Vesicle-fusing ATPase

Overview
NameVesicle-fusing ATPase
Smed IDSMED30019484
Length (bp)2372
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Vesicle-fusing ATPase (SMED30019484) t-SNE clustered cells

Violin plots show distribution of expression levels for Vesicle-fusing ATPase (SMED30019484) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Vesicle-fusing ATPase (SMED30019484) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Vesicle-fusing ATPase (SMED30019484) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30019484

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30019484SMESG000019983.1 dd_Smed_v4_3364_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30019484SMESG000019983.1 dd_Smed_v4_3364_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30019484SMESG000019983.1 dd_Smed_v4_3364_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult colorimetric in situ hybridization evidence
neoblastSMED30019484SMESG000019983.1 dd_Smed_v4_3364_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30019484SMESG000019983.1 dd_Smed_v4_3364_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30019484SMESG000019983.1 Contig48532uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30019484SMESG000019983.1 Contig48532newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Vesicle-fusing ATPase vs. Ensembl Human
Match: NSF (N-ethylmaleimide sensitive factor, vesicle fusing ATPase [Source:HGNC Symbol;Acc:HGNC:8016])

HSP 1 Score: 582.408 bits (1500), Expect = 0.000e+0
Identity = 292/661 (44.18%), Postives = 429/661 (64.90%), Query Frame = 2
Query:   35 DEFSYTNKIFFNPSDWALFKEKFITVTTASNKFIFKSDKDESVKKGCMTTNGIQRKWMYCGIGEVVECIPYSFQXXXXXXXXXXXXDLFVKSASL-KDPIDSDKLAVKYLERFHDTPVTKNQLLLFNWESINFEAKIISIQGLDVK-------SSKMNEIETGIIQSNTVFKFQRRPESKFMLSGRCKAENAAVCLINPNWDFNSIGVGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEKFDPKKGIAIRIEDLQSFKINRQHFIFAIDHDVKALYGRHDDDLQEFKENRLLKWSPQISKALSTAGLLIESLKTNDNISRKRLLIYGEPLTGTSSVAVHLAKETGFTFIRMCSMRNMIGFSEPARCLAIKKIFDDALKAPASVIILDDVERLIDYSSIGPRFSIFLVNNLIGMINCFVPKNKKMLIIGTTKHLSAMHELEFFSAFDKNCIKIPEIKT 1993
            DE S TN    N  D+    +  I  T+ ++++ F      SV  G +  +  QRKW    IG+ +E   Y+F         +     F++  S+  +P D+DK+A +++++F++   +  Q L+F++    F   +  I+ +D         + K  +IE G++  N+   F++   S   L G+ K +     +INP+W+F  +G+GGLD+EFS IF+ AF SR+FPPE VE++G KHV+GILLYGPPG GKTL ARQIGKMLNAREPK+VNGP IL+KYVGESEANIRKLFADAEEE +++G  S LHIIIFDEIDAICK RG+++G + V DTVVNQLL+K+DGVEQLNNIL+IGMTNR D+IDEALLRPGR+ V++E+ LP + GRLQI  IHT ++R   +L+ D+D+KELA  TKN+SGAE+E +V AA + A +        + + +E  +S ++ R  F+ ++++D+K  +G + +D   +  N ++KW   +++ L    LL++  K +D      +L+ G P +G +++A  +A+E+ F FI++CS   MIGFSE A+C A+KKIFDDA K+  S +++DD+ERL+DY  IGPRFS  ++  L+ ++    P+ +K+LIIGTT     + E+E  +AF    I +P I T
Sbjct:    9 DELSLTNCAVVNEKDFQ-SGQHVIVRTSPNHRYTFTLKTHPSVVPGSIAFSLPQRKWAGLSIGQEIEVSLYTFDKAKQCIGTMTIEIDFLQKKSIDSNPYDTDKMAAEFIQQFNNQAFSVGQQLVFSFNEKLFGLLVKDIEAMDPSILKGEPATGKRQKIEVGLVVGNSQVAFEKAENSSLNLIGKAKTKENRQSIINPDWNFEKMGIGGLDKEFSDIFRRAFASRVFPPEIVEQMGCKHVKGILLYGPPGCGKTLLARQIGKMLNAREPKVVNGPEILNKYVGESEANIRKLFADAEEEQRRLGANSGLHIIIFDEIDAICKQRGSMAGSTGVHDTVVNQLLSKIDGVEQLNNILVIGMTNRPDLIDEALLRPGRLEVKMEIGLPDEKGRLQILHIHTARMRGHQLLSADVDIKELAVETKNFSGAELEGLVRAAQSTAMNRHIKASTKVEVDMEKAESLQVTRGDFLASLENDIKPAFGTNQEDYASYIMNGIIKWGDPVTRVLDDGELLVQQTKNSDRTPLVSVLLEGPPHSGKTALAAKIAEESNFPFIKICSPDKMIGFSETAKCQAMKKIFDDAYKSQLSCVVVDDIERLLDYVPIGPRFSNLVLQALLVLLKKAPPQGRKLLIIGTTSRKDVLQEMEMLNAFS-TTIHVPNIAT 667          
BLAST of Vesicle-fusing ATPase vs. Ensembl Human
Match: NSF (N-ethylmaleimide sensitive factor, vesicle fusing ATPase [Source:HGNC Symbol;Acc:HGNC:8016])

HSP 1 Score: 582.408 bits (1500), Expect = 0.000e+0
Identity = 292/661 (44.18%), Postives = 429/661 (64.90%), Query Frame = 2
Query:   35 DEFSYTNKIFFNPSDWALFKEKFITVTTASNKFIFKSDKDESVKKGCMTTNGIQRKWMYCGIGEVVECIPYSFQXXXXXXXXXXXXDLFVKSASL-KDPIDSDKLAVKYLERFHDTPVTKNQLLLFNWESINFEAKIISIQGLDVK-------SSKMNEIETGIIQSNTVFKFQRRPESKFMLSGRCKAENAAVCLINPNWDFNSIGVGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEKFDPKKGIAIRIEDLQSFKINRQHFIFAIDHDVKALYGRHDDDLQEFKENRLLKWSPQISKALSTAGLLIESLKTNDNISRKRLLIYGEPLTGTSSVAVHLAKETGFTFIRMCSMRNMIGFSEPARCLAIKKIFDDALKAPASVIILDDVERLIDYSSIGPRFSIFLVNNLIGMINCFVPKNKKMLIIGTTKHLSAMHELEFFSAFDKNCIKIPEIKT 1993
            DE S TN    N  D+    +  I  T+ ++++ F      SV  G +  +  QRKW    IG+ +E   Y+F         +     F++  S+  +P D+DK+A +++++F++   +  Q L+F++    F   +  I+ +D         + K  +IE G++  N+   F++   S   L G+ K +     +INP+W+F  +G+GGLD+EFS IF+ AF SR+FPPE VE++G KHV+GILLYGPPG GKTL ARQIGKMLNAREPK+VNGP IL+KYVGESEANIRKLFADAEEE +++G  S LHIIIFDEIDAICK RG+++G + V DTVVNQLL+K+DGVEQLNNIL+IGMTNR D+IDEALLRPGR+ V++E+ LP + GRLQI  IHT ++R   +L+ D+D+KELA  TKN+SGAE+E +V AA + A +        + + +E  +S ++ R  F+ ++++D+K  +G + +D   +  N ++KW   +++ L    LL++  K +D      +L+ G P +G +++A  +A+E+ F FI++CS   MIGFSE A+C A+KKIFDDA K+  S +++DD+ERL+DY  IGPRFS  ++  L+ ++    P+ +K+LIIGTT     + E+E  +AF    I +P I T
Sbjct:    9 DELSLTNCAVVNEKDFQ-SGQHVIVRTSPNHRYTFTLKTHPSVVPGSIAFSLPQRKWAGLSIGQEIEVSLYTFDKAKQCIGTMTIEIDFLQKKSIDSNPYDTDKMAAEFIQQFNNQAFSVGQQLVFSFNEKLFGLLVKDIEAMDPSILKGEPATGKRQKIEVGLVVGNSQVAFEKAENSSLNLIGKAKTKENRQSIINPDWNFEKMGIGGLDKEFSDIFRRAFASRVFPPEIVEQMGCKHVKGILLYGPPGCGKTLLARQIGKMLNAREPKVVNGPEILNKYVGESEANIRKLFADAEEEQRRLGANSGLHIIIFDEIDAICKQRGSMAGSTGVHDTVVNQLLSKIDGVEQLNNILVIGMTNRPDLIDEALLRPGRLEVKMEIGLPDEKGRLQILHIHTARMRGHQLLSADVDIKELAVETKNFSGAELEGLVRAAQSTAMNRHIKASTKVEVDMEKAESLQVTRGDFLASLENDIKPAFGTNQEDYASYIMNGIIKWGDPVTRVLDDGELLVQQTKNSDRTPLVSVLLEGPPHSGKTALAAKIAEESNFPFIKICSPDKMIGFSETAKCQAMKKIFDDAYKSQLSCVVVDDIERLLDYVPIGPRFSNLVLQALLVLLKKAPPQGRKLLIIGTTSRKDVLQEMEMLNAFS-TTIHVPNIAT 667          
BLAST of Vesicle-fusing ATPase vs. Ensembl Human
Match: NSF (N-ethylmaleimide sensitive factor, vesicle fusing ATPase [Source:HGNC Symbol;Acc:HGNC:8016])

HSP 1 Score: 582.408 bits (1500), Expect = 0.000e+0
Identity = 292/661 (44.18%), Postives = 429/661 (64.90%), Query Frame = 2
Query:   35 DEFSYTNKIFFNPSDWALFKEKFITVTTASNKFIFKSDKDESVKKGCMTTNGIQRKWMYCGIGEVVECIPYSFQXXXXXXXXXXXXDLFVKSASL-KDPIDSDKLAVKYLERFHDTPVTKNQLLLFNWESINFEAKIISIQGLDVK-------SSKMNEIETGIIQSNTVFKFQRRPESKFMLSGRCKAENAAVCLINPNWDFNSIGVGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEKFDPKKGIAIRIEDLQSFKINRQHFIFAIDHDVKALYGRHDDDLQEFKENRLLKWSPQISKALSTAGLLIESLKTNDNISRKRLLIYGEPLTGTSSVAVHLAKETGFTFIRMCSMRNMIGFSEPARCLAIKKIFDDALKAPASVIILDDVERLIDYSSIGPRFSIFLVNNLIGMINCFVPKNKKMLIIGTTKHLSAMHELEFFSAFDKNCIKIPEIKT 1993
            DE S TN    N  D+    +  I  T+ ++++ F      SV  G +  +  QRKW    IG+ +E   Y+F         +     F++  S+  +P D+DK+A +++++F++   +  Q L+F++    F   +  I+ +D         + K  +IE G++  N+   F++   S   L G+ K +     +INP+W+F  +G+GGLD+EFS IF+ AF SR+FPPE VE++G KHV+GILLYGPPG GKTL ARQIGKMLNAREPK+VNGP IL+KYVGESEANIRKLFADAEEE +++G  S LHIIIFDEIDAICK RG+++G + V DTVVNQLL+K+DGVEQLNNIL+IGMTNR D+IDEALLRPGR+ V++E+ LP + GRLQI  IHT ++R   +L+ D+D+KELA  TKN+SGAE+E +V AA + A +        + + +E  +S ++ R  F+ ++++D+K  +G + +D   +  N ++KW   +++ L    LL++  K +D      +L+ G P +G +++A  +A+E+ F FI++CS   MIGFSE A+C A+KKIFDDA K+  S +++DD+ERL+DY  IGPRFS  ++  L+ ++    P+ +K+LIIGTT     + E+E  +AF    I +P I T
Sbjct:    9 DELSLTNCAVVNEKDFQ-SGQHVIVRTSPNHRYTFTLKTHPSVVPGSIAFSLPQRKWAGLSIGQEIEVSLYTFDKAKQCIGTMTIEIDFLQKKSIDSNPYDTDKMAAEFIQQFNNQAFSVGQQLVFSFNEKLFGLLVKDIEAMDPSILKGEPATGKRQKIEVGLVVGNSQVAFEKAENSSLNLIGKAKTKENRQSIINPDWNFEKMGIGGLDKEFSDIFRRAFASRVFPPEIVEQMGCKHVKGILLYGPPGCGKTLLARQIGKMLNAREPKVVNGPEILNKYVGESEANIRKLFADAEEEQRRLGANSGLHIIIFDEIDAICKQRGSMAGSTGVHDTVVNQLLSKIDGVEQLNNILVIGMTNRPDLIDEALLRPGRLEVKMEIGLPDEKGRLQILHIHTARMRGHQLLSADVDIKELAVETKNFSGAELEGLVRAAQSTAMNRHIKASTKVEVDMEKAESLQVTRGDFLASLENDIKPAFGTNQEDYASYIMNGIIKWGDPVTRVLDDGELLVQQTKNSDRTPLVSVLLEGPPHSGKTALAAKIAEESNFPFIKICSPDKMIGFSETAKCQAMKKIFDDAYKSQLSCVVVDDIERLLDYVPIGPRFSNLVLQALLVLLKKAPPQGRKLLIIGTTSRKDVLQEMEMLNAFS-TTIHVPNIAT 667          
BLAST of Vesicle-fusing ATPase vs. Ensembl Human
Match: NSF (N-ethylmaleimide sensitive factor, vesicle fusing ATPase [Source:HGNC Symbol;Acc:HGNC:8016])

HSP 1 Score: 582.022 bits (1499), Expect = 0.000e+0
Identity = 292/661 (44.18%), Postives = 429/661 (64.90%), Query Frame = 2
Query:   35 DEFSYTNKIFFNPSDWALFKEKFITVTTASNKFIFKSDKDESVKKGCMTTNGIQRKWMYCGIGEVVECIPYSFQXXXXXXXXXXXXDLFVKSASL-KDPIDSDKLAVKYLERFHDTPVTKNQLLLFNWESINFEAKIISIQGLDVK-------SSKMNEIETGIIQSNTVFKFQRRPESKFMLSGRCKAENAAVCLINPNWDFNSIGVGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEKFDPKKGIAIRIEDLQSFKINRQHFIFAIDHDVKALYGRHDDDLQEFKENRLLKWSPQISKALSTAGLLIESLKTNDNISRKRLLIYGEPLTGTSSVAVHLAKETGFTFIRMCSMRNMIGFSEPARCLAIKKIFDDALKAPASVIILDDVERLIDYSSIGPRFSIFLVNNLIGMINCFVPKNKKMLIIGTTKHLSAMHELEFFSAFDKNCIKIPEIKT 1993
            DE S TN    N  D+    +  I  T+ ++++ F      SV  G +  +  QRKW    IG+ +E   Y+F         +     F++  S+  +P D+DK+A +++++F++   +  Q L+F++    F   +  I+ +D         + K  +IE G++  N+   F++   S   L G+ K +     +INP+W+F  +G+GGLD+EFS IF+ AF SR+FPPE VE++G KHV+GILLYGPPG GKTL ARQIGKMLNAREPK+VNGP IL+KYVGESEANIRKLFADAEEE +++G  S LHIIIFDEIDAICK RG+++G + V DTVVNQLL+K+DGVEQLNNIL+IGMTNR D+IDEALLRPGR+ V++E+ LP + GRLQI  IHT ++R   +L+ D+D+KELA  TKN+SGAE+E +V AA + A +        + + +E  +S ++ R  F+ ++++D+K  +G + +D   +  N ++KW   +++ L    LL++  K +D      +L+ G P +G +++A  +A+E+ F FI++CS   MIGFSE A+C A+KKIFDDA K+  S +++DD+ERL+DY  IGPRFS  ++  L+ ++    P+ +K+LIIGTT     + E+E  +AF    I +P I T
Sbjct:   14 DELSLTNCAVVNEKDFQ-SGQHVIVRTSPNHRYTFTLKTHPSVVPGSIAFSLPQRKWAGLSIGQEIEVSLYTFDKAKQCIGTMTIEIDFLQKKSIDSNPYDTDKMAAEFIQQFNNQAFSVGQQLVFSFNEKLFGLLVKDIEAMDPSILKGEPATGKRQKIEVGLVVGNSQVAFEKAENSSLNLIGKAKTKENRQSIINPDWNFEKMGIGGLDKEFSDIFRRAFASRVFPPEIVEQMGCKHVKGILLYGPPGCGKTLLARQIGKMLNAREPKVVNGPEILNKYVGESEANIRKLFADAEEEQRRLGANSGLHIIIFDEIDAICKQRGSMAGSTGVHDTVVNQLLSKIDGVEQLNNILVIGMTNRPDLIDEALLRPGRLEVKMEIGLPDEKGRLQILHIHTARMRGHQLLSADVDIKELAVETKNFSGAELEGLVRAAQSTAMNRHIKASTKVEVDMEKAESLQVTRGDFLASLENDIKPAFGTNQEDYASYIMNGIIKWGDPVTRVLDDGELLVQQTKNSDRTPLVSVLLEGPPHSGKTALAAKIAEESNFPFIKICSPDKMIGFSETAKCQAMKKIFDDAYKSQLSCVVVDDIERLLDYVPIGPRFSNLVLQALLVLLKKAPPQGRKLLIIGTTSRKDVLQEMEMLNAFS-TTIHVPNIAT 672          
BLAST of Vesicle-fusing ATPase vs. Ensembl Human
Match: NSF (N-ethylmaleimide sensitive factor, vesicle fusing ATPase [Source:HGNC Symbol;Acc:HGNC:8016])

HSP 1 Score: 582.022 bits (1499), Expect = 0.000e+0
Identity = 292/661 (44.18%), Postives = 429/661 (64.90%), Query Frame = 2
Query:   35 DEFSYTNKIFFNPSDWALFKEKFITVTTASNKFIFKSDKDESVKKGCMTTNGIQRKWMYCGIGEVVECIPYSFQXXXXXXXXXXXXDLFVKSASL-KDPIDSDKLAVKYLERFHDTPVTKNQLLLFNWESINFEAKIISIQGLDVK-------SSKMNEIETGIIQSNTVFKFQRRPESKFMLSGRCKAENAAVCLINPNWDFNSIGVGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEKFDPKKGIAIRIEDLQSFKINRQHFIFAIDHDVKALYGRHDDDLQEFKENRLLKWSPQISKALSTAGLLIESLKTNDNISRKRLLIYGEPLTGTSSVAVHLAKETGFTFIRMCSMRNMIGFSEPARCLAIKKIFDDALKAPASVIILDDVERLIDYSSIGPRFSIFLVNNLIGMINCFVPKNKKMLIIGTTKHLSAMHELEFFSAFDKNCIKIPEIKT 1993
            DE S TN    N  D+    +  I  T+ ++++ F      SV  G +  +  QRKW    IG+ +E   Y+F         +     F++  S+  +P D+DK+A +++++F++   +  Q L+F++    F   +  I+ +D         + K  +IE G++  N+   F++   S   L G+ K +     +INP+W+F  +G+GGLD+EFS IF+ AF SR+FPPE VE++G KHV+GILLYGPPG GKTL ARQIGKMLNAREPK+VNGP IL+KYVGESEANIRKLFADAEEE +++G  S LHIIIFDEIDAICK RG+++G + V DTVVNQLL+K+DGVEQLNNIL+IGMTNR D+IDEALLRPGR+ V++E+ LP + GRLQI  IHT ++R   +L+ D+D+KELA  TKN+SGAE+E +V AA + A +        + + +E  +S ++ R  F+ ++++D+K  +G + +D   +  N ++KW   +++ L    LL++  K +D      +L+ G P +G +++A  +A+E+ F FI++CS   MIGFSE A+C A+KKIFDDA K+  S +++DD+ERL+DY  IGPRFS  ++  L+ ++    P+ +K+LIIGTT     + E+E  +AF    I +P I T
Sbjct:   14 DELSLTNCAVVNEKDFQ-SGQHVIVRTSPNHRYTFTLKTHPSVVPGSIAFSLPQRKWAGLSIGQEIEVSLYTFDKAKQCIGTMTIEIDFLQKKSIDSNPYDTDKMAAEFIQQFNNQAFSVGQQLVFSFNEKLFGLLVKDIEAMDPSILKGEPATGKRQKIEVGLVVGNSQVAFEKAENSSLNLIGKAKTKENRQSIINPDWNFEKMGIGGLDKEFSDIFRRAFASRVFPPEIVEQMGCKHVKGILLYGPPGCGKTLLARQIGKMLNAREPKVVNGPEILNKYVGESEANIRKLFADAEEEQRRLGANSGLHIIIFDEIDAICKQRGSMAGSTGVHDTVVNQLLSKIDGVEQLNNILVIGMTNRPDLIDEALLRPGRLEVKMEIGLPDEKGRLQILHIHTARMRGHQLLSADVDIKELAVETKNFSGAELEGLVRAAQSTAMNRHIKASTKVEVDMEKAESLQVTRGDFLASLENDIKPAFGTNQEDYASYIMNGIIKWGDPVTRVLDDGELLVQQTKNSDRTPLVSVLLEGPPHSGKTALAAKIAEESNFPFIKICSPDKMIGFSETAKCQAMKKIFDDAYKSQLSCVVVDDIERLLDYVPIGPRFSNLVLQALLVLLKKAPPQGRKLLIIGTTSRKDVLQEMEMLNAFS-TTIHVPNIAT 672          
BLAST of Vesicle-fusing ATPase vs. Ensembl Celegans
Match: nsf-1 (Vesicle-fusing ATPase [Source:UniProtKB/Swiss-Prot;Acc:Q94392])

HSP 1 Score: 576.244 bits (1484), Expect = 0.000e+0
Identity = 291/690 (42.17%), Postives = 437/690 (63.33%), Query Frame = 2
Query:   11 FKVQKNSYDEFSYTNKIFFNPSDWALFKEKFITVTTA-SNKFIFKSDKDESVKKGCMTTNGIQRKWMYCGIGEVVECIPYSFQXXXXXXXXXXXXDLFVKSASLKDPIDSDKLAVKYLERFHDTPVTKNQLLLFNWE------SINFEAKIISIQGLDV---------------KSSKMNEIETGIIQSNTVFKFQRRPESKFMLSGRCKAENAAVCLINPNWDFNSIGVGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEKFDPKKGIAIRIEDLQSFKINRQHFIFAIDHDVKALYGRHDDDLQEFKENRLLKWSPQISKALSTAGLLIESLKTNDNISRKRLLIYGEPLTGTSSVAVHLAKETGFTFIRMCSMRNMIGFSEPARCLAIKKIFDDALKAPASVIILDDVERLIDYSSIGPRFSIFLVNNLIGMINCFVPKNKKMLIIGTTKHLSAMHELEFFSAFDKNCIKIPEIKTPSEIISV 2014
            F+V+K   +E +  N  + N SD+   + K + V    ++ +IF    D S+K G +      RKW    + + V   P++FQ    V  +I+  D   K     +P+++D +A ++  +F     +K   + F +E      +      + SI+G D+                ++K  +IE G +  N+V  F +   S   L G+ K ++A   +INP+WDF  +G+GGLD EFS IF+ AF SR+FPPEF+E+LG+KHVRGILL+GPPGTGKTL ARQIGKMLNAREPKIVNGP ILDKYVGESE+N+RKLFADAEEE+++ G  S LHIIIFDEIDAICK RG+++G S+V DTVVNQLL+KMDGVEQLNNIL+IGMTNRRDMIDEALLRPGR+ VQ+EV LP + GRLQI +IHT ++RE N +  ++DL++++  TKN+SGAE+E +V AA ++A +             + ++   IN   F +A+++D+K  +GR D+ L  F    ++ W P+++K L    LL  ++K  +N   + +++ G   TG +S+A  +AK + F F+++ S  + +GFSE A+C+A+KK F+DA ++  SV+++D++ERLIDY  +GPR+S  ++  L+ ++N   P   ++ +I T+     + ++     F  + I IP++ T  ++++V
Sbjct:    2 FRVRKAPSEEHTLANYAYVNRSDFDDKQIKHVRVNPGPAHHYIFSIRNDGSIKPGEIAFGVPHRKWAALSLDQEVRVTPFTFQQSEYVGSMILTADFNAKKNVTSEPLNADLMAREFSIQFGGQAFSKGMQMAFRFEDKEKNKTHTLSLVVKSIEGFDIGKAAAAASGASNTDSSATKPKQIEAGELLPNSVIVFDKEEGSMLNLIGKSKGKSAYRSIINPDWDFQQMGIGGLDTEFSHIFRRAFASRVFPPEFIEQLGMKHVRGILLFGPPGTGKTLMARQIGKMLNAREPKIVNGPQILDKYVGESESNVRKLFADAEEEWRRCGANSGLHIIIFDEIDAICKQRGSMAGSSSVHDTVVNQLLSKMDGVEQLNNILVIGMTNRRDMIDEALLRPGRLEVQMEVSLPDETGRLQILKIHTARMREYNKMDPNVDLEDISKRTKNFSGAELEGLVRAAQSSAMNRLVKAGGKAQADPDAIEKLAINSGDFDYALENDIKPAFGRSDESLNRFLSRGMIVWGPEVTKILDEGSLLAATVKNPENSGFRTVVLAGAAKTGKTSLAAQMAKSSDFPFVKVISPEDTVGFSESAKCMALKKAFEDAKRSKLSVLLIDNLERLIDYHPVGPRYSNLVIQALLVLLNAPPPAGHRLFVIATSSDRMFLRDMGLMDVFG-DVIDIPKLTTAGQMMNV 690          
BLAST of Vesicle-fusing ATPase vs. Ensembl Celegans
Match: nsf-1 (Vesicle-fusing ATPase [Source:UniProtKB/Swiss-Prot;Acc:Q94392])

HSP 1 Score: 576.244 bits (1484), Expect = 0.000e+0
Identity = 291/690 (42.17%), Postives = 437/690 (63.33%), Query Frame = 2
Query:   11 FKVQKNSYDEFSYTNKIFFNPSDWALFKEKFITVTTA-SNKFIFKSDKDESVKKGCMTTNGIQRKWMYCGIGEVVECIPYSFQXXXXXXXXXXXXDLFVKSASLKDPIDSDKLAVKYLERFHDTPVTKNQLLLFNWE------SINFEAKIISIQGLDV---------------KSSKMNEIETGIIQSNTVFKFQRRPESKFMLSGRCKAENAAVCLINPNWDFNSIGVGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEKFDPKKGIAIRIEDLQSFKINRQHFIFAIDHDVKALYGRHDDDLQEFKENRLLKWSPQISKALSTAGLLIESLKTNDNISRKRLLIYGEPLTGTSSVAVHLAKETGFTFIRMCSMRNMIGFSEPARCLAIKKIFDDALKAPASVIILDDVERLIDYSSIGPRFSIFLVNNLIGMINCFVPKNKKMLIIGTTKHLSAMHELEFFSAFDKNCIKIPEIKTPSEIISV 2014
            F+V+K   +E +  N  + N SD+   + K + V    ++ +IF    D S+K G +      RKW    + + V   P++FQ    V  +I+  D   K     +P+++D +A ++  +F     +K   + F +E      +      + SI+G D+                ++K  +IE G +  N+V  F +   S   L G+ K ++A   +INP+WDF  +G+GGLD EFS IF+ AF SR+FPPEF+E+LG+KHVRGILL+GPPGTGKTL ARQIGKMLNAREPKIVNGP ILDKYVGESE+N+RKLFADAEEE+++ G  S LHIIIFDEIDAICK RG+++G S+V DTVVNQLL+KMDGVEQLNNIL+IGMTNRRDMIDEALLRPGR+ VQ+EV LP + GRLQI +IHT ++RE N +  ++DL++++  TKN+SGAE+E +V AA ++A +             + ++   IN   F +A+++D+K  +GR D+ L  F    ++ W P+++K L    LL  ++K  +N   + +++ G   TG +S+A  +AK + F F+++ S  + +GFSE A+C+A+KK F+DA ++  SV+++D++ERLIDY  +GPR+S  ++  L+ ++N   P   ++ +I T+     + ++     F  + I IP++ T  ++++V
Sbjct:    2 FRVRKAPSEEHTLANYAYVNRSDFDDKQIKHVRVNPGPAHHYIFSIRNDGSIKPGEIAFGVPHRKWAALSLDQEVRVTPFTFQQSEYVGSMILTADFNAKKNVTSEPLNADLMAREFSIQFGGQAFSKGMQMAFRFEDKEKNKTHTLSLVVKSIEGFDIGKAAAAASGASNTDSSATKPKQIEAGELLPNSVIVFDKEEGSMLNLIGKSKGKSAYRSIINPDWDFQQMGIGGLDTEFSHIFRRAFASRVFPPEFIEQLGMKHVRGILLFGPPGTGKTLMARQIGKMLNAREPKIVNGPQILDKYVGESESNVRKLFADAEEEWRRCGANSGLHIIIFDEIDAICKQRGSMAGSSSVHDTVVNQLLSKMDGVEQLNNILVIGMTNRRDMIDEALLRPGRLEVQMEVSLPDETGRLQILKIHTARMREYNKMDPNVDLEDISKRTKNFSGAELEGLVRAAQSSAMNRLVKAGGKAQADPDAIEKLAINSGDFDYALENDIKPAFGRSDESLNRFLSRGMIVWGPEVTKILDEGSLLAATVKNPENSGFRTVVLAGAAKTGKTSLAAQMAKSSDFPFVKVISPEDTVGFSESAKCMALKKAFEDAKRSKLSVLLIDNLERLIDYHPVGPRYSNLVIQALLVLLNAPPPAGHRLFVIATSSDRMFLRDMGLMDVFG-DVIDIPKLTTAGQMMNV 690          
BLAST of Vesicle-fusing ATPase vs. Ensembl Celegans
Match: nsf-1 (Vesicle-fusing ATPase [Source:UniProtKB/Swiss-Prot;Acc:Q94392])

HSP 1 Score: 575.474 bits (1482), Expect = 0.000e+0
Identity = 291/690 (42.17%), Postives = 437/690 (63.33%), Query Frame = 2
Query:   11 FKVQKNSYDEFSYTNKIFFNPSDWALFKEKFITVTTA-SNKFIFKSDKDESVKKGCMTTNGIQRKWMYCGIGEVVECIPYSFQXXXXXXXXXXXXDLFVKSASLKDPIDSDKLAVKYLERFHDTPVTKNQLLLFNWE------SINFEAKIISIQGLDV---------------KSSKMNEIETGIIQSNTVFKFQRRPESKFMLSGRCKAENAAVCLINPNWDFNSIGVGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEKFDPKKGIAIRIEDLQSFKINRQHFIFAIDHDVKALYGRHDDDLQEFKENRLLKWSPQISKALSTAGLLIESLKTNDNISRKRLLIYGEPLTGTSSVAVHLAKETGFTFIRMCSMRNMIGFSEPARCLAIKKIFDDALKAPASVIILDDVERLIDYSSIGPRFSIFLVNNLIGMINCFVPKNKKMLIIGTTKHLSAMHELEFFSAFDKNCIKIPEIKTPSEIISV 2014
            F+V+K   +E +  N  + N SD+   + K + V    ++ +IF    D S+K G +      RKW    + + V   P++FQ    V  +I+  D   K     +P+++D +A ++  +F     +K   + F +E      +      + SI+G D+                ++K  +IE G +  N+V  F +   S   L G+ K ++A   +INP+WDF  +G+GGLD EFS IF+ AF SR+FPPEF+E+LG+KHVRGILL+GPPGTGKTL ARQIGKMLNAREPKIVNGP ILDKYVGESE+N+RKLFADAEEE+++ G  S LHIIIFDEIDAICK RG+++G S+V DTVVNQLL+KMDGVEQLNNIL+IGMTNRRDMIDEALLRPGR+ VQ+EV LP + GRLQI +IHT ++RE N +  ++DL++++  TKN+SGAE+E +V AA ++A +             + ++   IN   F +A+++D+K  +GR D+ L  F    ++ W P+++K L    LL  ++K  +N   + +++ G   TG +S+A  +AK + F F+++ S  + +GFSE A+C+A+KK F+DA ++  SV+++D++ERLIDY  +GPR+S  ++  L+ ++N   P   ++ +I T+     + ++     F  + I IP++ T  ++++V
Sbjct:   68 FRVRKAPSEEHTLANYAYVNRSDFDDKQIKHVRVNPGPAHHYIFSIRNDGSIKPGEIAFGVPHRKWAALSLDQEVRVTPFTFQQSEYVGSMILTADFNAKKNVTSEPLNADLMAREFSIQFGGQAFSKGMQMAFRFEDKEKNKTHTLSLVVKSIEGFDIGKAAAAASGASNTDSSATKPKQIEAGELLPNSVIVFDKEEGSMLNLIGKSKGKSAYRSIINPDWDFQQMGIGGLDTEFSHIFRRAFASRVFPPEFIEQLGMKHVRGILLFGPPGTGKTLMARQIGKMLNAREPKIVNGPQILDKYVGESESNVRKLFADAEEEWRRCGANSGLHIIIFDEIDAICKQRGSMAGSSSVHDTVVNQLLSKMDGVEQLNNILVIGMTNRRDMIDEALLRPGRLEVQMEVSLPDETGRLQILKIHTARMREYNKMDPNVDLEDISKRTKNFSGAELEGLVRAAQSSAMNRLVKAGGKAQADPDAIEKLAINSGDFDYALENDIKPAFGRSDESLNRFLSRGMIVWGPEVTKILDEGSLLAATVKNPENSGFRTVVLAGAAKTGKTSLAAQMAKSSDFPFVKVISPEDTVGFSESAKCMALKKAFEDAKRSKLSVLLIDNLERLIDYHPVGPRYSNLVIQALLVLLNAPPPAGHRLFVIATSSDRMFLRDMGLMDVFG-DVIDIPKLTTAGQMMNV 756          
BLAST of Vesicle-fusing ATPase vs. Ensembl Celegans
Match: cdc-48.1 (Transitional endoplasmic reticulum ATPase homolog 1 [Source:UniProtKB/Swiss-Prot;Acc:P54811])

