SMED30019420

Overview


Name	SMED30019420
Smed ID	SMED30019420
Length (bp)	2374

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of SMED30019420 (SMED30019420) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30019420 (SMED30019420) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30019420 (SMED30019420) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30019420 (SMED30019420) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30019420

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 6

Expressed In	Reference Transcript	Published Transcript	Transcriptome	Publication
tail region	SMED30019420	SmedASXL_072845	SmedAsxl_ww_GCZZ01	PMID:27034770 Currie et al., 2016
pigment cell	SMED30019420	SmedASXL_072845	SmedAsxl_ww_GCZZ01	PMID:29158443 He et al., 2017
epidermis	SMED30019420	dd_Smed_v4_16457_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30019420	dd_Smed_v4_16457_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
tail region	SMED30019420	dd_Smed_v6_16457_0_1	dd_Smed_v6	PMID:28171748 Stückemann et al., 2017
Category 3 cell	SMED30019420	dd_Smed_v4_16457_0_1	dd_Smed_v4	PMID:28292427 Wurtzel et al., 2017

Note: Hover over icons to view figure legend

Homology

[Close]

BLAST of SMED30019420 vs. Ensembl Human
Match: AL136454.1 (novel protein similar to ribosomal protein S27a RPS27A)

HSP 1 Score: 48.521 bits (114), Expect = 6.833e-6
Identity = 30/76 (39.47%), Postives = 43/76 (56.58%), Query Frame = -1

Query: 1466 VQIFIKA-SGETITQDVELQWPMSKIKKIIHQRGEMSYESQKLYRNGKQLNDNKTSSDNNITSGSTIFLLYNQLGG 1690
            +QIF+K   G+TIT +VEL   +  +K  I  +  +  + Q+L   GKQL D +T SD NI   ST+ L+    GG
Sbjct:    1 MQIFVKTLMGKTITLEVELSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76

[Close]

Query: 1466 VQIFIKA-SGETITQDVELQWPMSKIKKIIHQRGEMSYESQKLYRNGKQLNDNKTSSDNNITSGSTIFLLYNQLGG 1690
            +QIF+K   G+TIT +VEL   +  +K  I  +  +  + Q+L   GKQL D +T SD NI   ST+ L+    GG
Sbjct:    1 MQIFVKTLMGKTITLEVELSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76

[Close]

BLAST of SMED30019420 vs. Ensembl Human
Match: UBA52 (ubiquitin A-52 residue ribosomal protein fusion product 1 [Source:HGNC Symbol;Acc:HGNC:12458])

HSP 1 Score: 47.3654 bits (111), Expect = 7.922e-6
Identity = 29/76 (38.16%), Postives = 43/76 (56.58%), Query Frame = -1

Query: 1466 VQIFIKA-SGETITQDVELQWPMSKIKKIIHQRGEMSYESQKLYRNGKQLNDNKTSSDNNITSGSTIFLLYNQLGG 1690
            +QIF+K  +G+TIT +VE    +  +K  I  +  +  + Q+L   GKQL D +T SD NI   ST+ L+    GG
Sbjct:    1 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76

[Close]

Query: 1466 VQIFIKA-SGETITQDVELQWPMSKIKKIIHQRGEMSYESQKLYRNGKQLNDNKTSSDNNITSGSTIFLLYNQLGG 1690
            +QIF+K  +G+TIT +VE    +  +K  I  +  +  + Q+L   GKQL D +T SD NI   ST+ L+    GG
Sbjct:    1 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76

[Close]

Query: 1466 VQIFIKA-SGETITQDVELQWPMSKIKKIIHQRGEMSYESQKLYRNGKQLNDNKTSSDNNITSGSTIFLLYNQLGG 1690
            +QIF+K  +G+TIT +VE    +  +K  I  +  +  + Q+L   GKQL D +T SD NI   ST+ L+    GG
Sbjct:    1 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76

[Close]

BLAST of SMED30019420 vs. Ensembl Celegans
Match: ubq-2 (Ubiquitin-60S ribosomal protein L40 Ubiquitin 60S ribosomal protein L40 [Source:UniProtKB/Swiss-Prot;Acc:P49632])

HSP 1 Score: 47.7506 bits (112), Expect = 2.217e-6
Identity = 29/76 (38.16%), Postives = 43/76 (56.58%), Query Frame = -1

Query: 1466 VQIFIKA-SGETITQDVELQWPMSKIKKIIHQRGEMSYESQKLYRNGKQLNDNKTSSDNNITSGSTIFLLYNQLGG 1690
            +QIF+K  +G+TIT +VE    +  +K  I  +  +  + Q+L   GKQL D +T SD NI   ST+ L+    GG
Sbjct:    1 MQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76

[Close]

BLAST of SMED30019420 vs. Ensembl Fly
Match: RpL40 (gene:FBgn0003941 transcript:FBtr0334787)

HSP 1 Score: 47.3654 bits (111), Expect = 3.869e-6
Identity = 29/76 (38.16%), Postives = 43/76 (56.58%), Query Frame = -1

Query: 1466 VQIFIKA-SGETITQDVELQWPMSKIKKIIHQRGEMSYESQKLYRNGKQLNDNKTSSDNNITSGSTIFLLYNQLGG 1690
            +QIF+K  +G+TIT +VE    +  +K  I  +  +  + Q+L   GKQL D +T SD NI   ST+ L+    GG
Sbjct:    1 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76

[Close]

