Dmd-3

Overview


Name	Dmd-3 (AGL61629.1)
Smed ID	SMED30019413
Length (bp)	959

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of Dmd-3 (SMED30019413) t-SNE clustered cells

Violin plots show distribution of expression levels for Dmd-3 (SMED30019413) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Dmd-3 (SMED30019413) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Dmd-3 (SMED30019413) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30019413

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 6

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
parapharyngeal region	SMED30019413	h1SMcG0022046	SmedASXL_005666	SmedAsxl_ww_GCZZ01	PMID:27034770 Currie et al., 2016
Smed sexual biotype	SMED30019413	h1SMcG0022046	Contig50439	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30019413	h1SMcG0022046	Contig50439	newmark_ests	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30019413	h1SMcG0022046	Contig47334	newmark_ests	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30019413	h1SMcG0022046	Contig47334	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
muscle cell	SMED30019413	h1SMcG0022046	dd_Smed_v4_13283_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018

Note: Hover over icons to view figure legend

Homology

[Close]

BLAST of Dmd-3 vs. Ensembl Human
Match: DMRT2 (doublesex and mab-3 related transcription factor 2 [Source:HGNC Symbol;Acc:HGNC:2935])

HSP 1 Score: 65.0846 bits (157), Expect = 2.694e-23
Identity = 27/29 (93.10%), Postives = 28/29 (96.55%), Query Frame = 3

Query:  177 VSRTPKCARCRNHGVVSCLKGHKRFCHWR 263
            +SRTPKCARCRNHGVVSCLKGHKRFC WR
Sbjct:  117 LSRTPKCARCRNHGVVSCLKGHKRFCRWR 145

HSP 2 Score: 63.1586 bits (152), Expect = 2.694e-23
Identity = 42/114 (36.84%), Postives = 54/114 (47.37%), Query Frame = 1

Query:  262 DCHCSNCLLVIERQRVMAAQVALRRQQTTSACKLPTNTKT---EKSSYKRLIRQSISTVKSKKSHTIFRKQSEKKESNMKNNSSGSVESILERLPVDLGTQFTERLRKRKAFAD 594
            DC C+NCLLV+ERQRVMAAQVALRRQQ T   K  +  +     K+ Y+R +R      KS     I               +              L    ++R+RKR+AFAD
Sbjct:  146 DCQCANCLLVVERQRVMAAQVALRRQQATEDKKGLSGKQNNFERKAVYQRQVRAPSLLAKS-----ILEGYRPIPAETYVGGT------------FPLPPPVSDRMRKRRAFAD 242

[Close]

Query:  177 VSRTPKCARCRNHGVVSCLKGHKRFCHWR 263
            +SRTPKCARCRNHGVVSCLKGHKRFC WR
Sbjct:  117 LSRTPKCARCRNHGVVSCLKGHKRFCRWR 145

HSP 2 Score: 63.1586 bits (152), Expect = 2.694e-23
Identity = 42/114 (36.84%), Postives = 54/114 (47.37%), Query Frame = 1

Query:  262 DCHCSNCLLVIERQRVMAAQVALRRQQTTSACKLPTNTKT---EKSSYKRLIRQSISTVKSKKSHTIFRKQSEKKESNMKNNSSGSVESILERLPVDLGTQFTERLRKRKAFAD 594
            DC C+NCLLV+ERQRVMAAQVALRRQQ T   K  +  +     K+ Y+R +R      KS     I               +              L    ++R+RKR+AFAD
Sbjct:  146 DCQCANCLLVVERQRVMAAQVALRRQQATEDKKGLSGKQNNFERKAVYQRQVRAPSLLAKS-----ILEGYRPIPAETYVGGT------------FPLPPPVSDRMRKRRAFAD 242

[Close]

BLAST of Dmd-3 vs. Ensembl Human
Match: DMRT2 (doublesex and mab-3 related transcription factor 2 [Source:HGNC Symbol;Acc:HGNC:2935])

HSP 1 Score: 63.5438 bits (153), Expect = 4.648e-22
Identity = 27/29 (93.10%), Postives = 28/29 (96.55%), Query Frame = 3

Query:  177 VSRTPKCARCRNHGVVSCLKGHKRFCHWR 263
            +SRTPKCARCRNHGVVSCLKGHKRFC WR
Sbjct:  117 LSRTPKCARCRNHGVVSCLKGHKRFCRWR 145

HSP 2 Score: 60.4622 bits (145), Expect = 4.648e-22
Identity = 25/29 (86.21%), Postives = 27/29 (93.10%), Query Frame = 1

Query:  262 DCHCSNCLLVIERQRVMAAQVALRRQQTT 348
            DC C+NCLLV+ERQRVMAAQVALRRQQ T
Sbjct:  146 DCQCANCLLVVERQRVMAAQVALRRQQAT 174

[Close]

Query:  177 VSRTPKCARCRNHGVVSCLKGHKRFCHWR 263
            +SRTPKCARCRNHGVVSCLKGHKRFC WR
Sbjct:  117 LSRTPKCARCRNHGVVSCLKGHKRFCRWR 145

HSP 2 Score: 60.4622 bits (145), Expect = 4.648e-22
Identity = 25/29 (86.21%), Postives = 27/29 (93.10%), Query Frame = 1

Query:  262 DCHCSNCLLVIERQRVMAAQVALRRQQTT 348
            DC C+NCLLV+ERQRVMAAQVALRRQQ T
Sbjct:  146 DCQCANCLLVVERQRVMAAQVALRRQQAT 174

[Close]

Query:  177 VSRTPKCARCRNHGVVSCLKGHKRFCHWR 263
            +SRTPKCARCRNHGVVSCLKGHKRFC WR
Sbjct:  117 LSRTPKCARCRNHGVVSCLKGHKRFCRWR 145

HSP 2 Score: 60.4622 bits (145), Expect = 4.648e-22
Identity = 25/29 (86.21%), Postives = 27/29 (93.10%), Query Frame = 1

Query:  262 DCHCSNCLLVIERQRVMAAQVALRRQQTT 348
            DC C+NCLLV+ERQRVMAAQVALRRQQ T
Sbjct:  146 DCQCANCLLVVERQRVMAAQVALRRQQAT 174

[Close]

BLAST of Dmd-3 vs. Ensembl Celegans
Match: dmd-5 (DM (Doublesex/MAB-3) Domain family [Source:UniProtKB/TrEMBL;Acc:Q19291])

HSP 1 Score: 55.4546 bits (132), Expect = 1.091e-17
Identity = 21/27 (77.78%), Postives = 23/27 (85.19%), Query Frame = 3

Query:  183 RTPKCARCRNHGVVSCLKGHKRFCHWR 263
            RTPKCARCRNHG  S LKGHKR+C W+
Sbjct:   29 RTPKCARCRNHGTTSALKGHKRYCQWK 55

HSP 2 Score: 52.7582 bits (125), Expect = 1.091e-17
Identity = 21/28 (75.00%), Postives = 24/28 (85.71%), Query Frame = 1

Query:  262 DCHCSNCLLVIERQRVMAAQVALRRQQT 345
            DC C+ C L+ ERQRVMAAQVALRRQQ+
Sbjct:   56 DCMCAKCTLIAERQRVMAAQVALRRQQS 83

[Close]

BLAST of Dmd-3 vs. Ensembl Celegans
Match: dmd-4 (DM (Doublesex/MAB-3) Domain family [Source:UniProtKB/TrEMBL;Acc:Q18248])

HSP 1 Score: 47.3654 bits (111), Expect = 4.566e-13
Identity = 20/28 (71.43%), Postives = 23/28 (82.14%), Query Frame = 3

Query:  180 SRTPKCARCRNHGVVSCLKGHKRFCHWR 263
             R PKCARCRNHG+VS LKGHKR C ++
Sbjct:   17 ERKPKCARCRNHGLVSWLKGHKRHCKYK 44

HSP 2 Score: 45.4394 bits (106), Expect = 4.566e-13
Identity = 19/29 (65.52%), Postives = 23/29 (79.31%), Query Frame = 1

Query:  262 DCHCSNCLLVIERQRVMAAQVALRRQQTT 348
            +C C  C L+ ERQRVMAAQVAL+R+Q T
Sbjct:   45 ECACEKCNLIAERQRVMAAQVALKRRQAT 73

[Close]

BLAST of Dmd-3 vs. Ensembl Celegans
Match: dmd-3 (DM (Doublesex/MAB-3) Domain family [Source:UniProtKB/TrEMBL;Acc:Q9XWN9])

HSP 1 Score: 39.2762 bits (90), Expect = 2.691e-8
Identity = 15/27 (55.56%), Postives = 22/27 (81.48%), Query Frame = 1

Query:  262 DCHCSNCLLVIERQRVMAAQVALRRQQ 342
            DC C+ C +V+ERQ++MA Q+ LRR+Q
Sbjct:   46 DCFCAKCQVVVERQKLMADQIKLRRRQ 72

HSP 2 Score: 37.3502 bits (85), Expect = 2.691e-8
Identity = 17/35 (48.57%), Postives = 22/35 (62.86%), Query Frame = 3

Query:  159 AMKLYNVSRTPKCARCRNHGVVSCLKGHKRFCHWR 263
            ++K+    R P C RC  H VV+ LKGHKR C +R
Sbjct:   11 SVKVDRDERRPNCQRCAQHSVVNRLKGHKRACPFR 45

[Close]

BLAST of Dmd-3 vs. Ensembl Celegans
Match: dmd-3 (DM (Doublesex/MAB-3) Domain family [Source:UniProtKB/TrEMBL;Acc:Q9XWN9])

HSP 1 Score: 39.2762 bits (90), Expect = 5.417e-8
Identity = 15/27 (55.56%), Postives = 22/27 (81.48%), Query Frame = 1

Query:  262 DCHCSNCLLVIERQRVMAAQVALRRQQ 342
            DC C+ C +V+ERQ++MA Q+ LRR+Q
Sbjct:  140 DCFCAKCQVVVERQKLMADQIKLRRRQ 166

HSP 2 Score: 35.8094 bits (81), Expect = 5.417e-8
Identity = 16/28 (57.14%), Postives = 18/28 (64.29%), Query Frame = 3

Query:  180 SRTPKCARCRNHGVVSCLKGHKRFCHWR 263
             R P C RC  H VV+ LKGHKR C +R
Sbjct:  112 ERRPNCQRCAQHSVVNRLKGHKRACPFR 139

[Close]

BLAST of Dmd-3 vs. Ensembl Celegans
Match: dmd-10 (DM (Doublesex/MAB-3) Domain family [Source:UniProtKB/TrEMBL;Acc:G5EEJ1])

HSP 1 Score: 36.965 bits (84), Expect = 7.645e-7
Identity = 13/27 (48.15%), Postives = 21/27 (77.78%), Query Frame = 1

Query:  262 DCHCSNCLLVIERQRVMAAQVALRRQQ 342
            +C C+ C +V ERQ++MA Q+ +RR+Q
Sbjct:   63 ECPCAKCAVVSERQKLMADQIKIRRRQ 89