HSP 1 Score: 155.606 bits (392), Expect = 5.564e-39
Identity = 110/309 (35.60%), Postives = 166/309 (53.72%), Query Frame = 2
Query:  572 KAENAAVCLINPNW----------------DFNSIG---VGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPK----IVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEKFD--PKKGIAIRIEDLQSFKINRQHFIFA 1423
            + E A  C+++P+                   N IG   +GG+ ++ +QI +   L  L  P+  + +GIK  RGILL+GPPGTGKTL AR +     A E      ++NGP ++ K  GESE+N+RK F + E+      NQ A  I+  DEIDAI   R   +G   V+  +V+QLL  MDGV+  +N+++I  TNR + ID AL R GR   +I++ +P   GRL+I  IHTK ++    LADD+DL+++A     + GA++ ++   A      EK +    +   I  E L S  +  ++F FA
Sbjct:  173 ETEPAPACIVSPDTMIHYEGDPIKREEEEESMNDIGYDDLGGVRKQLAQIKEMVELP-LRHPQLFKAIGIKPPRGILLFGPPGTGKTLIARAV-----ANETGSFFFLINGPEVMSKMSGESESNLRKAFEECEK------NQPA--ILFIDEIDAIAPKREKTNG--EVERRIVSQLLTLMDGVKGRSNLVVIAATNRPNSIDGALRRFGRFDREIDIGIPDAVGRLEILRIHTKNMK----LADDVDLEQIANECHGFVGADLASLCSEAALQQIREKMELIDLEDDQIDAEVLNSLAVTMENFRFA 461          

HSP 2 Score: 130.183 bits (326), Expect = 7.667e-31
Identity = 92/229 (40.17%), Postives = 127/229 (55.46%), Query Frame = 2
Query:  698 PEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAV--QDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEKFDPKKGIAIRIE 1378
            PE   + G++  RG+L YGPPG GKTL A+ I     A    I  GP +L  + GESEAN+R +F  A          +A  ++ FDE+D+I KARG  +GG      D V+NQ+L +MDG+    N+ IIG TNR D+ID A+LRPGR+   I +PLP +  R QI +   +    K  L+ D+DL  LA  T  +SGA++  +   A   A  E  + +    IRIE
Sbjct:  506 PEKYLKFGMQPSRGVLFYGPPGCGKTLLAKAIANECQANFISI-KGPELLTMWFGESEANVRDVFDKA--------RAAAPCVLFFDELDSIAKARGGGAGGDGGGASDRVINQVLTEMDGMNAKKNVFIIGATNRPDIIDPAVLRPGRLDQLIYIPLPDEASRHQILKASLR----KTPLSKDLDLTFLAKNTVGFSGADLTEICQRACKLAIRESIEKE----IRIE 717          
BLAST of Vesicle-fusing ATPase vs. Ensembl Celegans
Match: cdc-48.3 (ATPase family protein 2 homolog [Source:UniProtKB/Swiss-Prot;Acc:Q21222])

HSP 1 Score: 150.214 bits (378), Expect = 2.192e-37
Identity = 105/285 (36.84%), Postives = 146/285 (51.23%), Query Frame = 2
Query:  605 PNWDFNSIGVGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKI----VNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEKFDPKKGIAIRIEDLQSFKINR-QHFIFAIDHDVKA 1444
            PN  +N IG    +EE     + A +     PE  E  GI    GILLYGPPG  KTL AR +     A E K+    V GP +  K+VG+SE  IR LF+ A         Q A  I+ FDEIDA+  +RG+    S V D V+ QLL ++DG+E+ + ++++  TNR D +D ALLRPGR+   I V LP +  R  I E+ TKK++  + +     + +L   T  YSGAE+ AV   A   A  E  D         E   +  ++R + ++  I  D KA
Sbjct:  454 PNVSWNDIGG---NEELKLEIQQAVIWPQKHPEAFERFGIDPPAGILLYGPPGCSKTLIARAL-----ASEAKMNFLAVKGPELFSKWVGDSEKAIRDLFSRA--------RQVAPTIVFFDEIDAVGSSRGS-EKSSGVSDRVLAQLLTELDGLEKSSRVILLAATNRPDQLDSALLRPGRLDRAIYVGLPCEVTRRAILEMRTKKMKFDDTVR---TIDKLVEKTSGYSGAELVAVCRTAAMFAMRESIDATIVQWTHFEQALAAVVSRTEAYLLEIYDDFKA 718          
BLAST of Vesicle-fusing ATPase vs. Ensembl Fly
Match: comt (gene:FBgn0000346 transcript:FBtr0073754)

HSP 1 Score: 597.43 bits (1539), Expect = 0.000e+0
Identity = 305/679 (44.92%), Postives = 437/679 (64.36%), Query Frame = 2
Query:   11 FKVQKNSYDEFSYTNKIFFNPSDWALFKE--KFITVTTASNK-FIFKSDKDESVKKGCMTTNGIQRKWMYCGIGEVVECIPYSFQXXXXXXX-XXXXXDLFVKSASLKDPIDSDKLAVKYLERFHDTPVTKNQLLLFNWESINFEA-KIISIQGLDVKS------SKMNEIETGIIQSNTVFKFQRRPESKFMLSGRCKAENAAVCLINPNWDFNSIGVGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEKFDPKKGIAIRIEDLQSFKINRQHFIFAIDHDVKALYGRHDDDLQEFKENRLLKWSPQISKALSTAGLLIESLKTNDNISRKRLLIYGEPLTGTSSVAVHLAKETGFTFIRMCSMRNMIGFSEPARCLAIKKIFDDALKAPASVIILDDVERLIDYSSIGPRFSIFLVNNLIGMINCFVPKNKKMLIIGTTKHLSAMHELEFFSAFDKNCIKIPEIKTPSEIISV 2014
             K  K   DE S TN+   N  D   F E  K+  ++ A  + FIF  +K   V  G +  + +QRKW    I + +E  PY F     V   V  E D   K    ++P DSD++A +++ +F    +T  Q L+FN++        + S++ +D KS      + M  +  G I  NTV +F++   S   L G+ K +     +INP+WDF  +G+GGLD+EF+ IF+ AF SR+FPPE VE+LG KHV+GILLYGPPGTGKTL ARQIG MLNAREPKIVNGP ILDKYVGESEAN+R+LFA+AEEE K++G  S LHIIIFDEIDAICK RG+++G S V DTVVNQLL K+DGV+QLNNIL+IGMTNRRDMIDEALLRPGR+ VQ+E+ LP++ GR+QI  IHTK++RE N + DD+D KE+AALTKN+SGAE+E +V AA ++A +        + +  E ++  K+NR  F+ +++HD+K  +G   + L       ++ W   +S  L    L ++  K  ++     +L+ G P +G +++A  LAK + F F+++CS  +M+G++E A+CL I+KIFDDA ++  S I++D+VERL+DY SIGPR+S   +  L+ ++    PK +K+LI+ T+     + E+E  +AF  + + +P +  P  +++V
Sbjct:    5 LKATKCPTDELSLTNRAIVNVGD---FPEEIKYADISPAPGQHFIFALEKTVEVPSGYVGFSLVQRKWAMVSINQELEVRPYRFDASSDVITCVSFETDFLQKKTVSQEPYDSDQMAKEFIMQFAGMALTVGQSLVFNFKDKKLLGLAVKSLEAIDPKSLGEGKDTAMRNVRFGRILGNTVVQFEKAENSSLNLQGKSKGKVVRQSIINPDWDFGKMGIGGLDKEFNSIFRRAFASRVFPPELVEQLGCKHVKGILLYGPPGTGKTLMARQIGTMLNAREPKIVNGPQILDKYVGESEANVRRLFAEAEEEEKRLGPNSGLHIIIFDEIDAICKQRGSVAGNSGVHDTVVNQLLTKIDGVDQLNNILVIGMTNRRDMIDEALLRPGRLEVQMEISLPNEQGRVQILNIHTKRMREFNKINDDVDNKEIAALTKNFSGAELEGLVRAAQSSAMNRLIKADAKVTVDPEAMEKLKVNRDDFLHSLEHDIKPAFGTAQEILDNMLARGVINWGAPVSNLLEDGMLYVQQAKAPESSGLVSVLVAGAPNSGKTALAAQLAKMSDFPFVKVCSPEDMVGYTESAKCLHIRKIFDDAYRSMLSCIVVDNVERLLDYGSIGPRYSNMTLQALLVLLKKQPPKGRKLLILCTSSRREVLEEMEMLTAF-TSVLHVPNLSKPDHVLAV 679          
BLAST of Vesicle-fusing ATPase vs. Ensembl Fly
Match: comt (gene:FBgn0000346 transcript:FBtr0333329)

HSP 1 Score: 597.43 bits (1539), Expect = 0.000e+0
Identity = 305/679 (44.92%), Postives = 437/679 (64.36%), Query Frame = 2
Query:   11 FKVQKNSYDEFSYTNKIFFNPSDWALFKE--KFITVTTASNK-FIFKSDKDESVKKGCMTTNGIQRKWMYCGIGEVVECIPYSFQXXXXXXX-XXXXXDLFVKSASLKDPIDSDKLAVKYLERFHDTPVTKNQLLLFNWESINFEA-KIISIQGLDVKS------SKMNEIETGIIQSNTVFKFQRRPESKFMLSGRCKAENAAVCLINPNWDFNSIGVGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEKFDPKKGIAIRIEDLQSFKINRQHFIFAIDHDVKALYGRHDDDLQEFKENRLLKWSPQISKALSTAGLLIESLKTNDNISRKRLLIYGEPLTGTSSVAVHLAKETGFTFIRMCSMRNMIGFSEPARCLAIKKIFDDALKAPASVIILDDVERLIDYSSIGPRFSIFLVNNLIGMINCFVPKNKKMLIIGTTKHLSAMHELEFFSAFDKNCIKIPEIKTPSEIISV 2014
             K  K   DE S TN+   N  D   F E  K+  ++ A  + FIF  +K   V  G +  + +QRKW    I + +E  PY F     V   V  E D   K    ++P DSD++A +++ +F    +T  Q L+FN++        + S++ +D KS      + M  +  G I  NTV +F++   S   L G+ K +     +INP+WDF  +G+GGLD+EF+ IF+ AF SR+FPPE VE+LG KHV+GILLYGPPGTGKTL ARQIG MLNAREPKIVNGP ILDKYVGESEAN+R+LFA+AEEE K++G  S LHIIIFDEIDAICK RG+++G S V DTVVNQLL K+DGV+QLNNIL+IGMTNRRDMIDEALLRPGR+ VQ+E+ LP++ GR+QI  IHTK++RE N + DD+D KE+AALTKN+SGAE+E +V AA ++A +        + +  E ++  K+NR  F+ +++HD+K  +G   + L       ++ W   +S  L    L ++  K  ++     +L+ G P +G +++A  LAK + F F+++CS  +M+G++E A+CL I+KIFDDA ++  S I++D+VERL+DY SIGPR+S   +  L+ ++    PK +K+LI+ T+     + E+E  +AF  + + +P +  P  +++V
Sbjct:    5 LKATKCPTDELSLTNRAIVNVGD---FPEEIKYADISPAPGQHFIFALEKTVEVPSGYVGFSLVQRKWAMVSINQELEVRPYRFDASSDVITCVSFETDFLQKKTVSQEPYDSDQMAKEFIMQFAGMALTVGQSLVFNFKDKKLLGLAVKSLEAIDPKSLGEGKDTAMRNVRFGRILGNTVVQFEKAENSSLNLQGKSKGKVVRQSIINPDWDFGKMGIGGLDKEFNSIFRRAFASRVFPPELVEQLGCKHVKGILLYGPPGTGKTLMARQIGTMLNAREPKIVNGPQILDKYVGESEANVRRLFAEAEEEEKRLGPNSGLHIIIFDEIDAICKQRGSVAGNSGVHDTVVNQLLTKIDGVDQLNNILVIGMTNRRDMIDEALLRPGRLEVQMEISLPNEQGRVQILNIHTKRMREFNKINDDVDNKEIAALTKNFSGAELEGLVRAAQSSAMNRLIKADAKVTVDPEAMEKLKVNRDDFLHSLEHDIKPAFGTAQEILDNMLARGVINWGAPVSNLLEDGMLYVQQAKAPESSGLVSVLVAGAPNSGKTALAAQLAKMSDFPFVKVCSPEDMVGYTESAKCLHIRKIFDDAYRSMLSCIVVDNVERLLDYGSIGPRYSNMTLQALLVLLKKQPPKGRKLLILCTSSRREVLEEMEMLTAF-TSVLHVPNLSKPDHVLAV 679          
BLAST of Vesicle-fusing ATPase vs. Ensembl Fly
Match: Nsf2 (gene:FBgn0266464 transcript:FBtr0344766)

HSP 1 Score: 590.112 bits (1520), Expect = 0.000e+0
Identity = 320/739 (43.30%), Postives = 471/739 (63.73%), Query Frame = 2
Query:   23 KNSYDEFSYTNKIFFNPSDWALFKEKFITVTTASN-KFIFKSDK--DESVKKGCMTTNGIQRKWMYCGIGEVVECIPYSFQXXXXXXXXXX-XXDLFVKSASLKDPIDSDKLAVKYLERFHDTPVTKNQLLLFNWESINFEA-KIISIQGLDVKS-----SKMNEIETGIIQSNTVFKFQRRPESKFMLSGRCKAENAAVCLINPNWDFNSIGVGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEKFDPKKGIAIRIEDLQSFKINRQHFIFAIDHDVKALYGRHDDDLQEFKENRLLKWSPQISKALSTAGLLIESLKTNDNISRKRLLIYGEPLTGTSSVAVHLAKETGFTFIRMCSMRNMIGFSEPARCLAIKKIFDDALKAPASVIILDDVERLIDYSSIGPRFSIFLVNNLIGMINCFVPKNKKMLIIGTTKHLSAMHELEFFSAFDKNCIKIPEIKTPSEIISVXXXXXXXXXXXXXXXXNHLQMGDCSDLAIGIKNLMENCKDLKSVDPARRVDELLMKLKSSSEFQF 2209
            K   DE S TNK   N SD+   + K++ ++      +IF  +K     +  G +  + +QRKW    I + ++  PY F     +  ++  E D   K  + ++P DSD++A ++L +F   P+T  Q L+F ++   F    + +++ +D ++      K   +  G I  NTV +F++   S   L GR K +     +INP+WDF  +G+GGLD+EF+ IF+ AF SR+FPPE VE+LGIKHV+GILLYGPPGTGKTL ARQIG MLNAREPKIVNGP ILDKYVGESEANIR+LFA+AEEE K++G  S LHIIIFDEIDAICKARG+++G S V DTVVNQLLAK+DGVEQLNNIL+IGMTNRRDMIDEALLRPGR+ VQ+E+ LP++ GR+QI  IHTK++R+ N +A D+D  E+AA TKN+SGAE+E +V AA + A +        + +  E ++  ++ R  F+ A+D+D+K  +G   + L+      ++ W P +++ L    L ++  K  ++     +LI G P +G S++A +LA+ + F F+++CS  +M+GF+E A+CL I+KIFDDA ++  S I++D+VERL+DY  IGPR+S   +  L+ ++    PK +K+LI+ T+     + E+E  SAF  + + +  + TP  +++VL D DL+S  +LQ I   +       L IGIK L+     ++  +P +RV + L K++     + 
Sbjct:   13 KCPTDELSLTNKAIVNVSDFT-EEVKYVDISPGPGLHYIFALEKISGPELPLGHVGFSLVQRKWATLSINQEIDVRPYRFDASADIITLVSFETDFLQKKTTTQEPYDSDEMAKEFLMQFAGMPLTVGQTLVFQFKDKKFLGLAVKTLEAVDPRTVGDSLPKTRNVRFGRILGNTVVQFEKAENSVLNLQGRSKGKIVRQSIINPDWDFGKMGIGGLDKEFNAIFRRAFASRVFPPELVEQLGIKHVKGILLYGPPGTGKTLMARQIGTMLNAREPKIVNGPQILDKYVGESEANIRRLFAEAEEEEKRLGPNSGLHIIIFDEIDAICKARGSVAGNSGVHDTVVNQLLAKIDGVEQLNNILVIGMTNRRDMIDEALLRPGRLEVQMEISLPNEQGRVQILNIHTKRMRDFNKIASDVDNNEIAAKTKNFSGAELEGLVRAAQSTAMNRLIKADSKVHVDPEAMEKLRVTRADFLHALDNDIKPAFGAAQEMLENLLARGIINWGPPVTELLEDGMLSVQQAKATESSGLVSVLIEGAPNSGKSALAANLAQLSDFPFVKVCSPEDMVGFTESAKCLHIRKIFDDAYRSTLSCIVVDNVERLLDYGPIGPRYSNLTLQALLVLLKKQPPKGRKLLILCTSSRRDVLEEMEMLSAF-TSVLHVSNLSTPENVLAVLDDSDLFSPEELQSIARKMA---GKRLCIGIKKLLALIDMIRQSEPHQRVIKFLSKMEEEGGLEM 746          
BLAST of Vesicle-fusing ATPase vs. Ensembl Fly
Match: Nsf2 (gene:FBgn0266464 transcript:FBtr0344767)

HSP 1 Score: 590.112 bits (1520), Expect = 0.000e+0
Identity = 320/739 (43.30%), Postives = 471/739 (63.73%), Query Frame = 2
Query:   23 KNSYDEFSYTNKIFFNPSDWALFKEKFITVTTASN-KFIFKSDK--DESVKKGCMTTNGIQRKWMYCGIGEVVECIPYSFQXXXXXXXXXX-XXDLFVKSASLKDPIDSDKLAVKYLERFHDTPVTKNQLLLFNWESINFEA-KIISIQGLDVKS-----SKMNEIETGIIQSNTVFKFQRRPESKFMLSGRCKAENAAVCLINPNWDFNSIGVGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEKFDPKKGIAIRIEDLQSFKINRQHFIFAIDHDVKALYGRHDDDLQEFKENRLLKWSPQISKALSTAGLLIESLKTNDNISRKRLLIYGEPLTGTSSVAVHLAKETGFTFIRMCSMRNMIGFSEPARCLAIKKIFDDALKAPASVIILDDVERLIDYSSIGPRFSIFLVNNLIGMINCFVPKNKKMLIIGTTKHLSAMHELEFFSAFDKNCIKIPEIKTPSEIISVXXXXXXXXXXXXXXXXNHLQMGDCSDLAIGIKNLMENCKDLKSVDPARRVDELLMKLKSSSEFQF 2209
            K   DE S TNK   N SD+   + K++ ++      +IF  +K     +  G +  + +QRKW    I + ++  PY F     +  ++  E D   K  + ++P DSD++A ++L +F   P+T  Q L+F ++   F    + +++ +D ++      K   +  G I  NTV +F++   S   L GR K +     +INP+WDF  +G+GGLD+EF+ IF+ AF SR+FPPE VE+LGIKHV+GILLYGPPGTGKTL ARQIG MLNAREPKIVNGP ILDKYVGESEANIR+LFA+AEEE K++G  S LHIIIFDEIDAICKARG+++G S V DTVVNQLLAK+DGVEQLNNIL+IGMTNRRDMIDEALLRPGR+ VQ+E+ LP++ GR+QI  IHTK++R+ N +A D+D  E+AA TKN+SGAE+E +V AA + A +        + +  E ++  ++ R  F+ A+D+D+K  +G   + L+      ++ W P +++ L    L ++  K  ++     +LI G P +G S++A +LA+ + F F+++CS  +M+GF+E A+CL I+KIFDDA ++  S I++D+VERL+DY  IGPR+S   +  L+ ++    PK +K+LI+ T+     + E+E  SAF  + + +  + TP  +++VL D DL+S  +LQ I   +       L IGIK L+     ++  +P +RV + L K++     + 
Sbjct:   13 KCPTDELSLTNKAIVNVSDFT-EEVKYVDISPGPGLHYIFALEKISGPELPLGHVGFSLVQRKWATLSINQEIDVRPYRFDASADIITLVSFETDFLQKKTTTQEPYDSDEMAKEFLMQFAGMPLTVGQTLVFQFKDKKFLGLAVKTLEAVDPRTVGDSLPKTRNVRFGRILGNTVVQFEKAENSVLNLQGRSKGKIVRQSIINPDWDFGKMGIGGLDKEFNAIFRRAFASRVFPPELVEQLGIKHVKGILLYGPPGTGKTLMARQIGTMLNAREPKIVNGPQILDKYVGESEANIRRLFAEAEEEEKRLGPNSGLHIIIFDEIDAICKARGSVAGNSGVHDTVVNQLLAKIDGVEQLNNILVIGMTNRRDMIDEALLRPGRLEVQMEISLPNEQGRVQILNIHTKRMRDFNKIASDVDNNEIAAKTKNFSGAELEGLVRAAQSTAMNRLIKADSKVHVDPEAMEKLRVTRADFLHALDNDIKPAFGAAQEMLENLLARGIINWGPPVTELLEDGMLSVQQAKATESSGLVSVLIEGAPNSGKSALAANLAQLSDFPFVKVCSPEDMVGFTESAKCLHIRKIFDDAYRSTLSCIVVDNVERLLDYGPIGPRYSNLTLQALLVLLKKQPPKGRKLLILCTSSRRDVLEEMEMLSAF-TSVLHVSNLSTPENVLAVLDDSDLFSPEELQSIARKMA---GKRLCIGIKKLLALIDMIRQSEPHQRVIKFLSKMEEEGGLEM 746          
BLAST of Vesicle-fusing ATPase vs. Ensembl Fly
Match: Nsf2 (gene:FBgn0266464 transcript:FBtr0082883)

HSP 1 Score: 590.112 bits (1520), Expect = 0.000e+0
Identity = 320/739 (43.30%), Postives = 471/739 (63.73%), Query Frame = 2
Query:   23 KNSYDEFSYTNKIFFNPSDWALFKEKFITVTTASN-KFIFKSDK--DESVKKGCMTTNGIQRKWMYCGIGEVVECIPYSFQXXXXXXXXXX-XXDLFVKSASLKDPIDSDKLAVKYLERFHDTPVTKNQLLLFNWESINFEA-KIISIQGLDVKS-----SKMNEIETGIIQSNTVFKFQRRPESKFMLSGRCKAENAAVCLINPNWDFNSIGVGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEKFDPKKGIAIRIEDLQSFKINRQHFIFAIDHDVKALYGRHDDDLQEFKENRLLKWSPQISKALSTAGLLIESLKTNDNISRKRLLIYGEPLTGTSSVAVHLAKETGFTFIRMCSMRNMIGFSEPARCLAIKKIFDDALKAPASVIILDDVERLIDYSSIGPRFSIFLVNNLIGMINCFVPKNKKMLIIGTTKHLSAMHELEFFSAFDKNCIKIPEIKTPSEIISVXXXXXXXXXXXXXXXXNHLQMGDCSDLAIGIKNLMENCKDLKSVDPARRVDELLMKLKSSSEFQF 2209
            K   DE S TNK   N SD+   + K++ ++      +IF  +K     +  G +  + +QRKW    I + ++  PY F     +  ++  E D   K  + ++P DSD++A ++L +F   P+T  Q L+F ++   F    + +++ +D ++      K   +  G I  NTV +F++   S   L GR K +     +INP+WDF  +G+GGLD+EF+ IF+ AF SR+FPPE VE+LGIKHV+GILLYGPPGTGKTL ARQIG MLNAREPKIVNGP ILDKYVGESEANIR+LFA+AEEE K++G  S LHIIIFDEIDAICKARG+++G S V DTVVNQLLAK+DGVEQLNNIL+IGMTNRRDMIDEALLRPGR+ VQ+E+ LP++ GR+QI  IHTK++R+ N +A D+D  E+AA TKN+SGAE+E +V AA + A +        + +  E ++  ++ R  F+ A+D+D+K  +G   + L+      ++ W P +++ L    L ++  K  ++     +LI G P +G S++A +LA+ + F F+++CS  +M+GF+E A+CL I+KIFDDA ++  S I++D+VERL+DY  IGPR+S   +  L+ ++    PK +K+LI+ T+     + E+E  SAF  + + +  + TP  +++VL D DL+S  +LQ I   +       L IGIK L+     ++  +P +RV + L K++     + 
Sbjct:   13 KCPTDELSLTNKAIVNVSDFT-EEVKYVDISPGPGLHYIFALEKISGPELPLGHVGFSLVQRKWATLSINQEIDVRPYRFDASADIITLVSFETDFLQKKTTTQEPYDSDEMAKEFLMQFAGMPLTVGQTLVFQFKDKKFLGLAVKTLEAVDPRTVGDSLPKTRNVRFGRILGNTVVQFEKAENSVLNLQGRSKGKIVRQSIINPDWDFGKMGIGGLDKEFNAIFRRAFASRVFPPELVEQLGIKHVKGILLYGPPGTGKTLMARQIGTMLNAREPKIVNGPQILDKYVGESEANIRRLFAEAEEEEKRLGPNSGLHIIIFDEIDAICKARGSVAGNSGVHDTVVNQLLAKIDGVEQLNNILVIGMTNRRDMIDEALLRPGRLEVQMEISLPNEQGRVQILNIHTKRMRDFNKIASDVDNNEIAAKTKNFSGAELEGLVRAAQSTAMNRLIKADSKVHVDPEAMEKLRVTRADFLHALDNDIKPAFGAAQEMLENLLARGIINWGPPVTELLEDGMLSVQQAKATESSGLVSVLIEGAPNSGKSALAANLAQLSDFPFVKVCSPEDMVGFTESAKCLHIRKIFDDAYRSTLSCIVVDNVERLLDYGPIGPRYSNLTLQALLVLLKKQPPKGRKLLILCTSSRRDVLEEMEMLSAF-TSVLHVSNLSTPENVLAVLDDSDLFSPEELQSIARKMA---GKRLCIGIKKLLALIDMIRQSEPHQRVIKFLSKMEEEGGLEM 746          
BLAST of Vesicle-fusing ATPase vs. Ensembl Zebrafish
Match: nsfa (N-ethylmaleimide-sensitive factor a [Source:ZFIN;Acc:ZDB-GENE-030616-37])

HSP 1 Score: 575.859 bits (1483), Expect = 0.000e+0
Identity = 311/727 (42.78%), Postives = 457/727 (62.86%), Query Frame = 2
Query:   35 DEFSYTNKIFFNPSDWALFKEKFITV-TTASNKFIFKSDKDESVKKGCMTTNGIQRKWMYCGIGEVVECIPYSF-QXXXXXXXXXXXXDLFVKSASLKDPIDSDKLAVKYLERFHDTPVTKNQLLLFNWESINFEAKIISIQGLDVK-------SSKMNEIETGIIQSNTVFKFQRRPESKFMLSGRCKAENAAVCLINPNWDFNSIGVGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEKFDPKKGIAIRIEDLQSFKINRQHFIFAIDHDVKALYGRHDDDLQEFKENRLLKWSPQISKALSTAGLLIESLKTNDNISRKRLLIYGEPLTGTSSVAVHLAKETGFTFIRMCSMRNMIGFSEPARCLAIKKIFDDALKAPASVIILDDVERLIDYSSIGPRFSIFLVNNLIGMINCFVPKNKKMLIIGTTKHLSAMHELEFFSAFDKNCIKIPEIKTPSEIISVXXXXXXXXXXXXXXXXNHLQMGDCSDLAIGIKNLMENCKDLKSVDPARRVDELLMKLK 2188
            DE S TN    +  D  L   + +TV TT ++KF+F      SV  G +  +  QRKW    IG+ +E   Y+F ++   +  + +E+D   K ++   P DSDK+A ++++ F++   +  Q L+F++    F   I  I+ +D         S K  +IE G++  N+   F++   S   L G+ K + A   +INP+W+F  +G+GGLD+EFS IF+ AF SR+FPP+ VE++G KHV+GILL+GPPG GKTL ARQIGKMLNAREPKIVNGP IL+KYVGESEANIRKLFADAEEE K++G  S LHIIIFDE+DAICK RG  +  + V DTVVNQLL+K+DGVEQLNNIL+IGMTNR D+IDEAL+RPGR  V++E+ LP + GR+QI  IHT K+RE  +LA D+D+KELAA TKNYSGAE+E +V AA + A +        + + +E  +  ++ R  F+ ++++D+K  +G + +D   +  N ++KW   +++ L    LL++  K +D      +L+ G P +G +++A  +++++ F FI++CS   MIG SE ++C AIKKIFDDA K+  S +++DD+ERL+DY  IGPRFS  ++  L+ ++    P  +K+LIIGTT     + E+E   AF    I +  I +   ++  L     ++D++   I  H++      + IGIK L+   +    +D   RV + L  LK
Sbjct:   14 DELSLTNCAVVSEKD--LQSGQHVTVRTTPTHKFVFTVKGHHSVLPGTIAFSLPQRKWAGLSIGQEIEVSNYNFDKSRQCIGAMTIEIDFLQKKSTDSSPYDSDKMAAEFIQHFNNQSFSVGQQLVFSFCDKLFGLLIKDIEAMDSSILKGEPASGKKQKIEVGLLVGNSQVIFEKSESSSLTLVGKAKTKEARQTIINPDWNFEKMGIGGLDKEFSDIFRRAFASRVFPPDIVEQMGCKHVKGILLFGPPGCGKTLMARQIGKMLNAREPKIVNGPEILNKYVGESEANIRKLFADAEEEQKRLGANSGLHIIIFDELDAICKQRGTGASSTGVHDTVVNQLLSKIDGVEQLNNILVIGMTNRPDLIDEALMRPGRFEVKMEIGLPDEKGRVQILNIHTAKMREFKLLASDVDVKELAAETKNYSGAELEGLVRAAQSTAMNRHIKATSTVEVDMERAEKLQVTRTDFMASLNNDIKPAFGTNQEDYSSYIMNGIIKWGDPVTRVLEDGELLVQQTKNSDRTPLVSVLLEGPPHSGKTALAAKISEDSQFPFIKICSPDKMIGHSEISKCQAIKKIFDDAYKSQLSCVVVDDIERLLDYVPIGPRFSNLVLQALLVLLKKTPPHGRKLLIIGTTSRKDVLQEMEMLDAF-STTIHVQNISSGDHLVEALELLGSFTDAERTTIAQHVK---GKRVWIGIKKLLMLIEMSLQMDQEYRVSKFLSLLK 734          
BLAST of Vesicle-fusing ATPase vs. Ensembl Zebrafish
Match: nsfb (N-ethylmaleimide-sensitive factor b [Source:ZFIN;Acc:ZDB-GENE-050808-1])

HSP 1 Score: 569.311 bits (1466), Expect = 0.000e+0
Identity = 292/666 (43.84%), Postives = 431/666 (64.71%), Query Frame = 2
Query:   35 DEFSYTNKIFFNPSDWALFKEKFITVTTASNKFIFKSDKDESVKKGCMTTNGIQRKWMYCGIGEVVECIPYSFQXXXXXXXXXXXXDLFVKSASL-KDPIDSDKLAVKYLERFHDTPVTKNQLLLFNWESINFEAKIISIQGLDVK-------SSKMNEIETGIIQSNTVFKFQRRPESKFMLSGRCKAENAAVCLINPNWDFNSIGVGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEKFDPKKGIAIRIEDLQSFKINRQHFIFAIDHDVKALYGRHDDDLQEFKENRLLKWSPQISKALSTAGLLIESLKTNDNISRKRLLIYGEPLTGTSSVAVHLAKETGFTFIRMCSMRNMIGFSEPARCLAIKKIFDDALKAPASVIILDDVERLIDYSSIGPRFSIFLVNNLIGMINCFVPKNKKMLIIGTTKHLSAMHELEFFSAFDKNCIKIPEIKTPSEII 2008
            DE S TN +  +  D+   +   I  TT + KF+F      SV  G +  +  QRKW    + + VE   Y+F         +     F++  S+  +P DSDK+A+++++ F     + +Q  +F++    F   I  I+ +D         SSK ++I+ G++  N+   F++   S   L G+ K + +   +INP+W+F  +G+GGLD+EFS IF+ AF SR+FPP+ VE++G KHV+GILLYGPPG GKTL ARQIGKMLNAREPK+VNGP IL+KYVGESEANIRKLFADAEEE K++G  S LHIIIFDEIDAICK RG+++G + V DTVVNQLL+K+DGVEQLNNIL+IGMTNR D+IDEALLRPGR+ V++E+ LP + GR+QI  IHT K+++ N+LA D+D+KELA  TKNYSGAE+E +V AA + A +        + +  E  Q+ +++R  F+ ++++D+K  +G + +D   +  N ++KWS  +S  L    LL++  K ++      +L+ G   +G +++A  +A+++ F FI++CS   MIGF+E A+C AIKKIF+DA K+  S +++DD+ERL+D+  IGPRFS  ++  L+ ++    P+ +K+LI+GTT     + E+    +F    I IP I T   ++
Sbjct:   11 DELSLTNCVVASEKDFKSGQHLTIK-TTPTQKFVFTVRTHPSVVPGTIAFSLPQRKWAGLSLNQDVEVSVYNFDPSRQYVGTMTIEIDFLQKKSIDSNPYDSDKMAIEFIQCFTAQAFSMSQQFVFSFCDKLFALVIKDIEAMDPSILRGEQNSSKKHKIDIGLLHGNSQVIFEKVESSSVNLIGKAKTKGSRQSIINPDWNFERMGIGGLDKEFSDIFRRAFASRVFPPDIVEQMGCKHVKGILLYGPPGCGKTLMARQIGKMLNAREPKVVNGPEILNKYVGESEANIRKLFADAEEEQKRLGANSGLHIIIFDEIDAICKQRGSMAGSTGVHDTVVNQLLSKIDGVEQLNNILVIGMTNRPDLIDEALLRPGRLEVKMEIGLPDETGRVQILNIHTAKMKQSNMLAKDVDVKELAVETKNYSGAELEGLVRAAQSTAMNRHIKATTQVEVDTEKAQTLQVSRSDFLASLNNDIKPAFGSNQEDYSSYIMNGIVKWSNAVSDILGDGELLVQQTKNSERTPLVTVLLEGPAHSGKTALAAKIAEQSQFPFIKICSPDKMIGFTETAKCQAIKKIFEDAYKSQLSCVVVDDIERLLDFVPIGPRFSNLVLQALLVLLKKPPPRGRKLLILGTTSRKDVLQEMGMSDSFS-TTIHIPNISTGEHLM 674          
BLAST of Vesicle-fusing ATPase vs. Ensembl Zebrafish
Match: nsfb (N-ethylmaleimide-sensitive factor b [Source:ZFIN;Acc:ZDB-GENE-050808-1])