BLAST of SMED30019420 vs. Ensembl Fly
Match: RpL40 (gene:FBgn0003941 transcript:FBtr0077470)

HSP 1 Score: 47.3654 bits (111), Expect = 3.869e-6
Identity = 29/76 (38.16%), Postives = 43/76 (56.58%), Query Frame = -1

Query: 1466 VQIFIKA-SGETITQDVELQWPMSKIKKIIHQRGEMSYESQKLYRNGKQLNDNKTSSDNNITSGSTIFLLYNQLGG 1690
            +QIF+K  +G+TIT +VE    +  +K  I  +  +  + Q+L   GKQL D +T SD NI   ST+ L+    GG
Sbjct:    1 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76

[Close]

BLAST of SMED30019420 vs. Ensembl Fly
Match: CG11700 (gene:FBgn0029856 transcript:FBtr0333520)

HSP 1 Score: 49.6766 bits (117), Expect = 5.294e-6
Identity = 30/78 (38.46%), Postives = 45/78 (57.69%), Query Frame = -1

Query: 1460 VQIFIKA-SGETITQDVELQWPMSKIKKIIHQRGEMSYESQKLYRNGKQLNDNKTSSDNNITSGSTIFLLYNQLGGTE 1690
            +QIF+K  +G+TIT +VE    +  +K  IH +  +  + Q+L   GKQL D +T SD NI   ST+ L+    GG +
Sbjct:  153 MQIFVKTLTGKTITLEVEPSDSIENVKARIHDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQ 230

[Close]

BLAST of SMED30019420 vs. Ensembl Fly
Match: RpS27A (gene:FBgn0003942 transcript:FBtr0080016)

HSP 1 Score: 46.9802 bits (110), Expect = 9.889e-6
Identity = 29/77 (37.66%), Postives = 43/77 (55.84%), Query Frame = -1

Query: 1463 VQIFIKA-SGETITQDVELQWPMSKIKKIIHQRGEMSYESQKLYRNGKQLNDNKTSSDNNITSGSTIFLLYNQLGGT 1690
            +QIF+K  +G+TIT +VE    +  +K  I  +  +  + Q+L   GKQL D +T SD NI   ST+ L+    GG 
Sbjct:    1 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGA 77

[Close]

BLAST of SMED30019420 vs. Ensembl Zebrafish
Match: ubb (ubiquitin B [Source:ZFIN;Acc:ZDB-GENE-050411-10])

HSP 1 Score: 46.595 bits (109), Expect = 3.867e-6
Identity = 29/78 (37.18%), Postives = 44/78 (56.41%), Query Frame = -1

Query: 1460 VQIFIKA-SGETITQDVELQWPMSKIKKIIHQRGEMSYESQKLYRNGKQLNDNKTSSDNNITSGSTIFLLYNQLGGTE 1690
            +QIF+K  +G+TIT +VE    +  +K  I  +  +  + Q+L   GKQL D +T SD NI   ST+ L+    GG +
Sbjct:    1 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGAQ 78

[Close]

BLAST of SMED30019420 vs. Ensembl Zebrafish
Match: uba52 (ubiquitin A-52 residue ribosomal protein fusion product 1 [Source:ZFIN;Acc:ZDB-GENE-051023-7])

HSP 1 Score: 47.3654 bits (111), Expect = 4.991e-6
Identity = 29/76 (38.16%), Postives = 43/76 (56.58%), Query Frame = -1

Query: 1466 VQIFIKA-SGETITQDVELQWPMSKIKKIIHQRGEMSYESQKLYRNGKQLNDNKTSSDNNITSGSTIFLLYNQLGG 1690
            +QIF+K  +G+TIT +VE    +  +K  I  +  +  + Q+L   GKQL D +T SD NI   ST+ L+    GG
Sbjct:    1 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76

[Close]

BLAST of SMED30019420 vs. Ensembl Xenopus
Match: uba52 (ubiquitin A-52 residue ribosomal protein fusion product 1 [Source:NCBI gene;Acc:448705])

HSP 1 Score: 47.3654 bits (111), Expect = 7.181e-6
Identity = 29/76 (38.16%), Postives = 43/76 (56.58%), Query Frame = -1

Query: 1466 VQIFIKA-SGETITQDVELQWPMSKIKKIIHQRGEMSYESQKLYRNGKQLNDNKTSSDNNITSGSTIFLLYNQLGG 1690
            +QIF+K  +G+TIT +VE    +  +K  I  +  +  + Q+L   GKQL D +T SD NI   ST+ L+    GG
Sbjct:    1 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76

[Close]

BLAST of SMED30019420 vs. Ensembl Mouse
Match: Gm11808 (predicted gene 11808 [Source:MGI Symbol;Acc:MGI:3649356])

HSP 1 Score: 47.3654 bits (111), Expect = 5.439e-6
Identity = 29/76 (38.16%), Postives = 43/76 (56.58%), Query Frame = -1

Query: 1466 VQIFIKA-SGETITQDVELQWPMSKIKKIIHQRGEMSYESQKLYRNGKQLNDNKTSSDNNITSGSTIFLLYNQLGG 1690
            +QIF+K  +G+TIT +VE    +  +K  I  +  +  + Q+L   GKQL D +T SD NI   ST+ L+    GG
Sbjct:    1 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76

[Close]

BLAST of SMED30019420 vs. Ensembl Mouse
Match: Uba52 (ubiquitin A-52 residue ribosomal protein fusion product 1 [Source:MGI Symbol;Acc:MGI:98887])

