Glucosidase 2 subunit beta

Overview
NameGlucosidase 2 subunit beta
Smed IDSMED30019477
Length (bp)1680
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Glucosidase 2 subunit beta (SMED30019477) t-SNE clustered cells

Violin plots show distribution of expression levels for Glucosidase 2 subunit beta (SMED30019477) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Glucosidase 2 subunit beta (SMED30019477) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Glucosidase 2 subunit beta (SMED30019477) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30019477

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
Smed sexual biotypeSMED30019477h1SMcG0009681 Contig54565uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30019477h1SMcG0009681 Contig54565newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
gutSMED30019477h1SMcG0009681 dd_Smed_v4_1730_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30019477h1SMcG0009681 dd_Smed_v4_1730_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30019477h1SMcG0009681 dd_Smed_v4_1730_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30019477h1SMcG0009681 dd_Smed_v4_1730_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30019477h1SMcG0009681 dd_Smed_v6_1730_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
secretory systemSMED30019477h1SMcG0009681 dd_Smed_v6_1730_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Glucosidase 2 subunit beta vs. Ensembl Human
Match: PRKCSH (protein kinase C substrate 80K-H [Source:HGNC Symbol;Acc:HGNC:9411])

HSP 1 Score: 211.46 bits (537), Expect = 6.297e-61
Identity = 104/265 (39.25%), Postives = 159/265 (60.00%), Query Frame = 1
Query:   94 VDKPRGIPFSKLSFYQPNSPFKCLDGSGVISWMKVNDDYCDCRDATDEPGTSACSNGVFHCDNKGHKPQIIPSSRVNDGICDCCDGQDEYSGIINCTNTCWELGAIAREEMKRVKEAAEKGHKIYQEYIKQYETSVSEKRSRLETINEQFKVLSEEKLVSERSKTDSENNESEAKRVFEENWKKLQSENETKLERYKISKAFNELDIDVNERVTFEELQSHHEFDIDQNGDVSPEEAKEYLSERESVNEIEFTEIVWPNIKQIFK 888
            V +PRG+  +   FY  + PF CLDGS  I + +VNDDYCDC+D +DEPGT+AC NG FHC N G+KP  IPS+RVNDG+CDCCDG DEY+  + C NTC E G   RE ++++ E   +G ++ +  I+ ++ +  EK+ +L  +    K L ++  +    K ++E  E EAK   ++ W++  +  + + E+   + AF ELD D++  V+  ELQ+H E D D +G +S  EA+  LS     +   F + VW  I+  ++
Sbjct:   17 VKRPRGVSLTNHHFYDESKPFTCLDGSATIPFDQVNDDYCDCKDGSDEPGTAACPNGSFHCTNTGYKPLYIPSNRVNDGVCDCCDGTDEYNSGVICENTCKEKGRKERESLQQMAEVTREGFRLKKILIEDWKKAREEKQKKLIELQAGKKSLEDQVEMLRTVKEEAEKPEREAKEQHQKLWEEQLAAAKAQQEQELAADAFKELDDDMDGTVSVTELQTHPELDTDGDGALSEAEAQALLSGDTQTDATSFYDRVWAAIRDKYR 281          

HSP 2 Score: 114.775 bits (286), Expect = 1.771e-26
Identity = 58/139 (41.73%), Postives = 79/139 (56.83%), Query Frame = 3
Query: 1116 KSIENLLNIDYGPSSSFYKLAEEDTCYELTDREYIYKLCPFKKTSQMSKDSGPETELGKWGHWDG-EENQYSMQVYDNGLPCWNGPNRQTKVMIYCGVENKLTEASEPSRCEYLFKF---ETPSACDKSSEQLNDHDEL 1520
            +++E  ++ D+GP+  F  L  +  CYELT  EY+Y+LCPFK  SQ  K  G  T LG WG W G + +++S   Y+ G  CW GPNR T V + CG E  +T  +EPSRCEYL +             +   +DHDEL
Sbjct:  389 RNLEQEISFDFGPNGEFAYLYSQ--CYELTTNEYVYRLCPFKLVSQKPKLGGSPTSLGTWGSWIGPDHDKFSAMKYEQGTGCWQGPNRSTTVRLLCGKETMVTSTTEPSRCEYLMELMTPAACPEPPPEAPTEDDHDEL 525          
BLAST of Glucosidase 2 subunit beta vs. Ensembl Human
Match: PRKCSH (protein kinase C substrate 80K-H [Source:HGNC Symbol;Acc:HGNC:9411])

HSP 1 Score: 211.46 bits (537), Expect = 6.297e-61
Identity = 104/265 (39.25%), Postives = 159/265 (60.00%), Query Frame = 1
Query:   94 VDKPRGIPFSKLSFYQPNSPFKCLDGSGVISWMKVNDDYCDCRDATDEPGTSACSNGVFHCDNKGHKPQIIPSSRVNDGICDCCDGQDEYSGIINCTNTCWELGAIAREEMKRVKEAAEKGHKIYQEYIKQYETSVSEKRSRLETINEQFKVLSEEKLVSERSKTDSENNESEAKRVFEENWKKLQSENETKLERYKISKAFNELDIDVNERVTFEELQSHHEFDIDQNGDVSPEEAKEYLSERESVNEIEFTEIVWPNIKQIFK 888
            V +PRG+  +   FY  + PF CLDGS  I + +VNDDYCDC+D +DEPGT+AC NG FHC N G+KP  IPS+RVNDG+CDCCDG DEY+  + C NTC E G   RE ++++ E   +G ++ +  I+ ++ +  EK+ +L  +    K L ++  +    K ++E  E EAK   ++ W++  +  + + E+   + AF ELD D++  V+  ELQ+H E D D +G +S  EA+  LS     +   F + VW  I+  ++
Sbjct:   17 VKRPRGVSLTNHHFYDESKPFTCLDGSATIPFDQVNDDYCDCKDGSDEPGTAACPNGSFHCTNTGYKPLYIPSNRVNDGVCDCCDGTDEYNSGVICENTCKEKGRKERESLQQMAEVTREGFRLKKILIEDWKKAREEKQKKLIELQAGKKSLEDQVEMLRTVKEEAEKPEREAKEQHQKLWEEQLAAAKAQQEQELAADAFKELDDDMDGTVSVTELQTHPELDTDGDGALSEAEAQALLSGDTQTDATSFYDRVWAAIRDKYR 281          

HSP 2 Score: 114.775 bits (286), Expect = 1.771e-26
Identity = 58/139 (41.73%), Postives = 79/139 (56.83%), Query Frame = 3
Query: 1116 KSIENLLNIDYGPSSSFYKLAEEDTCYELTDREYIYKLCPFKKTSQMSKDSGPETELGKWGHWDG-EENQYSMQVYDNGLPCWNGPNRQTKVMIYCGVENKLTEASEPSRCEYLFKF---ETPSACDKSSEQLNDHDEL 1520
            +++E  ++ D+GP+  F  L  +  CYELT  EY+Y+LCPFK  SQ  K  G  T LG WG W G + +++S   Y+ G  CW GPNR T V + CG E  +T  +EPSRCEYL +             +   +DHDEL
Sbjct:  389 RNLEQEISFDFGPNGEFAYLYSQ--CYELTTNEYVYRLCPFKLVSQKPKLGGSPTSLGTWGSWIGPDHDKFSAMKYEQGTGCWQGPNRSTTVRLLCGKETMVTSTTEPSRCEYLMELMTPAACPEPPPEAPTEDDHDEL 525          
BLAST of Glucosidase 2 subunit beta vs. Ensembl Human
Match: PRKCSH (protein kinase C substrate 80K-H [Source:HGNC Symbol;Acc:HGNC:9411])

HSP 1 Score: 211.46 bits (537), Expect = 7.444e-61
Identity = 104/265 (39.25%), Postives = 159/265 (60.00%), Query Frame = 1
Query:   94 VDKPRGIPFSKLSFYQPNSPFKCLDGSGVISWMKVNDDYCDCRDATDEPGTSACSNGVFHCDNKGHKPQIIPSSRVNDGICDCCDGQDEYSGIINCTNTCWELGAIAREEMKRVKEAAEKGHKIYQEYIKQYETSVSEKRSRLETINEQFKVLSEEKLVSERSKTDSENNESEAKRVFEENWKKLQSENETKLERYKISKAFNELDIDVNERVTFEELQSHHEFDIDQNGDVSPEEAKEYLSERESVNEIEFTEIVWPNIKQIFK 888
            V +PRG+  +   FY  + PF CLDGS  I + +VNDDYCDC+D +DEPGT+AC NG FHC N G+KP  IPS+RVNDG+CDCCDG DEY+  + C NTC E G   RE ++++ E   +G ++ +  I+ ++ +  EK+ +L  +    K L ++  +    K ++E  E EAK   ++ W++  +  + + E+   + AF ELD D++  V+  ELQ+H E D D +G +S  EA+  LS     +   F + VW  I+  ++
Sbjct:   17 VKRPRGVSLTNHHFYDESKPFTCLDGSATIPFDQVNDDYCDCKDGSDEPGTAACPNGSFHCTNTGYKPLYIPSNRVNDGVCDCCDGTDEYNSGVICENTCKEKGRKERESLQQMAEVTREGFRLKKILIEDWKKAREEKQKKLIELQAGKKSLEDQVEMLRTVKEEAEKPEREAKEQHQKLWEEQLAAAKAQQEQELAADAFKELDDDMDGTVSVTELQTHPELDTDGDGALSEAEAQALLSGDTQTDATSFYDRVWAAIRDKYR 281          

HSP 2 Score: 114.775 bits (286), Expect = 2.019e-26
Identity = 58/139 (41.73%), Postives = 79/139 (56.83%), Query Frame = 3
Query: 1116 KSIENLLNIDYGPSSSFYKLAEEDTCYELTDREYIYKLCPFKKTSQMSKDSGPETELGKWGHWDG-EENQYSMQVYDNGLPCWNGPNRQTKVMIYCGVENKLTEASEPSRCEYLFKF---ETPSACDKSSEQLNDHDEL 1520
            +++E  ++ D+GP+  F  L  +  CYELT  EY+Y+LCPFK  SQ  K  G  T LG WG W G + +++S   Y+ G  CW GPNR T V + CG E  +T  +EPSRCEYL +             +   +DHDEL
Sbjct:  392 RNLEQEISFDFGPNGEFAYLYSQ--CYELTTNEYVYRLCPFKLVSQKPKLGGSPTSLGTWGSWIGPDHDKFSAMKYEQGTGCWQGPNRSTTVRLLCGKETMVTSTTEPSRCEYLMELMTPAACPEPPPEAPTEDDHDEL 528          
BLAST of Glucosidase 2 subunit beta vs. Ensembl Human
Match: PRKCSH (protein kinase C substrate 80K-H [Source:HGNC Symbol;Acc:HGNC:9411])

HSP 1 Score: 211.46 bits (537), Expect = 8.027e-61
Identity = 104/265 (39.25%), Postives = 159/265 (60.00%), Query Frame = 1
Query:   94 VDKPRGIPFSKLSFYQPNSPFKCLDGSGVISWMKVNDDYCDCRDATDEPGTSACSNGVFHCDNKGHKPQIIPSSRVNDGICDCCDGQDEYSGIINCTNTCWELGAIAREEMKRVKEAAEKGHKIYQEYIKQYETSVSEKRSRLETINEQFKVLSEEKLVSERSKTDSENNESEAKRVFEENWKKLQSENETKLERYKISKAFNELDIDVNERVTFEELQSHHEFDIDQNGDVSPEEAKEYLSERESVNEIEFTEIVWPNIKQIFK 888
            V +PRG+  +   FY  + PF CLDGS  I + +VNDDYCDC+D +DEPGT+AC NG FHC N G+KP  IPS+RVNDG+CDCCDG DEY+  + C NTC E G   RE ++++ E   +G ++ +  I+ ++ +  EK+ +L  +    K L ++  +    K ++E  E EAK   ++ W++  +  + + E+   + AF ELD D++  V+  ELQ+H E D D +G +S  EA+  LS     +   F + VW  I+  ++
Sbjct:   17 VKRPRGVSLTNHHFYDESKPFTCLDGSATIPFDQVNDDYCDCKDGSDEPGTAACPNGSFHCTNTGYKPLYIPSNRVNDGVCDCCDGTDEYNSGVICENTCKEKGRKERESLQQMAEVTREGFRLKKILIEDWKKAREEKQKKLIELQAGKKSLEDQVEMLRTVKEEAEKPEREAKEQHQKLWEEQLAAAKAQQEQELAADAFKELDDDMDGTVSVTELQTHPELDTDGDGALSEAEAQALLSGDTQTDATSFYDRVWAAIRDKYR 281          

HSP 2 Score: 114.775 bits (286), Expect = 1.985e-26
Identity = 58/139 (41.73%), Postives = 79/139 (56.83%), Query Frame = 3
Query: 1116 KSIENLLNIDYGPSSSFYKLAEEDTCYELTDREYIYKLCPFKKTSQMSKDSGPETELGKWGHWDG-EENQYSMQVYDNGLPCWNGPNRQTKVMIYCGVENKLTEASEPSRCEYLFKF---ETPSACDKSSEQLNDHDEL 1520
            +++E  ++ D+GP+  F  L  +  CYELT  EY+Y+LCPFK  SQ  K  G  T LG WG W G + +++S   Y+ G  CW GPNR T V + CG E  +T  +EPSRCEYL +             +   +DHDEL
Sbjct:  399 RNLEQEISFDFGPNGEFAYLYSQ--CYELTTNEYVYRLCPFKLVSQKPKLGGSPTSLGTWGSWIGPDHDKFSAMKYEQGTGCWQGPNRSTTVRLLCGKETMVTSTTEPSRCEYLMELMTPAACPEPPPEAPTEDDHDEL 535          
BLAST of Glucosidase 2 subunit beta vs. Ensembl Human
Match: PRKCSH (protein kinase C substrate 80K-H [Source:HGNC Symbol;Acc:HGNC:9411])

HSP 1 Score: 159.073 bits (401), Expect = 2.216e-45
Identity = 70/143 (48.95%), Postives = 98/143 (68.53%), Query Frame = 1
Query:   94 VDKPRGIPFSKLSFYQPNSPFKCLDGSGVISWMKVNDDYCDCRDATDEPGTSACSNGVFHCDNKGHKPQIIPSSRVNDGICDCCDGQDEYSGIINCTNTCWELGAIAREEMKRVKEAAEKGHKIYQEYIKQYETSVSEKRSRL 522
            V +PRG+  +   FY  + PF CLDGS  I + +VNDDYCDC+D +DEPGT+AC NG FHC N G+KP  IPS+RVNDG+CDCCDG DEY+  + C NTC E G   RE ++++ E   +G ++ +  I+ ++ +  EK+ +L
Sbjct:   17 VKRPRGVSLTNHHFYDESKPFTCLDGSATIPFDQVNDDYCDCKDGSDEPGTAACPNGSFHCTNTGYKPLYIPSNRVNDGVCDCCDGTDEYNSGVICENTCKEKGRKERESLQQMAEVTREGFRLKKILIEDWKKAREEKQKKL 159          
BLAST of Glucosidase 2 subunit beta vs. Ensembl Celegans
Match: ZK1307.8 (pep chromosome:WBcel235:II:9624590:9626625:1 gene:WBGene00014249.1 transcript:ZK1307.8.4 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:ZK1307.8)

HSP 1 Score: 189.119 bits (479), Expect = 3.630e-53
Identity = 102/274 (37.23%), Postives = 163/274 (59.49%), Query Frame = 1
Query:  106 RGIPFSKLSFYQPNSP--FKCLDGSGVISWMKVNDDYCDCRDATDEPGTSACSNGVFHCDNKGHKPQIIPSSRVNDGICDCCDGQDEYSGIINCTNTCWELGAIAREEMKRVKEAAEKGHKIYQEYIKQYETSVSEKRSRLETINEQFKVLSEEKLVSERSKTDSENNESEAKRVFEENWKKLQSENETKLERYKISKAFNELDIDVNERVTFEELQSHHEFDIDQNGDVSPEEAKEYLSERESVNEIEFTEIVWPNIKQIFKFKERTENDLRH 921
            +G+P S+L+ YQP++   F+CLDGS  I + ++NDDYCDC+D +DEPGTSAC N  F+C N GHK   IP++RVND +CDCCDG DEY   ++C N C ELG  AR E ++V   A KG++  QE  K+ +     K   +E + ++  +L  +++  E  K  +E+ E + +      W+ +++E     ++ + ++ F+ELD+D N ++  EEL+ +   D D +G VS +E K Y +  E+  E  F  +++  +K     K    +D+RH
Sbjct:   28 KGVPHSRLNLYQPSTTDTFRCLDGSQTILYSQLNDDYCDCKDGSDEPGTSACGNAFFYCSNVGHKGNFIPTNRVNDKLCDCCDGSDEYDSGVDCPNICDELGRAARIEHEKVANIARKGYQKRQELAKEGQALRDSKLKDVEPLRQERALLEPDRVKFEGEKKVAEDAERKLQDEHRNQWESVKNEK----KKLRAAEWFDELDLDKNGKIDKEELRQNIFLDDDNDGFVSEDEVKAYHTVDEADLE-HFQSLMYDRLKTA---KRTHLDDIRH 293          

HSP 2 Score: 107.842 bits (268), Expect = 1.094e-24
Identity = 54/136 (39.71%), Postives = 80/136 (58.82%), Query Frame = 3
Query: 1116 KSIENLLNIDYGPSSSFYKLAEEDTCYELTDREYIYKLCPFKKTSQMSKDSGPETELGKWGHWDGEE-NQYSMQVYDNGLPCWNGPNRQTKVMIYCGVENKLTEASEPSRCEYLFKFETPSACDKSSEQLNDHDEL 1520
            +  E+  N DYG  S++  L  +D C++   ++Y Y+ CPF + +Q    +   T LG +  W G E N+YS   + +G  CWNGP R T + I CG EN+L E +EP++CEYLF F TP AC    +++  H+EL
Sbjct:  375 REAEDFANSDYGEDSAWAAL--KDKCFDRNVQQYTYQFCPFGQNTQKDTGAYSGTSLGSFKEWSGPEGNKYSKMHFGDGQQCWNGPKRSTDITIECGEENELVEVTEPAKCEYLFTFRTPLACADPDKEVV-HEEL 507          
BLAST of Glucosidase 2 subunit beta vs. Ensembl Celegans
Match: ZK1307.8 (pep chromosome:WBcel235:II:9624613:9626625:1 gene:WBGene00014249.1 transcript:ZK1307.8.3 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:ZK1307.8)