HSP 1 Score: 567.385 bits (1461), Expect = 0.000e+0
Identity = 291/659 (44.16%), Postives = 428/659 (64.95%), Query Frame = 2
Query:   35 DEFSYTNKIFFNPSDWALFKEKFITVTTASNKFIFKSDKDESVKKGCMTTNGIQRKWMYCGIGEVVECIPYSFQXXXXXXXXXXXXDLFVKSASL-KDPIDSDKLAVKYLERFHDTPVTKNQLLLFNWESINFEAKIISIQGLDVK-------SSKMNEIETGIIQSNTVFKFQRRPESKFMLSGRCKAENAAVCLINPNWDFNSIGVGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEKFDPKKGIAIRIEDLQSFKINRQHFIFAIDHDVKALYGRHDDDLQEFKENRLLKWSPQISKALSTAGLLIESLKTNDNISRKRLLIYGEPLTGTSSVAVHLAKETGFTFIRMCSMRNMIGFSEPARCLAIKKIFDDALKAPASVIILDDVERLIDYSSIGPRFSIFLVNNLIGMINCFVPKNKKMLIIGTTKHLSAMHELEFFSAFDKNCIKIPEI 1987
            DE S TN +  +  D+   +   I  TT + KF+F      SV  G +  +  QRKW    + + VE   Y+F         +     F++  S+  +P DSDK+A+++++ F     + +Q  +F++    F   I  I+ +D         SSK ++I+ G++  N+   F++   S   L G+ K + +   +INP+W+F  +G+GGLD+EFS IF+ AF SR+FPP+ VE++G KHV+GILLYGPPG GKTL ARQIGKMLNAREPK+VNGP IL+KYVGESEANIRKLFADAEEE K++G  S LHIIIFDEIDAICK RG+++G + V DTVVNQLL+K+DGVEQLNNIL+IGMTNR D+IDEALLRPGR+ V++E+ LP + GR+QI  IHT K+++ N+LA D+D+KELA  TKNYSGAE+E +V AA + A +        + +  E  Q+ +++R  F+ ++++D+K  +G + +D   +  N ++KWS  +S  L    LL++  K ++      +L+ G   +G +++A  +A+++ F FI++CS   MIGF+E A+C AIKKIF+DA K+  S +++DD+ERL+D+  IGPRFS  ++  L+ ++    P+ +K+LI+GTT     + E+    +F    I IP I
Sbjct:   14 DELSLTNCVVASEKDFKSGQHLTIK-TTPTQKFVFTVRTHPSVVPGTIAFSLPQRKWAGLSLNQDVEVSVYNFDPSRQYVGTMTIEIDFLQKKSIDSNPYDSDKMAIEFIQCFTAQAFSMSQQFVFSFCDKLFALVIKDIEAMDPSILRGEQNSSKKHKIDIGLLHGNSQVIFEKAESSSVNLIGKAKTKGSRQSIINPDWNFERMGIGGLDKEFSDIFRRAFASRVFPPDIVEQMGCKHVKGILLYGPPGCGKTLMARQIGKMLNAREPKVVNGPEILNKYVGESEANIRKLFADAEEEQKRLGANSGLHIIIFDEIDAICKQRGSMAGSTGVHDTVVNQLLSKIDGVEQLNNILVIGMTNRPDLIDEALLRPGRLEVKMEIGLPDETGRVQILNIHTAKMKQSNMLAKDVDVKELAVETKNYSGAELEGLVRAAQSTAMNRHIKATTQVEVDTEKAQTLQVSRSDFLASLNNDIKPAFGSNQEDYSSYIMNGIVKWSNAVSDILGDGELLVQQTKNSERTPLVTVLLEGPAHSGKTALAAKIAEQSQFPFIKICSPDKMIGFTETAKCQAIKKIFEDAYKSQLSCVVVDDIERLLDFVPIGPRFSNLVLQALLVLLKKPPPRGRKLLILGTTSRKDVLQEMGMSDSFS-TTIHIPNI 670          
BLAST of Vesicle-fusing ATPase vs. Ensembl Zebrafish
Match: nsfb (N-ethylmaleimide-sensitive factor b [Source:ZFIN;Acc:ZDB-GENE-050808-1])

HSP 1 Score: 567.385 bits (1461), Expect = 0.000e+0
Identity = 291/659 (44.16%), Postives = 428/659 (64.95%), Query Frame = 2
Query:   35 DEFSYTNKIFFNPSDWALFKEKFITVTTASNKFIFKSDKDESVKKGCMTTNGIQRKWMYCGIGEVVECIPYSFQXXXXXXXXXXXXDLFVKSASL-KDPIDSDKLAVKYLERFHDTPVTKNQLLLFNWESINFEAKIISIQGLDVK-------SSKMNEIETGIIQSNTVFKFQRRPESKFMLSGRCKAENAAVCLINPNWDFNSIGVGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEKFDPKKGIAIRIEDLQSFKINRQHFIFAIDHDVKALYGRHDDDLQEFKENRLLKWSPQISKALSTAGLLIESLKTNDNISRKRLLIYGEPLTGTSSVAVHLAKETGFTFIRMCSMRNMIGFSEPARCLAIKKIFDDALKAPASVIILDDVERLIDYSSIGPRFSIFLVNNLIGMINCFVPKNKKMLIIGTTKHLSAMHELEFFSAFDKNCIKIPEI 1987
            DE S TN +  +  D+   +   I  TT + KF+F      SV  G +  +  QRKW    + + VE   Y+F         +     F++  S+  +P DSDK+A+++++ F     + +Q  +F++    F   I  I+ +D         SSK ++I+ G++  N+   F++   S   L G+ K + +   +INP+W+F  +G+GGLD+EFS IF+ AF SR+FPP+ VE++G KHV+GILLYGPPG GKTL ARQIGKMLNAREPK+VNGP IL+KYVGESEANIRKLFADAEEE K++G  S LHIIIFDEIDAICK RG+++G + V DTVVNQLL+K+DGVEQLNNIL+IGMTNR D+IDEALLRPGR+ V++E+ LP + GR+QI  IHT K+++ N+LA D+D+KELA  TKNYSGAE+E +V AA + A +        + +  E  Q+ +++R  F+ ++++D+K  +G + +D   +  N ++KWS  +S  L    LL++  K ++      +L+ G   +G +++A  +A+++ F FI++CS   MIGF+E A+C AIKKIF+DA K+  S +++DD+ERL+D+  IGPRFS  ++  L+ ++    P+ +K+LI+GTT     + E+    +F    I IP I
Sbjct:   14 DELSLTNCVVASEKDFKSGQHLTIK-TTPTQKFVFTVRTHPSVVPGTIAFSLPQRKWAGLSLNQDVEVSVYNFDPSRQYVGTMTIEIDFLQKKSIDSNPYDSDKMAIEFIQCFTAQAFSMSQQFVFSFCDKLFALVIKDIEAMDPSILRGEQNSSKKHKIDIGLLHGNSQVIFEKAESSSVNLIGKAKTKGSRQSIINPDWNFERMGIGGLDKEFSDIFRRAFASRVFPPDIVEQMGCKHVKGILLYGPPGCGKTLMARQIGKMLNAREPKVVNGPEILNKYVGESEANIRKLFADAEEEQKRLGANSGLHIIIFDEIDAICKQRGSMAGSTGVHDTVVNQLLSKIDGVEQLNNILVIGMTNRPDLIDEALLRPGRLEVKMEIGLPDETGRVQILNIHTAKMKQSNMLAKDVDVKELAVETKNYSGAELEGLVRAAQSTAMNRHIKATTQVEVDTEKAQTLQVSRSDFLASLNNDIKPAFGSNQEDYSSYIMNGIVKWSNAVSDILGDGELLVQQTKNSERTPLVTVLLEGPAHSGKTALAAKIAEQSQFPFIKICSPDKMIGFTETAKCQAIKKIFEDAYKSQLSCVVVDDIERLLDFVPIGPRFSNLVLQALLVLLKKPPPRGRKLLILGTTSRKDVLQEMGMSDSFS-TTIHIPNI 670          
BLAST of Vesicle-fusing ATPase vs. Ensembl Zebrafish
Match: nsfb (N-ethylmaleimide-sensitive factor b [Source:ZFIN;Acc:ZDB-GENE-050808-1])

HSP 1 Score: 567.385 bits (1461), Expect = 0.000e+0
Identity = 291/659 (44.16%), Postives = 428/659 (64.95%), Query Frame = 2
Query:   35 DEFSYTNKIFFNPSDWALFKEKFITVTTASNKFIFKSDKDESVKKGCMTTNGIQRKWMYCGIGEVVECIPYSFQXXXXXXXXXXXXDLFVKSASL-KDPIDSDKLAVKYLERFHDTPVTKNQLLLFNWESINFEAKIISIQGLDVK-------SSKMNEIETGIIQSNTVFKFQRRPESKFMLSGRCKAENAAVCLINPNWDFNSIGVGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEKFDPKKGIAIRIEDLQSFKINRQHFIFAIDHDVKALYGRHDDDLQEFKENRLLKWSPQISKALSTAGLLIESLKTNDNISRKRLLIYGEPLTGTSSVAVHLAKETGFTFIRMCSMRNMIGFSEPARCLAIKKIFDDALKAPASVIILDDVERLIDYSSIGPRFSIFLVNNLIGMINCFVPKNKKMLIIGTTKHLSAMHELEFFSAFDKNCIKIPEI 1987
            DE S TN +  +  D+   +   I  TT + KF+F      SV  G +  +  QRKW    + + VE   Y+F         +     F++  S+  +P DSDK+A+++++ F     + +Q  +F++    F   I  I+ +D         SSK ++I+ G++  N+   F++   S   L G+ K + +   +INP+W+F  +G+GGLD+EFS IF+ AF SR+FPP+ VE++G KHV+GILLYGPPG GKTL ARQIGKMLNAREPK+VNGP IL+KYVGESEANIRKLFADAEEE K++G  S LHIIIFDEIDAICK RG+++G + V DTVVNQLL+K+DGVEQLNNIL+IGMTNR D+IDEALLRPGR+ V++E+ LP + GR+QI  IHT K+++ N+LA D+D+KELA  TKNYSGAE+E +V AA + A +        + +  E  Q+ +++R  F+ ++++D+K  +G + +D   +  N ++KWS  +S  L    LL++  K ++      +L+ G   +G +++A  +A+++ F FI++CS   MIGF+E A+C AIKKIF+DA K+  S +++DD+ERL+D+  IGPRFS  ++  L+ ++    P+ +K+LI+GTT     + E+    +F    I IP I
Sbjct:   14 DELSLTNCVVASEKDFKSGQHLTIK-TTPTQKFVFTVRTHPSVVPGTIAFSLPQRKWAGLSLNQDVEVSVYNFDPSRQYVGTMTIEIDFLQKKSIDSNPYDSDKMAIEFIQCFTAQAFSMSQQFVFSFCDKLFALVIKDIEAMDPSILRGEQNSSKKHKIDIGLLHGNSQVIFEKAESSSVNLIGKAKTKGSRQSIINPDWNFERMGIGGLDKEFSDIFRRAFASRVFPPDIVEQMGCKHVKGILLYGPPGCGKTLMARQIGKMLNAREPKVVNGPEILNKYVGESEANIRKLFADAEEEQKRLGANSGLHIIIFDEIDAICKQRGSMAGSTGVHDTVVNQLLSKIDGVEQLNNILVIGMTNRPDLIDEALLRPGRLEVKMEIGLPDETGRVQILNIHTAKMKQSNMLAKDVDVKELAVETKNYSGAELEGLVRAAQSTAMNRHIKATTQVEVDTEKAQTLQVSRSDFLASLNNDIKPAFGSNQEDYSSYIMNGIVKWSNAVSDILGDGELLVQQTKNSERTPLVTVLLEGPAHSGKTALAAKIAEQSQFPFIKICSPDKMIGFTETAKCQAIKKIFEDAYKSQLSCVVVDDIERLLDFVPIGPRFSNLVLQALLVLLKKPPPRGRKLLILGTTSRKDVLQEMGMSDSFS-TTIHIPNI 670          
BLAST of Vesicle-fusing ATPase vs. Ensembl Xenopus
Match: fosl2 (FOS-like antigen 2 [Source:Xenbase;Acc:XB-GENE-6258093])

HSP 1 Score: 580.096 bits (1494), Expect = 0.000e+0
Identity = 303/732 (41.39%), Postives = 458/732 (62.57%), Query Frame = 2
Query:   35 DEFSYTNKIFFNPSDWALFKEKFITVTTASN-KFIFKSDKDESVKKGCMTTNGIQRKWMYCGIGEVVECIPYSFQXXXXXXXXXXXXDLFVKSASL-KDPIDSDKLAVKYLERFHDTPVTKNQLLLFNWESINFEAKIISIQGLD-------VKSSKMNEIETGIIQSNTVFKFQRRPESKFMLSGRCKAENAAVCLINPNWDFNSIGVGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEKFDPKKGIAIRIEDLQSFKINRQHFIFAIDHDVKALYGRHDDDLQEFKENRLLKWSPQISKALSTAGLLIESLKTNDNISRKRLLIYGEPLTGTSSVAVHLAKETGFTFIRMCSMRNMIGFSEPARCLAIKKIFDDALKAPASVIILDDVERLIDYSSIGPRFSIFLVNNLIGMINCFVPKNKKMLIIGTTKHLSAMHELEFFSAFDKNCIKIPEIKTPSEIISVXXXXXXXXXXXXXXXXNHLQMGDCSDLAIGIKNLMENCKDLKSVDPARRVDELLMKLKSSSEF 2203
            DE S TN    N  D+     + + V T+ N ++IF      ++  G +  +  QRKW    IG+ VE   Y+F         +     F++  S+  +P D+D++A +++++F++   +  Q L+F++    F   +  I+ +D         + K  +IE G++  N+   F++   S   L+G+ K       +INP+W+F  +G+GGLD+EFS IF+ AF SR+FPPE VE++G KHV+GILL+GPPG GKTL ARQIGKMLNAREPK+VNGP IL+KYVGESEANIRKLFADAEEE +++G  S LHIIIFDEIDAICK RG+++G + V DTVVNQLL+K+DGVEQLNNIL+IGMTNR D+IDEALLRPGR+ V++E+ LP + GRLQI  IHT ++RE ++L+ D+D+ ELA  TKN+SGAE+E +V AA + A +        + + +E  +  ++ +  F  ++ +D+K  +G + +D   +  N ++KW   +++ L    LL++  K +D      +L+ G P +G +++A  +++E+ F FI++CS   MIGFSE A+C AIKKIF+DA K+  S +++DD+ERL+DY  IGPRFS  ++  L+ ++    P+ +K+LIIGTT     + E+E   AF    I++P I T  +++  L     + D++   I   ++      + IGIK L+   +    +DP  RV + L  LK  ++ 
Sbjct:   14 DELSITNCAVVNEKDFQ--SGQHVNVRTSPNHRYIFTVKTHHTILPGSIAFSLPQRKWAGLSIGQDVEVGVYTFDKTKQCIGTMTIEIDFLQKKSIDSNPYDTDRMAAEFIQQFNNQAFSVGQQLVFSFNDKLFGLVVKDIEAMDPSILKGEQSTGKRQKIEVGLVVGNSQVVFEKADSSSLNLTGKSKTRQDRQSIINPDWNFEKMGIGGLDKEFSDIFRRAFASRVFPPEIVEQMGCKHVKGILLFGPPGCGKTLMARQIGKMLNAREPKVVNGPEILNKYVGESEANIRKLFADAEEEQRRLGANSGLHIIIFDEIDAICKQRGSMAGSTGVHDTVVNQLLSKIDGVEQLNNILVIGMTNRPDLIDEALLRPGRLEVKMEIGLPDEKGRLQILHIHTARMREHSLLSSDVDVGELAQETKNFSGAELEGLVRAAQSTAMNRHIKATNKVEVDMEKAERLQVTKGDFFTSLGNDIKPAFGTNQEDYASYIMNGIIKWGDPVTRVLDDGELLVQQTKNSDRTPLVSVLLEGPPHSGKTALAAKISEESNFPFIKICSPEKMIGFSETAKCQAIKKIFEDAYKSQLSCVVVDDIERLLDYVPIGPRFSNLVLQALLVLLKKAPPQGRKLLIIGTTSRKDVLQEMEMLDAFS-TTIRVPNIVTGEQLMEALELLGGFKDNERSVIAQQVK---GKKVYIGIKKLLMLIEMSMQMDPEYRVSKFLALLKEEAKL 739          
BLAST of Vesicle-fusing ATPase vs. Ensembl Xenopus
Match: fosl2 (FOS-like antigen 2 [Source:Xenbase;Acc:XB-GENE-6258093])

HSP 1 Score: 579.326 bits (1492), Expect = 0.000e+0
Identity = 303/730 (41.51%), Postives = 457/730 (62.60%), Query Frame = 2
Query:   35 DEFSYTNKIFFNPSDWALFKEKFITVTTASN-KFIFKSDKDESVKKGCMTTNGIQRKWMYCGIGEVVECIPYSFQXXXXXXXXXXXXDLFVKSASL-KDPIDSDKLAVKYLERFHDTPVTKNQLLLFNWESINFEAKIISIQGLDVK-------SSKMNEIETGIIQSNTVFKFQRRPESKFMLSGRCKAENAAVCLINPNWDFNSIGVGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEKFDPKKGIAIRIEDLQSFKINRQHFIFAIDHDVKALYGRHDDDLQEFKENRLLKWSPQISKALSTAGLLIESLKTNDNISRKRLLIYGEPLTGTSSVAVHLAKETGFTFIRMCSMRNMIGFSEPARCLAIKKIFDDALKAPASVIILDDVERLIDYSSIGPRFSIFLVNNLIGMINCFVPKNKKMLIIGTTKHLSAMHELEFFSAFDKNCIKIPEIKTPSEIISVXXXXXXXXXXXXXXXXNHLQMGDCSDLAIGIKNLMENCKDLKSVDPARRVDELLMKLKSSS 2197
            DE S TN    N  D+     + + V T+ N ++IF      ++  G +  +  QRKW    IG+ VE   Y+F         +     F++  S+  +P D+D++A +++++F++   +  Q L+F++    F   +  I+ +D         + K  +IE G++  N+   F++   S   L+G+ K       +INP+W+F  +G+GGLD+EFS IF+ AF SR+FPPE VE++G KHV+GILL+GPPG GKTL ARQIGKMLNAREPK+VNGP IL+KYVGESEANIRKLFADAEEE +++G  S LHIIIFDEIDAICK RG+++G + V DTVVNQLL+K+DGVEQLNNIL+IGMTNR D+IDEALLRPGR+ V++E+ LP + GRLQI  IHT ++RE ++L+ D+D+ ELA  TKN+SGAE+E +V AA + A +        + + +E  +  ++ +  F  ++ +D+K  +G + +D   +  N ++KW   +++ L    LL++  K +D      +L+ G P +G +++A  +++E+ F FI++CS   MIGFSE A+C AIKKIF+DA K+  S +++DD+ERL+DY  IGPRFS  ++  L+ ++    P+ +K+LIIGTT     + E+E   AF    I++P I T  +++  L     + D++   I   ++      + IGIK L+   +    +DP  RV + L  LK  +
Sbjct:   15 DELSITNCAVVNEKDFQ--SGQHVNVRTSPNHRYIFTVKTHHTILPGSIAFSLPQRKWAGLSIGQDVEVGVYTFDKTKQCIGTMTIEIDFLQKKSIDSNPYDTDRMAAEFIQQFNNQAFSVGQQLVFSFNDKLFGLVVKDIEAMDPSILKGEQSTGKRQKIEVGLVVGNSQVVFEKADSSSLNLTGKSKTRQDRQSIINPDWNFEKMGIGGLDKEFSDIFRRAFASRVFPPEIVEQMGCKHVKGILLFGPPGCGKTLMARQIGKMLNAREPKVVNGPEILNKYVGESEANIRKLFADAEEEQRRLGANSGLHIIIFDEIDAICKQRGSMAGSTGVHDTVVNQLLSKIDGVEQLNNILVIGMTNRPDLIDEALLRPGRLEVKMEIGLPDEKGRLQILHIHTARMREHSLLSSDVDVGELAQETKNFSGAELEGLVRAAQSTAMNRHIKATNKVEVDMEKAERLQVTKGDFFTSLGNDIKPAFGTNQEDYASYIMNGIIKWGDPVTRVLDDGELLVQQTKNSDRTPLVSVLLEGPPHSGKTALAAKISEESNFPFIKICSPEKMIGFSETAKCQAIKKIFEDAYKSQLSCVVVDDIERLLDYVPIGPRFSNLVLQALLVLLKKAPPQGRKLLIIGTTSRKDVLQEMEMLDAFS-TTIRVPNIVTGEQLMEALELLGGFKDNERSVIAQQVK---GKKVYIGIKKLLMLIEMSMQMDPEYRVSKFLALLKEEA 738          
BLAST of Vesicle-fusing ATPase vs. Ensembl Xenopus
Match: fosl2 (FOS-like antigen 2 [Source:Xenbase;Acc:XB-GENE-6258093])

HSP 1 Score: 579.326 bits (1492), Expect = 0.000e+0
Identity = 303/733 (41.34%), Postives = 459/733 (62.62%), Query Frame = 2
Query:   35 DEFSYTNKIFFNPSDWALFKEKFITVTTASN-KFIFKSDKDESVKKGCMTTNGIQRKWMYCGIGEVVECIPYSFQXXXXXXXXXXXXDLFVKSASL-KDPIDSDKLAVKYLERFHDTPVTKNQLLLFNWESINFEAKIISIQGLDVK-------SSKMNEIETGIIQSNTVFKFQRRPESKFMLSGRCKAENAAVCLINPNWDFNSIGVGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEKFDPKKGIAIRIEDLQSFKINRQHFIFAIDHDVKALYGRHDDDLQEFKENRLLKWSPQISKALSTAGLLIESLKTNDNISRKRLLIYGEPLTGTSSVAVHLAKETGFTFIRMCSMRNMIGFSEPARCLAIKKIFDDALKAPASVIILDDVERLIDYSSIGPRFSIFLVNNLIGMINCFVPKNKKMLIIGTTKHLSAMHELEFFSAFDKNCIKIPEIKTPSEIISVXXXXXXXXXXXXXXXXNHLQMGDCSDLAIGIKNLMENCKDLKSVDPARRVDELLMKLKSSSEFQ 2206
            DE S TN    N  D+     + + V T+ N ++IF      ++  G +  +  QRKW    IG+ VE   Y+F         +     F++  S+  +P D+D++A +++++F++   +  Q L+F++    F   +  I+ +D         + K  +IE G++  N+   F++   S   L+G+ K       +INP+W+F  +G+GGLD+EFS IF+ AF SR+FPPE VE++G KHV+GILL+GPPG GKTL ARQIGKMLNAREPK+VNGP IL+KYVGESEANIRKLFADAEEE +++G  S LHIIIFDEIDAICK RG+++G + V DTVVNQLL+K+DGVEQLNNIL+IGMTNR D+IDEALLRPGR+ V++E+ LP + GRLQI  IHT ++RE ++L+ D+D+ ELA  TKN+SGAE+E +V AA + A +        + + +E  +  ++ +  F  ++ +D+K  +G + +D   +  N ++KW   +++ L    LL++  K +D      +L+ G P +G +++A  +++E+ F FI++CS   MIGFSE A+C AIKKIF+DA K+  S +++DD+ERL+DY  IGPRFS  ++  L+ ++    P+ +K+LIIGTT     + E+E   AF    I++P I T  +++  L     + D++   I   ++      + IGIK L+   +    +DP  RV + L  LK  + ++
Sbjct:   14 DELSITNCAVVNEKDFQ--SGQHVNVRTSPNHRYIFTVKTHHTILPGSIAFSLPQRKWAGLSIGQDVEVGVYTFDKTKQCIGTMTIEIDFLQKKSIDSNPYDTDRMAAEFIQQFNNQAFSVGQQLVFSFNDKLFGLVVKDIEAMDPSILKGEQSTGKRQKIEVGLVVGNSQVVFEKADSSSLNLTGKSKTRQDRQSIINPDWNFEKMGIGGLDKEFSDIFRRAFASRVFPPEIVEQMGCKHVKGILLFGPPGCGKTLMARQIGKMLNAREPKVVNGPEILNKYVGESEANIRKLFADAEEEQRRLGANSGLHIIIFDEIDAICKQRGSMAGSTGVHDTVVNQLLSKIDGVEQLNNILVIGMTNRPDLIDEALLRPGRLEVKMEIGLPDEKGRLQILHIHTARMREHSLLSSDVDVGELAQETKNFSGAELEGLVRAAQSTAMNRHIKATNKVEVDMEKAERLQVTKGDFFTSLGNDIKPAFGTNQEDYASYIMNGIIKWGDPVTRVLDDGELLVQQTKNSDRTPLVSVLLEGPPHSGKTALAAKISEESNFPFIKICSPEKMIGFSETAKCQAIKKIFEDAYKSQLSCVVVDDIERLLDYVPIGPRFSNLVLQALLVLLKKAPPQGRKLLIIGTTSRKDVLQEMEMLDAFS-TTIRVPNIVTGEQLMEALELLGGFKDNERSVIAQQVK---GKKVYIGIKKLLMLIEMSMQMDPEYRVSKFLALLKEEAAWK 740          
BLAST of Vesicle-fusing ATPase vs. Ensembl Xenopus
Match: fosl2 (FOS-like antigen 2 [Source:Xenbase;Acc:XB-GENE-6258093])

HSP 1 Score: 578.941 bits (1491), Expect = 0.000e+0
Identity = 303/730 (41.51%), Postives = 457/730 (62.60%), Query Frame = 2
Query:   35 DEFSYTNKIFFNPSDWALFKEKFITVTTASN-KFIFKSDKDESVKKGCMTTNGIQRKWMYCGIGEVVECIPYSFQXXXXXXXXXXXXDLFVKSASL-KDPIDSDKLAVKYLERFHDTPVTKNQLLLFNWESINFEAKIISIQGLDVK-------SSKMNEIETGIIQSNTVFKFQRRPESKFMLSGRCKAENAAVCLINPNWDFNSIGVGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEKFDPKKGIAIRIEDLQSFKINRQHFIFAIDHDVKALYGRHDDDLQEFKENRLLKWSPQISKALSTAGLLIESLKTNDNISRKRLLIYGEPLTGTSSVAVHLAKETGFTFIRMCSMRNMIGFSEPARCLAIKKIFDDALKAPASVIILDDVERLIDYSSIGPRFSIFLVNNLIGMINCFVPKNKKMLIIGTTKHLSAMHELEFFSAFDKNCIKIPEIKTPSEIISVXXXXXXXXXXXXXXXXNHLQMGDCSDLAIGIKNLMENCKDLKSVDPARRVDELLMKLKSSS 2197
            DE S TN    N  D+     + + V T+ N ++IF      ++  G +  +  QRKW    IG+ VE   Y+F         +     F++  S+  +P D+D++A +++++F++   +  Q L+F++    F   +  I+ +D         + K  +IE G++  N+   F++   S   L+G+ K       +INP+W+F  +G+GGLD+EFS IF+ AF SR+FPPE VE++G KHV+GILL+GPPG GKTL ARQIGKMLNAREPK+VNGP IL+KYVGESEANIRKLFADAEEE +++G  S LHIIIFDEIDAICK RG+++G + V DTVVNQLL+K+DGVEQLNNIL+IGMTNR D+IDEALLRPGR+ V++E+ LP + GRLQI  IHT ++RE ++L+ D+D+ ELA  TKN+SGAE+E +V AA + A +        + + +E  +  ++ +  F  ++ +D+K  +G + +D   +  N ++KW   +++ L    LL++  K +D      +L+ G P +G +++A  +++E+ F FI++CS   MIGFSE A+C AIKKIF+DA K+  S +++DD+ERL+DY  IGPRFS  ++  L+ ++    P+ +K+LIIGTT     + E+E   AF    I++P I T  +++  L     + D++   I   ++      + IGIK L+   +    +DP  RV + L  LK  +
Sbjct:   13 DELSITNCAVVNEKDFQ--SGQHVNVRTSPNHRYIFTVKTHHTILPGSIAFSLPQRKWAGLSIGQDVEVGVYTFDKTKQCIGTMTIEIDFLQKKSIDSNPYDTDRMAAEFIQQFNNQAFSVGQQLVFSFNDKLFGLVVKDIEAMDPSILKGEQSTGKRQKIEVGLVVGNSQVVFEKADSSSLNLTGKSKTRQDRQSIINPDWNFEKMGIGGLDKEFSDIFRRAFASRVFPPEIVEQMGCKHVKGILLFGPPGCGKTLMARQIGKMLNAREPKVVNGPEILNKYVGESEANIRKLFADAEEEQRRLGANSGLHIIIFDEIDAICKQRGSMAGSTGVHDTVVNQLLSKIDGVEQLNNILVIGMTNRPDLIDEALLRPGRLEVKMEIGLPDEKGRLQILHIHTARMREHSLLSSDVDVGELAQETKNFSGAELEGLVRAAQSTAMNRHIKATNKVEVDMEKAERLQVTKGDFFTSLGNDIKPAFGTNQEDYASYIMNGIIKWGDPVTRVLDDGELLVQQTKNSDRTPLVSVLLEGPPHSGKTALAAKISEESNFPFIKICSPEKMIGFSETAKCQAIKKIFEDAYKSQLSCVVVDDIERLLDYVPIGPRFSNLVLQALLVLLKKAPPQGRKLLIIGTTSRKDVLQEMEMLDAFS-TTIRVPNIVTGEQLMEALELLGGFKDNERSVIAQQVK---GKKVYIGIKKLLMLIEMSMQMDPEYRVSKFLALLKEEA 736          
BLAST of Vesicle-fusing ATPase vs. Ensembl Xenopus
Match: fosl2 (FOS-like antigen 2 [Source:Xenbase;Acc:XB-GENE-6258093])

HSP 1 Score: 578.556 bits (1490), Expect = 0.000e+0
Identity = 303/730 (41.51%), Postives = 457/730 (62.60%), Query Frame = 2
Query:   35 DEFSYTNKIFFNPSDWALFKEKFITVTTASN-KFIFKSDKDESVKKGCMTTNGIQRKWMYCGIGEVVECIPYSFQXXXXXXXXXXXXDLFVKSASL-KDPIDSDKLAVKYLERFHDTPVTKNQLLLFNWESINFEAKIISIQGLDVK-------SSKMNEIETGIIQSNTVFKFQRRPESKFMLSGRCKAENAAVCLINPNWDFNSIGVGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEKFDPKKGIAIRIEDLQSFKINRQHFIFAIDHDVKALYGRHDDDLQEFKENRLLKWSPQISKALSTAGLLIESLKTNDNISRKRLLIYGEPLTGTSSVAVHLAKETGFTFIRMCSMRNMIGFSEPARCLAIKKIFDDALKAPASVIILDDVERLIDYSSIGPRFSIFLVNNLIGMINCFVPKNKKMLIIGTTKHLSAMHELEFFSAFDKNCIKIPEIKTPSEIISVXXXXXXXXXXXXXXXXNHLQMGDCSDLAIGIKNLMENCKDLKSVDPARRVDELLMKLKSSS 2197
            DE S TN    N  D+     + + V T+ N ++IF      ++  G +  +  QRKW    IG+ VE   Y+F         +     F++  S+  +P D+D++A +++++F++   +  Q L+F++    F   +  I+ +D         + K  +IE G++  N+   F++   S   L+G+ K       +INP+W+F  +G+GGLD+EFS IF+ AF SR+FPPE VE++G KHV+GILL+GPPG GKTL ARQIGKMLNAREPK+VNGP IL+KYVGESEANIRKLFADAEEE +++G  S LHIIIFDEIDAICK RG+++G + V DTVVNQLL+K+DGVEQLNNIL+IGMTNR D+IDEALLRPGR+ V++E+ LP + GRLQI  IHT ++RE ++L+ D+D+ ELA  TKN+SGAE+E +V AA + A +        + + +E  +  ++ +  F  ++ +D+K  +G + +D   +  N ++KW   +++ L    LL++  K +D      +L+ G P +G +++A  +++E+ F FI++CS   MIGFSE A+C AIKKIF+DA K+  S +++DD+ERL+DY  IGPRFS  ++  L+ ++    P+ +K+LIIGTT     + E+E   AF    I++P I T  +++  L     + D++   I   ++      + IGIK L+   +    +DP  RV + L  LK  +
Sbjct:   18 DELSITNCAVVNEKDFQ--SGQHVNVRTSPNHRYIFTVKTHHTILPGSIAFSLPQRKWAGLSIGQDVEVGVYTFDKTKQCIGTMTIEIDFLQKKSIDSNPYDTDRMAAEFIQQFNNQAFSVGQQLVFSFNDKLFGLVVKDIEAMDPSILKGEQSTGKRQKIEVGLVVGNSQVVFEKADSSSLNLTGKSKTRQDRQSIINPDWNFEKMGIGGLDKEFSDIFRRAFASRVFPPEIVEQMGCKHVKGILLFGPPGCGKTLMARQIGKMLNAREPKVVNGPEILNKYVGESEANIRKLFADAEEEQRRLGANSGLHIIIFDEIDAICKQRGSMAGSTGVHDTVVNQLLSKIDGVEQLNNILVIGMTNRPDLIDEALLRPGRLEVKMEIGLPDEKGRLQILHIHTARMREHSLLSSDVDVGELAQETKNFSGAELEGLVRAAQSTAMNRHIKATNKVEVDMEKAERLQVTKGDFFTSLGNDIKPAFGTNQEDYASYIMNGIIKWGDPVTRVLDDGELLVQQTKNSDRTPLVSVLLEGPPHSGKTALAAKISEESNFPFIKICSPEKMIGFSETAKCQAIKKIFEDAYKSQLSCVVVDDIERLLDYVPIGPRFSNLVLQALLVLLKKAPPQGRKLLIIGTTSRKDVLQEMEMLDAFS-TTIRVPNIVTGEQLMEALELLGGFKDNERSVIAQQVK---GKKVYIGIKKLLMLIEMSMQMDPEYRVSKFLALLKEEA 741          
BLAST of Vesicle-fusing ATPase vs. Ensembl Mouse
Match: Nsf (N-ethylmaleimide sensitive fusion protein [Source:MGI Symbol;Acc:MGI:104560])