HSP 1 Score: 47.3654 bits (111), Expect = 5.439e-6
Identity = 29/76 (38.16%), Postives = 43/76 (56.58%), Query Frame = -1

Query: 1466 VQIFIKA-SGETITQDVELQWPMSKIKKIIHQRGEMSYESQKLYRNGKQLNDNKTSSDNNITSGSTIFLLYNQLGG 1690
            +QIF+K  +G+TIT +VE    +  +K  I  +  +  + Q+L   GKQL D +T SD NI   ST+ L+    GG
Sbjct:    1 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76

[Close]

Query: 1466 VQIFIKA-SGETITQDVELQWPMSKIKKIIHQRGEMSYESQKLYRNGKQLNDNKTSSDNNITSGSTIFLLYNQLGG 1690
            +QIF+K  +G+TIT +VE    +  +K  I  +  +  + Q+L   GKQL D +T SD NI   ST+ L+    GG
Sbjct:    1 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76

[Close]

Query: 1466 VQIFIKA-SGETITQDVELQWPMSKIKKIIHQRGEMSYESQKLYRNGKQLNDNKTSSDNNITSGSTIFLLYNQLGG 1690
            +QIF+K  +G+TIT +VE    +  +K  I  +  +  + Q+L   GKQL D +T SD NI   ST+ L+    GG
Sbjct:    1 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76

[Close]

Query: 1466 VQIFIKA-SGETITQDVELQWPMSKIKKIIHQRGEMSYESQKLYRNGKQLNDNKTSSDNNITSGSTIFLLYNQLGG 1690
            +QIF+K  +G+TIT +VE    +  +K  I  +  +  + Q+L   GKQL D +T SD NI   ST+ L+    GG
Sbjct:    1 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76

[Close]

BLAST of SMED30019420 vs. UniProt/SwissProt
Match: sp|P0DJ25|RL40_TETTS (Ubiquitin-60S ribosomal protein L40 OS=Tetrahymena thermophila (strain SB210) OX=312017 GN=RPL40 PE=1 SV=1)

HSP 1 Score: 52.7582 bits (125), Expect = 5.288e-7
Identity = 36/109 (33.03%), Postives = 56/109 (51.38%), Query Frame = -1

Query: 1367 VQIFIKA-SGETITQDVELQWPMSKIKKIIHQRGEMSYESQKLYRNGKQLNDNKTSSDNNITSGSTIFLLYNQLGGTETEEISGRKKRQSQKWRLTRKRNILLAAISTN 1690
            +QIF+K  +G+TIT DVE    +  +K  I  +  +  + Q+L   GKQL D +T SD NI   ST+ L+    GG     I+   K+ + + ++ R     L   +TN
Sbjct:    1 MQIFVKTLTGKTITLDVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGGMEPTIAALAKKYNCEKKVCRDCYARLPPKATN 109

[Close]

BLAST of SMED30019420 vs. UniProt/SwissProt
Match: sp|P33190|RL40_TETPY (Ubiquitin-60S ribosomal protein L40 OS=Tetrahymena pyriformis OX=5908 GN=TUF11 PE=3 SV=2)

HSP 1 Score: 52.7582 bits (125), Expect = 5.288e-7
Identity = 36/109 (33.03%), Postives = 56/109 (51.38%), Query Frame = -1

Query: 1367 VQIFIKA-SGETITQDVELQWPMSKIKKIIHQRGEMSYESQKLYRNGKQLNDNKTSSDNNITSGSTIFLLYNQLGGTETEEISGRKKRQSQKWRLTRKRNILLAAISTN 1690
            +QIF+K  +G+TIT DVE    +  +K  I  +  +  + Q+L   GKQL D +T SD NI   ST+ L+    GG     I+   K+ + + ++ R     L   +TN
Sbjct:    1 MQIFVKTLTGKTITLDVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGGMEPTIAALAKKYNCEKKVCRDCYARLPPKATN 109

[Close]

BLAST of SMED30019420 vs. UniProt/SwissProt
Match: sp|P46575|RL40_EIMBO (Ubiquitin-60S ribosomal protein L40 OS=Eimeria bovis OX=5803 PE=2 SV=2)

HSP 1 Score: 50.0618 bits (118), Expect = 4.386e-6
Identity = 35/109 (32.11%), Postives = 56/109 (51.38%), Query Frame = -1

Query: 1367 VQIFIKA-SGETITQDVELQWPMSKIKKIIHQRGEMSYESQKLYRNGKQLNDNKTSSDNNITSGSTIFLLYNQLGGTETEEISGRKKRQSQKWRLTRKRNILLAAISTN 1690
            +QIF+K  +G+TIT DVE    +  +K  I  +  +  + Q+L   GKQL D +T SD NI   ST+ L+    GG     ++    + + + ++ RK    L   +TN
Sbjct:    1 MQIFVKTLTGKTITLDVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGIIEPSLALLASKYNCEKKICRKCYARLPPRATN 109

[Close]

BLAST of SMED30019420 vs. Ensembl Cavefish
Match: uba52 (ubiquitin A-52 residue ribosomal protein fusion product 1 [Source:NCBI gene;Acc:103027541])

HSP 1 Score: 47.3654 bits (111), Expect = 4.230e-6
Identity = 29/76 (38.16%), Postives = 43/76 (56.58%), Query Frame = -1

Query: 1466 VQIFIKA-SGETITQDVELQWPMSKIKKIIHQRGEMSYESQKLYRNGKQLNDNKTSSDNNITSGSTIFLLYNQLGG 1690
            +QIF+K  +G+TIT +VE    +  +K  I  +  +  + Q+L   GKQL D +T SD NI   ST+ L+    GG
Sbjct:    1 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76