HSP 1 Score: 189.119 bits (479), Expect = 3.630e-53
Identity = 102/274 (37.23%), Postives = 163/274 (59.49%), Query Frame = 1
Query:  106 RGIPFSKLSFYQPNSP--FKCLDGSGVISWMKVNDDYCDCRDATDEPGTSACSNGVFHCDNKGHKPQIIPSSRVNDGICDCCDGQDEYSGIINCTNTCWELGAIAREEMKRVKEAAEKGHKIYQEYIKQYETSVSEKRSRLETINEQFKVLSEEKLVSERSKTDSENNESEAKRVFEENWKKLQSENETKLERYKISKAFNELDIDVNERVTFEELQSHHEFDIDQNGDVSPEEAKEYLSERESVNEIEFTEIVWPNIKQIFKFKERTENDLRH 921
            +G+P S+L+ YQP++   F+CLDGS  I + ++NDDYCDC+D +DEPGTSAC N  F+C N GHK   IP++RVND +CDCCDG DEY   ++C N C ELG  AR E ++V   A KG++  QE  K+ +     K   +E + ++  +L  +++  E  K  +E+ E + +      W+ +++E     ++ + ++ F+ELD+D N ++  EEL+ +   D D +G VS +E K Y +  E+  E  F  +++  +K     K    +D+RH
Sbjct:   28 KGVPHSRLNLYQPSTTDTFRCLDGSQTILYSQLNDDYCDCKDGSDEPGTSACGNAFFYCSNVGHKGNFIPTNRVNDKLCDCCDGSDEYDSGVDCPNICDELGRAARIEHEKVANIARKGYQKRQELAKEGQALRDSKLKDVEPLRQERALLEPDRVKFEGEKKVAEDAERKLQDEHRNQWESVKNEK----KKLRAAEWFDELDLDKNGKIDKEELRQNIFLDDDNDGFVSEDEVKAYHTVDEADLE-HFQSLMYDRLKTA---KRTHLDDIRH 293          

HSP 2 Score: 107.842 bits (268), Expect = 1.094e-24
Identity = 54/136 (39.71%), Postives = 80/136 (58.82%), Query Frame = 3
Query: 1116 KSIENLLNIDYGPSSSFYKLAEEDTCYELTDREYIYKLCPFKKTSQMSKDSGPETELGKWGHWDGEE-NQYSMQVYDNGLPCWNGPNRQTKVMIYCGVENKLTEASEPSRCEYLFKFETPSACDKSSEQLNDHDEL 1520
            +  E+  N DYG  S++  L  +D C++   ++Y Y+ CPF + +Q    +   T LG +  W G E N+YS   + +G  CWNGP R T + I CG EN+L E +EP++CEYLF F TP AC    +++  H+EL
Sbjct:  375 REAEDFANSDYGEDSAWAAL--KDKCFDRNVQQYTYQFCPFGQNTQKDTGAYSGTSLGSFKEWSGPEGNKYSKMHFGDGQQCWNGPKRSTDITIECGEENELVEVTEPAKCEYLFTFRTPLACADPDKEVV-HEEL 507          
BLAST of Glucosidase 2 subunit beta vs. Ensembl Celegans
Match: ZK1307.8 (pep chromosome:WBcel235:II:9624637:9626626:1 gene:WBGene00014249.1 transcript:ZK1307.8.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:ZK1307.8)

HSP 1 Score: 189.119 bits (479), Expect = 3.630e-53
Identity = 102/274 (37.23%), Postives = 163/274 (59.49%), Query Frame = 1
Query:  106 RGIPFSKLSFYQPNSP--FKCLDGSGVISWMKVNDDYCDCRDATDEPGTSACSNGVFHCDNKGHKPQIIPSSRVNDGICDCCDGQDEYSGIINCTNTCWELGAIAREEMKRVKEAAEKGHKIYQEYIKQYETSVSEKRSRLETINEQFKVLSEEKLVSERSKTDSENNESEAKRVFEENWKKLQSENETKLERYKISKAFNELDIDVNERVTFEELQSHHEFDIDQNGDVSPEEAKEYLSERESVNEIEFTEIVWPNIKQIFKFKERTENDLRH 921
            +G+P S+L+ YQP++   F+CLDGS  I + ++NDDYCDC+D +DEPGTSAC N  F+C N GHK   IP++RVND +CDCCDG DEY   ++C N C ELG  AR E ++V   A KG++  QE  K+ +     K   +E + ++  +L  +++  E  K  +E+ E + +      W+ +++E     ++ + ++ F+ELD+D N ++  EEL+ +   D D +G VS +E K Y +  E+  E  F  +++  +K     K    +D+RH
Sbjct:   28 KGVPHSRLNLYQPSTTDTFRCLDGSQTILYSQLNDDYCDCKDGSDEPGTSACGNAFFYCSNVGHKGNFIPTNRVNDKLCDCCDGSDEYDSGVDCPNICDELGRAARIEHEKVANIARKGYQKRQELAKEGQALRDSKLKDVEPLRQERALLEPDRVKFEGEKKVAEDAERKLQDEHRNQWESVKNEK----KKLRAAEWFDELDLDKNGKIDKEELRQNIFLDDDNDGFVSEDEVKAYHTVDEADLE-HFQSLMYDRLKTA---KRTHLDDIRH 293          

HSP 2 Score: 107.842 bits (268), Expect = 1.094e-24
Identity = 54/136 (39.71%), Postives = 80/136 (58.82%), Query Frame = 3
Query: 1116 KSIENLLNIDYGPSSSFYKLAEEDTCYELTDREYIYKLCPFKKTSQMSKDSGPETELGKWGHWDGEE-NQYSMQVYDNGLPCWNGPNRQTKVMIYCGVENKLTEASEPSRCEYLFKFETPSACDKSSEQLNDHDEL 1520
            +  E+  N DYG  S++  L  +D C++   ++Y Y+ CPF + +Q    +   T LG +  W G E N+YS   + +G  CWNGP R T + I CG EN+L E +EP++CEYLF F TP AC    +++  H+EL
Sbjct:  375 REAEDFANSDYGEDSAWAAL--KDKCFDRNVQQYTYQFCPFGQNTQKDTGAYSGTSLGSFKEWSGPEGNKYSKMHFGDGQQCWNGPKRSTDITIECGEENELVEVTEPAKCEYLFTFRTPLACADPDKEVV-HEEL 507          
BLAST of Glucosidase 2 subunit beta vs. Ensembl Celegans
Match: ZK1307.8 (pep chromosome:WBcel235:II:9624637:9626641:1 gene:WBGene00014249.1 transcript:ZK1307.8.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:ZK1307.8)

HSP 1 Score: 189.119 bits (479), Expect = 3.630e-53
Identity = 102/274 (37.23%), Postives = 163/274 (59.49%), Query Frame = 1
Query:  106 RGIPFSKLSFYQPNSP--FKCLDGSGVISWMKVNDDYCDCRDATDEPGTSACSNGVFHCDNKGHKPQIIPSSRVNDGICDCCDGQDEYSGIINCTNTCWELGAIAREEMKRVKEAAEKGHKIYQEYIKQYETSVSEKRSRLETINEQFKVLSEEKLVSERSKTDSENNESEAKRVFEENWKKLQSENETKLERYKISKAFNELDIDVNERVTFEELQSHHEFDIDQNGDVSPEEAKEYLSERESVNEIEFTEIVWPNIKQIFKFKERTENDLRH 921
            +G+P S+L+ YQP++   F+CLDGS  I + ++NDDYCDC+D +DEPGTSAC N  F+C N GHK   IP++RVND +CDCCDG DEY   ++C N C ELG  AR E ++V   A KG++  QE  K+ +     K   +E + ++  +L  +++  E  K  +E+ E + +      W+ +++E     ++ + ++ F+ELD+D N ++  EEL+ +   D D +G VS +E K Y +  E+  E  F  +++  +K     K    +D+RH
Sbjct:   28 KGVPHSRLNLYQPSTTDTFRCLDGSQTILYSQLNDDYCDCKDGSDEPGTSACGNAFFYCSNVGHKGNFIPTNRVNDKLCDCCDGSDEYDSGVDCPNICDELGRAARIEHEKVANIARKGYQKRQELAKEGQALRDSKLKDVEPLRQERALLEPDRVKFEGEKKVAEDAERKLQDEHRNQWESVKNEK----KKLRAAEWFDELDLDKNGKIDKEELRQNIFLDDDNDGFVSEDEVKAYHTVDEADLE-HFQSLMYDRLKTA---KRTHLDDIRH 293          

HSP 2 Score: 107.842 bits (268), Expect = 1.094e-24
Identity = 54/136 (39.71%), Postives = 80/136 (58.82%), Query Frame = 3
Query: 1116 KSIENLLNIDYGPSSSFYKLAEEDTCYELTDREYIYKLCPFKKTSQMSKDSGPETELGKWGHWDGEE-NQYSMQVYDNGLPCWNGPNRQTKVMIYCGVENKLTEASEPSRCEYLFKFETPSACDKSSEQLNDHDEL 1520
            +  E+  N DYG  S++  L  +D C++   ++Y Y+ CPF + +Q    +   T LG +  W G E N+YS   + +G  CWNGP R T + I CG EN+L E +EP++CEYLF F TP AC    +++  H+EL
Sbjct:  375 REAEDFANSDYGEDSAWAAL--KDKCFDRNVQQYTYQFCPFGQNTQKDTGAYSGTSLGSFKEWSGPEGNKYSKMHFGDGQQCWNGPKRSTDITIECGEENELVEVTEPAKCEYLFTFRTPLACADPDKEVV-HEEL 507          
BLAST of Glucosidase 2 subunit beta vs. Ensembl Fly
Match: GCS2beta (gene:FBgn0032643 transcript:FBtr0080998)

HSP 1 Score: 196.438 bits (498), Expect = 2.524e-86
Identity = 133/386 (34.46%), Postives = 191/386 (49.48%), Query Frame = 1
Query:   94 VDKPRGIPFSKLSFYQPNS---PFKCLDGSGVISWMKVNDDYCDCRDATDEPGTSACSNGVFHCDNKGHKPQIIPSSRVNDGICDCCDGQDEYSGIINCTNTCWELGAIAREEMKRVKEAAEKGHKIYQEYIKQYETSVSEKRSRLETINEQFKVLSEEKLVSERSKTDSENNESEAKRVFEENWKKL-----QSENETKLERYKISKAFNELDIDVNERVTFEELQSHHEFDIDQNGDVSPEEAKEYLSERESVNEIEFTEIVWPNIKQIFKFKE------------------------------------RTENDLRHDFEGAVPDPVDQAXXXXXXXXXXXXXXXAKMEYDDETNRLISIANDARNSYNELNEKFMKLENEKN 1119
            V +P G+P +K S YQP +    + CLDGS  I +  +NDDYCDC D +DEPGT+AC  G FHC NKGH+P  IPSS+V DGICDCCDG DE S  + C NTC ELGA A  + +   E  ++G +  QE I + +   +E+ +R   ++++ K     +   E+ K  +E  E+EA  +F+E  +++     Q+E E +  R + +  F   D + +  V   EL      D D+NG V+ EEAK +L ERE V+   F  + WP IK +    E                                      ++D+  D EG      D+ P+          EE     YD ET RLI  AN+ARN+  E+     +++ E N
Sbjct:   29 VPRPLGVPLAKASLYQPRAGENSWTCLDGSRTIPFSHINDDYCDCADGSDEPGTAACPQGQFHCVNKGHQPVNIPSSQVQDGICDCCDGSDE-SETVGCPNTCLELGAAAAVQRRNAAELHKRGAERRQEMITRGKQMRAEREARRLELDQRRKEQELLRAEKEQLKQTAEALEAEAIEIFKEQQREVDADTAQAEREPQQMRQEATLTFVRYDTNKDGFVEVTELMVDMNLDRDRNGVVTVEEAKYFLDERERVDLDAFVTLAWPRIKSLQMLAEGLFQPPQPEEVAQQPEVTTESIQPTPPKLSEEQAELAGDDDIEADEEGEEDQYDDEEPDVGVGEASPDAEEATPPNYDPETQRLIQQANEARNALEEVERSLREIQQEVN 413          

HSP 2 Score: 143.28 bits (360), Expect = 2.524e-86
Identity = 65/113 (57.52%), Postives = 75/113 (66.37%), Query Frame = 3
Query: 1191 CYELTDREYIYKLCPFKKTSQMSKDSGPETELGKWGHWDGEENQYSMQVYDNGLPCWNGPNRQTKVMIYCGVENKLTEASEPSRCEYLFKFETPSACDK---SSEQLNDHDEL 1520
            CY   DREY+Y LCPF + SQ S+  GPET LG+W  W GE  QYS Q Y NG  CWNGPNR   + I C +E K+T  SEP+RCEY F+FETP+ACD     SE  N HDEL
Sbjct:  436 CYNFEDREYVYTLCPFDRASQKSRSGGPETTLGRWDKWSGEPKQYSQQKYTNGAACWNGPNRSAIINISCALEPKITAVSEPNRCEYYFEFETPAACDSEAFQSESENLHDEL 548          
BLAST of Glucosidase 2 subunit beta vs. Ensembl Fly
Match: GCS2beta (gene:FBgn0032643 transcript:FBtr0305684)

HSP 1 Score: 196.438 bits (498), Expect = 2.524e-86
Identity = 133/386 (34.46%), Postives = 191/386 (49.48%), Query Frame = 1
Query:   94 VDKPRGIPFSKLSFYQPNS---PFKCLDGSGVISWMKVNDDYCDCRDATDEPGTSACSNGVFHCDNKGHKPQIIPSSRVNDGICDCCDGQDEYSGIINCTNTCWELGAIAREEMKRVKEAAEKGHKIYQEYIKQYETSVSEKRSRLETINEQFKVLSEEKLVSERSKTDSENNESEAKRVFEENWKKL-----QSENETKLERYKISKAFNELDIDVNERVTFEELQSHHEFDIDQNGDVSPEEAKEYLSERESVNEIEFTEIVWPNIKQIFKFKE------------------------------------RTENDLRHDFEGAVPDPVDQAXXXXXXXXXXXXXXXAKMEYDDETNRLISIANDARNSYNELNEKFMKLENEKN 1119
            V +P G+P +K S YQP +    + CLDGS  I +  +NDDYCDC D +DEPGT+AC  G FHC NKGH+P  IPSS+V DGICDCCDG DE S  + C NTC ELGA A  + +   E  ++G +  QE I + +   +E+ +R   ++++ K     +   E+ K  +E  E+EA  +F+E  +++     Q+E E +  R + +  F   D + +  V   EL      D D+NG V+ EEAK +L ERE V+   F  + WP IK +    E                                      ++D+  D EG      D+ P+          EE     YD ET RLI  AN+ARN+  E+     +++ E N
Sbjct:   29 VPRPLGVPLAKASLYQPRAGENSWTCLDGSRTIPFSHINDDYCDCADGSDEPGTAACPQGQFHCVNKGHQPVNIPSSQVQDGICDCCDGSDE-SETVGCPNTCLELGAAAAVQRRNAAELHKRGAERRQEMITRGKQMRAEREARRLELDQRRKEQELLRAEKEQLKQTAEALEAEAIEIFKEQQREVDADTAQAEREPQQMRQEATLTFVRYDTNKDGFVEVTELMVDMNLDRDRNGVVTVEEAKYFLDERERVDLDAFVTLAWPRIKSLQMLAEGLFQPPQPEEVAQQPEVTTESIQPTPPKLSEEQAELAGDDDIEADEEGEEDQYDDEEPDVGVGEASPDAEEATPPNYDPETQRLIQQANEARNALEEVERSLREIQQEVN 413          

HSP 2 Score: 143.28 bits (360), Expect = 2.524e-86
Identity = 65/113 (57.52%), Postives = 75/113 (66.37%), Query Frame = 3
Query: 1191 CYELTDREYIYKLCPFKKTSQMSKDSGPETELGKWGHWDGEENQYSMQVYDNGLPCWNGPNRQTKVMIYCGVENKLTEASEPSRCEYLFKFETPSACDK---SSEQLNDHDEL 1520
            CY   DREY+Y LCPF + SQ S+  GPET LG+W  W GE  QYS Q Y NG  CWNGPNR   + I C +E K+T  SEP+RCEY F+FETP+ACD     SE  N HDEL
Sbjct:  436 CYNFEDREYVYTLCPFDRASQKSRSGGPETTLGRWDKWSGEPKQYSQQKYTNGAACWNGPNRSAIINISCALEPKITAVSEPNRCEYYFEFETPAACDSEAFQSESENLHDEL 548          
BLAST of Glucosidase 2 subunit beta vs. Ensembl Fly
Match: CG7685 (gene:FBgn0038619 transcript:FBtr0083630)

HSP 1 Score: 85.8853 bits (211), Expect = 8.327e-19
Identity = 47/105 (44.76%), Postives = 61/105 (58.10%), Query Frame = 1
Query:  106 RGIPFSKLSFYQPN--SPFKCLDGSGVISWMKVNDDYCDCR-DATDEPGTSACSNGVFHCDNK-----GHKPQI-IPSSRVNDGICDCCDGQDEYSGIINCTNTC 393
            RG        Y+PN    F+CLDGS  I +  +ND+YCDC  D +DEP T+AC+ G F+C  +     G    I + SSR+ND +CDCCDG DE+S    C N C
Sbjct:  108 RGTRLFDYDAYKPNFEGKFRCLDGSKEIPFDHLNDNYCDCEEDGSDEPSTNACAKGRFYCRYQKRHITGRGLDIYVASSRINDHVCDCCDGSDEWSTATKCPNDC 212          
BLAST of Glucosidase 2 subunit beta vs. Ensembl Fly
Match: CG7685 (gene:FBgn0038619 transcript:FBtr0333830)

HSP 1 Score: 85.5001 bits (210), Expect = 9.852e-19
Identity = 47/105 (44.76%), Postives = 61/105 (58.10%), Query Frame = 1
Query:  106 RGIPFSKLSFYQPN--SPFKCLDGSGVISWMKVNDDYCDCR-DATDEPGTSACSNGVFHCDNK-----GHKPQI-IPSSRVNDGICDCCDGQDEYSGIINCTNTC 393
            RG        Y+PN    F+CLDGS  I +  +ND+YCDC  D +DEP T+AC+ G F+C  +     G    I + SSR+ND +CDCCDG DE+S    C N C
Sbjct:  102 RGTRLFDYDAYKPNFEGKFRCLDGSKEIPFDHLNDNYCDCEEDGSDEPSTNACAKGRFYCRYQKRHITGRGLDIYVASSRINDHVCDCCDGSDEWSTATKCPNDC 206          
BLAST of Glucosidase 2 subunit beta vs. Ensembl Zebrafish
Match: prkcsh (protein kinase C substrate 80K-H [Source:ZFIN;Acc:ZDB-GENE-040426-770])