HSP 1 Score: 586.645 bits (1511), Expect = 0.000e+0
Identity = 294/661 (44.48%), Postives = 431/661 (65.20%), Query Frame = 2
Query:   35 DEFSYTNKIFFNPSDWALFKEKFITVTTASNKFIFKSDKDESVKKGCMTTNGIQRKWMYCGIGEVVECIPYSFQXXXXXXXXXXXXDLFVKSASL-KDPIDSDKLAVKYLERFHDTPVTKNQLLLFNWESINFEAKIISIQGLDVK-------SSKMNEIETGIIQSNTVFKFQRRPESKFMLSGRCKAENAAVCLINPNWDFNSIGVGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEKFDPKKGIAIRIEDLQSFKINRQHFIFAIDHDVKALYGRHDDDLQEFKENRLLKWSPQISKALSTAGLLIESLKTNDNISRKRLLIYGEPLTGTSSVAVHLAKETGFTFIRMCSMRNMIGFSEPARCLAIKKIFDDALKAPASVIILDDVERLIDYSSIGPRFSIFLVNNLIGMINCFVPKNKKMLIIGTTKHLSAMHELEFFSAFDKNCIKIPEIKT 1993
            DE S +N    N  D+    +  +  T+ ++K+IF      SV  GC+  +  QRKW    IG+ +E   YSF         +     F++  ++  +P D+DK+A +++++F++   +  Q L+F++    F   +  I+ +D         S K  +IE G++  N+   F++   S   L G+ K +     +INP+W+F  +G+GGLD+EFS IF+ AF SR+FPPE VE++G KHV+GILLYGPPG GKTL ARQIGKMLNAREPK+VNGP IL+KYVGESEANIRKLFADAEEE +++G  S LHIIIFDEIDAICK RG+++G + V DTVVNQLL+K+DGVEQLNNIL+IGMTNR D+IDEALLRPGR+ V++E+ LP + GRLQI  IHT ++R   +L+ D+D+KELA  TKN+SGAE+E +V AA + A +        + + +E  +S ++ R  F+ ++++D+K  +G + +D   +  N ++KW   +++ L    LL++  K +D      +L+ G P +G +++A  +A+E+ F FI++CS   MIGFSE A+C A+KKIFDDA K+  S +++DD+ERL+DY  IGPRFS  ++  L+ ++    P+ +K+LIIGTT     + E+E  +AF    I +P I T
Sbjct:   14 DELSLSNCAVVNEKDFQ-SGQHVMVRTSPNHKYIFTLRTHPSVVPGCIAFSLPQRKWAGLSIGQDIEVALYSFDKAKQCIGTMTIEIDFLQKKNIDSNPYDTDKMAAEFIQQFNNQAFSVGQQLVFSFNDKLFGLLVKDIEAMDPSILKGEPASGKRQKIEVGLVVGNSQVAFEKAENSSLNLIGKAKTKENRQSIINPDWNFEKMGIGGLDKEFSDIFRRAFASRVFPPEIVEQMGCKHVKGILLYGPPGCGKTLLARQIGKMLNAREPKVVNGPEILNKYVGESEANIRKLFADAEEEQRRLGANSGLHIIIFDEIDAICKQRGSMAGSTGVHDTVVNQLLSKIDGVEQLNNILVIGMTNRPDLIDEALLRPGRLEVKMEIGLPDEKGRLQILHIHTARMRGHQLLSADVDIKELAVETKNFSGAELEGLVRAAQSTAMNRHIKASTKVEVDMEKAESLQVTRGDFLASLENDIKPAFGTNQEDYASYIMNGIIKWGDPVTRVLDDGELLVQQTKNSDRTPLVSVLLEGPPHSGKTALAAKIAEESNFPFIKICSPDKMIGFSETAKCQAMKKIFDDAYKSQLSCVVVDDIERLLDYVPIGPRFSNLVLQALLVLLKKAPPQGRKLLIIGTTSRKDVLQEMEMLNAFS-TTIHVPNIAT 672          
BLAST of Vesicle-fusing ATPase vs. Ensembl Mouse
Match: Vcp (valosin containing protein [Source:MGI Symbol;Acc:MGI:99919])

HSP 1 Score: 164.081 bits (414), Expect = 2.135e-41
Identity = 129/417 (30.94%), Postives = 209/417 (50.12%), Query Frame = 2
Query:  617 FNSIG---VGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAA----------LANAQDEKFDPK--KGIAIRIEDL-----QSFKINRQHFIFAIDHDVKALYGRHDDDLQEFKENRLLKWSPQISKALSTAGLLIESLKTNDNISRKRLLIYGEPLTGTSSVAVHLAKETGFTFIRMCS---MRNMIGFSEPARCLAIKKIFDDALKAPASVIILDDVERL 1798
             N +G   +GG  ++ +QI +   L  L  P   + +G+K  RGILLYGPPGTGKTL AR +     A    ++NGP I+ K  GESE+N+RK F +AE        ++A  II  DE+DAI   R    G   V+  +V+QLL  MDG++Q  +++++  TNR + ID AL R GR   ++++ +P   GRL+I +IHTK ++    LADD+DL+++A  T  + GA++ A+   A          L + +DE  D +    +A+ ++D      QS     +  +  +        G  +D  +E +E  L+++  +        G+             K +L YG P  G + +A  +A E    FI +     +    G SE      +++IFD A +A   V+  D+++ +
Sbjct:  198 LNEVGYDDIGGCRKQLAQIKEMVELP-LRHPALFKAIGVKPPRGILLYGPPGTGKTLIARAVANETGAFF-FLINGPEIMSKLAGESESNLRKAFEEAE--------KNAPAIIFIDELDAIAPKREKTHG--EVERRIVSQLLTLMDGLKQRAHVIVMAATNRPNSIDPALRRFGRFDREVDIGIPDATGRLEILQIHTKNMK----LADDVDLEQVANETHGHVGADLAALCSEAALQAIRKKMDLIDLEDETIDAEVMNSLAVTMDDFRWALSQSNPSALRETVVEVPQVTWEDIGGLEDVKRELQE--LVQYPVEHPDKFLKFGM----------TPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESE----ANVREIFDKARQAAPCVLFFDELDSI 582          

HSP 2 Score: 143.28 bits (360), Expect = 9.357e-35
Identity = 87/230 (37.83%), Postives = 133/230 (57.83%), Query Frame = 2
Query:  632 VGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSA-VQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAA 1318
            +GGL++   ++ +        P +F++  G+   +G+L YGPPG GKTL A+ I     A    I  GP +L  + GESEAN+R++F  A         Q+A  ++ FDE+D+I KARG   G      D V+NQ+L +MDG+    N+ IIG TNR D+ID A+LRPGR+   I +PLP +  R+ I + + +    K+ +A D+DL+ LA +T  +SGA++  +   A
Sbjct:  479 IGGLEDVKRELQELVQYPVEHPDKFLK-FGMTPSKGVLFYGPPGCGKTLLAKAIANECQANFISI-KGPELLTMWFGESEANVREIFDKA--------RQAAPCVLFFDELDSIAKARGGNIGDGGGAADRVINQILTEMDGMSTKKNVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDEKSRVAILKANLR----KSPVAKDVDLEFLAKMTNGFSGADLTEICQRA 694          
BLAST of Vesicle-fusing ATPase vs. Ensembl Mouse
Match: Psmc5 (protease (prosome, macropain) 26S subunit, ATPase 5 [Source:MGI Symbol;Acc:MGI:105047])

HSP 1 Score: 149.828 bits (377), Expect = 1.001e-38
Identity = 94/239 (39.33%), Postives = 137/239 (57.32%), Query Frame = 2
Query:  632 VGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKAR--GNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEK 1342
            +GGLD++  +I K      +  PE  E LGI   +G+LLYGPPGTGKTL AR +    +    + V+G  ++ K++GE    +R+LF         M  + A  II  DEID+I  +R  G   G S VQ T++ +LL ++DG E   NI +I  TNR D++D ALLRPGR+  +IE P P++  RL I +IH++K+     L   I+L+++A L    SGAE++ V   A   A  E+
Sbjct:  151 IGGLDKQIKEI-KEVIELPVKHPELFEALGIAQPKGVLLYGPPGTGKTLLARAVAHHTDCTFIR-VSGSELVQKFIGEGARMVRELFV--------MAREHAPSIIFMDEIDSIGSSRLEGGSGGDSEVQRTML-ELLNQLDGFEATKNIKVIMATNRIDILDSALLRPGRIDRKIEFPPPNEEARLDILKIHSRKMN----LTRGINLRKIAELMPGASGAEVKGVCTEAGMYALRER 374          
BLAST of Vesicle-fusing ATPase vs. Ensembl Mouse
Match: Psmc1 (protease (prosome, macropain) 26S subunit, ATPase 1 [Source:MGI Symbol;Acc:MGI:106054])

HSP 1 Score: 150.599 bits (379), Expect = 1.362e-38
Identity = 90/231 (38.96%), Postives = 138/231 (59.74%), Query Frame = 2
Query:  632 VGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKAR--GNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAA 1318
            +GGLD +  +I +S  L  L  PE+ EE+GIK  +G++LYGPPGTGKTL A+ +    +A   ++V G  ++ KY+G+    +R+LF  AEE         A  I+  DEIDAI   R   N  G   +Q T++ +LL ++DG +   ++ +I  TNR + +D AL+RPGR+  +IE PLP +  + +IF+IHT ++     LADD+ L +L     + SGA+I+A+   A
Sbjct:  187 IGGLDNQIQEIKESVELP-LTHPEYYEEMGIKPPKGVILYGPPGTGKTLLAKAVANQTSATFLRVV-GSELIQKYLGDGPKLVRELFRVAEEH--------APSIVFIDEIDAIGTKRYDSNSGGEREIQRTML-ELLNQLDGFDSRGDVKVIMATNRIETLDPALIRPGRIDRKIEFPLPDEKTKKRIFQIHTSRM----TLADDVTLDDLIMAKDDLSGADIKAICTEA 402          
BLAST of Vesicle-fusing ATPase vs. Ensembl Mouse
Match: Spata5 (spermatogenesis associated 5 [Source:MGI Symbol;Acc:MGI:1927170])

HSP 1 Score: 153.295 bits (386), Expect = 7.422e-38
Identity = 98/249 (39.36%), Postives = 137/249 (55.02%), Query Frame = 2
Query:  605 PNWDFNSIGVGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKI----VNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDE 1339
            PN  ++ IG  GL E      K A    L  P+    +GI+  +G+LLYGPPG  KT+ A+ +     A E  +    + GP +++KYVGESE  +R++F  A           A  II FDE+DA+   RG+ SG   V D V+ QLL +MDG+EQL N+ ++  TNR D ID+AL+RPGR+   I VPLP    R +I  +    +     +++++DL EL   T  YSGAEI AV   A   A +E
Sbjct:  622 PNVSWSDIG--GL-ENIKLKLKQAVEWPLKHPKSFNRMGIQPPKGVLLYGPPGCSKTMIAKAL-----ANESGLNFLAIKGPELMNKYVGESERAVREIFRKAR--------AVAPSIIFFDELDALAVERGSSSGAGNVADRVLAQLLTEMDGIEQLKNVTVLAATNRPDRIDKALMRPGRIDRIIYVPLPDAATRREILNLQFHSMP----ISNEVDLDELVLQTDTYSGAEIIAVCKEAALLALEE 850          

HSP 2 Score: 145.976 bits (367), Expect = 1.577e-35
Identity = 124/409 (30.32%), Postives = 191/409 (46.70%), Query Frame = 2
Query:  632 VGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNN---ILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAV-----------VMAALANAQDEKFDPKKGIAIRIED-LQSFKINRQHFIFAIDHDVKALYGRHDDDLQ--EFKENRLLKWSPQISKALSTAGLLIESLKTNDNISRKRLLIYGEPLTGTSSVAVHLAKETGFTFIRMCS---MRNMIGFSEPARCLAIKKIFDDALKAPASVIILDDVERL 1798
            +GGL+ +   I +   L  L  PE  +  GI   RG+LLYGPPGTGKT+ AR +   + A    ++NGP I+ K+ GE+EA +R++FA+A              II  DE+DA+C  R      S V+  VV  LL  MDG+    +   +L++G TNR   +D AL RPGR   +IE+ +P+   RL I +   K LR    L    +L  LA     Y GA+++A+           V+    N  D K      + I + D LQ     R   +  +  DV  +       L+  + K  + ++W  +  K+ +  G+             K +L+YG P    + +A  LA E+G  F+ +     M   +G SE     A+++IF  A     S+I  D+++ L
Sbjct:  355 IGGLNSQLKAIREIIELP-LKQPELFKSYGIPAPRGLLLYGPPGTGKTMIARAVANEVGAYV-SVINGPEIISKFYGETEARLRQIFAEATLRHP--------SIIFIDELDALCPKREGAQ--SEVEKRVVASLLTLMDGIGSEGSEGRVLVLGATNRPQALDAALRRPGRFDKEIEIGIPNAQDRLDILQ---KLLRRVPHLLTKAELLRLANNAHGYVGADLKALCNEAGLHALRRVLRKQPNLPDSKV--AGMVKITLNDFLQGMNDIRPSAMREVAIDVPNVSWSDIGGLENIKLKLKQAVEWPLKHPKSFNRMGIQ----------PPKGVLLYGPPGCSKTMIAKALANESGLNFLAIKGPELMNKYVGESE----RAVREIFRKARAVAPSIIFFDELDAL 732          
BLAST of Vesicle-fusing ATPase vs. UniProt/SwissProt
Match: sp|P46461|NSF1_DROME (Vesicle-fusing ATPase 1 OS=Drosophila melanogaster OX=7227 GN=comt PE=2 SV=1)

HSP 1 Score: 597.43 bits (1539), Expect = 0.000e+0
Identity = 305/679 (44.92%), Postives = 437/679 (64.36%), Query Frame = 2
Query:   11 FKVQKNSYDEFSYTNKIFFNPSDWALFKE--KFITVTTASNK-FIFKSDKDESVKKGCMTTNGIQRKWMYCGIGEVVECIPYSFQXXXXXXX-XXXXXDLFVKSASLKDPIDSDKLAVKYLERFHDTPVTKNQLLLFNWESINFEA-KIISIQGLDVKS------SKMNEIETGIIQSNTVFKFQRRPESKFMLSGRCKAENAAVCLINPNWDFNSIGVGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEKFDPKKGIAIRIEDLQSFKINRQHFIFAIDHDVKALYGRHDDDLQEFKENRLLKWSPQISKALSTAGLLIESLKTNDNISRKRLLIYGEPLTGTSSVAVHLAKETGFTFIRMCSMRNMIGFSEPARCLAIKKIFDDALKAPASVIILDDVERLIDYSSIGPRFSIFLVNNLIGMINCFVPKNKKMLIIGTTKHLSAMHELEFFSAFDKNCIKIPEIKTPSEIISV 2014
             K  K   DE S TN+   N  D   F E  K+  ++ A  + FIF  +K   V  G +  + +QRKW    I + +E  PY F     V   V  E D   K    ++P DSD++A +++ +F    +T  Q L+FN++        + S++ +D KS      + M  +  G I  NTV +F++   S   L G+ K +     +INP+WDF  +G+GGLD+EF+ IF+ AF SR+FPPE VE+LG KHV+GILLYGPPGTGKTL ARQIG MLNAREPKIVNGP ILDKYVGESEAN+R+LFA+AEEE K++G  S LHIIIFDEIDAICK RG+++G S V DTVVNQLL K+DGV+QLNNIL+IGMTNRRDMIDEALLRPGR+ VQ+E+ LP++ GR+QI  IHTK++RE N + DD+D KE+AALTKN+SGAE+E +V AA ++A +        + +  E ++  K+NR  F+ +++HD+K  +G   + L       ++ W   +S  L    L ++  K  ++     +L+ G P +G +++A  LAK + F F+++CS  +M+G++E A+CL I+KIFDDA ++  S I++D+VERL+DY SIGPR+S   +  L+ ++    PK +K+LI+ T+     + E+E  +AF  + + +P +  P  +++V
Sbjct:    5 LKATKCPTDELSLTNRAIVNVGD---FPEEIKYADISPAPGQHFIFALEKTVEVPSGYVGFSLVQRKWAMVSINQELEVRPYRFDASSDVITCVSFETDFLQKKTVSQEPYDSDQMAKEFIMQFAGMALTVGQSLVFNFKDKKLLGLAVKSLEAIDPKSLGEGKDTAMRNVRFGRILGNTVVQFEKAENSSLNLQGKSKGKVVRQSIINPDWDFGKMGIGGLDKEFNSIFRRAFASRVFPPELVEQLGCKHVKGILLYGPPGTGKTLMARQIGTMLNAREPKIVNGPQILDKYVGESEANVRRLFAEAEEEEKRLGPNSGLHIIIFDEIDAICKQRGSVAGNSGVHDTVVNQLLTKIDGVDQLNNILVIGMTNRRDMIDEALLRPGRLEVQMEISLPNEQGRVQILNIHTKRMREFNKINDDVDNKEIAALTKNFSGAELEGLVRAAQSSAMNRLIKADAKVTVDPEAMEKLKVNRDDFLHSLEHDIKPAFGTAQEILDNMLARGVINWGAPVSNLLEDGMLYVQQAKAPESSGLVSVLVAGAPNSGKTALAAQLAKMSDFPFVKVCSPEDMVGYTESAKCLHIRKIFDDAYRSMLSCIVVDNVERLLDYGSIGPRYSNMTLQALLVLLKKQPPKGRKLLILCTSSRREVLEEMEMLTAF-TSVLHVPNLSKPDHVLAV 679          
BLAST of Vesicle-fusing ATPase vs. UniProt/SwissProt
Match: sp|P54351|NSF2_DROME (Vesicle-fusing ATPase 2 OS=Drosophila melanogaster OX=7227 GN=Nsf2 PE=2 SV=2)

HSP 1 Score: 590.112 bits (1520), Expect = 0.000e+0
Identity = 320/739 (43.30%), Postives = 471/739 (63.73%), Query Frame = 2
Query:   23 KNSYDEFSYTNKIFFNPSDWALFKEKFITVTTASN-KFIFKSDK--DESVKKGCMTTNGIQRKWMYCGIGEVVECIPYSFQXXXXXXXXXX-XXDLFVKSASLKDPIDSDKLAVKYLERFHDTPVTKNQLLLFNWESINFEA-KIISIQGLDVKS-----SKMNEIETGIIQSNTVFKFQRRPESKFMLSGRCKAENAAVCLINPNWDFNSIGVGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEKFDPKKGIAIRIEDLQSFKINRQHFIFAIDHDVKALYGRHDDDLQEFKENRLLKWSPQISKALSTAGLLIESLKTNDNISRKRLLIYGEPLTGTSSVAVHLAKETGFTFIRMCSMRNMIGFSEPARCLAIKKIFDDALKAPASVIILDDVERLIDYSSIGPRFSIFLVNNLIGMINCFVPKNKKMLIIGTTKHLSAMHELEFFSAFDKNCIKIPEIKTPSEIISVXXXXXXXXXXXXXXXXNHLQMGDCSDLAIGIKNLMENCKDLKSVDPARRVDELLMKLKSSSEFQF 2209
            K   DE S TNK   N SD+   + K++ ++      +IF  +K     +  G +  + +QRKW    I + ++  PY F     +  ++  E D   K  + ++P DSD++A ++L +F   P+T  Q L+F ++   F    + +++ +D ++      K   +  G I  NTV +F++   S   L GR K +     +INP+WDF  +G+GGLD+EF+ IF+ AF SR+FPPE VE+LGIKHV+GILLYGPPGTGKTL ARQIG MLNAREPKIVNGP ILDKYVGESEANIR+LFA+AEEE K++G  S LHIIIFDEIDAICKARG+++G S V DTVVNQLLAK+DGVEQLNNIL+IGMTNRRDMIDEALLRPGR+ VQ+E+ LP++ GR+QI  IHTK++R+ N +A D+D  E+AA TKN+SGAE+E +V AA + A +        + +  E ++  ++ R  F+ A+D+D+K  +G   + L+      ++ W P +++ L    L ++  K  ++     +LI G P +G S++A +LA+ + F F+++CS  +M+GF+E A+CL I+KIFDDA ++  S I++D+VERL+DY  IGPR+S   +  L+ ++    PK +K+LI+ T+     + E+E  SAF  + + +  + TP  +++VL D DL+S  +LQ I   +       L IGIK L+     ++  +P +RV + L K++     + 
Sbjct:   13 KCPTDELSLTNKAIVNVSDFT-EEVKYVDISPGPGLHYIFALEKISGPELPLGHVGFSLVQRKWATLSINQEIDVRPYRFDASADIITLVSFETDFLQKKTTTQEPYDSDEMAKEFLMQFAGMPLTVGQTLVFQFKDKKFLGLAVKTLEAVDPRTVGDSLPKTRNVRFGRILGNTVVQFEKAENSVLNLQGRSKGKIVRQSIINPDWDFGKMGIGGLDKEFNAIFRRAFASRVFPPELVEQLGIKHVKGILLYGPPGTGKTLMARQIGTMLNAREPKIVNGPQILDKYVGESEANIRRLFAEAEEEEKRLGPNSGLHIIIFDEIDAICKARGSVAGNSGVHDTVVNQLLAKIDGVEQLNNILVIGMTNRRDMIDEALLRPGRLEVQMEISLPNEQGRVQILNIHTKRMRDFNKIASDVDNNEIAAKTKNFSGAELEGLVRAAQSTAMNRLIKADSKVHVDPEAMEKLRVTRADFLHALDNDIKPAFGAAQEMLENLLARGIINWGPPVTELLEDGMLSVQQAKATESSGLVSVLIEGAPNSGKSALAANLAQLSDFPFVKVCSPEDMVGFTESAKCLHIRKIFDDAYRSTLSCIVVDNVERLLDYGPIGPRYSNLTLQALLVLLKKQPPKGRKLLILCTSSRRDVLEEMEMLSAF-TSVLHVSNLSTPENVLAVLDDSDLFSPEELQSIARKMA---GKRLCIGIKKLLALIDMIRQSEPHQRVIKFLSKMEEEGGLEM 746          
BLAST of Vesicle-fusing ATPase vs. UniProt/SwissProt
Match: sp|Q9QUL6|NSF_RAT (Vesicle-fusing ATPase OS=Rattus norvegicus OX=10116 GN=Nsf PE=1 SV=1)

HSP 1 Score: 586.645 bits (1511), Expect = 0.000e+0
Identity = 294/661 (44.48%), Postives = 430/661 (65.05%), Query Frame = 2
Query:   35 DEFSYTNKIFFNPSDWALFKEKFITVTTASNKFIFKSDKDESVKKGCMTTNGIQRKWMYCGIGEVVECIPYSFQXXXXXXXXXXXXDLFVKSASL-KDPIDSDKLAVKYLERFHDTPVTKNQLLLFNWESINFEAKIISIQGLDVK-------SSKMNEIETGIIQSNTVFKFQRRPESKFMLSGRCKAENAAVCLINPNWDFNSIGVGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEKFDPKKGIAIRIEDLQSFKINRQHFIFAIDHDVKALYGRHDDDLQEFKENRLLKWSPQISKALSTAGLLIESLKTNDNISRKRLLIYGEPLTGTSSVAVHLAKETGFTFIRMCSMRNMIGFSEPARCLAIKKIFDDALKAPASVIILDDVERLIDYSSIGPRFSIFLVNNLIGMINCFVPKNKKMLIIGTTKHLSAMHELEFFSAFDKNCIKIPEIKT 1993
            DE S +N    N  D+    +  +  T+ ++K+IF      SV  GC+  +  QRKW    IG+ +E   YSF         +     F++  ++  +P D+DK+A +++++F+    +  Q L+F++    F   +  I+ +D         S K  +IE G++  N+   F++   S   L G+ K +     +INP+W+F  +G+GGLD+EFS IF+ AF SR+FPPE VE++G KHV+GILLYGPPG GKTL ARQIGKMLNAREPK+VNGP IL+KYVGESEANIRKLFADAEEE +++G  S LHIIIFDEIDAICK RG+++G + V DTVVNQLL+K+DGVEQLNNIL+IGMTNR D+IDEALLRPGR+ V++E+ LP + GRLQI  IHT ++R   +L+ D+D+KELA  TKN+SGAE+E +V AA + A +        + + +E  +S ++ R  F+ ++++D+K  +G + +D   +  N ++KW   +++ L    LL++  K +D      +L+ G P +G +++A  +A+E+ F FI++CS   MIGFSE A+C A+KKIFDDA K+  S +++DD+ERL+DY  IGPRFS  ++  L+ ++    P+ +K+LIIGTT     + E+E  +AF    I +P I T
Sbjct:   14 DELSLSNCAVVNEKDYQ-SGQHVMVRTSPNHKYIFTLRTHPSVVPGCIAFSLPQRKWAGLSIGQDIEVALYSFDKAKQCIGTMTIEIDFLQKKNIDSNPYDTDKMAAEFIQQFNHQAFSVGQQLVFSFNDKLFGLLVKDIEAMDPSILKGEPASGKRQKIEVGLVVGNSQVAFEKAENSSLNLIGKAKTKENRQSIINPDWNFEKMGIGGLDKEFSDIFRRAFASRVFPPEIVEQMGCKHVKGILLYGPPGCGKTLLARQIGKMLNAREPKVVNGPEILNKYVGESEANIRKLFADAEEEQRRLGANSGLHIIIFDEIDAICKQRGSMAGSTGVHDTVVNQLLSKIDGVEQLNNILVIGMTNRPDLIDEALLRPGRLEVKMEIGLPDEKGRLQILHIHTARMRGHQLLSADVDIKELAVETKNFSGAELEGLVRAAQSTAMNRHIKASTKVEVDMEKAESLQVTRGDFLASLENDIKPAFGTNQEDYASYIMNGIIKWGDPVTRVLDDGELLVQQTKNSDRTPLVSVLLEGPPHSGKTALAAKIAEESNFPFIKICSPDKMIGFSETAKCQAMKKIFDDAYKSQLSCVVVDDIERLLDYVPIGPRFSNLVLQALLVLLKKAPPQGRKLLIIGTTSRKDVLQEMEMLNAFS-TTIHVPNIAT 672          
BLAST of Vesicle-fusing ATPase vs. UniProt/SwissProt
Match: sp|P46460|NSF_MOUSE (Vesicle-fusing ATPase OS=Mus musculus OX=10090 GN=Nsf PE=1 SV=2)

HSP 1 Score: 586.645 bits (1511), Expect = 0.000e+0
Identity = 294/661 (44.48%), Postives = 431/661 (65.20%), Query Frame = 2
Query:   35 DEFSYTNKIFFNPSDWALFKEKFITVTTASNKFIFKSDKDESVKKGCMTTNGIQRKWMYCGIGEVVECIPYSFQXXXXXXXXXXXXDLFVKSASL-KDPIDSDKLAVKYLERFHDTPVTKNQLLLFNWESINFEAKIISIQGLDVK-------SSKMNEIETGIIQSNTVFKFQRRPESKFMLSGRCKAENAAVCLINPNWDFNSIGVGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEKFDPKKGIAIRIEDLQSFKINRQHFIFAIDHDVKALYGRHDDDLQEFKENRLLKWSPQISKALSTAGLLIESLKTNDNISRKRLLIYGEPLTGTSSVAVHLAKETGFTFIRMCSMRNMIGFSEPARCLAIKKIFDDALKAPASVIILDDVERLIDYSSIGPRFSIFLVNNLIGMINCFVPKNKKMLIIGTTKHLSAMHELEFFSAFDKNCIKIPEIKT 1993
            DE S +N    N  D+    +  +  T+ ++K+IF      SV  GC+  +  QRKW    IG+ +E   YSF         +     F++  ++  +P D+DK+A +++++F++   +  Q L+F++    F   +  I+ +D         S K  +IE G++  N+   F++   S   L G+ K +     +INP+W+F  +G+GGLD+EFS IF+ AF SR+FPPE VE++G KHV+GILLYGPPG GKTL ARQIGKMLNAREPK+VNGP IL+KYVGESEANIRKLFADAEEE +++G  S LHIIIFDEIDAICK RG+++G + V DTVVNQLL+K+DGVEQLNNIL+IGMTNR D+IDEALLRPGR+ V++E+ LP + GRLQI  IHT ++R   +L+ D+D+KELA  TKN+SGAE+E +V AA + A +        + + +E  +S ++ R  F+ ++++D+K  +G + +D   +  N ++KW   +++ L    LL++  K +D      +L+ G P +G +++A  +A+E+ F FI++CS   MIGFSE A+C A+KKIFDDA K+  S +++DD+ERL+DY  IGPRFS  ++  L+ ++    P+ +K+LIIGTT     + E+E  +AF    I +P I T
Sbjct:   14 DELSLSNCAVVNEKDFQ-SGQHVMVRTSPNHKYIFTLRTHPSVVPGCIAFSLPQRKWAGLSIGQDIEVALYSFDKAKQCIGTMTIEIDFLQKKNIDSNPYDTDKMAAEFIQQFNNQAFSVGQQLVFSFNDKLFGLLVKDIEAMDPSILKGEPASGKRQKIEVGLVVGNSQVAFEKAENSSLNLIGKAKTKENRQSIINPDWNFEKMGIGGLDKEFSDIFRRAFASRVFPPEIVEQMGCKHVKGILLYGPPGCGKTLLARQIGKMLNAREPKVVNGPEILNKYVGESEANIRKLFADAEEEQRRLGANSGLHIIIFDEIDAICKQRGSMAGSTGVHDTVVNQLLSKIDGVEQLNNILVIGMTNRPDLIDEALLRPGRLEVKMEIGLPDEKGRLQILHIHTARMRGHQLLSADVDIKELAVETKNFSGAELEGLVRAAQSTAMNRHIKASTKVEVDMEKAESLQVTRGDFLASLENDIKPAFGTNQEDYASYIMNGIIKWGDPVTRVLDDGELLVQQTKNSDRTPLVSVLLEGPPHSGKTALAAKIAEESNFPFIKICSPDKMIGFSETAKCQAMKKIFDDAYKSQLSCVVVDDIERLLDYVPIGPRFSNLVLQALLVLLKKAPPQGRKLLIIGTTSRKDVLQEMEMLNAFS-TTIHVPNIAT 672          
BLAST of Vesicle-fusing ATPase vs. UniProt/SwissProt
Match: sp|P18708|NSF_CRIGR (Vesicle-fusing ATPase OS=Cricetulus griseus OX=10029 GN=NSF PE=1 SV=1)

HSP 1 Score: 582.793 bits (1501), Expect = 0.000e+0
Identity = 293/661 (44.33%), Postives = 430/661 (65.05%), Query Frame = 2
Query:   35 DEFSYTNKIFFNPSDWALFKEKFITVTTASNKFIFKSDKDESVKKGCMTTNGIQRKWMYCGIGEVVECIPYSFQXXXXXXXXXXXXDLFVKSASL-KDPIDSDKLAVKYLERFHDTPVTKNQLLLFNWESINFEAKIISIQGLDVK-------SSKMNEIETGIIQSNTVFKFQRRPESKFMLSGRCKAENAAVCLINPNWDFNSIGVGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEKFDPKKGIAIRIEDLQSFKINRQHFIFAIDHDVKALYGRHDDDLQEFKENRLLKWSPQISKALSTAGLLIESLKTNDNISRKRLLIYGEPLTGTSSVAVHLAKETGFTFIRMCSMRNMIGFSEPARCLAIKKIFDDALKAPASVIILDDVERLIDYSSIGPRFSIFLVNNLIGMINCFVPKNKKMLIIGTTKHLSAMHELEFFSAFDKNCIKIPEIKT 1993
            DE S +N    +  D+    +  I  T+ ++K+IF      SV  G +  +  QRKW    IG+ +E   YSF         +     F++  ++  +P D+DK+A +++++F++   +  Q L+F++    F   +  I+ +D         S K  +IE G++  N+   F++   S   L G+ K +     +INP+W+F  +G+GGLD+EFS IF+ AF SR+FPPE VE++G KHV+GILLYGPPG GKTL ARQIGKMLNAREPK+VNGP IL+KYVGESEANIRKLFADAEEE +++G  S LHIIIFDEIDAICK RG+++G + V DTVVNQLL+K+DGVEQLNNIL+IGMTNR D+IDEALLRPGR+ V++E+ LP + GRLQI  IHT ++R   +L+ D+D+KELA  TKN+SGAE+E +V AA + A +        + + +E  +S ++ R  F+ ++++D+K  +G + +D   +  N ++KW   +++ L    LL++  K +D      +L+ G P +G +++A  +A+E+ F FI++CS   MIGFSE A+C A+KKIFDDA K+  S +++DD+ERL+DY  IGPRFS  ++  L+ ++    P+ +K+LIIGTT     + E+E  +AF    I +P I T
Sbjct:   14 DELSLSNCAVVSEKDYQ-SGQHVIVRTSPNHKYIFTLRTHPSVVPGSVAFSLPQRKWAGLSIGQEIEVALYSFDKAKQCIGTMTIEIDFLQKKNIDSNPYDTDKMAAEFIQQFNNQAFSVGQQLVFSFNDKLFGLLVKDIEAMDPSILKGEPASGKRQKIEVGLVVGNSQVAFEKAENSSLNLIGKAKTKENRQSIINPDWNFEKMGIGGLDKEFSDIFRRAFASRVFPPEIVEQMGCKHVKGILLYGPPGCGKTLLARQIGKMLNAREPKVVNGPEILNKYVGESEANIRKLFADAEEEQRRLGANSGLHIIIFDEIDAICKQRGSMAGSTGVHDTVVNQLLSKIDGVEQLNNILVIGMTNRPDLIDEALLRPGRLEVKMEIGLPDEKGRLQILHIHTARMRGHQLLSADVDIKELAVETKNFSGAELEGLVRAAQSTAMNRHIKASTKVEVDMEKAESLQVTRGDFLASLENDIKPAFGTNQEDYASYIMNGIIKWGDPVTRVLDDGELLVQQTKNSDRTPLVSVLLEGPPHSGKTALAAKIAEESNFPFIKICSPDKMIGFSETAKCQAMKKIFDDAYKSQLSCVVVDDIERLLDYVPIGPRFSNLVLQALLVLLKKAPPQGRKLLIIGTTSRKDVLQEMEMLNAFS-TTIHVPNIAT 672          
BLAST of Vesicle-fusing ATPase vs. TrEMBL
Match: C1LF61 (NEM-sensitive fusion protein 2 OS=Schistosoma japonicum OX=6182 GN=Nsf2 PE=2 SV=1)

