Neuronal acetylcholine receptor subunit alpha-2

Overview
NameNeuronal acetylcholine receptor subunit alpha-2
Smed IDSMED30019425
Length (bp)216
Neoblast Clusters

Zeng et. al., 2018



 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020



SMED30019425

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pharynxSMED30019425SMESG000015263.1 SMESG000015256.1 dd_Smed_v4_18201_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30019425SMESG000015263.1 SMESG000015256.1 dd_Smed_v4_18201_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30019425SMESG000015263.1 SMESG000015256.1 dd_Smed_v4_18201_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30019425SMESG000015263.1 SMESG000015256.1 dd_Smed_v4_18201_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		whole organism asexual adult colorimetric in situ hybridization evidence
parenchymaSMED30019425SMESG000015263.1 SMESG000015256.1 dd_Smed_v4_18201_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30019425SMESG000015263.1 SMESG000015256.1 dd_Smed_v4_18201_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Neuronal acetylcholine receptor subunit alpha-2 vs. TrEMBL
Match: A0A2H1CG67 (Uncharacterized protein OS=Fasciola hepatica OX=6192 GN=D915_06958 PE=4 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 8.173e-17
Identity = 40/68 (58.82%), Postives = 51/68 (75.00%), Query Frame = 1 
Query:   13 SEVHLESWILQDYDKRSRPVFDNVTTVIRTKDNDVIQQITVEFGLRLIHILDLNEMEQVLTTSVRLLY 216
            SE  L   +L+DYD RSRPV D++T +I+   N  + QI VEFGL L+ ILDL+EM+QVLTTSVR+LY
Sbjct:   93 SEKRLTKNLLKDYDCRSRPVIDHITPLIQVTPNLSVYQINVEFGLELLRILDLDEMDQVLTTSVRMLY 160
[Close]
BLAST of Neuronal acetylcholine receptor subunit alpha-2 vs. TrEMBL
Match: A0A0X3Q4G6 (Neuronal acetylcholine receptor subunit alpha-2 OS=Schistocephalus solidus OX=70667 GN=ACHA2 PE=3 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 1.718e-16
Identity = 39/72 (54.17%), Postives = 53/72 (73.61%), Query Frame = 1 
Query:    1 EGSNSEVHLESWILQDYDKRSRPVFDNVTTVIRTKDNDVIQQITVEFGLRLIHILDLNEMEQVLTTSVRLLY 216
            +GS +E  L   +L DY+K++RPV D V+ V++  +   +QQ+T+EFGL LI ILDLNE EQ+LTTSVR LY
Sbjct:   43 KGSWAEKILLKDLLHDYEKKARPVVDGVSPVVKFSETHNVQQVTIEFGLSLIQILDLNENEQILTTSVRTLY 114
[Close]
BLAST of Neuronal acetylcholine receptor subunit alpha-2 vs. TrEMBL
Match: A0A3P7LL61 (Uncharacterized protein OS=Dibothriocephalus latus OX=60516 GN=DILT_LOCUS7434 PE=4 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 4.725e-16
Identity = 34/60 (56.67%), Postives = 46/60 (76.67%), Query Frame = 1 
Query:   37 ILQDYDKRSRPVFDNVTTVIRTKDNDVIQQITVEFGLRLIHILDLNEMEQVLTTSVRLLY 216
            +L DY+K++RPV D V+ V++  +   +QQ+T+EFGL LI ILDLNE EQ+LTTS R LY
Sbjct:   41 LLHDYEKKARPVVDGVSPVVKFSETHNVQQVTIEFGLSLIQILDLNENEQILTTSFRTLY 100
[Close]
BLAST of Neuronal acetylcholine receptor subunit alpha-2 vs. TrEMBL
Match: A0A3P7CKG5 (Uncharacterized protein OS=Schistocephalus solidus OX=70667 GN=SSLN_LOCUS6753 PE=3 SV=1)