HSP 2 Score: 34.2686 bits (77), Expect = 7.645e-7
Identity = 15/27 (55.56%), Postives = 17/27 (62.96%), Query Frame = 3

Query:  183 RTPKCARCRNHGVVSCLKGHKRFCHWR 263
            R P C +C  HG  S LKGHKR C +R
Sbjct:   36 RVPNCQKCGQHGRKSRLKGHKRSCPFR 62

[Close]

BLAST of Dmd-3 vs. Ensembl Fly
Match: dmrt11E (gene:FBgn0030477 transcript:FBtr0073768)

HSP 1 Score: 58.5362 bits (140), Expect = 1.447e-18
Identity = 23/27 (85.19%), Postives = 25/27 (92.59%), Query Frame = 3

Query:  183 RTPKCARCRNHGVVSCLKGHKRFCHWR 263
            RTPKCARCRNHGV+SC+KGHKR C WR
Sbjct:  118 RTPKCARCRNHGVISCVKGHKRLCRWR 144

HSP 2 Score: 53.1434 bits (126), Expect = 1.447e-18
Identity = 23/31 (74.19%), Postives = 26/31 (83.87%), Query Frame = 1

Query:  262 DCHCSNCLLVIERQRVMAAQVALRRQQTTSA 354
            +C C NC LV++RQRVMAAQVALRRQQT  A
Sbjct:  145 ECCCPNCQLVVDRQRVMAAQVALRRQQTMEA 175

[Close]

BLAST of Dmd-3 vs. Ensembl Fly
Match: dmrt99B (gene:FBgn0039683 transcript:FBtr0085454)

HSP 1 Score: 59.3066 bits (142), Expect = 1.573e-18
Identity = 24/27 (88.89%), Postives = 25/27 (92.59%), Query Frame = 3

Query:  183 RTPKCARCRNHGVVSCLKGHKRFCHWR 263
            RTPKCARCRNHGVVS LKGHKR+C WR
Sbjct:   37 RTPKCARCRNHGVVSALKGHKRYCRWR 63

HSP 2 Score: 51.9878 bits (123), Expect = 1.573e-18
Identity = 21/28 (75.00%), Postives = 23/28 (82.14%), Query Frame = 1

Query:  262 DCHCSNCLLVIERQRVMAAQVALRRQQT 345
            DC C+ C L+ ERQRVMAAQVALRRQQ 
Sbjct:   64 DCVCAKCTLIAERQRVMAAQVALRRQQA 91

[Close]

BLAST of Dmd-3 vs. Ensembl Fly
Match: dmrt93B (gene:FBgn0038851 transcript:FBtr0084007)

HSP 1 Score: 48.1358 bits (113), Expect = 5.969e-14
Identity = 18/29 (62.07%), Postives = 24/29 (82.76%), Query Frame = 1

Query:  262 DCHCSNCLLVIERQRVMAAQVALRRQQTT 348
            +C C+ C+L+ ERQR+MAAQVAL+RQQ  
Sbjct:   66 NCKCAKCVLIFERQRIMAAQVALKRQQAV 94

HSP 2 Score: 47.7506 bits (112), Expect = 5.969e-14
Identity = 17/27 (62.96%), Postives = 23/27 (85.19%), Query Frame = 3

Query:  183 RTPKCARCRNHGVVSCLKGHKRFCHWR 263
            R PKCARCRNHG++S L+GHK+ C ++
Sbjct:   39 RVPKCARCRNHGIISELRGHKKLCTYK 65

[Close]

BLAST of Dmd-3 vs. Ensembl Fly
Match: dsx (gene:FBgn0000504 transcript:FBtr0330073)

HSP 1 Score: 42.743 bits (99), Expect = 1.314e-9
Identity = 17/25 (68.00%), Postives = 20/25 (80.00%), Query Frame = 3

Query:  189 PKCARCRNHGVVSCLKGHKRFCHWR 263
            P CARCRNHG+   LKGHKR+C +R
Sbjct:   42 PNCARCRNHGLKITLKGHKRYCKFR 66

HSP 2 Score: 38.5058 bits (88), Expect = 1.314e-9
Identity = 16/26 (61.54%), Postives = 17/26 (65.38%), Query Frame = 1

Query:  265 CHCSNCLLVIERQRVMAAQVALRRQQ 342
            C C  C L  +RQRVMA Q ALRR Q
Sbjct:   68 CTCEKCRLTADRQRVMALQTALRRAQ 93

[Close]

BLAST of Dmd-3 vs. Ensembl Fly
Match: dsx (gene:FBgn0000504 transcript:FBtr0081759)

HSP 1 Score: 42.743 bits (99), Expect = 1.314e-9
Identity = 17/25 (68.00%), Postives = 20/25 (80.00%), Query Frame = 3

Query:  189 PKCARCRNHGVVSCLKGHKRFCHWR 263
            P CARCRNHG+   LKGHKR+C +R
Sbjct:   42 PNCARCRNHGLKITLKGHKRYCKFR 66

HSP 2 Score: 38.5058 bits (88), Expect = 1.314e-9
Identity = 16/26 (61.54%), Postives = 17/26 (65.38%), Query Frame = 1

Query:  265 CHCSNCLLVIERQRVMAAQVALRRQQ 342
            C C  C L  +RQRVMA Q ALRR Q
Sbjct:   68 CTCEKCRLTADRQRVMALQTALRRAQ 93

[Close]

BLAST of Dmd-3 vs. Ensembl Zebrafish
Match: dmrt2a (doublesex and mab-3 related transcription factor 2a [Source:ZFIN;Acc:ZDB-GENE-990621-7])

HSP 1 Score: 68.9366 bits (167), Expect = 2.917e-25
Identity = 46/114 (40.35%), Postives = 61/114 (53.51%), Query Frame = 1

Query:  262 DCHCSNCLLVIERQRVMAAQVALRRQQTTSACKLPTNTKTE---KSSYKRLIRQSISTVKSKKSHTIFRKQSEKKESNMKNNSSGSVESILERLPVDLGTQFTERLRKRKAFAD 594
            DC C+NCLLV+ERQRVMAAQVALRRQQ T   K  T  +     ++ Y+R IR S          T+  K   +    ++N+        L   P  L    ++R+RKR+AFAD
Sbjct:   84 DCQCANCLLVVERQRVMAAQVALRRQQATEDKKGITGKQIPVERRNIYQRHIRPS----------TMLAKSILEGYRPVQNDP------FLGANPA-LPPPLSDRMRKRRAFAD 180

HSP 2 Score: 65.855 bits (159), Expect = 2.917e-25
Identity = 31/54 (57.41%), Postives = 35/54 (64.81%), Query Frame = 3

Query:  102 GEFISNFKPEFSQRYRNYFAMKLYNVSRTPKCARCRNHGVVSCLKGHKRFCHWR 263
            GE  +  K +      N  +     +SRTPKCARCRNHGVVSCLKGHKRFC WR
Sbjct:   30 GEDDTGSKDDDKSDRLNLGSGDQRKLSRTPKCARCRNHGVVSCLKGHKRFCRWR 83

[Close]

Query:  262 DCHCSNCLLVIERQRVMAAQVALRRQQTTSACKLPTNTKTE---KSSYKRLIRQSISTVKSKKSHTIFRKQSEKKESNMKNNSSGSVESILERLPVDLGTQFTERLRKRKAFAD 594
            DC C+NCLLV+ERQRVMAAQVALRRQQ T   K  T  +     ++ Y+R IR S          T+  K   +    ++N+        L   P  L    ++R+RKR+AFAD
Sbjct:   84 DCQCANCLLVVERQRVMAAQVALRRQQATEDKKGITGKQIPVERRNIYQRHIRPS----------TMLAKSILEGYRPVQNDP------FLGANPA-LPPPLSDRMRKRRAFAD 180

HSP 2 Score: 65.855 bits (159), Expect = 2.917e-25
Identity = 31/54 (57.41%), Postives = 35/54 (64.81%), Query Frame = 3

Query:  102 GEFISNFKPEFSQRYRNYFAMKLYNVSRTPKCARCRNHGVVSCLKGHKRFCHWR 263
            GE  +  K +      N  +     +SRTPKCARCRNHGVVSCLKGHKRFC WR
Sbjct:   30 GEDDTGSKDDDKSDRLNLGSGDQRKLSRTPKCARCRNHGVVSCLKGHKRFCRWR 83

[Close]

Query:  262 DCHCSNCLLVIERQRVMAAQVALRRQQTTSACKLPTNTKTE---KSSYKRLIRQSISTVKSKKSHTIFRKQSEKKESNMKNNSSGSVESILERLPVDLGTQFTERLRKRKAFAD 594
            DC C+NCLLV+ERQRVMAAQVALRRQQ T   K  T  +     ++ Y+R IR S          T+  K   +    ++N+        L   P  L    ++R+RKR+AFAD
Sbjct:   84 DCQCANCLLVVERQRVMAAQVALRRQQATEDKKGITGKQIPVERRNIYQRHIRPS----------TMLAKSILEGYRPVQNDP------FLGANPA-LPPPLSDRMRKRRAFAD 180

HSP 2 Score: 65.855 bits (159), Expect = 2.917e-25
Identity = 31/54 (57.41%), Postives = 35/54 (64.81%), Query Frame = 3

Query:  102 GEFISNFKPEFSQRYRNYFAMKLYNVSRTPKCARCRNHGVVSCLKGHKRFCHWR 263
            GE  +  K +      N  +     +SRTPKCARCRNHGVVSCLKGHKRFC WR
Sbjct:   30 GEDDTGSKDDDKSDRLNLGSGDQRKLSRTPKCARCRNHGVVSCLKGHKRFCRWR 83

[Close]

BLAST of Dmd-3 vs. Ensembl Zebrafish
Match: dmrt2b (doublesex and mab-3 related transcription factor 2b [Source:ZFIN;Acc:ZDB-GENE-080813-1])

HSP 1 Score: 67.0106 bits (162), Expect = 4.219e-22
Identity = 46/114 (40.35%), Postives = 58/114 (50.88%), Query Frame = 1

Query:  262 DCHCSNCLLVIERQRVMAAQVALRRQQTTSACKLPTNTKT-EKSSYKRLIRQSISTVKSKKSHTIFRKQSEKKESNMKNNSSGSVESILERLPVDLG-TQFTERLRKRKAFADE 597
            DC C+NCLLV+ERQRVMAAQVALRRQQ T   K   N+    +++Y+R  R      KS     I                 G    +LE  P  L     + R+RKR+AFAD+
Sbjct:   73 DCQCANCLLVVERQRVMAAQVALRRQQATEGKKGQKNSAVLRRTAYQRYTRAPSLLAKS-----IL---------------EGYKPPVLEDWPKRLHQPPVSVRMRKRRAFADK 166

HSP 2 Score: 56.9954 bits (136), Expect = 4.219e-22
Identity = 24/29 (82.76%), Postives = 26/29 (89.66%), Query Frame = 3