HSP 1 Score: 612.453 bits (1578), Expect = 0.000e+0
Identity = 323/741 (43.59%), Postives = 464/741 (62.62%), Query Frame = 2
Query:   11 FKVQKNSYDEFSYTNKIFFNPSDWALFKEKF--ITVTTASNKFIFKSDKDESVKKGCMTTNGIQRKWMYCGIGEVVECIPYSFQXXXX-XXXXXXXXDLFVKSASLKDPIDSDKLAVKYLERFHDTPVTKNQLLLFNWESINFEAKIISIQGLDVKSSKMNEIETGIIQSNTVFKFQRRPESKFMLSGRCKAENAAVC----------LINPNWDFNSIGVGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEKFDPKKGIAIRIEDLQSFKINRQHFIFAIDHDVKALYGRHDDDLQEFKENRLLKWSPQISKALSTAGLLIESLKTNDNISRKRL--LIYGEPLTGTSSVAVHLAKETGFTFIRMCSMRNMIGFSEPARCLAIKKIFDDALKAPASVIILDDVERLIDYSSIGPRFSIFLVNNLIGMINCFVPKNKKMLIIGTTKHLSAMHELEFFSAFDKNCIKIPEIKTPSEIISVXXXXXXXXXXXXXXXXNHLQMGDCSDLAIGIKNLMENCKDLKSVDPARRVDELLMKLK 2188
            F   K   +E S TN++FFNP D   F +KF  + + T ++K+IF+    E+V +G +     QRKW    + E ++  PY+F      +   ++ +D   K  S+ +P+DSDK+A+++  +F DTP+T  QL+L+ +  I F  ++  +  L      ++  + G++  NTV  +   P+SK +L G+   E               +INPNWDFN +G+GGLD+EFS IF+  F SR+FPP   ++LG+KHVRGILLYGPPGTGKTL ARQIG MLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEE K+MG +SALHIIIFDEIDAICK RG+  GG+ V DTVVNQLL  +DGV QLNNIL+IGMTNRRDMIDEALLRPGR  +Q+E+ LP + GR QI  IHT K+++   LA D+DLKELAA+TKN+SGAEIE +  AA   +  +   P     +  +      + R  F++A++HDVK  +G  +++L  +    ++ W   +S AL    L + ++K  D  + + L  L+ G P  G +++AV +A+ +GF F+++ +   MIGF+E A+C A+KKIFDD  K+P SV+I+D++E LI+Y+ +GPRFS F+V  +  +++  +   + ML++ TT    A+ +     AF  + + +  +  P  II+ L +   ++  + Q I+  L       L IGIK+L++    +   D   RV   L KL+
Sbjct:    3 FFATKAPNEEVSLTNRVFFNPKD---FNDKFGCVAIHTTADKYIFRGGSHENVPQGKVAFGLAQRKWGNISVDEPLDVQPYTFNLARECLASAVLAVDFNNKKMSINEPVDSDKMALEFSMQFADTPLTVGQLILYRFNKILFLVEVKKLSSLSFDGGDVDNSKIGMLTGNTVIHWVSHPDSKLLLIGKAALEGDGATSFGGGGGYHSIINPNWDFNQMGIGGLDKEFSAIFRRTFASRMFPPAVAKQLGLKHVRGILLYGPPGTGKTLMARQIGTMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEEKRMGAKSALHIIIFDEIDAICKTRGSTGGGAGVHDTVVNQLLTNIDGVNQLNNILVIGMTNRRDMIDEALLRPGRFEMQMEISLPDEDGRFQILNIHTAKMQQAGKLARDVDLKELAAMTKNFSGAEIEGLCRAAAFTSMYQLISPSGKTQLDPDAFDRLLVKRSDFLYALEHDVKPAFGTSEEELSCYAPRGIMMWGESVSHALDMGRLAVSAVKEPDVETYRALTMLLEGPPKAGKTALAVEIARLSGFPFVKIVTSHKMIGFTEMAKCAAMKKIFDDGAKSPLSVVIVDNIEGLIEYNPVGPRFSNFIVQAIRDLVSQPLKGGRHMLVLATTSCREALTDQNLTQAFSWH-VHVGMLSKPEHIIAALEEDGRFTPKEQQYIEKSLS---GRRLCIGIKHLLDLIDLVSKNDANHRVAAFLAKLE 736          
BLAST of Vesicle-fusing ATPase vs. TrEMBL
Match: A0A0N4ZGZ3 (Uncharacterized protein OS=Parastrongyloides trichosuri OX=131310 PE=4 SV=1)

HSP 1 Score: 610.142 bits (1572), Expect = 0.000e+0
Identity = 337/747 (45.11%), Postives = 481/747 (64.39%), Query Frame = 2
Query:   11 FKVQKNSYDEFSYTNKIFFNPSDWALFKEKFITVTTA-SNKFIFKSDKDESVKKGCMTTNGIQRKWMYCGIGEVVECIPYSFQXXXXXXXXXXXXDLFVKSASLKDPIDSDKLAVKYLERFHDTPVTKNQLLLFNWESI------NFEAKIISIQGLDVKSS------KMNEIETGIIQSNTVFKFQRRPESKFMLSGRCKAENAAVCLINPNWDFNSIGVGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEKFDPKKGIAIRIEDLQSFKINRQHFIFAIDHDVKALYGRHDDDLQEFKENRLLKWSPQISKALSTAGLLIESLKTNDNISRKRLLIYGEPLTGTSSVAVHLAKETGFTFIRMCSMRNMIGFSEPARCLAIKKIFDDALKAPASVIILDDVERLIDYSSIGPRFSIFLVNNLIGMINCFVPKNKKMLIIGTTKHLSAMHELEFFSAFDKNCIKIPEIKTPSEIISVXXXXXXXXXXXXXXXXNHLQ-MGDCSDLAIGIKNLMENCKDLKSVDPARRVDELLMKLKSSSEFQF 2209
             KV+K   DE + +N    +  D+     K I V T  +++F F       + +G +      RKW    + + V+C P++     L+  + V++D F K     D +DSD ++ ++  +F     T+ QLL+F +++        F   +ISI G+DV ++        ++I  G +  N+V  F +R  ++  L G+ K + A   +I+P+WDF S+G+GGLD+EFS IF+ AF SRLFPPE VE+LG+KHVRGILLYGPPGTGKTL ARQIGKMLNAREPKIVNGP ILDKYVGESEANIRKLFADAEEEFK+ GN SALHIIIFDEIDAICK RG  +G S V DTVVNQ+L KMDGV+QLNNIL+IGMTNRRDMIDEALLRPGRM VQ+E+ LP ++GR+QI  IHT K+RE + L+ D+DLKELA  TKN+SGAE+E +V AA ++A +        + +  +  +  KI  + F +A+ +D+K  +G  D+ L+ F    +L WSP+ISK L T  + I   K+ D+     LL+ G    G S +A  +AK +GF F+++C+   M+G++E A+C A++KIFDDA ++P SVII+D+++RL+DY+S+GPR+S  ++  L+ ++    PK K++L++ T    S + EL+  SAFD + I +P +KT   I  VL D +++S+ D+Q I+  ++  GD     IGIK ++      K  DP  R    + K+ +++  Q+
Sbjct:    5 LKVRKVPTDELAVSNCAVVSRDDFDANGIKHIIVQTGPAHRFAFSIKNHPGISRGEIGFGVPARKWAALSLDQNVQCSPFTVPNNQLIETITVDIDFFTKKNVCNDTLDSDDMSREFSMQFSGQVFTEGQLLVFKYKNPKDGKEHTFSLNVISISGMDVNAAIGGGAPATSKIFFGQLMGNSVIIFDKREGAQINLVGKSKGKTAFKSIISPDWDFESMGIGGLDKEFSAIFRRAFFSRLFPPEQVEQLGMKHVRGILLYGPPGTGKTLIARQIGKMLNAREPKIVNGPQILDKYVGESEANIRKLFADAEEEFKRCGNNSALHIIIFDEIDAICKQRGTQAGSSGVHDTVVNQILTKMDGVDQLNNILVIGMTNRRDMIDEALLRPGRMEVQMEISLPDENGRVQILHIHTGKMREFDKLSPDVDLKELAKKTKNFSGAELEGLVRAAQSSAINRLVKVDGKVTVEADAFEKLKITSEDFDYALANDIKPAFGSADEVLERFLSGGILMWSPEISKILETGDIHINDAKSPDSRGFVTLLLAGSAGVGKSCLAAQIAKNSGFPFVKICTPETMVGYTEIAKCQALRKIFDDAYRSPLSVIIIDNIDRLLDYASVGPRYSNQVLQALLVLLGTKPPKGKRLLVLATASQRSFLKELDLMSAFD-DVIDVPTLKTVEHIRHVLEDSNVFSNQDIQSIERDIRAAGDI--FYIGIKKVLRIIDYTKQCDPRFRAAIFVEKIINNAFNQY 748          
BLAST of Vesicle-fusing ATPase vs. TrEMBL
Match: A0A0K0DX31 (Uncharacterized protein OS=Strongyloides stercoralis OX=6248 PE=4 SV=1)

HSP 1 Score: 609.757 bits (1571), Expect = 0.000e+0
Identity = 335/750 (44.67%), Postives = 478/750 (63.73%), Query Frame = 2
Query:    5 IAFKVQKNSYDEFSYTNKIFFNPSDWALFKEKFITVTTA-SNKFIFKSDKDESVKKGCMTTNGIQRKWMYCGIGEVVECIPYSFQXXXXXXXXXXXXDLFVKSASLKDPIDSDKLAVKYLERFHDTPVTKNQLLLFNWESI------NFEAKIISIQGLDVKSS-------KMNEIETGIIQSNTVFKFQRRPESKFMLSGRCKAENAAVCLINPNWDFNSIGVGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEKFDPKKGIAIRIEDLQSFKINRQHFIFAIDHDVKALYGRHDDDLQEFKENRLLKWSPQISKALSTAGLLIESLKTNDNISRKRLLIYGEPLTGTSSVAVHLAKETGFTFIRMCSMRNMIGFSEPARCLAIKKIFDDALKAPASVIILDDVERLIDYSSIGPRFSIFLVNNLIGMINCFVPKNKKMLIIGTTKHLSAMHELEFFSAFDKNCIKIPEIKTPSEIISVXXXXXXXXXXXXXXXXNHLQ-MGDCSDLAIGIKNLMENCKDLKSVDPARRVDELLMKLKSSSEFQF 2209
            I  KV+K   DE + +N    N  D+     K I V T  +++F F       V +G +      RKW    + + V+C P++     L+  + V++D F K     D +DSD ++ ++  +F     T+ Q L+F +++        F   +I I G+DV ++          +I  G +  N++  F +R  ++  L+G+ K + A   +I+P+WDF S+G+GGLD+EFS IF+ AF SRLFPPE VE+LG+KHVRGILLYGPPGTGKTL ARQIGKMLNAREPKIVNGP ILDKYVGESEANIRKLF DAEEEFK+ GN SALHIIIFDEIDAICK RG  +G S V DTVVNQ+L KMDGV+QLNNIL+IGMTNRRDMIDEALLRPGRM VQ+E+ LP ++GR+QI  IHT K+RE + L  D+DLK +A  TKN+SGAEIE +V AA ++A +        + +  +  +  KI  + F +A+ +D+K  +G  D+ L+ F    +L WSP+ISK L T  + I   K+ D+     LL+ G P  G S +A  +AK +GF F+++C+   M+G++E A+C A++KIF+DA ++P SVII+D+++RL+DY+S+GPRFS  ++  L+ ++    PK K++L++ T    + + EL+  +AFD + I +P ++T   I  VL D +++S  D+Q I+  L+  GD   + IGIK ++      K  DP  R    + K+ +S+  Q+
Sbjct:    3 IVLKVRKVPTDELAVSNCAVVNRDDFDSNSIKHIIVQTGPAHRFAFSIKNHPGVSRGEIGFGVPARKWAALSLDQNVQCSPFTVPNNQLIESITVDIDFFTKKGVCNDVLDSDDMSREFSMQFSGQVFTEGQQLVFKYQNPKDKKEHTFSLNVIVITGMDVNAAIGGGGGESTGKIYFGQLMGNSMIIFDKREGAQLNLTGKSKGKTAFKSIISPDWDFESMGIGGLDKEFSAIFRRAFFSRLFPPEQVEQLGMKHVRGILLYGPPGTGKTLIARQIGKMLNAREPKIVNGPQILDKYVGESEANIRKLFGDAEEEFKRCGNNSALHIIIFDEIDAICKQRGTQAGSSGVHDTVVNQILTKMDGVDQLNNILVIGMTNRRDMIDEALLRPGRMEVQMEISLPDENGRVQILHIHTAKMREFDKLGSDVDLKVIAKKTKNFSGAEIEGLVRAAQSSAINRLVKVDGKVTVEADAFEKLKITAEDFEYALLNDIKPAFGSADEVLERFLSGGILMWSPEISKILETGDIHINDAKSPDSRGFVTLLLAGSPGVGKSCLAAQIAKNSGFPFVKICTPETMVGYTEIAKCQALRKIFEDAYRSPLSVIIIDNIDRLLDYASVGPRFSNQVLQALLVLLGTKPPKGKRLLVLATASQRTFLKELDLMAAFD-DVIDVPMLRTVEHIRHVLDDSNVFSPQDIQSIERDLRSAGDV--MYIGIKKILRIIDYTKQCDPRFRASTFVEKVINSAFNQY 749          
BLAST of Vesicle-fusing ATPase vs. TrEMBL
Match: G4VMW9 (Putative vesicular-fusion protein nsf OS=Schistosoma mansoni OX=6183 GN=Smp_057320 PE=4 SV=1)

HSP 1 Score: 609.757 bits (1571), Expect = 0.000e+0
Identity = 320/741 (43.18%), Postives = 469/741 (63.29%), Query Frame = 2
Query:   11 FKVQKNSYDEFSYTNKIFFNPSDWALFKEKF--ITVTTASNKFIFKSDKDESVKKGCMTTNGIQRKWMYCGIGEVVECIPYSFQXXXX-XXXXXXXXDLFVKSASLKDPIDSDKLAVKYLERFHDTPVTKNQLLLFNWESINFEAKIISIQGLDVKSSKMNEIETGIIQSNTVFKFQRRPESKFMLSGRCKAEN----------AAVCLINPNWDFNSIGVGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEKFDPKKGIAIRIEDLQSFKINRQHFIFAIDHDVKALYGRHDDDLQEFKENRLLKWSPQISKALSTAGLLIESLKTNDNISRK--RLLIYGEPLTGTSSVAVHLAKETGFTFIRMCSMRNMIGFSEPARCLAIKKIFDDALKAPASVIILDDVERLIDYSSIGPRFSIFLVNNLIGMINCFVPKNKKMLIIGTTKHLSAMHELEFFSAFDKNCIKIPEIKTPSEIISVXXXXXXXXXXXXXXXXNHLQMGDCSDLAIGIKNLMENCKDLKSVDPARRVDELLMKLK 2188
            F   K   +E S TN++FFNP D   F EK   + + TA++K++F+    + V +G +     QRKW    + E ++  PY+F      +   ++ +D   K  S+ +P+DSD++A+++  +F DTP+T  Q +L+ +  +    ++  +  L +    ++  + G++  NTV ++  +P+SK +L G+   ++              +INPNWDFN +G+GGLD+EFS IF+  F SR+FPP   ++LG+KHVRGILLYGPPGTGKTL ARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEE K+MG +SALHIIIFDEIDAICK+RG+  GG+ V DTVVNQLL  MDGV QLNNIL+IGMTNRRDMIDEALLRPGR  +Q+E+ LP + GRLQI  IHT K+++   LA D+DLKELAA TKN+SGAEIE +  AA   +  +   P     +  +      + R  F++A++HDVK  +G  +++L  +    ++ W   +S AL    L + ++K  D  + +   LL+ G P  G +++AV +A+ +GF F+++ +   MIGF+E A+C A+KKIFDD  K+P SV+I+D++E LI+Y+ +GPRFS F+V  +  +++  +   ++ML++ TT    A+ +     AF  + + +  +  P  +I+ L +   ++  + Q I+  L       L IGIK+L++    +   DP  RV   L KL+
Sbjct:    3 FFAAKAPNEEVSLTNRVFFNPKD---FNEKLSCVAIHTAADKYVFRGGSHDHVPQGKVAFGLAQRKWGNISVDEPLDVQPYTFNLARECLASAVLAVDFNNKKMSINEPLDSDRMALEFSMQFADTPLTVGQHILYRFNKMTLLVEVKKLSTLSMDGGDVDNSKIGMLTGNTVIRWMPQPDSKLLLIGKAALDDDGASSIGGGGGYHSIINPNWDFNQMGIGGLDKEFSAIFRRTFASRMFPPAVAKQLGLKHVRGILLYGPPGTGKTLMARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEEKRMGAKSALHIIIFDEIDAICKSRGSTGGGAGVHDTVVNQLLTNMDGVNQLNNILVIGMTNRRDMIDEALLRPGRFEMQMEISLPDEDGRLQILNIHTSKMQQAGKLARDVDLKELAAKTKNFSGAEIEGLCRAAAFTSMYQLISPSGKTQLDPDAFDRLLVKRSDFLYALEHDVKPAFGASEEELSCYAPRGIMMWGESVSHALDMGRLAVSAVKEPDVETYRPLTLLLEGPPKAGKTALAVEIARLSGFPFVKIVTSHKMIGFTEMAKCAAMKKIFDDGAKSPLSVVIVDNIEGLIEYNPVGPRFSNFIVQAIRDLVSQPLKAGRRMLVLATTSCREALTDQNLTQAFSWH-VHVAMLSRPEHLIAALEEDGRFTSKEQQIIEKSLS---GRRLCIGIKHLLDLIDLVSKNDPDHRVAAFLAKLE 736          
BLAST of Vesicle-fusing ATPase vs. TrEMBL
Match: A0A267EGJ4 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig016436g3 PE=4 SV=1)

HSP 1 Score: 609.372 bits (1570), Expect = 0.000e+0
Identity = 315/738 (42.68%), Postives = 474/738 (64.23%), Query Frame = 2
Query:    2 MIAFKVQKNSYDEFSYTNKIFFNPSDWALFKEKFITVTTASNKFIFKSDKDESVKKGCMTTNGIQRKWMYCGIGEVVEC-IPYSFQXXXX-XXXXXXXXDLFVKSASLKDPIDSDKLAVKYLERFHDTPVTKNQLLLFNWESINFEAKIISIQGLDVKSSKMNEI--ETGIIQSNTVFKFQRRPESKFMLSGRCKAENAAVCLINPNWDFNSIGVGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEKFDPKKGIAIRIEDLQSFKINRQHFIFAIDHDVKALYGRHDDDLQEFKENRLLKWSPQISKALSTAGLL------IESLKTNDNISRKRLLIYGEPLTGTSSVAVHLAKETGFTFIRMCSMRNMIGFSEPARCLAIKKIFDDALKAPASVIILDDVERLIDYSSIGPRFSIFLVNNLIGMINCFVPKNKKMLIIGTTKHLSAMHELEFFSAFDKNCIKIPEIKTPSEIISVXXXXXXXXXXXXXXXXNHLQMGDCSDLAIGIKNLMENCKDLKSVDPARRVDELLMKL 2185
            M  F+  K   DE S TNKIFFNP+D    K +FI + + S  + F  D + S+  G    + +QR+W    IG+ V      SF      +  V +E D  +K ++  DP +SD++ +++ + F   P+T  Q ++F ++  + +  ++ ++ ++    + + +  + G+I  NT+  F++ P+S  +L+G+ KA+     +INP+W+F+ +G+GGLD EFS IF+  F SR+FPP+ VE+LG+ HVRG+LLYGPPGTGKTL ARQIGKMLNAREPKIVNGPSILDKYVGESEA IR+LFADAEEEFK++GN SALHIIIFDEIDAICKARG++ GG+ V DTVVNQLL+K+DGVE LNNIL+IGMTNR+DMIDEALLRPGR  VQ+E+ LP +HGR QI  IHT K+R+   L+ D+D++ELAA TKN+SGAE+  +V AA   A +      + + +  + ++   + R  F+ A+++D++  +G  +++L  +    L++W   ++  +   GLL       E+    D+     LL+ G   +G +++AVH+A++ GF+++R+ + +NM+GF+E A+C+A+KK FDDA KA  +V+ILD +E LIDYS +GPR+S +++  L  +I   +P+NK +L++GTT    A+ EL     F    I +  +  P  +++ L   D    ++ +    +  +  C  L IGIK L++  +    V+   RV   L +L
Sbjct:    5 MQNFEATKCPSDELSLTNKIFFNPNDVRSLKNQFIKIHSGSGSYAFLLDSNPSIPTGKAGFSLVQRRWANVSIGQPVSVEFNCSFDPRTTCIATVTLEADFLLKKSTTTDPYNSDEMLIEFTQCFPQMPLTVGQEIVFKFK--DKKHLLLRVKEMETLQPRGDVVAAQFGVISGNTIATFEKLPDSPLVLTGKAKAKQPYQSIINPDWNFSMMGIGGLDREFSDIFRRTFASRIFPPDLVEQLGMCHVRGLLLYGPPGTGKTLMARQIGKMLNAREPKIVNGPSILDKYVGESEAKIRQLFADAEEEFKRVGNNSALHIIIFDEIDAICKARGSVGGGTQVHDTVVNQLLSKIDGVETLNNILVIGMTNRKDMIDEALLRPGRFEVQMEISLPDEHGRHQILSIHTSKIRDNGKLSPDVDVRELAAKTKNFSGAELAGLVRAATVTAMNRLVQASEKVQVDPDAIEKLCVTRHDFLHALEYDIRPSFGAQEEELDRYVTGGLVQWGNPVNHVIE-HGLLATRAARAEAEAGADSGRPVALLLEGPRNSGKTALAVHIARQAGFSYVRLATSKNMVGFNEVAKCMAVKKFFDDAHKAKQAVVILDGLEGLIDYSPVGPRYSNYVLQALRDLITAPLPRNKCLLLLGTTSCRDALEELGLVQCFTAGKIHVSNLTQPEHLVTALNQLDAAKFTEKEVRQIYKSVSGCR-LNIGIKELLDVARMTNRVEEGDRVRYFLDRL 738          
BLAST of Vesicle-fusing ATPase vs. Ensembl Cavefish
Match: nsfa (vesicle-fusing ATPase [Source:NCBI gene;Acc:103037152])

HSP 1 Score: 573.548 bits (1477), Expect = 0.000e+0
Identity = 296/667 (44.38%), Postives = 434/667 (65.07%), Query Frame = 2
Query:   35 DEFSYTNKIFFNPSDWALFKEKFITV-TTASNKFIFKSDKDESVKKGCMTTNGIQRKWMYCGIGEVVECIPYSF-QXXXXXXXXXXXXDLFVKSASLKDPIDSDKLAVKYLERFHDTPVTKNQLLLFNWESINFEAKIISIQGLDVK-------SSKMNEIETGIIQSNTVFKFQRRPESKFMLSGRCKAENAAVCLINPNWDFNSIGVGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEKFDPKKGIAIRIEDLQSFKINRQHFIFAIDHDVKALYGRHDDDLQEFKENRLLKWSPQISKALSTAGLLIESLKTNDNISRKRLLIYGEPLTGTSSVAVHLAKETGFTFIRMCSMRNMIGFSEPARCLAIKKIFDDALKAPASVIILDDVERLIDYSSIGPRFSIFLVNNLIGMINCFVPKNKKMLIIGTTKHLSAMHELEFFSAFDKNCIKIPEIKTPSEII 2008
            DE S TN    +  D  L   + +TV TT ++KF+F      +V  G +  +  QRKW    IG+ VE   Y+F ++   +  + +E+D   K ++   P DSDK+A +++++F++   + +Q L+F++    F   +  ++ +D         S K  +IE G++  N+   F++   S   L G+ K + A   +INP+W+F  +G+GGLD+EFS IF+ AF SR+FPP+ VE++G KHV+GILL+GPPG GKTL ARQIGKMLNAREPKIVNGP IL+KYVGESEANIRKLFADAE+E K++G  S LHIIIFDE+DAICK RG  +  + V DTVVNQLL+K+DGVEQLNNIL+IGMTNR D+ID+AL+RPGR  V++E+ LP + GR+QI  IHT K+RE N+LA D++LKELAA TKNYSGAE+E +V AA + A +        + + +E  +  ++ R  F+ ++++D+K  +G + +D   +  N ++KW   ++  L    LL++  K +D      +L+ G P +G +++A  +A+++ F FI++CS   MIG SE  +C AIKKIFDDA K+  S +++DD+ERL+DY  IGPRFS  ++  L+ ++    P+ +K+LIIGTT     + E+E   AF    I IP I +   ++
Sbjct:   20 DELSLTNCAVVSEKD--LQSGQHVTVKTTPNHKFVFTVKSHHTVAPGSIAFSLPQRKWAGLSIGQEVEVANYNFDKSKQCIGAMTIEIDFLQKKSTDSSPYDSDKMASEFIQQFNNQAFSVSQQLVFSFCDKLFGLVVKDMEAMDPSILKGEPASGKKQKIEIGLLVGNSQVIFEKAESSSLTLVGKAKTKEARQTIINPDWNFERMGIGGLDKEFSDIFRRAFASRVFPPDIVEQMGCKHVKGILLFGPPGCGKTLMARQIGKMLNAREPKIVNGPEILNKYVGESEANIRKLFADAEDEQKRLGANSGLHIIIFDELDAICKQRGTGASSTGVHDTVVNQLLSKIDGVEQLNNILVIGMTNRPDLIDDALMRPGRFEVKMEIGLPDEKGRVQILSIHTAKMREFNLLAIDVNLKELAAETKNYSGAELEGLVRAAQSTAMNRHIKATATVEVDLEKAEKLQVTRDDFMGSLNNDIKPAFGTNQEDYASYIMNGIIKWGDPVTHVLDDGELLVQQTKNSDRTPLVAVLLEGPPHSGKTALAAKIAEDSQFPFIKICSPDKMIGHSEITKCQAIKKIFDDAYKSQLSCVVVDDIERLLDYVPIGPRFSNLVLQALLVLLKKAPPQGRKLLIIGTTSRKDVLQEMEMLDAF-STTIHIPNISSGEHLV 683          
BLAST of Vesicle-fusing ATPase vs. Ensembl Cavefish
Match: nsfa (vesicle-fusing ATPase [Source:NCBI gene;Acc:103037152])

HSP 1 Score: 573.163 bits (1476), Expect = 0.000e+0
Identity = 296/667 (44.38%), Postives = 434/667 (65.07%), Query Frame = 2
Query:   35 DEFSYTNKIFFNPSDWALFKEKFITV-TTASNKFIFKSDKDESVKKGCMTTNGIQRKWMYCGIGEVVECIPYSF-QXXXXXXXXXXXXDLFVKSASLKDPIDSDKLAVKYLERFHDTPVTKNQLLLFNWESINFEAKIISIQGLDVK-------SSKMNEIETGIIQSNTVFKFQRRPESKFMLSGRCKAENAAVCLINPNWDFNSIGVGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEKFDPKKGIAIRIEDLQSFKINRQHFIFAIDHDVKALYGRHDDDLQEFKENRLLKWSPQISKALSTAGLLIESLKTNDNISRKRLLIYGEPLTGTSSVAVHLAKETGFTFIRMCSMRNMIGFSEPARCLAIKKIFDDALKAPASVIILDDVERLIDYSSIGPRFSIFLVNNLIGMINCFVPKNKKMLIIGTTKHLSAMHELEFFSAFDKNCIKIPEIKTPSEII 2008
            DE S TN    +  D  L   + +TV TT ++KF+F      +V  G +  +  QRKW    IG+ VE   Y+F ++   +  + +E+D   K ++   P DSDK+A +++++F++   + +Q L+F++    F   +  ++ +D         S K  +IE G++  N+   F++   S   L G+ K + A   +INP+W+F  +G+GGLD+EFS IF+ AF SR+FPP+ VE++G KHV+GILL+GPPG GKTL ARQIGKMLNAREPKIVNGP IL+KYVGESEANIRKLFADAE+E K++G  S LHIIIFDE+DAICK RG  +  + V DTVVNQLL+K+DGVEQLNNIL+IGMTNR D+ID+AL+RPGR  V++E+ LP + GR+QI  IHT K+RE N+LA D++LKELAA TKNYSGAE+E +V AA + A +        + + +E  +  ++ R  F+ ++++D+K  +G + +D   +  N ++KW   ++  L    LL++  K +D      +L+ G P +G +++A  +A+++ F FI++CS   MIG SE  +C AIKKIFDDA K+  S +++DD+ERL+DY  IGPRFS  ++  L+ ++    P+ +K+LIIGTT     + E+E   AF    I IP I +   ++
Sbjct:   14 DELSLTNCAVVSEKD--LQSGQHVTVKTTPNHKFVFTVKSHHTVAPGSIAFSLPQRKWAGLSIGQEVEVANYNFDKSKQCIGAMTIEIDFLQKKSTDSSPYDSDKMASEFIQQFNNQAFSVSQQLVFSFCDKLFGLVVKDMEAMDPSILKGEPASGKKQKIEIGLLVGNSQVIFEKAESSSLTLVGKAKTKEARQTIINPDWNFERMGIGGLDKEFSDIFRRAFASRVFPPDIVEQMGCKHVKGILLFGPPGCGKTLMARQIGKMLNAREPKIVNGPEILNKYVGESEANIRKLFADAEDEQKRLGANSGLHIIIFDELDAICKQRGTGASSTGVHDTVVNQLLSKIDGVEQLNNILVIGMTNRPDLIDDALMRPGRFEVKMEIGLPDEKGRVQILSIHTAKMREFNLLAIDVNLKELAAETKNYSGAELEGLVRAAQSTAMNRHIKATATVEVDLEKAEKLQVTRDDFMGSLNNDIKPAFGTNQEDYASYIMNGIIKWGDPVTHVLDDGELLVQQTKNSDRTPLVAVLLEGPPHSGKTALAAKIAEDSQFPFIKICSPDKMIGHSEITKCQAIKKIFDDAYKSQLSCVVVDDIERLLDYVPIGPRFSNLVLQALLVLLKKAPPQGRKLLIIGTTSRKDVLQEMEMLDAF-STTIHIPNISSGEHLV 677          
BLAST of Vesicle-fusing ATPase vs. Ensembl Cavefish
Match: nsfa (vesicle-fusing ATPase [Source:NCBI gene;Acc:103037152])