HSP 1 Score: 78.9518 bits (193), Expect = 1.405e-15
Identity = 35/60 (58.33%), Postives = 47/60 (78.33%), Query Frame = 1 
Query:   37 ILQDYDKRSRPVFDNVTTVIRTKDNDVIQQITVEFGLRLIHILDLNEMEQVLTTSVRLLY 216
            +L DY+K++RPV D V+ V++  +   +QQ+T+EFGL LI ILDLNE EQ+LTTSVR LY
Sbjct:    4 LLHDYEKKARPVVDGVSPVVKFSETHNVQQVTIEFGLSLIQILDLNENEQILTTSVRTLY 63
[Close]
BLAST of Neuronal acetylcholine receptor subunit alpha-2 vs. TrEMBL
Match: A0A183SRA5 (Uncharacterized protein OS=Schistocephalus solidus OX=70667 PE=3 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 1.494e-15
Identity = 35/60 (58.33%), Postives = 47/60 (78.33%), Query Frame = 1 
Query:   37 ILQDYDKRSRPVFDNVTTVIRTKDNDVIQQITVEFGLRLIHILDLNEMEQVLTTSVRLLY 216
            +L DY+K++RPV D V+ V++  +   +QQ+T+EFGL LI ILDLNE EQ+LTTSVR LY
Sbjct:    4 LLHDYEKKARPVVDGVSPVVKFSETHNVQQVTIEFGLSLIQILDLNENEQILTTSVRTLY 63
[Close]
BLAST of Neuronal acetylcholine receptor subunit alpha-2 vs. Planmine SMEST
Match: SMESG000015263.1 (SMESG000015263.1)

HSP 1 Score: 138.658 bits (348), Expect = 2.143e-42
Identity = 72/81 (88.89%), Postives = 72/81 (88.89%), Query Frame = 1 
Query:    1 EGSNSEVHLES---------WILQDYDKRSRPVFDNVTTVIRTKDNDVIQQITVEFGLRLIHILDLNEMEQVLTTSVRLLY 216
            EGSNSEVHLES         WILQDYDKRSRPVFDNVTTVIRTKDNDVIQQITVEFGLRLIHILDLNEMEQVLTTSVRLLY
Sbjct:   30 EGSNSEVHLESGWKEKELIQWILQDYDKRSRPVFDNVTTVIRTKDNDVIQQITVEFGLRLIHILDLNEMEQVLTTSVRLLY 110
[Close]
BLAST of Neuronal acetylcholine receptor subunit alpha-2 vs. Planmine SMEST
Match: SMESG000071641.1 (SMESG000071641.1)

HSP 1 Score: 78.5666 bits (192), Expect = 7.412e-19
Identity = 36/60 (60.00%), Postives = 46/60 (76.67%), Query Frame = 1 
Query:   37 ILQDYDKRSRPVFDNVTTVIRTKDNDVIQQITVEFGLRLIHILDLNEMEQVLTTSVRLLY 216
            + ++YDKRSRPV D ++ + R  +N  +QQITVEFG  LI ILDLNEM+QVLTTS R L+
Sbjct:   27 LFENYDKRSRPVVDGISKLSRVSENYTVQQITVEFGFNLIQILDLNEMDQVLTTSTRSLF 86
[Close]
BLAST of Neuronal acetylcholine receptor subunit alpha-2 vs. Planmine SMEST
Match: SMESG000040082.1 (SMESG000040082.1)

HSP 1 Score: 73.559 bits (179), Expect = 4.665e-17
Identity = 41/75 (54.67%), Postives = 53/75 (70.67%), Query Frame = 1 
Query:    1 EGSNSEVHLESWILQDYDKRSRPVFDNVTTVIR---TKDNDVIQQITVEFGLRLIHILDLNEMEQVLTTSVRLLY 216
            E S +E  L + +L  YDKRSRPV DN+T V R    + N  IQQ+TVEFGL LI I+ L+EM+Q+LT++VR LY
Sbjct:   35 EKSWTEKVLINELLNGYDKRSRPVVDNLTPVYRINTAERNQSIQQVTVEFGLSLIQIMKLDEMDQILTSNVRTLY 109
[Close]
BLAST of Neuronal acetylcholine receptor subunit alpha-2 vs. Planmine SMEST
Match: SMESG000071641.1 (SMESG000071641.1)