HSP 1 Score: 223.402 bits (568), Expect = 1.422e-65
Identity = 118/262 (45.04%), Postives = 166/262 (63.36%), Query Frame = 1
Query:   94 VDKPRGIPFSKLSFYQPNSPFKCLDGSGVISWMKVNDDYCDCRDATDEPGTSACSNGVFHCDNKGHKPQIIPSSRVNDGICDCCDGQDEYSGIINCTNTCWELGAIAREEMKRVKEAAEKGHKIYQEYIKQYETSVSEKRSRLETINEQFKVLSEEKLVSERS-KTDSENNESEAKRVFEENWKKLQSENETKLERYKISKAFNELDIDVNERVTFEELQSHHEFDIDQNGDVSPEEAKEYLSERESVNEIEFTEIVWPNIK 876
            V +PRG+P SK  FY+ N PF CLDGS  I + +VNDDYCDC+D +DEPGT+AC NG FHC N G+KP  IPSSR+NDGICDCCD  DEY+    C NTC ELG   RE ++++ E  ++G  + Q+ I++ +    EK+S++  + +  K L EEK+ + R+ K  +E  E EAK    + W++ ++      ++ K+++AF ELD + +  V+  ELQSH E D D +G +S  EAK  +   E V+   F E VW NIK
Sbjct:   22 VHRPRGVPLSKKPFYEENKPFTCLDGSKTILFDQVNDDYCDCKDGSDEPGTAACPNGKFHCTNAGYKPTFIPSSRINDGICDCCDTTDEYNSGAKCENTCKELGRKEREVLQKMAEITKEGFLLKQQLIEEAKKGRGEKQSKVTEMQDNKKQL-EEKVEALRTVKETAEQPEREAKERHLKAWEEQKAAIRLVKDKAKMAEAFLELDDNADGLVSVSELQSHAELDSDTDGTLSEAEAKGLMGGVEQVDTATF-ESVWENIK 281          

HSP 2 Score: 131.339 bits (329), Expect = 3.223e-32
Identity = 64/127 (50.39%), Postives = 81/127 (63.78%), Query Frame = 3
Query: 1116 KSIENLLNIDYGPSSSFYKLAEEDTCYELTDREYIYKLCPFKKTSQMSKDSGPETELGKWGHWDG-EENQYSMQVYDNGLPCWNGPNRQTKVMIYCGVENKLTEASEPSRCEYLFKFETPSACDKSS 1493
            ++IE  L+ D+GP++ F  L  +  CYEL+  EYIY+LCPF + SQ  K  G ET LG WG W G E N+Y    Y++G  CW GPNR T V + CG E  LT  SEPSRCEYL +F TP+ C + S
Sbjct:  393 RNIEKELSFDFGPNAEFTYLYSQ--CYELSTSEYIYRLCPFNRVSQKPKFGGSETNLGTWGSWSGPENNKYLSMKYEHGTGCWQGPNRSTTVKLTCGKETMLTSTSEPSRCEYLMEFITPAVCQEPS 517          
BLAST of Glucosidase 2 subunit beta vs. Ensembl Zebrafish
Match: prkcsh (protein kinase C substrate 80K-H [Source:ZFIN;Acc:ZDB-GENE-040426-770])

HSP 1 Score: 223.402 bits (568), Expect = 1.422e-65
Identity = 118/262 (45.04%), Postives = 166/262 (63.36%), Query Frame = 1
Query:   94 VDKPRGIPFSKLSFYQPNSPFKCLDGSGVISWMKVNDDYCDCRDATDEPGTSACSNGVFHCDNKGHKPQIIPSSRVNDGICDCCDGQDEYSGIINCTNTCWELGAIAREEMKRVKEAAEKGHKIYQEYIKQYETSVSEKRSRLETINEQFKVLSEEKLVSERS-KTDSENNESEAKRVFEENWKKLQSENETKLERYKISKAFNELDIDVNERVTFEELQSHHEFDIDQNGDVSPEEAKEYLSERESVNEIEFTEIVWPNIK 876
            V +PRG+P SK  FY+ N PF CLDGS  I + +VNDDYCDC+D +DEPGT+AC NG FHC N G+KP  IPSSR+NDGICDCCD  DEY+    C NTC ELG   RE ++++ E  ++G  + Q+ I++ +    EK+S++  + +  K L EEK+ + R+ K  +E  E EAK    + W++ ++      ++ K+++AF ELD + +  V+  ELQSH E D D +G +S  EAK  +   E V+   F E VW NIK
Sbjct:   22 VHRPRGVPLSKKPFYEENKPFTCLDGSKTILFDQVNDDYCDCKDGSDEPGTAACPNGKFHCTNAGYKPTFIPSSRINDGICDCCDTTDEYNSGAKCENTCKELGRKEREVLQKMAEITKEGFLLKQQLIEEAKKGRGEKQSKVTEMQDNKKQL-EEKVEALRTVKETAEQPEREAKERHLKAWEEQKAAIRLVKDKAKMAEAFLELDDNADGLVSVSELQSHAELDSDTDGTLSEAEAKGLMGGVEQVDTATF-ESVWENIK 281          

HSP 2 Score: 131.339 bits (329), Expect = 3.223e-32
Identity = 64/127 (50.39%), Postives = 81/127 (63.78%), Query Frame = 3
Query: 1116 KSIENLLNIDYGPSSSFYKLAEEDTCYELTDREYIYKLCPFKKTSQMSKDSGPETELGKWGHWDG-EENQYSMQVYDNGLPCWNGPNRQTKVMIYCGVENKLTEASEPSRCEYLFKFETPSACDKSS 1493
            ++IE  L+ D+GP++ F  L  +  CYEL+  EYIY+LCPF + SQ  K  G ET LG WG W G E N+Y    Y++G  CW GPNR T V + CG E  LT  SEPSRCEYL +F TP+ C + S
Sbjct:  393 RNIEKELSFDFGPNAEFTYLYSQ--CYELSTSEYIYRLCPFNRVSQKPKFGGSETNLGTWGSWSGPENNKYLSMKYEHGTGCWQGPNRSTTVKLTCGKETMLTSTSEPSRCEYLMEFITPAVCQEPS 517          
BLAST of Glucosidase 2 subunit beta vs. Ensembl Zebrafish
Match: prkcsh (protein kinase C substrate 80K-H [Source:ZFIN;Acc:ZDB-GENE-040426-770])

HSP 1 Score: 223.402 bits (568), Expect = 1.422e-65
Identity = 118/262 (45.04%), Postives = 166/262 (63.36%), Query Frame = 1
Query:   94 VDKPRGIPFSKLSFYQPNSPFKCLDGSGVISWMKVNDDYCDCRDATDEPGTSACSNGVFHCDNKGHKPQIIPSSRVNDGICDCCDGQDEYSGIINCTNTCWELGAIAREEMKRVKEAAEKGHKIYQEYIKQYETSVSEKRSRLETINEQFKVLSEEKLVSERS-KTDSENNESEAKRVFEENWKKLQSENETKLERYKISKAFNELDIDVNERVTFEELQSHHEFDIDQNGDVSPEEAKEYLSERESVNEIEFTEIVWPNIK 876
            V +PRG+P SK  FY+ N PF CLDGS  I + +VNDDYCDC+D +DEPGT+AC NG FHC N G+KP  IPSSR+NDGICDCCD  DEY+    C NTC ELG   RE ++++ E  ++G  + Q+ I++ +    EK+S++  + +  K L EEK+ + R+ K  +E  E EAK    + W++ ++      ++ K+++AF ELD + +  V+  ELQSH E D D +G +S  EAK  +   E V+   F E VW NIK
Sbjct:   22 VHRPRGVPLSKKPFYEENKPFTCLDGSKTILFDQVNDDYCDCKDGSDEPGTAACPNGKFHCTNAGYKPTFIPSSRINDGICDCCDTTDEYNSGAKCENTCKELGRKEREVLQKMAEITKEGFLLKQQLIEEAKKGRGEKQSKVTEMQDNKKQL-EEKVEALRTVKETAEQPEREAKERHLKAWEEQKAAIRLVKDKAKMAEAFLELDDNADGLVSVSELQSHAELDSDTDGTLSEAEAKGLMGGVEQVDTATF-ESVWENIK 281          

HSP 2 Score: 131.339 bits (329), Expect = 3.223e-32
Identity = 64/127 (50.39%), Postives = 81/127 (63.78%), Query Frame = 3
Query: 1116 KSIENLLNIDYGPSSSFYKLAEEDTCYELTDREYIYKLCPFKKTSQMSKDSGPETELGKWGHWDG-EENQYSMQVYDNGLPCWNGPNRQTKVMIYCGVENKLTEASEPSRCEYLFKFETPSACDKSS 1493
            ++IE  L+ D+GP++ F  L  +  CYEL+  EYIY+LCPF + SQ  K  G ET LG WG W G E N+Y    Y++G  CW GPNR T V + CG E  LT  SEPSRCEYL +F TP+ C + S
Sbjct:  393 RNIEKELSFDFGPNAEFTYLYSQ--CYELSTSEYIYRLCPFNRVSQKPKFGGSETNLGTWGSWSGPENNKYLSMKYEHGTGCWQGPNRSTTVKLTCGKETMLTSTSEPSRCEYLMEFITPAVCQEPS 517          
BLAST of Glucosidase 2 subunit beta vs. Ensembl Zebrafish
Match: prkcsh (protein kinase C substrate 80K-H [Source:ZFIN;Acc:ZDB-GENE-040426-770])

HSP 1 Score: 190.274 bits (482), Expect = 2.193e-56
Identity = 95/207 (45.89%), Postives = 136/207 (65.70%), Query Frame = 1
Query:   94 VDKPRGIPFSKLSFYQPNSPFKCLDGSGVISWMKVNDDYCDCRDATDEPGTSACSNGVFHCDNKGHKPQIIPSSRVNDGICDCCDGQDEYSGIINCTNTCWELGAIAREEMKRVKEAAEKGHKIYQEYIKQYETSVSEKRSRLETINEQFKVLSEEKLVSERS-KTDSENNESEAKRVFEENWKKLQSENETKLERYKISKAFNELD 711
            V +PRG+P SK  FY+ N PF CLDGS  I + +VNDDYCDC+D +DEPGT+AC NG FHC N G+KP  IPSSR+NDGICDCCD  DEY+    C NTC ELG   RE ++++ E  ++G  + Q+ I++ +    EK+S++  + +  K L EEK+ + R+ K  +E  E EAK    + W++ ++      ++ K+++AF ELD
Sbjct:   22 VHRPRGVPLSKKPFYEENKPFTCLDGSKTILFDQVNDDYCDCKDGSDEPGTAACPNGKFHCTNAGYKPTFIPSSRINDGICDCCDTTDEYNSGAKCENTCKELGRKEREVLQKMAEITKEGFLLKQQLIEEAKKGRGEKQSKVTEMQDNKKQL-EEKVEALRTVKETAEQPEREAKERHLKAWEEQKAAIRLVKDKAKMAEAFLELD 227          
BLAST of Glucosidase 2 subunit beta vs. Ensembl Zebrafish
Match: prkcsh (protein kinase C substrate 80K-H [Source:ZFIN;Acc:ZDB-GENE-040426-770])

HSP 1 Score: 180.644 bits (457), Expect = 1.152e-52
Identity = 89/183 (48.63%), Postives = 121/183 (66.12%), Query Frame = 1
Query:   94 VDKPRGIPFSKLSFYQPNSPFKCLDGSGVISWMKVNDDYCDCRDATDEPGTSACSNGVFHCDNKGHKPQIIPSSRVNDGICDCCDGQDEYSGIINCTNTCWELGAIAREEMKRVKEAAEKGHKIYQEYIKQYETSVSEKRSRLETINEQFKVLSEEKLVSERS-KTDSENNESEAKRVFEENW 639
            V +PRG+P SK  FY+ N PF CLDGS  I + +VNDDYCDC+D +DEPGT+AC NG FHC N G+KP  IPSSR+NDGICDCCD  DEY+    C NTC ELG   RE ++++ E  ++G  + Q+ I++ +    EK+S++  + +  K L EEK+ + R+ K  +E  E EAK    + W
Sbjct:   65 VHRPRGVPLSKKPFYEENKPFTCLDGSKTILFDQVNDDYCDCKDGSDEPGTAACPNGKFHCTNAGYKPTFIPSSRINDGICDCCDTTDEYNSGAKCENTCKELGRKEREVLQKMAEITKEGFLLKQQLIEEAKKGRGEKQSKVTEMQDNKKQL-EEKVEALRTVKETAEQPEREAKERHLKAW 246          
BLAST of Glucosidase 2 subunit beta vs. Ensembl Xenopus
Match: prkcsh (protein kinase C substrate 80K-H [Source:NCBI gene;Acc:394983])

HSP 1 Score: 229.565 bits (584), Expect = 2.099e-94
Identity = 128/339 (37.76%), Postives = 185/339 (54.57%), Query Frame = 1
Query:   94 VDKPRGIPFSKLSFYQPNSPFKCLDGSGVISWMKVNDDYCDCRDATDEPGTSACSNGVFHCDNKGHKPQIIPSSRVNDGICDCCDGQDEYSGIINCTNTCWELGAIAREEMKRVKEAAEKGHKIYQEYIKQYETSVSEKRSRLETINEQFKVLSEEKLVSERSKTDSENNESEAKRVFEENWKKLQSENETKLERYKISKAFNELDIDVNERVTFEELQSHHEFDIDQNGDVSPEEAKEYLSERESVNEIEFTEIVWPNIKQIFKFKERTENDLRHDFEGAVPDPVDQAXXXXXXXXXXXXXXXAKMEYDDETNRLISIANDARNSYNELNEKFMKLEN 1110
            V +PRG+  S  +FY  + PF CLDGS  I + +VNDDYCDC D TDEPGTSACSNG FHC N G+KPQ IPSSR+NDGICDCCD  DEY+    C NTC E+G   REE++   E A +G ++ Q  I+       EK+++L+ + ++ + L  +       K ++E  E EAK   ++ W++ +   + + ++ +  + F ELD D +  +   EL+SH E D+D +G +S EEA+  L +  SV+   F E VWP I++  KFK         + E  +P                         YD+ T  LI  A  AR++Y E  +    +E+
Sbjct:   60 VKRPRGVSLSNRAFYDDSKPFTCLDGSRTIPFDRVNDDYCDCADGTDEPGTSACSNGRFHCTNAGYKPQYIPSSRINDGICDCCDTTDEYNSGALCENTCREMGKKEREELQMAAEIAREGFRVKQLLIEDARKGREEKQTKLQDMVQRRQALQSQVDALRLQKEEAEKPEQEAKDAHQKAWEEHKEAEKAEEDKQRSLEVFTELDQDSDGMLAASELRSHMELDVDGDGAMSDEEAQSLLGD-TSVDVGIFQESVWPQIRE--KFKSEVPPSKAQEAEPEMP------------------------PYDEVTQALIDAAQTARSTYEEAEKSLRDMED 371          

HSP 2 Score: 137.887 bits (346), Expect = 2.099e-94
Identity = 67/137 (48.91%), Postives = 90/137 (65.69%), Query Frame = 3
Query: 1116 KSIENLLNIDYGPSSSFYKLAEEDTCYELTDREYIYKLCPFKKTSQMSKDSGPETELGKWGHWDG-EENQYSMQVYDNGLPCWNGPNRQTKVMIYCGVENKLTEASEPSRCEYLFKFETPSACDKSSEQL-NDHDEL 1520
            K +E  +++D+GP+  F  L  E  CYEL+  EY+Y+LCPF + +Q  K  G ET LG WG W G E+N++S   +++G  CW GPNR T V + CG ++ LT  SEPSRCEYL +F TP+AC    E L +DHDEL
Sbjct:  374 KGLEKEISLDFGPTGEFSYLYGE--CYELSTSEYVYRLCPFNRVTQKPKHGGSETNLGSWGSWAGPEDNKFSFMKFEHGTSCWQGPNRSTLVKLSCGKDSILTSTSEPSRCEYLMEFFTPAACHPPPEVLPDDHDEL 508          
BLAST of Glucosidase 2 subunit beta vs. Ensembl Xenopus
Match: prkcsh (protein kinase C substrate 80K-H [Source:NCBI gene;Acc:394983])

HSP 1 Score: 224.172 bits (570), Expect = 5.489e-66
Identity = 114/265 (43.02%), Postives = 163/265 (61.51%), Query Frame = 1
Query:   94 VDKPRGIPFSKLSFYQPNSPFKCLDGSGVISWMKVNDDYCDCRDATDEPGTSACSNGVFHCDNKGHKPQIIPSSRVNDGICDCCDGQDEYSGIINCTNTCWELGAIAREEMKRVKEAAEKGHKIYQEYIKQYETSVSEKRSRLETINEQFKVLSEEKLVSERSKTDSENNESEAKRVFEENWKKLQSENETKLERYKISKAFNELDIDVNERVTFEELQSHHEFDIDQNGDVSPEEAKEYLSERESVNEIEFTEIVWPNIKQIFK 888
            V +PRG+  S  +FY  + PF CLDGS  I + +VNDDYCDC D TDEPGTSACSNG FHC N G+KPQ IPSSR+NDGICDCCD  DEY+    C NTC E+G   REE++   E A +G ++ Q  I+       EK+++L+ + ++ + L  +       K ++E  E EAK   ++ W++ +   + + ++ +  + F ELD D +  +   EL+SH E D+D +G +S EEA+  L +  SV+   F E VWP I++ FK
Sbjct:   20 VKRPRGVSLSNRAFYDDSKPFTCLDGSRTIPFDRVNDDYCDCADGTDEPGTSACSNGRFHCTNAGYKPQYIPSSRINDGICDCCDTTDEYNSGALCENTCREMGKKEREELQMAAEIAREGFRVKQLLIEDARKGREEKQTKLQDMVQRRQALQSQVDALRLQKEEAEKPEQEAKDAHQKAWEEHKEAEKAEEDKQRSLEVFTELDQDSDGMLAASELRSHMELDVDGDGAMSDEEAQSLLGDT-SVDVGIFQESVWPQIREKFK 283          

HSP 2 Score: 137.117 bits (344), Expect = 3.730e-34
Identity = 66/137 (48.18%), Postives = 90/137 (65.69%), Query Frame = 3
Query: 1116 KSIENLLNIDYGPSSSFYKLAEEDTCYELTDREYIYKLCPFKKTSQMSKDSGPETELGKWGHWDG-EENQYSMQVYDNGLPCWNGPNRQTKVMIYCGVENKLTEASEPSRCEYLFKFETPSACDKSSEQL-NDHDEL 1520
            + +E  +++D+GP+  F  L  E  CYEL+  EY+Y+LCPF + +Q  K  G ET LG WG W G E+N++S   +++G  CW GPNR T V + CG ++ LT  SEPSRCEYL +F TP+AC    E L +DHDEL
Sbjct:  368 RGLEKEISLDFGPTGEFSYLYGE--CYELSTSEYVYRLCPFNRVTQKPKHGGSETNLGSWGSWAGPEDNKFSFMKFEHGTSCWQGPNRSTLVKLSCGKDSILTSTSEPSRCEYLMEFFTPAACHPPPEVLPDDHDEL 502          
BLAST of Glucosidase 2 subunit beta vs. Ensembl Xenopus
Match: prkcsh (protein kinase C substrate 80K-H [Source:NCBI gene;Acc:394983])