[Close]

Query: 1466 VQIFIKA-SGETITQDVELQWPMSKIKKIIHQRGEMSYESQKLYRNGKQLNDNKTSSDNNITSGSTIFLLYNQLGG 1690
            +QIF+K  +G+TIT +VE    +  +K  I  +  +  + Q+L   GKQL D +T SD NI   ST+ L+    GG
Sbjct:    1 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76

[Close]

BLAST of SMED30019420 vs. Ensembl Yeast
Match: RPL40B (Ubiquitin-ribosomal 60S subunit protein L40B fusion protein; cleaved to yield ubiquitin and ribosomal protein L40B; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40B has a paralog, RPL40A, that arose from the whole genome duplication [Source:SGD;Acc:S000001802])

HSP 1 Score: 47.3654 bits (111), Expect = 6.092e-7
Identity = 29/76 (38.16%), Postives = 43/76 (56.58%), Query Frame = -1

Query: 1466 VQIFIKA-SGETITQDVELQWPMSKIKKIIHQRGEMSYESQKLYRNGKQLNDNKTSSDNNITSGSTIFLLYNQLGG 1690
            +QIF+K  +G+TIT +VE    +  +K  I  +  +  + Q+L   GKQL D +T SD NI   ST+ L+    GG
Sbjct:    1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76

[Close]

BLAST of SMED30019420 vs. Ensembl Yeast
Match: RPL40A (Ubiquitin-ribosomal 60S subunit protein L40A fusion protein; cleaved to yield ubiquitin and ribosomal protein L40A; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40A has a paralog, RPL40B, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress [Source:SGD;Acc:S000001410])

HSP 1 Score: 47.3654 bits (111), Expect = 6.092e-7
Identity = 29/76 (38.16%), Postives = 43/76 (56.58%), Query Frame = -1

Query: 1466 VQIFIKA-SGETITQDVELQWPMSKIKKIIHQRGEMSYESQKLYRNGKQLNDNKTSSDNNITSGSTIFLLYNQLGG 1690
            +QIF+K  +G+TIT +VE    +  +K  I  +  +  + Q+L   GKQL D +T SD NI   ST+ L+    GG
Sbjct:    1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76

[Close]

BLAST of SMED30019420 vs. Ensembl Nematostella
Match: EDO45561 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RSG9])

HSP 1 Score: 46.2098 bits (108), Expect = 5.745e-6
Identity = 29/78 (37.18%), Postives = 44/78 (56.41%), Query Frame = -1

Query: 1460 VQIFIKA-SGETITQDVELQWPMSKIKKIIHQRGEMSYESQKLYRNGKQLNDNKTSSDNNITSGSTIFLLYNQLGGTE 1690
            +QIF+K  +G+TIT +VE    +  +K  I  +  +  + Q+L   GKQL D +T SD NI   ST+ L+    GG +
Sbjct:    1 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQ 78

[Close]

BLAST of SMED30019420 vs. Ensembl Nematostella
Match: EDO48503 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RJ25])

HSP 1 Score: 46.595 bits (109), Expect = 5.879e-6
Identity = 29/77 (37.66%), Postives = 43/77 (55.84%), Query Frame = -1

Query: 1463 VQIFIKA-SGETITQDVELQWPMSKIKKIIHQRGEMSYESQKLYRNGKQLNDNKTSSDNNITSGSTIFLLYNQLGGT 1690
            +QIF+K  +G+TIT +VE    +  +K  I  +  +  + Q+L   GKQL D +T SD NI   ST+ L+    GG 
Sbjct:    1 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGA 77

[Close]

BLAST of SMED30019420 vs. Ensembl Medaka
Match: ENSORLT00000015052.2 (pep primary_assembly:ASM223467v1:12:2267197:2268484:1 gene:ENSORLG00000012019.2 transcript:ENSORLT00000015052.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 47.7506 bits (112), Expect = 3.276e-6
Identity = 32/91 (35.16%), Postives = 49/91 (53.85%), Query Frame = -1

Query: 1421 VQIFIKA-SGETITQDVELQWPMSKIKKIIHQRGEMSYESQKLYRNGKQLNDNKTSSDNNITSGSTIFLLYNQLGGTETEEISGRKKRQSQ 1690
            +QIF+K  +G+TIT +VE    +  +K  I  +  +  + Q+L   GKQL D +T SD NI   ST+ L+    G T+    +GR   + Q
Sbjct:    1 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLQTPGSTDA---NGRSPAEKQ 88

[Close]

BLAST of SMED30019420 vs. Ensembl Medaka
Match: uba52 (ubiquitin A-52 residue ribosomal protein fusion product 1 [Source:NCBI gene;Acc:101169681])

HSP 1 Score: 47.7506 bits (112), Expect = 3.568e-6
Identity = 29/76 (38.16%), Postives = 43/76 (56.58%), Query Frame = -1

Query: 1466 VQIFIKA-SGETITQDVELQWPMSKIKKIIHQRGEMSYESQKLYRNGKQLNDNKTSSDNNITSGSTIFLLYNQLGG 1690
            +QIF+K  +G+TIT +VE    +  +K  I  +  +  + Q+L   GKQL D +T SD NI   ST+ L+    GG
Sbjct:    1 MQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76

[Close]

BLAST of SMED30019420 vs. Planmine SMEST
Match: SMESG000010152.1 (SMESG000010152.1)