HSP 1 Score: 573.163 bits (1476), Expect = 0.000e+0
Identity = 296/667 (44.38%), Postives = 434/667 (65.07%), Query Frame = 2
Query:   35 DEFSYTNKIFFNPSDWALFKEKFITV-TTASNKFIFKSDKDESVKKGCMTTNGIQRKWMYCGIGEVVECIPYSF-QXXXXXXXXXXXXDLFVKSASLKDPIDSDKLAVKYLERFHDTPVTKNQLLLFNWESINFEAKIISIQGLDVK-------SSKMNEIETGIIQSNTVFKFQRRPESKFMLSGRCKAENAAVCLINPNWDFNSIGVGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEKFDPKKGIAIRIEDLQSFKINRQHFIFAIDHDVKALYGRHDDDLQEFKENRLLKWSPQISKALSTAGLLIESLKTNDNISRKRLLIYGEPLTGTSSVAVHLAKETGFTFIRMCSMRNMIGFSEPARCLAIKKIFDDALKAPASVIILDDVERLIDYSSIGPRFSIFLVNNLIGMINCFVPKNKKMLIIGTTKHLSAMHELEFFSAFDKNCIKIPEIKTPSEII 2008
            DE S TN    +  D  L   + +TV TT ++KF+F      +V  G +  +  QRKW    IG+ VE   Y+F ++   +  + +E+D   K ++   P DSDK+A +++++F++   + +Q L+F++    F   +  ++ +D         S K  +IE G++  N+   F++   S   L G+ K + A   +INP+W+F  +G+GGLD+EFS IF+ AF SR+FPP+ VE++G KHV+GILL+GPPG GKTL ARQIGKMLNAREPKIVNGP IL+KYVGESEANIRKLFADAE+E K++G  S LHIIIFDE+DAICK RG  +  + V DTVVNQLL+K+DGVEQLNNIL+IGMTNR D+ID+AL+RPGR  V++E+ LP + GR+QI  IHT K+RE N+LA D++LKELAA TKNYSGAE+E +V AA + A +        + + +E  +  ++ R  F+ ++++D+K  +G + +D   +  N ++KW   ++  L    LL++  K +D      +L+ G P +G +++A  +A+++ F FI++CS   MIG SE  +C AIKKIFDDA K+  S +++DD+ERL+DY  IGPRFS  ++  L+ ++    P+ +K+LIIGTT     + E+E   AF    I IP I +   ++
Sbjct:   20 DELSLTNCAVVSEKD--LQSGQHVTVKTTPNHKFVFTVKSHHTVAPGSIAFSLPQRKWAGLSIGQEVEVANYNFDKSKQCIGAMTIEIDFLQKKSTDSSPYDSDKMASEFIQQFNNQAFSVSQQLVFSFCDKLFGLVVKDMEAMDPSILKGEPASGKKQKIEIGLLVGNSQVIFEKAESSSLTLVGKAKTKEARQTIINPDWNFERMGIGGLDKEFSDIFRRAFASRVFPPDIVEQMGCKHVKGILLFGPPGCGKTLMARQIGKMLNAREPKIVNGPEILNKYVGESEANIRKLFADAEDEQKRLGANSGLHIIIFDELDAICKQRGTGASSTGVHDTVVNQLLSKIDGVEQLNNILVIGMTNRPDLIDDALMRPGRFEVKMEIGLPDEKGRVQILSIHTAKMREFNLLAIDVNLKELAAETKNYSGAELEGLVRAAQSTAMNRHIKATATVEVDLEKAEKLQVTRDDFMGSLNNDIKPAFGTNQEDYASYIMNGIIKWGDPVTHVLDDGELLVQQTKNSDRTPLVAVLLEGPPHSGKTALAAKIAEDSQFPFIKICSPDKMIGHSEITKCQAIKKIFDDAYKSQLSCVVVDDIERLLDYVPIGPRFSNLVLQALLVLLKKAPPQGRKLLIIGTTSRKDVLQEMEMLDAF-STTIHIPNISSGEHLV 683          
BLAST of Vesicle-fusing ATPase vs. Ensembl Cavefish
Match: nsfb (N-ethylmaleimide-sensitive factor b [Source:ZFIN;Acc:ZDB-GENE-050808-1])

HSP 1 Score: 568.155 bits (1463), Expect = 0.000e+0
Identity = 289/665 (43.46%), Postives = 428/665 (64.36%), Query Frame = 2
Query:   35 DEFSYTNKIFFNPSDWALFKEKFITVTTASNKFIFKSDKDESVKKGCMTTNGIQRKWMYCGIGEVVECIPYSFQXXXXXX-XXXXXXDLFVKSASLKDPIDSDKLAVKYLERFHDTPVTKNQLLLFNWESINFEAKIISIQGLDV------KSSKMNEIETGIIQSNTVFKFQRRPESKFMLSGRCKAENAAVCLINPNWDFNSIGVGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEKFDPKKGIAIRIEDLQSFKINRQHFIFAIDHDVKALYGRHDDDLQEFKENRLLKWSPQISKALSTAGLLIESLKTNDNISRKRLLIYGEPLTGTSSVAVHLAKETGFTFIRMCSMRNMIGFSEPARCLAIKKIFDDALKAPASVIILDDVERLIDYSSIGPRFSIFLVNNLIGMINCFVPKNKKMLIIGTTKHLSAMHELEFFSAFDKNCIKIPEIKTPSEII 2008
            D+ S TN +  N  D    +   I  TT ++K++F     +SV  G +  +  QRKW    + + V+   Y+F T       + +E+D   K  +  +P D+DK+++++++ F+       Q  +F++    F   I  ++ +D       +SSK  +I+ G++  N+   F++   S   L G+ K   A   +INP+W+F  +G+GGLD+EFS IF+ AF SR+FPP+ VE++G KHV+GILL+GPPG GKTL ARQIGKMLNAREPK+VNGP IL+KYVGESEANIRKLFADAE+E K++G  S LHIIIFDEIDAICK RG+++G + V DTVVNQLL+K+DGVEQLNNIL+IGMTNR D+IDEALLRPGR+ V++E+ LP + GR+QI  IHT ++R+ N+L  D+D+KELA  TKNYSGAE+E +V AA + A +        + +  E  Q  +++R  F  ++++D+K  +G + +D   +  N ++KWS  ++  L    LL++  K ++      +L+ G P  G +++A  +A+++ F FI++CS   MIGFSE A+C AIKKIFDDA K+  S +++DD+ERL+D+  IGPRFS  ++  L+ ++    P+ +K+LI+GTT     M E+    +F    I IP I     ++
Sbjct:   11 DDLSLTNCVVANERDLKSGQHVMIR-TTPTHKYVFTVKTHQSVVSGTIAFSLPQRKWAGLALNQDVQVATYTFDTSRQYVGTMTLEVDFLQKKNADSNPYDTDKMSIEFIQFFNSQAFCVGQQFVFSFCEKLFLLVIKDVEAMDPSILRGEQSSK--KIDIGLLHGNSQVIFEKAESSTINLIGKSKTRGARQSIINPDWNFERMGIGGLDKEFSDIFRRAFASRVFPPDLVEQMGGKHVKGILLFGPPGCGKTLMARQIGKMLNAREPKVVNGPEILNKYVGESEANIRKLFADAEDEQKRLGVNSGLHIIIFDEIDAICKQRGSMAGSTGVHDTVVNQLLSKIDGVEQLNNILVIGMTNRPDLIDEALLRPGRLEVKMEIGLPDESGRVQILNIHTARMRQSNMLGTDVDIKELAVETKNYSGAELEGLVRAAQSTAMNRHIKASTKVEVDTEKAQELQVSRSDFWASLNNDIKPAFGTNQEDYSSYILNGIVKWSNAVTDILENGELLVQQTKNSERTPLVTVLLEGPPHCGKTALAAKIAEDSEFPFIKICSPDKMIGFSEIAKCQAIKKIFDDAYKSQLSCVLVDDIERLLDFVPIGPRFSNSVLQALLVLLKKTPPRGRKLLILGTTSRKDVMQEMGMLDSF-STTIHIPNISRGEHLV 671          
BLAST of Vesicle-fusing ATPase vs. Ensembl Cavefish
Match: nsfa (vesicle-fusing ATPase [Source:NCBI gene;Acc:103037152])

HSP 1 Score: 561.992 bits (1447), Expect = 0.000e+0
Identity = 295/675 (43.70%), Postives = 433/675 (64.15%), Query Frame = 2
Query:   35 DEFSYTNKIFFNPSDWALFKEKFITV-TTASNKFIFKSDKDESVKKGCMTTNGIQRKWMYCGIGEVVECIPYSF-QXXXXXXXXXXXXDLFVKSASLKDPIDSDKLAVKYLERFHDTPVTKNQLLLFNWESINFEAKIISIQGLDVK-------SSKMNEIETGIIQSNTVFKFQRRPESKFMLSGRCKAENAAVCLINPNWDFNSIGVGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQS--------ALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEKFDPKKGIAIRIEDLQSFKINRQHFIFAIDHDVKALYGRHDDDLQEFKENRLLKWSPQISKALSTAGLLIESLKTNDNISRKRLLIYGEPLTGTSSVAVHLAKETGFTFIRMCSMRNMIGFSEPARCLAIKKIFDDALKAPASVIILDDVERLIDYSSIGPRFSIFLVNNLIGMINCFVPKNKKMLIIGTTKHLSAMHELEFFSAFDKNCIKIPEIKTPSEII 2008
            DE S TN    +  D  L   + +TV TT ++KF+F      +V  G +  +  QRKW    IG+ VE   Y+F ++   +  + +E+D   K ++   P DSDK+A +++++F++   + +Q L+F++    F   +  ++ +D         S K  +IE G++  N+   F++   S   L G+ K + A   +INP+W+F  +G+GGLD+EFS IF+ AF SR+FPP+ VE++G KHV+GILL+GPPG GKTL ARQIGKMLNAREPKIVNGP IL+KYVGESEANIRKLFADAE+E K++ N           LHIIIFDE+DAICK RG  +  + V DTVVNQLL+K+DGVEQLNNIL+IGMTNR D+ID+AL+RPGR  V++E+ LP + GR+QI  IHT K+RE N+LA D++LKELAA TKNYSGAE+E +V AA + A +        + + +E  +  ++ R  F+ ++++D+K  +G + +D   +  N ++KW   ++  L    LL++  K +D      +L+ G P +G +++A  +A+++ F FI++CS   MIG SE  +C AIKKIFDDA K+  S +++DD+ERL+DY  IGPRFS  ++  L+ ++    P+ +K+LIIGTT     + E+E   AF    I IP I +   ++
Sbjct:    9 DELSLTNCAVVSEKD--LQSGQHVTVKTTPNHKFVFTVKSHHTVAPGSIAFSLPQRKWAGLSIGQEVEVANYNFDKSKQCIGAMTIEIDFLQKKSTDSSPYDSDKMASEFIQQFNNQAFSVSQQLVFSFCDKLFGLVVKDMEAMDPSILKGEPASGKKQKIEIGLLVGNSQVIFEKAESSSLTLVGKAKTKEARQTIINPDWNFERMGIGGLDKEFSDIFRRAFASRVFPPDIVEQMGCKHVKGILLFGPPGCGKTLMARQIGKMLNAREPKIVNGPEILNKYVGESEANIRKLFADAEDEQKRVRNIHTIQISSIFGLHIIIFDELDAICKQRGTGASSTGVHDTVVNQLLSKIDGVEQLNNILVIGMTNRPDLIDDALMRPGRFEVKMEIGLPDEKGRVQILSIHTAKMREFNLLAIDVNLKELAAETKNYSGAELEGLVRAAQSTAMNRHIKATATVEVDLEKAEKLQVTRDDFMGSLNNDIKPAFGTNQEDYASYIMNGIIKWGDPVTHVLDDGELLVQQTKNSDRTPLVAVLLEGPPHSGKTALAAKIAEDSQFPFIKICSPDKMIGHSEITKCQAIKKIFDDAYKSQLSCVVVDDIERLLDYVPIGPRFSNLVLQALLVLLKKAPPQGRKLLIIGTTSRKDVLQEMEMLDAF-STTIHIPNISSGEHLV 680          
BLAST of Vesicle-fusing ATPase vs. Ensembl Sea Lamprey
Match: nsfa (N-ethylmaleimide-sensitive factor a [Source:ZFIN;Acc:ZDB-GENE-030616-37])

HSP 1 Score: 354.369 bits (908), Expect = 1.674e-113
Identity = 196/543 (36.10%), Postives = 306/543 (56.35%), Query Frame = 2
Query:    8 AFKVQKNSYDEFSYTNKIFFNPSDWALFKEKFITVTTASNKFIFKSDKDESVKKGCMTTNGIQRKWMYCGIGEVVECIPYSF-QXXXXXXXXXXXXDLFVKSASLKDPIDSDKLAVKYLERFHDTPVTKNQLLLFNWESINFEAKIISIQGLD-------VKSSKMNEIETGIIQSNTVFKFQRRPESKFMLSGRCKAENAAVCLINPNWDFNSIGVGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKM---GNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEKFDPKKGIAIRIEDLQSFKINRQHFIFAIDHDVKALYGRHDDDLQEFKENRLLKWSPQISKALSTAGLLIESLKTNDNISRKRLLIYG 1603
            A K  +   DE S TN    + SD  L       +T    +++F      SV  G +  +  QRKW    +G+ V+  P+ F +T   +  + +E+D   K  +  +P DSDK+A  ++ +F     +  Q L+F++    F   +  I+ +D         S K N+I TG+   NT   F++   S   L+G+ K +     +INP+W+F  +G+GGLD EFS IF+ AF SR+FPP+ VE++ + HV+ + + G P T K++   ++G  +  R P   +G     + VG+ E   R+         ++    G  S LHIIIFDEIDAICK RG+++G + V DTVVNQLL+K+DGVEQLNNIL+IGMTNR D+IDEALLRPGR+ V++E+ LP   GR+QI  IHT ++R+  +LA D+D+ ELA  TKN+SGAE+E +V AA + A +        + + +E  +  ++ R+ F+ A+D D+K  +G + +D Q +  N ++ W   + + L    LL++  K ++      +L+ G
Sbjct:    5 AMKAARCPTDELSITNCAVVHESD--LKSIHVAVITGPGQRYVFTLKSHHSVVPGTIAFSLPQRKWAGLSLGQDVQVEPFEFDKTKNSIGTMTIEVDFLQKKTADSNPYDSDKMATDFVGQFISQSFSVGQQLVFSFAGKLFGLIVKGIEAVDPNILKGDATSGKQNKILTGVSYQNTQVIFEKAENSNLNLTGKSKTKQGRQSIINPDWNFEKMGIGGLDREFSDIFRRAFASRVFPPDIVEQMELSHVKCVPVEGKPPTFKSVMLNELGSTMQFRYPH--HGVERGREAVGDREWPSRQPPPTPPSRHRRCDQCGVNSGLHIIIFDEIDAICKQRGSMAGSTGVHDTVVNQLLSKIDGVEQLNNILVIGMTNRPDLIDEALLRPGRLEVKMEIGLPDNKGRIQILNIHTARMRDNGLLASDVDVNELAVETKNFSGAELEGLVRAAQSTAMNRHIKATTKVEVDMEKAERLQVTREDFLGALDSDIKPAFGTNTEDYQSYIMNGIVNWGEPVHRVLEDGDLLVQQAKMSERTPLVSVLLAG 543          
BLAST of Vesicle-fusing ATPase vs. Ensembl Sea Lamprey
Match: psmc5 (proteasome 26S subunit, ATPase 5 [Source:ZFIN;Acc:ZDB-GENE-030131-6547])

HSP 1 Score: 149.058 bits (375), Expect = 4.034e-39
Identity = 94/239 (39.33%), Postives = 136/239 (56.90%), Query Frame = 2
Query:  632 VGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKAR--GNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEK 1342
            +GGLD++  +I K      +  PE  E LGI   +G+LLYGPPGTGKTL AR +         + V+G  ++ K++GE    +R+LF         M  + A  II  DEID+I  +R  G   G S VQ T++ +LL ++DG E   NI +I  TNR D++D ALLRPGR+  +IE P P++  RL I +IH++K+     L   I+L+++A L    SGAE++ V   A   A  E+
Sbjct:  155 IGGLDKQIKEI-KEVIELPVKHPELFEALGIAQPKGVLLYGPPGTGKTLLARAVAHHTECTFIR-VSGSELVQKFIGEGSRMVRELFV--------MAREHAPSIIFMDEIDSIGSSRLEGGSGGDSEVQRTML-ELLNQLDGFEATKNIKVIMATNRIDILDPALLRPGRIDRKIEFPPPNEEARLDILKIHSRKMN----LTRGINLRKIAELMPGASGAEVKGVCTEAGMYALRER 378          
BLAST of Vesicle-fusing ATPase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000010299.1 (pep scaffold:Pmarinus_7.0:GL479893:343:6144:1 gene:ENSPMAG00000009320.1 transcript:ENSPMAT00000010299.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 138.272 bits (347), Expect = 2.247e-35
Identity = 83/231 (35.93%), Postives = 135/231 (58.44%), Query Frame = 2
Query:  632 VGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKAR--GNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAA 1318
            VGGLD++  ++ ++  L       F E LGI+  +G+L+YGPPGTGKTL AR       A   K+  GP ++  ++G+    +R  FA A+E+        A  II  DE+DAI   R     +G   VQ T++ +LL ++DG +    + +I  TNR D++D ALLR GR+  +IE P+P++  R +I +IH++K+     ++ D++ +ELA  T +++GA+ +AV + A
Sbjct:  164 VGGLDKQIQELVEAIVLPMTHKERF-ENLGIQPPKGVLMYGPPGTGKTLLARACAAQTKATFLKLA-GPQLVQMFIGDGAKLVRDAFALAKEK--------APTIIFIDELDAIGTKRFDSEKAGDREVQRTML-ELLNQLDGFQPNTQVKVIAATNRVDILDPALLRSGRLDRKIEFPMPNEEARARIMQIHSRKMN----VSPDVNFEELARCTDDFNGAQCKAVCVEA 379          
BLAST of Vesicle-fusing ATPase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001244.1 (pep scaffold:Pmarinus_7.0:GL482260:6416:9764:-1 gene:ENSPMAG00000001115.1 transcript:ENSPMAT00000001244.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 137.117 bits (344), Expect = 7.102e-35
Identity = 90/237 (37.97%), Postives = 131/237 (55.27%), Query Frame = 2
Query:  614 DFNSIGVGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKAR--GNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAA 1318
            D     VGG  E+  ++ +      L P  FV  LGI+  +G+LL+GPPGTGKTL AR +    +A   +++ G  ++ KYVGE    +R+LF        +M  ++ L  I FDEIDAI  AR      G + VQ T++ +L+ ++DG +   NI ++  TNR D +D AL+RPGR+  +IE  LP   GR  IF+IH + +     +  DI  + LA L  N +GAEI +V   A
Sbjct:  181 DVTYSDVGGCKEQIEKLREVVETPLLHPERFVN-LGIEPPKGVLLFGPPGTGKTLCARAVANRTDACFIRVI-GSELVQKYVGEGARMVRELF--------EMARKACL--IFFDEIDAIGGARFDDGAGGDNEVQRTML-ELINQLDGFDPRGNIKVLMATNRPDTLDPALMRPGRLDRKIEFGLPDLEGRTHIFKIHARSMS----VERDIRFELLARLCPNSTGAEIRSVCTEA 400          
BLAST of Vesicle-fusing ATPase vs. Ensembl Sea Lamprey
Match: nvl (nuclear VCP like [Source:ZFIN;Acc:ZDB-GENE-040426-2871])

HSP 1 Score: 135.576 bits (340), Expect = 9.547e-34
Identity = 93/247 (37.65%), Postives = 130/247 (52.63%), Query Frame = 2
Query:  608 NWDFNSIGVGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKI----VNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELA--ALTKNYSGAEIEAVVMAALANA 1330
             WD     +G L E   +    A L+ +  PE  + LG+    GILL GPPG GKT+ A+ I     A E  +    V GP +L+ YVGESE  +R++F  A          SA  +I FDEIDAIC  R +   G++V+  VVNQLL +MDG+E    + I+  TNR D++D A+LRPGR+   + V LP    R  I    T+    K  L  D+DL  +A    +  +SGA+++A+   A   A
Sbjct:  256 TWD----DIGAL-EAIREELSLAILAPIRHPEMFQVLGLTSPAGILLAGPPGCGKTMLAKAI-----ANESGLNFISVKGPELLNMYVGESERAVRQVFQRA--------RNSAPCVIFFDEIDAICPRRSDHESGASVR--VVNQLLTEMDGLEARKQVFIMAATNRPDILDRAVLRPGRLDKTLYVGLPVMAERAAILRTLTRG-GTKPPLEKDVDLNVIAGDGRSDGFSGADLQALTREAAIGA 481          

HSP 2 Score: 88.1965 bits (217), Expect = 2.143e-18
Identity = 58/168 (34.52%), Postives = 90/168 (53.57%), Query Frame = 2
Query:  827 VNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANA 1330
            V GP ++    GESE  +R+LF        ++ ++SA  ++  DEIDAI   R   S    ++  +V QLL  MDG+     +L IG TNR D +D AL R GR   +I + +P ++ R +I  +  + LR    L ++ + K LA LT  Y GA++ ++   A A+A
Sbjct:    9 VAGPEVVSGVSGESEQKLRELF--------QVASESAPCVLFLDEIDAITPKREMAS--KDMERRLVAQLLTCMDGLHGGGAVLCIGATNRPDALDPALRRAGRFDREICLGIPDENARERILLVLCRGLR----LKEEFNFKRLARLTPGYVGADLMSLCREAAASA 162          
BLAST of Vesicle-fusing ATPase vs. Ensembl Yeast
Match: SEC18 (AAA ATPase and SNARE disassembly chaperone; required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuole fusion, autophagy, and protein secretion; releases Sec17p from SNAP complexes; has similarity to mammalian N-ethylmaleimide-sensitive factor (NSF) [Source:SGD;Acc:S000000284])

HSP 1 Score: 490.345 bits (1261), Expect = 2.245e-163
Identity = 276/740 (37.30%), Postives = 437/740 (59.05%), Query Frame = 2
Query:   11 FKVQKNSYDEFSYTNKIFFNPSDWALFKEKFITVTTASNKFIFKSDKDESVKKGCMTTNGIQRKWMYCGIGEVVECIPYSFQXXXXXXXXXXXXDLFV----KSASLKDPIDSDKLAVKYLERFHDTPVTKNQLLLFNWESINFEAKIISIQGLDV-----KSSKMNEIET-GIIQSNTVFKFQRRPESKFMLSGRCKAENAAVCLINPNWDFNSIGVGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEKFDPKKGIA-IRIEDLQSFKINRQHFIFAIDHDVKALYGRHDDDLQEFKENRLLKWSPQISKALSTAGLLIESLKTNDNISRKRLLIYGEPLTGTSSVAVHLAKETGFTFIRMCSMRNMIGFSEPARCLAIKKIFDDALKAPASVIILDDVERLIDYSSIGPRFSIFLVNNLIGMINCFV----PKNKKMLIIGTTKHLSAMHELEFFSAFDKNCIKIPEIKTPSEIISVXXXXXXXXXXXXXXXXNHLQMGDCSDLAIGIKNLMENCKDLK-SVDPARRVDELLMK 2182
             KV     + ++  N    +P+D+      +I +    N F+F +     +  G +  NG QR W    + + V+   +           +  +D+ +    +  ++    D D+LA +++  +     +  Q L+  ++   F+ KI ++Q +D+      S+    IET GI+   T   F +  +    L         +  +I P++ F  +GVGGLD+EF++IF+ AF SR+FPP  +E+LGI HV+G+LLYGPPGTGKTL AR+IG MLNA+EPKIVNGP IL KYVG SE NIR LF DAE E++  G +S+LHIIIFDE+D++ K RG+   G+ V D VVNQLLAKMDGV+QLNNIL+IGMTNR+D+ID ALLRPGR  VQ+E+ LP + GRLQIF+I TKK+RE N+++DD++L ELAALTKN+SGAEIE +V +A + A ++  +  KG   +  +D+   K+ R+ F+ A+ +DV   +G  ++DL+   E  ++ +S +++  L      +  ++ +D      LLI+G   +G +++A  +A ++GF FIR+ S   + G SE A+   I   F DA K+P +++++D +E L+D+  IGPRFS    NN++ M+   +    P+++++LI+ TT   S + +++  S FD N I +P +    E+ +V+ + +   D+   ++ N L    C +  +GIK  + N +  +   DP   + EL+ +
Sbjct:   29 LKVSNCPNNSYALANVAAVSPNDFP--NNIYIII---DNLFVFTTRHSNDIPPGTIGFNGNQRTWGGWSLNQDVQAKAFDLFKYSGKQSYLGSIDIDISFRARGKAVSTVFDQDELAKQFVRCYESQIFSPTQYLIMEFQGHFFDLKIRNVQAIDLGDIEPTSAVATGIETKGILTKQTQINFFKGRDGLVNLKSSNSLRPRSNAVIRPDFKFEDLGVGGLDKEFTKIFRRAFASRIFPPSVIEKLGISHVKGLLLYGPPGTGKTLIARKIGTMLNAKEPKIVNGPEILSKYVGSSEENIRNLFKDAEAEYRAKGEESSLHIIIFDELDSVFKQRGSRGDGTGVGDNVVNQLLAKMDGVDQLNNILVIGMTNRKDLIDSALLRPGRFEVQVEIHLPDEKGRLQIFDIQTKKMRENNMMSDDVNLAELAALTKNFSGAEIEGLVKSASSFAINKTVNIGKGATKLNTKDIAKLKVTREDFLNAL-NDVTPAFGISEEDLKTCVEGGMMLYSERVNSILKNGARYVRQVRESDKSRLVSLLIHGPAGSGKTALAAEIALKSGFPFIRLISPNELSGMSESAKIAYIDNTFRDAYKSPLNILVIDSLETLVDWVPIGPRFS----NNILQMLKVALKRKPPQDRRLLIMTTTSAYSVLQQMDILSCFD-NEIAVPNMTNLDELNNVMIESNFLDDAGRVKVINELSRS-CPNFNVGIKKTLTNIETARHDEDPVNELVELMTQ 756          
BLAST of Vesicle-fusing ATPase vs. Ensembl Yeast
Match: CDC48 (AAA ATPase; subunit of polyUb-selective segregase complex involved in ERAD, INM-associated degradation (INMAD), mitotic spindle disassembly, macroautophagy, PMN, ribosome-associated degradation, ribophagy, homotypic ER membrane fusion, SCF complex disassembly, cell wall integrity during heat stress, and telomerase regulation; mobilizes membrane-anchored transcription factors by regulated Ub/proteasome-dependent processing (RUP); human ortholog VCP complements a cdc48 mutant [Source:SGD;Acc:S000002284])

HSP 1 Score: 176.792 bits (447), Expect = 1.448e-46
Identity = 151/458 (32.97%), Postives = 229/458 (50.00%), Query Frame = 2
Query:  614 DFNSIG---VGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEKFD--PKKGIAIRIEDLQSFKINRQHFIFAIDHD---------VKALYGRHDD--DLQEFKENRLLKWSPQISKALSTAGLLIESLKTNDNISRKRLLIYGEPLTGTSSVAVHLAKETGFTFI-----RMCSMRNMIGFSEPARCLAIKKIFDDALKAPASVIILDDVERLIDY--SSIGPR--FSIFLVNNLIGMINCFVPKNKKMLIIGTT 1912
            + N +G   +GG  ++ +QI +   L  L  P+  + +GIK  RG+L+YGPPGTGKTL AR +     A    ++NGP ++ K  GESE+N+RK F +AE        ++A  II  DEID+I   R   +G   V+  VV+QLL  MDG++  +N+++I  TNR + ID AL R GR   ++++ +P   GRL++  IHTK ++    LADD+DL+ LAA T  Y GA+I ++   A      EK D        I  E L S  +   +F FA+ +          V+++    DD   L E KE        ++ + +    L  +          K +L YG P TG + +A  +A E    FI      + SM    G SE      I+ IFD A  A  +V+ LD+++ +      S+G     S  +VN L+  ++    K K + +IG T
Sbjct:  207 NMNEVGYDDIGGCRKQMAQIREMVELP-LRHPQLFKAIGIKPPRGVLMYGPPGTGKTLMARAVANETGAFF-FLINGPEVMSKMAGESESNLRKAFEEAE--------KNAPAIIFIDEIDSIAPKRDKTNG--EVERRVVSQLLTLMDGMKARSNVVVIAATNRPNSIDPALRRFGRFDREVDIGIPDATGRLEVLRIHTKNMK----LADDVDLEALAAETHGYVGADIASLCSEAAMQQIREKMDLIDLDEDEIDAEVLDSLGVTMDNFRFALGNSNPSALRETVVESVNVTWDDVGGLDEIKE--------ELKETVEYPVLHPDQYTKFGLSPSKGVLFYGPPGTGKTLLAKAVATEVSANFISVKGPELLSM--WYGESES----NIRDIFDKARAAAPTVVFLDELDSIAKARGGSLGDAGGASDRVVNQLLTEMDGMNAK-KNVFVIGAT 633          

HSP 2 Score: 154.836 bits (390), Expect = 2.215e-39
Identity = 92/241 (38.17%), Postives = 134/241 (55.60%), Query Frame = 2
Query:  599 INPNWDFNSIGVGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARG-NLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAA 1318
            +N  WD     VGGLDE   ++ ++     L P ++ +  G+   +G+L YGPPGTGKTL A+ +   ++A     V GP +L  + GESE+NIR +F  A          +A  ++  DE+D+I KARG +L       D VVNQLL +MDG+    N+ +IG TNR D ID A+LRPGR+   I VPLP ++ RL I     +    K  L   ++L  +A  T+ +SGA++  +V  A
Sbjct:  482 VNVTWD----DVGGLDEIKEELKETVEYPVLHPDQYTK-FGLSPSKGVLFYGPPGTGKTLLAKAVATEVSANFIS-VKGPELLSMWYGESESNIRDIFDKAR--------AAAPTVVFLDELDSIAKARGGSLGDAGGASDRVVNQLLTEMDGMNAKKNVFVIGATNRPDQIDPAILRPGRLDQLIYVPLPDENARLSILNAQLR----KTPLEPGLELTAIAKATQGFSGADLLYIVQRA 704          
BLAST of Vesicle-fusing ATPase vs. Ensembl Yeast
Match: AFG2 (ATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexameric complex; is essential for pre-60S maturation and release of several preribosome maturation factors; releases Rlp24p from purified pre-60S particles in vitro; target of the ribosomal biosynthesis inhibitor diazaborine; may be involved in degradation of aberrant mRNAs [Source:SGD;Acc:S000004389])

HSP 1 Score: 152.525 bits (384), Expect = 1.040e-38
Identity = 122/402 (30.35%), Postives = 191/402 (47.51%), Query Frame = 2
Query:  629 GVGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAV----VMAALAN--AQDEKFDPKKGIAIRIEDLQSFKIN-RQHFIFAIDHDVKALYGRHDDDLQEFKE--NRLLKWSPQISKALSTAGLLIESLKTNDNISRKRLLIYGEPLTGTSSVAVHLAKETGFTFIRMCS---MRNMIGFSEPARCLAIKKIFDDALKAPASVIILDDVERL 1798
             VGGLD+E   + KSA    L  P      G+   RGILL+GPPGTGKT+  R +    NA    I NGPSI+ KY+GE+EA +R +F +A         +    II  DEID+I   R N   G  V+  VV  LL  MDG+     +++I  TNR + +D AL RPGR   ++E+ +P    R  I      ++     + D   +K +A+ T  Y GA++ A+    VM  +      D   D K  + + ++D++S  ++ R   +  I  ++  +Y       +E K     +++   + S+  +  G+           + K +L+YG P    +  A  LA E+G  F+ +         +G SE     AI++IF  A  A  S+I  D+++ L
Sbjct:  246 AVGGLDKEIESL-KSAIEIPLHQPTLFSSFGVSPPRGILLHGPPGTGKTMLLRVVANTSNAHVLTI-NGPSIVSKYLGETEAALRDIFNEA--------RKYQPSIIFIDEIDSIAPNRANDDSGE-VESRVVATLLTLMDGMGAAGKVVVIAATNRPNSVDPALRRPGRFDQEVEIGIPDVDARFDILTKQFSRMSSDRHVLDSEAIKYIASKTHGYVGADLTALCRESVMKTIQRGLGTDANID-KFSLKVTLKDVESAMVDIRPSAMREIFLEMPKVYWSDIGGQEELKTKMKEMIQLPLEASETFARLGI----------SAPKGVLLYGPPGCSKTLTAKALATESGINFLAVKGPEIFNKYVGESE----RAIREIFRKARSAAPSIIFFDEIDAL 621          

HSP 2 Score: 147.517 bits (371), Expect = 3.545e-37
Identity = 105/265 (39.62%), Postives = 136/265 (51.32%), Query Frame = 2
Query:  632 VGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKI----VNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEKFDPKKGIAIRIEDLQSFKINRQHF 1414
            +GG  EE     K      L   E    LGI   +G+LLYGPPG  KTLTA+ +     A E  I    V GP I +KYVGESE  IR++F  A          +A  II FDEIDA+   R   S  +A  + V+  LL ++DGVE+L  ++I+  TNR D ID ALLRPGR+   I V  P  + RL+I +  TKK    N     +DL ELA  T+ YSGAE+  +   A             G+A  +EDL   K+  +HF
Sbjct:  518 IGG-QEELKTKMKEMIQLPLEASETFARLGISAPKGVLLYGPPGCSKTLTAKAL-----ATESGINFLAVKGPEIFNKYVGESERAIREIFRKA--------RSAAPSIIFFDEIDALSPDRDGSSTSAA--NHVLTSLLNEIDGVEELKGVVIVAATNRPDEIDAALLRPGRLDRHIYVGPPDVNARLEILKKCTKKF---NTEESGVDLHELADRTEGYSGAEVVLLCQEA-------------GLAAIMEDLDVAKVELRHF 750          
BLAST of Vesicle-fusing ATPase vs. Ensembl Yeast
Match: RPT6 (ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress [Source:SGD;Acc:S000003016])

HSP 1 Score: 145.206 bits (365), Expect = 4.479e-38
Identity = 94/239 (39.33%), Postives = 134/239 (56.07%), Query Frame = 2
Query:  632 VGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGG--SAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEK 1342
            VGGL ++  +I K      +  PE  E LGI   +G++LYGPPGTGKTL AR +    + +  + V+G  ++ KY+GE    +R+LF         M  + A  II  DEID+I   R   SGG  S VQ T++ +LL ++DG E   NI II  TNR D++D ALLRPGR+  +IE P PS   R +I  IH++K+     L   I+L+++A      SGA+++ V   A   A  E+
Sbjct:  150 VGGLTKQIKEI-KEVIELPVKHPELFESLGIAQPKGVILYGPPGTGKTLLARAVAHHTDCKFIR-VSGAELVQKYIGEGSRMVRELFV--------MAREHAPSIIFMDEIDSIGSTRVEGSGGGDSEVQRTML-ELLNQLDGFETSKNIKIIMATNRLDILDPALLRPGRIDRKIEFPPPSVAARAEILRIHSRKMN----LTRGINLRKVAEKMNGCSGADVKGVCTEAGMYALRER 373          
BLAST of Vesicle-fusing ATPase vs. Ensembl Yeast
Match: PEX6 (AAA-peroxin; heterodimerizes with AAA-peroxin Pex1p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol; mutations in human PEX6 can lead to severe peroxisomal disorders and early death [Source:SGD;Acc:S000005273])