HSP 1 Score: 225.328 bits (573), Expect = 6.174e-66
Identity = 114/265 (43.02%), Postives = 163/265 (61.51%), Query Frame = 1
Query:   94 VDKPRGIPFSKLSFYQPNSPFKCLDGSGVISWMKVNDDYCDCRDATDEPGTSACSNGVFHCDNKGHKPQIIPSSRVNDGICDCCDGQDEYSGIINCTNTCWELGAIAREEMKRVKEAAEKGHKIYQEYIKQYETSVSEKRSRLETINEQFKVLSEEKLVSERSKTDSENNESEAKRVFEENWKKLQSENETKLERYKISKAFNELDIDVNERVTFEELQSHHEFDIDQNGDVSPEEAKEYLSERESVNEIEFTEIVWPNIKQIFK 888
            V +PRG+  S  +FY  + PF CLDGS  I + +VNDDYCDC D TDEPGTSACSNG FHC N G+KPQ IPSSR+NDGICDCCD  DEY+    C NTC E+G   REE++   E A +G ++ Q  I+       EK+++L+ + ++ + L  +       K ++E  E EAK   ++ W++ +   + + ++ +  + F ELD D +  +   EL+SH E D+D +G +S EEA+  L +  SV+   F E VWP I++ FK
Sbjct:   60 VKRPRGVSLSNRAFYDDSKPFTCLDGSRTIPFDRVNDDYCDCADGTDEPGTSACSNGRFHCTNAGYKPQYIPSSRINDGICDCCDTTDEYNSGALCENTCREMGKKEREELQMAAEIAREGFRVKQLLIEDARKGREEKQTKLQDMVQRRQALQSQVDALRLQKEEAEKPEQEAKDAHQKAWEEHKEAEKAEEDKQRSLEVFTELDQDSDGMLAASELRSHMELDVDGDGAMSDEEAQSLLGD-TSVDVGIFQESVWPQIREKFK 323          

HSP 2 Score: 137.887 bits (346), Expect = 3.037e-34
Identity = 67/137 (48.91%), Postives = 90/137 (65.69%), Query Frame = 3
Query: 1116 KSIENLLNIDYGPSSSFYKLAEEDTCYELTDREYIYKLCPFKKTSQMSKDSGPETELGKWGHWDG-EENQYSMQVYDNGLPCWNGPNRQTKVMIYCGVENKLTEASEPSRCEYLFKFETPSACDKSSEQL-NDHDEL 1520
            K +E  +++D+GP+  F  L  E  CYEL+  EY+Y+LCPF + +Q  K  G ET LG WG W G E+N++S   +++G  CW GPNR T V + CG ++ LT  SEPSRCEYL +F TP+AC    E L +DHDEL
Sbjct:  418 KGLEKEISLDFGPTGEFSYLYGE--CYELSTSEYVYRLCPFNRVTQKPKHGGSETNLGSWGSWAGPEDNKFSFMKFEHGTSCWQGPNRSTLVKLSCGKDSILTSTSEPSRCEYLMEFFTPAACHPPPEVLPDDHDEL 552          
BLAST of Glucosidase 2 subunit beta vs. Ensembl Xenopus
Match: prkcsh (protein kinase C substrate 80K-H [Source:NCBI gene;Acc:394983])

HSP 1 Score: 222.631 bits (566), Expect = 3.358e-65
Identity = 119/273 (43.59%), Postives = 164/273 (60.07%), Query Frame = 1
Query:   94 VDKPRGIPFSKLSFYQPNSPFKCLDGSGVISWMKVNDDYCDCRDATDEPGTSACSNGVFHCDNKGHKPQIIPSSRVNDGICDCCDGQDEYSGIINCTNTCWELGAIAREEMKRVKEAAEKGHKIYQEYIKQYETSVSEKRSRLETINEQFKVLSEEKLVSERSKTDSENNESEAKRVFEENW-KKLQSENETKLERYKISKAFNELDIDVNERVTFEELQSHHEFDIDQNGDVSPEEAKEYLSERESVNEIEFTEIVWPNIKQIFKFKERTEN 909
            V +PRG+  S  +FY  + PF CLDGS  I + +VNDDYCDC D TDEPGTSACSNG FHC N G+KPQ IPSSR+NDGICDCCD  DEY+    C NTC E+G   REE++   E A +G ++ Q  I+       EK+++L+ + ++ + L  +       K ++E  E EAK   ++ W +K + + +  LE       F ELD D +  +   EL+SH E D+D +G +S EEA+  L +  SV+   F E VWP I++ FK + R  N
Sbjct:   20 VKRPRGVSLSNRAFYDDSKPFTCLDGSRTIPFDRVNDDYCDCADGTDEPGTSACSNGRFHCTNAGYKPQYIPSSRINDGICDCCDTTDEYNSGALCENTCREMGKKEREELQMAAEIAREGFRVKQLLIEDARKGREEKQTKLQDMVQRRQALQSQVDALRLQKEEAEKPEQEAKDAHQKAWEEKAEEDKQRSLE------VFTELDQDSDGMLAASELRSHMELDVDGDGAMSDEEAQSLLGDT-SVDVGIFQESVWPQIREKFKSEVRCRN 285          

HSP 2 Score: 137.117 bits (344), Expect = 4.607e-34
Identity = 66/137 (48.18%), Postives = 90/137 (65.69%), Query Frame = 3
Query: 1116 KSIENLLNIDYGPSSSFYKLAEEDTCYELTDREYIYKLCPFKKTSQMSKDSGPETELGKWGHWDG-EENQYSMQVYDNGLPCWNGPNRQTKVMIYCGVENKLTEASEPSRCEYLFKFETPSACDKSSEQL-NDHDEL 1520
            + +E  +++D+GP+  F  L  E  CYEL+  EY+Y+LCPF + +Q  K  G ET LG WG W G E+N++S   +++G  CW GPNR T V + CG ++ LT  SEPSRCEYL +F TP+AC    E L +DHDEL
Sbjct:  386 RGLEKEISLDFGPTGEFSYLYGE--CYELSTSEYVYRLCPFNRVTQKPKHGGSETNLGSWGSWAGPEDNKFSFMKFEHGTSCWQGPNRSTLVKLSCGKDSILTSTSEPSRCEYLMEFFTPAACHPPPEVLPDDHDEL 520          
BLAST of Glucosidase 2 subunit beta vs. Ensembl Xenopus
Match: prkcsh (protein kinase C substrate 80K-H [Source:NCBI gene;Acc:394983])

HSP 1 Score: 214.542 bits (545), Expect = 5.573e-63
Identity = 114/270 (42.22%), Postives = 161/270 (59.63%), Query Frame = 1
Query:  112 IPFSKLSFYQPNSPFKCLDGSGVISWMKVNDDYCDCRDATDEPGTSACSNGVFHCDNKGHKPQIIPSSRVNDGICDCCDGQDEYSGIINCTNTCWELGAIAREEMKRVKEAAEKGHKIYQEYIKQYETSVSEKRSRLETINEQFKVLSEEKLVSERSKTDSENNESEAKRVFEENWKKLQSENETKLERYKISKAFNELDIDVNERVTFEELQSHHEFDIDQNGDVSPEEAKEYLSERESVNEIEFTEIVWPNIKQIFKFKERTENDLRH 921
             PF+  +FY  + PF CLDGS  I + +VNDDYCDC D TDEPGTSACSNG FHC N G+KPQ IPSSR+NDGICDCCD  DEY+    C NTC E+G   REE++   E A +G ++ Q  I+       EK+++L+ + ++ + L  +       K ++E  E EAK   ++ W++         ++ +  + F ELD D +  +   EL+SH E D+D +G +S EEA+  L +  SV+   F E VWP I++ FK + R  N  R+
Sbjct:   18 FPFADRAFYDDSKPFTCLDGSRTIPFDRVNDDYCDCADGTDEPGTSACSNGRFHCTNAGYKPQYIPSSRINDGICDCCDTTDEYNSGALCENTCREMGKKEREELQMAAEIAREGFRVKQLLIEDARKGREEKQTKLQDMVQRRQALQSQVDALRLQKEEAEKPEQEAKDAHQKAWEE---------DKQRSLEVFTELDQDSDGMLAASELRSHMELDVDGDGAMSDEEAQSLLGD-TSVDVGIFQESVWPQIREKFKSEVRCRNWPRY 277          

HSP 2 Score: 136.732 bits (343), Expect = 1.831e-34
Identity = 66/137 (48.18%), Postives = 90/137 (65.69%), Query Frame = 3
Query: 1116 KSIENLLNIDYGPSSSFYKLAEEDTCYELTDREYIYKLCPFKKTSQMSKDSGPETELGKWGHWDG-EENQYSMQVYDNGLPCWNGPNRQTKVMIYCGVENKLTEASEPSRCEYLFKFETPSACDKSSEQL-NDHDEL 1520
            + +E  +++D+GP+  F  L  E  CYEL+  EY+Y+LCPF + +Q  K  G ET LG WG W G E+N++S   +++G  CW GPNR T V + CG ++ LT  SEPSRCEYL +F TP+AC    E L +DHDEL
Sbjct:  301 RGLEKEISLDFGPTGEFSYLYGE--CYELSTSEYVYRLCPFNRVTQKPKHGGSETNLGSWGSWAGPEDNKFSFMKFEHGTSCWQGPNRSTLVKLSCGKDSILTSTSEPSRCEYLMEFFTPAACHPPPEVLPDDHDEL 435          
BLAST of Glucosidase 2 subunit beta vs. Ensembl Mouse
Match: Prkcsh (protein kinase C substrate 80K-H [Source:MGI Symbol;Acc:MGI:107877])

HSP 1 Score: 218.009 bits (554), Expect = 1.232e-63
Identity = 106/265 (40.00%), Postives = 163/265 (61.51%), Query Frame = 1
Query:   94 VDKPRGIPFSKLSFYQPNSPFKCLDGSGVISWMKVNDDYCDCRDATDEPGTSACSNGVFHCDNKGHKPQIIPSSRVNDGICDCCDGQDEYSGIINCTNTCWELGAIAREEMKRVKEAAEKGHKIYQEYIKQYETSVSEKRSRLETINEQFKVLSEEKLVSERSKTDSENNESEAKRVFEENWKKLQSENETKLERYKISKAFNELDIDVNERVTFEELQSHHEFDIDQNGDVSPEEAKEYLSERESVNEIEFTEIVWPNIKQIFK 888
            V +PRG+  S   FY+ + PF CLDG+  I + +VNDDYCDC+D +DEPGT+AC NG FHC N G+KP  I SSRVNDG+CDCCDG DEY+    C NTC E G   +E ++++ E   +G ++ +  I++++T+  EK+S+L  +    K L ++      +K ++E  E EAK    + W++ Q+  + + E+ + + AF ELD +++  V+  ELQ+H E D D +G +S EEA+  LS     +   F + VW  I+  ++
Sbjct:   17 VKRPRGVSLSNHHFYEESKPFTCLDGTATIPFDQVNDDYCDCKDGSDEPGTAACPNGSFHCTNTGYKPLYILSSRVNDGVCDCCDGTDEYNSGTVCENTCREKGRKEKESLQQLAEVTREGFRLKKILIEEWKTAREEKQSKLLELQAGKKSLEDQVETLRAAKEEAERPEKEAKDQHRKLWEEQQAAAKARREQERAASAFQELDDNMDGMVSLAELQTHPELDTDGDGALSEEEAQALLSGDTQTDTTSFYDRVWAAIRDKYR 281          

HSP 2 Score: 120.168 bits (300), Expect = 1.898e-28
Identity = 64/139 (46.04%), Postives = 84/139 (60.43%), Query Frame = 3
Query: 1116 KSIENLLNIDYGPSSSFYKLAEEDTCYELTDREYIYKLCPFKKTSQMSKDSGPETELGKWGHWDG-EENQYSMQVYDNGLPCWNGPNRQTKVMIYCGVENKLTEASEPSRCEYLFKFETPSACDKSSEQL---NDHDEL 1520
            +S+E  ++ D+GPS  F  L  +  CYELT  EY+Y+LCPFK  SQ  K  G  T LG WG W G + +++S   Y+ G  CW GPNR T V + CG E  +T  +EPSRCEYL +  TP+AC +   +     DHDEL
Sbjct:  385 RSLEQEISFDFGPSGEFAYLYSQ--CYELTTNEYVYRLCPFKLVSQKPKHGGSPTSLGTWGSWAGPDHDKFSAMKYEQGTGCWQGPNRSTTVRLLCGKETVVTSTTEPSRCEYLMELMTPAACPEPPPEAPSDGDHDEL 521          
BLAST of Glucosidase 2 subunit beta vs. Ensembl Mouse
Match: Prkcsh (protein kinase C substrate 80K-H [Source:MGI Symbol;Acc:MGI:107877])

HSP 1 Score: 218.009 bits (554), Expect = 1.232e-63
Identity = 106/265 (40.00%), Postives = 163/265 (61.51%), Query Frame = 1
Query:   94 VDKPRGIPFSKLSFYQPNSPFKCLDGSGVISWMKVNDDYCDCRDATDEPGTSACSNGVFHCDNKGHKPQIIPSSRVNDGICDCCDGQDEYSGIINCTNTCWELGAIAREEMKRVKEAAEKGHKIYQEYIKQYETSVSEKRSRLETINEQFKVLSEEKLVSERSKTDSENNESEAKRVFEENWKKLQSENETKLERYKISKAFNELDIDVNERVTFEELQSHHEFDIDQNGDVSPEEAKEYLSERESVNEIEFTEIVWPNIKQIFK 888
            V +PRG+  S   FY+ + PF CLDG+  I + +VNDDYCDC+D +DEPGT+AC NG FHC N G+KP  I SSRVNDG+CDCCDG DEY+    C NTC E G   +E ++++ E   +G ++ +  I++++T+  EK+S+L  +    K L ++      +K ++E  E EAK    + W++ Q+  + + E+ + + AF ELD +++  V+  ELQ+H E D D +G +S EEA+  LS     +   F + VW  I+  ++
Sbjct:   17 VKRPRGVSLSNHHFYEESKPFTCLDGTATIPFDQVNDDYCDCKDGSDEPGTAACPNGSFHCTNTGYKPLYILSSRVNDGVCDCCDGTDEYNSGTVCENTCREKGRKEKESLQQLAEVTREGFRLKKILIEEWKTAREEKQSKLLELQAGKKSLEDQVETLRAAKEEAERPEKEAKDQHRKLWEEQQAAAKARREQERAASAFQELDDNMDGMVSLAELQTHPELDTDGDGALSEEEAQALLSGDTQTDTTSFYDRVWAAIRDKYR 281          

HSP 2 Score: 120.168 bits (300), Expect = 1.898e-28
Identity = 64/139 (46.04%), Postives = 84/139 (60.43%), Query Frame = 3
Query: 1116 KSIENLLNIDYGPSSSFYKLAEEDTCYELTDREYIYKLCPFKKTSQMSKDSGPETELGKWGHWDG-EENQYSMQVYDNGLPCWNGPNRQTKVMIYCGVENKLTEASEPSRCEYLFKFETPSACDKSSEQL---NDHDEL 1520
            +S+E  ++ D+GPS  F  L  +  CYELT  EY+Y+LCPFK  SQ  K  G  T LG WG W G + +++S   Y+ G  CW GPNR T V + CG E  +T  +EPSRCEYL +  TP+AC +   +     DHDEL
Sbjct:  385 RSLEQEISFDFGPSGEFAYLYSQ--CYELTTNEYVYRLCPFKLVSQKPKHGGSPTSLGTWGSWAGPDHDKFSAMKYEQGTGCWQGPNRSTTVRLLCGKETVVTSTTEPSRCEYLMELMTPAACPEPPPEAPSDGDHDEL 521          
BLAST of Glucosidase 2 subunit beta vs. Ensembl Mouse
Match: Prkcsh (protein kinase C substrate 80K-H [Source:MGI Symbol;Acc:MGI:107877])

HSP 1 Score: 218.394 bits (555), Expect = 1.282e-63
Identity = 106/265 (40.00%), Postives = 163/265 (61.51%), Query Frame = 1
Query:   94 VDKPRGIPFSKLSFYQPNSPFKCLDGSGVISWMKVNDDYCDCRDATDEPGTSACSNGVFHCDNKGHKPQIIPSSRVNDGICDCCDGQDEYSGIINCTNTCWELGAIAREEMKRVKEAAEKGHKIYQEYIKQYETSVSEKRSRLETINEQFKVLSEEKLVSERSKTDSENNESEAKRVFEENWKKLQSENETKLERYKISKAFNELDIDVNERVTFEELQSHHEFDIDQNGDVSPEEAKEYLSERESVNEIEFTEIVWPNIKQIFK 888
            V +PRG+  S   FY+ + PF CLDG+  I + +VNDDYCDC+D +DEPGT+AC NG FHC N G+KP  I SSRVNDG+CDCCDG DEY+    C NTC E G   +E ++++ E   +G ++ +  I++++T+  EK+S+L  +    K L ++      +K ++E  E EAK    + W++ Q+  + + E+ + + AF ELD +++  V+  ELQ+H E D D +G +S EEA+  LS     +   F + VW  I+  ++
Sbjct:   17 VKRPRGVSLSNHHFYEESKPFTCLDGTATIPFDQVNDDYCDCKDGSDEPGTAACPNGSFHCTNTGYKPLYILSSRVNDGVCDCCDGTDEYNSGTVCENTCREKGRKEKESLQQLAEVTREGFRLKKILIEEWKTAREEKQSKLLELQAGKKSLEDQVETLRAAKEEAERPEKEAKDQHRKLWEEQQAAAKARREQERAASAFQELDDNMDGMVSLAELQTHPELDTDGDGALSEEEAQALLSGDTQTDTTSFYDRVWAAIRDKYR 281          

HSP 2 Score: 120.168 bits (300), Expect = 2.026e-28
Identity = 64/139 (46.04%), Postives = 84/139 (60.43%), Query Frame = 3
Query: 1116 KSIENLLNIDYGPSSSFYKLAEEDTCYELTDREYIYKLCPFKKTSQMSKDSGPETELGKWGHWDG-EENQYSMQVYDNGLPCWNGPNRQTKVMIYCGVENKLTEASEPSRCEYLFKFETPSACDKSSEQL---NDHDEL 1520
            +S+E  ++ D+GPS  F  L  +  CYELT  EY+Y+LCPFK  SQ  K  G  T LG WG W G + +++S   Y+ G  CW GPNR T V + CG E  +T  +EPSRCEYL +  TP+AC +   +     DHDEL
Sbjct:  392 RSLEQEISFDFGPSGEFAYLYSQ--CYELTTNEYVYRLCPFKLVSQKPKHGGSPTSLGTWGSWAGPDHDKFSAMKYEQGTGCWQGPNRSTTVRLLCGKETVVTSTTEPSRCEYLMELMTPAACPEPPPEAPSDGDHDEL 528          
BLAST of Glucosidase 2 subunit beta vs. Ensembl Mouse
Match: Prkcsh (protein kinase C substrate 80K-H [Source:MGI Symbol;Acc:MGI:107877])

HSP 1 Score: 135.961 bits (341), Expect = 1.418e-37
Identity = 61/102 (59.80%), Postives = 74/102 (72.55%), Query Frame = 1
Query:   94 VDKPRGIPFSKLSFYQPNSPFKCLDGSGVISWMKVNDDYCDCRDATDEPGTSACSNGVFHCDNKGHKPQIIPSSRVNDGICDCCDGQDEYSGIINCTNTCWE 399
            V +PRG+  S   FY+ + PF CLDG+  I + +VNDDYCDC+D +DEPGT+AC NG FHC N G+KP  I SSRVNDG+CDCCDG DEY+    C NTC E
Sbjct:   17 VKRPRGVSLSNHHFYEESKPFTCLDGTATIPFDQVNDDYCDCKDGSDEPGTAACPNGSFHCTNTGYKPLYILSSRVNDGVCDCCDGTDEYNSGTVCENTCRE 118          
BLAST of Glucosidase 2 subunit beta vs. Ensembl Mouse
Match: Gnptg (N-acetylglucosamine-1-phosphotransferase, gamma subunit [Source:MGI Symbol;Acc:MGI:2147006])