Query:  177 VSRTPKCARCRNHGVVSCLKGHKRFCHWR 263
            +SR+PKCARCRNHGVVS LKGHKR C WR
Sbjct:   44 LSRSPKCARCRNHGVVSRLKGHKRLCRWR 72

[Close]

Query:  262 DCHCSNCLLVIERQRVMAAQVALRRQQTTSACKLPTNTKT-EKSSYKRLIRQSISTVKSKKSHTIFRKQSEKKESNMKNNSSGSVESILERLPVDLG-TQFTERLRKRKAFADE 597
            DC C+NCLLV+ERQRVMAAQVALRRQQ T   K   N+    +++Y+R  R      KS     I                 G    +LE  P  L     + R+RKR+AFAD+
Sbjct:   73 DCQCANCLLVVERQRVMAAQVALRRQQATEGKKGQKNSAVLRRTAYQRYTRAPSLLAKS-----IL---------------EGYKPPVLEDWPKRLHQPPVSVRMRKRRAFADK 166

HSP 2 Score: 56.9954 bits (136), Expect = 4.219e-22
Identity = 24/29 (82.76%), Postives = 26/29 (89.66%), Query Frame = 3

Query:  177 VSRTPKCARCRNHGVVSCLKGHKRFCHWR 263
            +SR+PKCARCRNHGVVS LKGHKR C WR
Sbjct:   44 LSRSPKCARCRNHGVVSRLKGHKRLCRWR 72

[Close]

BLAST of Dmd-3 vs. Ensembl Xenopus
Match: kdm2b (lysine (K)-specific demethylase 2B [Source:Xenbase;Acc:XB-GENE-990506])

HSP 1 Score: 70.0922 bits (170), Expect = 2.485e-25
Identity = 45/114 (39.47%), Postives = 56/114 (49.12%), Query Frame = 1

Query:  262 DCHCSNCLLVIERQRVMAAQVALRRQQTTS---ACKLPTNTKTEKSSYKRLIRQSISTVKSKKSHTIFRKQSEKKESNMKNNSSGSVESILERLPVDLGTQFTERLRKRKAFAD 594
            DC C+NCLLV+ERQRVMAAQVALRRQQ T          +    K  Y+R IR      KS                 ++       +S L   P  L  Q ++R+RKR+AFAD
Sbjct:  120 DCQCANCLLVVERQRVMAAQVALRRQQATEDKKGLSAKPSIAERKPVYQRHIRPPSLLAKSI----------------LEGYRPVQTDSYLGTSP-PLPAQVSDRMRKRRAFAD 216

HSP 2 Score: 65.0846 bits (157), Expect = 2.485e-25
Identity = 27/29 (93.10%), Postives = 28/29 (96.55%), Query Frame = 3

Query:  177 VSRTPKCARCRNHGVVSCLKGHKRFCHWR 263
            +SRTPKCARCRNHGVVSCLKGHKRFC WR
Sbjct:   91 LSRTPKCARCRNHGVVSCLKGHKRFCRWR 119

[Close]

BLAST of Dmd-3 vs. Ensembl Xenopus
Match: dmrta1 (DMRT like family A1 [Source:NCBI gene;Acc:779901])

HSP 1 Score: 59.6918 bits (143), Expect = 3.351e-19
Identity = 25/27 (92.59%), Postives = 25/27 (92.59%), Query Frame = 3

Query:  183 RTPKCARCRNHGVVSCLKGHKRFCHWR 263
            RTPKCARCRNHGVVS LKGHKRFC WR
Sbjct:   79 RTPKCARCRNHGVVSALKGHKRFCRWR 105

HSP 2 Score: 54.6842 bits (130), Expect = 3.351e-19
Identity = 22/34 (64.71%), Postives = 26/34 (76.47%), Query Frame = 1

Query:  262 DCHCSNCLLVIERQRVMAAQVALRRQQTTSACKL 363
            DC C+ C L+ ERQRVMAAQVALRRQQ    C++
Sbjct:  106 DCSCAKCTLIAERQRVMAAQVALRRQQAQEECEV 139

[Close]

BLAST of Dmd-3 vs. Ensembl Xenopus
Match: dynll1 (dynein light chain LC8-type 1 [Source:Xenbase;Acc:XB-GENE-854521])

HSP 1 Score: 58.5362 bits (140), Expect = 2.021e-18
Identity = 23/27 (85.19%), Postives = 25/27 (92.59%), Query Frame = 3

Query:  183 RTPKCARCRNHGVVSCLKGHKRFCHWR 263
            RTPKCARCRNHGVVS LKGHKR+C W+
Sbjct:   45 RTPKCARCRNHGVVSALKGHKRYCRWK 71

HSP 2 Score: 53.5286 bits (127), Expect = 2.021e-18
Identity = 21/28 (75.00%), Postives = 23/28 (82.14%), Query Frame = 1

Query:  262 DCHCSNCLLVIERQRVMAAQVALRRQQT 345
            DC C+ C L+ ERQRVMAAQVALRRQQ 
Sbjct:   72 DCMCAKCTLIAERQRVMAAQVALRRQQA 99

[Close]

BLAST of Dmd-3 vs. Ensembl Xenopus
Match: st3gal4 (ST3 beta-galactoside alpha-2,3-sialyltransferase 4 [Source:Xenbase;Acc:XB-GENE-963280])

HSP 1 Score: 55.0694 bits (131), Expect = 7.135e-17
Identity = 22/28 (78.57%), Postives = 24/28 (85.71%), Query Frame = 1

Query:  262 DCHCSNCLLVIERQRVMAAQVALRRQQT 345
            DC C  C+L+IERQRVMAAQVALRRQQ 
Sbjct:   51 DCTCEKCILIIERQRVMAAQVALRRQQA 78

HSP 2 Score: 51.6026 bits (122), Expect = 7.135e-17
Identity = 21/29 (72.41%), Postives = 26/29 (89.66%), Query Frame = 3

Query:  177 VSRTPKCARCRNHGVVSCLKGHKRFCHWR 263
            + RTPKCARCRNHGV+S LKGHKR+C ++
Sbjct:   22 LQRTPKCARCRNHGVLSWLKGHKRYCRFK 50

[Close]

BLAST of Dmd-3 vs. Ensembl Xenopus
Match: DMRT1 (doublesex and mab-3 related transcription factor 1 [Source:NCBI gene;Acc:100488257])

HSP 1 Score: 53.1434 bits (126), Expect = 2.845e-16
Identity = 22/27 (81.48%), Postives = 22/27 (81.48%), Query Frame = 3

Query:  183 RTPKCARCRNHGVVSCLKGHKRFCHWR 263
            R PKCARCRNHG  S LKGHKRFC WR
Sbjct:   28 RLPKCARCRNHGYASPLKGHKRFCMWR 54

HSP 2 Score: 51.9878 bits (123), Expect = 2.845e-16
Identity = 21/28 (75.00%), Postives = 22/28 (78.57%), Query Frame = 1

Query:  262 DCHCSNCLLVIERQRVMAAQVALRRQQT 345
            DC C  C L+ ERQRVMAAQVALRRQQ 
Sbjct:   55 DCQCKKCSLIAERQRVMAAQVALRRQQA 82

[Close]

BLAST of Dmd-3 vs. Ensembl Mouse
Match: Dmrt2 (doublesex and mab-3 related transcription factor 2 [Source:MGI Symbol;Acc:MGI:1330307])

HSP 1 Score: 66.2402 bits (160), Expect = 2.304e-24
Identity = 44/114 (38.60%), Postives = 62/114 (54.39%), Query Frame = 1

Query:  262 DCHCSNCLLVIERQRVMAAQVALRRQQTTSACKLPT---NTKTEKSSYKRLIRQSISTVKSKKSHTIFRKQSEKKESNMKNNSSGSVESILERLPVDLGTQFTERLRKRKAFAD 594
            DC C+NCLLV+ERQRVMAAQVALRRQQ T   K  +   N    K+ Y+R +R          + ++  K      S ++     + E+ L    + L    ++R+RKR+AFAD
Sbjct:  146 DCQCANCLLVVERQRVMAAQVALRRQQATEDKKGLSGKQNNFDRKAVYQRQVR----------APSLLAK------SILEGYRPMTAETYLGGT-LPLPPPVSDRMRKRRAFAD 242

HSP 2 Score: 65.0846 bits (157), Expect = 2.304e-24
Identity = 27/29 (93.10%), Postives = 28/29 (96.55%), Query Frame = 3

Query:  177 VSRTPKCARCRNHGVVSCLKGHKRFCHWR 263
            +SRTPKCARCRNHGVVSCLKGHKRFC WR
Sbjct:  117 LSRTPKCARCRNHGVVSCLKGHKRFCRWR 145

[Close]

BLAST of Dmd-3 vs. Ensembl Mouse
Match: Dmrta1 (doublesex and mab-3 related transcription factor like family A1 [Source:MGI Symbol;Acc:MGI:2653627])

HSP 1 Score: 59.6918 bits (143), Expect = 3.147e-19
Identity = 25/27 (92.59%), Postives = 25/27 (92.59%), Query Frame = 3

Query:  183 RTPKCARCRNHGVVSCLKGHKRFCHWR 263
            RTPKCARCRNHGVVS LKGHKRFC WR
Sbjct:   82 RTPKCARCRNHGVVSALKGHKRFCRWR 108

HSP 2 Score: 54.6842 bits (130), Expect = 3.147e-19
Identity = 29/71 (40.85%), Postives = 38/71 (53.52%), Query Frame = 1

Query:  262 DCHCSNCLLVIERQRVMAAQVALRRQQTTSACKLPTNTKTEKSSYKRLIRQSISTVKSKKSHTIFRKQSEK 474
            DC C+ C L+ ERQRVMAAQVALRRQQ           ++E     RL+ Q  S   ++ S    R +S +
Sbjct:  109 DCACAKCTLIAERQRVMAAQVALRRQQ--------AQEESEARGLHRLLYQGSSGSGAQASGGSGRTESPQ 171

[Close]

BLAST of Dmd-3 vs. Ensembl Mouse
Match: Dmrta2 (doublesex and mab-3 related transcription factor like family A2 [Source:MGI Symbol;Acc:MGI:2653629])

HSP 1 Score: 57.7658 bits (138), Expect = 3.926e-18
Identity = 23/27 (85.19%), Postives = 25/27 (92.59%), Query Frame = 3

Query:  183 RTPKCARCRNHGVVSCLKGHKRFCHWR 263
            RTPKCARCRNHGVVS LKGHKR+C W+
Sbjct:   65 RTPKCARCRNHGVVSALKGHKRYCRWK 91

HSP 2 Score: 52.7582 bits (125), Expect = 3.926e-18
Identity = 21/28 (75.00%), Postives = 23/28 (82.14%), Query Frame = 1

Query:  262 DCHCSNCLLVIERQRVMAAQVALRRQQT 345
            DC C+ C L+ ERQRVMAAQVALRRQQ 
Sbjct:   92 DCLCAKCTLIAERQRVMAAQVALRRQQA 119

[Close]