HSP 1 Score: 317.005 bits (811), Expect = 2.718e-102
Identity = 193/266 (72.56%), Postives = 212/266 (79.70%), Query Frame = -1

Query: 1145 RLGSNETESPVGNSLIKPDXXXXXXXKPFSMKPRIEVSKVNRFTCKIKRNEVTTGESVKDNEVENANNENLSGEEICIGAKQQNVQIFIKASGETITQDVELQWPMSKIKKIIHQRGEMSYESQKLYRNGKQLNDNKTSSDNNITSGSTIFLLYNQLGGTETEEISGRKKRQSQKWRLTRKRNILLAAISTNDCLKLAEESEGKFYKMNESFIEFLDRNKKVGKKEVKEILEKDGSEIYQKKEKVD*ILENNAERVFSNKDYKSRK 1942
            RLGSNETESPVGNSLIKPDNK NKKNK FS+K RIEVSKVNRFT KIKRNEVTTGE VK NEVENANNENLSGEE C+G  QQNVQIFIKAS ETIT DVELQWP+SKIKKIIHQRG +SY SQKLYRNG+QLNDNKT  DN IT G+                I   K+ QSQKWRLTRKRN+LLAAIST+DC KL EE E +FYK+ ES+ + LD ++K  KKEVKEILEKD SEI QK EKV  +LENNAER  S+KD+KS+K
Sbjct:  170 RLGSNETESPVGNSLIKPDNKSNKKNKTFSVKKRIEVSKVNRFTFKIKRNEVTTGECVKVNEVENANNENLSGEENCMGVNQQNVQIFIKASEETITLDVELQWPISKIKKIIHQRGGVSYGSQKLYRNGEQLNDNKTLLDNFITKGT----------------IRRIKRCQSQKWRLTRKRNLLLAAISTDDCPKLVEEYEEEFYKVIESWTKLLDFSEKAVKKEVKEILEKDISEIDQKNEKVHQLLENNAERACSSKDFKSQK 419

HSP 2 Score: 62.003 bits (149), Expect = 2.718e-102
Identity = 29/44 (65.91%), Postives = 35/44 (79.55%), Query Frame = -2

Query:  931 MKDNEQWKNGFNPFVDSTSANGGEKLMHLRQYMVGESLRIIEYL 1062
            MKD E+ +N F+ +VDS SAN GE LM LRQY+VGE LRIIE+L
Sbjct:  448 MKDYERRRNEFDAYVDSGSANDGENLMRLRQYLVGEPLRIIEHL 491

HSP 3 Score: 36.1946 bits (82), Expect = 2.718e-102
Identity = 20/29 (68.97%), Postives = 20/29 (68.97%), Query Frame = -3

Query: 1062 KSLKENHSMESIKGLDKLNKIVIKPFTCQ 1148
            K LKEN SMES K L KL KIVIK F  Q
Sbjct:  419 KRLKENKSMESFKCLGKLKKIVIKQFAGQ 447

[Close]

BLAST of SMED30019420 vs. Planmine SMEST
Match: SMESG000071974.1 (SMESG000071974.1)

HSP 1 Score: 207.223 bits (526), Expect = 2.695e-63
Identity = 97/98 (98.98%), Postives = 97/98 (98.98%), Query Frame = -2

Query:  769 MKDNEQWKNGFNPFVDSTSANGGEKLMHLRQYMVGESLRIIEYLGNHTPVHNMAQETLETRYRGDDMRYCGILAKIKEFYPVSQKQNYFTVSSFGFSR 1062
            MKDNEQWKNGFN FVDSTSANGGEKLMHLRQYMVGESLRIIEYLGNHTPVHNMAQETLETRYRGDDMRYCGILAKIKEFYPVSQKQNYFTVSSFGFSR
Sbjct:    1 MKDNEQWKNGFNLFVDSTSANGGEKLMHLRQYMVGESLRIIEYLGNHTPVHNMAQETLETRYRGDDMRYCGILAKIKEFYPVSQKQNYFTVSSFGFSR 98

[Close]

BLAST of SMED30019420 vs. Planmine SMEST
Match: SMESG000046963.1 (SMESG000046963.1)

HSP 1 Score: 206.838 bits (525), Expect = 2.056e-60
Identity = 111/131 (84.73%), Postives = 115/131 (87.79%), Query Frame = -1

Query: 1550 RLGSNETESPVGNSLIKPDXXXXXXXKPFSMKPRIEVSKVNRFTCKIKRNEVTTGESVKDNEVENANNENLSGEEICIGAKQQNVQIFIKASGETITQDVELQWPMSKIKKIIHQRGEMSYESQKLYRNGK 1942
            RLGSNETESPVGNSLIKPD K NKKNK FSM  RIEVSKVN FT KIKRNEVTTGE VKDNEVENANN+NLSG+E CI AKQQNVQIFI ASG TIT DVELQWP+SKIKKIIHQRG MSY SQKLY NG+
Sbjct:   42 RLGSNETESPVGNSLIKPDKKSNKKNKTFSMTTRIEVSKVNIFTFKIKRNEVTTGECVKDNEVENANNKNLSGKENCISAKQQNVQIFISASGGTITLDVELQWPISKIKKIIHQRGGMSYGSQKLYHNGQ 172

HSP 2 Score: 105.145 bits (261), Expect = 1.940e-24
Identity = 51/82 (62.20%), Postives = 61/82 (74.39%), Query Frame = -2

