLRMLADLKQRKSELIGSSNR
DATHEFIKPILPKISDKKTSQSKESEKKPGTDNMSPVANIKPVVVHQVTP
IPPRQSAVRKSKLAPMVSDKVPGLLITEELKKRKSEMMSEQSNNARRSET
AEKKKPREIAPKIAADSSVVPVTPIAPRQNAARKSKLAPMASDKVPGLQD
TEIIKKRKSESIPEPSPAKIAPKPAVIDSIRAASAASLARGTIHSPTQFS
ATKSALVSRSETEIDKPKPTKPLQKSATLDSNFLRSPYRPPHVHPDNQDI
PSTPSALDSSLANIRFISMKSPVGILSKPSPFKRPISTLDFSGDGNGGNY
EDELNSRCKIPKLQTENRRQSVTFNREVSVKEIHGNSFNSVNSDEASVSS
DERIDDFEHRLNEEFERVRNKVAEKHNISFSTGTGGTAAGNLHSNEFLKE
RQRVIGPSAFSRTDSKTSSDLSGWISNRIKIQSNSPPRERPQSRALNFDQ
SRSGALKKVNPPIANQQKDDFPSNWRSFDQVSAQPPKLGTDSDGDNSMEV
DEKEDETSKSKCSIM*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000429neoblast
PLANA:0002109X1 cell
PLANA:0002111X2 cell
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 331..351
NoneNo IPR availableCOILSCoilCoilcoord: 280..300
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 827..854
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 347..366
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 549..563
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 7..25
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..25
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 599..631
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 866..891
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 395..437
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 406..430
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 482..511
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 827..915
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 482..573
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 347..379
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 247..279