HSP 1 Score: 63.5438 bits (153), Expect = 1.654e-10
Identity = 37/113 (32.74%), Postives = 54/113 (47.79%), Query Frame = 3
Query: 1164 FYKLAEEDTCYELTDREYIYKLCPFKKTSQMSKD---SGPETELGKWGHWDGEENQYSMQVYDNGLPCWNGPNRQTKVMIYCGVENKLTEASEPSRCEYLFKFETPSACDKSS 1493
             ++LA +  C+ L +  Y Y+ CPF   +Q  +    +     LG W  W+   N +      +G  C +  +RQ+KV + CG  N+L   SEPS C Y   FETP  C   S
Sbjct:   64 LFRLAGK--CFSLVESTYKYEFCPFHNVTQHEQTFRWNAYSGILGIWHEWEIINNTFKGMWMTDGDSC-HSRSRQSKVELTCGKINRLAHVSEPSTCVYALTFETPLVCHPHS 173          
BLAST of Glucosidase 2 subunit beta vs. UniProt/SwissProt
Match: sp|O08795|GLU2B_MOUSE (Glucosidase 2 subunit beta OS=Mus musculus OX=10090 GN=Prkcsh PE=1 SV=1)

HSP 1 Score: 218.009 bits (554), Expect = 8.618e-63
Identity = 106/265 (40.00%), Postives = 163/265 (61.51%), Query Frame = 1
Query:   94 VDKPRGIPFSKLSFYQPNSPFKCLDGSGVISWMKVNDDYCDCRDATDEPGTSACSNGVFHCDNKGHKPQIIPSSRVNDGICDCCDGQDEYSGIINCTNTCWELGAIAREEMKRVKEAAEKGHKIYQEYIKQYETSVSEKRSRLETINEQFKVLSEEKLVSERSKTDSENNESEAKRVFEENWKKLQSENETKLERYKISKAFNELDIDVNERVTFEELQSHHEFDIDQNGDVSPEEAKEYLSERESVNEIEFTEIVWPNIKQIFK 888
            V +PRG+  S   FY+ + PF CLDG+  I + +VNDDYCDC+D +DEPGT+AC NG FHC N G+KP  I SSRVNDG+CDCCDG DEY+    C NTC E G   +E ++++ E   +G ++ +  I++++T+  EK+S+L  +    K L ++      +K ++E  E EAK    + W++ Q+  + + E+ + + AF ELD +++  V+  ELQ+H E D D +G +S EEA+  LS     +   F + VW  I+  ++
Sbjct:   17 VKRPRGVSLSNHHFYEESKPFTCLDGTATIPFDQVNDDYCDCKDGSDEPGTAACPNGSFHCTNTGYKPLYILSSRVNDGVCDCCDGTDEYNSGTVCENTCREKGRKEKESLQQLAEVTREGFRLKKILIEEWKTAREEKQSKLLELQAGKKSLEDQVETLRAAKEEAERPEKEAKDQHRKLWEEQQAAAKARREQERAASAFQELDDNMDGMVSLAELQTHPELDTDGDGALSEEEAQALLSGDTQTDTTSFYDRVWAAIRDKYR 281          

HSP 2 Score: 120.168 bits (300), Expect = 1.328e-27
Identity = 64/139 (46.04%), Postives = 84/139 (60.43%), Query Frame = 3
Query: 1116 KSIENLLNIDYGPSSSFYKLAEEDTCYELTDREYIYKLCPFKKTSQMSKDSGPETELGKWGHWDG-EENQYSMQVYDNGLPCWNGPNRQTKVMIYCGVENKLTEASEPSRCEYLFKFETPSACDKSSEQL---NDHDEL 1520
            +S+E  ++ D+GPS  F  L  +  CYELT  EY+Y+LCPFK  SQ  K  G  T LG WG W G + +++S   Y+ G  CW GPNR T V + CG E  +T  +EPSRCEYL +  TP+AC +   +     DHDEL
Sbjct:  385 RSLEQEISFDFGPSGEFAYLYSQ--CYELTTNEYVYRLCPFKLVSQKPKHGGSPTSLGTWGSWAGPDHDKFSAMKYEQGTGCWQGPNRSTTVRLLCGKETVVTSTTEPSRCEYLMELMTPAACPEPPPEAPSDGDHDEL 521          
BLAST of Glucosidase 2 subunit beta vs. UniProt/SwissProt
Match: sp|P14314|GLU2B_HUMAN (Glucosidase 2 subunit beta OS=Homo sapiens OX=9606 GN=PRKCSH PE=1 SV=2)

HSP 1 Score: 211.46 bits (537), Expect = 3.575e-60
Identity = 104/265 (39.25%), Postives = 159/265 (60.00%), Query Frame = 1
Query:   94 VDKPRGIPFSKLSFYQPNSPFKCLDGSGVISWMKVNDDYCDCRDATDEPGTSACSNGVFHCDNKGHKPQIIPSSRVNDGICDCCDGQDEYSGIINCTNTCWELGAIAREEMKRVKEAAEKGHKIYQEYIKQYETSVSEKRSRLETINEQFKVLSEEKLVSERSKTDSENNESEAKRVFEENWKKLQSENETKLERYKISKAFNELDIDVNERVTFEELQSHHEFDIDQNGDVSPEEAKEYLSERESVNEIEFTEIVWPNIKQIFK 888
            V +PRG+  +   FY  + PF CLDGS  I + +VNDDYCDC+D +DEPGT+AC NG FHC N G+KP  IPS+RVNDG+CDCCDG DEY+  + C NTC E G   RE ++++ E   +G ++ +  I+ ++ +  EK+ +L  +    K L ++  +    K ++E  E EAK   ++ W++  +  + + E+   + AF ELD D++  V+  ELQ+H E D D +G +S  EA+  LS     +   F + VW  I+  ++
Sbjct:   17 VKRPRGVSLTNHHFYDESKPFTCLDGSATIPFDQVNDDYCDCKDGSDEPGTAACPNGSFHCTNTGYKPLYIPSNRVNDGVCDCCDGTDEYNSGVICENTCKEKGRKERESLQQMAEVTREGFRLKKILIEDWKKAREEKQKKLIELQAGKKSLEDQVEMLRTVKEEAEKPEREAKEQHQKLWEEQLAAAKAQQEQELAADAFKELDDDMDGTVSVTELQTHPELDTDGDGALSEAEAQALLSGDTQTDATSFYDRVWAAIRDKYR 281          

HSP 2 Score: 114.775 bits (286), Expect = 9.698e-26
Identity = 58/139 (41.73%), Postives = 79/139 (56.83%), Query Frame = 3
Query: 1116 KSIENLLNIDYGPSSSFYKLAEEDTCYELTDREYIYKLCPFKKTSQMSKDSGPETELGKWGHWDG-EENQYSMQVYDNGLPCWNGPNRQTKVMIYCGVENKLTEASEPSRCEYLFKF---ETPSACDKSSEQLNDHDEL 1520
            +++E  ++ D+GP+  F  L  +  CYELT  EY+Y+LCPFK  SQ  K  G  T LG WG W G + +++S   Y+ G  CW GPNR T V + CG E  +T  +EPSRCEYL +             +   +DHDEL
Sbjct:  392 RNLEQEISFDFGPNGEFAYLYSQ--CYELTTNEYVYRLCPFKLVSQKPKLGGSPTSLGTWGSWIGPDHDKFSAMKYEQGTGCWQGPNRSTTVRLLCGKETMVTSTTEPSRCEYLMELMTPAACPEPPPEAPTEDDHDEL 528          
BLAST of Glucosidase 2 subunit beta vs. UniProt/SwissProt
Match: sp|Q28034|GLU2B_BOVIN (Glucosidase 2 subunit beta OS=Bos taurus OX=9913 GN=PRKCSH PE=2 SV=1)

HSP 1 Score: 188.734 bits (478), Expect = 8.579e-52
Identity = 103/265 (38.87%), Postives = 154/265 (58.11%), Query Frame = 1
Query:   94 VDKPRGIPFSKLSFYQPNSPFKCLDGSGVISWMKVNDDYCDCRDATDEPGTSACSNGVFHCDNKGHKPQIIPSSRVNDGICDCCDGQDEYSGIINCTNTCWELGAIAREEMKRVKEAAEKGHKIYQEYIKQYETSVSEKRSRLETINEQFKVLSEEKLVSERSKTDSENNESEAKRVFEENWKKLQSENETKLERYKISKAFNELDIDVNERVTFEELQSHHEFDIDQNGDVSPEEAKEYLSERESVNEIEFTEIVWPNIKQIFK 888
            V +PRG+  +   FY  + PF CLDGS  I + +VNDDYCDC+D +DEPGT+AC NG FHC N G+K   I S  VNDG+CDCCDG DEY+  I C NTC E G   RE ++++ E   +G ++ +  I+ ++ +  EK+ +L  +    K L ++  V    K ++E  E  AK      W++ Q+ ++ + ER   + AF ELD D++  V+  ELQ+H E D D +G +S  EA+  L     ++   F + VW  I+  ++
Sbjct:   16 VRRPRGVSLTNHHFYDESKPFTCLDGSASIPFDQVNDDYCDCKDGSDEPGTAACPNGSFHCTNTGYKALYISSRWVNDGVCDCCDGTDEYNSGIVCENTCKEKGRKERETLQQMAEVTREGFRLKKILIEDWKKAREEKQKKLIELQAGKKSLEDQVEVLRTLKEEAEKPEEAAKDQHRRLWEEQQAISKEQRERELAASAFQELDDDMDGAVSVAELQTHPELDTDGDGALSEGEAQTLLGGDAQMDAAFFYDRVWAAIRDKYR 280          

HSP 2 Score: 115.931 bits (289), Expect = 3.853e-26
Identity = 59/139 (42.45%), Postives = 78/139 (56.12%), Query Frame = 3
Query: 1116 KSIENLLNIDYGPSSSFYKLAEEDTCYELTDREYIYKLCPFKKTSQMSKDSGPETELGKWGHWDG-EENQYSMQVYDNGLPCWNGPNRQTKVMIYCGVENKLTEASEPSRCEYLFKFET---PSACDKSSEQLNDHDEL 1520
            +++E  ++ D+GP+  F  L  +  CYELT  EY+Y+LCPFK  SQ  K  G  T LG WG W G + +++S   Y+ G  CW GPNR T V + CG E  +T  +EPSRCEYL +  T               DHDEL
Sbjct:  397 RNLEQEISFDFGPNGEFAYLYSQ--CYELTTNEYVYRLCPFKLVSQKPKLGGSPTSLGTWGSWAGPDHDKFSAMKYEQGTGCWQGPNRSTTVRLLCGKETVVTSTTEPSRCEYLMELMTPAACPEPPPEYPVEGDHDEL 533          
BLAST of Glucosidase 2 subunit beta vs. UniProt/SwissProt
Match: sp|Q9FM96|PSL4_ARATH (Glucosidase 2 subunit beta OS=Arabidopsis thaliana OX=3702 GN=PSL4 PE=2 SV=1)

HSP 1 Score: 154.836 bits (390), Expect = 5.357e-39
Identity = 72/155 (46.45%), Postives = 98/155 (63.23%), Query Frame = 1
Query:  103 PRGIPFSKLS-----FYQPNSPFKCLDGSGVISWMKVNDDYCDCRDATDEPGTSACSNGVFHCDNKGHKPQIIPSSRVNDGICDCCDGQDEYSGIINCTNTCWELGAIAREEMKRVKEAAEKGHKIYQEYIKQYETSVSEKRSRLETINEQFKVL 552
            P   PF  +S     +Y+ +S  KC DGS   +  ++NDD+CDC D TDEPGTSAC  G F+C N GH P I+ SSRVNDGICDCCDG DEY G ++C NTCWE G  ARE +K+  E   +G  I ++ I+Q +  + +  + L+ +  + K+L
Sbjct:   32 PPNDPFLGISPQDEKYYKSSSEIKCKDGSKKFTKAQLNDDFCDCSDGTDEPGTSACPTGKFYCRNAGHSPVILFSSRVNDGICDCCDGSDEYDGHVSCQNTCWEAGKAARENLKKKIETYNQGLVIRRQEIEQAKVGLEKDAAELKKLKSEQKIL 186          

HSP 2 Score: 95.9005 bits (237), Expect = 2.033e-19
Identity = 48/121 (39.67%), Postives = 68/121 (56.20%), Query Frame = 3
Query: 1119 SIENLLNIDYGPSSSFYKLAEEDTCYELTDREYIYKLCPFKKTSQMSKDSGPETELGKWGHWDGEENQYSMQVYDNGLPCWNGPNRQTKVMIYCGVENKLTEASEPSRCEYLFKFETPSAC 1481
            S+E  L  D+GP   FY       C+E    +Y YK+C +K+ +Q  ++   +T LG+W  +   EN Y    Y NG  CWNGP+R  KV + CG++N+L +  EPSRCEY     TP+ C
Sbjct:  507 SLEKKLKQDFGPEKEFYSF--HGRCFESKQGKYTYKVCAYKEATQ--EEGYSKTRLGEWDKF---ENSYQFMSYTNGEKCWNGPDRSLKVKLRCGLKNELMDVDEPSRCEYAAILSTPARC 620          
BLAST of Glucosidase 2 subunit beta vs. UniProt/SwissProt
Match: sp|A2WNF5|GLU2B_ORYSI (Glucosidase 2 subunit beta OS=Oryza sativa subsp. indica OX=39946 GN=OsI_01383 PE=3 SV=1)

HSP 1 Score: 143.665 bits (361), Expect = 3.281e-35
Identity = 66/148 (44.59%), Postives = 92/148 (62.16%), Query Frame = 1
Query:  109 GIPFSKLSFYQPNSPFKCLDGSGVISWMKVNDDYCDCRDATDEPGTSACSNGVFHCDNKGHKPQIIPSSRVNDGICDCCDGQDEYSGIINCTNTCWELGAIAREEMKRVKEAAEKGHKIYQEYIKQYETSVSEKRSRLETINEQFKVL 552
            GIP    ++++     +C DGSG  +  K+NDD+CDC D TDEPGTSAC  G F+C N GH P  I SSRVNDGICDCCDG DEY   + C NTCWE G  AR+++K+     + G  I  + I++ + + ++  + L  +  + K+L
Sbjct:   29 GIPPQDEAYFR-GGVIRCRDGSGRFARDKLNDDFCDCPDGTDEPGTSACPEGKFYCQNAGHSPITIFSSRVNDGICDCCDGSDEYDSNVTCKNTCWEAGKAARDKLKKKVATYKSGVVIRNQEIQKAKVAFAKDEAELAKLKGEEKIL 175          

HSP 2 Score: 104.375 bits (259), Expect = 3.589e-22
Identity = 56/145 (38.62%), Postives = 78/145 (53.79%), Query Frame = 3
Query: 1119 SIENLLNIDYGPSSSFYKLAEEDTCYELTDREYIYKLCPFKKTSQMSKDSGPETELGKWGHWDGEENQYSMQVYDNGLPCWNGPNRQTKVMIYCGVENKLTEASEPSRCEYLFKFETPSACD-----------KSSEQLNDHDEL 1520
            ++ + L  D+G    FY     D C+E  + +Y+YK+CPFKK SQ+   S   T LG+W  +   E  Y +  + NG  CWNGP+R  KV + CG+ N+L    EPSRCEY+    TP+ CD           ++S    DHDEL
Sbjct:  477 TLTDKLKHDFGKEKEFYYFY--DQCFESKEGKYVYKVCPFKKASQVEGHST--TSLGRWDKF---EESYRVMQFSNGDRCWNGPDRSLKVRLRCGLNNELNGVDEPSRCEYVAVLSTPALCDEQKLKELEQKLEASSNQRDHDEL 614          
BLAST of Glucosidase 2 subunit beta vs. TrEMBL
Match: A0A1S3JT06 (glucosidase 2 subunit beta isoform X3 OS=Lingula unguis OX=7574 GN=LOC106175847 PE=4 SV=1)

HSP 1 Score: 273.092 bits (697), Expect = 1.254e-106
Identity = 155/359 (43.18%), Postives = 213/359 (59.33%), Query Frame = 1
Query:   94 VDKPRGIPFSKLSFYQPNSPFKCLDGSGVISWMKVNDDYCDCRDATDEPGTSACSNGVFHCDNKGHKPQIIPSSRVNDGICDCCDGQDEYSGIINCTNTCWELGAIAREEMKRVKEAAEKGHKIYQEYIKQYETSVSEKRSRLETINEQFKVLSEEKLVSERSKTDSENNESEAKRVFEENWKKLQSENETKLERYKISKAFNELDIDVNERVTFEELQSHHEFDIDQNGDVSPEEAKEYLSERESVNEIEFTEIVWPNIKQIFKFKE-RTENDLRHDF--------EGA--------VPDPVDQAXXXXXXXXXXXXXXXAKMEYDDETNRLISIANDARNSYNELNEKFMKLENEKN 1119
            VD+PRG+  SK S Y+P   F C D S  I + ++NDDYCDC+D +DEPGTSAC NG FHC N G++P  +PSSRVNDGICDCCDG DEYSG   C NTC E+G   REE +R+K+  ++G KI  +YIKQ + + +EKR+RLE + ++      +K   E  K ++E  E EAK   ++ W++ +S  E + E  K+ +AF ELD + +  +T  EL +H EFD + +G VS +E +EYL +  SV+   F E VWPNIK I KF E   E   + +         EGA         P P D+  EEE+  +          +YD+ET  LI  A++AR+ +    +KF  LENE N
Sbjct:   40 VDRPRGVSISKSSLYKPGDSFTCFDNSDTIPYSQINDDYCDCKDGSDEPGTSACPNGSFHCTNAGYRPLTMPSSRVNDGICDCCDGTDEYSGETTCVNTCREMGRAMREEQERMKQLLQEGVKIRDDYIKQGKDAKTEKRARLEALEKEKDEAEAKKNELENIKNEAEKPEKEAKDAHQKAWEEEKSLREAEKEAKKVEEAFAELDSNADGIITTSELATHPEFDKNSDGQVSEQEQQEYLEDHNSVDIKTFQEKVWPNIKDIIKFPEPEKEESAKGEATPPGEEMEEGAEFPEEAEVAPSPTDEKKEEEEEEKMP--------DYDEETKVLIQAADNARHEFTVAEQKFKDLENEMN 390          