Query:  817 MKDNEQWKNGFNPFVDSTSANGGEKLMHLRQYMVGESLRIIEYLGNHTPVHNMAQETLETRYRGDDMRYCGILAKIKEFYPV 1062
            +K+ E+W+N F+ +VDS SAN GEKLMHLRQY+VGE LRIIE LG    V+  A E LE RY GDD R  G+LA+IKEFY V
Sbjct:  173 LKNYERWRNEFDAYVDSASANDGEKLMHLRQYLVGEPLRIIENLGYEAAVYKTALERLEMRYGGDDRRCHGMLAEIKEFYIV 254

[Close]

BLAST of SMED30019420 vs. Planmine SMEST
Match: SMESG000047744.1 (SMESG000047744.1)

HSP 1 Score: 93.9745 bits (232), Expect = 2.892e-28
Identity = 55/87 (63.22%), Postives = 63/87 (72.41%), Query Frame = -2

Query: 1984 DVANGSSFQGITAYGHESYRISLKIDVKIKCLIKVAVGRWGGRILKLYGFKSSVNGTYNLPAVSDNISESENITIQIQKQNKEVKRY 2244
            D +  SSF  +    HE+Y ISLK DVKIKC+IKVAV R     LKL  FKSS NGTY L AV +NISE+ENITIQ QKQNKE+KR+
Sbjct:  151 DSSKRSSFHHV----HENYGISLKTDVKIKCVIKVAVERGE---LKLSEFKSSANGTYYLSAVYENISENENITIQEQKQNKELKRH 230

HSP 2 Score: 51.9878 bits (123), Expect = 2.892e-28
Identity = 32/39 (82.05%), Postives = 34/39 (87.18%), Query Frame = -1

Query: 1826 RLGSNETESPVGNSLIKPDXXXXXXXKPFSMKPRIEVSK 1942
            R GSNET+S +GNSLIKPDNK NKKNK FSMK RIEVSK
Sbjct:  261 RFGSNETDSRLGNSLIKPDNKSNKKNKTFSMKTRIEVSK 299

HSP 3 Score: 61.6178 bits (148), Expect = 6.607e-10
Identity = 28/36 (77.78%), Postives = 33/36 (91.67%), Query Frame = -1

Query: 2246 ECTKVTSDGISEMAKSFSKVKFMNCN*VVQLITENV 2353
            EC KVTSDGISEMAKS+SKV+FMNCN V++LIT+N 
Sbjct:   15 ECAKVTSDGISEMAKSWSKVRFMNCNYVIELITKNA 50

[Close]

BLAST of SMED30019420 vs. Planmine SMEST
Match: SMESG000042730.1 (SMESG000042730.1)

HSP 1 Score: 101.679 bits (252), Expect = 4.957e-25
Identity = 50/88 (56.82%), Postives = 62/88 (70.45%), Query Frame = -2

Query:  817 KTIYMSMKDNEQWKNGFNPFVDSTSANGGEKLMHLRQYMVGESLRIIEYLGNHTPVHNMAQETLETRYRGDDMRYCGILAKIKEFYPV 1080
            K+    +KD + W+N F+  VDS S N GEKLMHLRQY+VG+ L+IIE+LG    V+N A E LE  Y GDD RY  +LA+IKEFYPV
Sbjct:   16 KSFEGQLKDYKLWRNEFDACVDSASVNDGEKLMHLRQYLVGDPLQIIEHLGYDAAVYNTALERLEMMYGGDDRRYRRMLAEIKEFYPV 103

The following BLAST results are available for this feature:

BLAST of SMED30019420 vs. Ensembl Human
BLAST of SMED30019420 vs. Ensembl Celegans
BLAST of SMED30019420 vs. Ensembl Fly
BLAST of SMED30019420 vs. Ensembl Zebrafish
BLAST of SMED30019420 vs. Ensembl Xenopus
BLAST of SMED30019420 vs. Ensembl Mouse
BLAST of SMED30019420 vs. UniProt/SwissProt
BLAST of SMED30019420 vs. TrEMBL
BLAST of SMED30019420 vs. Ensembl Cavefish
BLAST of SMED30019420 vs. Ensembl Sea Lamprey
BLAST of SMED30019420 vs. Ensembl Yeast
BLAST of SMED30019420 vs. Ensembl Nematostella
BLAST of SMED30019420 vs. Ensembl Medaka
BLAST of SMED30019420 vs. Planmine SMEST

BLAST of SMED30019420 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
AL136454.1	6.833e-6	39.47	novel protein similar to ribosomal protein S27a RP...	[more]
AL136454.1	6.833e-6	39.47	novel protein similar to ribosomal protein S27a RP...	[more]
UBA52	7.922e-6	38.16	ubiquitin A-52 residue ribosomal protein fusion pr...	[more]
UBA52	7.922e-6	38.16	ubiquitin A-52 residue ribosomal protein fusion pr...	[more]
UBA52	7.922e-6	38.16	ubiquitin A-52 residue ribosomal protein fusion pr...	[more]

BLAST of SMED30019420 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1


Match Name	E-value	Identity	Description
ubq-2	2.217e-6	38.16	Ubiquitin-60S ribosomal protein L40 Ubiquitin 60S ...	[more]

BLAST of SMED30019420 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 4


Match Name	E-value	Identity	Description
RpL40	3.869e-6	38.16	gene:FBgn0003941 transcript:FBtr0334787	[more]
RpL40	3.869e-6	38.16	gene:FBgn0003941 transcript:FBtr0077470	[more]
CG11700	5.294e-6	38.46	gene:FBgn0029856 transcript:FBtr0333520	[more]
RpS27A	9.889e-6	37.66	gene:FBgn0003942 transcript:FBtr0080016	[more]