HSP 1 Score: 150.984 bits (380), Expect = 5.969e-38
Identity = 95/246 (38.62%), Postives = 136/246 (55.28%), Query Frame = 2
Query:  602 NPNWDFNSIGVGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVE-QLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLP-SQHGRLQIFEIHTKKLREKNILADDIDLKELAALTK-NYSGAEIEAVVMAALANA 1330
            N  WD     +GG+D    +I  +  +    P  F    G+K   GIL YGPPGTGKTL A+ I    +      V GP +L+ Y+GESEAN+R++F  A E        +   +I FDEID++   RGN      V D +V+QLLA++DG+    + + +IG TNR D++DEALLRPGR    + + +P +   +L I E  T+K     +L +D+ L ELA L   NY+GA+  A+   A+ NA
Sbjct:  728 NVTWD----DIGGIDFVKGEILDTIDMPLKHPELFTS--GMKKRSGILFYGPPGTGKTLMAKAIATNFSLNFFS-VKGPELLNMYIGESEANVRRVFQKARE--------AKPCVIFFDEIDSVAPKRGNQGDSGGVMDRIVSQLLAELDGMSTDADGVFVIGATNRPDLLDEALLRPGRFDKLLYLGIPDTDTKQLNILEALTRKF----VLDNDVKLIELAKLCPFNYTGADFYALCSDAMLNA 954          
BLAST of Vesicle-fusing ATPase vs. Ensembl Nematostella
Match: EDO36224 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SJ74])

HSP 1 Score: 544.273 bits (1401), Expect = 0.000e+0
Identity = 286/678 (42.18%), Postives = 425/678 (62.68%), Query Frame = 2
Query:    2 MIAFKVQKNSYDEFSYTNKIFFNPSDWALFKEKFITVTTASNK-FIFKSDKDESVKKGCMTTNGIQRKWMYCGIGEVVECIPYSFQXXXXXXXXXXXXDLFVKSASLKDPIDSDKLAVKYLERFHDTPVTKNQLLLFNWESINFEAKII-SIQGLDV------KSSKMNEIETGIIQSNTVFKFQRRPESKFMLSGRCKAENAAVCLINPNWDFNSIGVGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEKFDPKKGIAIRIEDLQSFKINRQHFIFAIDHDVKALYGRHDDDLQEFKENRLLKWSPQISKALSTAGLLIESLKTNDNISRKRLLIYGEPLTGTSSVAVHLAKETGFTFIRMCSMRNMIGFSEPARCLAIKKIFDDALKAPASVIILDDVERLIDYSSIGPRFSIFLVNNLIGMINCFVPKNKKMLIIGTTKHLSAMHELEFFSAFDKNCIKIPEIKTPSEIIS 2011
            M+  KV K   D+ S +N +  N +D    K K + V T   K FIF +   + + +G    + IQRKW    +   ++ IPY F     ++ + +E D   KS   +D  D+DK+A  + + F D   +  Q + F +        +I S++ +DV        S    +  G++  NT   F+R   S   L+G  K +     +I+ +WDF  +G+GGLD+EFS I + AF +RLFP + V+++G+KHV+GILL+GPPGTGKTL ARQIG MLN REPKI++GP +L+K+VGESEANIRKLFA+AEEE K+ G+ SALH+IIFDE DA+CK+R + +  + VQD+VVNQLLAK+DGVEQLNN+L+IGMTNRRD+ID+ALLRPGR+ VQ+E+ LP + GR+QI +IHT K+RE  +LADD+DL ELA  TKN++GAEIE +V AA + A +          I  +  Q   ++R+ F+ A++ D+K  +G  DD+L  +  N + +W   + + L    LL++  +  D  S   +L+ G    G +++A+ +A  + F FI++C+  NMIGF + A+C +IKKIFDDA K+  S II+DD+ERL+DY +IGPRFS  ++  L+ ++   + K +K++IIGTT     +  +    AF+   I +P + T   +++
Sbjct:    1 MVQLKVAKCPSDKLSLSNCLIVNENDLNAEKVKHVEVRTGGPKGFIFTTMVSDDIPQGSAGFSLIQRKWTSVSLNSYIDVIPYRFDESAYLSSITIEADFLQKSRPTQDAYDTDKMAAYFTDIFFDHAFSLGQQVPFGFHDKKLLLLVIKSMEVIDVGLLRAASESSSTNVSVGVLTRNTQVVFERAEGSTLTLTGGSKGKAIHQSIISRDWDFTKLGIGGLDKEFSNIVRRAFATRLFPADVVDKMGLKHVKGILLFGPPGTGKTLMARQIGTMLNTREPKIISGPEVLNKFVGESEANIRKLFAEAEEEQKRFGDNSALHLIIFDEFDAVCKSRYSYNVRTGVQDSVVNQLLAKIDGVEQLNNVLLIGMTNRRDLIDDALLRPGRLEVQVEIGLPDEEGRVQILKIHTAKMRENKVLADDVDLAELATQTKNFTGAEIEGLVRAAQSTAMNRFIKLTNNFEIDPDAAQKILVHREDFVQALECDIKPAFGPSDDNLDLYVANGIFRWGNHVQRILDDGRLLLQQTRVQDITSPITVLLEGPVGAGKTALAIQMALNSDFPFIKLCTPENMIGFVDSAKCQSIKKIFDDADKSDLSCIIVDDIERLLDYVAIGPRFSNTVLQALLVLLKKKLRKGRKLIIIGTTSCRDILEPMGMIEAFN-TVIHVPNLSTKDHLMN 677          
BLAST of Vesicle-fusing ATPase vs. Ensembl Nematostella
Match: EDO39006 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SBC8])

HSP 1 Score: 162.54 bits (410), Expect = 2.162e-42
Identity = 108/266 (40.60%), Postives = 144/266 (54.14%), Query Frame = 2
Query:  632 VGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKI----VNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVM-AALANAQDEKFDPKKGIAIRIEDLQSFKINRQHF 1414
            VGG +E   +  K A    L  PE  + LGI+  RGIL+YGPPG  KTL AR +     A E  +    + GP +  K+VGESE  +R++F  A          +A  I+ FDE+DAI   R N +GGS V D V+ QLL ++DGVE L +++ I  TNR DMID+AL+RPGR+   I VPLP    R  I EIH      +      +DL++L   T+ YSGAEI AV   AALA  Q              E++Q+  +  +HF
Sbjct:  310 VGG-NEMIKRKLKEAVEWPLKHPEAFQRLGIRPPRGILMYGPPGCSKTLIARAL-----ATESGLNFIAIKGPELFSKWVGESEKAVREVFLKA--------RATAPSIVFFDELDAIAGQR-NSTGGSDVNDRVLTQLLTELDGVETLKDVIFIAATNRPDMIDKALMRPGRVDRLIYVPLPCWDTRRHILEIHLA----RTPCEGSLDLEDLVERTEGYSGAEIAAVCREAALAALQ--------------ENIQAESVELRHF 542          

HSP 2 Score: 123.25 bits (308), Expect = 2.346e-29
Identity = 114/416 (27.40%), Postives = 193/416 (46.39%), Query Frame = 2
Query:  632 VGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKI----VNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNN---ILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAA--------LANAQD------EKFDP--KKGIAIRIEDLQ-SFKINRQHFIFAIDHDVKALYGRH--DDDLQEFKENRLLKWSPQISKALSTAGLLIESLKTNDNISRKRLLIYGEPLTGTSSVAVHLAKETGFTFIRMCS---MRNMIGFSEPARCLAIKKIFDDALKAPASVIILDDVE 1792
            +GGL  +  Q  +      L  PE     G+   RGILLYGP GTGKT+ AR +     A E  +    +NGP +L +Y GE+EA +R++F +A+       N+S   I+  DE+DA+C  R  +   +  +  VV  LL  MDG+   +    ++++  TNR D +D AL RPGR   +IE+ +PS   R  I     K +   + L D+ D+  LA     Y GA++ A    A        L N  +      E+ D   K+ + +  ED++ +F+  R   +  +  +V  ++      +++ + K    ++W  +  +A    G+             + +L+YG P    + +A  LA E+G  FI +         +G SE     A++++F  A     S++  D+++
Sbjct:   29 IGGLKTQI-QAVREMIEMPLTNPELFTAYGVPPPRGILLYGPSGTGKTMIARAV-----ANETGVHFFCINGPEVLSRYYGETEARLREIFTEAQ-------NKSP-SIVFIDELDALCPRRDKVQ--NEFERRVVATLLTLMDGMHMKSTDTYVMVLAATNRPDALDPALRRPGRFDREIEIGIPSVTDRRDILVTLLKNV--PHSLHDE-DISSLAESAHGYVGADLAAACKEASLYAFKRSLHNHDNGSSQSLEQRDARIKRELLVSSEDMRAAFRCVRPSAMREVALEVPKVHWSDVGGNEMIKRKLKEAVEWPLKHPEAFQRLGIR----------PPRGILMYGPPGCSKTLIARALATESGLNFIAIKGPELFSKWVGESEK----AVREVFLKARATAPSIVFFDELD 411          
BLAST of Vesicle-fusing ATPase vs. Ensembl Nematostella
Match: EDO36218 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SJ61])

HSP 1 Score: 160.999 bits (406), Expect = 6.376e-41
Identity = 128/421 (30.40%), Postives = 205/421 (48.69%), Query Frame = 2
Query:  617 FNSIG---VGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEKFD--PKKGIAIRIEDLQSFKINRQHFIFAID-------------------HDVKALYGRHDDDLQEFKENRLLKWSPQISKALSTAGLLIESLKTNDNISRKRLLIYGEPLTGTSSVAVHLAKETGFTFIRMCS---MRNMIGFSEPARCLAIKKIFDDALKAPASVIILDDVERL 1798
             N +G   +GG  ++ +QI +   L  L  P+  + +G+K  RGILL+GPPGTGKTL AR +     A    ++NGP I+ K  GESE+N+RK F +AE        +++  II  DEIDAI   R    G   V+  +V+QLL  MDG++Q ++++++  TNR + +D AL R GR   ++++ +P   GRL+I  IHTK ++    L DD+DL+++AA T  Y G+++ ++   A      EK D    +   I  E L S  ++   F +A+                     D+  L G    +LQE     L+++  +        G+             K +L YG P  G + +A  +A E    FI +     +    G SE      ++ +FD A  A   V+  D+++ +
Sbjct:  193 LNEVGYDDIGGCRKQLAQIKEMVELP-LRHPQLFKAIGVKPPRGILLFGPPGTGKTLMARAVANETGAFF-FLINGPEIMSKLAGESESNLRKAFEEAE--------KNSPAIIFIDEIDAIAPKRDKTHG--EVERRIVSQLLTLMDGLKQRSHVIVMAATNRPNSVDVALRRFGRFDREVDIGIPDATGRLEILRIHTKNMK----LGDDVDLEQIAAETHGYVGSDVASLCSEAALQQIREKMDLIDLEDETIDAEVLDSLAVSMDDFRYAMGVSNPSALRETVVEVPNVSWDDIGGLEGVK-RELQE-----LVQYPVEHPDKFLKFGM----------TPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESE----ANVRDVFDKARSAAPCVLFFDELDSI 577          

HSP 2 Score: 135.576 bits (340), Expect = 7.309e-33
Identity = 87/240 (36.25%), Postives = 132/240 (55.00%), Query Frame = 2
Query:  605 PNWDFNSIG-VGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSA-VQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAA 1318
            PN  ++ IG + G+  E  ++ +        P +F++  G+   +G+L YGPPG GKTL A+ I     A    I  GP +L  + GESEAN+R +F  A          +A  ++ FDE+D+I K+RG   G      D V+NQ+L +MDG+    N+ IIG TNR D+ID A+LRPGR+   I +PLP    R  I + + +    K+ +A D+DL  +A +T  +SGA++  +   A
Sbjct:  467 PNVSWDDIGGLEGVKRELQELVQYPVE---HPDKFLK-FGMTPSKGVLFYGPPGCGKTLLAKAIANECQANFISI-KGPELLTMWFGESEANVRDVFDKA--------RSAAPCVLFFDELDSIAKSRGGNVGDGGGAADRVINQVLTEMDGMNVKKNVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDDGSRSSILKANLR----KSPIAKDVDLDYVAKVTHGFSGADLTEICQRA 689          
BLAST of Vesicle-fusing ATPase vs. Ensembl Nematostella
Match: EDO49524 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RGH4])

HSP 1 Score: 149.058 bits (375), Expect = 5.162e-39
Identity = 96/239 (40.17%), Postives = 135/239 (56.49%), Query Frame = 2
Query:  632 VGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKAR--GNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEK 1342
            VGGLD++  +I K      +  PE  E LGI   +G+LLYGPPGTGKTL AR +         + V+G  ++ K++GE    +R+LF         M  + A  II  DEID+I  +R  G   G S VQ T++ +LL ++DG E   NI +I  TNR D++D ALLRPGR+  +IE P P++  R  I +IH++K+     L   I+LK++A L    SGAEI+ V   A   A  E+
Sbjct:  143 VGGLDKQIKEI-KEVIELPVKHPELFEALGIDQPKGVLLYGPPGTGKTLLARAVAHHTECTFIR-VSGSELVQKFIGEGARMVRELFV--------MAREHAPSIIFMDEIDSIGSSRLEGGSGGDSEVQRTML-ELLNQLDGFEATKNIKVIMATNRIDILDSALLRPGRIDRKIEFPPPNEEARQDILKIHSRKMN----LTRGINLKKIAELMPGASGAEIKGVCTEAGMYALRER 366          
BLAST of Vesicle-fusing ATPase vs. Ensembl Nematostella
Match: EDO37473 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SFJ3])

HSP 1 Score: 137.117 bits (344), Expect = 1.351e-34
Identity = 87/237 (36.71%), Postives = 129/237 (54.43%), Query Frame = 2
Query:  614 DFNSIGVGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKAR--GNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAA 1318
            D     +GG  E+  ++ +      L P  FV  LGI+  +G+LL+GPPGTGKTL AR +    +A   +++ G  ++ KYVGE    +R+LF        +M       I+ FDEIDAI  AR      G + VQ T++ +L+ ++DG +   NI ++  TNR D +D AL+RPGR+  ++E  LP   GR  IF+IH + +     +  DI  + LA L  N +GAEI +V   A
Sbjct:  172 DVTYSDIGGCKEQIDKLREVVETPLLHPERFVN-LGIEPPKGVLLFGPPGTGKTLCARAVANRTDACFIRVI-GSELVQKYVGEGARMVRELF--------EMARTKKACIVFFDEIDAIGGARFDDGAGGDNEVQRTML-ELINQLDGFDPRGNIKVLMATNRPDTLDPALMRPGRLDRKVEFGLPDLEGRAHIFKIHARSMS----VERDIRYELLARLCPNSTGAEIRSVCTEA 393          
BLAST of Vesicle-fusing ATPase vs. Ensembl Medaka
Match: NSF (vesicle-fusing ATPase [Source:NCBI gene;Acc:101155094])

HSP 1 Score: 572.392 bits (1474), Expect = 0.000e+0
Identity = 292/668 (43.71%), Postives = 428/668 (64.07%), Query Frame = 2
Query:   35 DEFSYTNKIFFNPSDWALFKEKFITV-TTASNKFIFKSDKDESVKKGCMTTNGIQRKWMYCGIGEVVECIPYSFQXXXXXXXXXXXXDLFVKSASL-KDPIDSDKLAVKYLERFHDTPVTKNQLLLFNWESINFEAKIISIQGLD--------VKSSKMNEIETGIIQSNTVFKFQRRPESKFMLSGRCKAENAAVCLINPNWDFNSIGVGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEKFDPKKGIAIRIEDLQSFKINRQHFIFAIDHDVKALYGRHDDDLQEFKENRLLKWSPQISKALSTAGLLIESLKTNDNISRKRLLIYGEPLTGTSSVAVHLAKETGFTFIRMCSMRNMIGFSEPARCLAIKKIFDDALKAPASVIILDDVERLIDYSSIGPRFSIFLVNNLIGMINCFVPKNKKMLIIGTTKHLSAMHELEFFSAFDKNCIKIPEIKTPSEII 2008
            DE S TN    +  D  L   + +TV TT ++K++F      +V  G +  +  QRKW    IG+ +E   Y+F         +     F++  S+   P DSDK+A ++L++F++   +  Q L+F++    F   +  I+ +D            K  +I+ G++  N+   F++   S   L G+ K + A   +INP+W+F  +G+GGLD+EFS IF+ AF SR+FPP+ VE++G KHV+GILL+GPPG GKTL ARQIGKMLNAREPK+VNGP IL+KYVGESEANIRKLFADAEEE K++G  S LHIIIFDE+DAICK RG  +  + V DTVVNQLL+K+DGVEQLNNIL+IGMTNR D+ID+AL+RPGR  V++E+ LP + GR+QI  IHT K+R  N+LA D+D+KELAA TKNYSGAE+E +V AA + A +        + + +E  +  ++ R  F+ ++++D+K  +G + +D   +  N ++KW   ++  L    LL++  K +D      +L+ G P +G +++A  +A+++ F FI++CS   MIG SE ++C AIKK+FDDA K+  S +++DD+ERL+DY  IGPRFS  ++  L+ ++    PK +K+LIIGTT     + E+E   AF    I +P I T  +++
Sbjct:   14 DELSLTNCAVVSEKD--LQSGQHVTVKTTPNHKYVFTVKTHHTVAPGTIAFSLPQRKWAGLSIGQDIEVSNYNFDKSNQCIGAMTIEIDFLQKKSMDSSPYDSDKMAAEFLQQFNNQAFSVTQQLVFSFCDKLFGLMVKDIEAMDSSILKGEPATGKKQQKIDVGLMVGNSQVIFEKAETSSLTLVGKAKTKEARQTIINPDWNFEKMGIGGLDKEFSDIFRRAFASRVFPPDIVEQMGCKHVKGILLFGPPGCGKTLMARQIGKMLNAREPKVVNGPEILNKYVGESEANIRKLFADAEEEQKRLGANSGLHIIIFDELDAICKQRGTGASSTGVHDTVVNQLLSKIDGVEQLNNILVIGMTNRPDLIDDALMRPGRFEVKMEIGLPDEKGRVQILNIHTIKMRNFNLLAADVDIKELAAETKNYSGAELEGLVRAAQSTAMNRHIKATSTVEVDMERAEKLQVTRDDFMGSLNNDIKPAFGTNQEDYSSYVMNGIIKWGDPVAHVLDDGELLVQQTKNSDRTPLVAVLLEGPPHSGKTALAAKIAEDSQFPFIKICSPDKMIGSSEISKCQAIKKVFDDAYKSQLSCVVVDDIERLLDYVPIGPRFSNLVLQALLVLLKKTPPKGRKLLIIGTTSRKDVLQEMEMLDAF-STTIHVPNISTGEQLV 678          
BLAST of Vesicle-fusing ATPase vs. Ensembl Medaka
Match: NSF (vesicle-fusing ATPase [Source:NCBI gene;Acc:101155094])

HSP 1 Score: 571.622 bits (1472), Expect = 0.000e+0
Identity = 292/668 (43.71%), Postives = 428/668 (64.07%), Query Frame = 2
Query:   35 DEFSYTNKIFFNPSDWALFKEKFITV-TTASNKFIFKSDKDESVKKGCMTTNGIQRKWMYCGIGEVVECIPYSFQXXXXXXXXXXXXDLFVKSASL-KDPIDSDKLAVKYLERFHDTPVTKNQLLLFNWESINFEAKIISIQGLD--------VKSSKMNEIETGIIQSNTVFKFQRRPESKFMLSGRCKAENAAVCLINPNWDFNSIGVGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEKFDPKKGIAIRIEDLQSFKINRQHFIFAIDHDVKALYGRHDDDLQEFKENRLLKWSPQISKALSTAGLLIESLKTNDNISRKRLLIYGEPLTGTSSVAVHLAKETGFTFIRMCSMRNMIGFSEPARCLAIKKIFDDALKAPASVIILDDVERLIDYSSIGPRFSIFLVNNLIGMINCFVPKNKKMLIIGTTKHLSAMHELEFFSAFDKNCIKIPEIKTPSEII 2008
            DE S TN    +  D  L   + +TV TT ++K++F      +V  G +  +  QRKW    IG+ +E   Y+F         +     F++  S+   P DSDK+A ++L++F++   +  Q L+F++    F   +  I+ +D            K  +I+ G++  N+   F++   S   L G+ K + A   +INP+W+F  +G+GGLD+EFS IF+ AF SR+FPP+ VE++G KHV+GILL+GPPG GKTL ARQIGKMLNAREPK+VNGP IL+KYVGESEANIRKLFADAEEE K++G  S LHIIIFDE+DAICK RG  +  + V DTVVNQLL+K+DGVEQLNNIL+IGMTNR D+ID+AL+RPGR  V++E+ LP + GR+QI  IHT K+R  N+LA D+D+KELAA TKNYSGAE+E +V AA + A +        + + +E  +  ++ R  F+ ++++D+K  +G + +D   +  N ++KW   ++  L    LL++  K +D      +L+ G P +G +++A  +A+++ F FI++CS   MIG SE ++C AIKK+FDDA K+  S +++DD+ERL+DY  IGPRFS  ++  L+ ++    PK +K+LIIGTT     + E+E   AF    I +P I T  +++
Sbjct:   20 DELSLTNCAVVSEKD--LQSGQHVTVKTTPNHKYVFTVKTHHTVAPGTIAFSLPQRKWAGLSIGQDIEVSNYNFDKSNQCIGAMTIEIDFLQKKSMDSSPYDSDKMAAEFLQQFNNQAFSVTQQLVFSFCDKLFGLMVKDIEAMDSSILKGEPATGKKQQKIDVGLMVGNSQVIFEKAETSSLTLVGKAKTKEARQTIINPDWNFEKMGIGGLDKEFSDIFRRAFASRVFPPDIVEQMGCKHVKGILLFGPPGCGKTLMARQIGKMLNAREPKVVNGPEILNKYVGESEANIRKLFADAEEEQKRLGANSGLHIIIFDELDAICKQRGTGASSTGVHDTVVNQLLSKIDGVEQLNNILVIGMTNRPDLIDDALMRPGRFEVKMEIGLPDEKGRVQILNIHTIKMRNFNLLAADVDIKELAAETKNYSGAELEGLVRAAQSTAMNRHIKATSTVEVDMERAEKLQVTRDDFMGSLNNDIKPAFGTNQEDYSSYVMNGIIKWGDPVAHVLDDGELLVQQTKNSDRTPLVAVLLEGPPHSGKTALAAKIAEDSQFPFIKICSPDKMIGSSEISKCQAIKKVFDDAYKSQLSCVVVDDIERLLDYVPIGPRFSNLVLQALLVLLKKTPPKGRKLLIIGTTSRKDVLQEMEMLDAF-STTIHVPNISTGEQLV 684          
BLAST of Vesicle-fusing ATPase vs. Ensembl Medaka
Match: NSF (vesicle-fusing ATPase [Source:NCBI gene;Acc:101155094])

HSP 1 Score: 571.622 bits (1472), Expect = 0.000e+0
Identity = 292/668 (43.71%), Postives = 428/668 (64.07%), Query Frame = 2
Query:   35 DEFSYTNKIFFNPSDWALFKEKFITV-TTASNKFIFKSDKDESVKKGCMTTNGIQRKWMYCGIGEVVECIPYSFQXXXXXXXXXXXXDLFVKSASL-KDPIDSDKLAVKYLERFHDTPVTKNQLLLFNWESINFEAKIISIQGLD--------VKSSKMNEIETGIIQSNTVFKFQRRPESKFMLSGRCKAENAAVCLINPNWDFNSIGVGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEKFDPKKGIAIRIEDLQSFKINRQHFIFAIDHDVKALYGRHDDDLQEFKENRLLKWSPQISKALSTAGLLIESLKTNDNISRKRLLIYGEPLTGTSSVAVHLAKETGFTFIRMCSMRNMIGFSEPARCLAIKKIFDDALKAPASVIILDDVERLIDYSSIGPRFSIFLVNNLIGMINCFVPKNKKMLIIGTTKHLSAMHELEFFSAFDKNCIKIPEIKTPSEII 2008
            DE S TN    +  D  L   + +TV TT ++K++F      +V  G +  +  QRKW    IG+ +E   Y+F         +     F++  S+   P DSDK+A ++L++F++   +  Q L+F++    F   +  I+ +D            K  +I+ G++  N+   F++   S   L G+ K + A   +INP+W+F  +G+GGLD+EFS IF+ AF SR+FPP+ VE++G KHV+GILL+GPPG GKTL ARQIGKMLNAREPK+VNGP IL+KYVGESEANIRKLFADAEEE K++G  S LHIIIFDE+DAICK RG  +  + V DTVVNQLL+K+DGVEQLNNIL+IGMTNR D+ID+AL+RPGR  V++E+ LP + GR+QI  IHT K+R  N+LA D+D+KELAA TKNYSGAE+E +V AA + A +        + + +E  +  ++ R  F+ ++++D+K  +G + +D   +  N ++KW   ++  L    LL++  K +D      +L+ G P +G +++A  +A+++ F FI++CS   MIG SE ++C AIKK+FDDA K+  S +++DD+ERL+DY  IGPRFS  ++  L+ ++    PK +K+LIIGTT     + E+E   AF    I +P I T  +++
Sbjct:   20 DELSLTNCAVVSEKD--LQSGQHVTVKTTPNHKYVFTVKTHHTVAPGTIAFSLPQRKWAGLSIGQDIEVSNYNFDKSNQCIGAMTIEIDFLQKKSMDSSPYDSDKMAAEFLQQFNNQAFSVTQQLVFSFCDKLFGLMVKDIEAMDSSILKGEPATGKKQQKIDVGLMVGNSQVIFEKAETSSLTLVGKAKTKEARQTIINPDWNFEKMGIGGLDKEFSDIFRRAFASRVFPPDIVEQMGCKHVKGILLFGPPGCGKTLMARQIGKMLNAREPKVVNGPEILNKYVGESEANIRKLFADAEEEQKRLGANSGLHIIIFDELDAICKQRGTGASSTGVHDTVVNQLLSKIDGVEQLNNILVIGMTNRPDLIDDALMRPGRFEVKMEIGLPDEKGRVQILNIHTIKMRNFNLLAADVDIKELAAETKNYSGAELEGLVRAAQSTAMNRHIKATSTVEVDMERAEKLQVTRDDFMGSLNNDIKPAFGTNQEDYSSYVMNGIIKWGDPVAHVLDDGELLVQQTKNSDRTPLVAVLLEGPPHSGKTALAAKIAEDSQFPFIKICSPDKMIGSSEISKCQAIKKVFDDAYKSQLSCVVVDDIERLLDYVPIGPRFSNLVLQALLVLLKKTPPKGRKLLIIGTTSRKDVLQEMEMLDAF-STTIHVPNISTGEQLV 684          
BLAST of Vesicle-fusing ATPase vs. Ensembl Medaka
Match: nsfb (vesicle-fusing ATPase [Source:NCBI gene;Acc:101174468])

HSP 1 Score: 567.385 bits (1461), Expect = 0.000e+0
Identity = 308/734 (41.96%), Postives = 452/734 (61.58%), Query Frame = 2
Query:    8 AFKVQKNSYDEFSYTNKIFFNPSDWALFKEKFITVTTASNKFIFKSDKDESVKKGCMTTNGIQRKWMYCGIGEVVECIPYSF-QXXXXXXXXXXXXDLFVKSASLKDPIDSDKLAVKYLERFHDTPVTKNQLLLFNWESINFEAKIISIQGLDV------KSSKMNEIETGIIQSNTVFKFQRRPESKFMLSGRCKAENAAVCLINPNWDFNSIGVGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEKFDPKKGIAIRIEDLQSFKINRQHFIFAIDHDVKALYGRHDDDLQEFKENRLLKWSPQISKALSTAGLLIESLKTNDNISRKRLLIYGEPLTGTSSVAVHLAKETGFTFIRMCSMRNMIGFSEPARCLAIKKIFDDALKAPASVIILDDVERLIDYSSIGPRFSIFLVNNLIGMINCFVPKNKKMLIIGTTKHLSAMHELEFFSAFDKNCIKIPEIKTPSEIISVXXXXXXXXXXXXXXXXNHLQMGDCSDLAIGIKNLMENCKDLKSVDPARRVDELLMKLK 2188
              K  K   DE S TN    N  +     E  +TV     +F+F   K   V  GC+  +  QRKW    +G+  +   Y F ++   ++ + +E+D   K      P DSDK+A + ++RF +   T  Q L+F++    F   +  I+ +D       + +  ++ + G++ +N+   F++   S  +L G+ K       +I+P+W+F  +G+GGLD+EFS IF+ AF SR+FP + +E++G KHV+GILLYGPPG GKTL ARQIGKML AREPKIVNGP IL+KYVGESEANIRKLFADAEEE K++G  S LHIIIFDEIDAICK RG+++G + V DTVVNQLL+K+DGVEQLNNIL+IGMTNR D+IDEALLRPGR+ V++E+ LP + GR+QI  IHT K+R+ N+LA D+D+KELA  TKNYSGAE+E +V AA + A +        + + I+  +   ++R  F+ A+++D+K  +G + +D   +  N +++W   +S  L    LL++  K +D      +L+ G   +G +++A  +++++ F FI++CS   MIGFSE A+C AIKKIF+DA K+  S +++DD+ERL+DY  IGPRFS  ++  L+ ++    PK +K+LIIGTT     + E+E   AF    + IP I    +++  L   D + + +   I   L+      L IGIK L+   +    +D   RV + L  LK
Sbjct:    5 TLKAVKCPTDELSLTNCAVINDKEQHF--EPHVTVYNGKGEFVFTLKKHPEVSSGCIAFSLPQRKWAAIVLGDDFKVTNYKFDKSKDCISVMTMEIDFLQKKFVDSSPFDSDKMASELVQRFDNQAFTVGQQLVFSFADKLFNLVVKEIEAMDASILKGERQAGGSKKKVGLLLANSQVIFEKSDNSAVVLIGKSKTRENRQSIISPDWNFERMGIGGLDKEFSDIFRRAFASRVFPSDIIEQMGCKHVKGILLYGPPGCGKTLMARQIGKMLRAREPKIVNGPEILNKYVGESEANIRKLFADAEEEQKRLGANSGLHIIIFDEIDAICKQRGSMAGSTGVHDTVVNQLLSKIDGVEQLNNILVIGMTNRPDLIDEALLRPGRLEVKMEIGLPDERGRIQILHIHTAKMRQYNLLASDVDVKELATETKNYSGAELEGLVRAAQSTAMNRHIKASSTVEVDIQTAEKLLVSRLDFMAALNNDIKPAFGTNQEDYASYIMNGIIRWGDPVSAVLEDGELLVQQTKNSDRTPLVSVLLEGPANSGKTALAAKISEDSEFPFIKICSPDKMIGFSETAKCQAIKKIFEDAYKSQLSCVVVDDIERLLDYVPIGPRFSNLVLQALLVLLKKPPPKGRKLLIIGTTSRKDVLQEMEMLDAFS-TTVHIPNISGGEQLVRALELLDSFQEKERSAIAKALK---GQRLWIGIKKLLMLIEMAAQMDADNRVRKFLSLLK 732          
BLAST of Vesicle-fusing ATPase vs. Ensembl Medaka
Match: nsfb (vesicle-fusing ATPase [Source:NCBI gene;Acc:101174468])

HSP 1 Score: 421.779 bits (1083), Expect = 2.469e-138
Identity = 222/492 (45.12%), Postives = 314/492 (63.82%), Query Frame = 2
Query:    2 MIAFKVQKNSYDEFSYTNKIFFNPSDWALFKEKFITVTTASNKFIFKSDKDESVKKGCMTTNGIQR-----KWMYCGIGEVVECIPYSF-QXXXXXXXXXXXXDLFVKSASLKDPIDSDKLAVKYLERFHDTPVTKNQLLLFNWESINFEAKIISIQGLDV------KSSKMNEIETGIIQSNTVFKFQRRPESKFMLSGRCKAENAAVCLINPNWDFNSIGVGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEKFDPKKGIAIRIEDLQSFKINRQHFIFAIDHDVK 1441
            +  +   K   DE S TN    N       KE+ +TV     +F+F   K   V  GC+  +  Q      +   C +        Y F ++   ++ + +E+D   K      P DSDK+A + ++RF +   T  Q L+F++    F   +  I+ +D       + +  ++ + G++ +N+   F++   S  +L G+ K       +I+P+W+F  +G+GGLD+EFS IF+ AF SR+FP + +E++G KHV+GILLYGPPG GKTL ARQIGKML AREPKIVNGP IL+KYVGESEANIRKLFADAEEE K++G  S LHIIIFDEIDAICK RG+++G + V DTVVNQLL+K+DGVEQLNNIL+IGMTNR D+IDEALLRPGR+ V++E+ LP + GR+QI  IHT K+R+ N+LA D+D+KELA  TKNYSGAE+E +V AA + A +        + + I+  +   ++R  F+ A+++D+K
Sbjct:    1 LAVYSAVKCPTDELSLTNCAVIND------KEQHVTVYNGKGEFVFTLKKHPEVSSGCIAFSLPQVAHPPVQPFICFVTN------YKFDKSKDCISVMTMEIDFLQKKFVDSSPFDSDKMASELVQRFDNQAFTVGQQLVFSFADKLFNLVVKEIEAMDASILKGERQAGGSKKKVGLLLANSQVIFEKSDNSAVVLIGKSKTRENRQSIISPDWNFERMGIGGLDKEFSDIFRRAFASRVFPSDIIEQMGCKHVKGILLYGPPGCGKTLMARQIGKMLRAREPKIVNGPEILNKYVGESEANIRKLFADAEEEQKRLGANSGLHIIIFDEIDAICKQRGSMAGSTGVHDTVVNQLLSKIDGVEQLNNILVIGMTNRPDLIDEALLRPGRLEVKMEIGLPDERGRIQILHIHTAKMRQYNLLASDVDVKELATETKNYSGAELEGLVRAAQSTAMNRHIKASSTVEVDIQTAEKLLVSRLDFMAALNNDIK 480          
BLAST of Vesicle-fusing ATPase vs. Planmine SMEST
Match: SMESG000019983.1 (SMESG000019983.1)