HSP 2 Score: 144.821 bits (364), Expect = 1.254e-106
Identity = 68/135 (50.37%), Postives = 89/135 (65.93%), Query Frame = 3
Query: 1122 IENLLNIDYGPSSSFYKLAEEDTCYELTDREYIYKLCPFKKTSQMSKDSGPETELGKWGHWDG-EENQYSMQVYDNGLPCWNGPNRQTKVMIYCGVENKLTEASEPSRCEYLFKFETPSACDKSSEQLND-HDEL 1520
            + + L +D GP  +FY L  +  C+E TDREY+YKLCPF K SQ  K+ G ET LG WG W G EEN Y    Y+ G  CWNGP+R   + ++CG++N+L   +EP+RCEY F FETP+AC   ++Q  D HDEL
Sbjct:  392 LNHFLTLDLGPDDAFYPL--KGQCFEYTDREYVYKLCPFDKASQRPKNGGSETSLGTWGKWTGPEENIYLKMKYERGQGCWNGPDRSATITLHCGMKNELRNVAEPNRCEYTFDFETPAACTAPAKQSEDIHDEL 524          
BLAST of Glucosidase 2 subunit beta vs. TrEMBL
Match: A0A2T7NPL7 (Uncharacterized protein OS=Pomacea canaliculata OX=400727 GN=C0Q70_16355 PE=4 SV=1)

HSP 1 Score: 259.225 bits (661), Expect = 3.603e-103
Identity = 153/350 (43.71%), Postives = 210/350 (60.00%), Query Frame = 1
Query:   94 VDKPRGIPFSKLSFYQPNSPFKCLDGSGVISWMKVNDDYCDCRDATDEPGTSACSNGVFHCDNKGHKPQIIPSSRVNDGICDCCDGQDEYSG-----IINCTNTCWELGAIAREEMKRVKEAAEKGHKIYQEYIKQYETSVSEKRSRLETI-NEQFKVLSEEKLVSERSKTDSENNESEAKRVFEENWKKLQSENETKLERYKISKAFNELDIDVNERVTFEELQSHHEFDIDQNGDVSPEEAKEYLSERESVNEIEFTEIVWPNIKQIFK-FKERTENDLRHDFEGAVPDPVDQAXXXXXXXXXXXXXXXAKM-EYDDETNRLISIANDARNSYNELNEKFMKLENEKN 1119
            V++PRG+P SK SFYQ +  FKCL    VI +  +ND+YCDC D TDEPGTSAC+NG F+C+NKG + + I SSRVNDGICDCCDG DEY        I C NTC ELG   REE +  ++  E+G+KI QEYI+Q + +  EKR+RL  +  EQ K+++ +  +  + K   E  E EAK   E+ W++ +++ + + +R    KAF ELD++ +  V   E++ H EFDID +G VS EEA+EYLS+ E V+   F + +W  IK I+K   E  + D+    E    D         +  ++   EE  KM  YD+ET  LI+ A+ ARN YNE   K    ENE N
Sbjct:   18 VERPRGVPLSKASFYQVDQEFKCLTTGQVIPFEYINDNYCDCTDGTDEPGTSACANGFFYCENKGFRGEYITSSRVNDGICDCCDGSDEYGTEEFQENIKCINTCNELGRKEREEREAFQQLQEQGYKIKQEYIQQGKRTKDEKRARLSELEKEQVKLVAAKDELQAK-KEQVEAPEKEAKDRHEKAWEEFKAKKQAEKDRLAGIKAFAELDVNSDSAVDIVEMRRHSEFDIDSDGTVSEEEAREYLSDSEKVDLDTFLDRIWSTIKDIYKPPPEAADADMEAKGERESEDLSGSTASPPESKDKPKTEEELKMPPYDEETQNLIAAADAARNEYNEAENKVRDAENEIN 366          

HSP 2 Score: 147.517 bits (371), Expect = 3.603e-103
Identity = 74/137 (54.01%), Postives = 88/137 (64.23%), Query Frame = 3
Query: 1122 IENLLNIDYGPSSSFYKLAEEDTCYELTDREYIYKLCPFKKTSQMSKDSGPETELGKWGHWDG-EENQYSMQVYDNGLPCWNGPNRQTKVMIYCGVENKLTEASEPSRCEYLFKFETPSAC---DKSSEQLNDHDEL 1520
            ++  L +DYGP   F  L     C+E TDREY YKLCPF K  Q  K  G ET LGKWGHW G EE++Y    Y+NG  CWNGPNR   V ++CG ENKLT ASEPSRCEY F+F TP+ C   D +S   ++H EL
Sbjct:  368 LKKYLEVDYGPDEEFSSL--RGQCFEYTDREYTYKLCPFDKAVQKPKSGGSETSLGKWGHWYGPEEDKYVAMKYENGQNCWNGPNRSCHVNLHCGTENKLTGASEPSRCEYQFEFTTPARCQSPDSASPGSHEHTEL 502          
BLAST of Glucosidase 2 subunit beta vs. TrEMBL
Match: A0A1I8IR25 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 251.136 bits (640), Expect = 3.019e-98
Identity = 139/351 (39.60%), Postives = 197/351 (56.13%), Query Frame = 1
Query:  103 PRGIPFSKLSFYQPNSPFKCLDGSGVISWMKVNDDYCDCRDATDEPGTSACSNGVFHCDNKGHKPQIIPSSRVNDGICDCCDGQDEYSGIINCTNTCWELGAIAREEMKRVKEAAEKGHKIYQEYIKQYETSVSEKRSRLETINEQFKVLSEEKLVSERSKTDSENNESEAKRVFEENWKKLQSENETKLERYKISKAFNELDIDVNERVTFEELQSHHEFDIDQNGDVSPEEAKEYLSERESVNEIEFTEIVWPNIKQIFK-FKER----------TENDLRHDFEGAVPDPVDQAXXXXXXXXXXXXXXXAKM-EYDDETNRLISIANDARNSYNELNEKFMKLENEKN 1119
            PRG+P SKL  Y P  PF CLDGSG + W KVNDDYCDCRD +DEPGTSAC NG F C N+GHKP+ + +SRVNDG+CDCCDG DE+     C + CWE G  AREE +R      +GHK+Y  +++  E  ++ +R RL  I  + + +   K   E +K  +E+ E  AK   ++ W       E   ER +  + FNELD D + R+   +LQ H EFD++ +G V+ +E ++Y    +  +   F E +WPNIK IFK  K++           E     + + A+ +     P +    E+    E   M +Y  E   LI+ AN+AR +YN+ N++  KL  EK+
Sbjct:   34 PRGVPRSKLGLYDPTKPFNCLDGSGTLPWSKVNDDYCDCRDGSDEPGTSACKNGRFFCRNRGHKPKYLLASRVNDGVCDCCDGTDEFDSWTTCADVCWEEGKAAREEAERRHAQLVEGHKVYNTWLQDVEPKLAARRKRLAEIGAELERVQPSKAELESAKKAAEDAEEAAKTEHKKAW-------EESRERQRAERVFNELDSDSDGRIGLADLQIHPEFDVNADGGVTLDELRQYTEGLDEADFASFQEKIWPNIKTIFKSLKQQQQPAAESAPTAEESTDKEMDEALGEGAQPPPTQPTPQEDQAEAETDSMPDYPAEVQELINKANEAREAYNKANDEASKLTTEKS 377          

HSP 2 Score: 139.043 bits (349), Expect = 3.019e-98
Identity = 69/140 (49.29%), Postives = 93/140 (66.43%), Query Frame = 3
Query: 1113 KKSIENLLNIDYGPSSSFYKLAEEDTCYELTDREYIYKLCPFKKTSQMSKDSGPETELGKWGHWDGEE-NQYSMQVYDNGLPCWNGPNRQTKVMIYCGVENKLTEASEPSRCEYLFKFETPSACDK--SSEQLN-DHDEL 1520
            K  +E L+  DYGPS++F+ +A+   C E  D EY YKLCPFK++ Q+ K SG  T LG++  W G E ++YS Q Y +G  CWNGPNR TKV I CG + +L  ASEPSRCEY  +  TP+AC    ++E ++ +HDEL
Sbjct:  376 KSDLERLVERDYGPSNAFFPMADSGECAEFNDNEYTYKLCPFKESLQIQKASGSSTSLGRFKDWSGPEGDKYSSQEYTDGQGCWNGPNRSTKVRIVCGPKTELLSASEPSRCEYSMELATPAACHAHPTAETVDPEHDEL 515          
BLAST of Glucosidase 2 subunit beta vs. TrEMBL
Match: A0A1I8II80 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 251.136 bits (640), Expect = 3.449e-98
Identity = 139/351 (39.60%), Postives = 197/351 (56.13%), Query Frame = 1
Query:  103 PRGIPFSKLSFYQPNSPFKCLDGSGVISWMKVNDDYCDCRDATDEPGTSACSNGVFHCDNKGHKPQIIPSSRVNDGICDCCDGQDEYSGIINCTNTCWELGAIAREEMKRVKEAAEKGHKIYQEYIKQYETSVSEKRSRLETINEQFKVLSEEKLVSERSKTDSENNESEAKRVFEENWKKLQSENETKLERYKISKAFNELDIDVNERVTFEELQSHHEFDIDQNGDVSPEEAKEYLSERESVNEIEFTEIVWPNIKQIFK-FKER----------TENDLRHDFEGAVPDPVDQAXXXXXXXXXXXXXXXAKM-EYDDETNRLISIANDARNSYNELNEKFMKLENEKN 1119
            PRG+P SKL  Y P  PF CLDGSG + W KVNDDYCDCRD +DEPGTSAC NG F C N+GHKP+ + +SRVNDG+CDCCDG DE+     C + CWE G  AREE +R      +GHK+Y  +++  E  ++ +R RL  I  + + +   K   E +K  +E+ E  AK   ++ W       E   ER +  + FNELD D + R+   +LQ H EFD++ +G V+ +E ++Y    +  +   F E +WPNIK IFK  K++           E     + + A+ +     P +    E+    E   M +Y  E   LI+ AN+AR +YN+ N++  KL  EK+
Sbjct:   34 PRGVPRSKLGLYDPTKPFNCLDGSGTLPWSKVNDDYCDCRDGSDEPGTSACKNGRFFCRNRGHKPKYLLASRVNDGVCDCCDGTDEFDSWTTCADVCWEEGKAAREEAERRHALLVEGHKVYNTWLQDVEPKLAARRKRLAEIGAELERVQPSKAELESAKKAAEDAEEAAKTEHKKAW-------EESRERQRAERVFNELDSDSDGRIGLADLQIHPEFDVNADGGVTLDELRQYTEGLDEADFASFQEKIWPNIKTIFKSLKQQQQPAAESAPTAEESTDKEMDEALGEGAQPPPTQPTPQEDQAEAETDSMPDYPAEVQELINKANEAREAYNKANDEASKLTTEKS 377          

HSP 2 Score: 139.043 bits (349), Expect = 3.449e-98
Identity = 69/140 (49.29%), Postives = 93/140 (66.43%), Query Frame = 3
Query: 1113 KKSIENLLNIDYGPSSSFYKLAEEDTCYELTDREYIYKLCPFKKTSQMSKDSGPETELGKWGHWDGEE-NQYSMQVYDNGLPCWNGPNRQTKVMIYCGVENKLTEASEPSRCEYLFKFETPSACDK--SSEQLN-DHDEL 1520
            K  +E L+  DYGPS++F+ +A+   C E  D EY YKLCPFK++ Q+ K SG  T LG++  W G E ++YS Q Y +G  CWNGPNR TKV I CG + +L  ASEPSRCEY  +  TP+AC    ++E ++ +HDEL
Sbjct:  376 KSDLERLVERDYGPSNAFFPMADSGECAEFNDNEYTYKLCPFKESLQIQKASGSSTSLGRFKDWSGPEGDKYSSQEYTDGQGCWNGPNRSTKVRIVCGPKTELLSASEPSRCEYSMELATPAACHAHPTAETVDPEHDEL 515          
BLAST of Glucosidase 2 subunit beta vs. TrEMBL
Match: A0A267E0T6 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig002876g3 PE=4 SV=1)

HSP 1 Score: 250.751 bits (639), Expect = 3.779e-98
Identity = 139/351 (39.60%), Postives = 197/351 (56.13%), Query Frame = 1
Query:  103 PRGIPFSKLSFYQPNSPFKCLDGSGVISWMKVNDDYCDCRDATDEPGTSACSNGVFHCDNKGHKPQIIPSSRVNDGICDCCDGQDEYSGIINCTNTCWELGAIAREEMKRVKEAAEKGHKIYQEYIKQYETSVSEKRSRLETINEQFKVLSEEKLVSERSKTDSENNESEAKRVFEENWKKLQSENETKLERYKISKAFNELDIDVNERVTFEELQSHHEFDIDQNGDVSPEEAKEYLSERESVNEIEFTEIVWPNIKQIFK-FKER----------TENDLRHDFEGAVPDPVDQAXXXXXXXXXXXXXXXAKM-EYDDETNRLISIANDARNSYNELNEKFMKLENEKN 1119
            PRG+P SKL  Y P  PF CLDGSG + W KVNDDYCDCRD +DEPGTSAC NG F C N+GHKP+ + +SRVNDG+CDCCDG DE+     C + CWE G  AREE +R      +GHK+Y  +++  E  ++ +R RL  I  + + +   K   E +K  +E+ E  AK   ++ W       E   ER +  + FNELD D + R+   +LQ H EFD++ +G V+ +E ++Y    +  +   F E +WPNIK IFK  K++           E     + + A+ +     P +    E+    E   M +Y  E   LI+ AN+AR +YN+ N++  KL  EK+
Sbjct:   29 PRGVPRSKLGLYDPTKPFNCLDGSGTLPWSKVNDDYCDCRDGSDEPGTSACKNGRFFCRNRGHKPKYLLASRVNDGVCDCCDGTDEFDSWTTCADVCWEEGKAAREEAERRHAQLVEGHKVYNTWLQDVEPKLAARRKRLAEIGAELERVQPSKAELESAKKAAEDAEEAAKTEHKKAW-------EESRERQRAERVFNELDSDSDGRIGLADLQIHPEFDVNADGGVTLDELRQYTEGLDEADFASFQEKIWPNIKTIFKSLKQQQQPAAESAPTAEESTDKEMDEALGEGAQPPPTQPTPQEDQAEAETDSMPDYPAEVQELINKANEAREAYNKANDEASKLTTEKS 372          

HSP 2 Score: 139.043 bits (349), Expect = 3.779e-98
Identity = 69/140 (49.29%), Postives = 93/140 (66.43%), Query Frame = 3
Query: 1113 KKSIENLLNIDYGPSSSFYKLAEEDTCYELTDREYIYKLCPFKKTSQMSKDSGPETELGKWGHWDGEE-NQYSMQVYDNGLPCWNGPNRQTKVMIYCGVENKLTEASEPSRCEYLFKFETPSACDK--SSEQLN-DHDEL 1520
            K  +E L+  DYGPS++F+ +A+   C E  D EY YKLCPFK++ Q+ K SG  T LG++  W G E ++YS Q Y +G  CWNGPNR TKV I CG + +L  ASEPSRCEY  +  TP+AC    ++E ++ +HDEL
Sbjct:  371 KSDLERLVERDYGPSNAFFPMADSGECAEFNDNEYTYKLCPFKESLQIQKASGSSTSLGRFKDWSGPEGDKYSSQEYTDGQGCWNGPNRSTKVRIVCGPKTELLSASEPSRCEYSMELATPAACHAHPTAETVDPEHDEL 510          
BLAST of Glucosidase 2 subunit beta vs. Ensembl Cavefish
Match: prkcsh (protein kinase C substrate 80K-H [Source:ZFIN;Acc:ZDB-GENE-040426-770])

HSP 1 Score: 218.394 bits (555), Expect = 9.683e-64
Identity = 114/263 (43.35%), Postives = 166/263 (63.12%), Query Frame = 1
Query:   94 VDKPRGIPFSKLSFYQPNSPFKCLDGSGVISWMKVNDDYCDCRDATDEPGTSACSNGVFHCDNKGHKPQIIPSSRVNDGICDCCDGQDEYSGIINCTNTCWELGAIAREEMKRVKEAAEKGHKIYQEYIKQYETSVSEKRSRLETINEQFKVLSEEKLVSERS--KTDSENNESEAKRVFEENWKKLQSENETKLERYKISKAFNELDIDVNERVTFEELQSHHEFDIDQNGDVSPEEAKEYLSERESVNEIEFTEIVWPNIK 876
            V +PRG+P SK  FY+ + PF CLDGS  I + +VNDDYCDC+D +DEPGT+AC N  FHC N G++P  IPSSR+NDGICDCCD  DEY+    C NTC +LG   +E ++++ E  ++G  + Q+ I++ +    EK+++L  +    K L EEK+ + R+  K  +E  E EAK    + W++ ++  + + ++ K+++AF ELD D +  V+  ELQSH E D D +G VS  EA+  L   + V+   F E VW NIK
Sbjct:   23 VLRPRGVPLSKRQFYEEDKPFTCLDGSRTIPFDRVNDDYCDCKDGSDEPGTAACPNASFHCTNSGYRPTFIPSSRINDGICDCCDTTDEYNSGAKCENTCRDLGRKEQEVLQKMAEITKEGFLLKQQLIQEGKNGQQEKQNKLNELQNSKKEL-EEKVEALRTTVKETAEQPEKEAKERHLKAWEEQKAAVQAEKDKNKMAEAFLELDDDADGLVSVVELQSHMELDSDADGSVSEAEAQALLGGVDRVDTAAF-ESVWSNIK 283          

HSP 2 Score: 132.494 bits (332), Expect = 1.221e-32
Identity = 66/138 (47.83%), Postives = 86/138 (62.32%), Query Frame = 3
Query: 1116 KSIENLLNIDYGPSSSFYKLAEEDTCYELTDREYIYKLCPFKKTSQMSKDSGPETELGKWGHWDG-EENQYSMQVYDNGLPCWNGPNRQTKVMIYCGVENKLTEASEPSRCEYLFKFETPSACDK--SSEQLNDHDEL 1520
            ++IE  ++ D+GP   F  L  +  CYELT  EYIY+LCPF + SQ  K  G ET LG WG W G E N+Y +  Y++G  CW GPNR T V + CG E  +T  SEPSRCEYL +F TP+ C +  S++   +HDE 
Sbjct:  395 RNIEKEISFDFGPEGEFSYLYSQ--CYELTTSEYIYRLCPFNRVSQKPKYGGSETNLGTWGTWAGPESNKYLVMKYEHGTGCWQGPNRSTTVKLTCGKETVVTSTSEPSRCEYLMEFITPAVCQEPSSTDPQRNHDEF 530          
BLAST of Glucosidase 2 subunit beta vs. Ensembl Cavefish
Match: gnptg (N-acetylglucosamine-1-phosphate transferase subunit gamma [Source:NCBI gene;Acc:103034496])