BLAST of SMED30019420 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2


Match Name	E-value	Identity	Description
ubb	3.867e-6	37.18	ubiquitin B [Source:ZFIN;Acc:ZDB-GENE-050411-10]	[more]
uba52	4.991e-6	38.16	ubiquitin A-52 residue ribosomal protein fusion pr...	[more]

BLAST of SMED30019420 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1


Match Name	E-value	Identity	Description
uba52	7.181e-6	38.16	ubiquitin A-52 residue ribosomal protein fusion pr...	[more]

BLAST of SMED30019420 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Gm11808	5.439e-6	38.16	predicted gene 11808 [Source:MGI Symbol;Acc:MGI:36...	[more]
Uba52	5.439e-6	38.16	ubiquitin A-52 residue ribosomal protein fusion pr...	[more]
Uba52	5.439e-6	38.16	ubiquitin A-52 residue ribosomal protein fusion pr...	[more]
Uba52	5.439e-6	38.16	ubiquitin A-52 residue ribosomal protein fusion pr...	[more]
Uba52	5.439e-6	38.16	ubiquitin A-52 residue ribosomal protein fusion pr...	[more]

BLAST of SMED30019420 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 3


Match Name	E-value	Identity	Description
sp\|P0DJ25\|RL40_TETTS	5.288e-7	33.03	Ubiquitin-60S ribosomal protein L40 OS=Tetrahymena...	[more]
sp\|P33190\|RL40_TETPY	5.288e-7	33.03	Ubiquitin-60S ribosomal protein L40 OS=Tetrahymena...	[more]
sp\|P46575\|RL40_EIMBO	4.386e-6	32.11	Ubiquitin-60S ribosomal protein L40 OS=Eimeria bov...	[more]

BLAST of SMED30019420 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30019420 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2


Match Name	E-value	Identity	Description
uba52	4.230e-6	38.16	ubiquitin A-52 residue ribosomal protein fusion pr...	[more]
uba52	4.230e-6	38.16	ubiquitin A-52 residue ribosomal protein fusion pr...	[more]

BLAST of SMED30019420 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30019420 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 2


Match Name	E-value	Identity	Description
RPL40B	6.092e-7	38.16	Ubiquitin-ribosomal 60S subunit protein L40B fusio...	[more]
RPL40A	6.092e-7	38.16	Ubiquitin-ribosomal 60S subunit protein L40A fusio...	[more]

BLAST of SMED30019420 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2


Match Name	E-value	Identity	Description
EDO45561	5.745e-6	37.18	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO48503	5.879e-6	37.66	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of SMED30019420 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2


Match Name	E-value	Identity	Description
ENSORLT00000015052.2	3.276e-6	35.16	pep primary_assembly:ASM223467v1:12:2267197:226848...	[more]
uba52	3.568e-6	38.16	ubiquitin A-52 residue ribosomal protein fusion pr...	[more]

BLAST of SMED30019420 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000010152.1	2.718e-102	72.56	SMESG000010152.1	[more]
SMESG000071974.1	2.695e-63	98.98	SMESG000071974.1	[more]
SMESG000046963.1	2.056e-60	84.73	SMESG000046963.1	[more]
SMESG000047744.1	2.892e-28	63.22	SMESG000047744.1	[more]
SMESG000042730.1	4.957e-25	56.82	SMESG000042730.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence
protein sequence
protein sequence