BLAST of Dmd-3 vs. Ensembl Mouse
Match: Dmrt3 (doublesex and mab-3 related transcription factor 3 [Source:MGI Symbol;Acc:MGI:2449470])

HSP 1 Score: 55.4546 bits (132), Expect = 3.685e-17
Identity = 22/28 (78.57%), Postives = 24/28 (85.71%), Query Frame = 1

Query:  262 DCHCSNCLLVIERQRVMAAQVALRRQQT 345
            DC C  C+L+IERQRVMAAQVALRRQQ 
Sbjct:   52 DCTCEKCILIIERQRVMAAQVALRRQQA 79

HSP 2 Score: 51.9878 bits (123), Expect = 3.685e-17
Identity = 21/29 (72.41%), Postives = 26/29 (89.66%), Query Frame = 3

Query:  177 VSRTPKCARCRNHGVVSCLKGHKRFCHWR 263
            + RTPKCARCRNHGV+S LKGHKR+C ++
Sbjct:   23 LQRTPKCARCRNHGVLSWLKGHKRYCRFK 51

[Close]

BLAST of Dmd-3 vs. Ensembl Mouse
Match: Dmrt1 (doublesex and mab-3 related transcription factor 1 [Source:MGI Symbol;Acc:MGI:1354733])

HSP 1 Score: 53.5286 bits (127), Expect = 1.049e-16
Identity = 22/27 (81.48%), Postives = 22/27 (81.48%), Query Frame = 3

Query:  183 RTPKCARCRNHGVVSCLKGHKRFCHWR 263
            R PKCARCRNHG  S LKGHKRFC WR
Sbjct:   70 RLPKCARCRNHGYASPLKGHKRFCMWR 96

HSP 2 Score: 51.9878 bits (123), Expect = 1.049e-16
Identity = 21/28 (75.00%), Postives = 22/28 (78.57%), Query Frame = 1

Query:  262 DCHCSNCLLVIERQRVMAAQVALRRQQT 345
            DC C  C L+ ERQRVMAAQVALRRQQ 
Sbjct:   97 DCQCKKCSLIAERQRVMAAQVALRRQQA 124

[Close]

BLAST of Dmd-3 vs. UniProt/SwissProt
Match: sp|Q8BG36|DMRT2_MOUSE (Doublesex- and mab-3-related transcription factor 2 OS=Mus musculus OX=10090 GN=Dmrt2 PE=1 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 1.355e-23
Identity = 44/114 (38.60%), Postives = 62/114 (54.39%), Query Frame = 1

Query:  262 DCHCSNCLLVIERQRVMAAQVALRRQQTTSACKLPT---NTKTEKSSYKRLIRQSISTVKSKKSHTIFRKQSEKKESNMKNNSSGSVESILERLPVDLGTQFTERLRKRKAFAD 594
            DC C+NCLLV+ERQRVMAAQVALRRQQ T   K  +   N    K+ Y+R +R          + ++  K      S ++     + E+ L    + L    ++R+RKR+AFAD
Sbjct:  146 DCQCANCLLVVERQRVMAAQVALRRQQATEDKKGLSGKQNNFDRKAVYQRQVR----------APSLLAK------SILEGYRPMTAETYLGGT-LPLPPPVSDRMRKRRAFAD 242

HSP 2 Score: 65.0846 bits (157), Expect = 1.355e-23
Identity = 27/29 (93.10%), Postives = 28/29 (96.55%), Query Frame = 3

Query:  177 VSRTPKCARCRNHGVVSCLKGHKRFCHWR 263
            +SRTPKCARCRNHGVVSCLKGHKRFC WR
Sbjct:  117 LSRTPKCARCRNHGVVSCLKGHKRFCRWR 145

[Close]

BLAST of Dmd-3 vs. UniProt/SwissProt
Match: sp|Q9Y5R5|DMRT2_HUMAN (Doublesex- and mab-3-related transcription factor 2 OS=Homo sapiens OX=9606 GN=DMRT2 PE=1 SV=2)

HSP 1 Score: 65.0846 bits (157), Expect = 1.150e-22
Identity = 27/29 (93.10%), Postives = 28/29 (96.55%), Query Frame = 3

Query:  177 VSRTPKCARCRNHGVVSCLKGHKRFCHWR 263
            +SRTPKCARCRNHGVVSCLKGHKRFC WR
Sbjct:  117 LSRTPKCARCRNHGVVSCLKGHKRFCRWR 145

HSP 2 Score: 63.1586 bits (152), Expect = 1.150e-22
Identity = 42/114 (36.84%), Postives = 54/114 (47.37%), Query Frame = 1

Query:  262 DCHCSNCLLVIERQRVMAAQVALRRQQTTSACKLPTNTKT---EKSSYKRLIRQSISTVKSKKSHTIFRKQSEKKESNMKNNSSGSVESILERLPVDLGTQFTERLRKRKAFAD 594
            DC C+NCLLV+ERQRVMAAQVALRRQQ T   K  +  +     K+ Y+R +R      KS     I               +              L    ++R+RKR+AFAD
Sbjct:  146 DCQCANCLLVVERQRVMAAQVALRRQQATEDKKGLSGKQNNFERKAVYQRQVRAPSLLAKS-----ILEGYRPIPAETYVGGT------------FPLPPPVSDRMRKRRAFAD 242

[Close]

BLAST of Dmd-3 vs. UniProt/SwissProt
Match: sp|Q8CFG4|DMRTA_MOUSE (Doublesex- and mab-3-related transcription factor A1 OS=Mus musculus OX=10090 GN=Dmrta1 PE=2 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 1.851e-18
Identity = 25/27 (92.59%), Postives = 25/27 (92.59%), Query Frame = 3

Query:  183 RTPKCARCRNHGVVSCLKGHKRFCHWR 263
            RTPKCARCRNHGVVS LKGHKRFC WR
Sbjct:   82 RTPKCARCRNHGVVSALKGHKRFCRWR 108

HSP 2 Score: 54.6842 bits (130), Expect = 1.851e-18
Identity = 29/71 (40.85%), Postives = 38/71 (53.52%), Query Frame = 1

Query:  262 DCHCSNCLLVIERQRVMAAQVALRRQQTTSACKLPTNTKTEKSSYKRLIRQSISTVKSKKSHTIFRKQSEK 474
            DC C+ C L+ ERQRVMAAQVALRRQQ           ++E     RL+ Q  S   ++ S    R +S +
Sbjct:  109 DCACAKCTLIAERQRVMAAQVALRRQQ--------AQEESEARGLHRLLYQGSSGSGAQASGGSGRTESPQ 171

[Close]

BLAST of Dmd-3 vs. UniProt/SwissProt
Match: sp|Q5VZB9|DMRTA_HUMAN (Doublesex- and mab-3-related transcription factor A1 OS=Homo sapiens OX=9606 GN=DMRTA1 PE=2 SV=1)

HSP 1 Score: 60.077 bits (144), Expect = 4.486e-18
Identity = 25/27 (92.59%), Postives = 25/27 (92.59%), Query Frame = 3

Query:  183 RTPKCARCRNHGVVSCLKGHKRFCHWR 263
            RTPKCARCRNHGVVS LKGHKRFC WR
Sbjct:   93 RTPKCARCRNHGVVSALKGHKRFCRWR 119

HSP 2 Score: 52.7582 bits (125), Expect = 4.486e-18
Identity = 21/28 (75.00%), Postives = 23/28 (82.14%), Query Frame = 1

Query:  262 DCHCSNCLLVIERQRVMAAQVALRRQQT 345
            DC C+ C L+ ERQRVMAAQVALRRQQ 
Sbjct:  120 DCACAKCTLIAERQRVMAAQVALRRQQA 147

[Close]

BLAST of Dmd-3 vs. UniProt/SwissProt
Match: sp|A8TSS9|DMTA2_MONAL (Doublesex- and mab-3-related transcription factor A2 OS=Monopterus albus OX=43700 GN=dmrta2 PE=2 SV=1)

HSP 1 Score: 58.9214 bits (141), Expect = 5.567e-18
Identity = 23/27 (85.19%), Postives = 25/27 (92.59%), Query Frame = 3

Query:  183 RTPKCARCRNHGVVSCLKGHKRFCHWR 263
            RTPKCARCRNHGVVS LKGHKR+C W+
Sbjct:   53 RTPKCARCRNHGVVSALKGHKRYCRWK 79

HSP 2 Score: 53.5286 bits (127), Expect = 5.567e-18
Identity = 21/28 (75.00%), Postives = 23/28 (82.14%), Query Frame = 1

Query:  262 DCHCSNCLLVIERQRVMAAQVALRRQQT 345
            DC C+ C L+ ERQRVMAAQVALRRQQ 
Sbjct:   80 DCMCAKCTLIAERQRVMAAQVALRRQQA 107

[Close]

BLAST of Dmd-3 vs. TrEMBL
Match: R4PLE1 (Dmd-3 (Fragment) OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 413.305 bits (1061), Expect = 3.841e-143
Identity = 220/221 (99.55%), Postives = 220/221 (99.55%), Query Frame = 1

Query:  262 DCHCSNCLLVIERQRVMAAQVALRRQQTTSACKLPTNTKTEKSSYKRLIRQSISTVKSKKSHTIFRKQSEKKESNMKNNSSGSVESILERLPVDLGTQFTERLRKRKAFADEXXXXXXXXXXXXXXXIYSTKVENSNYLIYNLNKNDNFKEVYHNMVSEQLKFWMEQLKKNLGVFGSGKSEEISLYQNYFSRLYPEITNYNSKNQNKVINSEKSFFIERLL 924
            DCHCSNCLLVIERQRVMAAQVALRRQQTTSACKLPTNTKTEKSSYKRLIRQSISTVKSKKSHTIFRKQSEKKESNMKNNSSGSVESILERLPVDLGTQFTERLRKRKAFADENLDSISSLNNQL SLIYSTKVENSNYLIYNLNKNDNFKEVYHNMVSEQLKFWMEQLKKNLGVFGSGKSEEISLYQNYFSRLYPEITNYNSKNQNKVINSEKSFFIERLL
Sbjct:   35 DCHCSNCLLVIERQRVMAAQVALRRQQTTSACKLPTNTKTEKSSYKRLIRQSISTVKSKKSHTIFRKQSEKKESNMKNNSSGSVESILERLPVDLGTQFTERLRKRKAFADENLDSISSLNNQLTSLIYSTKVENSNYLIYNLNKNDNFKEVYHNMVSEQLKFWMEQLKKNLGVFGSGKSEEISLYQNYFSRLYPEITNYNSKNQNKVINSEKSFFIERLL 255

HSP 2 Score: 79.7221 bits (195), Expect = 1.640e-13
Identity = 34/34 (100.00%), Postives = 34/34 (100.00%), Query Frame = 3

Query:  162 MKLYNVSRTPKCARCRNHGVVSCLKGHKRFCHWR 263
            MKLYNVSRTPKCARCRNHGVVSCLKGHKRFCHWR
Sbjct:    1 MKLYNVSRTPKCARCRNHGVVSCLKGHKRFCHWR 34

[Close]