HSP 1 Score: 66.6254 bits (161), Expect = 1.179e-14
Identity = 30/53 (56.60%), Postives = 39/53 (73.58%), Query Frame = 1 
Query:   37 ILQDYDKRSRPVFDNVTTVIRTKDNDVIQQITVEFGLRLIHILDLNEMEQVLT 195
            + ++YDKRSRPV D ++ + R  +N  +QQITVEFG  LI ILDLNEM+Q  T
Sbjct:   35 LFENYDKRSRPVVDGISKLSRVSENYTVQQITVEFGFNLIQILDLNEMDQRWT 87
The following BLAST results are available for this feature:
BLAST of Neuronal acetylcholine receptor subunit alpha-2 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Neuronal acetylcholine receptor subunit alpha-2 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Neuronal acetylcholine receptor subunit alpha-2 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Neuronal acetylcholine receptor subunit alpha-2 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Neuronal acetylcholine receptor subunit alpha-2 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Neuronal acetylcholine receptor subunit alpha-2 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Neuronal acetylcholine receptor subunit alpha-2 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Neuronal acetylcholine receptor subunit alpha-2 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A2H1CG678.173e-1758.82Uncharacterized protein OS=Fasciola hepatica OX=61... [more]
A0A0X3Q4G61.718e-1654.17Neuronal acetylcholine receptor subunit alpha-2 OS... [more]
A0A3P7LL614.725e-1656.67Uncharacterized protein OS=Dibothriocephalus latus... [more]
A0A3P7CKG51.405e-1558.33Uncharacterized protein OS=Schistocephalus solidus... [more]
A0A183SRA51.494e-1558.33Uncharacterized protein OS=Schistocephalus solidus... [more]
back to top
BLAST of Neuronal acetylcholine receptor subunit alpha-2 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Neuronal acetylcholine receptor subunit alpha-2 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Neuronal acetylcholine receptor subunit alpha-2 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Neuronal acetylcholine receptor subunit alpha-2 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Neuronal acetylcholine receptor subunit alpha-2 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Neuronal acetylcholine receptor subunit alpha-2 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4
Match NameE-valueIdentityDescription
SMESG000015263.12.143e-4288.89SMESG000015263.1[more]
SMESG000071641.17.412e-1960.00SMESG000071641.1[more]
SMESG000040082.14.665e-1754.67SMESG000040082.1[more]
SMESG000071641.11.179e-1456.60SMESG000071641.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30019425 ID=SMED30019425|Name=Neuronal acetylcholine receptor subunit alpha-2|organism=Schmidtea mediterranea sexual|type=transcript|length=216bp
GAAGGTTCTAATTCTGAAGTTCATTTAGAATCGTGGATTTTACAAGACTA
TGATAAACGCTCAAGACCTGTATTTGATAATGTTACAACAGTAATTAGGA
CGAAGGATAATGATGTCATTCAACAAATAACTGTGGAATTCGGACTGAGA
CTCATCCACATTCTCGATCTTAATGAAATGGAACAAGTTCTAACCACTAG
TGTCAGACTGCTTTAT
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0004888transmembrane signaling receptor activity
GO:0005230extracellular ligand-gated ion channel activity
GO:0005216ion channel activity
GO:0022848acetylcholine-gated cation-selective channel activity
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
Vocabulary: INTERPRO
TermDefinition
IPR006202Neur_chan_lig-bd
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0005886plasma membrane
GO:0016021integral component of membrane
GO:0030054cell junction
GO:0045202synapse
GO:0045211postsynaptic membrane
Vocabulary: biological process
TermDefinition
GO:0006810transport
GO:0006811ion transport
GO:0034220ion transmembrane transport
GO:0060078regulation of postsynaptic membrane potential
GO:0060079excitatory postsynaptic potential