transcript sequence

>SMED30019420 ID=SMED30019420|Name=SMED30019420|organism=Schmidtea mediterranea sexual|type=transcript|length=2374bp
CAGCCAAATCATATTATATTTCAAAAACAATAAAGGGTGACATTTTTTGG
GTATCAATCTTGGGGTTTTTGACGAATTTATTGAATCAAGTGTATTTATT
GTGCTCTTTCAATAAAATTATATTAAGTTACTATTCGCCTGAACCCGAGG
ACCTAGATAGGAATATAATTCCAGCCGATTTTATATTAATATCCAAAGTT
GGAATATATTCTTAAAAATTAAATTCAAAAGTATTTCTACAATTCGTAGC
AAGACTTGAATTTCATTTTGAATGATATTTGAAAAGTACACTACACGATT
TGTTCAACAAAACCATTCAACTTTCAAATTTAAGATACAGACGAAAAAAC
TGCTTTTGACGAAAGTTTACAGTGACACCTTTGTAAGAACTGAGTCAATC
TTTTGTGACTAAATTTCCAAATATTATTAACGCCATTTTGAAATTTCAAT
TACGTTCTTTACTAAAAATAAAACCTAACCTTGATATTAAATGACGCTTT
TCGTTACACAAAACCATATAATGTGACGAATAGTGTCCTACAGTCTACAG
TTGAAGATTTAAGTCGTAATATTCTGTGTAATCGTAAACAACAAAATAAT
TGTTTCCATTTCCATTGAGTATCAACATCCATTTTATCAACAATTAGTTT
GTACTGAGTGGAAAAGTCTATTGAATTAATCCTGCCCATTGCAACTAGAC
ATGCAAATATTGTTCTATAATTTATTAGCAATTAATGCTCATTTTTGGAT
TGCCTATAGCAATTTTTGACGACTAAATCCGAAGGAGGAAACAGTAAAAT
AATTTTGTTTTTGTGATACAGGATAGAATTCTTTTATTTTTGCTAGTATT
CCGCAGTATCTCATGTCATCTCCTCGGTACCTCGTCTCTAGTGTTTCTTG
AGCCATGTTGTGAACTGGAGTATGATTTCCTAAATACTCTATTATCCGCA
GAGATTCTCCCACCATGTATTGTCTTAAATGCATCAGCTTTTCTCCACCA
TTGGCACTTGTTGAGTCAACAAAAGGGTTAAATCCATTTTTCCACTGTTC
ATTATCCTTCATTGACATGTAAATGGTTTTATTACAATTTTATTTAGTTT
GTCCAGACCTTTAATGGATTCCATGCTATGATTTTCCTTTAAACTTTTCG
CGATTTATAATCTTTGTTACTAAATACTCTTTCCGCATTATTCTCCAGTA
TTCAATCGACCTTTTCCTTTTTCTGATAAATTTCCGACCCATCCTTTTCT
AAAATCTCTTTAACTTCCTTTTTCCCTACCTTTTTATTACGATCTAAAAA
CTCAATGAAACTTTCGTTCATTTTATAAAACTTCCCTTCAGATTCTTCCG
CTAACTTAAGGCAATCATTCGTTGAAATTGCTGCTAATAATATATTTCGC
TTTCTTGTTAACCGCCATTTCTGTGATTGGCGCTTCTTTCGTCCTGATAT
CTCCTCAGTTTCCGTTCCCCCCAACTGGTTGTAGAGCAGAAAGATTGTTG
ATCCACTTGTAATATTATTATCTGATGACGTCTTGTTATCATTTAATTGT
TTACCATTACGATATAGTTTTTGGCTTTCATACGACATCTCTCCTCTTTG
GTGAATTATTTTCTTGATTTTTGACATCGGCCATTGTAACTCAACATCTT
GTGTGATGGTTTCTCCTGATGCTTTAATAAATATTTGCACATTTTGTTGT
TTGGCACCTATGCAAATTTCTTCACCTGACAAATTTTCATTGTTAGCATT
CTCTACTTCATTGTCTTTTACACTTTCACCAGTTGTCACTTCGTTACGTT
TGATCTTACAAGTAAATCTATTTACTTTTGAAACCTCGATTCTCGGTTTC
ATAGAGAATGGTTTATTTTTCTTATTTTATTTGTTGTCGGGCTTTATCAA
TGAATTCCCAACTGGAGATTCAGTTTCATTGCTTCCCAACCTTTATTGTT
TTTAATTTCAATTTTCAGTTTTTTGATAGTTTTATATCTTTTCACTTCTT
TATTCTGTTTTTGTATTTGAATAGTAATATTTTCGCTCTCGCTAATATTA
TCAGACACAGCAGGTAAATTATAAGTGCCGTTAACACTAGATTTGAATCC
ATAAAGCTTTAATATTCTCCCCCCCCACCTTCCAACAGCTACTTTAATTA
AACATTTAATTTTGACATCAATTTTAAGAGATATTCTATAACTCTCATGA
CCATACGCTGTTATTCCTTGAAATGATGACCCATTTGCCACATCTGACAT
TTTCTGTGATTAATTGTACGACTTAATTACAATTCATAAATTTTACTTTA
GAGAAAGACTTAGCCATCTCTGAAATCCCATCACTTGTTACTTTTGTACA
TTCATCAGAAATATCACAGAACGA

protein sequence of SMED30019420-orf-1

>SMED30019420-orf-1 ID=SMED30019420-orf-1|Name=SMED30019420-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=105bp
MSMKDNEQWKNGFNPFVDSTSANGGEKLMHLRQYMVGESLRIIEYLGNHT
PVHNMAQETLETRYRGDDMRYCGILAKIKEFYPVSQKQNYFTVSSFGFSR
QKLL*

protein sequence of SMED30019420-orf-2

>SMED30019420-orf-2 ID=SMED30019420-orf-2|Name=SMED30019420-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=118bp
MSDVANGSSFQGITAYGHESYRISLKIDVKIKCLIKVAVGRWGGRILKLY
GFKSSVNGTYNLPAVSDNISESENITIQIQKQNKEVKRYKTIKKLKIEIK
NNKGWEAMKLNLQLGIH*

protein sequence of SMED30019420-orf-3

>SMED30019420-orf-3 ID=SMED30019420-orf-3|Name=SMED30019420-orf-3|organism=Schmidtea mediterranea sexual|type=polypeptide|length=217bp
MKPRIEVSKVNRFTCKIKRNEVTTGESVKDNEVENANNENLSGEEICIGA
KQQNVQIFIKASGETITQDVELQWPMSKIKKIIHQRGEMSYESQKLYRNG
KQLNDNKTSSDNNITSGSTIFLLYNQLGGTETEEISGRKKRQSQKWRLTR
KRNILLAAISTNDCLKLAEESEGKFYKMNESFIEFLDRNKKVGKKEVKEI
LEKDGSEIYQKKEKVD*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000035	Category 3 cell
PLANA:0000421	tail
PLANA:0002032	epidermal cell
PLANA:0002075	pigment cell
PLANA:0002089	reproductive organ

Vocabulary: **INTERPRO**
Term	Definition
IPR000626	Ubiquitin-like_dom
IPR029071	Ubiquitin-like_domsf

Vocabulary: **molecular function**
Term	Definition
GO:0005515	protein binding

Main menu

SMED30019420

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 6

Owlery

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