BLAST of Dmd-3 vs. TrEMBL
Match: A0A3Q2E9U6 (Doublesex and mab-3 related transcription factor 2a OS=Cyprinodon variegatus OX=28743 PE=4 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 1.066e-23
Identity = 50/117 (42.74%), Postives = 65/117 (55.56%), Query Frame = 1

Query:  262 DCHCSNCLLVIERQRVMAAQVALRRQQTT------SACKLPTNTKTEKSSYKRLIRQSISTVKSKKSHTIFRKQSEKKESNMKNNSSGSVESILERLPVDLGTQFTERLRKRKAFAD 594
            DC C+NCLLV+ERQRVMAAQVALRRQQ T      S  ++P     E+  Y+R IR S    KS     +   +  + ES +  N+S         LP  L    ++R+RKR+AFAD
Sbjct:   96 DCQCANCLLVVERQRVMAAQVALRRQQATEDKKGISGKQIP----AERRIYQRHIRPSTMLAKS----ILEGYRPVQPESFLAANAS---------LPPPL----SDRMRKRRAFAD 191

HSP 2 Score: 65.0846 bits (157), Expect = 1.066e-23
Identity = 27/29 (93.10%), Postives = 28/29 (96.55%), Query Frame = 3

Query:  177 VSRTPKCARCRNHGVVSCLKGHKRFCHWR 263
            +SRTPKCARCRNHGVVSCLKGHKRFC WR
Sbjct:   67 LSRTPKCARCRNHGVVSCLKGHKRFCRWR 95

[Close]

BLAST of Dmd-3 vs. TrEMBL
Match: A0A3Q2E9V0 (Doublesex and mab-3 related transcription factor 2a OS=Cyprinodon variegatus OX=28743 PE=4 SV=1)

HSP 1 Score: 75.0998 bits (183), Expect = 1.123e-23
Identity = 50/117 (42.74%), Postives = 65/117 (55.56%), Query Frame = 1

Query:  262 DCHCSNCLLVIERQRVMAAQVALRRQQTT------SACKLPTNTKTEKSSYKRLIRQSISTVKSKKSHTIFRKQSEKKESNMKNNSSGSVESILERLPVDLGTQFTERLRKRKAFAD 594
            DC C+NCLLV+ERQRVMAAQVALRRQQ T      S  ++P     E+  Y+R IR S    KS     +   +  + ES +  N+S         LP  L    ++R+RKR+AFAD
Sbjct:   37 DCQCANCLLVVERQRVMAAQVALRRQQATEDKKGISGKQIP----AERRIYQRHIRPSTMLAKS----ILEGYRPVQPESFLAANAS---------LPPPL----SDRMRKRRAFAD 132

HSP 2 Score: 64.6994 bits (156), Expect = 1.123e-23
Identity = 27/29 (93.10%), Postives = 28/29 (96.55%), Query Frame = 3

Query:  177 VSRTPKCARCRNHGVVSCLKGHKRFCHWR 263
            +SRTPKCARCRNHGVVSCLKGHKRFC WR
Sbjct:    8 LSRTPKCARCRNHGVVSCLKGHKRFCRWR 36

[Close]

BLAST of Dmd-3 vs. TrEMBL
Match: Q6YHU7 (DM-related transcriptional factor Dmrt2 OS=Oreochromis niloticus OX=8128 PE=2 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 1.270e-23
Identity = 47/117 (40.17%), Postives = 61/117 (52.14%), Query Frame = 1

Query:  262 DCHCSNCLLVIERQRVMAAQVALRRQQTT------SACKLPTNTKTEKSSYKRLIRQSISTVKSKKSHTIFRKQSEKKESNMKNNSSGSVESILERLPVDLGTQFTERLRKRKAFAD 594
            DC C+NCLLV+ERQRVMAAQVALRRQQ T      S   +P   +T    Y+R IR S    KS     + R +  + +  +  N S             L    ++R+RKR+AFAD
Sbjct:   38 DCQCANCLLVVERQRVMAAQVALRRQQATEDKKGISGKPIPAERRT---IYQRHIRPSTMLAKS----ILERYRPVQSDPFLAANPS-------------LPPPLSDRMRKRRAFAD 134

HSP 2 Score: 65.0846 bits (157), Expect = 1.270e-23
Identity = 27/29 (93.10%), Postives = 28/29 (96.55%), Query Frame = 3

Query:  177 VSRTPKCARCRNHGVVSCLKGHKRFCHWR 263
            +SRTPKCARCRNHGVVSCLKGHKRFC WR
Sbjct:    9 LSRTPKCARCRNHGVVSCLKGHKRFCRWR 37

[Close]

BLAST of Dmd-3 vs. TrEMBL
Match: A0A2G9RHP0 (DM domain-containing protein (Fragment) OS=Lithobates catesbeianus OX=8400 GN=AB205_0141730 PE=4 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 1.518e-23
Identity = 49/115 (42.61%), Postives = 61/115 (53.04%), Query Frame = 1

Query:  262 DCHCSNCLLVIERQRVMAAQVALRRQQTTSACKL----PTNTKTEKSSYKRLIRQSISTVKSKKSHTIFRKQSEKKESNMKNNSSGSVESILERLPVDLGTQFTERLRKRKAFAD 594
            DC C+NCLLV+ERQRVMAAQVALRRQQ T   K     PT T+  K+ Y+R IR      KS     +   +    +S M  NS              L    ++R+RKR+AFAD
Sbjct:  152 DCQCANCLLVVERQRVMAAQVALRRQQATEDKKGLTGKPTGTE-RKTVYQRHIRTPSLLAKS----ILEGYRPLPADSYMGPNSP-------------LPAPVSDRMRKRRAFAD 248

HSP 2 Score: 65.0846 bits (157), Expect = 1.518e-23
Identity = 27/29 (93.10%), Postives = 28/29 (96.55%), Query Frame = 3

Query:  177 VSRTPKCARCRNHGVVSCLKGHKRFCHWR 263
            +SRTPKCARCRNHGVVSCLKGHKRFC WR
Sbjct:  123 LSRTPKCARCRNHGVVSCLKGHKRFCRWR 151

[Close]

BLAST of Dmd-3 vs. Ensembl Cavefish
Match: dmrt2a (doublesex- and mab-3-related transcription factor 2 [Source:NCBI gene;Acc:103040902])

HSP 1 Score: 70.8626 bits (172), Expect = 4.507e-26
Identity = 45/114 (39.47%), Postives = 58/114 (50.88%), Query Frame = 1

Query:  262 DCHCSNCLLVIERQRVMAAQVALRRQQTTSACKLPTNTKTE---KSSYKRLIRQSISTVKSKKSHTIFRKQSEKKESNMKNNSSGSVESILERLPVDLGTQFTERLRKRKAFAD 594
            DC C+NCLLV+ERQRVMAAQVALRRQQ T   K  +   T    ++ Y+R IR S    KS                 ++       +  L   P  L    ++R+RKR+AFAD
Sbjct:   88 DCQCANCLLVVERQRVMAAQVALRRQQATEDKKGISGKPTPAERRTIYQRHIRPSTMLAKSI----------------LEGYRPVQADPFLGTSPA-LPPPLSDRMRKRRAFAD 184

HSP 2 Score: 66.2402 bits (160), Expect = 4.507e-26
Identity = 31/54 (57.41%), Postives = 36/54 (66.67%), Query Frame = 3

Query:  102 GEFISNFKPEFSQRYRNYFAMKLYNVSRTPKCARCRNHGVVSCLKGHKRFCHWR 263
            GE     K E    +  + + +   +SRTPKCARCRNHGVVSCLKGHKRFC WR
Sbjct:   34 GEEDPGAKDEDRAGHTAHMSGEQKKLSRTPKCARCRNHGVVSCLKGHKRFCRWR 87

[Close]

BLAST of Dmd-3 vs. Ensembl Cavefish
Match: dmrt2a (doublesex- and mab-3-related transcription factor 2 [Source:NCBI gene;Acc:103040902])

HSP 1 Score: 70.4774 bits (171), Expect = 5.875e-26
Identity = 45/114 (39.47%), Postives = 58/114 (50.88%), Query Frame = 1

Query:  262 DCHCSNCLLVIERQRVMAAQVALRRQQTTSACKLPTNTKTE---KSSYKRLIRQSISTVKSKKSHTIFRKQSEKKESNMKNNSSGSVESILERLPVDLGTQFTERLRKRKAFAD 594
            DC C+NCLLV+ERQRVMAAQVALRRQQ T   K  +   T    ++ Y+R IR S    KS                 ++       +  L   P  L    ++R+RKR+AFAD
Sbjct:   38 DCQCANCLLVVERQRVMAAQVALRRQQATEDKKGISGKPTPAERRTIYQRHIRPSTMLAKSI----------------LEGYRPVQADPFLGTSPA-LPPPLSDRMRKRRAFAD 134

HSP 2 Score: 66.2402 bits (160), Expect = 5.875e-26
Identity = 31/38 (81.58%), Postives = 31/38 (81.58%), Query Frame = 3

Query:  150 NYFAMKLYNVSRTPKCARCRNHGVVSCLKGHKRFCHWR 263
            N F  KL   SRTPKCARCRNHGVVSCLKGHKRFC WR
Sbjct:    3 NTFQKKL---SRTPKCARCRNHGVVSCLKGHKRFCRWR 37

[Close]

BLAST of Dmd-3 vs. Ensembl Cavefish
Match: dmrt2b (doublesex and mab-3 related transcription factor 2b [Source:ZFIN;Acc:ZDB-GENE-080813-1])

HSP 1 Score: 63.5438 bits (153), Expect = 6.876e-21
Identity = 43/114 (37.72%), Postives = 56/114 (49.12%), Query Frame = 1

Query:  262 DCHCSNCLLVIERQRVMAAQVALRRQQTTSACKLPTNTKTEKSS--YKRLIRQSISTVKSKKSHTIFRKQSEKKESNMKNNSSGSVESILERLPVDLG-TQFTERLRKRKAFAD 594
            DC C+NC LV+ERQRVMAAQVALRRQQ           K+++S+   +R   Q  S   S  + +I                 G    +LE  P  L     + R+RKR+AFAD
Sbjct:  102 DCRCANCALVVERQRVMAAQVALRRQQAAE------GKKSQRSASFLRRSAYQCYSRAPSLLAKSIL---------------EGYTPPVLEDWPKRLHYPPLSVRMRKRRAFAD 194

HSP 2 Score: 56.225 bits (134), Expect = 6.876e-21
Identity = 24/28 (85.71%), Postives = 25/28 (89.29%), Query Frame = 3

Query:  180 SRTPKCARCRNHGVVSCLKGHKRFCHWR 263
            SR+PKCARCRNHGVVS LKGHKR C WR
Sbjct:   74 SRSPKCARCRNHGVVSRLKGHKRLCRWR 101

[Close]

BLAST of Dmd-3 vs. Ensembl Cavefish
Match: dmrta2 (DMRT like family A2 [Source:NCBI gene;Acc:103023395])