HSP 1 Score: 71.2478 bits (173), Expect = 3.141e-13
Identity = 39/104 (37.50%), Postives = 50/104 (48.08%), Query Frame = 3
Query: 1191 CYELTDREYIYKLCPFKKTSQMSKD---SGPETELGKWGHWDGEENQYSMQVYDNGLPCWNGPNRQTKVMIYCGVENKLTEASEPSRCEYLFKFETPSACDKSS 1493
            C+   +  Y Y+ CPF   +Q  +    +     LG W  W+   N +S+     G  C N  NRQTKV+  CG  +KL   SEP  C Y F FETP  C K S
Sbjct:   69 CFSYIESTYKYEFCPFHNVTQHEQSFRWNAYSGILGIWHEWEIHNNTFSVMWMREGDSCGNK-NRQTKVLFTCGNSSKLARVSEPQTCVYSFTFETPLVCHKHS 171          
BLAST of Glucosidase 2 subunit beta vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000315.1 (pep scaffold:Pmarinus_7.0:GL477236:114578:118932:1 gene:ENSPMAG00000000282.1 transcript:ENSPMAT00000000315.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 146.362 bits (368), Expect = 1.357e-41
Identity = 71/133 (53.38%), Postives = 88/133 (66.17%), Query Frame = 1
Query:  121 SKLSFYQPNSPFKCLDGSGVISWMKVNDDYCDCRDATDEPGTSACSNGVFHCDNKGHKPQIIPSSRVNDGICDCCDGQDEYSGIINCTNTCWELGAIAREEMKRVKEAAEKGHKIYQEYIKQYETSVSEKRSR 519
            S+   Y     F CLDGS  + + +VNDDYCDC+D +DEPGTSAC NG FHC N G++PQ IPSSRVNDGICDCCD  DEY+    C NTC ELG   RE M R  E A +G +I Q+ +++ +    EK+ R
Sbjct:   32 SQRPLYDETKDFTCLDGSKTMPFDRVNDDYCDCKDGSDEPGTSACPNGNFHCTNAGYRPQNIPSSRVNDGICDCCDTTDEYNSGAVCENTCKELGQKDRENMARQAEVAAEGFRIKQQLMEETKRGKEEKQVR 164          
BLAST of Glucosidase 2 subunit beta vs. Ensembl Sea Lamprey
Match: ENSPMAT00000004224.1 (pep scaffold:Pmarinus_7.0:GL479914:16712:28221:-1 gene:ENSPMAG00000003850.1 transcript:ENSPMAT00000004224.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 61.6178 bits (148), Expect = 3.925e-11
Identity = 36/127 (28.35%), Postives = 72/127 (56.69%), Query Frame = 1
Query:  511 RSRLETINEQFKVLSEEKLVSERSKTDSENNESEAKRVFEENWKKLQSENETKLERYKISKAFNELDIDVNERVTFEELQSHHEFDIDQNGDVSPEEAKEYLSERESVNEIEFTEIVWPNIKQIFKF 891
            + +LE++ +  +   ++    ++ K   E+ E EAK   + +W++L+     ++E+ K   AF++LD + +  V+ +ELQ+H E D D++G+V+  E + +L   E+V+   F E VW  IK+  + 
Sbjct:    1 QKKLESLKDAKQAWEDKVHALQQQKETVESPEKEAKERHQASWEELKEARRKEVEKEKAGAAFSKLDENSDGFVSVQELQAHKELDADEDGEVTEVEVQTFLGGAETVDLQGFEESVWEGIKEKLRL 127          
BLAST of Glucosidase 2 subunit beta vs. Ensembl Sea Lamprey
Match: gnptg (N-acetylglucosamine-1-phosphate transferase subunit gamma [Source:ZFIN;Acc:ZDB-GENE-040625-18])

HSP 1 Score: 58.5362 bits (140), Expect = 3.229e-10
Identity = 35/97 (36.08%), Postives = 42/97 (43.30%), Query Frame = 3
Query: 1215 YIYKLCPFKKTSQMSKD---SGPETELGKWGHWDGEENQYSMQVYDNGLPCWNGPN-RQTKVMIYCGVENKLTEASEPSRCEYLFKFETPSACDKSS 1493
            Y Y LCPF   +Q  +    +     LG W  W+   N +   V  NG  C  G N RQ KV   CG +N L    EP  CEY   F +P  C   S
Sbjct:    1 YKYDLCPFHNVTQHEQSLRWNAYSGILGIWHEWEIVNNTFIAMVMRNGDNC--GSNSRQAKVTFRCGTQNALLGVEEPRTCEYAVNFTSPLVCHPHS 95          
BLAST of Glucosidase 2 subunit beta vs. Ensembl Yeast
Match: GTB1 (Glucosidase II beta subunit, forms a complex with alpha subunit Rot2p; involved in removal of two glucose residues from N-linked glycans during glycoprotein biogenesis in the ER; relocalizes from ER to cytoplasm upon DNA replication stress [Source:SGD;Acc:S000002629])

HSP 1 Score: 86.6557 bits (213), Expect = 2.460e-18
Identity = 62/212 (29.25%), Postives = 94/212 (44.34%), Query Frame = 1
Query:  109 GIPFSKLSFYQPNSP----FKCLDGSG-VISWMKVNDDYCDCRDATDEPGTSACS--------------NGVFHCDNKGHKPQIIPSSRVNDGICDCCDGQDE-------YSGIINCTNTCWELGAIAREEMKRVKEAAEKGHKIYQEY-----------------------IKQYETSVSEKRSRLETI-NEQFKVLSEEKLVSERSKTDS 594
            G+P+ K   Y  N P    + CL+    V+   ++ND  CDC D +DEPG++AC               N  F+CDNKG  P+ I  S V DGICDCCD  DE       +    NC+    E   +A +E+   +E  E   ++ ++Y                       IK     +SE R++LE +  E F  LS + ++ +  + +S
Sbjct:   30 GVPWEKQHLYDSNEPDLTKWHCLNHEDIVLDISQINDGVCDCPDGSDEPGSAACVEDIFKSVAEGGGKVNKYFYCDNKGFIPRYIRKSEVADGICDCCDCSDELLSGYELFDAGSNCSQLKNEFDIMASKELSSYREGKEALEELERKYGTKEEAITRGNCLEEDKEKASAEIKVLSNRLSENRAKLEQLRGEYFNQLSHDPILYQFEQLNS 241          

HSP 2 Score: 50.8322 bits (120), Expect = 4.218e-7
Identity = 20/53 (37.74%), Postives = 29/53 (54.72%), Query Frame = 3
Query: 1338 YDNGLPCWNGPNRQTKVMIYCGVENKLTEASEPSRCEYLFKFETPSACDKSSE 1496
            Y+NG  CWNGP R   V + C  + K+    E ++C Y+F    P  C+K+ E
Sbjct:  640 YENGDQCWNGPRRSATVFVRCSDKFKIRSVHEATKCNYIFDVVGPLGCNKTFE 692          
BLAST of Glucosidase 2 subunit beta vs. Ensembl Nematostella
Match: EDO36083 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SJQ2])

HSP 1 Score: 175.252 bits (443), Expect = 3.580e-48
Identity = 97/264 (36.74%), Postives = 144/264 (54.55%), Query Frame = 1
Query:  106 RGIPFSKLSFYQPNSPFKCLDGSGVISWMKVNDDYCDCRDATDEPGTSACSNGVFHCDNKGHKPQIIPSSRVNDGICDCCDGQDEYSGIINCTNTCWELGAIAREEMKRVKEAAEKGHKIYQEYIKQYETSVSEKRSRLETINEQFKVLSEEKLVSERSKTDSENNESEAKRVFEENWKKLQSENETKLERYKISKAFNELDIDVNERVTFEELQSHHEFDIDQNGDVSPEEAKEYLSERESVNEIEFTEIVWPNIKQIFKFKE 897
            RG+  +KL FY  +  F CLDGS  I +  VNDDYCDC D +DEPGT+AC NG FHC N G++P+  PSSRVNDGICDCCDG DEY G +NC +TC EL     E+ +   E A++G+   QE+ +      +E++ +LE +  Q +   +     + +K   E  E EAK   ++ W++ +++   +        AF  LD D +  +T  EL  +   D     + + EEA+E L   +  +   F + +W   K  +  K+
Sbjct:   30 RGVAITKLPFYDSSKDFTCLDGSLTIPFSSVNDDYCDCNDGSDEPGTAACPNGQFHCTNAGYRPKNYPSSRVNDGICDCCDGSDEYDGKVNCPDTCKELYKQEFEKRRHEIELAKQGYAKKQEFSQDGINKKTERKKKLEDLRLQLEQKKQVVAELQATKDKVEAVEKEAKDKHDKEWEETKAKILAERAHNAAVAAFKLLDTDNSGSLTSMELMVNPYMD----KEYTAEEARELLGGTDETDVDRFAKDLWTTFKDKYGVKQ 289          

HSP 2 Score: 137.887 bits (346), Expect = 5.956e-35
Identity = 65/133 (48.87%), Postives = 84/133 (63.16%), Query Frame = 3
Query: 1122 IENLLNIDYGPSSSFYKLAEEDTCYELTDREYIYKLCPFKKTSQMSKDSGPETELGKWGHWDGEENQYSMQVYDNGLPCWNGPNRQTKVMIYCGVENKLTEASEPSRCEYLFKFETPSACDKSSEQLNDHDEL 1520
            IE  LNID+G    F  L     C+E  DREY+YKLCPF + +Q  KD G  T +G WG W+G   +YS   Y +G  CWNGPNR T+V++ CG +N++T  SEPSRCEY  +F+TP+AC    + L  H EL
Sbjct:  404 IEKKLNIDFGEHEEFAALY--GNCFEFRDREYLYKLCPFDRATQEPKDGGASTSIGNWGEWNGSPYKYSRMKYSDGQNCWNGPNRSTQVILSCGPDNEVTSVSEPSRCEYQMEFKTPAACQHHEDIL--HQEL 532          
BLAST of Glucosidase 2 subunit beta vs. Ensembl Nematostella
Match: EDO38047 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SE51])

HSP 1 Score: 57.7658 bits (138), Expect = 4.469e-9
Identity = 34/116 (29.31%), Postives = 49/116 (42.24%), Query Frame = 3
Query: 1155 SSSFYKLAEEDTCYELTDREYIYKLCPFKKTSQMSKDS---GPETELGKWGHWDGEENQYSMQVYDNGLPCWNGPNRQTKVMIYCGVENKLTEASEPSRCEYLFKFETPSACDKSS 1493
            S   + ++    C+ LT+  Y Y+ CPF   +Q  + S        LG +  W  E N +   +   G  C N   R+T V + C     LT   EPS C Y     TP AC + +
Sbjct:   60 SGPLHLISLNGKCFSLTEYGYKYEFCPFHNVTQREQSSRWNAFHGILGVFQGWTIENNTFISMLMSEGDFCPNKMARRTDVFLKCASTPTLTSVKEPSTCHYELVLNTPLACPRDA 175          
BLAST of Glucosidase 2 subunit beta vs. Ensembl Medaka
Match: prkcsh (protein kinase C substrate 80K-H [Source:NCBI gene;Acc:101159748])

HSP 1 Score: 217.238 bits (552), Expect = 2.277e-63
Identity = 114/273 (41.76%), Postives = 165/273 (60.44%), Query Frame = 1
Query:   94 VDKPRGIPFSKLSFYQPNSPFKCLDGSGVISWMKVNDDYCDCRDATDEPGTSACSNGVFHCDNKGHKPQIIPSSRVNDGICDCCDGQDEYSGIINCTNTCWELGAIAREEMKRVKEAAEKGHKIYQEYIKQYETSVSEKRSRLETINEQFKVLSEEKLVSERS-KTDSENNESEAKRVFEENWKKLQSENETKLERYKISKAFNELDIDVNERVTFEELQSHHEFDIDQNGDVSPEEAKEYLSERESVNEIEFTEIVWPNIKQIFKFKERTEN 909
            V +PRG+P SK  FY+   PF CLDGS  + + +VNDDYCDC+D +DEPGT+AC NG FHC N G +P  IPSSR+NDGICDCCD  DEY+    C NTC ELG    E ++++ E A++G  + Q+ I + ++ + EK+++L  +    K L EEK+ + R+ K  +E  E+EAK    + W+  ++    + E+ ++S  F ELD D +  VT  EL  H E D D +G  +  +A+  L   + V+   F E VW NIK+ F  +  T+ 
Sbjct:   22 VQRPRGVPLSKRQFYEEGKPFTCLDGSLTVPFDRVNDDYCDCKDGSDEPGTAACPNGSFHCTNAGFRPAFIPSSRINDGICDCCDATDEYNSGATCQNTCKELGRKEIESLQKMAEIAKEGFLLKQQLIHEAKSGLEEKKTKLAEVQTNRKDL-EEKVDALRTVKEKAEQPENEAKERHLKAWEAQKAVVRMEKEKARMSGVFLELDKDGDGFVTVNELLPHSELDPDADGTFTETDAQGLLGGVDKVDAASF-ESVWNNIKEKFHSESHTDT 292          

HSP 2 Score: 127.102 bits (318), Expect = 8.343e-31
Identity = 61/123 (49.59%), Postives = 79/123 (64.23%), Query Frame = 3
Query: 1116 KSIENLLNIDYGPSSSFYKLAEEDTCYELTDREYIYKLCPFKKTSQMSKDSGPETELGKWGHWDG-EENQYSMQVYDNGLPCWNGPNRQTKVMIYCGVENKLTEASEPSRCEYLFKFETPSAC 1481
            +++E  ++ D+G S+ F  L  +  CYEL   EYIYKLCPF + SQ  K  G ET LG WG W G E++ YS+  Y++G  CW GPNR T V + CG E  +T  SEPSRCEYL +F TP+ C
Sbjct:  394 RNLEKEISFDFGASAEFAYLYSQ--CYELHTGEYIYKLCPFNRVSQKPKYGGSETNLGTWGKWAGPEDDVYSVMKYEHGTGCWQGPNRSTTVKLTCGKETVVTSTSEPSRCEYLMEFTTPAVC 514          
BLAST of Glucosidase 2 subunit beta vs. Ensembl Medaka
Match: ENSORLT00000005204.2 (glucosidase 2 subunit beta [Source:NCBI gene;Acc:101164594])

HSP 1 Score: 145.591 bits (366), Expect = 7.642e-38
Identity = 68/146 (46.58%), Postives = 92/146 (63.01%), Query Frame = 1
Query:  100 KPRGIPFSKLSFYQPNSPFKCLDGSGVISWMKVNDDYCDCRDATDEPGTSACSNGVFHCDNKGHKPQIIPSSRVNDGICDCCDGQDEYSGIINCTNTCWELGAIAREEMKRVKEAAEKGHKIYQEYIKQYETSVSEKRSRLETINE 537
            K RGI  S   FY+    F C+DGS +I + +VNDDYCDC D +DEPGTSAC +G F+C N G +P  IPSSRVNDGICDCCD  DEY+    C NTCW LG   R  ++      ++G ++ Q+ I++      EK+++L  + +
Sbjct:   25 KVRGISSSYKRFYREKKSFLCIDGSKLIPFEQVNDDYCDCADGSDEPGTSACPHGRFYCTNLGFRPHYIPSSRVNDGICDCCDASDEYNSHARCQNTCWNLGQRERAYVEGQMRTLDEGLRLKQQLIEEGVLLWREKQAQLRELQQ 170          

HSP 2 Score: 110.923 bits (276), Expect = 7.507e-26
Identity = 56/134 (41.79%), Postives = 76/134 (56.72%), Query Frame = 3
Query: 1122 IENLLNIDYGPSSSFYKLAEEDTCYELTDREYIYKLCPFKKTSQMSKDSGPETELGKWGHW-DGEENQYSMQVYDNGLPCWNGPNRQTKVMIYCGVENKLTEASEPSRCEYLFKFETPSACDKSSEQLNDHDEL 1520
            ++  L IDYG    F  L  +  CY+L   EY+Y LCPF   +Q S  +G E  LG WG W +  +N+Y+  +Y+NG PCW G +R T V + CG E  L    EPS+C+Y+  F+TP AC    +Q   H EL
Sbjct:  296 LQKKLAIDYGTDWEFLFLNGQ--CYKLKVYEYMYTLCPFNHITQKST-AGTEVSLGLWGMWAEPAKNRYNQMIYENGEPCWQGGSRSTTVTLTCGTETALRSVKEPSKCQYIMDFQTPVACQPVLKQRGVHSEL 426          
BLAST of Glucosidase 2 subunit beta vs. Ensembl Medaka
Match: ENSORLT00000045570.1 (glucosidase 2 subunit beta [Source:NCBI gene;Acc:101164594])

HSP 1 Score: 144.436 bits (363), Expect = 2.185e-37
Identity = 68/146 (46.58%), Postives = 92/146 (63.01%), Query Frame = 1
Query:  100 KPRGIPFSKLSFYQPNSPFKCLDGSGVISWMKVNDDYCDCRDATDEPGTSACSNGVFHCDNKGHKPQIIPSSRVNDGICDCCDGQDEYSGIINCTNTCWELGAIAREEMKRVKEAAEKGHKIYQEYIKQYETSVSEKRSRLETINE 537
            K RGI  S   FY+    F C+DGS +I + +VNDDYCDC D +DEPGTSAC +G F+C N G +P  IPSSRVNDGICDCCD  DEY+    C NTCW LG   R  ++      ++G ++ Q+ I++      EK+++L  + +
Sbjct:   27 KVRGISSSYKRFYREKKSFLCIDGSKLIPFEQVNDDYCDCADGSDEPGTSACPHGRFYCTNLGFRPHYIPSSRVNDGICDCCDASDEYNSHARCQNTCWNLGQRERAYVEGQMRTLDEGLRLKQQLIEEGVLLWREKQAQLRELQQ 172          

HSP 2 Score: 111.309 bits (277), Expect = 6.019e-26
Identity = 56/134 (41.79%), Postives = 76/134 (56.72%), Query Frame = 3
Query: 1122 IENLLNIDYGPSSSFYKLAEEDTCYELTDREYIYKLCPFKKTSQMSKDSGPETELGKWGHW-DGEENQYSMQVYDNGLPCWNGPNRQTKVMIYCGVENKLTEASEPSRCEYLFKFETPSACDKSSEQLNDHDEL 1520
            ++  L IDYG    F  L  +  CY+L   EY+Y LCPF   +Q S  +G E  LG WG W +  +N+Y+  +Y+NG PCW G +R T V + CG E  L    EPS+C+Y+  F+TP AC    +Q   H EL
Sbjct:  303 LQKKLAIDYGTDWEFLFLNGQ--CYKLKVYEYMYTLCPFNHITQKST-AGTEVSLGLWGMWAEPAKNRYNQMIYENGEPCWQGGSRSTTVTLTCGTETALRSVKEPSKCQYIMDFQTPVACQPVLKQRGVHSEL 433          
BLAST of Glucosidase 2 subunit beta vs. Ensembl Medaka
Match: gnptg (N-acetylglucosamine-1-phosphate transferase subunit gamma [Source:NCBI gene;Acc:101164438])