HSP 1 Score: 1447.18 bits (3745), Expect = 0.000e+0
Identity = 736/736 (100.00%), Postives = 736/736 (100.00%), Query Frame = 2
Query:    2 MIAFKVQKNSYDEFSYTNKIFFNPSDWALFKEKFITVTTASNKFIFKSDKDESVKKGCMTTNGIQRKWMYCGIGEVVECIPYSFQXXXXXXXXXXXXDLFVKSASLKDPIDSDKLAVKYLERFHDTPVTKNQLLLFNWESINFEAKIISIQGLDVKSSKMNEIETGIIQSNTVFKFQRRPESKFMLSGRCKAENAAVCLINPNWDFNSIGVGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEKFDPKKGIAIRIEDLQSFKINRQHFIFAIDHDVKALYGRHDDDLQEFKENRLLKWSPQISKALSTAGLLIESLKTNDNISRKRLLIYGEPLTGTSSVAVHLAKETGFTFIRMCSMRNMIGFSEPARCLAIKKIFDDALKAPASVIILDDVERLIDYSSIGPRFSIFLVNNLIGMINCFVPKNKKMLIIGTTKHLSAMHELEFFSAFDKNCIKIPEIKTPSEIISVXXXXXXXXXXXXXXXXNHLQMGDCSDLAIGIKNLMENCKDLKSVDPARRVDELLMKLKSSSEFQF 2209
            MIAFKVQKNSYDEFSYTNKIFFNPSDWALFKEKFITVTTASNKFIFKSDKDESVKKGCMTTNGIQRKWMYCGIGEVVECIPYSFQTVVLVTEVIVELDLFVKSASLKDPIDSDKLAVKYLERFHDTPVTKNQLLLFNWESINFEAKIISIQGLDVKSSKMNEIETGIIQSNTVFKFQRRPESKFMLSGRCKAENAAVCLINPNWDFNSIGVGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEKFDPKKGIAIRIEDLQSFKINRQHFIFAIDHDVKALYGRHDDDLQEFKENRLLKWSPQISKALSTAGLLIESLKTNDNISRKRLLIYGEPLTGTSSVAVHLAKETGFTFIRMCSMRNMIGFSEPARCLAIKKIFDDALKAPASVIILDDVERLIDYSSIGPRFSIFLVNNLIGMINCFVPKNKKMLIIGTTKHLSAMHELEFFSAFDKNCIKIPEIKTPSEIISVLADQDLYSDSDLQQIDNHLQMGDCSDLAIGIKNLMENCKDLKSVDPARRVDELLMKLKSSSEFQF
Sbjct:    1 MIAFKVQKNSYDEFSYTNKIFFNPSDWALFKEKFITVTTASNKFIFKSDKDESVKKGCMTTNGIQRKWMYCGIGEVVECIPYSFQTVVLVTEVIVELDLFVKSASLKDPIDSDKLAVKYLERFHDTPVTKNQLLLFNWESINFEAKIISIQGLDVKSSKMNEIETGIIQSNTVFKFQRRPESKFMLSGRCKAENAAVCLINPNWDFNSIGVGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEKFDPKKGIAIRIEDLQSFKINRQHFIFAIDHDVKALYGRHDDDLQEFKENRLLKWSPQISKALSTAGLLIESLKTNDNISRKRLLIYGEPLTGTSSVAVHLAKETGFTFIRMCSMRNMIGFSEPARCLAIKKIFDDALKAPASVIILDDVERLIDYSSIGPRFSIFLVNNLIGMINCFVPKNKKMLIIGTTKHLSAMHELEFFSAFDKNCIKIPEIKTPSEIISVLADQDLYSDSDLQQIDNHLQMGDCSDLAIGIKNLMENCKDLKSVDPARRVDELLMKLKSSSEFQF 736          
BLAST of Vesicle-fusing ATPase vs. Planmine SMEST
Match: SMESG000047180.1 (SMESG000047180.1)

HSP 1 Score: 157.147 bits (396), Expect = 1.453e-41
Identity = 100/239 (41.84%), Postives = 142/239 (59.41%), Query Frame = 2
Query:  608 NWDFNSIGVGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGN--LSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAA 1318
            N D+NSIG  GL+E+  ++ +   L  L  PE    +GIK  +G+LLYGPPGTGKTL AR +   L+A   K+V+  SI+DK++GES   IR++FA A+E            +I  DEIDAI   R N   S    +Q T++ +LL +MDG + L  + II  TNR D +D ALLRPGR+  +IE+PLP + GRL I +IH+ K+ +      +ID   L  L+  ++GA++  V   A
Sbjct:  130 NIDYNSIG--GLNEQIRELREVIELP-LKNPEIFVRVGIKAPKGVLLYGPPGTGKTLLARAVASQLDANFLKVVSS-SIVDKFIGESARVIREMFAYAKEH--------EPCVIFMDEIDAIGGRRFNNGTSSDREIQRTLM-ELLNQMDGFDVLGQVKIIMATNRPDTLDPALLRPGRLDRKIEIPLPKEQGRLDILKIHSDKISKHG----EIDWDSLVKLSDGFNGADMRNVCTEA 351          
BLAST of Vesicle-fusing ATPase vs. Planmine SMEST
Match: SMESG000009924.1 (SMESG000009924.1)

HSP 1 Score: 161.77 bits (408), Expect = 6.581e-41
Identity = 133/438 (30.37%), Postives = 210/438 (47.95%), Query Frame = 2
Query:  578 ENAAVCLINPNWDFNSIG---VGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPK----IVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEKFD--PKKGIAIRIEDLQSFKINRQHFIFAID-------------------HDVKALYGRHDDDLQEFKENRLLKWSPQISKALSTAGLLIESLKTNDNISRKRLLIYGEPLTGTSSVAVHLAKETGFTFIRMCS---MRNMIGFSEPARCLAIKKIFDDALKAPASVIILDDVERL 1798
            E   V   + +   N +G   +GG  ++ +QI +   L  L  P+  + +G+K  RGILLYGPPGTGKTL AR +     A E      ++NGP I+ K  GESE+N+RK F +AE        +++  II  DE+DAI   R    G   V+  +V+QLL  MDG++Q ++++++  TNR + ID AL R GR   ++++ +P   GRL+I  IHTK +R    L DD+DL+++A+ T  + GA++ A+   A       K D    +   I  E L S  +  + F +A+                     D+  L G    +LQE     L+++  +        G+             K +L YG P  G + +A  +A E    FI +     +    G SE      ++ IFD A +A   V+  D+++ +
Sbjct:  182 EGDPVVRADEDEKLNEVGYDDIGGCRKQLAQIKEMVELP-LRHPQLFKAIGVKPPRGILLYGPPGTGKTLIARAV-----ANETGSFFFLINGPEIMSKLAGESESNLRKAFEEAE--------KNSPAIIFIDELDAIAPKREKTHG--EVERRIVSQLLTLMDGLKQRSHVIVMAATNRPNSIDPALRRFGRFDREVDIGIPDTTGRLEILRIHTKNMR----LGDDVDLEQVASETHGHVGADLAALCTEAALQQIRNKMDLIDLEEPEIDAEILNSLAVTMEDFRWALSKSNPSALRETTVEVPNVTWEDIGGLQGVK-RELQE-----LVQYPVEHPDKFLKFGM----------TPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESE----ANVRDIFDKARQAAPCVLFFDELDSI 579          

HSP 2 Score: 144.436 bits (363), Expect = 2.630e-35
Identity = 102/308 (33.12%), Postives = 166/308 (53.90%), Query Frame = 2
Query:  605 PNWDFNSIG-VGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSA-VQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAE------------IEAVVMAALANAQDEKFDPKKGIAIRIEDLQSF-KINRQHFIFAIDHDVKALYGRHDDDLQEFK 1483
            PN  +  IG + G+  E  ++ +        P +F++  G+   +G+L YGPPG GKTL A+ I     A    I  GP +L  + GESEAN+R +F  A         Q+A  ++ FDE+D+I K+RG   G      D V+NQLL +MDG+    N+ IIG TNR D+ID A+LRPGR+   I +PLP +  R+QI + + +    K+ +A D+D+  +A +T  +SGA+            I   + A +  A+D++    KG     +D+    +I R+HF  A+ +  +++    D+D+++++
Sbjct:  469 PNVTWEDIGGLQGVKRELQELVQYPVE---HPDKFLK-FGMTPSKGVLFYGPPGCGKTLLAKAIANECQANFISI-KGPELLTMWFGESEANVRDIFDKAR--------QAAPCVLFFDELDSIAKSRGGSVGDGGGAADRVINQLLTEMDGMSAKKNVFIIGATNRPDIIDAAILRPGRLDQLIYIPLPDEKSRIQILKANLR----KSPIAKDVDVNYMAKVTHGFSGADLTEICQRACKQAIRECIEADIRAARDQQL---KGGEPMEDDIDPVPEITRKHFEEAMKYARRSVT---DNDVRKYE 753          
BLAST of Vesicle-fusing ATPase vs. Planmine SMEST
Match: SMESG000032352.1 (SMESG000032352.1)

HSP 1 Score: 153.295 bits (386), Expect = 2.836e-40
Identity = 93/231 (40.26%), Postives = 136/231 (58.87%), Query Frame = 2
Query:  632 VGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKAR--GNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAA 1318
            +GGLD++  +I +   LS +  PE  E LGI H +G+LLYGPPGTGKTL AR +    +    + V+G  ++ KY+GE    +R +F        KM  + +  II  DEID+I  +R  G     S VQ T++ +LL ++DG +  NNI II  TNR D++D ALLRPGR+  +IE P P++  RL+I +IH++++  K      IDL+ +A      SGAE++ V   A
Sbjct:  138 IGGLDKQIQEIKEVIELS-IKHPELFESLGISHPKGVLLYGPPGTGKTLVARAVAHHTDCSFIR-VSGSELVQKYIGEGARMVRDIF--------KMAKEHSPAIIFMDEIDSIGSSRNGGGQGSDSEVQRTML-ELLNQLDGFDATNNIKIIMATNRIDILDSALLRPGRIDRKIEFPEPNEESRLEILKIHSRRMNMKR----GIDLRLIAEKLSGASGAEMKGVCTEA 353          
BLAST of Vesicle-fusing ATPase vs. Planmine SMEST
Match: SMESG000016085.1 (SMESG000016085.1)

HSP 1 Score: 159.458 bits (402), Expect = 3.285e-40
Identity = 95/230 (41.30%), Postives = 133/230 (57.83%), Query Frame = 2
Query:  632 VGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGN-LSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAA 1318
            VGGLD    ++ +        P +F++  G+   +G+L YGPPG GKTL A+ I     A    I  GP +L  + GESEANIR+LF  A         Q+A  I+ FDE+D+I K+RG+  S G    D V+NQLL +MDG+    N+ IIG TNR D+ID A+LRPGR+   I +PLP +  R+QIF    K    K+ +A D+DL  L+ +T  +SGA++  +   A
Sbjct:  488 VGGLDNVKKELQELVQYPVEHPDKFLK-FGMTPSKGVLFYGPPGCGKTLLAKAIASECQANFISI-KGPELLTMWFGESEANIRELFDKAR--------QAAPCILFFDELDSIAKSRGSSASDGGGASDRVINQLLTEMDGMSLKKNVFIIGATNRPDIIDSAILRPGRLDQLIYIPLPDEKSRIQIF----KAALRKSPIAKDVDLNYLSKVTHGFSGADLTEICQRA 703          

HSP 2 Score: 158.688 bits (400), Expect = 6.389e-40
Identity = 141/463 (30.45%), Postives = 225/463 (48.60%), Query Frame = 2
Query:  617 FNSIG---VGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLYGPPGTGKTLTARQIGKMLNAREPK----IVNGPSILDKYVGESEANIRKLFADAEEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMDGVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEIHTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEKFD--PKKGIAIRIEDLQSFKINRQHFIFAID-HDVKAL--------------YGRHDDDLQEFKENRLLKWSPQISKALSTAGLLIESLKTNDNISRKRLLIYGEPLTGTSSVAVHLAKETGFTFIRMCS---MRNMIGFSEPARCLAIKKIFDDALKAPASVIILDDVERLI----DYSSIGPRFSIFLVNNLIGMINCFVPKNKKMLIIGTT 1912
             N IG   +GG  ++ +QI +   L  L  P+  + +G+K   GILLYGPPGTGKTL AR +     A E      ++NGP I+ K  GESE+N+RK F +AE        +++  II  DEIDAI   R    G   V+  +V+QLL  MDG++Q ++++++  TNR + ID AL R GR   ++++ +P   GRL+I  IHTK +R    L +++DL+ +A  T  + GA++ A+   A       K D    +   I  E L S  +  + F +A+D  +  AL               G  D+  +E +E  L+++  +        G+             K +L YG P  G + +A  +A E    FI +     +    G SE      I+++FD A +A   ++  D+++ +       +S G   S  ++N L+  ++    K K + IIG T
Sbjct:  207 LNEIGYDDIGGCRKQLAQIKEMVELP-LRHPQLFKAIGVKPPSGILLYGPPGTGKTLIARAV-----ANETGSFFFLINGPEIMSKLAGESESNLRKAFEEAE--------KNSPAIIFIDEIDAIAPKREKTQG--EVERRIVSQLLTLMDGLKQRSHVVVMAATNRPNSIDSALRRFGRFDREVDIGIPDSIGRLEILRIHTKNMR----LVENVDLEIIAKETHGHVGADLAALCSEAALQQIRNKMDLIDLEEEEIDAEVLNSLAVTMEDFRWALDKSNPSALRETTVEIPNVLWSDVGGLDNVKKELQE--LVQYPVEHPDKFLKFGM----------TPSKGVLFYGPPGCGKTLLAKAIASECQANFISIKGPELLTMWFGESE----ANIRELFDKARQAAPCILFFDELDSIAKSRGSSASDGGGASDRVINQLLTEMDGMSLK-KNVFIIGAT 632          
The following BLAST results are available for this feature:
BLAST of Vesicle-fusing ATPase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
NSF0.000e+044.18N-ethylmaleimide sensitive factor, vesicle fusing ... [more]
NSF0.000e+044.18N-ethylmaleimide sensitive factor, vesicle fusing ... [more]
NSF0.000e+044.18N-ethylmaleimide sensitive factor, vesicle fusing ... [more]
NSF0.000e+044.18N-ethylmaleimide sensitive factor, vesicle fusing ... [more]
NSF0.000e+044.18N-ethylmaleimide sensitive factor, vesicle fusing ... [more]
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BLAST of Vesicle-fusing ATPase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
nsf-10.000e+042.17Vesicle-fusing ATPase [Source:UniProtKB/Swiss-Pro... [more]
nsf-10.000e+042.17Vesicle-fusing ATPase [Source:UniProtKB/Swiss-Pro... [more]
nsf-10.000e+042.17Vesicle-fusing ATPase [Source:UniProtKB/Swiss-Pro... [more]
cdc-48.15.564e-3935.60Transitional endoplasmic reticulum ATPase homolog ... [more]
cdc-48.32.192e-3736.84ATPase family protein 2 homolog [Source:UniProtKB... [more]
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BLAST of Vesicle-fusing ATPase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
comt0.000e+044.92gene:FBgn0000346 transcript:FBtr0073754[more]
comt0.000e+044.92gene:FBgn0000346 transcript:FBtr0333329[more]
Nsf20.000e+043.30gene:FBgn0266464 transcript:FBtr0344766[more]
Nsf20.000e+043.30gene:FBgn0266464 transcript:FBtr0344767[more]
Nsf20.000e+043.30gene:FBgn0266464 transcript:FBtr0082883[more]
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BLAST of Vesicle-fusing ATPase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
nsfa0.000e+042.78N-ethylmaleimide-sensitive factor a [Source:ZFIN;A... [more]
nsfb0.000e+043.84N-ethylmaleimide-sensitive factor b [Source:ZFIN;A... [more]
nsfb0.000e+044.16N-ethylmaleimide-sensitive factor b [Source:ZFIN;A... [more]
nsfb0.000e+044.16N-ethylmaleimide-sensitive factor b [Source:ZFIN;A... [more]
nsfb0.000e+044.16N-ethylmaleimide-sensitive factor b [Source:ZFIN;A... [more]
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BLAST of Vesicle-fusing ATPase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
fosl20.000e+041.39FOS-like antigen 2 [Source:Xenbase;Acc:XB-GENE-625... [more]
fosl20.000e+041.51FOS-like antigen 2 [Source:Xenbase;Acc:XB-GENE-625... [more]
fosl20.000e+041.34FOS-like antigen 2 [Source:Xenbase;Acc:XB-GENE-625... [more]
fosl20.000e+041.51FOS-like antigen 2 [Source:Xenbase;Acc:XB-GENE-625... [more]
fosl20.000e+041.51FOS-like antigen 2 [Source:Xenbase;Acc:XB-GENE-625... [more]
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BLAST of Vesicle-fusing ATPase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Nsf0.000e+044.48N-ethylmaleimide sensitive fusion protein [Source:... [more]
Vcp2.135e-4130.94valosin containing protein [Source:MGI Symbol;Acc:... [more]
Psmc51.001e-3839.33protease (prosome, macropain) 26S subunit, ATPase ... [more]
Psmc11.362e-3838.96protease (prosome, macropain) 26S subunit, ATPase ... [more]
Spata57.422e-3839.36spermatogenesis associated 5 [Source:MGI Symbol;Ac... [more]
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BLAST of Vesicle-fusing ATPase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P46461|NSF1_DROME0.000e+044.92Vesicle-fusing ATPase 1 OS=Drosophila melanogaster... [more]
sp|P54351|NSF2_DROME0.000e+043.30Vesicle-fusing ATPase 2 OS=Drosophila melanogaster... [more]
sp|Q9QUL6|NSF_RAT0.000e+044.48Vesicle-fusing ATPase OS=Rattus norvegicus OX=1011... [more]
sp|P46460|NSF_MOUSE0.000e+044.48Vesicle-fusing ATPase OS=Mus musculus OX=10090 GN=... [more]
sp|P18708|NSF_CRIGR0.000e+044.33Vesicle-fusing ATPase OS=Cricetulus griseus OX=100... [more]
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BLAST of Vesicle-fusing ATPase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
C1LF610.000e+043.59NEM-sensitive fusion protein 2 OS=Schistosoma japo... [more]
A0A0N4ZGZ30.000e+045.11Uncharacterized protein OS=Parastrongyloides trich... [more]
A0A0K0DX310.000e+044.67Uncharacterized protein OS=Strongyloides stercoral... [more]
G4VMW90.000e+043.18Putative vesicular-fusion protein nsf OS=Schistoso... [more]
A0A267EGJ40.000e+042.68Uncharacterized protein (Fragment) OS=Macrostomum ... [more]
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BLAST of Vesicle-fusing ATPase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
nsfa0.000e+044.38vesicle-fusing ATPase [Source:NCBI gene;Acc:103037... [more]
nsfa0.000e+044.38vesicle-fusing ATPase [Source:NCBI gene;Acc:103037... [more]
nsfa0.000e+044.38vesicle-fusing ATPase [Source:NCBI gene;Acc:103037... [more]
nsfb0.000e+043.46N-ethylmaleimide-sensitive factor b [Source:ZFIN;A... [more]
nsfa0.000e+043.70vesicle-fusing ATPase [Source:NCBI gene;Acc:103037... [more]
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BLAST of Vesicle-fusing ATPase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
nsfa1.674e-11336.10N-ethylmaleimide-sensitive factor a [Source:ZFIN;A... [more]
psmc54.034e-3939.33proteasome 26S subunit, ATPase 5 [Source:ZFIN;Acc:... [more]
ENSPMAT00000010299.12.247e-3535.93pep scaffold:Pmarinus_7.0:GL479893:343:6144:1 gene... [more]
ENSPMAT00000001244.17.102e-3537.97pep scaffold:Pmarinus_7.0:GL482260:6416:9764:-1 ge... [more]
nvl9.547e-3437.65nuclear VCP like [Source:ZFIN;Acc:ZDB-GENE-040426-... [more]
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BLAST of Vesicle-fusing ATPase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 5
Match NameE-valueIdentityDescription
SEC182.245e-16337.30AAA ATPase and SNARE disassembly chaperone; requir... [more]
CDC481.448e-4632.97AAA ATPase; subunit of polyUb-selective segregase ... [more]
AFG21.040e-3830.35ATPase of the CDC48/PAS1/SEC18 (AAA) family, forms... [more]
RPT64.479e-3839.33ATPase of the 19S regulatory particle of the 26S p... [more]
PEX65.969e-3838.62AAA-peroxin; heterodimerizes with AAA-peroxin Pex1... [more]
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BLAST of Vesicle-fusing ATPase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO362240.000e+042.18Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO390062.162e-4240.60Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO362186.376e-4130.40Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO495245.162e-3940.17Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO374731.351e-3436.71Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Vesicle-fusing ATPase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
NSF0.000e+043.71vesicle-fusing ATPase [Source:NCBI gene;Acc:101155... [more]
NSF0.000e+043.71vesicle-fusing ATPase [Source:NCBI gene;Acc:101155... [more]
NSF0.000e+043.71vesicle-fusing ATPase [Source:NCBI gene;Acc:101155... [more]
nsfb0.000e+041.96vesicle-fusing ATPase [Source:NCBI gene;Acc:101174... [more]
nsfb2.469e-13845.12vesicle-fusing ATPase [Source:NCBI gene;Acc:101174... [more]
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BLAST of Vesicle-fusing ATPase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000019983.10.000e+0100.00SMESG000019983.1[more]
SMESG000047180.11.453e-4141.84SMESG000047180.1[more]
SMESG000009924.16.581e-4130.37SMESG000009924.1[more]
SMESG000032352.12.836e-4040.26SMESG000032352.1[more]
SMESG000016085.13.285e-4041.30SMESG000016085.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30019484 ID=SMED30019484|Name=Vesicle-fusing ATPase|organism=Schmidtea mediterranea sexual|type=transcript|length=2372bp
AATGATTGCGTTTAAAGTTCAAAAAAATTCTTATGATGAATTTTCGTATA
CAAATAAAATATTTTTTAATCCTAGCGATTGGGCATTGTTTAAAGAAAAG
TTTATAACTGTAACAACGGCTTCGAATAAATTCATTTTCAAATCCGACAA
AGATGAAAGTGTTAAAAAAGGCTGTATGACAACTAACGGAATACAAAGAA
AATGGATGTATTGTGGAATTGGTGAAGTTGTTGAGTGCATTCCATATTCA
TTCCAAACAGTAGTACTCGTAACTGAAGTCATTGTGGAGTTGGATTTATT
TGTTAAATCTGCGTCATTGAAAGATCCAATTGATTCAGACAAATTGGCAG
TGAAATATCTGGAACGCTTTCATGATACACCGGTTACAAAAAATCAATTA
CTTTTATTTAACTGGGAAAGCATTAATTTTGAAGCAAAGATTATCAGCAT
TCAAGGTTTGGATGTAAAGTCAAGCAAAATGAATGAGATCGAAACTGGAA
TAATCCAATCGAATACGGTATTCAAGTTCCAACGAAGACCGGAATCAAAA
TTTATGCTATCTGGACGATGCAAAGCAGAAAATGCTGCAGTATGCTTAAT
AAATCCAAATTGGGATTTCAACAGCATTGGAGTCGGCGGGCTCGATGAGG
AATTCTCGCAAATATTCAAATCTGCTTTTCTATCACGTCTGTTTCCACCT
GAATTCGTCGAGGAATTAGGAATAAAACATGTCCGCGGCATTCTTTTATA
CGGTCCTCCTGGGACTGGGAAAACTTTAACAGCTCGCCAGATTGGGAAAA
TGCTGAATGCCCGAGAGCCGAAAATTGTGAATGGGCCTTCAATTTTGGAT
AAATACGTCGGTGAGTCCGAGGCAAACATCAGGAAACTCTTTGCTGATGC
CGAGGAAGAATTTAAGAAGATGGGAAACCAGTCGGCGTTGCATATCATAA
TTTTTGATGAAATCGACGCCATTTGCAAGGCTCGAGGAAATCTGTCGGGT
GGATCAGCTGTGCAAGACACAGTAGTCAATCAGCTGCTTGCTAAAATGGA
CGGCGTGGAGCAGCTGAATAACATATTGATCATCGGCATGACAAACAGGC
GCGACATGATAGACGAGGCCTTGTTGCGACCAGGTCGCATGGGCGTCCAA
ATTGAGGTGCCGTTGCCGAGTCAGCACGGCCGGTTGCAAATATTTGAGAT
CCATACAAAGAAATTGAGAGAGAAAAACATCCTGGCAGACGATATCGATT
TGAAAGAATTGGCAGCATTGACAAAAAACTACAGCGGAGCCGAAATCGAG
GCAGTTGTCATGGCGGCGTTGGCAAATGCACAGGACGAGAAATTTGACCC
GAAAAAAGGAATCGCCATTCGCATAGAAGATTTGCAATCATTCAAAATAA
ATCGCCAGCATTTCATTTTTGCCATCGATCACGACGTGAAAGCGCTTTAC
GGGCGGCATGATGATGATTTGCAGGAATTCAAGGAGAATCGGCTGCTGAA
ATGGAGCCCGCAAATATCCAAAGCACTCTCAACGGCCGGATTATTGATTG
AAAGCCTGAAAACCAATGACAATATCAGTCGGAAGCGGTTATTAATCTAC
GGAGAGCCGCTTACAGGAACGTCATCGGTTGCCGTGCATTTGGCTAAGGA
AACCGGGTTTACATTCATTCGGATGTGCTCTATGAGGAACATGATCGGGT
TCAGTGAGCCTGCTCGGTGCCTCGCTATCAAAAAGATATTCGATGACGCG
CTGAAAGCGCCCGCCAGCGTGATCATTCTGGACGACGTGGAACGGCTGAT
CGACTACTCAAGCATCGGACCGAGATTCTCGATATTCCTCGTCAATAACC
TCATCGGAATGATCAATTGCTTTGTGCCCAAGAACAAAAAGATGCTGATC
ATCGGCACCACCAAGCATTTGTCTGCCATGCACGAATTGGAATTCTTTTC
CGCGTTCGACAAGAACTGTATCAAAATCCCGGAAATCAAAACCCCCTCGG
AAATAATCTCGGTATTGGCGGATCAGGATCTCTACAGCGATTCGGATCTG
CAGCAGATCGATAATCATTTGCAGATGGGCGACTGCTCGGATCTGGCGAT
CGGGATCAAGAATTTGATGGAGAATTGCAAGGATCTGAAATCGGTCGATC
CGGCAAGGCGAGTTGACGAGTTGCTGATGAAACTGAAATCTTCTTCGGAA
TTTCAATTTTGAATAAATTTTGTGATTTATTATTTGTTGCCTAGCAACTA
GTTTCCATAGCGTTCCTAATAAAGCATAATTTCCTCGTTCAACAGATTGC
ATTTATTATTAGTTATTTTGATTTTATTATATATTTAATATTTGGCAGAT
TGATAATATAAAATATTTCGTC
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protein sequence of SMED30019484-orf-1

>SMED30019484-orf-1 ID=SMED30019484-orf-1|Name=SMED30019484-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=165bp
MQSVERGNYALLGTLWKLVARQQIINHKIYSKLKFRRRFQFHQQLVNSPC
RIDRFQILAILHQILDPDRQIRAVAHLQMIIDLLQIRIAVEILIRQYRDY
FRGGFDFRDFDTVLVERGKEFQFVHGRQMLGGADDQHLFVLGHKAIDHSD
EVIDEEYRESRSDA*
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protein sequence of SMED30019484-orf-2

>SMED30019484-orf-2 ID=SMED30019484-orf-2|Name=SMED30019484-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=737bp
MIAFKVQKNSYDEFSYTNKIFFNPSDWALFKEKFITVTTASNKFIFKSDK
DESVKKGCMTTNGIQRKWMYCGIGEVVECIPYSFQTVVLVTEVIVELDLF
VKSASLKDPIDSDKLAVKYLERFHDTPVTKNQLLLFNWESINFEAKIISI
QGLDVKSSKMNEIETGIIQSNTVFKFQRRPESKFMLSGRCKAENAAVCLI
NPNWDFNSIGVGGLDEEFSQIFKSAFLSRLFPPEFVEELGIKHVRGILLY
GPPGTGKTLTARQIGKMLNAREPKIVNGPSILDKYVGESEANIRKLFADA
EEEFKKMGNQSALHIIIFDEIDAICKARGNLSGGSAVQDTVVNQLLAKMD
GVEQLNNILIIGMTNRRDMIDEALLRPGRMGVQIEVPLPSQHGRLQIFEI
HTKKLREKNILADDIDLKELAALTKNYSGAEIEAVVMAALANAQDEKFDP
KKGIAIRIEDLQSFKINRQHFIFAIDHDVKALYGRHDDDLQEFKENRLLK
WSPQISKALSTAGLLIESLKTNDNISRKRLLIYGEPLTGTSSVAVHLAKE
TGFTFIRMCSMRNMIGFSEPARCLAIKKIFDDALKAPASVIILDDVERLI
DYSSIGPRFSIFLVNNLIGMINCFVPKNKKMLIIGTTKHLSAMHELEFFS
AFDKNCIKIPEIKTPSEIISVLADQDLYSDSDLQQIDNHLQMGDCSDLAI
GIKNLMENCKDLKSVDPARRVDELLMKLKSSSEFQF*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: cellular component
TermDefinition
GO:0030496midbody
GO:0005737cytoplasm
GO:0005765lysosomal membrane
GO:0005829cytosol
GO:0005886plasma membrane
GO:0043209myelin sheath
GO:0070062extracellular exosome
Vocabulary: molecular function
TermDefinition
GO:0042802identical protein binding
GO:0044877macromolecular complex binding
GO:0005524ATP binding
GO:0000149SNARE binding
GO:0000166nucleotide binding
GO:0005515protein binding
GO:0016787hydrolase activity
GO:0016887ATPase activity
GO:0017075syntaxin-1 binding
GO:0019901protein kinase binding
GO:0019905syntaxin binding
GO:0030165PDZ domain binding
GO:0032403protein complex binding
GO:0035255ionotropic glutamate receptor binding
GO:0042623ATPase activity, coupled
GO:0046872metal ion binding
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
Vocabulary: INTERPRO
TermDefinition
IPR041569AAA_lid_3
IPR039812Vesicle-fus_ATPase
IPR003593AAA+_ATPase
IPR027417P-loop_NTPase
IPR029067CDC48_domain_2-like_sf
IPR003959ATPase_AAA_core
IPR003960ATPase_AAA_CS
IPR009010Asp_de-COase-like_dom_sf
Vocabulary: biological process
TermDefinition
GO:0001921positive regulation of receptor recycling
GO:0006810transport
GO:0006813potassium ion transport
GO:0006886intracellular protein transport
GO:0015031protein transport
GO:0035494SNARE complex disassembly
GO:0045732positive regulation of protein catabolic process
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003593AAA+ ATPase domainSMARTSM00382AAA_5coord: 526..731
e-value: 1.3
score: 12.4
coord: 243..390
e-value: 1.4E-16
score: 71.1
IPR041569AAA ATPase, AAA+ lid domainPFAMPF17862AAA_lid_3coord: 414..452
e-value: 1.5E-10
score: 40.7
NoneNo IPR availableGENE3DG3DSA:3.40.50.300coord: 200..388
e-value: 8.1E-50
score: 171.0
NoneNo IPR availableGENE3DG3DSA:3.10.330.10coord: 85..186
e-value: 2.2E-11
score: 45.9
NoneNo IPR availableGENE3DG3DSA:3.40.50.300coord: 488..660
e-value: 4.2E-23
score: 83.9
NoneNo IPR availableGENE3DG3DSA:1.10.8.60coord: 389..479
e-value: 1.9E-15
score: 58.5
NoneNo IPR availableGENE3DG3DSA:2.40.40.20coord: 2..82
e-value: 2.3E-12
score: 48.5
NoneNo IPR availableCDDcd00009AAAcoord: 526..654
e-value: 7.88321E-4
score: 38.6663
NoneNo IPR availableCDDcd00009AAAcoord: 245..388
e-value: 6.34584E-22
score: 90.6683
IPR003959ATPase, AAA-type, corePFAMPF00004AAAcoord: 530..643
e-value: 3.7E-8
score: 33.9
coord: 247..388
e-value: 2.1E-39
score: 135.0
IPR039812Vesicle-fusing ATPasePANTHERPTHR23078VESICULAR-FUSION PROTEIN NSFcoord: 10..709
IPR003960ATPase, AAA-type, conserved sitePROSITEPS00674AAAcoord: 358..376
IPR009010Aspartate decarboxylase-like domain superfamilySUPERFAMILYSSF50692ADC-likecoord: 3..85
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILYSSF52540P-loop containing nucleoside triphosphate hydrolasescoord: 211..480
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILYSSF52540P-loop containing nucleoside triphosphate hydrolasescoord: 495..716
IPR029067CDC48 domain 2-like superfamilySUPERFAMILYSSF54585Cdc48 domain 2-likecoord: 90..189