HSP 1 Score: 58.5362 bits (140), Expect = 1.103e-18
Identity = 23/27 (85.19%), Postives = 25/27 (92.59%), Query Frame = 3

Query:  183 RTPKCARCRNHGVVSCLKGHKRFCHWR 263
            RTPKCARCRNHGVVS LKGHKR+C W+
Sbjct:   59 RTPKCARCRNHGVVSALKGHKRYCRWK 85

HSP 2 Score: 53.5286 bits (127), Expect = 1.103e-18
Identity = 21/28 (75.00%), Postives = 23/28 (82.14%), Query Frame = 1

Query:  262 DCHCSNCLLVIERQRVMAAQVALRRQQT 345
            DC C+ C L+ ERQRVMAAQVALRRQQ 
Sbjct:   86 DCMCAKCTLIAERQRVMAAQVALRRQQA 113

[Close]

BLAST of Dmd-3 vs. Ensembl Cavefish
Match: dmrt3a (doublesex and mab-3 related transcription factor 3 [Source:NCBI gene;Acc:103040280])

HSP 1 Score: 56.6102 bits (135), Expect = 1.678e-17
Identity = 22/29 (75.86%), Postives = 25/29 (86.21%), Query Frame = 1

Query:  262 DCHCSNCLLVIERQRVMAAQVALRRQQTT 348
            DC C  C+L+IERQRVMAAQVALRRQQ +
Sbjct:   51 DCTCDKCILIIERQRVMAAQVALRRQQAS 79

HSP 2 Score: 51.6026 bits (122), Expect = 1.678e-17
Identity = 21/29 (72.41%), Postives = 26/29 (89.66%), Query Frame = 3

Query:  177 VSRTPKCARCRNHGVVSCLKGHKRFCHWR 263
            + RTPKCARCRNHGV+S LKGHKR+C ++
Sbjct:   22 LQRTPKCARCRNHGVLSWLKGHKRYCRFK 50

[Close]

BLAST of Dmd-3 vs. Ensembl Sea Lamprey
Match: dmrt2b (doublesex and mab-3 related transcription factor 2b [Source:ZFIN;Acc:ZDB-GENE-080813-1])

HSP 1 Score: 68.1662 bits (165), Expect = 2.384e-23
Identity = 45/115 (39.13%), Postives = 60/115 (52.17%), Query Frame = 1

Query:  262 DCHCSNCLLVIERQRVMAAQVALRRQQTTSACKLPTNTKTEKSSYKRLIRQSISTVKSKKSHTI---FRKQSEKKESNMKNNSSGSVESILERLPVDLGTQFTERLRKRKAFADE 597
            DC C+NCLLV+ERQRVMAAQVALRRQQ T A   P     E ++ +R +  S +   S  + +I   +R  S         +           LP       +ER+RKR+AFAD+
Sbjct:   76 DCQCANCLLVVERQRVMAAQVALRRQQATEANSGPQG--KEATANRRPVYVSQARTPSLLAKSILDGYRPTSASAGGGGGGDDHFGSGPAPALLP-----PLSERMRKRRAFADK 183

HSP 2 Score: 57.3806 bits (137), Expect = 2.384e-23
Identity = 25/29 (86.21%), Postives = 27/29 (93.10%), Query Frame = 3

Query:  177 VSRTPKCARCRNHGVVSCLKGHKRFCHWR 263
            ++RTPKCARCRNHGV SCLKGHKRFC WR
Sbjct:   48 MARTPKCARCRNHGV-SCLKGHKRFCRWR 75

[Close]

BLAST of Dmd-3 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000004009.1 (pep scaffold:Pmarinus_7.0:GL483432:15080:15595:1 gene:ENSPMAG00000003668.1 transcript:ENSPMAT00000004009.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 57.7658 bits (138), Expect = 5.219e-18
Identity = 24/28 (85.71%), Postives = 25/28 (89.29%), Query Frame = 3

Query:  180 SRTPKCARCRNHGVVSCLKGHKRFCHWR 263
             RTPKCARCRNHGVVS LKGHKR+C WR
Sbjct:   49 QRTPKCARCRNHGVVSALKGHKRYCRWR 76

HSP 2 Score: 49.6766 bits (117), Expect = 5.219e-18
Identity = 21/28 (75.00%), Postives = 23/28 (82.14%), Query Frame = 1

Query:  262 DCHCSNCLLVIERQRVMAAQVALRRQQT 345
            DC C+ C L+ ERQRVMAAQVALRRQQ 
Sbjct:   77 DCLCAKCTLIAERQRVMAAQVALRRQQA 104

[Close]

BLAST of Dmd-3 vs. Ensembl Nematostella
Match: EDO27141 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SV98])

HSP 1 Score: 53.5286 bits (127), Expect = 1.043e-15
Identity = 22/27 (81.48%), Postives = 25/27 (92.59%), Query Frame = 3

Query:  183 RTPKCARCRNHGVVSCLKGHKRFCHWR 263
            R+PKCARCRNHGV+S LKGHKRFC W+
Sbjct:    1 RSPKCARCRNHGVISILKGHKRFCKWK 27

HSP 2 Score: 47.3654 bits (111), Expect = 1.043e-15
Identity = 21/29 (72.41%), Postives = 24/29 (82.76%), Query Frame = 1

Query:  262 DCHCSNCLLVIERQRVMAAQVALRRQQTT 348
            DC C +C L+ ERQRVMAAQVALRRQQ +
Sbjct:   28 DCTCPDCNLIAERQRVMAAQVALRRQQES 56

[Close]

BLAST of Dmd-3 vs. Ensembl Nematostella
Match: EDO32384 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SV98])

HSP 1 Score: 53.5286 bits (127), Expect = 1.043e-15
Identity = 22/27 (81.48%), Postives = 25/27 (92.59%), Query Frame = 3

Query:  183 RTPKCARCRNHGVVSCLKGHKRFCHWR 263
            R+PKCARCRNHGV+S LKGHKRFC W+
Sbjct:    1 RSPKCARCRNHGVISILKGHKRFCKWK 27

HSP 2 Score: 47.3654 bits (111), Expect = 1.043e-15
Identity = 21/29 (72.41%), Postives = 24/29 (82.76%), Query Frame = 1

Query:  262 DCHCSNCLLVIERQRVMAAQVALRRQQTT 348
            DC C +C L+ ERQRVMAAQVALRRQQ +
Sbjct:   28 DCTCPDCNLIAERQRVMAAQVALRRQQES 56

[Close]

BLAST of Dmd-3 vs. Ensembl Nematostella
Match: EDO41281 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S4Z8])

HSP 1 Score: 49.6766 bits (117), Expect = 7.193e-14
Identity = 22/25 (88.00%), Postives = 23/25 (92.00%), Query Frame = 3

Query:  189 PKCARCRNHGVVSCLKGHKRFCHWR 263
            PKCARCRNHGVVS LKGHKR+C WR
Sbjct:    2 PKCARCRNHGVVSWLKGHKRYCRWR 26

HSP 2 Score: 44.669 bits (104), Expect = 7.193e-14
Identity = 18/24 (75.00%), Postives = 21/24 (87.50%), Query Frame = 1

Query:  262 DCHCSNCLLVIERQRVMAAQVALR 333
            DC+C+ C L+ ERQRVMAAQVALR
Sbjct:   27 DCNCAQCTLIAERQRVMAAQVALR 50

[Close]

BLAST of Dmd-3 vs. Ensembl Nematostella
Match: EDO30270 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T198])

HSP 1 Score: 48.9062 bits (115), Expect = 4.141e-13
Identity = 21/30 (70.00%), Postives = 27/30 (90.00%), Query Frame = 3

Query:  174 NVSRTPKCARCRNHGVVSCLKGHKRFCHWR 263
            N+ R+P+CARCRNHG+VS LKGHKR+C W+
Sbjct:   10 NLIRSPRCARCRNHGIVSYLKGHKRYCQWK 39

HSP 2 Score: 42.743 bits (99), Expect = 4.141e-13
Identity = 18/27 (66.67%), Postives = 23/27 (85.19%), Query Frame = 1

Query:  262 DCHCSNCLLVIERQRVMAAQVALRRQQ 342
            DC C+ C L+IERQR+MAAQVAL+R +
Sbjct:   40 DCTCTKCSLIIERQRLMAAQVALKRDE 66

[Close]

BLAST of Dmd-3 vs. Ensembl Nematostella
Match: EDO37386 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SFT8])

HSP 1 Score: 46.595 bits (109), Expect = 5.982e-13
Identity = 20/27 (74.07%), Postives = 22/27 (81.48%), Query Frame = 3

Query:  183 RTPKCARCRNHGVVSCLKGHKRFCHWR 263
            R PKCARCRNHG +S LKGHK +C WR
Sbjct:    1 RIPKCARCRNHGALSWLKGHKHYCRWR 27

HSP 2 Score: 45.0542 bits (105), Expect = 5.982e-13
Identity = 19/27 (70.37%), Postives = 22/27 (81.48%), Query Frame = 1

Query:  262 DCHCSNCLLVIERQRVMAAQVALRRQQ 342
            DC CS CLL+ ERQR+ AA+VAL RQQ
Sbjct:   28 DCTCSKCLLIAERQRITAARVALLRQQ 54

[Close]

BLAST of Dmd-3 vs. Ensembl Medaka
Match: dmrt2a (doublesex- and mab-3-related transcription factor 2 [Source:NCBI gene;Acc:101163154])

HSP 1 Score: 69.707 bits (169), Expect = 1.672e-25
Identity = 47/117 (40.17%), Postives = 63/117 (53.85%), Query Frame = 1

Query:  262 DCHCSNCLLVIERQRVMAAQVALRRQQTT------SACKLPTNTKTEKSSYKRLIRQSISTVKSKKSHTIFRKQSEKKESNMKNNSSGSVESILERLPVDLGTQFTERLRKRKAFAD 594
            DC C+NCLLV+ERQRVMAAQVALRRQQ T      S  ++P     E+  Y+R +R S    KS     I   +  + +  +  + S         LP  L    ++R+RKR+AFAD
Sbjct:  106 DCQCANCLLVVERQRVMAAQVALRRQQATEDKKGISGKEIPA----ERRIYQRHVRPSSMLAKS-----IL--EGYRPDPFLAASPS---------LPPPL----SDRMRKRRAFAD 198

HSP 2 Score: 65.4698 bits (158), Expect = 1.672e-25
Identity = 27/29 (93.10%), Postives = 28/29 (96.55%), Query Frame = 3

Query:  177 VSRTPKCARCRNHGVVSCLKGHKRFCHWR 263
            +SRTPKCARCRNHGVVSCLKGHKRFC WR
Sbjct:   77 LSRTPKCARCRNHGVVSCLKGHKRFCRWR 105

[Close]

BLAST of Dmd-3 vs. Ensembl Medaka
Match: dmrt2b (doublesex- and mab-3-related transcription factor 2 [Source:NCBI gene;Acc:101156565])

HSP 1 Score: 66.2402 bits (160), Expect = 1.535e-23
Identity = 42/119 (35.29%), Postives = 62/119 (52.10%), Query Frame = 1