HSP 1 Score: 66.6254 bits (161), Expect = 1.220e-11
Identity = 36/104 (34.62%), Postives = 52/104 (50.00%), Query Frame = 3
Query: 1191 CYELTDREYIYKLCPFKKTSQMSKD---SGPETELGKWGHWDGEENQYSMQVYDNGLPCWNGPNRQTKVMIYCGVENKLTEASEPSRCEYLFKFETPSACDKSS 1493
            C+ L +  Y Y+ CPF   +Q  +    +     LG W  W+   N ++     +G  C    NR+TKV++ CG  +KL++ SEPS C Y   FETP  C   S
Sbjct:   63 CFSLIESMYKYEFCPFHNITQHEQSFRWNAYSGILGIWHEWEIVNNTFTGMWMRDGDTC-GTRNRETKVILVCGSSSKLSQVSEPSTCLYSITFETPLVCHPHS 165          
BLAST of Glucosidase 2 subunit beta vs. Ensembl Medaka
Match: gnptg (N-acetylglucosamine-1-phosphate transferase subunit gamma [Source:NCBI gene;Acc:101164438])

HSP 1 Score: 66.2402 bits (160), Expect = 1.528e-11
Identity = 36/104 (34.62%), Postives = 52/104 (50.00%), Query Frame = 3
Query: 1191 CYELTDREYIYKLCPFKKTSQMSKD---SGPETELGKWGHWDGEENQYSMQVYDNGLPCWNGPNRQTKVMIYCGVENKLTEASEPSRCEYLFKFETPSACDKSS 1493
            C+ L +  Y Y+ CPF   +Q  +    +     LG W  W+   N ++     +G  C    NR+TKV++ CG  +KL++ SEPS C Y   FETP  C   S
Sbjct:   72 CFSLIESMYKYEFCPFHNITQHEQSFRWNAYSGILGIWHEWEIVNNTFTGMWMRDGDTC-GTRNRETKVILVCGSSSKLSQVSEPSTCLYSITFETPLVCHPHS 174          
BLAST of Glucosidase 2 subunit beta vs. Planmine SMEST
Match: SMESG000051850.1 (SMESG000051850.1)

HSP 1 Score: 664.455 bits (1713), Expect = 0.000e+0
Identity = 341/341 (100.00%), Postives = 341/341 (100.00%), Query Frame = 1
Query:   94 VDKPRGIPFSKLSFYQPNSPFKCLDGSGVISWMKVNDDYCDCRDATDEPGTSACSNGVFHCDNKGHKPQIIPSSRVNDGICDCCDGQDEYSGIINCTNTCWELGAIAREEMKRVKEAAEKGHKIYQEYIKQYETSVSEKRSRLETINEQFKVLSEEKLVSERSKTDSENNESEAKRVFEENWKKLQSENETKLERYKISKAFNELDIDVNERVTFEELQSHHEFDIDQNGDVSPEEAKEYLSERESVNEIEFTEIVWPNIKQIFKFKERTENDLRHDFEGAVPDPVDQAXXXXXXXXXXXXXXXAKMEYDDETNRLISIANDARNSYNELNEKFMKLENEK 1116
            VDKPRGIPFSKLSFYQPNSPFKCLDGSGVISWMKVNDDYCDCRDATDEPGTSACSNGVFHCDNKGHKPQIIPSSRVNDGICDCCDGQDEYSGIINCTNTCWELGAIAREEMKRVKEAAEKGHKIYQEYIKQYETSVSEKRSRLETINEQFKVLSEEKLVSERSKTDSENNESEAKRVFEENWKKLQSENETKLERYKISKAFNELDIDVNERVTFEELQSHHEFDIDQNGDVSPEEAKEYLSERESVNEIEFTEIVWPNIKQIFKFKERTENDLRHDFEGAVPDPVDQAPEEEKLPEETPPEEPAKMEYDDETNRLISIANDARNSYNELNEKFMKLENEK
Sbjct:   18 VDKPRGIPFSKLSFYQPNSPFKCLDGSGVISWMKVNDDYCDCRDATDEPGTSACSNGVFHCDNKGHKPQIIPSSRVNDGICDCCDGQDEYSGIINCTNTCWELGAIAREEMKRVKEAAEKGHKIYQEYIKQYETSVSEKRSRLETINEQFKVLSEEKLVSERSKTDSENNESEAKRVFEENWKKLQSENETKLERYKISKAFNELDIDVNERVTFEELQSHHEFDIDQNGDVSPEEAKEYLSERESVNEIEFTEIVWPNIKQIFKFKERTENDLRHDFEGAVPDPVDQAPEEEKLPEETPPEEPAKMEYDDETNRLISIANDARNSYNELNEKFMKLENEK 358          

HSP 2 Score: 285.804 bits (730), Expect = 0.000e+0
Identity = 135/136 (99.26%), Postives = 135/136 (99.26%), Query Frame = 3
Query: 1113 KKSIENLLNIDYGPSSSFYKLAEEDTCYELTDREYIYKLCPFKKTSQMSKDSGPETELGKWGHWDGEENQYSMQVYDNGLPCWNGPNRQTKVMIYCGVENKLTEASEPSRCEYLFKFETPSACDKSSEQLNDHDEL 1520
            KKSIENLLNIDYGPSSSFYKLAEEDTCYELTDREYIYKLCPFKKTSQMSKDSGPETELGKWGHW GEENQYSMQVYDNGLPCWNGPNRQTKVMIYCGVENKLTEASEPSRCEYLFKFETPSACDKSSEQLNDHDEL
Sbjct:  358 KKSIENLLNIDYGPSSSFYKLAEEDTCYELTDREYIYKLCPFKKTSQMSKDSGPETELGKWGHWGGEENQYSMQVYDNGLPCWNGPNRQTKVMIYCGVENKLTEASEPSRCEYLFKFETPSACDKSSEQLNDHDEL 493          
BLAST of Glucosidase 2 subunit beta vs. Planmine SMEST
Match: SMESG000054962.1 (SMESG000054962.1)

HSP 1 Score: 76.2554 bits (186), Expect = 1.131e-15
Identity = 38/107 (35.51%), Postives = 51/107 (47.66%), Query Frame = 3
Query: 1191 CYELTDREYIYKLCPFKKTSQMSKDSG------PETELGKWGHWDGEENQYSMQVYDNGLPCWNGPNRQTKVMIYCGVENKLTEASEPSRCEYLFKFETPSACDKSS 1493
            CY+       Y+ CPF+  +Q+  ++           LG WG+W  E +++   VYD G  C N  NR T+V   CG E KL   SEP  CEY   F  P  CD  +
Sbjct:   27 CYKFETDTMSYEFCPFQNVTQIIINNNFGIFNTKRVLLGVWGYWIIENDKFVTAVYDVGETCNNIKNRSTRVRFICGTEYKLINVSEPEFCEYSAVFSLPLVCDNDT 133          
The following BLAST results are available for this feature:
BLAST of Glucosidase 2 subunit beta vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
PRKCSH6.297e-6139.25protein kinase C substrate 80K-H [Source:HGNC Symb... [more]
PRKCSH6.297e-6139.25protein kinase C substrate 80K-H [Source:HGNC Symb... [more]
PRKCSH7.444e-6139.25protein kinase C substrate 80K-H [Source:HGNC Symb... [more]
PRKCSH8.027e-6139.25protein kinase C substrate 80K-H [Source:HGNC Symb... [more]
PRKCSH2.216e-4548.95protein kinase C substrate 80K-H [Source:HGNC Symb... [more]
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BLAST of Glucosidase 2 subunit beta vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 4
Match NameE-valueIdentityDescription
ZK1307.83.630e-5337.23pep chromosome:WBcel235:II:9624590:9626625:1 gene:... [more]
ZK1307.83.630e-5337.23pep chromosome:WBcel235:II:9624613:9626625:1 gene:... [more]
ZK1307.83.630e-5337.23pep chromosome:WBcel235:II:9624637:9626626:1 gene:... [more]
ZK1307.83.630e-5337.23pep chromosome:WBcel235:II:9624637:9626641:1 gene:... [more]
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BLAST of Glucosidase 2 subunit beta vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 4
Match NameE-valueIdentityDescription
GCS2beta2.524e-8634.46gene:FBgn0032643 transcript:FBtr0080998[more]
GCS2beta2.524e-8634.46gene:FBgn0032643 transcript:FBtr0305684[more]
CG76858.327e-1944.76gene:FBgn0038619 transcript:FBtr0083630[more]
CG76859.852e-1944.76gene:FBgn0038619 transcript:FBtr0333830[more]
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BLAST of Glucosidase 2 subunit beta vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
prkcsh1.422e-6545.04protein kinase C substrate 80K-H [Source:ZFIN;Acc:... [more]
prkcsh1.422e-6545.04protein kinase C substrate 80K-H [Source:ZFIN;Acc:... [more]
prkcsh1.422e-6545.04protein kinase C substrate 80K-H [Source:ZFIN;Acc:... [more]
prkcsh2.193e-5645.89protein kinase C substrate 80K-H [Source:ZFIN;Acc:... [more]
prkcsh1.152e-5248.63protein kinase C substrate 80K-H [Source:ZFIN;Acc:... [more]
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BLAST of Glucosidase 2 subunit beta vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
prkcsh2.099e-9437.76protein kinase C substrate 80K-H [Source:NCBI gene... [more]
prkcsh5.489e-6643.02protein kinase C substrate 80K-H [Source:NCBI gene... [more]
prkcsh6.174e-6643.02protein kinase C substrate 80K-H [Source:NCBI gene... [more]
prkcsh3.358e-6543.59protein kinase C substrate 80K-H [Source:NCBI gene... [more]
prkcsh5.573e-6342.22protein kinase C substrate 80K-H [Source:NCBI gene... [more]
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BLAST of Glucosidase 2 subunit beta vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Prkcsh1.232e-6340.00protein kinase C substrate 80K-H [Source:MGI Symbo... [more]
Prkcsh1.232e-6340.00protein kinase C substrate 80K-H [Source:MGI Symbo... [more]
Prkcsh1.282e-6340.00protein kinase C substrate 80K-H [Source:MGI Symbo... [more]
Prkcsh1.418e-3759.80protein kinase C substrate 80K-H [Source:MGI Symbo... [more]
Gnptg1.654e-1032.74N-acetylglucosamine-1-phosphotransferase, gamma su... [more]
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BLAST of Glucosidase 2 subunit beta vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|O08795|GLU2B_MOUSE8.618e-6340.00Glucosidase 2 subunit beta OS=Mus musculus OX=1009... [more]
sp|P14314|GLU2B_HUMAN3.575e-6039.25Glucosidase 2 subunit beta OS=Homo sapiens OX=9606... [more]
sp|Q28034|GLU2B_BOVIN8.579e-5238.87Glucosidase 2 subunit beta OS=Bos taurus OX=9913 G... [more]
sp|Q9FM96|PSL4_ARATH5.357e-3946.45Glucosidase 2 subunit beta OS=Arabidopsis thaliana... [more]
sp|A2WNF5|GLU2B_ORYSI3.281e-3544.59Glucosidase 2 subunit beta OS=Oryza sativa subsp. ... [more]
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BLAST of Glucosidase 2 subunit beta vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1S3JT061.254e-10643.18glucosidase 2 subunit beta isoform X3 OS=Lingula u... [more]
A0A2T7NPL73.603e-10343.71Uncharacterized protein OS=Pomacea canaliculata OX... [more]
A0A1I8IR253.019e-9839.60Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8II803.449e-9839.60Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267E0T63.779e-9839.60Uncharacterized protein OS=Macrostomum lignano OX=... [more]
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BLAST of Glucosidase 2 subunit beta vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
prkcsh9.683e-6443.35protein kinase C substrate 80K-H [Source:ZFIN;Acc:... [more]
gnptg3.141e-1337.50N-acetylglucosamine-1-phosphate transferase subuni... [more]
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BLAST of Glucosidase 2 subunit beta vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 3
Match NameE-valueIdentityDescription
ENSPMAT00000000315.11.357e-4153.38pep scaffold:Pmarinus_7.0:GL477236:114578:118932:1... [more]
ENSPMAT00000004224.13.925e-1128.35pep scaffold:Pmarinus_7.0:GL479914:16712:28221:-1 ... [more]
gnptg3.229e-1036.08N-acetylglucosamine-1-phosphate transferase subuni... [more]
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BLAST of Glucosidase 2 subunit beta vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
GTB12.460e-1829.25Glucosidase II beta subunit, forms a complex with ... [more]
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BLAST of Glucosidase 2 subunit beta vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2
Match NameE-valueIdentityDescription
EDO360833.580e-4836.74Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO380474.469e-929.31Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Glucosidase 2 subunit beta vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
prkcsh2.277e-6341.76protein kinase C substrate 80K-H [Source:NCBI gene... [more]
ENSORLT00000005204.27.642e-3846.58glucosidase 2 subunit beta [Source:NCBI gene;Acc:1... [more]
ENSORLT00000045570.12.185e-3746.58glucosidase 2 subunit beta [Source:NCBI gene;Acc:1... [more]
gnptg1.220e-1134.62N-acetylglucosamine-1-phosphate transferase subuni... [more]
gnptg1.528e-1134.62N-acetylglucosamine-1-phosphate transferase subuni... [more]
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BLAST of Glucosidase 2 subunit beta vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000051850.10.000e+0100.00SMESG000051850.1[more]
SMESG000054962.11.131e-1535.51SMESG000054962.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30019477 ID=SMED30019477|Name=Glucosidase 2 subunit beta|organism=Schmidtea mediterranea sexual|type=transcript|length=1680bp
GTTTGTTTTAAATTTTACACATAATTAAGTAAGATTCAAAACATGTTGAA
ATTAATTATCATATGCATTTCTATATTAAATTCTATATCATGTGTAGACA
AACCTCGAGGAATACCGTTTTCAAAATTATCATTTTATCAACCTAATAGT
CCATTTAAGTGTCTTGATGGATCAGGAGTAATAAGTTGGATGAAAGTAAA
CGATGATTATTGTGATTGTCGTGATGCAACTGATGAACCTGGTACATCTG
CATGTTCTAATGGTGTTTTCCATTGCGATAACAAAGGTCACAAACCTCAA
ATTATTCCTTCATCTCGCGTTAATGATGGTATATGTGATTGTTGTGATGG
TCAAGATGAATACTCTGGAATAATAAATTGCACTAATACATGCTGGGAAT
TAGGTGCAATTGCTAGAGAAGAAATGAAACGTGTTAAAGAAGCTGCTGAA
AAAGGACACAAAATATATCAAGAATACATAAAGCAGTATGAAACATCGGT
ATCAGAAAAAAGATCTCGACTAGAAACAATTAATGAGCAATTTAAAGTTC
TCAGTGAAGAAAAACTTGTTTCAGAACGATCGAAAACAGATTCTGAAAAT
AATGAATCGGAAGCCAAACGGGTTTTTGAAGAAAATTGGAAAAAATTACA
ATCGGAAAATGAAACAAAATTGGAACGCTATAAAATATCTAAAGCTTTTA
ATGAATTGGATATTGATGTTAACGAAAGAGTAACATTTGAAGAATTGCAA
AGTCATCATGAATTCGATATCGATCAAAATGGAGATGTGTCACCAGAGGA
GGCAAAAGAGTATTTGAGTGAAAGGGAATCGGTAAATGAGATTGAATTCA
CGGAAATTGTCTGGCCGAATATCAAACAAATATTCAAATTCAAAGAAAGA
ACAGAAAACGATTTACGACATGATTTCGAGGGTGCAGTTCCTGATCCAGT
TGATCAAGCCCCAGAAGAAGAGAAATTGCCCGAGGAAACCCCACCCGAGG
AGCCAGCAAAAATGGAGTATGATGACGAGACAAATAGACTCATTTCTATT
GCCAATGATGCCCGGAACAGTTACAACGAATTGAATGAGAAATTTATGAA
GCTAGAGAACGAAAAAAATCTATAGAAAATCTTTTGAATATTGATTATGG
ACCGAGCTCATCTTTCTATAAGTTAGCCGAAGAAGACACGTGTTACGAAC
TAACTGACCGGGAATATATTTATAAATTATGCCCATTTAAAAAAACGAGC
CAAATGTCCAAAGATTCTGGTCCAGAAACCGAGCTCGGTAAATGGGGACA
TTGGGACGGTGAGGAGAACCAATATTCAATGCAGGTCTATGACAATGGGT
TACCGTGTTGGAATGGGCCAAACCGCCAGACAAAAGTAATGATTTACTGT
GGCGTAGAAAATAAGTTAACCGAGGCCTCCGAACCGAGCAGATGTGAATA
CCTTTTCAAATTTGAGACGCCTTCAGCTTGCGATAAGTCTTCTGAGCAGT
TGAATGATCACGATGAGTTGTGATTTACATTCTATTCAAACTAAGGTAAT
TCTCGTATTTCCTGCATGTTCGGTGCCGATTCTGTTGCTGATTCTCCCGA
TCAGGAGTGGCGCTGAACTCGCCTAATGTTGTTGGGCAACGTTGGAAATA
CACAAACAAATATATAGTAGCCTTTGTTGT
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protein sequence of SMED30019477-orf-1

>SMED30019477-orf-1 ID=SMED30019477-orf-1|Name=SMED30019477-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=361bp
MLKLIIICISILNSISCVDKPRGIPFSKLSFYQPNSPFKCLDGSGVISWM
KVNDDYCDCRDATDEPGTSACSNGVFHCDNKGHKPQIIPSSRVNDGICDC
CDGQDEYSGIINCTNTCWELGAIAREEMKRVKEAAEKGHKIYQEYIKQYE
TSVSEKRSRLETINEQFKVLSEEKLVSERSKTDSENNESEAKRVFEENWK
KLQSENETKLERYKISKAFNELDIDVNERVTFEELQSHHEFDIDQNGDVS
PEEAKEYLSERESVNEIEFTEIVWPNIKQIFKFKERTENDLRHDFEGAVP
DPVDQAPEEEKLPEETPPEEPAKMEYDDETNRLISIANDARNSYNELNEK
FMKLENEKNL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000484secretory system
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR036055LDL_receptor-like_sf
IPR026874Glucosidase_2_bsu
IPR039794Gtb1-like
IPR009011Man6P_isomerase_rcpt-bd_dom_sf
IPR002172LDrepeatLR_classA_rpt
IPR028146PRKCSH_N
IPR011992EF-hand-dom_pair
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
GO:0005509calcium ion binding
Vocabulary: biological process
TermDefinition
GO:0006491N-glycan processing
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 337..361
NoneNo IPR availableCOILSCoilCoilcoord: 146..173
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 296..328
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..17
score: 0.619
IPR036055LDL receptor-like superfamilyGENE3DG3DSA:4.10.400.10coord: 68..108
e-value: 2.8E-5
score: 26.2
IPR028146Glucosidase II beta subunit, N-terminalPFAMPF12999PRKCSH-likecoord: 6..171
e-value: 1.5E-40
score: 138.9
IPR026874Glucosidase 2 subunit betaPANTHERPTHR12630:SF1GLUCOSIDASE 2 SUBUNIT BETAcoord: 4..359
IPR039794Glucosidase II beta subunit-likePANTHERPTHR12630N-LINKED OLIGOSACCHARIDE PROCESSINGcoord: 4..359
IPR002172Low-density lipoprotein (LDL) receptor class A repeatCDDcd00112LDLacoord: 71..106
e-value: 0.00222285
score: 33.3342
IPR011992EF-hand domain pairSUPERFAMILYSSF47473EF-handcoord: 197..263