Query:  262 DCHCSNCLLVIERQRVMAAQVALRRQQTTSACKLPTNTK--------TEKSSYKRLIRQSISTVKSKKSHTIFRKQSEKKESNMKNNSSGSVESILERLPVDLGTQFTERLRKRKAFAD 594
            DC C+ CLLV+ERQRVMAAQVALRRQQ     ++P  +K          +++Y    R +  ++ +KK+    +     + S     SS S +   +  P       + R+RKR+AFAD
Sbjct:   88 DCRCACCLLVVERQRVMAAQVALRRQQAAEVRRVPGQSKRGVKCAAPLRRAAYHCYSRAAEPSIMTKKTLQGLKPAMPPEVS----TSSWSKQPQRDHFPC---ASISARMRKRRAFAD 199

HSP 2 Score: 62.3882 bits (150), Expect = 1.535e-23
Identity = 26/33 (78.79%), Postives = 29/33 (87.88%), Query Frame = 3

Query:  165 KLYNVSRTPKCARCRNHGVVSCLKGHKRFCHWR 263
            K   ++R+PKCARCRNHGVVSCLKGHKRFC WR
Sbjct:   55 KPRKMTRSPKCARCRNHGVVSCLKGHKRFCRWR 87

[Close]

BLAST of Dmd-3 vs. Ensembl Medaka
Match: dmrt2b (doublesex- and mab-3-related transcription factor 2 [Source:NCBI gene;Acc:101156565])

HSP 1 Score: 66.2402 bits (160), Expect = 1.547e-23
Identity = 42/120 (35.00%), Postives = 63/120 (52.50%), Query Frame = 1

Query:  262 DCHCSNCLLVIERQRVMAAQVALRRQQTTSACKLPTNTK--------TEKSSYKRLIRQSISTVKSKKSHTIFRKQSEKKESNMKNNSSGSVESILERLPVDLGTQFTERLRKRKAFADE 597
            DC C+ CLLV+ERQRVMAAQVALRRQQ     ++P  +K          +++Y    R +  ++ +KK+    +     + S     SS S +   +  P       + R+RKR+AFAD+
Sbjct:   97 DCRCACCLLVVERQRVMAAQVALRRQQAAEVRRVPGQSKRGVKCAAPLRRAAYHCYSRAAEPSIMTKKTLQGLKPAMPPEVS----TSSWSKQPQRDHFPC---ASISARMRKRRAFADK 209

HSP 2 Score: 62.3882 bits (150), Expect = 1.547e-23
Identity = 26/33 (78.79%), Postives = 29/33 (87.88%), Query Frame = 3

Query:  165 KLYNVSRTPKCARCRNHGVVSCLKGHKRFCHWR 263
            K   ++R+PKCARCRNHGVVSCLKGHKRFC WR
Sbjct:   64 KPRKMTRSPKCARCRNHGVVSCLKGHKRFCRWR 96

[Close]

BLAST of Dmd-3 vs. Ensembl Medaka
Match: DMRTA1 (DMRT like family A1 [Source:NCBI gene;Acc:101165100])

HSP 1 Score: 60.4622 bits (145), Expect = 5.701e-19
Identity = 25/27 (92.59%), Postives = 25/27 (92.59%), Query Frame = 3

Query:  183 RTPKCARCRNHGVVSCLKGHKRFCHWR 263
            RTPKCARCRNHGVVS LKGHKRFC WR
Sbjct:   48 RTPKCARCRNHGVVSALKGHKRFCRWR 74

HSP 2 Score: 52.373 bits (124), Expect = 5.701e-19
Identity = 21/28 (75.00%), Postives = 23/28 (82.14%), Query Frame = 1

Query:  262 DCHCSNCLLVIERQRVMAAQVALRRQQT 345
            DC C+ C L+ ERQRVMAAQVALRRQQ 
Sbjct:   75 DCVCAKCTLIAERQRVMAAQVALRRQQA 102

[Close]

BLAST of Dmd-3 vs. Ensembl Medaka
Match: dmrta2 (DMRT like family A2 [Source:NCBI gene;Acc:111947399])

HSP 1 Score: 58.9214 bits (141), Expect = 1.027e-18
Identity = 23/27 (85.19%), Postives = 25/27 (92.59%), Query Frame = 3

Query:  183 RTPKCARCRNHGVVSCLKGHKRFCHWR 263
            RTPKCARCRNHGVVS LKGHKR+C W+
Sbjct:   53 RTPKCARCRNHGVVSALKGHKRYCRWK 79

HSP 2 Score: 53.5286 bits (127), Expect = 1.027e-18
Identity = 21/28 (75.00%), Postives = 23/28 (82.14%), Query Frame = 1

Query:  262 DCHCSNCLLVIERQRVMAAQVALRRQQT 345
            DC C+ C L+ ERQRVMAAQVALRRQQ 
Sbjct:   80 DCMCAKCTLIAERQRVMAAQVALRRQQA 107

[Close]

BLAST of Dmd-3 vs. Planmine SMEST
Match: SMESG000023052.1 (SMESG000023052.1)

HSP 1 Score: 379.407 bits (973), Expect = 5.417e-134
Identity = 205/205 (100.00%), Postives = 205/205 (100.00%), Query Frame = 1

Query:  310 MAAQVALRRQQTTSACKLPTNTKTEKSSYKRLIRQSISTVKSKKSHTIFRKQSEKKESNMKNNSSGSVESILERLPVDLGTQFTERLRKRKAFADEXXXXXXXXXXXXXXXIYSTKVENSNYLIYNLNKNDNFKEVYHNMVSEQLKFWMEQLKKNLGVFGSGKSEEISLYQNYFSRLYPEITNYNSKNQNKVINSEKSFFIERLL 924
            MAAQVALRRQQTTSACKLPTNTKTEKSSYKRLIRQSISTVKSKKSHTIFRKQSEKKESNMKNNSSGSVESILERLPVDLGTQFTERLRKRKAFADENLDSISSLNNQLNSLIYSTKVENSNYLIYNLNKNDNFKEVYHNMVSEQLKFWMEQLKKNLGVFGSGKSEEISLYQNYFSRLYPEITNYNSKNQNKVINSEKSFFIERLL
Sbjct:    1 MAAQVALRRQQTTSACKLPTNTKTEKSSYKRLIRQSISTVKSKKSHTIFRKQSEKKESNMKNNSSGSVESILERLPVDLGTQFTERLRKRKAFADENLDSISSLNNQLNSLIYSTKVENSNYLIYNLNKNDNFKEVYHNMVSEQLKFWMEQLKKNLGVFGSGKSEEISLYQNYFSRLYPEITNYNSKNQNKVINSEKSFFIERLL 205

[Close]

BLAST of Dmd-3 vs. Planmine SMEST
Match: SMESG000006521.1 (SMESG000006521.1)

HSP 1 Score: 59.3066 bits (142), Expect = 4.224e-18
Identity = 28/55 (50.91%), Postives = 37/55 (67.27%), Query Frame = 3

Query:  111 ISNFKPEFSQRYRNYFAMKLYNVS----RTPKCARCRNHGVVSCLKGHKRFCHWR 263
            +S+  PEFS+ +       LY ++    R PKCARCRNHG+VS LKGHKR CH++
Sbjct:   12 LSDQTPEFSRNFLKLMQPPLYPITEKGARKPKCARCRNHGMVSWLKGHKRHCHYK 66

HSP 2 Score: 50.447 bits (119), Expect = 4.224e-18
Identity = 20/29 (68.97%), Postives = 23/29 (79.31%), Query Frame = 1

Query:  262 DCHCSNCLLVIERQRVMAAQVALRRQQTT 348
            DC C+ C L+ ERQRVMAAQVAL+RQQ  
Sbjct:   67 DCRCAKCNLIAERQRVMAAQVALKRQQAA 95

[Close]

BLAST of Dmd-3 vs. Planmine SMEST
Match: SMESG000075920.1 (SMESG000075920.1)

HSP 1 Score: 54.6842 bits (130), Expect = 8.814e-17
Identity = 22/27 (81.48%), Postives = 24/27 (88.89%), Query Frame = 3

Query:  183 RTPKCARCRNHGVVSCLKGHKRFCHWR 263
            RTPKCARCRNHG VS LKGHKR+C W+
Sbjct:   50 RTPKCARCRNHGYVSPLKGHKRYCRWK 76

HSP 2 Score: 50.447 bits (119), Expect = 8.814e-17
Identity = 21/28 (75.00%), Postives = 23/28 (82.14%), Query Frame = 1

Query:  262 DCHCSNCLLVIERQRVMAAQVALRRQQT 345
            DC C+ C L+ ERQRVMAAQVALRRQQ 
Sbjct:   77 DCVCAKCALIAERQRVMAAQVALRRQQA 104

The following BLAST results are available for this feature:

BLAST of Dmd-3 vs. Ensembl Human
BLAST of Dmd-3 vs. Ensembl Celegans
BLAST of Dmd-3 vs. Ensembl Fly
BLAST of Dmd-3 vs. Ensembl Zebrafish
BLAST of Dmd-3 vs. Ensembl Xenopus
BLAST of Dmd-3 vs. Ensembl Mouse
BLAST of Dmd-3 vs. UniProt/SwissProt
BLAST of Dmd-3 vs. TrEMBL
BLAST of Dmd-3 vs. Ensembl Cavefish
BLAST of Dmd-3 vs. Ensembl Sea Lamprey
BLAST of Dmd-3 vs. Ensembl Yeast
BLAST of Dmd-3 vs. Ensembl Nematostella
BLAST of Dmd-3 vs. Ensembl Medaka
BLAST of Dmd-3 vs. Planmine SMEST

BLAST of Dmd-3 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
DMRT2	2.694e-23	93.10	doublesex and mab-3 related transcription factor 2...	[more]
DMRT2	2.694e-23	93.10	doublesex and mab-3 related transcription factor 2...	[more]
DMRT2	4.648e-22	93.10	doublesex and mab-3 related transcription factor 2...	[more]
DMRT2	4.648e-22	93.10	doublesex and mab-3 related transcription factor 2...	[more]
DMRT2	4.648e-22	93.10	doublesex and mab-3 related transcription factor 2...	[more]

BLAST of Dmd-3 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5


Match Name	E-value	Identity	Description
dmd-5	1.091e-17	77.78	DM (Doublesex/MAB-3) Domain family [Source:UniPro...	[more]
dmd-4	4.566e-13	71.43	DM (Doublesex/MAB-3) Domain family [Source:UniPro...	[more]
dmd-3	2.691e-8	55.56	DM (Doublesex/MAB-3) Domain family [Source:UniPro...	[more]
dmd-3	5.417e-8	55.56	DM (Doublesex/MAB-3) Domain family [Source:UniPro...	[more]
dmd-10	7.645e-7	48.15	DM (Doublesex/MAB-3) Domain family [Source:UniPro...	[more]

BLAST of Dmd-3 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5


Match Name	E-value	Identity	Description
dmrt11E	1.447e-18	85.19	gene:FBgn0030477 transcript:FBtr0073768	[more]
dmrt99B	1.573e-18	88.89	gene:FBgn0039683 transcript:FBtr0085454	[more]
dmrt93B	5.969e-14	62.07	gene:FBgn0038851 transcript:FBtr0084007	[more]
dsx	1.314e-9	68.00	gene:FBgn0000504 transcript:FBtr0330073	[more]
dsx	1.314e-9	68.00	gene:FBgn0000504 transcript:FBtr0081759	[more]

BLAST of Dmd-3 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
dmrt2a	2.917e-25	40.35	doublesex and mab-3 related transcription factor 2...	[more]
dmrt2a	2.917e-25	40.35	doublesex and mab-3 related transcription factor 2...	[more]
dmrt2a	2.917e-25	40.35	doublesex and mab-3 related transcription factor 2...	[more]
dmrt2b	4.219e-22	40.35	doublesex and mab-3 related transcription factor 2...	[more]
dmrt2b	4.219e-22	40.35	doublesex and mab-3 related transcription factor 2...	[more]

BLAST of Dmd-3 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
kdm2b	2.485e-25	39.47	lysine (K)-specific demethylase 2B [Source:Xenbase...	[more]
dmrta1	3.351e-19	92.59	DMRT like family A1 [Source:NCBI gene;Acc:779901]	[more]
dynll1	2.021e-18	85.19	dynein light chain LC8-type 1 [Source:Xenbase;Acc:...	[more]
st3gal4	7.135e-17	78.57	ST3 beta-galactoside alpha-2,3-sialyltransferase 4...	[more]
DMRT1	2.845e-16	81.48	doublesex and mab-3 related transcription factor 1...	[more]

BLAST of Dmd-3 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Dmrt2	2.304e-24	38.60	doublesex and mab-3 related transcription factor 2...	[more]
Dmrta1	3.147e-19	92.59	doublesex and mab-3 related transcription factor l...	[more]
Dmrta2	3.926e-18	85.19	doublesex and mab-3 related transcription factor l...	[more]
Dmrt3	3.685e-17	78.57	doublesex and mab-3 related transcription factor 3...	[more]
Dmrt1	1.049e-16	81.48	doublesex and mab-3 related transcription factor 1...	[more]

BLAST of Dmd-3 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|Q8BG36\|DMRT2_MOUSE	1.355e-23	38.60	Doublesex- and mab-3-related transcription factor ...	[more]
sp\|Q9Y5R5\|DMRT2_HUMAN	1.150e-22	93.10	Doublesex- and mab-3-related transcription factor ...	[more]
sp\|Q8CFG4\|DMRTA_MOUSE	1.851e-18	92.59	Doublesex- and mab-3-related transcription factor ...	[more]
sp\|Q5VZB9\|DMRTA_HUMAN	4.486e-18	92.59	Doublesex- and mab-3-related transcription factor ...	[more]
sp\|A8TSS9\|DMTA2_MONAL	5.567e-18	85.19	Doublesex- and mab-3-related transcription factor ...	[more]

BLAST of Dmd-3 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
R4PLE1	3.841e-143	99.55	Dmd-3 (Fragment) OS=Schmidtea mediterranea OX=7932...	[more]
A0A3Q2E9U6	1.066e-23	42.74	Doublesex and mab-3 related transcription factor 2...	[more]
A0A3Q2E9V0	1.123e-23	42.74	Doublesex and mab-3 related transcription factor 2...	[more]
Q6YHU7	1.270e-23	40.17	DM-related transcriptional factor Dmrt2 OS=Oreochr...	[more]
A0A2G9RHP0	1.518e-23	42.61	DM domain-containing protein (Fragment) OS=Lithoba...	[more]

BLAST of Dmd-3 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
dmrt2a	4.507e-26	39.47	doublesex- and mab-3-related transcription factor ...	[more]
dmrt2a	5.875e-26	39.47	doublesex- and mab-3-related transcription factor ...	[more]
dmrt2b	6.876e-21	37.72	doublesex and mab-3 related transcription factor 2...	[more]
dmrta2	1.103e-18	85.19	DMRT like family A2 [Source:NCBI gene;Acc:10302339...	[more]
dmrt3a	1.678e-17	75.86	doublesex and mab-3 related transcription factor 3...	[more]

BLAST of Dmd-3 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 2


Match Name	E-value	Identity	Description
dmrt2b	2.384e-23	39.13	doublesex and mab-3 related transcription factor 2...	[more]
ENSPMAT00000004009.1	5.219e-18	85.71	pep scaffold:Pmarinus_7.0:GL483432:15080:15595:1 g...	[more]

BLAST of Dmd-3 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Dmd-3 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5


Match Name	E-value	Identity	Description
EDO27141	1.043e-15	81.48	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO32384	1.043e-15	81.48	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO41281	7.193e-14	88.00	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO30270	4.141e-13	70.00	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO37386	5.982e-13	74.07	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of Dmd-3 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5


Match Name	E-value	Identity	Description
dmrt2a	1.672e-25	40.17	doublesex- and mab-3-related transcription factor ...	[more]
dmrt2b	1.535e-23	35.29	doublesex- and mab-3-related transcription factor ...	[more]
dmrt2b	1.547e-23	35.00	doublesex- and mab-3-related transcription factor ...	[more]
DMRTA1	5.701e-19	92.59	DMRT like family A1 [Source:NCBI gene;Acc:10116510...	[more]
dmrta2	1.027e-18	85.19	DMRT like family A2 [Source:NCBI gene;Acc:11194739...	[more]

BLAST of Dmd-3 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3


Match Name	E-value	Identity	Description
SMESG000023052.1	5.417e-134	100.00	SMESG000023052.1	[more]
SMESG000006521.1	4.224e-18	50.91	SMESG000006521.1	[more]
SMESG000075920.1	8.814e-17	81.48	SMESG000075920.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Cross References

External references for this transcript


Database	Accession
SmedGD GBrowse	SMED30019413
GenBank	AGL61629.1

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30019413 ID=SMED30019413|Name=Dmd-3|organism=Schmidtea mediterranea sexual|type=transcript|length=959bp
TTGATTGGTTATTTGCACATTTTGGGTGCAAAAGATTGTGTGCAATTTCC
GGACCAATCCGAATTAAGCAGCTTATCCTTAATCAATAGAAGCCATTCAC
GGGAGAATTTATTTCAAATTTTAAACCCGAATTTTCACAAAGATATAGAA
ATTATTTTGCAATGAAATTATACAATGTCTCGAGAACACCAAAATGCGCC
AGATGTCGAAACCACGGTGTTGTATCTTGTTTAAAAGGGCATAAGCGTTT
TTGTCACTGGAGATTGTCACTGTAGTAACTGTTTACTGGTAATCGAAAGA
CAAAGAGTTATGGCTGCCCAAGTTGCTCTGAGGCGTCAGCAGACTACAAG
CGCTTGTAAACTGCCAACGAACACCAAGACGGAAAAGAGTTCGTATAAAC
GGCTTATCAGACAAAGCATATCGACAGTGAAATCCAAAAAATCCCATACC
ATATTTAGGAAGCAATCTGAAAAAAAAGAATCGAATATGAAGAACAATTC
ATCTGGGTCGGTCGAAAGCATTTTAGAACGGTTACCCGTTGATCTTGGAA
CTCAATTTACTGAACGTCTTCGGAAACGAAAAGCATTTGCTGATGAAAAT
CTTGATTCAATTTCCAGTTTAAACAATCAACTAAATTCGTTAATATATTC
AACGAAAGTTGAAAATTCCAATTATTTAATATATAATTTAAACAAAAATG
ACAATTTCAAAGAGGTGTATCACAATATGGTCAGTGAACAATTAAAGTTC
TGGATGGAACAGCTCAAAAAGAATTTAGGAGTATTTGGAAGTGGAAAATC
TGAAGAAATTTCCTTGTATCAAAATTATTTTAGTAGACTCTATCCAGAAA
TCACAAATTACAATTCAAAAAACCAAAATAAAGTTATAAACAGTGAAAAA
TCGTTCTTTATTGAGCGTCTTTTGAATTGAAACTTTAAAAATAACAAATT
CAAATATCC

protein sequence of SMED30019413-orf-1

>SMED30019413-orf-1 ID=SMED30019413-orf-1|Name=SMED30019413-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=207bp
MAAQVALRRQQTTSACKLPTNTKTEKSSYKRLIRQSISTVKSKKSHTIFR
KQSEKKESNMKNNSSGSVESILERLPVDLGTQFTERLRKRKAFADENLDS
ISSLNNQLNSLIYSTKVENSNYLIYNLNKNDNFKEVYHNMVSEQLKFWME
QLKKNLGVFGSGKSEEISLYQNYFSRLYPEITNYNSKNQNKVINSEKSFF
IERLLN*

Aliases

The feature 'Dmd-3' has the following synonyms


Synonym
Dmd-3

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000101	muscle cell
PLANA:0000420	parapharyngeal region

Vocabulary: **INTERPRO**
Term	Definition
IPR036407	DM_DNA-bd_sf
IPR026607	DMRT/dsx/mab-3
IPR001275	DM_DNA-bd

Vocabulary: **molecular function**
Term	Definition
GO:0043565	sequence-specific DNA binding
GO:0000977	RNA polymerase II regulatory region sequence-specific DNA binding
GO:0001228	transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
GO:0003677	DNA binding
GO:0003700	transcription factor activity, sequence-specific DNA binding
GO:0042803	protein homodimerization activity
GO:0046872	metal ion binding

Vocabulary: **biological process**
Term	Definition
GO:0006355	regulation of transcription, DNA-templated
GO:0006351	transcription, DNA-templated
GO:0006366	transcription from RNA polymerase II promoter
GO:0014807	regulation of somitogenesis
GO:0045944	positive regulation of transcription from RNA polymerase II promoter
GO:0048706	embryonic skeletal system development
GO:2000287	positive regulation of myotome development

Vocabulary: **cellular component**
Term	Definition
GO:0005634	nucleus

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001275	DM DNA-binding domain	PFAM	PF00751	DM	coord: 8..38 e-value: 6.9E-13 score: 48.3
IPR001275	DM DNA-binding domain	PROSITE	PS50809	DM_2	coord: 12..38 score: 10.396
IPR036407	DM DNA-binding domain superfamily	GENE3D	G3DSA:4.10.1040.10		coord: 4..38 e-value: 1.6E-13 score: 52.1
IPR036407	DM DNA-binding domain superfamily	SUPERFAMILY	SSF82927	Cysteine-rich DNA binding domain, (DM domain)	coord: 5..38
IPR026607	DMRT/protein doublesex/protein male abnormal 3	PANTHER	PTHR12322	DOUBLESEX AND MAB-3 RELATED TRANSCRIPTION FACTOR DMRT	coord: 5..38
None	No IPR available	PANTHER	PTHR12322:SF72	DOUBLESEX- AND MAB-3-RELATED TRANSCRIPTION FACTOR 2	coord: 5..38

Main menu

Dmd-3

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 6

Owlery

blazegraph-runner